LOCUS       DASVGB010000001         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_142047, whole genome shotgun sequence.
ACCESSION   DASVGB010000001 DASVGB010000000
VERSION     DASVGB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142047"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VF272_00005"
     CDS             complement(<1..290)
                     /locus_tag="VF272_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative folate metabolism gamma-glutamate
                     ligase"
                     /protein_id="HEX7259304.1"
                     /translation="MIVRPVATATVVLGGGTLPELLDTALSAMRERSILAVTSKVVSL
                     CEGRVAPMDQTDKASLVRQESQRYLPAEASRYEFELAIANNTLAPSAGIDAS"
     gene            complement(287..964)
                     /locus_tag="VF272_00010"
     CDS             complement(287..964)
                     /locus_tag="VF272_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX7259305.1"
                     /translation="MNIFGVKVRTFDMVMALYIFGVMTSEMLGAKTFPIATIGSFHLN
                     ATVAIFVLPLLFTLTDAVVEVYGRKRARSMIFAGLTTIVLLFLFLWLATSLPPSQRFA
                     EKEVAYDAIFGASMRIALASLAAFAIAELLDVAIFSKLRERMKQKALWFRNNASNFVS
                     QFVDSAVFLTLAFYAFDTSFGENFSFLLGLLIPYWLLRCLLSLLETPLVYAGVWWLRR
                     DKDADAT"
     gene            complement(1187..1336)
                     /locus_tag="VF272_00015"
     CDS             complement(1187..1336)
                     /locus_tag="VF272_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259306.1"
                     /translation="MRDVTLKGGRVQRIYFFAREVKDGAIDELPGGYIVVENPRTGLP
                     ILKKG"
     gene            1609..2109
                     /locus_tag="VF272_00020"
     CDS             1609..2109
                     /locus_tag="VF272_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259307.1"
                     /translation="MEPRQRSAQPPAHHPAPALSGGGSGGSSILNTVLVVLLIITSVL
                     VTAYLATKLIGSTAGNPAIKSKQTQALFLTNGQVYFGKLANINNSYVKLTDIYYLQVQ
                     QQVQPGQKTDSQTPQVSLAKLGGELHGPEDIMYVSREQVLFWENLKEDGKVAQAIKDY
                     KASGKK"
     gene            complement(2157..2687)
                     /locus_tag="VF272_00025"
     CDS             complement(2157..2687)
                     /locus_tag="VF272_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259308.1"
                     /translation="MKRAPIIFFIIAALAAWIGFIQLGSHQKQTAKLVQSIVAVDKAG
                     GNVSDKQRELIAYVRNHMRTSTSYMLEGSYGRAVSSAQAAAIPGGNGKVYADAQAACA
                     GRADSVAQARCVQNFLAAHVVPAANPQSVALPAKSEYSVKLSAPGWTADSTGLAFLLA
                     LASALLSVYLLIVRRI"
     gene            complement(2716..2970)
                     /locus_tag="VF272_00030"
     CDS             complement(2716..2970)
                     /locus_tag="VF272_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259309.1"
                     /translation="MDSREKLKIKVFSPYQIFFEGTGVSLSATNQTGPFDVLAGHSNF
                     FSVIKAGRVTVDTGFNPVIIEVDSGILRVTDNNVTLYANV"
     gene            complement(2973..>3349)
                     /locus_tag="VF272_00035"
     CDS             complement(2973..>3349)
                     /locus_tag="VF272_00035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7259310.1"
                     /translation="QEISAQLDSTIVLSRAVAESGVRPAVDLIHTTSSLLTPEIVGDR
                     HYLLATTVQAIMQKYESLKNIIAIIGESELSQVDRIDYQKAKKLIQFFTQDFFVSENL
                     TGRPGLYVSLPDTLQQLEEILI"
BASE COUNT          935 a          850 c          786 g          778 t
ORIGIN      
        1 ttcgaggcgt caatgccggc actgggagcc aaggtgttat tcgcaatcgc caactcaaac
       61 tcataacggc tagcctcggc cggcaggtag cgctgggact cctgccggac caagctagct
      121 ttatcggttt gatccatcgg tgcgactcgg ccttcgcaca gagaaacaac cttcgacgta
      181 accgccagga tgctgcgctc cctcatggcg ctcagggcgg tatctagcaa ttccggaagc
      241 gtcccgccac cgagcaccac cgttgccgtg gcaaccggtc ggacaatcat gtggcgtcag
      301 cgtccttgtc gcgcctgagc caccataccc cggcataaac caagggagtt tctagcaacg
      361 agagcaagca gcgcaacaac caatagggga ttagcagtcc gagcaagaag ctgaagttct
      421 cgccaaaaga ggtatcgaag gcgtagaaag ccaacgtcaa gaacaccgcc gaatcaacaa
      481 actgcgacac aaaattagag gcgttgttac ggaaccacaa cgctttctgc ttcatccttt
      541 ctcgtagttt gctgaaaatc gccacgtcca gtaattcagc aatcgcaaac gcagccaggc
      601 tagccaaggc gatccgcatt gaagcaccaa aaatggcatc atatgcgacc tccttttctg
      661 caaaccgctg cgaaggcgga agactggtgg cgagccacaa aaacaggaaa agcaggacaa
      721 ttgtggtcaa cccggcaaaa atcatacttc tcgccctttt tcttccgtac acctcaacaa
      781 cggcgtcggt gagcgtaaac agtaacggta aaacaaaaat ggcaaccgtt gcattcaaat
      841 gaaatgatcc gatggttgcg atgggaaagg ttttagcacc cagcatttcc gatgtcatga
      901 caccgaaaat atacagcgcc attaccatgt cgaaggttcg gaccttcact ccaaatatat
      961 tcatgatgtg ttatttccta accagttaag caataggatc tgcgcgccag atcttacctg
     1021 ctaaaaccgg accaggtagt aactcgcttg tttattaaca tgatcgtaat ccgatcttca
     1081 tagtaggctc aagtatacac tattctctat tcccaacgga aaattcggaa agccggccca
     1141 tcaaaaaaac cgcactgggc ggtttttttg ctcgaaagag gggaaattag cccttcttga
     1201 gaattggcag gccggtgcgt ggattttcta ccacaatgta gccgcctggc agctcatcaa
     1261 ttgcaccatc cttgacctcg cgggcaaaga agtaaatccg ctgtacgcgg ccacccttaa
     1321 gggtaacgtc gcgcatatgc aaataatatg tttgaccttt tgagttaacg tgcgaaaaag
     1381 ccatcggtac ctcacttata gttaatcttg agtttcagat taagctaacg gttatggcct
     1441 tgtcaacttt tatacctgaa aatttggttt ctacagatgg aaacccaaaa acttaaaaaa
     1501 tgcaaaaata atcgcttggc cgcagaatcg tgggttgtaa acataagctt gtttagctat
     1561 actggggtat aaagctagtg gaaataataa gttttaagtg gagtttttat ggaacctcgt
     1621 cagcgtagcg ctcaaccccc agcccatcat ccggcaccag cactcagtgg tggcggttca
     1681 ggcggtagta gtattcttaa taccgtactg gtagtcttac tgattatcac ctcggtgctg
     1741 gtaacggctt acctagcgac caagctaatc ggcagcaccg ccggcaatcc cgccattaag
     1801 tccaagcaaa ctcaagccct tttcttaacg aatggccagg tttacttcgg caagctagcc
     1861 aacattaaca atagttacgt taagttgacc gacatttact atctgcaagt tcagcagcaa
     1921 gttcagccag gtcagaagac cgacagccaa actcctcaag tttcgctggc taagctcggc
     1981 ggcgaattgc acggaccgga agacatcatg tatgtctcgc gcgagcaagt tctcttctgg
     2041 gaaaacctta aagaagatgg caaagtagcc caagctatca aggactacaa agccagcggt
     2101 aagaaataag tagcgaccgt ttaaatctaa aaaacctcct gtaaaaggga ggttttttag
     2161 attcgtcgaa cgatgagcaa ataaacactc agcagagctg atgccagcgc aagcaaaaaa
     2221 gcgagtccgg tcgaatcggc cgtccaacca ggcgccgaaa gtttaacgct atactcgctt
     2281 ttggcaggca gcgcaaccga ctggggattg gcggcgggga cgacatgggc ggctaaaaag
     2341 ttttgaacgc agcgagcttg agcaactgaa tcggcccggc cggcacaggc agcttgagca
     2401 tcggcgtaca ctttaccgtt gccgcccggg atagcggcag cttgggcgct cgaaaccgcc
     2461 cggccatagc tgccttccag catatagctg gttgaggttc tcatatgatt gcgcacatat
     2521 gcaataagtt cccgctgttt atcggatacg ttgccaccgg ctttatcgac tgcaacaatt
     2581 gattgcacca gcttcgcagt ctgtttttga tgcgatccaa gctgtataaa accgatccat
     2641 gcagccagag cagcaataat aaaaaagatg attggagcac gcttcatgcc tgctattcta
     2701 gcaccctacg cttcgttaga cgtttgcgta tagggttaca ttattatcgg tgactcgcag
     2761 aataccgcta tcaacctcaa taatgactgg attgaagccg gtgtcgaccg taacccgtcc
     2821 ggctttgata accgaaaaga agtttgagtg gccagccagt acatcgaatg gtccggtttg
     2881 attggtggcc gaaagcgaca cgccggtacc ttcaaaaaag atctgatacg gagagaaaac
     2941 tttaattttt aatttttcgc ggctatccat acttagatca aaatctcctc aagctgctgc
     3001 aatgtatcgg gcagcgacac atacaagcca ggcctgccgg tcaagttttc agatacgaag
     3061 aaatcttgcg tgaaaaactg aattaacttt ttcgcctttt ggtaatcaat cctgtcaacc
     3121 tggctaagtt cgctttcgcc aataatcgca ataatatttt tcaagctttc atatttctgc
     3181 atgatagctt gcaccgtggt cgccagcaaa taatgacggt ctccaacgat ttcgggagta
     3241 agcagcgaac ttgtggtgtg aataaggtca acggccggac gcaccccact ttcagccacg
     3301 gcacgcgaga gcacaatcgt tgaatcaagc tgagccgaaa tttcctgta
//
LOCUS       DASVGB010000002         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_994349, whole genome shotgun sequence.
ACCESSION   DASVGB010000002 DASVGB010000000
VERSION     DASVGB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994349"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VF272_00040"
     CDS             <1..262
                     /locus_tag="VF272_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7259311.1"
                     /translation="TIDIDGHNIQSIIDACAMAKTVDDKPTAIIAHTIPGKGVDYMEY
                     DYHWHGNPPGTLDVPGSPPKAEQVKAALDQLRALSERQGEPE"
     gene            259..1239
                     /locus_tag="VF272_00045"
     CDS             259..1239
                     /locus_tag="VF272_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX7259312.1"
                     /translation="MINPNMKLNDALLDPEVAQEPIRSGFGRGLLTAGERNDQIVALC
                     ADLTDSTKMTAFAKAFPERFVEVGVAEQNLVTVASGMAMMGKIPFTSSYAAFSPGRNW
                     EQIRTTICLNEANVKIVGSHAGVSVGPDGATHQMLEDIALMRVLPHMTVVVPCDAKEA
                     EKATLAIAELNGPAYLRLAREKSPVITSDETPFELGKVQVFREGADVTLVACGIMVHE
                     ALLAAQELANQGVEAEVINCPSIKPLDHEGLLASIKKTGKVVTCEEGQVAGGLGGVVA
                     ELVAENYPVPMRRVGMHDRFGESGKPHELLEHFGLTSPDIVKAVQELNSK"
     gene            complement(1242..1814)
                     /locus_tag="VF272_00050"
     CDS             complement(1242..1814)
                     /locus_tag="VF272_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7259313.1"
                     /translation="MPTFKPGVTAIVGPSGVGKNTVIAGLIQRYPQLHIGSSLTTRPA
                     RQNDLPGRYIHVSKDEFMSRDAAGELLEWAEYASHYYGSILPDKKDVNLILEIEYQGA
                     AQVRLKVPGARIVFMKPPGGTIEEQLAVLEERLAGRSTDTESARNERLVTARRELEIG
                     PTLADLVVVNEDVETTVDEIAAVMYTHLES"
BASE COUNT          529 a          482 c          543 g          524 t
ORIGIN      
        1 cacgattgat attgacggcc acaatatcca gagcatcatt gatgcttgcg ctatggctaa
       61 aacggtagat gataagccca cggcaattat tgcccatacg attccgggta aaggcgtaga
      121 ttatatggag tatgactatc attggcacgg caatccgccg ggaacgctcg atgtaccagg
      181 ctcgcctccc aaggcggagc aagtgaaagc agcgctagat cagcttcgtg ctttaagtga
      241 gcgtcagggg gaacccgaat gatcaaccca aacatgaagc ttaatgatgc tctgctggat
      301 ccggaagttg cccaagagcc aattcgcagc ggatttggac gcggtttact aacagccggt
      361 gaacgcaatg accaaatagt ggcgctgtgc gctgatctta ccgattcaac caaaatgacg
      421 gcatttgcca aagctttccc ggagcggttt gttgaagttg gagtggcgga acaaaatttg
      481 gttacggtgg cctctgggat ggctatgatg ggaaagattc cctttacttc cagctatgcc
      541 gcattttcgc cgggccgcaa ctgggagcaa atccgtacaa ctatatgcct gaatgaggca
      601 aatgtaaaaa tcgttggttc gcatgccggt gtgtcggtgg gtcccgacgg agcaacgcat
      661 caaatgttag aagatattgc actaatgcga gttttaccgc atatgacggt tgtggttcca
      721 tgtgacgcaa aagaggccga aaaggctacg cttgcgattg ctgagctcaa tggtccggct
      781 taccttcgcc tagccagaga aaaatcaccg gtgattacct cagatgaaac cccatttgag
      841 ctgggcaagg ttcaagtatt ccgagaagga gccgacgtaa ctcttgtggc atgcggcatt
      901 atggttcatg aggcgctttt ggcggcacag gagttggcca atcagggggt tgaagccgaa
      961 gttattaact gtccttcaat taaaccgctt gatcatgaag gacttttggc ttcaattaaa
     1021 aaaaccggta aggttgtaac ctgcgaagaa ggtcaagttg cgggaggcct gggcggggtt
     1081 gtggctgagc tggtggccga aaattatccg gttccgatgc ggcgcgtggg aatgcatgat
     1141 cggtttggtg aatcaggaaa gccgcatgaa ctattggagc atttcggttt aacatcgccc
     1201 gacattgtaa aggcagtgca agaacttaat tcaaagtagg cttatgattc gaggtgggta
     1261 tacattaccg ccgcaatttc atctacggtt gtctctacat cttcgttgac tactactaaa
     1321 tctgccaagg ttggaccaat ttcaagctct cgccgggcgg ttaccaaacg ttcgtttcgc
     1381 gctgattcgg tgtcggtact gcgtccggcc agccgttctt caagtacggc taactgttct
     1441 tcaatggtcc caccgggcgg cttcataaat acaatccgag ccccgggcac ctttaggcgg
     1501 acttgggccg cgccctggta ttcaatctcc aatataaggt ttacatcttt tttatctggt
     1561 aagattgagc cgtaataatg ggaggcatat tcggcccact ccagaagctc gcctgcggca
     1621 tcgcgactca taaactcatc tttactaaca tgaatatagc gtccaggcag atcattttga
     1681 cgggctgggc gagttgtcag ggatgaaccg atatgtagct gcgggtaacg ctgtatcaac
     1741 cctgcaataa ccgtattttt tccaacgccc gatggtccga caatggctgt tactccgggt
     1801 ttaaatgtag gcaacgaaga actccttcta gcatgcgtaa attttcttaa gtataccacc
     1861 cgccaagaca tgtgtctgaa ttggcacaaa accggcatac attgtacaca gctgatactc
     1921 aaaaatggac ctttttgggt gcgcttgtgc tgaacagttg atcaagttgc tgttttttat
     1981 aaccataacc gttataatga agcctatgga tccccaggat atccgcatta tttcaatcgg
     2041 agcggccacg caagacgttc aggttaccgg aaaggcac
//
LOCUS       DASVGB010000003         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1133637, whole genome shotgun sequence.
ACCESSION   DASVGB010000003 DASVGB010000000
VERSION     DASVGB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133637"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VF272_00055"
     CDS             complement(<1..675)
                     /locus_tag="VF272_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX7259314.1"
                     /translation="MPRKFIGIISYIFATVIVIGGTVLLVAYGNGYTYNFTSGKLVQR
                     GLLLLSSVPNGATITLGGKQLDQKSPYRNSFEQGWYDFTLGRDGYRTWHKSIQVKPSL
                     VSLHQYVILLRQRPLTDSIATHGSISQMVGSPDNRRVGFVVPHGDQAGLWSLDGENFE
                     QTRLLPLPVTADPVAPEVLSILSWSNDASHLLVRRVKGAENSLLVVSNNTSEAPLSIA
                     GIFNITP"
     gene            754..1761
                     /locus_tag="VF272_00060"
     CDS             754..1761
                     /locus_tag="VF272_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7259315.1"
                     /translation="MFGKKLAIDLGTTHVRVYEPKQGVIIDEPSVVAISTIDNSVMAI
                     GAEAKEMLGRTPDTIVASHPLKDGVIADYRITQAMIHHYIGKALGGFRLTRPDIMITV
                     PAGATSTERRAVIDATLAAGARAAYIIREPIAAALGANVPIASPAGNMVVNVGGGTTE
                     IAIMSLGGVVAQHSIRVGGNKLDAAIADGIRRSHSLAIGAQTAEEIKKEFASALIIKQ
                     DKKREIRGRDVVAGLPKTITINSHEITHAMADELEKIVLAIRAVLEKTPPELSSDIID
                     RGMLLTGGGALLGNLDKLLTNITGVPCVVAEEAQLCVVKGAGIALNNLDEYKRSLAGL
                     K"
     gene            1765..>2436
                     /locus_tag="VF272_00065"
     CDS             1765..>2436
                     /locus_tag="VF272_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259316.1"
                     /translation="MTAQAQSNDVTVWIQRGLLILIGLMPFHAFFSIWLGHLFGSQVL
                     WQSWKEILLLCLASAAALMLYRQPSRIKRLRTSFNYIFVAFIAVALAITVLHGPSPTV
                     ALFGIKTDIEFLLAFLLAQLVADTRFLGQAAKTLIISSGAVIGFGLLQIFLLPKDWLA
                     QFGYNAGTIQPYLPLDPAIDAVRIVATLGGPNQLGSFLILPHCLVVWRLLQNPRWWHA
                     LYIIVG"
BASE COUNT          582 a          622 c          640 g          592 t
ORIGIN      
        1 cggagtgata ttgaaaatac ccgcgatcga aagcggagct tcacttgtgt tgttggagac
       61 tactaacaag ctgttttcag cccctttcac gcggcgcacc agcagatgtg aggcatcatt
      121 ggaccagctc agaatactca agacttccgg agctacggga tcagcggtaa ccggcagagg
      181 caacagccgg gtttgttcaa aattttcgcc gtccagcgac caaagtccgg cctgatcacc
      241 atggggcacc acgaatccca ctcggcggtt atccgggctg ccaaccattt ggcttatgct
      301 gccgtgcgtc gcaatcgaat cggtaagtgg acgctggcgc aataaaatga catactgatg
      361 cagactcacc aaggaaggct tgacctgtat ggatttatgc caggtgcggt atccatcccg
      421 gcctaatgta aagtcatacc aaccctgttc gaaactgtta cgatacgggc ttttctgatc
      481 gagctgcttg ccgcccaagg taatggttgc accattaggg actgagctta ggagcaacag
      541 accgcgttga accagcttgc cagatgtaaa gttataggtg tacccattgc catatgccac
      601 caataaaaca gtgccgccga tgacaatgac ggttgcaaaa atatagctaa taataccaat
      661 aaattttcgc ggcataccga tcgtactata gtggatgcca agcgtttgcg ccagccatag
      721 ctggcaggcg ctaaaccaag tataatcaag gttatgttcg gaaaaaagct ggccatcgat
      781 ctaggcacaa ctcatgtacg ggtatatgag ccaaaacaag gcgtgattat tgatgagccg
      841 agtgtcgtgg caatcagtac gatcgataat tcggttatgg cgattggggc agaggccaag
      901 gagatgcttg gccggacacc tgatacgatt gtggcttccc atccgttaaa agatggggta
      961 attgccgatt accggattac ccaagccatg attcatcatt atatcgggaa agccttaggc
     1021 ggttttcggt taacccggcc cgacattatg attaccgtgc cggctggggc aacctctacc
     1081 gaacgccggg cggtgattga tgccacctta gctgccggtg cccgagccgc ctatattatc
     1141 cgcgaaccga ttgcagccgc gcttggtgcc aatgtaccga ttgcctcacc agccggcaat
     1201 atggtggtta atgtcggcgg cggtaccact gaaattgcca ttatgtcgct cggcggcgtg
     1261 gtcgcacagc attccatacg ggtgggcggc aataagttgg acgctgccat tgccgatggc
     1321 attcggcgtt cccatagctt ggcaattggc gctcaaactg ccgaggagat caaaaaggag
     1381 ttcgctagtg ccctaattat taagcaggac aaaaaacgcg aaatccgcgg acgcgacgtg
     1441 gtagccggct tacctaaaac cattaccatc aactctcatg agatcaccca cgccatggcc
     1501 gatgagttgg aaaaaattgt gctggcaatc cgggcagttc tggaaaaaac gccgccggaa
     1561 ctttcaagcg acattattga ccgcggcatg cttttaaccg gcgggggggc tttattggga
     1621 aatctggata agctcctcac caatattacc ggcgtgccgt gcgtggttgc cgaagaagcc
     1681 cagctgtgcg tagtgaaagg tgccggtatt gccttgaata atttggatga atataagcgc
     1741 agcttggcgg gacttaaata ggagatgacc gctcaagcgc agtcaaacga tgtgaccgtc
     1801 tggatccaac gaggtttact gatactgata ggcttaatgc catttcatgc ttttttcagt
     1861 atttggctgg gccacttgtt tggttcccaa gtcttgtggc agagctggaa agaaatccta
     1921 ttgctgtgcc tcgcgagcgc agcggctctt atgctgtacc gccaaccttc gcgcattaag
     1981 cgtttgcgga cctcctttaa ctacattttc gtggcattca ttgcggtagc ccttgcgatt
     2041 acagtgcttc atggcccatc tcccaccgtt gccctgttcg gcataaaaac cgatattgag
     2101 tttttgctag ctttcctgct tgcccagctc gttgctgaca ctaggttctt agggcaagcg
     2161 gccaaaaccc taattatcag ctccggtgcc gttatcggct tcggcctgtt gcagattttc
     2221 ctgcttccga aagattggct agcccagttt ggctacaatg caggcaccat tcagccttat
     2281 ttgccgcttg acccggcaat tgatgcggta cggatcgttg ccaccctggg agggcccaat
     2341 cagcttggat catttctgat actgccgcac tgcttagtcg tatggcgtct cctgcaaaat
     2401 ccgaggtggt ggcacgccct gtacataata gtcgga
//
LOCUS       DASVGB010000004         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1275193, whole genome shotgun sequence.
ACCESSION   DASVGB010000004 DASVGB010000000
VERSION     DASVGB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275193"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF272_00070"
     CDS             complement(<1..556)
                     /locus_tag="VF272_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7259317.1"
                     /translation="MEISYLGMNTVRLTGKDLAVLIDPYAKTSGLPDLKSHNNVILVS
                     SRLEDGTLPPGKSDMVIDRPGEYEIKGAMITGVPARLHIDEPTAPATATVYVLMVEGV
                     RIGYLGNIEPKLSNDQVEALSQVDVLILPVGGHGFTLDATAATQIISQLEPKYVIPTH
                     YDDEGVKYEVPQDKLEVFLKEIGAS"
     gene            complement(579..1247)
                     /locus_tag="VF272_00075"
     CDS             complement(579..1247)
                     /locus_tag="VF272_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7259318.1"
                     /translation="MFEQLFGSKTRVKLLSLFLNNPNRPYYVREITRKIDEQINSVRR
                     ELSNLLAIGIVRSDSNNNRLYYEVNQDYQFYAPLRAIFTNISLEEESDLKDTREDNEL
                     MKKLKATGNVQIAFETGTFVRDQNAGTDFFIMGDVNRARVGKFMNELEHDLGREINYT
                     VMTPEEFSYRQRLNDRFLASVMAAKKIILVDERSSKIKPAPASFADDIPEVAVPVEIR
                     ADNN"
     gene            complement(1333..>2235)
                     /locus_tag="VF272_00080"
     CDS             complement(1333..>2235)
                     /locus_tag="VF272_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259319.1"
                     /translation="QDIFISGAVTGGVRAAGQSVRITSPAIGGGVTAFSQNLTIDNGT
                     VISGGLTFASSNAIIRGSVAKSVSGAGSKIVIDGSLGKDLYAATGDLVLNKTALINGN
                     LHYYGSADLHKQQGARVVGATHHTRPSQDTQRDAVAATVFSAIWGLIAIYLVGAVLLW
                     LAPQFPVGVAAEISERPLASLGWGVVALLITPPLMILLLLTLIGIPMALILGAAYILG
                     LYLAHFWVGLALGEWGSAQAQWRPNPYANAFLGLLALTLIELLPFIGGTVAFLVVIIG
                     LGGLVLYAKRIAGKTARPAKTKAA"
BASE COUNT          555 a          588 c          540 g          552 t
ORIGIN      
        1 ggctagcgcc gatttctttc agaaaaacct caagcttgtc ctgcggtact tcgtacttca
       61 cgccttcatc gtcgtaatgc gttggaatca catattttgg ttcgagctgg ctaataattt
      121 gggttgcagc tgtcgcgtcc aaggtaaaac catggccgcc taccggaagg attaaaacat
      181 caacctgact taaggcttcc acttgatcgt tggaaagctt gggctcgata ttgccaagat
      241 agccaatgcg aaccccttct accattaaga catagactgt tgcggttgcc ggagcggttg
      301 gttcatcgat atgcaagcga gccggcacgc cggtaatcat ggcgccttta atttcatact
      361 cccccggccg gtcaatcacc atgtctgact tgcccggagg cagcgtcccg tcctcaaggc
      421 gtgaagagac taagattaca ttgttgtggc ttttaaggtc gggtaaaccg ctggtcttag
      481 cgtagggatc aatcagtacg gcaagatctt tcccggttag ccgcacagta ttcatcccta
      541 aatatgagat ttccatgatt acctcctgtt agttattttt agttattgtc agcccgaatt
      601 tctaccggta cagccacttc gggaatgtcg tcggcaaaag aagccggtgc gggcttaatc
      661 ttggacgaac gctcatcaac caaaataatc tttttagcag ccattacact ggctaaaaag
      721 cggtcgttga gacgctgacg ataggaaaac tcttctggcg tcataaccgt atagttaatc
      781 tcgcggccaa ggtcatgctc cagttcattc atgaactttc caacgcgtgc gcggttcacg
      841 tcacccataa taaagaagtc ggtgccggcg ttctgatcgc ggacaaatgt accggtttca
      901 aaggcgattt gaacattccc tgtagctttt agcttcttca tcagctcatt gtcttcccgg
      961 gtatctttca ggtcagactc ttcttccagg gaaatattgg tgaagatagc gcgcagcggc
     1021 gcataaaact ggtagtcttg gtttacttcg tagtacagcc ggttgttgtt gctgtccgaa
     1081 cggacaatgc cgatggccag caagttcgaa agctctcgcc ggaccgagtt aatttgttca
     1141 tcgattttgc gagtaatctc acggacgtaa tagggccggt tcgggttatt cagaaacagg
     1201 cttaataatt ttactcgggt cttcgagcca aacagttgtt cgaacaatac agctcctcgc
     1261 ttgcgttttt actatctgtt aaaagtatac caaacgatta taacccataa tagtcttggt
     1321 gttcagtgct acctaggccg ctttggtttt ggctggccgc gcggttttgc cagcaatgcg
     1381 tttggcatag agaaccagac cgccaagacc aatgattacg accaagaagg ctacggtgcc
     1441 gccgatgaat ggcagcagct caatcagcgt aagcgcaagc aaacccagaa aagcattggc
     1501 gtagggattg ggtcgccatt gagcctgcgc ggatccccac tccccaagcg ccagacccac
     1561 ccaaaaatga gccaagtaaa gcccaaggat ataggcagcg cctagaatca gcgccatcgg
     1621 aatcccgatc aaggtaagta agagcaggat cataagcggc ggggtaatca acagtgcaac
     1681 tactccccag cctaaactcg ccagtggccg ctcagagatt tcagctgcca ctccaaccgg
     1741 aaactgtggc gccaaccata gcagaaccgc acccaccaag taaatcgcaa taagccccca
     1801 gatggccgaa aatacggtag ccgctacggc atctcgctgg gtatcttggc tgggccgggt
     1861 atgatgagtt gcgcccacca ccctggctcc ctgctgctta tgtaggtcag cgctaccata
     1921 gtaatgaagg ttgccgttaa tcaaggctgt cttgttcagc accaaatctc cggttgcagc
     1981 atacaaatct ttacccagac tgccatcaat aacgatttta ctgccggcac cggagacgct
     2041 cttagccacg ctgccgcgga tgatggcatt ggatgaagca aaggtaagtc cgccgcttat
     2101 taccgttcca ttatctattg tgaggttctg cgaaaaagcc gttacgccgc cgcctattgc
     2161 cggactggta attcgtaccg actggccggc tgcacgcaca ccaccggtta ctgctccact
     2221 aataaatata tcctg
//
LOCUS       DASVGB010000005         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_560396, whole genome shotgun sequence.
ACCESSION   DASVGB010000005 DASVGB010000000
VERSION     DASVGB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560396"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VF272_00085"
     CDS             <1..1383
                     /locus_tag="VF272_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7259320.1"
                     /translation="FNRKDLKEIYEYCRKNTAVRYVIVQEVDRFMRSPDEQTYFYVKF
                     WYELKVRIWFADKPELNEDTHVASLLRYMEGWRAAGSNEERQSKSINGQTAALKEGRY
                     PFSPKPGYKRGYERGIQEPHEVRGPILKMFLGKIAAKQITPAQALSELNKTDFIKGRA
                     PFKMDKFRKIVADPFYAGIVEINKQVKVRNENGLHEALITKTQHLELVKIMADKKKVQ
                     SGPRKNGNPKYPLSNMVVCDLCLDRKNCRTVGYDHGNGKPNSKVYEKYRCRACMRRIT
                     RQELHTEVKYRFEANPISSGGRDDLVEALRVVWTQQEGQAKQDAVRIQHKINHLQEAI
                     SQQVEAATDPSNASIKEEILTSITKKKKEIKDLDDELDSLTNEAESDREEFLRFAFDF
                     VEDMGSNFLEITPENRARCKQIIFPAGFYWDSSNKVYTPEVSTLIRLAAKKKDAEASD
                     NSLLVRVQGL"
     gene            complement(1361..1436)
                     /locus_tag="VF272_00090"
     tRNA            complement(1361..1436)
                     /locus_tag="VF272_00090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1401..1403),aa:Gly,seq:gcc)
     gene            complement(1444..1519)
                     /locus_tag="VF272_00095"
     tRNA            complement(1444..1519)
                     /locus_tag="VF272_00095"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1484..1486),aa:Phe,seq:gaa)
     gene            complement(1651..2154)
                     /locus_tag="VF272_00100"
     CDS             complement(1651..2154)
                     /locus_tag="VF272_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259321.1"
                     /translation="MKTHQWWIWPLMLTGILVVVTAFVILFLPKTQFVHLEGPGTLKL
                     GSVVEVRVSFSQFLVDFRPSDSTVTLALFTEDMKEAGSFGVVRTDPGVDYRVINNVMW
                     WTAEGRSTGSEGIRLTIQLPDRLPDGAKELCFVATVKPQQRRNHVTSDEFCATIAPSK
                     IVQSERT"
     gene            2376..2819
                     /locus_tag="VF272_00105"
     CDS             2376..2819
                     /locus_tag="VF272_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259322.1"
                     /translation="MRSHAHSRITPTRVALRLLLALAIAVCIATVMASTGERKVGPAT
                     IGTATAQAADISCDDAWKDGNLVAIVFHDIQMFARKAGKCLKASNGSKVFVSYLYGEK
                     TLTKHLYPLCTHVRRKSPIFRATRAYVRQWPWNGFGQGCRRAGIL"
     gene            complement(2922..2998)
                     /locus_tag="VF272_00110"
     tRNA            complement(2922..2998)
                     /locus_tag="VF272_00110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2962..2964),aa:Val,seq:tac)
     gene            complement(3007..3093)
                     /locus_tag="VF272_00115"
     tRNA            complement(3007..3093)
                     /locus_tag="VF272_00115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3058..3060),aa:Leu,seq:gag)
     gene            complement(3135..>4505)
                     /locus_tag="VF272_00120"
     CDS             complement(3135..>4505)
                     /locus_tag="VF272_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7259323.1"
                     /translation="GAVVIFPAHMNRILRLSKDGVTVQPGVSIGTVQMALHTQGRVLA
                     PFSSNHYSSTLGGAVATNGGGGKSLKYGSVAAWVKRLKVVLDDGSIIETRRVSRREVD
                     RKKGIMTREGDLYRGIDGILQDHAGLIKESQLDVGRNTAGYRLSEVRGSGGSIDLSQL
                     FIGSEGTLGLITEITLVTERYEPRTTLVAGYFDDAEAAGEAILRLLKLHPSRIEFVGQ
                     ELLDVLYAHQPQLVAGVLPENSPHMVVMAEFDAVSHLRQNLLGRRAERLMGKLATGFH
                     ITTSRHEQSRLWEMRAAAMVILNSKQAPNRPVPIIDDVCVPVHKVSEFLVSTHKLLKK
                     HKRTAAVWGHGNGVMHIVPYMNLAKSKDRAEAFKLMDEFYGTVIKMGGTTAAERGDGV
                     VKGPYLERLYGREMADVLQQVKTLCDPNGIFNPEIKLGANKTTQENLLRQEFSTGHPY
                     DHLPHR"
BASE COUNT         1196 a         1183 c         1128 g          998 t
ORIGIN      
        1 tttaaccgca aagacctcaa ggaaatatat gagtactgta ggaagaatac ggcagtcagg
       61 tatgtgatag tacaagaagt tgataggttc atgagaagcc cagacgagca gacatatttt
      121 tatgtaaagt tttggtatga attgaaggtt cggatatggt ttgccgacaa acctgaactc
      181 aatgaggata ctcatgttgc aagcctattg aggtatatgg agggctggcg tgctgctgga
      241 agcaacgaag agcggcaaag caaatccatc aatggtcaaa ctgctgcctt aaaagaaggc
      301 aggtatccct tctctccaaa accaggctat aaacgtggtt acgagagagg tattcaagag
      361 cctcatgaag tccgcgggcc cattctaaag atgttccttg gaaagatagc ggcaaaacag
      421 ataacaccgg ctcaggcact ctcagaactt aataaaaccg acttcattaa aggtcgtgcg
      481 cccttcaaga tggataagtt caggaagatt gttgctgacc ccttttacgc tggaattgtt
      541 gagattaata agcaggtaaa ggtacgcaac gagaatggtc tacatgaggc cttaatcacg
      601 aagacacagc atcttgagct tgtaaagata atggcagata agaagaaagt tcagtctggc
      661 ccgcgcaaaa atggaaaccc caaataccct ctttcaaata tggtagtttg tgacctctgt
      721 cttgatagaa aaaattgcag aaccgtaggc tacgatcacg ggaacggcaa acccaactca
      781 aaagtgtatg aaaaatatcg ctgtcgcgcg tgcatgcggc gcataacccg ccaggaactt
      841 catactgagg ttaaatatcg ctttgaagct aacccaatca gctcaggcgg gcgagacgac
      901 ttggttgaag ccctgcgcgt tgtatggacg cagcaagaag gccaagccaa gcaagatgca
      961 gttcgtatcc aacacaaaat taatcattta caagaggcca ttagccagca agtagaggcg
     1021 gctactgacc ctagcaacgc atccatcaaa gaagagatcc tgacatcaat aactaagaag
     1081 aaaaaggaga ttaaggacct tgatgatgag ctggatagcc tcacgaacga ggcagaaagc
     1141 gatagagagg agttcctcag gtttgcattt gattttgttg aagatatggg cagtaatttc
     1201 ttggaaataa cacctgaaaa cagagcaagg tgtaaacaaa tcatttttcc ggctggtttt
     1261 tactgggact caagcaataa agtttacacg cccgaagtaa gcacccttat caggttagcg
     1321 gcaaagaaaa aggacgccga agcgtccgat aattcccttt tggtgcgggt acagggactc
     1381 taaccctgga cctcttcctt ggcaaggaag cgctctaaca actgagctac acccgcatgt
     1441 atttggtggc gagactcgga ctcgaaccga ggacacgagg ctcttcaagc cactgctcta
     1501 ccaactgagc tatctcgccg cgttagtggc gcgactaaag gataataccg aaaagccgat
     1561 tgtatttcaa cgcgtcagcc ttaaaaaccg atgcggcgag cggtcatact tgacgaccgc
     1621 tcgctcgctt gccgcatcgc tacggcgttc ttatgtcctt tctgactgga caatcttgga
     1681 tggtgcgatc gtcgcgcaga actcgtcaga ggtcacatga ttcctccgct gctgaggctt
     1741 caccgtcgcc acgaagcaga gctcctttgc gccgtctgga agccggtcgg gcaactgaat
     1801 ggtgagacgg atgccctcag agcccgtgct cctgccctcc gccgtccacc acatcacgtt
     1861 gttgatgacg cgatagtcga cgccggggtc ggtgcgaact actccaaatg acccggcttc
     1921 cttcatgtcc tcggtgaaga gagcaagggt cacggtggaa tcgctcggac gaaaatcaac
     1981 caggaactgg ctgaacgaga cgcgcacctc caccacgctg cccagcttca acgtgccggg
     2041 accttcgagg tgaacaaact gcgtcttggg gaggaacagg atgacgaaag ccgtcaccac
     2101 gaccagaatg cccgtaagca tcaatggcca aatccaccat tggtgtgttt tcatgacgaa
     2161 ccctttcgaa gaagaacaag acagaaagga gataaaaaag aacaccctgg aagagcgaat
     2221 ttttcactcc ccagagttag ttatattgta taataaattt cagaaatatc aacgtgtcaa
     2281 ccgacattga cataattata agaagtggta agatactata ctgacgtgta aatcaagcgt
     2341 cagcacatcc gtattgtcta gtaggaggtt agggtatgcg ctcccacgcg cacagccgca
     2401 tcacccccac tcgtgtggcc ctcaggctgc ttctggcgct ggccatcgct gtctgcatcg
     2461 ccaccgttat ggcaagcacc ggcgaacgca aggtcgggcc ggccacgatc gggaccgcta
     2521 cggctcaggc tgctgacatc agctgcgacg atgcttggaa agacggcaac ctggttgcaa
     2581 tcgtgttcca cgacatccag atgttcgctc gtaaggcggg caagtgcttg aaggccagca
     2641 atggcagcaa ggtcttcgtc tcctacctct acggcgagaa gacgctgacc aagcacctct
     2701 accctctgtg cacccatgtg cgaaggaaga gtccgatctt cagagcaacc cgcgcctacg
     2761 tccgacagtg gccatggaac ggcttcgggc agggctgtcg ccgtgccggc attctgtaac
     2821 acgccgtcaa ctggcccggg ggaacggatt cccccggccg gtcggaaggc tctatacgag
     2881 tcttccgacc tacccagcaa ttattctgct tttataatgc ctggtgggcg atgagggatt
     2941 cgaacccccg accccctcgg tgtaaacgag gtgctctaac cagctgagct aatcgcccaa
     3001 atgttttggt gcggacgaga ggacttgaac ctccacgacc taagcggcca ccagcccctc
     3061 aagctggcgt gtctaccatt ccaccacgtc cgcgcatccg gtatttgttg accctaggaa
     3121 tagtagcatg gattttagcg atgcgggaga tgatcgtagg ggtggccggt actaaattcc
     3181 tggcgaagta ggttttcctg ggtggtttta ttcgcaccca attttatttc agggttaaaa
     3241 atgccgttgg gatcacacaa ggttttaact tgctgcaata catccgccat ctcgcggcca
     3301 tacaaccgct ctaaataagg cccctttact acgccatcac ctcgctccgc agccgttgta
     3361 ccacccattt taataaccgt accgtaaaac tcatccatca acttaaaagc ctccgcgcga
     3421 tccttggact tggctagatt catataagga acaatgtgca ttacaccatt gccgtggccc
     3481 caaacggccg ccgtacgttt atgctttttt agcaatttat gggtcgacac taaaaattcg
     3541 gacactttat gaaccggtac gcagacatca tcaattattg gaacagggcg attaggtgct
     3601 tgcttggaat taagaataac catagcagcg gcgcgcattt cccacaaacg agactgctca
     3661 tgacgactgg ttgtgatgtg aaagccggtc gccagcttac ccatcaaccg ctcagcccgg
     3721 cggccaagaa ggttctgccg caaatgtgaa accgcatcaa attcggccat aaccaccatg
     3781 tgcggggaat tttctggcaa aacgccggct accagctgcg gttgatgggc gtaaagcaca
     3841 tccaacagtt cttggccgac aaactcaatg cggctagggt gaagtttgag taatcgcaga
     3901 atcgcctcac cggccgcttc agcatcgtca aaataaccgg ctacaagggt tgttcgaggt
     3961 tcgtagcgct ccgttacgag ggtgatctcg gtaataagac caagcgtgcc ttcagaccct
     4021 ataaacaact ggctaagatc aatcgagccg ccggagccgc gcacctcgct cagccggtat
     4081 cctgccgtat ttcttccaac atccagctgg ctttctttaa ttaaccccgc gtggtcttgg
     4141 agaataccat cgatgccgcg gtacagatcg ccctcgcgtg tcattatccc ctttttccgg
     4201 tcaacctcgc ggcggctaac tcggcgggtt tcaattattg aaccatcgtc cagtacgacc
     4261 ttgagccgct ttacccaagc cgcaaccgag ccgtacttca ggctcttacc gcccccgcca
     4321 ttggttgcta ccgctccgcc aagtgtcgag ctatagtgat tagaggaaaa cggagctaaa
     4381 actcggcctt gggtatgcag cgccatttga accgttccta tcgagactcc cggttgaacc
     4441 gtcacgccgt ccttagataa gcgcaggatt cggttcatat gggctggaaa gatgacaacg
     4501 gcacc
//
LOCUS       DASVGB010000006         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_558861, whole genome shotgun sequence.
ACCESSION   DASVGB010000006 DASVGB010000000
VERSION     DASVGB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558861"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF272_00125"
     CDS             <1..267
                     /locus_tag="VF272_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259324.1"
                     /translation="FKNWRLLKVVQPHSKTDHLAYLFLATDFESQTDPKPDPGEKIIV
                     HLKTLDELKQMLNLANTRYLPQDILARVATIEQLEALPAYDPSA"
     gene            complement(264..1040)
                     /locus_tag="VF272_00130"
     CDS             complement(264..1040)
                     /locus_tag="VF272_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7259325.1"
                     /translation="MTLWYRRYLPFGTVLRRECQRFWSIRRQTILGPLLETYLYISVF
                     GAALGSRIKDLEGHSYIEFIIPGLLLMAVAINAFSNNSSSLLQQKLQRAIDDQLASPV
                     SNTALLAAFTLGGFIRGLLIAAITFITASFLIDLPVAHPLLLLGSLVICGLFFASLGV
                     LVGLRGETFDNISFYQTFILQPLIFLGGIFYSVKLLPEPFKTITLFDPMLYMINTVRY
                     GMLGSADVNPYLSLIIIFGATVGLIGLNMYLFKKGYRLRS"
     gene            complement(1037..1807)
                     /locus_tag="VF272_00135"
     CDS             complement(1037..1807)
                     /locus_tag="VF272_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7259326.1"
                     /translation="MAIKSSKQKALKLHQLRKRYKSGVEALKGVDLEVEQGGFLGLLG
                     PNGAGKSTLIHCIMGLAIPTSGSGSVFGHDIVDDYQEARRLCGLAPQEINMDWFLTVE
                     DTLDFHGGYYGMTKAERQPRIDELLETFSLTDKRKAKMLALSGGMKRRVMLARAVMHN
                     PKVLILDEPTAGVDVELRLELWQYMRKINKAGTTIILTTHYIEEAEGLCKQIALINKG
                     EIVEEGTSAQLKKQFGKKTLEDVYLAVTGREELARVAK"
     gene            complement(1924..2412)
                     /locus_tag="VF272_00140"
     CDS             complement(1924..2412)
                     /locus_tag="VF272_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259327.1"
                     /translation="MVLKLYTVFVALLLSLLVGVGVSAFYPAPKAPDYPIMLDGQTKP
                     AVDSAEHETTRLAYEKQQKEFQKNNSEYNRNVSLITLTAAVLLLAIGLTLFRATVLMA
                     DGLTLGAVFTLIYSIIRGFGTDDTQFRFLMVLCGLAVALLLGYIKFVKPATAGKPASP
                     KK"
     gene            2539..2961
                     /locus_tag="VF272_00145"
     CDS             2539..2961
                     /locus_tag="VF272_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX7259328.1"
                     /translation="MNVEVNVWAVVLAMLSSLVVGSVWYARSVFGNTWIKLAKIDMGK
                     DGGSVFKPIAITMVVSLITAYVLAHVSFLSHEYFNNSFLQDSLTTAFWMWLGFTAARF
                     ITHDAFEGRPAKLTLLNISNEFVTFMVMGLVIGLMGIG"
     gene            complement(2944..3951)
                     /locus_tag="VF272_00150"
     CDS             complement(2944..3951)
                     /locus_tag="VF272_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7259329.1"
                     /translation="MKTAAPTNSKAGAKLGLVVGKFYPPHKGHHFLINTAKQHCDRLI
                     VMVVEGKNQHPDGSTRMQWLKTAHPDVEVRLIEDIYDDDNSQAWAAYTMKLLGRSPDI
                     VFTSETYGDPWARAMGSEHMLVDLERKVVPISASMVRSDPLKKWQFLDPPVRAHYGKR
                     VVIVGAESTGKTTLATALADHYKTSWVPEYGRMYTGGKITSEAGAEWESEEFRFIAEE
                     QNRLEDMLAGYSNKLLICDTDAVATAVWHEHYMKSWSKEVEALYENRDNAFYLVTDYH
                     IPYKQDSIRVGEDSRSLMHERFVELLDKLGRPYVVISGTPEERLKQAVTQCDAVLADP
                     H"
BASE COUNT         1067 a         1034 c          921 g         1035 t
ORIGIN      
        1 tttaagaact ggcgcttgtt aaaggtagtc cagccgcata gcaaaaccga tcatctagct
       61 tacctgtttc ttgccaccga tttcgaatcc caaaccgatc ctaagcccga tcccggagaa
      121 aaaattatcg ttcatttaaa gaccttagac gaattaaagc aaatgctgaa ccttgccaac
      181 acccgctacc ttcctcaaga catcttggcg cgcgttgcta ccattgagca actggaagcc
      241 ctccccgcgt acgacccatc cgcttagctg cgaagacgat atcccttttt aaacaagtac
      301 atattcagcc cgattaaacc gacggttgcg ccaaagatga taattaatga aaggtatgga
      361 ttcacgtcgg cgcttcccag cattccgtaa cgaacggtgt taatcatata cagcatcgga
      421 tcgaaaagcg taatcgtttt aaaaggttca ggcagtagtt ttaccgaata aaaaatgcct
      481 ccaagaaaaa ttaacggttg caaaataaaa gtttgataaa aactgatatt atcaaaggtt
      541 tcgccgcgca aacccactag cacaccgagt gaagcgaaga aaagtccgca aattaccaga
      601 ctgcctagca gtagcaatgg atgcgctacc ggcagatcaa tcaggaatga ggctgttata
      661 aaggtgatcg cagcaattaa tagtccccgg ataaacccgc ccagggtgaa ggcggctaaa
      721 agggccgtgt tggaaaccgg cgaggccagc tgatcatcaa ttgctcgctg aagtttttgt
      781 tgcaagagcg atgatgagtt attggaaaaa gcattaatgg cgacggccat caatagcaat
      841 cctggaataa tgaattcaat gtacgaatgg ccttccaagt ctttaatccg gctacccaag
      901 gcagcaccaa acacgctgat gtataagtaa gtttcaagca gcggccccag aatggtttgg
      961 cggcggattg accaaaaccg ctgacattcg cggcgcaaaa ccgtcccaaa aggtaaatat
     1021 cggcgatacc acaacgtcat tttgctaccc gtgcgagctc ttcgcgtccg gtaactgcca
     1081 ggtacacatc ctccagagtc ttcttgccga actgcttctt caattgggca gatgtaccct
     1141 cctcaacaat ctcgccttta tttataagag cgatctgctt gcaaagtccc tccgcctctt
     1201 caatataatg agttgtaagg atgatggttg tcccggcctt attaattttc ctcatgtact
     1261 gccatagctc caagcgcagc tcaacatcaa cccccgccgt cggttcgtcc agaatcagca
     1321 ccttggggtt gtgcattacc gcccgggcta gcattacccg gcgcttcatg ccgccggaaa
     1381 gcgctaacat tttcgcctta cgcttatcgg ttaatgaaaa cgtttcgagc aattcatcaa
     1441 tccgcggctg gcgttcggcc ttggtcatac cataataacc accatgaaaa tccagcgtat
     1501 cttcaaccgt taagaaccag tccatattaa tttcttgcgg cgccaacccg cacaaacggc
     1561 gggcttcttg ataatcatca acaatatcgt ggccaaacac gctgccactg ccgctggtgg
     1621 gaatcgcgag ccccataatg cagtggataa gtgtcgattt gccagccccg ttaggaccga
     1681 gcaagccaag gaagccgcct tgctccactt caagatcaac tccctttaaa gcttcaaccc
     1741 cgcttttgta gcgcttgcgc agctgatgca gtttcagcgc tttttgtttt gatgatttga
     1801 tcgccatata ggttatcagt gtagcaaaat actagtccag acgcactgag cgccctaaca
     1861 aaacaggagg ctcaatcgga ggacgctcga agcgcttcgt caagttaaaa gcaagcgcga
     1921 tgattatttt ttgggtgaag ctggtttgcc ggcggtagcg ggcttcacaa attttatgta
     1981 gcccaaaagc aaggccaccg ctagcccgca aagcaccatt aagaagcgaa actgggtatc
     2041 atcggtacca aaaccgcgga taatactata aatgagggta aatacagcgc ccaaggttaa
     2101 tccatcggcc attaacactg ttgcccggaa gagggtgagc ccaatggcga gcagtagcac
     2161 cgcagccgtc agcgtaatta aggaaacgtt ccggttgtac tcagaattgt tcttttggaa
     2221 ctctttttgc tgtttttcat aggcaagacg cgtcgtctcg tgttcagcag aatccaccgc
     2281 gggcttggtc tgtccatcta acataatcgg ataatcgggt gcttttggtg cgggataaaa
     2341 cgccgaaacg ccaaccccga ctaagagtga aagtaatagt gccacgaaga cggtatatag
     2401 ttttagtacc acgatagcta cccttccaat tattacttat agaatactac ttatggctca
     2461 ttcaagctaa taaagactta aaatatacta tactttcaag cttttgctac aatcaaatta
     2521 ctaacaagga gagatcctat gaatgtggaa gtcaatgtat gggccgtagt gctagcaatg
     2581 ctcagttcgt tagtggttgg tagtgtgtgg tacgcccgat cggtattcgg caatacctgg
     2641 ataaaactgg ctaaaatcga tatggggaaa gatggtggca gcgtgttcaa gccgattgcc
     2701 attacgatgg ttgtcagcct gattacggct tacgtgcttg cccatgtatc atttttgtcg
     2761 catgagtatt tcaataacag ctttcttcaa gactcgctaa ccaccgcatt ttggatgtgg
     2821 cttgggttta ccgctgctcg gtttattacg catgacgcgt ttgagggacg cccggcgaaa
     2881 ttaacgcttc tcaacatatc caatgagttc gtgaccttta tggttatggg tctggttatc
     2941 ggcttaatgg ggatcggcta acaccgcatc gcattgagta acggcttgct taaggcgttc
     3001 ttcgggagtc ccgctgatta ccacataggg ccttccaagc ttatccaata attccacaaa
     3061 ccgttcatgc attaaggagc ggctgtcttc acccactcga atactgtctt gcttataggg
     3121 aatatgataa tccgttacta gatagaaggc gttgtcgcga ttctcataca gggcttcaac
     3181 ttccttggac caacttttca tgtaatgctc gtgccagacc gcggttgcca cggcatcggt
     3241 atcacaaatc agcagcttgt tactataccc cgccaacatg tcttccaggc gattttgctc
     3301 ctcggcgata aagcgaaatt cttcgctttc ccactcagcc ccggcctctg atgtgatctt
     3361 gccaccggta tacatgcgac catactcagg cacccagctg gttttataat ggtcagccag
     3421 agcggttgcc agggtggttt tgccggtcga ttcggcaccg actatcacca cccgcttacc
     3481 ataatgtgct cgcaccggcg gatctaaaaa ctgccatttc tttaatgggt ccgatcgcac
     3541 catcgaagcc gaaatcggaa caaccttgcg ttctaaatca acgagcatat gctccgaacc
     3601 cattgcccgt gcccatggat cgccataggt ctccgaagta aagacaatgt ccggcgagcg
     3661 gccaagcagt ttcatggtat aggccgccca tgcttgagaa ttatcatcat cataaatatc
     3721 ttcgatcagc ctgacctcaa catccggatg tgctgttttc agccattgca tgcgcgtcga
     3781 tccgtcagga tgctgatttt taccttctac taccattaca attaacctgt cgcagtgctg
     3841 tttggcagta ttaattaaaa aatgatgccc tttatggggc gggtaaaatt tgccgacaac
     3901 caagccaagc tttgcaccgg ccttgctatt agtaggggcg gcggtcttca aattcttgcg
     3961 tccactcgcc ttttgcggct aggctattgg cttcgacacg gtgcaggtaa gcagcgcgag
     4021 ctttatcgat gtctccttgt gcccataacg tcagtac
//
LOCUS       DASVGB010000007         7458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_982890, whole genome shotgun sequence.
ACCESSION   DASVGB010000007 DASVGB010000000
VERSION     DASVGB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982890"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VF272_00155"
     CDS             <1..933
                     /locus_tag="VF272_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259330.1"
                     /translation="NGIAGEVSGQQAASTSFRLNSGNNQTQQAHVPLAPTFDNPANYY
                     SKLHFVINPSNNPSDTKFAIAISSDSFATTRYIQNDGTVGSTLGIEDYQIYGSTGVGW
                     GGASGSLVIGLESSMTYQIKAKAISGKFTETGFGPLASATTSPPSLFFDIDVSASDTE
                     TGSPYQMSFGSLLSGTVIDGPQKIWFDLDTNAEYGARIYVSGNTAGLTSQSASNTIIS
                     ATADLSAAPEGYGAQSSSAAQTSGGPLTAASPYDGAGSNIGIMNTTLRQFYSSPGPIS
                     SGRSSLLLKAKAGVQTPSADDYQSIMTAIAAASF"
     gene            1064..2095
                     /locus_tag="VF272_00160"
     CDS             1064..2095
                     /locus_tag="VF272_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259331.1"
                     /translation="MKKHFRCRFSVGVVVVIVLGILAPIPWHPAYAVSLTDTYLRMNR
                     MKASQATDFRIVFKPATAVAANGKVKVTFPAGFTVNATQTASSASCAAESGATAMPGV
                     SAPSGSGQTISVTTTAGLSGGTLYCVDLTSTTAVTNHATPGGYVPTVFTTTSGDVTID
                     SKTVGLRVIADDQIVISAVVPPTFDFALSGNTDAFTALLDPTAVRTTTGRTITISTNA
                     AKGWIAWVKDSNQGLSSASAAYTIPTTGTVNGTPDTLSAGTEGYVLDVNLTTDSGNGT
                     PSVSAEYNGVAANSGGTLSGTFQPMATSTGTALGDVITLIEYAAINGETPAGDDYTDT
                     LTVVGAGYF"
     gene            2358..3500
                     /locus_tag="VF272_00165"
     CDS             2358..3500
                     /locus_tag="VF272_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259332.1"
                     /translation="MNRTSLIVLSVVALLVQIVPVHLIPNAYALTTTDTYLRLNRMKS
                     STGTAQPTTFRLVFKTPATDTGSDAKLQVQFYHPNNADPSQADNNFTINATQAVATAN
                     CATEASNELSASVLALPGTLSASGDNSGSGATRKTITITGVTNLTTSQFYCVDFTTSN
                     AVTNPVNPNLYMVQIRTLTSADAFLDTKLVGVRIVDPIDANYKYDQITVTGVVPPSFS
                     FNLSGNTDAFTALLDPAAVTYTTGRTVTISTNAAKGWIVWVKDSDQGLKSVSTPSSVI
                     ASTGTLSDDLIDTAAPGTENFLLDVDLTTDGAGGGTVTVDPDYDGTASGEGGALSNTI
                     FQQVAHASGTASGDVITLKELATITGSTPAGDDYTDILTVIGAGNF"
     gene            3565..4638
                     /locus_tag="VF272_00170"
     CDS             3565..4638
                     /locus_tag="VF272_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259333.1"
                     /translation="MQHKILVERIICIMLVATISVASLCVPFGAQAGTESTQPGDLHL
                     ITSPLPILLTVKPGTTTTTDLRIKNAGSTTERLRVDMLKFGASGEEGQPALKDPEPGD
                     EQFKWASFSQTIFNAEPNEWKTVKMTIKVPTTAAYGYYYAITFSRANKEAKTTDQRAV
                     LRGATAVLVLLEATSPNTKRKVQMVDFVSNKGLYEYLPASFKVRLHNIGNIHTAPVGS
                     VFITKGSKPIGSMSINKEHGSILPNSYRIYQTEWKDGFPHYAEAVENNQTAINKKGQQ
                     EKRLVWNLGDIGKIRFGKYTASVVMVYDDGKRDIPVEAAVSFWVIPWRFLLIVGLVVT
                     LMLAGLWAMVRVVRPSRRAKRRR"
     gene            4641..5624
                     /locus_tag="VF272_00175"
     CDS             4641..5624
                     /locus_tag="VF272_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259334.1"
                     /translation="MNRSIARSLFFGFLIILGCYWIWSHPVQAESSGGVVISPPFQQV
                     VVGDGKGTAFDVTVTNRTTSDQSITATTVDFKSLDESGGVAFLGSEISELERKYGLAN
                     WLKLDAAPTIIPAGASKKIVVNLENREDLTPGGHYGAVIFKIEGDNASENSNSVDVKQ
                     VLASLIFAKKLGGERYDLKLSSLDPPPKLASGDQKVKLRFYNPGNVHVVPRGILRLKD
                     SKGKVITTSLINQESGILLPESYRVYPVILKNIPVLPAGKYVLEAEYRYDGIEQTAKL
                     TKEITIFDWRYAAFLGAVGSVSLVPLLLIRKFIAHRRRKKHKGRRKHYAAF"
     gene            5728..>7458
                     /locus_tag="VF272_00180"
     CDS             5728..>7458
                     /locus_tag="VF272_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259335.1"
                     /translation="MRATVILGKFKRTNSMMLVGLFFAVVSLNAFLQPRTAEAATGIN
                     QQINFQGKVVNPDGTNIPNGTYNIEFKIYQDGNGVLGGGDETLKWTESRLRNNSQGVT
                     ITDGIFQVNLGSVNTALGTAVDFNQDTLWLSINLGNTNATCTPFSGCSPDGEMNPFIR
                     FTSAPYALNSGKLSGLTSSQFVQLAQGVQVDASSASSIFINKTGASGNLLELQRAGTD
                     VLAVDNNGTATLQAAAGNNATLTVKQASTATSGDIFSVTGSNGTSKFIQVTSTAANQG
                     SVIVQSLGSNTLTVDSGSGTLVVGTTTTTIQKSGAALTIDLATAAASTLTITNSNASN
                     TASLSVEGGGSFGGNVLVKTDSASAFQVQTSGSTNLFNIDSTTGDITSNHGTLTVNGA
                     NNPTNVVISGSPSGGTLAASTTFAYRVAATDISGSITQAVAGTPTSYTSGAGPNNNQV
                     AVSWSAGTGAQSYRIYRTTNGGTNWFVNTVSSATTSINDNGTNFTWSTAGTPPTTNAT
                     GGVTLAGGTPLIFNGNGGTVTLQPSISVTSSYGLSLPTGQGGVNTVLLNDGSGNLTWV
                     GTAGCSTCVAT"
BASE COUNT         1943 a         1829 c         1838 g         1848 t
ORIGIN      
        1 aatggtatag ccggagaggt aagcggtcag caggctgcaa gtaccagttt tcggcttaat
       61 tccggtaata atcaaaccca acaagcccac gtcccattag cgccaacatt tgataatccg
      121 gcaaactatt acagcaagct gcattttgtt attaacccct caaacaatcc atcggataca
      181 aaattcgcga tcgcaatcag ttccgacagc tttgcgacga ctcggtatat tcaaaatgac
      241 gggacggttg gctcaaccct cggcattgaa gattatcaga tatatggctc gacgggagtc
      301 ggttggggag gggcttccgg atcattggtg atcggacttg aatccagtat gacctatcaa
      361 attaaggcca aggcaataag cggaaaattt accgaaaccg gctttgggcc tctcgcaagc
      421 gctacaacct cgccgccgag cttatttttc gatattgatg tttctgcttc cgacaccgaa
      481 accggttctc catatcagat gagtttcggt agtttgcttt cgggaacagt gattgatggt
      541 ccgcagaaaa tctggttcga ccttgatacg aacgccgaat acggtgcgcg catatatgtt
      601 tccggcaata cagcaggatt aaccagccag tctgctagta acactattat ttccgcaact
      661 gccgacttaa gtgccgctcc cgaaggctat ggagctcaga gctcatcggc agcacaaacc
      721 tcaggggggc cgcttacggc tgccagtcct tatgacggcg ccggtagtaa catcggcatt
      781 atgaatacca cgttgcgcca attctactcg tcgccgggtc ccatttcatc tggccgttca
      841 tcgctgctgc ttaaggccaa ggctggcgtt cagacaccaa gcgctgatga ttatcagtca
      901 attatgaccg ctattgccgc cgccagcttc taatatttca gttttttaga cattgttgat
      961 ccgttataac cataactgga ttgacagcat aagcgttatt gtagacaata taagataagt
     1021 gccgtgtgtg gcaccaaggt tggatgaata aggtgggttt attttgaaga agcactttag
     1081 gtgcagattt tcggtcggag tagttgtcgt tattgtactc ggtatactag cgcctattcc
     1141 atggcaccca gcgtatgctg tttctttaac cgacacctat ttgcgaatga atcgcatgaa
     1201 ggccagtcag gcaaccgatt ttcgcatcgt atttaagccg gccaccgcgg ttgccgccaa
     1261 cggcaaagtt aaagtaacct tccctgcagg atttacggtt aatgctacgc agacggctag
     1321 ttcggcctcc tgcgctgctg aatccggcgc aactgccatg cccggtgttt ctgcaccgag
     1381 cggtagcggc caaaccatat ctgttacgac aacagccggt ttgagcggcg gcactcttta
     1441 ctgcgttgac ctaacaagca ctaccgctgt taccaatcat gcgacgccag gcgggtacgt
     1501 tccaacggtt ttcaccacta cctcaggtga tgtcactatt gatagtaaaa cggttggatt
     1561 gcgggtgatt gccgatgacc aaattgttat ttcagcggtt gtcccgccaa catttgattt
     1621 tgccttgagc ggcaataccg atgcctttac tgccctactt gatcccactg cggtgaggac
     1681 aactaccggg cgaacaatca ctatctctac taatgctgcc aagggatgga ttgcttgggt
     1741 taaagacagt aatcagggac tttcctcggc aagtgccgcc tataccattc ctactaccgg
     1801 tactgtgaac ggtacgcccg acaccttaag cgccggtact gaaggatatg tgcttgacgt
     1861 aaacctaaca accgatagcg gcaacggaac tccttcggtt tcagctgaat ataacggggt
     1921 agctgctaac tcaggcggta cattgagcgg gacgttccag ccgatggcaa cttctaccgg
     1981 cactgctttg ggtgacgtca taacccttat tgagtatgcg gcaattaatg gtgagacccc
     2041 tgctggggac gattatactg atacgctgac cgttgttggt gccggctact tctagcaatt
     2101 atcaaatttc tatataccta ggtttctatt tataagcatt atgcaaattg cgtaatgctt
     2161 atgctataat aagagtacat ccagaggtgg atgttacaga ctgatagccc gagacaggga
     2221 aattggtaga ttttgtgttg gaaaagtatt gacatgatgc ttatgcttcg tttatagtga
     2281 agcgtaacag ctttaaaaca aaaaccgaag ctgaaacata aacaaaaaga aaacacagaa
     2341 tgcgaattaa atacacaatg aatcgaacat ccctgatcgt cctgagcgtc gttgcgctgc
     2401 tagtccaaat tgtaccggtt cacttaatcc cgaatgcata cgctttgacg acgaccgata
     2461 cctacttgcg actgaatcgt atgaaatcaa gtacgggaac cgctcagccg acaaccttcc
     2521 gtctagtctt taaaacacca gctaccgata ccggatctga tgcaaagttg caagtacagt
     2581 tctatcaccc caataacgcc gatccgagtc aagcagataa caacttcact attaatgcta
     2641 cgcaggccgt tgcgacagcc aactgcgcaa ctgaggccag taatgagtta tcggccagcg
     2701 tcttggccct tccgggaacg ctcagcgcca gcggtgacaa ctcaggctca ggagcaacac
     2761 gtaaaaccat tacgattacc ggggttacga acctcactac ctcccagttc tactgcgttg
     2821 actttacaac ctccaatgcc gtgaccaacc cggtgaaccc caacctctac atggtgcaga
     2881 ttcgaactct cacgtcggcc gatgctttcc ttgacaccaa attagtcggt gtaagaattg
     2941 ttgaccctat cgacgccaac tacaagtatg accagattac cgttaccggt gtagttcctc
     3001 cgtccttcag ctttaacctg agcggtaaca ccgatgcctt cacagccctg ctcgacccgg
     3061 ctgcggttac ctacacgacc ggccgaacgg taaccatatc aaccaatgct gctaaaggat
     3121 ggattgtgtg ggtcaaagat tctgatcagg gcttaaagtc ggtatcgacg ccaagctcag
     3181 tgattgcttc aaccggtact ctcagcgacg atctgataga cacggccgcc ccaggaaccg
     3241 agaatttcct cctagatgtt gatttaacca ctgatggtgc tggcggcgga acggttaccg
     3301 tagaccccga ctacgacggc actgcctcag gtgagggtgg cgcgttaagc aacaccatat
     3361 tccagcaggt tgcccatgct agcggaaccg caagtggcga tgtcatcacc ttaaaagaac
     3421 tggctactat taccggctca acacctgccg gcgacgacta taccgacatc ctaaccgtca
     3481 tcggcgctgg caacttctaa gccaagcctc agtaaccatt tatcgaattc tcaaacccct
     3541 catgctttgt tatactgaca ggtaatgcag cacaagattt tggtggagcg aataatttgc
     3601 atcatgctgg ttgcgacaat aagcgtggcc agtttgtgcg ttccatttgg cgctcaggcc
     3661 ggtactgagt caacgcagcc gggagacttg catctcatta cctcgccgct gccaatcctt
     3721 ttaacggtta aacccgggac aaccactact acagatttgc gcataaaaaa tgccggatcc
     3781 acaactgaac gattgcgggt cgacatgctc aaatttggtg ccagtggcga ggagggccaa
     3841 ccggcactga aagatcccga gcctggggat gagcagttta agtgggccag tttttcacaa
     3901 acaattttca acgctgaacc gaatgagtgg aaaaccgtga agatgaccat taaggtacct
     3961 acgacagcag cctatgggta ctactatgcg ataacctttt cacgggccaa caaggaagcg
     4021 aaaactaccg atcagcgagc cgttctgcgc ggggcaaccg ccgttcttgt gctgctagag
     4081 gctacttctc ccaacaccaa gcgcaaagtt cagatggttg atttcgtttc aaacaagggc
     4141 ttatatgagt atctcccagc cagcttcaag gtgcgtttgc ataatatcgg taatattcac
     4201 accgcacccg taggcagcgt gttcatcacc aagggcagta agccaatagg atcgatgagt
     4261 atcaacaaag aacatgggag tattttgccc aatagttatc gtatttatca gactgagtgg
     4321 aaagacggat ttccgcatta tgccgaggcg gttgaaaaca accagactgc catcaataag
     4381 aagggccagc aggaaaagcg gttggtttgg aatctgggag atatcggaaa gatccgcttc
     4441 ggtaagtata ccgccagcgt tgtaatggtg tacgacgatg gcaagcgcga cattcccgtg
     4501 gaagcggctg tctctttctg ggtaatacca tggcgattct tgctgatagt agggttagtg
     4561 gtaacgctga tgttggcggg attatgggct atggtgcggg tagttaggcc aagccgacgc
     4621 gctaagcgcc ggaggtagcg atgaatcgat ccatcgcccg tagtctgttt ttcggctttc
     4681 tgatcattct tggttgttat tggatatggt ctcatccagt tcaagctgaa agttcaggcg
     4741 gggtagtgat ttcgccgcca ttccagcaag tagtggtagg cgatggcaag ggcacggctt
     4801 ttgacgtcac cgtaaccaat cgcacaacta gcgatcaatc aattacggca acaaccgtag
     4861 attttaagtc gctggacgaa tctggtggcg tagcctttct gggctccgaa atcagcgagc
     4921 ttgagcgtaa atacgggctg gctaactggc tgaagcttga tgctgcacca acgattattc
     4981 cagccggtgc atcgaaaaaa attgttgtaa atctggaaaa tcgtgaagat ttaacgccgg
     5041 gcgggcatta cggcgcggtg atttttaaaa ttgaaggaga taacgcttcg gagaactcta
     5101 attcagtgga cgtaaagcaa gttttagctt cgctcatatt tgctaaaaag ctgggcgggg
     5161 aacgctatga tttaaaactt tcaagccttg atccgccacc taaattagcg agcggcgacc
     5221 aaaaagtgaa gcttagattt tataaccccg gtaatgttca tgtggtgccg cgcgggattt
     5281 tgcggttaaa agattcgaaa gggaaggtaa tcactacaag tctaatcaat caggagtcgg
     5341 gcatcttact cccggaatct taccgagtgt atcctgtgat actaaaaaac attcctgtgt
     5401 taccggcggg aaagtacgtg ctggaagctg agtatcgcta tgacgggatt gagcaaaccg
     5461 cgaagctgac aaaagaaatt actattttcg attggcgtta tgcagccttt ttaggagcgg
     5521 tggggagcgt tagtctggta ccccttttgc tgatacgtaa gtttattgcg catcggcgac
     5581 gcaagaaaca caaaggccgg cgcaagcatt acgctgcttt ttaggcccaa aggctcgtca
     5641 gttcatctgt tattgcgctt gatttcatct gttaaaacgg ttatgctgag aaagcataag
     5701 tattatgtga ggccaataaa aaatcccatg cgagccactg ttattttggg gaagtttaag
     5761 cgtaccaata gtatgatgtt ggtagggctg ttttttgctg tagtaagcct taatgcattt
     5821 ttacaaccgc ggactgccga ggctgcaacc ggcataaatc agcaaattaa ttttcaaggc
     5881 aaggtggtta accccgacgg caccaatatt cctaatggta cgtacaacat cgaatttaaa
     5941 atttatcagg atgggaacgg tgttcttggt ggcggagatg aaacactgaa atggacagaa
     6001 tcccgcctgc gcaataacag ccagggcgtg acgataaccg atggcatttt tcaagttaac
     6061 ttaggttcag ttaatacagc actcggaaca gctgtcgatt ttaatcagga taccttgtgg
     6121 ctttcaatta acctcggcaa cactaatgca acctgtacac cgtttagtgg ttgttcaccc
     6181 gacggtgaaa tgaacccgtt tattcgattt acctcagctc catacgcgct taactcgggt
     6241 aagctcagcg gcctcactag ttctcagttt gttcagttag ctcagggcgt tcaagtagat
     6301 gcctcatcgg cttcaagtat tttcattaat aaaaccggtg caagtggcaa tctactggaa
     6361 ttacagagag ctggtaccga tgtattggcg gtagacaaca acggtaccgc gacactccag
     6421 gccgctgctg gcaataacgc cacacttacg gtgaagcagg caagtaccgc cacttccgga
     6481 gacattttta gcgttacggg gagcaatgga acctccaaat ttattcaggt cactagtacg
     6541 gctgctaatc agggatcggt tattgtgcag tcacttggta gtaacaccct aacggtagac
     6601 tcgggatcgg gcaccttggt ggttggtacg actacgacta ccattcaaaa gagtggggca
     6661 gccttaacta ttgacttagc aaccgctgca gcttcaacct tgacgattac caactccaac
     6721 gcctctaata cagccagcct gagcgtggaa ggcgggggca gtttcggcgg aaacgttctg
     6781 gttaaaaccg actcggcctc ggctttccaa gtgcaaacca gcggttcgac aaacttattc
     6841 aacatcgatt caaccaccgg cgacattact tcgaatcatg ggacgctcac ggttaacgga
     6901 gctaataatc caacaaatgt ggtaatttct ggctccccta gcggaggcac attggcggcc
     6961 agtacaacct ttgcgtacag ggtggcggct accgatattt ccgggtcgat tacccaggcg
     7021 gttgctggaa cgccgacgtc atatacttct ggcgctgggc ccaataataa ccaggtagcc
     7081 gtaagctggt cggccggaac aggggcccag agttatagaa tttatcgaac aaccaatggc
     7141 ggcaccaact ggtttgttaa cactgtttct agtgctacca cgtcaatcaa cgataacggt
     7201 acaaacttta cttggtcaac cgcagggaca ccaccaacca ctaatgctac cggtggcgtc
     7261 actctggctg gtggtacccc gcttatattt aacggaaacg gcggtactgt tacgctccag
     7321 ccctccattt cggttactag cagttatggt cttagcctgc ctaccgggca gggcggcgta
     7381 aataccgttt tgttgaacga cggaagcggt aacttgacct gggttggaac agccggttgt
     7441 agcacctgtg ttgcaacg
//
LOCUS       DASVGB010000008         7995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_271302, whole genome shotgun sequence.
ACCESSION   DASVGB010000008 DASVGB010000000
VERSION     DASVGB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271302"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..868)
                     /gene="mreC"
                     /locus_tag="VF272_00185"
     CDS             complement(62..868)
                     /gene="mreC"
                     /locus_tag="VF272_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7259336.1"
                     /translation="MKHKSVNRRLVGFMLVGLLLAIANTVGLLSPVTALASAALKPLQ
                     ASLAGAASGVGGWFNVIGSARELGNENQRLRAEVAALRGQMSRDTEIKAQNDQLRQQL
                     GVGGIRPDRLIAAEVIGYQPDNFRQFLTIGRGSRDGLREGMTVIQQGALIGRLQDVGP
                     STAKVFLIIDPNFRVAALDQNAPNRPTGTIRGQIGNGLIMDKIAQTETITPGDTIVTS
                     GLGGGIEKGLIVGRVQTVSKQANGVFQTAQVTTDIQFNRLEVIYVIARPQ"
     gene            complement(868..1920)
                     /locus_tag="VF272_00190"
     CDS             complement(868..1920)
                     /locus_tag="VF272_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7259337.1"
                     /translation="MGQFSHDIGIDLGTANTLVYVRGQGIVINEPSVVAINTKTNQIL
                     AIGEEAKRMVGKTPASIVATRPLVDGVVSDFEVTEQMLRYFINKVHKQRFKLLARPRV
                     VIGIPSGVTEVERRAVEDAASNSGARQTFLIEEPMAAAIGARLPVQDAAGSMIIDIGG
                     GTSEVAVISLGGIVAARSIRVAGDELSEDIVKYARDEFKLSIGERTAEQIKIAVGSAY
                     PIAGTDQPRNVLMRGRDMVTGLPKQINVTSDQIREALNRSVREIIDAIKVTIEETPPE
                     LVADIMHRGMVLAGGGALLKGLDVLIQRETRMPVHVASDPLTAVARGTGLVLEDLDSL
                     KDILLPNQSKSASRRY"
     gene            complement(2176..2667)
                     /locus_tag="VF272_00195"
     CDS             complement(2176..2667)
                     /locus_tag="VF272_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259338.1"
                     /translation="MASSADNPLHVRNQLVQQRLQFWSRWLLYTLGCLIGMAVLPVWH
                     IPVVPTLLMLAAMFCLTVIAAEMIGEWWEVAAVFVFVAVAGQVLLYGGFSTRSVFVAA
                     WSLLLAAACLGCRELNIKRRNKRFHGRGMKGFRNQLFLLGPARPPLKDLLRQVRRFGR
                     LRR"
     gene            complement(2739..3980)
                     /locus_tag="VF272_00200"
     CDS             complement(2739..3980)
                     /locus_tag="VF272_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX7259339.1"
                     /translation="MRLSNLFTKTRREAPKDETATNAQLLIRAGFIHKDSAGVYVFLP
                     LGLCVLNKIIAIIREEINALGGQELAMSSLQRKELWERTDRWDDEQVDVWFKTRYKNN
                     TDVGLAWSHEEPLTELMREHISSYRDLPVYAYQFQTKLRNETRAKSGIMRTREFIMKD
                     LYSFSRSEEEHKAFYKRAQDTYATIFDRLGLGEWTYLTFASGGAFAEFSHEFQTITDA
                     GEDSIYVDHAKRIAINEEVYSDTLINDLGLTKDTLEKTKAAEVANIFNLGTSKSQPLG
                     LVFTDEDGTQKPVVMGSYGIGPARVMGVVVERYADDRGMVWPEAIAPANVHIVQIGDA
                     ADTVRAAEKVYADLQKQGVEVLYDDRPDVTAGAKFADADLMGCPVRLTISQKTLEHNS
                     VEIKKRTAAASKLAPIDDIKI"
     gene            complement(3992..4714)
                     /locus_tag="VF272_00205"
     CDS             complement(3992..4714)
                     /locus_tag="VF272_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX7259340.1"
                     /translation="MASYAASIRSVPWTPSQGLLLFVLPWIILPVGLIINVSIAANFI
                     PSLRGFMNALGSGDPAAAFALVLVDAVGSMALIWYFLRKYKVGLSSLGVRSFSIGKAL
                     WYLVLLFVSFGVLITAADILVKLIYPGFNPAEPQTNEFISSPSSLSLWALVLIPPIIE
                     EAVFRGFLFPAFAKRYGVVIGAMISSLLFAVAHLQLNVSIYTFILGLLLCFLYVRLGS
                     IIPGIGIHMLNNYLAYMALNQQ"
     gene            complement(4716..5795)
                     /locus_tag="VF272_00210"
     CDS             complement(4716..5795)
                     /locus_tag="VF272_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7259341.1"
                     /translation="MIIGIILLVILIFGILVFVHEFGHFIMAKRNGVDVEEFGFGFPP
                     RLVGVKYGQTVYSINLIPLGGFVKMKGEAIQESTPGTFGAASFLAKSKILLAGVTMNA
                     LLAYAVLLWLCLTGLPPVISGQYSFGTPTYAQPKQLMAVAVDPASPAARAGLERGDIL
                     LSANGTPLKEERDLQTFTKEHAGQKVTLQISHDGKTRTINPTLRPPSAASGFLGVTPF
                     QTYQLRYDFLSALVTAGGISLQLIYHTFIAFAGLIAGLFTQGQVSENVAGPVGIVVLL
                     KNIVDLGLPYILLFVASISVSLAVINILPLPALDGGRYAMVVAQRLTGKSLSQKAETA
                     VHAAGFAALILLMIVITVFDIRRLG"
     gene            complement(5825..6385)
                     /gene="frr"
                     /locus_tag="VF272_00215"
     CDS             complement(5825..6385)
                     /gene="frr"
                     /locus_tag="VF272_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7259342.1"
                     /translation="MVPQIVKQADPRMEKAVQYLEDELRSLRVGRAQTALVENLTVEQ
                     YGQTMLLKAVATITTPDAHTITISPWDKSMVASIEKMLRENQALGLNPSSDGNVVRLN
                     VPPMTEDRRREIVKELGVKVENSHISLRQIRHDVLGEVKRMEKSGEATQDDMRFAEQE
                     LNKKIDYYKKKADELAKAKEAEIMQV"
     gene            complement(6385..6978)
                     /gene="tsf"
                     /locus_tag="VF272_00220"
     CDS             complement(6385..6978)
                     /gene="tsf"
                     /locus_tag="VF272_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7259343.1"
                     /translation="MAEVTIEAIKNLRETTGAGMMDAKKALVEAKGDVEAAVAALRKK
                     GMARAAKRADREVGAGLVSTYVHGDKIGVLVEVNCETDFVARTDDFKTFVRDVAMHIA
                     ASAPEYLNAESVPADVLAKEREIFAEEVKGKPENVVEKIVEGKLAKYYQTVCLINQPF
                     VKDPDKSIEEYTTEVAAKVGEKVVIRRYARMVLGESL"
     gene            complement(7007..7747)
                     /gene="rpsB"
                     /locus_tag="VF272_00225"
     CDS             complement(7007..7747)
                     /gene="rpsB"
                     /locus_tag="VF272_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7259344.1"
                     /translation="MATPVKELLEAGSHFGHQTRRWNPKMGKFIFGVRGGVHIIDLTK
                     TVNYLNDAAAFVERVAEDGGKILFVGTKRQAVAIVRDEAGKAGQPYVVERWLGGMLTN
                     FRTIQDRIKRLKQIEEGLESGDYATKYNKKELLDLTNEKEALERIFGGIKDMRELPKA
                     VFVVDAVRDSIAIAEARNLNIPLVVIVDTNADPDVATYPIPANDDAIKSIRVISHAIA
                     EAADKGAQAFVRKKPAEAVAAPEAAEKA"
BASE COUNT         1986 a         2208 c         1916 g         1885 t
ORIGIN      
        1 ccaaaccacg caacctgcac gcaagcaacc aggattatca gcaatgtccc ccacgccagc
       61 ttcactgcgg cctggcaatc acataaataa cttccagccg attgaattga atgtcggtgg
      121 taacttgggc ggtctgaaat accccgttag cctgtttgct tacggtttgg actcgtccga
      181 caatcaaccc tttttcaata ccgccgccaa gaccgctggt gacgatggta tcgcccggcg
      241 taatcgtttc agtttgggcg atcttgtcca taatcaaccc attgccaatc tgtccgcgaa
      301 tggttccagt gggacgattg ggcgcatttt gatcaagcgc cgcaacccgg aaattcggat
      361 caattatcag aaagacttta gcagtactcg gaccaacatc ctgaaggcgg ccaattagcg
      421 caccttgctg aatcacggtc ataccctctc ttaaaccgtc gcggctacca cggccaatgg
      481 ttaagaactg gcgaaagtta tcgggctgat agccaattac ctcagcggca atcaggcggt
      541 cggggcggat accgccgaca ccgagctgct ggcgtagctg atcgttctga gctttgatct
      601 cagtatcgcg gctcatttga ccgcgcaagg cggcaacttc ggcccgcaag cgctgatttt
      661 cattccctag ttccctggcc gaaccaatta cattaaacca accgccaacc ccgctagcgg
      721 cgccagctaa gctagcctgc agcggtttaa gcgccgctga agccagcgca gtaaccggtg
      781 ataacagccc gactgtattg gcaatcgcca gcaagagtcc taccagcata aatcctacca
      841 atcgccggtt aacactttta tgcttcatta atatctccgt gaggcagact tagactggtt
      901 gggcagaaga atatctttca atgaatcaag gtcttccaga accagccccg taccgcgcgc
      961 cactgcggta agcggatcgc tggcaacatg caccggcatt cgggtttcac gctgaatcag
     1021 cacatccagc cccttcagaa gagcgccgcc gcctgccagc accataccgc gatgcataat
     1081 atcggctacc agctctggcg gcgtttcctc aatggtaact tttatggcat caataatttc
     1141 gcgaacgctc cggtttaaag cctcgcgaat ttgatcggag gtaacattaa tttgtttggg
     1201 caaaccggtt accatgtcgc gcccgcgcat taacacattc cgcggctgat cggttccagc
     1261 gatcggatac gccgaaccaa ccgcaatttt aatctgttcg gcggtgcgtt cgccgatact
     1321 caatttaaac tcatcgcgcg catactttac gatatcttcg ctgagctcgt cgccggccac
     1381 ccgaatacta cgggctgcta cgatcccgcc caaggaaata accgcaactt cgctggttcc
     1441 gccgccaata tcaataatca ttgaaccggc cgcatcctgg accggcaagc gagccccaat
     1501 agccgcagcc atcggctctt caattaaaaa ggtttggcgg gcaccggaat tgctggccgc
     1561 gtcttctacc gcccgacgtt caacctcggt aactcccgat ggaataccaa taactacccg
     1621 tggtcgtgcc agcagtttaa accgttgctt gtgcaccttg ttaataaaat agcgcagcat
     1681 ttgctcggta acttcaaagt cacttaccac tccatcaacc aacgggcggg tagcaacaat
     1741 acttgccggg gttttaccaa ccatccgttt ggcttcctcg ccaatggcta ggatttgatt
     1801 tgttttggtg ttaatggcga caacgctggg ctcattgata acgatgccct gtccgcgaac
     1861 gtataccaaa gtattggcgg tacctaggtc gataccgata tcatgggaaa actgccccaa
     1921 caactgatca aacacggata agctcaaaca ttaccatctt ggatctagtt tacaggagtg
     1981 gtgaggctcc gccaaggtca caatatgtgt tcaggactga acccaggggc agtgccagat
     2041 agggcgaaca agagatgtac tcaaaatact ctaagccggc ctcaaaatgc agccatcatt
     2101 tttccccaaa gccatccaaa aatgtagctt agagcgtttg atcaaaataa gtgcggtcag
     2161 tccggccctg gcgcgctagc gccggagccg accaaaacga cggacctgcc gcaggaggtc
     2221 cttgagaggc gggcgggcgg gaccaaggag aaacagctgg ttccggaagc ccttcatgcc
     2281 ccggccgtga aagcgcttgt tacggcgctt gatgttcagc tctcgacatc cgaggcaggc
     2341 agctgcaagc agcagcgacc aggccgccac gaagacggac cgggttgaaa acccaccgta
     2401 aagcaagacc tggcccgcca cggcgacgaa tacgaagacc gctgcaacct cccaccactc
     2461 gccgatcatc tcggcagcga tgactgtgag gcagaacatc gcggccagca tcagcagcgt
     2521 cgggactacg gggatatgcc agacaggcag cacggccatg ccgatcaagc agcccagcgt
     2581 gtacagcagc cagcgcgacc agaactgcag tcgctgctga acgagctggt tgcgtacgtg
     2641 cagaggattg tcggcagaag aagccatacg tctccttttt gtcgaggtat cccacgatta
     2701 tgggctaaca gtttatacca taattgcttg ttgctttgct agatcttgat gtcgtcaatt
     2761 ggagcaagct ttgatgcggc cgcagtccgc tttttgattt caacggaatt gtgctccagg
     2821 gttttttgac taatggtcag ccggaccgga cagcccatca gatcagcgtc ggcaaacttg
     2881 gcaccggctg tgacatcggg acgatcatca tataacacct ccacgccctg cttctgtaaa
     2941 tccgcataaa ctttttccgc cgcccgaacc gtatcggcgg catcaccaat ttgaactatg
     3001 tgaacgtttg ccggcgcaat tgcttctggc caaaccatgc cgcgatcgtc ggcgtagcgc
     3061 tctaccacca cacccataac tctggccggc ccaatcccat agctgcccat tactaccggc
     3121 ttctgtgtac cgtcctcgtc cgtgaacacc agtcccaatg gctggctttt gctcgtgcca
     3181 agattaaaaa tattggcaac ttcagccgcc ttggtctttt ccaatgtgtc cttggtcaac
     3241 cccaagtcat tgattaacgt gtcgctgtag acttcttcat taatggcaat ccgtttggca
     3301 tgatccacat aaatactatc ttctccggca tcggtaatgg tttgaaactc atggctgaat
     3361 tcagcaaacg cccctccgga cgcaaaggtc agataggtcc actcccccaa ccccaaccgg
     3421 tcaaatattg tggcataggt gtcttgagcg cgcttgtaaa aagccttgtg ctcttcttct
     3481 gaacggctaa aggagtacag gtctttcata ataaactcgc gggtacgcat aatgccggac
     3541 ttggcccgag tttcgttgcg cagcttggtc tgaaactgat aggcatatac cggcaaatcg
     3601 cggtaactcg aaatgtgttc gcgcatgagc tcagtgagcg gctcttcgtg gctccatgcc
     3661 agaccaacat cggtattgtt tttatagcgc gtcttaaacc agacatcgac ctgctcatcg
     3721 tcccatcggt cggtacgctc ccagagctcc ttgcgctgca aactgctcat ggcaagctct
     3781 tgaccaccca atgcgttgat ttcttctcga ataatagcaa tgattttatt gagtacgcac
     3841 agacccagcg gcaaaaagac atacacgccg gccgaatctt tatggatgaa gccggcacga
     3901 ataagcagct gcgcgttggt agcggtttca tcttttggtg cctcacggcg ggttttggtg
     3961 aaaaggttgg ataaacgcat cgtattactg gttattgttg gttaagggcc atgtaggcaa
     4021 ggtagttatt gagcatatga ataccgatgc caggaataat tgaacccagc cggacgtaca
     4081 aaaaacataa taacaggcca aggatgaagg tgtatatgct gacgttgagt tgcaggtggg
     4141 ctaccgcaaa cagcaaactt gatatcatgg ccccgattac tactccgtag cgcttggcaa
     4201 atgccgggaa cagaaagccg cggaaaaccg cctcttcaat aattggtggt atcaacacca
     4261 aggcccataa gctgagactc gatggactac taataaattc gttggtttgc ggttcggcgg
     4321 gattaaagcc gggataaatc agcttcacca agatatcagc ggcggttata agtacgccaa
     4381 aactaacaaa cagcaatact agataccaca gggccttgcc aattgaaaag cttctcacgc
     4441 ccagcgacga caagccaacc ttatacttgc gcaagaagta ccaaatcaat gccatggaac
     4501 cgaccgcgtc aaccagtact agggcaaaag cggcagctgg atcacccgat ccaagagcat
     4561 tcataaaacc tcgaaggctg ggtataaagt tcgccgcaat acttacatta atgatcaacc
     4621 ctaccggcag aattatccaa ggcaatacaa acagcaacag tccctggctg ggagtccaag
     4681 gaaccgatcg aatagaggcg gcgtatgacg ccatcttagc caagccgccg gatgtcgaac
     4741 accgtaatta cgatcattaa tagaatgagt gctgcaaacc cggcagcgtg aaccgccgtc
     4801 tcggctttct ggcttagcga cttgccagta agccgctggg caaccaccat ggcataccgg
     4861 ccgccatcaa gtgccggcag cggcaaaata ttaataaccg ccaacgacac cgatattgag
     4921 gcgacaaata gtaagatata gggcaagcca aggtcgacga tatttttcaa taacacgacg
     4981 atccctaccg gaccagcaac attctcgctt acctgaccct gggtaaacaa gccggcaatg
     5041 agaccggcaa acgcaataaa ggtgtgataa atcagctgta aactaatccc cccagcggtt
     5101 accagggcac tcagaaaatc atagcgtagc tgataggttt gaaatggcgt cactccaagg
     5161 aacccgctgg cagcacttgg cggccgtaat gttgggttaa tggtgcgtgt tttgccatca
     5221 tgggaaattt gcaaagttac cttttggccg gcgtgctcct tggtaaacgt ttgcagatca
     5281 cgctcctcct tcagtggtgt accattagca ctgagtagga tatctccgcg ctccaatcca
     5341 gcccgagccg ccggagaagc cgggtcaacc gccaccgcca taagctgctt cggttgggcg
     5401 taggttggcg taccaaaact atattggccg ctaatgaccg gcggaagtcc ggttaagcac
     5461 agccacagga gcacagcata tgccagaagc gcgttcatag ttacccctgc cagtagaatc
     5521 ttggacttcg ccagaaaact ggccgcccca aaggtaccag gcgtcgattc ctgaatcgcc
     5581 tcacccttca ttttgacaaa gccgccaagc gggatcaggt taatggaata gacagtctgg
     5641 ccatatttca cgcccaccaa gcgtggggga aagccgaacc cgaactcctc aacatcaacc
     5701 ccattacgct tcgccatgat gaaatggcca aactcgtgca cgaacaccag aataccaaaa
     5761 atcagaataa cgagcagaat aatgccgatg atcattgctc cattgtacta tactggccgg
     5821 ttgattagac ctgcatgatt tcggcttctt tagccttggc caactcatcc gcctttttct
     5881 tataataatc aatcttttta ttaagctctt gctcggcaaa acgcatgtcg tcctgagtgg
     5941 cctcgccgga cttctccatc cgcttaacct cgcccagtac gtcatgacgg atctggcgca
     6001 aggaaatatg gctattttca accttaacgc ccagttcttt gacaatctct cggcggcgat
     6061 cttcagtcat tggcggaacg ttcaagcgta caacgttgcc atcgcttgag ggattaagac
     6121 ccagtgcctg attctcgcgg agcatctttt caattgatgc aaccattgat ttatcccacg
     6181 ggctgatggt aatggtatgg gcgtccggcg tcgtgatggt agctaccgct ttaagcagca
     6241 ttgtctggcc gtattgctct acagttaggt tttctaccag cgccgtctgc gcccggccta
     6301 cgcgtaagct tcgcagctca tcttccaggt actgaaccgc tttctccatg cggggatcgg
     6361 cttgtttgac gatttgcggt accattatag cgattctccc aagaccatac gggcatagcg
     6421 gcgaataaca accttctccc ccaccttagc agcaacttca gtcgtatatt cttcaattga
     6481 tttatcggga tctttgacaa agggctgatt gatcaaacaa acggtttgat aatatttggc
     6541 gagcttgccc tcgactattt tttcgacaac gttctccggc tttcccttta cctcttcagc
     6601 aaaaatctcg cgctcttttg caagcacgtc agcggggacc gattcggcat tcaagtattc
     6661 tggagcgctg gcagcaatat gcatggcaac atcgcggaca aatgtcttaa aatcgtcggt
     6721 acgagccaca aaatcggttt cgcagttcac ttcaaccaag acgccaattt tatcaccgtg
     6781 aacgtaggta gaaaccaggc cggcaccgac ctcgcgatcg gcacgcttgg cggctcgagc
     6841 cattcctttt ttgcgcaagg ctgcaaccgc cgcctcaaca tctcccttcg cctctacaag
     6901 tgctttttta gcatccatca tgccggcacc ggtagtttca cgtaagtttt taatagcttc
     6961 aatagttact tctgccatga atactcctca attcttactt gtttgcttag gccttttcgg
     7021 cggcttcagg tgcagcaacc gcttcggccg gcttcttacg cacaaatgct tgggcgcctt
     7081 tatcggcggc ttcggcaatg gcgtggctga tcacacgaat cgatttgatg gcgtcatcgt
     7141 tagctggaat cgggtaagtc gctacatctg gatcggcgtt ggtgtcaaca attaccacca
     7201 atggaatgtt taggttgcga gcttcggcaa tcgcaattga atcgcgtacc gcatcaacca
     7261 caaacactgc cttgggcagc tcgcgcatat cttttatgcc gccaaaaatg cgctcaagag
     7321 cttctttttc gttggttaag tcgaggagct ctttcttgtt gtacttggtg gcataatcgc
     7381 cgctttccag gccttcttca atttgcttta agcgcttaat ccggtcttga atggtacgaa
     7441 agttggtaag catgccgccc agccagcgtt caaccacgta gggctgcccg gctttccccg
     7501 cctcgtcgcg aacaatcgcc acggcctggc gcttggtgcc aacaaacaag atcttgccgc
     7561 catcttcggc aactctttca acaaatgccg cagcatcgtt aagatagtta accgtcttgg
     7621 ttaggtcaat aatatgaacg ccgccgcgta cgccgaaaat gaacttgccc attttcgggt
     7681 tccagcgacg ggtttgatgg ccgaaatgcg aaccggcctc taatagctcc tttacgggag
     7741 ttgccatgtg attgttatcc ttttacgttg tctgcttggc cgctcgttat agttagggag
     7801 cggaggattt tggtaagcaa actgtgtcat taattagttc taacgggagt aatagtagca
     7861 gaattgcgcc cgaccaacaa gcctcttcta gctggtatta tacaaacatg gatgaaaaac
     7921 aacggttgcg tcttatttat agggaaaagt tagcggcttt atccggcact gaacggcagg
     7981 acaaaagcca tacca
//
LOCUS       DASVGB010000009         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_270773, whole genome shotgun sequence.
ACCESSION   DASVGB010000009 DASVGB010000000
VERSION     DASVGB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270773"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1203
                     /locus_tag="VF272_00230"
     CDS             292..1203
                     /locus_tag="VF272_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7259345.1"
                     /translation="MFERFTEVARHVVVLAQEEARRFKHGYIGDEHILLGLLGEEEGL
                     AAQVLGSFDITIERSRAQVVKLVGTGEFASPEIIPFTPRAKKLFELSFREAISLGHGY
                     IGTEHILLGLVRQGDGIAARMLLDFDADADRIRSEVMQQIGNINYILEEVADPEGRFG
                     RGTRAVVRRARNTAWDLNHLDIKPIHLLIALAQLHEGVIGETFKALQINPKNLRDQAA
                     AALPVPLAPSPEKILLHPASRKVLDLAVEQANMMDRHEIRPHHLMLGLLKAADPVTTD
                     ILVGIGIDTHRAEAAVAMCILKANARS"
     gene            1387..1653
                     /locus_tag="VF272_00235"
     CDS             1387..1653
                     /locus_tag="VF272_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259346.1"
                     /translation="MNRYSSGSPKHNRPGEIVVSLVASGVILAISLATLTAWAYLGSV
                     LLLGSGIIFLLIGIGATFHQWAQLRLHFNPVPPVSDLQAEPSPS"
     gene            1840..2160
                     /locus_tag="VF272_00240"
     CDS             1840..2160
                     /locus_tag="VF272_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7259347.1"
                     /translation="MDNPAITQKTSLETLKLLGDFWTLRIIDALKDGELRYCGVQRQA
                     GGINPVTLSSRLKKLEQAGIIRRQDCAQDKISVSYRLTELGRGTLPVLNAINQFSDKL
                     RPAV"
BASE COUNT          509 a          607 c          544 g          505 t
ORIGIN      
        1 catggattca tacgttgatt caagattatg ccgctttata tgaggtataa agaaactaat
       61 ttcgcgcgga actttcatac atggcccctt tcaggcggct aaggggcact taaccagagc
      121 agttgattag atttattgta gcatgaatta ctataattag ctaattttgt tacaaatctg
      181 tcaagctctc agagcggtgc attgccttga tcagtacacg tattaatgat ctaatagtct
      241 gacgggacta ccagtctcta tcgtgtacac ccgcaatcga acgagggcac aatgttcgaa
      301 cgctttaccg aagttgcccg tcacgtggtc gtactcgcac aagaggaggc ccgcaggttc
      361 aaacacggct acatcgggga tgaacacatc ctgcttggcc ttctgggaga agaagaaggc
      421 ctggcggcgc aggttctggg aagtttcgac atcaccattg agcgctctcg tgcccaggtg
      481 gtcaagcttg tcggaaccgg agagttcgct tcacccgaga taatcccgtt cacgccgcgg
      541 gccaagaagc tgtttgagct gtcgtttcgc gaggcgatca gcctcggaca cggctacatc
      601 ggcactgagc acatcctgct cggtctagtc cgccagggtg acggcattgc tgcccgcatg
      661 ctgctcgact tcgacgctga tgccgacagg atccgcagcg aggtcatgca gcaaatcggc
      721 aacatcaact acattctcga ggaggtagcc gaccccgaag gccgctttgg tcgcgggacg
      781 agggctgtag ttagaagagc ccgcaatacg gcctgggatc tcaaccacct cgacatcaag
      841 cccatacacc tgctgatcgc gctggcgcag cttcatgagg gcgtgattgg cgagaccttc
      901 aaggccctgc agatcaaccc caagaacctc cgcgaccagg cggcggctgc cctaccggtc
      961 ccgcttgcac catcaccgga gaagattctg ctccatcccg caagcaggaa agtcctcgac
     1021 ctggcggtcg agcaggcgaa catgatggat cgtcatgaga tcagacctca ccacctcatg
     1081 ttgggactgc tcaaagcagc ggatcccgtg acgaccgaca tccttgtcgg gatcggcatc
     1141 gacacccatc gagccgaagc tgccgttgcg atgtgcatcc tcaaggcgaa cgcaagatcc
     1201 tgaagcatcc catactctat tctaggcacg gcaaccccaa tgggcaggca ttctagatat
     1261 catcctgccc caaccgcact tatcaacctc aaaagtattg cgattttgct tgacttaagt
     1321 tatttttaat tgtgtgccac acccgtagca gctgaaattt cagtgcaacc cacaggaagg
     1381 acggacatga ataggtacag ttccggctcg ccgaagcaca atcgcccagg agagatcgtt
     1441 gtctcgttgg ttgcttcagg tgtcatcttg gcaatcagcc tagccactct taccgcgtgg
     1501 gcgtatctcg gaagcgtgct cctgctgggc agcgggatca tctttctcct cattggcatc
     1561 ggcgccacat tccatcagtg ggcccagctt cggctacatt tcaatccggt tccgccggtc
     1621 agcgatctgc aagccgaacc cagcccttcc taaaccccgc tggggcggct aacgaatctc
     1681 gcatacgcgt gcggcttcgg tcgcaccggc gggtttcgtt ttttatgtgc ggaggctttt
     1741 cccagccgca accgtttata cggttgcttc atgaaagctt caccccgttg tctccctctt
     1801 ctggatatgt tactataaaa attatagcaa gtaaatttca tggacaaccc tgcaattacc
     1861 caaaaaacat ccctcgaaac cctaaaatta ctgggtgatt tttggacttt gcggattatt
     1921 gatgcgctta aggacgggga attgcgttac tgtggcgtac agcgccaagc cggcggtatt
     1981 aatccggtta cactttcatc tcgtttgaag aaactggaac aagcaggtat tattcggcgc
     2041 caagactgtg ctcaagacaa aatctcggta agttatcggc ttaccgaact gggccgtggc
     2101 acccttccag ttcttaacgc tataaaccag ttttccgaca agcttagacc cgcggtataa
     2161 tacca
//
LOCUS       DASVGB010000010         6582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1265751, whole genome shotgun sequence.
ACCESSION   DASVGB010000010 DASVGB010000000
VERSION     DASVGB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265751"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3484)
                     /gene="rpoC"
                     /locus_tag="VF272_00245"
     CDS             complement(<1..3484)
                     /gene="rpoC"
                     /locus_tag="VF272_00245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7259348.1"
                     /translation="MEPLMLDQTASEFSAVRLSLASPQQILEWSHGEVTKPETINYRT
                     QKPEKDGLFCERIFGPTKDWECYCGKYKRIRYKDVVCDKCGVRVTRSIVRRERMGHIS
                     LAVPVTHIWFLRGTPSSIGLVLNMSIRDLERVVYFANYVVSEVDDERRKAALVELEAD
                     YKSRREEIEKDHTKQAADKDAKVKEVAASQSRELSDLEVTYQNAKEELDGLARLTLLT
                     ESKYRDLNMKFGDVFKAEIGAEAIYNLLTSIDVDQLVGELTKEAEEASGQKQKKILKR
                     LKTLEGMRGAGIRPEWTVLKELPVIPPDLRPMVQLAGGRFAASDLNDLYRRVINRNNR
                     LKRLIELDAPEVIRRNEKRMLQEAVDALIDNNARRERAVSSTGTRRKLKSISDMLKGK
                     QGRFRQNLLGKRVDYSGRSVIVSGPTLKLNQCGLPKMMALELFKPFVMAKLIEEGHAH
                     NVKSASRMIERARAEVWDALEYIITDKHVLLNRAPTLHRLGIQAFKPKLIEGKAIQLH
                     PLVCSAFNADFDGDQMAVHVPLSAAAQHEAATIMLSSNNLLRPGDGSAVVNPSQDMVL
                     GCYYLTYDKYAGEDPKKHFSGTDEAIYAYESAVVALQAPITIPGTDGRMTTTVGRILF
                     NEVLPEEIGFRNETMTKKKLQALVGEVFVKCGNDATAKVVDSIKDLGFFHATSSGLSI
                     GIDDFVVPADKEELIAQGEQQVVELSKQFEMGLITDEERYRRTIEAWQKTNEKIKAEL
                     EADLAHSNATIGLFVESGARGDLSQVNQISGMLGLVNNPTGRIIELPIRSNYKEGFSM
                     LEYFSSTHGARKGLTDTALKTAESGYLTRRLVDVAQDVVVTNEDCRDTEGNVVSATES
                     VAIGERFTARLAGRTAAQAVKDGSTTIVKKGELISDQAAEQIMAAGIEEVRLRSVLSC
                     KIQWGVCQKCYGTDLARGTIVKLGEPVGVIAAQSIGEPGTQLTMRTFHKGVAATGADI
                     TQGLPRVEEIFEARSPKGQAVLAELDGIVAIKQAGGKTNVRVTPANVKVTEYALGDRI
                     PEVKSGDHVEIGDILAADGKGKKLIKAKIEGLIEVTKDKILLTHAGGAFKDHVIPAYQ
                     EIEVENGDLVVRGQRLTEGSINLQDMLTLRGELAVQRYIVGEVQSIYASQGQTIADKH
                     IEIIIRQMFSRVQVE"
     gene            complement(3485..>6582)
                     /locus_tag="VF272_00250"
     CDS             complement(3485..>6582)
                     /locus_tag="VF272_00250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7259349.1"
                     /translation="NTGSALLFGAKVIPDRGAWLEFDTAPNGVISVKIDRKRKLPVTT
                     LLRSFGYAEDKEIKALFAKVDTGEIKFIDETLDKDATSNFSEALMEVYRRIRPGDLAT
                     VENSKSLIEGMFFDPKRYDFSKVGRYKLNKRLAIDIPNTYENRIFRREDLVAIIAEII
                     RQNNDPNAQPEDIDNLANRRLRMVGELIQQKFRVGLLRMERNVKDRMSVYDRETVTPG
                     QLINVRPVVASVKEFFASSQMSQFMDQTNPLAEIAHKRRITAMGAGGLSRERAGFEVR
                     DVHRSHYGRLCPIETPEGPNIGLVSNLSTYARVNEYGFIETPYRTVINKIAPNDAIGY
                     IARTTIKDEKGTVIVKPGDTITAAHAKKLAALKLDTVPVKAKVTDEVRFMDAADEADA
                     VITEAGTKVGPDNYFIEPRVPVRRGGEAEEEDANNVEHMDVSTRQIVSVSASLIPFIE
                     HDDPKRALMGSNMQKQAVALVKPQAPIVGTGMEGIVAHDSAQVVVAEANGMVSQASAA
                     EITVQYEGKNLDAVTYPLLNFVRSNDGASIHQRTIVRSGQKVKKGEILADGMSTENGE
                     LALGQNVLVAFMSWGGYNFEDAIILSRRLVQDDRYSSIHIETHQIDVRDTKLGPEVVT
                     RDIPNVSEDSLKDLDETGIVRIGAEVTAGDILVGKITPKGEQELSSEEKLLRAIFGEK
                     ARDVKDTSLRMPNGERGKVVGVKIFSRDNGDELPAGVIEQYYVSVAQMRKVSVGDKMA
                     GRHGNKGVIARILPIEDMPHLEDGTPVDMILNPLGVGARMNLGQVFETHMGWAAQALG
                     YTVASPVFNGITIEKIKSELKRAGLPEDGKSQLFDGRTGEAFQERTTVGYKYMLKLNH
                     MVDDKIHARSTGPYAMVTQQPLGGKAQRGGQRFGEMEVWALEAYGAANTLQEILTIKS
                     DDVVGRSKAYEAIIKSEEIRGPRIPESFNVLVKELQSLSLSVDLIREESDVEVDAEDV
                     LTKNIETEVDELGTEPLIQGDDAVADMPLVDLAGGQEDALQAEAEESAGSAREEQEQE
                     A"
BASE COUNT         1526 a         1815 c         1532 g         1709 t
ORIGIN      
        1 cctcaacctg tacccgtgaa aacatctggc gaataatgat ctcaatatgc ttatcggcaa
       61 tagtctgtcc ctgagaagca taaatcgatt gaacctcgcc tacgatgtag cgttgaaccg
      121 caagctcgcc gcgcaaagta agcatatctt gcaggttgat agatccctcg gtaaggcgtt
      181 ggccacgtac gaccagatcg ccgttttcaa cttcaatttc ctggtaggca gggattacgt
      241 gatctttaaa agctccaccg gcatgggtta gcaagatttt atctttagtt acctcaatca
      301 gaccttcaat cttggccttg atcagcttct tgcccttacc gtcagccgct aaaatatcgc
      361 cgatttcaac gtgatcgccg ctttttactt cggggatacg gtcgcctaag gcatactccg
      421 taacttttac gttagccggg gttacccgca cattggtttt accgccagcc tgcttgattg
      481 caacgatgcc atcaagctca gccaggaccg cttggccctt cgggctacgg gcttcaaaaa
      541 tttcctcaac ccgcggcaaa ccttgggtga tgtcggcacc ggtagcggcc acgcctttat
      601 ggaaggtacg catggttagc tgcgttccgg gctcgccaat tgactgggca gcaataacgc
      661 ctacaggctc gccaagctta acaatcgttc cacgtgctag atcggtgccg taacatttct
      721 ggcagacacc ccactgaatc ttacaggaaa gcacgctgcg caaccggacc tcttcaatgc
      781 ctgccgccat gatttgttcg gctgcttgat cggaaattag ctcacctttc ttaacaatag
      841 tggtgcttcc gtccttaacg gcctgcgcgg cggtacggcc agcgaggcgg gcggtgaagc
      901 gctcccctat cgcaaccgac tctgttgcgc taacaacatt gccttcggtg tcgcggcagt
      961 cttcattggt caccactacg tcttgtgcca cgtctaccag acggcgggtc aaatagcccg
     1021 actcagcagt ctttaacgcg gtatcggtta atcccttacg ggcaccgtgg gtggagctaa
     1081 aatactccag catagaaaag ccttctttgt agtttgaacg aattggcagc tcaatgatgc
     1141 ggccggtcgg gttgtttacg agccctaaca tcccggagat ctggtttacc tggcttaggt
     1201 cgccgcgggc acctgattct acaaacaggc cgattgtagc attgctatgg gccagatcgg
     1261 cttccagctc ggctttaatc ttttcattgg tcttctgcca ggcctcgata gtccggcgat
     1321 agcgctcttc gtcggtaatc aaacccattt caaactgttt actgagttcc acaacctgct
     1381 gctcgccctg cgcgatcagt tcttctttgt ctgcagggac cacgaaatca tcaataccaa
     1441 tcgataaacc ggatgatgtt gcatgaaaga atccaagatc cttaatgctg tctacaacct
     1501 tagcggttgc atcattgcca cattttacga acacttcgcc aactagagct tgcagcttct
     1561 ttttggtcat agtttcgtta cgaaaaccga tctcttccgg caaaacttca ttaaacagaa
     1621 tgcggccaac ggttgtagtc atgcggccgt cagtcccggg aattgtaatc ggagcctgca
     1681 aggcaaccac ggctgactcg tacgcatata ttgcctcgtc ggtaccggag aagtgcttct
     1741 tcgggtcttc gccagcatac ttgtcgtacg tgaggtagta acagcccaat accatatctt
     1801 ggcttgggtt caccaccgcc gaaccatcgc cagggcgaag taagttgttc gaagaaagca
     1861 taatcgtggc tgcttcatgt tgagcggcgg cactaagtgg tacatgaact gccatttggt
     1921 caccatcgaa gtcagcatta aaggctgaac ataccagcgg gtgcagttgg attgccttac
     1981 cttcaatcag tttaggcttg aatgcctgaa tacctaaacg gtggagcgtc ggtgcgcggt
     2041 taagcagtac gtgcttgtcg gtaataatgt actccaaagc gtcccaaacc tcagcccgtg
     2101 cccgctcaat catacggctg gcgcttttaa cgttatgagc gtggccctct tcgattaatt
     2161 tagccataac aaacggctta aatagctcta aggccatcat ttttggcagg ccgcactggt
     2221 ttaatttcaa tgtaggaccg gatacgatca ccgatcggcc ggagtaatca acgcgcttgc
     2281 ccagcaggtt ctggcgaaag cgtccttgct tgcctttcag catgtcgctg atgcttttta
     2341 gcttgcggcg cgtgccggta gagctgaccg cacgttcgcg gcgggcatta ttatcgatta
     2401 acgcatcaac cgcttcctgg agcatacgct tttcattgcg gcgaataact tccggcgcat
     2461 caagctcaat taatcgtttt aaccggttgt tgcggttaat tacgcggcga tataaatcgt
     2521 ttaagtctga tgcggcgaac cggcctccag caagctgaac catcggacgc aaatccggcg
     2581 gaataaccgg cagctccttg aggacggtcc attccggacg aataccggca ccacgcatgc
     2641 cttcaagagt tttaagacgc ttcaaaattt tcttttgctt ctggcctgaa gcttcctcgg
     2701 cttctttggt caactctcca accaactgat caacgtcgat tgaagtcagc aagttgtaaa
     2761 tcgcttcggc gccgatttca gctttgaaga catcgccaaa tttcatatta agatcgcggt
     2821 atttcgactc ggtcaagaga gtcaaccgag ctaaaccatc aagttcttct tttgcatttt
     2881 gataggtaac ttcaagatcg ctgagttcac ggctttgcga agcagcaacc tccttaacct
     2941 tggcatcctt atcggctgct tgtttggtat gatctttttc aatctcctcg cggcgcgatt
     3001 tgtaatcggc ttcaagctca acaagagctg ccttacggcg ctcatcatca acttcgctta
     3061 caacataatt ggcgaaataa actacgcgct cgaggtcgcg gatgctcata ttgagcacta
     3121 acccaatact ggacggggtt cccctaagaa accatatatg ggtaaccgga acggccaaac
     3181 taatgtgccc cattcgttca cgccggacaa tactacgggt aacccgcaca ccacacttat
     3241 cgcaaacaac atctttataa cggatgcgct tgtactttcc gcagtaacat tcccagtcct
     3301 tggtcggacc gaaaatgcgt tcgcaaaaca gcccatcctt ttccggtttc tgtgtacggt
     3361 agttaattgt ctctggtttc gttacctcgc cgtgagacca ctccagtatt tgctggggac
     3421 tggccaaact taaacggacg gcactaaact cgctggcggt ttgatctaac ataagtggtt
     3481 ccatttaagc ctcctgctcc tgttcttccc gggcagaacc tgctgattcc tcggcctcag
     3541 cctgcagcgc atcttcttgc ccgccggcta agtctacgag cggcatatcg gccacggcat
     3601 catcgccttg aatcagcggc tcggttccga gctcatcaac ttcagtttca atatttttag
     3661 tcagtacgtc ttcagcatca acctcgacat ccgattcttc ccgaatcagg tccacactta
     3721 aacttaggga ctgcagttct tttactaaca cgttaaagct ttctggaatc cgcggtccgc
     3781 gaatttcttc ggatttaatg atcgcctcat aggctttcga ccggccgact acgtcatccg
     3841 atttaatcgt caaaatttct tggagggtat tggcggcacc ataggcttcg agtgcccaaa
     3901 cttccatttc cccgaaccgc tggccgcccc gctgcgcctt accgcccagt ggttgctggg
     3961 taaccatggc gtaaggaccg gttgaccggg catggatttt atcatcaacc atgtggttaa
     4021 gcttcaacat atatttataa ccgaccgtag tccgctcctg aaaggcctcg ccggttcgcc
     4081 cgtcgaaaag ctgggacttg ccatcttctg gtaagccagc tcgcttaagt tctgacttaa
     4141 ttttttcaat cgtaataccg ttaaagaccg gactggccac cgtataaccg agtgcttgcg
     4201 cggcccagcc catgtgagtt tcgaatacct gccccaagtt catacgggcc ccgactccga
     4261 gaggatttaa aatcatgtct actggagtac catcctcaag atgaggcatg tcttcaatcg
     4321 gcagtatccg cgcgatcacg cctttgttgc cgtggcgccc ggccatttta tcgcccaccg
     4381 aaaccttgcg catttgagcc accgaaacgt aatactgctc gattacgccc gctggcaact
     4441 catcgccgtt atcgcgggaa aagattttaa cgccaaccac cttaccccgc tcgccattgg
     4501 gcatacgcag gctggtgtcc ttgacgtcgc gggctttttc accgaagatt gctcgaagca
     4561 gtttttcttc gcttgatagc tcttgttcgc ccttaggggt aatcttccca actaaaatgt
     4621 caccggcagt aacttcggcg ccaatgcgca cgatgccggt ttcatctaaa tcttttaggc
     4681 tgtcttcgga aacattggga atatcacggg taacaacctc cggtccaagc ttggtatcgc
     4741 ggacgtcaat ttgatgggtc tcgatgtgaa ttgagctgta gcgatcatcc tgtaccaggc
     4801 ggcggctgag aatgatggca tcctcaaagt tgtatccgcc ccatgacata aaggctacca
     4861 agacattttg acccagggcc aactcgccgt tttcggttga cataccatcg gccaaaatct
     4921 cacctttttt gactttctgg ccgcttcgga caattgttcg ctggtgaatg ctggcaccat
     4981 cattactgcg gacgaagttc agcaaagggt aggttactgc atcgagattt ttgccctcgt
     5041 actgcacggt aatctcagcc gcactggctt gagacaccat cccgttggct tcagcaacaa
     5101 cgacctgtgc tgaatcgtga gccacaatac cttccatgcc ggtacccaca atcggagctt
     5161 ggggcttaac caaggcaacc gcctgcttct gcatgtttga gcccatcaat gcccgcttcg
     5221 ggtcgtcgtg ttcaataaat ggaatcaatg aggctgaaac cgatacgatc tgacgggttg
     5281 aaacatccat gtgctcaacg ttattggcat cctcttcttc agcttcaccg ccgcggcgaa
     5341 ccggaacccg cggttcaata aagtaattat caggaccaac cttggtgccg gcttcagtaa
     5401 taacggcgtc agcttcgtcg gccgcatcca taaagcggac ctcatcggtg actttagcct
     5461 taactggaac ggtgtcaagc ttcaacgccg ctagcttttt agcatgcgcc gccgtaatgg
     5521 tgtcgcccgg ctttacaatc actgtcccct tttcatcctt aatagttgta cgggcaatgt
     5581 agccgatagc atcattggga gcgattttat taatcacggt tcgatatggc gtttcaataa
     5641 acccatactc gttcacccgt gcataggtgg aaaggttaga aaccaagcca atgtttggtc
     5701 cttccggtgt ttcaatcgga cacagccggc catagtgact gcgatgaaca tcgcgaactt
     5761 caaacccagc tcgctcacga ctcaaaccgc cggcgcccat cgccgtaata cgacgcttat
     5821 gggcaatttc agctagcggg ttggtttggt ccataaactg actcatctgc gagctagcaa
     5881 agaactcctt tacgctagca actaccgggc gaacattaat aagctgcccc ggggtaacag
     5941 tttcacggtc gtacacgctc atccggtctt taacattacg ctccatgcgc aacaagccga
     6001 cccggaactt ttgctgaata agctcgccca ccatccgcaa gcggcgatta gcaagattat
     6061 caatatcttc cggttgagca ttggggtcgt tgttttgacg aatgatttca gcaataatcg
     6121 ccactagatc ctcacggcgg aaaattcgat tttcataggt gttaggaata tcaatagcca
     6181 gccgtttatt cagcttgtag cggccaacct tggaaaagtc atagcgcttc gggtcgaaga
     6241 acatcccttc aatcagcgac tttgagtttt caacagttgc cagatcgccg ggacgaatac
     6301 ggcggtagac ttccatcagc gcctcgctaa agtttgaggt agcatcttta tcaagcgtct
     6361 catcaataaa cttgatttcg ccggtatcaa ccttggcaaa caacgcttta atctctttgt
     6421 cctcagcata accgaatgaa cgcagcaggg tggtaaccgg cagcttgcgc ttacgatcaa
     6481 tctttacgct gattactccg ttaggtgcgg tatcgaactc caaccatgcg ccacggtcag
     6541 gaataacctt ggcgccgaat aaaagggccg atccggtgtt tt
//
LOCUS       DASVGB010000011         8864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1123497, whole genome shotgun sequence.
ACCESSION   DASVGB010000011 DASVGB010000000
VERSION     DASVGB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123497"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VF272_00255"
     CDS             <1..783
                     /locus_tag="VF272_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7259350.1"
                     /translation="DPYMKAQVDYAISKGVVVLAAAGNDGCNCMSYPANYPEVIAVGA
                     SDSANNTASFSSWGPNLDIIAPGTAGDVCSSAYTSTNATNAYTCSYAGTSLSTPIVAG
                     LAALMLQQHPGASPATIAALIAQAAAKLPSMGGAYFTLKQGYGLVQAGNAIARVTIPL
                     PFGQLLNKSSASLSSVSLATGADMDSTCMGIAGAPCTLTLSGPSGQILQIGQKILDEY
                     GGAEFAWNANSLGLNPGQWTVTATTTYAGQTKTNSIGLTVLP"
     gene            complement(811..1149)
                     /locus_tag="VF272_00260"
     CDS             complement(811..1149)
                     /locus_tag="VF272_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259351.1"
                     /translation="MNRPILIILSSVAGLTMVGAALLYTPSGSGGKNQIVSAAQLKEH
                     GGVNGRACWVAIDKKVYEISGFAQWQMGKHLPSNGKATCGNDLSKVILESPHGTSKLQ
                     LLKVVGTYQP"
     gene            complement(1149..2231)
                     /locus_tag="VF272_00265"
     CDS             complement(1149..2231)
                     /locus_tag="VF272_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259352.1"
                     /translation="MEHRMQQIVRHTWFLPVTILVVAVIASSVILATRLTGPDKQTAH
                     SQHFTYECELTNPKSMGCEQQHYARVTKTKNTAAAFAELKQSYNADPNTKAYCHQLTH
                     AIGRAAAEQSKDIEDAYAKGDNFCWSGYYHGVMELMVKKVGAKNIEAKITTICIKLAE
                     KRSYSFDHYNCVHGLGHGVMAINANELFDALKKCDRFQDQWEASSCHSGVFMENVMAG
                     LNPDTSTNYLKNDQPLYPCTAVGDRYKEQCYLMQTSQALKVVDYDYAEVFGLCATVGS
                     YSATCYQSLGRDASGNSNSDVAQTKATCSLGKTAEARSNCVEGAVKDFISYYHSDKQA
                     AELCQAFEPGLRDSCNAAKIEYYKSF"
     gene            complement(2241..3134)
                     /gene="gnd"
                     /locus_tag="VF272_00270"
     CDS             complement(2241..3134)
                     /gene="gnd"
                     /locus_tag="VF272_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX7259353.1"
                     /translation="MKIGFMGLGKMGQPMVGRLLEAGHEVIVRDLQPEAVKAAVEKGA
                     SEATDVAQMVQQLDPVIIWLMIPANLVDAQLDELLDQVGEGSIIIDGGNSDFYLTLER
                     SKKTAAKGVKLVDVGTSGGVLGGDQGYCLMVGGDKDAVAMLEPLFEALAQPGGYLNVG
                     PPGAGHFVKMVHNGIEYGAMQALAEGYHLMKAGQFKDLDLAAIGELWQHGSINESLLN
                     RLTVKALSENPEMEGIDGYVATSGEGEWAARTARDLGIPVPALEAALAVRAASQSGEV
                     NFGTKVLAAQRNQFGGHNLNT"
     gene            3303..3935
                     /locus_tag="VF272_00275"
     CDS             3303..3935
                     /locus_tag="VF272_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259354.1"
                     /translation="MSVSHRRTLPSPLYIIIAALFALCFVPASSAMADDDHACEVTQS
                     SAGGYSTRTYYAPPAQSGDHEDDDGDDDDRECATPITAPVKTPAALGTTSSTHIDNST
                     TNIDNSMHQTTINPPATSAPNTGSTAPPKKAKKCAWTRYHLMLMEVYSKSERRWVVIA
                     VPMRTKPPFSCVTKSMTSMLERYKARQRKIKAKGGKSTPARSAKGLSLAG"
     gene            complement(4039..4257)
                     /locus_tag="VF272_00280"
     CDS             complement(4039..4257)
                     /locus_tag="VF272_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259355.1"
                     /translation="MQQNLFLKSDSMKRVPAEDALVMALDRQEDELLLRFLTNDSLGS
                     VSQIRLEEDMASQLLQMLAAELHVPVGA"
     gene            complement(4433..4924)
                     /locus_tag="VF272_00285"
     CDS             complement(4433..4924)
                     /locus_tag="VF272_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259356.1"
                     /translation="MSATTQEDLHNRVERLETSLQKCRARIASLRGDLATAESHAVGL
                     EKKLVEARRAALQLRINELRPVVDESERLEAALVALDKHSLTKKKRSSRSRSRERGNH
                     AGGKRSQLALAIIQEKPGITIPEIARDMGIKSNYLYRAVPALADAGHIVKDETTKGWS
                     PKQ"
     gene            complement(5014..6141)
                     /locus_tag="VF272_00290"
     CDS             complement(5014..6141)
                     /locus_tag="VF272_00290"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7259357.1"
                     /translation="MITKRYGIYIVVAAAGIGLMVLAGTGHLQQFFFKPTPSLLKNGV
                     VSKNDNSKDIETIAQNLTVPWGIAFLPGGDLLVTERSGTLQRIGKDNQKHAIDGVQHV
                     GEGGLLGVALDPKFSANHRIYLYMTTKTGNALTNRIERYVYENRVLSQRQIILQNIPG
                     EANHDGGRLAFGPDGFLYVTTGDAGNSALAQNKSSLAGKILRLTADGLPAPGNPLNST
                     IYSYGHRNPQGLAWDKQGQLWATEHGRSGIQSGFDELNLIKPGANYGWPLIQGDETRP
                     GMEKPVAHSGPNETWAPASLAYANGSLFFGGLRGESLYEATITGDNTVALQVHFRGGY
                     GRLRTVAVNNQALYVTTSNTDGRGQAKPGDDKVIRIKLSVF"
     gene            6333..6674
                     /locus_tag="VF272_00295"
     CDS             6333..6674
                     /locus_tag="VF272_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259358.1"
                     /translation="MKKKNRKNIVIAAVTIVAVTIIALIVARASATPNASFTYTLTGN
                     QVSLVANPTCYMAPCSYTWYINDIKVGGQRSFVTTLAPGCSSVRLLKQERTYRNYTNP
                     RAETTQTICVN"
     gene            complement(6722..7097)
                     /gene="ssrA"
                     /locus_tag="VF272_00300"
     tmRNA           complement(6722..7097)
                     /gene="ssrA"
                     /locus_tag="VF272_00300"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            7276..7497
                     /locus_tag="VF272_00305"
     CDS             7276..7497
                     /locus_tag="VF272_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259359.1"
                     /translation="MQDQPQGGDAREDQDIQLEFYEQLGHDDQARLSEEMGYIENETD
                     VDTFGETLGDSAAEGAADPFMAEEKEDLS"
     gene            complement(7490..8758)
                     /locus_tag="VF272_00310"
     CDS             complement(7490..8758)
                     /locus_tag="VF272_00310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7259360.1"
                     /translation="MLYLRLFKYADFAKLWSAQILSGISQNLLNFSLIILVYELTLGS
                     QFANLSVSLLVLTFGLPALLFAPIAGAYVDHWDRKKVLVVSNLLRAGLVLLYVFFDHN
                     LSLILIISFIVSTILQFFVPAESASIPNVVPKNLLLAANSLFIFSLYASFIIGYSGSG
                     PAIHYLGPHGPYWVVAGMLLLAALLTSTLDLRSVKRRSGPLPRLHPVKHLRNNWRIIT
                     SQRDRLFSALQLTVTQAMVSVLITLAPALSLAVLHIPLKQASHILIIPVGVGLVLGVV
                     LVTTISKRLTKMRLIQGGLITAGITLTLVGLSGQLYRTHDGQTLLPLAVIPVILAVLM
                     LLLGMLNALISAAAQTMLQESTNDENRGKVFASLNMMVNLAATTPILLTGLLADLLSV
                     TKVITMLGVIATVYALYITWRYRRSVVQPS"
BASE COUNT         2179 a         2466 c         2206 g         2013 t
ORIGIN      
        1 gatccctata tgaaggcaca ggttgattat gctatctcaa aaggagttgt cgttttagcg
       61 gctgccggca atgacggctg caattgcatg tcgtacccgg ctaactatcc ggaggttatc
      121 gccgttggtg ccagcgatag cgccaacaat accgccagct tttccagctg gggccctaat
      181 ctcgacatca tcgctccggg aacggcaggc gatgtctgct cgagcgccta taccagcacc
      241 aatgccacca acgcctatac ctgctcctat gccggtactt ccttatcaac cccgattgta
      301 gccggcctag cagccctgat gctccagcag catcccggtg ccagcccagc cacgattgca
      361 gcgctgattg cccaggctgc cgcaaagctg ccctcaatgg gaggcgctta ctttaccttg
      421 aagcaaggct acggcctggt gcaggccggg aatgccatag cccgggtaac gattccgctt
      481 ccattcggcc agctcctcaa taaatcctcg gccagccttt ccagcgtctc gcttgcaacc
      541 ggagccgaca tggatagcac ctgcatgggc attgccggag caccctgcac cctcacccta
      601 tccggaccaa gcggccaaat cttgcagatt ggccagaaaa ttctcgatga gtacggaggg
      661 gccgagttcg cttggaatgc aaatagccta ggcttaaatc ccggccaatg gacggttacc
      721 gcaaccacca cctatgccgg tcaaaccaaa actaactcaa ttgggctgac ggtgctgcct
      781 tgatagctta tttcctcctt atcgagctta ttacggttga tacgtcccga ccaccttcag
      841 cagctgtaat ttcgatgttc catgcggcga ctccagaatt acctttgaaa gatcattgcc
      901 gcaggttgcc ttgccgtttg aaggcaagtg cttccccatc tgccactggg caaacccgct
      961 aatctcgtac acttttttgt caatcgctac ccagcacgcc cgaccattca ctccgccatg
     1021 ctccttaagc tgagctgcgc taactatctg atttttaccg cctgaaccac tcggcgtata
     1081 cagcagtgcc gcgccaacca tcgtcagacc ggccacggag ctgaggatga tgaggattgg
     1141 ccggttcatc agaagctctt gtagtattcg attttagcgg cattgcacga gtctcgtaag
     1201 cccggctcga aggcttggca cagctcagcg gcctgcttat cgctgtggta gtaggaaata
     1261 aagtccttca ccgccccctc tacgcagttg gagcgagctt cagcggtttt accgagagaa
     1321 caggtggcct tggtttgcgc gacatcgcta ttactattgc cggaggcgtc gcgccccaaa
     1381 ctctgataac aggtggccga gtaactgccc acggtagcac acaaaccaaa gacttcggca
     1441 taatcatagt ccactacttt caatgcctga gaggtctgca taagatagca ctgctccttg
     1501 tagcgatcgc caacggccgt acagggatac aagggctgat cattctttaa atagtttgtt
     1561 gaggtatcag ggttaaggcc agccatgaca ttttccataa aaacgccgct atgacaagat
     1621 gatgcttccc actgatcctg aaaccggtcg cacttcttca gcgcatcaaa tagctcattg
     1681 gcgttaatgg ccatgacgcc atggccaagc ccgtggacac agttatagtg gtcgaacgaa
     1741 tacgagcgct tttcggctag tttgatgcaa attgttgtaa tttttgcttc aatgttcttc
     1801 gcgccaacct tcttgaccat gagctccatt acgccgtgat agtagcctga ccaacagaag
     1861 ttatcacctt tagcgtaggc atcttcgatg tctttcgact gctcagcggc agcccggcca
     1921 atggcatgag taagctgatg acagtaggct ttggtgttcg ggtcggcgtt gtaagactgc
     1981 ttgagttcag cgaaggctgc ggcagtattt ttcgtctttg taacccgtgc ataatgttgt
     2041 tgttcacagc ccatactctt cggattggtg agctcgcatt cataggtaaa gtgctgagaa
     2101 tgtgcggttt gcttatctgg tccggttaat cgcgttgcca gaattacaga tgaagcaatg
     2161 acagcgacca ccaggatcgt aaccgggaga aaccaggtgt gtctgactat ttgctgcatg
     2221 cggtgctcca tagtaacaga ttacgtgttt aaattatgtc cgccgaactg attgcgttga
     2281 gcggccagca ctttagtgcc aaagttgacc tccccagatt gggaagctgc tcgcacggca
     2341 agagccgctt cgagcgctgg aaccggaatt cccagatcac gggcggtccg ggcagcccat
     2401 tcgccttctc cagatgtagc gacataacca tcgatacctt ccatctcggg gttttcagat
     2461 aaagccttaa cagtgaggcg gttcagtaat gattcattga tgctgccatg ctgccacagc
     2521 tcgccgatgg cggccagatc aagatcctta aactgaccgg ctttcatcag atggtagcct
     2581 tcggctaagg cctgcattgc accgtactcg atgccgttat gcaccatctt gacgaagtgc
     2641 cctgctccag gcggaccaac gttcaaatag cctcctggct gcgctagagc ctcgaagagg
     2701 ggctccagca ttgctaccgc gtctttatcg ccccctacca tcaagcaata cccctgatcg
     2761 ccccctaaaa cgccgccgct ggtaccaaca tccaccagct ttaccccttt agcagcggtt
     2821 ttcttgctgc gctccagagt aaggtagaaa tccgagttac cgccatcaat gataatgctc
     2881 ccttcgccaa cctgatctaa cagctcatcc agctgagcgt ccaccaggtt agccgggatc
     2941 atcaaccaga taataaccgg atcaagctgt tgtaccatct gggccacatc tgtagcttct
     3001 gatgcgcctt tttccaccgc tgccttcacc gcttcaggtt gaaggtcgcg cacaatcacc
     3061 tcatgtccag cctctaacag ccgtccgacc atgggctgtc ccatcttacc taggcccata
     3121 aatccgattt tcattggttg ctccttccag caagaatagt cttcctagta tagcctatgg
     3181 ccagcgccct tatccaccgt aagaatcaat agataattct tgacaaaatt agataaatgt
     3241 agtaatatag ttgccacgtc ggtattctcc gacaatcccg atccctcgaa aggggttcct
     3301 gcatgtccgt ttcacaccgc cgtaccttgc caagcccgct gtacatcatc atcgccgcgc
     3361 tcttcgcgct gtgcttcgtc cccgcctcgt ctgcgatggc cgacgacgac cacgcctgcg
     3421 aggtcaccca gtcgagtgcg ggtggctaca gcactcgcac ctactacgcc ccgcccgccc
     3481 agtcgggcga ccacgaggac gatgacggag acgatgacga tcgtgagtgt gcgacgccga
     3541 tcaccgctcc ggtgaagacg ccggcggcgc tcggcacgac ctcgtcgacg cacatcgaca
     3601 actcgacgac aaacatcgac aactcgatgc accagacgac gatcaacccg cccgcgacgt
     3661 ccgcgccgaa cacgggctcg acggcaccgc cgaagaaggc gaagaagtgc gcgtggaccc
     3721 ggtatcacct catgctcatg gaggtctact ctaagagcga gcggcgctgg gtcgtgatcg
     3781 cggtgccgat gcgcaccaag cccccgttca gctgtgtcac caagagcatg acctccatgc
     3841 tggagcgcta caaggcgcgt cagcgcaaga tcaaggcgaa gggcggcaag agcacgccgg
     3901 cgcgctccgc gaagggcctc agcctcgcgg ggtagcttct ctagcaccat ctcggtgcat
     3961 gcgggttccc ggtcgacgac atcatgtcgg ccggggcccg ccccactagc aattatttct
     4021 caatagcact tcccattatt acgccccgac aggcacgtgc aactcagcag ccagcatttg
     4081 cagcagttgg ctagccatat cttcctctag ccgaatttga ctgaccgatc ccagtgaatc
     4141 gttggtcaag aacctaagca gcaactcatc ttcctggcgg tcaagcgcca taaccaaggc
     4201 gtcttcggcc ggtacccgtt tcatactatc tgatttcagg aataaatttt gttgcataac
     4261 ccttctcccc ttctataaaa ccaggataaa ggtgtgaagc atatacgtca ttcagcagag
     4321 ttacaacata aactttttgc ccgaattttt agaagtttta ccattgaata agtgctaaaa
     4381 agttgaagta ctgtgccggg ggcggactgc tgcccccggc acatcgcgcg tactactgct
     4441 tgggcgacca gcccttggtg gtctcgtcct tgacgatgtg accggcgtcg gccagcgcag
     4501 ggaccgcgcg gtagaggtag ttcgacttga tgcccatgtc ccgagcgatc tccgggatgg
     4561 taattcccgg tttctcctgg atgatcgcca aggccagttg gctccgcttg ccacctgcgt
     4621 ggttgccacg ttcccgagaa cgcgagcggc ttgagcgctt cttcttggtg agggaatgct
     4681 tgtccaacgc caccaaggca gcctcgagcc tctcgctctc atccaccacc ggtcgaagct
     4741 cattgatccg cagctgcagc gcggcccgcc gagcctcgac cagcttcttc tcgagcccca
     4801 cagcatggga ctcagcggtg gcaagatccc cgcgcagaga ggcgatacga gcacgacact
     4861 tctggaggct cgtctcaagc cgctcgacgc gattatgcaa atcttcctgc gtggttgcac
     4921 tcattggccc tcctcgggtc ctagtggttg tcaggagaat tcaagcttta ctgttatatc
     4981 ataatgagaa tataattaca agaagttgat cctttaaaaa accgaaagct taatccgaat
     5041 aaccttatcg tctcccggct tggcttggcc gcgtccgtcg gtatttgagg ttgttacgta
     5101 aagcgcctga ttgttcaccg ctaccgtacg caagcgcccg taacctccac ggaaatgaac
     5161 ctgcagcgca accgtgttat ccccggtaat cgtggcctca tataatgact ccccgcgtaa
     5221 tccgccgaag aacagcgaac cattggcgta tgctaagctg gctggtgccc aggtttcatt
     5281 cggcccggaa tgagctaccg gcttttccat tcccggacga gtttcatcgc cttgaatcag
     5341 tggccaccca taatttgcac ccggcttaat caaattcagt tcgtcgaacc cagactgaat
     5401 tcccgagcgg ccatgttcgg tagcccagag ttgcccctgt ttgtcccagg ctagaccctg
     5461 agggttgcgg tgaccatatg aataaatcgt tgaattgagg ggattccccg gggcgggtag
     5521 gccgtctgcc gtaagtcgca aaatcttgcc cgctaatgag cttttgttct gcgctagagc
     5581 tgaatttcca gcatcgcccg ttgtcacata caaaaaacca tcagggccga atgccaaccg
     5641 gccgccatca tggttcgctt caccgggaat attttgcaaa ataatctgtc gctgcgatag
     5701 cacccggttc tcgtaaacgt agcgttcaat ccgattggtt aaggcgttgc cggtcttagt
     5761 cgtcatatat aagtaaatac ggtggttcgc actaaacttc gggtcgagcg ctacccccaa
     5821 aaggccgccc tcaccgacat gctgcacgcc atcaattgca tgtttttgat tatcttttcc
     5881 gatgcgttga agcgtgccgg agcgttccgt taccaataaa tcaccgcccg gcaaaaaggc
     5941 gatcccccag ggtaccgtga ggttttgggc aatggtttca atatccttac tgttgtcatt
     6001 tttactcaca accccatttt taagcagcga aggagtcggc ttaaaaaaga attgctgaag
     6061 atggccggta ccggcgagca ccatgaggcc aattccggcc gctgccacca ctatataaat
     6121 accgtaccgc ttggttatca tctttacagt atagtacgaa acgctagcaa atagcggcga
     6181 ataccttccc ttaataaact cccacgctta gggatatatc actgcaagaa aacaaacaaa
     6241 aagcgaacga tttaaacata agcgtttaca tcctgtaaac tctgggtata attggggtta
     6301 ttagtaaagc caatcaaatg ggagcgttta ttatgaagaa gaagaacaga aaaaatattg
     6361 taatcgctgc ggttactatt gtcgcagtca ccatcatagc tctgattgtc gcgcgtgcca
     6421 gtgcgacgcc taatgcgtcg ttcacctaca cgctaaccgg caaccaggta tcgctggtgg
     6481 ctaatccaac ctgctacatg gctccatgca gctatacgtg gtacataaat gacattaagg
     6541 tgggaggaca gcgctcattc gtgactaccc tcgcccccgg ttgtagtagc gtgcggctgc
     6601 ttaagcaaga acgcacctac cgcaactata ccaatccccg agcggagacg acccaaacaa
     6661 tctgcgtcaa ctaaagctga cgttccgacc aaaaaagccc cagcaaatgc tgaggctttt
     6721 ttggtggagc tgcggggaat tgcaccccgg tccggaagtt tactagctac tcatctacag
     6781 gcatagaccg ctttaagtct tagcgcgcgg ccttgaagcg gaccaaatgg tcgcgcgagc
     6841 ggccaactgt cttagcgtta agcgtctggc gtcgcttaac gagcacccag atgatataac
     6901 accggccaac ccctatctgg agtcaggtgc cgatggctta cgacaattaa gcgtaagctg
     6961 gtgaaagtgt gggaacgcta aaagcgttct ttaccttcga aacaaggtta ttgagacttg
     7021 tttgttggtg gatttacgca tgcctgcgac ctgccgagta gactgtaaag gatctcccgt
     7081 cgaagctaga cagccccatg gatccctatt ctatctgaaa aagaccgaga gataaagcct
     7141 agtggtaccg tcaatgtcac gaagaataga tgagaaaatg ctgctatttt aaacgttctg
     7201 caatagagtc aacctggtag aggcctgtac aatacgtagt ctaaattaat ctaaacgttc
     7261 ttagaggagg catccatgca agaccaaccg caaggtggcg acgccagaga agatcaagat
     7321 attcagctcg aattttacga gcagttgggt cacgatgacc aagcccgcct gagtgaggag
     7381 atgggctata tcgaaaacga aactgatgta gacaccttcg gtgaaaccct gggagatagt
     7441 gctgcagaag gagctgccga tccattcatg gcagaagaga aagaagacct tagctaggct
     7501 gcacaactga ccggcgataa cgccaagtga tgtagagcgc atagaccgtg gcaatcacgc
     7561 ccaacatggt aataactttg gtaacactta gaaggtcggc caataagccg gtaagcagaa
     7621 tcggcgttgt ggccgccaaa ttaaccatca tgttgagcga tgcaaaaacc ttgccgcggt
     7681 tttcgtcgtt cgtactctct tgcagcatgg tttgagcggc tgcactaata agcgcattca
     7741 gcatgccgag cagcagcatt agtaccgcca gaatcaccgg gataacagcc agtggaagca
     7801 gggtctggcc gtcgtgtgtc cggtaaagtt gtccgcttaa cccaaccaaa gttagcgtga
     7861 tgccagccgt gattaagccg ccttgaatca gcctcatctt tgttagccgt ttgctaatcg
     7921 ttgtaaccaa caccacacct agcactaatc ctacccccac cggaataatc aaaatatggc
     7981 tggcttgctt cagtggaatg tggagtaccg ctaaactgag tgccggcgcg agcgtaataa
     8041 gcaccgatac catcgcctga gtcaccgtaa gctgcaaggc ggaaaacaag cggtcccgct
     8101 gcgaggttat aatacgccag ttgttgcgta gatgtttaac tggatgcagc ctgggaagcg
     8161 gtccgctacg gcgttttaca ctccttaaat cgagcgttga ggtaagcagc gcggccaata
     8221 gcagcatgcc ggcaaccacc cagtacggcc catgcggacc aaggtaatgg atggccgggc
     8281 cagagccgga gtatccaata ataaaagatg catacaggct gaaaataaac agcgaattgg
     8341 ctgctaataa gagattcttc ggaaccacat tgggaatact ggcactttca gccggaacga
     8401 aaaattgcag aatagtcgag acaataaagc tgatgatcag gatcagcgac aaattatgat
     8461 cgaaaaatac atacagcagc acaaggcctg cccgcagcaa attagataca acgagaacct
     8521 ttttgcgatc ccaatggtct acatacgcgc cggcgatcgg ggcaaacaac aacgccggca
     8581 acccaaaggt gagcaccagc agagaaaccg ataggttggc gaactgagag ccaagggtga
     8641 gctcgtaaac cagaataatt aaggagaaat tgaggaggtt ttgactaatg cctgagagga
     8701 tctgggcaga ccacagcttg gcgaagtcag cgtatttgaa taaacgcagg taaagcacat
     8761 cggtatgata caggatatgg ctaaaaacgt ttagcgggga ttatgtttta ccgcagcttc
     8821 ccacgcaagc aaaaataatc cgcgcattgc cttagagagc tcac
//
LOCUS       DASVGB010000012         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_979669, whole genome shotgun sequence.
ACCESSION   DASVGB010000012 DASVGB010000000
VERSION     DASVGB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979669"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VF272_00315"
     CDS             <1..886
                     /locus_tag="VF272_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259361.1"
                     /translation="VSRRRLRYEWWYGIHLMVYAAILLGFGHQLKIGGDFIGHDWFIG
                     YWYFLYILAFSLLGWFRFGRPTWRALRHRFTIDRVVQEADNIHSIYVRGRNIDTLKPK
                     PGQFFVWRFLDGERWWQSHPFSMSQLVRPNELRLTFKAVGDFTRRLHLVKPGTPALLD
                     GPRGNFTLDLAQNNKLLFVAGGIGITPLRTMIEALPKKVDAQLIYICRNQKECALEDE
                     LNQLSTGKGVKVHYIYSEKAGRLTPERLSELVPDIVKREIFLCGPEPMMKAMTTMMVG
                     LGVKKQRIYSERFAFLGI"
     gene            947..1732
                     /locus_tag="VF272_00320"
     CDS             947..1732
                     /locus_tag="VF272_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7259362.1"
                     /translation="MSRSHHHSRPVHLHQRIYGLLFIPVILLVAAYVVAIPASVGGGD
                     VSAVELGAALCLSFVRLLAAFVLALLVAIPFSLLIHRSPLVERSVLPLLDTSYSVPVL
                     AFFPVLILVFNQYHFLEGAAVSLIVITMVWALVINSVAGLRSIPSDIHDTAKVLGIKG
                     LKKIRHVVLPAIIPSIVSGSLLAWAAGWNMLLVAEVLHNYTPGRSSEGDLFGIGSVIV
                     HAAAAGQNSLFIGAMITMLAAILLMNFFIWQRLQDYAERYRFE"
     gene            1725..2447
                     /locus_tag="VF272_00325"
     CDS             1725..2447
                     /locus_tag="VF272_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7259363.1"
                     /translation="MSKIIELEAVQKIHPGKSRPSLHPLSFFALESEFIVIIGPSGCG
                     KTTLCRLISGLEEPTAGTLTRPDEVAMVFQSGALFPWLTVAQNLAMVLEVRGIAPGQV
                     KRKVRMHLEMVGLHSSARAYPRELSAGQRQRVGLARAMAIDSPVMVLDEPFAALDIKT
                     TALLHDDLLRLGQETKKTIIMVSHSIEEAVTLADRILLMNAGRIVRTYHLADLAYPRR
                     EAAENFIHQVHAIRRDFLALGE"
     gene            2496..>2961
                     /locus_tag="VF272_00330"
     CDS             2496..>2961
                     /locus_tag="VF272_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7259364.1"
                     /translation="MPNPTRAKVPTSVHPKTAPWWQTSVIYQLYVRSFFDTNGDGVGD
                     LPGVIAKLGYLEELGVNAVWLSPITESANVDWGYDVSDYYAIDPSLGTMEDFDRLMSE
                     AEKRNIKIIMDLVPNHTSSQHPWFLNALTGRHAKYRDYYIWADPKPDGRPPNN"
BASE COUNT          661 a          675 c          815 g          810 t
ORIGIN      
        1 ggtctcccgc cgccgcctgc gctatgagtg gtggtacgga attcatttaa tggtctatgc
       61 tgccattttg ctgggattcg ggcaccagct aaaaattggc ggtgacttta ttgggcacga
      121 ttggtttatt ggttattggt actttttata cattttggcg tttagccttt taggctggtt
      181 ccgtttcggc cggccgactt ggagggcgct tcgtcatcgg tttacgattg accgggtggt
      241 tcaggaggcc gacaatattc attcaattta cgtgcgcggc cgcaacattg atacgctaaa
      301 gcctaagccg ggacagtttt tcgtatggcg gtttttggac ggcgagcgtt ggtggcagtc
      361 gcatcccttt tcaatgtccc agctggtgcg gcccaacgag ttgcgtttaa catttaaagc
      421 cgtaggcgat tttaccagac ggctccatct agtgaagccc ggtacgccgg cattgcttga
      481 cggtccgcgc ggcaatttta ccttggacct agcgcagaat aataagctgc tgttcgtagc
      541 gggcggaatt ggtattacgc cgctgcggac aatgattgag gccttgccaa aaaaagtcga
      601 tgctcaactg atttatatct gccgcaacca aaaagaatgt gctttggaag atgaactaaa
      661 ccagctgtcc acgggaaaag gggtgaaagt acattatatt tacagcgaga aagccggacg
      721 tctgaccccg gaacggttat ccgagctggt gccggatatt gtgaaacgcg aaatcttttt
      781 atgtggtccg gaaccgatga tgaaggctat gactaccatg atggtggggc tgggggtgaa
      841 aaagcaacgc atttattccg agcgctttgc tttcctgggg atctaaggcg ttaatctagt
      901 atttgcctgc ggttactgac ataatgacgc taggagcata agcgttatga gccggagcca
      961 tcatcattca cgtcccgtgc acttgcatca gcgtatttac gggcttttat tcataccggt
     1021 tatcttgctg gttgcggcct acgtggttgc cattccggcg tcggttggcg gcggggatgt
     1081 ttcggcggtt gagctggggg cggcactatg cctgagtttc gtcagactgt tagccgcctt
     1141 tgttctggcg ttgctggtag ctatcccttt ctccctcctg attcatcgca gccctttggt
     1201 ggaacgcagc gttcttccac tgcttgatac ctcctattcg gtaccggttt tggctttttt
     1261 cccggtactg attcttgttt tcaatcagta tcattttctg gaaggagcgg ccgtatcgtt
     1321 aatcgttata acaatggtgt gggcgttggt tattaacagc gtggctgggc ttagatcaat
     1381 tccgtcagac attcacgata cggccaaagt tcttgggata aaaggcttaa aaaaaattcg
     1441 tcatgttgta ttgccggcaa ttattccatc gatagtaagc ggttcgttat tggcctgggc
     1501 agcaggatgg aatatgctgc tggtggccga agttctacat aactatacgc ctggaaggtc
     1561 gagcgagggt gatctttttg ggattggcag tgtaattgtt cacgcagcgg cggcgggaca
     1621 gaacagctta tttattggag cgatgatcac catgctggca gctatactgc tcatgaactt
     1681 ttttatctgg cagcgcctgc aggactacgc cgagaggtat cgttttgagt aaaatcattg
     1741 agcttgaagc ggtacaaaag atccatcccg ggaaaagccg gccgtcattg catccattgt
     1801 cgttttttgc ccttgaaagt gaattcattg tcattattgg tccgtcggga tgcggaaaaa
     1861 ctacgttgtg tcgtctaatt agcgggttgg aagagccaac cgctggaaca ctgacccggc
     1921 cggacgaggt ggcgatggta tttcagtccg gcgccttatt tccctggctg acagttgcac
     1981 aaaatttggc catggttttg gaggtgcgcg gtattgctcc ggggcaggtg aagcgcaagg
     2041 ttcggatgca tttggaaatg gtgggactac actcaagcgc cagagcctat ccgcgtgaac
     2101 tttcggccgg ccagcgtcag cgcgtcggtc ttgcgcgggc aatggcaatc gattccccgg
     2161 taatggtgct ggatgagcca ttcgccgctc tagatataaa aactaccgcc ttgcttcacg
     2221 acgatctttt gcggttaggg caagaaacca agaaaaccat tattatggtt tctcattcca
     2281 ttgaggaagc ggtaactctg gcggatagaa ttttattgat gaacgcgggc cggattgtgc
     2341 ggacgtacca tctggcggat ttggcatatc cgaggcgcga ggcggctgaa aattttattc
     2401 accaggtgca cgcaattcgg cgcgattttt tggcccttgg agagtaaagt caagtctttt
     2461 aattttaccc ttacgacgta taatgcaagc tatctatgcc taacccaact cgagcgaaag
     2521 tgcccacatc ggtgcatcca aaaacggccc cctggtggca aacatcggtt atctaccagc
     2581 tctatgtccg atcgtttttt gatacgaatg gagatggggt gggtgatctt cccggcgtaa
     2641 tcgccaagct tggatattta gaagaactgg gcgttaatgc ggtttggttg tcgcccatca
     2701 ccgaaagcgc caatgtcgac tggggatatg acgtgtccga ctattacgct attgatccaa
     2761 gccttgggac catggaagac tttgaccggc taatgagtga ggcggagaag cgcaacatca
     2821 aaataattat ggacttggtt cccaaccata ccagcagtca gcacccttgg tttctaaacg
     2881 ctttaaccgg ccgtcatgca aaataccgtg attattatat atgggctgat cccaaaccgg
     2941 atggacggcc tccgaacaac t
//
LOCUS       DASVGB010000013         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_553545, whole genome shotgun sequence.
ACCESSION   DASVGB010000013 DASVGB010000000
VERSION     DASVGB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553545"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..206)
                     /locus_tag="VF272_00335"
     tRNA            complement(117..206)
                     /locus_tag="VF272_00335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Ser,seq:tga)
     gene            320..913
                     /locus_tag="VF272_00340"
     CDS             320..913
                     /locus_tag="VF272_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259365.1"
                     /translation="MDPQPSDQQPDSHASSPDASSLGQPPDSDSQAQLQPSDENSVDP
                     HEVAAFGWEASEFVLHEKPVVWYVGVIAAAAVFCAGLAWFKQWIAIALVAVMTLAVIS
                     FSRKEPRVLQYMVDDDGITIDGKLHDYYLFKSYSLHPQVGWREIDFEPAKRFSQRLTV
                     LCEDEAFDHIEAVLSVHLPRVDREPDFIEKLTRYLKF"
     gene            964..1040
                     /locus_tag="VF272_00345"
     tRNA            964..1040
                     /locus_tag="VF272_00345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:998..1000,aa:Arg,seq:gcg)
     gene            1120..1389
                     /locus_tag="VF272_00350"
     CDS             1120..1389
                     /locus_tag="VF272_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX7259366.1"
                     /translation="MASTQGIKLDDETRNRLKALGEKRDRSPHWLMRTAIESYLEREE
                     KYEREKAEDMQRWEQYQLAGKALDQTAAEAWLNSLSQGKSASWHQ"
     gene            1377..1676
                     /locus_tag="VF272_00355"
     CDS             1377..1676
                     /locus_tag="VF272_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7259367.1"
                     /translation="MASVKWLPEALDDVERLYYFLRDKSPQAAMRAANSILQGAILLK
                     ATPRLGRPMDDATGRRELFISFGAGAYVLRYMLEKKDVVIVICVWHSREDKPASD"
     gene            1887..>2491
                     /locus_tag="VF272_00360"
     CDS             1887..>2491
                     /locus_tag="VF272_00360"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX7259368.1"
                     /translation="MRQSIQFKREHIPYLIAFAIILAMYLPRTFAASAIEGTVFKDLN
                     RNSVQDPGELGVAGLQIGLIDKASGSGIAYATTDANGHYTIAEKSFSAGGGPIPDGNY
                     LAYISAGSEGWWAYRNEWFPTDNTVSVWPLSVFSLAGSASVNFPIRPIVQSSDRNQPI
                     SSYVGSSGLKTHSFNDVYDAKDVHDSIMRGGYVGEEAKTVT"
BASE COUNT          628 a          564 c          636 g          663 t
ORIGIN      
        1 gttacagtat gaaagcatac aggagaccca gggctgaatc aatcgctacc gccactagtc
       61 cttcgacgtt aagcagtagc ggtttatgcc cggccagggc gtacgtgcaa tatagctggc
      121 ggagggggtg ggattcgaac ccacgaggat ctttcaaccc tgccggtttt caagaccggt
      181 tccttcaacc actcggacac ccctccaggg gccacacttc agcataccag tcccctgcct
      241 tcagaatcaa ttgattgcct gagtagttaa ttgttttcaa atcgccgctc atgcataata
      301 taaagcataa ggattttata tggacccgca gccctcagat caacagccag attcgcacgc
      361 tagctcgccg gacgcttcca gtctcggtca gccaccggat tctgattcgc aagctcagct
      421 tcagccttcc gatgagaatt ctgttgatcc gcatgaggta gcagcatttg gctgggaagc
      481 ttcggagttt gtactgcatg aaaagccggt cgtctggtat gtaggagtaa ttgcagcggc
      541 ggcggtgttc tgtgccggtc ttgcttggtt caagcagtgg atagccattg ctttggtagc
      601 ggttatgact cttgcggtta tctcattttc tcgcaaggag ccgcgagtat tgcagtatat
      661 ggttgatgac gacggaatta ccattgacgg caaactgcat gactattacc tctttaagtc
      721 atatagcttg catcctcagg taggctggcg ggaaattgat tttgaacccg ctaagcgctt
      781 ttcccagcgg cttaccgtgc tttgcgaaga tgaggcgttc gaccatattg aagcggtgct
      841 gtcggtgcat ttaccacggg ttgatcgcga acctgatttc attgaaaaac ttacccgcta
      901 cttgaagttt taagcttccg tgcatcccta aattatgata atctcttgga gtcttggcgt
      961 cgtgcgcccg tagctcagct ggatagagcg tcagtttgcg gtactggagg tcgcaggttc
     1021 gaatcctgcc gggcgcacca ttggacttat tattctttta cgccggttgc gcaaagttgc
     1081 aataagtact actatggctg acataaggaa gaaaagcacg tggcttccac acagggtatt
     1141 aaactagatg atgaaactcg aaatcgtctc aaggcattgg gtgaaaagcg tgatcgttca
     1201 ccgcactggc tcatgcggac cgctattgaa agctatttgg agcgggagga aaagtacgag
     1261 cgcgaaaaag cagaagatat gcagcgctgg gagcaatatc agctagccgg taaggcactt
     1321 gaccagacgg ctgctgaagc atggttgaat agcctttcac agggcaaatc cgcttcatgg
     1381 catcagtaaa atggcttccc gaagctctag atgatgttga gcggctttac tatttcttac
     1441 gtgacaaaag cccccaagca gcaatgcgag ctgcaaacag tattttacaa ggggctattt
     1501 tgctcaaagc cactccgcgc ctcgggcgac cgatggatga tgcaactggt aggagggagc
     1561 tgtttatatc gttcggcgcg ggagcctacg tactgagata catgctagaa aagaaggatg
     1621 ttgttattgt gatatgtgtc tggcatagcc gggaagataa gcctgcttcg gactaactat
     1681 ttatctgaaa tagttagtcg catgacaata aaatggttct aacctcggcc agcgtgctgc
     1741 accattggac ttatgcctat cttactataa tgttgagtct cggttaagat ttacaatcct
     1801 tcagggcaaa agcgtttgca tttattaata agcataagta ttatacaggc agtactcagc
     1861 actaattttt gatcggtatg accttaatgc gtcaatcaat acaatttaaa agagaacata
     1921 tcccctacct cattgccttt gcgataattt tggcaatgta tcttccccgg acgtttgctg
     1981 ccagtgccat tgagggaacg gtttttaaag atctaaaccg aaatagcgtt caagatcctg
     2041 gtgagcttgg tgttgccggg ctgcaaattg gccttataga taaagcctcg ggtagtggga
     2101 tagcgtacgc tactaccgat gccaatgggc actatacaat tgccgaaaaa tcattcagtg
     2161 cgggtggcgg cccaattcct gatggaaatt atttggcata tattagtgct ggtagtgaag
     2221 gctggtgggc ttatcggaat gagtggtttc ctacagacaa cactgtatcg gtctggccat
     2281 tgagcgtttt tagcttagct ggctctgcaa gcgttaattt cccgattcga ccaattgttc
     2341 agtcatccga cagaaatcaa ccgatttcgt cgtatgtagg gtctagcggg ttaaaaactc
     2401 acagctttaa cgacgtctat gatgccaagg acgtccacga ttccatcatg cgtggaggct
     2461 atgttggcga ggaagccaaa acagtgacta t
//
LOCUS       DASVGB010000014         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_693252, whole genome shotgun sequence.
ACCESSION   DASVGB010000014 DASVGB010000000
VERSION     DASVGB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693252"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..345)
                     /locus_tag="VF272_00365"
     CDS             complement(100..345)
                     /locus_tag="VF272_00365"
                     /inference="COORDINATES: protein motif:HMM:NF039192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HEX7259369.1"
                     /translation="MDWSNIMKQYKGKWVALADDEKTVVASAKRAIDASKLAEKPLRR
                     HYAGILGQKGFFEDYSVKFEYPSQIIIMPIPLQLTDN"
     gene            879..1427
                     /locus_tag="VF272_00370"
     CDS             879..1427
                     /locus_tag="VF272_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259370.1"
                     /translation="MTSTQTQGAKQMFDNAVAMQSTQPEAAASIFGSIGENVEGPLGQ
                     AAREQLISTLCALGRSDQALRASAKLMQEIRDNSKQSYFWARVAVRWAELSIANGRES
                     DASQVLEQAERIFELNGDYHYVGKVAELKAAISTAKGDYDKADIQAKRGQRLTVTHAP
                     AVTPGRTSSAPELAGIVSSSNN"
     gene            complement(1506..1886)
                     /locus_tag="VF272_00375"
     CDS             complement(1506..1886)
                     /locus_tag="VF272_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7259371.1"
                     /translation="MFKDTPAFSGFSVNDLAAAKKFYGDTLGIETEETPQGLSLKITG
                     GKGLFIYPKDNHEPATYTVLNFSVDDIDKAVEELAGKAIKFEHYNGMTDDKGIARGLA
                     ANMGPDIAWFKDPAGNVLAVLQEG"
     gene            complement(1903..2082)
                     /locus_tag="VF272_00380"
     CDS             complement(1903..2082)
                     /locus_tag="VF272_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259372.1"
                     /translation="MKTHSSYERLAESVEELGITKSNMFGMPVLKLGRKPVAGLASDG
                     INQICGIRTSKLVIK"
     gene            2194..2679
                     /locus_tag="VF272_00385"
     CDS             2194..2679
                     /locus_tag="VF272_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7259373.1"
                     /translation="MQASNPRKDLMPKLTDEVLRRLTDPALDVIRQARQVAGDLNHPY
                     ARPEHLMLGLLLQPTSVAAEVLRASGVTPEAYRTQIIERTGDGHAGEADNTPLSPRTQ
                     RVLEMALGMEPSIQAVGTEHLLVCMIRQGGEAIAILQELGVDVRQIRHGLESMIYATR
                     R"
     gene            2947..>3201
                     /locus_tag="VF272_00390"
     CDS             2947..>3201
                     /locus_tag="VF272_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259374.1"
                     /translation="MSKRNAIRQLEKREVFRANWLIVLVLLLLGSTALTFWLTLHITS
                     PWDPYGFKCLMAAPSAGNEDEDEDDENWRRHRGKDPKPQPP"
BASE COUNT          743 a          870 c          786 g          802 t
ORIGIN      
        1 cactggttct actcgccgct cgcagccttt gctaagtggc ccaccaacaa ccgcatacca
       61 ggcaaccagt tccgtcttga tttggttcca atttaaaatc taattatcgg taagctgtaa
      121 cgggatgggc atgataataa tttgactggg atattcaaat tttaccgaat aatcctcaaa
      181 aaagcctttt tgacccagta tcccggcata gtgacgtctt agtggttttt cggcaagctt
      241 agatgcatca attgcacgtt tcgcagaagc aacgaccgtt ttctcatcat ccgcaagagc
      301 aacccattta cccttgtatt gcttcataat gtttgaccaa tccattggcc gctcctttag
      361 cataagcatt atatacttct atcttagagt accgtaagca ttaatacaaa atacagtata
      421 tctattaaaa attacctttt agaaagataa atatttataa caagtgcata gattgtttga
      481 ctgtgttaaa gaaagttctt ttttagcatc aaggcgcaat tcatgattaa ccgtttcaac
      541 acatgggcgc atacattggt tgctgcggtt gggggttaaa gtggagtgga gtggagttga
      601 ggtgatgagg attagagttt taatctgatc ctggctgatc tggattttga agatccctat
      661 ataaattttt cttttgtttt tgcttaactc aataatatca taatggttat gtttatttta
      721 gtacaatatc ttggattaat aattgcaact cctaacaaaa gcttacggtt attttcttct
      781 ttttttatac ggttaactag catttcgatg gaggcctagc tggtagcctt agtaactgtg
      841 ccacccggca cgatggtcaa cccataggag gagtcttcat gacttcgacg caaacgcagg
      901 gcgccaagca gatgttcgac aacgccgtcg cgatgcagag cacgcagccc gaggctgcgg
      961 ccagcatctt cggcagcatc ggcgagaacg tggagggacc gctcggccag gctgcccgcg
     1021 agcagctgat cagcaccctg tgcgccctgg ggcgctccga ccaggccctg agggcgagcg
     1081 ccaagctcat gcaggagatc agggacaatt cgaagcagtc gtacttctgg gctcgcgttg
     1141 ccgtgcggtg ggccgagctc agcatcgcca acgggcgtga gtccgacgcc tcccaggttc
     1201 tggagcaggc cgagcgcatc ttcgagctca acggcgacta tcactacgtc ggcaaggtcg
     1261 ccgagctcaa ggccgcgatc tcgaccgcca agggcgacta cgacaaggcg gacatccagg
     1321 ccaagcgcgg gcagcggctg accgttaccc acgctccggc cgtcacaccc gggcgcacct
     1381 cgagcgcacc cgagctggcg ggcatcgtca gctccagcaa caactagcgc cacgggcgct
     1441 ttgaaacccc tggggggccg ttcggttccc caggggtttc acccaccttt cgaacaatac
     1501 gttttttacc cttcttgcaa caccgctagt acattgccgg ccggatcttt aaaccatgca
     1561 atgtccggac ccatgttggc tgccaggccg cgggcaatcc ccttatcgtc ggtcatgccg
     1621 ttgtagtgtt caaacttgat agctttgcca gctaactctt ctaccgcttt atcaatgtca
     1681 tctacagaaa agttcaatac ggtataggta gccggttcat gattgtcttt gggatagatg
     1741 aaaaggcctt tgccgccggt aatttttaat gacaggcctt gaggagtttc ttcggtttca
     1801 atacctaacg tgtcgccgta aaatttcttg gcggctgcaa ggtcgttaac tgaaaatcca
     1861 ctgaatgctg gggtgtcttt aaacatatgc tgcgctcctc ttttatttaa ttactagctt
     1921 gcttgttctg attccacaaa tttgattgat gccatcgctc gctaacccgg ccaccggttt
     1981 gcggccaagc ttgagcactg gcatgccgaa catattcgac ttggtaatac cgagttcttc
     2041 aaccgattca gccagccgct catagcttga atgcgttttc atggttatct ccctgactct
     2101 attgtagtat atccagtctt ttataaatct ggcgttttga cgattgataa tgctgaggcg
     2161 agtatataca atcccccact atggctcctt cacgtgcaag cttcgaatcc tagaaaggat
     2221 ctcatgccaa aactcaccga cgaagtactt cggcggctca ctgatccggc actggacgta
     2281 atcaggcaag ccaggcaggt ggcaggggat ctcaaccatc cctacgcccg gcccgaacac
     2341 ctcatgctcg ggctgcttct tcaaccaacg tccgtagcag ccgaagtgct gcgagcctcg
     2401 ggtgtaaccc ccgaggcata tcgcacccaa atcatcgaac gcactggtga tgggcatgcc
     2461 ggcgaagcag acaatacccc gctctcacct cgaacccagc gagtgctcga aatggcccta
     2521 ggcatggaac cctcgatcca ggctgtcggc accgagcacc tgctggtctg catgatcagg
     2581 caagggggcg aagccattgc catcctgcaa gagctcggag tcgatgttcg gcagatccgc
     2641 cacggactcg aaagcatgat ctacgcaaca aggcgctagc cgccggtaca accaatgaag
     2701 cccgcatcac gagtgcggct tcggtcgcac cggcgggttt tcgtatatgc tcatgttgct
     2761 ttcattaggc aactgcggta caagtttcac agacaagcac atcaaaccct cccccgcaag
     2821 gaaggatcgc gattgttttt ctcgttcttc tttttccagg ctgcgttctc tggtgccgac
     2881 ccgtccctct cgctgttcct agctttcata gtgctcgact tcatgatgtt tcatggaagc
     2941 catctgatgt cgaagcggaa cgccattcgc cagctggaga agcgagaggt gtttcgggcg
     3001 aactggctta tcgtccttgt gcttctgctg cttgggagta cggcgttgac cttctggctc
     3061 acactccata tcacctcgcc ctgggatccc tacggattca agtgcctcat ggcagcacct
     3121 tctgcaggca acgaggatga agacgaggac gacgagaact ggcgccggca ccgaggcaag
     3181 gaccccaagc cgcaaccacc a
//
LOCUS       DASVGB010000015         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_976382, whole genome shotgun sequence.
ACCESSION   DASVGB010000015 DASVGB010000000
VERSION     DASVGB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976382"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..557)
                     /locus_tag="VF272_00395"
     CDS             complement(162..557)
                     /locus_tag="VF272_00395"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX7259375.1"
                     /translation="MDINYVAVIVAAVVNMVIGAVWYSPILFAKPWMKATGKKMDDAV
                     NPGTGYALASVASIIIAMVMAHFIGRLAIESFLDGAMFGLTAWLGFVATTHGVNYIFE
                     GRSLNLFNINVGYSLVSFFLMGGLIAAMS"
     gene            672..1187
                     /locus_tag="VF272_00400"
     CDS             672..1187
                     /locus_tag="VF272_00400"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX7259376.1"
                     /translation="MDLEKPPHEERMQHRRRAIKSYRAKADKKRNLSARFADWLTSEF
                     GTVTFLALNAIWFAVWIAINTGLIQGIKPFDPFPFGLLTMIVSLEAIFLAIIVLISQN
                     RASKIAEIREEVDLQVNSITEGEVTKVIQLLMLLLEKNGINVKDDSELYEMLRPVRSA
                     DLERILEKQLS"
     gene            complement(1184..2134)
                     /locus_tag="VF272_00405"
     CDS             complement(1184..2134)
                     /locus_tag="VF272_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7259377.1"
                     /translation="MKYRGYGSAAVITASLLFSLDGLLRRQLYSLPPSVIVFWEHVLG
                     FLILAPIVFLSFKHFRKLTIKQWRAIIGVSFLSGALGTILYTSALTKIQFIPFSVVVL
                     LQQLQPIFAIAAAAVLLKEPLTKKFFGLSAVALAAAYFVSFPNLTVNWSTGNGTLIAA
                     LFAVGAAIAWGSSTAFSKYALKGTSSIHVTAARFGITPFFALGFVFVFGGGGSLLAIQ
                     PVQWLYLVAITFSTGLVALAIYYFGLKRIPASRATILELTWPLSAVVIGYIFLDQGLS
                     PTQILGMVVLMTTVYFIARDAQKLAAKEPDKVPESLSRKA"
     gene            complement(2473..3570)
                     /locus_tag="VF272_00410"
     CDS             complement(2473..3570)
                     /locus_tag="VF272_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7259378.1"
                     /translation="MTVPSNQLISFTRAVPPIAALMPTVQFEELATAAIRRDAITLQA
                     YGNQAGYPALRTQLATEYRVDESHILIGGEGSLQLLDLLAHDGEDSLVLVEQPSYDRA
                     IITFRRHGAQVVGIPMEDDGISVGGVEAAIAKLGAPRFVYLVPDSQNPTGVTLSGGKR
                     QAIIALAKQHDFTIIEDIPYRHLRYTGERQAMLRKLDNKHVVTISSFSKLIAPGLRVG
                     YMVAPAKIISDLVKLAEDTYVSPALLNQAIVSEFLLRGLLEDKIFQLIKLYRPRWQAA
                     MEAVAPLSGRQYPADGGFMIGVELPAGANTKNLVERAHNVGVGIAPGRLFYADNLNDA
                     DSEPDRFLRLPFCALSEAEFAEGIRRLAALL"
BASE COUNT         1042 a          960 c          837 g          871 t
ORIGIN      
        1 cctttaaggc tgtccaaaac cgccaactgc ttgctggtgg aaatctgcct ccagccgcct
       61 tggtcaatcc ggaatcaggc aatctttatt ggctggtcga tacggccgct gccggctagc
      121 actaaaatta tgtacaattc ataggggagc aattctgcgc cctagctcat agcagcaatc
      181 aggccgccca taagaaagaa gctcacaagt gagtatccga cgtttatatt gaataaattg
      241 agtgatcggc cttcgaaaat ataattaaca ccatgggtcg ttgctacaaa acctagccaa
      301 gcggtgagac cgaacattgc tccgtccaaa aagctctcaa tagcgagtcg tccaatgaaa
      361 tgggccataa ccatagcaat tattattgaa gcaaccgagg caagggcgta gcccgtgccg
      421 gggttaaccg catcatccat ttttttaccg gtagccttca tccagggttt ggcaaaaaga
      481 atgggcgagt accacaccgc accgattacc atatttacga ctgctgcaac gataaccgcg
      541 acataattga tatccatggc catgctcctc ttatttaatt tgatcgtact cctgccattt
      601 atctgatgtc aataaataaa aacgaggtaa cccctagtga cattatattc cgcttatgct
      661 ataaccagca tatggactta gaaaaacctc cgcatgaaga aagaatgcag caccgccgcc
      721 gggctattaa aagttatcgg gctaaagccg ataaaaagcg taatcttagc gcccggtttg
      781 ccgactggct aacctccgaa tttggcaccg ttacattctt agctctcaat gctatttggt
      841 ttgccgtctg gattgccatt aataccgggc tgattcaagg cattaaacca ttcgacccat
      901 tcccttttgg actgctcacc atgatcgtat ccttggaagc catcttttta gcgattatcg
      961 tactgatctc tcaaaatcgc gcatctaaaa ttgctgaaat ccgcgaagag gtcgacttgc
     1021 aggtcaattc aattaccgaa ggcgaggtca ctaaggttat tcaacttcta atgctcctgc
     1081 ttgaaaagaa tggcatcaac gttaaagacg attcggaact ctatgaaatg ttgcgtccgg
     1141 ttagaagcgc cgacctagaa cgaatcttgg aaaaacagct gagctaagcc ttccgggaga
     1201 gtgattccgg aactttatcg ggttcttttg ccgccaattt ctgagcatca cgagcaataa
     1261 aataaacggt tgtcattaat acgaccattc ctagaatttg agtcggtgat agcccttggt
     1321 ccagaaaaat atagccgatc actactgctg aaagcggcca ggtcagctcc aggatggtag
     1381 ctctggaagc cggaatccgc tttaatccaa agtaataaat tgccaaagca accagccctg
     1441 ttgagaacgt gatcgccact aaatacagcc actgcaccgg ttgaatcgcg agcagcgaac
     1501 ccccgccgcc gaacacaaaa acaaacccca gcgcaaaaaa cggagtaata ccaaaacggg
     1561 cagccgttac atggatgctt gaagtgcctt ttagggcata cttggaaaaa gcagtagaac
     1621 tcccccaggc aatagccgcg cccacagcaa acagtgcggc aatcaaggtc ccatttcccg
     1681 tagaccagtt cacggtaaga tttggaaaag aaacaaaata ggcagccgca agcgccactg
     1741 ccgataatcc aaaaaacttt ttagtcagcg gttctttcaa taatacggcg gctgcagcta
     1801 ttgcaaaaat cggctggagc tgctgtaaaa ggacgacaac cgaaaacgga ataaattgga
     1861 ttttggtcag ggcgctggta tataaaatag taccgagggc gccggacaaa aacgagacac
     1921 cgataatcgc ccgccactgt tttatcgtta attttctgaa atgcttaaag ctgaggaata
     1981 caatcggagc caatataagg aagcccagaa catgctccca gaacacaata accgaaggcg
     2041 gcaagctgta aagctgcctc cgtagtagtc cgtccaggct aaaaagtaat gacgcggtaa
     2101 taaccgccgc cgagccgtag cctcggtatt tcatgaatgc tctcctttct gctgcccgaa
     2161 tgcaacactc gcttgcaagc gagtcccgac tatacggtcg gcccatgagt ttcacatgat
     2221 catccctgaa tgtaatcaag gtcgacggct ttacgtccga ttggcgaatt tcacgccacc
     2281 caagagattt agttgttaat agaatagatg gtatcaatta gcatagccct ttagcaagtt
     2341 tcttgatcat aaaatctcac tctgacaagc ataatggctt gctgatatgt tattaacctc
     2401 cggataacct ttgtgcccgc agacaacttt tgcatgcaaa gcgtagatta aactaagacc
     2461 ccacttccct tactagagca gagcagccag ccgtcgaatc ccttcagcaa actcagcttc
     2521 gctgagcgca caaaatggga ggcgcaaaaa tcggtcgggt tcactatcgg catcattcaa
     2581 attgtcagca taaaaaagcc ggcccggcgc aatgccaaca ccgacattat gggcccgttc
     2641 gacgagattt ttggtattgg ctccggccgg cagctccacc ccaatcataa accccccatc
     2701 tgccggatac tgtctgccgc ttagcggcgc aaccgcctcc atcgctgcct gccaccgcgg
     2761 acgatacagc ttaattaact gaaaaatttt atcttcaagc aaccctcgca acaagaactc
     2821 agaaacaatt gcctgattca gtaatgccgg tgaaacatag gtatcttccg ccagcttcac
     2881 caaatcggaa atgatttttg ccggcgctac catgtagcct actcgcaaac cgggggcaat
     2941 caatttacta aatgagctga tagtcacaac gtgcttatta tctagtttgc gcaacatagc
     3001 ctgccgctca ccggtgtaac gcaaatgccg gtagggaata tcttcaatga tcgtaaaatc
     3061 atgctgctta gccagggcaa taattgcctg gcgcttgccg cctgaaagtg taacaccggt
     3121 gggattctgg gagtcgggca ccaaatacac aaatcggggt gcaccaagct tcgcaatagc
     3181 tgcttccacg ccgccgactg aaataccatc gtcttccatg ggtatgccaa ccacctgcgc
     3241 cccatggcgc cggaaggtaa taatggctct gtcatatgag ggttgctcaa ccagtaccag
     3301 actgtcttcg ccgtcatggg caagcaaatc taagagctgc aaggatccct caccgcctat
     3361 aagtatatgc gactcatcta cccggtattc tgtcgcaagc tgggtgcgta aagccggata
     3421 cccggcttga ttgccgtagg cctgtaaggt tattgcatcc cggcggatcg ctgccgtggc
     3481 caattcttca aactgaacgg ttggcattaa tgctgcgatt ggcggcacgg cacgggtaaa
     3541 actgatgagc tggttactcg gaactgtcat atattcaaca gtagcatacg atcaagcgaa
     3601 actctcctcc cttcagtggg ttacgttgtt tggatacgct attgagtttt ctattagtgc
     3661 tatacggaca gggctagcgg ggacttccaa ccagcgggtg ctggccgacc
//
LOCUS       DASVGB010000016        11728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1259600, whole genome shotgun sequence.
ACCESSION   DASVGB010000016 DASVGB010000000
VERSION     DASVGB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 11728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11728
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259600"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1149)
                     /locus_tag="VF272_00415"
     CDS             complement(181..1149)
                     /locus_tag="VF272_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX7259379.1"
                     /translation="MGIFNRNQKAEPGDIPSITSVPALVDTSVIIDGRIVDIAKAGFA
                     PGKLLVPRFVLAELQLIADSGDAMKRGRGRRGLEMLHSMREAGVDLEIIEDDYSDIKE
                     VDAKLVRLGRKLDANVLTTDYNLNRVASIEGVRVLNINELANAVRPVILPGEHMFTKI
                     VQPGKEKGQGVGYLADGTMIVVDSSERLIGQEVEVEITRVFQTVAGKMLFAVLVGGKP
                     APDQRTPEVAAADARPARSSGYQNNRGNQSKPAGRQQLKPETRQQAQHPQAQRGSHPG
                     PRPQTAKSQPTADDASVRSRRPKRVSPTQRLENSLMEMTSGEKDQA"
     gene            complement(1174..2508)
                     /gene="radA"
                     /locus_tag="VF272_00420"
     CDS             complement(1174..2508)
                     /gene="radA"
                     /locus_tag="VF272_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX7259380.1"
                     /translation="MAKTQTQYVCQSCGNTQSKWSGKCDSCSAWNSFVETVVSVRAAK
                     NPSAVAAKPEKLAEVATRNLPRIDTKIPEVNQVLGGGMVPGSITLLSGDPGIGKSTLV
                     LQLAAHIGSEHPVLYVSGEESANQIKLRADRLKVKADQLLLLSETNVGTVAATIEHGN
                     YKLVIIDSIQTMSAEALTGSAGSVGQITASAQILQRIAKQQHIAMILIGHVTKEGAIA
                     GPKVLEHLVDVVLYLEGDRYGSFKALRGIKNRFGSTSEVGIFEMQENGLIPVPNPSQA
                     LLAERQEAPGSVVLATVEGSRPILVEVQALVAKSVFGYPKRTASGFDLNRLNVLVAVL
                     AKRAGLNLSDQDIYVNVIGGLKITEPAADLAIILAIASAFHNAPVAHNLIAFGEVGLS
                     GEIRSVSNVSGRLAEAKKLGFRYAVGPSKIETKGISRVKTIAEAVAAAIIKP"
     gene            complement(2588..3616)
                     /locus_tag="VF272_00425"
     CDS             complement(2588..3616)
                     /locus_tag="VF272_00425"
                     /inference="COORDINATES: protein motif:HMM:NF010613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HEX7259381.1"
                     /translation="MQLIRQFTSKYPALRIYGLTLLVSLFACFAVGIKLGLAAFITTI
                     ILAVLEISLSFDNAVVNAKVLKTMSEWWRKIFLTIGILIAVVGVRLLLPVLLVSIFAH
                     IDFGTVVHLALNDSEKYGEMLHHIHPEIAAFGGIFLLMLFLDYIFGDREILWLRHIEL
                     PLIKLGRFKRLDVVIALLSLLGAVSLVDDAHASSMLISGIVGAVIYIVIKSVSDFFEH
                     SRSIQNMAKAGLIGFIYLEVLDASFSLDGVLGAFAITSDIVVIMVGLGIGALWVRSMT
                     LHLVQTDTLTRFRYLEHGAHYAIGVLAVLLLVSASQEISELITGLVGFCIIIAALVDS
                     IRFNRRAA"
     gene            complement(3752..6229)
                     /locus_tag="VF272_00430"
     CDS             complement(3752..6229)
                     /locus_tag="VF272_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7259382.1"
                     /translation="MSVEFGPQFDRFTENAKQSLENADAIARGMASSYIGTEHILLGV
                     LQQEGSIGAKILRSAGVTSDRAQDWVSNSQNVFARSSHKGLSETAKTTLTLSWRIARE
                     FGQPYSGTEHILFALLSQKNARANAILKEMHVDPAIVRAELENYLSNQQFFTGEDSQR
                     TQGKKAKTKTPALDGFGVDLTVKASKGELDPVVGREIQISRVVTILNRRSKNNPVLIG
                     EPGVGKTAIVEGLAQRIVAEEVPEMLQSKRIVMLDLASVIAGTKYRGEFEERLKKLLD
                     ETKANKDVILFIDELHTVVGAGAAEGAIDAANILKPALSRGEIQVIGATTLDEYRKHI
                     ERDAALERRFQPVQVPEATVEETIQVLHGLKERYEEHHRVKITDAAIEQAAKLAERYV
                     ADRFLPDKAIDLVDEAASLVRIKRGAISKPLRAMQKQLAEVQIEIEESVNDQDFEQAA
                     RLKTRASVLEQKLAALRLKEGSADNADLEITAEDIAQVISMMTGIPLMRLVDAEMINL
                     VKLDERLSQRVIAQSEAVSSVAKAIRRSRTGISDARKPIGSFIFLGPTGVGKTELARV
                     LSAEMFNSREAMIKIDMSEFMEKHNVARLVGAPAGYVGYEEGGQLTEQVRRKPYSLIL
                     FDEIEKAHPDVFNMLLQILEDGYVTDAKGRRVDFRNTIIIMTSNVGAEVMYNQAKLGF
                     QVETRKEELELEAQHERVKEQVVESLKKSFRPEFLNRVDDVIVFKPLSKLDIRQILEL
                     QLSDLSARIGEQGLSLRVSDTAKDLLIEKGYNVDQGARPMRRAIQDLMEDPLATGVLK
                     NEFKEGDTVSVTAKEGKLSLKALTPHA"
     gene            complement(6432..7655)
                     /gene="nusA"
                     /locus_tag="VF272_00435"
     CDS             complement(6432..7655)
                     /gene="nusA"
                     /locus_tag="VF272_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7259383.1"
                     /translation="MNTQFLAAINQIADEKGLPREIIVEAVESALAAAYKKDYGDKDQ
                     EARVKLNDDTGAIQVFVSKEVVADDTPEEDCNDHLHIRLKDAKKIDKNATLGTEEEPA
                     AVEMEVDPGEGFGRVAAQTAKQVIIQRLREAEREIIYNEYQDKEGTMINGTVQRVDGN
                     LTYVDIGRTNGVLFASEQIPGEHYYPGQRVKVYLVRVEQTARGPQIVISRAHPEMVRR
                     LFELEVPEIETGTVEIKNVAREAGTRSKVAVQSNSDSVDPVGTFVGGRGARVQAVMGE
                     LGEEKIDIILYSDHPETYIINALSPTKVVSVELDETEHKAKVRVPEDQLSLAIGKPGQ
                     NVRLAAKLTGWNIDILSADESRVEAAADAAVEEEPAKRKKKQDLESSLIEAVNQQSQS
                     ASGENSEPVEAEKTD"
     gene            7890..8351
                     /locus_tag="VF272_00440"
     CDS             7890..8351
                     /locus_tag="VF272_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259384.1"
                     /translation="MHPPRTQCLPVKSLWSVLWFSVSKFRETYLVLALGTVSTIVVVL
                     TFWEPATFGGPSPTLLFLCLPVAMFVGTLLTGHRMSKATALIGENIATAKELLWAFVI
                     VGAVVFLFGAMTSGGIDWSTNLLATFAACFLGNMAATDAMHKRDGATTSSG"
     gene            complement(8566..8790)
                     /locus_tag="VF272_00445"
     CDS             complement(8566..8790)
                     /locus_tag="VF272_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259385.1"
                     /translation="MTYYCSECVANWWPYMAKDGCCPQCGGGTSRRQEPASDDVDELY
                     RAASAETAKRDAYARFEAYYAEREREMQAA"
     gene            complement(8973..10778)
                     /locus_tag="VF272_00450"
     CDS             complement(8973..10778)
                     /locus_tag="VF272_00450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX7259386.1"
                     /translation="MQSINTAKTTWDLSPLLTGDDDPRIHEYRKELSAAATLFADTWR
                     ERTDYLERPDVLLDALNEFNELESLKANGREYYYFGLRSSVDAIDPKLKARENQAIEF
                     LQDISNQLQFFWLNIGTIDEKLQSAFLTHKGLASYHHWLEQQFRSAKHRLSEAEEKIM
                     LLKSTPAHSLWVQMTSDMLAKDEREVLTEEGKTKIATEEEVLSLLTSKQKKVRDVAVK
                     AMNAMLDDHADVAVTELNAILGNKKIDDQLRRYERPDAARHHSDDIDSGVVDALIAAV
                     SDRYDIAHRYYRMRAKLVGLKKLAYHEKTIEYGSDNVECKYPEASRLVHEVFIGLDSE
                     FADIYAEFLANGHIDAFPRKGKRGGAFCAHHGIAQPTYVMLNHTDKLRDVMTIAHEMG
                     HAINNELVRKTQKAINFGTPMATAEVASTFMEDFVVERLLKEADDEAELSLRIRQLDD
                     TIATIFRQIAFYRFEQDLHETFRAQGYLAQEEIGALFIKHIDAYLGPIFGGSKDAANW
                     WMYVPHFRSFFYVYSYASGLLISKAMQAKVRQDKSFIKEVKIFLSAGLSKSPQAIFAD
                     MKIDITDKAFWKNGLEEIDRLLIDTVKLAKKLGKI"
     gene            complement(10797..11150)
                     /locus_tag="VF272_00455"
     CDS             complement(10797..11150)
                     /locus_tag="VF272_00455"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX7259387.1"
                     /translation="MSVESGKQAEALAADWLAGRGFQILDRNWRTRWCELDIIADQGG
                     VIHIIEVKYRRRSDFGSGFEYITPNKAGRLQRGALMWLKAHRQSNSSFQIDVIAITGA
                     TKPENVTYLPNALGW"
     gene            complement(11140..>11728)
                     /locus_tag="VF272_00460"
     CDS             complement(11140..>11728)
                     /locus_tag="VF272_00460"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7259388.1"
                     /translation="LLWDQGHDLVIGIDEVGRGCLAGPLTVAAVGLPKQHRIRGIRDS
                     KWLTPKEREQAARQIRMSAAVIGIGWASHSEIDELGLTSALGLAGRRALLRLPRKSAI
                     ILDGKHNYLYDAYAVTTQVKADQTCKAVAAASVIAKVARDHYMELVHHLHPQYGFNTN
                     RGYGSARHLSELAASGPSPFHRRSFNPLREALIVG"
BASE COUNT         2814 a         3175 c         2692 g         3047 t
ORIGIN      
        1 taagttagtt gttacaagaa atgttgggcc ggcaattcca gcaaactcaa caagcccggc
       61 aacaagccga acctatgcca gcggctttgg tgtaccggga atttatcagt ggcatgttga
      121 atcggccgga gatgaaacac ccacctatac ctttaccatt accccgtaag ttttaggctc
      181 ttacgcttgg tctttttcgc cgctagtcat ttccatgaga ctgttttcca gtcgctgcgt
      241 cgggcttacc cgtttaggac gacgtgagcg tacagaagcg tcgtcggccg taggttggct
      301 tttagcagtt tgcggacgcg gtcctgggtg cgaacctcgt tgagcttgcg ggtgttgggc
      361 ttgctgccta gtctcaggtt tgagttgctg gcgcccagct ggctttgatt ggtttcctcg
      421 attgttctga taacctgaag atcgtgcggg acgagcatca gcggcagcaa cttcaggagt
      481 tcgctgatcc ggagccggtt ttcctcctac caagactgca aacaacattt tcccggccac
      541 tgtttggaat acgcgggtta tttctacctc tacctcctgg ccaatcaaac gctcgctgga
      601 atctactact atcatggtgc catcggccag ataaccgacg ccttggcctt tttctttacc
      661 gggctgcaca attttagtaa acatgtgttc tccgggaaga attaccggac gaaccgcatt
      721 tgctagttca ttgatgttta atacccgaac gccctcaata ctggccaccc ggtttaagtt
      781 gtagtcagtt gttagcacat tggcatcaag cttgcggcct aagcgtacta gcttggcatc
      841 cacttcttta atatcactgt aatcatcttc gataatttct aaatccacac cggcctcgcg
      901 catactgtgc agcatctcca ggccgcgccg gccgcgtccg cgtttcatag catcaccgga
      961 gtcggcaata agctgcaact cagctaaaac gaaccgtggc accagcagtt ttcccggcgc
     1021 aaacccggct ttagcaatat caacaatgcg gccatcgata ataaccgatg tatctactaa
     1081 cgcgggtact gaggtaatcg acgggatatc acctggttca gctttttggt tacgattaaa
     1141 tattcccata gggtataaat ccttttataa ctattatggt ttgataatgg ctgcggcaac
     1201 cgcctcggcg atcgtcttaa cccggcttat gccctttgtc tcgattttgc ttggtccgac
     1261 agcatagcga aagcctaatt ttttagcttc agcaaggcgt ccggagacat tactgaccga
     1321 gcggatttca ccacttaaac ctacttcacc gaaagcgata agattatgag cgaccggcgc
     1381 attatggaag gccgaggcta tcgccaaaat aattgctaga tcagcagcgg gttcagtaat
     1441 tttcaatccg cctataacat taacataaat gtcttgatca gacaagttta gaccagctcg
     1501 tttcgctaga accgcaacta gtacattgag tcgatttaaa tcgaagccgg atgccgttcg
     1561 cttgggatac ccaaaaacgc tttttgctac cagagcttga acctcaacca gaatgggtcg
     1621 actaccttca acggttgcca gtacaaccga ccctggagct tcttgtcgct cagctaataa
     1681 agcttgggat ggattgggaa ccggaatcaa gccgttttcc tgcatctcaa aaataccaac
     1741 ctcactggta gagccaaatc gatttttaat gccgcggaga gctttgaatg acccataacg
     1801 gtcaccttca aggtataaaa ccacatcaac caaatgctcg agcaccttag gcccagcaat
     1861 ggcaccttct tttgttacgt ggccgataag gatcatggcg atatgctgtt gcttggctat
     1921 tcgctgcaat atctgggcgc tggcggttat ttggcctaca ctaccggcgc tgccagttaa
     1981 agcctcagcc gacattgtct ggatggaatc gatgataaca agcttgtaat taccatgttc
     2041 aattgtcgcc gcaacggtgc cgacattggt ttcgctcaac agtaataact gatcggcctt
     2101 aaccttcaac cggtcggccc gcaatttaat ttggttagcc gactcctcgc cgctaacata
     2161 caacaccgga tgctcagacc caatgtgtgc cgctaactgt aaaactaatg tagacttacc
     2221 aatacccggg tcaccagaaa gcagcgttat ggagccaggc accatgccgc ctccgagtac
     2281 ctggttaacc tcgggaattt tagtatcaat ccgcggcagg ttgcgcgtag caacctcggc
     2341 cagcttctcc ggtttggccg caaccgctga agggttttta gcggcacgca ccgacactac
     2401 cgtttcaaca aatgagttcc aagcgctaca gctatcacat ttaccggacc atttagattg
     2461 tgtattgccg caggattgac agacatactg agtttgagtt tttgccatat aattcctttt
     2521 ttcagacgag catcatcttc tcatttgatt ataccgaaca taatacgaac atgcaatcgc
     2581 taggtaatta cgcagcccga cggttaaagc gtatggaatc taccagagca gcaataataa
     2641 tgcaaaaacc tacgagcccg gtaatcaact cggatatctc ttgactagcg cttacaagca
     2701 gcagaaccgc aaggacgcca atcgcgtagt gagctccgtg ctccaggtag cggaatcgcg
     2761 ttagcgtgtc ggtttgcacc aagtgaaggg tcatgcttcg cacccacagt gctccaattc
     2821 ctaatccaac cataataact acaatatcgc tggtaatggc aaatgcccct aaaaccccgt
     2881 ccaagctaaa gctggcgtcc aagacttcaa ggtaaataaa tccgatcaaa ccggctttgg
     2941 ccatattttg aatcgagcgg ctgtgttcga aaaagtcgct caccgattta atcacaatat
     3001 agataacggc gccgacaata ccggatatca gcatgcttga agcatgtgcg tcatcaacca
     3061 ggctaaccgc ccccagcagg gacagcaggg ctattactac atctaaacgt ttaaagcgac
     3121 ccagttttat caacggcagc tctatgtgac gcaaccatag gatttcacga tctccaaaaa
     3181 tataatccaa gaacagcatt aaaaggaata tcccgccaaa tgcagcaatt tccggatgaa
     3241 tatgatgcaa catctcgcca tacttttccg aatcatttaa ggctaagtgc accacggtgc
     3301 caaaatcgat atgcgcaaaa atcgaaacca acaaaaccgg cagcagcagc cgcacgccga
     3361 ctaccgctat taggatacca atcgtgagaa atatcttgcg ccaccattca gacatggttt
     3421 ttagcacttt ggcgttcacc actgcattgt caaagctcag gctgatttcg aggacggcta
     3481 aaataatcgt ggttataaat gcggccagac ccagcttaat gcctaccgca aagcaagcaa
     3541 aaaggctaac cagtaaagtc aggccgtaaa tccgcaaagc ggggtattta gaggtaaatt
     3601 ggcggattaa ctgcataggt atgtatgaag tttaccactt atcatcatag ctgctacaca
     3661 atgctggaat tatgtacgac cgactgtgca cctgcgtcgg taaggccctt gctgtcggga
     3721 gtgaattgtc ctggcactgt gtttgccacg actaggcatg aggtgtcagc gccttcaagg
     3781 atagcttgcc ttctttagca gttacggaaa cggtatcgcc ttctttgaac tcgttcttta
     3841 ggacaccggt ggctagcgga tcttccatta agtcttgaat ggcccgccgc attggacgcg
     3901 cgccttgatc aacgttataa cctttttcga tcagcaggtc tttagcggta tcggaaaccc
     3961 gcaaactgag tccctgctcg ccaatacggg cactcaggtc ggatagctgt agctccagaa
     4021 tttgacgtat atctaacttg gatagtggct taaagacaat cacatcatcg acccggttca
     4081 aaaactccgg tcggaacgat tttttcaacg actcaaccac ttgctctttg actcgctcgt
     4141 gctgggcttc taactccaac tcttctttac gtgtctctac ttgaaatcca agttttgcct
     4201 gattatacat aacttcggcg ccgacattgc tggtcataat gatgattgta ttgcggaaat
     4261 ccacccgccg acctttcgca tcggtaacat agccatcctc taaaatctgc agcagcatat
     4321 taaatacatc gggatgggct ttttcaatct catcgaacaa gatcaggctg taaggcttgc
     4381 gccgaacttg ctcggttagc tggcccccct cttcatagcc gacataaccg gccggagcgc
     4441 caaccaatcg ggcgacattg tgcttttcca taaactcgct catgtcgatt ttaatcatcg
     4501 cttcgcggct attaaacatt tcggcgctta gaacccgggc taattcggtt ttaccgacac
     4561 cagtcggacc caaaaagata aatgatccaa ttggcttgcg agcgtcacta attccggtac
     4621 ggctgcggcg aatggccttg gcgaccgaac tcactgcttc agactgcgca atcacccgtt
     4681 ggcttaaacg ctcatcaagc tttactaaat taatcatctc ggcatcaacc agccgcatca
     4741 acggtatgcc ggtcatcata cttatcactt gagcgatgtc ttctgcggta atttcgagat
     4801 cggcattgtc tgccgagcct tctttcaggc gcagtgccgc caatttttgt tccagtacac
     4861 tggcacgggt tttaagacgg gctgcctgtt cgaaatcttg atcattaacc gactcttcaa
     4921 tttcaatctg gacttctgcc agctgttttt gcatagcccg aagcggttta ctgattgccc
     4981 cgcgcttaat tcgcaccagg gaagctgcct catctacaag gtcaatggcc ttgtcgggaa
     5041 gaaaacggtc cgcgacgtaa cgctctgcca gctttgctgc ttgctcaatt gcggcatcgg
     5101 taatcttaac ccggtgatgc tcttcataac gctctttcag gccgtgaaga acctggattg
     5161 tctcctcaac cgtagcttca ggtacctgaa ccggttggaa gcggcgctca agggcggcat
     5221 cgcgttcaat atgcttgcgg tattcatcca aggtggtagc gccgataact tggatctcgc
     5281 cgcggcttaa tgccggcttt aaaatatttg ccgcatcaat tgcgccttca gccgcaccgg
     5341 caccaaccac ggtatggagc tcatcaatga ataagataac gtccttgttt gccttggttt
     5401 cgtccagcag cttctttaag cgctcttcaa actcgccgcg atatttggta ccggcaatta
     5461 cgctcgccaa atccagcatt acaatgcgct tgctttgcag catctctggt acttcttcgg
     5521 ccacaatgcg ttgagccaaa ccctcaacaa tcgcggtttt accgaccccc ggctcaccta
     5581 ttaataccgg gttgtttttg gaccgccgat ttaaaatcgt taccacgcgg ctaatttgaa
     5641 tctcgcggcc aaccactgga tcgagttcgc ccttagaagc tttaaccgta agatcaacac
     5701 caaaaccatc aagagccggt gtcttggttt tagctttttt gccttgtgtt cgctggctat
     5761 cttcgccagt aaagaactgc tggttactca aatagttctc cagctcggca cggacgatag
     5821 ccgggtcgac atgcatttct tttaaaatag cgttggcacg tgcattcttt tggcttaata
     5881 gcgcaaaaag aatatgctca gtaccggagt aaggctgacc aaactcgcgg gcaatccgcc
     5941 atgaaagcgt taatgttgtc ttagcggttt cacttaaacc cttatggctg ctgcgggcaa
     6001 aaacattctg agaattactt acccagtcct gtgcgcgatc ggaagttacc ccggcgctgc
     6061 gtaaaatttt agccccaatc gaaccctctt gctgcagcac gccaagcaga atatgctcgg
     6121 tgccaatata gctggaagcc atgccccgtg caatggcatc cgcattctct aaactttgtt
     6181 tagcattctc ggtgaatcga tcaaactggg gtccaaattc tacgctcaat gaaagctcct
     6241 ttccttctat accagaactt agtagttcta ttttagcact ctacccccgt gagtgccaag
     6301 ttgttttcat tccacccgcg aagggtacgg tatgatctag gaaaaggcgg aataacttca
     6361 tagtcataat ggcattacaa ctacacctct agtatatcac cagaagttca aatccaatct
     6421 aaatggcgaa atcagtcggt tttttcggct tcgacaggct cggaattttc acctgaagcc
     6481 gattgcgact gttgattaac cgcctcaatc aatgagcttt ccaggtcttg cttcttcttc
     6541 cgtttggccg gctcttcttc tactgcagca tcggcggcgg cctctacccg cgattcatcg
     6601 gcacttaaga tgtcaatatt ccaaccggtc agcttagctg ccaaacgtac gttttgcccc
     6661 ggcttgccaa tcgccaagct gagttgatcc tccggtaccc gaaccttggc tttatgctcg
     6721 gtttcatcaa gctcaacgct cactaccttg gtgggagaaa gggcattaat aatgtaggtt
     6781 tcaggatggt cgctgtagag aatgatgtca attttctctt cgcctaattc tcccataacc
     6841 gcctgcacac gggcaccgcg gccgccaacg aaggtgccaa ccggatctac gctatcggaa
     6901 ttcgattgaa cggctacctt actgcgagtt ccggcctcgc gtgcaacatt tttaatttca
     6961 acggtaccgg tttcaatttc cggcacttcc agctcaaaca accgccgcac catttccgga
     7021 tgggcgcggc ttataacaat ctgcggtccg cgggccgttt gttcaactcg taccaaatat
     7081 acttttacgc gctggccagg gtaataatgc tcgccaggaa tctgttccga agcaaacaaa
     7141 acgccgttgg tacggccaat atcaacgtag gtcaggttcc cgtccacccg ctgcaccgta
     7201 ccattaatca tcgtaccttc tttatcctgg tactcattat aaatgatctc ccgctcggcc
     7261 tcgcgtagcc gctgaataat gacctgtttg gcggtttggg cggctacgcg gccaaagcct
     7321 tctcccggat caacttccat ctctaccgca gccggctctt cctcggtgcc aagcgtggca
     7381 ttcttgtcga ttttcttagc atctttcaag cggatgtgaa gatggtcgtt acaatcttcc
     7441 tccggggtgt catcagccac cacttccttg ctaacgaata cttggattgc tccggtatca
     7501 tcattgagct ttactcgggc ttcttgatct ttatcaccat aatccttctt atacgcagct
     7561 gccaaagccg attcaaccgc ctcgacgatg atctctcggg gtaagccttt ctcatcagca
     7621 atttgattga tggcggccaa aaactgggta ttcatatggg ttcctattct attgttatta
     7681 ggctgggatt caaaaaatgc cggcctgctg gccgacgttc tatcaacgat tataccagcc
     7741 tcaataccgc ttgtaaacta catgccaaat tgacttttat agcttttatg ctattatact
     7801 tgcgacaaag acgttgactg ctcgctcaag ttagacttgg gctgtcttcg cttcgtcgat
     7861 ccgttcctca atccaaggag tgatgctgta tgcatccgcc ccgcacgcag tgcctgccgg
     7921 tgaagtcgct ttggtccgtc ctgtggttct cggtctcgaa gttccgcgag acctacctcg
     7981 tcctcgcgct cggcacagtc tccaccatcg tcgtggtgct caccttctgg gagcccgcca
     8041 ccttcggcgg cccgagcccg accctgctgt tcctctgcct cccggtcgcg atgttcgtcg
     8101 gcacgctcct cacggggcac cgcatgtcga aggcaacggc tctcatcggc gagaacatcg
     8161 cgaccgccaa ggagctcctg tgggccttcg tcatcgtcgg cgcggtcgtc ttcctcttcg
     8221 gcgcgatgac cagcggcggg atcgattgga gcaccaacct gctcgcgacg ttcgcggcct
     8281 gcttcctcgg gaacatggcc gcgaccgacg cgatgcacaa gcgcgacggc gcgacgacca
     8341 gcagcggcta acgcttcggc gccggcaacc tgcccacggc tgcccttcgg ggcggtcgtg
     8401 ggcaggaccc acaagcaata ataattttag cttaaaaaaa tataattacg gtccctgcca
     8461 ggaaggaaac ggcacagagg ccgaatcctt cctggagttg tcggggattc gcaggaacga
     8521 acgaacgagg aacgaagcca gacatgggca cactggcggc gaacctcatg cggcttgcat
     8581 ctcgcgctcg cgctctgcgt agtaggcctc gaaccgggcg taggcatcac gcttggcggt
     8641 ttcggcgcta gccgctcggt acagctcgtc gacgtcgtcg ctggcaggct cttggcggcg
     8701 actggtacca ccgccgcact gcggacagca gccatccttg gccatatacg gccaccagtt
     8761 cgcaacgcat tcactgcagt agtacgtcac tttcgatcac ctccagtgat cagtttgaag
     8821 ggttctcgtg cctgggtagg cacatcgagt gaagccctag ggtgcgaaag tacttcgtta
     8881 ttatgtctcc ggacaccatt atcatagtcc ctatgtacaa ttttcacaaa caaaaatcaa
     8941 aaatgacttt gaaatttaga attatcgcta gattaaattt tgcctagttt ctttgccagt
     9001 tttaccgtat caattaacaa tcgatcaatc tcctctaaac cgtttttcca aaaggcttta
     9061 tcggttatat caattttcat atcggcaaat atagcttgag gtgacttgga caggcccgcc
     9121 gaaagaaaga tttttacttc cttaatgaag cttttatcct gtcgaacctt ggcctgcatc
     9181 gccttggata tcaataatcc gctggcatag gaataaacat aaaagaacga acgaaaatga
     9241 ggtacgtaca tccaccagtt tgcggcgtcc tttgaaccac caaatatcgg gcctaaatag
     9301 gcgtcaatgt gctttataaa aagtgcgcca atttcttctt gggccagata accttgggct
     9361 cggaatgtct cgtgcaggtc ttgttcgaag cggtaaaagg caatctggcg aaaaattgtt
     9421 gcaatggtgt catccagctg gcgaatccgc aatgatagct cggcttcatc gtccgcctct
     9481 ttcaggagcc gctctaccac aaaatcttcc atgaaggtgc tggctacctc ggcggttgcc
     9541 atcggagtac cgaagtttat cgccttctgc gttttgcgaa ccagttcatt attgatagcg
     9601 tgtcccattt cgtgggcaat tgtcataaca tcgcgcagct tatcggtatg gttaagcatt
     9661 acatacgttg gctgggctat gccatgatgg gcgcaaaagg cgccgccgcg cttgccttta
     9721 cgcggaaaag catcaatgtg gccattggca agaaattcgg cataaatatc ggcaaactct
     9781 gaatcgagac cgataaatac ctcatgtacc agccgagaag cttctggata cttgcattcg
     9841 acattgtcag atccgtactc gatagttttt tcatgatagg caagcttttt caggccaact
     9901 agcttggcgc gcatccgata gtagcggtgg gcaatatcat accggtcgga aaccgccgct
     9961 atcagagcat ctacgacccc gctatcgata tcatcggaat ggtggcgggc agcatcgggc
    10021 cgttcatacc tgcgcaactg gtcgtcaatt tttttattgc ccaatatagc atttagttca
    10081 gtcaccgcca catcggcatg atcatcgagc atggcattca tggccttcac cgccacatca
    10141 cgcacctttt tttgtttgct ggttaacaga gaaagcacct cttcttcagt ggcaattttg
    10201 gttttaccct cttccgtcaa aacttccctt tcatcttttg ccaacatatc gctggtcatt
    10261 tgtacccaca agctatgtgc aggtgttgat ttcagcaaca taatcttctc ttccgcctcg
    10321 cttaagcgat gtttggccga gcgaaactgc tgctccagcc aatggtgata ggacgctaaa
    10381 cccttgtgcg ttagaaacgc cgactgcagc ttttcatcta ttgtgccgat attaagccag
    10441 aagaattgaa gttgattgga gatatcctgc aagaactcaa tagcctgatt ttcccgagcc
    10501 ttcaatttag gatcgatggc gtcaacgctt gaacgcagtc cgaagtagta atattcgcgc
    10561 ccgttagctt tcagactttc aagctcgtta aattcattca gcgcatcaag cagcacgtcc
    10621 ggccgctcca aataatcggt tcgctcgcgc caggtgtcgg caaatagtgt ggctgcggcg
    10681 cttaattcct tgcggtattc atgaattctc ggatcgtcat ctcccgtaag cagcggcgaa
    10741 agatcccagg tggttttagc ggtattaata gattgcatga tacctagtat actccactac
    10801 cagcccaggg cgttgggcaa atacgtaaca ttttccggct tggtagcacc ggtaatggca
    10861 ataacatcaa tttgaaagga actattgctt tgccggtgcg ccttcagcca catgagagca
    10921 ccgcgttgca ggcggccagc cttgttggga gtgatgtatt caaagccgct gccaaaatcg
    10981 gatctccggc gatatttaac ctcaataata tgtatgaccc cgccttgatc ggcaataatg
    11041 tccagctcgc accaccgagt gcgccaattg cgatcgagta tttggaaacc tcttcctgcc
    11101 aaccaatccg cagccaacgc ttcggcttgc ttgcccgatt caaccgacaa tcagtgcttc
    11161 tctgagtgga ttaaaacttc gcctatggaa cgggcttgga ccggacgctg caagctcaga
    11221 tagatgcctg gccgagccat agccgcggtt ggtattaaag ccgtattgag gatggagatg
    11281 atgaaccagc tccatgtaat gatcgcgcgc aaccttagcg atgactgatg ccgccgccac
    11341 agccttgcac gtttgatcag ctttgacctg ggtggttaca gcgtaagcat catataagta
    11401 gttatgttta ccatctaaaa taattgcaga tttcctggga agcctcagga gtgcccgcct
    11461 tcccgccaag cccagggcgg atgttaagcc gagctcatca atctccgaat gcgacgccca
    11521 tccaatcccg attaccgctg cgctcatgcg gatttggcga gcggcttgtt cgcgctcttt
    11581 aggtgtcagc catttggaat ctctgattcc tcgtatacgg tgctgctttg gcaaacccac
    11641 agccgccacc gttaatggac cggccaggca cccccggcca acttcgtcga taccgattac
    11701 cagatcatgt ccttgatccc ataatagc
//
LOCUS       DASVGB010000017        13651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_281582, whole genome shotgun sequence.
ACCESSION   DASVGB010000017 DASVGB010000000
VERSION     DASVGB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 13651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13651
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281582"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /gene="ppcA"
                     /locus_tag="VF272_00465"
     CDS             <1..1406
                     /gene="ppcA"
                     /locus_tag="VF272_00465"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR02751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7259389.1"
                     /translation="AFFMFSELGVDEYKWDWEGKLVDESVMERLFSDYYDYFHDHPIG
                     HEKFITFRLPNPKTDTEFRLGRAFMTLLSSSVLANHFKLPPEPLFEVIVPMTETSREL
                     IAVEEAFDEIASLKHPLYRLNERTIKHIHVIPLFEDVDTIIRSDEIVQEFIDKHRQVF
                     GSPPTYIRPYIARSDPALNSGMAATVLATKIALSRYKRLAEENGIPMYPIIGSASLPF
                     RGGLTPYSVEAFAKEFSGIRTALLQSAFRYDYPKDDVIQAIKQLHDILPNSQPMEISA
                     ADESGLVEAIAVFEQAYRPSVEGLADTINHLATKLPRRRERVQHIGLFGYSRGVGGIK
                     LPRAIGFTAALYSLGVPPEFIGTGRGLKKVRAMGKLSLVEKYYLNLKADLLRAGKFIN
                     HGVLAKLAATNPAWAAIAEDIKEVEAYLDVKLEPETDEERKHHELTTQIFAHMTDEGD
                     TLTNLIESAARMRHSMG"
     gene            1418..2629
                     /locus_tag="VF272_00470"
     CDS             1418..2629
                     /locus_tag="VF272_00470"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7259390.1"
                     /translation="MLQTAEAFIKRAGEKLYLKPDEINALLQPNAEHQFDIVLKDGKT
                     FKGYRVQHSNARGPYKGGIRFHPEVNLEEVRALATLMSLKTAAVGLPLGGGKGGVAVN
                     PRELSAWELEEMSRQFVANLHPYIGPDKDIPAPDVNTNSMIIDWMVDEFEKRTGDTSK
                     ASFTGKSLGNGGSLGRDAATGRGGVIALRELLHHRGKKDERLTVAVQGFGNVGAFFST
                     VAAEDHPEWLLVAAGDSKSGLQSSNGLHAKKLSQYKTAGGSFKDYQQEGVERISNTEL
                     LGLDVDVLVLAALGDTITEANSDDIKARYILELSNAPINEAANDSLTSKGVIILPDII
                     ANAGGVVVSYLEWMQNRQDEHWTEQQVNQKLEKYMSEAVADLLKLAKEADVEMREAAF
                     MLALQRLTGQD"
     gene            2757..3416
                     /locus_tag="VF272_00475"
     CDS             2757..3416
                     /locus_tag="VF272_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7259391.1"
                     /translation="MHQRSLKAPSGLRNGRTFPNSRYRPNQSSGRRGGFQGSYINPTK
                     FVNKATAAADQPIFEPVHKFSDFGFSPELQRNLVNRNYSTPTAIQDGSITAIMEGKDL
                     IGLANTGTGKTAAFVLPIIQQLQHQRHQNRVLIMAPTRELAIQIDDEFRAFAAGLGLY
                     SAMCVGGVAIARQISQLARKPHVIIGTPGRLKDLVNRRALNLTKTDILVLDEADRMLD
                     M"
     gene            3513..3974
                     /locus_tag="VF272_00480"
     CDS             3513..3974
                     /locus_tag="VF272_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX7259392.1"
                     /translation="MDDLLHDPVLVSVRVSETSEHVEQDVIKAGSPQEKLTVLSELLR
                     KPEFDKVLIFGQTKRGVQNLADKLTKEGMPAEAIHGNKSQSQRQRALKAFKSNQVGVL
                     VATDVAARGLDIPNVSHVINYDQPNTYEDYIHRIGRTGRAGNSGHALTFIT"
     gene            complement(3992..4555)
                     /locus_tag="VF272_00485"
     CDS             complement(3992..4555)
                     /locus_tag="VF272_00485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7259393.1"
                     /translation="MFSVHSSYQEVITIINKGGVVVMPTDTIYGVVGSALQSETIERL
                     YALRQRDPLKPVIILIGSLSQLKSFHVSLEPLVLPVLEQLWPGPTSVVLPCPHPELAY
                     LHRGGRSLAFRFPKKPELLAMLAETGPVAAPSANPEGMAPATTIQKAQDYFNGRVDAY
                     VDGGTLVGSASRIVQMHPDGSYDVIRP"
     gene            4570..5169
                     /locus_tag="VF272_00490"
     CDS             4570..5169
                     /locus_tag="VF272_00490"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7259394.1"
                     /translation="MLGSNFYNQDSVMAAQSLLGCELIYTPAGGKSRGGLIVETEAYA
                     QSDEASHSFRGLTKRNAVMFGPGGYAYIYFTYGVHYCFNAVVGKPGYAEAVLIRALEP
                     TRGIELMQHNRGTVNLFNLCSGPGKLVQALGLRREHNGASLRTSELHINKRTSRPPIA
                     ASPRIGISRAVDKPWRFMIAESPWVTKHKLNKTAFAVQI"
     gene            5166..5738
                     /locus_tag="VF272_00495"
     CDS             5166..5738
                     /locus_tag="VF272_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX7259395.1"
                     /translation="MSRSVHRKLMVLLILLILSAFGVGINQQGKLALPKPNLTAPPGY
                     YPVTSVSDGDTIIVTIAGKPEIIRLIGIDTPETKDPRKPVQCFGKAASAHAQTLLDRK
                     VVRLEGDPANNDRDKYRRLLRYVYLPDGTLVNQYMIKNGFAFAYTLFPFTKMQEFRAA
                     EREARAANRGLWAGCEVSKEGEKSQTHDQP"
     gene            5826..7397
                     /locus_tag="VF272_00500"
     CDS             5826..7397
                     /locus_tag="VF272_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259396.1"
                     /translation="MSTSVWFPSVVTPTFLTRELLAVKGGQKASAGGVYDDLFLRDGA
                     RVAKAIAKHHPGAAIAVLLALAQFQGEQADASTNEFPGALPHQVHREFVGGMMSSPTL
                     IEHSARYASTWGVAMRGSSHCGHHFAIYNDSDGQCLFVIVLRRLMQLHPRLNVLGREY
                     RHWATNTTRSLAVAATEVLRFLVRMIHDSNLGLYEIPNTNPRQTSPSGALFDGFDAYF
                     HPAKDGIRPVNWRLVAYIHNQQLAYEALLCGAELFPDDRNVQLWLDTADQLRRNAVRL
                     FGLDSGTGFPPAAYDRDEFGRARPVHLESLASLELLEGRFLSGLLTQTEIVRMLVKWL
                     YSEAVMTAVGPRCQPLKYAPLEGEYCPYQGTPSVWAIMDWIVAEGLHNQGLDPLFHDL
                     ALLRMTAGLDRSGTAIESWPVHRETGAVCYRPHETTTRGDADLVMACSEVGQLRQAWS
                     ISSGLAALEAQAIGYRDVPGNSWQEQLCKEALSAVSHIFPAADNRPEILIHIDLERGR
                     ELKAKRVAQMGLAAH"
     gene            complement(7487..7879)
                     /locus_tag="VF272_00505"
     CDS             complement(7487..7879)
                     /locus_tag="VF272_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259397.1"
                     /translation="MEDETTVAAIHRLMTEMEETRAKIKAMRDNLKDVLEQNQEYKEL
                     QEELKELKTKRQQAKKLLEADRDYQTINSELEELKFKQKDLQEIMSHHLVTYYNETQD
                     TTIKDHEGETRQLILSAKVGKPEMTVNE"
     gene            complement(8110..9285)
                     /locus_tag="VF272_00510"
     CDS             complement(8110..9285)
                     /locus_tag="VF272_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7259398.1"
                     /translation="MSYQLSPDQIEALQTIGRWYRSGTQRYVTLGGYAGTGKTTMLGH
                     VRQALRDNDPDVKVAFCAFTGKAVRVLDQTLKRQGVSRKGDSLTTIHGLIYTTELGSD
                     GQVTGWRRRQTIKADLIMVDEASMVTEDIWKDLLSYKVPILAVGDHGQLPPIGTNFNL
                     MNEPMIRLEKVYRQAIDSPIVEVATLARTTGTIAPGRYGDEVVKYDRHSNELGAAMDE
                     LLEAWSPEWLVLCGYNHTRVRLNRELRARRGFETPNPEPGEQLICLRNNTAGKLYNGM
                     TGTLKWIQSVSGDVEATWWEAGIMIDDTERHCYLMREQFGSRETIAKVPPSPEPKVRG
                     DLFDFGYALTVHKAQGSSADTVLLFEERNKHMNDDDWRRWLYTAVTRAERQLIIIGD"
     gene            9418..9624
                     /locus_tag="VF272_00515"
     CDS             9418..9624
                     /locus_tag="VF272_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259399.1"
                     /translation="MTAVKRAMSDVQATTILGSEAAPIDKVRQLVAIGYEEEVADLMV
                     SGVQSGQHQMLYYEQLPQADYADD"
     gene            9662..10504
                     /locus_tag="VF272_00520"
     CDS             9662..10504
                     /locus_tag="VF272_00520"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX7259400.1"
                     /translation="MKYGLHISTAGNLSTTPGRAEAMTAAALQIFAGSPRTFKQPTYT
                     PEITDGFKQACTDLKLPSFIHMLYLTSYGSPDDKLRDSSIAAAKQTMRNADLLGVEGV
                     VTHLGSHKGEGTAAVIDRLRDALLDVVDGVETKLLLENSAGAGGNVGNSIEELALIYE
                     AVGRTPKIGFCLDTAHLFASGYDVRTDEGWKGVLDEFDAKIGLKNLGVIHLNDSKVDL
                     GSKRDRHENIGNGFIGEAGFKVILNEPRLDGMVGLLEVPGIDDKGPDKPNLDKLRELT
                     LSAL"
     gene            10501..11367
                     /locus_tag="VF272_00525"
     CDS             10501..11367
                     /locus_tag="VF272_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259401.1"
                     /translation="MIPVFERQTDTPLFPDMLWNRPAMKSRGGRLLVLGGQTNEFSLL
                     QAIYQMAETAGIGEVVVAMPDSLRRSAGKAGYGQFVPASSSGSLGKAALGEIVHLAGE
                     ADAVIIGANLTNNAETGMMIESLVRELDSRVIFTEEAFEILQFHPDFITGNPKALVVA
                     TMKGLFDLANHHKMPIAIRPNAGVIGKLEILEQIASISKCQYVVFDKEIMVAAEGELS
                     LTPLNTALSSLPAAPIGIAATFWVQQPGKPYEALTTAGYVLAAALQKDTTTPSYSNLS
                     KQVEAVLREQDQ"
     gene            complement(11586..11672)
                     /locus_tag="VF272_00530"
     tRNA            complement(11586..11672)
                     /locus_tag="VF272_00530"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11636..11638),aa:Leu,seq:caa)
     gene            11746..12957
                     /locus_tag="VF272_00535"
     CDS             11746..12957
                     /locus_tag="VF272_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HEX7259402.1"
                     /translation="MSQSEIVRFPRSSMPNRDNNPDAAKEARERIAGINSPYLAHAAP
                     AKEDLVGAIHPISPAANVQDGKPTADAPPFTISSSDDEPQPAPAAETTVSAQPATPEA
                     HDHLLPPAHGANALPTQPQAAQTQPTPTIPPPNPLEQLSSGVPKKKTSHSNRPRTVAQ
                     PKSLPSRFMPLIVGVITFVLVLLVVKAPILLNQLSYFTNDKPPAAQNAPAANVVAVSP
                     DPVITIPKINVSAPVIYTDNNAESIIQKHLESGVVHYAGTALPGTNGNSVIFGHSSND
                     WWEPGNYKFVFVLLDKLQPNDTFTVNYNSRQYLYQVTGSKVVQPTDLSVLNPTPEPTM
                     TLITCTPPGTSWQRLVVTAKQISPVAETEKAPAIDEEVAADGTLPGNAPGIGQRLSDW
                     WKGLTNVFTRE"
     gene            complement(12944..>13651)
                     /locus_tag="VF272_00540"
     CDS             complement(12944..>13651)
                     /locus_tag="VF272_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259403.1"
                     /translation="PPDLTFSPSNWRQLYWMSDQGLRRLDMGNNSISAVLASNVAGFT
                     FDGNRMLYVSHAEPTPSLWVLDANGETKRLADNLPVSQTYNLDFAHYIRTPMLAVVAV
                     DSRKTLLYRNLYHSPRPIELPAVAASAVFNGDGRFLLQFDESHSATYDLQENTTYSLP
                     PSVNAASGVAWLGNFHLLSNQSGSIMLSEYDGNYLNTLTKGGVLPAFVSGDTRSVIVA
                     SPNAAGAIHIRRVIIRE"
BASE COUNT         3360 a         3692 c         3513 g         3086 t
ORIGIN      
        1 aagccttttt tatgttttcg gagctgggtg ttgatgaata caagtgggac tgggaaggaa
       61 agctggttga tgaatcggtc atggagcggt tgtttagcga ctactacgac tactttcacg
      121 atcatcccat cggccacgaa aaatttatta ccttccgctt gcccaacccc aaaaccgaca
      181 ccgagtttcg gcttggacgg gcctttatga cgctactgtc gtcctcggtc ctggcgaatc
      241 attttaaact gccgccggaa ccgctatttg aagtaattgt acctatgacc gaaacatcaa
      301 gggagctgat tgccgttgaa gaggcttttg atgagatcgc cagcttaaag cacccgctgt
      361 accggctcaa cgaacgcact attaagcaca ttcatgttat cccgctgttt gaagatgttg
      421 acaccattat tcgctcagat gaaatcgtcc aagaatttat tgataagcac cggcaggttt
      481 ttggctcgcc gccaacctat atccgaccat acattgcccg ctccgatccg gctctaaatt
      541 ccgggatggc cgcaaccgta cttgctacaa aaattgcact ctcacggtat aagcgactgg
      601 cggaagagaa cggtattccg atgtatccaa ttatcggctc ggcctccctg ccatttcgcg
      661 gcggccttac cccgtattca gtcgaggcgt ttgctaagga gttttctgga atccgcaccg
      721 cattgcttca gagtgccttt cgctacgact acccgaaaga cgatgtcata caggcgatca
      781 aacaattaca cgatatatta cccaattcgc agcctatgga aatatcggcg gccgatgagt
      841 ccgggttggt cgaggcaatc gccgtttttg agcaggccta tcgcccttcg gttgagggcc
      901 tggcggacac cattaaccat ctggcaacca agctgccgcg ccggcgagag cgggtgcagc
      961 atattggttt gttcggctac tcgcgcgggg taggcggcat taagctgccg cgggcaatcg
     1021 gttttaccgc cgctctctac tcgctcggcg tcccaccgga gtttatcgga accggacggg
     1081 gacttaaaaa ggtacgtgcc atgggtaagc tatccttggt tgaaaagtat tatcttaatt
     1141 tgaaggctga tttgctccgg gccgggaagt ttatcaacca cggtgttctc gcaaaactgg
     1201 ctgctaccaa cccggcatgg gcagcgattg ccgaagatat taaggaagtc gaagcttacc
     1261 tggacgtgaa gctcgaaccg gaaaccgacg aagaacgtaa gcaccacgag cttaccactc
     1321 aaatttttgc gcacatgacc gatgagggtg atacattaac caacttaatt gaatccgcgg
     1381 cgcgcatgcg ccacagtatg ggataggaga aagcacgatg ctacagacag ccgaagcatt
     1441 tattaaacgg gcgggagaaa agctttatct taaacccgac gaaatcaatg cactattaca
     1501 accgaatgcc gagcaccaat tcgatatcgt cttgaaagac ggtaaaacct ttaaaggtta
     1561 tcgagtgcaa catagtaatg cccgcggacc atacaagggc ggtatccgct ttcaccctga
     1621 agttaacctg gaagaggttc gggccttggc taccctaatg agcttaaaga ccgcagcggt
     1681 tggtttgccg ctcggcggag gcaagggcgg cgtggcagtt aacccgcgtg agcttagcgc
     1741 ctgggaactg gaagaaatga gccggcagtt cgttgccaac ttgcatccgt atattggacc
     1801 ggacaaggac attccagcac cggatgttaa tactaattcg atgataatcg actggatggt
     1861 cgacgagttt gaaaagcgta ccggcgacac ctccaaggcc agctttaccg gtaaaagctt
     1921 aggtaatggc ggcagccttg ggcgcgatgc cgctaccgga cgcggcggcg tcattgcgct
     1981 tcgcgagctt ttgcaccacc gaggcaagaa agacgaaaga ttaacggtcg cagttcaagg
     2041 ctttggcaat gtcggcgcct ttttttcgac cgtcgctgcc gaggatcatc cggaatggtt
     2101 gttagtagca gccggcgact cgaaaagcgg gctgcaatct tcaaacgggt tacatgccaa
     2161 aaaactgagc cagtacaaaa cagccggcgg gagcttcaag gattatcagc aggaaggcgt
     2221 tgagcgcata agcaacaccg agttattagg acttgatgtt gatgttttgg ttttagcggc
     2281 gcttggcgac actattacag aagccaatag cgacgacatt aaagcccggt acatccttga
     2341 actatcaaac gctccgatca atgaagcagc caacgactca cttacaagca aaggggtgat
     2401 aattctgccg gacatcattg ctaatgccgg cggcgtagtg gttagttact tggaatggat
     2461 gcaaaatcgt caggatgagc actggaccga gcagcaggtt aaccaaaaac tggaaaaata
     2521 catgtctgaa gcggtagccg atttgctgaa gctggcgaag gaagccgatg tggaaatgcg
     2581 tgaagcagca tttatgctgg cattgcagcg attaaccggg caggactagc caaaacttct
     2641 cccctatgct ataattagct taattgctag atgataaatg ccgagccgct ctcttttctc
     2701 gttgtttgtc tatatgagcg agaaacacga gaacataatt agaaaggcat aattttatgc
     2761 atcagcgaag ccttaaggct ccgtccggac tacgcaatgg ccggacattt cctaattccc
     2821 gttatcgacc caatcaatca agcggtcggc gaggcggatt tcaaggaagt tatattaatc
     2881 caaccaaatt tgtaaacaaa gccacggccg cggcggatca gccaattttc gagccggtgc
     2941 ataagtttag cgacttcgga ttcagcccgg agctccagcg caatctggta aatcgtaatt
     3001 attcaactcc aaccgcaatc caagacggct caattacagc cattatggaa ggcaaggact
     3061 tgattggact ggctaacacc ggtaccggta aaaccgccgc cttcgtgctg cccatcatcc
     3121 aacagctaca acaccagcgc caccaaaatc gcgtactgat tatggcgcct acccgcgaac
     3181 tggcaattca aattgacgat gagttccggg cttttgctgc aggcctcggt ctgtacagcg
     3241 ctatgtgtgt tggcggcgtc gcaattgcgc gccaaatcag ccagctagcc cgcaaaccgc
     3301 atgtcatcat cggaacgccc ggacgactga aagacctggt taaccgccga gctctcaatt
     3361 tgaccaaaac cgacatctta gtcctcgacg aagccgaccg catgctcgac atgtgattcg
     3421 tccgcgatat tcgctttctg attgcccagc tgccagccaa gcgccaatca ctctgcttca
     3481 gtgccaccgt tactcccgat attaaggtcc tcatggatga tttactgcac gatccggtat
     3541 tagtgtcggt gcgagttagt gaaaccagcg aacatgttga gcaagatgtc attaaggccg
     3601 gttcacccca ggagaagctc acggttttaa gcgagctttt gcgcaagccg gagtttgata
     3661 aggtattaat tttcggccaa accaaacgcg gcgttcagaa cctagccgac aagcttacga
     3721 aagaggggat gcccgccgaa gcaattcatg gcaataagtc acagtcgcaa cgccaacgag
     3781 cgctcaaggc ctttaaaagc aaccaggtag gcgtattagt tgccaccgac gttgccgccc
     3841 gcggtcttga catcccaaat gtcagtcacg tcataaacta tgaccaaccc aatacctacg
     3901 aagattacat ccaccgcatt ggccgaaccg gccgggcggg caacagcgga cacgctctaa
     3961 cctttatcac ctaggctcat caatctatgg gttacggccg tattacgtcg tagctgccat
     4021 ccggatgcat ctgcacgatg cgtgaagcgg aaccaacaag cgttccgcca tcaacatagg
     4081 catcgacccg accgttaaaa tagtcttgcg ctttctgtat agtggtagct ggcgccattc
     4141 cttccggatt ggcactcggc gctgcaaccg ggcccgtttc agcaagcata gccagcagct
     4201 ccggcttctt cgggaaccga aacgccaagc tccggccacc ccggtgtaag taggcaagct
     4261 cgggatgagg acagggaagt acaacactgg ttggaccggg ccacagctgc tccaacaccg
     4321 ggagcaccag cggctccagt gaaacatgaa acgacttgag ttgcgaaagg ctaccaatta
     4381 aaataattac cggctttagc gggtcacgct gtctcaatgc atacaaacgc tcaatcgttt
     4441 cggactgaag ggcgcttccg accacgccat aaatcgtatc ggtgggcata acaaccacgc
     4501 cgcctttatt aataatggta ataacttctt ggtaagatga gtgtacggag aacatagggt
     4561 tattagtata tgcttggatc aaacttttac aaccaggata gcgtcatggc agcacagtcc
     4621 ctattaggct gtgagctcat ctatactccg gctggcggaa aaagccgagg cgggcttatt
     4681 gttgaaaccg aagcctacgc acaatccgat gaagccagcc attcttttcg aggcctcact
     4741 aagcgcaatg cggtgatgtt cggccccggc ggctacgctt atatctactt cacctacggc
     4801 gtacactatt gctttaacgc agtggtaggg aaaccgggct atgccgaggc ggtcttgatt
     4861 cgagcgctcg agccaacccg aggcattgag cttatgcaac acaatcgcgg cacggtaaac
     4921 cttttcaatc tgtgtagcgg tcccggcaaa ctggtgcaag ccctgggact acgccgcgaa
     4981 cacaacggcg caagcttgcg cacttctgaa cttcacatta ataaaaggac ctcccggccc
     5041 cccatcgctg ccagtccgcg cattgggatc agccgggctg ttgataagcc atggcgattt
     5101 atgattgccg agagcccctg ggtcaccaaa cacaagctca acaaaacggc gttcgcagta
     5161 cagatatgag ccgtagtgtt catcgcaaac tgatggtcct gcttatcctg ctgattctat
     5221 cggcattcgg ggtcggcatt aatcaacaag ggaagttggc tctgccgaaa ccgaatctga
     5281 cggcgcctcc aggctactat ccggtaacaa gtgtatccga cggcgacacc attatcgtta
     5341 ccatcgccgg caaaccggaa attatccgct tgataggaat tgatactccg gaaaccaaag
     5401 atccgcgcaa gccggtacag tgttttggca aggccgcatc tgcccatgct cagactttac
     5461 tcgaccggaa ggtagttcgg ttggaaggcg accccgcaaa caacgaccgc gataagtacc
     5521 ggcgtttact gcgctatgtc tacttgccgg acggaacgct cgttaatcaa tatatgatca
     5581 aaaacggctt cgcttttgcc tacaccttat ttccgtttac caaaatgcaa gaattccgag
     5641 cagccgaacg cgaagcccgc gcagctaatc gcggactgtg ggccgggtgc gaggtgagta
     5701 aagaaggtga gaagtcgcaa acgcacgatc aaccctagag gggatagccc gctgaagatt
     5761 ttaagcagca aatatgtaat aatcactagg ctcatgaccc tggcttcgaa atgaaatgag
     5821 agcacatgtc tacttcggtt tggtttccga gtgtagtaac cccaacgttt ctgactcgcg
     5881 agctactggc cgtcaaggga ggtcaaaaag cttcagcagg aggcgtttat gacgacctct
     5941 ttttgcgaga cggagcacga gtcgccaaag cgattgcgaa acatcacccc ggtgcagcta
     6001 ttgcggttct gctagcactt gctcagttcc agggcgagca ggctgacgcc tcaaccaacg
     6061 aattcccggg cgcccttccg catcaagtgc atcgcgagtt cgtgggcggg atgatgtctt
     6121 cgccgacgct catcgagcac tcagcccgct acgcttctac ctggggcgtt gcaatgcgcg
     6181 gcagcagcca ttgcggccac cacttcgcga tctacaacga ctccgacggg caatgcctgt
     6241 tcgtcatcgt cctgcgacgg ctgatgcaac ttcatccgcg gttgaacgta ctcggacggg
     6301 aataccggca ttgggccacc aacaccaccc gcagtctggc cgtggcagcc acggaggttc
     6361 tgcgcttcct cgtcaggatg atccacgact cgaatctcgg gctctatgag attccgaata
     6421 ccaacccccg tcagaccagc ccgtcaggcg ccctgtttga cggttttgac gcctactttc
     6481 accctgccaa ggatggaatc aggcctgtca attggaggct ggtagcctac atccacaacc
     6541 agcagctggc ctatgaggcc ctgctttgcg gcgccgagct attccctgac gatcgaaacg
     6601 ttcagctatg gctggatacc gccgatcagt tgcgccgcaa cgccgtacgg ctctttgggc
     6661 tcgatagcgg caccggcttc ccaccggcgg catacgatcg ggacgaattt ggtcgagcgc
     6721 ggccggttca tcttgaaagc ctggcctcgc tggagctact ggagggtcgt ttcctatctg
     6781 gcctgctgac gcaaacagaa atcgtgcgca tgctggtgaa gtggctctac agcgaagctg
     6841 tgatgacggc cgtgggaccg cgctgccaac cgctgaagta tgcgccactg gaaggcgaat
     6901 actgccctta ccagggaaca ccctcggttt gggcaatcat ggactggatc gtggctgagg
     6961 ggttgcacaa ccaaggcctc gacccgctgt ttcatgatct ggcgcttctg cgcatgaccg
     7021 ccggtctgga tcgaagcggc accgccatcg agtcctggcc ggttcaccgc gaaacgggag
     7081 ccgtctgcta ccgtccgcac gagacaacaa cccgcggtga cgccgacctg gtgatggcct
     7141 gctctgaagt cggtcagctc cgccaggcat ggagtatcag ttccggcctg gcagcacttg
     7201 aggcacaggc aatcggctat cgggacgtac cgggaaacag ctggcaggaa caactctgca
     7261 aagaggcgct atcagccgta tctcatatct tcccggccgc agacaaccgt ccggaaattc
     7321 tcatccacat cgacctcgaa cgaggaaggg aactcaaggc gaaacgagtc gcccaaatgg
     7381 gtctggcggc ccattaaacc gccactgccc cggaggaccg caagaaacgc ccgtaaagcc
     7441 ggccgctctt gtggtcggcg gggcgccttg ctaactaggt agtggtttat tcgttaacgg
     7501 tcatttcggg tttacccact ttggcgctca agataagttg gcgggtttcg ccctcgtgat
     7561 ccttaatggt agtatcctgc gtctcgttat aataggtcac caaatggtga ctcataattt
     7621 cctgtaaatc tttctgtttg aattttagct cttccagctc actgttgatg gtttgataat
     7681 cccggtcggc ttccaacagt tttttagcct gttgacgctt agtcttaagt tcctttaact
     7741 cttcctgcag ctctttgtat tcctgattct gctcaaggac gtccttaagg ttgtcgcgca
     7801 tggccttgat tttagccctg gtttcttcca tttcagtcat taggcgatga atagccgcaa
     7861 ccgtcgtttc atcttccatg tgtggttttc gcctccttta gctttctgct cagtataggg
     7921 gtgatgaagg cgcgaaacaa ggcttgctgc ctcttgggcg caatgagatt tgcataattt
     7981 gccggcgcgc acaaattggg cacaattatg gcatggattg tacacaaatt aggcacacat
     8041 ctgtgccatt attggcacag cgtcccggac aaaaataaca ctctggcgct cggccaacct
     8101 acgcaagcgt tagtcgccaa taataattaa ctggcgctcg gcccgggtaa ctgccgtata
     8161 tagccaacga cgccagtcgt catcattcat atgtttattg cgttcttcaa atagcaggac
     8221 ggtgtcggct gaagacccct gggctttatg aacggtcagg gcgtagccaa aatcaaacaa
     8281 atcaccgcgg acttttggct cgggcgaagg cggcacttta gcaatggtct cccgcgaacc
     8341 aaactgttcc cgcatgagat agcaatggcg ctcggtgtca tcaatcataa tgccagcctc
     8401 ccaccaggtg gcttcaacat caccggacac cgattgaatc cacttcaggg ttccggtcat
     8461 gccgttatac agcttgccgg cggtattgtt gcgtaagcaa atcaactgct cgccaggctc
     8521 cgggttcgga gtttcaaaac cgcgcctggc ccgcagctca cgattcagcc ttactcttgt
     8581 atggttatag ccgcatagta ccagccattc ggggctccaa gcttccagca gctcatccat
     8641 agccgcaccc agctcgttgc tatgccggtc atatttaacc acctcgtctc cataacgccc
     8701 cggtgcaatt gtgccggtcg tacgagccaa tgtagcaacc tccacaatcg ggctatcaat
     8761 agcctggcga tagacctttt caagccggat catcggctca ttcatgaggt tgaaattggt
     8821 gccaatcggc ggcagctggc cgtgatcgcc gacagccaaa atcggcacct tatagctcag
     8881 taaatctttc catatatcct cggttaccat actggcctca tcgaccataa ttaaatcagc
     8941 ttttatagtt tggcgacggc gccatccagt aacttggccg tccgaaccca actcggtcgt
     9001 gtatatcagc ccgtgtatgg ttgtcagact atcgccctta cggctgactc cttggcgctt
     9061 cagtgtttga tccaataccc gcactgcttt accggtaaaa gcgcagaagg ctactttaac
     9121 atcggggtcg ttatcgcgca gcgcctgacg gacatgtccg agcatggttg tcttacctgt
     9181 gccggcatag ccgcccaagg tcacatagcg ctgggtaccc gatcgatacc accggccaat
     9241 ggtttgcagg gcttcaatct ggtcgggtga taattgatac gacattcagg tttaagttta
     9301 ccacccgcga ttccaaaaaa ttatgtgctg ggagccactc cgcttgtggt aactaccaat
     9361 gcgggtgacg cctggccggg cgtagagtgg cattatagcg taagaggagg gtctgctatg
     9421 acagcagtaa aaagggcaat gtccgacgta caagctacga caatcttagg gtcagaagca
     9481 gcccctatag ataaggttcg gcagctggtt gcgatcggct atgaagagga ggttgccgac
     9541 ctcatggtct cgggtgttca atccgggcaa catcaaatgc tttattatga gcagttgcca
     9601 caagctgatt acgccgacga ttaggccata gtaagtctta cgtacggtat acttaaggga
     9661 tatgaagtac ggcttgcaca tctccacagc gggaaattta tcgacaacgc ccggccgggc
     9721 cgaggcgatg accgctgctg ccttgcaaat ttttgccggc agcccgcgta catttaagca
     9781 gccaacctat acgcccgaga ttactgacgg tttcaaacaa gcctgcaccg atcttaagct
     9841 gccttcattc attcatatgc tctacctaac cagttatggc tcgccggacg ataaactccg
     9901 ggattcctcc attgcggctg ctaagcaaac catgcgcaat gccgacctgc ttggggtgga
     9961 gggcgttgta actcatctcg gctcgcacaa aggtgaggga acggctgcgg ttatagaccg
    10021 tttgcgtgat gcgctgcttg atgttgtgga cggggttgaa accaagttgc tcttagaaaa
    10081 ttcggccgga gccggtggga acgtgggcaa cagcattgaa gagcttgccc tcatttatga
    10141 agccgtcggg cgcacgccaa aaattggctt ttgccttgat acggcgcatt tatttgcatc
    10201 agggtatgat gttcgtaccg atgaaggctg gaagggtgtg ctggatgagt tcgacgctaa
    10261 aatcggctta aaaaatcttg gcgtcattca cctaaatgat tcaaaagttg atttaggcag
    10321 taagcgagac cgccacgaaa acatcggcaa cggttttatt ggcgaagccg gcttcaaggt
    10381 tattctgaac gaaccccgcc tggatggcat ggtagggtta ctggaagtgc cgggaattga
    10441 cgacaaaggc cccgataagc ccaatttgga taaattgcga gagcttaccc ttagcgcctt
    10501 atgatccctg tgttcgagcg gcaaaccgat actccgctgt ttccggacat gctctggaac
    10561 cgtccagcca tgaaaagccg cggcgggcgc ttgctggtac tcggcggcca aacaaatgaa
    10621 tttagcctac tgcaagcaat ttatcagatg gccgaaaccg ctggaatcgg agaggtagta
    10681 gtggccatgc cggacagctt gcgccgtagc gcaggcaagg ctggatatgg acagtttgta
    10741 ccggcgtcgt cgtccggatc gctgggaaaa gctgctttag gtgaaattgt gcatctggcg
    10801 ggggaagccg atgcggttat tattggcgcc aatcttacca acaatgccga gaccggcatg
    10861 atgattgagt cactcgtccg cgagcttgat tcgcgggtta tctttaccga agaagcattt
    10921 gaaatcctgc agtttcatcc tgacttcatt accggcaatc cgaaggcact ggtagttgcc
    10981 actatgaaag gtctgttcga cctggccaac caccataaaa tgccaattgc gattcggccg
    11041 aatgccggag tcatcggaaa gctagaaatt ctggaacaga tagcatcaat cagcaaatgc
    11101 cagtatgtag tgtttgataa ggaaattatg gttgccgccg aaggagagct gagcctgacg
    11161 ccgcttaata ccgctcttag cagcctgcct gccgccccaa ttggcatagc ggccaccttt
    11221 tgggtccagc agccgggcaa gccgtacgag gccctaacaa cggctggcta tgttttagcg
    11281 gcagccttgc aaaaagacac aactactcca agctatagca accttagcaa gcaagttgaa
    11341 gcggtacttc gagagcaaga tcaataatgt tttgctggcc aagatcacta ataaaatcag
    11401 tcgataattc gtcttagtaa aatagtatcc gtaagatact acgcagcaag taaaaaatag
    11461 tatcctatgg atactaaacg tataaaacta atgtcacttg ttcagcaatc gcttaagcca
    11521 tcatggtgca taaaatgaat tcgggcggtt tccaccgcgt ttatttaaaa agttgtaatt
    11581 attcctggta ccgctggtcg ggctcgaacc gacacgctat tgctagcacg ggattttgag
    11641 tcccgcgtgt ctaccaattc caccacagcg gcatatcagt tgcggttggc tatcgaacct
    11701 aaaaagatta tcatacttac atactgaatt gaattgccac gacctatgag ccaatctgaa
    11761 attgtccgtt ttccccgatc atccatgccc aaccgcgata acaacccaga cgcggctaaa
    11821 gaggcgcgcg agcggattgc cggtataaat agtccgtatt tagcccatgc cgcacccgcc
    11881 aaggaagatt tggtgggggc aattcatcct atcagcccgg cggccaacgt ccaagacggc
    11941 aagcccaccg ctgatgctcc gccgtttacg attagctcct ccgatgatga gcctcagcca
    12001 gccccagcag ccgaaacgac ggtcagcgcc caaccagcca ctcccgaggc tcatgatcac
    12061 ttgctgcctc cggcccatgg cgcaaacgcg ctgccaaccc aaccgcaagc ggcgcaaaca
    12121 caaccaacgc caaccatacc gccgccaaat cccctagagc agctatcctc aggtgtaccc
    12181 aagaagaaaa ctagccattc caaccgcccg cgaacggtag ctcagcccaa gagcctaccg
    12241 agccgtttta tgccgctgat tgtgggtgtg atcaccttcg tcctggtact tttggttgta
    12301 aaagcgccca ttctgctcaa tcagcttagc tacttcacca acgacaagcc gccggccgct
    12361 caaaatgccc ccgccgcaaa tgtggtggcg gtttcaccgg acccggttat tacgattccg
    12421 aagattaacg ttagcgcacc ggttatttat accgataata acgctgaatc aatcattcaa
    12481 aaacatctgg aaagcggggt cgttcattac gccgggaccg cactgccggg aaccaatggc
    12541 aatagcgtaa tattcggcca ttcgtcgaac gactggtggg agccgggaaa ttataaattt
    12601 gtgtttgtgc tgctcgataa acttcaaccc aatgacacct ttacggtgaa ctacaactcc
    12661 cgccaatacc tctatcaagt gaccggcagt aaggtagtgc agcccaccga tttgagcgta
    12721 ctcaacccca ctccggagcc aaccatgacc ttaattacct gtacgccgcc aggtactagc
    12781 tggcagcgct tagtggtaac cgctaagcag attagcccgg ttgctgaaac tgaaaaagct
    12841 ccagctatcg atgaagaggt tgcagccgac gggacactgc cgggcaatgc tcccggcatt
    12901 ggccagcgcc tctccgattg gtggaaaggc ctaaccaacg tttttactcg cgaataataa
    12961 cgcgcctaat atgaatggcg ccggcagcgt tcggcgaggc tacaattacc gaacgggtat
    13021 cgcccgacac aaacgccggc aacaccccgc ctttggtgag agtgttcagg taattgccat
    13081 cgtattcgct cagcataatt gatccgctct ggttagatag caagtggaag ttgcccagcc
    13141 aagctactcc gctggcagcg ttcaccgatg gcggcaaact ataggtcgta ttttcctgta
    13201 gatcataggt ggcgctatgc gattcatcga actgcagtaa aaagcgaccg tcgccattaa
    13261 atacggcgct tgctgcaaca gccggcagtt caatcggacg cggactgtga tacagattcc
    13321 gatacagcag ggtcttgcgg ctatctaccg ccaccaccgc cagcatcggc gtacgaatgt
    13381 aatgagcaaa gtcgagatta taggtttggc tgacgggcaa attgtcggcc aacctcttgg
    13441 tttcgccgtt ggcatcaagc acccacagcg acggtgtggg ctcagcatgg ctgacgtaaa
    13501 gcatgcgatt gccgtcaaag gtaaatccgg caacgttcga tgccagaacc gctgatatgc
    13561 tgttattgcc catatccaag cggcgcaatc cttgatcgct catccaatag agctggcgcc
    13621 agttggaagg actgaaggta aggtcaggcg g
//
LOCUS       DASVGB010000018         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1259225, whole genome shotgun sequence.
ACCESSION   DASVGB010000018 DASVGB010000000
VERSION     DASVGB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259225"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VF272_00545"
     CDS             complement(<1..986)
                     /locus_tag="VF272_00545"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7259404.1"
                     /translation="MINFDVRVETLKGVGTAMAIKLERLGIATIGDLLTHYPRRYDDF
                     SEIIPIRSMKPGLVTFKGTVERIASRRVRGRKLQLTEAIITDGTGTVKAVWFNQSFLA
                     AQHPVGTEVFVAGKVEFRNNDLALQSPAIEKADSQGIHTGRIVPVYPETDGLTSRQLR
                     NLIAPLLPRLDDMAETLPASVLTEAKLLGRSQTLIEIHAPTTQTNLQRARHRLAFEEL
                     FYIIGASLIIKNEIKTESADPISLKVEVAQAFTKSLDFDLTNAQRAAAWQILQDMQDI
                     TPMNRLLEGDVGSGKTVVALLAASMVMASGHQVALMVPTDILARQHAATIEP"
     gene            complement(988..2151)
                     /gene="tyrS"
                     /locus_tag="VF272_00550"
     CDS             complement(988..2151)
                     /gene="tyrS"
                     /locus_tag="VF272_00550"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7259405.1"
                     /translation="MIDLVLRRAKDVVVQADLERLLSTKKLRIKHGIDPTGPRLHLGR
                     ASTLHRLRNLQDMGHTIVLIVGDFTAKIGDASDKASERQPLSDTEIKANMTDYERQLA
                     LVLDMNQVELHYNSAWLSKLTSNELIELAQQFTVAQMIERENYAIRFKENRPIGLHEF
                     LYPILQGYDSVAVEADVEVGGTDQLFNLMAGRTLQKHYNQPPQQVMTFDLLTGPDGRK
                     MSTSWGNAIYITAEPNDLYGQIMRINDELIAQYYRVCTDIEENVIQTAVLDIAAGANP
                     RDTKASLAREIVRLYHGDDVALAAEDAFNKQFRDKQLPDDIKEQHVTKATWEPIELLV
                     ALKLADTNSAARRLIEQGGVRINDEKVDGTSMQVKSGDIIQVGKRRFVRLTVR"
BASE COUNT          555 a          587 c          515 g          582 t
ORIGIN      
        1 aaaggctcaa tggtggctgc atgctggcgg gcaaggatat cggttggaac catcagtgct
       61 acctgatggc cgctggccat caccattgac gccgcaagca atgcgaccac cgttttcccg
      121 ctgccgacat caccttcaag taaacgattc ataggagtaa tatcctgcat gtcctgaagg
      181 atttgccagg ctgcagcccg ctgagcgttg gtgagatcaa aatctaggga cttggtaaag
      241 gcctgggcta cttcgacttt aaggctgata gggtcagctg attcggtttt tatttcattt
      301 ttgataatca aactggcccc gataatataa aaaagctctt caaaggccaa tcgatggcgg
      361 gcccgctgca aattggtctg ggtggtagga gcgtgaatct cgatcaatgt ttggctacgt
      421 ccaagcaact tggcttcagt gagcacgctt gcgggcaaag tctccgccat gtcatcaagc
      481 cggggcaata aaggcgcaat caaattacgt agctggcgcg aggttagccc gtcggtttcc
      541 gggtaaactg gcacgatcct gccggtatgt atgccctggc tatcagcttt ttcaatggca
      601 gggctttgca gtgctaaatc attattgcgg aattcaacct tgccggctac aaatacctct
      661 gttcctacag gatgctgcgc tgccaggaaa gactgattaa accacaccgc cttaacagta
      721 cccgtaccat ctgtaattat cgcttctgtc agctgaagtt tacggccgcg taccctccgg
      781 ctagcaatcc gctcaaccgt ccctttaaag gttactaatc ccggcttcat cgatcgaatg
      841 ggtatgatct ccgaaaaatc atcataccgg cgcggatagt gtgtaagcaa atccccaatg
      901 gtagcgattc cgaggcgctc aagcttgatc gccattgccg taccaacacc cttaagggtt
      961 tcaacccgaa catcgaaatt tatcatgtta ccgtaccgtt agccgcacaa atcggcgttt
     1021 gcccacctga ataatatcgc cgcttttcac ctgcattgat gtgccgtcaa ctttttcatc
     1081 attaatgcgc acgccgcctt gctcgatgag gcgccgggcc gcgctgttag tgtcggctaa
     1141 tttcagggca accagcagct caataggctc ccacgtcgct tttgttacat gctgctcttt
     1201 aatgtcatcc ggcaactgct tatcgcgaaa ctgcttatta aatgcgtcct cagctgccaa
     1261 ggctacgtca tcaccatgat acaagcgtac tatttctcta gccaatgagg ctttcgtgtc
     1321 acgcggatta gcgccggcgg caatgtctaa cactgccgtc tggatcacat tctcttcaat
     1381 atcggtgcag actcggtaat attgggcaat caactcgtca ttaatacgca ttatctgtcc
     1441 atataaatca tttggttcgg cggtgatata aatggcatta ccccacgaag tcgacatttt
     1501 acgtccatca ggaccggtta acaaatcaaa tgtcatgacc tgctgaggcg gctggttgta
     1561 gtgcttttga agtgtccggc cagccataag attaaaaagc tggtcggtgc cgccaacctc
     1621 gacatcagct tctaccgcca ccgaatcata tccttgcaaa atcggataca gaaactcatg
     1681 caggccaatc ggtcggtttt ccttaaagcg aatcgcataa ttttcccgct caatcatttg
     1741 ggctaccgta aactgttggg ccaactcaat cagctcatta gacgtaagct tactgagcca
     1801 cgccgagtta tagtgaagct cgacctggtt catatcaagc accagggcta actgacgttc
     1861 atagtctgtc atgttggctt taatttcagt atcgcttaaa ggttggcgtt cactggcttt
     1921 atctgatgca tcgccaattt tagctgtaaa gtccccaact ataaggacaa tggtatgacc
     1981 catgtcctgc aaattgcgca accgatgcaa ggttgaagcc cggcctaaat gcaggcgcgg
     2041 accggtcgga tcaatgccgt gcttaattcg cagcttttta gtgctaagca gccgctctaa
     2101 gtcggcctgt actaccacgt ctttagctcg tctcaatacc aggtctatca tcgttcgtat
     2161 catagcacag acgaccgaac atatcagaag aagcacaagc aaaatgcttg gacaaatacc
     2221 cgtgccattt tataatgga
//
LOCUS       DASVGB010000019         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_841220, whole genome shotgun sequence.
ACCESSION   DASVGB010000019 DASVGB010000000
VERSION     DASVGB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841220"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF272_00555"
     CDS             <1..251
                     /locus_tag="VF272_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259406.1"
                     /translation="SYNVAATSVATDRTRKATVIIDHTGGAKPYTIRYLKQRFNATVQ
                     TQTKSGSLTAEPDIAIVVGSDYNLAASSPVTPASTTKP"
     gene            248..1009
                     /locus_tag="VF272_00560"
     CDS             248..1009
                     /locus_tag="VF272_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259407.1"
                     /translation="MKPQTFFMTLLYLGLAGAVLVLGHYGFALVAAVLVTVPVLIQWI
                     RSGRSRNALVGAIPPVMVGLSVVILVGLSKPPIGQPVFPVSTQVVLAALYGTWLIWLT
                     QIRNAGQANWLIAGILQVTTTMAIFLASAFWHWPSAMVIAVVWGASFVIAWWYLSEVG
                     ERAAAILAATWALVTAELSWILAAWQVNYILYDGLMIVPQATIIILGVGYCFASIYHS
                     HSTKRLSRRRLIEYSVIAGVLLAIIIAGTRWNGTL"
     gene            1048..2205
                     /locus_tag="VF272_00565"
     CDS             1048..2205
                     /locus_tag="VF272_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7259408.1"
                     /translation="MANSSPDPDYNSDSEDRNLPYGVLRRLITLTLAISVVSGIIGAF
                     VIVNYFPGSIPVTKRAVILQENSATIEVAKKVSPSVVSITSESIGFSFFGGAQTQKGA
                     GTGFLVSEDGLIVTNNHVIEGATTVTVFDSTGKQYKNAEVVATDPQNDIAFIRIKATG
                     LKPLTLGDSGRVQVGSRVVAIGNALGQFQNTVTEGIISGLGRPVVAGDELGNNLEQLQ
                     NLFQTDAAINPGNSGGPLVDLSGNVIGINTAVAGGAQNIGFAIPINEIKKALATVKSR
                     GSIIKPFLGIRYIPVTPAFAAQNNLPVNAGVYVAGDEQNPAVVPNSPAAKAGLKDGDI
                     ITKIGNQAIDEKHSLSSLIAEHAPGEKVPVTFLRDGKTQTAEVTLEEAPKQ"
     gene            complement(2211..3020)
                     /gene="prmC"
                     /locus_tag="VF272_00570"
     CDS             complement(2211..3020)
                     /gene="prmC"
                     /locus_tag="VF272_00570"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7259409.1"
                     /translation="MTIAEAVKLGVGMLRAAGIESARIDTYLLLSKVLAKDRAWLMAH
                     PDHEINEDQAQSYMDLTNQRRQRVPLVHLTNKREFYGLNFYIDERVLTPRVETEKMVE
                     YAIAYAPQHSRLLDVGTGSGAIAIAIAKHRPDLEIWATDISKDALTVAQQNADSHKVL
                     LNLAVSSLLDQVSGRFATIVTNLPYLRDDADLMPEVRKEPGVALFGGSDGLELYRDFL
                     KQLEGYLEPKAYVFTECDPWQHEDMTKEARKAGLTPSKTEDYFILGFQQQG"
     gene            complement(3022..4047)
                     /gene="prfA"
                     /locus_tag="VF272_00575"
     CDS             complement(3022..4047)
                     /gene="prfA"
                     /locus_tag="VF272_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7259410.1"
                     /translation="MRTEYAEVQRKLTDSSLFGTPEYVPYAKRQTEMQPLIELYDLRK
                     LLEDDLEALNDPELADMAATERPAIEARLKEVENKLRLALVPKDPNDAKDVIVEIRAG
                     AGGDESSLFAAELYRAYARFAEAKGWKLELDSESPSDVGGFKEVMFEIHGTNVYAEMK
                     YESGVHRVQRVPATESQGRVHTSTITVAVLPEAEETELVINDADLRIDVYHASGHGGQ
                     SVNTTDSAVRITHLPTGVVVAMQDEKSQLKNKQKAMSVLRSRLLAAKLEQEQKENREA
                     RLAQIGTGDRSEKIRTYNFPQDRITDHRIGRTQHGLPGYMAGEIGPMIEALQAEDLKQ
                     RLAEAGA"
BASE COUNT          928 a         1017 c         1042 g         1068 t
ORIGIN      
        1 atagctataa cgtggccgca accagcgtgg ctaccgatcg tactcggaag gcgaccgtca
       61 tcattgatca taccggcggg gccaagccgt atactatccg ttatctaaag cagcgtttta
      121 atgccacggt gcaaactcaa accaaatccg ggagcttaac ggctgaacct gatattgcta
      181 tagtagtggg aagcgactac aatcttgctg ctagtagccc cgtaactccc gcatcaacaa
      241 ccaagccatg aaaccacaaa cattttttat gaccttacta taccttggat tggccggggc
      301 ggttttagta ttgggccatt acggctttgc gttggttgcg gccgtgcttg ttaccgttcc
      361 ggtacttatt caatggatcc gttcggggcg cagcaggaat gcccttgtag gggccatccc
      421 tccagtaatg gtgggtttaa gcgtggttat tttggtcggc ttaagcaagc ccccaattgg
      481 tcagccggta tttccggtaa gtacgcaggt tgtgctggcg gccctttatg gaacctggct
      541 catttggctg actcaaatcc gaaacgctgg ccaggccaac tggctgattg ccggaattct
      601 tcaagtaacc accacaatgg ctattttctt agcgtcggcc ttttggcact ggccaagtgc
      661 catggtcata gccgttgttt ggggcgctag ttttgtcatt gcttggtggt atttaagtga
      721 agttggcgaa agggctgctg ccattttggc tgctacctgg gcgcttgtca ccgccgagct
      781 aagctggata ttggcagcat ggcaggtaaa ttatattttg tatgacgggc tcatgattgt
      841 accgcaggct acaattatta ttttgggggt cgggtactgt tttgccagta tttaccattc
      901 gcacagcact aaacggttaa gccgccgtcg cttgatcgag tatagcgtga ttgcgggagt
      961 tttattggcg atcattatag ctggaacacg ttggaacggc acactctagg ctggagttac
     1021 gaatagtgca ggtgtataaa ggatagtatg gcaaattctt cacccgatcc tgattataat
     1081 tccgactccg aagatcgcaa cctgccgtac ggtgtgctcc gccgtttaat aacgcttaca
     1141 ttggccatta gcgtagtaag cggtattatt ggcgcctttg taattgtaaa ttactttccc
     1201 ggcagtattc cggtaaccaa gcgagcggtc attcttcagg aaaattcagc aaccattgag
     1261 gttgctaaaa aagtttctcc cagcgtagtg tctattacgt ccgaatcaat cggttttagt
     1321 ttctttggcg gtgcgcaaac ccaaaaaggg gctggcactg gctttctggt ttctgaggac
     1381 ggcttgatcg taaccaacaa tcacgtgatt gaaggagcaa caacggtaac cgtgtttgat
     1441 agtaccggca aacagtataa aaatgccgaa gtggtagcta ccgatccgca aaatgacata
     1501 gcttttattc gcattaaagc cactggtctc aagccgctta cattgggcga ttccggcagg
     1561 gttcaagttg gcagccgtgt ggttgcgata ggaaatgccc tagggcagtt tcaaaacacg
     1621 gttactgaag ggattatttc cggtttggga cggccggtgg ttgccggtga tgagctgggg
     1681 aataaccttg agcagctgca gaatctgttc caaaccgatg cggcgattaa tcccggtaac
     1741 tccggcggcc cgctcgtaga tttaagcggc aacgttattg gaatcaatac cgcggttgct
     1801 ggcggggctc aaaatattgg ctttgcaatt ccgattaatg agattaaaaa agctttagca
     1861 acggtaaaat cccgaggcag tattatcaag ccgtttctcg gaattcgtta cattccggtt
     1921 actccggcat ttgccgctca aaacaattta ccggttaacg ccggcgtcta tgtagctggt
     1981 gacgaacaga atccagcagt cgtgccaaac agtccggcag cgaaggccgg gcttaaagac
     2041 ggtgacatta ttaccaaaat cggtaatcag gcaattgatg aaaaacatag cctttcttca
     2101 ttgatagccg agcatgcccc gggcgaaaaa gttcccgtca cctttttacg cgacggtaag
     2161 acgcaaacgg ctgaagtaac gctggaagaa gctcctaagc agtagttttg ctagccttgc
     2221 tgctggaagc ctaagataaa atagtcttcg gtcttacttg gtgtaagtcc ggccttcctg
     2281 gcttcctttg tcatatcctc gtgttgccac gggtcgcatt cggtgaaaac atacgcttta
     2341 ggttcgaggt agccttccag ctgcttaagg aaatcgcggt acagctccag tccgtcgctg
     2401 ccaccgaata aagccacgcc gggctccttg cggacctcgg gcataaggtc agcatcatca
     2461 cggaggtagg ggaggttcgt aacaattgta gcgaagcggc cggaaacttg atccaataaa
     2521 ctggagactg ccagatttag caaaacctta tgggaatcgg cattctgttg agctaccgtc
     2581 agagcatcct tgctaatatc ggtcgcccat atctccaggt cggggcgatg cttggcgatg
     2641 gcaattgcaa tggcgccgct gccggttccg acatcaagta agcgtgaatg ttgtggagca
     2701 taggcaatgg catattcaac cattttctcg gtttcaaccc gtggcgttaa cacccgttca
     2761 tcaatgtaaa agtttaatcc ataaaattcc cgcttattcg ttaaatgaac caaggggact
     2821 cgctgccggc gctgattagt aaggtccata tagctttgag cctggtcctc gttaatttca
     2881 tgatcgggat gcgccatcaa ccatgctcga tccttggcaa gcacctttga aagcaacaga
     2941 taggtatcaa tacgggcgct ttcaatgcct gccgcacgca gcataccgac tccaagctta
     3001 acggcctcgg caatggtcat attaggctcc ggcttcggcc aaccgctgct tcaaatcttc
     3061 agcctgcagc gcctctatca tcgggccgat ctctccggcc atgtagccgg gcagcccatg
     3121 ttgggtgcgt ccaattcggt gatcggtaat acgatcctga gggaagttat aggttcgaat
     3181 tttttccgac cggtcgccgg taccaatttg ggccaaccgg gcttcgcgat tttctttttg
     3241 ctcttgttct agtttggcag ctaataagcg cgagcgcagc acgctcatgg ctttttgctt
     3301 gttcttgagc tggctctttt catcctgcat cgccacgacc acgccggtgg gcaggtgagt
     3361 aatccgcacc gccgagtcag ttgtgttaac gctctgcccg ccatgcccgc tggcatgata
     3421 tacgtcgatc cgcagatccg catcattaat taccagttcg gtttcttcgg cttcaggcag
     3481 caccgctacc gttatggttg atgtgtgaac ccgaccctgg ctttcggtgg caggcacccg
     3541 ctgcacgcgg tgaacaccgg attcgtactt catttcggca tagacgttag tgccgtgaat
     3601 ctcaaacata acttccttaa atccgccgac atcgctaggg ctttccgagt caagctctaa
     3661 cttccagccc ttggcttcag caaagcgggc ataggctcgg tacagttcgg cggcaaacaa
     3721 ggatgattcg tcaccgcccg caccggcgcg tatttcaaca attacgtcct tagcatcatt
     3781 cgggtctttt ggtaccagtg ctaagcgcag cttgttttct acctctttca acctggcctc
     3841 aatcgccgga cgttcggtgg ctgccatatc ggctagttcg ggatcattaa gtgcttccag
     3901 gtcgtcctcc agcagctttc gcaaatcata cagttcaatg agcggctgca tttcagtctg
     3961 acgcttggca tagggaacat attcgggcgt gccaaacaag ctgctatcgg ttagcttacg
     4021 ctgaacctcg gcatactcgg tacgcagttt gtgtt
//
LOCUS       DASVGB010000020         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1257978, whole genome shotgun sequence.
ACCESSION   DASVGB010000020 DASVGB010000000
VERSION     DASVGB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257978"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1776)
                     /locus_tag="VF272_00580"
     CDS             complement(235..1776)
                     /locus_tag="VF272_00580"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7259411.1"
                     /translation="MAAPVIAKPTTSPETIKIGQRITELREAKQLTQSALAKLLGTSQ
                     SAVARMEKGDQNISTKMLVKMGEVLDAEIIALSSYGLNFKIEGGRKLSGTVTTNTSKN
                     SAVVMLCAALLNQNTTTLKRMPRIEEVNRVIEVLESLGVQIKWQGNDVVITPPKRLKM
                     ESINVKAAVRTRSVLLLLGPLVHLLDEFSLPQAGGCHLGARTVRPHLYALERFGVAIE
                     AKTNSYAVSAGQLEAAEVVLYESGDTVTENALMVAARLPGLTTIKFASANYMVQDLCV
                     FLQTLGVRIEGIGTSTLRVWGKPSINKPVTYGISEDPIESMLFLTAAIMTKSQITIKR
                     CPIEFLELELLKLEKMGFQYKRSAKYPAANGYTWLVDLETFPSKLTALEEKIHPSIYP
                     GLNMDNLPFFVPIATQAKGQTLIHDWPYENRAIYYMELTKLSAELILGDPHRVFVNGS
                     TKLRAAEVICPPALRPAAIILIAMLAAQGTSILRNVYSINRGYEDLYARLNSLGADIQ
                     VLREL"
     gene            complement(1857..>2993)
                     /locus_tag="VF272_00585"
     CDS             complement(1857..>2993)
                     /locus_tag="VF272_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7259412.1"
                     /translation="EYHSATGQYYLHNFFKAQPDLNWRNPEVLKEFDSIMRFWLDKGV
                     AGFRLDVFNMIIKDISFRDNPKSIKTDEPEIRLYRQRPIYNTSQPEVHAILKRWRLTT
                     DEYSSDRLLLGESNRVYDMKVLAGFYGKQDELQLVFNLAFVDAPFKATQLRDLVEKTE
                     AAFMQNQWPAWTGSNHDKPRMASRWAAGDEHKHRIALMMLLGLHGTPVLYYGDELGME
                     DVYIAPWRLKDPQGRKYWPVNGGRDRARTPMPWKNQHGAGFTDENVRPWLPYGDLRVR
                     SVDSQMHIKDSTLQFSKDLIALRQSEPDLYAGSYESLPTVDSVWAWKRGKNTVVAMNF
                     SNYHHEIKNISGKIVLGTQRKRQGETVKGLLHLEPWEGAIVKGR"
BASE COUNT          747 a          784 c          684 g          778 t
ORIGIN      
        1 cgctgccata tcaggcttag taaagagcgt tgtatccaaa actgaagcga ttcctacgat
       61 aaccgcatat acagctgccg gcttcatatt aagcttgaac ccatcccatg cccggttaat
      121 gataccgtca atccccggcc agctgtcatt ggcgggtgcg gcgctcggag tattcatcgc
      181 aataattcct agttattgcc ttagtatgac aagagtagaa agcaaacgtc aagcttaaag
      241 ctcgcgcaga acctgaatgt ctgcaccgag actgttgagc ctggcataca aatcctcata
      301 cccgcgattt atcgaataca cattacgcaa aatcgatgtc ccttgtgccg ccagcatggc
      361 aattaaaata atggcggctg gacgcaaggc cggtggacaa atgacttctg cagctcgcaa
      421 ttttgtcgag ccattcacga atacccggtg cggatcgcca agaataagct cggcgcttag
      481 cttagttagc tccatataat agatagcgcg gttttcgtat ggccaatcgt gaatcagtgt
      541 ttgacccttg gcctgggtag cgatgggcac aaagaatggc aggttatcca tattcaatcc
      601 cggataaatg ctaggatgaa ttttttcctc cagagcggtc agcttagagg gaaatgtctc
      661 aagatcaacc agccacgtat agccgttggc tgccggatac ttggcgctgc gcttatactg
      721 gaagcccatt ttttccaact tgagcagctc cagttctaga aactctatcg ggcagcgctt
      781 aatcgtaatc tgcgatttag tcatgatggc cgctgttaaa aacagcattg attcaatcgg
      841 atcttcgctg atgccgtagg tgaccggttt attaattgat ggcttgcccc agacccgcaa
      901 ggtgcttgtt ccaatgcctt caatacgaac gcccaaggtc tgcaggaaga cgcagagatc
      961 ctgcaccata taattcgccg aagcaaattt aatggtagta agaccgggca agcgggccgc
     1021 caccattaag gcgttttcgg taacggtatc ccccgattca tacagcacga cctcggccgc
     1081 ttcaagctgt ccggcactca cggcataact attggtttta gcttcaatcg ccactccaaa
     1141 ccgctcaagt gcatagagat gaggtcgtac cgtccgggct cccaggtggc atccgccggc
     1201 ttgaggaaga ctaaactcat cgagcaaatg caccagcggg ccaagcaaca gtaaaacact
     1261 ccgggtgcga acagctgctt tgacattgat gctttccatt ttaagccgct tcggcggagt
     1321 aataactacg tcgttaccct gccatttaat ctggacgcca aggctttcta aaacctcaat
     1381 gacccggtta acctcctcaa tccgcggcat tcgtttcaat gtcgtagtat tttgattcag
     1441 cagtgccgcg catagcatca ctaccgctga attcttagag gtattggtgg tcacggttcc
     1501 tgacaatttg cggccgcctt caattttgaa attcaagcca tagctagaca gggcaataat
     1561 ttcggcgtca agaacttcac ccattttaac tagcattttg gtactaatat tttgatcgcc
     1621 cttttccatc cgcgcgaccg cactttggct ggtgcctaat aatttagcca aggccgactg
     1681 ggtaagctgt ttggcttcac gcagctcagt gatacgctgg ccaattttta tggtttctgg
     1741 tgaagttgtg ggtttagcga taaccggagc agccataaca cgcccttcat attaacgtca
     1801 ttcatatcat atatgagatt caatatgctt gtaaacccaa ctttccaaag atttagctaa
     1861 cgacccttaa caattgcccc ttcccatggc tctagatgca gaagcccttt taccgtctca
     1921 ccctgccgct ttcgctgcgt accaagtaca atcttaccgc taatgttttt tatctcatgg
     1981 tggtagttag aaaagttcat tgccactacg gtattcttac cgcgcttcca cgcccatacg
     2041 gagtcaacgg ttggcaggct ttcataggaa cccgcataca agtccggttc actctgacgc
     2101 aacgcaatca agtctttgct aaactgcagg gttgaatctt ttatatgcat ctgagaatca
     2161 acgctgcgga cacgaagatc gccatatggc aaccaaggcc taacattttc atcggtgaat
     2221 ccggctccat gctggttttt ccacggcatg ggtgtccgtg cccgatctct ccccccatta
     2281 accggccagt acttgcgccc ctgcggatcc ttgagccgcc agggagcgat gtagacatct
     2341 tccatgccca attcatcacc atagtacagc actggtgtgc catgtagtcc taaaagcatc
     2401 attagggcaa tccggtgctt gtgctcatcg ccagccgccc agcgcgatgc catacgcggt
     2461 ttgtcgtgat tggatccggt ccaggcgggc cactgattct gcatgaaagc cgcctcggtt
     2521 ttttcaacca gatcgcgtaa ctgcgtggcc ttaaaagggg catcaacaaa cgctaagttg
     2581 aatactaatt gcagctcatc ctgcttgccg taaaagccgg ccagcacctt catgtcgtaa
     2641 acccgattgc tttcacctaa cagcaaacga tcgcttgagt actcatcggt ggtcagccgc
     2701 caacgcttta aaatggcatg aacctcaggt tgtgaagtat tgtaaatcgg ccgctggcgg
     2761 tacaggcgaa tttctggctc atcggtctta atgctctttg gattatctcg aaaacttatg
     2821 tctttaataa tcatattaaa gacatccaag cggaagcccg ctacgccttt atccagccag
     2881 aagcgcataa tactatcaaa ctcttttaga acctcgggat tgcgccagtt aaggtcgggt
     2941 tgagctttaa aaaagttatg aaggtagtat tggccggtag cggagtggta ttc
//
LOCUS       DASVGB010000021         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_831471, whole genome shotgun sequence.
ACCESSION   DASVGB010000021 DASVGB010000000
VERSION     DASVGB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831471"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VF272_00590"
     CDS             <1..1057
                     /locus_tag="VF272_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7259413.1"
                     /translation="ALRQPGSAFKPIVYARAIEDKTITAATVLKDRPRTFAANYRPQN
                     YDRKFRGDVLVRRALANSLNVPAVEVMEKVGTNNVLTMAGNMGITTLKSVSDYGPSLV
                     LGTGETRLTELTSAYGVFANGGKRIPITTITKVQNKQGKTIFRNRQKSTQVINPAVAF
                     IVSSILSDNAARAELFSNRLNVNRPAAVKTGTTEDYRDALTVGYTPSVVVGVWVGNND
                     NTPMDNIAGSLGPAPVWRSLIERYSKGLSSESFKPPGNVAALSVCRSNGLKALRPGAA
                     TYTEYFLERTEPTQSCNVPAPAPKPKNDKNDVIKPKEEERPGEEPLDDTPVEPTPSPT
                     PEPTPTPLPPPQPPVTP"
     gene            complement(1054..1773)
                     /locus_tag="VF272_00595"
     CDS             complement(1054..1773)
                     /locus_tag="VF272_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX7259414.1"
                     /translation="MQNILHEIPRPNKRFLQTIQNVEVHDHVCLLYRDSQEGLASAAA
                     FIEAGLRKHDQCIYISDDNTPEAVLQALEHAGITTENMLETEQLVLGTKDGSYLENGS
                     FSPDTTIKVFAEASHQATSKGFRAVRGCAEMTWQLADDSHTETEHLLEYEARINDELF
                     PKHQVVGMCQFNMNRFGAAAIREIIYTHPVVIIGGLMCENIYYKPPGMHRVNSGNDLA
                     ELEVKKMVNDVYRFAERRYGF"
     gene            2005..2202
                     /locus_tag="VF272_00600"
     CDS             2005..2202
                     /locus_tag="VF272_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259415.1"
                     /translation="MAKSTKVMNHGSLGGVFLATYIGAAVYFLQHTQGFWGTIWALIE
                     ALFWPAFIIYHVLTQLQVSSL"
     gene            2230..>2597
                     /locus_tag="VF272_00605"
     CDS             2230..>2597
                     /locus_tag="VF272_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX7259416.1"
                     /translation="MRYAALLRGINVGGKAKVAMPKLKAVFETLGCTDVITYINSGNV
                     VFLDGRSSKELVPLIESAIAEEFRLAIRIVLRDSMAITKLCKEIPSSWTNDSVQRTDV
                     LFLWEEIDDPAILQKITINPA"
BASE COUNT          682 a          613 c          608 g          694 t
ORIGIN      
        1 agcccttcgg cagccaggat cggctttcaa gccaattgtg tacgctcggg caattgagga
       61 taaaacgatt acggcggcaa ccgtgcttaa agacagacct cgcacgtttg cagccaatta
      121 ccgtccccaa aattatgatc gcaagttccg aggagacgtg ttagtgcggc gggctctcgc
      181 aaactcgttg aacgtaccgg ccgtggaagt tatggaaaaa gtgggaacaa ataatgtctt
      241 aactatggcg gggaatatgg gtattacaac cctaaaaagt gtttcggatt acggcccatc
      301 ccttgtactc gggacggggg aaacccgcct aacggagtta acgagcgcat atggagtatt
      361 tgctaatggc ggtaaaagaa ttccgataac gacaataacc aaggttcaaa acaagcaggg
      421 gaaaactatt tttcgtaaca ggcaaaaatc tacccaagta attaatccgg ctgttgcctt
      481 tattgtatct tcaatccttt cggacaatgc tgccagggcc gagcttttta gtaaccggct
      541 caacgtcaat cgtccggcgg cggttaagac cggcacaact gaagattatc gggatgcatt
      601 aaccgttggc tatacaccca gcgtggtggt tggtgtgtgg gtagggaaca acgacaacac
      661 acccatggac aatatagcgg gctctcttgg tcccgcaccg gtgtggcgat cccttattga
      721 gcgttatagt aaagggttgt cgtctgagtc attcaagcct cccggcaacg ttgccgcttt
      781 gtcggtctgc cgcagtaacg gcttgaaggc gttgcgaccg ggcgccgcca cctacactga
      841 atatttcctt gagcgaactg agcctacgca atcctgcaat gttccggcgc cagcacctaa
      901 gcccaagaat gataaaaacg acgtcattaa gccaaaagaa gaagaaaggc caggcgaaga
      961 acccttggat gacacccctg tggaaccaac accgtctcct acgcctgagc caactccgac
     1021 acccctacct ccgccacaac caccagtaac gccctagaat ccgtatcgtc tttcggcaaa
     1081 cctatataca tcattgacca ttttcttaac ttccaactcg gctaaatcat tgccagagtt
     1141 gacgcggtgc atccctggcg gcttatagta aatattctcg cacatcaatc ctccgattat
     1201 aacgactgga tgggtgtaga tgatttcccg aatcgctgca gcgccgaagc gattcatatt
     1261 gaattggcac attcctacaa cttgatgctt ggggaaaagt tcatcattta tgcgggcttc
     1321 gtattctaat aagtgctcag tttcggtatg actatcatcc gctaattgcc aggtcatttc
     1381 cgcacaacct cgaacagccc taaagccttt tgatgttgct tgatgtgagg cttcagcaaa
     1441 tacttttatg gttgtatcgg gactgaaaga accattctcc aaatacgatc cgtcctttgt
     1501 gcctagaaca agctgctcgg tttccagcat attttcagta gtaataccgg cgtgctccaa
     1561 ggcctggaga acagcttcgg gcgtattgtc gtcgcttatg taaatacatt gatcgtgctt
     1621 gcgtagcccg gcctcaataa atgctgcagc actcgctaaa ccttcttggc tatcacgata
     1681 gagcaggcat acatggtcgt gtacttccac attttggatt gtctggagaa aacgcttatt
     1741 tggccgtggg atctcgtgta atatattttg caccaactta ttgtagtggg attgcggcat
     1801 gcttactgct gctggattga acaaaaaaac tgtatgaaaa tatcgtattt ttagttactg
     1861 ccacaatgct atcgggggct tgtcttgggg ccgcattttt acagcttgct gctcttgtca
     1921 tccaaatgta gctatgattt gtattggttc atatgctgat ttgctatact ttctttattg
     1981 ataagataaa agcaggaata ttcgatggca aaatcgacaa aagtaatgaa tcacggttca
     2041 ttaggcggcg tatttttagc tacctatatt ggagcggcgg tatattttct gcaacatact
     2101 caaggctttt gggggaccat ctgggcgctt attgaagcac tattttggcc tgcatttatt
     2161 atctaccacg tgctcactca gcttcaggtg tcgtcgctct aacgatctga tttttgctac
     2221 catataccta tgagatacgc tgcattgctt cgaggtatta atgtaggcgg caaggctaaa
     2281 gtggcaatgc cgaaacttaa agcagttttt gagacgctcg gttgtaccga cgtgataacg
     2341 tacataaatt cgggtaacgt agtattcctt gacgggcgct catcgaaaga actggtgccg
     2401 ctgattgagt ccgctattgc cgaagagttt aggctagcta ttcgcatagt cttgcgagat
     2461 tcaatggcaa ttaccaagct ctgcaaggaa ataccatcta gctggacaaa tgatagcgtg
     2521 caacgaaccg acgtattatt cctatgggaa gaaattgacg atccagccat tttacagaaa
     2581 attaccatta acccggc
//
LOCUS       DASVGB010000022         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_404040, whole genome shotgun sequence.
ACCESSION   DASVGB010000022 DASVGB010000000
VERSION     DASVGB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404040"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VF272_00610"
     CDS             <1..885
                     /locus_tag="VF272_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259417.1"
                     /translation="EHPEPSPEELIIDGLAETVEPKETAFFSVIGRELGLTPEETATL
                     EGRLSERPGFLVDLYAEQQYLATHVGETIPSRQDLTKYQPEIQAWLNDSERSNRESRI
                     HGPAHFMRVLIDTKLLSCLIEPQLPAKLSELSVKDVPEAYDHMVSTIDHEALAAFAIG
                     HDWARHDDESEDPDHGARAAESLEDPNVFPDLEDASRILAQALMTGHSREDEPTDVAL
                     EDRVARDADLLDRHRDYEGPDPQFIRLHATARIDAVAFTLAVMSDFLIEQKQEDQVEA
                     VLKTAGALGIVKPEEARR"
     gene            960..1277
                     /locus_tag="VF272_00615"
     CDS             960..1277
                     /locus_tag="VF272_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259418.1"
                     /translation="MELRIAQQQRLPLWVHAWQSAALVVAWLAGSALVLPFLVILAEV
                     QPSADIYNAGLVAMIIGYGIATTFYARWLHRRGTVAGAIALYILVALYAMGQIPHLVR
                     IVT"
     gene            1592..>2149
                     /locus_tag="VF272_00620"
     CDS             1592..>2149
                     /locus_tag="VF272_00620"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7259419.1"
                     /translation="MAEAKQAAQFEENLHAMRHSMAHILATAVQELYPGVTFGVGPVI
                     ENGFYYDFDLNDALTPEDLPKIEATMKEVIKAGERFEHQDMAIDEAIAYFKKRRQPYK
                     VELLHDLKVHGTTVAKDINRDQLGIKNEEKITTVSLYTNGNFTDLCRGGHLETTKQAG
                     AFKLTKISGAYWRGKSENPQMQRVYG"
BASE COUNT          520 a          541 c          600 g          488 t
ORIGIN      
        1 gagcatccgg aaccgagtcc ggaagaattg atcattgatg ggcttgcgga gacggttgag
       61 ccaaaagaaa cggcgttttt cagtgtaatt ggccgggagt tgggcctaac gccggaagag
      121 accgccacgc ttgagggcag gctctcggag cgacccggat ttctcgtaga tctgtatgcc
      181 gagcagcaat atctagcgac gcatgtaggc gagaccatcc cgagccggca ggacttaacc
      241 aaatatcagc cggaaattca ggcctggctt aatgattcgg agcgctcaaa tcgtgaatcc
      301 agaattcatg gcccggcaca ttttatgcgg gtgcttattg atacaaaatt actttcatgc
      361 cttattgaac cgcagcttcc cgcaaaactt tcagagttaa gcgttaagga tgtcccggaa
      421 gcatatgatc acatggtttc gaccatcgat catgaagcat tggcggcctt tgcgattggc
      481 cacgattggg cgcggcatga tgacgagtcc gaagatccgg atcatggagc gcgggcggcc
      541 gagtcactgg aagatccgaa cgtattccct gacttggagg atgcgtcccg tattctggca
      601 caagcgttaa tgacggggca ttcaagggaa gacgaaccga ccgatgtggc actggaagac
      661 cgagttgcga gggatgctga tctcctggat cggcatcgcg actacgaagg tcctgaccct
      721 cagttcatcc gtctgcatgc aacggcacgc attgacgctg ttgccttcac ccttgctgtc
      781 atgagtgact ttttgatcga gcagaagcag gaggaccaag ttgaggctgt actgaagacg
      841 gctggagcgt tgggcatcgt gaaacccgaa gaggcgcgcc gatagctagt aaattgagtg
      901 taatttgtta ttataattcg gctgttccaa cttcaaccgc tccttggagg ttcatcccaa
      961 tggaattgcg tatcgctcag cagcagcgct tgccgctgtg ggttcatgcc tggcagtcgg
     1021 ccgcattggt ggttgcctgg ctcgccggat cggccctggt gctgccgttt ctggtcattc
     1081 tggccgaagt ccagccttca gccgatatct acaacgcggg cttggttgcc atgatcatcg
     1141 gttacggcat tgccaccacc ttctacgctc ggtggctgca tcgacgtggg accgttgccg
     1201 gcgccatagc gctctacatc ctggtggctc tctacgccat gggacagatc ccgcacctcg
     1261 tccgcatcgt gacatgacca aaagaccagc gcctccacca accaccccgg ctgcaagagc
     1321 ggactgctgc cggggcttgg ggtgaaggga gtaccgtcct ttcacccccg taaccttgtt
     1381 aatatcagcc gatttgctat gatgaagcca ttgaaagttc aatagggcag caatcgggag
     1441 gtaacggccc cgtagtcccg ccgccggtgg cgttccatcg gcacgcaaag agccccttaa
     1501 ctaaggggaa gtttggtgga accgcccggt atgaatgggt ccaaacgcgc atacctatgc
     1561 gtgttttttg ttatctaaga ggagaagaaa aatggctgaa gcaaagcaag ccgcacaatt
     1621 cgaggaaaac ctgcatgcca tgcgccattc gatggcccat attttggcga ccgctgtcca
     1681 agagctctat ccgggggtaa cgttcggtgt tggaccggta attgaaaacg gcttttatta
     1741 tgattttgat ctgaacgatg cgcttacccc tgaagatctg cctaagatcg aggccacaat
     1801 gaaagaagtc attaaggctg gcgagcggtt tgaacaccaa gatatggcta tcgatgaggc
     1861 catcgcttac ttcaaaaagc ggcggcagcc gtacaaagtg gagcttttac acgatttgaa
     1921 ggttcacggc actacggtgg ccaaagacat caatcgagat cagctgggca ttaaaaatga
     1981 agaaaaaatt acaacagtta gtctctatac taatggtaat tttaccgatt tgtgtcgcgg
     2041 cggccatctg gaaaccacta aacaggcagg agcttttaaa cttactaaaa tttctggagc
     2101 ctactggcga gggaagtcgg aaaatccgca aatgcagcgg gtctatggc
//
LOCUS       DASVGB010000023         6553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_269856, whole genome shotgun sequence.
ACCESSION   DASVGB010000023 DASVGB010000000
VERSION     DASVGB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269856"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF272_00625"
     CDS             <1..491
                     /locus_tag="VF272_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7259420.1"
                     /translation="KRVRHIARRMYDIVAGYTNGNLLIAAGVGILTSALLVAFGVPYA
                     IPLGIFSGIMTLIPIAGMIIAMIAIGGVALFTSVTAAIVVVGFLFFYSIIDGNIVRPL
                     VFGRTIQMSPLMILLAILFGTAFGGLIGALVAIPVTACLGVLFEQVVDDLESQAKHPK
                     EA"
     gene            complement(501..1907)
                     /gene="cysS"
                     /locus_tag="VF272_00630"
     CDS             complement(501..1907)
                     /gene="cysS"
                     /locus_tag="VF272_00630"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7259421.1"
                     /translation="MKLYNTLTRTKEAVTPITPQHVSLYTCGPTVYHYYHIGNLRNAV
                     FNDTLKRVLLAEDNHIHHVMNITDVGHLTGDTDHGNDKLQERAAKEGKDVWEVAQYYT
                     ETFFNDMNRLNVLEPTRYVKATETIEQQIAMILELINTGFAYQTEEAIYFDTTKLKDY
                     GKLSGQNLKEKQVGVRAEVVTDPDKRHPQDFALWFFTVGRFEDHAMRWESPWGEGFPG
                     WHLECSAIIEAEFGDTIDIHTGGVDHIGTHHTNEIAQSEASHNGAPLAHYWMHIEFLL
                     VDGHKMSKSLENTYTLNEVTARGYDPMALRLLYLQSHYRTQQNFTWDALDAATNYLHR
                     LQAWADGQFQAGAGTLSDDSFHDFCRDILAKLSDDLNTPEAMVIMSQLLDDFESNLVM
                     PTAEQVGQIDALFGLGLSKRLNITPEQEAIILEREGARQAKDYELSDHARSLLKDQGI
                     ELDDTPSGPRWYRRPQTS"
     gene            complement(1882..2904)
                     /locus_tag="VF272_00635"
     CDS             complement(1882..2904)
                     /locus_tag="VF272_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX7259422.1"
                     /translation="MSTLESAITALDALVTEATTILILQPEKPDTDSLTASLALEQIF
                     GDMGKEVIMYSQDAVPRYLAYFEGADRVHDIFPDHFDVSIIVDTGGAQQLARTLEKYQ
                     GRIIKKPIAIIDHHPNREPMPFPTMDVIDPHATSTAEVLVTIAKQLGWTINKEAASLM
                     VPGILADTRNLSIATVGSGTFRTVADLIDLGANMYEIHEAYRASDRLTPELVNLKGRL
                     LSRMELHSNGKIALVVVTPEELKHYAEMHDPADLVIYDMQNTLGVAVAVVMRHYGGQN
                     NKIKISTRASMPVAAKACKAFGGGGHDRAAGCQVNDTPISEVKPAFITELTKQIQDYE
                     ALQHAN"
     gene            complement(2901..3500)
                     /gene="recR"
                     /locus_tag="VF272_00640"
     CDS             complement(2901..3500)
                     /gene="recR"
                     /locus_tag="VF272_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7259423.1"
                     /translation="MQTIPHSIAQLIDEFAKLPGVGPRSAERLTFFLLRHNTGQETQL
                     AQALQNLRSQLEVCKQCHNYSEGDHCVICKSLRRDQSIITVVEEPLDVVAIEKTGLYE
                     GVYHVLGGVISPMDGIGPAQLSISDLIERARSNDVTEVVLATNPTTEGEATALYIRKQ
                     LEPTAVTVTRLARGLPVGGDLEYADQITLGRALQERQAF"
     gene            complement(3515..3799)
                     /locus_tag="VF272_00645"
     CDS             complement(3515..3799)
                     /locus_tag="VF272_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7259424.1"
                     /translation="MFDKAKTLYELKKIQSALAKETIEIETGDGAVKVVINGEQKIKK
                     VELKPELIDGDYAKLEKWIESAVTQAITRSQQLAAAKMKSVAGGLGLPGL"
     gene            complement(3835..5307)
                     /locus_tag="VF272_00650"
     CDS             complement(3835..5307)
                     /locus_tag="VF272_00650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7259425.1"
                     /translation="MNQIAKRIIPGWGLVVFLVLAGAAVRWWNLGKASIWHDEGFSIM
                     MAQLPLSEILERTARDVHPPLYYMALHYWISVFGSSEVAVRSLSMVATLLIIPLTYCL
                     VRRLWNERGAQLAAVFVCFGPMLVRYGQEARMYGLMAFLVLLTVYFLVRAMERNQARW
                     WIGFSLSLAAAMYTHYYSVFIIPAVLVYVTLNTNRQKQSGWWNPRWWLSSLGALLLFA
                     PWLPSAYGQVTRQKYFDWIPSADAATLPNTLLQFLAYGYPPTLSMAIKLAIGGMFIGL
                     MVLAWKAAGRQRTNLLVFGVYALAGPVLVYAASLSGSPYLERYFIYAAVGFYILLALM
                     ITTLGRRSQLAVAIYVLVLFGVGSYNIHTQYTHDMRSAAAYIQQQYQPGDYLLSDVLL
                     TYFDFSYYNQTGAPAHVWQEGGVNGYGESSLIYTKADEVVVRRLETIQPASGRVWLIS
                     KLDKQGSVAKVPVHWTAAGPKRIFGDNAVQQYLMAPRGQK"
     gene            complement(5291..6055)
                     /locus_tag="VF272_00655"
     CDS             complement(5291..6055)
                     /locus_tag="VF272_00655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7259426.1"
                     /translation="MSQPALSIIIPAYQEAARISLTLIRLSAFLKKHAYDDTEVLVVV
                     ANGTDETATLAAAKAGLFSKFRVVYAGPRAGKGRDVRTGIMEARGRFKLFMDADLATP
                     LHHLHTVQGLIKDNPEVVIGVRDLHASHTGLRKWLSNVGNRVVRLVLLPGITDSQCGF
                     KLFRSDVAAELFGRQRILGWGFDMELLTIARQRNYRINTIAIHDWHDQPDGTFERTMG
                     PAMFQTLGELIVIIWHRSTGAYRNKRFVYQRYEPNR"
     gene            complement(6052..>6553)
                     /locus_tag="VF272_00660"
     CDS             complement(6052..>6553)
                     /locus_tag="VF272_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259427.1"
                     /translation="AALGTLYILDQWRAVFPLNAPARMLGSTAVVILLTLTVYQGYTH
                     YFVAWANSPETYKAYQEESASLAQYYNTHPFKGKRYAIADDYAMKPVAFLTHKKASYH
                     RLETKAVAALPLEPSGAKEFSILSGDTSEVLPHLERKYPKLQRSPHYSTFDGRELFVI
                     YTVPAS"
BASE COUNT         1545 a         1709 c         1667 g         1632 t
ORIGIN      
        1 gtaaacgggt ccgccatatt gcccgccgga tgtatgacat tgtggctggc tataccaacg
       61 gcaacctgtt gattgcggcc ggtgttggca ttctgaccag tgccttactg gtggcgtttg
      121 gtgtccccta cgctatcccg ctcggcattt ttagcggcat aatgacgctc attccgattg
      181 ccggcatgat tattgcaatg atcgccattg gcggcgtagc actgtttacc tcggtaacgg
      241 ccgcaattgt ggtggttggt ttcttgtttt tctatagcat tattgacggc aatattgtgc
      301 ggccgctggt atttggccgg accattcaaa tgtcgccctt aatgattctt ttagcgattt
      361 tatttggtac cgcctttggc ggactgattg gggcgttagt ggcaatccca gttaccgcgt
      421 gtttgggcgt actgtttgaa caggttgttg atgacctgga atcccaagcc aagcacccga
      481 aagaggccta ggttcgccgg ttagcttgtt tgagggcgtc ggtaccagcg cggtccgcta
      541 ggtgtgtcat cgagttcaat tccttggtct ttcaatagtg agcgggcatg atcagaaagt
      601 tcatagtctt tggcctggcg agcaccttcg cgctccaaaa taatagcttc ctgttccggt
      661 gtaatattga ggcgcttgga gagacccaat ccgaacagtg cgtcaatctg ccctacctgt
      721 tcagcggttg gcataaccag gttggattca aaatcatcaa gaagctggct cataatgacc
      781 atagcctccg gcgtattcaa atcgtcgctc agttttgcca ggatgtcacg gcaaaagtcg
      841 tgaaagctgt catcgcttaa ggtgccggca ccggcttgaa actgaccgtc ggcccaggct
      901 tggaggcgat ggaggtagtt ggtagccgca tctagggcgt cccaggtaaa attctgctgg
      961 gtacggtagt ggctttgcag atacaacaaa cgtaaagcca ttggatcgta gcctctggcg
     1021 gtcacctcgt tcagggtgta ggtattttcc agcgacttgc tcattttgtg gccatctacc
     1081 agcaggaatt caatatgcat ccaataatgt gccagcgggg caccattatg cgatgcttcg
     1141 ctttgggcaa tctcattggt atggtgggtg ccaatgtggt cgacgccgcc ggtatgaatg
     1201 tcgatagtgt cgccaaattc agcttcaata atcgccgagc attccaggtg ccagccgggg
     1261 aagccttcgc cccaggggct ctcccaacgc atagcatgat cttcaaaacg gccaacggta
     1321 aagaaccaca aggcgaaatc ttgaggatgg cgcttatcag gatcggtcac cacctcggcg
     1381 cgaactccta cctgcttctc cttgaggttt tggccgctga gcttaccata atccttcagc
     1441 ttggtggtat caaaatagat ggcttcttca gtttgatagg caaacccagt atttattagc
     1501 tccaaaatca tggcaatttg ttgctcgatg gtttcagtag ctttgacata gcgggtaggt
     1561 tctagcacgt taagacggtt catatcgtta aagaaggtct cggtatagta ctgggcgacc
     1621 tcccagacat ctttgccttc cttggccgcc cgttcttgga gtttgtcatt gccatggtcg
     1681 gtgtcgccgg taagatggcc aacatcagtg atgttcatga cgtggtggat gtggttgtct
     1741 tccgccagta gcacccgctt aagggtgtca ttaaagacgg catttcgcag gttgccaata
     1801 tgataatagt ggtagaccgt cggaccgcag gtatacaggc tgacgtgttg aggcgttatt
     1861 ggcgtaaccg cctccttggt tctagttagc gtgttgtaga gcttcatagt cctgtatttg
     1921 cttagttaac tcagtaataa atgcgggttt tacttcagaa attggcgtgt cattcacttg
     1981 gcagccggct gcacgatcgt gaccgccgcc gccaaaggct ttacaggcct tagcggctac
     2041 cggcattgag gcgcgggtgg aaatcttgat cttattattt tggccgccat aatgacgcat
     2101 taccaccgcc accgctacgc ccagtgtgtt ctgcatatca taaatcacca aatcggccgg
     2161 atcatgcatt tcggcatagt gttttaactc ctccggcgtt accacaacca acgcgatttt
     2221 accgttggaa tgcagctcca tgcgcgacag caaccgccct tttaggttga ccagctcagg
     2281 agttaagcga tcgctcgccc ggtaggcttc gtgaatctca tacatgttgg cacctaaatc
     2341 aatcaagtcg gcgacggtcc ggaaggttcc ggagccgacg gtagcgatgg agaggttacg
     2401 ggtatcggct aaaatgccgg gtaccatcag actggcagcc tctttattga tcgtccatcc
     2461 gagctgctta gcaatggtta ccagcacttc ggcggttgag gtagcgtgcg ggtcgatcac
     2521 atccatggtt ggaaatggca ttggttcgcg gttaggatga tggtcgatga tcgctatcgg
     2581 ctttttgata attcgccctt ggtatttctc taaagttcgt gccagctgct gtgctccgcc
     2641 ggtgtcgaca ataatactga cgtcaaaatg gtctggaaag atatcgtgta ctcggtcggc
     2701 accttcaaaa taggccaggt agcgcggtac ggcgtcttgg gagtacataa tgacttcctt
     2761 gcccatgtcg ccaaaaatct gttccagcgc caggcttgca gttaagctat cggtgtccgg
     2821 cttttccggc tgcaggatca agattgttgt ggcctcggta accagtgcat cgagagcggt
     2881 tatggcggat tctaaggtgc tcaaaacgcc tgccgttctt gcagggcgcg gcccaaggtg
     2941 atttgatcgg catactcaag atcgccgcct actggtaagc cgcgggctag ccgagtaacg
     3001 gtaactgcgg taggttccag ctgtttgcga atgtagaggg cggttgcctc gccttcggta
     3061 gtagggttgg tagccagaac cacttccgta acatcgttac ttcgcgcccg ttcaataaga
     3121 tcactaatgc tgagttgagc cggcccgata ccgtccattg gagaaataac accgcccagc
     3181 acgtgataaa caccctcata taagccagtc ttttcaatcg caactacgtc cagcggctct
     3241 tctaccaccg taatgatcga ctgatcgcgg cgcaagcttt tacagattac gcaatgatcg
     3301 ccttcactat agttgtggca ttgtttgcag acctctagct gagagcgcaa attctgtaac
     3361 gcctgagcca gttgagtttc ctggccggtg ttatggcgca gcaagaaaaa agtcagccgt
     3421 tcggctgagc gcggaccgac tccagggagc ttggcaaact catcgatcaa ctgggcgata
     3481 gaatgcggga ttgtctgcat acgggaaaaa ccgcctaaag tccgggtagt ccgagcccac
     3541 cggctacact cttcattttg gcagccgcca gttgttggct gcgggtaata gcctgggtta
     3601 ccgccgattc aatccacttt tccaattttg cataatcgcc gtcaatcagc tctggcttca
     3661 attcaacctt tttgattttt tgctcgccat taatgaccac ttttacggca ccgtcgccgg
     3721 tttcaatttc aatggtctcc ttggcgaggg ccgactggat ttttttcagc tcatacaggg
     3781 ttttggcttt atcaaacatc gaaaactcct tcttatctgc tattgtagcg cactttactt
     3841 ctggccgcga ggtgccatta ggtactgttg aacggcgttg tcgccaaaaa tgcgcttggg
     3901 tccggcagcc gtccaatgaa ccggaacctt ggctacagac ccctgtttat ccagcttgct
     3961 gattaaccac actcttccac tggccggttg aattgtttca agacggcgaa ctaccacttc
     4021 atctgccttg gtatatatca gacttgattc cccgtagcca ttaacgccgc cctcctgcca
     4081 aacatgagcc ggcgccccgg tttggttgta gtagctaaaa tcaaaatatg taagcaaaac
     4141 atctgagagc agataatcgc cgggttgata ctgctgctgt atgtatgcag ctgctgaacg
     4201 catatcgtgg gtatactggg tatgaatgtt gtagctgcct accccaaaca gcaccagcac
     4261 ataaattgcc accgccagct gactgcggcg tcccagcgta gtaatcatta aggctagcag
     4321 aatgtaaaat cccaccgctg cgtaaataaa atagcgttcc agatacggcg atccgctgag
     4381 tgacgcagcg tacaccaata ccggaccggc aagtgcatag acgccaaata ccagcaggtt
     4441 tgtgcgttgc cgacccgctg ccttccaggc cagtaccatt agcccgataa acatgccgcc
     4501 aattgccagt tttatcgcca tactcagtgt cggcggatag ccatacgcca agaactgcaa
     4561 taaggtgttg ggcagcgttg cggcatcggc cgaagggatc caatcaaaat acttttggcg
     4621 cgttacttgg ccataggcgc ttggcaacca aggcgcaaac agcaacagtg ctcctaggct
     4681 gctcaaccac cagcgcggat tccaccaccc gctttgtttc tgccggttgg tgttgagcgt
     4741 tacatatact aagactgccg ggataataaa aacactgtag taatgagtgt acatggcagc
     4801 ggccaaacta agactaaacc caatccacca gcgagcctgg ttgcgttcca tggcacgcac
     4861 caggaagtag acggtcagca aaaccagaaa agccatgagg ccatacatcc gagcttcctg
     4921 gccataacgc accagcatcg gtccaaagca aacaaacacc gccgctagct gtgctccgcg
     4981 ctcgttccac aggcgccgaa ccagacaata ggtcagcgga ataatcagaa gcgtagcaac
     5041 catgcttaac gagcgaaccg ccacctcgct ggaaccaaac acgctaatcc agtaatgcag
     5101 cgccatatag taaagcggcg gatgcacatc tctggccgtt cgttccagaa tttcacttaa
     5161 cgggagctgg gccatcataa tcgagaaccc ttcatcgtgc cagatactgg ccttacctag
     5221 gttccaccag cgcacggccg ctccagcgag cactaaaaaa acaaccagcc cccatcccgg
     5281 gatgatacgt ttagcgattt ggttcatatc gctgatatac aaaccgtttg ttccggtaag
     5341 cgccggttga acggtgccaa ataataacta taagctcgcc aagggtttgg aacatagccg
     5401 gccccatggt gcgctcaaag gtgccgtctg gttgatcatg ccagtcgtga attgcaatcg
     5461 tattaatacg ataattccgt tggcgggcga ttgttaaaag ctccatatca aacccccaac
     5521 ccaatatccg ctgtctgccg aatagttcag cggccacgtc gctgcgaaac agtttaaacc
     5581 cgcactggct atcggtaata cccggcaaaa gtaccagacg cacgacccgg ttacctacat
     5641 tagagagcca tttgcgtaaa ccggtatgac tggcgtgtaa gtcgcgcacg ccaataacta
     5701 cctcgggatt gtccttgatg agaccctgga cagtatgcaa gtgatgcaag ggcgttgcca
     5761 aatcggcatc cataaacagc ttaaagcggc cacgggcttc cataatcccg gtacgtacat
     5821 cgcgcccttt tccagcccga ggcccggcat aaactacccg aaacttacta aataaaccgg
     5881 ccttagcggc tgccagtgta gctgtttcgt cggtaccgtt agcaaccacc accaacacct
     5941 cggtatcatc ataagcatgt tttttcagaa aagctgacaa acgtataaga gttaatgaaa
     6001 tgcgggctgc ttcctggtaa gccggaataa taatgctcaa tgctggctgg ctcatgacgc
     6061 tggaacggta taaataacaa acagctcgcg gccatcgaag gtggaataat gtggactgcg
     6121 ctggagcttt ggatacttac gctccaaatg cggtaatacc tcggaagtgt ctcctgaaag
     6181 aatgctaaac tccttggcac cgctaggctc aagcggcaac gccgcaaccg cctttgtttc
     6241 cagacggtga tagctggctt ttttatgcgt taaaaaagcc accggcttca tggcataatc
     6301 gtcggcaatg gcatatcgct ttcccttaaa cggatgcgta ttgtaatact gagccaagct
     6361 ggccgactct tcttgatagg ccttatacgt ttcaggggaa ttagcccatg ccacaaaata
     6421 gtgcgtgtag ccttgataaa cggttagtgt caacagtatc accaccgctg tgcttcctag
     6481 catgcgagcc ggagcattta aggggaaaac ggctcgccat tgatccagta tgtacaacgt
     6541 tccaagtgcc gct
//
LOCUS       DASVGB010000024         9434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_544638, whole genome shotgun sequence.
ACCESSION   DASVGB010000024 DASVGB010000000
VERSION     DASVGB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544638"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF272_00665"
     CDS             complement(<1..408)
                     /locus_tag="VF272_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259428.1"
                     /translation="MQEKNLILAIDDVEPGLVQAVKKLSKTLGRELHGLVLVDLNYPA
                     IRERARDTSGLFKEIICDFDDPNVLQKHLKPHLDSLLAVTLRHESSVQSLRKVVPFLP
                     FVPTPSESSLLWSTEKHLMRDRLHNYDSRLVPQY"
     gene            complement(442..3033)
                     /locus_tag="VF272_00670"
     CDS             complement(442..3033)
                     /locus_tag="VF272_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259429.1"
                     /translation="MFHQRLSHRAFFIVAGLVGIVPVAWAAPAQASEWRVAAFSGASV
                     SVVAVNQRSSHEMAAIVNGNLQLSHDAGVTWLPGLLSVPARTISFDLVYAGRLYAGTG
                     SGIYISDNGGMTWQPFGASITRRKIVTALQTTEEYVYAALYDGAGTPVRVWRFDASGE
                     AQDMNLPSDNIAAFDYDYASHTLYVGAAGGVYQSSNNAASWSGGGRGAGLFTAAVAVK
                     EGLIWQLSADGLFRSNDNGASWNKLAGPGNINGTFYGHDMHLRGLAVSGNQAFYGAWS
                     IGFPYRFLVTYDGSAARSLLNVKIASVARHANRIWAASDNGLWANDIQGLSLPRVYRP
                     VLLIPGILGSLPTMESLASQLPRVFTGFWDRTYQTPLVLDPVRHTYDGLLQALQSRGF
                     SLDTTLFVFPYNWMQDNAQTAAQLSQKLHDIRQKCHCSQVDVVAHSMGGLVARRYIQS
                     TAYHHDIRNLIQLGTPNAGSVAAYEVWEGGVASGLKPDSDNLLLNFIASALNNPDIAV
                     PRAELLRRAIPSIGQLLPVFDYLSGRRYPAGYPVNPFLESLNLPADIARLKERVAFHV
                     VGAANRSTLRELTVAAVNSSAIVWPHGSIVSPVFGMGDGTVLQASLEAAAKSSLMLSV
                     GHLQLVSDAAPFILQIIMGEYVPDVTIPLLPPQRFLGVYAAGPVALTVTSPSGQIIDD
                     SKIGIAGAYYSGSTVKPQLAFIPRQGVGDYAITLKGTGSGAFQVGAAELNPDTMGETA
                     ISTYLSGESSVLATIAPGEIRRYIYKSQDQQLIAIDSFRSGIQPESKSDRIALSEPVL
                     ASFTHSPVFGYAANAFRADPKKAQAVTRHNHIERRNRVVLVAAAASLLLLFMTVLLIA
                     IHRSKKV"
     gene            3186..3605
                     /locus_tag="VF272_00675"
     CDS             3186..3605
                     /locus_tag="VF272_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7259430.1"
                     /translation="MADVKSIKRLRVYQTSRELEDSVYMLVKALPETEFYALGNDLRR
                     SSAAISHYLMQCHRHYSHNLKLEALHLARTEAETLQNLLLSHDAKGYGSTDHLGDACI
                     SVIKQCWGLIKYFKQRQAEQSIAASVRASDELVASRS"
     gene            3866..4309
                     /locus_tag="VF272_00680"
     CDS             3866..4309
                     /locus_tag="VF272_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX7259431.1"
                     /translation="MKCPYCRAESTDVFNSRPTKFGNQIWRRRRCLSCRESFTTYETA
                     DLGFITICKASGKPQPYSRATLFASVFEAFLLTPQKQIEVDAVTDTVEAKLLDLQQPT
                     ITSSEVAEVVLQTLKHYNTSAFIRYLSNQTDLVNEAQLRKELKKY"
     gene            complement(4393..5094)
                     /locus_tag="VF272_00685"
     CDS             complement(4393..5094)
                     /locus_tag="VF272_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX7259432.1"
                     /translation="MADKITIQLQSRDSVRKTNRNLRKSGNTPAIMYGHGSKAIAVTV
                     LAKELEQVYAQAGGNRIVSLKLDDGKLNNALIHDVQLDSRTGHIIHADFFLIRMDEKI
                     KAEIPLHVIGESTAVYQQEGTLTRPIETVEVEALPADLPESIEVDISVLDDFDKNITI
                     GDLKFPANVEVLTPPETLVATVVAPRSDEELEELDAAVESELPEGVAEDQEVIVEDSE
                     QTRKEPENRSDKSQN"
     gene            complement(5301..5630)
                     /locus_tag="VF272_00690"
     CDS             complement(5301..5630)
                     /locus_tag="VF272_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259433.1"
                     /translation="MSRRHLQDIINNLQEFYKCPSCDTSYCLDDIRYLGELNQYCFVQ
                     LNCHECSLPVLATVATSGKTTKRSKTDLRSAEEAKFASMGTISADEIAEFHRYISRKR
                     TGFSKKR"
     gene            complement(5697..6749)
                     /locus_tag="VF272_00695"
     CDS             complement(5697..6749)
                     /locus_tag="VF272_00695"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX7259434.1"
                     /translation="MIYLVAFLVSFGLALLLTPFVRMLALRYQIVAQPVAIKSHALDN
                     RVVPYLGGLAIVASFVIGALTFLPMNRQLAALLIGGIMLAVLGAIDDVRGLSAKIRLF
                     WQFLAACVVLAGGIGIVSITNPLGGVIDLTAGRFAVDWGILHFHITPIANALSILWIV
                     GMVNTVNFLDGLDGLAGGVSAIAAIILFALAISPEVNQPLVALLAIILAGSALGFLPF
                     NFFPAKIFMGDSGAYFLGLTLALLAIYSGGKLATLSLVFGFTIIDAMWAVVRRLRRRV
                     HPFTSDREHLHHLLLKAGLSQRGAVLSLYMLAVTFGVVALQTQSFTKLVALIVLLATM
                     VVLIASLIRIGSRRPA"
     gene            complement(6746..8443)
                     /locus_tag="VF272_00700"
     CDS             complement(6746..8443)
                     /locus_tag="VF272_00700"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7259435.1"
                     /translation="MTEAVRTKYRYATDQRWFIRMLEIIPGAITWTLLIGPIILSFFL
                     PIYVSYFIIAFDLFWLLKALRMSTYLVRGYRRLRDHQALNWRERLNWLEDPEKHIKAI
                     DRRIQDLLRRYPKVQRRFFWNRSHTKQRRGYVQALSDLSSLKEIESHQTAIMPPSSIC
                     HLVIIATYNESRDVLEPTVKSLLEADYPSERMIVVIAYEERGGQAVKETAEDLIASYG
                     KRFNYASAIMHPDNRPGELRGKGANITYAARQATADIIERGIDPEHVVVTTLDADNKP
                     DPQYFAYLTYVYALDPNRVHKSYQPIPMFLNNIWDAPAPMRVIAAGNSFYQIMEMMRP
                     HRLRNFSSHAQPLRALIETDFWSVTSPVEDGHQFWRSYFIFNGDYAVVPLYIPIYQDA
                     VLAETYPKTFKAQYIQLRRWAYGISDFSFVVRNCIRNNKIPLGSKLIQTGRLLEGHIS
                     WATAVLLITFVAWLPLFLNPQFSQYGLAHELPVIASHLQSIALIGLFVMASISIISLP
                     PRPERYGHRRSLAMVAQWLLFPITGIVFNALAAIDSQTRLMLGRYLGFNVTVKSRRK"
     gene            complement(8440..8835)
                     /locus_tag="VF272_00705"
     CDS             complement(8440..8835)
                     /locus_tag="VF272_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259436.1"
                     /translation="MNLNAPLQVLRRLNLSDLRQHPMMKDPVSLTALGISLVLNILTL
                     GVMLVKVHQVDYPVPVHYLSLIGFDQVGPWYQNYRIAAFGFAVTIINALLAGQSFQRN
                     RLTSFFLLIGAVAVGLLCLVIGSEFAAII"
     gene            9051..9126
                     /locus_tag="VF272_00710"
     tRNA            9051..9126
                     /locus_tag="VF272_00710"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9084..9086,aa:Thr,seq:cgt)
     gene            9146..9219
                     /locus_tag="VF272_00715"
     tRNA            9146..9219
                     /locus_tag="VF272_00715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9178..9180,aa:Gly,seq:ccc)
BASE COUNT         2484 a         2456 c         2336 g         2158 t
ORIGIN      
        1 gtactgcggc accaaacggc tatcataatt atgcaaccgg tcgcgcataa gatgcttttc
       61 ggttgaccac agcagggacg actctgaagg agtgggaaca aatggcaaga acggcaccac
      121 cttgcgtaat gactgcactg aactttcgtg gcgaagcgta accgccaaca gtgaatccag
      181 gtgaggctta agatgttttt gaagcacatt cggatcatcg aagtcgcaga tgatttcctt
      241 gaatagccca gaggtgtctc gagcccgttc acgaattgcc ggataattaa gatcaaccaa
      301 cacgagaccg tgcaattctc ggcccaaggt cttgctcagc tttttaacgg cctgcactaa
      361 tccgggctca acatcgtcta ttgctaaaat gagatttttt tcttgcacca gatgagatta
      421 gcacatttat ggtcgctttc gctagacctt tttagaccgg tggatagcga tcaatagcac
      481 cgtcataaaa agaaggagca agctggctgc ggctgctact aacaccactc gatttcttct
      541 ttcgatatga ttgtgtcttg ttactgcctg tgcttttttt ggatccgccc ggaaagcatt
      601 agcagcataa ccgaaaaccg gcgaatgcgt aaaggatgcc aatacaggct cacttaaagc
      661 gatcctatct gacttacttt ccggttggat accactccga aagctatcaa ttgcaatcag
      721 ttgttgatcc tgggacttat agatatagcg gcgaatttct cctggcgcaa tggtagccaa
      781 aacacttgat tcacctgata ggtaggttga aattgccgtc tcgcccatag tatcgggatt
      841 caactcggca gcgccaactt gaaaggcgcc cgatccagtg cccttgagcg ttattgcata
      901 gtcacctact ccctgacggg gaataaaagc cagttggggt ttaaccgtgg aaccggaata
      961 ataagctccg gcgatgccga tcttcgaatc atcaataatc tgcccgctgg ggctagttac
     1021 cgtaagcgcc acaggaccgg cggcatacac gcccaaaaag cgttgtggcg gcaacaacgg
     1081 tatggtcacg tccgggacat actcccccat tatgatttgt aagatgaatg gtgcagcgtc
     1141 agatacaagt tgaagatgac cgacactaag cattaagctg cttttggcgg cggcttcgag
     1201 gcttgcttgt agaacggtac cgtcacccat cccaaacacc ggcgacacaa tgctgccatg
     1261 aggccacaca atagcactgc tatttactgc cgcaacggtt agctcccgaa gagtgctgcg
     1321 attggctgcg cctaccacat ggaacgctac tctctccttg agccgggcaa tatcagctgg
     1381 caggttgagg ctttccagaa agggatttac ggggtagcca gcaggataac ggcgcccaga
     1441 aagatagtca aataccggca gcagctgccc tatcgatgga atggcccgtc gaagcagctc
     1501 agcccgcgga acggcgatat caggattatt gagcgccgag gcaataaaat taagtaacaa
     1561 attatcggaa tccggcttta acccgctagc cacccctcct tcccaaactt catacgctgc
     1621 caccgagccg gcattcggtg tacccagttg aatcaggtta cgaatatcat gatggtacgc
     1681 agtgctttgg atgtaacggc gggccaccag gcctcccatg gaatgagcca ccacatccac
     1741 ctgggaacaa tgacattttt gccgaatatc atgcagtttc tgagacagct gcgcggccgt
     1801 ttgagcgtta tcctgcatcc agttataggg aaagacaaac aatgtggtat ccaagctgaa
     1861 gcctcggctt tgaagcgctt gaagcaaccc gtcataggta tgcctgactg gatcaagcac
     1921 tagcggggtc tgataggtgc gatcccagaa tcccgtaaaa actcgcggaa gctgagaagc
     1981 caagctttcc attgtgggaa gcgagcccag aatcccgggt atgagcagaa ccgggcgata
     2041 cacgcgcggc aggctcaagc cctggatatc gttggcccat agtccattat cgctcgctgc
     2101 ccagatgcgg ttcgcatggc gggcaacgga ggctattttg acattcaaca agctgcgggc
     2161 agcactcccg tcataggtaa ctagaaaacg gtatggaaag ccaatgctcc atgcgccata
     2221 gaaggcctga ttgccactga ctgctagccc ccttaaatgc atatcatgcc cgtagaaagt
     2281 cccgttaatg ttgccaggac cggcaagctt attccaactg gcaccgttgt cattggagcg
     2341 aaaaagaccg tcagcagaga gttgccagat taatccctct ttcacagcca ctgcagcggt
     2401 aaacagaccg gctcccctgc cgccgcccga ccaactggca gcgttgttac tcgattgata
     2461 tacccctcca gcagccccga cgtagagagt atggctagcg taatcataat caaacgccgc
     2521 aatgttgtcg ctcggcagat tcatatcctg cgcctccccg ctggcatcaa accgccacac
     2581 ccttaccggt gtgcctgctc catcatacag tgcggcatac acgtactcct cggtagtttg
     2641 gagcgccgtt acaatcttcc gccgggttat tgatgcccca aacggctgcc atgtcatgcc
     2701 gccattatca cttatataga taccgctccc ggtgccggca tacaggcggc cggcatagac
     2761 aaggtcaaaa ctaattgtgc gagccggcac agataaaagg ccgggtagcc aggtaacgcc
     2821 cgcatcatgg gaaagctgca gattgccatt tacgatagcg gccatctcat gagaactacg
     2881 ctgatttact gctaccactg ataccgatgc accggaaaag gctgcaaccc gccactctga
     2941 tgcctgtgct ggagccgccc aggcaactgg aacgataccc acaaggccgg caacaataaa
     3001 aaaggctcta tggcttaacc gctgatgaaa catgtcctac agcccctagc aaacacgctt
     3061 ccttgctccc tagcttgcca gcaatgtatt aaataatgct ttatacatca ccagaataat
     3121 gattgaaagt tcggtttttg ttcggtatat tactcacaag ggatgcttct aaaggaggtg
     3181 ttgcgatggc ggacgtaaaa tcaatcaaac ggctcagagt gtatcaaacc agccgcgaac
     3241 tggaagatag cgtgtatatg ctcgtaaaag cgctgccgga aaccgagttt tatgcccttg
     3301 gcaatgacct gcgcagaagc agtgccgcca tttcccatta ccttatgcaa tgccatcgtc
     3361 actatagcca taatctcaaa cttgaagcgc tccatcttgc acggacggaa gcagaaacct
     3421 tgcagaacct attgctcagc catgacgcca aagggtacgg ctcaaccgat catttggggg
     3481 atgcctgcat cagcgttata aagcagtgct gggggcttat aaagtacttc aagcagcgcc
     3541 aggctgaaca gagcattgcg gccagcgtta gggccagtga tgagctggta gcctcgcgca
     3601 gctagtttct gcataatgtg cgacctcctg ccattcgggt ttttgctagc ccgaaagatc
     3661 attgttgagc ggttgtcccc atttgtggta acttttccac tgcacttttt accagtcata
     3721 tagctacatg ttgtcataca tttgaagaaa agggtttaca agcaaacgtc caagcattac
     3781 tattaaaaac gtggtaagga atgctctcgg aacgatatcg cgatagcgct catcgaaccg
     3841 ggggcttttt attgaaagct accctatgaa atgcccctat tgccgagctg aatcaaccga
     3901 tgtctttaat tcccgtccca ctaagtttgg aaaccagatt tggcggcggc ggcgatgttt
     3961 gtcttgcaga gagtcattta ccacctatga gacagccgat cttggcttta taaccatctg
     4021 taaggcgtcc ggcaaacctc agccctactc gcgggccacc ctgtttgcca gtgtctttga
     4081 agcgttttta ctgactccgc agaagcagat cgaggttgat gctgtaaccg atacggttga
     4141 ggccaagttg ttagacctgc aacagccaac aattacttcg agcgaagtcg ctgaagtcgt
     4201 gctgcaaacc ttaaaacatt acaacacctc ggcatttata cgctatttat ctaatcagac
     4261 agatttggtg aatgaggccc agttacgcaa ggagctgaaa aaatattagc aacaagcgag
     4321 cgagcaccgg aaaagtgctc gctcgcttga ttttatctgt tgctatctta cccaccaagt
     4381 tgtgggtagc gcttagttct ggcttttatc tgatcggttt tctggctctt tgcgggtttg
     4441 ctcgctgtct tcgacaataa cttcctggtc ttctgctacg ccttcgggca gttcgctctc
     4501 aacagccgca tcaagttcct ctaactcctc atcactgcgc ggggccacaa ccgtcgctac
     4561 cagcgtttcg ggcggggtga ggacttctac gtttgccggg aatttcaggt cgccaatggt
     4621 aatattttta tcaaaatcat ctaagaccga gatatcaacc tcaatacttt cgggtaaatc
     4681 agctggaagg gcttccactt cgacagtttc aatcggacga gttaaggttc cttcctgctg
     4741 gtaaacagcg gtggactcac caataacgtg cagcggaatt tcagccttaa tcttttcatc
     4801 catgcgtatc aggaagaaat cggcgtggat aatgtggccg gtgcggctat cgagttgaac
     4861 atcgtgaata agggcattgt taagcttacc gtcgtccagt tttaggctaa cgatgcgatt
     4921 gccgccggct tgagcgtaca cctgttcaag ttctttggca agcacggtta cggcgatggc
     4981 cttggagcca tggccataca taattgctgg ggtattaccg gatttgcgca gattccggtt
     5041 ggttttacga accgagtcgc ggctttggag ctgaatagtg attttatcgg ccatgattct
     5101 ccttcttgat tctagcgctt cttaatcggt gacagatttc cagatgcagg cgcaactgac
     5161 actataccgc agcattacca gatgagcaag gtgctgactc ggtttgaaca ttccactacc
     5221 ggcagaaaac aacttgcttc gtaggctgcc ctgccgctcg taaaaatctt caaaccgagt
     5281 tcgctctgcg cgcgctcggg ttagcgcttc ttgctgaaac cggtacgctt gcggctaata
     5341 tagcgatgga attcggctat ttcatctgca gagatggttc ccatggaagc aaatttggcc
     5401 tcctcggctg aacgcaggtc ggtcttggag cgcttagtgg ttttaccgct ggtggcaacg
     5461 gttgccagca ccggcagtga acactcatgg cagttgagct ggacgaagca atactggttc
     5521 agctcaccaa gatagcggat atcatcaagg cagtagctcg tatcgcatga agggcactta
     5581 tagaattcct gcaggttatt aataatgtcc tgcaaatgtc ttcgactcat acgtatcccc
     5641 ttcgttacat caccattata tctggtgtcc gagcgttttg gcaatgcgtg ttgagcttac
     5701 gcggggcggc ggctgcctat ccgtatcaaa ctggcgatca gtactaccat cgtagccaat
     5761 agaacgatga gcgctacgag tttggtgaaa ctttgcgtct gcagagccac taccccaaac
     5821 gtaaccgcca gcatatagag cgataggacg gccccgcgct gcgacagacc agccttgagc
     5881 aacaaatggt gtaaatgctc ccggtcagag gtaaaagggt gcacacggcg acgcaaacgg
     5941 cgaaccaccg cccacatggc atcaataata gtgaatccaa acaccaagct aagcgttgcc
     6001 agtttccctc ccgaatagat tgccagcaac gcgagggtca gccccaggaa ataggcgccg
     6061 ctatccccca taaaaatttt agcgggaaag aaattgaatg gcagaaaccc aagcgcagac
     6121 ccagccagaa taatcgcaag caaggcaact aagggctggt tgacttccgg gctgatcgcc
     6181 agagcaaaca gaatgatcgc agcaattgct gacacgccgc cggctaaccc atcaagccca
     6241 tcgagaaagt taacggtatt caccatgccg acaatccaca gaatacttaa tgcgttggca
     6301 atgggcgtaa tgtgaaaatg aagaattccc caatcaaccg caaaacggcc ggcagtaagg
     6361 tcgataacac ctcccagggg atttgtgatg gaaacaatgc cgatgccacc cgctaaaaca
     6421 acacaggccg ccagaaactg ccaaaaaagc cggattttag cgctgagccc acgcacatca
     6481 tcaatcgccc ccaggaccgc cagcataatg cctccaataa gcagggctgc cagttggcgg
     6541 ttcattggca aaaaggttag cgcgcctatc acaaaactgg ctacaatcgc caatcctccc
     6601 aggtaaggca ctacccgatt atcaagcgca tgcgacttta tggcaaccgg ttgggcgacg
     6661 atctggtagc ggagagccag catgcggacg aacggcgtta acagcagcgc gaggccaaaa
     6721 cttactaaga atgcaacgag ataaatcatt tgcgccgaga ctttacggta acattaaagc
     6781 ccagatagcg gccaagcatc aggcgggttt gggaatcaat cgcggccagc gcgttaaaga
     6841 caatgccggt gatcggaaac aacagccact gagccaccat cgctaggctg cgccgatgac
     6901 cgtaacgctc cggccgtggg ggcagcgaaa taatactgat ggatgccatt acaaacaaac
     6961 cgatgagcgc aatactttga aggtggctgg cgattaccgg cagctcatgt gctaatccat
     7021 actggctgaa ctgaggatta agaaagagcg gcaaccaggc aacaaacgta atcaagagaa
     7081 cggccgtcgc ccaactgata tgcccctcta ataggcgtcc ggtttgaatc agtttgctgc
     7141 ctaacggtat tttattgttg cgaatgcagt tgcggacaac aaaactaaag tcggagatac
     7201 cgtaagccca ccgccgtaac tgaatatatt gagccttaaa ggttttgggg taggtttcag
     7261 ccagtacggc atcctgataa atcgggatat acaacggaac gacggcgtaa tcgccattaa
     7321 aaataaaata gctgcgccaa aactggtggc catcctccac cggcgaggta acgctccaaa
     7381 agtcggtttc aatcaaggcg cgcaacggtt gggcatgcga cgaaaagttg cgcagccgat
     7441 gcgggcgcat catctccatg atctgataaa acgaattgcc agcggcaatc acccgcatcg
     7501 gcgccggcgc atcccatata ttgttcaaaa acatcggtat cggctggtat gatttatgaa
     7561 cgcggttagg atccaatgca tatacatagg taaggtaggc aaaatactgg ggatccggct
     7621 tgttgtcagc atcaagcgtt gtcaccacca cgtgttcggg atctatcccc cgctcaataa
     7681 tgtccgccgt tgcctggcgg gccgcatagg taatgttcgc tcctttgccc cgcaattctc
     7741 ccggccggtt atcggggtgc ataatagccg atgcgtagtt gaaacgcttg ccatagcttg
     7801 caatcaaatc ttcagcagtc tccttcaccg cctgcccgcc acgttcttcg taagcgataa
     7861 ccacaatcat ccgctcggac ggatagtcag cctccaagag cgatttcaca gtcggctcga
     7921 gcacgtcacg cgattcatta taggtcgcaa taataaccag atgacaaatg ctgctgggcg
     7981 gcataatagc ggtttggtgt gattcaatct cttttaatga ggatagatcg gacaatgcct
     8041 gcacatagcc gcgacgctgc ttagtgtgac ttcgattcca aaagaagcgc cgctgaacct
     8101 tagggtagcg ccgcagaagg tcttgaatgc gacggtcgat ggctttaata tgcttttccg
     8161 gatcttccaa ccagttcaag cgctcccgcc agttcaaggc ttgatggtcg cgcagacgcc
     8221 gatacccgcg cactaaatag gtgctcatcc gcaaagcctt gagcagccag aacaagtcga
     8281 atgcgatgat gaaatacgac acgtaaatcg gcaagaagaa gcttaaaata atcgggccga
     8341 taagcagggt ccaggtaatg gctccgggta taatttccaa catccggata aaccagcgtt
     8401 ggtccgtagc gtagcggtat ttagttcgaa cggcttcggt catataatcg cggcaaactc
     8461 cgagccaatt accagacaca gcaaaccgac cgcaaccgcc ccaataagca agaaaaagct
     8521 ggttaagcgg ttacgctgga agctctgtcc ggctaagagc gcattaataa tggttaccgc
     8581 aaaaccaaag gctgcaatcc ggtagttttg ataccagggc cccacttgat caaacccgat
     8641 taagctcaaa taatgaaccg gtaccggata atcgacttga tgtaccttca ccagcataac
     8701 gcccagcgtt aaaatattca gtaccaggct gatccccagc gccgtcagag aaaccggatc
     8761 cttcatcatg ggatgttggc gcagatcact aaggttaagg cgtcggagca cctgcagcgg
     8821 tgcattcaaa ttcatggggc cattatagca ctttttatct gggaaagggc ctatggttgg
     8881 aactcaggcg ctcaggaaat gtactcaggc gctcttttct cttaatgcac atttgtaaca
     8941 taatggaaga aatgtgcatt aagagaaaag agcattggag ccatcacccg ggcagtcagc
     9001 catcagctag ccagagaagg tgatttttga tttaatacca gggtccctat gccgatgtag
     9061 ctcagttggt agagcaatgc tttcgtaaag cagaggtcgg cggttcaaat ccgcccatcg
     9121 gctccattcg gtacagtaga agcatgcggg tatagttcat cggtagaatg cgaccttccc
     9181 aaggttgaga ggcgagttcg attctcgcta cccgctccaa ttacatttca ttttctcctc
     9241 caccataatc tttccaattt catcaatggc tgcgggacac caggagtctt tagtaaatgt
     9301 agtaaattgt tatgaagcgg ctttttgcag aacttggcgc gacagaacgc gcggggccag
     9361 gagtaaatac gcaagggcca caaccacaac caccccgcca aaaactttaa tggccgtaaa
     9421 gttgttcgag taat
//
LOCUS       DASVGB010000025         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_543292, whole genome shotgun sequence.
ACCESSION   DASVGB010000025 DASVGB010000000
VERSION     DASVGB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543292"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /gene="tsaE"
                     /locus_tag="VF272_00720"
     CDS             complement(<1..387)
                     /gene="tsaE"
                     /locus_tag="VF272_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7259437.1"
                     /translation="MIELKGDVGSGKTTLTQGIAAGLGFSGEVSSPTFTLSRAYPLKD
                     GSSLHHFDFYRLKGHDIATEELYEVTQDPTAIVVIEWPGQGAAKLPARRLRITIAPLE
                     QENHRSILLESLAGGGEAVIGKISHDT"
     gene            complement(380..1741)
                     /locus_tag="VF272_00725"
     CDS             complement(380..1741)
                     /locus_tag="VF272_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7259438.1"
                     /translation="MASPSRASANLIPHLVISEVQTGTTTSASQEFIELYNPLPEPVS
                     LTGWTIEYKAATSVNAETSWSKKATLLGMVPAQGFYLVAPKAYLVLADAEWTATLASG
                     GGTIRLKDAAGVVVDQLGYGETANAAEGLPAPAPAAASIERLPGRTQPLEGNGVDTND
                     NAADFILRTEAQPQAATSLPEVPGEIQFEEAEEPDEPTELAVTAPMYSALIITELLVD
                     PLAPLSDSHDEFIELYNPNAEPVDMEGYMLRCGSSFRDYYKLSKMIIPPGGYAVLYSR
                     ETGLSLTNSGGAVQLLDPNGTLVDQTPVYSAASAGQSWAAFEDKWEWTLQLTPGLSNV
                     LALLPATTGTTALAKAKTAPKKAAVKPAAKPKKAAVSKLAKEKKPKKIKPTPAAFTPL
                     PVAASRNLQPGTWLIISLAVLTIGYALYEFRYDIFNLYYRLRGKHSSRSDNRHPVKGR
                     GRD"
     gene            1950..>2529
                     /locus_tag="VF272_00730"
     CDS             1950..>2529
                     /locus_tag="VF272_00730"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEX7259439.1"
                     /translation="MRVSDSELKSLLLASKKVKPDMLQAAEEAREQTGEPLLQTVLKR
                     KLITEQNLLKLYAKSVDVPYVDLRNAEIPHDILTKIPERIARKYQAVLFGQDGTTLQL
                     AMADPEDFQAADFIAKQIGQDITIYVASSSDIMAAIDQYKGNISTEITKAIKDSSTDE
                     NDKQQEVSAKDLAEDAPIAKTVNIVLEYAVKAR"
BASE COUNT          616 a          669 c          635 g          609 t
ORIGIN      
        1 agtatcatgg ctgatttttc caattactgc ctcaccgccc ccagcaagac tttccagcaa
       61 gatactacgg tgattttcct gctccagcgg ggcaatcgta atacgcagtc ttctagccgg
      121 cagtttagcc gctccctgcc cgggccattc aatcaccacg atagcggtgg gatcctgcgt
      181 aacctcatac aattcttcag tggcaatatc gtgccccttc agtctataaa aatcaaagtg
      241 atgaaggctg ctgccatctt ttaggggata tgcccgactc aaggtaaagg tagggctgga
      301 gacctcgccg ctaaagccaa gtcccgcagc aattccttgt gttagagtag tctttccgct
      361 cccgacgtcg cccttcaact caatcacgtc ccctcccttt aactgggtgc cgattatctg
      421 accgagacga atgcttgccc cggagccggt agtagaggtt gaatatgtcg tagcggaatt
      481 catagagagc ataccctata gtaaggactg ccaggctgat aatcaaccag gtgccgggct
      541 ggagattgcg agaggcggct actggcagag gcgtaaatgc agcgggagtc ggtttaattt
      601 tcttgggctt cttctccttc gccaacttcg ataccgccgc ttttttcggt ttagccgccg
      661 gcttaaccgc cgccttcttc ggggccgttt tagccttagc tagtgccgtc gttccagttg
      721 tcgctggcaa aagcgccagc acattggaaa gtccgggagt tagctgcaac gtccactccc
      781 atttgtcttc aaaagccgcc caagactgcc cggcggatgc ggctgaatac acaggagttt
      841 ggtcaaccag cgtaccgttt ggatcaagca actggaccgc tccgccactg tttgtcaggc
      901 tgagaccagt ctcgcgcgaa tacaggacag catatccccc gggaggaatg atcatcttgg
      961 atagcttgta gtaatcccga aagctgctgc cgcaacgcag catatatcct tccatgtcaa
     1021 ctggttcggc gttcggatta tacagctcaa tgaactcatc atggctgtca cttagcggtg
     1081 ccaaaggatc caccaatagc tcggttataa taagggctga atacattgga gccgtgacag
     1141 ccagttcggt tggttcgtct ggttcttctg cttcttcaaa ttggatttct cccggtactt
     1201 ccggaagaga agtggctgct tgcggctgag cctcggttcg gagtatgaaa tcggcagcgt
     1261 tatcgtttgt atctacccca tttccctcaa gcggctgggt acggccaggc aggcgctcaa
     1321 tactcgcggc ggcgggtgcc ggagccggca atccttcggc tgcattggct gtttcaccgt
     1381 atcctagctg atcaacaacc acgccagcag catctttgag gcgaatagtt ccgccaccag
     1441 atgctaacgt tgcagtccac tcagcatcgg ctagtacaag ataagccttt ggggcaacta
     1501 ggtagaatcc ctgggccgga accataccaa gcaaagtggc ttttttagac caactggttt
     1561 cggcattaac cgaagttgcc gccttatact caatagtcca accggtaagc gatacgggct
     1621 ccggcaaagg gttgtagagc tcaatgaact cttgactggc actggttgtc gttccggtct
     1681 gaacttcgct aattacgagg tggggtatca aatttgcgct ggctcgggaa ggtgatgcca
     1741 ttagtggtaa caaagccagc gccaaagtaa tacggactat agaatacata gttgtctccg
     1801 tttaatgctg tgataacggt atttctaggt gtcagagcta tcacattcac tagtttgcca
     1861 ggctggcatt aaattgttct taccgcgttc ttacgccagg attaaacttc atctggaatt
     1921 tatcccgctt atgctacaat ataggtgtca tgcgtgtatc tgatagcgag ttaaaaagtc
     1981 tgttgcttgc gagcaagaag gtaaagcccg acatgctgca ggcggccgaa gaggcgcgcg
     2041 agcaaaccgg cgagccgctg cttcaaactg tgctcaaacg caaacttatt accgagcaga
     2101 acttgctcaa actgtacgcc aaaagtgtag atgttcccta tgtggattta cgcaatgccg
     2161 aaattccgca tgacatactg acaaaaatcc ccgagcgcat cgcccgtaaa taccaggcgg
     2221 tattattcgg ccaggatggc accaccttgc agctagcgat ggccgatccg gaggatttcc
     2281 aagccgccga cttcatcgcc aagcaaattg gccaagatat caccatttat gtagcctcat
     2341 ccagcgacat tatggccgcc attgatcaat acaagggaaa tatttccacc gagattacca
     2401 aagccattaa agacagtagc acggacgaga atgacaagca gcaagaagtc agcgccaaag
     2461 atctggctga agacgcgcca atcgctaaaa ccgttaacat cgtcctggaa tatgccgtca
     2521 aggccaggg
//
LOCUS       DASVGB010000026         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_540732, whole genome shotgun sequence.
ACCESSION   DASVGB010000026 DASVGB010000000
VERSION     DASVGB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540732"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /gene="ahcY"
                     /locus_tag="VF272_00735"
     CDS             <1..1092
                     /gene="ahcY"
                     /locus_tag="VF272_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017855632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7259440.1"
                     /translation="LGAEIRWASCNIFSTQDQAAAAIAKTGVAVFAWKGETEDEYWWC
                     IDQTLNFPHGKGPNLLLDDGGDLTARVHDKFPELLKDIKGVSEETTIGVHHLHQMLES
                     GRLKLPAINVNDSVTKSKFDNKYGCRESLVDGIKRATDIMLAGKVAVVAGYGDVGKGS
                     AQSLASYGCRVLVTEIDPICALQAMMEGYEVITMEDAAKEGDIFVTTTGCEDVITLDH
                     MKVMKDTAIVCNIGHFDVEIQVDSLNKLPGIKKQEIKPQVDVYTFPDGHRIILLAEGR
                     LVNLACATGHPSFVMSNSFTNQVLAQIELYTKHYTVGVYVLPKELDEKVALLHLEALG
                     GKLTKLSPGQAKYLGLPKNGPFKPEHYRY"
     gene            1236..3008
                     /locus_tag="VF272_00740"
     CDS             1236..3008
                     /locus_tag="VF272_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7259441.1"
                     /translation="MGEAIKPEVGATVHKDGVTFRVWAPFASSVAVTGSFNDWSPNAD
                     PLTSENDGYWATTVGHAQIGQEYNFIIRNGDRELQRNDPRALQLTASGDASLIIDPNF
                     DWEDDNFTIAPVNEQIVYELHIGTFHRTDPATPGTFETAAAKLDYLADLGINVIELMP
                     VSNTTIDRWWGYDPDHLYAIDAAYGGRQAFLEFVKAAHKRGIGVVIDVVYNHLSPYPG
                     MDLWQFDGWSENDKGGIYFYNDWRGDTPWGPRLDYGRPEVRQYITDNVRMWLRDCHVD
                     GLRVDAVIAIRNGVGRNEDPSTDLPDGWRVLQELNTTAFEVKSEAMMIGEDIASNAWV
                     TKPVGEGGAGFMAQWETSFPSILRNALNPDHDSDRHLQPIQDVLSKNYNGDIFQRVIY
                     SESHDAAANGHQRLNEEISPGAADNLFARRRSALAAALVLTAPGIPMFFQGQEFMETG
                     WFNHWKSLDWEKTEKFQGILQLYKDLIALRRNHDQRTSGLMGRGFDTQHFDEQSKVLA
                     FHRWDQGGPRDDVIIAINFSNQQQDNYEIKFPQKGLWKVRFNSDWQGYSADFGNTTTP
                     DIAVENASGAVNLGPYSVIILSQD"
BASE COUNT          820 a          754 c          797 g          754 t
ORIGIN      
        1 ctgggagctg agatacgctg ggcttcctgc aatattttca gcacccaaga tcaagccgca
       61 gcggcaatcg ctaaaaccgg cgtggctgtc tttgcctgga agggcgaaac tgaagatgaa
      121 tactggtggt gcatcgatca aactttgaat tttccccatg ggaaagggcc taatttgctc
      181 cttgatgacg gcggtgattt gacagctcgg gtgcatgata aattcccgga attgctcaaa
      241 gacatcaagg gtgtatccga agaaactact atcggtgttc accacctcca tcaaatgctt
      301 gaaagtggaa gacttaaact cccggcgatt aatgtgaatg acagtgttac caagagcaag
      361 tttgacaaca agtacggttg ccgcgagtcg ctcgttgacg gcattaagcg ggccaccgat
      421 atcatgctgg cagggaaggt tgcggtggtt gccggatatg gcgacgtcgg taagggttcc
      481 gcccagtcct tagcttccta tggctgccga gtccttgtaa ccgaaattga ccctatctgt
      541 gctcttcagg ccatgatgga aggatacgaa gtgataacca tggaagatgc ggccaaggaa
      601 ggagatatct ttgtaaccac aaccggctgc gaagatgtta ttacgcttga ccacatgaaa
      661 gtaatgaagg acaccgcgat tgtctgcaat attggccact tcgatgtaga aattcaggtt
      721 gattccttga ataaattgcc tggtattaaa aagcaggaga tcaaacccca agttgatgta
      781 tatacttttc ccgacggaca ccggatcatc ctccttgccg aaggacgatt ggtaaacctg
      841 gcctgtgcca ccggacatcc ctcatttgtg atgagcaatt cctttaccaa ccaggtattg
      901 gcgcaaattg aactgtatac caagcactat acggtcggag tttatgtgct gccaaaagag
      961 ttggatgaaa aagtcgccct tttgcacctt gaagcgcttg gcggcaaact gaccaaacta
     1021 agtcctggcc aggcaaagta tttgggtctg ccgaaaaacg gaccatttaa gcctgaacac
     1081 taccgctact agccggaggc cagttagaac aattcagtcc ctacagagag ctccttgcct
     1141 cgcaagcggc acagcagtcg taaaatcatt tctggccatg ctattgcaat gcaaggcgag
     1201 cataaggata atgataagtg atatagggtt agtgcatggg tgaagcaatc aaacctgagg
     1261 tgggagcaac cgtccataag gacggggtga catttcgcgt atgggctccc tttgcttcga
     1321 gtgtagccgt aaccggtagc ttcaatgatt ggtcgccaaa tgcagatccg ttaacgtcgg
     1381 aaaatgacgg ttactgggcg acaacagttg gtcacgccca gattggtcaa gaatataact
     1441 ttattatccg taatggtgac cgggaattac aacgtaatga cccgcgagcc ctccaactga
     1501 ccgcttctgg cgatgcgtca ttgattattg atccgaattt tgattgggaa gatgacaatt
     1561 ttaccatcgc gccggtaaac gaacagattg tgtatgagtt gcacattggc acctttcatc
     1621 ggaccgaccc ggctaccccg ggcacctttg agaccgctgc cgctaaactc gactacttgg
     1681 cagacctcgg cattaacgta atcgaattga tgccggtcag caatactacc attgatcgct
     1741 ggtggggcta cgatcctgac cacctctacg ccatcgacgc tgcctatggt ggtcggcagg
     1801 cttttctgga attcgtaaag gccgcccaca aaaggggtat aggcgtcgtc attgatgtcg
     1861 tctacaacca cttaagccct tatcccggta tggatttatg gcagtttgac ggctggagtg
     1921 aaaacgataa gggtggcatt tatttctata acgactggcg gggtgacacc ccctgggggc
     1981 cccggcttga ttatggacgt ccggaggttc gccagtatat taccgacaat gttcggatgt
     2041 ggctccgcga ctgccatgtt gatggcttgc gcgtggatgc ggttattgca attcgcaacg
     2101 gagtcggccg caatgaagat ccatccaccg accttcccga tggatggcga gtgctgcagg
     2161 aactcaatac aacagccttc gaggtaaaat ctgaagccat gatgattggc gaagacattg
     2221 cctcaaatgc ctgggtaacg aagccggttg gagaaggcgg agccggattc atggcacaat
     2281 gggaaacatc ttttcccagc atcctgcgca atgcccttaa tcccgaccat gacagcgaca
     2341 ggcatctgca gccaattcaa gatgttctat caaagaatta caacggcgat atatttcaac
     2401 gggtaattta tagcgaatct catgatgctg ccgccaacgg ccaccaacgt ttaaatgaag
     2461 agatatcccc tggtgccgca gataatttgt ttgcccgccg gcgctcagcc ctagccgccg
     2521 cgttagtttt aaccgcacca ggcattccta tgttctttca aggccaggag ttcatggaaa
     2581 ccggttggtt caaccactgg aaatcacttg attgggagaa aacagaaaaa tttcagggca
     2641 tacttcagct ttataaagat ttaattgcct tgcggcgcaa tcatgatcaa cgaacctccg
     2701 gacttatggg gcggggcttt gatactcagc atttcgacga gcagtccaag gttctggcat
     2761 tccatcgctg ggatcaaggc gggccacggg atgatgtgat tatcgcgatt aatttttcca
     2821 atcagcagca ggataactac gagataaaat ttccccaaaa aggactttgg aaggtacgct
     2881 ttaatagtga ttggcagggg tacagtgccg acttcggcaa taccaccaca ccggacattg
     2941 cagtagaaaa cgctagcgga gcggttaacc ttggtcccta ctcggtcatt attttgtcgc
     3001 aagactaaac atgcctttac tctatactta cctatatgag taaaactgat cttccggcca
     3061 cgcttagcgg catggtcacc ccattcaagg ggaatggtcg aaaactggga tacccgaccg
     3121 ctaat
//
LOCUS       DASVGB010000027         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_824433, whole genome shotgun sequence.
ACCESSION   DASVGB010000027 DASVGB010000000
VERSION     DASVGB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824433"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /gene="ftsH"
                     /locus_tag="VF272_00745"
     CDS             <1..1559
                     /gene="ftsH"
                     /locus_tag="VF272_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7259442.1"
                     /translation="SILPFVLIGAFLFFMMRQAQGSSNQAMSFGKSRARLFGSDKKKV
                     LFKDVAGSEEAKQELSEVVEFLKYPTKFEALGAKIPKGVLLFGPPGTGKTLLARAVAG
                     EAEVPFFSISGSEFVEMFVGVGASRVRDLFAKAKKNAPCIIFVDEIDAVGRQRGTGLG
                     GGHDEREQTLNQILVEMDGFEQGTNVIVMAATNRPDVLDPALLRPGRFDRRVTLDAPD
                     VHNRKAILDVHALGKPLDPATNLMQIAKKTPGFSGADLANLLNEAAILTARHNRKKIT
                     QHDLDMSVEKVLLGPERRSHIMNAREKEITAYHEAGHAIVGHYMENAHPVHKITIVPR
                     GNAGGFTWSLPVEDRHLSSVQDFKDDMAMMLGGRLAERIVYDEITTGASNDLQNATNL
                     ARKMIMDYGMSDKLPNLVFGRQSDAIFMGRDLGEGKNYSDDIAKVIDDEVAVLIDEAA
                     KRAHDTITKHRKELDAVADFLVKDETMEEDEFLKIVGGPNNKKKYKAPAIDIGGDTGT
                     AKPAGKLAEA"
     gene            1649..2485
                     /locus_tag="VF272_00750"
     CDS             1649..2485
                     /locus_tag="VF272_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259443.1"
                     /translation="MNAQPNRAINATYCMRLLIIIFLGLLPVLAFSASSISNGFFSKT
                     ALSPGTIVSQGSSTEVVIPAHASSITTLLGVVTTNSEIAFTSDADKVQVVSGGIAETF
                     VSDAKGDIKAGDKITVGELSGVGVRLSGSGKTVGTAQANFNRKSAGAKTQNVADVNGK
                     THRIAIGQIPVAVDVADYVAPSKKDTLVPAIVQLFFNHLMGRDVSGRAILVATVLLLA
                     GVIVAGIILYGAVRGGMVSLGRNPLSQRGILRAFGQAALVGLGALMVCAGLAYVAVLV
                     IN"
     gene            complement(2516..>3391)
                     /locus_tag="VF272_00755"
     CDS             complement(2516..>3391)
                     /locus_tag="VF272_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259444.1"
                     /translation="TASRKKIGVVTTRRLSIAAGLIAAALPAIGLALFLGFGAPSKTT
                     TDSVQLQDITSDELKQFTNAFLKPTTNQVVTITPNAHFNNAVTVEKTLSAGAISTPNL
                     NVTGPVNLDSVNINSNVFVNGGTTLQGVVEARNQVNVRGSLTAANASFSGNLAVAGTL
                     TAGALSVGELTTGNLIANGNLTVNGHLSLMGGAVTATAGPGAGGGSVQVFGNDVTGTV
                     VITVGGGPVAGQMAIINFKKAYSGVPHVLLTPIGQNAGSLQWYATRAASFFSIDTASA
                     PAAGTEYAFDYFVQK"
BASE COUNT          827 a          781 c          931 g          852 t
ORIGIN      
        1 ttagtattct gccctttgtg ttgattgggg cgttcttgtt ctttatgatg cgccaggccc
       61 agggttcaag caatcaggca atgagtttcg gcaagagccg ggcacgttta tttggcagcg
      121 acaagaagaa ggtactgttt aaagatgttg ccggttcgga agaggccaaa caagagctaa
      181 gcgaggtggt tgagttcttg aaatacccaa ccaagtttga agccttaggc gctaagatcc
      241 ctaagggcgt acttttattt ggtcctcccg gaactggcaa aaccttgctt gcgcgagctg
      301 tagccgggga ggccgaggtg ccattcttta gtatttccgg ttcggaattt gtcgaaatgt
      361 tcgttggtgt cggcgcctcc cgtgtccgag acttgtttgc caaggctaaa aagaatgccc
      421 cttgtattat tttcgtcgac gaaattgatg ctgttggacg ccagcgcggt actggcttgg
      481 gcggcggcca tgatgaacgc gagcaaacct tgaaccagat tttggttgaa atggacggct
      541 ttgaacaggg caccaatgtg atcgtaatgg ctgctactaa ccgacccgac gtgcttgatc
      601 cggcattact tcgtcccggc cgctttgacc ggcgagttac cttggatgct cccgatgtac
      661 acaaccgcaa ggcaatcctc gacgtacacg cattgggcaa gccgcttgat ccggctacca
      721 acctcatgca gattgctaaa aaaactccag gcttttccgg tgctgatctt gctaacttat
      781 tgaatgaagc ggctatttta actgctcggc acaaccgtaa aaaaattact cagcatgatt
      841 tggatatgtc ggttgaaaag gtattgctag gacctgaacg ccgcagccat attatgaacg
      901 cccgcgaaaa agagattacc gcctaccatg aggccggtca tgcgattgtc ggccattaca
      961 tggaaaatgc ccatccagtc cacaagataa cgattgtccc acgcggtaat gctggcggat
     1021 ttacctggag cctcccggtt gaagaccggc acctttcaag tgttcaagac tttaaagacg
     1081 acatggcgat gatgctaggc ggccggctgg ctgagcggat tgtctatgac gaaattacga
     1141 ccggtgcatc gaatgactta cagaatgcca ccaatttggc tcgcaagatg attatggatt
     1201 acggtatgag cgataagctg ccgaacttgg tattcgggcg gcagagcgat gcaatattta
     1261 tggggcgcga cctcggggaa ggtaaaaatt actctgacga tatcgctaaa gtcatcgatg
     1321 atgaagtagc agtgctcatt gacgaagctg ctaagcgggc acatgacacc atcacgaagc
     1381 accgtaaaga actcgatgcc gtggctgatt ttctggtgaa agatgaaacg atggaagaag
     1441 atgagttcct gaagatcgtt ggcgggccga acaacaaaaa gaaatataag gcaccggcga
     1501 tagatattgg cggcgacacc ggtacggcca aaccggcggg aaaactggcc gaagcttagg
     1561 atctaacgaa gcgagtaaac tgtcggttag ataagcataa gtgctataat tatgggaaag
     1621 ttttaagcac ttgtgaaggc agaaacttat gaacgcacaa cccaatcgtg caattaacgc
     1681 cacttactgt atgcggttgc tcattattat ctttcttggg cttttgccgg tgttggcatt
     1741 ttctgcctcg tcgatatcaa atggcttctt ttctaaaacc gctttgtcgc ccggcacaat
     1801 tgttagccaa ggcagtagca ccgaagttgt gattccggct catgcctcga gcattactac
     1861 tctattaggc gttgttacga ctaattcaga aatagctttt acatccgatg ctgataaagt
     1921 tcaggtggta agcggaggta ttgcggagac tttcgtatcc gatgctaagg gtgacatcaa
     1981 ggctggtgac aaaattacgg ttggcgagtt gagcggcgtg ggggtgaggc tgtctgggag
     2041 tggtaaaaca gtaggaaccg ctcaggccaa ctttaatcgc aaaagtgctg gggctaaaac
     2101 ccaaaatgta gccgatgtta acggaaagac acatcgaatt gccattgggc aaataccggt
     2161 tgcggttgat gttgcagatt acgtcgcgcc gtccaaaaaa gatacgttag tgccggcgat
     2221 agtgcagctg ttttttaacc atttaatggg gcgcgatgtg agcggtcggg cgattcttgt
     2281 ggccacggtc ttgttgctcg cgggggtgat tgttgcgggt attattcttt acggtgccgt
     2341 acgtggggga atggtatcac ttggtcgtaa tccgttgtct cagcgcggta ttcttagagc
     2401 ttttgggcag gcagcactcg ttgggcttgg tgcccttatg gtgtgcgccg gcctcgcgta
     2461 cgtagcggta ttggtgatta attaaattac tcctgcggat tcctgacgca agcgactatt
     2521 tttgaacaaa atagtcaaac gcatattcgg taccggctgc tggggccgag gcggtatcaa
     2581 tagagaagaa actagcagcg cgggtagcat accattgcaa acttccggcg ttctggccaa
     2641 tcggcgtaag caagacatgc ggtacaccgg agtatgcttt cttgaaattg atgatcgcca
     2701 tctggccggc cactggccct cctccaacgg tgatcacgac ggtgccggtt acatcgttgc
     2761 caaacacctg gacactgcca cccccggccc ccggcccggc tgtggccgta accgcgcctc
     2821 ccatgagtga taggtggcca ttaaccgtta ggttgccgtt ggcaattaag tttccggtag
     2881 ttagctcgcc tacggaaagc gcaccggcgg taagtgtgcc agccacagcc agattaccgc
     2941 tgaaactggc attggcagcg gttagcgatc cgcgaacgtt tacctgattg cgggcctcta
     3001 ccaccccttg aagcgtggtt ccgccattaa caaatacgtt tgaattgata ttcacactat
     3061 ctaagttcac cggtccggta acgttaaggt tgggcgttga aatcgcaccg gccgacaatg
     3121 ttttttcaac ggtaaccgca ttgttgaagt gagcatttgg cgtaattgtc actacttggt
     3181 tggttgttgg tttcaaaaag gcattcgtga attgtttaag ctcgtcggat gtgatgtcct
     3241 gtaattgaac agaatcggta gtagtcttgg acggcgcgcc aaatccaaga aataaggcca
     3301 acccaatagc tggaagggcg gctgctatca ggcctgccgc aatcgatagc cggcgggttg
     3361 ttacaacacc aatctttttt cgcgatgcag t
//
LOCUS       DASVGB010000028         2772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_823676, whole genome shotgun sequence.
ACCESSION   DASVGB010000028 DASVGB010000000
VERSION     DASVGB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823676"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..890
                     /locus_tag="VF272_00760"
     CDS             102..890
                     /locus_tag="VF272_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX7259445.1"
                     /translation="MLIYGKPVVESLREEQARRIKEAGSSPKLVIVQSGNNLASSTYI
                     RMKKAYGESIGAVVDHIQTGSTTAELLPLIDRLNKDGSVNGIIVQLPLPDITITDEVV
                     GSIALEKDVDGLTGKSEFYSATAMAVINLLDFYGIELKGTKVAMVGYGRLVGKPLTAL
                     LKERQAEVTVCDVKTPDTGAVTRASKVIVSATGRAGLIKPGMVTDGTFIVDVGTAEDK
                     GSIVGDVDPELLENDTLKITPARGGIGPITVSMLFEQLLKAVKI"
     gene            complement(880..1446)
                     /locus_tag="VF272_00765"
     CDS             complement(880..1446)
                     /locus_tag="VF272_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7259446.1"
                     /translation="MIHAIIFDCFGVLYNDPSLDVIRPFVPEPTPEYYRLSRAFDLGE
                     MTKEEFYAGLANITHQSTQDVAEHMEDTSGLNRELVERIRTHKPNYKIGMLSNISTAF
                     LQQFLDNHNLRDLFDVIITSQEEGFMKPQPEIFNLTLGRLGVQPDEAIFIDDREDNIR
                     GAEAVGITSILYKNNAQLNQELEAKLKS"
     gene            complement(1476..1922)
                     /locus_tag="VF272_00770"
     CDS             complement(1476..1922)
                     /locus_tag="VF272_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7259447.1"
                     /translation="MRENSEPGEILPEHEKAAKYGYEMADISEASGLPSDDDVETEVI
                     ERVGAQQAVPEALKTISPRQREILEAEAGVHGPSVIHKEIGEKLGVTKQRVHFVATEA
                     KKKMAAQPGLDVWRDESHAKEALAESDKSFLDTSNPDEESRSDMQD"
     gene            complement(1947..2023)
                     /locus_tag="VF272_00775"
     tRNA            complement(1947..2023)
                     /locus_tag="VF272_00775"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1987..1989),aa:Val,seq:gac)
     gene            2323..>2772
                     /locus_tag="VF272_00780"
     CDS             2323..>2772
                     /locus_tag="VF272_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259448.1"
                     /translation="MEADNRMLTQEIAEAIHAKVGTLVNQPLSLVDSGGQILVSNNPQ
                     GGSSLIIEDHPNAISISHNSQVAGYVVLAREVPNYEEIAPLIRSIAELMMHQALLIQQ
                     LPHQEERLDKFVYDLLTSNNVDQPTLMAEARLFNLDLSQPRICLVIYV"
BASE COUNT          719 a          700 c          655 g          698 t
ORIGIN      
        1 tcaagcgctc taaccaactg agctacagat ccttgtaaaa gagagctagc gatacgaacg
       61 tattgtacta tactggatat attcttaagc tttgaggtta tatgctgatc tacggaaaac
      121 cggtcgtcga aagcctgcgc gaagaacaag ccaggcgcat aaaagaggct ggcagcagcc
      181 caaagctcgt tatcgtccaa tccggtaata acctcgccag cagtacctac attcgcatga
      241 aaaaagccta tggcgagtcg atcggcgccg tggtggacca cattcaaacc ggcagcacca
      301 cggctgaact gttgccccta attgataggc tcaacaagga tggcagtgtt aacgggatca
      361 tcgtccagct gccgcttccc gacattacga ttaccgatga agtcgtaggc agcattgccc
      421 tagaaaagga tgtggacgga ctaaccggaa aaagcgagtt ttattcggcg accgccatgg
      481 ccgtcatcaa cctcctggat ttttatggca tagagcttaa ggggacaaag gtcgccatgg
      541 ttggctacgg ccgcttagtc ggcaagcccc tgactgccct actgaaagaa cggcaggctg
      601 aggtaacggt gtgtgatgtt aagactcccg acaccggcgc tgttacccgc gcatctaaag
      661 tgattgtcag cgccaccggc cgggccggct tgataaagcc cggtatggtt accgacggta
      721 ctttcattgt ggacgtcgga actgcggagg ataaaggatc aatcgtcggt gatgttgatc
      781 ccgagctctt ggaaaacgac accttaaaga ttacgccggc ccgcggcggc attggaccga
      841 ttacggtttc gatgttgttt gaacagcttt taaaggccgt taagatttaa gcttggcttc
      901 gagttcctga tttaactgag cattattttt atagagaata ctggtaatgc caacggcttc
      961 agcgccgcga atgttgtctt ccctgtcgtc aataaagatg gcctcatccg gctgaacgcc
     1021 taggcgccca agggtgagat taaatatttc cggctgcggc ttcataaaac cctcttcctg
     1081 ggatgttatg atgacatcga agaggtcgcg caaattatgg ttatctaaaa actgctgcaa
     1141 aaaggcagtg ctaatattcg acaacatacc tatcttataa ttcggcttat gcgtacgtat
     1201 acgctccacc agctcccgat tcagcccgga agtatcctcc atatgctctg ccacgtcctg
     1261 cgtgctctga tgcgtaatat tggccagccc agcatagaac tcttccttgg tcatttcgcc
     1321 aagatcaaaa gcccgtgaca gccggtaata ctccggggta ggctcaggca caaaggggcg
     1381 gataacatcc aatgacggat cgttatagag cacgccgaag caatcaaaaa taatggcatg
     1441 tatcatactt gcataatagc agatggtgca aaaagctagt cctgcatatc tgaacgggac
     1501 tcttcgtctg gatttgaggt atcgagaaag cttttatcac tttcagccaa tgcttctttt
     1561 gcatggcttt catcgcgcca cacgtctagt cccggctgcg ctgccatttt tttcttagct
     1621 tccgtggcga cgaaatgcac ccgctgttta gtaacgccta gcttttcacc aatttctttg
     1681 tgtatcaccg aaggcccatg aactccagcc tctgcttcta gtatctctct ttgacgaggc
     1741 gaaattgttt tcagtgcttc cggcacagct tgctgcgcac caacccgctc gataacctct
     1801 gtctccacat cgtcgtcaga gggcaaaccg gaggcctcag agatgtccgc catctcgtaa
     1861 ccatacttgg cagccttctc atgttcgggg agaatttctc ccggttccga attttctctc
     1921 atcgtaaacc aatccctatt agtgcatggt gggctctaca ggactcgaac ctgtgacctc
     1981 ttccacgtca aggaagcgct ctagccaact gagctaagag cccgtaccta agtgaatata
     2041 gcgaacccat attataaaag caattattct gcacctccca cattaagtaa atggcaaata
     2101 ctcaaaattg agtacggatg tactcaaaaa gtactctgca ttgtgtatat tttgagtaca
     2161 tccgtacaca catctgagta tgccgcgcaa aataatgttt taggtggcag cgtatcaaga
     2221 agcttatgtt atgcaattat ccaaaaatga atttggttac gcttgctgcc gatgagtctt
     2281 acagagtaag ctgataggac aagattacaa aaggtggagt gtttggaagc cgataatcgt
     2341 atgctgactc aagaaatcgc ggaagcaatt catgctaaag tcgggacgct cgttaaccag
     2401 cccctatccc tggtcgacag cggcggacag attcttgtga gcaataatcc gcaaggcggc
     2461 agcagcctca ttattgaaga ccatccaaat gcgattagca tatctcataa tagccaagtt
     2521 gccggttatg ttgtcctggc tcgcgaggtc ccgaactatg aggaaatcgc gccgctcatc
     2581 cgttcgattg ctgagcttat gatgcaccag gctttgctga ttcaacagct tcctcaccag
     2641 gaagaacggc tcgataaatt tgtctatgac ctcctaacga gtaacaatgt ggaccagccc
     2701 acgctcatgg ctgaagcccg gttgtttaat ctcgatttaa gccaaccgcg tatctgcctg
     2761 gtaatctacg tt
//
LOCUS       DASVGB010000029         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_107837, whole genome shotgun sequence.
ACCESSION   DASVGB010000029 DASVGB010000000
VERSION     DASVGB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107837"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VF272_00785"
     CDS             complement(<1..927)
                     /locus_tag="VF272_00785"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7259449.1"
                     /translation="MIKGLTKLQKLSFVISGLVISGLAFILILYIFKPPSNSAKKPAA
                     SPQAAQKATPDDPLTIAAIRSRQYAASEITTLQTLGSRENYSDAIVSYRSDGLAIRAL
                     VSTPNAPKPAAGWPVVILDHGYINPTEYQTNSSDYREFIAAFTRAGYMVVKPDYRGHG
                     LSEGSPEGGHYSPAYAYDNLNLVASLQQFNQVDKSRIGLFGHSLGGHIALRTIVVSPD
                     VKATVIMSGVVGSIDDLFYNWTRSPNARPPTGLIRSARAALVEKYGEPRANPDFWNNV
                     SAINYVDAVTGPVQIQHSEGDSVVPKLFADHLN"
     gene            complement(934..1749)
                     /locus_tag="VF272_00790"
     CDS             complement(934..1749)
                     /locus_tag="VF272_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7259450.1"
                     /translation="MVPDEPPSAVDGWFCYATNMRIILVFIGLFIVVLLLVLLVEVWL
                     AMRNRPPAYRNVSPNAEFGQSGPVMHYVVMGDSTAAGEGAPYEAGIAVGSARYLAAQY
                     QVKLTNVGVSGAKTADVEGDQLAQAAALKPDLVLISVGANDVVGRRSPQAVEAGLRRI
                     TDRLVAANCKVKIVLTGSPNMGTITRFAQPLRTVAGLQTHHLNSRLYPWITASGYTLA
                     PIARDTTAAFRDDPSLFGSDRFHPNARGYAVWLKTINPALNQALADQLSHCKE"
     gene            1756..2685
                     /locus_tag="VF272_00795"
     CDS             1756..2685
                     /locus_tag="VF272_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX7259451.1"
                     /translation="MITTYYRNIKDAKLVQLDKFRTGSWIHMQAPTEAELAQVIEKFN
                     LEAGHVRDALDPDEMPRLETEDDTTYAFMRYSYNHDGRLLTAPVALIVGPKVLITIAH
                     QPFPRLELFTGGKLEVFTTQRAKLFLQLMRHITNTYNTGLNTINRQIRNARAGLNVTS
                     ISNRNFVQFVVIEDVLNDYLSELLPTNTMLTNLLSGKHSLSFFEEDRDLIEDLVLGTR
                     QSVEASKSNLRTIVNIREAYSNIMTNNLNRQIKLLTSLTIILMVPNVIAGFFGMNVPI
                     PLQDVPWAFPLILLSTLGLCGYLVWLFNRRGWL"
     gene            complement(2698..>3123)
                     /locus_tag="VF272_00800"
     CDS             complement(2698..>3123)
                     /locus_tag="VF272_00800"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7259452.1"
                     /translation="ELVQDLETILIESTHSHIAFPETLNALKSLSRDYRLAMITNTFQ
                     QGFEKLTNTFSIHDYFEIIITSYEEHFVKPDQHLFRTLFQRAGVKAEEILYVGDSVRS
                     DMNPARRLGMQTVLIDRRKRYPQYPRRITSLTQLAELAL"
BASE COUNT          784 a          819 c          753 g          767 t
ORIGIN      
        1 gtttaaatgg tcagcgaaca atttaggcac cacgctatcc ccttcactat gctgaatttg
       61 aaccgggccg gtaaccgcat caacataatt gatagccgat acattgttcc aaaaatcggg
      121 attagctcgc ggttctccat acttttcaac cagcgctgct cgggcactcc gtatgagccc
      181 ggttgggggg cgagcgttcg gcgagcgcgt ccagttataa aataggtcgt cgatggaacc
      241 gactacacca gacataatta cggtagcttt cacatccggt gaaaccacaa tcgtacgcag
      301 tgcaatatgg cctcccaggc tgtggccgaa caagccgatg cggcttttat caacttggtt
      361 aaactgttgt aagctggcta ccaagttgag attgtcatag gcataagccg gagagtaatg
      421 gccgccttcc gggctgccct cgctcaaacc gtggccgcga tagtccggct taactaccat
      481 atagcccgcc cgggtgaagg cagcgatgaa ctctcggtaa tcactactgt tggtttgata
      541 ctccgtgggg ttaatataac catggtctaa aataacgacc ggccatcctg cggccggctt
      601 gggggcgttt ggcgtactca ctaaagcccg gatggctaaa ccgtccgacc gatacgaaac
      661 gattgcatcg ctataattct cacggctgcc caatgtttgc aaggtagtta tttcacttgc
      721 cgcatactgc ctggaccgga tcgctgcaat ggtaagcgga tcgtcagggg tggctttctg
      781 agccgcttgg ggtgaagcag ctggcttctt ggcagagtta gatggtggct tgaatatata
      841 gaggataaga ataaatgcca acccgcttat aaccaagccg ctaataacaa atgatagctt
      901 ttgtaacttc gtaagccctt ttatcatctt aaattattcc ttacagtggg atagttgatc
      961 tgccaatgcc tgattcagcg ctgggttaat ggttttcagc cagaccgcat agcctctggc
     1021 attgggatga aagcggtcgc tgccaaacag gctcgggtca tcccgaaagg ccgccgtggt
     1081 gtcgcgagct attggtgcaa gcgtatagcc gctggccgta atccagggat aaagccggct
     1141 gttgagatga tgggtttgca aaccggctac cgttcgcagc ggctgagcaa accgggtaat
     1201 cgtgcccata ttaggcgagc cggtaagtac tattttaacc ttacaattag ctgccaccaa
     1261 acgatcggtt atccgccgta aaccggcttc caccgcctgt ggcgagcgtc ggcccacgac
     1321 atcattcgcc cctaccgaaa ttaataccaa gtccggcttc aacgctgctg cctgcgccag
     1381 ctgatcgcct tcgacatcgg cggttttggc tcccgagaca cccacattgg ttaatttcac
     1441 ttggtattga gccgccaagt agcgggccga tcccacggca ataccagctt catatggagc
     1501 gccttcgcca gcggccgtac tgtcgcccat tactacataa tgcatcacag ggccggactg
     1561 gccgaattct gcgttcggcg aaacattgcg ataagcaggc ggacgattac gcatagccag
     1621 ccaaacttct acgagcagaa ccagcagcaa aaccacaata aacaacccga tgaaaaccaa
     1681 gataatgcgc atatttgtag catagcagaa ccacccgtct accgcgctgg gaggctcatc
     1741 tggtaccata ggggaatgat taccacctat tatcgcaata ttaaagacgc caaactggtt
     1801 cagcttgata agttccgaac cggttcgtgg attcatatgc aggcacctac tgaagccgaa
     1861 ctggctcaag taattgagaa gtttaacctc gaagccggcc atgttcggga tgccctcgac
     1921 cccgatgaaa tgccgcgctt ggaaaccgaa gacgacacca cctatgcctt tatgcgctat
     1981 tcctacaacc atgatggacg tctgttaacc gcgccggtag ccttaattgt cggtcccaag
     2041 gtgcttatta ccatcgccca tcaaccgttc ccccgcctcg agctctttac cggtggaaag
     2101 ctggaggtat tcaccaccca acgagctaag ctatttttac agctgatgcg gcatattacc
     2161 aatacctaca ataccggact caataccatt aaccgccaaa tccgcaatgc ccgagccggc
     2221 ctcaatgtta cctctattag caaccgcaac ttcgtgcagt ttgtggtaat tgaggatgtg
     2281 ttgaatgatt acctttcgga actgctgccc accaacacca tgctcacaaa cttattatca
     2341 ggcaaacata gcctgtcttt ctttgaggag gaccgtgatt taattgaaga tttggtgctg
     2401 ggaacccgcc agtcggtaga agccagtaag agtaatttgc gcaccattgt aaacatccgc
     2461 gaagcctatt ccaacattat gaccaacaac ctgaaccgtc agattaagct gttaacctcg
     2521 ctgacgatta tcttaatggt gccgaatgtc attgccggtt tcttcggtat gaatgtaccg
     2581 attccgctgc aggacgttcc gtgggcattt ccgctgattc ttcttagtac cttgggtttg
     2641 tgcggctatt tggtctggct cttcaatcgc cgcggctggc tataaaagtt aaggcgatta
     2701 taaagccagt tccgctagtt gcgttaagct ggtaatccgg cggggatact gcggatagcg
     2761 tttgcgccgg tcaataagca ccgtttgcat tcccagacga cgcgccggat tcatgtcgct
     2821 gcggacactg tctccaacat agaggatctc ttcggccttg acccctgccc gctgaaataa
     2881 cgttcgaaac aaatgctggt ccggctttac aaaatgctct tcatacgacg tgataataat
     2941 ttcaaaataa tcatgaatgc taaatgtatt cgtgagtttt tcaaaccctt gctgaaaagt
     3001 gttggtaatc atcgccaacc gataatcccg agaaagtgac ttcaacgcat ttaacgtttc
     3061 tggaaaggca atatgagagt gagtggattc aatgaggata gtttccaaat cctgaaccag
     3121 ttc
//
LOCUS       DASVGB010000030         3546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_819530, whole genome shotgun sequence.
ACCESSION   DASVGB010000030 DASVGB010000000
VERSION     DASVGB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819530"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            706..1317
                     /locus_tag="VF272_00805"
     CDS             706..1317
                     /locus_tag="VF272_00805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7259453.1"
                     /translation="MIITSSKSEITMNNFGIYRQVQGNPANSYADWLILDYNTFMTST
                     AAQLRRAQLRDIAPLRTLFVHALETDFDYFPHRYLRQVKRQHSVPRLVVSLLKKQRII
                     LIAALDKRIVGYMVGSGHQDGVGELYWLYVHPSQRGQRVGTKLLQQAIGELRQQGMHT
                     VRLMTHKFERYYKRYGFEVCGTYAIESLEVKIMQLRFEGSTHG"
     gene            1310..2620
                     /locus_tag="VF272_00810"
     CDS             1310..2620
                     /locus_tag="VF272_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX7259454.1"
                     /translation="MDRHRRPKPRVRPRYIAAFWALLIGLFVLQAFQFLMGATELPLM
                     RSPLPQAPGAGTIIEVVETRHFAAAEVTQAARQNYGTAFRASNHGVTRVLVRYRSTGQ
                     NNALVPIYGRAYIPDKATNAPVLAMAPGTTGIGDGCAASLEQPKVRNWANYESHMMAY
                     ASRGYGGIITDYEGMRDPARIHHYMVGVLEGRAVLDGVRALKNLPQAHQLNTKQVFAG
                     GYSQGGHSVYWADSVAVDYAPDVELAGIIGWSPVMDVAETWRGVTAGSTLSWYGPYVL
                     TSYSDFYGRNYNLPNILLPQWLPNLPTDVNNHCIDTNLGFWGIDPVKVFTPQFLADLR
                     TGNLPVERYGMLQTDLEANQVKGDRSTTPKLINQGMRDNVVLPSQQPPALARLCAESR
                     APASLKQYANSNHYTVMRDSFNDTLAWMEQIRNRQRTASTCPAN"
     gene            complement(2617..>3546)
                     /locus_tag="VF272_00815"
     CDS             complement(2617..>3546)
                     /locus_tag="VF272_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7259455.1"
                     /translation="NMRLETIPTFHGEEIVVRIFNTTTVNLTLATMGFTEAQTKRLDV
                     ITSHQNGMLLTVGPTGSGKTSTLYALINRLNNAEIKIATLEDPVEYDLPGISQIPVRT
                     EDQQLFAEKLRAVMREDPNIVMIGEIRDIDTAKTSLQAALTGHLVLSTFHASNAAAAI
                     SRLLDMIGQNPLFASAIRLIIAQRLVRRICTACIIEADATEEELVLLKQAISELAADR
                     RPTLEATPKLKHGKGCAVCHGLGYKGRIAVIEMMPIDPDIEQLMTSTAKTTAQDLEAK
                     AIANGMVTLLEDGVHKVLEGVTTIQEIQSLTAT"
BASE COUNT          853 a          858 c          927 g          908 t
ORIGIN      
        1 gtagtggtag gggcgggcaa taacagctgg cctcccatca tggcaattag cactaggcta
       61 aagcaagatg ctatagctgc aaggcgataa atctgggatg tagggctctt cattggtatt
      121 tttgtttttg ctgagaataa gctcagaacg cttatgctta aaatataatc cctgatcgct
      181 ggttatgcaa gtgctttagt gcttatgagc gcgaagagat ttcgaagacg cgcaatctca
      241 tggttcatcg ctcgccttcc aaatggagtt agaccatacg attgacgacg caggctacga
      301 gggaacggca atggagcatg ttcgctaacc cagccgcgct tgaccaggcg ccttaaaagc
      361 ctgcttaccg ttgagcggtt cggtgccacg attccttcgg agtatttcgc aatctgttcg
      421 ccaaccatgt aggcgtgtag ttcgccgccg gctaatgagc acagtgctaa taaggttgtg
      481 ggcgacatgg gggtccacat ggaccgtttc atatgcatta cagtgagttc ctttattgaa
      541 attcttattt tagtgtccca aggatactaa aattggatta aaattatctg aaatgggctc
      601 aaaaatagta tccttgggac actatatata gaagaatatg ttgaggtgct ctagccaagt
      661 acgccggtga tcaccgatca cttgattaaa cctaggctgg atcatatgat aatcacaagc
      721 agtaaatctg aaataaccat gaataatttc ggaatctaca gacaggtgca ggggaatcca
      781 gcaaacagct atgcagactg gttgatactg gactacaata cctttatgac ttctaccgcg
      841 gctcagcttc gtcgggccca attacgtgat attgcaccgc tgcggacatt atttgttcac
      901 gccctggaaa ccgattttga ctattttccg catcgttatc tcagacaggt aaagcgccag
      961 catagcgttc cgcgtctcgt tgtatcattg ttgaaaaaac aacgaattat tttgattgcc
     1021 gcgctcgata aacgtattgt cggatacatg gtgggcagcg gtcaccagga cggtgtcggt
     1081 gaattatact ggctttacgt gcatccttca caacgtggcc agcgagttgg aacaaagctg
     1141 ctgcagcaag ctattggcga actcaggcag cagggaatgc ataccgtaag gctgatgacg
     1201 cacaaatttg aacggtatta caaacgctac ggttttgaag tatgcggcac ctatgccatt
     1261 gaaagccttg aggtaaaaat aatgcagctg cgttttgaag ggagcacgca tggatagaca
     1321 tcgccggcct aagcctcggg ttcgtcctcg ttatattgcg gctttttggg cgcttttaat
     1381 tggtctgttt gtattgcagg cgtttcagtt cttgatgggc gcaaccgaac tgccgcttat
     1441 gcgttcgcca cttcctcagg cccctggtgc cggaaccatt attgaggtag tcgaaacccg
     1501 tcactttgca gccgccgagg ttacccaagc cgctcgccag aactatggaa ccgcttttcg
     1561 ggccagtaac catggggtaa cccgggtgct ggttcggtat cggagtaccg gtcaaaacaa
     1621 cgcattagtg ccgatttatg gacgggccta tatacctgat aaagctacaa atgcaccagt
     1681 tttggctatg gcgcctggta caaccggtat tggtgatggc tgtgccgcca gtttggagca
     1741 gccaaaggtg cgcaactggg ccaactacga atcacatatg atggcctacg ctagccgcgg
     1801 atacggcggg atcattaccg attatgaagg aatgcgcgac ccggcccgca tacaccacta
     1861 tatggtgggc gtgcttgagg gtcgggcggt tcttgatggc gtgagggcgc tgaaaaacct
     1921 ccctcaagct catcagctta atactaaaca ggtttttgca ggtggatact cgcagggcgg
     1981 acatagcgta tattgggctg atagtgtggc cgtcgattat gctccggacg ttgaactggc
     2041 cggtattatc ggttggtctc cggtcatgga tgtggcggag acatggcgcg gggttactgc
     2101 cggctcaaca ctcagctggt acggtccgta cgtgcttacg agctacagcg acttttatgg
     2161 ccgtaattat aatctgccaa atattttgct tccccagtgg cttcctaatt tgccgaccga
     2221 cgtaaacaat cattgcattg acaccaacct cgggttttgg ggtattgatc cggtaaaagt
     2281 ttttacaccg cagtttttag ctgatctgcg caccggtaat ctgccggttg agcgctatgg
     2341 aatgctgcag accgatcttg aagccaatca ggttaagggt gatcgttcga ctacgcctaa
     2401 gctcataaac caaggaatgc gtgataatgt cgtcttgccg tcgcagcagc caccggcact
     2461 tgcccggctc tgcgctgaaa gccgtgcgcc agcatcacta aagcaatacg ccaactctaa
     2521 ccactacacc gtgatgcgtg acagttttaa tgataccctt gcatggatgg aacagatcag
     2581 gaatcgccag cgcacagcca gcacctgccc ggctaattaa gtggcggtta gactttgaat
     2641 ttcttgaatg gtagtaacac cttcaagcac tttatgcact ccgtcttcaa gcagcgtaac
     2701 catgccattg gcaatagcct tggcttccaa atcttgggcg gtggttttgg ccgtggaggt
     2761 cattaactgc tcaatatccg gatcgatcgg catcatttcg atgaccgcaa tccggccctt
     2821 atatcccagg ccatgacaaa ccgcacaacc tttaccatgc tttagcttgg gtgtagcttc
     2881 cagtgttggg cgccggtcgg cagccagctc actgattgcc tgtttcaata acaccaactc
     2941 ttcttcggtt gcatcggctt caatgataca ggcggtgcag attcgccgga ccaagcgctg
     3001 ggcgataatt aacctgattg ccgaagcaaa aagcggattc tggccgatca tatccagcag
     3061 tcggctaatg gcggcagcgg cattagaggc gtggaaggtc gatagcacta agtgtccggt
     3121 gagtgccgct tgaagcgagg ttttagcggt atcaatgtcg cggatctcgc cgatcatcac
     3181 aatattggga tcttcgcgca tgaccgctcg cagcttctcg gcgaacaact gttgatcttc
     3241 ggttcgaacg gggatctggc taatacccgg cagatcatat tccaccggat cctccagggt
     3301 ggcaatcttt atctcggcat tattaagccg gttgatgagg gcgtataagg ttgaagtttt
     3361 acctgacccg gttggcccga cggtcaacaa catgccgttt tgatgcgagg taatcacatc
     3421 caagcgcttg gtttgagcct cggtaaagcc catcgttgcc agggtcaagt taaccgtggt
     3481 ggtgttgaat attcgcacta caatctcttc accatgaaag gtcgggatag tctccagacg
     3541 catatt
//
LOCUS       DASVGB010000031         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_392098, whole genome shotgun sequence.
ACCESSION   DASVGB010000031 DASVGB010000000
VERSION     DASVGB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392098"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..709)
                     /locus_tag="VF272_00820"
     CDS             complement(50..709)
                     /locus_tag="VF272_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259456.1"
                     /translation="MTTIYSPSFSAQNYVAPPVPGKPKTPKSYIKRIIFGIAVFVVVV
                     GLLVMAYFIFLGNSSVKGEVKTVPIRENTDLSSRSKQLSLKGQYFSMNYAPVYATQDT
                     QVRTNNVFESFSYVASTVYDKRMAVAIQKQPTGGIMEESSYKMRTIYTDKYTERKTKT
                     GDGLSAIVMTKKTDGTNKESVLFISNNSLLATIALVVTGGDDDLEKEIDKIAASFKWN
                     K"
     gene            complement(706..1692)
                     /locus_tag="VF272_00825"
     CDS             complement(706..1692)
                     /locus_tag="VF272_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259457.1"
                     /translation="MREKLLQHFAPHHHSGRLVHHSHTSYAGLMFVIMLACIILMAFT
                     MNVQAFDPSELPPPVTASTDVHGTVPGPAPKTAPTIQTPSNGQVVSSVPVSVLGKCQK
                     DTLVKIFKNDIFAGGAFCDGTGSYKTSIDLLVGPNTLTAQSFNALDKGGPVSGAVNIT
                     YNLPAQTAAQVPGAFEPAKTPANQLLINTVNYHRGLRVGDELSWPLELVGGKPPYAIS
                     VGWGDGKTDLLSRGAQGRFDITHVYEKPGSGHNGSQTIVVKATDADGTTAYIQLVTIV
                     SNGSKQLAAANSLLSGNLGIAWPLVLLAFIVVVSFFLGERREKHILHQKGLI"
     gene            1771..2904
                     /locus_tag="VF272_00830"
     CDS             1771..2904
                     /locus_tag="VF272_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259458.1"
                     /translation="MDTSAPDNKPDSKNPAAKAPEAAAAPKQADTADASKPTENQAKG
                     TEGEVLRDDKSGYLYQPHTVETPIEKHTNAKKFIKPKFLIPGSIILILVIAGVAYLIL
                     NDKKSDTQKSLEKAGISVQNVSSDTITNLAAAIGIGQPKQTLTISADTIFKNQVTLEK
                     GLTSGGLINANSGIRISGAASAETLNLTSNLSVNGTTSLQGIVDVRNQLNVGGPLNVT
                     GSGSFAGNINARGSATVGGNLSVSGTFAAGTISAQTLTISQTLTVNGHIVSGGSAPSI
                     SATTRIGGGGVASISGNDTAGTISITTGTTPNCGPSGNGQWATISFSRPFSSVPKVVL
                     SPVGFNSAKIEISAIRTPQNFTISCNVPPIANTAYAYDYIVVE"
     gene            complement(2911..>3753)
                     /locus_tag="VF272_00835"
     CDS             complement(2911..>3753)
                     /locus_tag="VF272_00835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7259459.1"
                     /translation="PVLHTIHNSFFNDNRSIKVPDNHILYEQLSQSRRIWFNGISNAQ
                     VSNAPPLLRQKGIPVMYNALDPDLYPFQGNKSDFFLTMGRFTQDKGQAVAARLSAELG
                     YTLKMAGSINGLSSPRELEAVRQDPSHPTNLHPDYMYYQREVAPHVMAGAIEYVGNVS
                     GNYKLKLMAEAKALLFPIDWEEPFGMAVIEAMACGTPVVAMRRGAMPELIEHGVNGYL
                     ADTESEFKHYMQLVDEIDPEACRATVEERFSADHMAEEYLNLYTKVMVADTRLPDSLT
                     KILQ"
BASE COUNT          980 a          983 c          862 g          928 t
ORIGIN      
        1 caaacagcat ccaagaacct aatacaatat gctgcagtac atccaccggt tatttattcc
       61 acttaaagct cgcggcaatt ttgtcgattt ctttctccaa atcatcatca cctcccgtaa
      121 caaccaaagc aatcgttgct agcaagctat tattgcttat aaacagtact gactccttat
      181 tcgtaccatc ggtttttttg gtcataacaa ttgccgataa gccgtcaccg gtcttggtct
      241 tccgctcggt gtatttgtcg gtgtatatcg tacgcatttt ataagaagat tcctccataa
      301 tgccccctgt tggctgtttc tgtatagcaa cagccatccg cttgtcgtac acggttgaag
      361 ctacatagct gaacgactca aaaacattgt tcgtgcgtac ttgagtatct tgggtagcat
      421 acaccggcgc atagttcatg ctaaagtact ggcccttcaa agaaagctgt ttcgaccgcg
      481 aagaaagatc ggtattctcc cggattggga ccgttttaac ctcacctttt acgctcgagt
      541 tgcctaaaaa tataaaataa gccattacca atagtcctac cacaactaca aagacggcta
      601 tgccgaagat gattcgctta atatagcttt taggcgtttt tggcttcccc ggaacgggag
      661 gtgcaacata attctgtgcg gaaaatgaag ggctgtagat cgtagtcata tgagaccctt
      721 ctgatgaaga atgtgttttt cacggcgttc cccaagaaag aaacttacca caactatgaa
      781 agctaggagt accagtggcc aagcgattcc cagatttccg gacagaaggc tgttggccgc
      841 agcaagctgt ttactgccat tactaacaat cgtaactaat tgaatgtaag ccgtcgtgcc
      901 gtcggcatcg gtagctttca ctacaatggt ttggcttccg ttatggcctg atcccggctt
      961 ttcataaaca tgggtgatat caaatcgacc ctgtgcgccg cgactcaaaa ggtcggtctt
     1021 gccatccccc caacccacgc ttatcgcgta cggaggtttt ccgccgacca gctcaagcgg
     1081 ccaactaagc tcgtccccta ctctcaatcc ccggtggtag ttgacggtat taataagcaa
     1141 ttgatttgcg ggagttttag ccggctcaaa agcaccgggc acttgagcag ccgtttgggc
     1201 gggcagatta taggtaatat tgaccgcacc ggaaaccggt ccgcctttat caagtgcatt
     1261 aaacgactga gcggttaatg tgttgggtcc gaccaacaag tcaatggatg ttttatatga
     1321 tcccgtgcca tcacagaacg caccgccggc gaaaatatca tttttaaaaa tttttactag
     1381 ggtatctttt tggcatttgc caagcaccga caccggaacg ctagacacta cttggccgtt
     1441 gcttggcgtt tgaatggtcg gagcggtttt cggagccgga cccggcaccg ttccatgaac
     1501 atcggtgctg gcggtaaccg gaggcggcag ttctgaagga tcaaacgctt gcacgttcat
     1561 cgtaaatgcc atcaaaatga tgcaggccaa cataataacg aacattaacc cggcgtatga
     1621 cgtgtggcta tgatgtacca accgcccgct atggtggtgg ggggcaaaat gttgcaacag
     1681 cttttctctc acactcctat tatacctctt gtgcttttac cctgccaacc ttgtgactct
     1741 taagcgtaag cggtatgatt aaggtagatt atggatactt ctgctccaga caacaaaccc
     1801 gactcaaaga acccggctgc caaagcaccg gaggctgcag ctgcccccaa gcaagccgac
     1861 actgctgatg cttctaagcc gacggaaaat caggccaaag gcaccgaggg cgaagtatta
     1921 cgtgatgaca aatcgggcta tctttaccag ccgcataccg tcgaaacccc gatagaaaaa
     1981 catacaaatg caaaaaaatt tatcaagccg aaatttctta ttcccggcag tattattctg
     2041 attttagtca tcgccggggt ggcgtatcta atattaaatg acaaaaaaag cgatacccag
     2101 aagtcgcttg aaaaagccgg tattagcgtg cagaacgtaa gcagcgacac tattactaat
     2161 ctagccgcag ccatcggaat cggccagccc aaacagacgt taacaatttc agccgatacg
     2221 atctttaaaa accaggtaac actagaaaaa ggcctcacca gcgggggact catcaatgcc
     2281 aattccggca ttcgtatttc cggcgcggca tccgctgaaa cgcttaatct tacctcaaat
     2341 cttagcgtga atggcaccac cagcctgcag ggaatcgttg acgtacgaaa tcagctgaac
     2401 gtcggaggcc cgcttaatgt tactggcagc ggaagttttg ccggcaatat caatgcaaga
     2461 ggcagcgcta ccgtcggcgg caacctctcg gtgagcggca cgtttgctgc cgggacgatt
     2521 tcagctcaaa ccttaaccat tagccagacc ctcactgtta acggccatat tgtgagcggc
     2581 ggttcagcgc cgtcaatatc ggcaactacc cgtattggcg ggggcggagt ggcgtcgatt
     2641 agcggcaacg acaccgccgg aaccatttcg attactaccg gcaccacccc gaactgcggt
     2701 ccgtccggga acggccaatg ggcaaccatc agttttagcc gcccattcag cagcgtacct
     2761 aaagttgtac tctcaccggt tggttttaac agcgccaaaa ttgaaatctc ggctatccgg
     2821 acacctcaaa actttacgat ttcctgcaac gtgccgccca tcgccaatac cgcctacgcc
     2881 tacgactaca ttgtggttga gtagcacacc ttactggagt atcttggtta atgaatctgg
     2941 cagccgcgtg tcagcaacca tcaccttggt ataaagattg agatactctt ctgccatatg
     3001 atcggccgag aacctctcct ccacggtcgc gcggcaagct tcagggtcaa tttcatcaac
     3061 cagctgcata tagtgcttaa actcggattc ggtatcggct aagtagccat taacgccatg
     3121 ttcaatcagc tccggcatag ctccgcgccg catcgcaacc actggagtcc cgcaggccat
     3181 cgcttcaatc accgccatcc caaacggctc ttcccagtca atcggaaaca ataaggcttt
     3241 agcttcggcc attaacttca atttgtaatt tcccgaaaca ttaccaacgt actcaatggc
     3301 tccggccatt acatgaggcg caacttctcg ctgatagtac atataatcag gatggagatt
     3361 ggtggggtgg ctgggatcct ggcgtaccgc ttcgagttcc cgcggactgc taagtccgtt
     3421 aatactgccg gccattttaa gcgtatatcc cagctcggcg cttaatctgg ctgcaaccgc
     3481 ctgccccttg tcctgtgtaa aacggcccat ggtcaagaaa aagtcactct tattaccctg
     3541 gaagggatag agatcaggat caagggcgtt atacatcacc ggaatacctt tttgccggag
     3601 gagcggtggc gcattgctaa cttgggcatt cgatatgccg ttaaaccaaa tgcgccttga
     3661 ttggcttaac tgttcatata agatgtggtt gtccggcact ttgattgagc ggttatcatt
     3721 aaaaaatgaa ttatgaatgg tatgaaggac cgg
//
LOCUS       DASVGB010000032        23666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_960024, whole genome shotgun sequence.
ACCESSION   DASVGB010000032 DASVGB010000000
VERSION     DASVGB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 23666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23666
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960024"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..626
                     /locus_tag="VF272_00840"
     CDS             108..626
                     /locus_tag="VF272_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259460.1"
                     /translation="MNKRNGLIAGAVIAVVAIFIGGYELGARTVESAEPPAAATTPQP
                     VKADKPVRAHAQLYIDNHLGFQMEVPFEWVNRYAPIQSGSSVGFAFEKATSPPGTTKM
                     DLVQIFTITRYPAVNFNEAACGCKEIGRNKNAVFGYKFDGQNVALAQPLTVANIQAAM
                     QNTIPEFFKTLD"
     gene            707..1396
                     /locus_tag="VF272_00845"
     CDS             707..1396
                     /locus_tag="VF272_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX7259461.1"
                     /translation="MTPRQQYILSEIVETYARTAEPVSSNLLTDKIEVSSATIRADMA
                     ELERLGYILQPHTSAGRVPTDSGYRMYVNNVKELHSDGRINQVLARRVSSAGEADRAI
                     KQAAESLADITQNLGLATLSEGLVFTGLRNLFGQPEFSGTKAFEVARLLDSLDEWLAE
                     AAPSGRVSVYIGTENPIGRSSGATLVIARFASPHSDSSYIGVLGSTRQNYTRVMGLVD
                     YAGRYLEETLV"
     gene            1393..1902
                     /locus_tag="VF272_00850"
     CDS             1393..1902
                     /locus_tag="VF272_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7259462.1"
                     /translation="MTKKPISKKPSVHELQERVDELTNDLQRLQAEFINYKRREEDAK
                     SDIASFATVRVVKELLPLLDNIERALAHRPEELNDNDWAQGVEHVGKQTLEVLKVLGV
                     EKIQSVGEPFDHNRHEAISMEDGEGDTEIVIDEVQAGYTMGDQVIRHAMVKVGKGNLR
                     NNEEGETNG"
     gene            1895..3850
                     /gene="dnaK"
                     /locus_tag="VF272_00855"
     CDS             1895..3850
                     /gene="dnaK"
                     /locus_tag="VF272_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7259463.1"
                     /translation="MGKIIGIDLGTTNSAMAVMEGGQPTIIANAEGGRTTPSIVAINK
                     NGERLVGQAAKRQAVINGENTIFEVKRLIGRKFADDEVQKDVGLMPYKIVDANGSVKV
                     TMGDKDYSPEEVSAMILSKLKADAEAFTGGPVTEAVITVPAYFSDAQRQATKDAGKIA
                     GLEVKRIINEPTAAALAYGLDKKAEEKIVVYDLGGGTFDVSILELGEGVFEVKSTNGD
                     THLGGADFDNTVMTFLIDEFKKDQGIDLKDDKAAVQRLKEAAEKAKIELSSAQDTEIN
                     LPFITADATGPKHFTYHLTRSKLQQLVQGLIDKTAGPCEAALKDAGLTAADINEVILV
                     GGMTRMPAVAEKVQAIFGKEPLKGVNPDEVVAVGAAVQGGVLAGDVKDVLLLDVTPLS
                     LGLETMGSVMTKLIERNTTVPTSKSQVFSTAADSQNQVEINVLQGEREFAADNKSLGR
                     FILDGIPPAPRGVPQIEVTFNIDANGIVNVKAVDKGTNKEQHITITGSGNLSDEEIKK
                     AQAEAEVHAEEDKKRKAVVEARNNLDNTIYTAEKTIKDAGDKAKEEDKTALQTAIDEA
                     KKHNETDDAAVLEKAAKDLSDKMQVVGAAMYAADDATGSGATASDTAASGGASASASA
                     SAETTTSEPVEGEVVDEKKDGTPEAGK"
     gene            3873..4979
                     /gene="dnaJ"
                     /locus_tag="VF272_00860"
     CDS             3873..4979
                     /gene="dnaJ"
                     /locus_tag="VF272_00860"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7259464.1"
                     /translation="MAKRDYYEVLGISKSASADEIKRAYRKLAMQHHPDKHGGDDGPF
                     KELGEAYEVLKDEQKRAAYDQFGHAAGAQNAGGPNPFGGGFGAQGFDFSQFQGQGGGF
                     GDIFDMFFQGQGGGSRRQSARGTDMETVITLDFKEAIFGATRKLSLTTDERCDHCDGK
                     GGEPGTKIKTCDTCKGQGQVTRVQQTILGAIQQTTVCPTCRGEGEIPEEKCSKCRGAG
                     VIKKTKNMEVKIPAGIDNGQTIRLDGKGAAHKKAPNGDLYIHVQIRPDSKLVRHGQDI
                     SSTIKVDMVGAALGSEVPVHTVDGDVKLKIPAGTQSGKIFRLSERGVPGIGGRKRGDH
                     LVTVTVETPSKLSAKQKELLEQFASEGPKKRKFW"
     gene            complement(4980..5594)
                     /locus_tag="VF272_00865"
     CDS             complement(4980..5594)
                     /locus_tag="VF272_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259465.1"
                     /translation="MARLQALDQDPERIQWFESRPENYVHLTSEELIEFSQHGDDHLL
                     FAVAGSEQHPTLDKSEVGEVQGWVKLNPDAEMRVSQLREQHIIPSDQPAENIWEVSYT
                     KLEGAQEKQMASGLRQSLIAVSQLDAARTNNQVLEPQITVAAYVIDGNTRSEQVLLAS
                     GFEEKGRIQYDDEASQPDRLFILNWDKLTEAMQKAAYDELKEPA"
     gene            5796..6329
                     /locus_tag="VF272_00870"
     CDS             5796..6329
                     /locus_tag="VF272_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259466.1"
                     /translation="MVTLLSTIPAAVPDFVSALSLDAAIVVTTLLLVVYSVVRGHMAL
                     IREAISIYVGLVLSASLGEPVYDYLHNAAASLPLNETAVKLFLLLAPVVVLQIGRHHP
                     RGGHKHHLIITFVLAVMTGFLLISSVLNQLDQVTLSHALENSNLAAWIYEFKLVWLGA
                     VPLAIAASAFLRPKGHH"
     gene            6392..6838
                     /locus_tag="VF272_00875"
     CDS             6392..6838
                     /locus_tag="VF272_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259467.1"
                     /translation="MPRKTKHLSLSSQVRFKFSHVLMFAAAFAALGIYAVLHTFAAPV
                     STLSECTVVPSSAKAGDSHLTVTAPVGELPENALGENYRVKLETPYINEQLVDAVYDA
                     ANGIAILNWNGLLVKGKHSFYFVYVTGHTGNSQVVRASRTCSINVN"
     gene            6895..6971
                     /locus_tag="VF272_00880"
     tRNA            6895..6971
                     /locus_tag="VF272_00880"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6929..6931,aa:Lys,seq:ttt)
     gene            6987..8405
                     /locus_tag="VF272_00885"
     CDS             6987..8405
                     /locus_tag="VF272_00885"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7259468.1"
                     /translation="MPKHLYLIINPDSGTGDPKAVLSEVSAELDQRTIEYVLKKVETP
                     HEAFTAAQAIDLKKFDAVGVYGGDGTIIAVFKALYKQKTPVLIMPGGSANVLAKALNL
                     PASAGDCLDLFLGNLYVLRHFGVAMANDAPLILDLHFGIWADSIEATNQRTKQRFGQL
                     AYAFSAIKHLGSASRFNFQMTLDGKQVDVSAYACMVANEGYHTIIGVPFFPNSHTPGF
                     LQVAAVKRVNQWAFLRWLTLRRLSIPTSSVVATWRVKEVVIQRAPKQMMFDDDIVTAT
                     LPLTIKSSPYMVAVIASTPDKSTFWRRLQMYAKVEPFRRYDHLRRFFRGGPSLKSSQI
                     APNLYVGGQYRPWAIARLRRWGVTGVVNMRNVRRPSNSRGLEVLNLPTPDHQPPSLAS
                     LYKGVEFIQHHLDAGGAVYLHCRLGEGRGPSMAAAYLISQGMRANDAVAHIERFRRLI
                     RPNAKQLRQLARFEAELNQKKV"
     gene            8500..9747
                     /locus_tag="VF272_00890"
     CDS             8500..9747
                     /locus_tag="VF272_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259469.1"
                     /translation="MDIAPQTNSTPSVSPKWGGLLSYGLISACIASFISIFLFLVAQA
                     SFNQVSELNKTLAHDLSSPIIMLTYTLWWSAGAFILFGTICSASWFLLLRVDPTTIPW
                     KRSIIINSIQWFALAFLLLKAASTIPDEWKQLMASSWAAFFFVFAFQIAPAIAGAAIV
                     IVMFWRNRLRATRSPAPPPTEIEQATQIPLPQKNFLSRTTALLGFILFAPIFVIASVI
                     ALSGSILFVYGLITGKFDEGISHLIIFGWTYFAYGVASATGVPAIGLAMYLFRSKGPL
                     VSAGDRNKRALLIGSPIIVGLGLIVWFMQPPTNTQKEADAYQDKKVIACFKIPDGGTY
                     NAVSNTVKHIPNREVAPVVDLGYYSNYAERHDDVLGIKLSPSSRKDAEEDVKIIRQNY
                     LDKISIIGIYNKNAIPPHMCGIK"
     gene            9789..11003
                     /locus_tag="VF272_00895"
     CDS             9789..11003
                     /locus_tag="VF272_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7259470.1"
                     /translation="MLGHRNFSALRNYNYRIYFTGMSISLIGTWAQVIALSWLVLQMT
                     GSGTALGLIHAAHFLPLLILGPWGGLVADRRTKHKLLLATQASSAILSLLLGVLVVTG
                     TVQLWMVFVITLGLGLVRVVDHPTRQALIFEMAGKDHVQNAMVLQGTSVSVTRMLGPA
                     IAGVLIATLGIGMCFIINAFSYIPMLVAMALLKVDELEVAPLIEKASGQLREGFRYVM
                     KTPVIRNNLIMMAIIGTLAYEWAVVLPLLASFTFHGEAADYALLMTALGIGATIGGLY
                     TARYRSVTQPMISLAALMLGAMVVLVALAPSLLFAVVAMGLVGFFAVQFDSLNSSILQ
                     LTSSPEMRGRVMALWGVAFLGSSPIGGPLIGWICQLAGPRTGLLIGAVAAMAAATFGY
                     VTTRRSSVILKT"
     gene            11028..11993
                     /locus_tag="VF272_00900"
     CDS             11028..11993
                     /locus_tag="VF272_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7259471.1"
                     /translation="MSERKPHRPKRISARRVVITSFVVDLGDIILNVITAVLTGSVVV
                     LTEALQGAADLLTSALLWVGVQRSKRQATRVHRFGYGKELFFWILMAGVSMFIITAGF
                     SFYFGFQRILHPHPIQNIELAYVVLTIGFATNLYALSLSLRRLGVSLRHKLSHIRRHI
                     RNSALIETKATTILDLMGASAAMLGLISLVIYGITGESRFDGLGAMVVGLSCAVFALL
                     LIFEVKNFIVGRSASPEVESQIKAVATNVVGVHSVLDLRTMQMGSERVMVNMEVHLDH
                     RLRTQEIEQLMDDLKEAVKKDVPEVQHIQVEVETPRRKPVRPSND"
     gene            11986..12561
                     /gene="purN"
                     /locus_tag="VF272_00905"
     CDS             11986..12561
                     /gene="purN"
                     /locus_tag="VF272_00905"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7259472.1"
                     /translation="MIKLAVLVSGTGSILEAMLQQEVPVALVVADRPCRGLELAEQAG
                     IPTALIDRTAFGFSGPGSEWDRTGFTQAIIEKLKEQDIGLVAMAGFMTILDPVIFEAF
                     PTRILNTHPSLLPAFKGDAAVQDALDAGASVTGCTIHYASADLDGGPIIVQREVEILP
                     GDTRESLHERIKTMERTMYPEVVKKLLAEDD"
     gene            12602..13996
                     /gene="purB"
                     /locus_tag="VF272_00910"
     CDS             12602..13996
                     /gene="purB"
                     /locus_tag="VF272_00910"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7259473.1"
                     /translation="MQGDFSSYNSPLGYRYASPEMRELFSEATKYKLWRRVWVALARA
                     EHQAGLINDEELADLEANQDTIDIARILEIERDTKHDVVAAIREYAEKAKIGGGKIHL
                     GATSMDINDNADAMRVKQALELIEDRLKALLKVVAAQADRHAETPCLGYTHLQPAEPT
                     TIGYRLAFYGDDLLSDLQRIQGLHYRFVGKGMKGAIGTRASYTAILEGTDMTAAQLDE
                     KVMAELHLRPALIATQVLTRKQDYDILSALAGIGGSLAKMGADIRLLQSPGFGEWSES
                     FSDKQVGSSAMPFKKNPITAEKICSLARQLAQLPAVALGNASHSYLERTLDDSANRRM
                     TTPEAFLLADEILITATKLIEGLTINGDRVAENLAQFGPFAATEVLIIEAVKQGADRQ
                     AMHELLRTVALEAWQQPQPGKAMVELLKDNAELGKHVTSERLDELLDVRTHLGDAPDR
                     VKQLVALIHKEVEG"
     gene            13993..15120
                     /locus_tag="VF272_00915"
     CDS             13993..15120
                     /locus_tag="VF272_00915"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7259474.1"
                     /translation="MNHRLGIVGGGQLGRMVAFDAKRLGFAVTVLDPEPNSPAGQVAD
                     EHIVAAYDDDAAVRRLAKSVDFITYDIESAGKGVLAELAENGKQVNPSPWTLNMIRDK
                     LGQHRWLREVGIPVAEFMEVETDEDIKQAGETFGYPLVLKARFDAYDGRGNAIIKSSD
                     EIASVKEKLAGRELYVEQTVPFIKELAVIAVRGMDGAIAVYDAVETIHENNICHMVYA
                     PARVEKTVVHKARELAEQVVGQLKGAGVFAVEMFISADGKVIVNEIAPRVHNSGHLTI
                     EAAVTSQFEQHVRAVTGLPLGDTSLKVPAAVMINILGERSGPANPEGLAEALAIPGVS
                     VHIYGKAKTWPQRKMGHITAIADTLEAAEQKALEARKAVTI"
     gene            15117..15650
                     /gene="purE"
                     /locus_tag="VF272_00920"
     CDS             15117..15650
                     /gene="purE"
                     /locus_tag="VF272_00920"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7259475.1"
                     /translation="MSEREQPQVGIIMGSDSDLPVMKQAAEVLESFGISAEVTVVSAH
                     RTPERLWEYATQARSRDFKVIIAGAGGAAHLPGMVASMTELPVIGVPIQTKTMKGIDS
                     LLSIAQMPPGIPVATMAVNGAKNAGLLAVRMLGLGDSAILDSMHDYQKSLEAEIKIKA
                     EKLEAVGYEDYLKEKGE"
     gene            15652..16620
                     /locus_tag="VF272_00925"
     CDS             15652..16620
                     /locus_tag="VF272_00925"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7259476.1"
                     /translation="MVNATTILAQRDKALLDTDFPFLGIRQQGKVRDFYSYNGQRITI
                     TTDRQSAFDQVLGLIPFKGSVLNELAAWWFHKTRHIIPNHMIATPDPNVLISDDCQAV
                     PVEIIVRGYLSGATKTAIWSSYSQGERTIYGLEFPDGMVKNQKLPNPVITPTTHATGG
                     SHDERLTREQIIGKDMVPKEIYEEMERVSLELFRVGQLEALKHELILVDTKYEFGVVD
                     GKLLLIDEIHTPDSSRFWKADSYQERIEVGMEPENFDKEFLRLWYVERGYSGEGQPPH
                     MTPELVVQVAQRYMMLYEMLTGETFEAYEYPINGRIEAAIKRELNV"
     gene            16617..17726
                     /locus_tag="VF272_00930"
     CDS             16617..17726
                     /locus_tag="VF272_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7259477.1"
                     /translation="MSEPQKVTYQDSGVDYSKLDAMKRLAQEQAAGTASMLQQADMHE
                     VSASRGESAYVWDAGDHYGAMVQEGLGTKNLVADEVRKLTGKTHYDQIAQDVVGAITN
                     DLIVVGAKPLVVSAYWGLGDSAWLADEERAKDLAAGWANACKMAGAAWGGGETPAHTG
                     IVNPDTIDLGGSAVGIINPKERLVLGDKLDSGDAIVLIESSGIHANGLTLARKIADKL
                     PEGYATKLPSGTTYGEALLKPQHLYAQLVQDVFAADVDIHYMVHITGHGWRKLMRATQ
                     EFSYILTEVPPVPEELSFIAEHSGNDAGEMYGNFNMGAGFAIYVSADQAEKVMQAASD
                     NGMEAWIAGRVEAGAKKVVIEPIDVTFGGEALEVR"
     gene            17732..17980
                     /locus_tag="VF272_00935"
     CDS             17732..17980
                     /locus_tag="VF272_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259478.1"
                     /translation="MADYVQKTIAAYDHNFDKYQDATQCMLLQPEFERFVEMVRNVGD
                     FVLDAGCAYGRDTAAFMAEDIDVVLSKIDCNYSKNIVD"
     gene            18028..18579
                     /locus_tag="VF272_00940"
     CDS             18028..18579
                     /locus_tag="VF272_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259479.1"
                     /translation="MYDDRNKHLQDADRVRLMRWAALLAFIPTAFIVCAAIMLGVGQI
                     RGDVVKAHQNARLEFGHIVARGPYAGDAAFASYRLVSVRFRNWNREPVTDERMMPWDT
                     QIGQVVRVFVQPNGPAYMPRDLNRPGDNPYDPDTFARASSWFVFGGMLALLCGGLCWH
                     YIYRILVVRAIMLQQQRSPLATP"
     gene            complement(18727..19143)
                     /locus_tag="VF272_00945"
     CDS             complement(18727..19143)
                     /locus_tag="VF272_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259480.1"
                     /translation="MNSIIKIIDRFDIELNAEEMKKQGVEFGKQTVILPYSYSIGTSW
                     YLGEHLKKAASLGVKITIIDPKGEKHPGPIQKNTLPSGIDRMNINFNVQGLPVSRHGN
                     YTLLAEVVSEEDKVIGKGEYPFTVELVGRKIIPDEK"
     gene            complement(19196..19636)
                     /locus_tag="VF272_00950"
     CDS             complement(19196..19636)
                     /locus_tag="VF272_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7259481.1"
                     /translation="MDWKDVKQGLLEDEELRKAYEKVDLSHSIGKMIIDARIAKQMTQ
                     QKLAQLVGTQQPSIARIERGSYLPSLSFLQKIAEAFNTQLLPPRLEFLEKNKPAFSTT
                     TSTYFHSEPNREREFDRFNASWQGGHSSTNSRTFSIKELVQHGR"
     gene            complement(19652..19999)
                     /locus_tag="VF272_00955"
     CDS             complement(19652..19999)
                     /locus_tag="VF272_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7259482.1"
                     /translation="MIVTFWTSKSGRCPVADFIAKQHKAAAQRIMKDIDHLEQQGLKL
                     AISPNKLKPLQGYKKLYELKTDWGGVGYRIIFAIIDGKAWLLEAFKKKGNSTPQRHID
                     TALRRQVTIASAD"
     gene            complement(20094..23654)
                     /gene="purL"
                     /locus_tag="VF272_00960"
     CDS             complement(20094..23654)
                     /gene="purL"
                     /locus_tag="VF272_00960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX7259483.1"
                     /translation="MDMITRVERSRRFGFAKKLDEEDLAKIVDALHDRMTEQVYKQPL
                     TSFATNLKPEPVETVPILEEGIAALEQANKHYGLAMDEHDMQNYVSLFKKRLKRNPTT
                     VELFQLGQGNSEHSRHGFFRGKLVIDGKPVDKSLMQIVKAPYKHNPNNSLIAFHDDSS
                     AIRGGDIKTLVAQDPATASPFRIQDRVYHPTLTAETHNFPTGVAPYPGAATGTGGRIR
                     DNQSVGRGGLFAASGTGYTVGNLHIPGYDQPWEQDGHKQPPNLSSPLNILIEGSNGAS
                     DYGNCFGEPLILGHVQSFGAETEDGYRSWFKPIMYSEGAGQIDDRHTEKGAPEKGMQV
                     VQLGGPAYRIGMGGGAASSLMSGENAAELDFNAVQRGDAEMEQRMNRVMRACIELGDD
                     NPIISAHDLGAGGDSNALPEIVDPAGGRIDLRSIPVGDASLSVLEIWGNESQERNAFL
                     IWPEAINRFKQLCEREGAPYAVVGEVTGDGQLMVFDSQDDSTPVDLPLEPILGDLPPR
                     TIQLSTEAPKLKPLDLPSDLTVKDALNRVLRLVSVASKNWLIHKVDRHVTGLVAQQQA
                     VGPNQLPLSNYAVAAHSLFDQTGTALAQGERPRIGLISPAAQGRMSVAEALTNIMGAR
                     ITALEDIRASGNWMWAAKLPGEGAKLYEAATAMSELMIELGIAIDGGKDSLSMATKEK
                     DRTVKAPGQLVVAAYATMADVRTKVTPDLKAEGNDLIFIDLSDGHHRLGGSALAQTFQ
                     QIGNEAPDLKNTGLLKNTFHSIQALVADKLIVSLHDRSDGGLATTLLEMSFTGNFGIS
                     VAIKDKAHTIPLLFSEELGLVIETADREATEKLLHEYHVPHQIIGTVTKRGSHITISH
                     NDEKVLDEPMVDLREIWEDTSTQLEMLQSDPAHAKAEAKAIRNLIEPPKWQLSFSPAA
                     DRKALGNRNKPKVAVLRQTGTNGDREMAGALTAAGFDAWDVTMSDLQNRKASLVDFRG
                     VVFPGGFSFGDVLDSGKGWAGTIRFNKILADQFDEFYHRSDTFSLGVCNGAQLSALLG
                     WVPFKDIDQKEQPRFITNRSGRFESRFVTVEIQPSPAIMLKGMEGSRMGVWVAHGEGY
                     LHANSRLVNKLVEQNLAPIRFVDPHGGPTENYPFNPNGSPHGITSLVSTNGRHLAMMP
                     HPERLSNQLWQWPWMPDEWKDLDASPWLKLFQNAREWCEK"
BASE COUNT         5695 a         5500 c         6438 g         6033 t
ORIGIN      
        1 ccgatatgtt taccaccacc gatcgcaccg ttacggcgac gctgaaagat gggacccaaa
       61 acgttattta taaggacggc cagtttacgc tgtaggaggg tcggcagatg aacaaacgta
      121 atggtcttat cgctggcgca gtaattgcgg tggtagcaat ttttattggg ggttacgagc
      181 ttggtgcgcg cacggtggaa tcggccgaac ctccagccgc cgctactacg ccgcaaccgg
      241 ttaaggccga taagccggtt cgggctcacg cgcagctcta tattgataat catttaggat
      301 ttcaaatgga ggttccgttt gaatgggtga accggtatgc tccgattcag agcggcagca
      361 gcgtagggtt tgcgttcgaa aaagctacca gtccgcctgg cacaaccaag atggatttgg
      421 ttcaaatttt tacgattacc cgctatcctg cggtaaattt taatgaagcg gcgtgcgggt
      481 gcaaggaaat cggacgtaat aaaaatgctg ttttcggtta taaatttgat ggtcaaaacg
      541 tagccttggc gcaaccctta acagttgcga atattcaggc tgctatgcaa aacactattc
      601 cggaattttt taaaactctc gattagggct gaggctagca gtcttgacat aagagtgcta
      661 atttcctatg atatgagcag ttagcaaacc tgtaacgaga gtgcttatga ccccgcgcca
      721 gcaatatatt ttaagcgaaa tcgttgagac ctatgctcgg accgccgagc cggtaagttc
      781 aaacctgctt accgataaaa ttgaagtttc atcggcaacc atccgggccg acatggccga
      841 acttgagcgg ctgggctata ttttgcagcc acatacctcg gctggccgcg ttcctaccga
      901 tagtgggtat cgcatgtatg taaataacgt taaagagctg cacagcgacg ggcgcatcaa
      961 tcaggtactt gcccgccggg tgtcgagtgc cggtgaagcc gaccgcgcca ttaagcaagc
     1021 agctgaaagc ctggccgata tcactcaaaa tttggggctg gctaccttgt ctgaaggcct
     1081 ggtatttaca ggtctgcgta atttgttcgg ccagccggag tttagcggca ctaaggcttt
     1141 tgaagttgcc cgtttacttg atagcttgga tgagtggttg gccgaagcgg caccaagcgg
     1201 ccgagtgagc gtgtacattg gcacggaaaa tccgattggc cgcagcagtg gtgccaccct
     1261 ggtaattgcc cggtttgctt cgccgcatag cgattcatcc tatattggcg tacttggttc
     1321 gacccggcag aactacacca gggtgatggg tttggttgat tatgccggcc gctatttaga
     1381 ggagaccttg gtatgaccaa aaaaccgata tcaaaaaaac catcggtgca cgagctgcaa
     1441 gaacgggttg acgagttaac caatgacctg caacggcttc aagctgaatt tatcaactac
     1501 aagcgccgcg aggaagatgc caaatcggat attgccagtt ttgctaccgt acgggttgtg
     1561 aaagagttgc tgcccttgct ggataacatt gagcgggctt tggctcatcg tccggaagaa
     1621 cttaacgata atgactgggc tcagggcgtc gaacatgttg gtaagcagac gcttgaggta
     1681 ttgaaagtac tgggcgttga aaagattcag tcggtaggag agccgttcga ccacaaccgt
     1741 cacgaggcca taagtatgga ggacggtgaa ggcgataccg agatcgttat tgatgaagtt
     1801 caggcgggat ataccatggg cgatcaggtg attcgacacg ctatggtgaa agttggaaaa
     1861 ggaaatctac ggaataatga agaaggagaa actaatgggt aaaattatag gcattgattt
     1921 aggaacgact aactcggcta tggccgtaat ggaaggcgga cagccaacga ttattgctaa
     1981 tgccgaaggc ggccggacta cgccgtcgat tgtagcgatt aataaaaatg gtgaacgctt
     2041 agtcggccaa gccgctaaac gccaagccgt cattaacggt gaaaatacta tttttgaagt
     2101 gaagcgtttg attggacgca agtttgccga cgatgaggtt caaaaggatg tcgggcttat
     2161 gccctataag attgtcgacg ccaacggctc agtgaaagtt acgatgggcg acaaggatta
     2221 cagtcccgaa gaagtatcgg cgatgattct ttccaagctg aaagccgatg ccgaagcctt
     2281 taccggcggg ccggtaacag aagcggtaat cacagtcccc gcctatttta gcgatgctca
     2341 gcgccaagct accaaggatg ccggaaaaat tgccggtttg gaagtaaagc ggattattaa
     2401 tgagccgact gcggcggctt tagcctacgg actcgataaa aaagctgaag aaaaaattgt
     2461 tgtttacgac ttgggcggcg gcacctttga tgtttcaatt ctagaactgg gtgaaggcgt
     2521 cttcgaggtt aaaagcacca atggcgatac gcacttgggt ggcgccgact ttgacaatac
     2581 agtcatgacc ttcctgatag atgagtttaa aaaagaccaa ggtattgatt taaaggacga
     2641 taaggcggcg gttcagcgcc taaaggaagc ggcagagaag gcgaagattg agctttcaag
     2701 cgcccaggat accgaaatca atctgccgtt tatcaccgcc gatgccaccg ggccgaagca
     2761 cttcacctac catctcaccc gttccaaact gcaacagctg gtgcagggct tgattgataa
     2821 aaccgccggc ccttgtgaag cagctttgaa ggacgccggt ttaaccgctg ccgatattaa
     2881 cgaagttatt ttagtgggcg gaatgactcg catgccggcc gtagccgaaa aggtgcaggc
     2941 tatttttggg aaagagccgc taaagggggt gaatcccgat gaggtagtgg ccgttggtgc
     3001 tgctgtgcag ggaggcgtac tcgccggcga cgttaaggat gtcctgcttt tagatgtgac
     3061 tcccctttcc ttggggcttg aaaccatggg gagtgttatg accaaattga ttgagcgtaa
     3121 caccactgtg cctactagca aaagccaggt attctctacc gctgcagata gtcagaacca
     3181 ggtagaaatt aatgtgctcc aaggcgagcg tgagttcgct gccgacaata aaagtttggg
     3241 ccgtttcatt ctggatggca ttcctccggc tccgcgcggt gttccgcaaa ttgaggtcac
     3301 cttcaacatt gatgccaatg gtatcgtgaa cgtaaaagcg gttgataagg gcaccaacaa
     3361 agagcaacat attaccatta ctggcagcgg taacttaagc gatgaagaga ttaagaaagc
     3421 ccaggccgaa gccgaagttc atgctgaaga agataaaaag cgtaaagctg ttgtagaagc
     3481 ccgtaacaac ttagacaata cgatttatac cgcagagaaa accatcaagg atgccggcga
     3541 taaggcgaag gaagaagata aaaccgcgtt gcaaaccgct attgatgaag ccaaaaagca
     3601 taatgaaacc gatgacgcag cggttttaga aaaagccgct aaagatttaa gcgacaaaat
     3661 gcaggtggtt ggtgcagcca tgtatgccgc agacgacgct actggcagcg gggcgaccgc
     3721 tagcgacact gcagctagtg gcggtgcatc cgcttccgcc agtgccagcg ccgaaaccac
     3781 cactagcgag ccggttgaag gcgaggttgt tgatgagaaa aaagatggaa ctcccgaagc
     3841 tgggaagtag aaaagggtta caataggtgc cagtggctaa acgtgattat tatgaggttc
     3901 ttggtatttc caagtcggca tcggccgatg agattaagcg cgcctatcga aaactggcca
     3961 tgcagcacca tcccgataag catggtggcg atgacggccc attcaaagag ttgggtgagg
     4021 cttacgaagt tttaaaagac gaacaaaagc gggcagccta cgatcagttt ggccatgctg
     4081 ccggtgctca aaatgccggc ggacctaatc cattcggtgg cggcttcggt gcgcagggtt
     4141 ttgatttttc tcaatttcaa ggccaaggcg gcggttttgg agatattttc gatatgtttt
     4201 ttcaaggcca aggcggcgga agcaggcgac aatcagcccg cggcaccgat atggaaaccg
     4261 taattacgct tgattttaaa gaagccattt ttggcgccac gcgcaagctt tctctaacca
     4321 ccgatgaacg ctgtgaccat tgcgatggaa agggcggcga acccgggaca aagatcaaaa
     4381 cctgcgacac ctgcaagggc caaggtcagg taacgcgcgt acagcaaaca attttgggcg
     4441 ccatccagca aaccaccgtt tgtccgacgt gtcgcggcga gggtgaaatt cccgaagaaa
     4501 aatgcagtaa atgccgtggt gctggcgtga ttaagaaaac caaaaatatg gaagtaaaaa
     4561 ttccggctgg catcgataat gggcaaacga ttcgcctgga cggtaaaggc gcggcgcaca
     4621 aaaaagcccc gaacggcgat ctctatattc atgtccagat acgccccgat tccaaactgg
     4681 tacggcacgg tcaagacatt agttcaacta taaaggtgga tatggtgggg gcggcattag
     4741 gcagcgaggt tccggttcat acggtggatg gcgatgtgaa gttgaagatc ccagccggca
     4801 cgcaaagcgg caagatattt cgcttaagcg agcgcggcgt tccaggcata ggaggacgta
     4861 agcgcggaga tcacttagtg accgttaccg tcgaaactcc aagtaagctt tcagccaaac
     4921 agaaggaact tttagagcag ttcgcgagcg agggtcccaa aaaacgcaaa ttctggtagt
     4981 caggcgggct cctttaattc atcgtacgct gctttttgca ttgcttcggt aagcttatcc
     5041 caattcaaga tgaaaaggcg gtctggttgt gaggcttcat cgtcgtattg aatacgccct
     5101 ttttcttcaa atcccgacgc aagaagcacc tgctcagaac gtgtgttgcc gtcaataaca
     5161 tatgctgcca cggtaatttg cggctccagt acttgattgt tagttcgtgc tgcatcaagc
     5221 tggcttactg ctatgagcga ttgccggagc ccgctagcca tttgtttctc ctgtgctcct
     5281 tcaagtttgg tgtaagaaac ctcccaaata ttttcagccg gttgatcact aggaataatg
     5341 tgctgctcac gcagctggct aacacgcatt tcagcgtccg gattaagttt aacccatcct
     5401 tgcacctccc cgacttcgct tttatccaag gtaggatgct gctccgatcc ggcaactgca
     5461 aacagcaaat gatcgtcacc gtgttgcgaa aactcaatta actcctcgct tgttaaatga
     5521 acgtaatttt cggggcggct ttcaaaccat tgaattcgtt caggatcttg atcaagcgct
     5581 tggaggcgcg ccatgtcttc cgaccgctcg ggattgatgt ttctaatgct agatgtttgt
     5641 ccggctatac gccatgtttc gatttgttcc ggcggggggt gtggcgtgtt ttcaggcatg
     5701 aacacattac agttgaggtt ctattgtctg tcaatggtaa aagcggggcc tagcttatta
     5761 tgtagccaaa agggaaatgg ttgtacaata cacttatggt tactttatta tcaacaattc
     5821 cagccgcggt gcctgacttt gtttcagcgc taagcttaga tgccgctatc gtcgtcacaa
     5881 ctctattgct tgtcgtctat agcgtcgtac gcgggcatat ggcattaatt cgcgaagcta
     5941 ttagcattta tgtcgggctg gtgttatcgg ccagcttggg agaaccggtt tatgattatc
     6001 ttcataacgc cgcagccagc ctgccactga atgaaactgc tgttaaactc ttcttgctcc
     6061 tagctccagt tgtcgtttta cagattggga gacatcatcc gcgtggcggg cacaaacatc
     6121 acctaataat cacgtttgta cttgcggtga tgaccgggtt cttgcttatt tccagtgtgc
     6181 ttaaccagct tgaccaagtg actttaagcc atgctcttga aaattccaac ttagcggcct
     6241 ggatttatga gtttaagctt gtctggcttg gcgcggtgcc gttggcgatt gcggccagcg
     6301 cctttttacg gcccaaaggc caccattaac ggcttgacat acacacttat gctgtataac
     6361 ctttaagcat aagcacaatg cggaaattta aatgcctcgt aaaacaaaac atctttcgtt
     6421 aagcagtcaa gttcgcttta agttttcgca tgtactcatg tttgctgcgg cttttgcggc
     6481 gctgggaatt tacgctgtgc tgcatacctt cgccgctcct gttagcactc tttcggaatg
     6541 taccgtggtg ccgagtagcg caaaggcggg ggattctcac ctgacggtaa cggctccggt
     6601 tggagagctt ccagaaaatg cattggggga gaattaccgg gttaagctag aaacccccta
     6661 tataaatgag cagctagtgg atgccgtcta tgacgcagca aacgggattg ccatcttgaa
     6721 ctggaacggt ctcttagtta aaggcaagca ttccttttat ttcgtctatg taactggcca
     6781 caccggcaat agccaagttg taagagcgtc acgaacctgc agcatcaacg tcaattagtt
     6841 tttccgttga agcctagcaa aaggcaaaca gttacgctat agtaaaccag ttccgggccc
     6901 atagctcagt tggttagagc gagagccttt taagctctgc gtcctgggtt cgaatcccag
     6961 tgggccctcc aggtataata aaggacatgc ccaaacactt gtacctaata atcaatccgg
     7021 attccgggac cggtgatccg aaagcggtgc tttctgaggt ttcggcggag ttggatcaac
     7081 gcaccatcga gtatgtgctg aaaaaggtag agacacccca tgaagccttt acagcagctc
     7141 aagcgattga cctcaaaaaa ttcgatgccg ttggagtgta tggcggtgat ggtactatca
     7201 ttgccgtgtt taaggctttg tacaagcaaa aaacaccggt tttgataatg cctggcgggt
     7261 cggcaaatgt tttagccaaa gcactgaatt tgccggcgag cgcgggcgat tgccttgatc
     7321 tatttttagg taatttatat gtactacgtc attttggcgt ggcaatggct aatgatgcgc
     7381 cgttgatcct agatcttcat tttgggattt gggcagatag tattgaagca accaatcagc
     7441 gcactaagca acgatttggt cagctggcct atgccttttc cgcaatcaag caccttggtt
     7501 cggcatcccg attcaatttc caaatgacgc tcgacggcaa gcaggtggac gtttcggcct
     7561 atgcctgcat ggtggcgaat gaaggctacc atactattat aggcgtgcca tttttcccca
     7621 attcgcatac ccccggattt ttacaagtcg cggctgtaaa gcgagttaat caatgggcgt
     7681 ttctgcgctg gcttacgttg cggagactta gcatcccgac gtcgtcagta gtcgcgacct
     7741 ggcgggttaa agaagttgtt attcagcgcg cccctaagca gatgatgttt gacgatgata
     7801 ttgtgactgc caccctaccg cttactatta aaagttctcc ttacatggtt gcggtaatcg
     7861 cctctacacc tgataaaagc actttttggc gccgtttgca gatgtatgcc aaggtggaac
     7921 ccttccgacg gtatgatcat cttcgccgct ttttccgtgg cggaccatca ctgaagagca
     7981 gtcaaatagc acctaatctt tatgtcggag gacagtatcg tccgtgggcg attgcccggc
     8041 ttcgtcgctg gggagttacc ggcgttgtga atatgcgaaa cgtccgacgg ccatcaaact
     8101 ctcgtggctt ggaagtactt aatttgccga cgcccgatca ccagcctccg agcttagcat
     8161 cgctttataa aggcgtggaa tttatacaac atcatttaga tgccggcggc gctgtgtact
     8221 tgcactgcag gcttggagag ggcaggggac cgtcgatggc ggctgcttat cttatatcgc
     8281 agggaatgcg agccaatgat gcggtggcac acattgagcg ctttcgccgg ctcattcgac
     8341 cgaacgctaa gcaacttagg caactggctc gctttgaggc tgaactgaac cagaaaaaag
     8401 tatgaatgcc acaacctccg cggtgtctta aaatgtctat gcttattgac agttttctct
     8461 atcccaccta gtgtgaaggt agcttattga tgataaaaaa tggatattgc tccgcaaacg
     8521 aatagcacgc cctcggtttc gcccaagtgg ggaggtttgt taagttatgg tttaatcagt
     8581 gcttgcatcg caagctttat tagcatattc ctttttcttg ttgcccaggc aagttttaat
     8641 caagtatctg agctgaataa aacacttgcc cacgacctct catctcccat tataatgcta
     8701 acctacaccc tttggtggtc agctggcgct tttatattat ttgggacgat ctgtagtgct
     8761 tcttggtttt tacttctgcg cgttgatcca actactattc cttggaagcg ttcgataata
     8821 ataaatagca ttcagtggtt tgcactggct tttctcttat taaaggcagc cagtactata
     8881 ccagatgaat ggaagcagtt gatggcttca tcatgggcgg cattcttttt tgtgttcgca
     8941 tttcaaattg cgcctgcaat agccggtgca gccatcgtta ttgttatgtt ttggcgcaat
     9001 cgtttacgag ccactaggtc gccagcaccg ccgccaaccg aaattgaaca agccactcaa
     9061 atccctttac ctcaaaaaaa ctttttgagc agaactacgg cactccttgg ttttattttg
     9121 tttgcgccga tctttgttat tgcatcagta atcgcattat ccggcagcat attattcgtc
     9181 tatggattga taaccggtaa atttgatgaa ggaattagtc atctgattat ctttggatgg
     9241 acatattttg cctatggtgt cgcatcagct accggagtac ctgcaattgg gctggcaatg
     9301 tatttattcc gatcgaaagg cccactggtc tcggctggtg acaggaataa aagagcttta
     9361 ctcattggtt caccgattat tgtcggtctt ggcttaatag tgtggtttat gcagccacca
     9421 accaataccc agaaggaagc cgatgcttat caagacaaaa aggtgattgc atgctttaaa
     9481 atccctgatg gcggtacgta taatgcagtt tcgaacacgg taaaacatat tcctaatcgt
     9541 gaggtagcgc ctgtggttga tttgggctat tacagtaatt acgctgaaag gcacgatgat
     9601 gtactgggta ttaaattatc gccatcttct cggaaagatg cagaagaaga tgtaaaaatt
     9661 attaggcaaa actatctcga taaaatttcg ataattggca tctataacaa gaatgctatt
     9721 ccgccccata tgtgcggaat aaaataatac catcgtctga cattcctggt ttgttatact
     9781 aagcacgcat gttaggccat cgtaattttt cggcgctgcg gaattataat taccgcattt
     9841 atttcaccgg tatgagcatt tcactcatcg gtacctgggc gcaagtcatt gcgctttcgt
     9901 ggttagtact gcaaatgacc ggttccggga ctgctttagg gctgatccat gccgctcatt
     9961 ttctaccgtt gttaatttta ggcccttggg gcggattggt cgccgaccga cgtactaagc
    10021 ataagctttt acttgctacg caggcgtctt cggcaatttt atcgctgttg ctcggggtat
    10081 tagtggttac cggaaccgtt caattatgga tggtttttgt gattaccttg ggattagggc
    10141 tagtacgagt ggttgatcat cctacccgcc aagccttaat ttttgagatg gcagggaagg
    10201 atcatgtcca aaacgccatg gtattgcagg gtacttctgt aagcgttacg cgtatgctgg
    10261 gtccggcgat tgcaggtgta ctgattgcca ctcttgggat tggtatgtgt ttcattatta
    10321 acgccttttc ctatattccc atgctagtag ccatggcttt gctaaaagtt gatgaattgg
    10381 aagttgcacc gttaattgaa aaggctagcg ggcagctgcg cgagggtttc cgctatgtaa
    10441 tgaaaacgcc ggttattcgc aacaacctta ttatgatggc gattattgga acgctagctt
    10501 acgaatgggc ggtggtgctg ccgctgcttg cgagctttac cttccatggg gaggctgcgg
    10561 actatgcact gctaatgacc gccttgggta tcggcgccac cattggcggg ctgtataccg
    10621 cgcgctatcg cagcgttacc cagccgatga tctcactggc ggcattaatg cttggagcta
    10681 tggtggtgct ggtggcattg gcgccgagct tactgtttgc ggtagtggct atgggcttgg
    10741 ttggcttttt cgcggttcag tttgattcat taaatagcag tatcctgcag cttaccagct
    10801 ccccggaaat gcgcggacga gttatggcac tgtggggcgt tgccttctta ggatcttcgc
    10861 caatcggcgg cccgcttatt ggatggatct gtcagctagc cggaccgaga accggattat
    10921 tgataggtgc ggtggccgcc atggccgccg ctactttcgg ctatgtgact acgaggcggt
    10981 caagcgttat actgaagaca tgagattact ggcgctgttc aagatttatg tctgaacgaa
    11041 agccccatag acctaaacgt atttcagccc ggcgggtggt tattacctcc tttgttgtcg
    11101 acttaggcga tattatcctg aatgttatta ccgccgtgct taccggtagc gtagttgtgc
    11161 ttactgaagc tttgcaaggc gctgccgatc tgttaacctc ggccctgctg tgggtgggtg
    11221 tgcagcggtc gaagcgccaa gcgacgcgcg ttcaccgctt tggttatggt aaggagctat
    11281 tcttctggat tttaatggct ggggtttcga tgttcattat taccgccggg ttctccttct
    11341 actttggttt ccagcggatc ctgcatccgc atccgattca aaatattgaa ttggcgtacg
    11401 tggttctgac tatcggattt gccaccaacc tgtatgcttt aagtttaagt ttgcggcgtt
    11461 tgggcgtgag tttacggcat aagctcagcc atatccgccg acacattcgg aattcagcct
    11521 taattgaaac caaggcgacc accatcttag accttatggg tgccagcgcg gccatgctgg
    11581 gtctgatttc cttggttatc tacggcatta ctggtgaatc ccgattcgat ggattgggcg
    11641 cgatggtagt tggattgagc tgcgcagtct ttgctttgct gcttattttt gaagtaaaga
    11701 atttcattgt cgggcgcagc gcctcaccgg aggtcgaatc ccaaattaaa gcagtagcta
    11761 ccaacgtagt cggagtccat agcgtgcttg atttgcggac gatgcaaatg ggctcggagc
    11821 gggttatggt gaacatggaa gttcacttag accataggct aagaacccaa gaaattgagc
    11881 agcttatgga tgacctgaaa gaagcggtaa agaaggatgt gcccgaagtg cagcatattc
    11941 aagttgaagt cgagacaccg cgtcgcaagc cggtaaggcc ttccaatgat taaactggct
    12001 gtgttggttt ctggtaccgg atcgatttta gaagctatgt tgcagcagga agtgccggta
    12061 gctttggtgg tggccgaccg cccctgccgc ggattggagt tggccgaaca ggccggtatt
    12121 cccacggcac taattgaccg cacggctttt ggctttagcg gaccgggttc agagtgggac
    12181 cgcactggct ttacccaggc gattattgag aagttgaagg agcaggacat tggcctggta
    12241 gcaatggccg gcttcatgac tattttagat ccagtgatat ttgaggcttt tcctactcgc
    12301 attctcaaca cccatccttc gctgctcccg gcgtttaaag gagatgctgc ggtgcaagat
    12361 gcattagacg ccggcgctag cgtaaccgga tgtactatcc attatgcatc cgctgatttg
    12421 gacggcggac cgatcatcgt gcagcgcgag gtagaaattc tcccaggcga tacaagggaa
    12481 tcattacacg aacggattaa aactatggag cggaccatgt atcccgaagt tgttaagaaa
    12541 ttgttggctg aagatgatta acaaagcttt acacaattca tatttatggt ataattaaag
    12601 tatgcagggc gacttttcat cttataattc accattaggg taccgatacg catctcctga
    12661 aatgcgtgag ctgtttagcg aagcaaccaa atacaagctg tggcggcgcg tttgggtagc
    12721 tcttgccagg gctgaacatc aagccggact tatcaacgat gaagaactcg ccgacttgga
    12781 ggccaatcaa gacactattg atattgcgcg catcctggaa attgagcgcg ataccaagca
    12841 cgacgtggta gcggccattc gcgagtacgc cgaaaaagcc aaaatcggcg gcggcaagat
    12901 tcatcttggc gcgaccagca tggacatcaa tgataacgcc gatgccatgc gggtaaagca
    12961 ggcccttgaa cttatagaag accggctgaa agccctattg aaggttgtgg cggcgcaggc
    13021 cgataggcat gctgagacgc cgtgcttagg atatacccat ctgcagccgg ccgaacctac
    13081 cactattggc taccgcttgg cattttacgg tgatgattta ttaagtgacc ttcagcgtat
    13141 tcaaggactt cactaccggt ttgtcggtaa gggcatgaag ggggcaattg gcacacgggc
    13201 tagctatacc gccattttag aaggtaccga tatgaccgcc gcccagctcg atgaaaaggt
    13261 aatggccgaa cttcacctac gcccggcctt aattgctacc caggtgctta cccgcaagca
    13321 agactatgac attctttcag ctctggccgg cattggcgga agcctcgcta aaatgggggc
    13381 tgatattcgt ttgctgcagt caccgggatt cggcgaatgg tcggaatcgt tttcagataa
    13441 acaagttggt tcttcggcaa tgccgtttaa aaagaacccg attacggcgg aaaagatttg
    13501 ttcgttggct cgccagctgg ctcagctgcc tgccgtggca ctcggcaatg cctcgcattc
    13561 atacttagag cgtacgcttg atgattcggc caatcgccga atgactacac cggaagcctt
    13621 cttgctggcc gacgaaattt taattaccgc cacaaaactg attgaaggct taactatcaa
    13681 tggagataga gtggctgaaa acctagctca gtttgggccg ttcgctgcta ccgaagtttt
    13741 aattattgag gcggttaaac aaggcgccga ccgtcaggct atgcatgagc tgctgcgcac
    13801 ggttgcattg gaggcttggc aacagccgca gccgggtaag gccatggttg aactgttaaa
    13861 agacaacgca gagcttggca agcacgttac ctcggaacgc ctcgatgagc tgttagacgt
    13921 tcgaactcat ctgggagatg ctcccgaccg ggtcaagcag ctcgtggcac ttattcacaa
    13981 agaggttgaa ggttgaacca ccgcttaggg atagttggcg ggggtcaact cgggcgaatg
    14041 gtggcattcg acgctaaaag actggggttc gcggtaaccg tgcttgaccc ggaacccaac
    14101 agtccggctg ggcaagtggc agacgagcat atcgtagcag cctatgacga tgatgccgcg
    14161 gtacgaaggt tggccaagtc tgtcgatttc atcacctatg atattgaatc agctggcaag
    14221 ggggttttag ccgagttggc cgaaaatggt aagcaagtca atccgtctcc atggacgttg
    14281 aatatgatcc gagacaaatt gggccagcac cgttggctac gggaggtcgg tattccggta
    14341 gcagagttta tggaggttga aaccgacgag gacatcaagc aggcgggaga aacgtttggc
    14401 tatcccttgg tgttgaaggc tcgctttgac gcctatgatg gtcgaggcaa tgcaattata
    14461 aaatcctctg atgaaatcgc ctcagtgaaa gaaaagctgg ctggccgcga gctttatgtc
    14521 gagcagactg ttccatttat taaagagctc gcggtcatag ctgttcgcgg tatggacggt
    14581 gctattgcgg tttacgacgc agttgaaacc attcatgaaa ataatatctg ccacatggta
    14641 tacgcaccgg cgcgggtaga gaaaacagtc gtacataaag ctcgggaact ggccgagcaa
    14701 gtggtaggcc agcttaaagg ggcgggcgtg ttcgcggtgg agatgtttat aagcgcagac
    14761 ggtaaagtaa ttgtaaacga gatagctcct cgggtgcata atagtggcca tctaactatt
    14821 gaggcggcag tgacctcgca gtttgagcaa catgtacggg cggtgactgg actgcccctt
    14881 ggcgatacaa gtctaaaagt accagcggcg gtgatgatta atatactggg tgagcgctcg
    14941 ggtccagcta atcccgaagg tttagctgag gctttggcaa ttccgggtgt cagcgttcat
    15001 atttatggta aagctaaaac gtggcctcag cgtaaaatgg ggcatatcac ggcaattgct
    15061 gatacccttg aagcagcgga acaaaaggcg cttgaagcca gaaaggcggt aacaatatga
    15121 gcgaaagaga gcagccacaa gttggtatta ttatggggtc ggattcagac ttgccagtga
    15181 tgaaacaggc ggcagaggtc ttggagtcgt ttggtatttc ggcagaagtc actgtagtgt
    15241 cggcccaccg tactccggag cggctgtggg agtatgcaac tcaagcccgg agtcgggatt
    15301 ttaaggttat cattgccggc gccggcgggg cggctcattt gcctggtatg gtcgcttcta
    15361 tgaccgagtt gcccgtgatt ggcgtgccga ttcaaaccaa gaccatgaaa gggatcgatt
    15421 cactcctctc catagcccaa atgcctcctg gtattccggt ggcaaccatg gcggttaatg
    15481 gcgccaagaa cgccgggtta cttgctgttc ggatgctggg gctgggcgac tcagctattt
    15541 tggactccat gcacgattat caaaaatcac tggaagcaga aataaagatc aaggctgaga
    15601 agctggaagc ggttggttac gaggattatc tcaaggaaaa gggggagtag gttggttaat
    15661 gccactacca ttttggcgca aagggacaaa gccctgctcg atactgattt ccccttttta
    15721 ggtatccggc aacaggggaa agtgcgcgat ttttattcat acaatggtca gcgcattacc
    15781 attaccaccg atcgccaatc ggccttcgat caagtgcttg gcctgattcc gtttaaaggc
    15841 agcgtcttaa atgaactggc agcctggtgg tttcataaaa cccggcacat tattccgaac
    15901 cacatgatcg ccacgcccga ccccaacgtt ctgatttccg atgactgtca ggcggtaccg
    15961 gtagaaatta tcgtacgtgg ttacttgagc ggtgctacca aaactgcaat ttggagctcc
    16021 tacagtcagg gcgagcgcac catttatgga ctggaatttc ctgatggtat ggtaaagaat
    16081 cagaagttgc caaacccagt aattacgcct actactcacg ctactggcgg cagtcatgat
    16141 gagcggttaa cccgggaaca gattattggc aaggatatgg ttcccaagga aatatatgaa
    16201 gagatggagc gagtcagctt ggagttgttt agggtgggcc agctggaagc ccttaagcat
    16261 gaactcatac tggttgacac taagtatgag tttggcgtgg ttgatgggaa gttgttgcta
    16321 attgatgaaa ttcacacgcc cgactcgtca cggttctgga aggccgatag ctaccaggag
    16381 cgaatagagg ttggcatgga gccggaaaat tttgataagg aatttttacg gctgtggtat
    16441 gtcgagcggg gctatagcgg tgagggacag ccgccccata tgacaccgga actggttgtc
    16501 caggtggccc agcgctacat gatgctgtat gaaatgttga ccggtgagac ctttgaagcg
    16561 tacgagtatc cgattaatgg tcggattgaa gcagctatta agcgggagct aaacgtgtga
    16621 gtgaaccgca gaaagtcacc taccaagaca gcggcgtcga ttactccaag cttgatgcga
    16681 tgaaacgttt ggctcaagaa caggccgccg gtactgcttc gatgctacaa caagcggata
    16741 tgcacgaagt ctccgccagc aggggggagt cggcctacgt gtgggatgct ggcgaccatt
    16801 acggcgcaat ggttcaagag ggtttgggta ccaaaaatct ggtggccgat gaggtgcgca
    16861 aacttactgg taaaactcat tacgatcaga ttgcccaaga tgtagtagga gcgattacca
    16921 atgatcttat tgtagtcggt gctaaaccgt tggtggtttc tgcctattgg ggcttaggtg
    16981 attcagcctg gcttgccgat gaagagcgtg ccaaagatct ggcagccggt tgggccaatg
    17041 cctgcaagat ggctggagcg gcttggggag ggggagagac cccggcgcat accggcatcg
    17101 tgaatccgga taccattgat ctcggcggtt cggccgtcgg cataattaac ccgaaagagc
    17161 ggttggtcct tggcgataag ctggatagcg gcgacgcaat cgttttaatt gaaagcagcg
    17221 gtattcatgc caacggttta accttggctc gcaagattgc tgataaattg cccgagggtt
    17281 acgccaccaa actccccagc ggcacaacct atggcgaagc cttgttaaag ccccagcatt
    17341 tatatgccca gttggtccaa gacgtttttg ctgccgatgt tgacattcat tacatggtgc
    17401 acattaccgg ccacggctgg cgcaaattaa tgcgggcaac ccaggagttt agctacatcc
    17461 ttaccgaggt gccgccggtg ccggaggaac tctcttttat tgctgagcat tccggcaatg
    17521 atgccggaga gatgtatggt aacttcaaca tgggagccgg ttttgcgatt tatgtttcgg
    17581 ccgatcaggc tgaaaaagtt atgcaggcgg ccagcgacaa tggcatggaa gcttggattg
    17641 ccggccgggt agaagctggt gcgaagaaag ttgtgattga gccgattgat gttaccttcg
    17701 gtggagaagc gctggaagtc agataactag tatggctgac tacgtacaaa agaccatagc
    17761 agcctacgat cataacttcg ataaatacca agacgccact caatgcatgc tgcttcagcc
    17821 tgaattcgaa aggtttgtcg aaatggttcg aaacgttggg gattttgtct tagacgcggg
    17881 ctgtgcctat ggtagggata cagcggcttt catggcggaa gatatagacg tcgttctgag
    17941 taaaattgat tgcaattata gcaaaaacat tgtagactga catgcacgtt gcggataagc
    18001 cgcgcgattg agaataaagg agtcggcgtg tacgacgacc ggaataagca tcttcaggac
    18061 gctgatcgag tgcggttgat gcgctgggcg gcactgctcg cgttcatccc gaccgctttt
    18121 atcgtctgtg cggcgatcat gttgggagtt ggacagatcc gaggcgacgt tgtcaaggct
    18181 catcagaacg ctcgccttga gttcggccac atcgtcgctc gcgggcccta cgccggtgac
    18241 gcagcgtttg cctcgtaccg cctggtgagc gttcgttttc gcaactggaa tcgcgaaccg
    18301 gtcaccgacg agcggatgat gccgtgggat actcagatag ggcaggtggt tcgggtgttc
    18361 gttcagccca acggcccggc ctacatgcca cgggacctga accgtccggg agacaacccg
    18421 tacgacccgg acacgtttgc gcgggcttct tcctggttcg tcttcggggg aatgcttgcc
    18481 ttgctatgtg gcggtctctg ctggcactac atctatcgca tcctggtggt gcgtgcgatc
    18541 atgctccagc agcagcgaag cccgctggca acaccctaaa tccacccgcg ctccggcaca
    18601 cgcgaaggcg tcacgacatc gtgattgcat gcggtgccgg ggcgccttcg cgttattact
    18661 caaaatctaa aagcattctt atgcttgcga gaaaaatata ggggctagcc gtaaaaaatg
    18721 tattccttat ttttcgtcgg ggataatttt gcgtccaact agttcaacgg tgaatgggta
    18781 ttccccttta ccaataacct tatcttcctc agaaacgacc tcggcaagga gggtgtagtt
    18841 tccgtgtctg ctgaccggta atccttgaac attaaagttg atgttcatcc tatcaatccc
    18901 cgatgggagc gtattcttct ggattggtcc tggatgtttt tcgccttttg gatcaatgat
    18961 ggtaatttta acccctaaag aagcggcttt ttttaagtgt tcccccagat accatgatgt
    19021 gccaatggaa tatgaatagg ggagaataac ggtttgcttt ccaaactcga ctccctgttt
    19081 cttcatttct tcggcgttga gttcaatatc aaatctatca attatcttaa taattgaatt
    19141 cattctatcg tttgcatcgg tggtgatttc tctagcaaga actacgtaaa ggcatttaac
    19201 gtccatgctg caccaactcc tttatgctga acgttcggct atttgtggat gagtgacctc
    19261 cctgccaact cgcgttaaaa cgatcaaact ctcgttcacg attgggctcg gagtgaaaat
    19321 aggtacttgt tgtggtgctg aaggcaggtt tgtttttttc caagaattcc aggcgaggtg
    19381 ggagcagctg ggtattaaat gcctcggcta tcttctgtaa gaacgagagg ctcgggaggt
    19441 agctccccct ctcgatgcga gcaatgcttg gctgttgggt tccaacgagt tgagccagtt
    19501 tctgctgagt catttgtttt gcaatgcggg catcgataat cattttccca atactgtggg
    19561 aaagatcgac tttttcatat gcttttcgaa gttcttcgtc ctcgagtagg ccttgtttta
    19621 cgtctttcca atccataatt aaatctcctt tttaatcagc gcttgcaatc gttacttgtc
    19681 ttcgcaatgc agtatcgata tgtcgctgag gcgtgctgtt ccctttcttt ttaaatgcct
    19741 ctagaagcca ggccttaccg tcaataattg caaaaattat ccgataacct accccgcccc
    19801 agtcagtttt aagctcatat aatttcttgt aaccctgcag tggctttagc ttatttggac
    19861 taatagctag ttttagtcct tgttgctcaa gatgatcaat gtccttcata atgcgctgtg
    19921 ccgctgcttt atgttgcttg gctataaaat ctgcaacggg gcatctgcct gattttgatg
    19981 tccagaatgt tacaatcatg caccctcctt gccatagccg ccgtctcctt tataacaata
    20041 ttgttatatt atcgtgaagg gtgtaatgat gtcaaggggc acagtcggtc gatttacttt
    20101 tcgcaccact cgcgggcgtt ttggaatagt ttgagccacg ggctggcgtc gagatccttc
    20161 cattcatcgg gcatccatgg ccactgccac agttgattcg acaggcgttc ggggtgcggc
    20221 atcatggcga ggtgccggcc gttggttgag accaatgatg taataccgtg cgggctgcca
    20281 ttggggttaa aggggtagtt ttcagtcggt ccaccgtggg gatctacaaa tcggatcgga
    20341 gccagatttt gctcaactaa tttgttcact aacctggaat tagcgtgtaa gtagccttca
    20401 ccatgagcta cccagacgcc catccgcgag ccttccatcc cctttaacat gatggccgga
    20461 ctgggctgaa tttcaaccgt aacgaagcgc gattcaaacc gtccggagcg gttggtaata
    20521 aaccgcggct gctctttttg gtcgatatct ttaaaaggaa cccagccaag cagggcggaa
    20581 agttgggcgc cgttacaaac tcctaaagag aatgtgtcgg agcgatgata aaactcgtcg
    20641 aactggtcgg ccaggatttt gttaaagcga atggtgccgg cccagccttt gccagaatcc
    20701 agcacatcgc cgaatgagaa gccgccgggg aagaccacgc cgcggaaatc gaccaggctg
    20761 gctttgcggt tttgaaggtc ggacatggta acgtcccagg cgtcaaaccc cgcggcggtg
    20821 agagcaccgg ccatttccct atcgccattg gtgccggttt ggcgtaagac ggcgaccttc
    20881 ggtttattgc gattgcctaa agctttgcga tcggcagcgg gggagaagct cagctgccat
    20941 ttgggcggtt cgattaaatt acggattgcc ttagcttcgg cctttgcatg ggccgggtcg
    21001 gactgcagca tttctagttg ggtgctggtg tcttcccaaa tttcacgcag atcaaccatt
    21061 ggctcgtcga gcactttttc atcattgtgg ctaatggtaa tgtggctgcc gcgtttggtg
    21121 acggtaccga taatttggtg ggggacgtga tattcgtgga gaagtttttc agttgcttcg
    21181 cgatcggcag tttcaataac caggccgagt tcttcgctaa agagcagggg aatagtatga
    21241 gccttatcct tgattgcgac actgatgccg aagttaccgg taaaggacat ttcaaggagg
    21301 gtggtggcca acccgccgtc actgcgatcg tggaggctta cgatgagttt gtcggcaacg
    21361 agagcttgaa ttgagtgaaa ggtattttta agaagcccgg tatttttaag gtcgggcgcc
    21421 tcattgccaa tttgttggaa ggtctgagct aaagctgatc cgccgagacg atgatgtccg
    21481 tcggaaagat caatgaagat caggtcgttg ccttcagctt taagatcggg tgtaactttg
    21541 gtgcgaacat ctgccatagt ggcgtaggcg gcgacgacca actggccggg agctttcacg
    21601 gtgcggtctt tttctttggt agccatagaa agactgtctt tgccaccgtc gatcgcaatg
    21661 cccagctcaa tcatcagctc gctcatggcg gtggcggcct catagagctt ggcgccttcg
    21721 ccgggcaact tggctgccca catccagttg ccgctggcgc gaatatcctc aagcgcggta
    21781 atacgggccc ccattatatt ggtgagggct tcggctacac tcatccggcc ttgggcggca
    21841 ggggaaataa gtccaatacg cggacgttcg ccttgcgcca gcgcggtgcc ggtttggtcg
    21901 aacaaactat gggcggctac ggcgtagttg gaaaggggga gctggttagg gccaacagct
    21961 tgctgttggg cgaccaggcc ggtaacgtgg cgatcaactt tgtgaatcaa ccagtttttt
    22021 gaggcgacgg agacgaggcg cagaacgcga ttaagggcgt ctttgacagt gaggtcgctc
    22081 ggcaggtcga gtggcttgag ttttggtgct tcagtgctaa gttgaatggt tcgcggggga
    22141 aggtcgccca ggattggttc cagggggagg tcgaccggtg tagaatcatc ctgactgtcg
    22201 aataccatga gttggccgtc gccggtaacc tcgccgacca cggcgtaggg agcaccttca
    22261 cgctcgcata attgcttgaa gcggttgata gcttccggcc aaattaagaa tgcattacgc
    22321 tcttgcgatt cgttgcccca aatttccagg accgaaaggc tggcgtcgcc taccggtatt
    22381 gatcgcaggt cgatacggcc gccggccggg tcaacaattt ctggcagagc gttggagtcg
    22441 ccgccagcgc caagatcatg ggcggaaata atcggattgt catcgccgag ctcaatgcaa
    22501 gctcgcatta cgcggttcat tctctgctcc atttcggcat cgccgcgctg aacggcattg
    22561 aaatcgagct cagcggcatt ttccccgctc atgagtgaac tggccgcgcc gcctcccatc
    22621 ccaatgcgat aagccggtcc gcccagctga accacctgca tgcctttttc cggtgcacct
    22681 ttttcagtgt ggcggtcgtc gatttggccg gcaccttcgc tatacatgat tggcttgaac
    22741 cacgagcggt agccgtcttc agtttccgct ccaaacgatt gcacgtggcc aagaatcagc
    22801 ggttcgccaa aacagttgcc gtaatcggag gcgccattgg agccttcaat taaaatattg
    22861 agcggggatg atagattggg gggctgttta tggccgtcct gctcccaggg ctgatcgtag
    22921 ccaggaatat ggagattgcc gacggtgtaa ccggtgccgc tggcagcaaa caatccaccg
    22981 cgccccacgc tttggttgtc gcggatgcga cctccggtgc cggtggcagc gcccggatag
    23041 ggggcaacac cggtggggaa gttatgggtt tcggcggtga gagtggggtg gtaaacccga
    23101 tcttgaatcc tgaacggact ggccgttgcg ggatcttgcg caaccagagt tttaatgtcg
    23161 ccgccgcgaa tggccgagct gtcatcatga aaagcgatca atgaattatt cggattatgc
    23221 ttgtagggtg ctttgacgat ttgcattaag gatttatcaa ccggttttcc atcaataacc
    23281 agcttgccgc ggaaaaaacc gtggcgggaa tgttctgagt tgccttgccc aagctggaac
    23341 agttcaacgg tagtgggatt gcgcttcagt cgctttttaa aaaggcttac gtagttttgc
    23401 atgtcatgct catccatcgc cagaccgtaa tgcttgttag cctgctctaa ggcagcgatg
    23461 ccttcttcca gaatcggtac ggtttctacc ggttcgggtt tgagattagt tgcaaaactc
    23521 gtgagcggtt gtttatagac ttgctcggtc atccgatcat ggagggcatc aacgatttta
    23581 gcgaggtctt cttcgtccag ttttttagcg aaaccaaatc ggcgcgagcg ctccactcga
    23641 gtgatcatat ccaggcctac ggcgtg
//
LOCUS       DASVGB010000033         9032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_814585, whole genome shotgun sequence.
ACCESSION   DASVGB010000033 DASVGB010000000
VERSION     DASVGB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814585"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VF272_00965"
     CDS             complement(<1..616)
                     /locus_tag="VF272_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEX7259484.1"
                     /translation="MKCSALALIVLMVASIVVAALPVRSIRAAGSVQLSLGPAAHNVS
                     KGATFTMTINLATVGEAVDTVSAELTYPSSRLACVSVANSPDFTLSPQPDVCSGGSIV
                     IQRGRTSGHTSNGVIATVTFQTLMSGDAQVTVNNTSQVLSGGEEVDYTSASADYYVVN
                     GLISYTWALSGQGGYTDASRTTPRDLSTMMAGQSAWLVVRATNTG"
     gene            719..1372
                     /locus_tag="VF272_00970"
     CDS             719..1372
                     /locus_tag="VF272_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259485.1"
                     /translation="MIEAKKSRPYWHVDAKWICGLLLVVMLGAGLFAVGLYRLTERSS
                     AQALTGAIATTFFINPNADVSANEVRHKLQPLYDQGLGAIAETASPDPKKQAEITKQL
                     RPLALLTNDAHSLVYKIILGISIATLILTGGLIFFSRRFGKLVSVGVVLLVLALPGTL
                     LFLTFQSGGATNGMHIGSIGPPILTLIASAFLPGYAIALAMAAGLLVLSGVLKLIRR"
     gene            complement(1388..1678)
                     /locus_tag="VF272_00975"
     CDS             complement(1388..1678)
                     /locus_tag="VF272_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX7259486.1"
                     /translation="MSKYHHIILFRLKDGISGEQNQHALALLNQAGQGCQGLERWEVH
                     ESVDGRKGHIIILESLFASEEDFQNFHNSEKHNEATSFLCEVADWWVGDYVE"
     gene            1976..3442
                     /locus_tag="VF272_00980"
     CDS             1976..3442
                     /locus_tag="VF272_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7259487.1"
                     /translation="MQTTLLMRFFSFFILASAWMVAMPGEAHAVPPIVFPVIGPASYS
                     NDFNAPRTNGIHGATDIFAPKGRLLVSPVNGTITSVIVPQASWGYSVTVRDTEGYSFR
                     FIHMNNDTPGTDDGQGGPMNAYAADMKTGNPVVAGQLLGYLGDSGNAETTAPHLHFEI
                     FAPDGSLINPYDSLNRASRIPGPVHYPALPNEVLPYPGLTGPMKIAMGNFEGSSTTSE
                     MITGAGSGAPPHVIAARTDGTVLASFYAYSQGFRGGIDVAAGDIDGDGIDEIITGTGP
                     GAGPHVMVYKLNGTPVASFYAFNPSFKGGISVSAGDLDGDGNDEIVVGAGPGAGPHVI
                     AYKADGSTVVASFYAYAQGFKGGVDVAAGDVEGTTDAEIITGAGPGAPPHVMVYQPDG
                     TSLGSFYVYDQGNRSGVRVSAGNIRTSSTKDEILTAPEALPGGPLIKVVTYDGTTISQ
                     FNFMESWWLGYHDIGAGYDTSRAATGGNRRASVRVGPS"
     gene            3481..3870
                     /locus_tag="VF272_00985"
     CDS             3481..3870
                     /locus_tag="VF272_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7259488.1"
                     /translation="MNEDIQTYNNGQEETSKALCELLIHEIRLALPHAESKIWHGAPV
                     WFLSGNPIVGYDVLKDCVRLLFWSGQSFNEPGLKKEGSFKAAEARYTDTSQVSKVDLA
                     RWLKKSIEIQWDYKNIVKRRGVLERLR"
     gene            3883..4134
                     /locus_tag="VF272_00990"
     CDS             3883..4134
                     /locus_tag="VF272_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX7259489.1"
                     /translation="MITKEISGGVAHEMPADLRKALTSTSTALAAWEDITPLARNEWM
                     CWVEEAKKTQTRAHRIERTRTELMEGKRRPCCWAGCNHR"
     gene            4360..5037
                     /locus_tag="VF272_00995"
     CDS             4360..5037
                     /locus_tag="VF272_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259490.1"
                     /translation="MHSIKRFAKDYSLPLAATVLFGLLITGSVVLRMSQRASLADWLD
                     AATNDGKYSSTLLSQDKPEEVTRGNDTPQQLTQTPEGSPTSFAISPNNQPVANPSTPP
                     GGGTPLSPLFSVSIAYFQKGETTLECSTPKPKPKTCSKQYVFNGGVRTQNGPGNVTYS
                     WRSSIQSLTASGSFTASRGEALAPLQKIVTMPCDTVSDFSLQLVVLSPSFVQSTVFNT
                     NHNCNEI"
     gene            5050..6972
                     /locus_tag="VF272_01000"
     CDS             5050..6972
                     /locus_tag="VF272_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7259491.1"
                     /translation="MHKEHPSNLQRHFIMWVLLPPFGVFLALSILGLWQLEVMLHRQA
                     IGDLQRASTATAANVNQQFSLHETVLKQMGTELFVIKNEYRTNRKNLEDNYNACRAYL
                     LKYATPKLAPNGTCQPFLGGLGTTGNLVALEDEHVRLVEKLIQNQNQRINERLAAFKE
                     FFPETLALLITDGSKQTVSSALSGAFQGSTLHFQADAEAALSKPIRGKIAEVEGFKMA
                     IFGFPIGDGSVIAAYDLQNKNFVRRTWSNAPVDKTRELVVLLDSSGKPAYPQLRDGKQ
                     FAGKINDLRHKPFVHIKLDNVDHTVVGTPIAGSDWMAVVASPTAAIHAASRDAQLAGL
                     MLIGLFMIGFLWVGTFFIQRTLRNIINLALGAQIFGSGKLDHTITLKHGDGEFLQLAD
                     TMNGMARRIAAAEQAIDEKNKEFISIATHELRSPLTAMIINLSAFQEMHVKNLDDEAR
                     HYADEAYASTIRLRDLVNDMLSMARLESGNPEFTIDEVDIDGLVKNIVDMMSGVAAKS
                     KVELSYQDKSVSKVMADEQYLRVILNNFVSNAIKYNRPGGKVVISHELKGDKLVTIVA
                     DSGLGIPDPQKAHIFEKFFRIRDTDRKDIPGTGLGMYITKQYIEQMHGEVWFESTHGK
                     GTTFYFTLPVAKKPPS"
     gene            complement(7118..7462)
                     /locus_tag="VF272_01005"
     CDS             complement(7118..7462)
                     /locus_tag="VF272_01005"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX7259492.1"
                     /translation="MEDRLNKIQSNPASASIYAKLLDLLNKFPEYQAESKKTSLHIIN
                     KKAFLGVHPRKEGLLLNIVLSEPIKSSRIKKTERVSANRYHNEIELRSVDELDDEVAG
                     WIKAAYVRTEAS"
     gene            complement(7509..>9032)
                     /locus_tag="VF272_01010"
     CDS             complement(7509..>9032)
                     /locus_tag="VF272_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7259493.1"
                     /translation="EPTVEQTIEILRGLRDRYEAHHKVKITDEAIVAAAGLSDRYIAN
                     RFLPDKAIDLIDQAASRVRIGADTYTQEMTELDNQIAWQEREREYASNHRQFDEAKKI
                     EKQLGDLGKKKEKLSDTWRAQKGVTSKEVLSAHVAQVVSRITGIPVSELTQEEREKLL
                     KMEEHLHERVIGQEEAITAVSDAIRMSRAGLSEGSRPIATLMFLGPTGVGKTELAKAL
                     AWQVFGDEDAIIRIDMSEYMERHTVSRLIGAPPGYVGYDEGGQLTERVRRRPYSVLLL
                     DEIEKAHPDVHNILLQVFDDGRLTDGKGRVVDFSNTIIISTSNLGAQIIQDSLTAGQS
                     SQARQNDTVEKKSYAALKQELMEVVRLHFRPEFINRIDEIIVFRSLTKDQIKEIVGLQ
                     LERVKRMARGQAIELDFDLSTINHLADVGYVPEYGARELRRQIKSEIENRLAKEILSG
                     HIGEGSEVRIKYDEHEHNMIFEPLSDQSRKERPIKRELSFREKLDAETDKEQEDDRK"
BASE COUNT         2261 a         2285 c         2154 g         2332 t
ORIGIN      
        1 tgcctgtatt ggtagcacgc actaccagcc aagccgattg tccggccatc attgttgata
       61 aatcacgggg agttgttctt gaagcgtcgg tatagccgcc ttgaccagaa agtgcccaag
      121 tatatgaaat aagaccattc accacatagt aatctgcgct ggctgatgtg tagtcaacct
      181 cttcgccgcc actcagcact tgcgaagtat tgttaaccgt aacctgagcg tccccgctca
      241 ttaatgtttg gaacgttacc gttgcgatta ctccgttgga tgtatggccg ctggtacgcc
      301 ccctctgaat aactatgctc ccgccactgc atacatctgg ctgaggcgac agggtaaagt
      361 caggggagtt ggcaacgctc acacaggcca aacggctgct aggatacgta agctcggccg
      421 aaacagtatc aacggcttcc ccgactgtcg caagattgat ggtcatggtg aaggttgcgc
      481 ctttgctaac gttgtgcgcc gcaggaccca gcgataattg caccgatcct gccgcccgta
      541 tggagcgcac cggcagagca gcaaccacta tactggcaac cattaacaca attaaggcta
      601 gagccgaaca tttcatacag gttctaattt gttagtttta ttttcccttt tctagggtag
      661 tacggaccca ttatagctta ttggtaagcg taagcgcaat aatggtaaaa taggggagat
      721 gattgaagca aaaaaatccc ggccttactg gcacgtagat gcaaaatgga tatgcggact
      781 gcttctggta gtgatgctcg gtgccggcct gtttgccgtc gggctttacc ggcttaccga
      841 gcgatcaagc gcccaggcgc ttaccggtgc catcgccaca acttttttta tcaacccaaa
      901 tgctgacgtt agcgccaacg aagtgcgcca taagctacag ccgctttacg accaaggcct
      961 cggagccatt gccgagaccg cttcgcccga ccctaagaag caggctgaga ttaccaagca
     1021 attacgccca ctcgccctcc tcaccaacga cgcccacagc ttggtgtaca aaatcatctt
     1081 aggaatatca attgccaccc taatcctgac ggggggcctt atctttttta gccgtcgatt
     1141 cggcaagctt gtttcggttg gcgttgtttt gctggtacta gcattgccag gcacgctgct
     1201 ctttttgaca ttccaaagcg gcggtgcaac caatgggatg catataggat caattggacc
     1261 gccaatcctt acacttattg ccagcgcttt tttacctggc tatgccatag cccttgcgat
     1321 ggcggccggt ttattagtac tgagcggtgt cctcaaactc atccggcgct aaggcgctca
     1381 catcaaccta ttccacgtaa tcgccgaccc accaatcggc aacttcgcac aaaaagctgg
     1441 ttgcttcatt atgtttttcc gaattatgaa aattctgaaa atcctcctcg cttgcaaaaa
     1501 gagattccag aataataata tgtcccttac gcccatcaac cgactcgtga acctcccatc
     1561 gctcaagacc ttggcaccct tgccctgctt gatttaacag tgcgagagca tgctggtttt
     1621 gttcgccgga gatgccatct ttcaggcgaa atagaataat atgatgatat ttcgacataa
     1681 ccttgcttgg tataatgata acaaatgccc aacgccttaa agaaaaatcg tcatcgacta
     1741 tatctaagta taggtgccct agttatctta atgggagtta tcgcttttga ggttctgcca
     1801 caaatccagc aggcggccca tgaaaggaag gcaaaagcta atgcccttca ggcatgtttg
     1861 caagaccagg ctagcattcc caaaggaatg agaacatcag gaacctacat cgattgtcga
     1921 gctttatata aataggtgtg cagaaccctt atgtttaggt ggtagaatga ggagtatgca
     1981 aacaacacta ctcatgaggt tcttttcatt tttcatcttg gctagtgcat ggatggtcgc
     2041 catgccagga gaggcccatg ccgttccccc tattgtattc cccgtcatag gtccggccag
     2101 ttactcaaac gactttaacg caccccgcac caatggcatt catggggcca ccgacatctt
     2161 tgcccctaaa ggaaggctgc tcgtttcgcc agtaaacggt accattacgt cggtcatcgt
     2221 cccgcaagcc tcatggggtt acagtgttac ggttcgtgac accgaaggct attcattccg
     2281 cttcattcat atgaataatg acactccagg aaccgacgac gggcaaggcg gccccatgaa
     2341 tgcatatgca gcagacatga agaccggcaa tccggtagta gccggccagc tactgggcta
     2401 tttgggcgac tccggcaatg ccgaaactac cgctcctcat ttacattttg aaatttttgc
     2461 ccctgacggg tcactgatta acccctatga ttctctcaac cgggcgagta gaattcctgg
     2521 cccggtacat tatccggcgc ttcccaatga agtactgcct tacccgggcc taaccggacc
     2581 tatgaaaatt gccatgggta attttgaggg cagtagtacg acgtctgaaa tgatcaccgg
     2641 tgctggcagc ggtgcacctc ctcacgtaat tgcagcccga accgacggca ccgttctcgc
     2701 cagtttttat gcgtactcgc aagggttcag gggcgggatt gacgttgccg ctggtgacat
     2761 tgatggtgat ggcatagatg agattatcac cggaaccggt ccgggcgccg gtccgcatgt
     2821 catggtctat aagttaaatg gtacgcccgt agccagcttt tatgctttca acccaagttt
     2881 taaaggaggc atatcggtaa gtgccggtga tttggatggt gacggcaacg acgaaattgt
     2941 ggtcggtgcc ggaccgggag ccggtccgca tgtcatagcc tataaggccg acggctctac
     3001 cgtagtagcc agtttttatg cctacgccca aggctttaaa gggggtgtag atgtagccgc
     3061 cggagatgta gagggcacca ctgatgccga aattattacg ggtgccggac cgggtgctcc
     3121 tccgcatgtt atggtttacc agccagatgg cacctcgctc ggcagctttt acgtctatga
     3181 tcaaggaaac cggtctggag tccgggtaag cgctggtaac attagaacga gttctacaaa
     3241 agacgagatc ctcaccgctc ccgaagcact tccgggcggg ccgctgatta aagtagtaac
     3301 atatgacggc accaccatct cgcaatttaa ttttatggaa tcgtggtggt tagggtatca
     3361 cgatattgga gccggatatg ataccagccg agctgctacc ggtggcaatc ggcgcgcatc
     3421 agtgagagtt gggcccagtt aactagccgg taagcatgtt aaccataatg tgggggaatt
     3481 atgaacgaag atattcaaac ctacaataat ggccaggagg aaacaagtaa ggcactgtgc
     3541 gaattgctca ttcatgaaat acgactagct cttccgcatg ctgaaagtaa aatttggcat
     3601 ggtgcgcccg tctggttctt aagcggtaat ccgattgtcg gctacgatgt gctgaaagat
     3661 tgtgtcagat tgttattctg gagcggccag tcatttaatg agccggggtt gaaaaaagaa
     3721 ggcagcttca aggccgcaga agcacgctat actgacacaa gccaagtcag taaagttgat
     3781 ctagcgcgct ggttaaagaa atcaatagaa attcaatggg attacaaaaa tatagtgaaa
     3841 cggcgcgggg tgctagaaag gctgaggtag tataatcaat tcatgatcac aaaagaaatc
     3901 agcggcggtg tggcacatga gatgccagcg gacttgagaa aggcccttac ttccacctcg
     3961 acggctttag ctgcctggga agacatcaca ccgctcgcac gcaatgaatg gatgtgctgg
     4021 gtggaagaag ccaagaaaac ccaaacgagg gctcatcgca tcgagagaac gcgcacagaa
     4081 cttatggaag gaaagcgccg cccctgctgt tgggccggct gcaatcatcg ttgataagac
     4141 agtgttggat tttgccttag atgatacagg gcggttagca attgctctag gcgtttgatt
     4201 acagtcagct caaagcctag cttatctatt cgccgagccc ggcttctcct ctgttgccta
     4261 gcgtggcata caatgacata cttagtgata ggcatcaacc ataagaagta tgttcaataa
     4321 acatctcatt atcatgttct tcggttctgg aaaaagctga tgcattcaat caagcgcttt
     4381 gccaaggact acagcttgcc gttggcagca actgtgctgt tcgggttgct cataactggt
     4441 tcggtggttt tgcgcatgtc ccaacgggca tcgctcgccg actggctgga tgctgccacg
     4501 aatgatggca aatactctag cacgcttctt agtcaggaca aaccagagga agtcacgcgc
     4561 ggtaatgata ctccacagca gttgacacag actccggagg gcagtccgac ttcatttgcg
     4621 atttctccta acaatcagcc agttgcaaat ccatctaccc cgccaggagg tggaacacct
     4681 ttgtcgccac tatttagtgt ttctatagct tatttccaaa aaggggagac gactttagag
     4741 tgctcgactc caaaacctaa acccaagacc tgttcaaagc aatatgtatt taatggtggc
     4801 gtccggacac agaacggtcc aggaaacgtt acatatagtt ggcgcagcag catccaaagc
     4861 ctgacagcaa gtggcagctt cactgccagc agaggcgagg cgctcgctcc tttgcagaaa
     4921 atagtcacca tgccttgcga cacggttagc gactttagct tgcagctggt agtactatca
     4981 ccttcatttg tccaatccac cgtgtttaac acgaaccata actgcaatga gatctaattg
     5041 atggatgcga tgcataaaga gcaccctagc aacttgcagc gccactttat tatgtgggtt
     5101 ttgctgcccc cttttggagt tttcctggca ttaagtattc ttggtctttg gcagcttgaa
     5161 gtgatgctgc accgccaggc tattggtgac ctacaacgtg cctcaaccgc cactgccgca
     5221 aatgttaatc agcaattttc cctgcacgaa accgttctca agcaaatggg caccgaactg
     5281 tttgttatta aaaatgaata ccgcacaaat agaaagaatc ttgaagacaa ttataatgcc
     5341 tgtcgggcat atcttctcaa gtatgctacc cccaaattag ctccaaatgg gacatgccaa
     5401 cccttcttgg gcgggctagg tactaccggc aacttggtag cattggaaga tgagcatgtt
     5461 aggttagttg aaaaacttat tcagaaccaa aaccagcgca ttaatgagcg gctagcggcc
     5521 ttcaaagagt tttttccgga gaccctagcc ttactaatca ctgatggtag caaacaaacc
     5581 gtaagctctg ccctctccgg tgccttccag ggttctacgc tacactttca agctgatgcc
     5641 gaggcggcgc tgtccaagcc tatcagaggt aagatagcag aggttgaagg cttcaaaatg
     5701 gccatatttg gctttcccat aggggatggc agcgtcattg cggcgtatga cttacagaac
     5761 aaaaactttg tgcgtcgaac ttggtcgaat gctcctgtcg acaagacccg agagctggtt
     5821 gttctgctcg attcttcggg caagccggcg tatccccagc ttagggacgg caaacaattt
     5881 gctggcaaaa ttaatgacct gcgccataag ccgttcgttc atatcaagct agacaatgtg
     5941 gatcataccg ttgtaggcac gccaattgca ggttcggact ggatggcggt agtagcatcg
     6001 ccaacggctg caatacatgc tgcctcgcgc gatgctcagc ttgctgggct gatgcttatt
     6061 gggcttttta tgatagggtt tttgtgggtt ggcacttttt tcattcagcg cacacttcgt
     6121 aatatcatca acctagcact tggggcgcag atttttggct caggaaagtt agaccacacc
     6181 ataaccttga aacatggtga cggcgagttt ctccaattag ctgacaccat gaacggcatg
     6241 gcgcggcgca tagcggccgc ggagcaagca attgatgaaa agaacaagga gtttatatca
     6301 attgctaccc atgagctgcg atcgccactt acggccatga ttattaatct ctctgcattt
     6361 caggaaatgc atgtaaagaa tttagatgat gaagcccgtc attatgcaga tgaagcctat
     6421 gcaagcacaa tacgcttgcg cgacttagtc aacgacatgc tcagcatggc gcgcttggaa
     6481 agcggcaacc ccgagtttac catcgacgaa gttgacatag acggtttggt taagaacatt
     6541 gtcgatatga tgtctggtgt ggccgccaaa tccaaagtcg agctttccta tcaggataaa
     6601 tcggttagca aagttatggc cgacgagcaa tatctacggg tcatcctcaa caactttgtg
     6661 tctaacgcta tcaaatataa ccggcctggc ggaaaggttg tgatatcgca tgagttaaag
     6721 ggcgataaac tggttactat agtcgcagac agcggactgg gaattccgga cccgcaaaag
     6781 gcgcatattt ttgagaagtt tttccgaata cgcgatactg accgtaaaga cattcctggc
     6841 accggcctcg gcatgtacat tacgaaacag tacatcgaac agatgcacgg tgaggtgtgg
     6901 ttcgaatcta cccacggcaa aggcactacg ttttacttta cgttacctgt cgccaaaaag
     6961 cccccgagct aattctgtac attgcacgcg ccgtgtcgtc tagtaccagg ttgtcctata
     7021 gttccttgat tccccctacc ccgcatgtta cgaactgggt atttgcacca agtcctcgaa
     7081 atgggccgac tgcttcagct taatgagaat ttaccgatta gctagcttct gttcgaacat
     7141 aggcagcttt tatccagcca gcaacttcat catctagctc gtccactgag cgtagctcaa
     7201 tctcattgtg gtatcgattc gccgacactc tttctgtttt tttaatgcga ctactcttaa
     7261 tcggctcaga aagaacaata tttaagagaa gcccctcttt tctagggtgc acacccagaa
     7321 aggctttttt attgataatg tgcaatgaag tcttcttact ctcagcttgg tactcgggga
     7381 acttattaag taagtccagc aacttggcat aaatgcttgc tgaagcaggg ttgctttgaa
     7441 ttttatttaa tctatcttcc atagctaaaa gaataccaaa tggacaaatt gatctttatc
     7501 ctaacggcct atttccggtc atcctcctgc tccttatccg tttcagcatc tagcttttca
     7561 cggaatgata gttcgcgctt aataggccgt tccttacggc tttgatcact cagaggctca
     7621 aaaatcatat tgtgctcgtg ttcgtcatat ttgattctta cttcggagcc ttcgccaata
     7681 tggccgctta atatctcttt agcaagtcga ttttcgatct cactcttaat ctgtcttcga
     7741 agttctcgag caccatactc aggcacatac cctacatctg ctaaatggtt tattgtcgac
     7801 aggtcgaaat caagttcaat tgcttggcct cgcgccatgc gcttgacccg ttcaagctgt
     7861 aacccaacaa tctccttaat ctgatctttg gtgagggaac ggaatacgat aatctcgtca
     7921 atccgattga taaactccgg ccggaagtgc aatcgaacta cttccatgag ctcttgtttt
     7981 agagccgcat agctcttctt ttctacggta tcgttttgtc gggcttggct ggattgtccc
     8041 gccgttaagc tgtcttggat aatctgggcc cctagattcg aggtggagat aataatggtg
     8101 ttactgaaat caaccactcg cccttttccg tcggtcagtc ggccatcgtc aaatacttga
     8161 agcaggatat tgtgaacatc tgggtgcgct ttttcaattt catccagtaa taaaacgctg
     8221 taggggcgac gacgcactcg ctccgtcaac tggccaccct catcatatcc gacatagccc
     8281 ggaggcgctc caatcagccg cgaaacggta tggcgttcca tatactcact catatcgatc
     8341 cggatgatcg cgtcctcgtc tccaaaaacc tgccaagcta gtgctttggc caactcggtt
     8401 ttgccgactc ctgtcggccc caggaacatc aaggtagcaa tgggtcgact gccttcgcta
     8461 agaccagccc tggacatgcg gatggcatcc gacacggccg taattgcctc ctcctgacca
     8521 ataacccgct cgtgcagatg ctcttccatt ttcagtagct tttcccgttc ttcttgagtc
     8581 aactcgctta ccggaatacc ggtaatgcgt gagaccacct gagctacatg ggctgaaagc
     8641 acctcctttg aggttacgcc tttttgtgct cgccaggtat cgctcagctt ttccttcttc
     8701 tttcccaaat cacccagctg tttttcaatc ttcttggctt catcgaactg gcggtggtta
     8761 ctggcatact cgcgttcacg ttcttgccaa gcaatctgat tatccagctc ggtcatttcc
     8821 tgcgtgtaag tatcggctcc aatgcgtaca cgtgaggctg cttggtcaat taggtctatt
     8881 gccttatcgg gcagaaacct gttagctatg taacggtcgg agagtccggc agccgcgact
     8941 atcgcctcat cagtgatttt caccttatga tgggcctcgt agcgatcccg tagacctcgc
     9001 aatatctcga tagtctgttc aaccgtgggt tc
//
LOCUS       DASVGB010000034         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1114939, whole genome shotgun sequence.
ACCESSION   DASVGB010000034 DASVGB010000000
VERSION     DASVGB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114939"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..665)
                     /gene="pilO"
                     /locus_tag="VF272_01015"
     CDS             complement(120..665)
                     /gene="pilO"
                     /locus_tag="VF272_01015"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7259494.1"
                     /translation="MNPKRLFYVLCGLLLAAIGGGGFAYYASSQHFNAGVAKLSQRLS
                     ESQAADTNLTKLADLQKQYKQLGPYLPLINESLPAEKNQSKVALQLQNIATASGMRID
                     NIDFPASSQPGPISQTVPIGDVLALQVTFQLVGNYDQLQDFLRRQERLDRYSSMTSLN
                     IGPAEAGRLSFSIILNVFVKP"
     gene            complement(662..1204)
                     /locus_tag="VF272_01020"
     CDS             complement(662..1204)
                     /locus_tag="VF272_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259495.1"
                     /translation="MINLLPPEIKTQVAYSRYNRSLLRYLWLTLLLALILAGSLLAAT
                     YFLNQDIKRIDTDLAAKQHQIKAYESLQAKAKATGARLASIGTIQKNQAKFSILLADL
                     AQYMPQGTAISSIILTGDDKQPVRLTVNAVDYKTALSFRDSIARSPRISAADIENIKR
                     QPTNDYAVVVVFAFKPGVAK"
     gene            complement(1201..2253)
                     /gene="pilM"
                     /locus_tag="VF272_01025"
     CDS             complement(1201..2253)
                     /gene="pilM"
                     /locus_tag="VF272_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX7259496.1"
                     /translation="MRVPLIYKDAPQYGFDIGTHNVKIVQLRKAGSKTVVQGYGSAYF
                     PPESVVEGIVTDPLAMAKAIKPLLHKLSYGKLTSHRVVTGLPAGKLFTRTLQLPPMSA
                     SDLHQAIAYEVEQYVPVPVADLYIDHEVVNTTPGKDGRMDVVMMAAPRAIVDSYLKLF
                     DELGLEVGAIEAGMSAVVRALLHSGDAGGSTLVVDIGSISSDLTIYDTFIPLTGSVPV
                     GGQHYTDALVSQLGIKVEEANEIKEKFGLTPSGMHDKVYPALEPQLQTLVKEIKRVIK
                     FYESRHEEPKKVASLVLSGGTAQMPGLDEYLRQQVGLPLTVGDPWKNLDIKKANHPQE
                     TPMYVTAIGLALRGPV"
     gene            complement(2330..>3615)
                     /locus_tag="VF272_01030"
     CDS             complement(2330..>3615)
                     /locus_tag="VF272_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259497.1"
                     /translation="MSAGALNAAEAGAEIFMNNINNDSTYQGTNNAPGTATDSCTGYT
                     SAPVTFVENAIQGKVTYETCVKDGTITGEKMVYATAKVYLPTTAASPRVTRKIRLIIN
                     QALPASYTIMSGPGGLKLGNNVRITAGPIYVGGKLEMDNNAVIGSIATPVTTYVADKA
                     CPIPANATFPDFCPSGNSITTDTNSHVYGDTHVLNNVDAPSRFTHNGVVDNIVPPLNL
                     PPADHATLTSGLSNTTGMSSLNCPNNGTLHLSGHYTGTSTSGLTIGNNCTILLDGNVW
                     LDGNLALGNNSTLRPGDSVATPINLIIDGSTGLTTGNNNFITANASGVGFNVYTFWSA
                     DTTCRPNCSNVIGAALADSQGITTINLSNNVSGSANILFYSRWTKLMLNNNITVGQLL
                     GQAIELNNNGNIVFSSNTSTTPGGWDVRYYERLYR"
BASE COUNT          864 a          841 c          976 g          934 t
ORIGIN      
        1 ttgatagccg gtatagccaa agaggcagcc gataataacc agcacgatga cgcggcgctg
       61 tttattgagc gccggcagaa gcttggaggg attcagatct tttaacgata tattttgatt
      121 catggtttca caaaaacatt aagaattatg ctaaaactta ggcggccagc ttcggcgggt
      181 ccaatattga gggaagtcat actgctgtat cggtctaggc gctcttggcg gcgcaggaaa
      241 tcttgaagtt gatcatagtt tcccaccaat tggaacgtaa cttggagcgc caggacatca
      301 ccgatcggta cggtttggct gatgggccct ggttgactcg aagcggggaa gtcaatgttg
      361 tcaatcctca tgccgctggc cgtagcaatg ttttgaagct gcaaagctac ctttgattgg
      421 tttttttcgg ctggcagcga ttcattaatc aatggcaaat agggaccgag ctgcttgtac
      481 tgtttttgca agtcggctag ttttgtaaga tttgtgtcgg ccgcctggct ttccgagagg
      541 cgctgagaca gcttcgctac cccggcgtta aaatgctggc tggaggcgta gtaagcaaag
      601 ccgcctccgc cgattgcggc taggagtaag ccacagagaa catagaacaa gcgtttcggg
      661 ttcatttcgc aacccccggt ttaaaggcaa aaaccaccac gactgcgtaa tcatttgtgg
      721 gttgacgctt gatgttttca atatcggcgg cgctaattct tggagaacga gcaatgctat
      781 ccctaaaact tagtgcggtt ttgtagtcaa cggcatttac cgtgagtcgg accggctgtt
      841 tgtcgtcacc ggttaagatg atgcttgaaa tagcagttcc ctgcggcatg tactgagcca
      901 gatcggccaa caagattgaa aatttggcct gatttttctg aatggtgccg attgaggcca
      961 gacgcgcccc cgttgccttg gctttagcct gcaagctctc ataggcttta atttggtgct
     1021 gtttggcagc caagtcggta tcaatccgct taatatcctg atttagaaaa taggtagccg
     1081 cgagcaggct gcctgccaaa attagggcca gcagcagcgt tagccacaag tagcgcagca
     1141 gcgagcggtt atagcgggaa taggcaactt gggtcttgat ttcgggtgga agcagattga
     1201 tcataccggt cctcgcaagg ccaacccgat agccgttacg tacattgggg tttcctgagg
     1261 atgattggcc ttcttgatat caaggttttt ccagggatca cctaccgtta agggcaaacc
     1321 aacttgttgg cgcaagtatt catcaagtcc gggcatttgg gcggtaccgc cggaaagtac
     1381 gagtgatgcc acttttttcg gctcttcgtg acggctttcg taaaatttga taacccgctt
     1441 aatttctttt accagcgttt gtaattgcgg ctcgagagca ggatacacct tatcatgcat
     1501 gccactgggg gtaagcccaa acttttcttt aatttcattg gcttcttcaa ctttaatgcc
     1561 tagctgtgat actagggcgt cggtataatg ctggccgcct accggaacac tgccggttag
     1621 ggggatgaag gtatcgtaga tggtaaggtc ggatgaaatt gaaccgatat caacaaccag
     1681 cgtactgcct ccggcgtcac ccgaatgcaa aagcgctcgc accacggccg acatgccagc
     1741 ttcaatggcg ccaacctcaa gaccaagttc atcaaataat ttcaaataag aatcaacaat
     1801 cgcgcgggga gcggccatca tgaccacatc catgcgtcca tctttgccgg gggtagtatt
     1861 cacaacttca tgatcgatat aaaggtcggc aacggggacg gggacatact gctccacctc
     1921 gtaggcaata gcttggtgca gatcgctggc gctcattggc ggcaactgaa gggttcgtgt
     1981 aaaaagcttc cctgccggca ggccggttac cacgcggtgg gaggtcagct ttccataact
     2041 gagtttatgt agtaacggct taatggcttt tgccatagca agcgggtcgg taacaatgcc
     2101 ttcaaccacc gactccgggg ggaagtaagc tgatccatat ccctggacta cggttttgct
     2161 gccagcctta cggagctgca caatcttaac gttgtgggtt ccgatgtcga agccgtattg
     2221 cggggcatct ttataaatta atggaacacg cacactcacc tcattcaagc attaccattt
     2281 tagtatagca aagctctagg taagaaacga gtgctgccaa atgcagacgt catcggtaaa
     2341 gcctttcgta gtagcgaaca tcccacccgc caggagtcgt ggatgtgtta ctggagaaga
     2401 caatattgcc gttattgttc agctcaatgg cttgcccaag cagctgcccg accgtaatgt
     2461 tattattcag cattagctta gtccagcgag agtaaaagag gatgtttgct gaaccgctga
     2521 cattgttgct cagattgatc gtggtaattc cttgggagtc agccaaggcg gcgccgataa
     2581 cattgctgca gttcggccgg caggtggtat cagccgacca aaaggtatag acgttgaagc
     2641 cgactccgct ggcattagcg gtaataaagt tgttattgcc ggtcgtaagc ccggttgaac
     2701 cgtcaataat aagatttata ggcgtagcca cgctatcgcc aggcctcagg gtcgaattat
     2761 tcccgagcgc aaggttgcca tcaagccaga cgttgccatc gagtaatatt gtgcaattat
     2821 tgccaattgt tagaccgctc gtgctagtac cggtatagtg gccagaaagg tggagcgtgc
     2881 cattgttggg acagtttagt gacgacattc ccgttgtgtt tgataatccg ctcgtaagtg
     2941 tggcatgatc ggcaggaggc aggttaagcg gcggtactat attgtcgaca acgccgttgt
     3001 gggtgaatcg tgaaggtgca tcaacattat taagaacatg cgtgtcacca tatacatgag
     3061 aattagtatc ggtcgtgatg ctgtttcctg aggggcaaaa atccggaaaa gtagcgttag
     3121 ccggaatggg gcaggcttta tcggcgacat aggtggtaac cggcgtggcg atactgccga
     3181 tgaccgcatt attatccatc tcaagcttgc ctccaacata gattggcccg gcggtaatgc
     3241 ggacattatt gcccaatttt agccctcccg gtccgctcat tatggtgtag cttgcaggaa
     3301 gtgcttggtt aatgatcagg cgtattttgc gggttacgcg cggactggca gcggtcgtag
     3361 ggaggtaaac tttcgccgtt gcgtacacca tcttttcgcc ggtaatggtg ccgtctttaa
     3421 cgcatgtttc gtacgtaact ttcccctgga tggcattctc gacaaacgtt actggagcag
     3481 aagtgtagcc ggtgcaggag tcggtagcgg tacccggggc attattggtg ccctggtagg
     3541 ttgaatcgtt gttgatatta ttcataaaaa tttcagctcc ggcttccgca gcgttcaagg
     3601 cccccgcgct catta
//
LOCUS       DASVGB010000035         6936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_913860, whole genome shotgun sequence.
ACCESSION   DASVGB010000035 DASVGB010000000
VERSION     DASVGB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913860"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VF272_01035"
     CDS             complement(<1..398)
                     /locus_tag="VF272_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7259498.1"
                     /translation="MTLFVISFLAGALTVLAPCILPLLPVIVGGSLANADERKWFRPL
                     VITSSLAVSIVLFTLLLKAATALLGVPVWVWSAISGVIVVLFGISLLFPNLWVRLMVA
                     TKFNLRSDALLTKSYAKKGLMQDVLIGASLG"
     gene            complement(395..865)
                     /locus_tag="VF272_01040"
     CDS             complement(395..865)
                     /locus_tag="VF272_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7259499.1"
                     /translation="MNKLTIIAASAVICILGAIAYVALQPSRPGPFPTQTTKKINPAP
                     QPAKLGSSMQPGSYEAYASEKITATSNTKILFFHAPWCPQCRKLENDIKMQGVPEGVS
                     IFKVDYDSNQALRKRYGVTIQTTMVKIDDQGNLVEKYVAYDEPTFANVGANLLP"
     gene            complement(963..1223)
                     /locus_tag="VF272_01045"
     CDS             complement(963..1223)
                     /locus_tag="VF272_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259500.1"
                     /translation="MTDEQKRLAILGVLLKDGGWMRSFEIDVALQWRPSTAYPVLREM
                     REDLEARWEPVGKGAGLKKRQYRVKAGKVSALKKELKLARGE"
     gene            complement(1815..3473)
                     /locus_tag="VF272_01050"
     CDS             complement(1815..3473)
                     /locus_tag="VF272_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX7259501.1"
                     /translation="MIRPSLQLSKPTILLYVLLFGFAGTLIVFVSQASGSSTITGTVF
                     QDINKNGLQDPGEAPLANKSVYLIDEAGVTRLGFGVSDGAGKFAFTGLNDGKYQVVYG
                     SNDWWEIRADWVPTTTGSVAPKRVVNLTGQATVNFGWRQIVRSFDLNNPITSLTAPNG
                     IKINSYDDVVSATELYAELAKGSLSGVEASVTRIDFDYPVAPDPVTGVRNYNNNQPNI
                     TFSGVNGSPGYFSGYTATIKLGYISWLDSGDSLMFHEYGHAWSLRYAYIEAQNTDFIE
                     YLKVRGIENDSRLDSTHGWSRREMIAEDYRQLFGSPNAQAHPQENRAVPLAKDVPGLK
                     EYLAGAFMQSAAPLPPPPPSADISAPSAPIGLVGRTVSGSEGIEIDLSWTASVDNVGV
                     ADYLVFRDGLQIGSVNSPSTSFIDTTNIAPGASYRYYVRAVDVANNISTASQTITVTV
                     PAPDTLSPSTPSGLISPSQTNNSIALKWNASTDNVGVSAYRIYRIGGSRKLALNTLVG
                     TITGSTFTATGLSRGTSYSFFVVAVDAAGNVSSPSSTLVVKTKR"
     gene            complement(3984..4331)
                     /locus_tag="VF272_01055"
     CDS             complement(3984..4331)
                     /locus_tag="VF272_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259502.1"
                     /translation="MAIDDVVLTDADRANPDHMKGERPYCREPRTVKIRVEVQPGHGI
                     KTTLGPFQLTDRDDKLGFSRVAQAVVAEREAVCATNDEVRALIAGAVRACAPACNADR
                     VADIVLADALYSD"
     gene            complement(4471..4547)
                     /locus_tag="VF272_01060"
     tRNA            complement(4471..4547)
                     /locus_tag="VF272_01060"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4511..4513),aa:Asp,seq:gtc)
     gene            complement(4602..5447)
                     /locus_tag="VF272_01065"
     CDS             complement(4602..5447)
                     /locus_tag="VF272_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259503.1"
                     /translation="MFKILRNMAVVAVLMVGAYLGFHYQDQILDWYYLRGYTPPARVA
                     ELADKATMTENGRRIFYRAKPQMLKDRPSLVKSCRVEGEKTIELGCYLSTNQIFLLNI
                     QQPDLKDEEIVTAAHEALHAVYDRMSNKERREINTRLRAAAATITDSSLAERLSAYRQ
                     LEPGEEDNELHSIIGTEYPNLSPELERHYLKYFTNRAQIVSYSQSFNQRFDGLHKQIV
                     QLDAEIQAIKLRMRQYLEAGRISTHNALVPHVNELITEYNQKVEQYNQYASDLLGQQA
                     PTGSE"
     gene            complement(5483..6559)
                     /locus_tag="VF272_01070"
     CDS             complement(5483..6559)
                     /locus_tag="VF272_01070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7259504.1"
                     /translation="MEEEKKPAAKKSASKSTKPAKSASKAKSTEASAAPIVTTAAATP
                     SKSSGSAGSPGNHRMWWMIGGTVGALAVLFLLVFGILIYKYQNDSRIVQLVSQVVPYP
                     AERVNGSFVSYSNYLFEVNSIKHYYLSQTGADNKPAVDFTTADGKKKLKELQRQELKR
                     LQQETVIKQLASKNKVKVTDKEIDQQVDQITKSAGGEAKVKDVLKKFYGWDINDLQKK
                     IHMQLLQQKVAAKIQSDPKLAAQAKTKADDVLKKVNGGGDFAALAKQYSQDSSAANGG
                     DLGFFSKGQMVKPFEDAAFGQEPGQVSKLVKSQFGYHIIKTLEFNADKSQVHAAHILI
                     KTVDFDQFLKDEVAKAKVNQFIHP"
     gene            complement(6593..>6936)
                     /locus_tag="VF272_01075"
     CDS             complement(6593..>6936)
                     /locus_tag="VF272_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7259505.1"
                     /translation="FKIMLITPVLVLLYWFYYWVGWYYSVYIVTDQRLIDIQQKGLFN
                     RKVNEVGFDKIQSINYHINGLQAALLKYGDITVLTYANDWKLPCVHHPEAVHQEMMAV
                     ARMVNSTPPQK"
BASE COUNT         1701 a         1778 c         1684 g         1773 t
ORIGIN      
        1 cctaaagagg cgcctatgag gacatcttgc atcaaacctt ttttggcata cgatttggtc
       61 agcagcgcat cggaacgcag gttgaacttg gtcgcaacca taagccggac ccacagattg
      121 ggaaataata agcttatacc aaaaagcacc acgattacac ctgatatcgc actccagacc
      181 cataccggca cgccaagcaa ggcggttgca gccttaagga gaagcgtaaa taggacgatt
      241 gatactgcca aactgctggt aataaccagt ggccgaaacc attttcgctc atcggcgttg
      301 gccagcgaac ctcccacaat taccgggagt aacggcaaaa tgcagggagc cagcaccgtt
      361 agtgcccccg cgaggaatga gataacgaaa agcgtcatgg cagcaggtta gcacccacat
      421 tagcaaatgt cggttcatcg taggcaacgt acttttccac caaattaccc tgatcgtcga
      481 tctttaccat ggttgtttga atggtcacgc cataccgctt tcgtaatgcc tggttgctgt
      541 cataatccac cttaaaaatg gaaacccctt cgggaacacc ctgcatttta atgtcgtttt
      601 ccagcttgcg gcattgtggg caccatggcg catggaagaa aaggatttta gtgtttgagg
      661 ttgcagtaat tttttccgat gcatacgctt cgtatgatcc aggctgcatc gaactgccga
      721 gcttggcagg ttgaggagcg ggattaattt ttttagtggt ttgggttgga aacgggccag
      781 gccgcgaggg ttgtaacgcc acgtacgcaa ttgcccctag gatacagata acggcagaag
      841 ccgctatgat agttaacttg ttcatagctg taataatagc agagccttac acgcgatgaa
      901 gtcccctgta cgaaagaggt ccacggtgga gaccgagagc ctcttccgta gaccttgttg
      961 cgttactcgc cgcgtgcgag cttgagttct ttcttcaggg ccgaaacctt gcccgccttg
     1021 acccggtatt ggcgcttctt gagccccgca cctttgccga ccggttccca gcgcgcttcg
     1081 agatcctcgc gcatctcgcg caacacgggg tacgcggtgc ttggcctcca ctggagggcc
     1141 acatcgattt cgaaggaacg catccatccc ccgtctttca acagcacacc gaggatggca
     1201 agccgcttct gctcatcggt catggcgaac ccctttccgc ccggctgtcg agcttgtcat
     1261 agatctcctc acgcacaatg gccacctcgc gaggagcttt gaatccgagg caggtgtacg
     1321 tcgctccgac cctgagcacc ttcatttgtg catcgcagcc cagagcaatc gactcctcct
     1381 tactcagggt gtatacctcg gcagagtcgt cgatgtgact ggttacccgg actcgaaccc
     1441 cttgcggaca caccgcctca accatcacca ctgtgaccat gggtccaatg atgacggact
     1501 gtccttcacg ccgcacaagg ttaagcatgc gaacctccaa ggttcggccg attgatacgc
     1561 tttacttcct gcgtatggac tcaattatta tgctactcga tcaaaaaaat gcaagcataa
     1621 ggatcataca ttgcaccccg gagttggtta taccaacaca ggcgcaaccg ccgtcaccag
     1681 gaaggcagcc actatcaaga ttattgcgaa cttttttagc attctatcct actaccactt
     1741 atgctttcat tgttgcatct tgtgggggca ggttcaagtt ttaaattgtt ttggtatttt
     1801 ccgtagtgct tttgctatcg cttcgttttg acaaccagcg tactactcgg cgaactaaca
     1861 ttgccagctg catcaaccgc tacaacgaag aaggaataag aggtgcctct tgaaagtccg
     1921 gtggcggtaa aggtactccc ggtaatcgtt ccgacgagcg tatttaatgc aagcttacga
     1981 gagccgccaa tgcggtaaat gcgataagcg ctaaccccaa cattgtccgt acttgcattc
     2041 catttgaggg cgatagagtt attggtttgg ctaggtgata taagaccgct aggggtagag
     2101 ggcgatagag tatcgggcgc cggaacggta acggtaatag tttggctggc tgtactaatg
     2161 ttgttggcaa catcaacggc cctaacataa taccggtagc tggcgccggg agctatgttc
     2221 gtagtatcaa tgaaactcgt ggatgggctg ttgacggaac caatttgaag gccatcacgg
     2281 aaaaccaggt agtcggcaac acccacatta tcgacactgg cagtccagga cagatctatc
     2341 tcgatacctt cgctccccga gacagttcta ccaaccaaac caatgggagc tgaaggagca
     2401 ctaatgtcgg ctgatggggg cggaggcgga agcggggcag cggattgcat aaatgcaccg
     2461 gcaagatatt ctttaagacc gggaacatct ttggcgagcg gtacagcgcg attctcttga
     2521 ggatgtgctt gtgcgtttgg cgaaccaaac aactgccggt aatcctcggc aatcatttcc
     2581 cggcggctcc agccgtgggt agaatccagc cgtgagtcgt tttcaatccc gcgcacttta
     2641 agatactcga taaaatcggt attttgggct tcaatatagg cataacgaag actccaagca
     2701 tggccatatt cgtggaacat caaactgtct ccgctgtcga gccatgaaat atagcccagt
     2761 tttatagttg cagtgtagcc gctaaaataa cctgggctac cattaacacc tgagaacgta
     2821 atgttaggtt gattgttatt gtagttgcga acaccggtaa cgggatcggg ggccacggga
     2881 tagtcaaaat caattcttgt aacactagcc tccactccag ataaactgcc tttcgcaagc
     2941 tcagcgtaaa gctcagtggc ggaaactacg tcatcgtagc tattaatctt aataccgttc
     3001 ggagcggtaa gggaggtaat cggattattc aagtcaaaag aacggacaat ctgccgccaa
     3061 ccgaaattaa cagtagcctg tcctgtgagg ttgaccaccc tttttggagc aaccgagcca
     3121 gtggtggtag gaacccaatc cgcacgaatt tcccaccaat cattggatcc atataccact
     3181 tgatatttgc catcattaag cccggtaaat gcaaactttc ccgcaccatc cgaaacaccg
     3241 aaacccagcc gtgtaactcc cgcttcatca atgaggtaaa cacttttatt tgccagaggc
     3301 gcctcccctg gatcctgcag accattttta ttgatatctt gaaacactgt accagtgatg
     3361 gtggaactac cggaggcttg tgagacaaag actattaatg tcccggcaaa gccaaaaagc
     3421 agtacgtata aaagtatggt aggcttcgat aattgcagcg atgggcgtat cattggacct
     3481 cgcttgaccc ctctaatgag ttgcataata acaaatgtgc cttacacagc ttagctaatt
     3541 taagcataag tgctattttt ggcttggtca actaaaactt ttacttagac gtggtgagag
     3601 ataagtccag atggcgggcc cgaccggatt gtggcttgcg ccacctccgc tcgcctatcg
     3661 ggtcgctcca acgaaccgct gtttcgaatg acgagcaagc tcgtctatga acaaaaaagc
     3721 caccatgagg tggttttctt gttcatggcg ggagcttatt aacgattttc gaacttttgc
     3781 ctagacgcga cgaagcccca ctagggcggt agtccacatg gcttgcgccg gtgtgggaac
     3841 ttgtcgcacc agtagggctg gctacagaac gatgatcgct acgaccgtga ccacagccac
     3901 gaaagcgaga aaaggccacc atagatccat atttcacctc ctttcggacg attcagttgg
     3961 tcccaggaac ttccttgagc gcactagtcc gagtagaggg cgtctgccaa gacgatatcg
     4021 gccacccgat cggcgttgca tgctggagcg catgctctga ccgccccagc gatcagggcg
     4081 cgaacctcgt cgtttgtggc acagaccgct tccctttcag cgaccactgc ttgggcaacg
     4141 cgagagaagc caagcttgtc gtctctgtcc gtaagctgga aggggccgag agtcgtcttg
     4201 atcccatgcc cgggctgtac ttcaaccctg atcttcaccg tccgaggctc acggcagtat
     4261 ggccgctcgc ccttcatgtg atccggatta gctcgatccg catcagtaag cactacgtcg
     4321 tcaatggcca tgtgcttcct ttcgatcgga ggacagtgat ttactatagc agaaaatagc
     4381 tccgatggat aacgcttacg aatttatcta ggtaagctgt tcggtcggcg gtattgaaag
     4441 aaattgcttt gttaagggtt aaataattgc tggcgggccc gaccggattc gaaccggcga
     4501 tctcctccgt gacagggagg catgttaggc ctctacacca cgggcccgcg accggataat
     4561 cgtagcattt ttcaggtctc ggtacaatga gccatgaacc gctactccga accggtgggt
     4621 gcctgctggc caagcagatc gctcgcatac tggttgtact gctctacctt ttgattgtac
     4681 tcggtaatca gctcattcac atgcggcacg agcgcgttat gagtactaat ccgaccggct
     4741 tcgagatatt gacgcattcg cagcttaatt gcttgaattt cagcgtccaa ctgaacaatt
     4801 tgcttatgca gtccatcgaa acgctgattg aaggactggc tatagctcac aatttgggca
     4861 cgattagtaa aatacttcaa gtagtggcgc tctaattccg gagaaagatt gggatattcg
     4921 gtcccgataa ttgagtgcag ctcattgtcc tcttcacccg gttcaagctg ccggtacgcc
     4981 gataagcgct ctgccaagct ggagtcggta attgttgcgg cagcagccct tagccgggta
     5041 ttgatctcgc ggcgctcctt gttgctcatt cggtcataca cggcatgcaa ggcttcatga
     5101 gccgcggtta cgatctcttc gtcctttaag tcaggttgct gaatattcag taggaagatt
     5161 tgattggtac tgagatagca gcccaactcg attgtcttct ccccttctac gcggcaactt
     5221 ttaaccaagc ttggacgatc tttgagcatc tgaggttttg cacgatagaa gatccgccgt
     5281 ccgttttcgg tcatagtagc cttatcggcc agttcggcta ccctggccgg cggagtatag
     5341 ccacgtaggt agtaccaatc gagaatttga tcttgataat ggaagccgag ataggcgcca
     5401 accatcaata cggcgaccac tgccatattg cgcaatatct taaacatgca agactatcct
     5461 acaagttaga aaactggggg ttttaggggt ggatgaactg gttgacctta gctttggcaa
     5521 cctcgtcctt gaggaattga tcaaaatcaa cggtcttaat taagatgtgt gcagcgtgaa
     5581 cctgagactt atcagcattg aactcaagcg ttttgatgat atggtacccg aactggcttt
     5641 taacgagctt ggaaacttga cctggctctt ggccgaaagc tgcatcttcg aacggcttaa
     5701 ccatctggcc tttactgaag aacccgagat ctccgccgtt ggcagccgaa ctgtcttgcg
     5761 agtactgctt agccagtgca gcgaagtcgc cgccgccgtt aaccttctta agcacatcat
     5821 ctgccttagt tttagcttgg gctgccaatt taggatcgct ttgaatcttg gcggcaactt
     5881 tttgctgcag caactgcata tgaattttct tctgcaggtc attaatgtcc cagccgtaga
     5941 acttcttcaa tacatctttg actttggctt caccgccagc cgatttggta atttgatcga
     6001 cttgctggtc aatctcttta tcggtgactt ttaccttgtt cttagaagcc aattgcttaa
     6061 taactgtctc ttgctgcagg cgctttaact cttggcgctg cagttcttta agctttttct
     6121 taccgtcggc agtagtgaaa tcgacggcag gcttgttgtc ggcaccggtt tggcttaagt
     6181 aatagtgctt gattgaatta acttcaaaca aatagttgct atagctaaca aaactgccgt
     6241 ttacgcgttc agccgggtaa gggactacct gggataccag ctgcacgatc cgactatcat
     6301 tctggtactt atagataagg atgccaaaca ccaacaggaa taggacggcc aatgcaccaa
     6361 cggtaccgcc gatcatccac cacatacggt gattacccgg agatcctgct gaccctgatg
     6421 atttcgaagg cgtggctgcc gcggttgtaa caatcggtgc tgcagatgct tcagtgctct
     6481 ttgccttaga tgctgacttt gccggtttcg tcgacttaga cgcactcttc tttgccgctg
     6541 gcttcttttc ttcttccacc aaaaaactcc ttatgcttca tttaatgcta ccttactttt
     6601 gcggaggtgt cgaatttacc atccgcgcta ccgccatcat ctcctgatgt accgcttcgg
     6661 gatgatgaac acacggtaat ttccaatcat tcgcataggt tagtaccgta atatcaccat
     6721 actttagcaa tgcggcttgc aacccgttga tatggtaatt gatcgactgg atcttatcga
     6781 aaccaacctc attgacctta cgattgaata agcccttttg ctgtatatca ataagccgct
     6841 ggtcggtcac aatgtaaacg ctgtaatacc accctaccca gtagtagaac cagtacaata
     6901 atactagcac aggtgttatt aacattatct taaatg
//
LOCUS       DASVGB010000036        11511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_372923, whole genome shotgun sequence.
ACCESSION   DASVGB010000036 DASVGB010000000
VERSION     DASVGB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372923"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..549)
                     /locus_tag="VF272_01080"
     CDS             complement(175..549)
                     /locus_tag="VF272_01080"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259506.1"
                     /translation="MQRLGLVLAGVWMALPLPVLAGDTTKTNLCTGAGGAISGTGCDV
                     PGKGLTGDQGFVLTITNTLMTVAGAIAVIIIITAGIRYITSTGDAARVKASKDTLMYA
                     VIGLIVVILAYAIVRFIATSIG"
     gene            complement(549..902)
                     /locus_tag="VF272_01085"
     CDS             complement(549..902)
                     /locus_tag="VF272_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259507.1"
                     /translation="MKEKLLQLGVGALVFAPSPIIAACTSEQGKGLDAVSPGACSANA
                     LTARLNVIIDTLFIVIGAVAVLIMIMGGIRYITSTGDAKRIQAAKDTILYAVLGLIVA
                     ILARAIVGFVIGQIA"
     gene            complement(909..1310)
                     /locus_tag="VF272_01090"
     CDS             complement(909..1310)
                     /locus_tag="VF272_01090"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259508.1"
                     /translation="MKRIMIVIAMIVGAGILFASPVMAADDPATIIGNACDNAAFLPG
                     CGPDDQQLDAPGGNNNQSLLQQIINVFLFAAGIVSFIFVIIGGVRYMTSTGDATRIQS
                     AKETVLYAIIGLIVTVLAVPISAFIINAAGG"
     gene            complement(1352..1723)
                     /locus_tag="VF272_01095"
     CDS             complement(1352..1723)
                     /locus_tag="VF272_01095"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259509.1"
                     /translation="MKLLAIIAFCTAAAFMVFAIASTSSAIAASCPQAGCIADNPSEL
                     DVPNLTPTEVAEGIIRILSFIAGVASVAFLIIGGLRYSSSSGNSQRVESAKQTIIYAV
                     IGLILSIFSVFIVGFVISRAT"
     gene            complement(1761..2072)
                     /locus_tag="VF272_01100"
     CDS             complement(1761..2072)
                     /locus_tag="VF272_01100"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259510.1"
                     /translation="MIIPSFAALIPKLYQDPSTGQDIDFTSIDQIGIVIANVIQILLV
                     LTGILAVIFIIYAGILYIVSQGEAAKTASAKTALLNAVIGLILSSAAYLIVGFVARNI
                     Q"
     gene            complement(2069..2728)
                     /locus_tag="VF272_01105"
     CDS             complement(2069..2728)
                     /locus_tag="VF272_01105"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX7259511.1"
                     /translation="MKRHIRMFIVLMIAFGMPVLFTPEPASAALTRAQQATDFCKTYP
                     ITATASKISRSQCRTVFISGYNAGGPARATLCKPASLKGSKLTSCNAAFNAGTSAGTA
                     ARASAAAGTTNRTCPGKAIVVSEALVPGGCIGSANQNPIFALIGFGVQFLTGLFGIIL
                     VLVLVIAGFQYVISGDDPEIAKEAKDRIKGAFTGLVLFVIMFALIQIILPSELKIFTS
                     S"
     gene            2819..5731
                     /locus_tag="VF272_01110"
     CDS             2819..5731
                     /locus_tag="VF272_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259512.1"
                     /translation="MKRSRPRILFALIGYVFAMAASFLIPVLSNPVQAGEPVQPLNIV
                     FFYNETDKEAIKSGLAAIGSPITGGPGANANIAKTAIYAKGSNFGTNPFLFRYSGNTS
                     SAVGGSIRPIYEATYFCVGGSANPRIATAGGAGDAKVILHIIPSLTAAFIDASPTSPR
                     SVTGAMSLVSAEQNGSPRTMAQLPSDCRINGLPRTVTIPNYNLLTAATKSTWGPNLKT
                     SGQADAATNAPANNQAGTASAGVDEDAASQCPRSAAGLGWIVCPFIGIVNDLFARSFQ
                     KFIVGLMHIDPLDTSSTSTNLYGVWKNIRDLANVLFLLIFMIIVLANTLSFNVQAYTI
                     KKMIPKLVAAAIMVQFSFIISAVLIDIFNIIGGGLGDLLSSLANIPGDQGGSFLENLF
                     ATVFSALILVLGGIIGVVAVGSAIGTTGAFAGVAIGFIIVLALTAFFAFMSLLFSLMF
                     RKLIIVMLILMSPLAFAALVLPSTEKIFKVWIGTFIQILMMYPIIVFLFASATVLQQA
                     LGSSANDVNPSVGIPELMAAVMPIVVCFMVPMTFKWGGKLMSAGSNGIDSLVRGKIGG
                     SAVSKFKDSEAYKVPRALRASNKLEKKQGQASMSGTLKGMNKFNRWKAGVQLRGDFLG
                     GNQMIAVERGKFGLPTGLPKRGDYGDMRRIQIRQRNEYLDKGLANAALDRQHLISLLD
                     PMTKGAKDGNFDEVMKQPLLYDQVLNGLGSYVKKEGKLPYADALVDLTAMGTKMGKTD
                     KDLREDQDWIMGIDHNATKAMPLAAETTHVTNLANRQDIDETTGDLKTVHVDNNPNLA
                     PRKVKVASTSDHEWHDLEASFGQDTFMGDNKEIAVDQFVHGLVEQRDANGNVTQHRVI
                     DKLLAKDQLLLHTLHTTATRYGAVKTEARQAIVDYGTRALTRLDALEVKAQNNNLNAE
                     EADELNFATQNKLRAAYTFAAHFADDAKVYQDKFTGNSGATKDPALEQALKDVGGA"
     gene            5734..6570
                     /locus_tag="VF272_01115"
     CDS             5734..6570
                     /locus_tag="VF272_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEX7259513.1"
                     /translation="MGTYKVPQNVEAEDKILGPLSMKQFIYALIGLGYGLLTFAILKS
                     VILLWILVGLPPALALLALGLYQRQDQSMETYLISIAQFIGRPKSRVWQKEPVAEVFH
                     LEPPPPKPRMETRDSRKVRSQLAQLANVVDTHGWATKQPQVQEADQQIQVVDLNERIG
                     DITLPVEPRQEIHMPEVTDADDMLNISGAAAQNLDNLIANSIQSLRADVLEKMAAGSA
                     LPPMPSTSGMTTPPSGDIVKLAAEGGDLTVAQLASQARRQIAAIGEGAPASVQNASPQ
                     PN"
     gene            6548..7294
                     /locus_tag="VF272_01120"
     CDS             6548..7294
                     /locus_tag="VF272_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259514.1"
                     /translation="MPVPNQTNPAPKKPSTQDTLLIAEIRDGVVVMRDGSIRSVLMGS
                     AVNFDLMSAQEREGVEYSYQSFLNSLHFPIQILIKSQRIDLDSYLSNLAQLRQSEDNP
                     LLGALMDDYILNIQGLIEQVNIMDKQFFIIVPYLPPLMSKMKKKNVVTGLAGAFKKQA
                     VTTLNENDFKAFKDELAQRVSLVSAGLGQIGVRNIPLNTQELIDLFYSSYNPDVAQNQ
                     KLVDAGQLQTAAVTKGEGDAPQHLASGGPF"
     gene            7294..9159
                     /locus_tag="VF272_01125"
     CDS             7294..9159
                     /locus_tag="VF272_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX7259515.1"
                     /translation="MGLLGGKKEVNDPIAIAESQRAQEQQEAEAAYRQGIVTLRDLIA
                     PPSFSIANDHIQIGTRYARTLYVYGYPREIFTGWLSPIINLDEVMDISLFIYPVESQV
                     VLNNLKRKVGQMEATYAINQEKGRVRDPGLEAALMDAEELRDKLQVGEERFFRFGLYA
                     TVYADSMEELKDIQRKVEGIFGQSLVYTKPASMQMEPAFNSTLPLGTDELQVARNMNT
                     GALSTSFPFTSAELSRNEGVLYGINRHNNGLVLFDRFSLENANMVVFAKSGAGKSFAI
                     KLEAIRSLMLGTEILIIDPEDEYHSMCEAVGGSYLRLSLASSSRLNPFDLPRVLDSED
                     ADNALRANIITLHGLLRLMMGGQASPLSPAEDADLDVAIINTYSKAGITNDPLTHGAT
                     PPTMNDLYKTLSEMSGNGPALAQRLRQYTSGTFAGIFSEQSNVDLNNKFVVFNIRDLE
                     EELRPVGMYIVLNYIWNKVKSDKRKRILVVDEAWQLMKFEDSANFMFSIAKRARKYFL
                     GLTTITQDVDDFLSSRMGRAVVSNSSLQLLLKQAPSSVDIIGETFKLTAEEKTRLTNF
                     PVGEGLFFAGLNHVVMKILASNAETQLITTNPQAIAQQEAAAKAYREAQQEIAGA"
     gene            9163..>11511
                     /locus_tag="VF272_01130"
     CDS             9163..>11511
                     /locus_tag="VF272_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259516.1"
                     /translation="MQDDSTVTDDKEAIGDESAAAEERRRAMAAAARRRDMRSQHKRK
                     TDDGASQRRRGTNDLNDKYGLGGDDSGKKKLSKAKTAYLAMRAAAGSRTAISSLILGG
                     GFIAVVIGLFFIYAQYKLVHTMEGMERHFFSRNNHAIHARQQILMREFIFNHLDGENL
                     PDSNANAPFAVQVFNTLYKGDFKKRLEAQGVKITRRKSLNGLTTLVTIEDTRNGTKRT
                     YDQGNGQDRRDLGLLGEQLNDRLSGDGTNRIRAVVVKAHLSRRLGYHWHPLDPIRGSY
                     AEVRAKLFARMLNPALRIPALQSIASEVLKKFFGDTDEGREIQENLSAAIDSPDRAAG
                     KVALSAVITETTAKFVKNANLVGLAILAADVGCRISDFLADRTARKLVQKKIELEQMG
                     VFRDLRSAADQQKDGKNTDANTVSAAGELLAYTDKDGKVHDISESNNYSRQNGRPVEY
                     HPSTNCEDNRELCLQKQASKAMRATTLGSIFEGAGDLLNSPFYKALPLPLPGTTSTVV
                     GCTALRPVLNVVDSLFGLIGRGVDTVLSNTPGVNTLWSSIKDLAGDFITMVSNKFIGA
                     LAGPSIDIATTRGPALLNVAGAGASLAAGSIAGSPAGTDETLDENVCYNKTPEEKKQD
                     TSLCGKKLTRAQLVIIDNAIVQENHRQFNESSLWTRMASLDTPYSFASRIAFATPSSP
                     KVLMQRAGTTLTAAINPSGWMRFFELLPNLFTPKLQAVGSVPGSSKIVDSEGKDQFGT
                     DIYGFEEADVQSSGFDTKPHLHNLDTASGCSAISDYVEKKGRD"
BASE COUNT         3047 a         2899 c         2975 g         2590 t
ORIGIN      
        1 tgagcaagtt agcaatttgc cggaagctta cggtaagaga atccggtgtt cccgccggac
       61 gggcattatt ggcgccttca agtatcgtcg caaacgatgc cggggcgtag gtcaggcata
      121 tgattgatgc aaagctcagc cggcggagta tagaagtaat catatcattt tcctttatcc
      181 aattgatgta gcaataaacc gaacaatggc atatgcgagt ataaccacga tcaaacctat
      241 gacagcatac atcagggtgt cttttgaggc cttcactcga gcggcatcac cggtagaggt
      301 aatatagcga atgccggccg taatgataat aataacggcg attgctcccg caacggtcat
      361 aagagtgttg gtgatggtca gcacaaagcc ctggtctcca gtgagacctt tcccgggtac
      421 atcacaccca gttcctgaaa tagctcctcc tgcaccggtg caaagattgg ttttagtggt
      481 atcgcctgcc aaaaccggca gaggaagcgc catccaaacg cctgccagca ctaagcccag
      541 gcgctgcatt acgcaatctg cccgattacg aagccgacaa tggcgcgagc caagattgcc
      601 acaatcaaac caagaacggc gtagagaatg gtgtctttgg cggcttggat tcttttggca
      661 tcaccggttg aggtaatgta gcgaatcccg cccatgatca taatgagaac ggcaaccgca
      721 ccaattacaa taaagagcgt gtcgataata acatttaacc tggcggtaag tgcgttggcg
      781 ctacaagctc cgggcgaaac cgcatcaaga cccttgcctt gctcgctggt acaggcggct
      841 atgatagggg agggcgcaaa gactagggca ccaacgccga gctgcagtag cttttctttc
      901 atctgtacct aacctcccgc cgcattgatt ataaatgccg aaatcggcac cgccaaaacg
      961 gttacgatta atccgataat agcatataac accgtttcct tggcgctctg aatacgcgtg
     1021 gcatcaccgg tagaggtcat ataacgaaca ccgccaataa taacaaaaat aaacgaaaca
     1081 atgccggcag caaacaggaa gacatttata atttgctgta ataaagactg gttgttgttg
     1141 ccgccaggag catctagttg ctggtcgtcc ggaccgcacc cgggaagaaa ggcagcatta
     1201 tcacaggcat tgccaataat ggttgccgga tcgtcggcag ccataaccgg ggacgcaaac
     1261 agtatgcccg cccccactat cattgcgatg actatcataa tccgcttcat tggttgcatt
     1321 gtatcgaacc gcccaacagg tgtcaaaatg cttatgtcgc tctggagatt acaaatccaa
     1381 cgataaacac ggaaaaaatt gatagaatca ggccgatgac agcatagata atggtttgct
     1441 tggcgctttc aactcgctgg ctattcccgc ttgagctgct gtagcgcagg ccgccgataa
     1501 tgaggaaggc aaccgatgcc acgccggcaa taaaggagag aatgcgaata attccttctg
     1561 cgacctccgt aggggttagg ttgggaacat caagctcgct aggattatcg gctatgcagc
     1621 cggcctgggg acacgaagcg gctatcgccg atgaagtcga cgctatagca aataccatga
     1681 aagccgctgc ggtgcaaaac gctataattg ctaacaactt catgctcgga ttgtatcggt
     1741 tagggtgggc atggtcaaag ctattggatg ttacgcgcta caaaaccgac gattaaatag
     1801 gccgccgaag acaagattaa gccaataacc gcattcagca aagcggtttt ggcgctggcg
     1861 gttttagccg cctcgccttg agagacaatg tagagtatcc cagcataaat aataaaaata
     1921 accgccaaaa ttccagtcaa taccaggagg atttgaatta cattcgcgat tacaatgccg
     1981 atctgatcaa tgctcgtgaa atcaatatct tggcccgtac tcgggtcttg gtatagctta
     2041 ggtatgaggg cggcaaatga tggaataatc atgatgaggt aaagatttta agctcagacg
     2101 gcaagataat ctgaatcaat gcgaacataa taacaaacag gaccaagccg gtgaatgcac
     2161 ccttaatgcg gtccttagct tccttggcaa tctccggatc atcccctgaa atcacatatt
     2221 gaaagcctgc aattaccaaa acaagcacta aaataattcc aaataagccc gttaaaaact
     2281 gaacgccaaa accaataagc gcaaaaatcg gattttgatt agcgctgcca atacacccgc
     2341 cgggcactaa tgcttcgctt accacgatag ccttgccagg acaggtgcga tttgttgtgc
     2401 cggccgcggc agacgcccgc gctgcggtac ccgctgatgt gccggcatta aaggccgcat
     2461 tgcaagaagt aagcttcgag cctttcaggc ttgccggctt acaaagcgta gcccgggctg
     2521 gcccaccagc gttgtagccg ctaatgaata cggtgcggca ctgcgaccgg ctaattttac
     2581 tagcggttgc ggttatcgga taggtcttac agaaatcggt agcctgttgg gcacgggtaa
     2641 gcgcagccga agccggctcc ggagtgaata gcaccggcat accgaatgcg atcatcagca
     2701 ctataaacat gcgtatgtgg cgtttcatta cgcctattct aggcttgcaa ggacactact
     2761 gtcaaaattg ttatgtttat tcgtgcattc tcggacaaaa tctattacac ttatgcttat
     2821 gaagcggagt cggcccagga tactgttcgc cctcatcggc tatgtgtttg ccatggctgc
     2881 aagctttctt attcccgtgc tcagcaaccc ggttcaggcc ggcgaacccg tacagccatt
     2941 aaatattgtc ttcttttata atgagacgga caaagaggct attaaaagtg ggttggccgc
     3001 catcggcagc ccgattaccg ggggtccggg cgcaaatgcg aatatagcta agacggctat
     3061 ttacgcaaag gggagtaatt ttggcaccaa ccccttcctg ttccgttact cgggaaacac
     3121 aagctccgca gttgggggtt caataagacc aatttatgag gctacctatt tctgcgtagg
     3181 aggaagcgct aacccgcgca ttgcaacggc cggaggggcc ggtgatgcga aggttatact
     3241 tcacataatt ccgagtctaa ctgccgcctt tattgatgca agtcccacca gtccgcgaag
     3301 cgttaccggg gcgatgtctc tcgtaagtgc agagcaaaat ggatcaccgc gaacaatggc
     3361 ccaactccct tcagattgtc gcattaatgg ccttccccgc accgtcacca taccaaacta
     3421 caacctatta acagcagcca cgaaatccac ctggggaccc aaccttaaga cctcggggca
     3481 ggccgacgcg gccaccaacg ctccggccaa caatcaggct ggcaccgcaa gtgccggggt
     3541 agatgaagac gccgccagcc aatgcccaag atcggcggcc ggattgggct ggattgtctg
     3601 tccgtttatt ggcatcgtaa acgatttgtt cgcccgatca ttccaaaagt tcattgtcgg
     3661 cttaatgcac atagatccct tggatacgag cagcaccagc accaatttat acggtgtatg
     3721 gaaaaatatt cgcgaccttg caaacgtcct attcttgctc atctttatga taattgtgct
     3781 ggccaacaca ctttcattta acgttcaggc ctacactatt aaaaagatga ttccgaagct
     3841 cgtcgcggcg gcaattatgg ttcagttctc atttattatt tcggcggtct tgattgatat
     3901 atttaatatt attggcggcg gactggggga cttgctttcg agcttagcga atattccggg
     3961 cgatcagggg ggcagcttcc tggagaactt gtttgctacg gtattctcgg cgttgatttt
     4021 ggtacttggc ggtattatcg gtgtagtggc agtcggctct gccatcggga ccacgggagc
     4081 cttcgccgga gtagcaatcg gttttattat cgtgcttgct cttaccgcct tcttcgcctt
     4141 tatgtcattg ctctttagct tgatgttccg caaattaatt atcgtgatgc taatccttat
     4201 gtcgccgctg gcatttgcgg ccctagtact gcctagtacc gaaaaaatct ttaaggtatg
     4261 gataggcact tttatccaaa ttttaatgat gtatccaatt atcgtttttc tgtttgcctc
     4321 ggcaacggtt ctacagcaag ccctgggcag ctccgccaat gatgtaaacc cctctgtggg
     4381 aatccctgaa cttatggctg ccgttatgcc catagtagtt tgctttatgg ttccgatgac
     4441 ctttaagtgg ggcggcaaac taatgagcgc tggctcgaat ggcatcgaca gcctggtgcg
     4501 cggcaagatt ggcggttcgg cggtaagcaa gtttaaggat agcgaagcct acaaggttcc
     4561 ccgggcgcta cgggctagca ataagctaga aaagaagcaa ggccaagcaa gcatgtcggg
     4621 caccctcaag ggaatgaata agttcaatcg ctggaaagcg ggtgtgcagt tgcgtggcga
     4681 ctttctgggc ggcaaccaga tgattgccgt agagagaggt aaattcggac tgcctacagg
     4741 ccttccgaag cgtggggact acggagacat gcgtcgaatt caaatacgtc agcgcaacga
     4801 atatttggac aaaggtctgg ccaatgcagc gcttgaccgt cagcatctaa ttagtctgct
     4861 tgatccaatg actaaaggtg cgaaagacgg caattttgat gaagttatga aacaaccgct
     4921 tctttacgat caggtattga acggcctggg tagttacgtt aaaaaagaag gaaagctacc
     4981 atatgccgat gctttagttg atctaacagc catgggaaca aagatgggca aaaccgacaa
     5041 agaccttaga gaggaccaag actggatcat gggcattgat cacaacgcga caaaagcaat
     5101 gcctctggcg gccgagacaa cacatgtcac gaatcttgca aacaggcaag acattgatga
     5161 aactaccgga gatttaaaaa cggtgcatgt tgataacaat ccgaacctgg ctccacgcaa
     5221 ggtcaaggtc gcaagcacat cagaccatga atggcacgac ctagaagcat cattcggaca
     5281 agatactttt atgggcgata acaaggaaat cgccgttgac cagtttgtgc acgggctggt
     5341 tgagcaaagg gacgccaatg gaaacgttac ccagcatcgc gttattgaca aattgttagc
     5401 aaaagatcaa ctgctactcc acacactaca taccacggcc actcgatatg gcgctgtcaa
     5461 aaccgaggct agacaggcaa ttgttgatta tggcactagg gccctaacaa gactcgacgc
     5521 gctcgaggtc aaggcgcaaa ataacaacct gaatgccgaa gaggctgacg agctgaactt
     5581 tgccacccaa aataaattaa gggcagccta cacgtttgct gcccactttg ccgatgacgc
     5641 gaaagtctac caagataaat ttactgggaa cagcggagca accaaagacc ctgctctaga
     5701 gcaagccctt aaggacgttg gtggcgcgta gccatgggca cctataaagt tccacaaaat
     5761 gttgaggccg aagacaagat tctaggtccg ttgagcatga agcagtttat ttatgcgctc
     5821 atcggtttag ggtatgggct cttaacgttt gccattttaa agtcggtaat cctgttatgg
     5881 attctggttg gcctgccgcc ggccttggcg cttttggcat taggtcttta tcagcgccaa
     5941 gatcagtcga tggaaaccta tctcatttcc attgctcagt ttattggacg gccaaagtcc
     6001 cgggtgtggc agaaagagcc ggtagcggaa gtttttcatc ttgagccgcc gccgccaaaa
     6061 ccccgaatgg agacacggga ttcgcgtaag gtgcgcagtc aattggccca gctggccaac
     6121 gtggtagata cgcacggatg ggcaaccaaa cagcctcagg tccaagaagc cgaccagcaa
     6181 attcaggtgg ttgaccttaa tgagcggata ggcgacataa cgctgccagt tgagccgcgg
     6241 caagagatcc acatgccgga agttaccgat gccgatgaca tgctgaacat tagcggcgct
     6301 gctgcgcaaa atttggataa tcttattgca aatagcattc aaagcttgcg agctgatgtg
     6361 ctcgaaaaaa tggctgccgg cagcgctctc cccccaatgc caagcacaag cgggatgaca
     6421 acgccgccat ctggtgatat agtgaaatta gccgcggagg gtggagattt aacggtggcg
     6481 caacttgcct cacaagcccg acgccaaata gcagcaatag gtgaaggagc gccagcgagc
     6541 gtacaaaatg ccagtcccca accaaactaa tccagctcct aaaaagccaa gcactcaaga
     6601 caccctgctt attgcggaga tccgcgacgg tgttgtggta atgcgcgacg gctctattcg
     6661 cagcgtgctc atgggcagcg ccgttaactt cgaccttatg agcgctcaag aacgagaagg
     6721 ggtggagtac tcctaccaga gctttctaaa ctccctgcat ttccccattc aaatcctgat
     6781 taagtcacag cgcattgacc ttgacagcta tttgtctaac ctagcccagc tacgacagag
     6841 cgaagacaat ccgttgctcg gcgccttgat ggatgattat attttgaaca ttcaggggtt
     6901 gattgagcag gtaaatatta tggacaagca gttttttatt atcgtgccgt atcttccgcc
     6961 tttaatgtcg aaaatgaaaa agaagaatgt ggttacgggg ctggccggtg cttttaaaaa
     7021 gcaggcggta acaaccctca acgaaaatga ttttaaggca tttaaggatg agttggcgca
     7081 acgcgtcagc ctggtgtcgg ccggtttagg ccagattggc gtccgcaaca tcccgctaaa
     7141 cactcaagag ctgatcgact tattttattc ttcctataac ccggacgtag ctcaaaatca
     7201 aaagctggtc gacgctggac agctacaaac cgccgcggtt accaagggtg aaggcgatgc
     7261 tccgcaacac ttggccagcg gaggtccttt ctaatgggtc tattaggcgg caaaaaagaa
     7321 gtaaatgacc cgattgcgat tgctgaatct cagcgggcgc aggaacagca agaagctgag
     7381 gcggcatatc ggcagggaat tgtaacactg cgcgacttga ttgccccgcc aagctttagc
     7441 attgccaacg accacattca aattggcacc cgttatgccc gtacgttgta tgtatatggg
     7501 tatccgcgag aaatctttac cggatggctc agccctatta tcaaccttga cgaggttatg
     7561 gacatcagcc tcttcatata tccggttgag agccaagtgg tgctgaacaa ccttaagcgg
     7621 aaggtcggcc aaatggaagc cacctatgcc ataaaccaag aaaagggtcg ggtgcgtgat
     7681 cctggtcttg aggcggcttt aatggacgcc gaagaactgc gcgataagct tcaagtaggg
     7741 gaggagcggt tctttcgctt cggcctgtat gccaccgtgt acgccgatag catggaggag
     7801 ttaaaagata tccaacgtaa agtggaaggt attttcggcc agtccttggt gtacaccaag
     7861 cccgccagca tgcaaatgga gccagccttt aattcaaccc tgccgctggg aaccgatgag
     7921 ctgcaggtgg cccgcaacat gaatactgga gcactttcta ccagctttcc ctttacctcg
     7981 gccgaactta gccgtaacga aggcgtgctt tacggcatca accgccacaa taacggcctg
     8041 gtgctgttcg accggtttag cttggaaaat gccaacatgg tggtatttgc taaatccggc
     8101 gctggtaaaa gttttgccat taagctagag gcaatccgca gcctcatgct tggaaccgaa
     8161 atcctcatca ttgacccgga agacgagtac cattccatgt gcgaagcggt cggcggaagc
     8221 tacctgaggt taagtttagc ctcgtccagc cggctcaacc ctttcgacct gccgcgggtg
     8281 ctcgacagcg aggatgccga caatgccctg cgagccaata ttattacctt gcatgggttg
     8341 ttgcggctaa tgatgggcgg gcaggcttcg ccgctcagcc cagccgagga tgccgacttg
     8401 gacgttgcca ttattaacac ctattctaaa gcgggcatta cgaatgaccc gctaacccat
     8461 ggcgccacgc ctccaaccat gaacgacctc tacaaaacac tttcggaaat gagcggtaac
     8521 ggcccggcac ttgctcagcg cctgcgccaa tacaccagcg gcacctttgc cgggattttc
     8581 tctgagcagt caaatgtaga cttgaacaat aaatttgtgg tcttcaacat tcgcgacctg
     8641 gaagaagagt tgcgcccggt aggtatgtat atcgtcctga attatatttg gaataaggtt
     8701 aagtccgaca agcgaaaacg gattctagtg gtcgacgagg cttggcaact tatgaagttt
     8761 gaagatagcg ccaacttcat gttcagcatt gccaagcgcg cccgcaagta cttcttaggc
     8821 ctgaccacca ttacccagga cgttgacgac tttttaagct cgcggatggg ccgagcggta
     8881 gtgtctaact cgtcgctgca gctgctcctt aagcaagcgc cgagttcggt agatattatt
     8941 ggggagacat ttaagctgac cgccgaggaa aagacccggc ttaccaactt cccagtagga
     9001 gaggggttgt ttttcgctgg cctcaaccat gttgtcatga agattctggc ttcaaatgcc
     9061 gaaacgcagc taataacaac taatccccag gccattgccc agcaagaggc cgcagctaaa
     9121 gcctacagag aggcgcagca ggaaatcgca ggggcctaaa ccgtgcaaga cgacagcacc
     9181 gttaccgacg acaaagaggc tataggggac gagtctgctg ctgccgaaga acgacgccgg
     9241 gcaatggcgg cggcggcccg gcggcgcgat atgcgcagcc agcataagcg caaaaccgat
     9301 gacggggcca gccaacggcg caggggcacc aacgacctca acgacaaata tgggctgggt
     9361 ggtgatgact cgggaaagaa aaagctcagt aaagccaaga ccgcttatct ggccatgcga
     9421 gcagccgcgg gcagccgaac cgccatatcc agtttaattt tagggggcgg ttttatcgcg
     9481 gtagttattg ggctgttctt tatctacgct caatataagc tggttcacac tatggaggga
     9541 atggagcgcc attttttttc ccgcaacaac cacgctattc atgctcggca acaaattctg
     9601 atgcgcgaat ttatttttaa ccacctcgat ggagaaaacc tgccggacag caatgctaat
     9661 gcacccttcg ccgtgcaggt ttttaatact ctgtataagg gagatttcaa aaagcggcta
     9721 gaggcccaag gcgtaaaaat tacccgccgg aagagcctaa acggtctaac cactttggta
     9781 actattgagg atactcgaaa cggcaccaag cgaacctacg accagggaaa cggccaggac
     9841 cggcgggatc ttggactact gggagagcag ctgaacgacc gcctgtcggg agacggcacc
     9901 aaccgtattc gcgcggtagt cgtaaaggcc catttatcga gacgcttggg ctaccattgg
     9961 catccgttgg acccaatcag ggggagctat gctgaggtgc gcgctaaact ttttgcgcgc
    10021 atgctcaatc ctgcgttgcg tattccagcg ctccagtcta ttgcgtctga ggtgctaaaa
    10081 aagttttttg gcgacaccga cgagggaagg gaaatacagg agaatctttc agcggccatt
    10141 gactcgcccg acagggccgc cggaaaggta gcgctcagtg cggtaatcac agagacaacc
    10201 gctaaattcg taaagaatgc caatttggtt ggtctggcca ttttggcagc tgatgtaggt
    10261 tgtagaattt ctgattttct cgctgaccgt accgccagaa aactggtgca gaaaaagatt
    10321 gagctcgagc aaatgggcgt cttcagagat cttcgaagtg ccgccgacca gcaaaaggac
    10381 ggcaagaaca ctgatgccaa tactgtttcg gccgccggcg agcttctggc ctataccgac
    10441 aaagacggta aagttcatga tatatcggag tcaaacaact actcccgtca aaatggccgg
    10501 ccggtggagt accatccatc tacaaattgc gaggacaatc gagaactttg tcttcaaaaa
    10561 caagccagca aggcgatgcg cgctacaacg ctgggatcaa tatttgaggg ggcgggagac
    10621 ctgctcaaca gcccgttcta taaagccctg ccgctgccat tgcccggtac aacctcaacc
    10681 gtcgtcggct gcaccgcctt gcggccggtc ctgaatgttg tagacagcct tttcggcttg
    10741 attggcagag gggtggatac tgtgctttcc aacaccccgg gagtaaacac cctttggagc
    10801 agcataaaag atttggctgg cgacttcatt accatggtca gtaataaatt tattggcgca
    10861 ctggctggcc cctcaatcga catagcaacc acccgcgggc cagcgctgct taatgtggct
    10921 ggggctgggg ccagcttggc ggccggatca atagccgggt cgccggccgg aaccgacgag
    10981 acactcgatg aaaacgtctg ctacaacaag acgccagagg agaaaaagca agataccagc
    11041 ctttgtggca aaaagcttac tcgcgcgcag ctggttataa tcgacaatgc gattgtgcaa
    11101 gaaaaccaca ggcagtttaa tgagtcatcg ttatggacta ggatggcttc gctagacact
    11161 ccttattcat ttgccagccg catcgccttt gctacgccgt cgtctccaaa agtgctgatg
    11221 cagcgagccg ggactacact cactgccgcc atcaacccct ccggctggat gcgctttttc
    11281 gaattgcttc ccaatttatt cacacccaag ctgcaggcag taggctcagt ccccgggtcg
    11341 tccaagattg tagactccga ggggaaagat caatttggga cagacattta cggatttgag
    11401 gaagcagacg tacaaagctc cggatttgac actaagccgc atcttcataa tttggacact
    11461 gcgtctggct gcagcgctat ttctgactac gtggaaaaga agggacggga c
//
LOCUS       DASVGB010000037         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1091889, whole genome shotgun sequence.
ACCESSION   DASVGB010000037 DASVGB010000000
VERSION     DASVGB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091889"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VF272_01135"
     CDS             <1..148
                     /locus_tag="VF272_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014215718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7259517.1"
                     /translation="KAKIASVVFDRGSRIYHGRLHALAEAARAKGLEFYWLSHNNRRS
                     LKKR"
     gene            106..627
                     /gene="rpsE"
                     /locus_tag="VF272_01140"
     CDS             106..627
                     /gene="rpsE"
                     /locus_tag="VF272_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7259518.1"
                     /translation="MAIAQQPKEFEEKVISIDRVARVVKGGRRFRFRATVVIGDGKGR
                     VGLGIGKGGEVMTAIAKAISRARRNMITIPLKNGTIPHEITVRFSGAQVMLKPASTGT
                     GVIAGGAVRNVVEVAGIHDLLTKSFGSTNKVNNAYATIMALSRVKPISSSPLARIKPK
                     VSKSSAKAEKVAV"
     gene            636..1118
                     /gene="rplO"
                     /locus_tag="VF272_01145"
     CDS             636..1118
                     /gene="rplO"
                     /locus_tag="VF272_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7259519.1"
                     /translation="MKIHELTVTSTKNSKRVGRGIGSGKGKTAGRGTKGQNSRSGGGV
                     RPGFEGGQNPLAKRLPKKRGFVSLSTVNYLTVSLGDLNRVNGTTVTNAILAEQNLIKN
                     TFDPVKVLGSGELTKKLSITLQAATKDAVSAIEEAGGTFTVIAPSKRPKKPSKRNRTT
                     "
     gene            1136..2449
                     /gene="secY"
                     /locus_tag="VF272_01150"
     CDS             1136..2449
                     /gene="secY"
                     /locus_tag="VF272_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7259520.1"
                     /translation="MNLQTLRNAWGSKELRNRIFVVVGILLIFRLLSYVPVPVPDNQA
                     LANFLKQLFGSSQLLGFANLFSGGSLSNFSIIMMGLGPYINASIIIQLLTKVIPHLES
                     LSKEGDRGRKKINQYTRLLTVPLAVVQAIGMVVLIRQSSIQATQTDLIGNASLFQWFL
                     MVVTMTAGTIFLMWLGELITEKGIGNGISLIIFAGIIAALPSSATQILSLASNDTGRI
                     LQLLFFAVALFAVIYFLVMLNEGKRSIPVSYARRARGNRIYSGVDTYLPIRLITAGVI
                     PIIFALAFLAVPAFIGQLLSGASTPWVAAFAQWLTTFFATTSIGYPISYFLLVVGFTY
                     FYTGIIFNPKDISENMQKQGGFIPGIRPGNETAAYLTKVVNRLTLIGSIALGLVAVLP
                     FIVERFTNTQVLTLGGTGLLIVVVVALETMKQLESRAIQVSYDKY"
     gene            2452..>2647
                     /locus_tag="VF272_01155"
     CDS             2452..>2647
                     /locus_tag="VF272_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259521.1"
                     /translation="MAQPIFFISSSKAKFDAIKPVISQLQQIELDLPEIQELDAHKVI
                     AAKVQQAFKHHKGPILVDDTS"
BASE COUNT          667 a          634 c          650 g          696 t
ORIGIN      
        1 aaaagcaaag atcgccagcg ttgtgttcga tcgaggcagt cgtatttatc atggacgttt
       61 acatgcgttg gccgaagctg cccgagctaa aggattggag ttttattggc tatcgcacaa
      121 caaccgaagg agtttgaaga aaaggtaatc tcgatcgacc gcgttgctcg ggtcgttaag
      181 ggtggtcgtc gattccgctt tcgggcaacg gtagttattg gcgacggcaa aggccgtgtt
      241 ggactgggca tcggcaaagg cggcgaggtt atgacggcca ttgctaaagc gatttctcgt
      301 gcccgtcgta acatgattac cattccttta aagaatggaa ccatcccgca tgagataacg
      361 gtgcgttttt ccggggcaca agttatgctg aaaccagcat cgaccggaac cggcgttatt
      421 gccggcgggg cggtacgcaa tgtggttgag gttgccggca ttcacgatct gttgaccaaa
      481 tcctttggtt caactaataa agtcaacaat gcctatgcta caattatggc gttatcgcgg
      541 gttaaaccga ttagcagcag cccgttagcc cgcattaaac ctaaggtttc caaaagttca
      601 gctaaagctg aaaaagtagc tgtttaagga atagtatgaa aattcatgaa ctaacagtta
      661 catccaccaa aaacagtaaa cgcgtaggtc gtggtattgg ctccggtaaa gggaaaaccg
      721 ccggccgcgg cactaagggg cagaactccc gttccggcgg cggcgtacgg cccggttttg
      781 aaggcggcca aaacccgctt gccaagcgat tgccgaagaa acgaggattt gtttcgctga
      841 gcaccgttaa ttacttaacc gtttccttgg gggacctcaa ccgagtgaat ggcactacgg
      901 ttaccaatgc tattttggca gaacaaaacc ttataaaaaa cacgtttgat ccggtaaaag
      961 ttttaggtag tggagagcta accaaaaaac tcagtatcac gctccaagct gccactaaag
     1021 acgctgtatc ggccattgaa gaggccggcg gtacttttac cgttatcgct ccctccaagc
     1081 ggcctaaaaa gccctccaag cgtaaccgta cgacttaaag aacagcaggg gttagatgaa
     1141 cttacaaaca ttgcgaaacg cctggggatc caaagagcta cggaaccgca tatttgtggt
     1201 tgttggaatt cttttaatct ttcgcttgct ttcgtatgtg ccggtaccgg tacccgacaa
     1261 ccaagccttg gcaaactttt taaagcagct tttcggctca agccagttgc tgggatttgc
     1321 aaaccttttt tctggaggca gcttaagtaa cttctcgatt attatgatgg gtctcggtcc
     1381 ctacattaat gcgtcaatca tcattcagct tctaaccaaa gttattcctc atttggagtc
     1441 tttaagcaag gaaggcgacc gcggccgcaa gaaaatcaac cagtataccc ggttgctgac
     1501 ggttccgttg gcggtcgttc aggccattgg gatggttgtg cttattaggc aaagttcaat
     1561 tcaagcaacc caaaccgacc ttattgggaa tgccagtcta ttccaatggt tcttaatggt
     1621 ggtcacgatg acggcgggca cgattttcct gatgtggctt ggcgagctta tcaccgaaaa
     1681 aggcatcggc aacggtatct cactcattat ttttgccggt attattgcgg cattgccatc
     1741 aagcgcgacc cagatcttaa gcttagcctc aaacgatacc ggcagaatct tgcagcttct
     1801 cttcttcgcg gtagccctat ttgccgtcat atacttcctg gttatgctaa atgaaggcaa
     1861 gcgctccata ccggtctcct atgcccgccg tgcccgcggt aatcggattt attccggagt
     1921 tgatacctat ctgccaattc gtttgattac cgccggggtt atcccgatta tctttgccct
     1981 agcctttttg gcggttccgg cgtttattgg gcagctgcta tcgggcgcaa gcacgccctg
     2041 ggtagctgct tttgctcaat ggctcacaac ctttttcgct accacgagca tagggtaccc
     2101 gatttcatac tttttgctgg ttgtcggttt tacctacttc tacaccggta ttatcttcaa
     2161 tcccaaggat atctccgaaa atatgcagaa gcagggcggg tttatcccag gcattcggcc
     2221 aggcaatgag accgcagcct atttgaccaa agtagtgaat cggctaacgt tgatcggatc
     2281 aattgctcta ggtctggtag cggtcttgcc gttcattgtg gaacgcttta ctaataccca
     2341 ggttttaacg cttggtggca ccggcttgtt aattgtagta gtggtggctc ttgaaacgat
     2401 gaaacaactt gaatcgcggg caatccaagt aagctacgac aagtattagc cttggctcag
     2461 ccaatttttt tcatctccag cagtaaggct aagtttgatg ccatcaaacc agttatttcc
     2521 caactccagc aaattgaact ggatcttccg gaaattcaag aattggatgc gcataaggtg
     2581 atagctgcca aggtacagca agcgtttaag catcacaaag ggcccatact ggtagatgac
     2641 acatcat
//
LOCUS       DASVGB010000038         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_767236, whole genome shotgun sequence.
ACCESSION   DASVGB010000038 DASVGB010000000
VERSION     DASVGB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767236"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF272_01160"
     CDS             complement(<1..222)
                     /locus_tag="VF272_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259522.1"
                     /translation="MLRYLKLILVSFGLVIVLLFPGAGQAAVISSESGSSEVSANQTV
                     DDDLYISGEQVVIDGTVNGDIYAAGSSISI"
     gene            complement(292..1017)
                     /locus_tag="VF272_01165"
     CDS             complement(292..1017)
                     /locus_tag="VF272_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX7259523.1"
                     /translation="MKDTKEVVILHLYPEELNMYGDRGNILTLVQRLKWRGYQPIIRT
                     AGVDDQVDIAKADIIFGGGGQDRGQLAVGKDLQRHAAALRNAAAGGVPMLVICGTYQL
                     FGHGFTTLEGTVIPGISIFQANTIGSSRRMIGNAIIESSMGRLVGFENHSGRTLLKPG
                     QPSLGTVSQGFGNDGSSGNEGAVTNQVYGTYLHGPVLPKNPVLADHLIALALERKFGI
                     QDLEPLDDELEKAAAAQAASRPQ"
     gene            complement(1014..2381)
                     /locus_tag="VF272_01170"
     CDS             complement(1014..2381)
                     /locus_tag="VF272_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7259524.1"
                     /translation="MPVVATIAIGKLIIFFSRLLGRGGGSALPGLVAERIDPQIGHKL
                     AKNIPHGSIIVTGTNGKTTTSKMLAKILTDGGESLVVNTAGSNLSRGVVSALIEHTGI
                     GGKFRQTTGLFEVDEAAMPAVCALVQPRAIVVLNIFRDQLDRYGELDTTAKLIGKGIA
                     STEATLFLNADDPLVASLASFAKPDKTHNFGIDDAPADKLSHDVTADSHICPECGRSL
                     IYTQNFYGHIGHYSCTEGHFDRPKPDYWASGIKLSVQSAEALIKAPKQEIPMTLQLPG
                     LYNIYNALAAASVAFDAGLSAIAISSSLGSVRAAFGRVEKITIGGKEIYLLLVKNPTG
                     FNQIIQTFLMQKQQQNILLCINDNFADGRDVSWLWDVAFEELVPHNHTIAVSGTRASD
                     MALRLKYAGMAAARSEVQLDEALNQFITQLPAGGTGYLIPTYTAMLALRKHLSKMTEL
                     PEVWR"
     gene            complement(2391..>3174)
                     /locus_tag="VF272_01175"
     CDS             complement(2391..>3174)
                     /locus_tag="VF272_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7259525.1"
                     /translation="TFNHWYIIFWSQLLRLGVLAAVFLIFSWELRISWLSLTLAVLSS
                     GIFLLGLRFTFHALKVGPVSLASPFIGSYAFPAVLLSLVFLGEQLQYSQLVAVVLLIF
                     GAILISVSSRTHLPQRNFWTQPVVRLALIGAICIGTAVFFLAQLVHLAGWQTAIMLQT
                     IVLIVGSGIGLWLTGVALLPANRKPLNGAIIGLSAFGLMGALMLSYGFSVSLVAIVAP
                     VASTSPLITVGLSLYFFKERLSRYQLVGILVVVLALSLLAVR"
BASE COUNT          827 a          860 c          772 g          715 t
ORIGIN      
        1 aatactgatt gaagaaccgg cagcatagat atcgccatta acggtcccat caatcactac
       61 ctgctcgccg cttatataga ggtcgtcgtc gacggtttga ttcgcactta cctctgaact
      121 accactctcc gaactaataa cagctgcctg tccggcgccg ggaaacagga gcacgataac
      181 caggccaaag cttacgagta tcaattttag gtagcgcagc atctgacctc cttggtaata
      241 gtcttagtat gtcccttggc agagccaatt acaagcatga ccgtgtcgac attactgagg
      301 gcggctagcg gcctgggcgg cggcggcctt ttccagctca tcatcaagcg gctcaaggtc
      361 ttgaatgcca aatttacgct ccaaggctag ggctataaga tggtcggcca gcaccggatt
      421 cttcggtaaa accggaccat gcaaataggt cccgtagact tggttggtaa cggcgccctc
      481 attcccgctg ctgccatcgt tgccgaatcc ctgtgaaacc gtgcctagcg aaggctggcc
      541 cggcttcaat agtgtacgac cgctatggtt ctcaaacccg accagccggc ccatggatga
      601 ctcgattatc gcattgccga tcatccgccg agacgaacct atggtgttgg cttggaagat
      661 tgagatacca ggaataaccg ttccctccaa ggtcgtaaag ccatgtccaa agagctgata
      721 ggttccgcaa attactagca taggcacccc gcccgctgcc gcattgcgca gcgcggcagc
      781 gtgacgctgc aagtccttcc ctacggctaa ctggcctcgg tcctggccgc cgccgccaaa
      841 aataatatcg gctttggcta tatccacctg atcatcgacc ccggcagtgc gaataatcgg
      901 ctggtagccg cgccacttca ggcgctgtac caaggtaaga atatttccgc gatctccata
      961 catattaagc tcttccgggt ataaatgcag gatgactact tccttcgtgt ctttcatcgc
     1021 cacacctccg gtaattccgt cattttactt aaatgtttgc gcaatgccag catcgccgta
     1081 taggtcggta tcagatagcc ggtcccgcca gcaggaagtt gagtaataaa ctggttcaat
     1141 gcttcatcca gctgcacttc ggatcgtgca gctgccattc cggcatactt cagccgcaag
     1201 gccatatcgc tggcacgcgt tccggaaacc gcaatggtat gattgtgcgg tacgagctct
     1261 tcaaaggcta cgtcccataa ccacgataca tcgcggccgt cagcaaagtt atcattgata
     1321 cagagcaaga tattctgttg ttgtttttgc atcaggaagg tttgaataat ttgattaaag
     1381 ccagtgggat tttttacaag caggagatat atctccttac cgcctatggt tattttttca
     1441 acccggccga acgcggcgcg tacactgcca agcgaactgc taatcgctat tgcgctcaat
     1501 cccgcatcaa aagccacgct agcggccgct agggcgttgt aaatgttata gagaccggga
     1561 agctgcaagg tcatgggtat ctcctgcttg ggtgctttaa ttagggcctc tgccgactgg
     1621 acactaagct tgatcccgct tgcccagtag tctggcttgg gacggtcaaa gtggccttct
     1681 gtacaactat agtgtccgat atgcccataa aaattttggg tgtatattag cgaacggccg
     1741 cattctggac agatatgcga gtcggctgtc acatcatgag aaagcttatc agcgggcgca
     1801 tcgtcaatgc cgaaattatg ggttttatca ggtttagcaa agctggccag actggcgacc
     1861 aacggatcgt cagcatttaa aaacagggtg gcctcggtag aggcaatccc cttgcctatc
     1921 aacttagcgg tggtatcgag ttcaccgtag cggtctaatt ggtctcgaaa aatgttcaac
     1981 accacaatcg cccggggttg aaccaatgca cacactgccg gcatggcagc ctcatcaact
     2041 tcgaacaatc ccgtggtctg gcggaatttt ccaccaatac cggtgtgctc aattaaggcc
     2101 gaaaccacgc cgcggcttaa atttgacccg gcggtattaa ccactaggct ctccccgcca
     2161 tcagttaaaa tcttggccaa catttttgag gtagtcgtct tgccgttggt gccggtaaca
     2221 ataatgcttc catgaggaat attcttggcc agcttgtggc caatctgagg atcgatgcgt
     2281 tctgcaacta ggccgggcaa cgccgagccg cccccgcggc caagcaggcg gctgaaaaag
     2341 ataatgagct ttccgatggc aatggtggct acaactggca taggcttatt ctaccttacc
     2401 gctaacaaac tgagggctag tacaactacc agaataccaa ccaactggta gcgggaaagg
     2461 cgctccttaa agaagtacaa tgatagtccg accgtaatca agggtgaggt tgacgcgacc
     2521 ggagccacaa tagccaccaa cgacacagaa aagccgtagc ttaacatcaa cgcccccatc
     2581 aacccaaacg ctgacaatcc tatgatggcg ccgtttagag gcttgcggtt agcaggaagc
     2641 aacgcaactc cggtcaacca aagtccgatg cctgatccaa caataagcac gattgtttgt
     2701 agcattatgg cagtctgcca ccccgctaga tgcacaagtt gggcgaggaa aaacaccgcc
     2761 gtaccaatac atatcgcccc gataagtgcc aggcgcacaa ccggctgagt ccaaaagttt
     2821 ctctgcggaa gatgagtccg gctgctgact gagattaaga ttgcaccaaa tatcaggagc
     2881 acgacagcca ccaactggct gtattgaagc tgctcaccta aaaagaccaa ggaaagtagt
     2941 accgccggaa aggcataact gccaataaat ggactggcca agctgactgg cccgacctta
     3001 agtgcatgaa acgtgaatcg caggcctaaa agaaatattc cgctgctaag cacggcgagt
     3061 gtcagactta accagcttat tctcaactcc cagctaaaaa taagaaatac tgcggcaagg
     3121 acacctaacc gcagcaactg gctccaaaat atgatgtacc aatggttaaa cgta
//
LOCUS       DASVGB010000039         8988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_752334, whole genome shotgun sequence.
ACCESSION   DASVGB010000039 DASVGB010000000
VERSION     DASVGB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752334"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF272_01180"
     CDS             complement(<1..186)
                     /locus_tag="VF272_01180"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017311919.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7259526.1"
                     /translation="MTNRELHEKCAVFAIYEPDQQVAHKVYYGLYALQHRGQESSGIV
                     TTDGKRFYRHRDQGLVAQ"
     gene            complement(233..826)
                     /gene="nadD"
                     /locus_tag="VF272_01185"
     CDS             complement(233..826)
                     /gene="nadD"
                     /locus_tag="VF272_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEX7259527.1"
                     /translation="MNRIGLISGTFDPIHNGHIALATHAMDQLGLDEVLLLIDADPPH
                     KPDVSDFEHRMAMAQLATQGYPRISPDKYALQRRTKNHTIATARELHEANPASELVLL
                     MGADTFLSIDRWADREELFKLLSFGMAGRNGSDSLEVDALKWRLGDSAQALQCEVVTM
                     PLTNISSSAIRTSVGLNEAISGLHPAVYDYIKNNRLY"
     gene            complement(823..1545)
                     /locus_tag="VF272_01190"
     CDS             complement(823..1545)
                     /locus_tag="VF272_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7259528.1"
                     /translation="MGDEKKGDFSGLKALFINATLKKSPDPSNTDGLVAISRGIMEEQ
                     GVEVDHIRSIDHDIAQGVYPDMRAHGWATDEWPELFKRVMAADILVLAGPIWLGDNSS
                     EMKKVIERLYANSSDLNEVGQYAYYGRAGGCLITGNEDGIKHCAMNILYSLQHLGYTI
                     PPQADAGWVGPAGPGPSYLDPGSGGPENDFTNRNTTFMTWNLMHMAKMIKDNDGIPAH
                     GNQRSKWTAGTRYDSENPEHRS"
     gene            1601..2476
                     /locus_tag="VF272_01195"
     CDS             1601..2476
                     /locus_tag="VF272_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7259529.1"
                     /translation="MNKLLKVALVQKKPPENQHDAQVRGEAYCREAASQNADIILFPE
                     LWSTGYAFPDPSDDKQTARWRQHAASRHSEFVSSFQSLAKELETAIAITYLEDSGRGQ
                     PKNSLTLFDRRGDEILHYSKVHTCDFGPELLTAAGNEFKTNLLSTTAGDIRVGAMICF
                     DREQPESARLLMLQGAEIILTPNACGLGRAQLQQFSTRAYENMVGMAMANYPAPRCNG
                     RSVAYSPIVVDENHDDVENNLVLAGPEEGIYYAEFDIEQIRAWREKQIWGNAYRHPEA
                     YGDITSNTINAPFTR"
     gene            complement(2535..3014)
                     /locus_tag="VF272_01200"
     CDS             complement(2535..3014)
                     /locus_tag="VF272_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259530.1"
                     /translation="MLGSTRFTSTHLVGIMLATAISLAFAPVVVEQVDGRNQAFVAQA
                     AYKMKYHEAHIPAIAETFDKCAKLQYMKVQNVPIKTYDCMAASIKVPCHVGFPFRSNV
                     GLCTPDFWMKNPLNYEKGDLHCKGFLYYKKTASGVRRQQKSWWRCKRGTYIPADRQA"
     gene            complement(3238..3323)
                     /locus_tag="VF272_01205"
     tRNA            complement(3238..3323)
                     /locus_tag="VF272_01205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3288..3290),aa:Leu,seq:cag)
     gene            complement(3376..5133)
                     /locus_tag="VF272_01210"
     CDS             complement(3376..5133)
                     /locus_tag="VF272_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7259531.1"
                     /translation="MITYIRFRLQRSLRRAQRNVRRYWHLGNRYLRFGFWGKWRQLTM
                     IRRFALGWWFVVLALGIGLFLQTGSIRQRGLVVQPLPGGSYSEAIVGTVKNLNPVLPE
                     GGASADANHLIFSGLTRFTAEGKLEPDIAESWDISADGKVYTFHLRKNIRFHDGVPAT
                     AQDVVFTLTAIQNPDTRSALAASWQGVKVSAPDDHTAVFTLPKAYTPFINATTVGLLP
                     RHLLENTEPRDLRVAGFNQKPVGTGPYKLTSFDVAAGEINLAANNDYHFSKPLISIIT
                     LRLYSDYDKALDAYKHRQVLGVGRLQPDQIDEATKLGTLKLHRASVPNAVAVFFKTTS
                     PVLADKAVRLGLAQATDRAAIVKDQFNNEASTLTGPLFATGLNLAGSAHQPAFNIQQA
                     RSTLEFAGWKPGRGGIRVKDGKQLKIRLLTQADSPYGPVARALAKQWKKAGAELQVIE
                     VGPEKLQQSYIRPRNYDALLYGINLGADPDIYAYWSSTQTKDPGLNISVYSSRQADTA
                     LESGRILRDPALRAAKYRSFIQTWVGDTPAVMLYTPSYEYGVDTSVYGVSVRKLINPS
                     DRFTGIENWSVKVREVPRR"
     gene            complement(5149..5370)
                     /gene="secG"
                     /locus_tag="VF272_01215"
     CDS             complement(5149..5370)
                     /gene="secG"
                     /locus_tag="VF272_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7259532.1"
                     /translation="MKSLFNYITIITSIIIITTVLLQSRGSSLGASMGGGDSEGYRTR
                     RGAEQGIYYTTIGAGFIFVLSVLLSILSK"
     gene            complement(5354..5719)
                     /locus_tag="VF272_01220"
     CDS             complement(5354..5719)
                     /locus_tag="VF272_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7259533.1"
                     /translation="MIRAFLLRWLINFLGLWAAANVLSGVDYGGNPGVLIIGALLFSV
                     VNAVIRPLLIVLALPAILLTLGLFTFIVNGFMLYLVTLIYPALQVTSFWQAVWAVLII
                     WIVNYLFTNIMEGSREKSV"
     gene            complement(5716..6096)
                     /locus_tag="VF272_01225"
     CDS             complement(5716..6096)
                     /locus_tag="VF272_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7259534.1"
                     /translation="MMKEPATIYVIDDDTALRTAYTAALSGMGYNVKSADDGIEGQKL
                     LAQAKPALIVLDMLMPNLDGMSFLKQLRSDHDNDAIKIVVVSNFEAMSEAIDLGATKY
                     MSKMQHTPDEVAAAVDSILKVSNA"
     gene            complement(6093..6479)
                     /locus_tag="VF272_01230"
     CDS             complement(6093..6479)
                     /locus_tag="VF272_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7259535.1"
                     /translation="MTPSKTSVLIVEDDQMLRSAYETVLTMDGYETSVAADGQEALAL
                     AKKIKPNVILLDMLMPTMNGLEFLEQFRAKTEHPTIKIIVFSNMSVPSDVQNALRHGA
                     CKYLTKSSFTPREIEDIIKEVLAEEE"
     gene            complement(6713..7630)
                     /locus_tag="VF272_01235"
     CDS             complement(6713..7630)
                     /locus_tag="VF272_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259536.1"
                     /translation="MKRNSRFYAALGLISLIYVLLSLSSPVADSARNFNATTFQFRAI
                     QISVIIPVIIIWSVALWGSLKFKRYAQNIIESQDGQALHSVANGLIALVFGLALNSMI
                     QAVQPLLRGSSYFNEWVIFANYLELAVVLVSFYMIYRGALKLVSLVQFKGFARAQAGV
                     ILALVGLGTLYAWLLAMNPYRQASPDITRIVPLYLPDWLIVVSIIVPYLLAWYMGLMA
                     AMSILLYQRNAPGIIYRRSARHLSFGMLAIATSSVLLQMLSAIGPSLSHLGLNSILLI
                     LYLLIIAYGFGHIMVAYGAKQLAKLEEAV"
     gene            complement(7627..>8988)
                     /locus_tag="VF272_01240"
     CDS             complement(7627..>8988)
                     /locus_tag="VF272_01240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX7259537.1"
                     /translation="DFMKASERHRVQKILRRVMKTGRSTELEYTTYINNSQQWYKNSV
                     GPIFTDGRITGLVLSTFDISQERLALKKLEEQKKAAEAAKIQAQTLLSSIGEGLLVID
                     EHGVIASVNSSAAAALGYDVKTLTGKWFPGTVLALDDQGKSIDPLNRPATQALMSGRS
                     ISETVWYQRKDGSVFPVAVTVSPVILDGRPVGAIEVFRDLTNERELERAKEEFVSLAS
                     HQLRTPATGAKAFISLLLDGYAGKLSERQKEYMQKIRKANERQLEIINDMLNVARIDS
                     GRIMPEMVSADIAEILEDIIEVHQPVAMERSQRLELILPSHRSAVVCDPKLIHMALEN
                     IVNNASKYTHKGGSIGVSLRFSENQAHVRVEDTGVGIAVEDQSKIFQRFARVYNPLSV
                     ARGGTGLGLYLTRNIVDMHQGRIGVDSKPGAGTIFDIELPLQPIGSLAQFADNQVTLG
                     REK"
BASE COUNT         2315 a         2331 c         2088 g         2254 t
ORIGIN      
        1 ctgggctacc agaccctggt cgcgatgacg gtaaaaccgc ttaccgtctg tagtaacaat
       61 gcccgagctt tcctggccgc ggtgttgcag cgcatacaac ccgtagtaaa ccttgtgggc
      121 aacttgttgg tccggctcat aaatggcaaa tactgcacat ttttcatgca gttcacggtt
      181 tgtcatagtt ttattctata atgttctaac actaaaagaa agcagaagcg ctttagtata
      241 agcgattatt ttttatatag tcgtaaaccg cggggtgcaa tccggatatt gcttcattaa
      301 gaccaacact ggtgcggatg gcggatgagg aaatattggt aagcggcatt gttactacct
      361 cgcattgcag tgcctgcgcc gaatctccca gccgccactt gagcgcatca acctccaaac
      421 tatcactccc gttgcgtccg gccataccaa aagacaatag cttgaacagt tcttcacggt
      481 cggcccaccg atcaattgat agaaaagtgt cggcacccat taacaacacc aattcgctag
      541 cgggattcgc ttcatgcagt tcgcgagccg tagcaatcgt atggttttta gtccggcgct
      601 gcaatgcata tttatcagga gaaatacgcg gatacccttg tgtcgccaac tgagccattg
      661 ccatccgatg ttcaaaatct gaaacatccg gcttgtgcgg ggggtcggca tcaatcaaaa
      721 gtaaaacttc atctaaccct aattgatcca tggcatgggt agccaaggca atgtgtccat
      781 tgtggatagg gtcaaatgtg ccggaaatca ggccgattcg gttcaggagc ggtgctccgg
      841 attttcagaa tcgtaacgag tcccagcggt ccactttgaa cgctgattgc catgggccgg
      901 tatgccgtcg ttatctttta tcattttcgc catgtgcatt aaattccagg tcataaaggt
      961 agtattgcga ttcgtaaaat cattttccgg tccgcctgaa cccggatcaa gatatgacgg
     1021 gccgggacca gccggcccca cccaaccggc atcagcctgg ggcggtatgg tgtagccaag
     1081 atgctgcaag ctatacaaaa tattcatagc acagtgctta atgccatctt cgttgccggt
     1141 aatcaagcaa ccgccggccc gaccgtaata ggcatactgg ccaacctcat ttaaatctga
     1201 tgagttggca tacagccgct caattacttt tttcatttcc gagctgttat cgcccagcca
     1261 aatcggcccc gccaacacta aaatatctgc agccataact cgcttaaaca gctccggcca
     1321 ctcgtcggtt gcccaaccat gcgctcgcat atccggatat acaccctgag caatgtcatg
     1381 atcaatcgat cggatatggt caacctcaac cccctgttcc tccatgatgc cgcggctgat
     1441 tgcgactaac ccgtcggtat tacttggatc cggtgatttt tttagcgtgg cattaataaa
     1501 gagggccttt agcccggaaa agtccccttt tttttcatcg cccataattg cagctccact
     1561 tcttgtatgt tgattatagc tcatgtatta cacttatggc atgaacaagc tattaaaggt
     1621 ggctctggta caaaagaagc cgccggaaaa tcaacacgat gcccaagttc ggggcgaggc
     1681 gtattgcagg gaggccgcta gccaaaacgc cgatatcata ttatttcccg agctatggag
     1741 caccggctac gccttccccg accctagcga cgacaaacaa accgcccgtt ggcgacaaca
     1801 tgccgcctct cgccacagtg agtttgtctc ctcatttcaa tccctagcaa aagagctgga
     1861 aacggctatt gcgatcacct acttagaaga cagtggccgt ggacaaccaa aaaattcatt
     1921 gacgctattt gatcgccggg gcgatgaaat cttgcactat tcaaaagtcc atacctgcga
     1981 tttcggccct gaactactaa ccgctgccgg caatgagttc aagactaacc tgttatctac
     2041 cactgccggc gatattcgag ttggggcgat gatctgcttt gaccgcgaac agccggaaag
     2101 tgcacgcttg ctgatgctac aaggagccga aatcattctc accccaaacg cctgcggtct
     2161 tggacgtgcg cagctccaac agttttcaac tcgtgcctat gaaaacatgg tgggcatggc
     2221 aatggctaac tatccagcac cgagatgtaa tggccggtct gttgcctata gcccaattgt
     2281 ggttgacgag aaccatgatg atgtcgaaaa taatttggtc ttagccggtc cggaagaagg
     2341 aatatattat gccgagtttg atattgagca aattagggcg tggcgagaaa aacaaatttg
     2401 gggaaacgcc taccggcacc ctgaagccta tggcgacata acatcaaata cgataaatgc
     2461 tccgtttact agataattgc tgaaaggctt cagaggagcg ggcgcgaacg cccgctcctc
     2521 tgtcacagag gttgctatgc ctgccgatcc gcggggatgt aagttcccct cttgcacctc
     2581 caccaggact tctgctggcg tcgaacgcct gaggccgtct tcttgtagta cagaaacccc
     2641 ttgcagtgaa ggtccccctt ctcgtagttg agggggttct tcatccagaa gtcaggcgtg
     2701 cagagcccga cattgctccg gaagggaaac ccgacatggc agggcacctt gatggatgcc
     2761 gccatacagt cataggtctt gatggggaca ttctggacct tcatgtactg cagcttggcg
     2821 cacttgtcga acgtctccgc aatcgccggg atgtgggcct cgtggtactt catcttgtac
     2881 gccgcctgag cgacgaaggc ctggttccga ccgtccacct gctccaccac gacgggcgcg
     2941 aatgcaagcg agatggcagt ggcgagcatg atccccacca ggtgtgtgct tgtgaatcgg
     3001 gtgcttccca acatggttcc caacctcctg cttgtgctac ggggcggatc cagcgaatta
     3061 tcacagcact cctggctgct aaaatctccg atcgaggcgg gtaagacctg cgcagattag
     3121 aaaatctacg tcacatacgg ttttaccata attatattat aaaatcaatt attattatcg
     3181 ggtgaatggc caaaaaataa caatattttt ggtaaatatt atattgtttt gattgtctgg
     3241 tgggcgagag aggacttgaa cctccacgag cttgcgctca ctagaacctg aatctagcgt
     3301 gtctaccatt tcaccactcg cccgcgaacc aggatgactg ataaactata gcttgaatca
     3361 aggctttttg gaagcctagc ggcgtggtac ttcgcgaacc tttaccgacc aattttctat
     3421 ccctgtgaag cgatcagaag gattgatgag tttgcgaact gacacgccat aaaccgaggt
     3481 atcaaccccg tattcatagc ttggcgtgta cagcattaca gccggcgtgt cgccaaccca
     3541 tgtctgaatg aagctgcggt atttggccgc acgcagggct gggtctcgca ggatgcgtcc
     3601 gctttctaac gccgtatcag cttggcgcga gctatacacc gaaatattca aaccgggatc
     3661 cttagtctga gttgagctcc aataggcata aatatcggga tcggcaccaa ggttgatgcc
     3721 gtatagtaaa gcgtcataat tacgggggcg aatataactc tgctgtagtt tttccggacc
     3781 aacttcaata acttgcagtt cagcgccggc ctttttccat tgcttggcta acgcccgagc
     3841 gacagggccg tagggactgt cggcctgggt cagtagccgg atcttcaact gtttgccatc
     3901 cttgacccgg atgccgcctc gtcccggttt ccaccctgca aattcaagtg tactgcgtgc
     3961 ctgctggata ttaaatgccg gctgatgggc ggatcctgcc aaatttagac cagtagcaaa
     4021 cagcggaccg gtcaaggtag acgcttcatt attaaattga tccttcacaa tagcagcccg
     4081 atcggttgct tgggccagac ccaaccgcac cgctttatct gctaacaccg gagaagtagt
     4141 tttaaagaaa accgccaccg cattgggaac gcttgcccga tggagtttta aggtgcccag
     4201 tttggttgcc tcgtcaattt ggtcgggctg taaccgaccg acgccaagaa cctggcgatg
     4261 cttataggca tcgagagctt tgtcgtagtc gctatagagg cgcagggtga tgatggaaat
     4321 caacggttta ctaaaatgat aatcgttatt agcagccaga ttaatttctc cagccgccac
     4381 atcaaagcta gttaacttgt acgggccggt gcccaccggc ttctgattaa atcccgccac
     4441 gcgtaaatcc ctaggctcgg tattttccaa aagatgacgc ggtaatagtc caacggtcgt
     4501 cgcattaata aagggcgtgt aggccttggg caaggtaaat accgccgtat gatcgtccgg
     4561 tgcgctcacc tttaccccct gccagctggc cgccaaagcc gaccgggtat ccggattctg
     4621 gatggccgta agggtaaaaa ccacatcttg cgcggttgct ggaaccccgt cgtgaaaacg
     4681 aatgttttta cgcaaatgaa acgtatatac ttttccgtct gcactaatat cccagctctc
     4741 ggcaatatcc ggctcaagct tgccttcagc cgtaaaccgg gtgagcccgc taaatattaa
     4801 atgatttgca tcggcgctag ccccgccctc aggcagaacc gggttaagat ttttaaccgt
     4861 gccgacgatc gcttcactgt agctgcctcc aggaagcggc tgcaccacca gtccgcgttg
     4921 gcgaatggaa ccggtttgta aaaataagcc tatgccaagc gccagaacca caaaccacca
     4981 gcccagtgcg aatctccgaa tcatagttag ctggcgccac tttccccaaa aaccaaaccg
     5041 caggtagcgg ttgcccaggt gccagtaccg ccttacattg cgctgggctc ggcgtaagct
     5101 gcgctgcagg cgaaaacgga tgtaggtaat catactatta ctttaccctt acttggataa
     5161 aatactcagc aataccgata atacaaaaat aaagccggcg ccaatcgtcg tgtaataaat
     5221 cccctgttca gcaccccgac gggtgcgata tccttcacta tcgccgcccc ccattgatgc
     5281 tcctaagctg gaaccgcgag attgcaacaa cacggtcgta ataatgataa tcgacgtaat
     5341 aatcgttatg tagttaaaca gacttttcac gagaaccttc catgatgttg gtaaataaat
     5401 agttcacgat ccagataata agcacggccc acactgcttg ccaaaaggat gtaacttgca
     5461 aggccggata aatcagcgtc acaaggtaca gcataaaccc gtttactata aaggtgaaga
     5521 gacccagagt caacaagatt gccggcagcg ccaatactat gaggagcggc cgaataaccg
     5581 cattcacaac cgaaaatagc aatgcgccga taatgaggac gccgggattt ccgccataat
     5641 ctacgccgct aaggacattc gcggctgccc acaacccaag aaaatttatc agccagcgta
     5701 acaagaatgc acggatcatg catttgaaac ttttagaatg ctatcgactg cagccgctac
     5761 ttcatctggt gtatgctgca tttttgacat atattttgtt gccccaagat caattgcttc
     5821 ggacatggct tcaaagttac tgactactac gatcttaatg gcatcattat catggtcgct
     5881 tcgcagctgt tttaaaaagc tcatgccatc taaattgggc atgagcatat caagtacgat
     5941 caacgccggc tttgcttggg ctaatagttt ttggccttca atcccgtcat ctgctgattt
     6001 aacattatat cccattccac tgagtgctgc cgtgtaggcg gtccgcagcg cagtgtcatc
     6061 atcaattaca taaattgttg ctggctcttt catcattctt cttcagctaa aacttcttta
     6121 ataatatctt cgatttctcg tggcgtaaaa cttgattttg ttaaatactt gcaggcgcca
     6181 tgtcttaatg cattttgaac atcactagga accgacatat tgctaaacac aattatcttg
     6241 atagtggggt gttcggtctt cgccctaaac tgctctaaaa attccaaccc gttcatagtg
     6301 ggcatgagca tatcaagcaa aattacatta ggtttgattt tcttagccaa ggctaatgcc
     6361 tcctggccgt cagcggcaac cgatgtttca taaccgtcca tcgttagaac ggtttcgtac
     6421 gcgctgcgca gcatctgatc gtcttcaact attagtacgg aggtttttga aggggtcatg
     6481 taagttgggt tagtttgcgg ctgcattcat cgatgtcgga tctattctca gttaacaggc
     6541 ccatagacac ttgaatccat tctattaatt ggggcatatc tgcccggtct atttcgtgag
     6601 gttccttgtt taaatgagac tgaatttgcc gtttcacaaa gcgctcagaa gccgggccaa
     6661 gataatactc aacaatagtc actatagcat catagaggcg agtcgtcata cgttaaaccg
     6721 cctcctcaag cttagccagc tgcttggctc cataagccac cattatgtgc ccgaagccat
     6781 aagcaataat tagcaggtag agaatgagta agatgctgtt gagcccaaga tggcttagcg
     6841 aaggcccaat cgcactcagc atttggagta acaccgaaga agtcgcaatc gccaacatac
     6901 cgaaggatag atgtctggct gagcggcggt atataatgcc gggcgcgtta cgctgataca
     6961 acaagatact cattgcagcc atgagaccca tataccacgc cagtaaatag ggaacaatta
     7021 tgctgactac aatcagccaa tcgggaagat aaagcgggac aattctggta atatccggag
     7081 atgcctggcg atagggattc attgccaata accaggcata aagcgtgccg agcccaacga
     7141 gcgccagaat aactcctgct tgagctctag caaacccctt aaactgcacg agcgagacaa
     7201 gcttcaaggc tccacgataa atcatgtaaa atgacaccag taccaccgcc agctcaagat
     7261 aattggcaaa aatcacccat tcgttgaagt atgacgaccc cctcagcaac ggctgcaccg
     7321 cctgtatcat agagtttagg gccaagccaa ataccagcgc aataaggcca tttgcaacgc
     7381 tatgcaacgc ctggccatcc tggctttcaa taatgttttg agcatagcgc ttgaatttta
     7441 aagaccccca caaagcaaca gaccaaatta taatcacggg aataatgacc gatatctgta
     7501 tggctcggaa ctgaaatgtt gtggcgttaa agttgcgagc cgaatccgct acgggagatg
     7561 acaagctgag aaggacatag ataagcgaaa taagacccag tgcggcatag aaacgcgaat
     7621 ttcttttcat ttttctctcc cgagtgttac ttgattatcc gcaaattgag ccagcgaacc
     7681 aataggctgt agcggcagct caatatcaaa tatcgtacca gcccctggtt ttgagtcaac
     7741 gccaattcga ccttgatgca tatcgacaat attgcgagtc agatataatc ccaggccggt
     7801 tccgcctcga gccacgctta aagggttata gactcgcgca aatcgttgaa atatttttga
     7861 ctgatcttca actgcaatac ctactccggt atcctctacc cggacatgag cttgattctc
     7921 tgaaaaccgt aggcttactc caatactacc ccctttatgc gtatatttac tagcattatt
     7981 cacaatattt tccaacgcca tatgaataag cttaggatca cagaccactg ctgatcgatg
     8041 agacggcaag attaactcca agcgctggct gcgttccatc gcaactggct gatgcacttc
     8101 aataatatcc tccaatattt cagctatatc ggcgcttacc atttccggca taatgcggcc
     8161 gctatcaatt ctggcaacat ttaacatgtc attaataatt tccagttggc gctcgtttgc
     8221 cttccggatt ttttgcatgt actctttttg gcgctcactc agcttacccg catagccgtc
     8281 gagcagtaaa gaaatgaatg ccttggctcc ggtcgctggt gtacgcaact gatgcgaggc
     8341 cagcgagacg aactcttctt tggcgcgctc aagctcgcgt tcgttggtaa ggtcgcggaa
     8401 tacctcaata gccccgactg ggcggccgtc cagtatgacg ggagatacgg ttaccgctac
     8461 tgggaagacg ctgccatcct tgcgctgata ccagacggtt tcgctaatgc tcctgccgct
     8521 cattaacgcc tgagtagccg gacgattcag tggatcgatg gatttacctt gatcatcaag
     8581 cgctaacacc gtaccgggaa accatttgcc ggtaagtgtt tttacatcgt accctagtgc
     8641 cgcagccgcc gaagagttaa cgctggcaat tacgccatgc tcgtcaatca ccagcagccc
     8701 ctcgccaata ctgcttagta gcgtttgggc ttgtatcttt gccgcttcag ctgccttttt
     8761 ttgttcttcc agctttttaa gtgctagtct ttcctggcta atatcaaagg tgctgaggac
     8821 gagtccggtt atgcgcccat cggtaaaaat cggtccaacg ctgtttttat accattgctg
     8881 gctgttattt atgtaggtcg tatactctag ttcggtggag cgcccggttt tcatcacgcg
     8941 tcgcaagatt ttctgaacgc ggtggcgctc cgatgccttc ataaaatc
//
LOCUS       DASVGB010000040         5766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_761878, whole genome shotgun sequence.
ACCESSION   DASVGB010000040 DASVGB010000000
VERSION     DASVGB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761878"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VF272_01245"
     CDS             <1..724
                     /locus_tag="VF272_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7259538.1"
                     /translation="EDMLASELAAKVPDELRRIKHAQAITDQTFTDILGIIKAGMTEK
                     AVAAEIVYLLLKHGADQIPEHFWPIVASGPNSAIVHAHPTDRKLSDGDVVLLDFGCLV
                     EGYTSDMSRTLVLGKASDKIKKVYAIVLEAQQAALAAAKAGISGMKLDAVARSIIESA
                     GYEIPHGVGHSLGLEIHEEPRINPRNPNPLPENAVMTIEPGIYIPGEFGVRIEDMVRL
                     TKIGSENLTASPKELIELHPAR"
     gene            complement(678..1217)
                     /locus_tag="VF272_01250"
     CDS             complement(678..1217)
                     /locus_tag="VF272_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259539.1"
                     /translation="MILLLLDFDRTVFNLSRFLHDEANASLALDYLFEDAGRLLGALP
                     ENVTPAIISQATIIGEDLQGGVDWQHHKFAFAPALADLPFCVTTMNKGFVLAAGVTIV
                     RDGELAVDAGPIQGTYEEIILIDDNAASFKPLIASNSQIAMYHLARPGEKYSDKPATA
                     SVGRITSLDEAQSILLAKP"
     gene            complement(1214..2380)
                     /gene="yvcK"
                     /locus_tag="VF272_01255"
     CDS             complement(1214..2380)
                     /gene="yvcK"
                     /locus_tag="VF272_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEX7259540.1"
                     /translation="MKGNAADIIKIGNSKGIRIPKALLQQTGLTDRAQLSVQEGQLVV
                     RPEGEPPTSVQPPSSVGVKAVVIGGGTGSFMLLSALRPYVGNLTAIVTMSDDGGSTGI
                     LRDELGVLPPGDVRQCLVALSNTPEYMRELFNYRFEEGTFRGHSFGNLFLTALEKISG
                     GFGEGVKLAEQILQVSGQVIPVTTSDIRLTAVMSDGTQLNHERNIQTHDFSGDRPLLQ
                     LEPQAKVNKEAVRAIMAADMVIVGPGDLYGSIIPVLLPDGMAKALERTKAPKVYVANL
                     VNKPDKTKGMQVHEFLDELSRHLDGKQLFDYVIYNTAMPTPTLAKRYLRNGELPVEFE
                     AATLKQQPYKAIGEPLLAGAAPENKNDVLLEYQRSLIRHDGDRLARALMRIYFS"
     gene            complement(2421..3281)
                     /locus_tag="VF272_01260"
     CDS             complement(2421..3281)
                     /locus_tag="VF272_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7259541.1"
                     /translation="MSKPPITTAIIGDNGFTTRRLPISATIPKGFTPIGNRPVVDFIV
                     DELVKVGVEDIYFVIHPYHQDIYERYFLGNDLLDKHLAATRKDTDLNMLKALRGRAQF
                     HFVTKTERYGTAIPPLLAFEDMGGFRSCIYMASDDFTYRSDGGSDLVDLVELYASREA
                     VGGLLGLHLPDEMLSKFGVMQVESREDVCFLKNLIEKPEHPEKLEGTLLANIGKHIIG
                     TEMEPYFQNLKAHPVTGEYFIVDVYAAVAREHEVVVLPVKGDYYDVGNLDNWMNVNKA
                     LAKIPIDLKQ"
     gene            complement(3309..4721)
                     /locus_tag="VF272_01265"
     CDS             complement(3309..4721)
                     /locus_tag="VF272_01265"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX7259542.1"
                     /translation="MSAHKSSEQSHSNPISLDELVSLAKRRGFIYQGSEIYGGLAGTW
                     DYGPYGVLLKKNIKDLWWQHFVTERDDMYGLDTAILMNAKVWEASGHTGAGFADALVE
                     DKETHMRYRADHLLEDNGVDNAAELSIPEMDAKIKELGLKSPDGNVLTPVRVFNMMFQ
                     TQIGAIEDTSAKVYLRPETAQGMFVNFKNVLDSLHPKLPFGLAQIGKNFRNEITPKDF
                     IFRVRELEIMEFEYFIREGEWEQRFGELHDSMRAWFRRIGLSDDTMKEVEVPEADRAH
                     YSKKTTDFYFKFPHKFDEIGGLAYRTDFDLSNHTKHSGIDLSYMDPQTNERFIPHVIE
                     PTFGLDRHVLAVMAAAYTEEEVNGETRTVLKLKPELAPVKVAVSPLLRNKPELVKIAQ
                     AVYKTLKKDFGSVLYDDNGNIGKRYRRQDEIGTPLCVVVDFDSLDDKQVTIRHRDSLA
                     QVRVPIAELPTHITQQLTDF"
     gene            complement(4832..5551)
                     /gene="recO"
                     /locus_tag="VF272_01270"
     CDS             complement(4832..5551)
                     /gene="recO"
                     /locus_tag="VF272_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7259543.1"
                     /translation="MAIYSTDAIILGRHNFGEADRVMVLLTPGRGVIRGVAKGVRRIK
                     SRLAGHLELFCHSELMLAEGRNMDTITGARLVHHPEMSNDYERMRLAYLFAEMVNRLS
                     INEEPHPGMFELVQEAYLGLREPDPVLELWFKLRLLDRLGYRPELNACIICDSADAGR
                     TYLFNAELGGIVDQGCSTPGNHALSHDQIKLWRLMMGHSLGDIRRVEQAPELALAALP
                     ICNAFYDYTFGKRFRSAEVLR"
BASE COUNT         1471 a         1540 c         1348 g         1407 t
ORIGIN      
        1 tgaagacatg cttgcatctg agctagcggc taaagtccca gacgagctta gacggattaa
       61 gcacgctcag gcaattaccg accagacatt caccgatatc ctcggaatta tcaaggccgg
      121 tatgacggaa aaagcggtgg cagctgaaat cgtgtacctc ctgctaaagc acggcgccga
      181 tcagataccc gagcatttct ggccgattgt agcatccggc ccgaatagcg caattgttca
      241 tgcccatcca accgaccgta aactatcaga cggcgacgtt gtactactcg attttggctg
      301 tttggttgaa ggatacacct ccgatatgag ccggacgctg gtacttggca aagccagcga
      361 taaaatcaag aaagtttatg cgatagtcct ggaagcgcag caagcggcac tggcggcagc
      421 taaagccggg atcagcggca tgaagctgga tgccgtggca cgttcgatta tcgaatcagc
      481 aggatatgaa atcccccacg gcgttggcca ttcgcttggg ctagaaatcc atgaggaacc
      541 acgaattaac ccgcgcaacc ccaacccgct tcctgaaaat gccgttatga cgattgagcc
      601 cggtatctat attcctgggg aatttggtgt tcggattgaa gacatggttc gcttaaccaa
      661 aataggctca gaaaacctta cggcttcgcc aaaagaattg attgagcttc atccagcgag
      721 gtaatccgcc caacactggc cgtcgccggt ttatcactat acttctcccc tggccgcgcc
      781 aaatgataca tagcaatctg cgagtttgag gcaatcaacg gcttaaatga tgctgcattg
      841 tcgtctatca atataatttc ttcataggtc ccttgaatcg gcccggcatc gaccgccagc
      901 tccccatctc ttacaattgt aacgccagcg gctaagacaa agcctttatt catagtagta
      961 acgcaaaatg gcaggtcggc aagcgccggc gcaaaggcga acttatggtg ctgccaatct
     1021 actcctccct gtaagtcttc accgataatg gtggcctggc tgatgattgc cggagtgaca
     1081 ttttcaggta atgcgccaag cagccggccg gcatcttcaa aaagataatc cagtgccaag
     1141 gaagcgttcg cctcgtcatg caaaaaccgg ctaaggttga aaacggtccg gtcaaagtcg
     1201 agcagtaaca gaatcatgaa aagtatatcc tcatgagagc acgggccagg cgatcgccgt
     1261 cgtggcgaat taaactgcgc tggtactcca agagtacgtc attcttattt tccggtgcgg
     1321 ctcccgctaa gagcggttcg ccaatagcct tgtagggctg ctgcttgagc gtggcagcct
     1381 caaactccac cggcagctca ccgtttcgaa ggtaacgttt ggctaaggtt ggcgttggca
     1441 tagcggtatt atagatgacg tagtcgaaca actgtttgcc atccaaatgg cggctgagtt
     1501 catccaaaaa ctcatgaacc tgcatccctt ttgtcttatc cggtttattc accaaattag
     1561 caacgtacac cttgggcgcc ttggttcgct caagtgcttt cgccattccg tctggcaaaa
     1621 gcaccggaat aattgacccg taaaggtctc ccgggcccac aataaccata tcagccgcca
     1681 taattgcacg aaccgcctcc ttattaacct tagcctgggg ctcaagctga agcagcggac
     1741 gatcgccgga aaaatcgtgg gtttgaatat tacgctcatg attcagctga gtgccatcag
     1801 acattaccgc cgttaaacga atatcgctgg tggttactgg aataacctga ccgctgactt
     1861 gcaagatttg ctcggccagc ttcacgcctt cgccgaatcc tccggaaatt ttttcaagtg
     1921 cggttaaaaa taagttgcca aacgagtgcc cgcggaacgt tccttcttca aaccgataat
     1981 tgaacagttc gcgcatatac tccggcgtgt tcgatagggc caccaaacac tggcgaacat
     2041 cccctggagg taagactcct aattcatcgc gcagtatgcc ggttgagccg ccatcatcgc
     2101 tcatggtcac aattgccgtc aaatttccaa catacggccg taacgccgaa agcagcataa
     2161 agcttcccgt gcccccgccg attaccaccg ctttcacgcc aacagaagat ggcggctgga
     2221 ctgatgtggg cggctccccc tccggccgca caaccagctg accttcctgt accgataact
     2281 gagcccgatc agttaatccg gtttgctgta acagagcttt aggaatccta atgccctttg
     2341 agttaccaat cttgataatg tcggccgcgt tacccttcat atattctgcc tgcagcttta
     2401 gtaattacat tgtaattaca ttattgtttt aaatcaatag ggattttggc tagtgcttta
     2461 ttcacattca tccaattatc aagattgccc acatcgtaat aatctccctt taccggcagt
     2521 actaccacct catgctcgcg agccaccgca gcataaacat caacaataaa atattctccg
     2581 gtaactgggt gagcttttaa attctggaaa tatggctcca tctccgtgcc aataatatgc
     2641 ttgccaatgt ttgcgagcag cgtcccttcc agtttttccg ggtgttcagg cttttcaata
     2701 agattcttaa ggaagcacac atcctccctg ctctctacct gcatcacccc gaactttgaa
     2761 agcatctcat ccggcagatg caatccgagc aagcctccaa cggcttcgcg ggatgcataa
     2821 agctcaacca ggtcgaccaa atcactacca ccgtcagaac gataggtaaa atcatctgat
     2881 gccatataaa tgcatgaacg gaagccgccc atatcttcaa atgccagcag tggcgggata
     2941 gcagtgccgt aacgttcggt tttagtgaca aagtggaact gtgcccgacc acgcaaagct
     3001 tttagcatgt tcaggtcggt gtccttgcgg gtagcagcca ggtgcttgtc taataaatca
     3061 ttccctaaga aataacgttc ataaatatcc tgatgatacg gatggatcac gaaataaata
     3121 tcttcaaccc caaccttcac cagctcatcc acaataaaat ctaccaccgg ccggttgccg
     3181 ataggtgtaa aaccctttgg tatggttgcc gatattggca gccttctagt agtaaagccg
     3241 ttatcgccaa taatggctgt cgtaatgggt ggtttggaca tagtacccat aatagcaaat
     3301 aaccggactt aaaagtcggt taattgttgc gtaatgtgcg taggcagctc ggcaataggc
     3361 acccgaacct gagccaagct gtcacgatgc cggatggtaa cctgtttatc atcgagcgaa
     3421 tcgaagtcta cgaccacgca taatggtgtg ccaatttcat cctggcggcg atagcgcttg
     3481 ccaatattgc cgttgtcatc atataacacg ctgccaaaat cttttttcaa ggttttatac
     3541 acggcttggg cgatcttgac caactccggc ttattgcgca gcagcggcga cacggctact
     3601 tttaccggcg ccaactccgg cttcaacttt agaaccgtcc gggtttcgcc gtttacttct
     3661 tcttccgtgt aggccgctgc catcaccgcc agcacatgcc ggtcgagccc aaacgttggt
     3721 tcaataacat gcgggataaa acgctcattg gtttgcggat ccatgtagga aaggtcgatg
     3781 ccgctgtgct tggtatgatt gcttaggtcg aaatcggtac gataagccaa tccgcctatt
     3841 tcatcaaact tatgaggaaa cttaaagtag aaatcggtgg ttttcttgga ataatgagct
     3901 cgatcagcct caggaacctc aacctctttc atggtgtcat cggataggcc gatgcggcgg
     3961 aaccaggcac gcattgagtc atgcagctca ccaaaccgtt gctcccactc cccttcacga
     4021 atgaagtatt cgaactccat aatttcaagt tcccgaactc gaaatataaa gtcttttggc
     4081 gtaatctcgt tacggaagtt tttaccaatc tgggccaagc caaacggcag ctttggatgg
     4141 agactatcca gcacattttt aaagtttacg aacatgcctt gggccgtttc cggacgcagg
     4201 taaacctttg ccgacgtatc ttcaatggca ccgatctggg tttgaaacat catgttaaag
     4261 acacgcaccg gcgtgagcac atttccgtcg ggactcttaa gaccaagttc tttgattttg
     4321 gcatccatct ctgggattga aagctcggcc gcattatcga cgccgttatc ttcaagtaaa
     4381 tgatcggcgc gataccgcat atgggtttct ttatcctcta ccagcgcgtc ggcaaacccg
     4441 gcgccggtgt ggccgctagc ctcccacacc ttggcattca ttagaatcgc tgtatcgaga
     4501 ccatacatgt cgtcacgctc agtgacaaaa tgctgccacc agagatcttt aatattcttt
     4561 ttgagcagca caccatatgg gccatagtcc caagtaccag ccaaaccgcc ataaatttcc
     4621 gagccttggt agataaaccc gcggcgcttg gcaagcgata cgagttcgtc gaggctgatt
     4681 gggttggagt gggattgttc agatgatttg tgggcggaca taatgaagct tattatacgg
     4741 caaacctacc ttctgcgcga cacaccagag ttccggtgga gtcacgggga attgaccccg
     4801 cacgcggggc gggctttcag aataagctca attagcgtaa gacttcagcc gaacgaaaac
     4861 gtttgccaaa ggtgtagtca taaaaagcgt tgcagatggg cagagcggca agggcaagct
     4921 ccggggcctg ctccacccga cggatatcac ccaggctgtg acccatcatg agccgccaca
     4981 acttaatttg gtcgtggctt agcgcatggt tacccggtgt actgcagccc tgatcaacta
     5041 tgccgcccag ctcggcatta aataagtagg tgcggccagc atcggcagaa tcgcaaatga
     5101 tgcaggcatt tagctccggc cgatagccaa gccggtcaag caagcgcagt ttaaaccaca
     5161 gctccagtac tgggtccggc tctctaagcc ctaggtaggc ttcctgtacc agctcaaaca
     5221 ttcccggatg cggctcttca ttaatactca aacggttgac catttcagca aacaaatatg
     5281 ctagacgcat ccgctcataa tcgttgctca tctcaggatg atgcaccaat cgagcaccgg
     5341 taatggtgtc catgttgcgg ccttcggcta gcattagctc gctatggcaa aacagttcca
     5401 aatgaccagc caaccggctt ttaatgcgcc gcacgccttt tgctacgcct cggatcaccc
     5461 cccggccagg cgtgagcagc accattaccc tatcggcttc gccaaagtta tggcggccta
     5521 gaataattgc gtcagtagaa tagatggcca tagctctagt gtactactac tgcttggctt
     5581 cgctgtagct atctcttaat gcacatttgt aacaaagtgt tacaaatgtg cattaaggaa
     5641 aaacggcaat tttaaaagca gccaccccgc ttttcggcgc gactgctcca tcttgtacgt
     5701 gaagtgcgga aagcttaggc tagcgccttc atcacctttt cgaccacttc atcgggagta
     5761 tggtgg
//
LOCUS       DASVGB010000041         7552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_295126, whole genome shotgun sequence.
ACCESSION   DASVGB010000041 DASVGB010000000
VERSION     DASVGB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295126"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF272_01275"
     CDS             <1..181
                     /locus_tag="VF272_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259544.1"
                     /translation="GTSMARGWHGDSAGHARAGQLGGRKSAETRKQTRTTNFGEQLQA
                     PNNQKTQRKSNDAKG"
     gene            complement(188..457)
                     /locus_tag="VF272_01280"
     CDS             complement(188..457)
                     /locus_tag="VF272_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259545.1"
                     /translation="MATKQSPGSKKKHDDNFYETMPGGLYIDSNELFPGRFADEADYT
                     DHAGQDEDRNQVRNRMMRQSLILVSLLVAQIVLFMLLITYTVNLG"
     gene            complement(621..1271)
                     /locus_tag="VF272_01285"
     CDS             complement(621..1271)
                     /locus_tag="VF272_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259546.1"
                     /translation="MKVVAKRVVVHYTQSAWTIDIRVDGLILGRRPWTRFDERPFSSK
                     QAVRDRENELTTALLRHLLDVRTDWIELWPDRLKWGMLQPFGSQLYLNPLLTRLRAAP
                     NSQIHQTARLPAVRATVLELPSSAELGIDYWRRCFLRPQDEPLQLVALLDGDPEPVVL
                     EFQVTSLDNNQGREQPYVSVLVRQRNTTRRWQPVRFRVPALNPDKWCVFDYELEDF"
     gene            complement(1451..1849)
                     /locus_tag="VF272_01290"
     CDS             complement(1451..1849)
                     /locus_tag="VF272_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259547.1"
                     /translation="MIAADRLANLADLRDIVNRDVELARDIQNERGPNRFRGPLLCVQ
                     VLGDGSVKFIRRRTAMAAMNSNRWTRVHGKDEITVSPDSFFILVDGCICCMTPLGNVT
                     IFRDGDKLDFAAVAPAAADIGAGVPAAIAA"
     gene            complement(2042..2428)
                     /locus_tag="VF272_01295"
     CDS             complement(2042..2428)
                     /locus_tag="VF272_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259548.1"
                     /translation="MDVQDLLQRAGIADGRMYVRAGGRAYSAPITHITNKGERVFFQC
                     SPVAICSFGQLLELPGPMHVIVILPLYAEIRSSASGYRVDHANGQVRIEPNPEKVKAH
                     RRQQLMPWGALSAVSVPDFIPPDLLH"
     gene            complement(2503..3939)
                     /locus_tag="VF272_01300"
     CDS             complement(2503..3939)
                     /locus_tag="VF272_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7259549.1"
                     /translation="MKKSGSSYLYEYKRLFANPGSPRKPSRDQENTDVQKHILDVLLL
                     GAFILGTLAFIASGVHQLFMKGSSESGSPFASLFFCMIVFGLYQVARTFSYRLSAYLL
                     ITLFILAGTYTVYRWSIDIPIGLLIFGLVISMSGILISSVFALVVTAIIAIILTTIGL
                     LQTNEIIKPDVSWMLHPVEFGDAITFIALFVIISLISYLSTREINKSLQRAHKSEQAL
                     LKERSSLERKVVERTAQLEALQKARLIQLQRFADFGRISVGLVHDIANPLTVVSLRLK
                     QLDSSKNSKLISEAMKSMKVLERYIDATRRQLQDKDEYEHESFEISDEISQVVDVISY
                     KANMESVKIKSQIRPNVCIFGDPCKFSQIIANLVVNAIDAYKVIPLKQNRLVLISLKD
                     GECGTIITVQDWGKGIEGNEIEKIFEPFYGVENSGKRGLGIGLSIVRRIVEEDFGGSV
                     TVTSSRVDGTQFVVRLPAHKSETVSIQG"
     gene            complement(3936..4613)
                     /locus_tag="VF272_01305"
     CDS             complement(3936..4613)
                     /locus_tag="VF272_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7259550.1"
                     /translation="MKLLIIEDSLMVLAALRQSLHPHYVIDVATSGEEGLDVAVSNTY
                     DIILLDLGLPDIPGDQVCTLIRKASVTTPILVLTASLKIRDKVLLLDSGADDYLTKPF
                     SLEELKARIRVLIRRDTPSKTSLIKLGDIVLDPITRRVYKKSFLIELRRKEFDLLEYF
                     MINAGKVLTRPMILDHVWEMNEGIWTNAVDVHIKYLRDKIDRPFGTKMIETVHGVGYR
                     IEGVPKK"
     gene            complement(4690..5589)
                     /locus_tag="VF272_01310"
     CDS             complement(4690..5589)
                     /locus_tag="VF272_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7259551.1"
                     /translation="MPVTIGTMHIPQCQPVSFLSTDQLQLKGLLFGNAEAVSAYIVIH
                     GLTGSAFSQHETVVPLANERTQVLTFSNRGHDKIAYFKRTDPNSEKGYVSEKIGEAHE
                     VFTECIHDIQGAITFLEERGVQAIHLVGHSTGCHKAMYYLSQKPSKSVKGAVLLCPLS
                     DYAYFRYSLDEAIMSNAITIAQKMVGAGKPHELLPLDIWPGLEDAQRFLSLATPDSEE
                     EIFTYAQPDKEPETLQKVTVSLNIILAEQDEARDRPITEIGEWFKTVLPEEQIKVSII
                     PGATHNLIGKGEEVREGIKLYIP"
     gene            complement(5640..5918)
                     /locus_tag="VF272_01315"
     CDS             complement(5640..5918)
                     /locus_tag="VF272_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259552.1"
                     /translation="MRPILADLVLVDCRWYRPLAATVAGLAAAAEAGGTVMVTTIKAE
                     HVMMTASAITAVSSRVKRFFSVNIRGIPPTPRWLPYVKTRRRVTKGDA"
     gene            complement(6019..6186)
                     /locus_tag="VF272_01320"
     CDS             complement(6019..6186)
                     /locus_tag="VF272_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259553.1"
                     /translation="MQGLGAGSRSAAGDSAGRREVGRHAASDGGDANALGESAEGTLL
                     HPEVDRRDVAA"
     gene            complement(6320..6880)
                     /locus_tag="VF272_01325"
     CDS             complement(6320..6880)
                     /locus_tag="VF272_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259554.1"
                     /translation="MRFSCKQKVSVAGASTVALIWLWPMAATKFTELKNLTNQKSLEV
                     DSRKAILRLVGEEPRQMPWPCVGDLTVNELVRIDPRTFHALRASTPGASVVAARWAEN
                     LNPERLAEAVVYMSASQLAGFVILFSAHPGAFESGRGLPVLLVLSMHLARNLDLLEGV
                     RHELGKAFNPAATLYLGWMRGEEIAS"
     gene            complement(7054..7359)
                     /locus_tag="VF272_01330"
     CDS             complement(7054..7359)
                     /locus_tag="VF272_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259555.1"
                     /translation="MKTLKIRRSKQSKARTALGKAASLLEAIVPPKKATTMTKVKAKA
                     TSKKTIVAVGAGTSLAALAVHKINRSDPLGDTLGDTTNTNGHMAAATSSSATPLAVR"
BASE COUNT         1809 a         2016 c         1866 g         1861 t
ORIGIN      
        1 gggtaccagt atggcacgag gatggcacgg agactcagcc ggacacgcac gagccggaca
       61 gctgggcgga cgaaaatcag ctgaaacaag aaaacaaacg cgcacaacaa actttggcga
      121 gcaattacaa gcgcccaaca atcagaagac gcagcgaaaa tcgaatgatg ctaagggtta
      181 gttacgacta tcccaggttc acggtatagg ttataaggag cataaataaa actatttgag
      241 ccacaagcaa gcttaccaaa attaggctct gccgcatcat ccggttacgt acttggttcc
      301 gatcttcatc ctgtccagcg tggtcggtat agtcggcttc atcggcgaat ctgccgggaa
      361 acagctcgtt ggagtcaatg tacaatcccc ccggcatggt ctcataaaag ttatcgtcat
      421 gctttttctt cgaacccggt gattgtttgg ttgccatagt attgctcctc ctcttctctt
      481 gttctactct acctcggcag ctagcattac tgcattggcg gattatggaa ataatccagt
      541 ccttccctcg gactttttcg gcatttagcc ataagcctta acgtgcagac gccccgcaag
      601 cggggtgcct gcggggcgga tcagaagtcc tcgagctcgt agtcgaaaac gcaccacttg
      661 tccgggttca aggccggtac ccggaatcgc accggctgcc acctccgagt ggtgttgcgc
      721 tgacgaacca gcacgctcac gtaaggctgt tcgcggcctt ggttgttatc cagggaggtc
      781 acctggaatt ccagaacaac cggctcggga tctccgtcca gtagtgccac cagttgcagc
      841 ggctcgtcct gaggtctcag aaaacagcgc cgccagtagt caatgccgag ttccgcagac
      901 gatggcagct cgagcacggt ggctcgaacg gcgggaagcc gtgcggtttg gtgaatctgg
      961 ctgttcgggg cagcccgcaa tcgggtaagc aaggggttga gatacaactg gctgccaaac
     1021 ggctgcagca ttccccactt cagccggtcc ggccacagct cgatccaatc ggtccgcaca
     1081 tccagcaaat gccggagcag tgcggtggtg agctcattct cacggtctcg tacggcctgc
     1141 ttcgacgaaa agggccgctc gtcaaagcgg gtccatggcc tgcggccgag aatcaagccg
     1201 tcaacccgaa tgtcgatcgt ccatgccgac tgggtgtagt gcactacgac ccgctttgcc
     1261 actactttca tacatccccc tagaggtcgg aattgccacg tcatgtcgaa gacacgttac
     1321 ggtattgtgc ctcatgcagc ttatatattc aagcagaata agtccaagaa gggtcccgcc
     1381 gcaagcgacg ggtccgcttc ttaatggcgg ccgtggcccg cagcgggcgg gatggagggc
     1441 gacgatgagc tcaggcggcg attgccgcag gtacgcctgc gccgatgtca gcggccgccg
     1501 gcgccacagc ggcgaagtcg agcttgtcgc catcgcgaaa gatggtgaca ttgccgagcg
     1561 gtgtcataca gcagatgcag ccatcgacca ggatgaagaa cgagtcgggt gagacagtga
     1621 tctcgtcttt gccgtgcacc cgcgtccacc tgttcgaatt catggccgcc atggcggtgc
     1681 ggcggcggat gaatttcacc gagccgtcac cgagcacctg gacgcagagg agcgggccgc
     1741 gaaagcggtt gggaccccgc tcattctgga tgtcgcgggc caactctacg tctcgattga
     1801 cgatgtcgcg gagatccgcc aggttcgcca acctgtctgc agcaatcatt gtgaatcctt
     1861 tcttcgggat actcttcaac aggagtgctg aagagcactt atagtagcat aagcgatata
     1921 tgccaagcaa ttttttataa gtccgagtga gcccgccgca agcggcgagc ccacttgtga
     1981 aagtgaaact gccgcccgcg gagggggttt ggtcgtgatg atgatgtacg gaaggtgcct
     2041 gctagtgcag gagatccggg ggaatgaagt cgggcaccga aaccgccgag agcgctcccc
     2101 atggcatgag ctgctggcgc cggtgcgcct tgaccttctc cgggttgggc tcgatccgaa
     2161 cctgaccgtt agcgtgatca acccgatagc ccgacgcgga gctgcggatc tccgcataaa
     2221 gcgggaggat gacgatcaca tgcatcggcc caggcagctc aaggagttga ccgaagctgc
     2281 atatggccac cggtgagcac tggaagaaaa ccctttcacc tttgttggtg atgtgggtga
     2341 tgggagccga gtatgcccgg ccgccggccc gcacgtacat gcggccatcg gctatgccgg
     2401 cgcgctgcag caaatcttgc acatccatgt gatgtccctt cgttcaaccc gcgtggacag
     2461 atcagcgaac caagcataat gatgacacaa gtaacgatga tgtcaacctt gaatgctcac
     2521 cgtctcgctc ttgtgcgcag gtagtcgtac aacaaattgc gttccgtcaa ctcttgagct
     2581 agtaacggta actgagccac caaagtcttc ttccactatc cgcctaacta ttgaaagccc
     2641 aataccaagt ccacgcttgc cagaattttc cacaccatag aatggttcaa aaattttctc
     2701 aatctcattt ccctcgatgc ccttacccca gtcttgcact gtaataatag ttccgcactc
     2761 tccgtccttt aaggatatta aaaccagcct attttgcttt aatggaataa ccttatatgc
     2821 atcaattgca ttaactacaa gattggcaat aatttgacta aatttacacg ggtcgccgaa
     2881 tatgcaaacg tttggccgaa tttgactttt gatttttaca ctctccatat tggctttata
     2941 tgaaattaca tccactactt gagaaatttc atcactaatt tcaaagcttt catgctcata
     3001 ttcatcttta tcttgaagtt ggcgcctagt ggcatcaatg tacctctcca gaaccttcat
     3061 agacttcatc gcttcactaa ttaatttgga attctttgag ctatcaagtt gcttgagcct
     3121 aagtgagacc actgtaagcg gatttgcaat atcatgcacc agccccacgc ttatgcggcc
     3181 aaaatcagca aatcgctgca actgaatcaa tcgtgctttt tgcaaagcct ccagttgagc
     3241 tgtccgctca acaacttttc tctctaagct acttctttct tttagcaacg cctgctccga
     3301 tttatgtgct ctttgcaatg atttattgat ttctctggtt gagaggtatg atatcaaact
     3361 aataatcaca aaaagagcta taaatgtaat tgcatcgcca aattctactg ggtgaagcat
     3421 ccagcttaca tctggcttaa taatttcatt ggtttgaagt agtccaatgg tagtcaaaat
     3481 aatagcaatt atcgctgtca caactagcgc aaagaccgaa ctaatcagga taccggacat
     3541 cgatataact aggccaaaaa ttagtaatcc tatagggata tcaatactcc atcggtaaac
     3601 agtgtatgtt cccgctagta tgaataatgt aattaataaa tatgcagata gcctataaga
     3661 aaatgtgcgt gcaacttgat ataaaccaaa aacaatcatg caaaaaaata gcgatgcaaa
     3721 cggagagcca ctctcggaac tacctttcat gaatagctga tggacgccag atgcaataaa
     3781 tgcgagagtc cccaaaataa atgcccctag aagaagaaca tccagtatat gtttctggac
     3841 atcagtattc tcttgatccc tcgatggctt gcgaggactt cctgggttgg caaataatcg
     3901 tttatattcg tataaataac tacttccact ctttttcatt tttttggcac tccctcgatt
     3961 ctgtacccca ccccatgtac tgtttctatc atctttgttc caaagggtct gtcaatttta
     4021 tcccgcaaat atttaatatg cacatcaaca gcatttgtcc atataccttc attcatctcc
     4081 catacatggt cgagaatcat tggccgtgtt aataccttgc cagcatttat catgaaatac
     4141 tccagtaaat caaactcctt gcggcgtagt tcaatcaaaa atgatttttt atagactctg
     4201 cgagtgattg ggtcgaggac tatatctccc aattttatta aactggtttt gcttggagtg
     4261 tcccttctta ttaaaacgcg tatacgagct ttaagctcct caagactaaa aggtttagtt
     4321 aagtaatcat cagctcccga atcaagtagt agtaccttgt ccctgatttt taagcttgcc
     4381 gttagaacta atatgggtgt ggtaactgaa gccttcctta ttaatgtgca aacttgatca
     4441 cctggaatat caggtagccc aaggtcaagg agaataatgt catatgtgtt tgagacggct
     4501 acgtctagcc cctcttctcc tgacgtagct acgtcaatta cataatgtgg atgcaagctc
     4561 tggcgcaatg cagccagaac cataaggcta tcttctataa ttaataattt catttttaat
     4621 ccagcgcttt ttattaaaca atacttagtt tgatgcaggt atattaaata aatataaaaa
     4681 ttcttaattt catggtatat ataattttat cccctccctc acctcctccc cttttccgat
     4741 cagattatgc gtcgcgccgg ggataatact cactttaatc tgttcctcag gaagcaccgt
     4801 tttaaaccac tccccaatct cggtaatagg ccgatcacgg gcttcgtctt gttccgccag
     4861 gataatattc agcgataccg taaccttttg caatgtttca ggctctttgt ctggctgggc
     4921 gtacgtaaaa atttcctcct cgctatccgg cgtggctaag ctcaagaaac gctgggcatc
     4981 ttccaggccg ggccatatat caagcggcaa cagctcatgt ggcttccctg ctcctaccat
     5041 tttttgggca atggtaatgg cattcgacat tatggcttca tccagactat acctaaaata
     5101 ggcatagtcg cttaaagggc agagcagcac tgcgcctttt acgcttttac tgggcttttg
     5161 actcaggtag tacattgctt tatgacaacc ggtggaatgg ccaaccaaat gaatggcttg
     5221 cacgccgcgc tcttccaaga aggtaatcgc tccttgaata tcatggatgc actcggtaaa
     5281 tacttcgtgg gcttcaccaa tcttttccga aacgtagcct ttttcgctat tcggatcagt
     5341 ccgcttaaaa taggcgattt tgtcgtggcc gcgattacta aaagttaaaa cctgggtgcg
     5401 ctcgttcgcg agcgggacca ccgtttcatg ctgggaaaag gcgctgccag taagaccgtg
     5461 gatcacgata taggcagata cggcttcggc attgccaaac agcagcccct ttagctgaag
     5521 ctgatcggtg ctcagaaatg aaaccggttg gcactgcgga atatgcatag tgccaattgt
     5581 aacaggcata cctatatgct cttgtaaata ttacgaatat atgctattat agtaggtcgt
     5641 tacgcatcgc ctttcgtaac tctccgtctc gttttgacat aaggcaacca tctcggagta
     5701 gggggaattc ctctgatgtt gacgctgaag aaccgtttca ccctggagct gactgccgta
     5761 atcgcgctgg cggtcatcat gacgtgctcg gcctttatgg tcgtaaccat caccgttccg
     5821 ccggcctcag cggctgctgc cagtccggcg acggttgcgg ccagtggtct gtaccagcga
     5881 cagtccacca gcaccaagtc cgccaagatc ggccgcatgc tcctcagagg cgagcgggtc
     5941 accatgacct gctacgtcat cggcgagttc gtccggaaga accggacgta tagctacccg
     6001 atgtgggctc agctcacctc aggcggctac gtcgcgacga tctacctccg gatgaagaag
     6061 ggtgccttcg gcactctctc ccagtgcgtt ggcgtccccg ccgtcactgg cggcgtggcg
     6121 gccaacctcg cgcctcccgg cactgtcgcc ggcggcgctt cggctccccg ctcctagccc
     6181 ctgcacacct cccgcctgcc ttttcggttt ggcgggaggt tcctgccggg gcccaacttg
     6241 tcttaataaa aatgtaatta ttgcgaagtg gcagcacccg gaagaaataa atcctcccgg
     6301 gtgcgtcgtc ttggtgaggc tagctcgcga tttcctctcc ccgcatccag ccgagataga
     6361 gggtggccgc ggggttgaag gccttgccga gttcgtggcg gactccctcg agcaggtcga
     6421 ggttcctcgc caagtgcatc gagagcacga gaagcacggg cagaccgcgt cccgactcga
     6481 aggcgccggg atgagccgag aacaggatga cgaagccggc cagctgcgag gcgctcatgt
     6541 acacgacggc ttctgccagg cgctcaggat tcaggttttc cgcccaacgg gccgcaacca
     6601 ctgatgcacc cggggtagat gcccgcagtg cgtggaaggt gcggggatcg atccggacca
     6661 gctcattgac cgtaagatcg ccaacgcacg gccatggcat ttgccgcggc tcttcaccaa
     6721 ccaagcgcag tattgctttt cgactgtcga cctcgagcga cttctggttg gtcaggttct
     6781 tgagctccgt gaatttggtg gccgccatcg gccagagcca gatcagggca actgtgctgg
     6841 caccggcgac cgaaaccttc tgcttgcaag agaagcgcat ggttctccaa ttgtcagggg
     6901 cgtgcgccca tatacaagac cgagtaatta tactataaat ataagaaaat acaacccccg
     6961 atttaaaggg caggtacccg ggaagaggct atcctcccgg gtacgcaatt gcgtgtccta
     7021 gccaataaag cccagtgcgt tctggttgtg cggctatcgc acggccaacg gggtcgccga
     7081 cgacgatgtg gccgcggcca tatggccatt ggtgttcgtc gtgtcgccga gcgtgtcgcc
     7141 cagcgggtcg ctgcggttga tcttgtgcac agccagggca gccaggctgg taccggcacc
     7201 gaccgccacg atcgtcttct tggaagtggc tttcgccttg accttcgtca tcgtcgttgc
     7261 cttcttgggc ggaacgattg cctcgaggag cgaagctgcc ttgccgaggg cagtacgcgc
     7321 cttgctctgc ttgctgcggc gaatcttcag cgtcttcatg cgacctccag cttcagggcc
     7381 taagcccagt tgtataggac tgggccagac tactacagct ggtacggcaa ttcaaccaat
     7441 taaattacaa attattttga taaaacgcta ccgcacgttg caaaaataac cggcggtttt
     7501 gaataaactg atgatcatct cctgggtaca cgtggtattc aaccggcttc cc
//
LOCUS       DASVGB010000042         4209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_755747, whole genome shotgun sequence.
ACCESSION   DASVGB010000042 DASVGB010000000
VERSION     DASVGB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755747"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VF272_01335"
     CDS             <1..1057
                     /locus_tag="VF272_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7259556.1"
                     /translation="TMSVATLGVSSITAIGYQGTPGNYSNVQSNGNCTYSSVRAESPL
                     DPNRMNDRCLNHEYGHAWSYYYGVEFQQDSKFTSYLKARGLDGDSRVNSSYQWYTAEM
                     IAEDYRQLLGSPNTQDGQINWEIPFAKDVPGLKDYLLTAFRIRLVQPVDNQAPTAPAN
                     LQRANYSTNTVSLTWNASTDNTGVAGYELYRNGVQVANPIEPSFLDIGLTPGTEYSYV
                     AIAYDAAGNHSAPSAALVTSTASNATPPPPTSNVVISNLLISPNPVVLSGKTNPIIKY
                     SLSQSSIASVSITNSSDGLVRKLTTNLQQSGNLQLSWDRKDANGRKVRAGTYVVKVEA
                     TGSSGNPVTQMLTFRAK"
     gene            1069..1482
                     /locus_tag="VF272_01340"
     CDS             1069..1482
                     /locus_tag="VF272_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259557.1"
                     /translation="MRKSIVISSTLVAIFAFYFYGLPYLLAINEPKLPDVHQKEKVVS
                     EEKAATNLPKLLVQVTKSGFQPSSIKAKSGQRIEFYNASGANIKLVSDSANKPAFGGK
                     VIPNNTYYTVSFNEKTMVNFYNEINVSQKGSITVE"
     gene            1862..1972
                     /locus_tag="VF272_01345"
     CDS             1862..1972
                     /locus_tag="VF272_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7259558.1"
                     /translation="MSFGAGSYVLRYMVEKNDAVIVIRVWHSREYRSASA"
     gene            2048..2137
                     /locus_tag="VF272_01350"
     tRNA            2048..2137
                     /locus_tag="VF272_01350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2082..2084,aa:Ser,seq:cga)
     gene            complement(2188..3087)
                     /locus_tag="VF272_01355"
     CDS             complement(2188..3087)
                     /locus_tag="VF272_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259559.1"
                     /translation="MSKSRQASKFAVLAVGTYAAAMVVGVMATPLSSTYIAQRAADYA
                     MTARNSSEPSQQVGNYRLALALDPGNSTYRSELADTYIKQNNPSGAINALGNTVGERV
                     RKAGYQLSLGRYEQAKRSIASLKSPEAAIIRSKIALEQGGDQKAYQAVIAARNDAERL
                     QLGLSYASANMNDDITKIFPLMGEGESKRRLQRAQSGGVALAQELYKERLYRSAERIL
                     AKAGESGAKYLLLAHVQMNLPENDDQVKLEAAKNSLKQGIKRDPANLTLRLLLVEIYH
                     RLEDENGATRENQVIQRLKTGKL"
     gene            complement(3062..>4209)
                     /locus_tag="VF272_01360"
     CDS             complement(3062..>4209)
                     /locus_tag="VF272_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7259560.1"
                     /translation="TGDFDRFYPTSVMETGRDILFLWVTRMVMLGLYATGEVPFKNVY
                     LHGMVNDAHGKKMSKSKGNVVNPLEWTERYGTDALRLALTIGITPGNDGALSEKKVEG
                     YRNFNNKLWNVARFILDKAGDDYSPSSPKVKSLADAWIVTKFSRENAAITDAIENYRF
                     SEAGERVYSLLWNDLADWYIEASKTEANLDALVYGLETILKLTHPFAPFVTEAIWQNL
                     PWQSRNLISEPWPEAGERHDSKTIQFEAIMALVSQIRTISSDLKLSKPTLLSKDDLIN
                     ENRALIKRLARLGEIQPIEASRGLRVPGSVAAWLDIPDADIQNYQKQLASRRSETATY
                     LKKLEAQLQNQAYLKGAPASLVKETKDRAEAAKLLLLQLDEQIKASQ"
BASE COUNT         1070 a         1045 c          967 g         1127 t
ORIGIN      
        1 gactatgagt gtggcaacac ttggagtttc gagtatcacg gcgattggct accaaggcac
       61 gccaggaaat tactctaatg ttcaatcaaa tggcaactgc acctactcat ctgtgcgggc
      121 agagagtcct ttagatccaa acagaatgaa cgaccgatgc ttaaatcatg agtacggaca
      181 tgcttggagc tattactatg gtgtagaatt tcagcaagat tcaaaattca cgagttattt
      241 aaaagctcgt ggactcgatg gcgactcacg ggttaattca tcttaccagt ggtacactgc
      301 tgaaatgatt gccgaagatt atcggcagct ccttgggtcg cccaatacgc aagatgggca
      361 aatcaactgg gaaattcctt ttgcaaaaga cgttccgggg cttaaagatt atctgctaac
      421 agcctttaga atcagacttg ttcagccagt cgacaaccaa gcgccaaccg ctcctgctaa
      481 tttacagcgt gctaattaca gtactaatac cgtatcactt acttggaatg cctcaactga
      541 taataccggt gttgccggct atgagcttta tcgaaatggg gttcaggttg caaatcctat
      601 tgaaccgagc tttcttgata tcggcctaac acccggaaca gagtatagct atgtggctat
      661 tgcctatgat gcagccggca atcactcagc tccctcggcc gcccttgtga cctctacagc
      721 ttcaaatgct acaccaccac cccctacgtc aaacgtggta atttcaaacc tcctgattag
      781 cccgaaccca gtagtgctat ccggtaaaac caacccaatc attaagtaca gtttgtcaca
      841 atcgtccata gctagcgtgt caattaccaa ttctagcgac ggactcgtgc gcaagttgac
      901 aaccaatctt caacaatccg gtaatcttca gctttcatgg gaccgcaaag atgcaaatgg
      961 ccgtaaagtt agagctggca cctacgtggt caaggtggaa gcaaccggtt ccagcgggaa
     1021 ccctgttaca caaatgttaa cctttagagc taaataatgc ttacgccaat gcgcaaatca
     1081 atcgttattt cctctacatt agtggccata tttgcctttt acttctacgg tctgccctat
     1141 ctattggcga ttaatgagcc taagctgccc gatgtacatc aaaaggaaaa agttgtctcg
     1201 gaggaaaaag cggccacgaa ccttccaaaa ttgctagttc aggtaaccaa aagcggtttt
     1261 cagccaagct ctatcaaagc taaaagcggg cagcgcattg agttttacaa cgcttcagga
     1321 gcgaatatta aacttgtcag tgatagtgcc aataagccag cgtttggcgg gaaagttatt
     1381 cctaacaaca cctattacac agtcagtttc aacgagaaga cgatggttaa tttttataat
     1441 gaaataaacg ttagccaaaa aggctctatt acagttgagt aggtgatccg ctatttgccg
     1501 tcaccgcacc gctatacaat aataaaatgg ttttaacctc agccagtgtg ttgcaccatt
     1561 ggacttatgc ttaagttgtc gttatcctgg ttgcataaag ttgcaacaag tgttactatg
     1621 actcgtataa ggaagaaaat gcacgtgact tcaacacagg gcaaagcggc ttcattgcat
     1681 cagtaaaatg gctttccgaa gctctagatg acgttgagcg gcttcatcag ttcttgcatg
     1741 gcaaaagccc tgaggcagcc gcgcgcgcgg caaatatcat cctagaaggg gctctattgc
     1801 ttaaaggcac ccctcgcctt ggcaggccta tgacagatac aactggcagg gggagctgtt
     1861 tatatctttc ggagcgggat cctatgtact gagatacatg gtagaaaaga atgatgcggt
     1921 tattgttata cgtgtctggc atagccgcga atataggtcg gcttcggcct aaccagttgc
     1981 ctcaagtgat ccgctatacg ataataaatg agttctatct tctgctagtg taccgcaaga
     2041 agtacctgga gaggtggccg agtggttgaa ggcgccgctc tcgaaaagcg gtatatcggc
     2101 aacggtatcg tgggttcgaa tcccaccctc tccgccaaag tgaagcatta ttcgtctgcg
     2161 gcgcattaaa ttttcatagt gtaagagcta taatttcccc gttttcagtc gttgtataac
     2221 ctgattttca cgagtagctc cgttctcatc ttctaaacga tggtagatct ccacgagcaa
     2281 caaccgcagc gtaaggttcg ccggatcacg ctttattccc tgttttaagc tgtttttggc
     2341 agcctccagc ttgacttgat catcgttttc tggcaggttc atttgcacat gagccaataa
     2401 caagtatttt gcacccgact cccccgcttt agcgagtatc cgttcggcac tgcgatacaa
     2461 tcgttcctta tagagttctt gcgccaatgc cactcccccg ctctgggccc gttggagccg
     2521 gcgcttcgac tccccttccc ccattaaagg gaaaatctta gtgatatcgt cattcatgtt
     2581 agccgaagca tagctaagac ccagctgtaa ccgctcagcg tcgtttctgg ctgcaattac
     2641 ggcttggtac gccttttggt ctcccccttg ctccaaagcg atcttagagc gtattatggc
     2701 tgcctcaggg cttttcagtg aagcaatgct ccgcttggct tgctcgtagc ggccgaggct
     2761 taactgatac cccgccttgc gcacccgctc ccccacggta tttcccaggg cgttgattgc
     2821 cccgctcgga ttgttttgct tgatgtaggt gtctgctagc tcgctccggt aggtgctatt
     2881 acctgggtca agcgccaatg ctaaacggta attaccaacc tgctgactcg gctcggaact
     2941 gttgcgcgct gtcatagcgt aatcagccgc gcgctgagct atgtacgtgc tgctcagggg
     3001 agtcgccatc accccgacaa ccatagccgc ggcataggta ccaactgcta gtactgcaaa
     3061 cttactggct tgccttgatt tgctcatcta actgcaatag tagcagtttt gcggcctcgg
     3121 cgcggtcttt cgtttctttc actaacgatg ctggtgcacc cttcagatag gcctggtttt
     3181 gcagttgggc ttccagcttt ttcaaataag tggcggtttc agatcggcgg ctcgccaatt
     3241 gcttttgata gttttgaata tcagcatcgg gaatatcgag ccaggccgcc actgagccgg
     3301 gaacgcgcag tcccctacta gcttctatgg gttgaatttc tccaaggcga gccaatcgct
     3361 taataagcgc tctattctca ttaataagat cgtctttgct gagcaaggta ggcttagaca
     3421 gttttaaatc gcttgaaata gtacgaattt ggcttactag agccataata gcttcaaact
     3481 gaatagtttt actgtcatgc cgctcccccg cttcgggcca cggttcggaa attagatttc
     3541 ggctttgcca aggtaaattc tgccagattg cttcggttac gaacggagca aatggatggg
     3601 ttagcttgag gatggtttcc agcccgtata ccagagcatc aagatttgcc tcggtttttg
     3661 aagcttcaat ataccagtcg gcaagatcat tccacaaaag agagtagacg cgctccccgg
     3721 cttcgctaaa gcgatagttt tctatagcat cggtaatagc cgcgttttca cgtgaaaact
     3781 tagttacaat ccaggcatct gctaggctct taaccttagg ggaagaaggg gagtagtcat
     3841 ctcctgcttt atcgagaatg aaacgcgcta cattccagag cttattatta aaattacgat
     3901 aaccttcaac ctttttctcg cttaatgccc catcattacc aggtgtaatg ccaatggtga
     3961 gagccaggcg cagggcatcg gtgccgtaac gttcggtcca ctccaggggg ttaaccacat
     4021 tacctttaga cttgctcatt tttttaccgt gggcatcatt aaccatgccg tgaagatata
     4081 catttttaaa tggcacctcg ccggtggcat aaagtccaag catcaccatg cgggtaaccc
     4141 aaaggaacag aatatcgcgg ccggtttcca ttaccgaggt agggtagaaa cgatcgaaat
     4201 caccggttg
//
LOCUS       DASVGB010000043         6073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_918358, whole genome shotgun sequence.
ACCESSION   DASVGB010000043 DASVGB010000000
VERSION     DASVGB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918358"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF272_01365"
     CDS             complement(<1..331)
                     /locus_tag="VF272_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7259561.1"
                     /translation="MLYKLRWIIASLITLIAVTIFAFLIWFLWAARPADPDAKQPISY
                     RLENGTSVDEIARRLEEEGIIKNAAAFNLFVTFRGLRSGLKAGTYELNPNETMNDIAQ
                     QLAEGRIA"
     gene            complement(321..722)
                     /gene="ruvX"
                     /locus_tag="VF272_01370"
     CDS             complement(321..722)
                     /gene="ruvX"
                     /locus_tag="VF272_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7259562.1"
                     /translation="MTDSILAIDYGKKRVGLALAVQGVPQRLQTLPNDELLVGRLADI
                     VQNHQIGLVVLGLPRTSNGDDTAWTAEVRQFAKNLGGEISAPVTLRDEFATTDEARAR
                     LVSQKLEPGSQKALLDQEAAVVLLEDYLNAL"
     gene            complement(712..2394)
                     /locus_tag="VF272_01375"
     CDS             complement(712..2394)
                     /locus_tag="VF272_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259563.1"
                     /translation="MAQDPKQASNGVIYLEVDEDITAAVDKLSKSESEKVQLVTTKRS
                     TLLQSVINLKLLQKAAKGSNKELIIVTTDRVATSLAGRLGIPVASQVGETAKVPSAMA
                     AVAAADDEIDGGMVGEPTMNAATGMTTPLTAMAAEPPLPPSTPPSAPAMPSNTQVAAP
                     KPKGQHVPSISGMQKRVLWGVAGVVGLGMIFGIFYYLASAKVTLFANASQVNTKFAFT
                     ADPAAKRSDIGNNLLSAQRLSLSKAVTASVEATGTKDLGSKARGSVIVSNSYDSNDHP
                     LVSGTRFVASNGLVFRSTEDAVVPGGSLKSGKVVPGQVSVAVQADQNGDQYNLGPGKY
                     TIPGLPADQQSGISGQGEQMSGGTTKTAKVVTQPDIAKAQAAALESDRPASAKELANK
                     ASDSQTVIDASLQQNVTSSDSNPAVNSEAQTATLTLQVTYSQLAVEKSDLSKLARTEE
                     SKQLGGQSQIYDDGSDKLEIALVAKPQSADSAQKFNAAATAFAGTKIDTAALAKELKG
                     KKYGDALDIAQRVPGVDRAEISLKPGWFTRLPGISSHINITIKVANTSSSDR"
     gene            complement(2394..3656)
                     /locus_tag="VF272_01380"
     CDS             complement(2394..3656)
                     /locus_tag="VF272_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEX7259564.1"
                     /translation="MAFKLVDKIRKKSDSEQYIVALDIGTEFVKALIGRVNGNQIEII
                     GAGRQHQRLTDMASGAVTDIAGVVDNCDAALKQAEEMAGVVAKDVVLGIAGELVKGTS
                     TTIKYKRTDAQTHIEMSELRKILERVQERAFEKARETLAWETGQQEIDVKMVNTAIVD
                     VQIDGYRVTNPIGFQGRDVSIQLFNAFAPMVHIGALQQVGNNLDLNIINIAAEPFAVA
                     KSVGGEDTSDFSAIFIDIGGGTTDIAVVDNGGVEGTKMFGIGGRSFTKRIAQMMGISF
                     EKAEELKLEYSAGKLAHVKDHEAVKEAVKADINVWLSGVELSLAEFTKIDHLPAKILL
                     CGGGTGLPEIMQALKSDWYKELPFARKPIISHIQPSMVSRVVDKTGQLTSYADITPMG
                     LANLGLDVLNTGSISESVLQKLSKTLQN"
     gene            3808..4215
                     /locus_tag="VF272_01385"
     CDS             3808..4215
                     /locus_tag="VF272_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259565.1"
                     /translation="MKTSTAKSNPAMPPSVMFVVSHDFANGVKSTQIQFSLPDLGEAE
                     MKQLLADINGMWNVQDVRPGFKTTRGVTLIVTLKCTQVTRLAIYVIAHAIRRAADAEA
                     IYVWGMLQRQYLPNKQIDILLRKRKPGPYITIG"
     gene            complement(4286..4441)
                     /locus_tag="VF272_01390"
     CDS             complement(4286..4441)
                     /locus_tag="VF272_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259566.1"
                     /translation="MLNASTAESRGSIIFELLGGILLIGAVSLGVYELQHPELTQQQQ
                     IQAALEH"
     gene            complement(4623..>6073)
                     /locus_tag="VF272_01395"
     CDS             complement(4623..>6073)
                     /locus_tag="VF272_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX7259567.1"
                     /translation="AKVIELGSQKDGDKKGMQGGRIFYYDDGENWWSRGGGLDATPIG
                     DPAGPDSEVFYDFGEEQHDANFGQPHPASDSGRFMEIGNQVFMQYLRTKDGFEPLEKN
                     NVDFGGGLERIAAAAINSPDVFKISLLWPIVETLEQLSGKKYDDNTEAMRVIADHLRG
                     AVFLAVDGVVPSNKEQGYVARRLMRRAIMKAHDLGITKDFVKEVVPVIADLYHKDYPE
                     VTERREPVIDALVREEKIFRQTLRNGIRELEKIAKVSVGDKGKVVITGEHIFTMYDTH
                     GFPVELTLEEVTHHKDWVLADTWQQHFEAEMVNQRERSQTASKGVFKGGLADQSDDTK
                     KHHTATHLMYEALRRVLGEHVTQRGSNVTAERLRFDFNHDTKMTDEERAKVEQIVNEQ
                     IDNDLEVSFTEYPTDEAFKLGAKGAFGDKYGEKVKVYAMGKPGKKPYSIEICGGPHVV
                     HTGVLGEGGKRFKIQKEESSSAGVRRIKAALV"
BASE COUNT         1374 a         1715 c         1378 g         1606 t
ORIGIN      
        1 cggcgatgcg tccttccgct agttgttggg cgatgtcatt cattgtttca ttgggattta
       61 attcatacgt cccggccttc agccccgacc tcagcccccg aaaggtaacg aagaggttaa
      121 aagctgcagc attcttaata atgccctctt cttccagacg cctggcaatt tcatcgacgc
      181 tggtaccgtt ttctaggcga taactaattg gttgtttggc gtcaggatcg gccggccgcg
      241 cagcccagag aaaccaaatc aggaacgcaa aaatggttac agcaatcaga gtaatcagcg
      301 aagcaatgat ccagcgcaac ttataaagca ttgaggtagt cctccaacaa caccaccgct
      361 gcctcttggt cgagcagtgc tttctgcgag ccaggctcaa gcttttgcga gaccagtcga
      421 gccctggctt catcggtagt ggcaaactca tcccgtaacg ttaccggcgc gctaatctct
      481 ccgcctaaat ttttcgcaaa ctggcgtacc tctgcggtcc aggcggtatc gtcgccgttg
      541 ctagtccgtg gcaatccgag caccaccaag ccgatttgat ggttttgcac aatatcggcc
      601 agtcggccga ccagtagttc atcgttaggc agtgtttgaa gcctctgtgg cacaccttgc
      661 accgccaatg ccagtcctac tcgctttttc ccgtaatcaa tggccagaat actatcggtc
      721 actggaactg gtattcgcta cttttatggt gatgttaata tggctgctaa ttcccggcag
      781 ccgggtgaac catcccggct taagactaat ttccgcccgg tctacaccag gaacccgttg
      841 ggcaatgtcg agtgcgtccc catacttctt gcctttaagt tccttggcta gggctgcggt
      901 atcaatctta gtgccggcaa atgcggtagc tgccgcgtta aatttctgcg cagaatctgc
      961 cgactggggc ttggcaacca atgcaatctc caacttgtcg ctaccatcgt catagatttg
     1021 actctgtccg cctagctgtt tactttcctc ggtgcgggcc agcttggaca aatctgattt
     1081 ttcaaccgcc agctgcgaat aggtaacctg caaggttagg gtggcggttt gagcctcgct
     1141 gttcacggcc ggatttgagt cgctgctcgt aacgttttgc tgcaatgagg catcaataac
     1201 ggtttggctg tcgctggcct tattggcaag ctccttagct gaggctggcc tatcgctttc
     1261 caaggctgca gcttgagctt tggcaatgtc aggttgggtt accactttgg cggttttagt
     1321 agtacctcct gacatttgct ctccttggcc ggaaatcccg ctctgctgat cagctggcaa
     1381 gccggggatt gtgtacttgc caggtcccaa gttgtactga tcgccattct ggtcggcttg
     1441 aaccgccacg cttacctgac ccggcactac tttaccactt ttgagcgatc cccctggcac
     1501 aaccgcatct tcagtagacc gaaacaccaa gccgtttgaa gccacaaacc gtgttccgct
     1561 caccagagga tgatcattcg aatcataact attagaaacg atgacactgc cgcgcgcctt
     1621 ggagcccaag tctttagtac cggtagcttc aacgctggct gtaaccgcct tacttaagct
     1681 aaggcgctgt gcagaaagta gattattgcc aatatctgaa cgcttggcgg ccggatcggc
     1741 agtaaacgca aacttggtat tcacttgtga tgcatttgca aataacgtaa ccttggcgct
     1801 ggctaaataa tagaaaatcc caaagatcat gcctagtccc accacacccg ccacgcccca
     1861 aagcacgcgc ttctgcatcc cgctaatact gggaacgtgc tgaccttttg gtttcggagc
     1921 tgccacttga gtattggacg gcattgcagg ggcggatggc ggcgttgacg gaggcaaagg
     1981 tggctcagct gccattgctg ttaagggagt agtcatgccg gttgctgcat tcatggttgg
     2041 ctcgccaacc attccgccgt cgatttcatc atcggcagca gcgacggcag ccatagctga
     2101 aggaactttg gcagtttccc ctacttgaga cgccacgggt atacccaacc ggccagcaag
     2161 gcttgtagca actcggtcgg tagtaacgat gattaactct ttattgctgc ccttggcggc
     2221 tttctgcagc aactttaaat taataaccga ctgcaataag gttgaacgct tggtggttac
     2281 cagctgtact ttttctgact cagatttact cagcttatca accgctgcgg taatatcttc
     2341 atcaacctct aggtagataa cgccgtttga tgcttgtttg ggatcttgcg ccattagttc
     2401 tgcaacgtct tacttaattt ttgcagtacg ctctcgctaa tactaccggt gttcagcaca
     2461 tccagcccaa ggttcgccaa gcccattggc gtaatatcgg cataactggt aagctgcccg
     2521 gttttatcaa caactcggga aaccatagaa ggctggatat ggctgataat tggcttgcgc
     2581 gcaaacggta gttccttata ccagtcgctt ttcaaagcct gcataatctc cggcaatccg
     2641 gtgccgccgc cgcataacag tatcttagcc ggcaaatggt caattttggt aaactccgcc
     2701 agactcagct ccacgcccga aagccagaca ttaatatcgg ccttaaccgc ctctttgaca
     2761 gcctcgtgat ccttcacatg agccagctta cctgccgaat attccagttt tagctcctcg
     2821 gccttttcga agctaatccc catcatttgg gcaatccgct tagtgaagga ccgcccgccg
     2881 atgccaaaca ttttggtacc ctcaaccccg ccgttatcca ctaccgcaat atcggtagtg
     2941 ccgccgccaa tatcaataaa aatggcgctg aagtcgctgg tatcttcgcc gcccacgctt
     3001 tttgctaccg cgaacggttc agcggcaatg ttgataatat ttaaatccaa attatttcct
     3061 acctgttgca gcgctcctat atgtaccatc ggagcaaaag cattaaacag ctggatcgaa
     3121 acgtcgcgtc cctgaaaacc tatcggatta gttacacggt agccgtcaat ctgcacatcg
     3181 acgatcgccg tattcaccat ttttacatca atttcctgct gacccgtttc ccaggccaag
     3241 gtttcgcggg ctttttcaaa tgcccgctcc tgaacccgct ctaaaatctt gcgcagttca
     3301 ctcatttcga tatgggtttg agcgtcggtc cgcttgtact taatggtggt cgaagtgcct
     3361 ttcaccagtt caccggcaat acccagcacg acatctttgg ctaccacgcc agccatctct
     3421 tcggcctgct ttagagcggc atcgcagtta tcaaccacgc cggcgatgtc ggttaccgca
     3481 ccggaggcca tatcggtcaa tcgctgatgc tggcggccgg caccaataat ttcaatttga
     3541 ttaccattaa cccgaccaat cagagctttt acaaactcgg ttccaatatc cagcgccacg
     3601 atgtactgct cgctgtcgct ttttttccga attttatcaa caagtttgaa tgccatacaa
     3661 aagtataatc ctacaggatt attgtaacgc gtccaaccgg cttttaccat aagcagctta
     3721 cttgagccca aaggttgacg aaatacttac ttagtggttt aatatagcca cgctgaattt
     3781 cgcccgcaac gcttccggga ggaagacatg aagaccagca ccgccaagtc gaaccccgcg
     3841 atgcctccct cggtgatgtt cgtggtttcc cacgacttcg ccaacggcgt caaatccacg
     3901 cagatccagt tctcccttcc tgatctaggg gaagccgaga tgaaacagct tctcgcggat
     3961 atcaacggga tgtggaatgt gcaggatgtg cgacccggat tcaagaccac acggggagtc
     4021 acgctgatcg tcaccctcaa gtgcacccag gttacacgcc tcgcgatcta tgtgatcgcg
     4081 catgcaatcc gaagggcggc cgatgccgag gcgatctacg tctggggcat gctccagcgg
     4141 cagtatctcc ccaacaagca gatcgacatc ctgctgcgca agcgcaagcc gggcccgtac
     4201 atcacgatcg gctgacttag tccgagccaa cccctcaacg ccccgtatca actggaagct
     4261 ccgcttgatg cggggttttc actttttaat gctcaagagc cgcttgaatc tgctgttgtt
     4321 gcgttaactc gggatgctgc aactcatata cccccaggct aaccgctcca atgagtagaa
     4381 ttccccctag taattcaaat atgatcgagc cacgcgattc agcggtgctt gcattgagca
     4441 tttttatctc cccttttttg tttttggtac tatttgtctt tagttaatca aactgcttat
     4501 gcttttgcaa gagttttttt accgaaacat tgcatacgtc tcacgctgca aaatagtttt
     4561 tcggtacact aaatgtatca gatgggtaca cctaatgtcc ctaattcatt ttcagataat
     4621 gcttatacca gtgccgcttt aatccgacgc acgccggcac ttgaggactc ttccttttgg
     4681 attttgaaac gcttacctcc ctcgccgagt acgccggtat gcaccacatg cgggcccccg
     4741 caaatctcaa tactgtaggg ctttttacca ggtttgccca tggcgtaaac cttaactttc
     4801 tcgccatatt tatcgccgaa cgccccttta gcaccaagct taaaggcttc atcggtcggg
     4861 tattcagtga agctaacttc cagatcgttg tcgatttgtt cattgactat ctgctcaact
     4921 ttggccctct cttcgtcggt cattttagtg tcgtgattaa aatcaaatcg cagccgctcg
     4981 gctgtaacat tggaaccgcg ctgagtcacg tgctctccca gcacgcggcg caacgcctca
     5041 tacattaaat gcgtagcggt atggtgcttt ttggtgtcat cgctttgatc ggctagtccg
     5101 cccttaaaaa cgcccttgct ggcagtttgg gaacgctcgc gctgatttac catttctgcc
     5161 tcaaaatgct gctgccaggt atcggccaaa acccagtctt tatggtgcgt aacctcttct
     5221 aaggttagtt caacgggaaa gccgtgcgtg tcgtacattg taaatatatg ctcgccggta
     5281 ataactacct tccccttatc gccaacactt accttggcaa ttttctcgag ttctcgtatg
     5341 ccattacgta gtgtctgccg gaagatcttt tcctcccgca ccagcgcatc gattaccggc
     5401 tcgcggcgtt cggtaacctc tgggtagtcc ttgtggtaga ggtcggcgat taccggcaca
     5461 acttctttta caaaatcttt cgtaatgcca agatcgtgcg ctttcatgat tgcccggcgc
     5521 atcaaccggc gtgcaacata tccctgctct ttattgctcg gcacaactcc atcaaccgct
     5581 aagaaaacag cgccccgcaa atggtcggca attacccgca ttgcttcagt attgtcgtca
     5641 tacttcttgc cggaaagctg ctccagggtt tcaacaatcg gccataagag gctgatttta
     5701 aacacatccg gactgttaat agcagcggcc gcaatccgct ccaatccgcc accaaaatca
     5761 acattatttt tttctagcgg ttcaaagccg tccttggttc gtaaatattg catgaatacc
     5821 tggttgccaa tttccataaa gcggccggaa tcgctagccg gatgcggttg gccaaaattc
     5881 gcatcgtgct gttcttcacc aaaatcataa aacacctcgc tgtcagggcc agccgggtcg
     5941 ccaatgggcg tagcatccag accaccgccc ctgctccacc aattttcgcc gtcatcataa
     6001 taaaagatgc ggccgccctg catgcccttt ttgtctccgt ccttctgact tccaagttca
     6061 atgaccttag cat
//
LOCUS       DASVGB010000044         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_899642, whole genome shotgun sequence.
ACCESSION   DASVGB010000044 DASVGB010000000
VERSION     DASVGB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899642"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1250)
                     /locus_tag="VF272_01400"
     CDS             complement(9..1250)
                     /locus_tag="VF272_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259568.1"
                     /translation="MSRLATFLHAKEPLLDTALHQLEVKTGRKGVDVALTAEIATKAA
                     AFTKQLGLAPDCTGPELYAALTGLTKAHDAHLASTLGCNDPTNVEAIIPLVIRRIDQI
                     HMPRSCFALKYERAGEMLQQHPPQALMQRLGYTDCAAMVAGEDLAELFIAIRFTEGED
                     WLNEFNGLYKDLEATDFEERPIRLVRFDARKWGDVAAHFIVKKKHLNTHSKEMGVIAI
                     LPPIAPHMAAISLKVLTVTLHYYNEVRLYSAYFKLLRHKANFGEILSETLIADTPKVS
                     ITANHHVHWRVIQRYYGKIGESDKHPEVFQPHLQPEDLHWRAAEKMLYQVDPEMAVWR
                     DMDYVGVLKDRDIITFNLMDIVLSFANEVPYEERYLYHFSEALWNEVFARYMGQKTLE
                     KELLIKLDNAMVAPEELRVSR"
     gene            complement(1247..>2164)
                     /locus_tag="VF272_01405"
     CDS             complement(1247..>2164)
                     /locus_tag="VF272_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7259569.1"
                     /translation="TGKALHQKRDVRTHAYVEQFPLGAKLELDHVFFDVGGGASNAAV
                     TFARQGFNSAFAGRVGRDSAGADVLRVLKREHVDTTFVRIDERHGTGYSIILLAPNGE
                     RTVLVYRGASHNLKAEDFQGGKALDAHWMYISSLAGNMELLKKLLKWAHRQAIRVAFN
                     PGSGELAQPRKLRTFLNAVDILIANRDEMELLFGGETAVQTMLNAMPYCPYVVLTDGP
                     NGSYASDGSAIYYAGQYQKVKVVDRLGAGDAFCSGFTAGIARGWAIEEALTLASANST
                     SVVASIGAKSGIMHGTRGMKRMKIKRVSI"
BASE COUNT          537 a          590 c          486 g          551 t
ORIGIN      
        1 aatttttttc atcgagatac gcgaagctct tccggtgcta ccattgcgtt gtcaagcttt
       61 attaacagtt ctttttcaag cgttttttgt cccatgtagc gggcaaatac ctcattccag
      121 agcgcttcgc tgaaatggta aagatagcgt tcttcatacg gaacttcatt ggcgaagctg
      181 agcacgatgt ccataaggtt gaaggtgata atatcgcgat cttttaaaac gccaacgtag
      241 tccatgtccc gccacactgc catttcagga tcaacctgat acagcatttt ttctgctgcc
      301 cgccagtgta aatcctcggg ctgaagatgt ggctgaaaga cttcaggatg cttgtcgctc
      361 tctcctattt ttccgtaata ccgttggatc acccgccaat gaacatgatg gttggcagta
      421 atcgaaactt tcggcgtatc agcaattaag gtttcactta gaatctctcc aaagttagct
      481 ttatgccgca gcaacttgaa gtaggcgctg tatagcctga cttcattgta ataatggagc
      541 gtcaccgtta aaaccttgag tgaaatcgca gccatgtgcg gcgcaatcgg cggcaaaatt
      601 gcaattaccc ccatctcctt agagtgagtg ttaagatgtt ttttctttac gataaagtgg
      661 gctgctacat ctccccactt acgggcatca aaacggacga ggcggatagg acgttcttcg
      721 aaatccgtag cttccagatc cttgtataat ccattgaact cgttcagcca atcttcgcct
      781 tcggtaaaac gaatggctat aaagagctcg gccaagtctt cgccggccac catggcagcg
      841 caatcggtat agccaagccg ctgcattaaa gcctggggcg gatgttgctg gagcatttcg
      901 ccggctcgct catatttcag cgcgaagcag ctcctcggca tatggatttg atcgatccgc
      961 ctgataacca gcggaattat cgcctcaaca ttcgtaggat cattacatcc cagtgtcgag
     1021 gctaaatgtg catcatgtgc tttggtcaag ccagttaagg cagcatagag ttccggtcca
     1081 gtgcagtcgg gagccagtcc cagctgcttg gtgaatgccg cggccttagt ggcgatttca
     1141 gcggtcagcg caacatcaac tcctttacgg ccggttttaa cttccagctg atggagtgct
     1201 gtatctaata aaggctcctt ggcatgcaaa aatgtggcta aacggctcat atactcaccc
     1261 tctttatctt catacgcttc atcccccgag tgccatgcat aatgccgctc ttagccccaa
     1321 tgcttgccac aactgatgtt gagttggcgc ttgccaacgt aagcgcttct tcgatggccc
     1381 atccgcgtgc aatgccggcg gtaaaaccgg aacaaaaagc atctccggca cccagccggt
     1441 caaccacctt aaccttctga tactgcccgg cgtaataaat ggcgctgccg tcagaggcat
     1501 aacttccatt tggtccatcc gttagcacca catacgggca gtaaggcata gcattaagca
     1561 ttgtttgaac tgccgtctca ccaccgaata gcagctccat ttcatcacgg ttggcgatta
     1621 aaatatcaac ggcattaaga aatgtacgca gtttgcgcgg ctgagccaat tctccactcc
     1681 ccggattaaa agccacccgg attgcctggc gatgagccca cttcaacagc tttttaagca
     1741 gttccatatt gccagccagc gatgaaatat acatccaatg cgcgtcaagc gctttccctc
     1801 cttgaaaatc ctcggctttc aagttatgtg aagctccccg atacaccaaa accgttcgct
     1861 caccgttagg tgccagcaaa attattgagt agccggttcc gtggcgttca tcaatccgta
     1921 caaacgtagt atcgacgtgt tcgcgcttta aaacccgtaa aacatcagca cccgccgaat
     1981 cacgtccaac cctgccggca aacgcactat taaacccctg gcgagcaaac gttacagcgg
     2041 cattactggc cccgccgccg acatcaaaaa aaacatgatc gagctccagt tttgcaccca
     2101 gggggaactg ctcaacatag gcatgggtcc gcacatcacg cttttggtgc agtgcctttc
     2161 cggt
//
LOCUS       DASVGB010000045         5194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_891891, whole genome shotgun sequence.
ACCESSION   DASVGB010000045 DASVGB010000000
VERSION     DASVGB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891891"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1687)
                     /gene="lysS"
                     /locus_tag="VF272_01410"
     CDS             complement(143..1687)
                     /gene="lysS"
                     /locus_tag="VF272_01410"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7259570.1"
                     /translation="MATEPTSNYWLDIAAEDINKRYPEGEIVVSSGISPSASYHIGHF
                     REVMTNEAMAWALRQRGRTVRHIHVVDNFDPLRTWYDFLPPAKQDYAGWPVALIPDPE
                     GDCHASYADHFYSLFEISFEPMGLTGATFEIIKSYEDLYKPGHMNRYIEKALEQTPKI
                     KTIFKQLVNRDLSEDWSPVLVLDETGRFVKGDIATWDRDAKTMNGHPYENGLAKLDWR
                     LDWPARWVYLGVNVESFGAQEHGAAGGSYQTGAEFVKQIFGGQPPYGEVRYGHIHRLG
                     ENLKMSSSKGNVITPTDALEIMPAEVFRYFMVRSRPERKLYFDSGLGLFNLIDEFAAA
                     QADPHHEFRDAYEFAVAGKTERVISRVSFKHLVQVYQAAQGDIEGTLQTLERTGYEAA
                     VKVEKAIITEELKFVHNWLKKHAPEAVRFSVQQKLPEVNLNETQTAFLSQLADAVKEY
                     EAELDGKAMHVLIYAAAEKTEIGPKDGFSALYRVILNKDYGPKAGWFLASLDRDWLVK
                     RLKLEE"
     gene            complement(1718..2005)
                     /locus_tag="VF272_01415"
     CDS             complement(1718..2005)
                     /locus_tag="VF272_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX7259571.1"
                     /translation="MKKQITPEGTASASPGSEPKSVRFNVSMQVELLNRLDDAARQEY
                     TTRSDLIRTAVLWYLRPQGPELDRTDPDLIVKTLQQRKLRAGVKKMLKDAE"
     gene            complement(2096..2569)
                     /gene="greA"
                     /locus_tag="VF272_01420"
     CDS             complement(2096..2569)
                     /gene="greA"
                     /locus_tag="VF272_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7259572.1"
                     /translation="MDATAKKDFYLTREGADKLKAELEDLVKIQRPKVAGELKEAKEY
                     GDLSENASWDAAKDHQSFIEGRISELEHILRNIVIIEAPKKKTKISIGSTVHLEIENG
                     KQVYTIVGSTEANPTEGKISNESPIGQALMGKGKGEEIMLNVPSGEMVFKIMHIE"
     gene            complement(2750..4783)
                     /gene="mrdA"
                     /locus_tag="VF272_01425"
     CDS             complement(2750..4783)
                     /gene="mrdA"
                     /locus_tag="VF272_01425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7259573.1"
                     /translation="MSKSLFGYAGETNAQKGRPTIKRDKGGHEQWSDAILPADAAAGV
                     LETPTNKRPLLIVAGLIILGAVALAMQLAHLQLMEGQRNLGLADGNRLREKVTRAPRG
                     IIYDRNKVVLAENLASVDITVTPSLLPRNDAAFRSLLYGKVAGLLGIPPSEVAAKAEG
                     ECQAKEVAAGKKPAIALAACLRDSRARLIVSNVERDRWLAFDQESGTLPGFSLDTNPI
                     RQYNDDTLSAILGYTGRVSQPDLDADPSYKSTDFIGKLGIEKQYETLLRGQNGIEQTE
                     VDATGRPIKLLASRPAKAGSNLTLTIDLELQHKLTDAIKAQMQASGATQAAGIAINPK
                     NGEVLAVVNLPGYDNNLFARGISQNEYQKLVKNGAQPLFNKAIAGAFPVGSIIKPLIS
                     SAALQERAITPSTTVTDTGSIVITNKYDPSIKYTYRSYDPGGHGSVNVAKAIAVSSDV
                     FFYTIGGGHGAIAGLGVTKLANYYNKFGLGQMPAIDLPEKTAGRVPTPEWKQKFSGQP
                     WTVGDTYNISIGQGDLLASPLQMAVAEAAVANGGKVIQPHFFKELAGNNGDVTRTAQV
                     KIQTENFIDSAHLNLVKRAMRDVVRQSYGTACCRIEQSVPVAVAAKTGTAETDPNGKR
                     KPHAWFTAFAPYEDPQIEIVVLVQNAGEGAQYAAPAVRETLEWCFTRPGGCIK"
     gene            complement(4780..>5194)
                     /locus_tag="VF272_01430"
     CDS             complement(4780..>5194)
                     /locus_tag="VF272_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259574.1"
                     /translation="PSVMVIAVVLISLWGTASFSLASAIGGGLLLDMASGSDFGLRMA
                     FFVVISLAIVAGRQLGLQADSWFSALALVALATFLYNAVIVATLQAGVSGIILGRVGA
                     DMLITTTLALVVYGVRLLILANRPVLQKLTRDVWQ"
BASE COUNT         1251 a         1414 c         1217 g         1312 t
ORIGIN      
        1 tgaactggca gccagaaact cggccacgct ttccatagtt ggtgcttggc gatgggcgct
       61 caactgaggc aacagctctg ctgcagcggt ccgcagttcc tcggtagctt ctgttgcaac
      121 ttcaacctct aatgccatat gtttattcct ctagctttaa gcgcttcact aaccaatctc
      181 gatccaggct tgccaaaaac cacccggctt ttggcccgta gtctttattt aagataacac
      241 gatacaaggc tgaaaagccg tccttgggtc cgatttctgt cttctcggct gccgcataaa
      301 tcagcacatg catcgccttg ccgtcaagct cagcttcata ttctttaacg gcgtcagcca
      361 attgagacag aaaggcggtc tgggtttcat tcagattaac ctcgggaagc ttttgctgaa
      421 cgctaaatcg caccgcttcc ggtgcatgct tcttcagcca gttgtgcaca aatttcagct
      481 cctcggtaat gatggctttt tcaaccttaa ccgccgcctc atagccagtg cgttccaggg
      541 tttgaagggt tccttcaata tcaccctgag ccgcctgata cacctgcacc aaatgcttaa
      601 aagaaaccct ggagattacc cgttcggttt taccggctac cgcaaattcg tacgcatcgc
      661 gaaattcgtg atggggatca gcttgggcgg cagcaaactc atcaattagg ttgaatagcc
      721 caagaccgct atcgaaatac agcttgcgct cagggcggct acgcaccata aaatagcgga
      781 acacctcggc gggcataatt tccagcgcat cagtcggagt gatgacgttg ccctttgacg
      841 agctcatttt cagattttcg ccgagccggt gaatatgtcc gtaccgcacc tcgccgtaag
      901 gtggttgtcc gccaaaaatc tgcttcacaa actcagcacc ggtctggtac gaaccgccag
      961 ccgcaccgtg ttcttgagcc ccaaacgatt caacgtttac gccgagatac acccaccgtg
     1021 ccggccaatc cagtcgccag tcgagtttag ccaggccatt ttcataagga tgtccgttca
     1081 tcgttttggc gtctcggtcc caggttgcga tatcgccctt cacgaaccgg ccagtttcat
     1141 caagaaccag caccggcgac cagtcttccg acaggtcgcg attaaccagt tgcttaaata
     1201 tcgttttaat tttcggcgtt tgctccaagg ctttttcaat gtagcgattc atatgaccag
     1261 gcttatacaa atcttcataa cttttaatga tttcaaaggt tgcaccggtt agccccatcg
     1321 gctcgaacga aatttcaaac agactataaa aatgatcggc ataggaggcg tggcagtcgc
     1381 cttccggatc tggaatcaga gcaaccggcc atccggcata atcttgtttg gcaggcggta
     1441 aaaaatcgta ccaggtgcgc aatggatcaa agttgtctac aacatggata tggcgcaccg
     1501 tgcgtccccg ttggcgcaaa gcccaagcca tggcttcatt ggtcataact tcgcgaaagt
     1561 ggccgatgtg ataggacgcc gatggcgata taccacttga taccacaatc tcgccttccg
     1621 ggtatcgttt gttaatatct tctgcggcaa tatcaagcca atagttgctg gtcggttcgg
     1681 tagccataga ggatattgta acaccgagct atgcagacta ctcagcgtct ttaagcatct
     1741 tttttacacc ggcacgaagc ttacgctgct gcagagtttt cactataagg tcggggtccg
     1801 tacgatccaa ctcggggccc tgcggccgca aataccacaa tactgccgtg cggatcagat
     1861 cacttcgggt cgtgtattcc tgccgggcag catcgtccag acgatttaat agctcaacct
     1921 gcatgcttac attgaagcgg accgacttgg gctctgaccc tggcgacgca gaggctgttc
     1981 cctctggagt aatttgtttt ttcatacact aaatgtatca ctcaggtaca tttagtgtat
     2041 ttaaatttta ttaaccattg agcttaggtg ccgcgaactc cataaggcga agcggttatt
     2101 cgatgtgcat aatcttaaat accatttcgc cggacggaac attgagcata atttcctcgc
     2161 ccttgccctt gcccataaga gcttggccaa ttggtgattc gttagaaatc ttgccttcag
     2221 tagggttagc ctcagttgag ccaacgatcg tgtagacctg cttgccattt tcaatttcca
     2281 aatgaacggt agacccgatg ctaattttag ttttcttttt tggtgcttca atgatcacaa
     2341 tattgcgtaa aatgtgctcc agctcagaaa tacggccttc aataaatgat tggtggtctt
     2401 tggctgcatc ccaggatgcg ttctcgctca agtcgccata ttctttagct tctttcagct
     2461 ccccggcaac cttcggacgt tgaattttga caagatcctc cagctcggcc ttcagcttgt
     2521 cggcaccttc tctggtcaga taaaaatctt tttttgcggt tgcatccatg caagccatcc
     2581 tttctctgcc catgttcata ggctgaaaaa aatgctcgag ctatcaactc gagtcaactg
     2641 aatcttaagt agtatttcat gttatgttta taacacgcat tacattgtgc actgggcata
     2701 tacggcggtc aatagttatt actgttaata atttataaca tcgtaccggc tattttatgc
     2761 atcctccggg gcgggtaaaa caccactcca acgtttcgcg aacggcaggc gcggcatact
     2821 gcgcaccctc ccccgcattc tgcaccaaaa ccacgatttc aatctgagga tcctcatacg
     2881 gagcgaaggc ggtaaaccaa gcgtgcggct tacgcttacc gttaggatcg gtttctgcgg
     2941 tgccggtttt agcggccacc gctaccggta ccgactgctc aatcctacag cacgccgtac
     3001 cgtaggattg tctgaccaca tcgcgcattg cccgtttaac aaggtttaaa tgggcactgt
     3061 cgataaagtt ttccgtctgt atcttcactt gagctgtacg cgttacatcg ccattatttc
     3121 ctgccagctc cttaaaaaag tgcggctgga tcaccttgcc gccatttgcc acagccgctt
     3181 cggctacggc catttgaagc ggcgatgcta ataagtctcc ttggccaatc gaaatattat
     3241 aggtatctcc aaccgtccaa ggctgcccgc tgaatttttg cttccattcc ggcgtcggca
     3301 cccggccggc ggttttttcc ggcaagtcga ttgccggcat ctgcccaaga ccgaacttat
     3361 tgtaatagtt tgccagtttg gtaactccca ggccggcaat agcaccgtgc cccccgccta
     3421 tggtgtaaaa gaacacatca gaacttaccg caatcgcctt cgccacattt accgatccat
     3481 ggccgccggg gtcatacgat cggtaggtgt acttaattga aggatcgtat ttattggtaa
     3541 tgacgatcga gccggtatca gtaacggtgg tactgggcgt aattgcccgc tcctgcaaag
     3601 ccgcggagct aattaaaggc ttgataattg agcctactgg aaaggcaccg gcgatggcct
     3661 tattaaagag tggctgagcc ccgtttttaa ccagtttttg gtactcgttt tggctgattc
     3721 cacgagcaaa caaattgttg tcgtagccgg gtaaattaac gacggcaagg acttccccgt
     3781 ttttaggatt aatcgcaatg ccggcagcct gggtggcacc actcgcctgc atttgagcct
     3841 taatggcgtc ggtaagtttg tgttgaagct caaggtcgat cgttaaggtt aggttggaac
     3901 cggctttggc cggacgcgac gccagcaatt taatcggacg gccggtggca tcaacttcgg
     3961 tctgctcgat gccgttctgg cctcgcagca aggtttcata ctgcttctct atccctaact
     4021 tgccaataaa atcggtggat ttatagctgg ggtcggcgtc aagatcgggt tggctgactc
     4081 gcccggtgta gcctaaaatt gccgacaagg tgtcatcgtt gtactgacga atagggttcg
     4141 tatcgagact aaagccaggc aaggtacccg attcttggtc aaaggccagc cagcggtcgc
     4201 gttcgacatt gctcacgatc aggcgagccc gagaatccct gagacaggca gccagagcga
     4261 tcgcaggctt cttaccggca gccacttcct tggcctgaca ctccccttca gccttggcgg
     4321 cgacttcgga cggcggaatg cccagtaacc cagcaacctt gccgtacaga agactccgaa
     4381 aggccgcatc gtttcggggc agtaagctgg gtgtaacggt tatatcaaca cttgccaaat
     4441 tctcagccaa aaccacttta ttgcgatcat aaataatacc gcgcggtgcc cgagtaacct
     4501 tttcacgcaa gcggttacca tcggccagcc ccaaattgcg ctgaccctcc atcaactgta
     4561 aatgagcaag ctgcatagcc agcgcaacgg cacccaaaat aatcagcccg gcaacaatta
     4621 ataatgggcg tttgttggta ggggtctcca atactccagc tgctgcatcg gcaggcagaa
     4681 tcgcatcgga ccactgttca tgtcctcctt tgtcgcgttt tatggttggc cgacccttct
     4741 gcgcattggt ctctccggca tacccaaaca gcgacttgct cattgccaaa catcccgggt
     4801 taatttttgc aaaactggac gattagccaa aatcagtaat cttacaccgt agacaaccaa
     4861 tgccaacgtg gtggtaatga gcatgtcggc gccgactcga ccaaggataa taccggaaac
     4921 tcccgcctgc agggtagcca caatcaccgc gttatacaaa aaagtggcca aggcaactaa
     4981 ggccaaggca gaaaaccagg aatcagcttg aagcccaagc tggcgcccgg caacaatagc
     5041 gagtgaaatt acgacaaaga aagccatgcg cagcccaaaa tcgctgccac tagccatatc
     5101 caaaagcaat ccgccgccga ttgccgaggc caaactaaac gatgcggttc cccacaaact
     5161 tatcagcacg accgcaataa ccattacgct cggc
//
LOCUS       DASVGB010000046         8325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_940877, whole genome shotgun sequence.
ACCESSION   DASVGB010000046 DASVGB010000000
VERSION     DASVGB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940877"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..314
                     /locus_tag="VF272_01435"
     CDS             45..314
                     /locus_tag="VF272_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259575.1"
                     /translation="MIFEHFLIGALLLGIGIATMKFNYQLVGITGYLGFVEKWLGAGS
                     TYGFFKFLSILTCIFGFLYMFGLSDVVLELLLSPLTQFFPKNTGL"
     gene            314..904
                     /locus_tag="VF272_01440"
     CDS             314..904
                     /locus_tag="VF272_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7259576.1"
                     /translation="MQIEQLISYCRLLTQFQQVERQMLVPSGERNENDVEHSYLLAMT
                     GWYIATTAKLDLQHDKVIKYALVHDLVEVYAGDTYFHDPIRTADKVTREEAAFEALRQ
                     NYPDFKELHALIKRYEKQTDAESRFIYALDKLLPVVTIYLDGGRSWHRDKISLELLEF
                     YKSEKVAASAIIADLWSQLAAKLVRQPKLFPPGTKV"
     gene            916..1455
                     /locus_tag="VF272_01445"
     CDS             916..1455
                     /locus_tag="VF272_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259577.1"
                     /translation="MKTPSKIYIVGIAGSGKTTLARRLKRELGYPNLDLDRVRYPDFK
                     TTPDDATLKPSISHLLEQPKWVAEGAYTTWSAPLLEAADSIIWLDTDPAIATYRILKR
                     HIIKLLHGQKHFSNRSTITLAWAALNSRRTGPFLRKTGARQTAIPWHQEIEEGLAPYN
                     EKVLHFDSLNAADKVVFEK"
     gene            complement(1797..3395)
                     /locus_tag="VF272_01450"
     rRNA            complement(1797..3395)
                     /locus_tag="VF272_01450"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(3749..4903)
                     /locus_tag="VF272_01455"
     CDS             complement(3749..4903)
                     /locus_tag="VF272_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259578.1"
                     /translation="MMHHPGRHHYHLIRLSELKRLYWAHTVSMLASSMTGIFIPIYLY
                     RFGYSVPEIMAYYLWFSVFWGAMQYAVLRFANLIGFNRSMGASMIIHGLQILMLATIS
                     VYHWPLWLIALTWALYVAMYWPSFRACFARSLLHKQAGPAVGIATALILCAYGIAPAI
                     GGVIASQLGITVLYMMAMLCFLFAAIPLFGGSEMIRHEPFKLRALQLRRVWRDLVANM
                     GDNIDDSVSTIIWPLFIFLLVPSYMGVGVLSSIAVIASIIIAIYIGRNQARKGTAGYL
                     KRGTLTISLANAVRILVQSSGQIAGINFINGIGHALMSTPYDSRYYRNADREPLLPYV
                     YGMMMAGAAGCFVLFGTLLAVSLVAPITTVLIVGLIMAIPASYIIRLMRV"
     gene            complement(4940..5800)
                     /locus_tag="VF272_01460"
     CDS             complement(4940..5800)
                     /locus_tag="VF272_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEX7259579.1"
                     /translation="MFNRFVVVPLLVWLITQFTKFVVAAFRGKVDFKYLYAAGGMPSV
                     HAAVVTALAVTAGLVEGPRSPIFGVTIVLAAIVMYDAFGVRRAAGDQAVAINTLIDRM
                     KHDDRLPAPAHLKEVLGHKPLEVFIGAVMGLLLGGLFNYDKLGAFNALVIHPVTLPYI
                     YALAAMGVVLLLVGAAARWLLLRSYREIALARQAKNRTSNVLWSFGLACILLAFLQYE
                     KVPAALWVVWPIMLVALLLLGGYVIFRTYYASLPSALASHKVEGQKQRWFEGPNKKRR
                     AKAVKAKKRK"
     gene            complement(5878..8136)
                     /locus_tag="VF272_01465"
     CDS             complement(5878..8136)
                     /locus_tag="VF272_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259580.1"
                     /translation="MTATTFRFQFQRIIAVVLAMTMALAQPYGIAQAADPSDDPPVQT
                     VAPADPSAEVPPADNETADTPLDSTATPTPAPAPPIPPVDPQPITGPSGPTGVQGVTG
                     PTEPTGPGGPTGKVPDYAFNDALQKWEPTISSSFKWSEDAHNWVSPFYRYDPVVGWWH
                     TIPGVTPSDSINALTANSKDPSALMKRILGMNDPEISNTGPNSTNQASVTDSRSVLET
                     LLNNSGIYNDITSGAASGDAGVSNNTDAGDAKTGLATIVTNLLNILNAMWTFGSGGLS
                     YFVENIYGNQTGDIAINPQAVSGGGGQLGQCAGGLSTNTNTGPNSTNIADYDCSANLE
                     VNSQTTGTIVNNVDIDASSGDAAVTGNTKGGSAMSGDVLAELNIINLINTAIGAGQSF
                     FGIINIFGNLDGDILFPSLNINGVVASSQPGTAMLGNSTTGPGSTNTATNSNSNTLDI
                     DNNVDATFNNNIDANAQTGTAAVANNTEAGSATTGNAKTHSSLFNLFNTNIFGDNAVL
                     VIVNNMGRWFGTIMNLGGSGESGGGLLTSNAVVQNANTGPGSVNEASVSNTNNTEIDN
                     KVTGSITNNITINATSGDAMVSGNTKGGNATSGNATIASSIANIFNSALHFGKVFGIL
                     FINVFGTWNGSVGIDTAAGTAPVSPLQLAPLGVGGASFASTLGSGSGSGSSGPIQPAF
                     LASNADNASVATVSASVQSLPNAVQAAIHNPDAAAASTRSTGLLIVLAAFMMLLAGGV
                     LSLDRRLKSSDR"
BASE COUNT         2129 a         2172 c         2002 g         2022 t
ORIGIN      
        1 atctccacca ggaattatca gcatttattt gatacaataa gcttatgatc ttcgaacatt
       61 ttttgatcgg tgccctcctt ttagggattg gcatagctac gatgaaattc aattaccagt
      121 tagtcggcat aaccggttat ctcggatttg ttgaaaaatg gctaggcgcc ggatcgacct
      181 atggattttt caaattttta tctattctta cctgtatttt tggcttcctc tatatgtttg
      241 gtctttccga tgttgtactg gaactacttc tctcaccgct aacccagttt ttccctaaga
      301 acaccggcct gtaatgcaaa ttgagcagct tatttcctat tgccgacttt taacccagtt
      361 ccagcaagta gagcgacaaa tgctagtgcc ctcgggcgag cgcaatgaaa acgatgtcga
      421 acacagctat cttctggcga tgaccgggtg gtatattgcc accaccgcta agctagatct
      481 gcagcatgac aaggtgatta agtatgcgct ggtgcatgat ttggttgagg tatatgccgg
      541 cgacacctat ttccatgacc cgatcaggac ggccgataaa gttacccgcg aggaagcggc
      601 attcgaggca cttcgccaga attatccaga ctttaaagag cttcacgcat taattaagcg
      661 gtatgagaag cagacggatg ctgaaagccg gtttatctat gcgctggaca agctgttgcc
      721 agttgttacg atttatctcg acggcggacg ctcctggcac cgcgacaaga ttagcctcga
      781 gctgcttgaa ttctacaaat ccgaaaaggt tgcagcctcc gcaattatcg ccgatttatg
      841 gtcgcaactg gcggccaagc tggttaggca acctaagctg tttccgccgg gaaccaaggt
      901 ataagcgaca gcagcatgaa aaccccgagc aaaatttata tcgtggggat cgccggaagc
      961 ggcaaaacaa ccttagctcg tcggcttaaa cgtgaactag gctatccgaa tttagacctt
     1021 gatcgagtgc ggtatccaga ttttaaaacc acgccggacg atgccacact gaagccgtca
     1081 atctcccatc tgcttgagca accaaagtgg gttgccgaag gtgcctacac cacctggagc
     1141 gccccgttgc ttgaggcagc cgacagcatt atttggcttg ataccgatcc tgcaatcgct
     1201 acatatcgaa tactaaaacg ccacattatt aagttgctcc acgggcaaaa acatttttcc
     1261 aaccgtagca ctattacgtt agcttgggcg gcactcaatt cccggcgcac cggaccattc
     1321 ttgcgtaaga ccggcgcccg gcaaactgca atcccctggc accaggaaat tgaagaaggt
     1381 ctcgcgcctt ataatgaaaa agtgctgcat tttgattcgc tcaatgccgc tgataaagtg
     1441 gtgtttgaaa aataagcctt ccctactttc acatatcgaa taatcgcttc gctgcatgga
     1501 gcccgcggca agtaagctca ctggttaata aaaattaaac acactaaatg tacctaagtg
     1561 atacatttag tgcatgaaaa aacaaattac cccaggggga acacgctaaa agccgcccat
     1621 tgcgggcggc ttttagtatt gaaatcttgg tagtgcagtg caaaagccat catcttcgac
     1681 ctagaagtac cgcagctgat tcaagcgagc actcttagca agctaagaag ctcaaacatg
     1741 accagtctgg ccataggtct tgcctatgga acgtgttacc acgtcgttct ccctagaaag
     1801 gaggtgatcc atccgcacct tccggtacgg ataccttgtt acgacttcac cccaatcact
     1861 gagtttgcct taggacctag cgtaatgtag gcacgtcggg cacccccaac tttcgtggtg
     1921 tgacgggcgg tgtgtacaag ctccgagaac gtattcaccg tggtgtgctg acccacggtt
     1981 actagcgaat ccaacttcat gcaggcgagt ttcagcctgc aatccgaact gagaccggtt
     2041 ttgatgggat tagctccccc tcgcgggtta gcgacccgtt gtaccggcca ttgtatgacg
     2101 tgtgtagccc agggcataag ggccatgccg acctgacgtc atccccacct tcctcccggt
     2161 tggcccgggc ggtctcgtgt gaaaaataca acacacgaca agggttgcgc tcgttgcggg
     2221 acttaaccca acatctcacg acacgagctg acgacggcca tgcagcacct gtcaccagtc
     2281 cagccgaact gactgcctag tttcttaggc atacgactgg gatgtcaagc cctggtaagg
     2341 ttctacgcgt agcatcgtat taaaccacat catcctccgc ttgtgcggag ccccgtcaat
     2401 tcctttgagt tttaatcttg cgatcgtact ccccaggcgg catacttaac gcgttagctt
     2461 ctcagcacag agggtcgata ctccatacta atagtatgca tcgtttacgg cgtggactac
     2521 cagggtatct aatcctgttc gctccccacg ctttcgtgcc ttagcgtcag aaatatgcca
     2581 gcaagctgcc ttcgccattg gtgttcctac cgatatctac gcatttcact gctacaccgg
     2641 taattccact tgcctctcat atcctctaga taatcagtat gcggtacagt ccatatggtt
     2701 gagccacagg atttcgcatc gcacttaact acccacctac gcacgcttta cgcccagtga
     2761 ttccggataa cgcttggacc ctacgtatta ccgcggctgc tggcacgtag ttagccggtc
     2821 cttattcatt tggtaccgtc acaatcgtcc caaataaaag cagtttacaa cccgaaggcc
     2881 gtcatcctgc acgcggcgtt gctccgtcag actttcgtcc attgcggaag attccttact
     2941 gctgcctccc gtaggagtct gggccgtatc tcagtcccag tgtggctggt catcctctcg
     3001 aaccagctac tgatcatcgc cttggtaggc cattacccta ccaactagct aatcagacgt
     3061 aagcccctcc tttagcgcat aaagctttaa tcgcaagcga tcacatgcgg tattagcccg
     3121 actttcgccg ggttattccc cactaaaggg caggtactaa cgtgttactc acccgtccgc
     3181 cactaactct taacaagtgt tgatgcctag cagacatatg aaattttcga gaaatgggag
     3241 ctttcgctgc attttcgaaa atttatatgt ctgctctcat caacagcaag aatgtaatta
     3301 caaagtaatt acataagatt aagaattcgt tcgacttgca tgtattaggc acgccgccag
     3361 cgttcatcct gagccaggat caaactctcc aaatagtgct cgtacgaaaa cttttctcga
     3421 ttaaatgaaa taatctcgaa aagtattgta ctcgcattga aatgtttgaa ctggtgatca
     3481 agcccaaaat ttctgcttat acaataagct tcatcttgag acgttgacgt tcattgtctt
     3541 tatagtgcag gacttagccc cacactttca ttttctcaaa gtaaatgatg gttacactgc
     3601 actaccaaaa tttcaacgtc cactctcgaa acgccccaga caaaaaatag ccgaccggtg
     3661 cgggtcgcta tttataggtg gcctggaagt ttttcaagac tagagctagt atatcgtcta
     3721 cgaaaccacc tgtcaacgga ttatgcggct agactcgcat aagccgaatt atgtagctgg
     3781 ctgggatggc cataatcaag cccacaatta aaacggtggt aatcggtgcg accagactaa
     3841 cggctaagag tgtgccaaac agcacaaagc aaccggccgc tccagccatc atcatgccat
     3901 acacatacgg cagcaagggc tcgcgatcgg cattacggta gtagcgcgaa tcatacggag
     3961 tagacattaa cgcgtgacct attccgttaa taaagtttat ccccgcaatt tgtcctgaag
     4021 actgaaccag aattcgtacc gcatttgcca gactaatcgt cagcgtaccc cgctttaagt
     4081 atcctgcggt acccttgcgt gcctggttcc gaccaatata tatggcaatg ataatgctgg
     4141 caattactgc aattgagctt aaaacgccga cacccatata ggatgggacc agcaaaaaaa
     4201 tgaagagcgg ccagatgatt gtgctgaccg aatcatcgat gttgtcgccc atattggcaa
     4261 cgaggtctcg ccatacgcgt cgcaactgca aagcacgaag cttaaatggc tcatgacgaa
     4321 tcatttccga acctccaaat aaagggatgg cggcgaacag gaaacaaagc attgccatca
     4381 tatataagac cgtaataccc aactggctgg ctatcacgcc cccaattgcc ggcgcaatgc
     4441 cgtaagcaca gaggatcaat gccgttgcaa tgccgaccgc tggccccgcc tgtttatgaa
     4501 gcaagctacg cgcaaagcaa gctcgaaagc taggccaata catagcaacg tatagcgccc
     4561 aggtaagcgc gatcaaccat aatggccagt gatagacgga aatggttgcc agcataagaa
     4621 tttgcaaccc atggatgatc atacttgccc ccatcgaccg gttaaagcca atcaaattgg
     4681 cgaaacgcag aaccgcatac tgcatcgccc cccaaaatac tgagaaccag aggtaataag
     4741 ccataatttc cggcacgcta tagccaaaac gatacagata tatcgggata aaaattccgg
     4801 tcatacttga tgcaagcata ctaacggtgt gagcccagta cagccgcttt agctcgctga
     4861 gacggataag atgatagtgg tgcctgccag gatgatgcat catacaggca tcataagctt
     4921 tattagccta tttatatacc tacttccgct ttttggcctt caccgccttg gcacggcgct
     4981 ttttgttagg gccttcaaac catcgctgct tctgaccctc aaccttgtgg gacgccaacg
     5041 ctgacgggag cgaagcataa taggtcctga agattacata cccgcccaag agcaacaatg
     5101 ctaccagcat aatcggccag accacccaaa gagcggcggg taccttttcg tactgaagaa
     5161 aggctaggag gatacatgcg agcccaaagc tccacaatac gtttgaggtg cgattttttg
     5221 cttgacgggc caaggcaatc tcccggtagg aacgcagaag tagccaacga gccgccgccc
     5281 cgaccagaag caggacgaca cccatcgctg ccagtgcata aatataaggc agggttacgg
     5341 gatgaataac cagggcatta aaagcgccca gtttgtcata attgaacaac ccgcctaaaa
     5401 gcagtcccat aaccgcgccg ataaacactt caagcggttt atgtcccaat acttccttga
     5461 gatgagccgg ggctggaagg cggtcgtcat gcttcatgcg atcaattaag gtattgatgg
     5521 caaccgcttg atctccagcc gcacgccgca cgccaaacgc gtcatacata acaattgccg
     5581 ccaagacgat agttaccccg aaaattggcg aacgaggccc ttctaccaag ccggcggtga
     5641 cggctaaagc ggtaaccact gcggcatgta cgctcggcat tcccccggcg gcatagaggt
     5701 atttaaaatc aaccttgcca cgaaacgccg ccacaacgaa cttggtaaat tgggtgatca
     5761 gccagaccag gagcggtact accacgaaac ggttgaacat agattcttaa gtcccctttt
     5821 tcgccttatt gtagcctaaa tggccggtta agggtagatg gattaataga attgtattta
     5881 gcggtcggaa gacttcagtc tacgatccaa gctaagcacc ccgccagcga gcagcatcat
     5941 gaaggctgcg agcacgatca gcaagccggt agaacgcgtg ctggccgcag ctgcatccgg
     6001 gttgtgaata gcggcctgaa cggcgtttgg cagggactgg accgatgcgg ataccgtagc
     6061 aaccgacgca ttatcggcat tagaggcaag gaaggccggc tggatagggc cgctgctacc
     6121 agatccggag ccgctgccca atgtggatgc aaagctggcg ccgcccactc cgagaggagc
     6181 tagctgcagc ggggagaccg gagcagtacc ggctgcagta tcaataccga cgctaccgtt
     6241 ccaggttcca aatacattaa tgaacaagat accgaagact ttcccaaaat gcagcgcgct
     6301 gttgaagatg ttagcgatac tgctagcaat cgtggcattt ccactggtcg cattgccgcc
     6361 ttttgtattg ccgctaacca tcgcatcgcc gctggtggca ttaatcgtaa tgttattggt
     6421 gatactgccg gttaccttat tgtcgatttc agtattattg gtattgctga ccgaggcttc
     6481 attcaccgat cccggaccgg tgttggcgtt ctgcaccacc gcgttcgagg ttagcaaacc
     6541 gccgccggat tcaccggatc cgcccaaatt cataatggtc ccgaaccagc gacccatatt
     6601 gttcacgatt accagaacag cgttatcacc aaaaatgttg gtattaaaga ggttgaacag
     6661 gctactgtgg gtcttggcat tcccggttgt agcactgccg gcttcagtat tgttggctac
     6721 cgcagcggta ccggtttgag cattagcatc aatattatta ttgaatgtag cgtcaacatt
     6781 attatcgatg tcaagggtat tggaatttga attggttgcc gtattagtgg agccaggccc
     6841 ggtagtacta ttgccgagca tagccgtacc cggctggctg ctcgcaacca caccgttaat
     6901 attcaagctc gggaagagaa tatccccatc gagattaccg aagatattaa taatcccgaa
     6961 gaacgactgt ccggcaccga ttgcggtatt aatcaggttt ataatgttga gttcggccaa
     7021 aacatcgccg ctcatcgcgc tgccgccttt ggtgttaccg gtaaccgctg catcgccgct
     7081 agaggcgtca atatcgacat tgttcacaat cgtcccagtg gtctggctgt tcacttcaag
     7141 gttggcactg cagtcatagt cggcaatgtt ggttgagttg ggcccggtgt tggtgttggt
     7201 cgaaaggcct ccggcacatt ggccaagctg gcctcccccg ccagacacgg cttgaggatt
     7261 aatggcaata tcgccggttt ggttgccgta aatattctca acaaagtatg acaagccgcc
     7321 gctgccgaat gtccacatag cattcagtat attaagcaga ttggtgacga tggttgccaa
     7381 accagttttg gcatcgccgg catcggtgtt attactaacc ccggcatcgc cactggcagc
     7441 acccgatgtt atatcgttgt aaattccact attattgagc agggtttcca atacgctacg
     7501 gctatcggtg acacttgctt gattggtgct atttggcccg gtattgctaa tttccggatc
     7561 attcatccct aaaatgcgct tcattagggc agatggatcc ttgctattgg ctgttagggc
     7621 gttaattgaa tcgctcggtg ttacgccggg gatggtatgc caccaaccaa ctactgggtc
     7681 gtagcggtaa aacggcgata cccaattatg ggcgtcctcg ctccatttaa atgagctact
     7741 tatggtaggc tcccattttt gaagggcatc attaaaagca tagtctggaa ctttgcctgt
     7801 aggacccccc ggcccggttg gctcggttgg accggtaaca ccctgaaccc ccgtaggtcc
     7861 gcttggaccg gtaatcggct ggggatcaac aggagggatc ggaggcgcag gggctggcgt
     7921 aggtgtagca gtgctatcta atggagtgtc agctgtttca ttatctgcag gcggaacttc
     7981 agctgacggg tcggcaggcg caaccgtttg aaccggcgga tcatcggacg gatcggcggc
     8041 ctgagctata ccatatggct gggccaacgc cattgtcatt gccaaaacga cggctataat
     8101 gcgctgaaac tgaaatctga aggttgttgc tgtcatatat ttcccctatg cctgcttaat
     8161 ttattgataa tactcatgcc ttggatgagt gccggggagg ggctacctcc cgagggccgt
     8221 gcatacgcga cggcaaattt ggtttggcct ttaccgcggc caagacgtca tcgtaactaa
     8281 attagttagt gatgttaacg gttgtaccgg tgttgttggt gttcg
//
LOCUS       DASVGB010000047         5770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_417133, whole genome shotgun sequence.
ACCESSION   DASVGB010000047 DASVGB010000000
VERSION     DASVGB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417133"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /gene="gatA"
                     /locus_tag="VF272_01470"
     CDS             <1..1330
                     /gene="gatA"
                     /locus_tag="VF272_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX7259581.1"
                     /translation="IAAHEDYHAILEVSAVALDRARAIDTQLKAGKKLGRLAGIPYIA
                     KDNFLTAHTHTTASSNILKPFKAPYQSTVIERLEAEGAIMVAKANLDAFAHGSSTENS
                     DFGPTKNPADPTRVPGGSSGGSATAVALGLTCFALGTDTGGSIRQPAAFTGVVGLKPT
                     YGLVSRYGVIAMGSSVDVIGPLANRVNDAALVLDVTSGKDIHDATTIERDPQAYAGGK
                     PQTLKGKKIGLIKEYMGEGTEAAVKASVEDAAQRLAKAGAKVEEVSLPSVDLALACYY
                     VLIPAELSSNLARHDGIRYGHSSKQAQSLAQTYLLSRDEGFGDEAKRRIMIGTYVLSS
                     GYYDAYYKKAQKVRTLLIREFALAFKKYDFLLSPTAPTTAFKLGEKNQDPLSMYMSDV
                     DTVAVNMVGAAGISIPAPVKGLPIGIQLIAPQHGDRALLGAAKALEELLA"
     gene            1327..1770
                     /locus_tag="VF272_01475"
     CDS             1327..1770
                     /locus_tag="VF272_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259582.1"
                     /translation="MNSLLFLIAILSAGLLILMVIAQSKPPRKRSATSSSHRVGSVDR
                     EFVARKWETIELMSKQGGSGLRDAVSEADKLLDYALKHSGASGETMGERLKHSGKRFS
                     DINAIWSAHKLRNAFAHEADFDLVPGQAREALQNFRQGLKDVGAL"
     gene            1767..3224
                     /gene="gatB"
                     /locus_tag="VF272_01480"
     CDS             1767..3224
                     /gene="gatB"
                     /locus_tag="VF272_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7259583.1"
                     /translation="MKYETIIGIECHVQLATKSKLFCACNNDSRDAEPNVNVCPVCLG
                     FPGALPVLNGAAVRLALKAGHALHAYEKIGEFHSKFDRKNYFYPDSPKNYQITQFDEP
                     IIGKGYVEFPVEGNLKRVGITRAHLEGDAGKLVHPAGADYSLVDLNRSDTPLLEIVSE
                     PDMRTAAEAKAYAQELHNLMRYAEVSDADLYHGNMRFDVNVSLRPVGSKAFGIRTESK
                     NLNSFRAVAGVVEYETKRQAEVLDNGEKVLQETRGWDENNNATFSQRSKEEAHDYRYF
                     PDPDLPPMVITKKMLDDAKDSMPATMPNSLRTSMNNAGIKPEDVETLISDLALSHIFK
                     TATDKQDNEVTVQIVKWLLGEFPHLLTPGTVVELDQARVDVISEGLRELARMQKEGVV
                     SSTAAKQVLAVLVPTGGDPRAIAEKMSLIQVSDSDALEKIVDEVLAANPKPADDYKAG
                     EAKALGFLVGQVMKASKGQANPPMVNAILKKKLGD"
     gene            3226..3786
                     /locus_tag="VF272_01485"
     CDS             3226..3786
                     /locus_tag="VF272_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7259584.1"
                     /translation="MPLFDLQTDKLLEPLSLNLQGALEKVPGALVTLVLGLLVIRLLS
                     YIVSWLMGFVNMPKGLKEILVSLMDALLMIFLIIVVLQKLGLGSLAFALSAFLAAIGI
                     AVGSGSATLVSDILGGITLARDRNFSIGDIVEAGEEKVKGEIVSMDMRRTRIRDNKGQ
                     VHSLPNSVIERKAYTLITKKRDRTDQ"
     gene            3853..4110
                     /locus_tag="VF272_01490"
     CDS             3853..4110
                     /locus_tag="VF272_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259585.1"
                     /translation="MQEVQNILVVMLSIGFAVLLVLGIIMVFILIRIITNIRHITQRL
                     DETTENMGEMAKHVGRKIAPAALSALGSLLVRKAKSKVKRG"
     gene            4117..4620
                     /locus_tag="VF272_01495"
     CDS             4117..4620
                     /locus_tag="VF272_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX7259586.1"
                     /translation="MTHHHKKASKKKGFVTGALVGTLVGGVAALLFAPKSGKETQAEI
                     KQKAQEVVKSGNARLVELGTELDGRIDSLKVAAKELHGEAFEESQRLITRAEILRNDL
                     EASITRLAKTGRDAKQDAAIDAKRLIQDGQDVISELERTTKKIMGSVRERVNRKTDVD
                     KHGESEE"
     gene            4601..4885
                     /locus_tag="VF272_01500"
     CDS             4601..4885
                     /locus_tag="VF272_01500"
                     /inference="COORDINATES: protein motif:HMM:NF040137.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HEX7259587.1"
                     /translation="MANQKSKPTAEDYEKLGRAVEGALINDYINWLRNTKRQIASAFL
                     RGIFTGLGTVIGATLVVALLVWILHMLGGLPGIGNYLKGAGNQIENQVPN"
     gene            complement(4935..5153)
                     /locus_tag="VF272_01505"
     CDS             complement(4935..5153)
                     /locus_tag="VF272_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259588.1"
                     /translation="MADGTALVYWCSPMDVKMICVVMDRRYDHQEEAWRYRLQPLLTK
                     PRVHPFINLYHEVMAWENDLMRITVLKT"
     gene            complement(5186..5470)
                     /locus_tag="VF272_01510"
     CDS             complement(5186..5470)
                     /locus_tag="VF272_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259589.1"
                     /translation="MSDKATAATMIAAGLIASAYRQTESGSVPYTRPNGSPGKFCWNL
                     HSHDGSEPEYWLDENPRHLVGSDPQANQRIRNDGEVLAIHHALNNSGAYR"
BASE COUNT         1504 a         1332 c         1587 g         1347 t
ORIGIN      
        1 gattgccgcg catgaagatt accatgcgat tttagaggtt agcgcagtag ccttggaccg
       61 ggcaagagct atcgataccc agttaaaagc tggaaagaag ttgggacgcc tggcgggaat
      121 accctatata gccaaagata attttcttac ggcacatact cacactacgg cttccagtaa
      181 tattttaaag ccattcaagg caccttatca gtcaacggtc atagaacgct tggaggctga
      241 aggagctatt atggtggcaa aggccaactt ggatgcattt gcccatggat cgtccaccga
      301 gaatagcgat tttgggccta ccaaaaatcc agctgaccct acccgcgtgc ctggaggctc
      361 gtctggtggt tcggcgaccg cggtggcgct tggtttgact tgtttcgctc ttggtaccga
      421 caccggcgga tcaattcggc aaccggctgc cttcaccggc gttgtgggcc ttaagccgac
      481 ctacggactt gtctcccgct acggagttat tgccatgggt agttcggttg atgtaattgg
      541 tccgctggcc aaccgggtga acgatgcagc gctcgtgctt gatgtaacat caggcaaaga
      601 cattcatgat gccaccacca ttgaacgcga ccctcaagcc tatgcaggcg gaaaaccgca
      661 aactttaaag ggaaagaaaa taggcttaat taaagaatat atgggcgaag gaacggaggc
      721 tgcggtgaag gcttcggtag aagatgcggc tcagcgcctg gcaaaagccg gggcgaaggt
      781 agaagaggtg agcttgccaa gcgttgactt ggcacttgct tgctattatg tgttgattcc
      841 ggccgaactc agctcaaact tggcgcgcca cgacggcata cgatacgggc acagctcgaa
      901 gcaggcgcag agtttggcac aaacctatct gttaagccga gacgaagggt ttggggatga
      961 agctaagcga cgcatcatga ttggtaccta cgtgctttca agtgggtatt atgacgccta
     1021 ctataaaaaa gcccagaagg tgcgaacact gctcattcgg gagttcgctc tggcgttcaa
     1081 gaagtatgac tttctgctaa gcccaacggc tccgaccacg gcttttaagc taggcgagaa
     1141 aaaccaagac ccgctttcaa tgtatatgag tgatgtcgac actgtggcag taaatatggt
     1201 gggagccgcc ggtattagta ttccagcgcc ggtcaaaggt ttgccgatcg gcatacaact
     1261 gatcgctccg caacatggcg atcgggcgct cttgggcgca gccaaggcgc ttgaggagct
     1321 attggcatga acagcctgct ctttctgatt gccattcttt cggccggact tcttatttta
     1381 atggtgattg cccagtcaaa acccccgcgt aaacgctcag ctacgtctag ttctcatcga
     1441 gtcggttcag tcgaccgcga gtttgtagcg cgtaaatggg aaactattga gcttatgagt
     1501 aagcagggcg gcagcggctt gcgcgatgca gttagcgaag ccgataaatt gcttgattac
     1561 gctttaaagc attccggagc atctggagag acaatgggtg aacggctgaa gcattcaggg
     1621 aagcggttca gcgatatcaa tgcgatctgg agcgcccata aactacgcaa cgcttttgcc
     1681 cacgaagccg attttgattt ggtgcccggc caagcgcggg aggccctcca gaactttcgc
     1741 caggggctga aagacgtggg ggcgctatga aatacgaaac cattatcggc atcgagtgcc
     1801 atgtacagct ggctaccaag agtaagctgt tttgtgcctg caataacgat tcacgtgatg
     1861 ccgagccgaa cgtcaatgtt tgtccagtat gcctggggtt tccgggtgcc ttgccggtgc
     1921 tcaatggggc ggcggtgagg ctggcactca aagccggcca tgccttgcat gcctatgaaa
     1981 aaatcggcga gtttcattcg aaatttgacc gaaaaaacta tttttatccc gatagtccta
     2041 aaaactacca aatcacccag tttgatgagc caattatagg gaaggggtat gtggagtttc
     2101 cggtagaagg aaacctgaaa cgagttggta ttacccgtgc acatttggag ggcgatgcgg
     2161 gtaagttggt gcatccagcc ggagcagatt actcgctggt tgacctgaat cgttccgata
     2221 cgcccttact ggaaattgtt tccgagccgg atatgcgaac ggcagcggag gctaaagcat
     2281 atgctcagga attgcataac ttgatgcgct acgccgaggt gagcgacgca gacctctacc
     2341 acggcaacat gcgcttcgac gtaaacgtga gcttacgacc ggttgggtca aaagcgtttg
     2401 gcattcgcac tgagagtaaa aatttaaact catttcgagc ggtagcgggt gtagttgaat
     2461 acgagaccaa gcggcaagcc gaggtgctcg acaatggaga aaaggtttta caagaaaccc
     2521 gcggctggga tgagaataac aatgcaacct tttcccagcg cagcaaggaa gaagctcatg
     2581 actaccgcta tttccccgat cctgatctcc cgcccatggt aataaccaaa aaaatgcttg
     2641 atgatgctaa ggattcgatg ccagcaacca tgcccaacag cttgcgcacc agtatgaaca
     2701 atgctggcat aaagccggaa gatgtggaga ccttgattag cgatttagcc ctgtcgcata
     2761 tctttaaaac agcgaccgac aagcaagata atgaagttac cgttcaaatc gtaaagtggc
     2821 ttcttggaga gttcccgcat cttcttacgc ctgggacggt ggttgagctt gaccaagcgc
     2881 gggttgatgt catttccgaa ggtctcaggg agttagcccg aatgcaaaag gagggagtcg
     2941 taagctcaac agctgctaag caagttctag cagtgttggt ccctaccggc ggcgatcctc
     3001 gtgcaatcgc tgaaaaaatg agcttgattc aggtttccga ttctgatgcc ctggaaaaaa
     3061 ttgttgatga agtgctggct gccaacccaa aaccagcaga tgattataag gcgggggagg
     3121 cgaaagcctt aggattttta gttggccagg tgatgaaagc ctcaaaagga caggctaatc
     3181 cgcctatggt taatgctata ctgaaaaaga aattaggaga ctagcatgcc gctattcgat
     3241 ctacaaacag acaaactatt agaaccacta tcattgaacc tacagggcgc actggaaaaa
     3301 gtacccggcg ccttggtgac gctggttctt ggtttgctgg taattagatt actttcatac
     3361 attgtaagct ggctgatggg tttcgttaac atgcccaagg gcctaaaaga aatcctggtg
     3421 tccctgatgg acgcgctttt gatgattttc cttatcatcg tagtgctgca aaagctgggt
     3481 ttaggctcgc tcgccttcgc cttatcggcc ttccttgcag ccatcggtat tgcggttggt
     3541 agcggctcag ccactcttgt gagcgatatt ctaggcggca taaccttggc ccgcgaccgt
     3601 aatttcagta ttggcgatat tgttgaagcc ggtgaagaaa aggtaaaagg cgaaattgtc
     3661 agtatggata tgcgccgcac gcggattcga gataataagg gacaagttca cagtttgcca
     3721 aattcggtaa ttgagcgaaa agcctacacc ctcataacaa agaagcggga tcgtactgat
     3781 cagtagcagt agtgcttggg cttgcatatc aggcccagag tacggatact agaagcgtaa
     3841 caaggccata ttatgcaaga agtacaaaac attctggttg taatgctaag cattggattt
     3901 gctgttcttt tggttctggg tattattatg gtatttattc ttattagaat aattaccaac
     3961 attcgtcaca ttacccagcg ccttgatgag actaccgaaa atatgggcga aatggcaaag
     4021 catgtcgggc gcaaaattgc tccggcagct ctttcagccc ttggatcact attggttcgc
     4081 aaggctaagt caaaagtaaa gcgagggtaa tagaacatga cacatcatca taaaaaggca
     4141 agcaaaaaga agggattcgt aaccggagcg ctggtgggta cgctggttgg cggggttgct
     4201 gcattattat ttgcgcctaa aagcggcaag gaaactcaag cggaaatcaa acagaaagcc
     4261 caggaagtag taaaatccgg caatgcccga ttggttgaac tgggcaccga gctcgacggt
     4321 cgaattgata gcctgaaagt cgcggccaaa gagcttcatg gcgaggcatt tgaagaaagc
     4381 cagcgtttga taacccgggc tgaaatactg cgcaatgacc tagaagcatc aattacccgc
     4441 ctggcaaaaa ccggacgcga cgccaagcaa gatgctgcga ttgatgctaa gcgcttaatt
     4501 caagacggcc aagatgtaat ttccgagttg gagcgaacta cgaaaaaaat tatgggttcg
     4561 gttcgtgaac gggttaaccg aaaaaccgat gtagataagc atggcgaatc agaagagtaa
     4621 acccaccgcc gaagattatg aaaagttggg ccgcgccgta gaaggtgcgc tcattaatga
     4681 ttacattaac tggctgcgca atacaaagcg gcagattgct tcagccttcc tacgcggcat
     4741 atttaccggt ttagggacgg taatcggagc tactttagtg gttgcccttt tggtgtggat
     4801 attgcatatg ctgggcgggc ttccgggaat tggcaattat ttgaagggtg ccgggaatca
     4861 aattgagaac caggtgccga attaaattgt aattagtgcg gtcaggcgac ccgccatccg
     4921 aagatggcag gtcgctaggt tttgaggacg gtgatcctca taaggtcgtt ttcccacgcc
     4981 atcacctcgt gataaaggtt gatgaagggg tggacccgcg gcttggtgag cagtggctgc
     5041 agacgatacc gccaagcctc ttcttggtgg tcatagcgcc tatccatgac aacgcagatc
     5101 atcttgacgt ccatcggtga gcaccagtag accagtgcag tgccgtcagc caaaaccttc
     5161 ggcggtggcc aatgaggcat taccgctacc ggtatgcgcc ggagttgttc agggcatgat
     5221 ggatggccag aacctcgccg tcgttgcgga ttcgctgatt ggcctgggga tccgacccaa
     5281 cgaggtggcg cggattctca tcaagccagt actcgggctc gctgccgtcg tggctgtgaa
     5341 ggttccaaca gaacttgcct ggcgaaccgt tcggccgggt gtagggcacg ctgccggact
     5401 ccgtctggcg ataagcgctg gcgatcaagc ccgcagcgat cattgttgcg gccgttgctt
     5461 tatcagacat tactcctgct ttcgcattgc gggttggtaa gtcagtttgt tattaataac
     5521 acaaaatcaa caatctttca agtgtaattg tcaataacgc taatgctgcc ccgtggtata
     5581 ctgaatgaat cgaaaagggg aagcgtttga gcgaagatac ggctgcaccg acgccattgc
     5641 cggcgtcaaa atcgaagttt gtgcatctgc ataatcacgg ccattatagt ttgctggacg
     5701 ggctgcagaa ggtgccgcag atgctagatc gagcagccga actgggcatg gatgcaattg
     5761 cgcttaccga
//
LOCUS       DASVGB010000048         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1172377, whole genome shotgun sequence.
ACCESSION   DASVGB010000048 DASVGB010000000
VERSION     DASVGB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172377"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VF272_01515"
     CDS             complement(<1..238)
                     /locus_tag="VF272_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259590.1"
                     /translation="MSALPAFSIREATLDDLPAIQAMSYELFIDPTSSGDSLLDSTWS
                     KDERGEKYFRTWIENSTDTMLVAESPNELVGFLSG"
     gene            complement(243..1760)
                     /gene="rny"
                     /locus_tag="VF272_01520"
     CDS             complement(243..1760)
                     /gene="rny"
                     /locus_tag="VF272_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX7259591.1"
                     /translation="MEVAVLVATLAAGIAGGYGGSVYYAKRKTNTAEAKAKGLIKDAQ
                     SKAQELTLAAKEQALGIAEEAKKEEKERRQQMTEIENRVSQRETTLDRKLEELDKRSE
                     SLRKSETSLEELKDDLRKLRAKQQESLEKIAKLSKDEAREKLIKMVEKDTKADLTGLI
                     AKLQQEAVESADEQAEMVLVTAMERMASDQASERTVTTVALPSDELKGRIIGKEGRNI
                     QTIERLTGVDVLVDDTPGVIVLSSFDPIRRQVARVTLEKLLADGRIHPARIEEVVTKA
                     HSEIDKIVKEAGEAAVKEAGVVGLHPELIKLLGQLRFRTSYSQNVLKHSVEMSALAAT
                     IAQEVGADVRVVKTASLLHDLGKAVTHEIEGQHHHISRDMAAKYGQDEAVCHAIEAHH
                     DDIEATTPEAIIVRIVDALSGGRPGARGDTMENYVKRMTELENLANTFPGIQKSYAVS
                     AGRELRVLVVPEEIDDLSAIKLARDLAIKIEATLKYPGVIKVNIIRETRAIEYAK"
     gene            complement(1917..>2234)
                     /gene="tsaB"
                     /locus_tag="VF272_01525"
     CDS             complement(1917..>2234)
                     /gene="tsaB"
                     /locus_tag="VF272_01525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX7259592.1"
                     /translation="EWDAGRQLSKQLLSRIETLLQTQKASWDDLSGIVVFRGPGSFTG
                     LRIGITVANTIAYAKLIPIVGEIGERWSDNGIKRLIAKDNDRQVLPHYGAPANITKPR
                     GTA"
BASE COUNT          479 a          607 c          529 g          619 t
ORIGIN      
        1 cgccagacaa aaacccaacc aactcattag gtgactcggc taccaacata gtatccgtgg
       61 aattttcaat ccaagtgcgg aaatacttct cgccgcgctc atctttcgac cacgtactat
      121 ccagtaagga atcgccgcta cttgtcgggt ctatgaacaa ttcatagctc attgcttgaa
      181 tcgctggcag atcgtcaagc gtcgcctcgc gtattgaaaa ggctggtaga gcgctcatgc
      241 tgctacttag cgtactcgat cgctcgggtt tcgcggatga tattcacctt aatcacacct
      301 gggtatttca atgtggcttc aatcttgatg gccaggtcgc gggccagctt aatggccgat
      361 aggtcgtcaa tttcttccgg tactaccaaa acgcgcagtt cacgtccggc cgaaaccgca
      421 taacttttct gaatgccggg aaacgtattg gccaaatttt ccagctcggt catgcgtttc
      481 acatagttct ccatggtatc accgcgggca cccgggcggc ctccggagag agcgtcaaca
      541 atccgtacaa taatcgcttc cggcgttgtc gcttcaatat cgtcgtggtg agcctcaatg
      601 gcatgacaga ccgcttcatc ctggccgtat ttagcggcca tatcacggga gatgtgatga
      661 tgttgacctt caatttcgtg agtaaccgcc ttgccgaggt cgtgcagcag cgaagccgtc
      721 tttacaaccc tgacatcggc gccaacttct tgggcaatgg tggcagctaa ggccgacatc
      781 tccactgaat gctttaaaac attttgacta taggaagtac gaaaccgcaa ctgtccaagc
      841 agcttaatta gttcggggtg caatcctaca acgccggctt ctttaaccgc cgcttcgccg
      901 gcttctttaa caatcttatc gatttccgaa tgggcttttg tcactacttc ctcgatgcgg
      961 gcagggtgaa tacggccatc agctaacagc ttttccaggg ttacccgagc cacctggcga
     1021 cggattggat cgaagctgga gagcacaatt acacccggtg tgtcgtcaac cagcacatcc
     1081 accccggtaa gccgctcgat ggtttgaatg ttgcggcctt ctttgccgat aattcttcct
     1141 tttaactcgt cgctgggcag agcgaccgtg gtgacggtgc gttcgctggc ctggtcggaa
     1201 gccatgcgct ccatggcggt aactaagacc atctcggctt gctcgtcggc actttctacc
     1261 gcctcttgct gcaacttggc aattaagccg gttagatcag ccttggtgtc tttttcaacc
     1321 atctttatca gcttttcgcg ggcttcatcc ttgctcagtt tggcaatctt ttccaagctt
     1381 tcttgctgtt tcgcccgaag cttgcgcaga tcatccttta gttcttcgag actggtttca
     1441 cttttacgaa gcgattcgga acgcttatca agctcctcca gtttgcggtc gagcgtggtt
     1501 tcacgttggc tcacgcgatt ttcaatttcg gtcatttgct ggcggcgttc tttttcttct
     1561 tttttagcct cttcggctat gcccagtgcc tgttctttcg cagccaacgt tagttcttga
     1621 gccttagatt gggcatcttt aatcaagcct ttggccttgg cttcggctgt gttggttttt
     1681 cttttggcgt agtaaacact tccaccgtaa ccgccggcaa tgccagccgc tagagtggcg
     1741 acgagtacag caacttccat aacgcctcct caggcgctga aaatgcagac actttaacag
     1801 cactgaacaa aactaacttg tgatatcagg tatcagcgtg cgcggcggtc tgctaggaat
     1861 cagcacgttc atttattaag cagtacctat attaaacccc ggggagtaaa tgctcgtcaa
     1921 gctgtgcctc ttggtttggt aatattggct ggcgctccgt aatgcggcaa aacctgccga
     1981 tcgttatctt tagcgatcaa acgcttgatg ccattatcgc tccaacgttc accgatttcg
     2041 ccaacgatcg gaatcaattt tgcatacgcg atggtgttag ctacggtaat tccgatgcga
     2101 agtccggtaa atgatcccgg cccgcgaaat accacaatac cgcttaagtc atcccatgag
     2161 gccttctgag tttgcagtaa cgtttctatc cggctgagaa gttgttttga aagctggcgc
     2221 ccagcatccc attc
//
LOCUS       DASVGB010000049         4090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_712457, whole genome shotgun sequence.
ACCESSION   DASVGB010000049 DASVGB010000000
VERSION     DASVGB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712457"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VF272_01530"
     CDS             <1..910
                     /locus_tag="VF272_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX7259593.1"
                     /translation="WSLKFRRSDVQRVVKLMVPRSLDQGIDQIHYIVETIIASRLQVG
                     SLAIYYFANNLKNVPLVLIGNSVATAAFPTMASRAAKGAYETLIKEFVINARLILFLV
                     VPAAIVTVIMRGYIVRLLYGFGDPTTANTLGWFAGAIIFQALFFLVARMFYALQDSKT
                     PLYVSVSAIIINIPLSIGLSNEFGVVGLAMAQSVLAVLEVVVLTILLQRRMEQIGLES
                     IWAGLWRMLLAGAIMIGSTYIVVARVMPLYATDLGFIVVAPKFFGILAISLAAYFIPC
                     YLLDLHEARKVYGKLKEQILKPLNLT"
     gene            910..1158
                     /locus_tag="VF272_01535"
     CDS             910..1158
                     /locus_tag="VF272_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX7259594.1"
                     /translation="MEAYVGAAAFLLTLVIQLPQAVKVVRTHQTRDLSILTYVLLMIT
                     AGLWIAHGIIRGDLAVWTANIMVLLLSAIILGYKLKFG"
     gene            1160..3031
                     /gene="lepA"
                     /locus_tag="VF272_01540"
     CDS             1160..3031
                     /gene="lepA"
                     /locus_tag="VF272_01540"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX7259595.1"
                     /translation="MNQSHIRNFCIIAHIDHGKSTLADRMLELTGTVAGRDMQAQLLD
                     SMDLEREKGITIKLQPVQMAWTLGSMRSSEGRGLSEPKASSAAEDDARDVGTYQLNLI
                     DTPGHVDFSYEVSRSLAACEGAILVVDAAQGIQAQTLANVYLAIEAGLEIIPVLNKID
                     LPAAEPDKVAAEVAVLLGCQPGDVLRISAKEGTGVRELLDKVVEQVPPPSGYPQKPLR
                     GLVFDSVYDEYRGVILFLRIVDGSLKKQAGLKLMSSGHTGLAVETGIFKPKQVIQPEL
                     TTGQIGYVVTNLKSIAEARVGDTVTLERESAGQALPGYRAVKPFVFAGFFTSSAEQYP
                     DLKDALEKLKLNDAALVYEPESSQILGFGYRVGFLGLLHMEIIKERLEREHGLDMVVT
                     TPSTDYQVRTSDGQEMTVRSAAELPETSRIELLREPWIKGEVVVPGEYVGPVIQLINR
                     IRGLQSTMNYIDQTLALINFEAPLANVLTNFYDSLKSITSGYGSFNYELDDYRTEDLV
                     RLDILVGGELVDSLSSIVHREEAYGRGKAIVDKLKEVVPRQLFEVSLQAAIGGKFIAR
                     ENIRAMGKNVTAKLYGGDVSRKNKLLDKQKKGKKRMKKVGKVDIPAEAFMVLVKADQ"
     gene            3051..>4090
                     /locus_tag="VF272_01545"
     CDS             3051..>4090
                     /locus_tag="VF272_01545"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7259596.1"
                     /translation="MSYTPPQNILENYAKVLVNFALNGGQGLRKGEVVYVMGHEVSKP
                     LYVEVCKAVWRSGGHVIDGFFPDEAGRYGGLNDIYDLASDEQLDFFAAKYYKGLTDEM
                     DHQLMILSDTDLHALEKVDPAKILRRGKARQPYMQWRDEKENAGKFTWTLALYGTVAA
                     AAEAKLSEEEYWEQIISACFLDEADPVAKWKETEAKIKEYVMKLNELDIEKVHVEGDD
                     VDLWVKIGEQRRWAGGSGRNIPSFEVFTSPDWRGVEGWIKFNVPLYAYGSMVEGIRLE
                     FKAGKVVNATATNNEPVLTHMIATENADKVGEFSMTDRRLSRITKFMANTLYDENIGG
                     PFGNSHIAVGKA"
BASE COUNT          999 a          904 c         1147 g         1040 t
ORIGIN      
        1 ttggtcgctg aagtttagga gaagcgatgt tcagcgggta gttaagctaa tggtgccgcg
       61 gtcattagac caaggcattg atcaaattca ttacattgta gaaaccatta tcgcctcacg
      121 cctccaggta ggtagcttgg ctatttatta ttttgccaat aaccttaaaa acgtcccttt
      181 ggtattaatt ggaaattcgg tggcaacagc agcctttcca accatggctt cacgggcagc
      241 caagggagca tatgaaactc ttattaagga gtttgtgata aatgctcgcc ttatattgtt
      301 cctggtggtg ccagcggcaa ttgtaacggt gattatgcgg gggtatatcg tgcgtctgct
      361 gtacggattc ggtgacccca ccaccgccaa taccttgggt tggtttgccg gagctataat
      421 cttccaagca cttttctttt tggttgctcg catgttttat gcgttgcaag atagcaaaac
      481 accgctctac gtcagcgtat cggccatcat cattaacatt ccgctgagca ttggactgtc
      541 gaatgagttt ggcgtggttg gactggctat ggctcaatca gtacttgccg ttcttgaagt
      601 tgtagtctta accatccttt tgcagcgccg catggagcag attggcttgg agtcaatttg
      661 ggcgggactg tggcgcatgt tgcttgccgg agcaattatg attggtagta cctatattgt
      721 ggtggcccgt gtaatgccac tctacgccac cgaccttggg tttattgtag tggctcccaa
      781 gttcttcggc attctcgcca tctcgctagc ggcctatttc atcccatgtt acttacttga
      841 cttacatgaa gctcgcaaag tatacggcaa gctgaaagaa caaattctta aaccattgaa
      901 cctaacctag tggaagctta tgttggagca gcagcctttc tgctgacctt ggttatccag
      961 ctgccacagg ccgtaaaagt ggtacggacg catcagactc gggatttatc gatcctaacg
     1021 tatgtattgc ttatgattac cgctggactc tggattgctc acggtattat tcggggtgac
     1081 ttagcagtgt ggactgccaa cattatggta ttgttgctga gcgctattat tctcggctat
     1141 aagttaaagt ttggataaga tgaatcagtc tcacattcgc aatttttgca ttatcgcgca
     1201 tatcgatcat ggcaaatcga cgttagccga tcgtatgttg gaactgaccg gtacggttgc
     1261 cggccgggat atgcaggctc agttgctgga tagtatggat ctggagcgcg agaaggggat
     1321 aaccattaag ctgcaaccgg tgcagatggc gtggacgctc ggatcgatgc gctcatctga
     1381 agggcgagga ctgtctgagc cgaaggcgag ttccgcagcc gaagatgatg cgcgcgatgt
     1441 aggcacttac caattgaatc tgattgatac gcccggacat gttgatttca gctatgaagt
     1501 ttcgcgcagt ctggctgcat gtgaaggtgc gattttggtg gtggatgcgg cccaggggat
     1561 tcaagcccag acattggcga atgtgtattt ggcgattgaa gccggactgg aaattattcc
     1621 ggtgctcaat aaaattgacc tgccggcggc tgaaccggat aaagtggcag ctgaggtggc
     1681 ggttttgctt ggatgccagc cgggcgatgt cctgcgtatt tcagccaagg aagggacagg
     1741 cgttagggag ctgctggata aagtggtcga gcaagttccg ccgcccagcg gatatccgca
     1801 gaagccgttg cggggcttgg tgtttgattc ggtctacgat gaataccgcg gcgtgattct
     1861 gtttttgcgg attgtcgatg gttcgttgaa gaaacaggca ggcctgaaat taatgtcgtc
     1921 tggtcatacc ggactggcgg ttgaaaccgg catctttaag cccaagcaag ttattcagcc
     1981 cgaactcacc accgggcaga ttggctatgt ggttacaaat cttaaatcca tcgctgaggc
     2041 acgggtgggg gatacggtaa ccttggaacg cgaatcagct gggcaggcac tgccgggcta
     2101 ccgggcggtt aagccatttg tcttcgccgg attctttacc tcgagcgctg aacagtatcc
     2161 cgatctaaaa gatgccctcg aaaaattaaa attgaatgat gcagcgctcg tgtatgagcc
     2221 ggagagttcg cagattctgg gttttggcta ccgggtcggc tttttagggt tgctacatat
     2281 ggaaattatt aaagagcggt tggagcgtga acacgggttg gatatggttg tgaccacacc
     2341 atcaaccgat tatcaggtac gcaccagtga cggtcaggaa atgacggtgc gttcggctgc
     2401 tgagctgcca gaaaccagtc ggattgaact attgcgtgag ccgtggatta agggtgaggt
     2461 tgtcgtgcca ggcgaatacg tcgggccggt gattcagttg attaaccgta tccgcggcct
     2521 gcagtcaact atgaactaca tcgatcaaac cctagcctta attaattttg aggcgcctct
     2581 agcaaatgtg ttgaccaact tctacgattc gctgaaatca atcacgagcg ggtacggttc
     2641 cttcaattat gaattagatg attaccgcac cgaagatctg gtgcgcctgg atattttagt
     2701 cggcggcgag ctggtagata gtttgagttc tattgtccat cgcgaagaag cttatggccg
     2761 cggaaaagcc attgtcgata aactaaaaga agtggtgcct cggcagttat tcgaagttag
     2821 tttgcaggca gcaatcggcg gtaaatttat tgcccgtgaa aacattcggg ccatgggcaa
     2881 gaacgtcacc gccaaactct acggcggcga tgtctcgcgc aaaaataaat tactggacaa
     2941 gcaaaaaaaa ggcaaaaagc gcatgaagaa ggttggcaaa gtcgacatcc cagccgaggc
     3001 cttcatggtg ctggttaagg ccgatcaata ggtttgctac aatagagttc atgtcgtata
     3061 cccctcccca aaacattctc gaaaactacg ctaaggttct ggtgaacttc gcccttaatg
     3121 gtggtcaagg cctgagaaaa ggagaggttg tttatgtgat ggggcacgag gtttccaagc
     3181 cgctctacgt tgaggtctgc aaggcggtgt ggcgttccgg cggtcatgtg atcgacggct
     3241 ttttccccga tgaggctgga cggtatggcg ggttgaatga tatctacgac cttgcctcgg
     3301 acgagcagct tgatttcttc gcggctaagt actataaagg cctgaccgac gaaatggatc
     3361 atcaactcat gatccttagt gataccgatc tgcatgcgct ggaaaaagtt gatccggcaa
     3421 agattttacg ccgcggtaag gcccgtcaac cgtacatgca gtggcgggac gaaaaggaga
     3481 acgccggcaa attcacctgg acgcttgcct tatacggtac ggttgcggca gcggcagaag
     3541 caaagttaag cgaagaagag tactgggagc aaattatcag tgcctgcttt ttagacgagg
     3601 ccgacccggt agccaaatgg aaggaaaccg aagccaagat taaggaatac gtcatgaaac
     3661 tcaacgagtt ggatattgaa aaggtacatg ttgaaggcga cgacgtggac ttgtgggtca
     3721 agatcggcga gcaacgccgt tgggctggcg gcagtggccg gaacatcccc agttttgaag
     3781 tgttcaccag tcctgactgg cgcggcgtcg agggatggat caaattcaac gtgccgctgt
     3841 atgcttatgg cagcatggtg gaggggattc gcctggaatt caaagctggc aaagttgtga
     3901 atgctaccgc cacaaataat gagccggtat taacgcatat gattgccacc gagaatgccg
     3961 acaaagtcgg ggagttttcc atgaccgata ggcggctttc acgcattact aaatttatgg
     4021 ccaatacgct ttatgacgaa aatattggcg gaccgttcgg caacagccac attgccgtcg
     4081 gcaaggctta
//
LOCUS       DASVGB010000050         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_416707, whole genome shotgun sequence.
ACCESSION   DASVGB010000050 DASVGB010000000
VERSION     DASVGB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416707"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF272_01550"
     CDS             complement(<1..323)
                     /locus_tag="VF272_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7259597.1"
                     /translation="MSQLRVAMIAPPWLTIPPKGYGGIEYVLHGLVQELEKRGVQITL
                     FTVGDADISVFDQKSIFKKDMHPYLGLPFYEAAVIPLSHITFALNQIKDNGFDIIHDH
                     NPGVGA"
     gene            510..2411
                     /locus_tag="VF272_01555"
     CDS             510..2411
                     /locus_tag="VF272_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259598.1"
                     /translation="MQLQNNLWIVNPFYIDIDPLKQLRIVGPGNHLFFGSAGLYAPDF
                     EVISVKSKSSNLIMELQLKASAIELHDPVMGPIRRIRQHFEIAGSPRCPYIDAAANPG
                     SDLFPNFVSVTIQASGATITYHRRYADQWYGIVFRLGENCKVERDENARTIKLSSAKP
                     ILITLETSSSSTFKPEVASVLKTTPINLEPFGDQAKEIKQLLDRTETEINHLVTTNKT
                     SGFEYGTVFPRDWMEAASLGQIDLTDTGIQYMYRKSLEHVSSEGHGWHEDIVGEFKNE
                     HEMELQGLSNMLEEMVDPERAASIKADIQKLQQYQITRHMIDIEPLYILGLKWISPKQ
                     LSEDEVQRLRTVAWYVLDQATTNEIITFKELPRLLRRHREEQYYTAGNWRDSEWAYRQ
                     IHPKLAPYDVNAVFYPEALKLMHSHASLLGLDAAEVKAVAKKWKDKKKHFRFINPDGS
                     TAYALALYDIQSLEPDKYQMLKINHLDEAYDLFYGQPSQEDIISFTRRLTNPNYFYTP
                     SGPLLVGRNQGYDNRFYHGEVIWTKQTAFTVAGLEHQMKRAKAEKWPLELLMQLQEAL
                     EITTKASVSAFLTLGAVPELHYDDNGRPRLYTDQPAAEGVMNLVQLWSAMGARRIIRS
                     YVTARNKPN"
     gene            2467..2901
                     /locus_tag="VF272_01560"
     CDS             2467..2901
                     /locus_tag="VF272_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7259599.1"
                     /translation="MKLNQGTTYQIGLIQAQAYRRLQNHYHAALEPYGISIPEWSLLG
                     MLRDAGSMTPSELAKILKSKVSHPSALIAKLEKRGLVKRRADGEDKRSTHIALTAAGA
                     KLVPAVENHVRATLRHDLKDLNAAELVLYYRLLAKLAKGFEE"
     gene            complement(2904..3653)
                     /locus_tag="VF272_01565"
     CDS             complement(2904..3653)
                     /locus_tag="VF272_01565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7259600.1"
                     /translation="MLTSAVVLIGLQLKPMGWSLLLLAGFSLLLADKLFAKDMLLIVI
                     ALAILGITPITTDVSYSHMAVMGAALLLALAIPYGVSRFVYGDYRVRFRFHHGRSWYK
                     TEVAYIAVAALISYLIIPFYLTSTGVYMNWPAADSSSVARLFTGTNVLGIWDELFFIS
                     TILGLFRHYFKFHLANVFQAVLFTAFLYELGFQGWGPVMIFIFALLQGYVFKKTESLF
                     YVITIHLTVDVVLFLALMHTHHPDMFAIFIT"
     gene            complement(3740..4291)
                     /locus_tag="VF272_01570"
     CDS             complement(3740..4291)
                     /locus_tag="VF272_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7259601.1"
                     /translation="MNLKNQRGIAYLPLILPVAVILLGAGTFVAVQQQTAKKTVSASI
                     VRHGGDDANRTTAQTPTITLTSAKSIAMAQHPGSTIRKVELETEDGVLVYSVRFVDGA
                     RVDVRASTGAIERNEPATETNTTGGDASNTTNSNSGPGNVNSDDNNDAMDHDFNDNND
                     FNDNEDGSDDEGIDHHRRGSGRH"
BASE COUNT         1277 a         1104 c          993 g         1081 t
ORIGIN      
        1 gcccccaccc cagggttatg atcatgaata atatcaaacc cgttatcttt aatttgattc
       61 agagcaaacg ttatatgcga taaggggatt accgctgcct cataaaatgg gagtcccagg
      121 tacggatgca tatctttctt aaaaatcgac ttttggtcaa ataccgaaat atcagcgtca
      181 cctactgtaa ataaggtaat ttgtaccccc cgtttctcca gctcctgtac tagtccatgg
      241 aggacgtact caatacctcc ataacctttc ggtggtattg tcagccatgg tggtgcaatc
      301 attgcaaccc gcagttgact catatccata ctcctttccc ttgtactact gctccgcgaa
      361 atgcttcaac agtcacaaca tcataccggt aatactgttg caagccattg gctaaaagcc
      421 caaaaagcag tgaatatacg ttactcagta gtggaattac acgaagcaga aagcggtcca
      481 accgtatatc gtagagatgg cggcaaccta tgcaattaca gaacaatctc tggatcgtta
      541 atccttttta catcgatatc gacccgctta aacaacttcg cattgtcgga cctggcaatc
      601 atcttttttt tggtagtgcc ggtttatacg caccggactt cgaggttata tcggtaaagt
      661 caaaatcgtc taacctaata atggagctcc agctaaaagc ctcggctatc gagctccacg
      721 atccagtaat gggccccatc cgccggatcc gtcagcattt tgaaatagcc ggcagcccca
      781 gatgtccata cattgatgcc gccgccaacc ctgggagcga tctgttccca aactttgtct
      841 cagtaactat ccaagcctcc ggagccacca ttacctatca tcgccgttat gccgaccagt
      901 ggtacggcat tgtgtttcgg ctgggagaga attgcaaggt tgagcgtgat gagaacgccc
      961 gaactattaa gctgagttcc gcgaagccga tactgatcac cctggaaacc agcagctcgt
     1021 cgacgtttaa accggaggtt gcatccgtgt taaaaaccac gcccatcaat ctcgaaccgt
     1081 ttggggatca agcgaaggaa attaaacagt tattggaccg caccgaaacc gaaataaatc
     1141 acctggtaac cacgaataaa accagcgggt tcgaatacgg cacggttttc ccacgtgatt
     1201 ggatggaagc tgcgagtttg ggccagatcg atctcactga cacgggaatt cagtacatgt
     1261 atcggaaatc actggaacat gtgagcagtg aaggccatgg ttggcatgaa gatattgtcg
     1321 gcgaattcaa aaatgaacat gaaatggagc ttcagggtct ttccaatatg ctggaggaga
     1381 tggttgatcc tgaaagagcg gcctccatta aggccgatat ccaaaaactt cagcagtatc
     1441 aaatcacccg ccatatgata gatattgaac cgctctatat tttaggtctg aagtggattt
     1501 cgccgaaaca gttaagcgaa gatgaggtgc aacgattaag aacggtagcc tggtacgtgc
     1561 tcgatcaggc cacaacgaac gagattatta cttttaagga gctcccccgc ctattgcgac
     1621 gccaccgcga ggagcaatac tatacggctg gcaactggcg cgacagcgag tgggcctacc
     1681 gccaaatcca ccctaaactg gctccctatg atgtgaatgc cgtattttac cccgaggcac
     1741 tcaagctaat gcattcgcat gcctccctgc ttggtcttga cgctgccgaa gttaaggctg
     1801 tggcaaaaaa gtggaaggat aagaaaaaac atttccgctt tatcaatcct gatggctcaa
     1861 ccgcatatgc cttggcgcta tacgacatcc aatcattaga acccgataag tatcaaatgt
     1921 taaaaattaa tcacctagac gaagcgtatg atttatttta cggtcagcca agccaggaag
     1981 atattataag ttttacgcgc cggctaacca atcccaacta tttttacacc ccttcaggcc
     2041 ctttgctggt gggtcgaaac caaggctatg ataaccgttt ttatcacggc gaagtcattt
     2101 ggaccaagca aaccgcattt acggttgcag gcttagagca tcaaatgaag cgggccaagg
     2161 cagaaaagtg gccactggag ctcctcatgc aacttcaaga agcactggaa attactacca
     2221 aggccagcgt ttctgccttc ttaacgcttg gcgctgttcc agaattacat tatgacgata
     2281 acggacgccc ccgcctctac accgatcaac cggccgccga gggcgtcatg aatctagtcc
     2341 aactttggag cgccatgggt gcccggcgaa ttatccgctc gtacgtaacg gcacggaata
     2401 aaccgaatta gtcctggcgt tatgattgaa aaatggtgac gcatgtggta atttctaaac
     2461 tcacttatga aactcaatca aggcacaacc taccaaatag gcttgattca ggcacaagcc
     2521 taccgtcgcc tgcaaaacca ttatcatgca gctcttgagc cttatggaat aagtattccc
     2581 gaatggtcgc tcctgggaat gttacgcgat gcggggtcta tgacgccaag tgaactggct
     2641 aaaattctta aaagcaaggt ctctcaccca tctgcattga tcgccaaact ggaaaaacgc
     2701 ggattagtaa aacgcagagc cgacggtgaa gataagcgca gcactcatat agcattgact
     2761 gcggcgggcg caaaacttgt tccagccgta gaaaaccatg tacgcgccac ccttcgacat
     2821 gatttaaaag atttgaacgc agccgagctt gtgctgtact accgcttgtt ggctaagctg
     2881 gctaaaggat ttgaagagta agcttacgtt ataaaaattg caaacatgtc aggatggtgg
     2941 gtatgcatca acgccaaaaa aagaactaca tcaacggtta gatgaatcgt gattacataa
     3001 aataatgact cggttttttt aaagacgtac ccctgtagaa gcgcaaatat aaagatcatt
     3061 accggtcccc atccttgaaa ccccagctca tacagaaacg cagtaaataa aactgcctgg
     3121 aacacatttg ccagatgaaa tttaaagtaa tgacgaaaca atcccagaat agtactaata
     3181 aagaatagct cgtcccagat acctaagaca ttggtcccag taaatagcct ggcaacactc
     3241 gatgaatcgg cggcgggcca attcatatag acgccggtag aggttaagta aaagggaatg
     3301 atcagatatg agataagcgc tgcaaccgca atataggcta cttcggtttt ataccagctg
     3361 cgtccgtgat gaaaacgaaa gcggactcgg taatcgccgt atacaaagcg cgaaactcca
     3421 tacggtatag ccagggccag aagcagcgct gcccccatca cggccatatg cgaatagctt
     3481 acgtcggtgg taatcggggt aatacccagg atcgctagag caataacaat aagcaacatg
     3541 tccttggcaa atagcttgtc tgcgagcaat aggctgaacc cggccagcaa taatagactc
     3601 cagcccatcg gcttaagttg caaaccaatc agcaccaccg ctgaagttag caaaagacca
     3661 acgcttatgt atcgagctat attcagcggg tgtacctaat tacgaattac tgtttcgagc
     3721 gggtaaatac taggtaatgt tagtgtcggc cggaacctcg gcgatgatga tctatacctt
     3781 catcgtcact accatcttcg ttgtcattga agtcgttgtt atcgttaaaa tcatgatcca
     3841 ttgcatcatt attatcgtcc gagtttacgt tccccggccc gctatttgaa ttggtagtat
     3901 ttgaagcgtc tccccccgtg gtattggttt cggttgctgg ctcattacgc tcgatagctc
     3961 cagtggatgc ccttacatca actcgggcac catctacaaa cctaacgctg tagactagca
     4021 caccatcttc cgtctcaagc tcaacctttc tgattgtact ccccggatgc tgagccattg
     4081 ctatgctttt cgcactagtt aaagtaatgg ttggtgtttg agcggttgtc cggtttgcgt
     4141 catcgcctcc atggcggaca atactagccg aaaccgtctt ttttgcagtt tgctgttgga
     4201 ccgctacgaa cgtacccgct ccaagcaata tgaccgccac aggcaagatg agcggaagat
     4261 atgcaatgcc acgctgattc ttcaaattca taactcaagc ctcccccatt tattgaaaag
     4321 tagcaccagt atacagaatg tattgtgcaa atgcactatg caaattgcta agtaggttag
     4381 cgttcccaga atttgcgtat tatatcaaga gccaaggtgg aaaggagaaa tccggcaacc
     4441 ggcactaccg ccccc
//
LOCUS       DASVGB010000051         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_175125, whole genome shotgun sequence.
ACCESSION   DASVGB010000051 DASVGB010000000
VERSION     DASVGB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175125"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VF272_01575"
     CDS             <1..1698
                     /locus_tag="VF272_01575"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017577290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX7259602.1"
                     /translation="SEFEKKYIEYEQKADRGEIKTFKRMPAKELWRKMITMLFETGHP
                     WITFKDPSNVRSPQDHVGVVHNSNLCTEITLNTSAEETAVCNLGSVNLGRHMTNGKLD
                     EAKIAKTVKTAMRMLDNVIDLNYYPTAEAKNSNMRHRPVGLGLMGFQDALYLADMDFD
                     SDETVRFADYSMELISYHAILTSAELAAERGAYSSFEGSKWDRDIFPIDTLNLLEQER
                     GMPIGVMRTSKLDWTPVRAAVKKHGMRNSNTMAIAPTATISNISGCFPCIEPIYKNLY
                     VKANISGDFTVVNTYLVEDLKKLGLWNQTVLEEIKQHDGSIGDVSVIPPRLRSKYKEV
                     FDIDTEWLIKAAAYRGKWIDQSQSLNIFYNGTSGRRLAELYMLAWHMGLKTTYYLRTQ
                     AASTIEKSSVALQQSQAAPMATAAQLVAPVPAAMTSGAAPSAPIVAPPAVPVAPIAAP
                     VSTPPMTSPAPVAPSTPSSSPFASAPATPVSSQVPPVGAEMSMAVEPVSPSFAPSMSS
                     GIEPIGFTAPTSTLVAAAQPSAPTVTIARPEGQVTILSSKPAPKLCLIEDPDCEACQ"
     gene            1711..2769
                     /locus_tag="VF272_01580"
     CDS             1711..2769
                     /locus_tag="VF272_01580"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX7259603.1"
                     /translation="MEESIKPQAAAPSSPQASAIQRKTLLNASGTDPNKILPIKYNWA
                     RAHYKKGVANNWVPEEVPMQDDIELWKRAGGLSDAERRLILWNMGFFSTAESLTANNI
                     VLSVYKHLTNPECRQYLLRQAYEEAIHTDTFIYCCDSLGLNPDEVYTMYLTIPNIAAK
                     DDFVVDMTKSIFNDSFKPDSPENIQRYVHDLIGYYVIMEGIFFYAGFVMMLSFKRQNR
                     MVGVGTQFEYILRDESVHLAFGSDLINGLVDENPTIWTADFQKEITDNVVKAVELEHA
                     YAKDCLPEPLLGLNEKTIRQYLEYIADRRLERIRLAKVYGTPSPFPWMSEITDLKKEK
                     NFFETTVTEYQTGGNLDW"
     gene            2780..3031
                     /locus_tag="VF272_01585"
     CDS             2780..3031
                     /locus_tag="VF272_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7259604.1"
                     /translation="MPQVTIFSTTTCKYCKEVKEFLATHNQPYEEILLDLRPEFIQES
                     VSLSGSRGVPVTKVVRDDGSTVGVLGWDQPALEEALQLA"
BASE COUNT          877 a          783 c          803 g          798 t
ORIGIN      
        1 agcgagttcg aaaagaagta tattgaatat gagcaaaaag ccgatcgcgg tgaaatcaag
       61 acatttaagc gtatgccggc taaagagttg tggcgcaaga tgattacgat gttgttcgaa
      121 accggtcatc cttggattac atttaaagat ccctcaaatg ttcgctcacc ccaggatcac
      181 gtcggcgtgg tacacaactc caacttgtgt actgaaatta cactcaacac ttcggccgaa
      241 gaaacagcgg tgtgcaacct tggttcggta aacttgggtc gccacatgac gaatggcaag
      301 cttgatgaag ctaagatcgc caaaaccgtt aaaacggcta tgcgcatgct cgataatgtt
      361 atcgacttaa actattaccc aaccgctgaa gccaaaaatt cgaacatgcg ccatcgtcca
      421 gtgggtctag gattaatggg cttccaggat gctttgtatc tagccgatat ggactttgat
      481 agcgatgaga ctgtccgctt tgccgactac agtatggagt taatttctta ccatgcaatt
      541 ttgacctcgg ccgaattggc agcagagcgc ggcgcctata gcagcttcga aggatctaag
      601 tgggacagag atatcttccc gattgacacc ctgaatcttc ttgagcagga gcgcggcatg
      661 ccgattggcg ttatgcgtac ctcaaagctc gactggacgc cggttcgtgc agcggttaaa
      721 aagcacggca tgcgcaactc taatactatg gcaattgcgc ctaccgctac catctccaat
      781 attagcggtt gctttccctg tatcgagcca atctacaaga atctctatgt gaaagctaat
      841 attagcggcg actttaccgt agtgaatacc tacttggttg aggatctcaa gaagcttggt
      901 ttgtggaatc agacggtact ggaagagata aagcagcatg acggcagtat tggcgatgta
      961 tcggtgattc cgccgcggtt acgttcgaaa tataaggaag tgtttgatat tgataccgaa
     1021 tggctgatta aggctgcagc ttatcgtggc aagtggattg atcagagcca gtctctcaat
     1081 atcttctata atggtacatc cggccggcgc ttggctgaac tctacatgtt ggcgtggcac
     1141 atgggtctta aaacgaccta ttatctccgc acccaagcgg ccagtacgat tgaaaagagc
     1201 tcggtagcgt tgcagcagtc tcaggcagcc cccatggcta ccgctgccca gcttgtagca
     1261 ccagtcccgg cagccatgac atcaggtgct gctccgtctg caccgattgt tgcgcctccc
     1321 gcggtacctg ttgctccgat tgccgctcca gtatcgacac ccccaatgac ctctcccgca
     1381 ccggtagcac caagtactcc ttcaagctcg ccatttgcat ctgcaccggc aacacctgta
     1441 tcatcacagg taccacccgt aggggcggag atgtcaatgg ctgtggaacc ggtctcaccc
     1501 tcattcgcgc ctagcatgag ttcagggata gaaccaatcg ggttcacagc accaaccagc
     1561 actttggtag cagcagccca gcccagcgcg cctactgtaa caattgctcg tccggagggg
     1621 caagtaacca ttctttcgag taagccggcc cccaagctgt gcttaattga agatcctgac
     1681 tgcgaagcct gccaataaga aagaggaacc atggaagaat caataaagcc gcaagcggca
     1741 gctccgtcat cgccgcaagc atctgcaata caaagaaaaa cgctgctgaa tgcttcaggc
     1801 accgatccaa acaagatttt gcccatcaaa tacaactggg cccgtgcgca ttataagaaa
     1861 ggtgttgcca ataactgggt gccggaagaa gttccaatgc aggatgatat tgaactgtgg
     1921 aaacgggcag gggggttaag cgatgccgag cggcgcttaa ttctctggaa catgggtttc
     1981 ttttcaaccg ccgagagtct tactgccaac aacattgttc tttcggtcta taagcaccta
     2041 acgaatccgg aatgccgcca gtatttgttg cgccaagctt acgaagaagc gattcatacc
     2101 gataccttta tttattgttg cgactcactc gggttgaatc cggacgaagt gtacaccatg
     2161 tatttgacta ttccgaatat tgctgccaag gatgattttg tggtcgacat gactaaaagt
     2221 attttcaatg atagcttcaa gcctgactcg ccagaaaata ttcagcgtta cgtccacgac
     2281 ttaattggct attacgtaat catggaaggc atcttcttct acgccggttt cgtaatgatg
     2341 ctgtcgttca agcgccagaa ccggatggtt ggcgtaggca cccagtttga atacatattg
     2401 cgcgatgagt cggtacactt ggcttttggt tcagacttaa ttaatggcct ggttgatgag
     2461 aaccccacga tttggacggc tgattttcaa aaagaaatca ccgacaacgt tgttaaagct
     2521 gttgagctgg agcacgcata tgccaaggac tgcctacctg agccgctact cggcttgaat
     2581 gaaaaaacga ttcgccagta tttggaatat atcgccgata gacgcctgga gcgcattcgt
     2641 ttagctaagg tctacggaac tccgagcccc tttccatgga tgagcgaaat aacagattta
     2701 aagaaagaga aaaacttttt tgaaacgacg gttactgagt atcagaccgg cggtaatcta
     2761 gattggtaaa ggagagacaa tgccacaagt aacaattttt tcgaccacga cctgcaagta
     2821 ctgcaaggaa gttaaagagt tcctagcaac ccataatcag ccatatgagg aaattctgct
     2881 ggatctgcgc ccggagttta ttcaagaatc ggtatcattg agcggctcgc gcggtgttcc
     2941 ggttactaaa gttgttcgtg acgacggcag tacggtaggc gtacttggct gggaccagcc
     3001 agcactagaa gaagcactgc aactagcgta aataactaga gcaagccata agcctcataa
     3061 tattgcccat tgattggcct ctataaggta ttaaaatccc ctctagatag tgaaagtata
     3121 gaacaaaaat gataggggga tcggctatga gttttcgtgt gcgactttcc ttggcctcac
     3181 tggcgtttac ggtaacatta ggactcatag gttttattgc cccggcagta gccgacaagg
     3241 gtggtaaggg agttggccaa t
//
LOCUS       DASVGB010000052         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_30454, whole genome shotgun sequence.
ACCESSION   DASVGB010000052 DASVGB010000000
VERSION     DASVGB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30454"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VF272_01590"
     CDS             complement(<1..593)
                     /locus_tag="VF272_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7259605.1"
                     /translation="MKSVEEIQKVANRIRLDIIEMLVEAGSGHSGGPLGMADIFATLY
                     FQVLNHDPQNPDWPERDRLILSNGHIVPVRYATMARAGYFPVDELMTLRKFGSRLQGH
                     PERVKLLGLETTSGPLGSGLSAASGMALVLKMEKKKSQRVYCLVSDGEFDEGNIWEAV
                     LFAGNYKLSQLTMIMDRNNIQIDGSTEEVMPLGDVAE"
     gene            complement(590..1228)
                     /locus_tag="VF272_01595"
     CDS             complement(590..1228)
                     /locus_tag="VF272_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259606.1"
                     /translation="MAIVVPTVLASTPAEYASMLATAESISKRIHVDVCDGQFAENKT
                     VGMAHVYSSEKTEVDLHLMLKDPASELETALSLKPALIIFHAESDGNLERMIDQVRTF
                     GIKAGLAVLSTTSIESARQLLGKVDHLLVFTGTLGQNGGEFQADQLSRAQEARAINPG
                     LEVSVDGGVSGSNAALIVREGVDVLYSGGFLQNSPDPKAAYESIMAAAGAAA"
     gene            complement(1229..1681)
                     /locus_tag="VF272_01600"
     CDS             complement(1229..1681)
                     /locus_tag="VF272_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX7259607.1"
                     /translation="MKPAVFIGADHGGFEYKNQIREHLIHQGYQVEDVGAETLDPADD
                     YPQFAYAVATKILGEEGSVFGILVCRGGQGMAMAANRVAGIRAAVAWSPEVAQKTRED
                     NNANVLCLPGDMIDIDTSLDIVDTFIKEPFSNEERHVRRLGQIEDLFG"
     gene            complement(1678..2442)
                     /locus_tag="VF272_01605"
     CDS             complement(1678..2442)
                     /locus_tag="VF272_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7259608.1"
                     /translation="MLPWKDLDVDVVIESTGFFVKREDAGKHLTAGAKRVVLSAPAKG
                     DDVPTYVIGVNDGDISAEDTIVSNASCTTNCITPVMAVLEATVGVEKALMTTVHSYTA
                     SQRLQDAPAKDLREARNAAENIVPTTTGASKAAARALPALKGIFDGLSIRVPTPVVSL
                     SDITFVAKKATTIEEINEAMVKASHEANFKGVLAATDEELVSRDYVGNSHSAIVDLKL
                     TNVVSGNLVKVVAWYDNEWGYSNRLVEMVMKVGKTI"
BASE COUNT          595 a          695 c          545 g          634 t
ORIGIN      
        1 ttttctgcta catctcccag tggcataact tcttcggttg aaccgtcgat ttgaatgttg
       61 ttgcggtcca ttatcatggt tagctgactc aacttatagt taccggcgaa caatacggcc
      121 tcccagatat tgccttcatc aaactcgccg tcgctcacta agcaataaac ccgttgcgat
      181 ttcttttttt ccattttcaa aactaaggcc ataccgctgg cggcacttaa ccccgaccct
      241 aatggaccgc tggtggtttc aagaccaagt agcttaaccc gttccggatg cccttgcagg
      301 cgagaaccga atttacgcaa cgtcataagc tcatctactg ggaaatagcc ggctcgggcc
      361 atagttgcgt aacgtaccgg tacgatgtgg ccattggaaa gaattagtcg gtctcgctct
      421 ggccagtccg gattttgtgg atcatggttc agcacctgaa aatacagcgt ggcaaagatg
      481 tctgccattc ctagcgggcc gccggaatgc ccgctgccgg cttccaccag catttcaata
      541 atatctaggc ggatgcggtt cgcaactttt tgaatttctt caacgctttt catgcagcgg
      601 ctcctgctgc tgccataatt gattcatagg cggctttagg atcaggcgag ttttgcaaaa
      661 aacctccgga atagagaaca tctacgcctt cccgcacgat cagagcagcg ttgctaccgg
      721 aaactccccc atccacgctt acttctagac cggggttaat agcccgcgcc tcctgggcac
      781 gactcaattg gtctgcctgg aactccccgc cattttgccc tagtgtcccg gtaaaaacta
      841 gcagatggtc aaccttccca agcaactggc gagccgattc tatcgatgtt gtcgacagga
      901 ccgccaagcc ggccttgatg ccgaacgtac gaacctggtc aatcatcctc tccaaattac
      961 catcggattc ggcatggaaa ataattaagg cgggcttcaa acttagggct gtttccagtt
     1021 cggaagcggg atccttaagc atcagatgca ggtccacctc tgtcttctcg ctactataaa
     1081 catgtgccat gccgacggtt ttattctccg caaactgtcc gtcgcagaca tcaacatgaa
     1141 tacgcttact aatactttca gcggtagcga gcatcgaagc atactctgct ggcgtacttg
     1201 ccaatacggt tggaacaacg attgccattt aaccgaatag gtcctctatt tgacctagtc
     1261 gccgaacatg gcgctcttcg tttgaaaatg gctctttaat aaacgtatcg acaatatcca
     1321 aagatgtatc aatatcgatc atatcgccgg gcaagcataa aacattggca ttgttgtcct
     1381 cgcgagtctt ctgagccact tccggcgacc atgccaccgc cgcccgaatg ccggctacgc
     1441 ggttagcggc catggccata ccctggccgc ctcgacaaac caatatgcca aacaccgagc
     1501 cttcttcacc gaggattttg gtagcaacgg cataggcaaa ctgcggataa tcatctgccg
     1561 gatcaagtgt ttccgccccg acatcttcta cctgatatcc ctggtgtatc aaatgctcgc
     1621 gaatttgatt cttgtactca aatcccccat gatcggcgcc aatgaacacg gctggcttca
     1681 tatggtcttg cctactttca tcaccatttc aaccagccga tttgaataac cccattcatt
     1741 gtcataccaa gctaccacct ttaccagatt ccccgaaaca acattggtca gcttaaggtc
     1801 aacaattgcc gaatggctgt tgcccacata atcgcgcgat accaactctt cgtccgtagc
     1861 ggccaaaacg cccttaaagt tggcttcatg gctggccttc accatagcct catttatttc
     1921 ttcaatcgtg gtagcttttt tggcaacgaa ggtaatgtcg ctcaagctca ccactggggt
     1981 gggaacccga atgcttaagc cgtcaaaaat gccctttaat gccggtaatg cccgagctgc
     2041 agctttggat gcgccggtcg tagtcggcac aatgttttca gcggcgttgc gcgcttcacg
     2101 caaatctttt gccggagcat cttgcaggcg ttggctggca gtataagaat gaacagtggt
     2161 catgagggct ttttcaaccc caaccgttgc ctccaatact gccatcacag gtgttatgca
     2221 gtttgtagta caggaagcat tggagacaat cgtatcttcg gctgaaatat ccccgtcatt
     2281 tacgccaatc acataggttg gtacatcgtc tcccttggcc ggcgctgaca gcaccacacg
     2341 cttggcaccg gcagttaaat gtttgccagc gtcttcgcgc tttacaaaaa agccggtact
     2401 ttcaatgacg acgtcaacat caagatcttt ccaaggcaac aaagctggat ccttttcggc
     2461 atacactca
//
LOCUS       DASVGB010000053         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1271444, whole genome shotgun sequence.
ACCESSION   DASVGB010000053 DASVGB010000000
VERSION     DASVGB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271444"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VF272_01610"
     CDS             complement(<1..762)
                     /locus_tag="VF272_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7259609.1"
                     /translation="MAKPKVAIVHDWLTNQGGGERVVWALHQAYPDAPIYTSVFNAEA
                     LPQFAGLDVRTSFLQRWPLAQTKHQLFPWLRTLAFEAFDFSGYDVVISSSSAEAKGII
                     TKPETLHISYCHTPTRYYWSDYASYRKNTGFGVLSPLVRLVMPGMVKRMRIWDFAAAQ
                     RVDAFIANSKYVCQRIAKYYRRDSTVINPPIDTQRFKVHHGPRSGFVVVSRLIPYKRI
                     DLAVQACSQLNLPLTVIGDGSELAGFKEMAGPSIRF"
     gene            complement(763..2220)
                     /locus_tag="VF272_01615"
     CDS             complement(763..2220)
                     /locus_tag="VF272_01615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7259610.1"
                     /translation="MRKRSELFFSLLLLPIDFIAILSAFIAAYTWRVFLDDRPVAYPL
                     GIEFFLQIFLLVIPVWILFFAINGLYNLSSQRGRLWELKAIFVAVSSGVMFMIVLDFI
                     SKTPIFPSKAVPVYAYGLSLITVVVGRLLVRAVQRSLFRKGIGLYRVVIIGSGVLAQQ
                     LADALKLADRSGYEIVGVVDTAKGAQKRMKPFRVDLSLTNLLNRLDGAKVDEFIQADS
                     SLEPDEVFEIVEYAATNHITYRFVPNQFGLFATHSELGTLAGMPMVAMKQTPLDGWGR
                     IVKRLFDVVAAVAGLIILSPLFLLIAILIKLTDSGPVFYSHTRLSRVGKSIRIHKFRS
                     MRQLFNTGDTYKGKSEVEIFTALDRPDLVEEFARNQKVKDDPRVSRIGAFLRRTSLDE
                     LPQLFNILKGDISLVGPRPIVAQELERYGTGRPTLLSLKPGLTGLWQVSGRSDVSYDE
                     RVKLDIYYIENWSLWLDIKIIARTIVIVLTRQGAH"
     gene            complement(2284..2686)
                     /gene="rnpB"
                     /locus_tag="VF272_01620"
     ncRNA           complement(2284..2686)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF272_01620"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2905..>3607)
                     /locus_tag="VF272_01625"
     CDS             complement(2905..>3607)
                     /locus_tag="VF272_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7259611.1"
                     /translation="KSRKVLHNVSFRLEAGQTLALVGPSGTGKTTITKLMLRFYEPTE
                     GTILINDESIEHFSGESIRQHIGMVMQDVALFNDSVEQNLQLANPRATKVHVRAAAEQ
                     AHADQFIEKLPEKYNTLVGERGIKLSGGEKQRVAIARAILKQPDLIILDEATSALDSE
                     SERYVQAGLSDLMKDRTAVVIAHRLSTVMRADQILVLRGGKIVERGIHEDLAVKPGGL
                     YAKLFKLQTEGFIKI"
BASE COUNT          904 a          983 c          891 g          829 t
ORIGIN      
        1 aaagcggatc gatgggccgg ccatttcctt aaaaccagct agctcgctgc cgtcaccgat
       61 aaccgtcagg gggaggttga gctgactgca tgcctgcacc gccaaatcga tccgcttata
      121 gggaatcagc cgtgaaacta ccacaaatcc gctgcgtggc ccatgatgca ccttgaagcg
      181 ctgggtatca atcggcgggt taataacggt tgagtcgcgc cggtagtatt ttgcaatccg
      241 ctggcagacg tatttggaat tagcaatgaa tgcatccacc cgttgggcgg cggcaaagtc
      301 ccagattcgc atgcgcttta ccatgccggg cattaccagc cgcaccagag gtgaaagcac
      361 gccgaacccg gtattcttac gatacgaggc gtaatcgctc cagtagtagc gggttggggt
      421 gtggcagtat gagatgtgta gagtttccgg cttggtaata attcctttgg cttcagcact
      481 ggaagacgaa atcacgacat cgtagccgct aaaatcaaaa gcctcgaaag ctaaggtgcg
      541 caaccacggg aaaagttggt gtttggtttg agccaacggc cagcgctgca aaaaggaggt
      601 gcgtacgtct agcccggcaa actgcggtaa agcctctgca ttaaaaaccg aggtataaat
      661 tggcgcgtcc ggataggctt ggtgcagcgc ccaaaccacc cgctcgcctc ccccttggtt
      721 ggtcagccaa tcgtgaacaa tggcaacctt gggcttagcc atttaatggg ccccttgacg
      781 ggtcagcacg atcacaatgg tgcgcgcaat gatcttaata tccaaccaga gactccagtt
      841 ttcgatgtag taaatatcca gcttcacccg ctcgtcataa ctgacgtcac tgcgccccga
      901 aacctgccag aggccggtaa ggccgggctt gagcgataat aaggttggcc gaccggtgcc
      961 gtaacgttct agctcctgag ctacaatcgg acgcggaccc accaggctaa tatcgccctt
     1021 taagatatta aatagctgag gcagttcgtc gagactggtc cggcgcagga aagcccctat
     1081 ccggctcacc cgcggatcgt cttttacttt ttgattgcgc gcaaactctt caaccagatc
     1141 aggccggtca agagcggtga aaatttccac ctcagactta cctttatacg tatctccggt
     1201 gttaaagagc tgtctcattg aacgaaattt atggatacga atcgacttcc cgacgcggct
     1261 caatcgggta tggctataaa acaccgggcc cgaatcagtg agtttaataa gaatggcaat
     1321 gagcaagaat aagggggaca aaataatcag acccgccact gccgcaacca cgtcaaaaag
     1381 ccgctttact atcctccccc atccgtctaa cggtgtctgc ttcattgcca ccatcggcat
     1441 gccggcaagc gtgccgagtt cggagtgcgt cgcaaacagc ccaaactgat tcggcacaaa
     1501 ccggtaggta atatggttcg tagcggcata ttcgacaatc tcaaaaacct catccggctc
     1561 cagcgacgaa tcagcttgaa taaattcatc aaccttagcc ccatcaaggc gatttaaaag
     1621 attggtgagc gacagatcga cccggaacgg tttcatgcgt ttttgggcgc ctttggcggt
     1681 atcaaccacc cccacgatct cgtatccgga acggtcagcc aacttaagcg catcggccag
     1741 ctgctgtgct agcaccccgc tgccaataat tactacccgg tataagccaa tgcctttacg
     1801 gaacaggctg cgctgcaccg cccgcactag cagacgcccg acgaccacgg taatcaaact
     1861 cagaccgtag gcgtacactg gaaccgcctt cgaagggaaa atcggtgttt tgctaatgaa
     1921 gtccagaaca atcataaaca ttacacctga tgagacggcc acgaagatgg ctttaagctc
     1981 ccatagccgg ccgcgctggc tggaaaggtt atagagaccg ttaatcgcga agaataatat
     2041 ccaaaccgga ataaccaata ggaatatctg caggaagaat tcaatgccca gcggatacgc
     2101 taccggccga tcatcaagaa aaacccgcca ggtataggcg gcgataaagg cagacagtat
     2161 cgcaataaaa tcgatgggca gcagtaaaag actgaaaaat agctcgcttc gtttacgcat
     2221 gggttatatc tgtcattacg gtaagcattt tacaccaaaa aagcctccgt taggaggtct
     2281 ttttggttaa ggttgcctgt aagccggatt ctgtcccccg cgatgctcgc gggtgtatgg
     2341 ccatctatct tgccataatg ttgccatcat ggtcatgcgg ctcgtacgct gattggagcg
     2401 agtactcccc tgtttccagg taaatcagca gccttgcagc ggacggggtt tacctccctc
     2461 ccatgtcacc atgagaggct gtgggctctt accccactcg tttcaccctt acctcagcaa
     2521 gctgaggcgg tctcgtttct gtggcacttt ccctaggctt gagcgacgct gttttctcga
     2581 gagaaacacc ggcgcttact cccggttgcc gttaacaacc gtccttactc tatgctgtcc
     2641 ggactttcct ctggtggttt aagccagcgg ccatccggca accttaagtc ccgttatcat
     2701 agcaaaaacg aggctaaagc tcaagcaata agacgctatt tgcgggtgta tgacttgagg
     2761 acttacttca ttgagcccgt ttagtgtccc aaggatacta gtttcggatt aattttcagt
     2821 taaaaacgcg ccggaatagt atccttggaa tactattctg caaaaacact agtatggcta
     2881 caattaagtg ccagtgccaa gcagctagat cttaatgaac ccctcagttt gcagtttgaa
     2941 gagcttagca tacagcccgc ccggcttaac tgccaaatcc tcatggatgc cgcgttcaac
     3001 aattttaccg ccgcgcagta ccaggatctg atcggcacgc ataacggtgc tcaggcgatg
     3061 agcaatgact acggcggtac ggtctttcat aaggtcggat aaaccggcct gcacatagcg
     3121 ctcgctttca gaatccagcg cactggtagc ttcatccaga atgatcaaat cgggctgctt
     3181 taaaatggcg cgggcaatcg ccacacgctg cttttcaccc ccgctcaact taatcccccg
     3241 ctcgcctacc agggtgttgt acttttccgg caatttctct atgaattgat cggcatgggc
     3301 ttgctcggcg gcggcccgca cgtgcacttt ggtagcgcgg gggttagcga gctgcaagtt
     3361 ttgctccaca ctgtcattaa acagtgccac atcttgcatc accatgccga tatgctgccg
     3421 tatcgactca ccgctaaagt gctcaatcga ttcgtcatta atcagaatgg tgccttcggt
     3481 cggctcatag aaccgcagca ttaacttggt aatcgtagtc ttgccggtac cgctagggcc
     3541 tacgagagcg agcgtttgac cggcctcaag cctaaaactg acattatgca gcaccttgcg
     3601 gctttta
//
LOCUS       DASVGB010000054         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_887464, whole genome shotgun sequence.
ACCESSION   DASVGB010000054 DASVGB010000000
VERSION     DASVGB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887464"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VF272_01630"
     CDS             <1..254
                     /locus_tag="VF272_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX7259612.1"
                     /translation="KVKSGPSEDDWYRLRICESGNNYANKHNSLYRGAYQFSYATWKT
                     VGGSGDPADAAPAEQDMRAVMLFNRSGWSQWPVCGRYLR"
     gene            complement(264..527)
                     /locus_tag="VF272_01635"
     CDS             complement(264..527)
                     /locus_tag="VF272_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7259613.1"
                     /translation="MSIVAWIVFGGLAGWIASIIMRTDQEQGAVGNVIVGIIGAILGG
                     YLMQALGASGTTGFNFYSLIVAILGAVVLLAIVNMFRRGSMRT"
     gene            699..1529
                     /gene="rsmA"
                     /locus_tag="VF272_01640"
     CDS             699..1529
                     /gene="rsmA"
                     /locus_tag="VF272_01640"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7259614.1"
                     /translation="MDLSNIDELRGALQLAGLRPRKGLGQHFLVNRQSLEAVMAAGKL
                     TPNDTVVEIGPGLGVMTAPLAERVKQVVAVETDGVLVALLRRHLPKNVEIIQADIMRF
                     NLNQLPAGYKVVANIPYYLTSAITRLLTEAENRPTVVSLLMQKEVAERITAQPGKMSI
                     LALSVQYYGQPRLLETVERHKFWPPPKVDSAVLQIEMYDKPLFEADRSKLFHLIKAGF
                     GEKRKMLKTSLAGGLNIEVSLSIELLWLASLDLTARAQELALDDWRRLYNAAIKRLVL
                     "
     gene            1587..3023
                     /gene="metG"
                     /locus_tag="VF272_01645"
     CDS             1587..3023
                     /gene="metG"
                     /locus_tag="VF272_01645"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7259615.1"
                     /translation="MSKFYLTTPIYYVNDLPHIGTAYTTIAADVLARYHRQQNDEVLF
                     ATGSDENGQKIVQAAEKAHQTPAEYVDTMAKSWQQTWDQLGISHDRFIRTTDKDHIAG
                     VRAFIAAVQASDDMYKGVYEGLYCVGCEEFKKESDLVNGHCPEHPNQELEHIKEDNYF
                     FRLSKYQDQLLQHIEANPNFVQPESRRNEVIQFIKHGLEDFSVSRPNKGWGIPWPDDK
                     DQVVYVWFDALVNYLVVAGYPNDASKWWPADLHLVGKNIIKFHCVYWQAMLMSAGLPL
                     PQRVFAHGFFTVDGQKISKSLGNAINPVELAGTYGVDALRFYLLREITFGGDGEFSHE
                     RFRQVYQSELADDLGNAVQRVASMIGRYLGGTISEVPKSGHDVGLVHQAMAELRLDKA
                     LDELWVHVRSINQYIEQEKPWELAKTDPNQLTLVLQQSVADLLHVAELLLPFMPATAQ
                     RIAQTFAGGQVHPEVGILFPKFDEAPAK"
     gene            3036..3788
                     /locus_tag="VF272_01650"
     CDS             3036..3788
                     /locus_tag="VF272_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7259616.1"
                     /translation="MIDTHAHIHFDSFAGQVDAMLHRAFEAGVEQMITVGVTTADSRK
                     AVDLASTYENVWAAVGIHPHDAGEASQGIDYLRDLAGRRKVVAIGECGLDQVKSQTTA
                     EEQEKALRLQIELAQERKLPLIFHIREAFEPFWAIIDDYPDVRGVVHSFSATRKEAEQ
                     VIERDMLLGINGIVSFPKSQDLGEAVKYLPSTHLLLETDCPFLSPVPFRGKTNEPARV
                     TNIAEKIAELRGETLATVAAYSTANAKRLFSL"
BASE COUNT          947 a          894 c         1052 g          957 t
ORIGIN      
        1 ccaaagttaa aagcggacca agcgaagacg attggtatcg gttgcggatc tgtgaatcgg
       61 gaaataatta cgctaataag cacaacagct tgtaccgtgg tgcttatcag ttttcttacg
      121 ctacatggaa aacggtgggc ggtagcggtg atccggctga cgccgcgcca gctgagcagg
      181 acatgcgggc agtgatgctc tttaatcgca gtggctggtc gcagtggccg gtgtgcggcc
      241 ggtatctccg ctaataaccg gtgttaggtg cgcatgcttc cgcgacggaa catgttgacg
      301 attgccagta acacgacggc gcctaagata gcgactatca ggctgtagaa attaaagccg
      361 gttgtaccgc tggcaccgag ggcttgcata aggtagcctc ccaggatagc gccaataatc
      421 cctacgatca cgttaccgac agcaccctgt tcttggtcgg ttcgcataat gatggaagcg
      481 atccaccctg ctagcccgcc aaatacaatc cacgctacta tactcaacaa atccattaga
      541 ccctcccttc acttaactta tttactataa cctttagatt acctgagcgc tccaattgct
      601 tcatgggtaa aattactaat tattggccgg tatacgggca gtaaatccgc ttttttgtat
      661 aatccggggt ttcgtatcag gtaggttaga atagaggcat ggatttaagc aatatagatg
      721 agttgcgtgg agcattacag ctagcgggtt tgcgcccgcg caagggcctg gggcagcatt
      781 ttctggtgaa ccggcaaagc ctggaagcgg ttatggcggc cggcaagctt acaccgaacg
      841 atactgttgt agagattggg ccgggattag gcgtgatgac cgctcccctt gccgaacggg
      901 ttaaacaggt tgtggcggtg gaaactgatg gggtgctggt agcactattg cggcgccatc
      961 ttccgaaaaa tgtagagatt atccaagccg atattatgcg gttcaattta aatcagttgc
     1021 cggcaggcta taaggtagtg gccaatattc cttactatct aacttcagca attacccggc
     1081 tgttaaccga ggccgaaaat cggccaacgg tggtctcctt gctgatgcaa aaagaggtag
     1141 ccgagcggat aacggcgcaa cccgggaaga tgagtatcct agccctaagc gttcaatatt
     1201 acgggcagcc gcgtttgctt gaaacggttg agcgccacaa gttctggccg cctccaaagg
     1261 ttgattcggc ggtgctgcag attgaaatgt acgacaagcc gctgttcgaa gccgaccgct
     1321 caaaactttt ccatctcata aaagctggtt tcggagaaaa gcgtaaaatg ttaaaaacca
     1381 gcttggccgg cggcttgaat attgaggttt cattatctat tgaattgctt tggctggcct
     1441 cgctggattt gaccgcccgc gcccaggagc ttgccttgga tgattggagg cggctgtaca
     1501 atgcggcgat taagcggttg gtgctgtagg cccttcttaa gataaccggc atagacggtc
     1561 aacctgattt tcgctacact agccctatgt caaaatttta ccttacgact cccatttatt
     1621 atgtgaacga cttgccacac attggcacgg cttacacgac gattgcggct gatgtgcttg
     1681 cccgatacca tcgtcagcaa aatgatgagg ttttgttcgc aaccggttcg gatgaaaatg
     1741 gccaaaagat tgttcaggcc gccgaaaagg ctcatcaaac cccggctgaa tatgtcgata
     1801 caatggccaa atcatggcag caaacctggg atcaactcgg cattagccac gatcgattca
     1861 ttcggacaac cgataaagac catattgccg gcgtccgcgc atttattgct gcggttcaag
     1921 cctcggacga tatgtacaaa ggcgtctatg aaggcctgta ttgcgtaggc tgcgaggaat
     1981 ttaaaaaaga gtccgatttg gtaaatggcc actgtccaga gcatcccaat caagaattag
     2041 agcacatcaa ggaggataat tatttcttta gactcagcaa gtaccaagac caactgctgc
     2101 agcatattga ggcgaatccg aattttgtgc agccggaaag ccgccgcaac gaggtaatac
     2161 agtttattaa gcatggccta gaagatttta gtgtctcgcg gcccaacaag gggtggggta
     2221 ttccctggcc ggacgacaag gaccaggtgg tttacgtttg gtttgatgcc ttggttaact
     2281 acttggtggt agccggctat ccgaatgatg ctagcaaatg gtggccggca gatcttcatt
     2341 tagtgggcaa gaacattatt aagttccatt gtgtgtactg gcaagccatg ctcatgagtg
     2401 ccggtttgcc gcttcctcag cgagtgtttg ctcacggctt ctttacggtt gacggccaga
     2461 aaatttcaaa atccctcggt aatgccatta atccggttga gctggccggc acctacgggg
     2521 tagatgcttt gcgcttctat ttgttgcgtg aaattacttt tggcggcgac ggcgagttca
     2581 gccatgagcg ttttcgccaa gtgtatcaat ccgaactggc cgatgatttg ggaaacgctg
     2641 tgcagcgggt agcttcgatg attggacgct acctgggtgg tactataagc gaggtgccaa
     2701 aatcagggca tgacgtaggt ttggtgcatc aggctatggc agagctgcgc ctcgataaag
     2761 ccctggatga gctttgggtg catgttcgca gcatcaacca gtatattgag caggaaaagc
     2821 cgtgggagct ggcaaaaacc gatcccaacc agctaactct ggtattacag caatcagttg
     2881 ccgatctttt gcatgttgcc gagctactct taccctttat gccggcgaca gctcagcgga
     2941 ttgcccagac atttgcaggg gggcaggtgc atccggaagt cggtatttta ttccccaaat
     3001 ttgatgaggc tcccgccaag taggtccttt tggttttgat tgatacccat gctcacattc
     3061 attttgatag ttttgccgga caggtagatg caatgcttca ccgtgctttt gaggctgggg
     3121 tagaacagat gattaccgtc ggagtgacca cagctgatag tcgcaaggcg gtggacttgg
     3181 cctctaccta tgaaaatgtt tgggcagcag ttggcattca cccccatgat gcgggagaag
     3241 cttcccaggg cattgattat ttgcgggact tggctggacg gcggaaggta gtggcgattg
     3301 gcgagtgtgg gctggaccag gttaagtcgc aaaccactgc cgaggagcaa gagaaggcct
     3361 tgcgcttaca gattgagtta gcgcaggagc gtaaactgcc gttaattttt catatccgcg
     3421 aggcatttga gcctttctgg gcaattatcg atgactatcc ggacgtaaga ggggtggttc
     3481 atagctttag cgccacacgg aaagaggccg aacaggtgat cgagcgggat atgctcttgg
     3541 gcattaacgg cattgttagc tttcccaaaa gccaggatct aggcgaggcg gtcaaatatc
     3601 tgccaagtac acatttactc ttggagacgg attgcccttt cttaagtcca gtaccatttc
     3661 gcggtaaaac taatgaaccg gccagagtaa ccaacattgc cgaaaaaata gcggaactgc
     3721 gcggcgaaac gctggctacc gtggcagcgt actcgacggc gaatgccaag cggttattca
     3781 gcctatgaat acgtatggat atttggatta tgctgctgct acgccgattg atcctcgggt
     3841 tacagaggtt
//
LOCUS       DASVGB010000055         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_58422, whole genome shotgun sequence.
ACCESSION   DASVGB010000055 DASVGB010000000
VERSION     DASVGB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58422"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="VF272_01655"
     CDS             <1..1879
                     /locus_tag="VF272_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX7259617.1"
                     /translation="PTGGTMFGTESIKAAYGTGKGSIVVRAVATIEEDKKGNQQIVVT
                     EIPYQVNKATLVDKIAELAKDKRITGISDLRDESNRNGIRIVVELKRDSYPKKILNQL
                     YRLTPMQGAFHVNMLALTDKGRQPRVMTLEMMLQEFIQHRQNVVRRRTEFELGKAKDR
                     AHILEGLKIALDHIDEVIKTIRASRTTEIAESNLIKQFKLTVIQAKAILAMQLRRLTG
                     LERKEIEDELAELRKLIAALEKILGDEKEILRIIKEELTELKEKHDNPRRTQIVASEL
                     GKFSEEELVPNEQVIITLTQGNYIKRIPANTYKSQGRGGKGIMGMTTKEEDVVEHLVL
                     THNHDFILFFTNKGRVFRQKVYEIPAASRTAKGQAIINLLQLGPDEVITSLNTFNTAS
                     AGKYLFMATKNGTVKKTALADYANVRANGLIAIKLDEGDELRWVKQTSGTDEIIMSTA
                     LAQAIRFKESDARPMGRATRGVRGIRLRPGDQVVGVDLVQPGSKLLVIMENGYGKRTS
                     VDQFSTHARGGVGIKAGVVTAKTGKALDVRALENDDEDLVIISSSGQVIRLPIKTVSL
                     IGRATQGVRIMRLKDSDKVASIALIGESKLELEIGVDGEFESSDEAEAETDARPASPP
                     A"
     gene            2130..>2944
                     /locus_tag="VF272_01660"
     CDS             2130..>2944
                     /locus_tag="VF272_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259618.1"
                     /translation="MEPERMPQRLLLKLGVIFLAGAMGSGAALASEEGSPAATTSPGV
                     SAEQQSAQDVVITTSDDAAITVSSTTSQSQSVVGSSQSSTDAGSAAAVSAGAGLEATA
                     SDLEAKSAVLNDDTLSGTPVHGASSLSGGFTSAGAEKPSVQAAAGAKAENQVRVALAW
                     AAYQMVTRNVAALQPIAPIELTPLSTGSNLPALPQNPTGWLGDLRVLLTSSLLPLLGE
                     LVSQPGSMASAVTLLSLKLILLILLVIAASLLLLDFYTARLRRSGYTDAARSDA"
BASE COUNT          770 a          683 c          778 g          713 t
ORIGIN      
        1 cccaaccgga ggaaccatgt ttggcaccga gtcgattaaa gccgcctacg gtaccgggaa
       61 gggctcaatt gtggtacggg cggttgccac tattgaggag gacaagaagg gcaatcagca
      121 gattgtcgtt actgaaattc cctatcaggt aaataaggcc accttagtcg ataaaattgc
      181 tgagttggct aaagataagc gtatcaccgg aattagcgat ttacgcgatg aaagcaaccg
      241 taatggtatt cggattgtgg ttgagctcaa gcgcgacagt tatcctaaga aaatcttgaa
      301 ccagctctac cgtctcacgc cgatgcaggg ggcattccac gtaaatatgc tggctctaac
      361 cgataaaggc cgccagcctc gcgtaatgac gctggaaatg atgctgcagg aatttattca
      421 gcaccgtcag aacgtggtgc ggcgtcgtac agagtttgag cttggtaaag ctaaagaccg
      481 ggctcacatt cttgaaggtc tgaagattgc gcttgatcat attgacgagg taattaaaac
      541 gattcgagcc agccggacaa ctgaaatcgc tgaatccaac ttgataaagc agtttaagct
      601 aacggttatt caggcgaagg ctattttagc gatgcagctg cgccgcctca ccggtttgga
      661 gcgcaaagag attgaggatg agctggccga gctacggaag ctgattgctg cgctagaaaa
      721 aattctaggc gatgaaaaag aaattctgcg gatcattaaa gaggaattga ccgaactcaa
      781 agaaaagcat gataatccgc gccgtaccca gatcgtggct tctgaactag gtaaattcag
      841 cgaagaggag ctggtgccca atgagcaggt tattatcacc ctgacccagg ggaactacat
      901 caagcggatt ccagccaata cctacaagtc ccaaggccgt ggcggcaaag gcattatggg
      961 catgaccact aaggaagagg atgtagtgga gcacttggtc ttaactcata atcatgactt
     1021 tatcctcttc tttaccaata agggccgggt tttccgccag aaggtatatg aaatcccagc
     1081 ggcctctcgt accgctaaag gccaggcaat cattaatcta ttacagcttg gaccggatga
     1141 ggttataacc tcgctcaaca cctttaatac cgccagtgcc ggcaaatacc tatttatggc
     1201 taccaagaac gggaccgtta agaagaccgc cttagccgat tatgccaatg tgcgcgccaa
     1261 cggcttaatt gcgattaagc tggatgaggg cgatgaactg cgctgggtca agcaaacctc
     1321 tggtacagat gaaatcatta tgtctacagc tctggcccag gctattcgct ttaaggaatc
     1381 cgatgcccgc ccaatgggca gggcaacccg gggagttcgc gggattcgtt tgcgtcccgg
     1441 cgatcaggtt gtgggtgttg atttggtgca gcccggaagt aaactcctgg ttattatgga
     1501 aaatggctac ggcaagcgga catcggtgga tcagttttca acgcatgctc gcggcggtgt
     1561 gggtataaag gccggcgtgg ttaccgctaa aaccggcaaa gcgctggatg tccgggcact
     1621 tgaaaatgat gatgaagacc tggtcattat ttcctcgagc ggacaggtta tccgactgcc
     1681 tattaaaacc gtatccttga ttggtcgggc aacacagggt gtgcgcataa tgcgcttaaa
     1741 ggatagcgat aaagttgcat caattgcgct tattggcgaa tcaaagcttg aactggaaat
     1801 tggcgttgat ggcgaatttg aatcaagcga tgaagccgaa gccgaaactg atgctcggcc
     1861 agcctccccc ccagcataag ctttttccat ttttttaagt aatcatcaaa aaccctcgta
     1921 tgaaacacag aagagggttt ttggctatac gaagcctaat atgctaatgc ttacaccgct
     1981 taaataattg gtcataatta caaaaatatt aaatttttat aaataccttt tgttatttta
     2041 acggtattgg ttcctgatga taaggcatat gctgtttaca tagattaagt aagtgatgtt
     2101 aaggaggccc ttgtagctaa gggactttgg tggagccaga acgtatgccg caacgcttgc
     2161 tgctaaaact cggggttatt ttcctggctg gtgccatggg gagtggtgcc gccttggcga
     2221 gcgaagaggg gagtcccgcc gccacaacca gtccgggcgt gagcgctgag cagcaaagcg
     2281 cgcaggatgt tgtaatcact acatcggatg atgccgccat tacggtttca tcgactacct
     2341 ctcagagcca aagtgtggtt ggctctagcc agtcaagcac cgatgcgggt tcggcagccg
     2401 cggtttcagc aggagcaggg cttgaagcaa ccgctagtga tctcgaagca aaatctgctg
     2461 tgcttaatga tgacacatta tcaggaacac ccgtacatgg cgcctcaagc ctaagcgggg
     2521 gattcacgtc tgcgggtgcc gaaaagcctt ccgttcaagc agcggcagga gcaaaggccg
     2581 agaaccaggt tcgtgttgct cttgcttggg cagcctatca aatggtgacg cggaatgttg
     2641 cggctttaca gcccattgca cctattgagt tgactccact atctaccggt agtaatcttc
     2701 ccgctcttcc tcaaaatcct actggctggc tcggcgatct gagagtactc ttaacctcaa
     2761 gcctgctccc gctactcggt gaattggtta gtcagccggg aagcatggca tcagccgtaa
     2821 cgctgttgtc attgaagtta atcttattga tacttttggt gattgcagct tctctgttac
     2881 ttcttgattt ttacacggca cgtttgcgcc gctcgggata taccgatgca gcgcgtagcg
     2941 acgc
//
LOCUS       DASVGB010000056         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_173346, whole genome shotgun sequence.
ACCESSION   DASVGB010000056 DASVGB010000000
VERSION     DASVGB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173346"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>2362
                     /gene="ileS"
                     /locus_tag="VF272_01665"
     CDS             12..>2362
                     /gene="ileS"
                     /locus_tag="VF272_01665"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7259619.1"
                     /translation="MGVDTFNAAARASVFKYKSEWEDFMRRLGRWADIENSYATMDDD
                     YIESVWWVMSRLHQKDLVYRGFRSSPYCPRCATPLSNFEVNQNYKDNVSDPSVYVKFQ
                     VEGDEKTSLLAWTTTPWTLPANAALAVKADADYVTVAMTHDGDGWVKGERLILAKGRI
                     GELDLRKAEYRIVETRQGSELVGTKYRPLYPRLVEQRLLSDIEREKVHKVYRDDSVSL
                     EDGTGILHVAPRYGETDLELGLREDLPLIESVDGNGLMSAYLKDIEDPFLATIAGMFF
                     KKADNHIIEDLAKQHRIFAAETFEHTYPFCWRCETPLMYFALPSWYIKVSELRGQLVG
                     NNDQINWIPEHIKVGRFGKWLADARDWNFSRNRFWGAPLPIWVNDHNSEDIIVVGSLD
                     ELRKLSGHTGEFDLHRPGIDEVVIKRDAKTYRRTEEVFDCWFESGSMPYAQDHWMGGQ
                     SGEELAPHFPAQFIAEGVDQTRGWFYTLHVLATALVDKPAFENVVVNGLVLAADGKKL
                     SKRLRNYPDPSEIFATSGADSLRFFLMSSPVVGGEDVRFSAQAVSEVNRNVFTRLWNV
                     HSFFSTYAEIDKWQPEKELREPKSDNVLDQWLLARLNQVIAEMTEQADNYRIASAVRP
                     LRDFIDDLSNWYVRRSRRRFWKSEDDGDKASAYATLHYAMVRTSQLLAPWSPFISDKL
                     WRELTVGMDVPASVHLSDWPEAGEVNSLVVSDMNNVRQIVNEGLAARAKAGIKVRQPL
                     NAITVTGNVTLSPESREIVADELNLKSVNMTVKDLEDGVKYGP"
BASE COUNT          562 a          558 c          685 g          557 t
ORIGIN      
        1 aaatcttaga gatgggtgtc gatacgttca acgctgccgc aagagccagc gtgttcaagt
       61 acaagtcaga gtgggaggat ttcatgcggc gcttaggacg ttgggctgac atcgaaaact
      121 cctatgcgac catggacgac gactacattg aaagcgtctg gtgggtaatg agtcggttgc
      181 accaaaaaga tctggtgtat cgcggttttc gttccagtcc gtactgtccg cgctgcgcca
      241 ccccgcttag taattttgag gtaaatcaaa actacaaaga taatgtgtca gacccgagtg
      301 tatatgtgaa gtttcaggtt gagggtgacg aaaagacctc gctactagcg tggaccacta
      361 cgccgtggac tctgccggca aacgctgcct tggcggttaa ggctgacgcc gactacgtga
      421 cggtggcgat gactcatgac ggtgacggct gggtgaaggg cgaacgactc atcctggcta
      481 aaggccgcat tggcgagctc gatttacgca aagccgagta taggatagtc gaaacgcggc
      541 aggggagcga gctggttggt acgaagtacc gcccgttgta tcccagacta gttgagcagc
      601 gtttgctttc tgacatcgaa cgagaaaagg tacacaaagt gtaccgggat gattcggtca
      661 gcttggaaga tggaaccggt attttacatg tggcgccgcg ctacggtgaa accgatcttg
      721 aacttggcct gcgcgaggac ctgcccttga tagagagcgt agacggtaat ggtttaatga
      781 gcgcctacct taaggatatc gaagatccgt tcctggctac tattgccggc atgtttttca
      841 agaaagccga caatcacatt attgaagacc tcgccaaaca acaccggatt tttgccgccg
      901 aaactttcga gcacacctat ccattttgct ggcgctgcga aacccctctg atgtatttcg
      961 ccctcccaag ctggtatatc aaggttagtg agctgcgcgg gcagctggta ggtaacaacg
     1021 atcagattaa ttggattccc gagcatatta aggtcggccg ttttggtaaa tggctggccg
     1081 acgcccgcga ttggaacttt tcccgaaatc gtttttgggg ggcgccgctg ccgatttggg
     1141 taaacgacca caatagtgaa gacattatcg tggtggggag tctggatgaa ctgcgtaagc
     1201 ttagcggcca caccggcgaa tttgacctgc atcgtcctgg cattgatgag gtagtgatta
     1261 aacgtgacgc caaaacctat cgccgcaccg aagaggtgtt tgattgctgg tttgagtccg
     1321 gttcgatgcc ctatgcccag gatcactgga tgggtggcca gagcggggag gagctggcgc
     1381 cgcattttcc ggctcagttt attgccgagg gagtcgatca gacccgcggc tggttttaca
     1441 ccttgcatgt acttgccacc gccttggtcg ataaaccggc atttgaaaat gtggtcgtaa
     1501 acgggctcgt gttagccgcc gacggcaaaa aactgagcaa gcgtttgcgc aactatcccg
     1561 atccatctga aatttttgca acctccggcg ccgacagcct gcgctttttt ctgatgagta
     1621 gtccggtggt agggggcgaa gatgtgcgct tttcggcgca agccgtcagc gaagtaaacc
     1681 gcaatgtctt tacccgtttg tggaatgtgc atagcttctt ttcaacgtat gccgagatcg
     1741 ataaatggca gccggagaaa gagttgaggg agccgaaatc ggataatgtg ctcgaccaat
     1801 ggctgctggc tcggctgaac caagttatag ccgagatgac agagcaggct gataattacc
     1861 ggattgcttc ggcggtccgg ccgttgcgcg actttattga tgacctatcc aactggtacg
     1921 ttcgtcgcag ccgccgccga ttctggaaga gcgaagatga tggcgacaag gcttccgcat
     1981 acgctacatt acattacgcg atggtccgca ctagccagct gctggccccg tggagcccgt
     2041 ttatctccga taagctctgg cgagagctga cggtaggcat ggatgtgccc gcttcggtac
     2101 atttgagtga ctggccagaa gcgggtgagg taaacagcct ggtcgtttcg gatatgaata
     2161 atgtcaggca gattgtaaat gaagggttag ctgcccgtgc gaaagcaggt attaaagtaa
     2221 gacagccctt gaacgctatc acggttaccg gcaatgtcac ccttagtccc gagtcacgcg
     2281 agattgtcgc tgatgagctt aaccttaaat cagttaatat gacggttaag gatctggaag
     2341 acggggtaaa atatgggcca at
//
LOCUS       DASVGB010000057         7670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1102175, whole genome shotgun sequence.
ACCESSION   DASVGB010000057 DASVGB010000000
VERSION     DASVGB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102175"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..347)
                     /locus_tag="VF272_01670"
     CDS             complement(12..347)
                     /locus_tag="VF272_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259620.1"
                     /translation="MSAAEPFQWQIAIPSDDGSLAQLEAAKDRLSQIAFSGGTSDTEG
                     GIALYEALLISIMLSSSEPIIDFKHYMELTCLPLRSEDHHDERVKAWNRVCRFNYQGA
                     SLEPWIRSA"
     gene            complement(432..914)
                     /locus_tag="VF272_01675"
     CDS             complement(432..914)
                     /locus_tag="VF272_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259621.1"
                     /translation="MENPKPRFEDKSVESLDSQLPVFEKEVAAPETVSSLVTAIKEKL
                     QDVSTVEGDGSNEKIAKFEVVQNPDSPGNVTLTLAMNSADDDSVEIYLEEPAFDTATG
                     RFTGDMVHATYGSEHTDTIEHPYRLVLDLDDGAADISKDDAEKLLAYIANAKRVESPL
                     "
     gene            complement(991..1099)
                     /gene="rrf"
                     /locus_tag="VF272_01680"
     rRNA            complement(991..1099)
                     /gene="rrf"
                     /locus_tag="VF272_01680"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1298..4789)
                     /locus_tag="VF272_01685"
     rRNA            complement(1298..4789)
                     /locus_tag="VF272_01685"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(5129..5419)
                     /locus_tag="VF272_01690"
     CDS             complement(5129..5419)
                     /locus_tag="VF272_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259622.1"
                     /translation="MSEQAGPGPEDNETGSFPQTPSELADKIRAAEATPATAANYADD
                     LATLRFQRNQDEAEAARVNADEKRAAAGLPPLNAESATEDMPSENQEHREAA"
     gene            5498..5755
                     /locus_tag="VF272_01695"
     CDS             5498..5755
                     /locus_tag="VF272_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259623.1"
                     /translation="MFESEEHHEENEAGPEDHSVGQETDVELTQEAADENVPVAEDEP
                     AEADPLPSPEALVESGDVPDPEEDNASPGYTSPESDQNKEA"
     gene            complement(5756..7267)
                     /gene="guaB"
                     /locus_tag="VF272_01700"
     CDS             complement(5756..7267)
                     /gene="guaB"
                     /locus_tag="VF272_01700"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7259624.1"
                     /translation="MAETNHSNSDLRHIPEALTFDDVLLVPAYSQILPSEADLRTHLT
                     KKITLTMPLLSAAMDTVTESRTAIAMARLGGLGVIHKNLSIDEQVAEVKKVKKSEAGL
                     IVHPIVVAPDATVAEIRATMAEAGISGAPVTENGDADGRLVGIITSRDLRFVDDNTLK
                     VADCMTQAPLVTAPTGTTLEEAHKIFQAHRIEKLPVVDSDGRLHGLMTIKDITKQQDF
                     PGAVKDELGRLLVGAAIGVGDAGMERAGALVEAGIDVLFVDSAHGHSQGVLNAVKALK
                     DRFGNKVEVVGGNVATAEGAEALMKAGADAVKVGIGPGSICTTRVVTGIGMPQLTAVQ
                     DAVHACKKHNVPVIADGGIKYSGDITKALAAGANVVMIGSLFAGTEETPGETILYQGR
                     SYKTYRGMGSMGAMGGGEGAKERYFQENVNDSDKLVPEGIEGQVPYRGPLRETVHQLM
                     GGLRSGMGYCGAETIPDLQKTAQFVRITNASLIEGHPHDVKITKEAPNYRLKG"
BASE COUNT         1721 a         2091 c         1806 g         2052 t
ORIGIN      
        1 tctcccttga gttaggcgct ccggatccag ggctcaagtg aagcaccctg atagttgaac
       61 cgacagaccc gattccaggc cttgacccgc tcatcgtgat ggtcctccga acgcaagggc
      121 aagcaggtca actccatgta atgcttgaag tcgatgatcg gctccgaaga actgagcatg
      181 atgctgatca ggagcgcctc gtagagagca atcccgcctt cggtatcgct cgtgccgcca
      241 gagaaggcga tttggctcaa ccgatccttc gcagcctcca gctgcgccaa cgagccatcg
      301 tccgagggta tggcgatctg ccactgaaac ggttccgctg ccgacatgtt cccttccctt
      361 ccacaagttg gacgaagtga cgtaaacttt agcgaatcga tacctgggtg tcaagcatac
      421 cgaagcatgc tttagagggg actttctacc cgctttgcat tggcaatata tgctaacagt
      481 ttttcggcat catccttgct gatatccgct gccccatcat ccaggtctag caccagccta
      541 tagggatgtt caatagtatc ggtatgctca gacccatacg tggcatgtac catatctccg
      601 gtgaatcttc cggttgcagt atcgaacgcc ggctcttcca ggtatatttc gactgaatcg
      661 tcatcggccg agttcattgc caatgtcaac gttacgttcc ccgggctgtc ggggttttgg
      721 acgacctcaa acttggcaat tttttcattc gacccatcgc cttcgaccgt tgagacatct
      781 tgcaattttt ctttaatagc ggtcacaaga ctgcttaccg tttccggcgc agccacctcc
      841 ttctcgaaaa ccggaagctg cgaatccaga gattcaaccg atttatcttc aaacctgggt
      901 ttcgggtttt ccataatagg cagtatatca gcttcactca taagggacta taacgcaaag
      961 agacgctctt ttcagggcgt ctctttgata atccggcact gtgctacttt cccgctaatg
     1021 cagtatcatc gccgcagatg agcttaactt cggtgttcgg gatgggaacc ggtgtgacct
     1081 caccgctaaa agcaccgggc accgacctga aagcccaaat acttcttctc gaatgcctac
     1141 tatgcattct gatctatgat cagacgatat gtggaggcag ggaagaacgt atttgtacgc
     1201 tttcagaaca ctacccgaaa ctcttagaga gttcggatgt aacttttttt ggtacatggc
     1261 tattttataa gccacaaatc gctagtcaaa tcacgtagta aaaaagtcaa tggaccgatt
     1321 agtacacctc ggctgaacgt attactacgc ttacacctag tgcctatcaa ccagatagtc
     1381 tttctggggt ctcatgggga aatcttatct tggagatggc ttcgtgctta tatgctttca
     1441 gcacttatcg caaccgaact tagctacccg gcaatgccgt tggtacgaca accggtacac
     1501 cagaggttcg ttcatcccgg tcctctcgta ctagggacaa acctccgcaa atttccttac
     1561 gcccacacca gatagggacc gaactgtctc acgacgttct gaacccagct cgcgtacccc
     1621 tttaattggc gaacagccaa acccttggga gctgcttcac ccccaggatg ggacgagccg
     1681 acatcgaggt gccaaacagc gccgtcgctg tggacgcttg ggcgctataa gcctgttatc
     1741 cccggggtag cttttgtccg ttgagcgctg ccgattccac tttcttgcag aataaattct
     1801 acagtgagac agcggatcac tagctcctgc tttcgcacct gctcggcttg taggcctcgc
     1861 agtcaagctg acttatgcgc ttgcactatc gatacgattt ccatccgtac ctagtcaacc
     1921 tttgaacgcc tccgttactc tttaggaggc aaccgcccca gttaaactac ccaccagaca
     1981 ctgttcctga accggaacaa acgggcaaac aatttaacat aagcattgtg cgaaagactt
     2041 taaaactact aacaagggtt agttttagta attgggtgtt ggtgcgctgg cgcgtcgctt
     2101 caggctgaaa cagcttgaag catacgcttt cgcaaccaaa ccaatcacta actaactctt
     2161 acgtgttgct ttttttacaa tgcttatgct tgctgaagaa tgcaatcttt ttctattcga
     2221 tgtctacgca atctgttcta tgaacagacg ttatgtgaca gcaaagaaaa atgattgatc
     2281 tcacagcttt gtccgcttat tgcggtccag gttaggacaa ggttgcagta agggtggtat
     2341 ttcactggcg actccaccga agctagcgcc tcggcttcaa agtctcccac ctatgctaca
     2401 catacaacaa cccagcccaa tgccaagctg tagtaaagct ccacggggtc tttccgtctt
     2461 ggtgcgggta aacggcatct ttaccgctat tgcaatttca ccgagtccct tgctgagaca
     2521 gtgcccagat cgttacgcca ttcgtgcggg tcggaactta cccgacaagg aatttcgcta
     2581 ccttaggaca gttatagtta ctgccgccgt ttaccggggc ttcatgtcgc agctctcacc
     2641 gctctactta accttccggc accgggcagg cgtcagtccg tatacatcca ctttcgtgtt
     2701 ggcacagacc tgtgtttttg ctaaacagtc gcctgggctc tttagctgcg gctgacattg
     2761 ctgccagcag accttatccc gaagttacgg tcgctatatt gccgagttcc ttagcaaggg
     2821 ttctctcgtt catcttagtc tactcgactc gagcacctga gtcggtttgc ggtacgggcg
     2881 gccacggtta ttacatatgt aagcttttct ggtcggcttg ggtggcccga attcaccttt
     2941 ttaggggcgt tttattcacg cttcggtctc agcaccccgc attcgagata tgtcgcttcg
     3001 cgacaattac tccaatgcaa agtgctgagc cctagacgct taaacggata taaccaaaaa
     3061 tccgctcgga ttaccatgcc gcgcactaca tacaacaccg ttgccggtac gggaatatta
     3121 acccgttgtc catcgcctac gctactcgcc tcggcttagg cccgactaac cctgggacga
     3181 taatcgttgc ccaggaaacc ttgctcttac gatgtgcagg attctcacct gcattatggt
     3241 tactcattcc agcattctca cttccctgcg ctccaccagt ccttccggac tggcttcaat
     3301 gcccaaggaa cgctccgcta ccacgcttgc attgctgcag catccgtgtc ttcggtagta
     3361 tacttagccc cgttatattt tccgcgcaga gtcaccattg accagtgagc tgttacgcac
     3421 tctttaaatg catggctgct tctaagccca catcctggtt gtttatgcgt ctccacatcg
     3481 tttcccactg agtatacatt tagggacctt agacgacggt ctgggctgtt tccctcttga
     3541 gcgacgagct tagccccgcc gttctgactg ccacggtagt tcacttcggt attcgcagtt
     3601 tgacaggggt gggtatcctt tcggacccca gacccaatca gagctctacc cccgaaggag
     3661 agttcgtgac gctagcccta aagctatttc gcggagaacc agctatctcc gagttcgatt
     3721 ggaatttctc cgctaaccac aggtcatcca aaagatttgt acctcttcct ggttcggccc
     3781 tccacgtagt tttacctacg cttcagcctg cccatggtta gatcacccgg tttcgggtct
     3841 aatcccgtgt actaaatcgg cctattaagc ctcgctttca ctacggctcc gtcatatgac
     3901 ttaaccttgc acacgaaatt aactcgctgg atcgttctac aaaaagcacg ccatcacaga
     3961 ataaatctgc tctgactcat tgtaagcaca tgatttcagg ttctatttca ctccccttcc
     4021 ggggttcttt tcgcctttcc ctcacggtac tagttcgcta tcgatcgtaa agagtattta
     4081 gccttagcgg gtggtcccgc cagtttcaac cagggtttca cgtgtcccgg cctactcgag
     4141 aatacaaaca taaggacaaa gaatttttag ctacgggact gtcaccctct atggtgtggc
     4201 tttccatcca cttctcctaa tcctttattt tcttacctta cccggacttt ggcagttgcc
     4261 ggtcgcaaag agaagggctc ataaaagcac ttctctttgg tcgtttgtat ctcacaaccc
     4321 cttcctagca ttcgcctgcc agcttattta ctcctcattc agtcacgata ttgacaccct
     4381 cagataacca attttccagt gaggactgtt tgagcaccac taggtgcgag ttccgcagcg
     4441 gaaaattggt tgctgagtag gcaatattag atcttatata tcgtaactaa atgagaagca
     4501 aaagttaaaa aggttttggc tcttcccgtt tcgctcgccg ctactcgggg aattgaggtt
     4561 tctctctttt cctgtgggta cttagatgtt tcagttcccc acgtgccctc ctactatcct
     4621 atatattcag atagtggtaa ccgcgtataa ccacggctgg gttgccccat tcggaaatcc
     4681 ccgggtcaaa gcttgattgg cagcttaccg aggcttatcg cagccttcta cgtccttctt
     4741 cggctcttta cgtcaaggca tccgtcatgt gctcttgagt aactttttta cgttgattga
     4801 ctccgcgcaa acccaaatgt tcgcgcttcg tcggtttgac tagcgatttg tggttacaaa
     4861 agtaacttca aaatcgcttg gtttatttgc attaccctcg atgatatctt gaagcagata
     4921 ccatcgatat tcaattttca aaaaacaact ccgccttttc ttacttggcg gagcccacat
     4981 tcacttaaac aatcaagtaa ctaggttcta gaacgagcct ccgttattac tggctgattg
     5041 tttacgcata tgtgagctct cacttaaagt ctgtagccac tttagcaggt ttggaccgct
     5101 tgtgtaaagg ggttttttga cgtgtgaatt atgccgcttc gcggtgttct tggttttcgg
     5161 atggcatgtc ttcggtggcc gattcggcat ttaggggagg caatccggct gctgcacgct
     5221 tttcatccgc atttacccga gccgcttcag cctcatcttg gttgcgttga aatcgtaagg
     5281 ttgccaaatc atcggcatag ttagccgctg ttgcaggtgt tgcttcagct gctcgaatct
     5341 tgtcggcaag ttcgctgggt gtctgtggaa atgatcccgt ctcgttatcc tctggtcctg
     5401 gtccggcttg ttctgacatg gttgttcctt ttgttcttga atttgattat agcatcttgg
     5461 cggagtcatc tataatgagg aaaacggagg aattaggatg tttgaatcag aagagcacca
     5521 tgaagaaaat gaggctggcc cggaagatca cagcgtcggc caagaaactg atgttgagtt
     5581 aactcaagaa gcagcagatg aaaatgttcc cgtggcagaa gatgagccgg ctgaggcgga
     5641 ccccctccct tcacccgaag cactggttga atcgggtgat gtccccgatc ctgaggaaga
     5701 taatgccagc cccggatata cgtcgcctga aagcgatcag aacaaggaag cctaattacc
     5761 cttttagacg gtagttcgga gcttctttgg taatttttac gtcgtgcggg tgtccttcaa
     5821 tcaggctagc gttggttatg cggacaaatt gagcggtttt ttgaagatcg ggaatggttt
     5881 ctgcgccgca gtaacccata ccgctacgta aaccgcccat aagttggtga acggtttcgc
     5941 gcaagggccc gcggtaaggc acttggcctt caatcccttc cgggaccagc ttatcgctat
     6001 catttacatt ctcctggaag tagcgttctt tggctccctc accgccaccc atggccccca
     6061 tgctgcccat gccccggtag gttttatagg aacggccttg gtacagtatg gtttctccgg
     6121 gcgtttcttc ggtgccggca aataacgacc cgatcatgac aacgttggcg ccggcggcta
     6181 aggccttggt gatgtcgccg ctatacttaa tgccgccgtc ggcaatcacc ggaacgttgt
     6241 gttttttaca cgcatggaca gcatcttgca cggcggtgag ctgcggcatt ccgataccgg
     6301 ttaccaccct ggtggtgcaa attgatcccg gaccaatccc aaccttcaca gcatcggcac
     6361 cggccttcat taaggcttcg gccccttcgg cggtagccac gttgccgccc accacttcga
     6421 ccttgttgcc aaaccggtct ttgagggcct tcacggcgtt aagcactccc tgtgaatgtc
     6481 cgtgagcgct atcaacaaac agcacatcga taccggcttc aaccaacgcc ccggctcgct
     6541 ccatgccggc gtccccaaca ccaatggctg ctccaacgag caagcgtcca agttcatcct
     6601 taaccgctcc gggaaagtcc tgctgcttgg taatgtcttt aattgtcata agcccgtgca
     6661 ggcgaccgtc actatcaacc accggtagtt tctcgatgcg atgggcttga aagatcttgt
     6721 gcgcctcttc gagagtagtg ccagtcgggg cggtcaccag tggagcctgc gtcatgcaat
     6781 cggccacttt tagcgtatta tcatctacaa aacgtaagtc gcgactggta ataataccaa
     6841 ctaagcggcc gtcggcatcg ccattttcag taaccggagc accggaaata cctgcctctg
     6901 ccatggttgc acgaatttcc gcaacggttg catccggcgc tactacaatc ggatgaacga
     6961 tcaatccggc ttcgctcttt tttactttct taacttcggc aacttgttcg tctattgata
     7021 gattcttatg aatcacaccc aagccgccta aacgggccat ggcaatcgcg gtccggcttt
     7081 cggtaacggt atccatggct gccgatagca gtggcatagt cagcgtgatc ttttttgtga
     7141 gatgagtccg aaggtcagct tcagatggga gaatctgcga ataggctggc acaagcagaa
     7201 cgtcgtcgaa ggttagggct tctggtatgt gacgtaaatc gctgttgcta tggttagttt
     7261 cagccattgg ctccctcatt tgtattaaaa aactataaca caaaccacaa gaataataaa
     7321 gcctattatt actggagtta tacttcccgg gacgcatggt gcgccccggg aagcggtccc
     7381 cttcgatccg agatttgcat gcaaacgaca tccgagatct cgctgaggac gagaaggaag
     7441 ttggctctag gcctggtttg cacgactcgg gaggaggcgg caccgggata tggggtgcct
     7501 cagacacgaa cggtgaactg ctgattcggg gctcgtgtct ggcgcaggag gcatgctagg
     7561 gacatgccgc aagctgcgac tttcgtccca ccatgtgggc tggaacgaat ctgtaaaaac
     7621 tggtagcccg gcggggttcg ctgagcaagc gatcccctcc cagccgggct
//
LOCUS       DASVGB010000058         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_505893, whole genome shotgun sequence.
ACCESSION   DASVGB010000058 DASVGB010000000
VERSION     DASVGB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505893"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1109
                     /gene="ftsZ"
                     /locus_tag="VF272_01705"
                     /pseudo
     CDS             162..1109
                     /gene="ftsZ"
                     /locus_tag="VF272_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            1554..2027
                     /gene="nrdR"
                     /locus_tag="VF272_01710"
     CDS             1554..2027
                     /gene="nrdR"
                     /locus_tag="VF272_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX7259625.1"
                     /translation="MKCPKCEHEASKVMESRDLEDGTSIRRRRQCLNCSHRFTTYERL
                     ERPPLAVIKKDGRRELYRREKVTGGLYRACEKRPIPAEVIEAIANQIEQEVRSLGEPE
                     VTVGQVGEIVMNKLLELDDVAYVRFASVYRAFTDVASFEAELARLKRRIDSPANP"
     gene            2058..2318
                     /locus_tag="VF272_01715"
     CDS             2058..2318
                     /locus_tag="VF272_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259626.1"
                     /translation="MNLYILYTKDTPADRDISYLQRRLDERRISWKAIDADSRDGAAK
                     TELYDIMRRPAVLVTDSSGGVMQKWEGELPRLEDIITYYPAG"
     gene            2318..2578
                     /locus_tag="VF272_01720"
     CDS             2318..2578
                     /locus_tag="VF272_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX7259627.1"
                     /translation="MVAVLVNFINLFVTVFQVLILIRVIMSWVSPHPSSRIGLLIMEL
                     TDPLLLPIQKVLPKTQMFDFSPLVAFLLLQLLSVFANQLGTT"
BASE COUNT          693 a          611 c          725 g          670 t
ORIGIN      
        1 ccaatggtga aagtgcgtgt ataacagctt ttcaacggat ggcaaataat gattgtatac
       61 aaaaattatt cagttattta tccccaaaaa ttgtgaataa acaatttgca tttttaaacc
      121 gctatcgcta gactgattct cagtggtaag gagcattata aatggctgag gttcttccgg
      181 agattcaaac cttcgcccgc atcaaagtgg ttggtgttgg agggggtggc acaacagcag
      241 ttgagcgaat gattagctcg aggttgcagg gaattgagtt catagcaatt aataccgatg
      301 cacaatcatt gcatcactcg catgcacagc aaaaggtgca tattgggcag agtttaaccc
      361 ggggtctcgg tgccggcgcc aaccctgatg tcggccgtaa ggctgctgaa gagaatcacg
      421 cagacataag tgatgcgcta aaaggcgtcg atatggtttt tataaccgct ggcatgggcg
      481 ggggtaccgg taccggtgca gcacctataa ttgctgcggc tgcgcgagag tcgggtgcat
      541 tagtggtagg tattgtcacc aagccattta cattcgaagg cgagcgtcgc atggatcaag
      601 ctgagcatgg cattgaagaa atgcgcgaag cggttgatac gctgattacg ataccgaatg
      661 atcgcttgct cgatatggtt gatcggaaaa catcgctgct tgatgctttt aaggtggttg
      721 acgatgtttt gcagcaaggt gttcagggta tttccgattt aataaccgtc cacggattga
      781 tcaatctcga cttcgccgat attaagtcga ttatggagaa cgccgggtcg gcgcttatgg
      841 gaattggccg ggctaaaggt gagaaccgtg cggttgaagc cgcccgtcag gctatcgatt
      901 ccccgctcct ggaagtaaat gttgatggcg ccaagggtgt cctatttaat atcaccggcg
      961 gccggaacat gagcatgtat gagattgacg aagcggctaa gacaattacg gaagctgttg
     1021 atagcaacgc caatattatt tttggtgcgg tgctcgatga aaccatggat gacgacatta
     1081 aaattacggt tgtagcaacc ggatttgaaa caacgccgcg gcctccgcgt ccagcgttca
     1141 atccatcggt ggcaactccg agtcgagaat catataccga tgtgccaatg ccgtcattta
     1201 tggatgccga cccgttgccg gctccttcga gcgaaccggt agcaaatcgt tttaaggccg
     1261 aacctttcat taatgagcgt cctactttcg aggattcggg aactctagac gatgaaaact
     1321 ccctgtttaa tcaaccggtt cgcccggaag taaattccat agagccggaa gtcgatagga
     1381 gcgcagaaaa gccgggtcac cccaaagaga cttcggtcgc taaaagccgc actgccaaat
     1441 cattgattga acgcgccagc cgtgcagtca gcggtcatgg ccacgaatat aaagaagaag
     1501 atctagacgt tccggctttt ttgcggcgta aaaaataggg agataaacgc tacgtgaagt
     1561 gccctaaatg cgaacatgag gcctctaagg tcatggaatc acgcgatctt gaagacggta
     1621 catccatccg ccgacgtcgc caatgcctga attgcagtca tcgtttcacc acgtatgagc
     1681 ggctggagcg gccaccgctt gcagtcatta agaaagatgg tcggcgcgag ctgtatcggc
     1741 gggaaaaagt taccggcggt ttgtaccgtg cctgcgaaaa acggccaatt ccggccgagg
     1801 ttattgaagc catcgcaaat cagattgagc aggaggtccg cagcttgggc gagcctgagg
     1861 taacggttgg gcaggtgggg gaaatcgtca tgaataagtt gctggagctg gatgatgtag
     1921 cctacgtccg tttcgccagt gtttaccggg ctttcaccga cgtagcttct tttgaagctg
     1981 agttagcccg cctgaaacgc cgtattgatt ctccggctaa cccttaggaa gcctcagctt
     2041 tttgctatac taaagccatg aatctttata tcttgtacac caaagacacc ccggccgacc
     2101 gtgatatcag ctatttgcag cggcgtttag acgaacggcg tatctcttgg aaggcaattg
     2161 atgctgatag ccgtgatggc gcagctaaga cagagctgta cgacatcatg aggcgtccgg
     2221 cggttttagt aaccgattcg agcggagggg tgatgcagaa atgggaaggg gagcttcctc
     2281 gcctcgaaga tattatcacc tattatccgg ctggctgatg gttgcagttc tcgttaactt
     2341 tattaatttg tttgttacgg ttttccaggt tcttatactg attcgggtga ttatgagctg
     2401 ggtgtcgccg catccctcaa gccgaatcgg gctgcttatt atggagctga ccgacccgct
     2461 gttgctgccc atccagaagg ttttgccaaa aacccaaatg tttgattttt caccactggt
     2521 agcattccta ttactgcagc ttttaagtgt attcgccaac caactgggaa ctacgtaagg
     2581 tatgacccag catttcatag caaaggccga aggtgccaac tttcctgaat tcgaagctga
     2641 gatgcttaac gtctgggatg atgaaaaaac cttcgagcag tcacttaaaa accgtgaaa
//
LOCUS       DASVGB010000059         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_5660, whole genome shotgun sequence.
ACCESSION   DASVGB010000059 DASVGB010000000
VERSION     DASVGB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5660"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VF272_01725"
     CDS             <1..277
                     /locus_tag="VF272_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7259628.1"
                     /translation="DTGRGIPKDMQSLLFRKFQQAGSSLLTRDTTRGTGLGLYISKLL
                     GEKMGGKISLENSEPGKGTTFSFSLPLATSEQIKASREHEAQPKSSA"
     gene            322..1365
                     /gene="mnmA"
                     /locus_tag="VF272_01730"
     CDS             322..1365
                     /gene="mnmA"
                     /locus_tag="VF272_01730"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7259629.1"
                     /translation="MNIYVGLSGGVDSSVAAALLQHQGHDVTGVYMKNWTEDLPGMKC
                     PWQDDLRDARAVAATLNIPFKVFDFEAEYRSQVVDYMIAEYQAGRTPNPDVMCNQEIK
                     FKLFLDAALADGAEAIATGHYAHIDAGQLLRAEDDNKDQTYFLYRITSEALQKTVMPI
                     GDYTKPEVRKLAADFGLPTATKKDSQGICFVGPVGMKAFLKQYVAADPGPIMLHGQRI
                     GTHEGTVFYTIGQRQGLGVGGGKPYYVIGKDMAANTVFVTDDSADLDLQTDQIQLRDM
                     HWIGDAPDSSITLKARFRHRGELVECQLKEDLLVLAHTVSAIAAGQSAVIYKDAVCLG
                     GGIISASVAHAVR"
     gene            complement(1486..2064)
                     /locus_tag="VF272_01735"
     CDS             complement(1486..2064)
                     /locus_tag="VF272_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259630.1"
                     /translation="MAPPATHDAMQAALRSKLHDAVTAWNRDATFVETQALHEVIKNG
                     FSIKDVAKALGVPEDYVSFWLECDDEAIEFATPETAARLRWLRLIARYRDELQYKPYE
                     TRNWWRTVTTELRNRRPYEVFKTSPGDIVITMRSKYRIPPVRGKSNNDLLARLNLVPR
                     LAYVGASPAVRALAQQPYGQERKDIRNRRWHR"
     gene            2175..>2412
                     /locus_tag="VF272_01740"
     CDS             2175..>2412
                     /locus_tag="VF272_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX7259631.1"
                     /translation="MTTEQIRRKYLDFMKSKGHTVIPRANLVPQDDPTTLFTGSGMQP
                     LIPYLLGAEHPAGKCLVDSQPSLRAQDIEEVGDNR"
BASE COUNT          556 a          599 c          685 g          572 t
ORIGIN      
        1 agatactggc aggggcattc ctaaagatat gcaatccctt ctattccgca agtttcaaca
       61 agctggctca agtttgctga cacgcgatac taccagagga accgggctcg gtctctatat
      121 ctctaaactg ctaggtgaaa agatgggcgg aaagataagc ctggaaaatt ccgagccggg
      181 caaaggcacc acctttagct tttcgctgcc gctagccacc tcggagcaaa taaaagcaag
      241 ccgcgagcat gaggcccagc cgaaaagtag tgcctaacgt tactactagc ttgttttgcc
      301 taaaccgatt acgatgcaac tatgaacata tacgttggtt tgagcggtgg ggtcgatagc
      361 tccgtagcgg cggctctctt gcagcatcag ggtcatgatg tgaccggggt ctacatgaaa
      421 aactggacag aggatttgcc cggcatgaag tgtccctggc aggatgattt gcgcgacgct
      481 cgggcggtgg cagccacgct taatatccca tttaaggttt ttgattttga ggctgaatat
      541 cgaagccaag tggtggatta tatgattgcg gagtatcaag ctgggcgcac cccgaatccg
      601 gatgtgatgt gcaatcagga aattaaattc aagttgtttc ttgatgcagc tttggccgat
      661 ggcgcggagg caattgccac cggacattat gcccacatag acgctgggca gctgcttcgt
      721 gccgaagacg acaataaaga tcaaacctat ttcctgtacc gtataactag tgaggcgttg
      781 caaaagactg taatgccgat tggcgactac accaagccgg aagttaggaa gttagctgcg
      841 gatttcggtt taccaacggc caccaagaag gattcgcaag gaatttgttt tgttggcccg
      901 gtaggaatga aggctttttt gaagcagtat gtggcggccg atcccggtcc gattatgctt
      961 catgggcaga ggatcggtac gcatgaggga acggtatttt atactatcgg ccaacgccag
     1021 gggctgggtg ttggcggcgg caaaccttat tatgtgatcg gcaaagacat ggcggccaat
     1081 acagtattcg tgaccgatga ctcggcagac ttagatctcc agaccgacca gattcaattg
     1141 cgggacatgc attggatagg tgacgcacct gattccagta tcacactcaa ggcacgattt
     1201 cgtcatcgcg gcgaattggt tgagtgccag ctaaaagaag atctgctggt gctggctcat
     1261 actgtgtcag ccatcgcggc tggtcagtca gccgtcatat acaaggatgc tgtttgcttg
     1321 ggtgggggaa ttatctccgc ttctgtcgcc catgcggtta ggtagaaaaa agccccactg
     1381 ggcttgcttt cgcaatccta atggggcgga gaataccgtc cacccgccgt atcccggctt
     1441 tgcctttaag gggctcgaac cgaccatacg gcgggtgatt gacgtctagc gatgccaccg
     1501 ccgattccgg atgtcctttc gttcttggcc atagggctgc tgagctagtg ctcgaacagc
     1561 cggagatgcg ccgacatagg cgagccgcgg caccaggttg agtcgagcga gcagatcatt
     1621 gttcgacttt ccgcggacgg gagggatccg gtacttcgac ctcatggtga tgacgatgtc
     1681 gccgggactg gtcttgaaga cctcgtatgg gcgccggttt cttagttctg tcgtaacggt
     1741 tcgccaccag ttgcgagtct cgtagggctt gtactgcagt tcatctcgat accgggcgat
     1801 cagccgcagc catctgaggc gggcggcggt ttccggcgtt gcgaactcga tcgcttcatc
     1861 gtcacattcg agccagaagc tgacatagtc ctccggcacg ccgagagcct tggcaacatc
     1921 cttaatagag aaaccattct tgatgacctc gtgaagggcc tgggtttcga cgaaggtggc
     1981 gtctcgattc catgcggtta cggcatcgtg cagcttgctg cgtaaggcgg cctgcattgc
     2041 gtcatgagtg gcgggtggtg ccattctgac ctccgatcgc gtattgcgtg gtcatgcaaa
     2101 ggtgcactca atcatactgg gttttgctat ccggtgctac agcatctgga acaaaatacg
     2161 ctacaataat cgttatgact accgaacaga ttagacgaaa atacctcgat ttcatgaagt
     2221 ccaagggcca cactgtaata cctcgggcta atttagtgcc gcaggatgac cctacaacgc
     2281 tctttaccgg ctccggcatg cagcccttga ttccctattt acttggtgct gaacatccgg
     2341 cgggtaagtg tttggtggac agccagccaa gcctgcgggc ccaggacatt gaagaagtcg
     2401 gcgacaaccg gc
//
LOCUS       DASVGB010000060         7020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_445036, whole genome shotgun sequence.
ACCESSION   DASVGB010000060 DASVGB010000000
VERSION     DASVGB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445036"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..2456
                     /locus_tag="VF272_01745"
     CDS             63..2456
                     /locus_tag="VF272_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7259632.1"
                     /translation="MAKKRRIKPALFFEALPAAPQLPHAVVFDTERTKHLKRLGLLAS
                     RQLYNDTIALAHDIGLFVAIILRMLGGQTMRTIALFALSGMAIAGTIVAVRIGTAALS
                     EHGPSLASPAVIMGMKNTGTTILDKNGVVLYKGYGAVDRRSIPLFEMPPSVVQATLAA
                     EDPDFYKHPGVSWRSVARAAYRNASENGKIQGGSTITQQLVKNTLLTSERSFTRKYKE
                     MVLSMAMERRYTKDQILEMYLNTIYYGQGAYGIQTAAQTYFHKPARELTLGESALLAG
                     LPQSPSRFDPNINEEGAKKRRDYVLSRMQELGYAENVAVSAAKLQPIDAGSHQVVLKA
                     PHFVFYVLQQLRREYGEKTVKEGGITVTTTLDYIKQQEAELIVKKQITKLVAHNSTNG
                     ALVSLNPKTGDIISMVGSVDYNAPGFGNVNVTLSELQPGSSFKPIAYAVAFSKGWNGA
                     TVVDDKPVRFPQPSGEVFAPQNYDLKFRGQVLLRRALANSFNIPAIEVLQHGGLEATL
                     AMAHNLGIRPPSLTETNRYGLSLVLGGGEVRPIDMAAVYATFANGGKNVAPQAILEVK
                     DRAGNSITKPRTVPTRQAIDPRIAFMITNILSDTEARKEEFGARNPLQLSRPAAAKTG
                     TTNDFRDNWTIGYTPQLATAVWVGNNDHSPMLNVDGITGAAPIWHDYMEMALADSPVE
                     QFTPPSGLAFTKVCPIDGGLAAPNDPSAIEEVFLVENQPTKRCGQIRPPAVLVQAVTP
                     QNPTPPTPGSPSEAPPLLPPEPGRGGGGDDGDRGTPTPDPNQQPVSPPPSTFPFKKQ"
     gene            2556..3584
                     /locus_tag="VF272_01750"
     CDS             2556..3584
                     /locus_tag="VF272_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX7259633.1"
                     /translation="MSDTIVPQSAFNYVQTAEAILGALRNERDRQIVAQRYGLGLPKR
                     HTLEKIGSQFGITRERVRQIEKAATVKLQQTASTELTRIDNALKQHLARNGQVDLLQD
                     VAAAVGAHTPAQQAYISFLAALAPSIEIIEDSDHYHSALSTLPEYHKQHIAEITSHLV
                     HHLKQKAKPTPIDALHKAIPYDISKQTTQQVARVSKRLAHHDGHWGLSHWPEVNPRSI
                     RDKTYLVLGKHGQPLHFTDIATHIAGLGNQKRSVTTQAVHNELIKDPRFVLVGRGIYA
                     LSEWGYTPGTVADIITEVLRDESPLHKDEIVKRVLQKRQVKTTTIVLNLQEKDQFIRV
                     SKATYALK"
     gene            3689..6475
                     /locus_tag="VF272_01755"
     CDS             3689..6475
                     /locus_tag="VF272_01755"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX7259634.1"
                     /translation="MDFLITILFAVMQIIGTILFTYYGWVIVVAIFLYLIWQNRRREQ
                     WVGQIEHILLQIEVPKENEKKELSAEQMFASLHGILRSKSDLRKEGSLQEHISFEIAV
                     INDQIRFYVWTPRHLKNFVEGQIYAQYPNVQITEGIADYAKTELGSRQAYTTVLGLTK
                     TELLPIKTFQSFEVDPLAGITATLANMEDPGEEMWIQILARPIDDDWHSKGLNYIEDI
                     KEGKTHEPFEQMIFKALLAIPGYILTNFLGALVTPPGEAKEVKKELKATTGDQALAKA
                     AEEKISKLGYQVYIRIVYLGNDPIVARQRNQAIIGGFKQFNTTNLNGFYAESSKEGTG
                     GVDAYRARLFTSSGYILNIEELASVYHLPHKSVETPNMVWTTTKTSEPPPNLPTELTA
                     EPTGISLFAATNFRGKQLKFGMKRIDRGRHLYIIGQTGTGKSKLLNLLSLSDIYHGEG
                     MAIIDPHGDFATDMLQYVPEHRLQDVIYFNPTDRDFPIAFNPLEVYDQAFKNDISSEM
                     IGVLKRMFESWGPRLEYILRFTLLALLDYPDATMLGITRMLTDKEFRKKVIKEIKDPV
                     VKAFWVTEFASWNDKFANEAVAPVLNKVGAFTANPLIRNIIGQPRSRLDLRKIMDEGK
                     ILVVNLSRGQIGEDNASILGALMVTKIQLAAMSRANIPLDQRRPFYLYVDEFQNFATD
                     SFATILSEARKYALNLTVANQYVSQMPEEVRSAVFGNVGSIVSFRVGVDDAPIIAKNF
                     APLFEPDDLGKLHNQHILIDMSIDGEKSMPFSAKTLRMPDPEYDFTPQLIELSRQHYA
                     SDRTQVEQEISEWTLGQAATEKPSAEQEATPAPTPHEQKPNSFLGALKNPEPPPASQP
                     VHAAAHNPGSTGGNRNKRRGRSGQRNQNEQMGNRSGQQASVGAGTPVRMETRAHERAV
                     RPEESVVMRGPAGE"
BASE COUNT         1887 a         1816 c         1711 g         1606 t
ORIGIN      
        1 tgctttttcg ttgtggagca gaacaaaaaa gctgactaat gcggaggtgc tgacaaccca
       61 atatggcaaa aaaacggcgc ataaaaccgg cattattttt tgaagcatta ccagccgctc
      121 cgcagttgcc gcatgccgtc gtttttgaca cagagcgcac aaagcacctg aagcgccttg
      181 gtttattggc aagccgtcaa ctttataatg acaccatagc cttggcccat gatatcggcc
      241 tcttcgtggc cataatcctg cgtatgctgg gaggccaaac aatgcgtacg atcgcgcttt
      301 tcgcgcttag cggcatggca atagccggta cgattgtggc agtgcgtatt ggcacggcag
      361 ccctttcgga gcatggacca agtcttgcaa gtcctgccgt tattatgggg atgaagaata
      421 ccggcaccac cattcttgat aaaaatgggg tagtactcta taagggttat ggcgccgttg
      481 atcgacgatc gataccgctt ttcgagatgc ccccgtcggt agtacaagca acgctagcgg
      541 ctgaagaccc tgatttctac aaacaccccg gtgtttcgtg gcgaagcgta gctcgagccg
      601 cttaccgcaa tgccagcgag aatggcaaga ttcagggcgg atcgaccatc acgcaacaac
      661 ttgttaaaaa cacgctgctc accagtgagc ggtcgtttac ccgcaaatac aaagaaatgg
      721 ttctttcaat ggcaatggaa cgacgttata ccaaggatca aattctggag atgtatctta
      781 ataccattta ctatggccag ggcgcgtatg gcattcaaac agcagcccaa acctactttc
      841 acaaaccagc acgagaactc accttaggcg aaagtgcctt attggctggc ctgccgcaaa
      901 gtcctagccg atttgatcca aatatcaacg aagaaggagc caaaaaacgc cgcgattacg
      961 tattaagccg aatgcaagag ctgggatatg ccgagaatgt agcagtaagc gccgccaaat
     1021 tgcagccaat tgatgccggc agccatcaag ttgtactgaa agcacctcat ttcgtctttt
     1081 atgtgttgca gcagttacgc cgcgagtacg gcgaaaaaac cgttaaagaa ggggggatta
     1141 cggtaactac caccttggac tacatcaaac agcaagaggc tgagttgatt gttaaaaagc
     1201 agataaccaa gctcgtggcg cacaacagca ccaatggggc cctggtttca ctcaatccta
     1261 aaactggtga tatcatcagt atggttggca gcgtagacta taacgctccc ggttttggca
     1321 atgtaaatgt tacgctctcg gagctacaac cgggatcaag ctttaagccg attgcctatg
     1381 ccgtggcttt ttcgaaaggc tggaatggcg ctacggtggt tgatgataag ccggtgcgtt
     1441 ttcctcaacc tagcggagaa gtttttgctc cgcaaaacta tgatctcaaa ttccgaggcc
     1501 aggtattact acgccgagca ctggccaact cctttaatat tccagcgatc gaagtgctgc
     1561 agcacggcgg cctggaagca accctggcaa tggcgcataa tctgggtatt cgaccaccca
     1621 gtttaacgga aaccaaccgt tacggattaa gcttggtttt aggtggcggc gaggtgcggc
     1681 caattgatat ggcggcggtg tatgccacct tcgccaatgg cggtaaaaat gtcgcgcctc
     1741 aagctatcct cgaagtaaag gatcgtgctg gcaacagtat taccaagccg cggaccgtcc
     1801 cgacacgcca agccatcgac ccgcgcattg ctttcatgat cactaacata ttaagcgaca
     1861 ctgaggcgcg caaggaagag tttggcgcgc gcaatccctt acagctaagc cggcccgcag
     1921 ccgctaaaac cggtaccact aacgacttca gggacaactg gaccatcgga tatactccgc
     1981 agttggcgac cgccgtctgg gttggaaaca atgatcactc gccaatgctc aatgttgacg
     2041 gtatcaccgg agccgctcct atttggcatg attatatgga aatggcgttg gccgatagcc
     2101 cggtagagca gttcactccg ccatcgggtc tggcgttcac caaagtctgc ccgatcgatg
     2161 gcggattggc tgcccctaat gatccatcgg ctattgaaga ggtgtttctc gtggaaaacc
     2221 aaccaacaaa gcgctgcggg caaatccggc cgccagcagt tcttgtgcaa gctgtaactc
     2281 cccaaaatcc gactccgccg actccaggat ccccaagtga agctccgccc ttgctaccac
     2341 cggagccggg caggggagga ggtggtgacg acggagaccg cggcacgccg acacccgacc
     2401 caaaccaaca gcccgttagt ccgccgcctt caacatttcc ctttaaaaag cagtgaggga
     2461 aatattgaaa ataagcgttg acacacatct ggacttgttt tataatttat ttacctaact
     2521 agatatcgtg ttgtaataaa ataacgtaga agaagatgtc cgatactatt gtcccacaat
     2581 ctgcttttaa ctatgtccaa acggctgaag caattttggg cgcgctgcgt aatgagcgcg
     2641 accgccaaat tgttgctcaa cgctatggct taggcttgcc gaaacgccat accctggaaa
     2701 aaatcggcag ccagtttggt attacccgag aacgggtgcg tcaaattgaa aaagccgcca
     2761 cggttaaact gcaacagacc gcttctactg aactaacacg tatcgacaat gctcttaagc
     2821 agcatttagc ccggaacggt caggtcgacc ttcttcaaga cgtggccgca gctgtgggtg
     2881 cacacacccc ggcccagcag gcatatatct ccttcttggc cgccctggca cccagcattg
     2941 aaattattga agacagcgac cactatcatt ccgctctgtc caccttgccg gaataccaca
     3001 aacagcacat tgctgaaatt accagccatc tggttcacca cctcaagcaa aaggctaagc
     3061 ctaccccaat cgatgccctc cataaggcaa ttccatacga catcagcaag caaaccaccc
     3121 aacaggttgc tcgggtttct aaacgcttgg ctcatcatga cggccattgg ggtttgagcc
     3181 actggccgga agttaatccg cgctcaattc gcgataagac ctatttggtg ctgggcaagc
     3241 atggccagcc gcttcacttt accgatatcg ctacccatat cgccggcctc ggcaatcaaa
     3301 agcgcagtgt aacgactcag gcagttcaca acgaattaat caaggatccg cgctttgtac
     3361 tggtagggcg ggggatttat gctttaagcg aatggggcta cactcctggc accgtcgccg
     3421 acatcattac cgaagtcttg cgcgatgaat cgccgctcca taaagatgaa atcgttaagc
     3481 gcgtactgca aaaacgccaa gttaaaacca cgaccatcgt cctcaacctg caggaaaaag
     3541 atcagtttat ccgggtttcc aaggcgacct acgccttaaa ataagcaacg gatcagacgt
     3601 tagtacacag ggaagtaata aggtaaaata gaggcatggg gtgcacgctc attacgctta
     3661 gttcagccag cttagccggg aacaggttat ggattttcta ataactattc tgtttgcggt
     3721 tatgcagatc attggaacga ttctttttac ctactacggc tgggtgatcg tggtcgctat
     3781 ttttctctat cttatttggc aaaatcgacg ccgcgaacag tgggtcggcc agattgaaca
     3841 catcttattg cagatagaag ttcccaagga aaacgagaaa aaggagcttt cggccgagca
     3901 aatgttcgcc agcctacacg gcattttgcg ctcaaaatcc gatttgcgaa aggaaggctc
     3961 actgcaagaa catatcagtt ttgaaatcgc ggttattaat gaccagatcc gcttctatgt
     4021 ttggaccccg cgccatctta aaaattttgt tgaaggccaa atttatgccc agtatcccaa
     4081 tgtccaaatt actgagggca tagccgacta cgctaaaact gagctgggtt cacggcaagc
     4141 ctacactacg gttctgggtt tgaccaaaac cgaactactg ccgattaaaa ccttccaatc
     4201 atttgaagtc gacccgctag ccggcattac cgccaccctc gccaacatgg aagacccagg
     4261 cgaagaaatg tggattcaaa ttttagcccg ccccattgat gacgattggc acagtaaagg
     4321 gctcaattat attgaagata tcaaggaagg gaagacccac gaaccgttcg aacaaatgat
     4381 ttttaaggcg ttgctggcaa ttcccggcta cattttaacc aacttcttag gtgctctggt
     4441 tacacctccc ggggaagcta aagaagttaa aaaagagctg aaggccacaa ccggcgatca
     4501 agcgctggct aaagccgctg aggaaaagat tagcaagctg ggctaccagg tatatattcg
     4561 tatcgtttac ctgggaaatg acccaattgt tgcccggcag cgtaatcaag ccattattgg
     4621 cgggtttaag caatttaata ccaccaattt aaacggtttc tatgctgaat cgtcgaaaga
     4681 ggggacgggg ggggttgatg cgtaccgcgc ccgcctattt accagctccg gctatatctt
     4741 aaatattgag gagctggcca gcgtttatca cttgcctcat aaaagcgtag aaacgccgaa
     4801 catggtgtgg actactacta aaacatcgga gccgccgcca aacctgccaa ccgagctgac
     4861 cgccgagcca accggcatca gcttgtttgc tgccaccaac ttccgcggta agcagctcaa
     4921 gtttggcatg aagcggatcg accgcggacg ccacctctac atcattggtc aaaccggaac
     4981 cggaaaatcc aagctgctta acttgctaag cttgtcggat atttatcatg gagagggaat
     5041 ggcaattatc gacccgcacg gtgactttgc cactgacatg ctgcaatacg ttccggaaca
     5101 ccggctgcag gacgttattt acttcaaccc aaccgaccgc gattttccga tcgcctttaa
     5161 ccccctcgaa gtctacgacc aagcttttaa aaatgatatt tcctcggaaa tgatcggcgt
     5221 gctcaaacgg atgttcgaat catggggacc gcggctggaa tatatcttgc gctttacact
     5281 gctcgcactg cttgattacc cggacgccac aatgcttgga atcactcgca tgctaaccga
     5341 taaggaattc cgcaaaaaag ttattaagga aattaaagat ccggtcgtga aagccttctg
     5401 ggtaaccgag tttgcgtctt ggaacgataa gtttgccaac gaagcggtgg cgccggtact
     5461 caacaaggtg ggagccttca ccgccaaccc gctgattcgt aatattattg gtcaaccccg
     5521 ctcgcggcta gacttgcgca aaattatgga cgaaggcaag attttagtag ttaacctttc
     5581 ccgcggtcaa ataggggagg ataacgccag cattttagga gccttaatgg ttacgaagat
     5641 ccagctggca gcaatgtcgc gggcaaatat ccctttggat cagcgtcgcc cgttctacct
     5701 gtatgtcgac gaattccaga acttcgccac cgacagcttt gcaaccattc tcagcgaggc
     5761 gcgtaaatat gctctgaacc taacggttgc caaccaatac gtctcccaga tgccagaaga
     5821 ggtccgcagt gccgtcttcg gtaacgtcgg ttcgattgta agcttccgcg tgggtgtcga
     5881 cgatgcacca attattgcca agaactttgc gccgctgttt gagcccgatg acctaggcaa
     5941 gctgcataat cagcatattc taattgatat gagtattgat ggtgaaaagt cgatgccgtt
     6001 ctcggcaaaa accctgcgca tgcccgatcc cgagtacgac ttcacccctc aacttattga
     6061 actaagccgc cagcactatg cctctgatcg tacacaagtc gaacaagaga tttctgaatg
     6121 gacattaggt caggctgcta ccgaaaaacc cagcgccgag caggaagcca cacccgcgcc
     6181 tactcctcac gaacaaaaac cgaacagctt tttaggggcg ctgaaaaacc ccgaaccgcc
     6241 accagcgagc caaccggtac atgcagccgc gcataatccg ggatcaacag ggggcaacag
     6301 aaataaacga cgaggccgaa gcggtcagcg caaccaaaat gaacagatgg gcaacaggag
     6361 tggccagcaa gcgtcggtag gtgccggaac accggttcgt atggaaactc gcgcccacga
     6421 acgggcggtg cggccggaag agagtgtggt aatgcgggga ccagccggag aataagaaaa
     6481 ctcaggaaac ccagcagacg tgggcggtca attgcatctt gatcgaaagt ataaagctat
     6541 gtcgcacaat gtatattttg cgacatatag tatattaaaa agagttggta tgagtggggg
     6601 cttggtaccg ttgatttagg gtcaattatc ccttatataa gtttaaactt taccttcctt
     6661 cttttgtata cgcaccggct tttcgcttat taaggatgtt gtaacttttg cttttagggg
     6721 tacctaggaa cggtttatca gccatagctg cctgcccctc tccccttgcc aattatagct
     6781 tccgctggca gcgcctaaga acccgggatt tcccggtgct tattagacat tgattttagt
     6841 gtacaaaaac cgaacagagg agggaagagt ggaggagttc cgttaacagt aatcgggcga
     6901 ctgctctcct atttataata ggctttatga ggaatgcgaa gatcaatgta ctgcgtaggc
     6961 tgttttctcg tccgctttaa ttcaagttgg acttgcttga ggtcggctat ctcctccccc
//
LOCUS       DASVGB010000061         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_39141, whole genome shotgun sequence.
ACCESSION   DASVGB010000061 DASVGB010000000
VERSION     DASVGB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39141"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VF272_01760"
     CDS             <1..1133
                     /locus_tag="VF272_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7259635.1"
                     /translation="LDYRVVLDLHDASGNKINGTMDPFEEVWRTGKSLINNELTMQTK
                     GGHIVAISLMLSPIYDDNHKVAGGIALFHDISSDKEVERQRNEFISTASHEMRTPVAA
                     IEGYLSLAMNPAVATVDDRAKAYLEKAHTSTQHLGALFRDLLSVTKMEDSNRQDKQEI
                     FDLSQLTSEAVADMQFTAQKKGLEVQFSSADPIVRTQNTVMPVYAVKGNPQRIREVIT
                     NLMENAIKFTSGGVINVSIGGTTDTVTVSVSDTGIGIAAEDIPHLFQKFYRIDSTATR
                     TIGGTGLGLYLCRSIIERVGGRIWVESKLNEGSSFKFSLPRLASDKIMAQDAPTPESA
                     APNAAPTLAPAPVMAAATPQPGESAKIPTVTDISKPKPAAAV"
     gene            1183..1647
                     /locus_tag="VF272_01765"
     CDS             1183..1647
                     /locus_tag="VF272_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7259636.1"
                     /translation="MTKLMLVEDDFSLRDIYSARFMAEGYTVVTASDGEEALAIAVRE
                     KPDLIVLDVMMPKISGFDVLDILRSTPETKSTKVVMMTALSQETDRQRGESLGVDEYL
                     VKSQVTLEDVVSTVKGVLGTTANGGAPATGAQPLAPGQQQQPPPPAPPTPGV"
     gene            1662..2318
                     /locus_tag="VF272_01770"
     CDS             1662..2318
                     /locus_tag="VF272_01770"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7259637.1"
                     /translation="MRINRYVAAATGLSRRAADEVISQSRIHVNEHPAQLGMSVGPAD
                     SVRLDGTLLRVPQSQTIMLHKPVGYITSRRQQGQTPTIYRLLPPELRRLKPIGRLDRD
                     SSGLLLLTDDGELAQQLQHPSKGKWKRYEVRLDRPLGPEDQRQLEQGVTLPDGPSLLK
                     IQSQGSTHIVSLQEGRNRQIRRSFDALGYKVTSLHRTDFGSYRLGTLGEGEWKHESTA
                     "
     gene            2302..>3132
                     /locus_tag="VF272_01775"
     CDS             2302..>3132
                     /locus_tag="VF272_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7259638.1"
                     /translation="MNLLLNIVSLLIALAPLPALNAHVVEQLESRTYPSPVSETMPDY
                     QASTIPIKVVTVPLQLTASSAYAIDLDSNQTLYSSRSEVERPIASITKIVTALVILES
                     HNPSEIITIPTLPAYSLGDELSGFQPGMQLSINDMLTALLVQSGNDAADALAIIDSGS
                     REAFANKMNRFVDRWSITGAHFSNPSGLVETGNGASAEALAKIGSLALRNPTVTRLIK
                     MPNATITAKDGRALRVTTTNQLLLSGRFSGIKTGYTLVAGQCFIGLTTIQGHQVITVI
                     L"
BASE COUNT          832 a          815 c          791 g          694 t
ORIGIN      
        1 atttggatta ccgggtggtg ctggacctcc acgatgccag cggcaataag attaacggga
       61 ccatggatcc atttgaagaa gtttggcgga ccggcaaaag cctaatcaat aatgagctga
      121 cgatgcaaac caaaggcggc cacattgtgg ccatctcgct gatgctctcg ccgatttacg
      181 acgataacca taaagttgcg ggcggcattg cgctgtttca tgatattagc agcgacaagg
      241 aagttgaaag gcagcgcaac gagttcattt cgaccgcttc gcacgaaatg cgcacgccgg
      301 tagcggccat tgaaggctat ctttcattag caatgaaccc tgcggttgcc accgttgacg
      361 accgggcaaa ggcctatctg gaaaaagccc acacctcgac ccagcatctt ggcgcgcttt
      421 ttcgcgacct gctttcagtt actaaaatgg aagatagtaa ccgtcaggat aaacaagaaa
      481 tattcgacct ttcgcagctt accagtgaag cggttgccga tatgcagttt accgctcaaa
      541 agaaaggact ggaggttcaa ttctcatcag ccgacccgat tgtacgcact cagaatacgg
      601 ttatgccggt ttacgccgtg aaaggcaatc ctcaacggat tcgcgaggtc attaccaact
      661 tgatggaaaa tgccattaaa tttactagtg gaggcgtaat taatgtatcc atcggtggaa
      721 ccaccgatac cgtgaccgta agcgtcagtg acaccggtat tggcatagcc gccgaagata
      781 ttccacacct atttcaaaaa ttttacagaa ttgacagcac ggccacccga acaattggag
      841 gaaccggact tggattgtat ttgtgccgga gtattattga gcgggtagga ggacgcatct
      901 gggtagaaag caaactaaat gaaggcagca gctttaaatt tagtttgccg cgactggcga
      961 gcgataagat aatggcgcaa gatgcgccga ctccggaatc agccgcaccg aatgcagccc
     1021 ccacgctggc acctgcgccg gtaatggctg ctgccacacc gcaacccgga gagtctgcta
     1081 aaattcccac cgttaccgat atttcaaaac ctaagccggc ggctgcggta tgattgctcc
     1141 agatcgtatt gcctgttatg cttatggtaa gaggagcctg ttgtgaccaa gttaatgtta
     1201 gtagaggacg atttcagcct acgcgatatc tatagcgccc gctttatggc agagggctac
     1261 accgtagtaa cggcttcgga cggcgaggaa gcgctagcaa ttgcggtaag agaaaagcca
     1321 gacttaattg ttttggatgt tatgatgccc aaaattagcg gttttgatgt attggatatt
     1381 ctgcggtcaa ccccggaaac caagtcaaca aaggtagtta tgatgactgc gttgtcgcag
     1441 gaaaccgatc gacaacgcgg ggaaagccta ggcgttgatg aatacttggt taaatctcaa
     1501 gttacccttg aagacgtggt gagtacggtt aaaggagtgc tcggcactac tgccaatggt
     1561 ggtgctccgg ctactggtgc gcaacctctc gcacctggcc agcagcagca accgccgccg
     1621 ccagcaccgc caacccctgg agtctaaggc ttaccgctga catgcgcatt aaccgttatg
     1681 tagcagccgc caccggcctt tcgcgccgag ctgccgatga ggttatctcc cagtctcgga
     1741 ttcacgtgaa tgaacaccct gcccagctag gcatgagtgt cggccctgcc gatagtgtac
     1801 gtttggacgg cacgttgcta cgcgtaccgc aaagccaaac aatcatgttg cataaaccgg
     1861 tcgggtatat tactagccga cgacagcaag gccaaacccc aaccatctac cgcttgctac
     1921 ctccagagtt acggcgttta aaaccaatag gacggctcga tcgcgacagc tccggattac
     1981 tgctcttaac cgacgatggg gagttggcac agcagcttca acaccccagc aagggcaaat
     2041 ggaagcgata cgaggtacgc ctggaccggc cattgggtcc cgaagaccag cgacagctgg
     2101 agcaaggagt aaccttgccc gacggcccca gtctcctgaa aatacagagc caaggatcaa
     2161 cccatatcgt gagcctccaa gagggacgaa accggcaaat ccggcgcagc tttgacgcct
     2221 tgggttacaa agtaaccagc ttgcaccgca ccgattttgg ctcatatagg cttggtacac
     2281 tgggggaggg agaatggaag catgaatcta ctgcttaata ttgttagctt gctgatagct
     2341 ctagcgccgc tacctgcctt gaatgctcat gttgttgaac agcttgaaag ccgaacctat
     2401 ccatcaccgg ttagtgagac aatgccggac taccaagcat ccactattcc aataaaggta
     2461 gtaacggttc cattgcaatt aacggcaagc agtgcctatg cgattgattt ggacagcaat
     2521 caaacgctct atagttctcg ttctgaggtg gagcggccaa tcgcctccat aaccaaaatt
     2581 gtaaccgccc tggtaatctt agaatcacac aatcccagcg agattattac cattccgaca
     2641 cttccagcct acagcctcgg tgacgagtta agcggatttc aacccgggat gcagctcagt
     2701 atcaacgaca tgctcaccgc tttattggtg caatcaggca acgacgcagc cgatgccttg
     2761 gcaattattg acagtggaag tcgggaagct tttgcgaata aaatgaaccg gtttgtagac
     2821 cgatggagca tcaccggcgc acacttttcc aacccgtcag gcttggttga aacgggtaac
     2881 ggcgcttcgg ctgaagcttt agctaaaatc ggctcgctcg ccttacgtaa tccaaccgta
     2941 acccgtctca taaaaatgcc aaacgctacc atcacagcga aagatggaag ggcattacgc
     3001 gtaacgacca ccaaccagct tttgctttct ggtaggtttt ctggtataaa gacgggatac
     3061 accttggtgg ccggccagtg ctttattgga ttaaccacta tccaaggcca ccaagttatc
     3121 accgtcatac tc
//
LOCUS       DASVGB010000062         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_396770, whole genome shotgun sequence.
ACCESSION   DASVGB010000062 DASVGB010000000
VERSION     DASVGB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396770"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VF272_01780"
     CDS             complement(<1..483)
                     /locus_tag="VF272_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7259639.1"
                     /translation="MSAKSQILIPTVLEKTAYGERAYDIYSRLLKERILFIGTPIDDN
                     VANLTIAQLLFLQSEDPKKDVQMYINSPGGSVSAGLAIYDTMRHIKPDVSTIVMGLAA
                     SFAALLSTAGAKGKRFALPNAQMMIHQPWSEGVGGQATDIDIRAREILKTRDRLNKIL
                     S"
     gene            complement(550..1827)
                     /gene="tig"
                     /locus_tag="VF272_01785"
     CDS             complement(550..1827)
                     /gene="tig"
                     /locus_tag="VF272_01785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7259640.1"
                     /translation="MKAEVTKRTDTHISFMITADSGELQHAKEHAYDKYRPDVKVAGF
                     RPGKAPDSIVAREVGDTAIQQEALEHAIGHSYSAAVAAEKVAVIARPDIQVKKFVPYT
                     ELEYEATVEIVPPVTLPDYKKIKKPAKSVSIEEKQVEEMLEDLRRRVAKRIPAIRGSE
                     MGDEVKFDYDGVMKGEPVPGASGKNYTLKLGSGNFIPGFEEELVGLKPGEEKTFTITF
                     PKDYHEKSLAGQPVVFTAKINEVTELMLPEVNDSFAAEVGPFKTVQALRTDLKDQLKA
                     EAEQAAQREYENELLDELIAKSKMTVPERLLAQQLERLKAEISQRLASSGLTMEHYLS
                     AQKQTMEELEKQMRPEAEKRVKLALMLSEIARQEKLSVGADEIENEIEGLRLQYTDPA
                     MQKELAGDQIKEDVYNHLMAGKVIKSLTTYAQS"
     gene            complement(1879..>2233)
                     /locus_tag="VF272_01790"
     CDS             complement(1879..>2233)
                     /locus_tag="VF272_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7259641.1"
                     /translation="YYFPSKQEFQQLWEVNSIDEGLHRMKGLTSALTIVKDSDNGALA
                     MKGDEVLRIPAFPVNPIHTVGAGDSFNAGFMVAQALGRDLEKSMRFGCAAAGLTISQP
                     TMPAIEAVETLAASA"
BASE COUNT          502 a          596 c          500 g          635 t
ORIGIN      
        1 ggagagtatt ttgtttaacc gatcacgggt ttttaatatt tcacgggctc gaatatcaat
       61 gtcagttgcc tgtccgccca ctccttcact ccacggttga tggatcatca tttgggcatt
      121 cggcagagca aaccgcttac ctttagcccc ggcggtactc aaaagggcgg caaacgatgc
      181 ggccagcccc atcacgatag ttgagacatc tggtttaatg tggcgcatgg tgtcgtagat
      241 ggccagcccg gcgctgacac ttccgcccgg tgagtttatg tacatttgaa catccttctt
      301 gggatcttca ctttgcaaaa atagcagctg tgcaatggtc aagttggcta cattgtcgtc
      361 aatgggtgtt ccaataaaca aaatgcgctc tttcagcagc cgtgaataaa tatcataagc
      421 gcgctcgcca taagcggttt tttccaacac ggtcggaatt aaaatttggc ttttagcaga
      481 cattgcagct ccttcggcat ttagattcca ataatactaa attggcagtc gcatgtctag
      541 agtgctagct taggattggg cgtacgttgt caggctcttt ataactttgc ctgccatcaa
      601 atgattatag acgtcttcct tgatctgatc gccagccagc tctttctgca ttgccggatc
      661 ggtgtactgc aagcgcagtc cttcgatttc attctctatt tcatcagcac ctaccgatag
      721 tttttcttgg cgggcaatct cgctgagcat caaggccagt ttcacgcgct tttcagcttc
      781 ggggcgcatc tgcttttcca gctcttccat ggtctgcttt tgagcagata gatagtgttc
      841 catagtcagt ccacttgatg cgagacgctg actgatttcg gctttcaacc gttctaactg
      901 ctgagccaac agccgctctg gtacggtcat cttgcttttt gcgataagtt catcgagcag
      961 ttcgttttcg tactcccgct gtgccgcttg ctcggcttcg gccttaagtt gatccttaag
     1021 atcagtccgc aatgcttgga cagtcttgaa aggtcccacc tctgcagcaa aagaatcatt
     1081 aacctctggc agcatcaatt cggtcacttc attgatctta gcggtaaaga caactggctg
     1141 gccggccaag cttttttcgt ggtaatcctt cgggaaggtt atggtaaatg tcttttcctc
     1201 accgggctta agacccacca gctcttcctc aaatcctggg ataaaatttc ctgatcctag
     1261 cttgagtgta tagtttttac ccgaggctcc aggaaccggt tcacctttca taacgccatc
     1321 gtaatcaaat tttacctcgt cgcccatttc cgaaccacga attgccggta ttcgcttggc
     1381 tactcgccga cgaagatctt caagcatctc ctccacctgt ttttcctcaa tcgaaaccga
     1441 cttggctggt tttttaatct tcttgtaatc gggaagcgta accggtggta cgatttcaac
     1501 cgttgcctca tactccagct cggtataggg cacaaacttt ttaacttgaa tatcaggtcg
     1561 cgcgataacc gccacctttt cggcagctac tgccgctgag taactatggc cgatggcatg
     1621 ctccaatgct tcttgctgaa tcgcagtatc gccaacttca cgagccacaa tactatcggg
     1681 cgccttgccc ggacgaaaac cggcaacctt aacgtctggc cggtacttat cgtacgcatg
     1741 ctccttagcg tgttgcaatt cgccgctatc ggccgtaatc ataaaactaa tgtgcgtgtc
     1801 ggtacgcttg gtaacttcgg ctttcataac ccgagtattg tagcagagga gtatagggga
     1861 tgccagcccg atgattttct aggcgctagc agctaaagtt tcaaccgcct ctatcgcggg
     1921 catcgtcggt tgggagattg ttaatccagc agccgcacag ccaaaacgca tgcttttttc
     1981 caagtcccgc ccgagggctt gagcaaccat aaagccggca ttaaaactgt cgcccgcacc
     2041 cacggtatgt atcggattta ccggaaaggc gggtatgcgc agtacttcat cgcccttcat
     2101 cgccaaggcg ccattatcgc tgtcctttac tatggtaagt gccgaagtca gtcctttcat
     2161 ccgatgaagc ccttcatcaa tgctattcac ctcccacaac tgctgaaatt cctgcttgct
     2221 tggaaaataa taa
//
LOCUS       DASVGB010000063         8434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_73784, whole genome shotgun sequence.
ACCESSION   DASVGB010000063 DASVGB010000000
VERSION     DASVGB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73784"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2544)
                     /gene="leuS"
                     /locus_tag="VF272_01795"
     CDS             complement(67..2544)
                     /gene="leuS"
                     /locus_tag="VF272_01795"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7259642.1"
                     /translation="MKRYEPKQLEPKWQQIWEDTKLYEVGEEPSRQKAYVIDMFPYPS
                     GAGLHVGHVRNFSISDTYALYLRQKGKNVLRTIGFDAFGLPTENYAIKTGISPQEATD
                     RQIAVFKGQLKKLGMSYDWSREAVTSDPGYYRWTQWIFLQLLKSGLAYQKENLQWWCE
                     HDQTVLADEQVVNGRCWRCDNPVIKKSLKQWFFKITDYADRLLEGLNELDWPEKIKIQ
                     QRNWIGRSVGAELRFEIEGHADEQIDVFTTRADTIFSAAFIVLAPEHALVEKITTQAQ
                     QKDIAQYIEESAGKDDVERLAEGDKEKTGVFTGSYAINPANEEKLPIWIGDFVLGGYG
                     TGAVFGDIHDERDFAFLNKFDIPARVTVIPEDKDEAVKVQAKEYTYTGEGILIDSGEF
                     TGLRSEVARDQITAWLAAKGSAKETVNYKMRDWLISRQRYWGAPIPIIHCPKDGAVLV
                     PEDQLPVELPKVESYQPNGDGRSALARAKDWLHTTCPECGGPAERETDTMDGYACSSW
                     YFLRYTDPHNDKEAFSRAKADYWMPVDIYVGADHAVAHLLYARFWTMFFRDQDLLTVE
                     EPFRGLRYNGYILAPDGSKMSKSKGNVVDPVDLIEEGYGADALRLYELFIGPYDQNIN
                     WNPTGIDGTKRFLNRVWALVQEHLEAKPDQGNTGTDQALETAVAVAVHKTIKKVTVDL
                     EKFGFNTAIAAQMELVNELYRLKTKLPLGSEVWQKNLRLVVAIVAPFAPHISEELWEQ
                     LDGKGSVHEASWPTFDPTLVEDALMTIVLQVNGKTRGQIELNSDVAEDEVKQMAAESD
                     AVKRHSTGLTIVKTIYIPGKLVNFVVK"
     gene            2605..3696
                     /locus_tag="VF272_01800"
     CDS             2605..3696
                     /locus_tag="VF272_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7259643.1"
                     /translation="MSQPNPKRFSSPASFIGLQNQGIISFGSGQPDLPPPPGIFEKLE
                     TPQVFQYGLIQGEIELRERLSQDYPGIRPDNYIITNGASEALDLTFRAIHELHGPVKV
                     LVCRPYYYSYPPILELAGLEPVYSDLVDGRIDLEDFKTKLPEVKAILINSPSNPTGRI
                     EDIETLKTIEAIAAEHDKYVISDEVYKDLIYTRENYYIKGKHVITINSFSKTYAMCGL
                     RIGYLCTPDHALLDKVFEMKTHTSMNTSIIGQQMALRALNVPPGYVEDQTAVWKERRD
                     FIYNELVAMGFDLWNPEGAFYVFPRVSNASQVVTDLFYDHQVIVYDGAWFGDPGRIRL
                     SYALDIKQMEEGLKRIGHYFEAKKDWLSN"
     gene            complement(3697..4722)
                     /locus_tag="VF272_01805"
     CDS             complement(3697..4722)
                     /locus_tag="VF272_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX7259644.1"
                     /translation="MAQIEVKPIDNQQDWGAFLASHPESNFLQSWQWGEFHLALGKTV
                     LRRGFYKSGKLVGLMLSVVEPARRGRYVTVPGGPIIDWHDTAAVKVFVAEIRVQAKTE
                     KCVFVRVRPQLITDDFSTTLFAELGFRGAPTHLHAELTQQLDITLPEEEILTKMRKGT
                     KYEVKNSIKVGINVTSSAETKDMQAFYDLQIETAKRQGFVPFSYQFLEKQFQVFTAAK
                     QALLYTAAYEGQVLAQAFIIFQGQEAVYHYGASTDAGRQYPGAYAIQWEAIKEAKRRG
                     MSRYNFWGIAPPDQPNHRFAGITRFKRGFGGEAVEYLHARDLVINQAYYLVNLVIEET
                     RRRMRRV"
     gene            complement(4704..5903)
                     /locus_tag="VF272_01810"
     CDS             complement(4704..5903)
                     /locus_tag="VF272_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7259645.1"
                     /translation="MSVFNSLGSNYRPSAVMQALSGIHQPGAAQKLTALLEERYGGHA
                     TLVYKGREALCLAFKTLDLPKGSAVAISGFTCFAVYKAVESAGLEAHFMDIEPEQLNF
                     SAATLAAAVESNPAIKAVVIQNTLGYACDIQGIVEVCRKHGIMLIEDLAHSPGIVYAD
                     GGEAGTVGDFVMLSFGRDKMIDAVSGGALVARNPKYQLKSSSRLPKPPLTPRFRDHLY
                     PLLTAKIRFGYGFGIGKLVHAAYKALGLLSRSVDGEFYQGHRLPGWYCALTLKGFSTL
                     ESNLARRQRIAQVYAQTLQRGVQFLELPSQTPQASNLRFPIRVRHRDELIQYLAQQQI
                     FIADTWYDAPIAPPRYIEKTTYQKGQCPGSEQVVAHILNLPTHRAISESDALNISEHI
                     NAWLKSK"
     gene            complement(5900..6598)
                     /locus_tag="VF272_01815"
     CDS             complement(5900..6598)
                     /locus_tag="VF272_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7259646.1"
                     /translation="MGALGKIYILHGWTYSTKRWEPLVAWLKQQGFEPEMLHVPGLTA
                     ESRDVWDVPKYVEWLNQATAKHPKPFVLMGHSNGGRIGLAFAARYPKRIHHLILVDSA
                     GIYHNEPLLQLKRLVFGKVAKAGKALTSSAKIRAALYKIARAQDYHQAPPNMRTTMIN
                     MHEADKLLPVEQIFAPTTIIWGREDRVTPLADGQALEQRLPHAKLHIIEGGRHTPVYT
                     HHEEVGQIIVGALK"
     gene            complement(6586..7857)
                     /locus_tag="VF272_01820"
     CDS             complement(6586..7857)
                     /locus_tag="VF272_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX7259647.1"
                     /translation="MIQKLKQILYFPVAGYFRFWARLQFNRWNPRVVLVTGSSGKTTL
                     LHMLESQIGKRAHYSHHANSAFGIPFDILGFPPRSNAKSEWIDLFITAPFRAFKKPLN
                     ESIYVVEADSDRPGEAEFISSLVKPEVTIWLSSDKSHSYNFDSQVADGLFLDVREAIA
                     YEFGAYLAAAAKLAIINDSEALIINQGDRTSAKIEAVRPDTITGYAIKADHTVFKIDG
                     TTYTVPGLVPQEAGLSMAAVVEACRYLGIEVDKKFSSFIAPPGRSTVFKGIKKTTLID
                     STYNAIPDAVRAVLGLFAKYPGKPKWLVISDMLEQGDSEADEHRAIAESIAKLNLDRI
                     ILMGPRMSKYTAPRLKELLGDKAPIEVFLKPRPVLDYLQNHLKGGEIILFKGSRFLDG
                     VVEHLLSDPSDEAKLCRRGPVWQRRREQWEL"
     gene            complement(7892..>8434)
                     /locus_tag="VF272_01825"
     CDS             complement(7892..>8434)
                     /locus_tag="VF272_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259648.1"
                     /translation="IAAFAKGLVDQGWQVLASGGTAKILAAAGISVRDVAELVGGEAI
                     LGHKVVTLSREIHAGLLAGYETDKDEMERLGLSYIDLVCVDLYPLEQEQDNLDRTLES
                     IRQKTDVGGPTMLHAAAKGGRLIVCDPDDRQGVLDWLSQGSPNEAKKRLAMAIKAEMV
                     AAHHIELSEQLLTEELTKQK"
BASE COUNT         2091 a         2336 c         1916 g         2091 t
ORIGIN      
        1 tgtggcagac cgatccggag ctatatctat tttatattga aacgggtttt atccgctaag
       61 tcccgcttat ttaaccacga agttgaccaa cttacccgga atgtaaatag tcttcacgat
      121 tgtcaagccc gtggagtggc gcttcacggc atcactttcg gctgccattt gcttcacctc
      181 atcctcggcc acatcggaat taagctcgat ttgtccgcga gtcttaccgt ttacttgcag
      241 cacaatcgtc ataagagcat cttccacaag tgtcggatcg aacgttggcc agctggcctc
      301 atgcacgctg cccttgccat caagctgctc ccataactcc tccgagatat gcggtgcaaa
      361 cggcgccaca atcgcaacca ctaagcgcag atttttctgc catacctcgc tgcccaacgg
      421 caacttagtt ttcagccggt acagttcatt caccaactcc atctgagcag caatcgcggt
      481 attaaaacca aacttttcaa gatcaacagt aactttctta atagttttat gaacggcaac
      541 tgccacggcc gtctccaatg cctgatcggt tccggtattg ccttgatccg gtttagcctc
      601 caggtgttct tggacgagcg cccacacccg gttcaaaaag cgcttagtgc cgtcaatacc
      661 ggtaggattc cagttaatgt tttgatcgta gggaccaata aataattcat acagtcgtag
      721 agcgtcggcc ccataccctt cctcaatcaa atcaaccgga tcaaccacgt ttccctttga
      781 tttactcatt ttcgatccgt caggagccaa aatataaccg ttgtagcgca ggcccctgaa
      841 aggctcttca accgttagta aatcttgatc acggaaaaac atcgtccaaa aacgagcata
      901 caacaaatgg gcaaccgcat gatcggcgcc aacataaata tccaccggca tccaataatc
      961 cgcctttgcg cgggaaaagg cctctttgtc attgtgggga tcggtataac gcaggaagta
     1021 ccagctggag caggcatagc catccatagt atcggtttcc ctttcggccg gaccgccgca
     1081 ctccggacag gtcgtgtgca accaatcttt agcccgggct aaagccgaac gcccgtcgcc
     1141 gttgggctga tacgactcca ccttgggcag ctcgaccggc aactggtctt ccggcaccag
     1201 cacagcgccg tccttcgggc aatgaatgat cgggattggc gcaccccaat accgttggcg
     1261 gctgatcaac cagtcgcgca tcttgtaatt aacggtttcc ttcgccgagc ccttagctgc
     1321 caaccaagca gtaatttggt ctctggccac ctcgctgcgc agaccggtaa actcaccgga
     1381 atcaattaaa atgccttcac cggtataggt atattccttg gcctggacct taactgcctc
     1441 atccttgtcc tctgggatta ccgtcacccg cgccggaata tcaaacttgt ttaagaatgc
     1501 aaaatcgcgc tcatcgtgga tgtcgccaaa gactgcaccg gtaccgtagc cacccaatac
     1561 aaagtcacca atccaaatcg gaagtttctc ctcattagcc ggattaatgg cataggatcc
     1621 ggtaaaaaca cctgttttct ccttatcgcc ttcggctaac cgctccacgt cgtccttgcc
     1681 ggccgactct tcaatgtatt gtgcaatatc cttctgctga gcttgagtcg taattttttc
     1741 aaccaaggca tgctccggtg ctagcactat aaaggctgcg ctaaagatgg tatcagcgcg
     1801 cgttgtgaag acatcaatct gctcatccgc atgaccctct atctcaaacc taagctcagc
     1861 cccgacgctg cggccaatcc agttgcgctg ctgaatttta atcttctctg gccagtccaa
     1921 ttcattgagc ccctccagca aacgatcggc ataatcggta atcttaaaaa accactgctt
     1981 caatgacttt ttaatcactg ggttatcgca gcgccagcac cggccattca ccacctgctc
     2041 atcggccagc acagtttgat cgtgctcgca ccaccactgc aaattctctt tttggtaggc
     2101 caagccgctc ttcaataact gcaagaatat ccactgggtc cagcgataat accccggatc
     2161 gctggtaact gcctcacgcg accagtcgta tgacatgccg agctttttca gctgtccttt
     2221 aaaaacggca atctgcctgt cggtcgcctc ctgcggtgaa ataccggttt taatcgcata
     2281 gttttcggta ggcaaaccga aggcatcaaa accgatggtc cgcagcacat tcttcccctt
     2341 ttggcgcaga tacagcgcgt aggtgtcgct aatcgaaaag ttgcgtacat ggccgacgtg
     2401 caaaccggcg cccgaggggt atggaaacat atcgattacg taagctttct ggcgcgacgg
     2461 ttcctcgccg acttcataca gcttggtatc ctcccaaatc tgttgccatt tgggttcaag
     2521 ttgtttgggc tcgtaacgtt tcatgtatgg cttattgtag caaattgctt accgcttgag
     2581 cttaggtatg gcataatgag aggaatgtcg cagccaaacc ccaaacggtt ttccagccca
     2641 gccagcttta ttggccttca aaaccaagga attatttcct ttggatccgg acaacctgat
     2701 ctgccgcccc cgcccggtat ttttgagaag ctggaaacgc cgcaagtctt tcaatacggg
     2761 ttaattcaag gtgaaattga actgcgcgag cggcttagcc aggactaccc cggcatccgt
     2821 ccggataatt acattattac caacggcgca tctgaggctc tagacctgac cttccgtgcc
     2881 attcacgaac tccacggccc agtgaaggtt ctcgtctgcc ggccgtacta ctattcctac
     2941 cccccaatcc ttgagttggc cggtctggaa ccggtgtatt ccgatcttgt tgacggacga
     3001 atcgatcttg aagattttaa aaccaagcta ccggaagtca aagcaatcct catcaattcc
     3061 ccctcaaatc ctactgggcg cattgaagac attgagacac tcaaaaccat tgaagcaatc
     3121 gccgctgaac acgacaaata tgtgatttcg gatgaggttt ataaagattt aatttatacc
     3181 cgcgaaaact actacatcaa gggcaagcac gttattacca ttaactcctt ctccaagacc
     3241 tatgccatgt gtggcctgcg aattggctac ctctgcacgc ccgaccacgc ccttctcgac
     3301 aaagtatttg aaatgaagac ccacacttcc atgaatacca gcatcatcgg ccagcagatg
     3361 gcgctacgag cgctcaacgt ccctccgggc tacgttgaag accaaaccgc cgtctggaaa
     3421 gagcgccgcg actttattta taacgaactg gttgccatgg gtttcgactt atggaacccg
     3481 gaaggggcat tttacgtctt tcccagggtt agcaatgcct cgcaagtcgt tactgatctc
     3541 ttctatgatc atcaagttat tgtgtacgac ggcgcgtggt ttggtgatcc cggtcggatc
     3601 cggctatcat atgcactaga catcaagcag atggaagaag ggctgaagcg gatcgggcac
     3661 tattttgaag ctaaaaaaga ctggctcagc aattagttat acccgtcgca ttcgtcgccg
     3721 ggtttcctcg atcactaaat taaccagata gtacgcctga ttaatcacca ggtcgcgagc
     3781 atgcagatat tccaccgcct cgccgccgaa gccgcgctta aatcgagtga tgccagcaaa
     3841 gcggtgattc ggttggtcgg gcggagcaat cccccaaaag ttgtaacggc tcatcccccg
     3901 acgcttagcc tcttttatgg cctcccattg aatcgcgtag gcacctggat actgccggcc
     3961 ggcatcggtg ctggcgccat aatggtaaac cgcctcctgg ccttggaaaa taatgaaagc
     4021 ctgagccaaa acctggccct catacgcggc agtatagaga agtgcttgct tagcagccgt
     4081 aaagacctgg aactgctttt ccaaaaattg atacgaaaac ggtacaaatc cttgacgctt
     4141 agccgtctca atttgcagat cgtaaaatgc ctgcatgtcc ttggtttcgg ctgagctggt
     4201 aacgttaatg ccaaccttta tgctgttctt tacctcatat ttcgtaccct tgcgcatctt
     4261 agtcaaaatt tcttcttccg gtagcgtaat atctagctgc tgcgttagct cggcatgcaa
     4321 atgagtagga gcgccgcgaa atcccagttc agcaaaaagt gtagtagaaa aatcatcggt
     4381 tattaactgg gggcgaaccc gcacaaacac acatttttcg gttttcgctt gaacccgaat
     4441 ctctgcaacg aataccttaa ccgctgctgt atcatgccag tcaataatcg gtccgcccgg
     4501 aacggttacg tagcggccgc ggcgagcggg ctctacaacc gacagcatga ggccaactag
     4561 cttaccgctt ttatagaatc cgcggcgaag cactgtcttg ccaagcgcta aatgaaactc
     4621 gccccattgc caggattgca aaaaattgga ttccggatgg gacgctaaaa acgcccccca
     4681 atcctgctga ttatcgatcg gttttacttc gatttgagcc atgcgttgat atgctcgcta
     4741 atgttaagtg catcggactc ggaaattgcg cggtgagtag gtaaattgag aatgtgtgca
     4801 accacttgct cggaacccgg acattggccc ttttgatagg tagttttctc gatgtagcga
     4861 ggcggcgcaa tcggcgcgtc ataccaggta tcagcgatga aaatttgctg ttgagctaaa
     4921 tactgtatca gctcatcgcg gtgcctaact cgaattggaa agcgtaagtt cgaggcttgc
     4981 ggcgtttggc tgggtagttc tagaaactgc acgccgcgtt gtagcgtttg ggcatacact
     5041 tgtgcgattc gctggcggcg agccaaatta ctctcaagcg tgctaaagcc tttcagggtt
     5101 aaggcgcaat accatcccgg caaacgatgg ccttgataaa attcgccgtc aaccgacctt
     5161 gaaagcaggc caagtgcttt gtaggctgcg tgcactaact tgccaatgcc aaagccatag
     5221 ccaaatctga ttttagcggt tagtaatgga tataagtgat cgcgaaaacg cggagttagc
     5281 ggaggcttgg gcaaacgaga gctagactta agctggtact tgggattacg tgcaaccagg
     5341 gctccgcctg aaacagcatc gatcattttg tccctcccaa acgatagcat cacaaaatct
     5401 cctaccgtcc cggcttcgcc gccatcggca tagactatgc ccggactgtg cgctaaatct
     5461 tcaataagca taatgccatg cttacgacat acttcaacta ttccctgaat gtcgcaagca
     5521 tatcctaagg tattttgtat gaccacagcc ttaatggctg gattcgactc gactgccgct
     5581 gccaatgttg cggccgaaaa attgagttgt tccggctcga tatccataaa atgggcttca
     5641 agaccggcac tttcaacggc tttataaacc gcaaaacagg taaatccact aatggcaacc
     5701 gctgaacctt tgggaagatc gagcgtttta aaggccaggc aaagggcttc gcgcccctta
     5761 taaaccaggg ttgcatgacc cccataacgc tcctcaagta aagctgtaag cttttgggct
     5821 gctcccggct gatgaatgcc agacaacgcc tgcattaccg ccgagggcct gtagttggag
     5881 ccgagtgaat taaacacact catttgagcg ccccaacaat aatctgacca acctcttcat
     5941 ggtgggtata caccggcgta tgccgaccgc cttcgattat atgcagtttg gcgtgcggca
     6001 agcgttgttc taaggcttgg ccatcggcta gaggagtaac cctatcctcc cgcccccaaa
     6061 taatggtggt gggggcgaat atttgttcga ccggcaacaa cttatcggcc tcgtgcatat
     6121 taatcatcgt tgtgcgcata ttaggcggcg cttgatggta gtcttgtgcc cgagctatct
     6181 tatagagtgc tgctctgatc ttggctgaag aagtcagcgc tttaccggcc ttggccacct
     6241 tgccaaatac cagccgtttc agctgcaaca gtggttcatt atgataaata ccggcgctat
     6301 caactaaaat cagatggtgt atccgtttgg ggtagcgtgc ggcgaaggcc agtccgatgc
     6361 gacccccatt tgaatggccc atcagcacga aaggcttagg atgcttagcc gtcgcctgat
     6421 tcagccactc aacatatttg ggcacgtccc atacgtcgcg gctttcagcg gttaagcccg
     6481 gtacatgcag catttccggc tcgaaaccct gctgttttag ccaggcgacc aatggctccc
     6541 agcgtttggt cgaataggtc cagccatgca aaatgtaaat cttacctaaa gctcccactg
     6601 ttcacgcctc cgctgccaca ctggtccgcg ccggcaaagc ttcgcttcat cgctgggatc
     6661 ggacagcaaa tgctctacca caccatccaa aaaccgagat cctttaaaca aaatgatttc
     6721 acctccctta agatgatttt gcaggtaatc aagtaccggc cggggtttta aaaacacctc
     6781 aatcggtgct ttatcgccga gcagctcctt gagtcgcggc gcggtatatt ttgacatgcg
     6841 cggacccata agaataatgc gatcgagatt caactttgct atcgactctg caattgccct
     6901 atgctcatcc gcctccgaat cgccctgttc taacatgtcg ctaataacca gccacttggg
     6961 ctttcccggg tatttcgcga acaaaccgag taccgcccgc accgcatccg gaatggcatt
     7021 ataggtactg tcaatcaagg ttgtcttctt gatgccctta aaaaccgtgc tacgccctgg
     7081 cggcgcaata aagctcgaaa actttttatc aacctcaatg cccaagtagc ggcatgcctc
     7141 cacaacggca gccatcgaca aacccgcctc ttggggcact aagcccggta ctgtataggt
     7201 ggtaccgtca attttaaaga cggtatgatc ggctttaatt gcatacccgg taatagtatc
     7261 cggacggacc gcttcaattt ttgccgatgt acggtcgcct tgattgataa tcagggcttc
     7321 ggaatcatta ataattgcta atttagcggc tgcagccaga taggcgccga attcataggc
     7381 tattgcctct cttacatcca agaacaaacc atcggccacc tggctatcaa aattataact
     7441 atggctttta tcgctgctca accaaattgt cacctccggc ttcactaatg agctaataaa
     7501 ctctgcctcc cctggccggt cggaatctgc ctctactaca taaatggatt cgttgagggg
     7561 ttttttaaag gcgcgaaacg gggctgttat aaacaagtca atccactccg acttggcatt
     7621 gctgcgcggc ggaaaaccca aaatatcaaa aggtatgcca aatgcgctat tggcatgatg
     7681 cgaataatga gcccgcttac caatttgcga ctccagcata tgaagcaagg tagttttgcc
     7741 gctagaaccg gttaccagga caactcgggg attccatcgg ttaaactgca aacgggccca
     7801 aaaacgaaag tatccggcaa caggaaagta cagtatctgc ttcaatttct gaatcataac
     7861 ctaattgtag catcgctaca ggtgattttg cctacttctg ctttgtcagc tcctcggtaa
     7921 gcagttgctc ggatagttca atatgatgcg ctgccaccat ttcagcttta attgccatgg
     7981 ctaggcgctt cttggcttca ttgggcgaac cttggctgag ccaatcaagc actccctgcc
     8041 gatcatccgg atcacagaca attaaacggc ctccctttgc cgctgcatgc agcatagttg
     8101 gaccgcccac gtcagttttt tgacggatgc tttcaagtgt ccgatctaga ttgtcctgct
     8161 cttgttccaa cggatataaa tccacgcaca ccaggtcgat ataagaaagt cccagccgct
     8221 ccatttcgtc tttatcggtt tcatagcctg ccaataaccc ggcatgaatc tcgcgcgaca
     8281 acgtgacaac cttatggccc aaaattgcct cgccccctac caactctgct acgtcgcgga
     8341 cactaatccc ggcagcggct aagatcttcg cggtaccgcc ggaagccaaa acctgccagc
     8401 cctggtcaac caaccccttc gcaaacgctg cgat
//
LOCUS       DASVGB010000064         3734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_287311, whole genome shotgun sequence.
ACCESSION   DASVGB010000064 DASVGB010000000
VERSION     DASVGB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287311"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1074
                     /locus_tag="VF272_01830"
     CDS             139..1074
                     /locus_tag="VF272_01830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7259649.1"
                     /translation="MKAAQISEYGDASVITINEIDKPNVGKRQILVEVHASSINPFDA
                     KLRAGYMKERIPLQFPATLGGDIAGIVSERGDDVTTVVVGDQVYGQAAIVAGGSGAFA
                     EYAATKASQIAKAPKNVDFKQAASLTLVGPSAWQAMIQHIKLQPDQKIFIHGGAGGIG
                     IIAIQIAKHLGAHVATTASGGGIETVTKLGADEVIDYKKHNFIESLNDYDAVFDTVGG
                     DDFTKALSILKPGGIAVSTAGQADQAKANELGVTAIAQSTKVTTEALNELRLLVEEGI
                     VTPQVSKVFPLAAVSEAFRAWESGNVQGKVVIEVR"
     gene            complement(1109..1606)
                     /locus_tag="VF272_01835"
     CDS             complement(1109..1606)
                     /locus_tag="VF272_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7259650.1"
                     /translation="MQKFKPCIWFESGAEEAMNYYVSTFRNSKITHIERYSGDQGIPG
                     EEKLKGKVLTGVFELEGQEFMCLDGGPQFEMTGAISFLFEFETQEEIDEVWEKLTDGG
                     KPLQCGWVTDKFGLTWQITPVQLGRMLGDELATPEQKKAVMDAMLPMVKLDIAQLEAA
                     FEGAK"
     gene            complement(1634..2227)
                     /locus_tag="VF272_01840"
     CDS             complement(1634..2227)
                     /locus_tag="VF272_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259651.1"
                     /translation="MKFIGIAGGSGVGKSTLCYGLVDAEPEVFEVLNLDDYQYRKDVP
                     NLPVIHGQINWDHPDIIRWSDLMADLNKLRSGKSITIDVWSHRSNSNYFKHGQMISRT
                     LQPRPIVLIEGYLALYNSKLNEQYARKYYIDLNEATRAGRRKDKVIYEDDIVIPMHNK
                     YVEPTKKNADIIFNVGQMSIKEVQRKVKEDIIAAFLL"
     gene            complement(2306..2758)
                     /locus_tag="VF272_01845"
     CDS             complement(2306..2758)
                     /locus_tag="VF272_01845"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7259652.1"
                     /translation="MSALENPVLGTLAPNFSLQDQSGAVRNLYHALRQKSVFLFFYPY
                     DQSPSCTKQLCTINNELTLFQKDNLVAWGINNASAESHKLFADTRRLNLPLLSDEGYE
                     VSRKYDSLFSFGPIKVIRRTVVGISRDRKITFYAHGDMSLKDVLNQLI"
     gene            complement(2765..3190)
                     /locus_tag="VF272_01850"
     CDS             complement(2765..3190)
                     /locus_tag="VF272_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259653.1"
                     /translation="MKLSKLQILVAAGSLTVFGGLGFWIAESMASQNSNPIHHVSLTS
                     QGADPNVIAVVKGGYVQFDAKDGKQHKMSQGSGDDEVHHQAHTGVHEHQSDGKDSGMF
                     GLLEAYRVQFHQTGTFSFHDHLNPKLSITVVVYEAGRKK"
     gene            complement(3187..3594)
                     /locus_tag="VF272_01855"
     CDS             complement(3187..3594)
                     /locus_tag="VF272_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7259654.1"
                     /translation="MNRKVIILMVLGLVAVIVMVMASSSRPNHSTAYKMGNTSKQAGT
                     IQPADDKSVVIQNYTFNPNPLKVKKGTTVTWTNTDTAKHNVEVDDGQPAGGPNGPLFG
                     KNETYQFTFANAGIFKYHCSPHPYMHGSVEVTE"
BASE COUNT         1016 a          874 c          788 g         1056 t
ORIGIN      
        1 cttgaaaacc ggttaacgaa gctttatagt tactgccact atagcactcg caaacaaaat
       61 agactacttg ctataaaatg tatagtaaat tattattttc taacatgcta caaaaattaa
      121 agtaactcta gggggattat gaaagcagcg caaatcagcg agtacggcga tgcgtccgtt
      181 attactatta atgaaattga taagcctaac gtcggcaaga ggcagatcct ggttgaagtg
      241 catgcttcaa gtatcaaccc atttgacgca aaattacgcg ccggctatat gaaggaaagg
      301 attccgctgc aattccccgc tacgcttggc ggagatattg ctggcatcgt gtcggaacgt
      361 ggcgatgatg tgaccacggt agttgttggc gaccaggttt atggccaggc tgcgattgta
      421 gccggcggca gcggggcatt tgccgaatac gctgccacta aggctagcca aattgcaaaa
      481 gcaccaaaaa atgttgattt caagcaagcc gcatcgttga cacttgttgg cccaagtgct
      541 tggcaggcca tgatccagca cattaagctg cagccggatc agaagatttt tattcatgga
      601 ggtgccggcg gcattggcat tattgcgatt caaattgcca aacatcttgg tgctcatgtc
      661 gcaactactg ccagcggcgg cggcattgag actgtaacaa agctcggtgc tgatgaggtt
      721 attgattata aaaagcacaa ctttatcgaa tcactcaacg actatgacgc ggtttttgac
      781 actgttggcg gtgatgattt cacgaaagcg ctcagcattc tcaagccagg tggtatcgcg
      841 gtttcaaccg ccgggcaagc cgatcaagcc aaggccaacg agcttggcgt taccgctatt
      901 gcccaaagta ccaaggtaac aaccgaggcg ctcaacgaac ttcgattgct cgttgaagag
      961 ggtatcgtga cgccgcaggt tagtaaagtg tttccattag cagcagtcag cgaagcattt
     1021 agagcttggg aaagcggtaa tgttcagggg aaagttgtga tcgaagttag atagagttag
     1081 tcgtcaccaa tttccgctag tacgtgtgct acttagcgcc ttcaaatgcc gcttcaagtt
     1141 gggcgatatc aagcttaacc attggcagca ttgcatccat cacagccttc ttttgctcgg
     1201 gtgtagccaa ctcatcaccc agcattcttc ccaattgaac gggtgtaatt tgccaggtta
     1261 aaccaaactt atcggttacc cagccacact gcagcggctt acctccgtct gtcagctttt
     1321 cccacacctc atcaatttct tcctgggttt caaattcaaa taagaaagaa atggcgcccg
     1381 tcatttcaaa ttgaggacca ccgtctaggc acataaattc ctggccttca agctcaaata
     1441 cgccagtcaa tacttttccc ttgagcttct cctcgcccgg tatgccttgg tctccggaat
     1501 agcgctcaat atgagttatc tttgaatttc tgaaagtcga aacatagtaa ttcatcgcct
     1561 cttcggcacc gctctcgaac caaatacagg gcttaaattt ttgcatgcct acctcctaac
     1621 taataggagt tgattatagc aaaaaagctg cgatgatatc ttcctttact ttacgctgta
     1681 cctctttgat actcatctgc ccaacattaa aaataatatc agcatttttt ttggtcggtt
     1741 caacatactt attgtgcatc gggattacaa tgtcgtcttc ataaattact ttatcttttc
     1801 ttctgcctgc acgtgtcgcc tcgttcaaat caatatagta ttttcgagca tactgctcat
     1861 taagttttga attataaagt gccaggtagc cttctataag cacaattggt cttggttgaa
     1921 gcgtgcgaga tatcatctgg ccgtgcttaa aataattaga gttcgaccga tggctccaaa
     1981 cgtcaatcgt tatgctttta ccacttcgca gtttgttcag atccgccatc aaatcactcc
     2041 atcgaataat gtcaggatga tcccaattaa tctgcccatg gatcactggc agattaggca
     2101 catctttgcg gtactgataa tcatcaagat ttaatacttc aaatacttca ggctcggcat
     2161 caactaatcc atagcacaaa gtacttttcc caacgccaga gccgccagct ataccaataa
     2221 atttcatatc gtcagtatat caaatggctt ggcgcagcta aaagtctgtc ctgctccaag
     2281 acgatagcaa gtaagggcat acaagctata taagttgatt aagaacatct tttagcgaca
     2341 tatcgccatg agcataaaac gtaattttcc tatctcttga aatccccacc acagttctcc
     2401 tgattacttt aatgggacca aatgaaaaaa gactgtcgta ctttctagaa acttcataac
     2461 cctcatcaga tagtaaaggc agattaagcc gcctggtgtc ggcgaagagc ttatgggatt
     2521 cagcactggc attgtttatg ccccacgcaa ctagattgtc cttctgaaat aaggtcaact
     2581 cattattaat tgtacatagc tgcttagtgc aggaaggtga ttgatcatag gggtagaaaa
     2641 ataaaaagac actcttctgc cgcaacgcat gatagaggtt tcttactgcg cccgattgat
     2701 cttgcaaact aaagttagga gctaatgtcc caagtactgg gttctctaaa gcggacatta
     2761 aggcttactt ttttctgcca gcttcataca caactacagt tattgagagc ttaggattta
     2821 aatgatcatg gaaactaaat gtaccagttt ggtgaaactg aaccctgtaa gcttccaaga
     2881 gaccaaacat gcccgaatct ttaccatccg actgatgctc atggacaccc gtatgggctt
     2941 gatggtgaac ttcgtcatcg ccgctaccct ggctcatttt atgctgcttt ccatctttgg
     3001 catcaaattg aacatacccc cccttaacaa ccgctatcac gttaggatcg gccccttggc
     3061 tggtgaggct aacatggtga attgggttgc tgttttgtga tgccatagat tcagcaatcc
     3121 aaaacccaag gccaccaaag accgtcaagc taccggctgc aaccaatatt tgaagtttag
     3181 acaatttcat tctgttacct ccacagaccc atgcatgtaa ggatgagggc tgcaatggta
     3241 tttgaagatt ccggcattag caaatgtgaa ctgataagtc tcatttttgc cgaataacgg
     3301 gccgttcggc cctcctgcgg gctgaccatc gtcaacctct acattatgtt tagccgtatc
     3361 agtattagtc caagtcacgg tagttccttt tttgaccttg aggggattgg gattgaaggt
     3421 ataattttgg attacaacgc ttttatcatc ggctggctga attgttcctg cttgcttgga
     3481 cgtattaccc attttgtaag ctgtagagtg gttagggcgg ctgctagacg ccataaccat
     3541 gactatcact gcaacgaggc ccagtaccat taatataatt acttttctgt tcaatatgtc
     3601 tccttcacaa accagaagcc ggaattggct cctggttatc cttcagttta gaacttatct
     3661 gggctctgct taacaatcgc gccactaatg gcatcggcaa ttttaccgat ttgctcgttg
     3721 gcttcatgct cttt
//
LOCUS       DASVGB010000065         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_381283, whole genome shotgun sequence.
ACCESSION   DASVGB010000065 DASVGB010000000
VERSION     DASVGB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381283"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF272_01860"
     CDS             <1..314
                     /locus_tag="VF272_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HEX7259655.1"
                     /translation="PWSPPVGLNYLKACGTPSPNDEFDYWEPDVNDDNVVNQADVTIA
                     QSYVGQGDGRNYLYINDYVNANPQPETGPWRRYDMDMDGWVKDLDVNIVRSHLGKLCS
                     N"
     gene            492..1376
                     /locus_tag="VF272_01865"
     CDS             492..1376
                     /locus_tag="VF272_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018022432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX7259656.1"
                     /translation="MQKIILYYKFAPVPDPQAVMLWQKTLCESLGLKGRIIISKQGIN
                     GTLGGDVSALKKYAKATKLFQPFKGTVFKWSAGTADHFPKLSVKVRDEIVTFGAADEI
                     RVDEHGVVGGGQHLSPADVHKLVKERGGDVVFFDGRNVHEAAIGRFKNAVIPNVGHTR
                     EFREELKDAKYDAIKKKPVVTYCTGGIRCEVLSVLMKNRGFQEVYQIDGGIVKYGETY
                     KNKGLWEGSLRVFDDRMNMWFGETDDHIANCMVCGSKTSNQENCSGNGCRMLTVICED
                     CLQAKATIYCSPTCTSLS"
     gene            1527..3350
                     /gene="typA"
                     /locus_tag="VF272_01870"
     CDS             1527..3350
                     /gene="typA"
                     /locus_tag="VF272_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7259657.1"
                     /translation="MDQKPTHIRNIAIIAHVDHGKTTLVDGLLKQSKTFQDHQSEMGQ
                     ELIMDSGEQERERGITITAKITAVLHDDYRINIIDTPGHADFSGEVERTLGMADGCLL
                     IVDAQEGPMPQTKFVLTQALAHNLKPIVVINKIDKQASRVAEVEEELADLFLELAVHE
                     DQLHYPVYYAIGREGKAWKSIPYDPSASGDLTPIFEAIINDVPAPQVEPDKPLQMMVT
                     ALAFDSFKGKYAIGRINRGHVKAGDQVAVCKKNESIVKARVDAVFMSHGLSKFTVDGG
                     VAGDIVQLTGLGDVQIGETVADAANPEALPVLEVEAPTLQVYIGPNTSPFKSREGKFV
                     TGQQIGARLYKELEVNIGLKVEDTGLGYLVSGRGELHLSVLIEAMRREGFELEVGRPQ
                     VVTRENKGKTEEPVEEVIIEVPAEHAGTVQMEFGLRRATIKDQFNSPKGVTKLVYELP
                     TRALLGMRTILMTKTKGTAIMNSLVMGYQPIGPSLRQLRNGALIAFESGTTTPFALQN
                     AQERGTTFIGPAEQVYTGQVVGLNSRAEDMEVNVCKGKHLTNMRASSSDGIVKLTPHI
                     IFSLEECLDFIENDELLEVTPKSLRLRKRELEASKRKKAQK"
     gene            3409..3594
                     /locus_tag="VF272_01875"
     CDS             3409..3594
                     /locus_tag="VF272_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259658.1"
                     /translation="MRVLLAYLDPGSGSIIWQAIIGGAIGGVYVLRTTFGRIVAAGRA
                     AFYNLSRASDKTDKDSK"
     gene            3728..>4693
                     /locus_tag="VF272_01880"
     CDS             3728..>4693
                     /locus_tag="VF272_01880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7259659.1"
                     /translation="MSSGLYDNLIKQHLLIDHQEVARPSGLKLEAQHYKLLRPRQLNF
                     ISYPYEWSFSQYKDAALVTLRIQQEALKHGMVLKDASAYNIQFVDGRPMLIDTLSFES
                     LAHMQAWTAYKQFCQHFLAPLALMSKVDVRMGGMMRSYIDGIPLDLVAALLPRHQRLR
                     PGLLIHLWLHAKAQQRFAGGSKVSAKPRAVAGGNSRVLVAQADSLRRLVASLRLPAKH
                     TTEWGEYYTFTNYEEKSFQAKQKLVASYIKKVKPPVVWDLGGNDGRFTRVAVEAGAEK
                     AICFDIDPIAVEKNYHYSKKHPETAVLPLLLDLTNPSPALGWANRE"
BASE COUNT         1236 a         1043 c         1213 g         1201 t
ORIGIN      
        1 ctccgtggtc cccacccgtt gggttgaatt acctcaaagc ctgcggcacg ccctcaccca
       61 atgacgaatt tgactattgg gagccagacg tgaacgatga caatgttgta aaccaagcag
      121 acgttactat agctcagtca tatgtcggtc aaggtgatgg ccgtaactat ttatatatca
      181 atgattatgt aaacgccaac ccgcagccag agaccggccc gtggcggcgc tatgatatgg
      241 atatggatgg gtgggttaaa gacctcgatg tgaatattgt cagaagccac cttggcaagt
      301 tgtgcagcaa ctagacgcaa tctaacgatt acagataatc tagctttcat tttgaagcgt
      361 gacttagagc cgaaagatcc tttatgccaa cgggacctag aacatatccc tgatcatgta
      421 acagcagaag actgattaca tctcctactg atatactgta aagctatcaa tgcttaaaca
      481 gttgaacggc aatgcaaaaa atcattctct attataagtt cgcccctgtt cctgatccgc
      541 aggctgttat gctctggcag aaaacgctat gtgaaagcct tggcttaaag ggccgcatca
      601 ttattagtaa acaaggcatt aatggtactc tcggtgggga cgtgagcgcg cttaaaaaat
      661 acgctaaagc tacaaagttg ttccagcctt ttaagggcac ggtattcaaa tggagtgctg
      721 gcacagcaga tcattttccc aagctcagcg taaaggtgcg cgacgagatt gttacctttg
      781 gtgctgctga tgaaataagg gttgatgaac acggcgtggt tggcggcggg cagcatttaa
      841 gtccggctga tgttcataag cttgttaaag agcggggcgg ggatgtggtg ttcttcgacg
      901 gccggaatgt tcacgaagcg gctatcggcc gtttcaaaaa tgccgtgatc cctaatgttg
      961 gccatacccg cgaatttaga gaagagctta aggacgcaaa gtacgatgca attaagaaaa
     1021 agccggttgt gacgtactgt accgggggta ttcgctgcga agtactgtcg gttcttatga
     1081 aaaaccgtgg ctttcaagaa gtctatcaaa ttgacggcgg cattgtgaaa tacggagaaa
     1141 cgtacaagaa taaaggacta tgggaggggt ctttaagggt atttgatgac cgcatgaata
     1201 tgtggtttgg cgagaccgat gatcacattg ccaactgcat ggtttgcggg tcaaaaacca
     1261 gcaaccagga aaactgttct ggtaacggct gccgcatgct tacggtaata tgtgaagatt
     1321 gtcttcaagc caaggcaaca atctactgca gcccaacttg cacgtctctg tcttagcagc
     1381 cattgcttta cggcgggaaa accagcatta caaggaaatg cactagatgc aaagccttaa
     1441 tgcgctacct gtaaatcctc gtattttact tcaatctatg ctatagttaa taggtcttaa
     1501 acgacgcccc gcggcgttat ttttttatgg atcaaaaacc tacccacatt cgaaatatcg
     1561 ccattattgc tcacgttgac catggaaaga ctaccttggt agacggtttg ttaaagcaat
     1621 ctaaaacctt ccaggatcat cagtctgaga tgggccaaga actgattatg gacagcggtg
     1681 agcaggagcg tgaacgaggg attaccatta ccgccaaaat tacggctgtt ttgcatgatg
     1741 attatcgtat taacattatt gatacgcccg gtcatgccga tttcagcggc gaggttgaac
     1801 gcacgttggg tatggctgat ggttgcttgc tgattgttga cgcccaggag ggtccaatgc
     1861 cgcaaactaa gtttgtgcta actcaggcgc ttgcgcataa cttaaagccg attgttgtta
     1921 tcaataagat tgataagcag gctagccggg tggcggaggt tgaagaagaa ctggccgact
     1981 tgtttctcga attggcggtt catgaggatc agctgcatta ccctgtgtat tatgccattg
     2041 gacgcgaagg aaaagcctgg aaaagtattc cttatgatcc tagcgcatca ggcgatctta
     2101 cgcctatttt tgaggcaatc atcaacgatg ttcccgctcc tcaagtggaa ccggataaac
     2161 cgctccaaat gatggttacg gccctggcct ttgatagctt taaaggcaag tacgcgattg
     2221 gccggattaa tcgtggacac gttaaagctg gcgaccaggt cgcggtttgt aaaaaaaatg
     2281 aatcgatcgt taaggcgcgg gttgatgcag tatttatgag tcatgggctc agtaaattca
     2341 cggtcgacgg gggcgtggcc ggcgatattg tgcaactgac tggtcttggc gatgttcaga
     2401 taggggagac cgtggccgat gccgccaacc cggaggcgct tccggttctg gaggttgaag
     2461 ccccaacgtt gcaagtgtat attggcccca acaccagccc attcaaaagc cgggaaggga
     2521 agttcgttac cggccagcaa attggagccc gcctttataa ggaactcgag gtcaatattg
     2581 gtttaaaggt tgaagatacc ggcttgggct atttggtttc tgggcgcggc gagcttcatc
     2641 taagtgtttt aattgaagcc atgcgccgag aaggctttga gctagaagtt ggtcgtcccc
     2701 aagtcgttac tcgggagaat aaaggtaaga ccgaagagcc ggttgaagaa gttatcattg
     2761 aagtaccagc cgaacatgcc ggtaccgttc aaatggaatt tggattgcgg cgagctacca
     2821 taaaagatca gtttaatagc cccaagggtg tgactaagct ggtgtatgag ctgcccaccc
     2881 gggcattatt gggtatgcgt actattctta tgaccaagac caagggtact gctattatga
     2941 actccttggt tatgggttat cagccgattg gtccctcctt gcgtcaactg cgcaacggcg
     3001 cgcttattgc ctttgagtcc ggtactacga cgccctttgc attgcaaaat gctcaagagc
     3061 gcggcaccac gtttatcggt ccggctgaac aggtgtatac cgggcaggtt gttggcctaa
     3121 actcccgcgc cgaggatatg gaggttaatg tatgcaaggg aaagcattta accaatatgc
     3181 gtgccagcag cagcgacgga attgtaaaac taaccccgca tatcatcttt agccttgagg
     3241 aatgtttgga tttcattgaa aatgatgaac tcttagaagt aacaccaaaa agtttgcgtt
     3301 tacgcaagcg tgaactcgaa gccagtaagc gcaagaaggc ccagaagtag ttcttgtgtt
     3361 aaaatataag gataattcaa taaagattta agaaaaaaga ggtaagcttt gagagttctg
     3421 ttagcgtatt tggatcccgg ttccggaagt attatttggc aggcaattat tggcggtgca
     3481 attggcggag tctacgtttt acgtactacg tttggcagaa tcgttgcagc cggccgggcc
     3541 gccttttaca acttaagccg cgcatccgat aaaaccgaca aagattcaaa gtagtaatct
     3601 aagcagtatg acgaatccca accagctgca gtcctccttt cgggatcctg cagggcaggt
     3661 gtttttattc cggaacatga tttatcgcca ggtaaatttg gctggtaaaa cggattatga
     3721 tgcttttatg tcatcaggcc tgtacgataa cctcattaag cagcacctac ttattgatca
     3781 tcaagaagtt gccagaccat ccggcctcaa acttgaagcg cagcattata aactgcttcg
     3841 gccgcgccag ttaaacttca tctcataccc ctatgaatgg tcgttttccc agtataagga
     3901 tgcggcgcta gtcaccttgc gtattcagca ggaagcgtta aagcatggca tggtgcttaa
     3961 ggatgcttca gcctacaata tccagtttgt tgacggacgc ccaatgctca ttgacaccct
     4021 gtcgttcgag tcgttggccc atatgcaagc ttggaccgca tataagcagt tttgccagca
     4081 ctttctggcc cctctagccc tcatgtctaa ggtagatgtt cgtatgggcg gcatgatgcg
     4141 cagctatatt gatggtattc ccttggatct ggtggcagca ttgcttccgc gccatcaacg
     4201 attacgcccg ggattgttga tccatctttg gctgcacgcc aaggcgcagc aacgctttgc
     4261 cggcggctcc aaggtctccg ctaagccgcg ggcggttgcg ggcggtaatt ccagggtgtt
     4321 ggttgcacag gccgatagct tgcggcggtt ggtagcttcg ttacggcttc ccgccaaaca
     4381 cactacagaa tggggcgagt actacacctt tactaattac gaggaaaaat cctttcaagc
     4441 caaacagaag ctggtagcca gttatattaa aaaggttaaa cctccagtcg tctgggactt
     4501 gggtgggaat gacggacggt ttacccgcgt tgcggttgaa gccggagccg aaaaggcgat
     4561 ttgttttgat attgatccga ttgctgttga aaaaaattat cattacagca aaaaacatcc
     4621 ggaaacggcg gttttaccgc tcttacttga tttaaccaac ccaagtccgg ccttgggttg
     4681 ggctaaccgg gaa
//
LOCUS       DASVGB010000066         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_969333, whole genome shotgun sequence.
ACCESSION   DASVGB010000066 DASVGB010000000
VERSION     DASVGB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969333"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VF272_01885"
     CDS             complement(<1..574)
                     /locus_tag="VF272_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7259660.1"
                     /translation="MRAAIIGYGVEGALALKYWHNLGYQVCVHDANRQLELPDVIESH
                     LGPDYLKNLDQYDLIVRSPGVRPQLILQANPNLNATQITSVTTEFLAQCPAKVIGVTG
                     TKGKGTTATLITKMLEAAGYTVHLGGNIGHPPLDFLDQVEPEDWVVLELSSFQLMDIK
                     QSPQVAVMLMIATDHLNWHADLHEYLSAKQN"
     gene            complement(580..3156)
                     /gene="secA"
                     /locus_tag="VF272_01890"
     CDS             complement(580..3156)
                     /gene="secA"
                     /locus_tag="VF272_01890"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7259661.1"
                     /translation="MNLLKRIFGDPDAREVNKARLKVNQSNALEAKLKAFSDAKLKSQ
                     TAKLRGELEKGESLEAILPEAFATVREAARRTIGLRHYDVQLIGGSVLHDGKIAEMRT
                     GEGKTLVATAPSYLNALTGKGVHVVTVNDYLARRDPGWMGEVFHALGMSVGIIVPEAT
                     FGVSAYMFDPEYSDETADDERLKHLRPVSRQEAYNADITYGTNNEFGFDYLRDNMVSD
                     ASQMVQRPLHYAIVDEVDSILIDEARTPLIISAPAEESTEKYGQFAGLARMLKHEDDY
                     TLDEKQKAASLTDDGIRKLEKALGVDNIYEAGMIEDVHHVEQSLKAEALFKKDRDYVV
                     RDGEVIIVDEFTGRLMHGRRYSDGLHQAIEAKESVEIRKESLTLATITFQNYFRLYEK
                     LSGMTGTAATEAEEFGKIYHLEVTTIPTHQNMVRHDHADRIYRTELGKFNAVVVDVKE
                     RHQKGQPVLLGTVSISKNEILSELLNQAKVPHELLNAKNNEKEAAIVAAAGQKGAVTL
                     ATNIAGRGTDIVLGDGVDKLGGLHVVGTERHESRRIDNQLRGRSGRQGDPGSSRFYVS
                     LEDDLMRIFGGERVASLMQTLGLDDQTPLESPVVSRSLESAQKKVEGHNFDIRKQLVE
                     YDDVMNKHREVIYGRRRKVLKSRQLREDIEPMLSKEFKELVTTHTDVRTGVADTDAII
                     TQAGTLMPLSPEVEKVLQSADPRDLAAILTDYAQQLYDQREKEYTPDNMRMLERIVYL
                     QILDRLWIEHLEAMDRLREGIGLRAIGQRDPLVEYKSEGFRLFKRLLSLIEAEIAGTI
                     LRAQMSPTPASAQMETAVTKAAQKAQVSSDTSQAGAPGSDGGDGIAGNRAARRAKKRK
                     RR"
     gene            complement(3205..3591)
                     /gene="raiA"
                     /locus_tag="VF272_01895"
     CDS             complement(3205..3591)
                     /gene="raiA"
                     /locus_tag="VF272_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7259662.1"
                     /translation="MIQLQTTGRHYELDEKIMQYIDKKLGGLDRYLPRGVRGGLAGKV
                     ILECNEALSQDERCICEVQFEVKGEQFKAHESTINMYAAIDICEQKLKSQALTYKSKH
                     EPAKNRRQRLLAKMLGRSPLPEPLEE"
     gene            complement(3630..>4417)
                     /locus_tag="VF272_01900"
     CDS             complement(3630..>4417)
                     /locus_tag="VF272_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX7259663.1"
                     /translation="FALMMYGIQVLTDETKLAEIAKDAGLDVSTKPFTPVLPPLMIRT
                     EMFEAMDRLDPRDDRYKIEDEDLWLQGSAEHVLGSMHYNEIIPEAELPVRYVGYATSF
                     RREAGSYGKDMEGIFRLHQFDKLEMESLTAPEKSLDEHLFMVAIQEYLMQQLGLHYQV
                     IMKCTADIGKPNARGVDINVWLPGQAKYRETHTADYMTDYQARRLSTRLRREAGETEL
                     VHTNDATVFPQRPLIAIIENFQTEDGKVKVPEVLQSAYGGAWL"
BASE COUNT          999 a         1245 c         1070 g         1103 t
ORIGIN      
        1 tgttctgctt ggcactcaaa tactcatgca aatcagcatg ccagttcaga tggtcggtgg
       61 caatcattag cataaccgcc acctgaggac tctgcttgat atccatcagc tggaaactgg
      121 aaagctccag cactacccaa tcctcgggct caacttgatc caggaagtcc agtggcgggt
      181 gcccaatgtt gccgccaaga tgcaccgtat aaccggccgc ttcaagcatc ttggtaatta
      241 aggtggctgt ggtgcccttg ccttttgtgc cggtaacgcc aatcacctta gccggacact
      301 gcgccagaaa ctcagtggtt accgaggtaa tttgggtggc gttaagattc gggttggctt
      361 gtagaataag ctgggggcgg acacccggtg agcgtacaat aaggtcatac tggtcgagat
      421 tcttcagata atctggcccc aggtgggatt caatgacatc aggcagctca agctggcggt
      481 tagcatcgtg cacgcacacc tgataaccca gattatgcca gtacttcaac gccaaggcac
      541 cttcaacccc atagccaata atggcagccc tcacaacggt taccgccgtt tccgcttttt
      601 tgcgcggcga gccgcccggt ttccagcaat accatcacca ccatctgaac ccggtgcacc
      661 ggcctgtgag gtgtcgctgc tcacttgagc cttctgagca gccttggtta cggcagtctc
      721 catttgagcc gaagccggtg tcgggctcat ttgagcccgt aagatggtac cggcaatttc
      781 tgcttcaatc agtgaaagca gacgcttaaa caagcggaag ccctcacttt tatactccac
      841 caacggatca cgctggccga tggcccgcaa gccgatgcct tcgcgcagtc gatccatagc
      901 ttcaagatgc tcaatccaca gccggtcgag gatctgcaaa tagactatac gctccaacat
      961 gcgcatatta tcaggtgtgt actccttttc gcgctgatca tagagctgct gggcataatc
     1021 ggtgagaatg gcggccaaat cgcgcgggtc ggccgactgc agcacctttt caacctctgg
     1081 actgagcggc atcaacgtac cggcttgcgt aatgattgca tcggtgtcag ccaccccggt
     1141 gcggacatca gtgtgcgtgg ttaccagttc cttaaactcc ttggacagca tcggttcaat
     1201 atcttcgcga agctggcggc tctttaatac tttgcggcgc cggccataaa taacctcgcg
     1261 atgtttattc ataacatcat cgtattccac cagctgttta cgaatatcga agttgtgacc
     1321 ctcaactttc ttctgggcac tttctaagct gcgactgaca accggactct caagcggggt
     1381 ctggtcatct agtcctaagg tttgcatcag actagccact cgctcgccac cgaaaatacg
     1441 catcagatca tcttctaggg aaacgtagaa ccggctggac ccggggtcgc cctgtcggcc
     1501 ggaacgtccc cgcagttgat tatcaatacg ccgggactca tggcgctcag tgccaacaac
     1561 gtgcaagccg ccaagcttat cgacaccatc acccagtaca atatcggtac cgcggccggc
     1621 aatgttggtg gccagcgtca ccgccccctt ctggccagcc gccgccacaa tggcagcttc
     1681 cttttcattg tttttggcat ttaacagctc atgaggcacc tttgcttggt tcaacaactc
     1741 ggaaagaatc tcattcttgg agatcgacac tgtacccaaa agcaccggct gccctttctg
     1801 atggcgctct tttacatcga caacaaccgc attaaattta ccgagctcgg tgcgatagat
     1861 cctatcggca tgatcgtggc ggaccatgtt ttggtgggtt gggatggtgg taacttccaa
     1921 atggtagatc ttaccgaact cttcggcttc ggtcgcagcc gtaccggtca taccggagag
     1981 tttttcatac aggcgaaaat agttctgaaa ggttatggta gccagggtaa gtgactcttt
     2041 gcggatctca acactctcct tggcctcaat agcttggtgc agaccgtcgc tgtagcgtcg
     2101 gccgtgcatg aggcgtccgg taaattcatc gacaatgatg acctcgccgt cacgcaccac
     2161 ataatcacgg tccttcttga aaagagcttc ggctttcaag ctttgttcaa catgatgaac
     2221 atcttcaatc atgccggcct catagatgtt atcgacgcca agcgcctttt ccagcttacg
     2281 aataccgtcg tcggttaagc tggccgcttt ctgtttttca tccagggtgt agtcgtcctc
     2341 atgcttgagc atccgagcca aaccggcaaa ttgaccatat ttctcggtgc tttcttcagc
     2401 tggggccgag ataatcaacg gcgtccgagc ctcatcaatc aggattgaat caacctcgtc
     2461 gacgatggcg taatgcagcg ggcgctgcac catctggctg gcatcagaaa ccatgttgtc
     2521 gcgcagataa tcgaacccaa actcattgtt ggtgccatag gtgatatcgg cgttataagc
     2581 ctcctgccgt gaaaccggac gcaaatgctt cagccgctcg tcatcggcag tctcgtcaga
     2641 atactccgga tcgaacatat aagccgagac cccgaacgtg gcttccggta cgatgatccc
     2701 caccgacata cccaacgcat gaaacacctc acccatccaa ccggggtcgc gccgggctag
     2761 gtaatcgttt accgtgacca cgtgaacgcc cttgccggtt agcgcattta agtatgaggg
     2821 tgcggtcgcc accaaggttt tgccttcacc ggtacgcatt tccgcaattt tgccgtcatg
     2881 cagcacactg ccgccaatca attgaacatc ataatggcgc aagccaatgg tacgacgtgc
     2941 agcctcgcga accgttgcaa aagcttcagg aaggatagct tcgaggcttt cgcccttttc
     3001 tagctcgccg cgcagtttgg cggtttggct tttgagttta gcgtcgctga aggccttaag
     3061 cttagcttcc aaggcattcg actggttaac cttcagtctg gccttattaa cttcgcgagc
     3121 atcaggatcg ccaaaaatac gttttaacag attcataacc tgaatattgt agcacaggcg
     3181 aggtgagtac cccggctttg cagactactc ttctaacggt tccggtaacg gcgagcgtcc
     3241 cagcatttta gcaagcaagc gctggcgacg gttcttagcc ggttcatgtt tgcttttgta
     3301 ggtgagggct tgactcttca gcttctgctc gcagatgtcg atggcggcat acatattaat
     3361 ggtcgattcg tgcgccttaa actgctcccc tttcacctca aattgcacct cgcaaataca
     3421 gcgctcgtct tgcgacaagg cctcgttgca ttccagaata accttgccgg ccagcccgcc
     3481 gcggacaccg cggggtagat acctgtcgag accccctagt tttttatcaa tgtactgcat
     3541 tattttttca tcaagttcat aatgcctacc ggtcgtttgt agctggatca tatcaccctc
     3601 catttgcctg tcattattct agcgtacttc taaagccagg cgccaccata agcgctctgc
     3661 agcacctctg gcaccttgac tttcccatct tcagtctgaa agttttcaat aatggcaatt
     3721 aacgggcgct gcgggaaaac ggtcgcatca ttggtgtgca ccagctcggt ttcaccggcc
     3781 tcgcggcgca accgcgtact caagcggcgg gcttgatagt cggtcatata atcagcagtg
     3841 tgggtttcgc ggtatttagc ttgccctggc agccacacat taatatcaac gccccgggcg
     3901 ttcggttttc caatatcggc ggtacatttc atgatcactt gataatgtag acctagctgc
     3961 tgcattaaat actcctggat cgctaccata aataagtgtt cgtccaacga tttttcagga
     4021 gcggttaagc tttccatctc cagcttgtcg aactgatgca gacggaaaat gccttccatg
     4081 tccttaccat acgatccggc ttcgcggcgg aaactggtag cgtatccaac gtaacgcacc
     4141 ggcagctcgg cttcgggaat aatttcattg tagtgcatcg agcccagcac atgctcggca
     4201 ctgccttgaa gccaaagatc ttcgtcttca atcttataac ggtcgtctcg cgggtcgagg
     4261 cggtccattg cctcaaacat ttcggtacgg atcatcaacg gcggcaaaac cggagtaaat
     4321 ggcttagttg acacatccaa cccggcatcc ttggcaattt cggccagctt ggtttcgtcg
     4381 gttaatacct gaatgccata catcatcagc gcaaact
//
LOCUS       DASVGB010000067         4590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_625079, whole genome shotgun sequence.
ACCESSION   DASVGB010000067 DASVGB010000000
VERSION     DASVGB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625079"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VF272_01905"
     CDS             complement(<1..650)
                     /locus_tag="VF272_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7259664.1"
                     /translation="MNEEIISQVEELGLSNKEARVYIANLMLGPAGVQQIADASGIKR
                     VTTYVILESLVNLGLVSQTSKAKKTLFNAESPEHLRRLLQRREQSLQDQKQQLEELMP
                     ELKNMKSLPKDVPSVKFYDSAEGIRTAMKTVIDEARADGVKQLYGMSNLDQLHAFFPE
                     IAAESSNPERVAAGIASQYIYTSTRGRVYLPDDRENNRHSCYVPIDQYPLNGDMTVV"
     gene            806..2302
                     /locus_tag="VF272_01910"
     CDS             806..2302
                     /locus_tag="VF272_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX7259665.1"
                     /translation="MLAKTLAAAHAGMQGVLVEVECDISNGLPSLVVVGLGNQAVNEA
                     RDRIRGALRNSQLHIPPKRITLNLAPANLPKDGTAYDLSMALAILIATGQLEPTESCM
                     FMGELALDGSLRPIPGILAHAHLAANEGVQKLFVPHANAAEAALIEGVAVYGAASLVA
                     LYGHLAGLQPLETAQPLPPSKASGRSDLPDFSHVYGQPEAKRALEIAAAGGHNVLLSG
                     PPGTGKTMLSRALIGILPPPSMPEIIEISTLHSLAGKEIGAAKQRPFRNPHHSASSVA
                     LIGGGRVPRPGEISLSHRGVLFLDELPEFRRDVLEVLRQPLEDGCITIARASGVVAFP
                     AQFLLIAAQNPCPCGYAGDPHRRCNCSQNSITRYNQKISGPLLDRIDLLVQVRRVDPG
                     RLGKSNQEESSTVVAERVAAARAKQYSRTGKPELLNAYLPASAVRRDCQLEPEALALL
                     HQAMRNLSLSVRAYTRTIKVARTIADLEAAQTLTAGHIAEALRYRPKS"
     gene            complement(2292..2624)
                     /locus_tag="VF272_01915"
     CDS             complement(2292..2624)
                     /locus_tag="VF272_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259666.1"
                     /translation="MAKKKNKHKHSRFTRKQAVQPKVAASSALASSPSSSPAPSTASP
                     QTAALKASATQPKPGILNSSNRWYYVSGDLRRISVLASLCIGLELVLWYALSYTSFGD
                     SVYLLFTI"
     gene            2776..3258
                     /locus_tag="VF272_01920"
     CDS             2776..3258
                     /locus_tag="VF272_01920"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX7259667.1"
                     /translation="MNVTALSLVVIGIVAAVLIAGYCLWQVMYLRRRFTSALSNRDGD
                     SLEQTLATYLKRVETVDAHLGQLDDAYKRLADSGSLASQKISIVRFNPFGDTGGDQSF
                     VLAVLDAHNSGYVITSIHGREGTRVYVKPIDYGKSKYQLSEEEKKALAQATKRVPATT
                     "
     gene            3268..3663
                     /locus_tag="VF272_01925"
     CDS             3268..3663
                     /locus_tag="VF272_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7259668.1"
                     /translation="MAKKREVFTVGGIDTAIGSSVKLRGNLETDGDVAIDGQLVGNIK
                     SGGQLVVGINGQVKGNVEAVSVTSAGRIEGNIAAVDSVSLMESAQVRGDIASGRLEIA
                     MGAIFIGISKMKPAEATELLDRTKVDSAS"
     gene            3663..4529
                     /locus_tag="VF272_01930"
     CDS             3663..4529
                     /locus_tag="VF272_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7259669.1"
                     /translation="MYYYIINPAAGKGAINTIQTKLRNRLRELGIDGEFAKTTGPHDA
                     AKMTSIAIEKGHTTIVVVGGDGTVNEVVNGITNDTVAVGIIPMGTSNTLAHRMGITSW
                     QQACEVLAARRITSYGLIAAGRKYFLSTLTLGFETDLDKQVEMVPDANLRSRISQFAR
                     GWSHAKQFETLQCKIIIDNQLQLEAPLFSLSISNRKFEDPMADNKLQITLIDRPSAPR
                     LSHHLWKMLKKDQIEDGISQFSAKRVVIETTPPTGIMIDGKVSGRTPLAIRLTSKQIR
                     FITEKQTTSFKT"
BASE COUNT         1140 a         1226 c         1173 g         1051 t
ORIGIN      
        1 accaccgtca tatccccatt cagcggatat tggtcaatcg gcacatagca ggaatgacga
       61 ttattttccc gatcgtccgg caggtatacc cggccccgcg tactggtata aatgtactgg
      121 ctggcaatcc cggccgcaac ccgttccggg ttgcttgatt cggcagcaat ttccggaaaa
      181 aaggcatgaa gttgatccag gttgctcatt ccgtagagct gcttcacgcc gtcggcccta
      241 gcctcatcaa taacggtttt catagcggta cggatccctt cggcactgtc ataaaatttg
      301 acgctgggga catctttagg caaggacttc atatttttta gttctggcat aagttcttca
      361 agctgctgtt tttgatcttg caatgattgc tcacggcgct ggagcaaccg tcgcagatgc
      421 tccggagact cagcgttaaa aagggttttt ttggcttttg aggtttgact gacaagcccg
      481 agatttacca gcgactcaag gataacgtaa gtagtaaccc gcttaatgcc gctggcatcg
      541 gcaatttgct gcacgccggc cggacccaac attaagttgg caatatatac ccgggcctcc
      601 ttattgctga ggcctaattc ttcaacctgg ctgatgattt cttcattcat gtgcgcacat
      661 tgtaaagcac gcctttgagt ctgtaaataa cttttgacaa cacatgccct cgtcgactct
      721 ttctcttgga attattacca aaaaattaaa catttgcgat cgctgcgcca gccacgtagc
      781 gtgatggtat gaaaccaagc cgatcatgtt agcaaaaacc ctcgcggccg cccatgccgg
      841 catgcagggc gtcttagtgg aggttgagtg cgatattagc aacggcctgc cttcattggt
      901 tgtagtcggc ttaggcaatc aggccgtcaa tgaagcccgt gatcggattc gcggggcgct
      961 gcgcaatagc cagctacata ttccgcctaa gcgcatcacg ctgaacctgg caccagccaa
     1021 tctgccaaaa gacgggacgg cttatgacct gagcatggca ttggccatat tgattgctac
     1081 cggccagctc gaacctaccg aaagctgtat gtttatgggc gaactggctc tggacggcag
     1141 tttacggccg atcccgggaa tcctagccca tgcccatctg gcagcgaacg agggagttca
     1201 aaagctattc gtacctcatg ctaatgctgc cgaagccgca ttaatcgaag gtgtggcggt
     1261 ttatggtgcc gccagtctgg ttgcgctgta cggccattta gccggactgc aaccgctgga
     1321 aaccgcgcaa ccgcttccgc cttccaaagc gtctggacgc agcgaccttc ccgactttag
     1381 ccacgtctac ggccaaccgg aagcaaagcg ggccttagaa attgctgcgg ctggcggaca
     1441 caatgtgtta ctttcgggtc cacccggtac tggaaaaacc atgttgtcgc gagcacttat
     1501 cggtatttta ccgcctccga gcatgcccga aattattgaa atttctacac tgcacagttt
     1561 ggccggcaag gaaatcgggg cagcaaaaca gcgcccgttc cgcaatcccc accatagtgc
     1621 cagcagtgtt gctcttattg gcggagggag agttccgcgt ccaggagaaa tcagcctgag
     1681 ccaccgcggc gtactattct tagacgagct accggagttt cgacgcgacg tactggaggt
     1741 actccgccag cccctggaag acggctgcat taccattgct cgtgccagcg gggtggttgc
     1801 cttccctgcc caatttttac tgattgccgc acaaaacccc tgcccttgcg gctatgctgg
     1861 cgacccgcac cggagatgta actgcagtca aaactcaatt acccgctaca atcaaaaaat
     1921 ttccggtccg ctcttggacc gcatagactt actagttcag gtacggcgtg tcgatcccgg
     1981 gcgactgggg aagtccaacc aggaggaatc aagcacggtc gtggctgaac gggttgccgc
     2041 cgcgcgggct aaacaatata gccgcaccgg caaaccggag ctattaaacg cctatttgcc
     2101 agccagcgcg gtacggcgcg actgccagct tgaaccggag gctttggcct tattgcatca
     2161 agctatgcgc aatctcagct tatcggtacg agcctacacc cgcacgatta aagttgcccg
     2221 cacaattgcc gaccttgaag ccgctcaaac acttaccgcc ggccatattg ccgaagcgct
     2281 acggtatcgt cctaaatcgt aaacaaaaga taaactgaat cgccaaacga ggtatagctc
     2341 aatgcatacc agaggaccaa ttccaggcca atgcagagtg aagccaatac cgagatgcgc
     2401 cgcaggtcgc cgctgacata gtaccagcgg ttactgctat ttaatatgcc cggctttggc
     2461 tgagtggccg acgcttttag tgcagctgtt tggggtgatg cggtcgaagg agctggactg
     2521 ctcgaaggcg atgaagcaag cgcagatgat gcggcaactt tcggttgcac cgcctgcttc
     2581 cgggtaaaac gcgaatgttt atgcttgttc tttttctttg ccatagtgcc agtatactac
     2641 ggcagatctt cattgcagtg atttagtgtc ccaaggatac tacgtagtat ccttgggaca
     2701 ctattttaag tgaaacaata attcaccctc cgttccttgc agcctgtatg agcttgtacg
     2761 tatactagag aatacgtgaa tgttacagcc ttatctttag ttgttatcgg tatcgtagcc
     2821 gcagtcctaa ttgccggcta ctgcctatgg caagtcatgt acttgcggcg gcgttttact
     2881 tcggctttgt ccaaccgcga tggcgattct cttgagcaaa ctttggccac atatttaaag
     2941 cgggtcgaga cagttgatgc tcacctcggc cagcttgatg acgcctataa acggctagcc
     3001 gacagtggca gcctggcttc acaaaaaatt tccatcgtcc gcttcaaccc atttggcgac
     3061 accggcggcg accagagttt cgtgcttgct gtactcgacg cccataactc cggttacgtt
     3121 attaccagca tccatggccg cgagggcacg cgcgtatatg taaagccaat tgattatggc
     3181 aaaagcaagt atcagctttc agaagaagaa aaaaaggcgt tggcgcaagc taccaagcgc
     3241 gtaccggcaa ccacttaagg aggaatcatg gcgaaaaaac gcgaagtttt tacggtagga
     3301 ggaattgata cggccatcgg ctccagcgtt aagctgcgcg gcaatctgga aactgatggg
     3361 gacgtggcaa tcgacggcca actcgtaggc aatataaaaa gcggcggaca gttggtggtt
     3421 ggtatcaacg gacaggttaa aggcaatgtt gaggcagtga gcgtaacctc ggccggacgg
     3481 attgaaggca atattgcagc tgtcgattcg gttagcctga tggagagcgc tcaagtccgc
     3541 ggcgatattg ccagcgggcg tctggaaatt gcgatgggag ccatttttat aggtatcagt
     3601 aagatgaaac cggcagaagc caccgaacta ctcgatcgga caaaggtgga cagcgcctcg
     3661 taatgtatta ctacatcatc aacccagcag ctggcaaagg tgccattaat acgattcaaa
     3721 caaagttgcg caaccgtttg cgcgaactcg gtatcgatgg tgagttcgcc aaaactaccg
     3781 gaccacacga cgccgccaaa atgaccagca tagcgattga aaagggccac acgacaatcg
     3841 ttgtggtggg gggcgacgga accgtaaatg aagtcgtaaa cggtattacc aacgacacag
     3901 tagcggttgg gatcatcccc atggggacat ccaatacgct ggcccaccgc atgggcatta
     3961 ccagttggca gcaggcctgc gaggtactgg ccgcccgaag gataaccagc tacgggctca
     4021 ttgctgccgg ccgtaaatat ttcttatcaa ccctgacttt gggatttgag accgacctcg
     4081 ataaacaagt cgaaatggtc ccggacgcca acctgcgttc tcgcatcagc cagtttgcgc
     4141 gcggctggag ccatgccaaa cagtttgaaa ccttgcaatg caagatcatt atcgacaacc
     4201 agctacagct tgaggcgcca ctgttcagcc tgagtattag caaccgcaag tttgaggatc
     4261 cgatggctga caacaagctg cagattacct taattgaccg cccgtcggca ccgcggctca
     4321 gccaccatct ctggaaaatg cttaaaaaag atcagattga agatggcatt agccagttta
     4381 gcgctaaacg cgtagtcatt gaaaccactc ccccaaccgg gataatgatc gacggcaagg
     4441 tcagcggccg aactccccta gccattcgct taaccagcaa acaaatccgc tttattactg
     4501 aaaaacagac cactagtttc aaaacctgag ccgcaactgc ccttcattta caggttgacc
     4561 aagctgttta catctggtag cattattctc
//
LOCUS       DASVGB010000068         9945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1168232, whole genome shotgun sequence.
ACCESSION   DASVGB010000068 DASVGB010000000
VERSION     DASVGB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168232"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..233)
                     /locus_tag="VF272_01935"
     CDS             complement(33..233)
                     /locus_tag="VF272_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259670.1"
                     /translation="MMYSTNARRITVAEATARKALLTGRPLEISGWHIRVTGITVDPV
                     GKRAVGYIGDRRQMTCAVPIAG"
     gene            353..1033
                     /locus_tag="VF272_01940"
     CDS             353..1033
                     /locus_tag="VF272_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259671.1"
                     /translation="MERRMDQRGFGALEMVLAVLLLVAIGAAGYFAYQARQAQRDPVA
                     NVGTPKNAQSVESSNAGASWKTYKSNYEMTSFRYPANWTVKAETIPNLPNVPEAKMVT
                     LRSPQGFELHYFDVLTGLGGGCPETDPNVTFTTVQGLEHTNPKLYFIENESILALTDE
                     NSWRDKKPHTGDSGDCLFYPLFLSKIHKGGQMSFATAIQIRPNAEPLKASKVDLANAR
                     LILESFTY"
     gene            1065..1952
                     /locus_tag="VF272_01945"
     CDS             1065..1952
                     /locus_tag="VF272_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7259672.1"
                     /translation="MKAFSDIFIIYNPVSTGSSEPTAMKLERQLGKALPDVSIKSLPT
                     KYAGHAEELVYELATSHKKPLIISSSGDGGYHEVINGAMKAKKAKKVAITAVLPAGNA
                     NDHSRTMQERPLAEAIIAGKITAIDLLEVTIHTKGKKLVRYAHSYAGLGLTPVIATEL
                     NRHTLNAFRETWLVLTTFFKHRPFTIRHSNKLIILDSLIFTNINEMAKILTLAQDNKP
                     DDGKFEVISFPHAHKFLLIKKLFRAATVGLQTTRRENTYSFEVIKKMPLQLDGEVTTL
                     AAGARVKISAAPMALKTII"
     gene            2678..2956
                     /locus_tag="VF272_01950"
     CDS             2678..2956
                     /locus_tag="VF272_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX7259673.1"
                     /translation="MGLLEGLLAFRLIFKLLGANPASGFVTFIYSVTEPFVAPFYGIF
                     GQVTTTGAETVAVFEPATLIAMLIYALVAWLVVRLIGAASGRPVDREI"
     gene            complement(3170..3631)
                     /locus_tag="VF272_01955"
     CDS             complement(3170..3631)
                     /locus_tag="VF272_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259674.1"
                     /translation="MGGREAVRISLPGRSFFRVEANIGFFSMGFSGKWSCQEWLEYLS
                     ERYEISTAERVVVFVDGGHLDQAGAMLCIEEVLADARALEFVRFEFPSATYIYNVESD
                     EPQGGTELGGMYRRFDEEVTDLLIRGGSPAITAAMDELMPHLERLTMYLET"
     gene            complement(3789..3864)
                     /locus_tag="VF272_01960"
     tRNA            complement(3789..3864)
                     /locus_tag="VF272_01960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3829..3831),aa:Ile,seq:cat)
     gene            3962..4357
                     /locus_tag="VF272_01965"
     CDS             3962..4357
                     /locus_tag="VF272_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7259675.1"
                     /translation="MNPRIKKFLRRIHFGMVLGILVVGYISFYLVETVSYNYTLKRQI
                     DTLQDQLTDLQTQKQTLEYKIQYYQTDDYKEKQARAKLGLQASGEEVIILPHTDAAPK
                     PEENAKQSVSKKSNFQQWIDFLLGRQNSL"
     gene            4354..5937
                     /locus_tag="VF272_01970"
     CDS             4354..5937
                     /locus_tag="VF272_01970"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX7259676.1"
                     /translation="MKHLFKLILSLGLLFSSAASAHAAERIINFDSNIFIDKQNVADI
                     TETIQYDFGSDDRHGIYRYVPVDYTDDKNNTYHLALTYLETKDENGRKVQSENSKSNG
                     NFTMRLGDPDTTITGIHTYKIHYQLRPIVRQEGERGFLALDITGDGWEVPIERASASF
                     RFEDSVSLADGKCFSGPPGSTAGCLVQESFPATYVAQNLNPHEGMTVQGLLPAGYVAN
                     YLVAGKRAPLTAEDIQTFFFIGLAVLSGLFVGWIFLMRYLRERRRKKAQTIVAQYEPP
                     AALSPGEIGLLQDDVSGIREITAMLIDLSVRGFMKIEQTSPKKWYKKAKYTLRKVKEP
                     TGLVSYEQKLWNQLFSGRESIELERINKATMSAAITQIHADLKSRLKKKGFYGSLSSE
                     PGMMEKLIDTGNITDAGAVEWAKVEGLRLYLKVAEKDRLNFTDAPQRTPERFNKLLPY
                     AVALGVEKEWAKLFAGIDMQRSSGWYVGSYPHFSSSALASDLGGSFSSSFSSNATMSS
                     SSSGGSSGGGSGGGGGGSW"
     gene            6001..6315
                     /locus_tag="VF272_01975"
     CDS             6001..6315
                     /locus_tag="VF272_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259677.1"
                     /translation="MAVELVNKQYFDEKMLEQQAFMAKLFDVWMLEVAKKFASIEKRL
                     DKLEQRLDGVEQRLDKVENQLSEIQLDLNEVRSRVFDTAQRQEWLALVRRVEVLEKAL
                     RK"
     gene            complement(6329..6907)
                     /locus_tag="VF272_01980"
     CDS             complement(6329..6907)
                     /locus_tag="VF272_01980"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7259678.1"
                     /translation="MKNAIILHGSPTREEFYDPNIPNESNCHWLPWLQAQLVKADVPA
                     ATPEVPRPFEREWSIWTKEFERFDIGPETILVGHSTGAGFIIKYLSIHPDLRVNRVVL
                     VAPWLDPQREHTKDFFEDFEIDLDLVGRTNGIVIFNSDNDEDSVHETVAMLREKIRTI
                     QYREFHDHGHFCYRDMKTIKFPELLEEVLSEN"
     gene            6995..7351
                     /locus_tag="VF272_01985"
     CDS             6995..7351
                     /locus_tag="VF272_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259679.1"
                     /translation="MALTIEEKQYFDQRFERIELARKGDRIYFTGLFNAALREISKKF
                     DTVEKRLEAIEKRVTAVEQHLASVEQRMEQLERAVKQIKLDLHEVRARVFDTAQRTEW
                     LSLVSRVEALEKNCRR"
     gene            complement(7358..7432)
                     /locus_tag="VF272_01990"
     tRNA            complement(7358..7432)
                     /locus_tag="VF272_01990"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7398..7400),aa:Met,seq:cat)
     gene            complement(7459..8046)
                     /gene="pdxT"
                     /locus_tag="VF272_01995"
     CDS             complement(7459..8046)
                     /gene="pdxT"
                     /locus_tag="VF272_01995"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX7259680.1"
                     /translation="MKRTVGVLALQGDFVEHAASIERCGAQGILVRTAIELKQIDALI
                     IPGGESTTMGIIAESIGMVEPLQLFIASGLPVWGTCAGLIMLADKVEGGKRNGQTTLG
                     GLDIIAARNFFGRQIQSFSASLSVPKVATEPIDVMFIRAPGITRVGYKVEVLATVSHN
                     DSHVAVAVRQNNILATAFHTELGDDPKFHRYFLSF"
     gene            complement(8054..8938)
                     /gene="pdxS"
                     /locus_tag="VF272_02000"
     CDS             complement(8054..8938)
                     /gene="pdxS"
                     /locus_tag="VF272_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX7259681.1"
                     /translation="MTQKDTFAVKSGLAQMLKGGVIMDVVTAEQARIAEDAGAAAVMA
                     LERVPADIRADGGVARMSDPKMIKAIMKAVTIPVMAKVRIGHFVEAQILQELGVDYID
                     ESEVLTPADNEHHIDKHPFTVPFVCGCRNLGEALRRIQEGAAMIRTKGEAGTGNIVEA
                     VRHARTVTDEISSLQNAPPADVKAFANNERIAEDLLRQTLKLGRLPVVNFAAGGIATP
                     ADAALMMQLGVDGLFVGSGIFKSGDPAIRAKAIVLAATHYDDPKMLANVSENLGEPMV
                     GINLETLSDEERLAARGW"
     gene            complement(8963..9039)
                     /locus_tag="VF272_02005"
     tRNA            complement(8963..9039)
                     /locus_tag="VF272_02005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9003..9005),aa:Met,seq:cat)
     gene            9161..9481
                     /locus_tag="VF272_02010"
     CDS             9161..9481
                     /locus_tag="VF272_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259682.1"
                     /translation="MRYLGLACELGVYGELEKIDALTWGLADFSGYPLAIKSHSPELV
                     VLGVPTRNGGGTAPDMHLYLCGRDLTDLRAVYNEAKRVGSPGATITWYRFSDKLAPAL
                     QTTG"
     gene            9494..9766
                     /locus_tag="VF272_02015"
     CDS             9494..9766
                     /locus_tag="VF272_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259683.1"
                     /translation="MAGSTTLFDLDPLNPVHRALIAYGLALAARQLPLMNATSTAALQ
                     DAVRLTGISQDVLMPALAEFRQGFQAEYETNRRKHKANLMDSDVRW"
BASE COUNT         2535 a         2337 c         2617 g         2456 t
ORIGIN      
        1 gatccctgcg aaagcggggg agggcgaaag ccctagccgg cgatgggaac agcgcaggtc
       61 atctgacggc gatcgccgat gtagccaacg gcgcgcttgc cgacagggtc gacggtgatg
      121 ccggtaacgc ggatgtgcca accgctgatc tcgagcggcc ggccggtgag aagtgccttc
      181 cgtgcagtcg cctcggcgac ggtgatgcga cgggcgttgg tgctgtacat catgagagat
      241 gcaccccttt cgggtgttgc ttgacggagc tcgagctaca gggtatacct cgatatttgg
      301 ataataccac ataaaatgct accgtaaacc taacttaaag gtgagcatgt atatggaaag
      361 aagaatggac cagcgtggat ttggtgccct ggaaatggtt ttggcggtac tactccttgt
      421 tgctattggc gccgccggtt atttcgccta tcaggctcgt caagctcaga gggatccagt
      481 ggccaatgtc ggtactccaa agaatgcgca gtctgtcgaa tctagtaacg ctggcgccag
      541 ctggaaaacc tataagtcta actatgagat gaccagtttt cgctatccag caaattggac
      601 ggtaaaagca gaaacaattc ctaatcttcc caatgttcct gaagccaaga tggtaacgct
      661 aaggtcgccc cagggttttg agcttcacta ttttgatgtt ctcactggat tagggggtgg
      721 gtgcccggaa actgacccta atgtcacttt taccaccgtt caagggcttg agcatacgaa
      781 tccaaaactt tattttattg agaacgaatc aatcctcgcc ctgaccgatg aaaatagttg
      841 gcgagataag aaacctcata ccggcgactc tggcgactgc ttgttttacc cgttattctt
      901 aagcaaaatt cacaaaggcg gacaaatgag ttttgctact gctattcaaa taaggccgaa
      961 tgccgagcct ttaaaagcca gcaaggtaga tttggcgaac gcccgattga tcctagaaag
     1021 cttcacgtac taaaaaatct tttagtgtct gtttatatta cgggatgaaa gctttctcgg
     1081 atatcttcat catttataac ccggtaagca cggggagcag tgagccgact gctatgaagc
     1141 tggagcggca actaggcaaa gctttgcctg acgtatcgat aaaaagtctt cccactaaat
     1201 acgccggaca cgccgaagaa ttggtttatg aattggctac gtctcataaa aagcccctca
     1261 ttatctcttc cagcggcgat gggggctacc acgaagttat taatggcgcg atgaaggcaa
     1321 aaaaagcgaa gaaagtagcg attacggcgg tactgccggc tggtaatgct aacgatcata
     1381 gccggacgat gcaggagcgg ccattagcgg aggcgattat agccggaaag atcactgcta
     1441 tagatttact tgaggtcaca atacatacga aagggaagaa gctggtgcgt tatgcccact
     1501 cctatgctgg gttaggatta acgccggtaa ttgctacgga gctcaaccgc cataccctta
     1561 atgcattccg agaaacctgg ttggtgctga caaccttttt taagcatcgg ccatttacga
     1621 ttcgccatag taacaagctc attattttag acagtctaat ttttacgaat atcaatgaaa
     1681 tggcaaaaat tttaacctta gcccaagaca ataaaccgga tgatggcaaa tttgaggtaa
     1741 tttcatttcc gcacgcacac aagtttttac ttataaagaa attgtttaga gcggctacgg
     1801 ttgggcttca aactaccagg cgcgagaata cctattcatt tgaagtaatc aagaaaatgc
     1861 cgctgcagct ggatggagag gtgaccactt tggcggcagg ggcccgggtc aaaattagtg
     1921 ctgctccaat ggctcttaaa accatcattt agcccttata taggaggaag cccgtcgcaa
     1981 gcgacaggct tctcgaagaa agtgtcgccc gtaacgggct agttggcgag gcactcctcc
     2041 gtcggcaggt gatgccaaac gaagctagag ctgctcatcg atgctggggt gtgttgtaaa
     2101 ccgcttgcgg caaaggcgag gaacgccagt cgtaagtttt ccaggtatcg acacaaatgc
     2161 caggtgcgca tgttctgaat caaagtccgc ccatgaagat gtccagtaga catgggcgtt
     2221 cagcggttca gcactcagcg atgcgggtgc ccctgaaggg gcttgcacgg ctgtcatgca
     2281 gcaactcagc gacaagctcg tctttcctcg cccatgatgt atggacgaga tttgacgcgg
     2341 cgtcattgaa acggcattgc gacggatctg aaaagatcca aggagcggtt gagcaatgct
     2401 caggctttcc tcgtgtcgaa aaacggggga gtccaactca aatactttac aagaaatatg
     2461 acataaaatc aagcatttca ttttgctttc tccataactt ataaaagcac ttaaatctgt
     2521 aagttaggat attgcacaaa caaaaatcga tatgcttcac ttatacttgt atacattaag
     2581 ggaggaaaga atcatggccg aaagagttgt tgttgcagat gataccccgg cgcgtcgttc
     2641 aggtgcttgg agcgttgcga gcattattta cgtattgatg ggtttacttg aggggctctt
     2701 agctttcagg ctaattttta aattgcttgg cgctaatccg gccagcgggt ttgtaacctt
     2761 tatatatagc gttactgagc cctttgtggc accgttttat ggtattttcg gtcaagtcac
     2821 tacaacggga gccgagacag tagcagtatt cgaaccggca acattaattg ccatgctgat
     2881 atacgccttg gtagcatggt tggttgtgcg gctaatcggt gctgcttcag gccgtccggt
     2941 cgaccgggaa atttaaaaga agttgatttt ggtggacttg gttggatctc aatggaacca
     3001 aatcaagaag gagctgatag gctgtatcgg gcgctaagag atagcctggt ccttgccctc
     3061 tacggaaatt ggtttgaact attaatacga aaagagccct ggtattccaa ggcccttggg
     3121 gggcgagcgg ctccatacag gcccgctcca cccgcttctt gcggcacgtt taagtttcga
     3181 ggtacatggt gagcctttcg agatgaggca tgagctcgtc catggcggcg gtgatcgccg
     3241 gactccctcc tctgatgaga agatcggtca cctcttcgtc gaagcggcgg tacattccgc
     3301 caagctcagt tccgccttga ggctcgtcgg attcaacgtt gtagatgtac gttgcgctgg
     3361 gaaactcgaa gcgcacgaat tccagcgccc ttgcgtcggc tagcacttct tcgatgcaaa
     3421 gcatcgcacc tgcttgatcc agatgtccgc cgtcaacgaa caccaccacg cgttccgcgg
     3481 tcgaaatttc gtatctctcg gagaggtact cgagccactc ctggcacgac cactttccgg
     3541 agaagcccat cgagaagaag ccgatgttag cttcaacacg aaagaagctt ctgccgggaa
     3601 ggctgatccg cactgcttct cggccgccca ccctgtatga atgtatcttg agggacactg
     3661 ctttaccacc cttacttgag gaaagaagtg ggcaaataat actttaataa caaagaaatt
     3721 acaagtagtg cttgatccag tcggcgcatt taatataaaa acgaataata catttgagaa
     3781 aaatcaattg gtgggcccgg ctggacttga accagcgacc aatcggttat gagccgactg
     3841 ctctaaccac tgagctacgg gcccgcaagg atatagaacg gtacgcaccg attatgctgg
     3901 aaaacgcccg ccttgaccag cgcgctgtaa ttgttacggt tggcatacta caatgtaaga
     3961 tatgaacccc cgtatcaaaa agtttctgcg gcgcatccat tttggtatgg tgctgggtat
     4021 tcttgtggtt ggctacattt ccttttacct ggtggagacc gtgagctaca actatacatt
     4081 gaaacggcag attgatacgt tgcaggacca gctcaccgat cttcaaaccc aaaaacagac
     4141 gttggaatat aaaatccagt actatcaaac cgacgattat aaggaaaagc aggcacgggc
     4201 taaacttggc ctgcaggctt cgggcgagga agtgattatc ttgccccata ctgacgctgc
     4261 accaaagccc gaagaaaacg cgaaacaatc ggtaagcaaa aaatccaact tccagcagtg
     4321 gattgatttc ttgttgggca ggcagaactc actgtgaagc acttgttcaa gctgattctt
     4381 tcgcttgggc ttcttttttc tagtgccgca tcagctcacg cggccgagag gatcattaat
     4441 tttgattcca acatatttat tgacaagcaa aatgtcgccg atattaccga aactattcag
     4501 tatgattttg gttcagacga tcgtcatggt atttaccggt atgtgcccgt ggactatacc
     4561 gacgacaaga ataataccta ccatttggct cttacttatc ttgagaccaa ggatgaaaat
     4621 ggccggaagg ttcaatctga aaacagtaag agcaatggca acttcacgat gcgtttgggt
     4681 gatccggata cgactattac cggtattcat acgtacaaaa tccattatca actgcgtccg
     4741 atagttcggc aggagggaga gcgtggattc ttggcgctgg atatcactgg cgacggctgg
     4801 gaggtaccga ttgaacgagc gagcgcgagc ttcagatttg aagatagtgt ttcgctagcg
     4861 gacggtaaat gtttttccgg tccgccagga tcgacggccg gatgtttagt ccaggaaagc
     4921 tttccggcca cctatgtagc ccaaaatctt aatccccatg aaggtatgac cgttcaaggg
     4981 cttttgcccg ccgggtatgt tgccaattac ttagtcgccg gcaagcgagc accactgacc
     5041 gctgaagaca ttcaaacttt tttcttcatt ggcttagctg tgttaagcgg cctttttgtt
     5101 gggtggatct ttttgatgcg ctatctgcgc gaacggcggc gcaagaaagc ccaaaccatt
     5161 gttgcccagt atgaacctcc ggcggcttta tcgccgggtg aaatcggttt gttgcaagat
     5221 gacgtttcgg ggatacgaga aattaccgcg atgcttattg atttgtcggt acggggattt
     5281 atgaaaattg aacagacgtc gccaaaaaaa tggtacaaaa aggccaaata tacgcttcgt
     5341 aaagtaaaag aaccaactgg gttggtaagc tacgagcaaa aattatggaa ccagttattc
     5401 agcggccggg aaagcattga acttgagcgt attaacaaag ctacgatgtc ggctgccatt
     5461 acccaaattc atgcggattt aaaatcaagg ctgaagaaaa aagggtttta tggttcgctt
     5521 tccagcgagc cgggcatgat ggaaaaactg attgataccg gcaacattac cgatgctgga
     5581 gcggtagagt gggcaaaggt cgaagggtta agattgtatt taaaagttgc ggaaaaagac
     5641 cggttgaact ttaccgatgc tccacagcga acccctgagc gcttcaataa gcttctgccg
     5701 tacgctgttg ctctgggcgt tgaaaaagag tgggccaaac tgtttgcagg catagacatg
     5761 cagcggtcat cgggttggta tgtcggcagc tatccgcact tttcgtcctc ggcgctcgcc
     5821 agcgacttag gcggcagctt ttcatcgtct tttagcagca atgcaactat gtcgtcctct
     5881 tcatccggcg ggagctcagg cgggggatcc ggtggtgggg gcggaggcag ttggtagcat
     5941 cgtaattttt agttaatctt cgtttgctaa actggtaaaa ttgaatgtga gaggggtttc
     6001 gtggctgtag aactggtaaa taagcagtat tttgatgaga agatgctcga gcagcaggct
     6061 tttatggcca agttgtttga tgtttggatg ctcgaggtag ccaagaagtt tgcttctatt
     6121 gagaagcggc tcgataaact cgagcagcgt cttgatggag tagaacaacg gctggataag
     6181 gtggagaatc agttgagcga gatccagctt gacctcaatg aggtgcggag ccgtgttttt
     6241 gatacagccc agcgccaaga atggcttgca ctggtccgga gagtggaagt tttggaaaag
     6301 gcgctccgaa agtaaagcgg ttggtagttt agttttcgga taggacctct tcaagcagct
     6361 cgggaaattt gatcgttttc atatcgcggt agcaaaaatg accgtgatca tggaattccc
     6421 ggtactggat ggttctaatt ttctcgcgaa gcatcgcaac tgtctcgtgg actgaatctt
     6481 catcattatc cgaattgaag atgacgatac cattggtacg gcctacaagg tcgagatcaa
     6541 tttcaaaatc ttcaaaaaaa tctttggtat gctcacgctg cgggtcgagc catggggcaa
     6601 ctaaaacaac tcggttgacc cttaggtcgg gatgaatgct taaatattta atgataaatc
     6661 cggcgccggt gctatggcca acaaggatag tttcaggccc aatgtcgaat cgctcaaact
     6721 ctttggtcca gattgaccac tcgcgttcaa aaggacgcgg cacttccggg gttgctgctg
     6781 gcacatcagc ttttaccaac tgggcctgca gccacggcag ccagtggcag ttgctctcat
     6841 tggggatgtt tggatcataa aactcttcgc gagttgggct tccatgcaaa ataatggcgt
     6901 tcttcatggg gacattatag caaataacaa agttcatttt tatttaacca cgttttgtta
     6961 ggctgctatt attgtaataa tacgaggatc tgtgatggcc ctaaccatag aagaaaagca
     7021 gtatttcgat cagcgttttg aacgcattga gttagcgagg aaaggggatc gtatctactt
     7081 tactggcttg tttaacgctg cactgcggga aatttccaag aaatttgata ccgttgaaaa
     7141 gcggctcgaa gctattgaaa aacgggtgac tgcagttgaa cagcatttag cgagcgtaga
     7201 gcagcggatg gaacagcttg agcgtgctgt caaacaaatt aagctcgatc ttcatgaggt
     7261 gcgtgctcga gtatttgata ctgcccaacg gacagaatgg ctgtctctgg taagtcgagt
     7321 tgaggcatta gaaaaaaatt gtcgccgata aaatcaatgg tggctgcggg tagatttgaa
     7381 ctaccgacca aaggcttatg agtcctctgc tctaccactg agctacgcag ccataccacg
     7441 caattgtact cggttcgttt agaaactcaa aaagtagcgg tgaaatttag gatcatcacc
     7501 caactcggta tgaaatgccg tggcaagaat gttgttttgg cggacggcaa ctgcgacatg
     7561 gctatcgtta tgcgaaaccg tagctaagac ttcgaccttg tagccgactc tagtgatgcc
     7621 aggtgcgcgg atgaacatca catcaatcgg ttcagtggcg acttttggta cggacagtga
     7681 ggcgctaaaa ctttgaatct gccgtccaaa aaagttacgc gcagcgataa tgtccaaacc
     7741 gccaagggtt gtttggccgt tccgctttcc cccttcaacc ttgtcggcaa gcatgataag
     7801 accggcgcag gtaccccaaa ccggcaaccc tgatgcgata aacagctgca aaggttcgac
     7861 catgccaatg ctttcagcaa tgatgcccat ggtggtactt tcgccgccgg ggatgatgag
     7921 agcgtcaatt tgtttgagtt caatagccgt tctaaccaat ataccctgag cgccgcaacg
     7981 ttcaatggaa gctgcatgtt ctacaaaatc tccctggagg gctagcacac caacggtcct
     8041 cttcatggtg gtattaccag ccgcgggcgg ctaagcgttc ttcgtcggac aaggtttcaa
     8101 ggttgatgcc aaccatgggt tcgcctaagt tttcactgac gttagccagc attttggggt
     8161 catcgtaatg ggtagcggcc agcacaattg ccttggcgcg tatggccgga tcaccacttt
     8221 taaaaatacc ggaaccaaca aagagaccat caaccccaag ctgcatcata agcgcagcat
     8281 cggcaggagt ggcaatgccc ccggcggcaa aattgactac cggtaggcgg ccgagcttaa
     8341 gggtttggcg aagcaggtct tctgctatcc tttcattgtt tgcaaaggct ttcacatcgg
     8401 cgggaggggc attttgaaga gagctaattt catctgtaac ggtgcgggca tggcggacag
     8461 cttcaacaat attaccagta ccggcttcac ccttggtgcg gatcatggca gcaccttcct
     8521 gaatgcgccg cagagcttca cctaaattgc ggcagccaca aacaaatggc accgtgaaag
     8581 gatgcttgtc tatgtgatgc tcattgtcgg ccggagtcag gacttcgctt tcatcaatat
     8641 aatcgacgcc cagttcttga agaatctgag cttccacaaa atggccgatg cggacttttg
     8701 ccatgacggg tattgtcacg gctttcataa tggctttaat cattttcgga tcgctcatac
     8761 gggcgacgcc gccatcagcg cgaatatccg ctggcacccg ctcgagcgcc ataacggcag
     8821 cggcaccggc atcctcggca atgcgggctt gctcggcggt aacgacatcc ataataacac
     8881 cgccctttag catctgagct aggccgcttt taacggcaaa tgtgtctttt tgtgtcataa
     8941 aggaattctt tgcataattg cttggtagcg ggggcaggat tcgaacctgc gaccttcggg
     9001 ttatgagccc gacgagctac cggactgctc caccccgcga cgacgttttt caaggatacc
     9061 aggtctaagg tgttgggtca acgattctag ggtagtattt taaaataatt agtataaaat
     9121 aatcatacct ctgcaattaa catggaaggt aggatctctc atgagatacc tcggactggc
     9181 atgtgagctt ggcgtttacg gggagctcga gaagatcgat gctctcacct gggggctcgc
     9241 tgacttctcc ggttacccgc tggcgatcaa gagccacagc ccggagctgg tcgtactggg
     9301 cgttccaact cgcaacggcg ggggcactgc tccggacatg catctctacc tctgcgggag
     9361 ggatctgacc gatctgcgcg ctgtttacaa cgaggcgaag cgcgttggtt cgcccggtgc
     9421 gacgatcacg tggtaccgct tcagcgacaa gctggcgccc gccctgcaga cgaccggctg
     9481 acaaggagtc gagatggcag gaagtacaac gctgttcgac ctggacccgc tgaacccggt
     9541 tcaccgtgcg ctgatcgcct acggtctcgc gctagctgcc cgtcagcttc ccctgatgaa
     9601 tgccacatca acagcggccc ttcaagatgc ggtccgactg accggcatct cacaggacgt
     9661 gctgatgccg gcgctagctg aattccgcca agggttccag gctgagtacg agacgaaccg
     9721 acggaagcac aaggccaacc tcatggattc ggatgtgcgt tggtagttgc aggggtgcgg
     9781 gcggggcttc ggccccgccc gcgtcaccgt ttttgctatt attcccccat gaaagcatca
     9841 tttatcgata gagtcatctg gcccgacccc ggacattacg tggtggctgt gtccggcggg
     9901 gttgattcta tggcgctgct tgatttgtta gccgcgcatt ccgaa
//
LOCUS       DASVGB010000069         5487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_996866, whole genome shotgun sequence.
ACCESSION   DASVGB010000069 DASVGB010000000
VERSION     DASVGB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996866"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VF272_02020"
     CDS             complement(<1..1052)
                     /locus_tag="VF272_02020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7259684.1"
                     /translation="MNQAKDIYQRWRQLSHKQHIAIVSILVAAIIVLIPIATYAYFSN
                     AISSKENIMNRRDTGVTLLDRHNKPFFYLYQAKSKSTIAINTMPVHLKQALIATEDQD
                     YYKHPGFSLKAIAGAMYADIMQKELTYGGSTITQQLVKISLLTQNKNFLRKYQEIILA
                     AEIDRRYSKDEVLEMYLNSVYLGEGAFGVEAASQTYFGKPASALTLAEASLLIGILPA
                     PSAYSPVSGNADLAKKRQLLVLERMTEQKYITEEQEKHAAAIELHYRSRANGDTNRAH
                     HFAMMVKEELVKRYGEEKIARSGYKVTTSLDLDWQAEAETALRRQLTVLRFNEASNGA
                     VVALDPRTGEIRSLVG"
     gene            1104..1523
                     /locus_tag="VF272_02025"
     CDS             1104..1523
                     /locus_tag="VF272_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7259685.1"
                     /translation="MSTIKTQVNNASATEFIDKVDNETRRNDSFTLLEMFTRITGEKP
                     RMWGSSIIGFGQYHYKSERSKQEGDWPLTGFSPRKQNLTLYSMLGFGEYADLLKDLGK
                     HKTSKGCLYINTLSDVDLAVLENFIKQSYEEMKKQHN"
     gene            complement(1553..1792)
                     /locus_tag="VF272_02030"
     CDS             complement(1553..1792)
                     /locus_tag="VF272_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7259686.1"
                     /translation="MAIVITIDVMLAKRKMSVTELTDRVGITMANISILKNGKAKAVR
                     LSTLEAICKALNCQPGDILEYKSDEDSEITTLEKQ"
     gene            complement(1803..2267)
                     /locus_tag="VF272_02035"
     CDS             complement(1803..2267)
                     /locus_tag="VF272_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014114016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX7259687.1"
                     /translation="MNSGSTAILRGIVIVMGVIVLALCAFVLPPVISSDDAANYRPIL
                     LGLYVTAIPFFIALYQTLKLLRYIDMNKAFSDLSVQALRNVKYCAITISTLFAAGLPY
                     IFHVADKDDAPGVVLIGLVIAGTSFVIATSAAVLQKLLQSAIAIKSENDLTV"
     gene            complement(2286..3791)
                     /locus_tag="VF272_02040"
     CDS             complement(2286..3791)
                     /locus_tag="VF272_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX7259688.1"
                     /translation="MNRVNIKTQLVQAVAIVAVSTAFGILFDRFFYGKLPGLSFPLYV
                     AGLVGGIIGLAHYFHQKPSKAAWWLMIPLIGFATMVAVRASELLTLMNIAACLLLLLL
                     IAKVAFRRRLTDMRVKDFMSIPFLPLQFLGPARHTFGHIAAKRKVVKFQPRTAQIVKG
                     IALTVPVLVVFLFLFASADLIVQKYVNDLVSIEIDLELTARLVLIVLATLGFIGGYSY
                     LFSATKSDETPPETSLKPLIGKVESTILFVSVNTLFFAFILVQLAYLFGGEANISAQG
                     FTYAEYARKGFFELIAVAVVAFGLLWSAEKYIEKTERGHSTWFKFLSSALVLQVILIM
                     GSAFRRLYLYEQAYGFTTLRLYSHAFTVLLAVIFLILAYKIIRSASDQAFAFSALLAV
                     IVFLAALNAINQDAFIARQNLDRYHDTGKIDAVYLSQLSDDAVPVLVGGYSLLRPDDT
                     KILGAELNRRRLDARSDAARGWQAWNQSRAAADDALAGDASRLQLYPGNRN"
     gene            complement(3860..4303)
                     /locus_tag="VF272_02045"
     CDS             complement(3860..4303)
                     /locus_tag="VF272_02045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7259689.1"
                     /translation="MKIADSIHIDAPIEKVFGVFTDLESASARIKAITKLEIIDGPSS
                     MQVGTKWRETRTLFGKEATEVMWVTELVQNANYAVEAESHGTKYHSKYFFKQNGGATD
                     VEMVFEAEPVSTSARLMSFMGFLFAGATKKALHKDLEDLKAVCEK"
     gene            4494..4844
                     /locus_tag="VF272_02050"
     CDS             4494..4844
                     /locus_tag="VF272_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEX7259690.1"
                     /translation="MTNHRIYTISFASVYPLYVAKAKKKGRTKTEVDEIIHWLTGYGQ
                     EELEKQLEKQADLETFFAEAPQSNPSRALIKGVICGVRVEDIEEPTMQEIRYLDKLID
                     ELAKGRAMEKILRK"
     gene            complement(4859..5227)
                     /locus_tag="VF272_02055"
     CDS             complement(4859..5227)
                     /locus_tag="VF272_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7259691.1"
                     /translation="MEMKIELIPIPVADVDLAKAFYVDKVGFIADYDEVVSDTLRFVQ
                     LTPPGSACSIVLGVGITDMKPGSVKSYQMVVKNVAAAREELTKRGAEVSEVDVQPWGS
                     FVYFSDPDGNGWSLQELPPK"
     gene            complement(5283..>5487)
                     /locus_tag="VF272_02060"
     CDS             complement(5283..>5487)
                     /locus_tag="VF272_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7259692.1"
                     /translation="VACEDQEEIDYYWEKLSAVPESEQCGWLKDKYGLSWQIVPKNVD
                     ELLKRPGAYANMMQMKKLVIADY"
BASE COUNT         1528 a         1278 c         1221 g         1460 t
ORIGIN      
        1 ctgcctacca gagaacgtat ctcccctgtt cgagggtcta atgccaccac cgcaccgttt
       61 gaggcttcgt taaagcgcag caccgtcaat tgacgccgca acgcggtctc agcttcggcc
      121 tgccaatcaa gatcaagtga agtcgttacc ttatagccgg atcgagcaat tttttcctcg
      181 ccatatctct tcaccagctc ttccttcacc atcattgcga aatgatgagc cctattggtg
      241 tcgccgttgg ctcttgatcg gtaatgcagt tcgattgccg ccgcatgctt ttcttgctct
      301 tcagtgatat atttttgctc tgtcattcgc tctagcacta acaattgccg cttttttgcg
      361 agatcggcat tacccgacac cggcgagtag gctgaaggag ccggcagaat tccaattagc
      421 aaggaagcct ccgccaacgt aagcgccgag gctggtttgc caaaatatgt ttgagaggcc
      481 gcttctacgc caaacgcgcc ttctcctaaa tacacagaat taagatacat ctccaagact
      541 tcgtctttcg aatagcgcct atcgatttcg gcagccaaga tgatttcctg atatttgcgt
      601 aaaaagtttt tattttgggt taaaagcgat atttttacca actgctgcgt aatcgttgag
      661 ccgccatacg ttaattcttt ctgcataata tcggcataca ttgccccggc tatagcttta
      721 agggaaaatc ccgggtgttt atagtaatcc tggtcttcgg ttgctatgag ggcctgctta
      781 agatgtaccg gcattgtatt gattgctatt gttgatttgc tttttgcctg atagaggtag
      841 aagaacggct tattatggcg atcgagaagt gttacccccg tatcacggcg gttcataata
      901 ttttctttac tgctaattgc attggaaaaa taggcatagg ttgcaatcgg aatcagaacg
      961 atgatggcgg ccaccagaat tgagactatc gctatatgct gcttatgtga cagctgtcgc
     1021 cagcgctgat agatatcttt tgcttggttc atggagagat tataccctac tgtacttatt
     1081 cgcatggctg gtataattat tgtatgagta cgatcaagac ccaagtgaac aatgctagcg
     1141 caaccgagtt tatcgataaa gtagataatg aaaccaggag aaacgatagc ttcacgctgc
     1201 tggaaatgtt taccagaata accggcgaga agcccagaat gtggggctca agcattattg
     1261 gatttggcca ataccactac aaatccgaac gaagcaaaca agagggagat tggccgttaa
     1321 caggattttc tccgagaaaa cagaacttaa ccttatatag tatgcttggg tttggcgagt
     1381 atgccgattt actgaaagac ttgggcaaac ataaaaccag caagggttgt ttatatatta
     1441 atacgttgag cgatgttgat ttggctgttt tagaaaactt tatcaagcaa tcatatgagg
     1501 aaatgaaaaa acagcacaac taaatgacta tctacattta gttgccatgg atctactgct
     1561 tttctaaggt agtaatttcc gaatcttcgt cacttttata ctccaaaata tctccaggct
     1621 ggcagtttaa agctttgcag atcgcctcta acgttgataa ccgaacggct tttgcctttc
     1681 catttttcaa tatggaaata tttgccatgg ttattccaac tctatcggta agttccgtga
     1741 cgctcatttt ccttttagcc agcatcacgt caatagtaat tacaattgcc atgttcgtta
     1801 cctcagactg ttaggtcgtt ttctgattta atagctatgg cactctgcag taacttttga
     1861 agcacagccg cagaggttgc aatcacaaac gaagtaccag caattaccaa cccaataagg
     1921 accactcccg gagcatcgtc cttatcggcg acgtggaata tgtatggcag gcctgctgca
     1981 aataaagtac tgattgtgat tgcacagtat tttacatttc tcaaagcttg taccgataaa
     2041 tccgagaaag ctttattcat atcgatgtag cgtaatagtt tcaatgtttg atataacgcg
     2101 ataaaaaatg gtatcgccgt tacatagaga cctagtaaaa tcggacgata gtttgctgca
     2161 tcatccgagc taatgacagg cggtagtacg aaagcacaca aagcaagtac tatcaccccc
     2221 ataacaataa caattcccct taagatagct gttgatccgc tgttcatagc taaaaccttc
     2281 ggttgttaat ttcggttacc tggatatagt tgaagccgcg atgcatcgcc ggcaagggcg
     2341 tcatccgccg ctgccctcga ctgattccat gcctgccaac cgcgagcggc atcggaccgc
     2401 gcatcaagcc ggcggcggtt caattccgct ccaaggatct tggtgtcatc gggtcgtaac
     2461 aacgaatatc cgccaaccag caccggcact gcatcatccg atagttggct cagatatacc
     2521 gcatcaattt taccggtatc atggtaacga tcaaggtttt ggcgcgcgat aaaggcatct
     2581 tgattgatgg cgtttagggc cgccagaaat acgataacag ccaatagcgc agaaaatgca
     2641 aacgcctgat cggaagccga acggatgatc ttgtaagcca ggataagaaa tatcactgcc
     2701 aggagcacgg taaatgcatg gctatagagt cgtagcgtcg taaatccgta ggcttgctca
     2761 tataaataca aacgcctgaa agccgagccc ataatgagta taacttgcag cactagcgcg
     2821 ctactaagga acttaaacca cgttgaatgc ccgcgctcgg tcttttcaat gtacttctcg
     2881 gctgaccaca ataagccaaa tgccaccacg gcaaccgcga tgagttcaaa aaagcctttg
     2941 cgcgcgtatt cggcataggt aaacccctgt gccgagatat tcgcctctcc gccaaacaga
     3001 tacgcaagct gtacaagaat aaacgcgaaa aacagcgtat ttacagacac gaaaaggatg
     3061 gtgctttcaa ccttgccaat aagtggcttt aaagaagttt caggtggcgt ttcgtcggat
     3121 ttggtagcac tgaacagata ggagtacccc cctatgaacc cgagcgttgc tagcacaatc
     3181 aaaacaagcc ttgccgtgag ttcaagatca atttcaatgc tgaccaaatc attgacgtac
     3241 ttttggacga taaggtcggc cgatgcgaat aaaaacaaga atacgacaag aaccggcacg
     3301 gtaagtgcaa tgccttttac gatctgggcc gttcttggtt ggaatttcac gaccttacgc
     3361 ttcgccgcaa tgtgcccaaa agtatgtcgc gccggcccca gaaattgcag tggcagaaaa
     3421 gggatactca tgaagtcctt cacgcgcatg tcggtaagac gccggcggaa cgctactttg
     3481 gcaataagca atagcagcaa taaacatgca gcaatattca taagtgtcag caactcgctc
     3541 gcgcggacgg ctaccatggt ggcaaatccg atgagcggga tcatcagcca ccaagcagct
     3601 ttacttggtt tttggtgaaa ataatgtgcc aggccaatta tgccgcccac caagccagct
     3661 acgtatagag ggaaagatag tcccggtaat tttccataga aaaaccggtc gaacaatatt
     3721 ccgaaggcgg ttgacacggc gactatcgcc acagcttgaa cgagctgagt tttaatatta
     3781 acgcggttca taagctgtct ctcttagtat agccgcatta tacaatatta atttattgta
     3841 aatcaacaaa ttacgtaggc tatttttcgc acacagcttt taggtcttca aggtctttat
     3901 gaagcgcttt tttagttgca ccggcaaaaa gaaagcccat gaaactcatt agtcgggcac
     3961 tcgtactaac cggttcggct tcaaaaacca tttcgacatc agtggccccg ccattttgtt
     4021 taaagaaata tttgctatga tacttggtgc catgcgattc cgcttccaca gcataattgg
     4081 cgttttgtac aagttccgta acccacatta cctctgtcgc ttctttcccg aacagtgtgc
     4141 gtgtttcgcg ccacttagtg ccaacttgca tactcgacgg tccatctatg atttcaagct
     4201 tggtgatggc cttgatacgc gcagatgcgc tctccagatc cgtaaagaca ccaaatactt
     4261 tttcgattgg tgcgtctatg tgtattgaat cggcaatttt cataggtata gtatgctacg
     4321 tttaatgtaa tatagccaac gtagcgagtt tacgattcta tacatgtggt ggctggggct
     4381 agtggataaa ctccgagcag catatattac atgtaataat tatggggtag tcagatgtgt
     4441 agaaatataa aggggctcta taatttcgat ccgcccgcca atgaggcagt atcatgacca
     4501 accaccggat ctatacaata agcttcgcga gcgtctaccc cctttatgtc gctaaggcaa
     4561 agaaaaaggg acgaacgaaa acagaagtag atgaaatcat tcattggcta acaggatatg
     4621 gtcaggagga gttagaaaag cagttggaaa aacaagcgga ccttgagacc ttttttgcgg
     4681 aagctcctca gtctaatcct tcgcgggctt taatcaaagg ggtgatttgc ggtgtccgag
     4741 ttgaagacat agaagagccg accatgcaag aaattcgcta cctggataag ctaatcgacg
     4801 agttagccaa gggaagagcg atggagaaga tattgcggaa gtagtagctc ctcataccct
     4861 attttggtgg cagctcctgc aaagaccagc cgttaccgtc ggggtcagaa aagtatacaa
     4921 aggaacccca tggctgaaca tcaacctcac ttacctccgc tccccgctta gtcaactctt
     4981 cgcgagcggc agcaacattt ttaaccacca tttgatagct tttgacggat ccgggcttca
     5041 tgtctgtaat gccaacacca agcacgattg aacatgcgga acctggcggg gttagttgca
     5101 cgaaccgtaa tgtatcgctt acaacttcgt cgtagtcagc aatgaaaccg accttatcga
     5161 cgtagaaggc tttggccaga tcaacatcgg ctaccggaat gggtataagt tcaattttca
     5221 tttccattgc cgcataatag caaatggatt ttgttgatgc atctacgaaa atgccaaatt
     5281 cgttagtagt ctgcgataac cagctttttc atctgcatca tgttggcata ggctcccgga
     5341 cgcttcagga gctcatccac attttttggt actatctgcc aactgaggcc atacttatct
     5401 tttaaccagc cacattgttc ggattcagga actgccgaaa gcttttccca atagtagtcg
     5461 atttcttcct gatcttcaca ggcaacc
//
LOCUS       DASVGB010000070         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1185145, whole genome shotgun sequence.
ACCESSION   DASVGB010000070 DASVGB010000000
VERSION     DASVGB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185145"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VF272_02065"
     CDS             complement(<1..1584)
                     /locus_tag="VF272_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX7259693.1"
                     /translation="MKITPTSLTISQLLSSNKEQFVVPAYQRRYSWQVKHVAELYDDI
                     THLEIGETHLLGSIVCLTTSLVAGINTLELVDGQQRTTTIMLVLNAIEEKYKSMGEEK
                     EAEKLEGYLTCTDFDRSQKNKLVLGDLDETDFTKIMSGKKLDEVENRHLLEAHKLIYS
                     LLDKLGVEELDIFKDKFINQANVIRLDVAEAKDAFKLFETINNRGLSLSPADIIKNFL
                     LGNASTIDDETLEDVKQDWTQLIKNMDGLNLDDFFRQYMNGLLGRKVSFTGLIDSFKK
                     YYVDNVRNGDTLTGMTEYSPEVTDTILREELSVSDEEETVVIDVAHPSPQQETISITE
                     FVERLRKASDTYSKILNARFGDTKVDRHMINLQKIRSFASYTFVLNLMQRDIDRKEKF
                     EILRLLEIFMLRRNIAEYRTAELDDIFSSLTAIPDENIVETVKTTLRAYLPNDAEFEE
                     KLFMHDFRGRFENRAKYILETIEDHITGNTGEKYISDGEDVHLEHIMPQTITTKKAKE
                     EYGDWERYLGDEVDLHKTMV"
     gene            complement(1599..>3483)
                     /locus_tag="VF272_02070"
     CDS             complement(1599..>3483)
                     /locus_tag="VF272_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX7259694.1"
                     /translation="NNDPGYIELTNHSVLRVDESIGGNNDAIYKEMIRETIKEHLKKE
                     QMLRDRGIKVLSLFFIDKVANYLEYNEQGEELEGQFAAWFDELLIEERDKSEVYRDLL
                     PYSPSDLRKAYFSVMKKGGKNRAVDSSERGNESDYDAYDLIMSQKERLLSLDEPVRFI
                     FSHSALREGWDNPNVFQICVLREMGATLERRQTIGRGLRLPVDSNGERVQEPGIAQLT
                     VIANESYRAFADSLQKEYKAAGVSIGFVRKEEFSKLPALDTADLVPIGYNASLTIWEH
                     LLKTGMIDNDGRVTANFMPSNASFTLHLPENMKPYESLVVEIIQNCKIEKYVKQARQR
                     KKRTLNKAVVLSPEFQKLWERISKRSTYRVQVSRDDIIGMSVEAIKQEPQIDPIRIDI
                     TRSGLKVLRGGAKGEQLGVRSAEISGSYDLPDIIKELQQTTSLTRKTIVDILIASEKL
                     QEFIHNPNDFIQMAKRCIANVLATMVINGIQYEEIGGCVYELRELQKDGEQERESFLD
                     QMYVVKKQDKTLFDYVVYDSDVELQFAELLDSREDVKLFIKLPDRFKIPTPVGDYNPD
                     WAIVKQENGQEKIYMIRETKSTQQGNLLRPSEQAKIDCGKKHFTAIGIEDYAKASPDN
                     WNL"
BASE COUNT          855 a          851 c          729 g         1048 t
ORIGIN      
        1 taccatggtt ttatgcagat caacctcatc tcccaagtag cgctcccaat ccccgtactc
       61 ttccttggct tttttggtgg tgatcgtttg tggcataatg tgctctaagt gaacgtcttc
      121 gccgtcgcta atgtattttt caccggtatt gccagtaata tgatcttcaa tcgtttccaa
      181 aatatacttt gctcgattct caaaccgacc ccggaaatcg tgcatgaaaa gcttttcttc
      241 aaattcagca tcatttggca ggtaggcccg caaggttgtc tttaccgttt ccacaatgtt
      301 ttcatcagga attgcggtaa gacttgaaaa tatgtcatca agttcggcgg tgcgatactc
      361 agcgatattg cgtcggagca taaaaatttc aagcaatctc aaaatttcga atttctcctt
      421 acgatcaatg tcgcgctgca taagattcaa cacaaaggtg tacgaggcga acgaacgaat
      481 cttttgtagg ttgatcatat gacgatcaac cttagtatct ccaaaacggg cattgagaat
      541 cttgctatag gtatcagatg ccttgcgtag ccgctcaacg aactcggtta tggaaatggt
      601 ttcttgttgg gggctaggat gcgccacatc aattaccacc gtttcttcct cgtctgagac
      661 cgagagctcc tcccgcaaga tagtatctgt tacttctggc gaatactctg tcatgccggt
      721 aagggtgtcg ccgttcctga cattatcgac gtaatatttt ttaaagctat caatcaagcc
      781 ggtaaatgat accttgcgtc ccaataagcc gttcatatat tggcgaaaga agtcgtcaag
      841 gttcagtcca tccatattct tgatgagctg cgtccagtct tgcttcacat cttctaacgt
      901 ctcatcatca atcgttgagg cgttcccaag caagaagttc ttaataatat cggcaggact
      961 caggctgagg ccacggttat taatggtttc aaataatttg aaagcatcct tggcctcggc
     1021 tacatccaaa cggatcacat ttgcttgatt aataaatttg tctttaaaga tatcaagctc
     1081 ttcaacgcct agcttatcga gaagtgaata aataagttta tgcgcctcca gcagatgtcg
     1141 attctccacc tcgtccaact ttttaccgct cataatttta gtaaagtcgg tctcatccag
     1201 atcgcccagc accagcttat tcttttggct gcggtcgaaa tcggtacagg ttaagtagcc
     1261 ttcaagtttt tcagcctctt tttcctctcc catcgattta tatttttctt cgattgcatt
     1321 caaaaccagc ataatagtcg tggtgcgttg ctggccatca accagttcaa gggtgttaat
     1381 gccggctacc agactggtgg ttaggcagac aatgctaccg agcaagtgag tttcacctat
     1441 ttcaaggtgt gtaatatcgt cgtagagctc ggctacgtgc ttcacctgcc acgaatagcg
     1501 gcgttggtag gctggaacca caaactgctc cttattactg ctgagaagct gcgagattgt
     1561 tagggatgtc ggggtaattt tcattgtgct attgtaccct ataaattcca gttgtcagga
     1621 cttgccttgg catagtcttc aataccgatg gcggtaaaat gcttttttcc gcagtcaatc
     1681 ttggcttgtt cggaaggccg caagaggttg ccttgttggg tacttttggt ttcgcgaatc
     1741 atataaatct tttcttggcc gttttcctgc tttacgatgg cccaatctgg attatagtca
     1801 ccaacgggtg tgggaatttt aaacctatca ggtaatttta taaacagctt gacgtcttcg
     1861 cgagaatcta gcagctcagc gaactgcagc tcaacatccg aatcataaac cacgtaatca
     1921 aataaggttt tatcctgctt cttaaccaca tacatctgat cgagaaagct ttcacgctcc
     1981 tgctccccgt ccttttgaag ctcccgcagc tcatagacgc agccgccaat ttcctcatac
     2041 tgaatcccat tgattaccat agttgctaac acgtttgcta tgcagcgctt ggccatttga
     2101 ataaagtcgt tcggattatg aataaattct tgaagcttct cagaggcaat gagtatatca
     2161 acgatggttt tgcgggtaag gctggttgtt tgctgtagct ccttaataat atcgggcaga
     2221 tcataactac cgcttatctc ggctgaacgc acgcccagct gctcaccctt ggccccgccg
     2281 cgcagtactt taagacccga ccgggtaata tcaatgcgaa ttgggtcaat ctgcggttcc
     2341 tgcttaatgg cttccaccga cataccgatg atatcgtcgc ggctaacttg cacgcgatag
     2401 gtagaacgtt tgctaattcg ctcccataat ttctgaaact ctggcgacaa gactactgct
     2461 ttatttaagg tacgcttttt ccgttgccgt gcctgtttta catatttttc gattttgcag
     2521 ttttggatga tttcgacaac caatgactca taaggtttca tgttttccgg aagatgcaag
     2581 gtaaatgaag cgttgcttgg cataaagttt gcagttaccc ggccgtcatt atcaatcatg
     2641 ccggtcttga gcaaatgctc ccaaatggta agtgaggcat tgtatccaat tggcaccaga
     2701 tcggcagtat caagggcggg aagtttacta aactcttctt ttcgaacaaa tccaatgctc
     2761 acaccggcag ccttgtattc tttctgtaaa ctgtcggcaa atgctcggta tgattcattt
     2821 gcgatcaccg ttaactgggc aattcctggt tcctgaactc gctcgccatt actatctacc
     2881 ggtaaacgca aacctctgcc aatagtttgc cgccgctcaa gcgtggcacc catttcgcgt
     2941 aggacacaga tttgaaacac attgggattg tcccacccct cacgtaaggc ggagtggcta
     3001 aaaataaacc gtaccggctc atccaggctc agcaggcgct ctttctggct cataatcaag
     3061 tcgtaggcat catagtcgct ttcattgcca cgttcactgc tgtctactgc gcggtttttc
     3121 ccaccctttt tcataactga aaagtaggcc ttgcgtaaat ctgaaggact gtagggtagc
     3181 aggtcgcgat atacctcgga cttatcacgt tcttcaataa gcagctcatc gaaccatgca
     3241 gcaaactggc cttccagctc ctccccttgc tcgttgtact ctagatagtt tgcaacctta
     3301 tcaatgaaaa acaggctgag taccttaatg cctcgatcgc gcagcatctg ttctttcttc
     3361 agatgttcct taatggtttc ccgaatcatc tccttatata ttgcatcgtt gttgccgcca
     3421 attgactcgt ctactcgcaa cacgctgtga ttcgtcagct caatatagcc aggatcattg
     3481 tta
//
LOCUS       DASVGB010000071         6330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_646531, whole genome shotgun sequence.
ACCESSION   DASVGB010000071 DASVGB010000000
VERSION     DASVGB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646531"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="tuf"
                     /locus_tag="VF272_02075"
     CDS             <1..1083
                     /gene="tuf"
                     /locus_tag="VF272_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7259695.1"
                     /translation="LATRLPSDVNKSVAYDQIDNAPEERQRGITIATSHQEYESEKRH
                     YAHVDMPGHADYVKNMITGAAQIDGAILVVSAADGPMPQTREHILLARQVGVPYILVF
                     MNKMDMADPELAELVEIEIRELLEKQGYDENAPIIKGSALKALEGEAAAEETIMELVK
                     ALDESVPEPKREIDKPFLMPIEDVFSIKGRGTVATGRIEQGKVAVGDEIEIVGIRDTK
                     KTTVTGVEMFKKLLPDAQAGDNVGALLRGIERDDIERGQVLAKPGSITPHTEFDSEVY
                     VLKKEEGGRHTPFFKGYKPQFYIRTTDVTGEIQLPEGVEMVMPGDNIQMKVKLIAPIA
                     MHDGLRFAIREGGRTVGAGVVTKITK"
     gene            1094..1420
                     /gene="rpsJ"
                     /locus_tag="VF272_02080"
     CDS             1094..1420
                     /gene="rpsJ"
                     /locus_tag="VF272_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7259696.1"
                     /translation="MTKDDAAPKQRIRIRLKAYDNKVIDQSAKQILDTATRTGAKVAG
                     PIPLPTDRKLQTVIRSPHIFKKSREQFEMRTHKRLLDITEPTPKTVDSLMNLSLPAGV
                     DIEIKM"
     gene            1485..2006
                     /locus_tag="VF272_02085"
     CDS             1485..2006
                     /locus_tag="VF272_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259697.1"
                     /translation="MDLPQAIQYISAIIVIVGAGILLGLSLRRRQPLQSAHSQHERLQ
                     TYAELIGAMDGLRTANEDHKGDFISAYYRALVYAPDSVVEALNAFLQTVTISEKQYDS
                     VKQTAARDAAALEMRRDAQHQLDSSTRLQPGNLYSIEVNTQESDSPASVMKPESRVDT
                     APLLAEAEAKNAQ"
     gene            2063..2530
                     /locus_tag="VF272_02090"
     CDS             2063..2530
                     /locus_tag="VF272_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259698.1"
                     /translation="MARKPKRRVKRNSGGRQELVRSITERTWYELPDWATAWLAKYAG
                     RVVFGMVILLSPIAILAVVLGLHALPLHFIGIAGSDNALGLKAVLFLITYGVLAFSVK
                     PLMRGLNRGWWLVILAGAVNAINNAVAGEGGLSGIILLALAGYLFTQVRHRFD"
     gene            2585..3790
                     /locus_tag="VF272_02095"
     CDS             2585..3790
                     /locus_tag="VF272_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7259699.1"
                     /translation="MSLFDAPQQPLADLLRPKHVDEVIGQRHLVGTGQFIRQLIESGQ
                     PVSLILWGPPGSGKTTLARIIAGSSAHAFEELSAAIAGVADVRKVIERARERRKMGQG
                     TILFVDEIHRFNKSPQDAFLPHVENGTIILIGATTENPSFEVVSPLLSRSRVVTLNPL
                     SQQELLKLLERAMQRYKGKELQPKAAALLTELAAGDGRVALGGLEAAASLGDIITPES
                     IKQAMQKTSLRYDKGGEFHYNLISAFIKSMRGGAVDAALFYLARMLEAGEDPKFIARR
                     IVIFASEDIGAADHSMLQLAVATFQAIERIGMPEGRIILSQATIGMATAKKNRDAYDA
                     IGAAQQAVRDNPATEPPLHIRNAPTKLMKDLGYGKGAKWEPGFEHPQGFMPEGLEDLT
                     FYKPQKPGK"
     gene            3823..4071
                     /locus_tag="VF272_02100"
     CDS             3823..4071
                     /locus_tag="VF272_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259700.1"
                     /translation="MASNKPPANEYDSVYFLKVLLYLLLGTVWIAFDEKKYIPAGIII
                     GILIAQHERLQLDRKIEYAMLIVGGLLGLVGIGLTFWL"
     gene            4184..4729
                     /locus_tag="VF272_02105"
     CDS             4184..4729
                     /locus_tag="VF272_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259701.1"
                     /translation="MTDSTLDDECDAALWIDFRAVSEGQAARRKHADHHEILRKLLRA
                     ELVIHNSRREDRHSLQLVDYWDDMEDRCRRALQALNEGCTETARLFADGFKPKVIVFK
                     STGFLVTSDNVVKAFAPAMALTWILLQVGAPLIPIVVLTWVGAVMLARRWSADALKPP
                     PEIPLDDPDPWVQDSPPKRRR"
     gene            4966..5592
                     /gene="rplC"
                     /locus_tag="VF272_02110"
     CDS             4966..5592
                     /gene="rplC"
                     /locus_tag="VF272_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7259702.1"
                     /translation="MKALLGKKLGMTQLFDENGNVSRVTIVEAGPCVITQLKTNDTDG
                     YLAVQFGFESAKHPAKPQVGHATFKDVTPKHFREVRLDKVGAKQSDEEMKVGDTWKAD
                     RFEIGDAVEVVGTSKGKGFAGTIKRHNFHRGPKTHGSHNYRAPGSIGSGYPQHVLKGQ
                     KMAGRMGGDQVTVKGLKIVHVDAEKNLLAISGAVPGPRKSLVMIREAR"
     gene            5592..6311
                     /gene="rplD"
                     /locus_tag="VF272_02115"
     CDS             5592..6311
                     /gene="rplD"
                     /locus_tag="VF272_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX7259703.1"
                     /translation="MAQAVLYSKTGTKKETAVKLPAAVFGTEPNHSLISAAYETYVAN
                     GRHAGAKTLTRGEVRGGGKKPWKQKGTGRARAGSSRLPHWTGGGVAFGPVGLENHSKS
                     MPVKAKRAAIRGALSLQATDQKIMVIEDFVAPDGKVSATVKLLNKLEVEGSIVLVVAT
                     KDTLIDRATRNMPGVNVVSATYLNVFTILNADAIIMTQAALEQVEAWLGAADSPAAKQ
                     PEAKKPAAAKKPTAKKAEAKA"
BASE COUNT         1596 a         1493 c         1721 g         1520 t
ORIGIN      
        1 ctggctacac ggcttccaag cgatgttaac aaatctgttg cttacgatca gatcgacaac
       61 gcaccagaag agcgacagcg tggtattact attgcgacct ctcaccagga atacgaaagc
      121 gaaaagcgtc actacgctca cgttgatatg ccgggacacg ccgactatgt gaagaacatg
      181 atcaccggtg ccgctcaaat tgacggagcc attctggtag taagcgccgc cgatgggccg
      241 atgccgcaaa cccgcgagca cattctgttg gctcgccaag ttggcgttcc ttacatcttg
      301 gtcttcatga acaagatgga catggctgat ccggagctgg ctgaactggt tgaaattgag
      361 attcgcgagc tgcttgaaaa gcagggttat gatgaaaatg ctcctattat taagggatca
      421 gcccttaaag cgttggaagg cgaagccgct gctgaagaga ctatcatgga actggttaag
      481 gctctcgatg agtcggttcc agaaccgaag cgcgagatcg ataagccttt cttgatgcca
      541 attgaagatg tcttctcaat taaaggtcgc ggtacggttg ctaccggccg tattgagcaa
      601 ggtaaggttg cagtaggtga tgaaattgaa atcgtcggta tccgcgatac caagaaaacc
      661 accgttaccg gcgttgagat gtttaagaag cttctccctg atgctcaagc cggcgataat
      721 gtcggtgctc tgcttcgcgg tattgagcgc gatgacattg agcgcggtca ggtattagcc
      781 aagccgggtt caatcacccc gcataccgag tttgactctg aagtctatgt actgaagaaa
      841 gaagaaggcg gacgccacac cccgttcttc aaaggctata agccgcagtt ctacattcga
      901 accactgatg ttaccggaga aatccagctt cctgaaggcg ttgagatggt aatgccgggc
      961 gacaacatcc agatgaaggt taagctgatc gccccgattg ctatgcatga tggtttacgc
     1021 ttcgccattc gtgaaggcgg ccgcaccgtt ggtgccggcg ttgtaaccaa gatcaccaaa
     1081 taacggacat cacatgacca aagacgacgc cgcacctaaa caacgaatcc ggatccgctt
     1141 aaaagcttac gacaacaagg ttatcgacca gtcagctaag cagattttgg ataccgctac
     1201 acgaaccgga gccaaggtgg ccgggccaat tccgttacca accgaccgaa agcttcaaac
     1261 cgttattcgc agcccgcaca tcttcaagaa gtctcgcgag cagttcgaga tgcgaacgca
     1321 caagcgcttg cttgatatta ccgaacctac ccctaaaacc gttgactcac tgatgaacct
     1381 cagtttgcca gccggagtcg atatcgaaat caaaatgtaa gcgcctatgc ttgcgattcc
     1441 tacctgctgg gtggacaatg ggccatacaa caggagtaca gactatggat ttaccacagg
     1501 ccattcaata tatttcggcg attattgtaa ttgtgggggc cggcattttg ctaggcttgt
     1561 cactgcgccg gcgccaaccg ttacaatcgg cgcattctca gcatgagcgg ctgcaaacct
     1621 acgccgaact tattggtgcc atggacggtt tgcgcacagc caacgaggac cataaaggtg
     1681 attttatttc cgcctattac cgtgctcttg tgtatgcgcc ggacagcgtg gttgaagcgc
     1741 ttaatgcctt tcttcaaacc gttacaatct cggaaaagca gtacgactct gtcaagcaga
     1801 ccgcagcccg cgatgcggcg gctctggaaa tgcgccggga tgctcaacat cagcttgact
     1861 catcaacgcg tttgcaaccg ggtaatttgt acagtattga ggttaatacc caagaaagcg
     1921 atagccctgc gagcgtgatg aagcctgaat ctcgtgttga taccgcaccg cttttggctg
     1981 aggccgaagc caaaaacgct cagtaacgtt gtcggatggt agcataagcg ctatagtaaa
     2041 gttataaaga atatgcagac ttatggcacg aaaaccgaag cgtcgagtaa aacgtaattc
     2101 tggcggtcgt caggagctgg tgcggtcgat tacggaacgt acgtggtacg aactgcctga
     2161 ttgggctacg gcctggctag cgaaatatgc cggccgcgtt gtgtttggca tggtcatatt
     2221 gctgtcgccg atagcaatat tggcggtagt cttaggcctg catgcgctgc cattgcattt
     2281 cattggaatc gccggaagcg ataatgccct ggggttgaaa gcggtgcttt ttcttataac
     2341 atatggcgtg ctagccttct ccgtaaaacc gttaatgcgc ggtttaaacc gggggtggtg
     2401 gctggtgatt ctagcgggtg cggtaaatgc cattaataat gcggttgccg gtgaaggcgg
     2461 ccttagcggc attattttat tggcattggc cggatatctc tttacgcaag tacgccaccg
     2521 tttcgactag ctagggaagc gctatgtttg gattttgttc gtttaactag atataatacc
     2581 gtctatgagt ttgtttgatg cgccgcagca accgttagcc gatcttttac gtcccaagca
     2641 cgtcgatgag gttattgggc aacgccacct ggttggtacc ggccaattta ttcgccagct
     2701 tattgaaagc ggccagccgg tatcgttaat tctatggggt cctccaggca gcggtaaaac
     2761 aacgctggct cgcattatcg cgggcagcag cgcacatgca tttgaagagc taagcgctgc
     2821 aatcgccggg gtagccgatg tgcgcaaggt aatcgagcgg gcacgagagc ggcggaagat
     2881 ggggcaaggt accattttgt ttgttgatga aatccatcgt tttaataaat ctccacagga
     2941 tgcattttta ccgcatgttg aaaacggcac cattatttta attggggcga ctacggaaaa
     3001 cccctcgttt gaagttgttt cgccgctgct gtcgcgcagc cgggttgtaa cgcttaaccc
     3061 tttgtcgcag caggaactgc taaaactgct tgagcgggcc atgcagcggt ataaaggaaa
     3121 agaattgcag ccaaaagcgg cggcattatt gaccgaattg gccgctggcg acggcagggt
     3181 agcgcttggg ggattggaag cggcggcaag cttgggagat attatcacgc ctgaaagcat
     3241 taagcaggcg atgcaaaaaa ctagcttgcg ttatgataaa ggcggcgaat ttcattacaa
     3301 tcttatttcg gcgtttatta aatcgatgcg aggaggcgcg gttgatgcgg cattgtttta
     3361 tttagccaga atgcttgaag caggggaaga tccaaagttt attgctcgcc ggattgtaat
     3421 tttcgcgtct gaggatattg gcgctgccga tcattccatg ttgcaactgg cggtggcaac
     3481 atttcaggcg attgaacgca tcggaatgcc ggagggaaga attattctct ctcaagcaac
     3541 aattggtatg gcgaccgcaa aaaaaaatcg tgatgcctat gatgcgattg gcgcggccca
     3601 gcaggcggtg cgggacaatc cagccactga gcctccattg catattcgga acgcaccgac
     3661 caagttaatg aaagatttgg ggtatgggaa gggtgcaaaa tgggagcctg gttttgaaca
     3721 tccgcaggga tttatgccag aagggctgga agatcttact ttctataagc cgcaaaagcc
     3781 tggcaaatag atttctattc cgtttacgct tatactaggt gtatggcttc caataaacct
     3841 cctgcaaatg aatacgacag cgtttatttt ttaaaagtac tgctgtattt actcctcgga
     3901 acggtatgga tagcgttcga tgaaaaaaaa tatattccgg ctggcattat cattggaatc
     3961 ttaattgctc agcatgagcg cctgcagctt gatcgcaaaa tagagtatgc catgctgatt
     4021 gtgggcggtc tgctcggcct ggttggcatc ggtctgactt tttggttgta gtaggtctac
     4081 ttagtctgtt aaggtatgca gtcctattag acattgatat ttaattattt tatgttatta
     4141 taatctaacg tttttaacgc agtccaaacc aaaggatttt gacatgaccg atagcacgct
     4201 agacgacgag tgtgatgccg ccctgtggat cgactttcgt gccgtcagtg aaggacaggc
     4261 tgctcgtcgt aaacacgctg accatcatga gatcttgcgc aaactcctcc gagccgagct
     4321 tgtcattcac aactcaaggc gggaagatcg ccacagtctg caacttgtag actactggga
     4381 cgatatggag gatcgctgtc gccgtgctct tcaggctctc aatgagggct gcactgaaac
     4441 tgcccggctg ttcgctgatg ggttcaagcc caaggtgatc gtttttaagt ccacagggtt
     4501 cctagtgaca tccgacaacg tcgtaaaggc ttttgcgcct gcgatggccc tcacatggat
     4561 tttgctgcaa gtcggtgcgc cactcatccc gatcgtcgta ctcacgtggg tgggtgctgt
     4621 gatgttggca cgtcgctggt cggcggacgc cctgaaacct ccccccgaga tccctctgga
     4681 tgatcctgac ccttgggtgc aggacagccc gccgaaaaga cgtcgctgac tcgctgggtc
     4741 agcatcagcc gcatcaaaag gcttacggaa ggctcgcgcc cgccgtgagc cttttcgttt
     4801 gttgttggct cgggctattc gttgacagat agccatcagg tttggtatag tgccctggta
     4861 actcttgagc tgggccgcac gccactcacc tgggaatcag ataggatcgg ttgcatccga
     4921 taaccacctg aatcccggaa ttgttttttg ttaacaggac gtttgatgaa ggccttacta
     4981 ggaaaaaaac tcggtatgac ccagctgttc gatgagaatg gtaatgtgtc gcgcgttacc
     5041 atcgtggaag ccggaccgtg tgttattact caacttaaaa caaatgacac cgacggctat
     5101 ctggcagtgc agtttggctt tgagtcggcc aagcaccctg ccaaacctca agtcgggcat
     5161 gcaaccttta aagacgtaac gccgaagcac tttcgtgaag ttcgcctgga taaagtgggc
     5221 gctaagcaat cggacgaaga gatgaaggta ggagacacat ggaaggctga caggtttgaa
     5281 attggagatg cagtagaggt tgttgggacg agtaagggca aaggatttgc cggcactatt
     5341 aaacgtcata acttccatcg cggccccaag acccatggtt cacataatta tcgtgcaccc
     5401 ggctcgatcg gttcaggcta tccccagcat gtcctaaaag gtcaaaagat ggctggacgc
     5461 atgggcggtg atcaggtgac cgttaaaggt ttgaaaatcg tacatgtgga tgctgaaaaa
     5521 aatttactgg caattagcgg cgccgttccc ggtccgcgta aaagcctggt aatgattcga
     5581 gaggcacgct aatggcacag gcagttcttt attctaagac cggtacaaaa aaagaaacgg
     5641 ctgttaagct gccagcagcg gtatttggca ccgagccaaa ccatagttta attagcgcgg
     5701 cgtatgaaac ctatgttgcc aacggtcgcc atgccggagc caaaacgttg acacgcggtg
     5761 aagtgcgcgg tggcggtaaa aagccgtgga agcaaaaagg aacaggacgt gcacgggcgg
     5821 gttcaagccg gttgccgcat tggactggcg gcggcgtagc ctttggaccg gtcgggttag
     5881 agaaccattc aaaaagtatg ccggttaagg ctaagcgagc agccattcgc ggggcattga
     5941 gccttcaagc cactgatcag aaaattatgg taatagagga tttcgtagca cctgatggca
     6001 aggtgagtgc aaccgtgaag ttgctgaaca agcttgaggt tgaaggttca attgtgctgg
     6061 tagttgccac taaggatacc ctgatcgacc gagcgactcg taatatgccc ggtgtcaatg
     6121 tggtgagtgc cacctatctg aacgtcttta ctattctcaa tgcagacgcc atcattatga
     6181 cccaagcagc cttagagcag gttgaagcat ggcttggcgc agctgattcc ccagcggcca
     6241 agcagcccga agctaagaaa ccggctgctg ccaaaaagcc aactgctaag aaagctgagg
     6301 cgaaggcatg aatccggtag tacttcgtcc
//
LOCUS       DASVGB010000072        13800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_998193, whole genome shotgun sequence.
ACCESSION   DASVGB010000072 DASVGB010000000
VERSION     DASVGB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998193"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /gene="der"
                     /locus_tag="VF272_02120"
     CDS             <1..1293
                     /gene="der"
                     /locus_tag="VF272_02120"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7259704.1"
                     /translation="IGRPNVGKSSLFNRLVGVRQAITHETAGTTRDANYGSLTWNGKH
                     FILADTAGLYKADNDTLEMAVQEQITEIANLADVLVVVIDAGVMITKEDQLAARLARK
                     TGKPMVLAVNKIDTAQAGPVDPPEQLGIDPIVQTSAVHGRGTGDLLDAIAEHLAAVPE
                     PPFEADVVTLALLGRPNVGKSSLLNAMAGKQQAIVSERAGTTRDIGSIDITYHGKTIR
                     ILDTAGLRRRGKIEQGIEKYSSLRTLAAIARADIGVLVMDATEPSVAGDQHIAGAVVD
                     AGKGLILVTNKWDAVQDKDDKTQVRFTRKLISDFAFAHWAPLVYTSATQGLNVTQLFK
                     LTQEIALIRTKTVPTGELNRLMEKMIAKQAPAGLKNRQPKIKYATQTGTKPPTFTLFS
                     SYPEMVHFSYTRYLENGLREAFGFDGTPIKLEFRGKQK"
     gene            1525..2094
                     /gene="pth"
                     /locus_tag="VF272_02125"
     CDS             1525..2094
                     /gene="pth"
                     /locus_tag="VF272_02125"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX7259705.1"
                     /translation="MKLIIGLGNIGKEYHATRHNLGFGVVDMLAITFGKQPSDFTKHA
                     KAAAEILDLKSEFGCMLVRPTTMMNLSGQAVQALAHYYKIAPHNIWVIYDDVDLPFGH
                     MRVRLGGGSAGHNGIKSIMHHIGDEFWRVRLGIANDYLATTPTDKFVLDPFMAEEATK
                     VPALLETAANYIEAALLSGNLSDHSQHLH"
     gene            2397..3275
                     /gene="dprA"
                     /locus_tag="VF272_02130"
     CDS             2397..3275
                     /gene="dprA"
                     /locus_tag="VF272_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7259706.1"
                     /translation="MSTIFMQPSKKIAQVNYDNDQYPVILREIGSPPQRLYVRGELSR
                     APHIAIVGSRRPTAYGEQVTYMLASELAAAGICIVSGLALGIDGIAHRAALEAGGTTI
                     AVLGSGLNQVYPARNRGIAAQISAGRGAVVSEYEAAMPALPHHFPLRNRIIAGLSLGV
                     IVTEADASSGSLITADHALQANRTVMAVPGNITSLRSAGPHKLLRDGAIPITSAAEVL
                     NALNLDIASLSQSRPAPASQQEYIILSLLSQGVSNGRDLIEQSGLEPAVFARTISIME
                     ISGKVYNLGIGNWVRR"
     gene            complement(3272..4291)
                     /locus_tag="VF272_02135"
     CDS             complement(3272..4291)
                     /locus_tag="VF272_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259707.1"
                     /translation="MMPRYLYRNDRSGELLELFLVAAISSVLLVRFLLTLTGYPQLST
                     SELHVAHMLPGGVLMVAALVLTMAFLGNRVQRLASVIGGIGFGLFIDELGKYVTHDNN
                     YFFQPTVALIYLVFVIMFLMFRSLSNRQRRFSKEEHLLNALNMLEEAVINELDRPELK
                     RVLANLHRSDPNHPLVKPLEKALERIGAQKDSEPALIRRWLRQIESRYQKIMATTWAI
                     RLIDTVFALKAGIFLTDAVVDASRWYTQVPLQGFAVVSTLQFGASLISTGFVVAGIAI
                     IRSSRLRAYELFTKSLLIDIFVVQFFSFYQKEFSALPGFIASILLYIALRFLVRQERQ
                     IVHRT"
     gene            4389..4760
                     /locus_tag="VF272_02140"
     CDS             4389..4760
                     /locus_tag="VF272_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259708.1"
                     /translation="MNESLQIVPRITVEAKPGDRLVFCSRQDDVWVIKADLNPVGVGD
                     DPRIICWERPHEGVCWLVHITGGQLRLKEYRPGIRPLEWFIPFSFQPSGLLLQIIDGW
                     VPKVLRGRIDDALAKPPATAQ"
     gene            4871..5866
                     /locus_tag="VF272_02145"
     CDS             4871..5866
                     /locus_tag="VF272_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX7259709.1"
                     /translation="MNVWSQLHQAHKPFFIFAPMDDVTDTVFREVVARAAKPDLTMTE
                     FASSDGFAHPKGRPSVEQRLRVNDTEVELAVPLIAQIWGNHPDHYYAMAKDLAARQQF
                     AGIDINMGCPEKGIVKRGCCGGLIKAENWENAAAIIEATKAGAGNLPVSVKTRIGLGT
                     IITEEWVSHLLKQDIAALIIHGRTVREMSRVPAHWDEIGKAVKLRDQLAPKTLMIGNG
                     DVLSRNQGERLAAAYNLDGIMIGRGVFQDPFVFASKEAEHPPEEMFAILLHHVNLYDS
                     TWGDAKSYNPLKRFFKMYVSGFPGAAELRARLMDTKTPDEARKIMGRITPSGIVA"
     gene            6043..6273
                     /locus_tag="VF272_02150"
     CDS             6043..6273
                     /locus_tag="VF272_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259710.1"
                     /translation="MKGVDFIVRHNASVVFLIAADPQHQAQWAKSLLYRTCQRQMTAA
                     RKDRIDRALEVLEVSGTGLDPEHHDVFLIARR"
     gene            complement(6424..6867)
                     /locus_tag="VF272_02155"
     CDS             complement(6424..6867)
                     /locus_tag="VF272_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259711.1"
                     /translation="MYPLSIGRIEIMSLHHRLSEPPRMKPTRAQRLQIERLQRYLRQQ
                     DGFIYDPDDGVALHLAVHISELLPEEGDISAAEVEELLWVIRSSSKGEVVHLRGHNRK
                     TIGFALDHAAAKKMDRVIVSMMPLRHRLRRSWGRFWPSLHRPWKR"
     gene            7108..8238
                     /gene="metK"
                     /locus_tag="VF272_02160"
     CDS             7108..8238
                     /gene="metK"
                     /locus_tag="VF272_02160"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX7259712.1"
                     /translation="MTSNVFTSESVCAGHPDKVCDQISDAILDEVLRQDPNGRVAVET
                     AVTANRIILLGELTTTAKIDFVKVSREQVKRLGYTDPDFHFTPNCDVEVYIRSQSPEI
                     AAGVDLDGAGDQGMMFGYACHETKQLMPLPIMMAHSLTRAIDAARESGTLPYLRPDGK
                     AQVTIRYENDMPVGIEHITMAVPHNEEIERKQVHADLVTEVVLPVLAEYGLDMGSAKV
                     IVNGTGVWHAPGPASDAGLTGRKIIVDSYGGYARVGGGAFSGKDPTKVDRSGAYAARF
                     LAKNIVASGMADRCEVGLAYFIGAKQPVMHQINTFGTAKESQKAIAAFSTEILDTSVK
                     GIIAALDLRRPIYLDTAAYGHFGRDDVSWEKVVEHAKISANA"
     gene            8395..9906
                     /locus_tag="VF272_02165"
     CDS             8395..9906
                     /locus_tag="VF272_02165"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEX7259713.1"
                     /translation="MKWLTKSNYLHFLIHPAYFWLEKHDKEKLPPFDDAAQAIMDAGN
                     EIEEYARERFPLGQLVPGGLFDQIDESTKLLKHAAAGETLFQAAVLTDQKWYARCDVV
                     IKNNGGSWDIYEIKSATKVKPEHLHDVAFQKIALEECGHPVRNTYIVHISSHYVRQGA
                     VDSQKLFEQVDVTAAVDGLLHKTKRDIAAAQAMMALPECPSDSPALASNWYAWRDIYR
                     HLHPEITDLSIYNLARLKPEQLSALAKLGISDMLSIPDSFALGSQQKAQLQALRAGEP
                     IIHPVKIAAALAGLAYPLYFFDYETVGPGIPAYDGTKPYQQVPFQYSLHILDKPGGKL
                     RHTEFLATGNANPMPALLEQMQHDIGPIGSVIVWYKGFEMGVNDAMAAIYPEHAAFLH
                     QVNTRVFDLMEIFSNFHYVDAAFGGSASIKKVLPVVVPELTYSDLAIQKGDVASKLWE
                     QTAQGKITGKQAEKIYADLRIYCGQDTLAMVRIYEFLMRITKTAPGAQLSLLG"
     gene            9933..11213
                     /locus_tag="VF272_02170"
     CDS             9933..11213
                     /locus_tag="VF272_02170"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7259714.1"
                     /translation="MKQPQVSAVIGGQWGDEGKGKIVDYLAADADVVIRSQGGDNAGH
                     TVLNERGKFGLHLVPAGIFNPDTINIMGAGVALNPKTLLKEMKELGERGVSFDNLIIS
                     PKAHLAFDYHLFIDQSQEHARGKNRIETTKRGIGPTYMDKAERIGLQAKLLLEPDRCM
                     EELARVLALKRKHLDQDNEVLYPEYYEDFVKEAGAALKDFIRPTERIIEEHLHKGSRF
                     VLEGAHGTLLDLDHGTYPMVTASNSTVGGLLVGAGMPPLALNKVVGIFKSYQTRVGAG
                     GMPTELDNELGEHIRTRGHEFGTTTGRPRRVGFFDGVAALYAHRINGFTDIAVTRIDT
                     LADVKELKIATGYKDTAGWPTDFPTDNTRLSDCIPAYESYEGWTEAEIAKASTFDELP
                     LSAKAYCEAIVTSMPGAKLSFVGVGQARNQLLVI"
     gene            complement(11463..11942)
                     /locus_tag="VF272_02175"
     CDS             complement(11463..11942)
                     /locus_tag="VF272_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259715.1"
                     /translation="MQRISIEHPDPPTVATLEADWEGVCTLVSLLVTPPKSARPGLAY
                     IAEGTFNFHAVQAEGRPKHRINGDDGTWDGWAEDQTHSMKWVSLGSEAMLLAQRLDRG
                     WVLTYTGPTIGEDPEGNYLGFLIKVGSESGMIQNIRIKPVTDDARRKARVFGLAVVS"
     gene            12199..>13800
                     /gene="topA"
                     /locus_tag="VF272_02180"
     CDS             12199..>13800
                     /gene="topA"
                     /locus_tag="VF272_02180"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX7259716.1"
                     /translation="MTKHLVIVESPAKAKTIEQYLGNEYEVVASFGHVRDLPKKGMSI
                     DIEHGFTPTYEINADKKKTVAELRKKAKAAKSVWLASDEDREGEAIAWHLCIALGLKP
                     ETTKRIVFHEITKPAIQHAIANPRTVYQHLFEAQQARRVLDRLVGYELSPVLWKKIQP
                     GLSAGRVQSVAVRLIVDREREITSFKPAVLFRTYGYFDVKGHALKTELTKRIADESEV
                     QALLERLKNSAFEVENLEAKPGKRSPAAPFITSTLQQEASRKLGFSVRQTMSVAQRLY
                     EAGLITYMRTDSVNLSDTALVGAEKEIITSYGKPYAQRRTYTTKSAGAQEAHEAIRPT
                     DFARHSITGDAQQAKLYQLIWQRAVASQMADAKLEKTIVTVAPKDQPERFSAQAETIQ
                     FDGFLKVYTEGRDEEEDEESNLLPPMEIGQSLPLTKMIARQNFSRPKPRYTEASLVRQ
                     LEEMGIGRPSTFAPTISTIQDRQYVIKSDLEGIERQAIELTMVSRTIQRAELTETTGA
                     DRNKLMPTPVGELVTDFLVKFFPGIV"
BASE COUNT         3531 a         3576 c         3607 g         3086 t
ORIGIN      
        1 atcggccggc ctaatgttgg caaatcgagc ttgttcaacc ggctggtagg cgtgcgccaa
       61 gctataacgc atgaaaccgc cggaaccacc cgcgacgcca actatgggtc actgacctgg
      121 aatggcaagc atttcatctt ggctgatacc gccggtttgt acaaagccga caacgacacg
      181 cttgaaatgg cggttcagga gcagattacc gagattgcca acctggctga tgtcttggtc
      241 gtagtaatcg atgccggcgt aatgattacc aaggaagacc aactggccgc ccgtttggcc
      301 cgcaagaccg gcaaaccaat ggtgctggcg gttaataaga ttgatacggc ccaggccggt
      361 ccggtcgatc ctcccgagca gttgggcatc gacccgatcg ttcaaacctc ggcggtacat
      421 ggccgcggca ccggtgatct gcttgatgcg attgccgaac atctggctgc cgtaccggaa
      481 ccgccatttg aagcggacgt tgttactctt gccttactcg ggcgccctaa tgttggcaag
      541 tccagtttac tgaatgctat ggcggggaaa caacaagcga tagtatctga acgcgccggt
      601 accacccgcg atattggcag tattgatatt acgtaccacg gcaaaaccat tcgcattctc
      661 gatacggccg gtttacgccg ccgcggtaag attgaacagg ggatagagaa gtattccagc
      721 ctgcgaacct tggcagcgat tgcgcgagcc gatattgggg ttttggtgat ggacgcaacc
      781 gagccaagcg tggcgggaga ccaacacatt gccggagccg tggtagacgc cggtaaaggc
      841 ttaatcttgg tcacgaacaa gtgggatgcg gtccaagata aggatgataa aacccaggtt
      901 cggtttacgc gcaagctaat cagcgacttt gcctttgccc attgggctcc gctggtgtat
      961 acctcggcaa cccaaggctt aaacgttact caactgttta agcttaccca ggagattgcc
     1021 ctcatccgca ctaaaaccgt tcctaccggt gagcttaatc ggctgatgga gaaaatgata
     1081 gccaagcaag ccccagccgg ccttaaaaat cgccaaccta aaattaagta cgctacccaa
     1141 accggcacca aaccaccaac tttcacactg ttttcaagct atccggaaat ggttcacttt
     1201 tcgtatacgc gctatttgga aaatggtttg cgcgaggcat ttggcttcga cggtactccg
     1261 ataaaactgg aatttagagg gaaacaaaag tagtttttta acagggaagc atttcttttt
     1321 tgctctactc aaatctgagt acttacgtac ttactttatg cacagatatg agtacttttt
     1381 gagtacttgg gtactcaaaa gtgagtatct gaaaaagaag ttctggctag ccattgcttg
     1441 ggtggctcaa gatacgacaa ttaggtagtg aggtttactc attgctataa tcaatcttgt
     1501 tatgtcctta ctactaccat tcatatgaaa ctgatcattg gtctcggcaa tatcggcaag
     1561 gaataccacg caacgcgcca caacctcggg ttcggcgtag tagacatgct cgccattacc
     1621 ttcggcaaac aaccctccga cttcaccaag cacgctaaag ctgccgcaga aattcttgac
     1681 ttgaaaagcg agttcggctg catgctggtg cggcctacca cgatgatgaa cttgagcggc
     1741 caggccgtgc aagctctggc tcattattac aaaatagctc cgcataatat ctgggttatt
     1801 tatgatgatg tcgacctgcc gtttggacac atgcgagtaa ggctcggcgg cggcagcgcc
     1861 gggcataatg gcataaaatc aattatgcac catattggtg atgagttctg gcgggtgagg
     1921 ctgggaattg ctaatgatta tttagcgacc actcctaccg ataagtttgt actcgacccg
     1981 tttatggcag aggaagccac gaaggtgcca gcactgcttg aaaccgcggc taattatatt
     2041 gaagcagcgc tgctcagcgg aaacctatcc gaccatagcc aacacctgca ctaatttaat
     2101 catttggtaa taccggggtg gcatacttac tatacgtcca cacacaataa aaccccatga
     2161 tagggtggtg aagtccacta acacagggtt ttattgcacc ctccaatact tgctaaaatg
     2221 cagcattttg gcaatccggc tcacagcggg atcgagtatt cgagggactg aactacctgc
     2281 cggtacctaa aggagggtgc atttaagtta ccatgcaggc aggtcagtag ggtttactgg
     2341 tttaaggagc gaccttaaga aaggcatgaa taagagcata gcacacattg ttataaatgt
     2401 caaccatatt tatgcaaccc tctaagaaga ttgcacaagt aaactatgat aatgaccaat
     2461 acccggtcat ccttagagaa attggcagcc cgccacagcg gctgtatgtc cggggcgagc
     2521 tttcgcgagc tccgcatatt gcaattgtag gatcaaggcg cccgactgca tacggtgaac
     2581 aggtcaccta catgcttgcc agtgaattgg cagcagccgg catatgtata gtctcgggtt
     2641 tagccctggg catcgacggc atcgctcacc gagcggcttt agaggccggc ggtaccacaa
     2701 ttgcggtact tggttccggg ttaaatcaag tgtatccggc ccgcaaccgc gggatcgcag
     2761 cccagatcag cgctggaaga ggagcggtag ttagcgagta cgaagcagcg atgccggccc
     2821 tgccgcatca ttttcccctg cgtaatcgaa ttattgcggg acttagcctc ggtgttattg
     2881 tcactgaggc cgatgcctca agcggttcgt tgattacggc cgaccacgca ctgcaagcca
     2941 atcgaacggt tatggccgtt cccggcaaca ttaccagcct gcgctcagcc ggtccccaca
     3001 agctattgcg agacggggcg attccgatta ccagtgccgc cgaagtcctg aacgccctta
     3061 atctggatat tgcgagtctt tcgcaatcac ggccggcacc ggccagccag caagaataca
     3121 tcatcctttc tctcctaagc caaggggtgt caaacgggcg ggatttaatt gaacagagcg
     3181 gacttgagcc agcggttttt gcccggacta tcagtatcat ggagatcagc gggaaggtat
     3241 acaacctggg tattggtaac tgggtccggc gttaagtccg atggacaatc tgacgctcct
     3301 ggcgtaccaa gaaacgcaat gcaatgtaga gtagaatgct ggcaataaag ccggggaggg
     3361 cactgaattc cttctgataa aacgagaaga attgcactac gaaaatatcg attaagagtg
     3421 atttggtgaa cagttcatag gcccgcagcc gtgagctgcg aataattgca atgccggcga
     3481 cgacgaagcc ggtagaaatc aacgaggcgc caaactgaag cgttgacacc accgcgaacc
     3541 cttggagcgg aacctgagtg taccagcggg aagcatccac caccgcatcg gttaagaaaa
     3601 tgccggcctt aagcgcaaag accgtatcga tcaggcgaat cgcccaggtg gtggccatga
     3661 ttttctgata gcgagactca atctgacgca gccagcggcg tatgagggcc ggttcgctgt
     3721 ctttttgcgc accaatccgc tctaaggctt tttccaacgg ctttaccaaa gggtggttcg
     3781 ggtctgagcg gtgcaagttc gctaaaacgc gcttaagctc cggtcgatca agttcgttaa
     3841 ttacggcttc ttcaagcatg ttgagagcat tgagcaaatg ctcttcttta ctaaaccgcc
     3901 gttgtcgatt agaaagcgaa cgaaacatta aaaacatgat cacaaaaacc aagtaaatta
     3961 aggctaccgt aggctgaaaa aagtagttat tatcatgcgt tacatatttg cctaattcat
     4021 caataaatag tccgaatcca atgccgccga ttacgctggc tagccgctgc acccggttcc
     4081 ccagaaatgc cattgtaagc accaaggcgg caaccatcaa tacaccgccc ggcaacatat
     4141 gggccacatg cagttcgctg gtagacagct ggggataccc agtaagagtg agaagaaaac
     4201 gcaccagcag aacgctggaa attgccgcga ctaaaaacag ctctaagagc tcgccggatc
     4261 gatcattccg atagagataa cgcggcatca ttgtatcgaa attgtagcat aacaggtcta
     4321 atccattgac accgcgtgct ggttatggtt gaatcgtgat gtcccgttca caaccaattg
     4381 aggccagaat gaacgagtcc ttgcaaatcg ttccacgcat cactgtcgag gcaaaaccgg
     4441 gcgaccgcct ggtgttctgt tcgcggcagg atgacgtctg ggtcatcaag gccgacctga
     4501 atcccgtggg cgtcggcgac gatcctcgca tcatctgctg ggaacgcccc catgaggggg
     4561 tgtgctggtt ggtacacatc accggcggtc agctccgcct caaggagtac cggcctggca
     4621 tcaggccgct cgaatggttc attccgttca gcttccagcc gagtggactg cttctgcaga
     4681 tcatcgatgg ctgggtgccc aaagtgctcc ggggtaggat cgacgacgcg ctggccaagc
     4741 ccccggccac agctcagtag caccgcggac accttcaccc actcacaaaa gccctctgct
     4801 gcggcgttgc cctctgctga gggcttcgtc gcgccatacc gctaaatata aggtaaactt
     4861 aagagtacca atgaacgtat ggtcccagct tcaccaagca cataagccat tttttatttt
     4921 tgctcccatg gatgatgtca ccgacaccgt ctttcgcgaa gtggtggcac gcgccgccaa
     4981 accggatcta accatgaccg agttcgcttc aagtgatggt tttgcccatc cgaagggacg
     5041 cccaagcgtc gaacagcggc tgcgggttaa cgacaccgag gttgagctgg ccgtgccgtt
     5101 gattgctcaa atttggggca accatcccga tcattattat gcaatggcca aggacctagc
     5161 cgctcggcag caatttgccg gtatagatat caatatgggg tgtccggaaa aggggatcgt
     5221 aaaacgaggc tgctgcggtg gattgataaa agcagaaaat tgggagaacg ccgctgccat
     5281 cattgaggcc accaaagccg gtgccggaaa tcttccggtg agcgttaaaa cccggatcgg
     5341 tctcggaaca atcattaccg aagaatgggt ttcgcatttg cttaaacaag atattgcggc
     5401 actgattatt catggccgta ccgtgcgcga gatgagccgg gttccagccc attgggatga
     5461 aatcggcaag gcggtaaaac tgcgcgacca gctagcgccg aaaaccttga tgatcggaaa
     5521 cggcgacgta ctcagccgca atcagggcga acggttggca gctgcataca accttgatgg
     5581 cattatgatc ggccgcgggg tatttcaaga tccgtttgta tttgcctcca aggaagcaga
     5641 gcaccctccg gaggagatgt ttgcgatatt gctccaccat gtgaatcttt acgactcaac
     5701 ctggggcgac gctaaatcgt ataatccgtt aaaacggttt tttaaaatgt atgtaagtgg
     5761 ctttcccggt gcagccgaac tgcgtgcccg gctgatggat accaagacac ccgatgaagc
     5821 ccgcaagatt atgggtcgga taaccccatc tggcattgtt gcctaaacta ttgtattatc
     5881 tcttggttca cagcgcttcg ttcaggcagt gccccaccat caccgatcga aagagctagc
     5941 atgcccttta ttaagaggcg gcgcctgtcc agaaaggcga gacaacaggt tgcaaccgac
     6001 aaagcctgcc tcatcagata ccgactggag aaggcgccga ccgtgaaagg cgtcgacttc
     6061 atcgtcagac acaacgccag cgtcgtgttc ctgattgccg cagatccgca gcatcaagca
     6121 cagtgggcca aaagcctgct ctaccggacc tgccaacgcc agatgacagc agcgcgcaag
     6181 gaccgaatcg accgtgcact ggaagttctg gaggtcagcg gtacgggact ggaccccgag
     6241 caccatgacg tgttcctcat tgcgaggcgt tagaacgtgt gccggcacgc accacaagac
     6301 tcacgagaaa aagaccccgg ggtcaactcc tgtgggtctt tttctcttta ttgttggtac
     6361 ccgcaacgaa cccccgactg gcgggatccg ctggtcgggg gctggggccc tgaatgatgt
     6421 gggctagcgc ttccagggac ggtgcagcga gggccagaaa cgaccccatg agcggcgcaa
     6481 ccggtgcctc aatggcatca tcgagacgat gactcgatcc atctttttgg cggccgcatg
     6541 atcgagggca aagccaatgg tctttcgatt gtgaccacgc agatgcacaa cctcgccctt
     6601 actgctcgaa cggatgaccc agagcagctc ctcaacctct gccgctgaaa tgtcaccctc
     6661 ctcgggcaga agctcggaga tatgtacggc cagatgcaga gccacaccat catctgggtc
     6721 gtagatgaag ccgtcctgct gccgcagata gcgctgcagg cgttcgattt gcagccgctg
     6781 agctcgggtg ggcttcatgc gcggcggctc gctgagacga tgatgcagtg acatgatttc
     6841 aatccttcca atcgaaagcg ggtacattct actgaagcgt tatatagtta tagcatgaat
     6901 taactgttcc tacaagtttg cgcagcctgg ctggctacgc tacaattatg ctaacattta
     6961 aataactaat atgaagagtg cagcgaggga tctagcccga tgacctgccg gcaaccctgg
     7021 aaaaccagta ggtgctttca tctagcccgc aagggagaca tgtgctttta agctctttct
     7081 taccgaaccg ggggaaggaa attttttatg accagcaatg tatttacctc ggaatctgtg
     7141 tgtgccggcc atcccgataa ggtctgtgat cagattagcg atgccatttt agacgaagta
     7201 ttgcgccagg atccgaatgg ccgggtagcc gttgaaactg ccgttactgc caaccggatt
     7261 attttattgg gcgagcttac tactaccgcc aaaatcgatt ttgtcaaggt ttctcgagag
     7321 caggttaagc ggctggggta caccgatccc gactttcact tcacccccaa ctgcgatgta
     7381 gaagtttaca ttcgcagcca atcgccagaa attgctgcag gagtcgacct agacggagct
     7441 ggcgaccaag gtatgatgtt tggctatgcc tgtcatgaaa ccaagcagct catgccgctg
     7501 ccgattatga tggcccattc actaacacgg gcgattgatg cagcccgtga aagcggtacc
     7561 ctcccatatt tgcgccccga cggcaaagct caagtcacca ttcgttatga gaatgacatg
     7621 ccggttggaa ttgagcatat taccatggcg gttccgcata acgaagaaat cgagcgcaag
     7681 caagtccacg ccgacctggt aactgaagtt gttttgccgg tgcttgcaga gtatggactg
     7741 gatatgggca gcgccaaagt gatcgtaaac ggtaccggtg tttggcacgc acccgggcca
     7801 gccagcgacg ccggactgac cggacgcaag attattgtcg attcttatgg cggctatgcc
     7861 cgagtcggcg gcggcgcttt tagcggtaag gatccgacca aggttgatcg ttcgggtgcc
     7921 tacgcagccc gattcctggc taaaaatatt gtcgccagcg gcatggctga ccgctgcgag
     7981 gttggtttgg cctattttat tggtgctaag cagccggtaa tgcatcaaat taacaccttc
     8041 ggcactgcga aagaaagtca aaaagcaatc gccgcttttt ctaccgagat tcttgatacg
     8101 tcggtaaagg ggattattgc tgcgctcgac ctgcgccgcc cgatttactt agatacggcc
     8161 gcttacggcc actttggccg cgatgacgtg agctgggaaa aagtggtcga gcacgccaag
     8221 atatctgcca acgcctaggg ggcttctcgc gacagcaacc catagagttc attttggctt
     8281 atttttacta atgcacattt gtaacacttt gccacaaatg tgcattagct gaaaacgcct
     8341 gacacagaag ggtagtcggc gagctgccag accagaatgg tacactagga ctatatgaag
     8401 tggctgacaa agtcgaatta tctgcacttt cttattcatc ccgcctattt ttggctggag
     8461 aagcacgata aagaaaaact gccgccattt gatgacgctg cccaggcgat catggacgcc
     8521 gggaatgaaa ttgaagaata cgcccgcgaa cgatttcccc taggacagct agtaccgggc
     8581 gggctgttcg accagattga cgaaagcacc aagttgctca agcacgctgc tgcaggagaa
     8641 accttatttc aggcagcggt gttgaccgat caaaaatggt acgcgcgctg tgatgttgtt
     8701 ataaaaaata atggcggatc atgggacata tatgagatta aaagcgctac aaaagtaaaa
     8761 ccggaacatc ttcatgatgt agcatttcag aagattgcac tagaagaatg tgggcatcct
     8821 gtgagaaata cctacattgt gcatatttcc agtcactatg ttcgccaggg cgcagttgat
     8881 tcccagaaat tatttgagca agttgatgta accgcggccg tcgatggatt gctccataaa
     8941 accaagcgcg atattgccgc cgcacaagca atgatggctt tgccggaatg cccaagcgat
     9001 tcgccggcct tagcaagtaa ctggtatgcc tggcgcgaca tctatcgcca tctgcatcct
     9061 gaaattaccg atcttagcat ctataattta gcgagactta agccggagca gctaagcgct
     9121 cttgcaaaac tcggtataag cgacatgcta agcattcccg attcctttgc attggggtcg
     9181 cagcaaaaag cccaacttca agcgctcagg gccggggagc cgattattca tccggtcaaa
     9241 atagcagcgg cgctggcggg gctcgcctat ccattgtatt tcttcgatta tgagacggtt
     9301 ggcccgggta tcccggcata tgatgggacc aagccctatc agcaggtgcc ctttcagtat
     9361 tcgctgcaca tacttgataa accgggaggt aagctgcgcc acaccgagtt tttagcaacc
     9421 ggcaacgcta atcccatgcc ggcgctgctt gagcaaatgc agcatgatat tggacccatc
     9481 ggcagcgtta ttgtatggta caagggcttc gagatgggtg ttaatgatgc catggcggcc
     9541 atttatccgg agcatgcggc ctttttacac caggtgaaca cccgtgtctt cgaccttatg
     9601 gaaattttca gtaattttca ctacgtcgat gctgcattcg gaggcagcgc atcgattaag
     9661 aaggttcttc cggtagtcgt gccagagctt acttacagcg atctagcaat ccagaagggg
     9721 gatgttgcct caaagctttg ggagcaaacc gcccagggga agattaccgg aaaacaggct
     9781 gagaaaatct atgccgattt gcggatctac tgcggccagg ataccttagc gatggtgcgg
     9841 atttacgagt ttttgatgcg tattacgaaa acagctccgg gcgcgcaact tagcctacta
     9901 ggctaagtcg gcttttctgc tataattcca gcgtgaaaca gccacaggtt agcgccgtca
     9961 tcggcggaca atggggagac gaaggcaagg gcaagattgt tgattacttg gctgccgatg
    10021 ccgacgtagt tatccgctcg caaggagggg acaatgccgg acataccgtt ttaaatgaac
    10081 gcggtaagtt tggcttgcat ttagtgccgg ccggaatctt taatcccgac accattaaca
    10141 ttatgggcgc cggtgtggct cttaatccca aaaccttact taaagaaatg aaagagctgg
    10201 gcgaacgagg agtcagtttt gataatttaa ttattagtcc gaaagctcat ctggccttcg
    10261 attatcatct ttttattgat cagagccaag agcatgcccg tggtaaaaat cgaattgaaa
    10321 ccaccaagcg cggcattggc cctacttata tggataaagc cgaacgcatc ggcctgcagg
    10381 ccaagttact actagaaccc gatcgctgca tggaagaact ggcccgtgtg ttggcactca
    10441 agcgcaaaca tctcgaccaa gacaatgagg ttctctatcc ggaatattat gaggactttg
    10501 taaaagaggc gggtgcggcg ctaaaagatt tcattcgtcc aaccgaacgc attatcgaag
    10561 agcatttgca caaaggcagc cggtttgtac tggagggagc ccatggcaca ctgcttgacc
    10621 tcgaccacgg cacctaccca atggtaaccg cctcaaactc caccgtggga ggattgttgg
    10681 tgggagccgg catgccgccg ctagcgctta ataaggtggt aggcatcttt aagtcatatc
    10741 aaacccgcgt tggggcagga ggaatgccta cggagcttga taatgaacta ggcgagcaca
    10801 tccgcacacg gggacatgaa ttcggcacca ccaccggccg tccgcgccga gttggtttct
    10861 ttgatggcgt agccgccttg tatgcccacc gaattaatgg ctttaccgac atcgccgtca
    10921 ccaggatcga caccttggcc gacgtgaagg agcttaagat tgctaccggc tacaaggata
    10981 ccgctggctg gccaactgat ttcccaaccg ataacacccg cctaagcgac tgcatacccg
    11041 cctacgagtc ttacgaaggc tggaccgaag ctgaaatcgc caaagcctca acgtttgatg
    11101 aactgcccct aagtgctaaa gcctattgtg aagcgattgt tactagcatg ccgggcgcga
    11161 agctcagctt cgtaggtgta gggcaagccc gcaaccagtt actcgtcatt tagccttgtt
    11221 caaccgcacc gaggatcgtg ctacatactt ctattcaagc gtttagtatc tcaaggatac
    11281 tactattcaa ttgcatttat atgaagtcgc gccagcaata gtatcctacg gatactaaag
    11341 gtaaagaaaa acggtgatgt gcaccgcgta gccatgatca gcctaaattt ttaagctact
    11401 aacgttaaaa actggggctg aagccggaac gagggagaac cctcgctccg gcgcggcaca
    11461 tcttagctga cgacggccaa gccgaagact ctcgccttgc ggcgtgcatc gtcggtcacg
    11521 ggcttgatcc tgatgttctg aatcatcccg gactcagatc cgaccttgat gagaaaaccc
    11581 aggtaattgc cctcggggtc ctcgccgatg gtcgggccgg tgtaggtgag cacccagccg
    11641 cgatcaagcc tctgtgccag aagcatcgct tcggatccaa gcgaaaccca cttcatggaa
    11701 tgagtctgat cctcagccca gccgtcccat gtaccgtcat caccgttgat ccgatgcttc
    11761 ggcctgcctt ccgcctgtac cgcgtggaag ttgaacgtgc cctccgcgat ataggcaagg
    11821 ccggggcggg cggacttggg cggtgttacc aggagggata caagcgtgca caccccctcc
    11881 cagtccgcct ccaacgtcgc gacggttggc ggatcggggt gttcgatact aatacgctgc
    11941 ataacgatct ccgtgcgttt taccccgccg ggattgagct cgaagccagt ctccgaacga
    12001 cgtatacatg catataaatg cacgcataaa ccgttcggag atactgccag ttgagacata
    12061 ctgttaaagg tactcgctcg aaaaagtctc gattgaacag atattatcgt gtcatgttta
    12121 taatgattcg tcaagtttga ggagtggctc cgcgaaaagc ctttacacgc ctcatacata
    12181 tatatacagt agccgagtat gaccaaacac ttagttattg tagaatcccc agcgaaagcc
    12241 aaaaccattg aacagtacct cggaaacgag tacgaagtgg ttgcgagttt tggtcatgtc
    12301 cgcgacttgc ctaaaaaggg gatgtctatc gatatcgagc atggcttcac gccaacctat
    12361 gaaataaatg ccgataagaa aaagaccgtc gccgagctgc gtaaaaaagc caaggctgca
    12421 aaaagcgttt ggctggcaag cgatgaagac cgcgagggag aggcgattgc ctggcatttg
    12481 tgcatcgctt tgggccttaa accggaaacc accaagcgga ttgttttcca tgaaattacc
    12541 aaaccggcga ttcagcatgc tattgctaat ccgcgaaccg tatatcagca tttatttgaa
    12601 gcccaacagg ccaggcgtgt tcttgaccgg ctggtagggt atgaactttc gccagtatta
    12661 tggaaaaaaa tccaaccggg cttaagtgcc ggacgagtgc agtcagttgc ggtgcggctg
    12721 attgtcgacc gagagcggga gattaccagc tttaaacctg ccgtcctgtt tcgtacatac
    12781 ggttattttg acgtaaaagg acatgcactc aaaaccgagt taaccaaacg gattgcggat
    12841 gaaagtgaag tgcaggcctt gcttgagcgg ctgaaaaatt ccgccttcga agttgaaaat
    12901 ttggaagcca agcccgggaa acgcagcccg gcggccccat ttattacctc aaccctacaa
    12961 caagaagcta gtcgcaaact gggcttttcg gtgcgacaaa ccatgtcggt cgcccaacgg
    13021 ctctatgagg ccggactcat cacctatatg cgtaccgact ccgtcaattt atccgatacg
    13081 gcattggttg gcgccgaaaa ggaaatcatt accagctacg gcaaacccta tgcccagcgc
    13141 cgaacctaca ccaccaaatc ggctggagcg caggaggccc acgaagctat tcgtcccacc
    13201 gactttgccc gccatagtat taccggcgat gcccagcagg ctaaactcta tcaacttatc
    13261 tggcagcggg cagtggcctc gcaaatggcc gatgcaaaac tggaaaaaac catcgtcacg
    13321 gtagcgccga aggatcagcc agagcggttt agcgcccagg ccgaaaccat tcagttcgat
    13381 gggtttctta aagtctatac cgaaggccgc gatgaagaag aggacgaaga gagcaattta
    13441 ctgcctccga tggagattgg ccagagcctg ccgctgacaa aaatgatagc ccggcaaaac
    13501 tttagccggc caaagccgcg ctacactgaa gcaagtttgg tacgccaact ggaagaaatg
    13561 ggcattggac ggccatcaac cttcgcgcca acgatttcta ccatccagga tcgccagtat
    13621 gttattaaaa gtgatctcga aggcatcgag cgccaggcaa tagaactcac gatggtgtcg
    13681 cgaaccattc agcgtgccga gcttaccgaa accaccggcg ccgaccgcaa caaacttatg
    13741 ccaacgccag taggggagtt ggtgaccgat ttcctggtta aatttttccc cggcatcgtc
//
LOCUS       DASVGB010000073         7413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_688969, whole genome shotgun sequence.
ACCESSION   DASVGB010000073 DASVGB010000000
VERSION     DASVGB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688969"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF272_02185"
     CDS             complement(<1..322)
                     /locus_tag="VF272_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX7259717.1"
                     /translation="MSNQRLHQIRQQLATHRVDALLITHLPMIRWAVGFSGSSGLLYI
                     DANQVLFATDTRYQTQAAEEATTVDTILMAIQGQLAPPLAEQTPNTVARIGFSSEHLT
                     VTELA"
     gene            640..1008
                     /locus_tag="VF272_02190"
     CDS             640..1008
                     /locus_tag="VF272_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259718.1"
                     /translation="MNNFERKLDDKKRLTIPVEVRPEFEGGYVVLTKGYDNTLALYSQ
                     KYWEEVIDPALEGDFRDVESFRLNRQIQSGQSRTHMDQKQGRITIEQRFLDYAGITKT
                     INAFRVKTAAGRSYWAIEAA"
     gene            1359..2282
                     /gene="rsmH"
                     /locus_tag="VF272_02195"
     CDS             1359..2282
                     /gene="rsmH"
                     /locus_tag="VF272_02195"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7259719.1"
                     /translation="MKQSSHIPVLINDVLRLLNPQPGETYFDGTAGLGGHAAEVLEQM
                     GPSGQAILADRDVQAITQLATRFEPLGPRVAIWHTDYLAAAGQLIESNRAVDMILLDL
                     GVSSSQLDRAERGFSFTHDGPLDMRMDQTLLRSAADIVNTVSEKELADLIYEYGEERK
                     SRRIAKAIVRERPIHTTSALAAVVRSAAGPSKDTIHPATRTFQALRIVVNEELYQLEQ
                     ALPLLTALLNPGGRIVVISFHSLEDRLVKVYFRRESRDCICPPRQPICTCGHTASLKL
                     LTNRPLTGITDAFNPRARSAKLRAAVKLTQK"
     gene            2506..2805
                     /locus_tag="VF272_02200"
     CDS             2506..2805
                     /locus_tag="VF272_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259720.1"
                     /translation="MRRNQNLYRQKAELKLGPVSSGFVLIAITAVLALLYLNAVTKTA
                     VFGYELSQMQQARNEAVAANQELEIEAARLTSIQQIQKSQAVAKLVPTTQVTYVK"
     gene            2808..4556
                     /locus_tag="VF272_02205"
     CDS             2808..4556
                     /locus_tag="VF272_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7259721.1"
                     /translation="MSPPSLAANASNQRLDWLYIAIVLLGILTVGRLFYIQIIQHGDF
                     ARLAANEHQRKYEVPADRGQIYLLDGDTKVPLALNQQLKLLYADPSIVTNKRRTAEQL
                     AKITGQPAEKYVNAMEKGKEYALLETKINAKTADKIKALRLRGIGFKDQTFRTYPEGS
                     LASQVLGFVNADGKGQYGVEGFLNKELGGQPGHLNAKTDTNGVPIASSDNLDRAPVRG
                     TNVVLTIDRSVQAQAEKFLAEGVINVKAESGSVIVMDPNTGAVKAMANYPTFDPNDYS
                     KVTDYRVFSNAIVSDNFEPGSVFKLFTVAAGLDSHKITPNSTYNDTGCEKIDNYNVCN
                     AENHKLGPNTTMTVVLRDSLNTGVMYILKLMGSDPATITKASKQALHRYITQRFRFGQ
                     RTGIEQAGEAAGFVNPTTTSNVTYANMSFGQGISVTTLQTATAISAIANGGKLYKPYL
                     VEKYITADGSEVAARPKVVNPHVISTQAAKDLGIMMEVVVHRGSGYRAYTPGYKIAGK
                     TGTAQIANPNGPGYLPDKNIGTFAGFAPVEDPKFVVMVRINKPNTPGFAEVTTVPVFA
                     NLTRWLLQYYAVPPSG"
     gene            4710..5747
                     /gene="mraY"
                     /locus_tag="VF272_02210"
     CDS             4710..5747
                     /gene="mraY"
                     /locus_tag="VF272_02210"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7259722.1"
                     /translation="MTFVYEADIVSLSRILALGFVGFALSMVLTPVYTSLAYRYKWWK
                     QVRDTAVTGEKAPIFYKLHAAKHKRNIPTMGGILIVVSIVLVTLLLNLSRNQTYLPLA
                     ILVSAGLVGLLDDYINIRGNGNGTAGLHSRLKFLLITVIAVLGALYFYFKLGYHLIHI
                     PAVGDFSIGWLYIPLFIAVVVSTANAVNISDGLDGLSGGLLSTAFAAYGVIAYFQGSF
                     GIAGFCATIVGVMLTYTWFNIYPARFFMGDSGSFALGTTLGAIAVLTNAVVVLPIIAG
                     VFVLEAGSSALQIASKKLFKRKIFLSAPIHHHFEAIGWPETKVTMRLWVIAQVLGVVG
                     LTIGLLGNRIL"
     gene            5744..6955
                     /gene="ftsW"
                     /locus_tag="VF272_02215"
     CDS             5744..6955
                     /gene="ftsW"
                     /locus_tag="VF272_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX7259723.1"
                     /translation="MSPRGGQLSVRGHRGDYVLGLAVFMLLAFGLIMMYNINPALSQK
                     QGAETSYFFNQLTFVGIGVGAWAVISSLYYKVWRRLALPLLILAIAAVVAVLTPLGYS
                     ALGASRWLALGPFSFQPSELLKLALVIYLAVWFERHYKVLGSFKQGVVPYIVMLAIAG
                     LFVAILQRDLGTMMVIALATLGMFYVAGIRLKHLAILAASGTVVAALAIMAFPHRISR
                     FMTFLDPMSDCSGRGYHICQSLIGIGSGGLFGVGLGQSVQIYGYLPEAPNDSIFAVIG
                     EEFGLIGSLIVLTVYAVLIYRGLEIARRAPDMFARLLAAGITLIFLFQTFINIAAMLS
                     IVPLTGIPLPFVSYGGTSLVIMMVAAGLLFNISKYTLRKDSHADNRQRRGNQRPRFAG
                     TSNRRRVTLGQ"
     gene            6870..>7413
                     /locus_tag="VF272_02220"
     CDS             6870..>7413
                     /locus_tag="VF272_02220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7259724.1"
                     /translation="MRIIVSGGGTSGHVSPVLATVDALRSGSNPPEILFVGSHGGVEA
                     GLSRAAGLPFRGILGGKFRRLPGAGIAGNLRQWRHIIWNLRDLLYMLVGTLQALWIIG
                     WYRPDVIFNKSGPPGLPVGAAAWFLGIPMVLHEPDSTPGLGTRVMGRWATVIATGFPI
                     QLYPLYLQKKLVHTGTPVQPV"
BASE COUNT         1836 a         1774 c         1901 g         1902 t
ORIGIN      
        1 ttgctagctc cgtcaccgtc aggtgctcgc tgctaaagcc aattcgcgca acagtgtttg
       61 gtgtttgctc ggctaacggg ggagccagtt ggccctgaat cgccatcaga attgtatcga
      121 cagttgttgc ctcctcggct gcctgcgttt ggtagcgggt gtcggtcgca aacaggactt
      181 gattggcatc tatatataag agtccgctgc taccggaaaa accaaccgcc cagcgaatca
      241 ttggaagatg ggtgataagt agggcatcaa ccctgtgcgt ggcaagttgt tggcggatct
      301 gatggagacg ctggttggac atgcagtcat tgtagcaaat ccaacccgct tattttttaa
      361 aaccagcgta ctcaaatgtg agtatccatg tactcaagaa gtactcacat ttgtgtagta
      421 attgagcatc atagtactca gatatgagta cgtgcataaa aatgatattt ttgtctccaa
      481 aagaaaggct taggtgagcc tttcttacgc ttatgcttag aaataatgcc actatcgaca
      541 agaaagtggg taaaaggggg ttgacgtggg taaaagtggg tagtattctt aagtcattga
      601 gttgaacaac gactcaaaga gggttgaaac ggagcacacg tgaacaattt cgaacgcaaa
      661 ctggatgata aaaaaagact gaccattccg gtcgaagtcc ggcccgagtt tgaaggcggt
      721 tacgttgtac tcactaaagg atacgacaac actctggccc tgtactcaca aaagtattgg
      781 gaggaggtga ttgatccggc gctggaaggg gattttcgcg atgtcgaaag tttccggctc
      841 aaccgccaaa tacagtccgg ccaaagtcgg acccatatgg atcaaaaaca aggtcgcatc
      901 accatagaac agcggttctt ggactacgcc ggcattacca aaaccattaa cgccttccgt
      961 gttaagaccg ccgccggccg ctcttactgg gcaatcgaag ccgcataacc cgtcaacctc
     1021 gttagtcaca cttgttcatt aaaaaccaaa acaaaaacat tcgaagttaa ctacttggca
     1081 gccagcacaa cggtctttag ggatccgtaa aaccctggct cctacctaaa aaaactccac
     1141 ctcacacaca cttttaagga attgtactac attcttttcg gaattatgac agttccttac
     1201 cacactcgta acgctatctc tagcgttaca cactcaaaaa aacaaaaacc gcaaacaaaa
     1261 agcgggctgc taagtaggtg acctcgaagc tctagtgccg ctgaggtcac agcaagtgca
     1321 tggtataatc atgcctttac atacagagtt accgcatgat gaaacaatcc tcccacattc
     1381 cggtgctgat taatgatgtt cttcggctgc ttaatccgca gccgggagaa acgtactttg
     1441 atggcacagc cggtttgggc ggccatgccg ctgaggtcct tgaacagatg gggccaagcg
     1501 gacaggcaat ccttgccgac agggatgtgc aagcgattac acaactggca acccgttttg
     1561 agcctctagg gccgcgggta gcgatttggc ataccgacta tctggccgca gccggccaat
     1621 taatcgagtc aaaccgggcg gttgatatga tcttgcttga tctgggtgtt tcgtcgtcgc
     1681 agctcgatcg agccgaacgc ggttttagct ttacgcatga cggtccgctt gatatgcgca
     1741 tggatcaaac gcttttacgc agcgccgccg atattgttaa tactgtatcg gaaaaggagt
     1801 tggctgattt gatttatgag tatggcgaag aacgtaagtc acggcgtatt gcaaaagcca
     1861 ttgtacgtga gcgcccaatc catactactt ccgccctggc agccgtggta cgcagcgcgg
     1921 cgggtccaag caaggacacc attcatcctg ctacccgcac ctttcaggca ttgcgaattg
     1981 tagtgaatga agagctttac cagcttgagc aagctctgcc gcttttaacg gctttgctta
     2041 atccaggcgg acgtattgta gtgataagct ttcattcgct tgaagaccgt cttgtgaaag
     2101 tctactttag acgtgaatcc cgcgactgca tttgtccgcc ccggcaaccg atttgcacct
     2161 gcggtcatac tgccagcctg aaactgctca caaaccgacc gcttaccggc atcaccgacg
     2221 cttttaaccc gcgagcacga agcgcaaaac tccgtgccgc agtgaaacta acacaaaaat
     2281 aaggaggcct aatgacgtta gtcatcaaaa accagtcccc acgcgctaag cgcatccaag
     2341 tccgcatcgt taaataacga ttgcgtgact gccgcggttc gcggtgcaag attagccatc
     2401 ttcgagatga gcgccgcgaa ccgcccataa aaatctacca aaattcaaaa aaaacaaaaa
     2461 ccaacatcag gagtatttcc ctttatgatc actgctacta tggcaatgcg gcgaaaccag
     2521 aacctttacc gccaaaaagc cgagttaaaa ctaggaccgg tatcctccgg attcgtatta
     2581 attgcgataa ccgcagtcct ggcccttttg taccttaacg cagttaccaa aacagctgtt
     2641 ttcggttacg agctttcgca gatgcagcaa gcccgcaatg aagctgtcgc tgccaatcaa
     2701 gagttggaaa ttgaggctgc ccgtctaacg tcaattcaac agattcagaa atcacaagcc
     2761 gtggctaaat tagtgccaac cacccaagta acctacgtta aataaccatg tcgcctccaa
     2821 gcctagcagc caacgccagc aatcagcgcc tggactggct ctatattgca attgtgcttc
     2881 taggcatact caccgtaggc cgtctttttt atattcaaat tatccagcat ggcgattttg
     2941 ctcgtctggc tgccaacgaa caccagcgta aatatgaggt cccagctgac cgaggtcaaa
     3001 tttatttgtt agacggagac accaaagtcc ccttagcgct caatcaacaa ctcaagctct
     3061 tatatgctga tcccagtatt gttaccaata agcgtcgcac cgccgaacag ctggctaaga
     3121 ttaccggaca accggcggaa aaatatgtta acgccatgga gaaggggaag gaatatgcct
     3181 tgcttgaaac caaaataaac gctaaaaccg ccgacaaaat taaagctctt cgactgcgag
     3241 ggataggatt caaggatcaa acatttcgta cctatccgga gggcagtttg gcgtctcagg
     3301 tgctgggttt tgtgaatgcg gatggaaaag gacagtatgg ggtagaaggc ttcttgaata
     3361 aggagctggg cggccagcct gggcatctta atgctaagac tgataccaat ggagtgccga
     3421 ttgccagtag cgacaacctt gaccgagccc ccgttagggg taccaatgtg gtattaacga
     3481 ttgatcgaag cgtccaagct caagccgaaa agtttttggc cgaaggggtt atcaatgtaa
     3541 aggcggagtc gggaagcgtg attgttatgg atccgaacac cggtgctgta aaggccatgg
     3601 ccaattatcc tacctttgac ccgaacgact attcaaaagt aactgattac cgagtcttca
     3661 gtaacgccat agttagcgac aattttgagc cgggttcggt gtttaaactt tttactgtgg
     3721 ctgccggact tgatagtcat aaaatcacgc caaatagtac gtataacgat accggctgtg
     3781 aaaagattga taattataac gtctgcaatg ctgaaaatca taaactcggc cccaatacga
     3841 cgatgaccgt ggtgctgaga gattcgttaa ataccggtgt gatgtatatc ttgaagctca
     3901 tgggtagcga tcccgccacc attaccaaag catcgaaaca ggcgcttcac cgctatatta
     3961 cccagcgttt tcggtttggc caacgcactg gtattgagca ggccggcgag gcagccgggt
     4021 tcgtgaaccc cactaccacg agcaatgtta cctatgccaa tatgagcttc ggacagggaa
     4081 tatcggttac cacgctgcaa accgccacag caattagtgc aattgccaat ggtggaaagc
     4141 tgtataaacc gtatttggtg gaaaaataca ttacagcaga tggtagcgag gttgccgccc
     4201 gcccaaaagt ggtgaatcca catgttattt ctacgcaagc ggctaaagat ctaggaatca
     4261 tgatggaggt cgtggtgcac cgcgggtccg gttatcgcgc ttacacgccg gggtataaga
     4321 ttgccggtaa aaccggaacc gcccaaatcg ccaacccgaa tggtcccggc tatctgcccg
     4381 ataaaaatat tggtacgttt gccggtttcg cgccagtgga agatccgaag tttgtggtaa
     4441 tggtacgcat taataaacca aatacgccag gtttcgccga agttacgacc gttccggtct
     4501 ttgccaatct cacccgctgg ttgctccagt attacgcggt gccgccaagt ggataacatg
     4561 atatctgcat tcagccggag atgtacccgt ttttgcccag gcaccagcaa ccggcacagc
     4621 aatgtgcggc taaggcaggg ggaacatgcg cgcatctcag atatgaaaac gcttacgctg
     4681 atctgtaaaa catggtaaaa tgagccacaa tgacgtttgt atacgaagct gatattgttt
     4741 ctctctcacg aatcctggca ctcgggtttg ttgggtttgc gctttcgatg gttttaaccc
     4801 cggtttacac cagtttggcg tatcgttata agtggtggaa gcaggtacgt gatacggcag
     4861 taaccggcga aaaagcacca attttctata aactgcatgc ggcgaagcat aaacgtaata
     4921 ttccaaccat gggcggaata ttgattgttg tcagtattgt gctggtaaca ttgctcctaa
     4981 atctcagccg caaccaaacc tatttgccat tagcgatctt agtgtcggct ggactggtgg
     5041 ggttgcttga tgattacatt aatattcgtg gcaatggaaa cggtactgcc ggactacaca
     5101 gtcgccttaa atttttactt attacggtaa ttgccgtact tggcgcgctt tacttctact
     5161 ttaagctggg ttaccactta attcacatac cggcggtggg ggacttttcg atcggatggc
     5221 tgtatatccc gctgtttatt gcggtcgtag tatcaaccgc taatgccgtg aacattagcg
     5281 acggactcga tggtctgtcc ggcggactgc tctcaacggc atttgccgcc tacggcgtga
     5341 ttgcctactt ccaaggcagt tttgggattg ccggtttctg cgcaacaatt gtcggtgtta
     5401 tgctgaccta cacctggttt aatatttatc cggctcgctt tttcatggga gactcaggat
     5461 cgtttgcatt ggggacaaca ctaggtgcga tagccgtgct gaccaatgcc gtggtggtgt
     5521 tgccgattat tgccggggtt tttgtgcttg aggctggatc gagcgctttg cagatcgctt
     5581 caaaaaagct atttaaacga aagattttcc tttcggctcc gattcaccat cattttgaag
     5641 ccattggatg gccggaaact aaggttacta tgcggctctg ggttattgcg caggtcttgg
     5701 gtgtggtcgg tctaacgatt gggctgctag gaaatcgtat cttatgagtc cgcgtggcgg
     5761 ccagctgtcg gtgcgggggc atcgcggcga ctatgtactg gggctggcgg tctttatgct
     5821 tttggccttc gggctcatta tgatgtacaa catcaatccg gccttaagtc agaaacaagg
     5881 ggctgaaacc agttatttct ttaatcagct cacctttgtt ggtattgggg tgggagcatg
     5941 ggcagtgata agcagcctct attataaggt ttggcggcgt ttggcgctgc cattattgat
     6001 actggcaata gctgcggttg ttgccgtctt aacgccgctt gggtattcgg cattaggtgc
     6061 ctcaagatgg ttggcgctgg gaccgttttc ctttcagcct tccgaattgt tgaaactggc
     6121 tctggtgata tacctggcgg tttggtttga acggcactac aaggtattgg gctcctttaa
     6181 gcagggggtt gtgccgtata tcgttatgct ggcgatagcc ggattgtttg tagctatctt
     6241 gcagcgggat ttaggtacga tgatggttat tgccctagca acgcttggca tgttttatgt
     6301 tgccggaatt cgtcttaagc atctagctat tttagcagca tcaggtactg tagtagcggc
     6361 actagcaatt atggctttcc cgcatcgtat tagccggttt atgacctttc tggatccgat
     6421 gagcgactgc agcggccgtg gctatcatat ctgccagtcg ttgattggca ttggcagcgg
     6481 cggtctcttt ggggtggggt taggccagag cgttcaaata tacgggtatc ttcccgaggc
     6541 cccgaacgac tcaatttttg cggtaattgg tgaggagttt ggtctgattg gcagtttgat
     6601 tgtactgacc gtctatgcag tactgattta tcgcggtctg gagattgccc ggcgggctcc
     6661 cgatatgttt gcgcgcttgt tagcagcggg catcaccttg atttttctat ttcagacctt
     6721 catcaatatt gcggctatgc tttcaattgt accgttgacc ggtattccgc tgccgtttgt
     6781 aagctatggg ggtaccagcc tggtcattat gatggtggct gccgggttat tgtttaatat
     6841 ttctaagtac accttaagga aggatagcca tgcggataat cgtcagcggc gggggaacca
     6901 gcggccacgt ttcgccggta ctagcaaccg tcgacgcgtt acgctcgggc agtaacccgc
     6961 cagagatttt atttgtcggg tctcacggtg gcgttgaagc tggcctgtca cgggctgccg
     7021 gactaccatt tagaggaata ttgggcggaa agttccgtcg actgccagga gccggtattg
     7081 ccggcaattt gcgccaatgg cggcacatca tatggaatct gcgtgatctg ttatacatgc
     7141 tggtcggtac cttgcaggca ttgtggatta ttggctggta tcgtcccgat gtcatcttca
     7201 ataaaagtgg tcctcccgga ctgccggtgg gggcagctgc ctggtttcta ggtattccca
     7261 tggtactgca tgagccggat agcacccccg gacttggcac tagggtaatg gggcggtggg
     7321 ctaccgttat tgctaccggc tttccaatac agctttaccc gctatacctt caaaagaagc
     7381 tggtgcatac cggcacgccg gtccagccgg tca
//
LOCUS       DASVGB010000074         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_617912, whole genome shotgun sequence.
ACCESSION   DASVGB010000074 DASVGB010000000
VERSION     DASVGB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617912"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /gene="carB"
                     /locus_tag="VF272_02225"
     CDS             complement(<1..1820)
                     /gene="carB"
                     /locus_tag="VF272_02225"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016210187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7259725.1"
                     /translation="MKQPATPKKAVIFGSGGLRIGQAGEFDYSGSQAIKALKEEGITT
                     VLINPNIATVQTDSDMADRVYFESLDAATVTTILEQEKPDIVLLGFGGQTALNLGLEL
                     YDKGVFKRLNIRISGTSLETIKTTEDRQLFKASMEEIGTKTARSAVAYNLDEALAAAQ
                     SIGYPVMLRAGYSLGGLGSGRVDNDREMKRRASEALQAVPQLLIEEFLAGWKEVEYEV
                     VRDSKGNTITVCNMENLDPMGIHTGESIVVAPSQTLTNEEYHRLREVAIRTINHLKII
                     GECNIQYALNPQNGDYRVIEVNARLSRSSALASKATGYPLAYVAAKLVLGKHLHELTN
                     AVTGTTSAFFEPALDYVAVKMPRWDLAKLKSSDHTIGSEMKSVGEVMALGRSFPEALQ
                     KAIRMLNIGAHGLTQHPYTFKNPHDEISPATDRRIFAIHEAFTKGDSVAAIQKLSSID
                     RWFLEHLHDIGEIERRIRRRSLTKELLLEAKKSGFSDRVISTLTKTSEAAMRAQRIAW
                     GIQPVIKQIDTLAGEFEASTNYLYTTYHGDTNDVLPLGNRAVTVLGSGPYCIGSSVEF
                     DWCAVNTAKTLQKHGWQTVMVNSNPETVSTDFDLSERLYF"
     gene            complement(1817..3001)
                     /gene="carA"
                     /locus_tag="VF272_02230"
     CDS             complement(1817..3001)
                     /gene="carA"
                     /locus_tag="VF272_02230"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018298775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7259726.1"
                     /translation="MSAKRRPDRYHLRLTYLFGTFKRMHMDQELQLKNSLQQHGSRSE
                     RGVFFVNRPMQLHLSDGTVFQGLAPQWQAGQYTGEVVFNTGMTGYVESLTDPSYANQI
                     LVFTYPMIGNYGVQPDDAESDKIQASGVILSQVALKGSHSASQGSFLEWLHSQNIPVL
                     TSVDTRALTKHLREAGTMAGIISAETVDAGKVRLEPKFVSVNEPEVHKPKGTATKTVV
                     LVDCGAKNNILQSLLALGLQVRRVPYNYDYTAEDYDGVVLSNGPGDPTDYAATIAITR
                     KALEQDKPVFGICLGTQVMALAAGATTYKLKFGHRGHNQPCIEAATKRCYITSQNHGY
                     AIDETSLPKGWEVWFRNLNDRSVEGIRHSSKPFFSVQFHPEACPGPTDTAWLFKRFFE
                     SL"
BASE COUNT          723 a          837 c          799 g          747 t
ORIGIN      
        1 tcaaagtaga ggcgctcgga aaggtcaaaa tcggtagaga ccgtttcggg gtttgaattg
       61 accatcacgg tttgccaccc atgcttttgc agggttttgg cggtattaac ggcgcaccag
      121 tcaaactcaa ccgacgagcc aatgcagtac ggccccgagc caagcaccgt aaccgcgcgg
      181 ttgcccaacg gcagcacatc attggtgtcg ccatggtagg tggtgtagag atagttggtg
      241 gaggcctcaa attcgccggc cagcgtatca atctgtttaa taaccggttg aataccccag
      301 gcaatacgtt gagcacgcat agcggcttcg ctggttttgg taagcgtgct aataacgcga
      361 tcggaaaagc cgctcttttt ggcttcaagt agcagttctt tggtaagaga ccggcgccgg
      421 atgcgccgct caatctcccc aatgtcatgg aggtgctcca gaaaccaacg atcaatcgat
      481 gacagttttt gaatggccgc cacgctatcc cctttggtaa aagcctcgtg tatggcaaaa
      541 atccggcgat cggttgcagg gctgatttca tcgtgaggat ttttaaacgt ataggggtgc
      601 tgcgttaatc catgagcgcc gatattaagc atccgaatgg ccttttgcaa cgcttccgga
      661 aacgaccggc ccaaggccat aacttcgccc acacttttca tttctgagcc tatggtatgg
      721 tcgctggact tgagcttcgc cagatcccag cgcggcatct tcaccgccac ataatcaagt
      781 gccggttcga aaaaggcgga agtcgtaccg gtcacagcgt tggtaagttc atgcaggtgc
      841 ttgccaagta ccagcttagc ggccacatat gccagcggat aaccggttgc cttggaagcc
      901 aacgcgctgg agcgactgag ccgcgcattt acttcaatga cccggtaatc gccattctga
      961 ggatttaaag catattgaat gttgcattcg ccaataattt taagatgatt gatagtgcgg
     1021 atggcgactt cgcgcaggcg atgatactcc tcattggtca gcgtctgaga cggcgccacc
     1081 acaatacttt cgccggtatg aatgcccatt ggatcaagat tttccatatt gcagacggta
     1141 atggtattgc ccttggaatc acgaacaacc tcatactcca cctctttcca gcccgccaag
     1201 aactcctcaa tcagtagctg cggcaccgcc tgcaaggctt cagacgcgcg gcgcttcatc
     1261 tcgcggtcat tgtcgaccct ccctgagccg aggccgccca aactatagcc ggcgcgcaac
     1321 attaccgggt agccgattga ttgtgccgcc gctaatgcct cgtccagatt gtaggcaacg
     1381 gcactccgag cggttttggt gccgatttct tccatagagg ccttaaaaag ctggcggtct
     1441 tcggtggttt taatcgtttc taatgaggtg ccggatatcc taatgttcag ccgcttaaat
     1501 acccccttat cgtaaagctc gagccctaaa ttaagggcgg tctggccgcc gaatcccagc
     1561 aggacaatat ccggcttttc ttgctccaaa atcgtcgtaa cggtagcggc atccagcgac
     1621 tcaaaataga ctcggtcggc catatcgctg tcggtttgaa ccgtggcaat attgggatta
     1681 atgagaaccg tagtaatgcc ctcttccttc agcgctttga tggcctggga accggaataa
     1741 tcaaactcgc cggcctgtcc gatacgcagt ccgccggaac caaagatgac ggcttttttg
     1801 ggagtagccg gctgcttcat aggctctcga aaaaccgctt aaacagccag gcagtgtcgg
     1861 ttgggccagg acaagcttca ggatgaaact gcactgaaaa gaagggcttg gaactatgcc
     1921 gaatgccttc caccgaccga tcattcaggt tgcggaacca aacctcccac cctttcggca
     1981 acgaggtctc atcaatcgcg tatccgtggt tttggctggt aatgtagcaa cgcttagtag
     2041 cagcttcgat acagggttga ttatgaccgc gatgcccaaa tttcaattta taggtagttg
     2101 caccggcagc caatgccata acttgcgtcc ccaggcagat gccaaacacc ggcttatcct
     2161 gctccagagc cttgcgggta atcgcaatgg tcgccgcgta atcggtggga tcaccggggc
     2221 cgttggaaag caccacgcca tcatagtctt cggcggtgta atcgtaattg tagggtacgc
     2281 gccggacctg caagccgaga gccagcaagg actgcagaat gttgtttttg gcaccgcaat
     2341 caaccagtac aacggtcttg gtcgcggtcc ccttcggttt atgcacttcc ggctcattca
     2401 ccgatacgaa tttgggctca agacgaactt ttccggcatc aaccgtctcc gcactaataa
     2461 ttccggccat ggtacccgct tcacgcaagt gcttggtaag ggcgcgggta tcaacgctcg
     2521 tcagcaccgg aatgttttgg gaatgcagcc attccaaaaa ggagccttgc gaggcactat
     2581 ggctcccctt aagcgctact tggctcagta tgaccccgct tgcctgaatt ttatccgatt
     2641 cggcgtcatc cggctgcacg ccgtaattac caatcatcgg ataggtaaac accaatatct
     2701 ggttagcata cgaggggtcg gttagcgatt caacgtagcc ggtcataccg gtattaaaga
     2761 ccacctcgcc ggtatactga ccggcctgcc actggggggc caaaccttgg aagaccgttc
     2821 cgtcgctcag gtggagttgc atgggccgat ttacaaaaaa aacgcccctt tcggagcgac
     2881 ttccgtgctg ttgcaaactg tttttgagct gcaattcttg gtccatatgc atccgtttga
     2941 atgtaccaaa aaggtaggtt aaacgcaagt ggtagcggtc cggtcgtcgc ttcgcgctca
     3001 tgtcattatt tgtatgtacg ccttataatg caggtaccat gaaacgctat tatgagtaac
     3061 atcattaccc tgcccggctt gattgatccg catgttcatc tgcgcg
//
LOCUS       DASVGB010000075         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_918229, whole genome shotgun sequence.
ACCESSION   DASVGB010000075 DASVGB010000000
VERSION     DASVGB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918229"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..340
                     /gene="rplX"
                     /locus_tag="VF272_02235"
     CDS             20..340
                     /gene="rplX"
                     /locus_tag="VF272_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7259727.1"
                     /translation="MKLRKGDNVIVITGKDKGKTGEITAVIPKTSKVVVNGVNIAKRH
                     TKPSNKIPQGGILELTKPIDAGKVMAVDPQTGIPGRIGYRINPDGSKERIFKTSKRKK
                     VKKA"
     gene            345..890
                     /gene="rplE"
                     /locus_tag="VF272_02240"
     CDS             345..890
                     /gene="rplE"
                     /locus_tag="VF272_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7259728.1"
                     /translation="MNRLRRKYQDEVRPALKKELGYKNVHQIPNIEKIVINSGVGRAV
                     SDAKHLEMVTGTIRKVTGQSPVTTVAKNSIAGFKLREGNKIGVMVTLRGERMYSFLDR
                     LVALVLPRIRDFRGISVKACDAQGNYSIGLNDQSVFPELTFEETSNRHGIQVNIVTTA
                     QSPEESKRLLELMGFPFQRSS"
     gene            891..1076
                     /locus_tag="VF272_02245"
     CDS             891..1076
                     /locus_tag="VF272_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX7259729.1"
                     /translation="MARLALINKANKTPKYSTRKVNRCKLCGRPRGYMRQFSMCRICF
                     REHASRGQIPGVTKASW"
     gene            1089..1478
                     /gene="rpsH"
                     /locus_tag="VF272_02250"
     CDS             1089..1478
                     /gene="rpsH"
                     /locus_tag="VF272_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7259730.1"
                     /translation="MTDPIADMLTRIRNAIAASRTYVVLPHSNIKESIAKILKENGFI
                     AEYKSEKNGIFTSLKLTLNTDGEIPAINDLQRVSKPGRRVYTAASNIPLVLGGRGMVI
                     VSTSGGLMTGREARGKGLGGELICKVW"
     gene            1480..2016
                     /gene="rplF"
                     /locus_tag="VF272_02255"
     CDS             1480..2016
                     /gene="rplF"
                     /locus_tag="VF272_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7259731.1"
                     /translation="MSRVGRQPISIPAGVTVELNDATVTATGPKGSLSVQLLHGITLD
                     QTSEQITVVKAIDDDERQKTFGLMRTLINNMVVGVSAGFQRQLEINGVGYRANVAGNI
                     INLSLGYSHPIAFNLPQGVDAKIERNVITLTGADKQLIGQVAANLRALRKPEPYKGKG
                     IKYVEERIRRKAGKAASK"
     gene            2027..>2248
                     /locus_tag="VF272_02260"
     CDS             2027..>2248
                     /locus_tag="VF272_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003641260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7259732.1"
                     /translation="MSTTVISKTLQRRNRTRSKISGTAERPRLNVHISNRHVTVQLIN
                     DTTGTTLAFATTAKAADATGTLTDKAVWAG"
BASE COUNT          623 a          533 c          581 g          511 t
ORIGIN      
        1 tcgctccgga ggtgctctaa tgaagctgcg taagggcgac aacgtcattg tcattacagg
       61 taaggataag ggtaaaaccg gcgaaataac cgcggtaata ccaaaaacga gcaaggttgt
      121 agtaaacggc gttaatattg ccaagcgcca caccaagcca tcgaacaaaa tacctcaagg
      181 aggtattctc gagctaacca aaccaattga cgctggcaag gttatggcgg tggaccctca
      241 aaccggtatt cccggccgca ttggctatcg cattaaccct gatggcagca aagagcgaat
      301 attcaaaaca agcaaacgca agaaagttaa gaaggcttag cagtatgaat cgattacgta
      361 gaaaatatca ggatgaggtt cggccggctc taaagaagga gctgggctat aagaatgtgc
      421 accagattcc caacattgaa aagattgtta tcaattcagg agtaggacgg gcggtcagcg
      481 acgccaagca ccttgagatg gtgacaggca ctattcgtaa ggttaccgga caatcacccg
      541 taacgaccgt cgccaaaaac agcattgccg ggtttaaact gcgcgaaggc aacaaaatcg
      601 gcgttatggt aacgctgcgg ggtgagcgca tgtacagttt tctcgatcgc ttggtggctt
      661 tggtgttgcc gcgtattcgc gacttccgcg gcatttcagt aaaagcctgc gatgctcagg
      721 gcaactatag tattggccta aacgatcaat cggtttttcc ggaactgaca tttgaagaaa
      781 cttcaaatcg ccacggcata caagtaaaca ttgttactac ggcgcagtcc ccagaagaga
      841 gtaagcggtt actagagtta atgggatttc cctttcagag gagcagttag atggcacgac
      901 tagcattaat taataaagcc aataaaaccc ccaagtattc aacccgcaag gttaaccggt
      961 gtaagctatg cggccgtccg cgcggctaca tgcgccagtt ttcaatgtgc cggatttgtt
     1021 tccgtgagca tgccagccgc gggcagatcc ctggtgttac taaggctagt tggtaaagga
     1081 ggcatagtat gactgatcca attgcagaca tgttaacgcg aattcgcaat gctatagctg
     1141 cttcccgcac gtacgtggtg ttaccgcact ccaatatcaa ggaaagcatt gcgaagattc
     1201 tcaaagaaaa cggctttatt gctgagtata aatccgagaa aaacggcatt tttacctccc
     1261 tcaagcttac cctcaatact gacggcgaga tcccggcgat taacgattta cagcgcgttt
     1321 ccaagcctgg gcgccgggtt tacactgctg ccagcaatat tcctctggtt cttggcggcc
     1381 gtggtatggt gatcgtttca acctccggcg gtctgatgac tggccgcgag gcgcgcggca
     1441 aaggcctcgg cggtgaacta atctgtaagg tgtggtaaga tgagtcgcgt aggacgtcaa
     1501 cccatctcaa tcccggccgg cgtaaccgtt gagctgaatg acgcaaccgt gacggctacc
     1561 ggaccgaagg gaagtttatc ggtgcagctg ctgcacggta ttacgcttga tcaaacctct
     1621 gagcagatta ctgttgtgaa agccattgat gatgatgagc gccagaagac ttttggtctc
     1681 atgcgcaccc ttattaacaa catggttgtt ggagttagcg ccggtttcca acggcaatta
     1741 gagattaatg gcgttggtta tcgcgctaat gtggccggta atatcattaa tctttcgctc
     1801 ggttattcgc atccaattgc atttaattta ccgcaaggcg ttgatgctaa gattgagcgc
     1861 aatgttataa cgcttacagg tgctgataaa cagttgatag ggcaggtggc agccaactta
     1921 agggcactgc gcaagcctga gccgtacaag ggaaaaggta ttaaatacgt tgaagagcgg
     1981 attcgccgca aagccgggaa agcggcttca aaataaggat tagattatga gtactactgt
     2041 tatatccaaa accctacagc gtcgcaatcg cacccgttcc aaaatcagcg gaacggccga
     2101 acggccgcgc ttgaatgtgc atattagtaa ccgccacgtg accgtgcaac tcattaacga
     2161 caccactggt acaacattgg catttgcaac caccgctaaa gcagcagatg ccacaggcac
     2221 cttgaccgat aaagcggtct gggccggc
//
LOCUS       DASVGB010000076         8500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1252639, whole genome shotgun sequence.
ACCESSION   DASVGB010000076 DASVGB010000000
VERSION     DASVGB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252639"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF272_02265"
     CDS             complement(<1..287)
                     /locus_tag="VF272_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019491402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEX7259733.1"
                     /translation="MTEAVITKETKKQGAAQEVNKDEKSARLKAVDLAVSQIEKQFGS
                     GSIMKLGDAHKIDVKTIPSGSMSLDLALGGGYPQGRVIEIYGPESSGKTTLA"
     gene            502..711
                     /locus_tag="VF272_02270"
     CDS             502..711
                     /locus_tag="VF272_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259734.1"
                     /translation="MAYEATSPAPGNEVSTPRATAGQPAHTVSSTNQMAQPEIPTGAL
                     LISVGAVLVLSILILYFMVRKARSG"
     gene            complement(745..1242)
                     /locus_tag="VF272_02275"
     CDS             complement(745..1242)
                     /locus_tag="VF272_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX7259735.1"
                     /translation="MAKDFSFDVVSDFDPGEMTNAVDQAQRELSQRYDFKGTAAKLEF
                     SEGKTGVQIEGDSRNQIDAIIDMLQSKLIKRGISAKVLDLSSEAIESGMIVRRSVPFK
                     KGLDQEKAKKLTKLIREAYPKAKAQIQGEEVRVSSTSKDDLQGIMNHLKAQELDFPLN
                     FQNFR"
     gene            complement(1261..2235)
                     /locus_tag="VF272_02280"
     CDS             complement(1261..2235)
                     /locus_tag="VF272_02280"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEX7259736.1"
                     /translation="MSLGPKASAKETTPSVGQILKNQRLKREFTLKDAEQATRIRGKY
                     LLAIEADDYDNLPDNVYTRGFIQAYARYLGLATGKIINQYTEERGKQQRPAKAPRRLS
                     VPRFILTPRVVVLFLSLILGFSAAAYLAYQLSALTAPPKLTITNPAKDQVVHGSLITV
                     SGNVAGGADAYVNDSPILVDGRGNFTNVIALQDGVNTIKVSAKNRLGKTSTITRNILA
                     HVPKTDPANSLPTAPFDGVAVKIQVQNVAATITIEVDGKKTFQGIMLPGTTQTFKGAS
                     TITVSTGDAGATSLSVTNAKVAAKDLGIIGKSGEAKSGFQFDKDTQFQ"
     gene            complement(2219..4345)
                     /locus_tag="VF272_02285"
     CDS             complement(2219..4345)
                     /locus_tag="VF272_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX7259737.1"
                     /translation="MARRKSKKNRIPSLSFRPNLSEGVAREIVAVLLVALAFILVFAM
                     FDFGGSLAVGMFTGLRYLFGFGAFLIPMLFLLLAFMIFQPAKYEVQAHNYIGFTGFLV
                     ILAGLLHIAVDADGALELAQQGMGGGVVGLGVAQFFLTILNVPASAIILTALLIIFTI
                     VAANARLSDLVAALMGKFRRPAPKEELTINDPAAVSNQLEIKGALNVGNSKSSKPAKA
                     PEVALTSVADPEWTMPSIELLSKSTNKPDAGNVKENAGIIQNTLSSFGIEVAMGEVNV
                     GPTVTQYTLKPASGVKLTKITGLEHNLALALAAPAIRVEAPIPGKSAVGVELPNKAIA
                     QVRMRQLMQTPEFENAKSPLTFLLGQDVAGKPAVADLAVMPHLLIAGATGAGKSVMIN
                     SLLTSLLYRNSPAQLKLILVDPKRVELNLYGEIPHLLTPVIVEAEKTISALKWAVAEM
                     ERRLKLFAEVGKRNILEYNTSSPEESMPYIVIVIDEMADLMMVAAGDVEALIVRLAQL
                     ARAAGIHLVLATQRPSVNVITGLIKANIPARIAFRVASNVDSRTILDMGGAEKLLGGG
                     DMMFSSADTPKPRRIQGVYTDETETKPVTDYLRSARQPQYNEEVLAQPVSVGGGGGGG
                     GDFGGVDDDLFEEAAETVIRTGKASASLLQRRLRVGYARAARLLDLLEERGVVGQADG
                     ARPRQVLVSSLEESAGEEASSDEFRA"
     gene            complement(4508..6451)
                     /locus_tag="VF272_02290"
     CDS             complement(4508..6451)
                     /locus_tag="VF272_02290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX7259738.1"
                     /translation="MENQPKPAAGGDSSETPKPGASRPSAKPPRGGSGGGRSRRRPQN
                     GPVLKPLSGPIIPVNKSVFNGTGGTQATQVKERKANQITREPKIRIVPLGGLGEVGKN
                     MMAVEYDNDMIMIDMGFAFPDELQPGIDYIIPDTTYIEKNIHKLRGIVITHGHMDHIG
                     AAGYVLPKFHVPIFGTKLSLAMVEKQIEEFKLKAAPDFRVLDPDKHERVQLGAAFNIE
                     LVRVTHSIPDAAAVVIRTPIGTLIHTGDWRLDTDPIDGKQMDLTRFEELGKDKVLLLM
                     SDSTAVERLGSTPTEKQIEATFDEIMNRASGRVIISTFSSSLTRVQLIINSTHKAGRK
                     LAFIGRSMLANVELAVKLGYIKVPPGLITRVQDTAKNTDDQTVILCTGSQGEVNSALS
                     RMSTGDHPTVKIKAGDTVVLSSNPIPGNEKAVVNSVDALLREGARVYQHAYRDLDDIG
                     ILHVSGHAPQDDLAQLITLVKPTYFMPLHGEFHMLVRHAELAVKAGVIAANSFVLDNG
                     DVLEITGNQAKKVDRVPSGIILIDGSGVGDVENLVLRDRLALGSDGMVVVIATVDRKS
                     GRLLTSPDIISRGFVHMKENEDLIGRLRQEIRKSFERRNKTRAVDWGKFKMSLRDDIA
                     DFLYGKTKRNPMILPVINEVDAK"
     gene            complement(6467..7051)
                     /locus_tag="VF272_02295"
     CDS             complement(6467..7051)
                     /locus_tag="VF272_02295"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7259739.1"
                     /translation="MTKQQKLDSLAQRIIERNITPNLASQATQLVMGEINPDADVVFI
                     GEAPGAKEDQLGRPFVGAAGTFLDEMLHSINFQREDVYITNIVKYRPPGNRDPLPEEI
                     AAFKPFLLEQLAIIQPHLVVFLGRHAMSVFLPELKISQVHGRAFRRGKQVFMPLYHPA
                     AALYNGGMRNALLEDFAGIPQVLAQLNNQQTTTT"
     gene            complement(7082..>8500)
                     /locus_tag="VF272_02300"
     CDS             complement(7082..>8500)
                     /locus_tag="VF272_02300"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7259740.1"
                     /translation="AQPKSYELHHHNKEAEAVLKDYLKDRLGEAVRHENQYLRHDAIK
                     NLRHEVVDKFGGEDGKFDSDDLETAFEKVIDAEIRRAILHDKARPDGRGPEAIRPISA
                     QIGLIPRTHGSGLFTRGTTQVLNITTLASTSYAQILDTMEMDTTKRYMHHYNFPGYST
                     GEVRPMRSPGRREIGHGALAERALIPVIPAVEDFPYTIRTVSEAVSSNGSTSMASVCG
                     STLSLMDAGVPIKKPVAGIAMGLVLGEKNYVILSDIQGAEDFAGDMDFKVAGTDDGIT
                     ALQMDIKVKGITADIMKKALEQALGGRAHIMAKMMEVIDAPRPELAPHAPRISKIMIH
                     PDTIKDVIGKGGETINKIIAETGVEIDIEDSGLVMIASTSAEAAKDAIAWVQRLTTRP
                     EVGKIYTGRVVKIMEFGAFVEVMPGKDGLVHISQIADKRIDKVTDVLNEGDEVTVKLM
                     EIDDKGRLNLSMKAAKAEAPSE"
BASE COUNT         2035 a         2279 c         2047 g         2139 t
ORIGIN      
        1 gccagcgtgg ttttaccgct tgattccggt ccgtatattt cgataacgcg gccctgggga
       61 tatccgccgc cgagggcaag atccagcgac atgcttccgc ttggaatggt tttgacgtcg
      121 attttatgag cgtcgcccag cttcataatc gagccgctgc caaactgctt ttcaatttgg
      181 cttacagcta aatcaacagc ttttagccga gcgctctttt cgtctttatt cacttcttgt
      241 gccgccccct gcttcttggt ttctttagtg ataactgctt cggtcattgt atcatgtccc
      301 tcggttagct tggtgttgat agctactata acgaagagtt atgaattatt tcttaacgcc
      361 tctgctgtag ctgttattgt atgtgattta agcataaccg aacaaaaggc gaactgtcaa
      421 gcacaagggt tctccgcacc tccaccaggg ctatgagata agctataatg atgctatgaa
      481 cgtgcgggtt accaacaact aatggcatac gaagccacct cccccgcgcc agggaacgag
      541 gttagcacgc cgcgagcaac agctgggcaa cctgcccata ctgtttccag caccaaccaa
      601 atggcccaac cggaaatacc caccggggca ttgcttatta gtgttggcgc ggttttggta
      661 ctatcaattc ttattcttta ttttatggtg cgtaaggcca ggtcaggcta aaagtagcga
      721 gtttaaagct tgccgagtaa cttgctaacg gaagttttgg aagttcagcg ggaaatctaa
      781 ttcctgggct ttgaggtgat tcattatccc ctgcaaatca tccttggaag tactggaaac
      841 ccgaacctct tctccctgga tttgggcctt agctttagga taggcttcgc ggattaactt
      901 ggttagcttc ttggcctttt cttgatctaa ccccttttta aacggcacgc tgcgacgcac
      961 aatcatcccg gactcaatcg cttcggatga caaatcaagc actttggctg aaataccgcg
     1021 ctttatcagc ttgctttgga gcatatcgat aattgcatca atttggttac ggctatcccc
     1081 ttcaatttgc accccggtct tgccttcgct gaattccagc ttagcggccg tgcctttaaa
     1141 atcgtagcgc tgcgaaagtt cgcgctgagc ttgatcaacc gcattggtca tttcgcccgg
     1201 atcgaaatcg ctgactacat cgaatgaaaa atcttttgcc ataggcttag tataccaagc
     1261 ctattggaac tgcgtatctt tatcgaactg aaagccgctt ttggcttcac cgcttttgcc
     1321 aataataccc agatccttgg ccgccacctt agcattggta acacttaaac tggtggctcc
     1381 cgcatcgcct gtagaaacgg taatggtgga ggcaccctta aaggtttgag tggtaccggg
     1441 aagcatgatg ccctgaaagg tctttttacc atcaacctca atagtaatgg tggcagcaac
     1501 attttgtacc tgtattttta ccgccacccc gtcaaatgga gcggttggca aactatttgc
     1561 ggggtcggtt ttagggacgt gcgctaatat attgcgggta atcgtggagg ttttcccaag
     1621 ccggttctta gcgctgacct taatagtgtt tacgccgtcc tgcagggcta tcacattggt
     1681 aaagttgcct ctgccatcaa ccagaattgg cgaatcgttg acataagcgt cggctccacc
     1741 cgccacattg ccggataccg taattaaact gccatgaact acttgatcct tagcaggatt
     1801 ggtaatggta agtttagggg gggcggttaa ggcgctaagc tgataagcca agtaggctgc
     1861 agccgagaat ccaaggataa ggctcaaaaa caaaacaacc actcgcggcg tcaggataaa
     1921 ccgaggaacc gacaggcgcc tcggcgcttt cgccggccgt tgctgtttac cgcgctcttc
     1981 tgtgtactgg ttgataattt taccggtagc aagccccagg taccgggcat acgcttgaat
     2041 aaagccgcgg gtatagacgt tatcgggaag attatcgtaa tcatcggctt caattgccag
     2101 taagtattta ccgcggattc gcgtggcctg ttcggcgtct tttaaggtga attcgcgctt
     2161 gagccgctga tttttaagaa tctgcccgac actaggtgtg gtttctttgg cagaggcttt
     2221 aggccctaaa ctcatcagag ctcgcttctt cacctgcaga ttcttctaaa cttgagacca
     2281 acacttggcg gggacgagca ccatcagcct ggcccacaac accgcgttct tctagcagat
     2341 caaggagtcg tgcggcccgg gcatagccta cccgcaaacg acgctgcaat aacgaagccg
     2401 aagccttgcc ggtacgaata acggtttctg cggcttcttc gaacaagtcg tcgtcgacac
     2461 cgccgaagtc gccaccacct cctccaccgc cgccaacgct aaccggctgc gccaagacct
     2521 cctcgttgta ttgaggctga cgggcgctgc gcaagtaatc ggtaaccggc ttagtctcag
     2581 tttcatccgt ataaacacct tggatacgcc gcggcttagg cgtgtcggca ctagaaaaca
     2641 tcatatcgcc gccgccgagc agtttttcgg cgccacccat atcaagtatg gtgcgtgaat
     2701 cgacattcga tgctacccgg aacgcaatac gggccggaat gttggcttta atgagcccgg
     2761 taatcacatt cacgcttggc cgctgggtag ccagaaccag gtgtataccg gctgcccggg
     2821 ccaactgggc cagacgaaca atcagcgcct caacatctcc ggcggcgacc atcatgagat
     2881 cagccatctc atcaataaca ataacaatat agggcatgct ttcttcagga cttgaggtgt
     2941 tgtattccag aatattacgc ttgccgacct cggcaaacag tttcaagcga cgctccattt
     3001 ccgctaccgc ccacttcaag gcagagattg ttttttcagc ttcgacaatc accggtgtga
     3061 gtaagtgcgg gatttcaccg tacaggttaa gctctacccg cttaggatct accaaaatca
     3121 acttaagctg ggccggcgag ttgcgataca gcaaagacgt caacaggcta ttgatcatca
     3181 ccgacttacc ggcgccggta gctccggcaa tgagcaaatg cggcatgacc gccaggtcgg
     3241 caaccgccgg cttcccggca acgtcttgcc ccaataagaa cgttagcggc gacttggcat
     3301 tttcgaattc gggagtttgc atcagctggc gcatccgaac ctgtgctata gccttattcg
     3361 gcagctcaac acctaccgcg ctcttgccgg gaatcggtgc ctctacgcgt atggctggag
     3421 ctgccaaagc tagtgccaag ttgtgctcaa gaccggtaat ctttgttagt ttgacgccgc
     3481 tcgccggttt taaggtgtac tgggttaccg ttggtcctac attgacctcg cccatcgcca
     3541 cctcaatacc aaaactggac agggtgtttt gaatgatacc ggcgttctct tttacgttgc
     3601 cggcatcggg cttgttggtt gatttgctta acagctcaat ggaaggcatt gtccattcgg
     3661 gatcggccac gctggtaagc gcaacttccg gtgctttggc tggcttgctg ctcttgctgt
     3721 tgccgacatt aagcgcaccc ttaatttcca gctgattgct caccgcagcc ggatcgttga
     3781 tagtgagctc ttctttcggt gctggccgtc gaaacttgcc catgagcgca gctacaagat
     3841 cgcttaaacg ggcattcgcc gccacaatcg taaagataat taatagtgcc gtgagaataa
     3901 ttgccgaagc cggaacattc aagattgtta aaaagaattg ggcaacccca agacccacaa
     3961 caccgcctcc cataccttgc tgcgccagct ctagggcacc gtcggcatca accgcaatat
     4021 gcaacaatcc ggccaagata accaggaaac cggtaaaacc tatgtaatta tgagcttgaa
     4081 cctcatactt cgccggctga aaaatcataa aagctagcag taagaacaac ataggtatta
     4141 agaaagcgcc aaagccaaac aagtagcgta agcctgtgaa catcccgaca gctagcgacc
     4201 cgccgaaatc aaacatcgcg aacactagaa taaaggccag ggccactaac agcacggcca
     4261 caatctcacg cgctacgcct tcactaagat tagggcggaa gctgagcgaa ggaatacgat
     4321 ttttcttgga tttacgcctg gccatagtgc ctctattata ccgcttttgc tatctgaggg
     4381 ctagataagg agtaggctta ccgggtaata actttgtggc ataggattga ttgactgaag
     4441 ccgtctgata tatgcctaca aaagttttac cacggcgcac cctatcaagc atgtagctgt
     4501 cttccttcta cttagcatcc acctcattga taaccggcag aatcattggg ttacgcttgg
     4561 ttttaccgta caaaaagtcg gcaatatcat cgcgcaaact catcttgaac ttgccccagt
     4621 cgactgcacg ggttttattc cggcgctcaa atgacttacg gatctcttgc cgcaggcggc
     4681 caatcagatc ttcattttct ttcatatgaa caaagcctcg ggaaatgata tcggggctgg
     4741 tcaacaggcg tcccgacttg cggtcaaccg tagcgataac cacaaccata ccgtccgagc
     4801 ccagcgccaa gcggtcacgc agtaccaggt tttccacgtc ccctactccc gagccgtcaa
     4861 tcaatataat cccgctcggt acacgatcaa cctttttagc ttgattcccg gtaatttcca
     4921 gaacgtcgcc gttatccaac acaaagctat tggcggcaat caccccagcc ttaactgcca
     4981 gttcggcatg gcgaaccaac atatgaaatt cgccatgcag cggcataaaa taggttggct
     5041 ttaccagcgt aattaactgc gccaggtcat cctgaggggc atgcccggac acgtgcagaa
     5101 tgccaatatc atccaggtca cgataggcgt gctgataaac ccgtgcgcct tcacgcagta
     5161 acgcgtcaac cgaattaacc acggcttttt cattacccgg aatcgggttt gaacttaaga
     5221 ccacggtgtc accagcctta atcttaacgg tgggatggtc gccggtggac atgcgcgaaa
     5281 gtgccgaatt cacctcgccc tgcgatccgg tacagagaat caccgtttga tcgtcggtat
     5341 tcttagcggt atcttgaacg cgagtaatca gccccggcgg cacttttata taccctaact
     5401 taaccgccag ctcaacgttt gcgagcatac tccggccgat aaaggccagc ttgcgtccgg
     5461 ccttatgcgt tgaattaata attaactgta cgcgggtaag agagctagag aacgtagaaa
     5521 taattacccg gccgctggcc cgattcataa tttcatcgaa ggtagcttca atctgcttct
     5581 cggtaggagt agaacctaac cgctcaaccg ccgtactatc actcatcaac agcaaaacct
     5641 tgtccttgcc tagctcttcg aaacgcgtaa gatccatttg tttaccatca atcggatcgg
     5701 tatcaagacg ccagtcgccg gtatgaatca aggtgcctat aggtgtacga attaccaccg
     5761 cggcagcatc gggaattgag tgggttaccc ggactaactc aatattaaaa gccgcaccca
     5821 gctggacacg ttcatgcttg tccggatcaa gcacccgaaa gtccggagca gccttcagtt
     5881 tgaattcctc aatttgcttt tcgaccattg caagactcag cttggtaccg aagatcggca
     5941 catggaactt gggcagcacg taaccggcgg cgccaatatg atccatgtgg ccgtgagtaa
     6001 taacaatccc tcgtaacttg tgaatgtttt tttcaatata ggttgtgtcg ggaataatat
     6061 agtcgatgcc tggctgaagt tcgtcgggaa acgcaaaacc catatcaatc atgatcatgt
     6121 cgttgtcata ttcaacagcc atcatgttct tgcccacctc gccgagcccg cctagcggaa
     6181 caatgcgaat ctttggctcg cgagtgattt gattggcctt ccgctcctta acttgagtgg
     6241 cttgggttcc gccggtgcca ttaaataccg atttgttaac cggaattatc gggccggata
     6301 gcggctttaa gacgggcccg ttctgggggc gccgacgtga acgtcctccg ccagagccgc
     6361 cgcgcggagg tttggccgaa gggcggcttg cgcctggttt gggggtctcg cttgaatcac
     6421 cgccagcagc cggtttaggt tgattttcca tatatgtttc cttactttac gttgtggttg
     6481 tttgttgatt gttgagttga gccaaaacct ggggaatacc ggcaaaatct tcaagtagcg
     6541 catttcgcat accaccgtta tacagcgcgg cagccggatg atagagcggc atgaaaacct
     6601 gttttcccct ccggaatgcg cggccgtgca cctgagaaat ttttaactct ggcaagaata
     6661 cactcattgc gtgtcttccc agaaatacca ccaagtgcgg ttgaataata gcgagctgct
     6721 ctaacagaaa gggtttaaag gctgcgatct cttcgggaag cggatcgcga ttaccaggcg
     6781 gacggtactt tacgatgttg gtaatgtata cgtcctcccg ctgaaagttg atactatgca
     6841 gcatctcatc caaaaacgtg ccggcggcac ctacaaatgg ccgccccaac tgatcttctt
     6901 tggcccccgg tgcttctccg atgaagacaa cgtcagcatc ggggttgatc tcccccatca
     6961 ccaactgggt tgcttgggag gccaaattcg gggttatgtt cctttcaatg atccgctgag
     7021 ctaaactatc aagcttctgt tgcttggtca tgctaggcgc taatcgcggc tgatctagcc
     7081 attattcgga aggagcctcg gccttagcag ctttcatgct gaggttaagc cggcccttat
     7141 catcgatctc cataagctta accgtaactt catcaccctc attcaggacg tcggtaactt
     7201 tatcgatccg cttgtcggca atctgggaga tatgaaccag tccatccttg cccggcataa
     7261 cttcgacgaa agccccaaat tccataatct tcaccacgcg gccggtatag attttgccaa
     7321 cctcgggacg agtggtcagg cgttgaaccc aagcaatcgc atccttagcg gcttcagcgc
     7381 tggtggatgc aatcatcacc aaaccgctat cttcaatgtc gatttcgaca ccggtttcag
     7441 cgatgatctt attaatggtt tcaccgccct ttccaatcac atctttaatg gtgtcggggt
     7501 gaatcataat cttggagata cgcggggcgt gaggcgccaa ttcaggacga ggcgcatcaa
     7561 taacttccat catctttgcc ataatatgag cgcggccgcc aagcgcttgc tcgagagctt
     7621 tcttcataat gtcagcggta atacccttca ccttgatatc catttgcagc gcggtaattc
     7681 cgtcatcggt cccggcaact ttgaagtcca tgtcaccggc aaaatcttca gctccttgaa
     7741 tatcactgag aatcacatag tttttctcgc caagcaccag acccatggca ataccggcta
     7801 ccggtttctt tatggggaca ccggcatcca ttaagctgag ggtactgccg caaacgctgg
     7861 ccattgaagt cgaaccgttc gaactaacgg cttcagaaac ggtgcggatg gtgtagggaa
     7921 aatcttcaac cgccggaatt accgggatga gggcccgttc cgccagagcg ccgtggccaa
     7981 tttcacgccg gccaggactg cgcatcgggc gcacctcgcc ggtagagtag ccggggaaat
     8041 tataatgatg catatagcgc ttggtggtat ccatttccat tgtatcgaga atctgagcat
     8101 agctcgtgga agccaaggta gtaatgttca gcacctgggt tgtaccccgg gtaaacaatc
     8161 ctgagccatg cgttcttgga atcagtccaa tttgagcact aattgggcga atagcttccg
     8221 gaccgcggcc atcaggacgg gctttatcat gcagaattgc gcggcggatt tcggcatcga
     8281 ttaccttttc aaaggcggtt tccaagtcgt cagagtcgaa cttgccatct tctcccccga
     8341 acttgtcgac cacttcgtgg cgcaagtttt tgatcgcatc atgacgcaaa tactgattct
     8401 catgacgcac cgcttcgccc aagcgatcct tcaaataatc tttcagtaca gcttcggctt
     8461 ctttattgtg atgatgcagc tcatacgatt taggctgggc
//
LOCUS       DASVGB010000077         6313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1193989, whole genome shotgun sequence.
ACCESSION   DASVGB010000077 DASVGB010000000
VERSION     DASVGB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193989"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VF272_02305"
     CDS             complement(<1..1075)
                     /locus_tag="VF272_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX7259741.1"
                     /translation="MQPPVPASPEEASWEQPLPLPAPAIRRSAWSSRRAVISAAVLIV
                     VASVGLVGMKVVMASQQVVTKNTAGPAPALAGTIDPTKLSGEGDGRINILLLGIGGDG
                     HSGGTLSDTIMVASVDPVHHTVAMLSIPRDLYVRIPGHGASKINAANSWGGPELAKQV
                     VGEVLDLPIHYYMQVDFQGFKQAVNAVGGVDIDNATLLSDAQYPCDNGRGICPFNLPA
                     GDYHMDGVQALKYARCRNGTCGADFGRAARQQQLMIALREKALQLSTLTNPLKISNLI
                     DSIGNHVRTDMQLTELQKMVVAVKAIDSSKMTTKVLDGAPDGLLMSGSRQFPGAGAIL
                     IPKAGAFNYAPIRELAHSLFIDSY"
     gene            1231..1872
                     /gene="lepB"
                     /locus_tag="VF272_02310"
     CDS             1231..1872
                     /gene="lepB"
                     /locus_tag="VF272_02310"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7259742.1"
                     /translation="MQPDFQTPNPPPPAYQPVPEPKTSIIESISAFFSHWFISWIVIP
                     GGMVLLLHFYVFSAYHVVGSSMTPTMHNSDYLIVSKVDKTIAAIKGEPYIPKRNQIIV
                     FHYPKQPELDFVKRVVGLPGERVVVKDCKVTVYNTEQPNGFNPDLTHEISGTCTEGEE
                     FNGAVPNKTIFVLGDNRMPGGSSDSREWGFLPSYDIVGNAVLRLYPLDGFRIF"
     gene            complement(1886..2773)
                     /locus_tag="VF272_02315"
     CDS             complement(1886..2773)
                     /locus_tag="VF272_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7259743.1"
                     /translation="MSKQRGLGRGFDALIPTDVSLDMPASVHPAGKQSIQLVDPAAVK
                     PNPHQPRQHFDENDLASLAASIKEHGILHPPVVSQLAEESYELIAGERRVRAAKLAGL
                     TTIPVIVRSFEEQQKLELALLENVQRSELNPLETAAAYRKLIDEFNLSLDQVGERMGQ
                     ARSTVSNLMRLLQLPPDAQSALQHGKISEAHGRAILALSEPARQAELLAYILQEGWTV
                     RQAEAFVQAGKQGSAKSLKTAVKPAAVAEALQLASELGGYLGAKVSMSPRGKGGRLTI
                     EYRSKEELVRIVDTIKPKQ"
     gene            complement(2770..3561)
                     /locus_tag="VF272_02320"
     CDS             complement(2770..3561)
                     /locus_tag="VF272_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7259744.1"
                     /translation="MTDTNTPHIIALANQKGGVGKTTTAINLAAFLAKSGHAVMVVDL
                     DPQGNTTSGFGIDKQAVDTNLYHVLMDGKHAHEAALRTAFHDIVVLPASSVLAAAEVE
                     LVGIEQREFRLKQVLENLPYEYILIDCPPSLGLLTLNGLVAADSLIIPVQAEFYALEG
                     LSQLLQTVDRVRRALNPQLDLMGVVLTMHNGRTTLSAQVHDEVKRHFPDKVFDTIIPR
                     NVRLAEAPSFGQPISHYDRWSKGGRSYHNLAKEVTKRAKHVKAVK"
     gene            complement(3598..4419)
                     /locus_tag="VF272_02325"
     CDS             complement(3598..4419)
                     /locus_tag="VF272_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX7259745.1"
                     /translation="MPSPKHPLKLHRSVPKPHGFHPTKTWVKHPYIYRYDPSLRVCKR
                     TPIDDSARGVGGYFSVVMAVEKKSPFYARLLRHISKTFRLSPALAPRAFAGVFVIAAA
                     VFTYPFVPALHHRLSHQPNLPQGVQLAKVPPAQPAKADNPTNRVIIPSIGVNTPILES
                     SSLAILDVHEGVWHQTGTLQTSNFVIAGHRFKYLPPNTTTFYNLDKLAAGDVIVIDWV
                     GKRNVYTVAETRTIKATEIGVIAPTAVPQLTIYTCSDKEETERIVVIARPLPKEG"
     gene            complement(4406..5683)
                     /locus_tag="VF272_02330"
     CDS             complement(4406..5683)
                     /locus_tag="VF272_02330"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7259746.1"
                     /translation="MASATGSNPTNNPRMDLLHSSGDNQQVQGSSGPVPVPVASFDSK
                     SEVHADAPAPAPHPSRPAVAAKAPAPPPPPPPRSAPVASSDDPKNNPKKGGGLAKVAV
                     LIAIIIVIVGVGYGVWRFLTIPSSTPSDSSPVTKNLELAPLMDLNQTGKDKVIAVGKT
                     TASREVIFSFTLPTNANVGSANPQIELQPVGTAFTGKPTTSGDPVKANGKDLTLSIPV
                     KDLADGQYHWQARVVISDEPGEWVVFGDDPTIASLLVDATPPPAPVAETADGSAVKAG
                     QVSTTKSQPVFNGTAEPGSTISLVVKPAGTKISTVADASGKWSVAITSDIPSGQSEIN
                     ITAGDAAGNVSEATAFSITVSLAAATPAPAPAPAPAPAPAPAPAPVPAPAPVATTLAP
                     TGDNTMLMSLLSLMVMALSAFGWMKTRRRYAKS"
     gene            complement(5818..6156)
                     /gene="rplU"
                     /locus_tag="VF272_02335"
     CDS             complement(5818..6156)
                     /gene="rplU"
                     /locus_tag="VF272_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7259747.1"
                     /translation="MQAVITTGGKQYLVQADQTLEVDITGDGAKTIAFEPLLVIDGTY
                     VHVGEPVVGNMMVQAAVIGEVKGEKIDVLKFKAKKRVNTKIGHRQRYTQIKIVSIGSS
                     KTAKKPVASS"
     gene            complement(6197..>6313)
                     /locus_tag="VF272_02340"
     CDS             complement(6197..>6313)
                     /locus_tag="VF272_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX7259748.1"
                     /translation="GIPLPFLSYGGTSLLVSLLAIGLLQSIAIRRKKIQFGT"
BASE COUNT         1533 a         1644 c         1599 g         1537 t
ORIGIN      
        1 tatagctgtc aatgaacaaa gaatgcgcca attctcgtat tggggcatag ttaaaggcgc
       61 cggccttggg aattaagatt gccccggcac ctggaaactg acggctgccg ctcataagta
      121 agccgtcggg cgcaccatcc aagaccttgg tcgtcatttt ggaggaatca attgctttga
      181 cggcaacgac cattttttgc aattcggtaa gctgcatatc tgtgcgcaca tgattgccga
      241 tactgtcgat taagttgctg attttgagtg ggttggtaag ggttgaaagc tgcagcgcct
      301 tttcacgcag agcaatcatg agctgttgtt gacgggccgc ccggccaaaa tcagcgccac
      361 aggttccatt gcggcaacga gcatacttca acgcctgaac gccatccata tggtagtcgc
      421 cagccggaag gttgaagggg caaatgccgc gtccattgtc gcaaggatac tgcgcatcag
      481 aaagcagcgt tgcattatca atgtctactc caccgacagc attaaccgct tgtttaaaac
      541 cctggaaatc aacttgcata tagtaatgga tgggcagatc tagaacctcc ccgacaactt
      601 gtttggctaa ctccggcccg ccccagctat ttgccgcatt gattttacta gcaccatggc
      661 cgggaatgcg tacatacagg tcgcgcggaa tactcagcat cgcaacggtg tgatgcacgg
      721 gatcgacgct cgcaaccata atggtatcgc ttaatgtgcc gccgctatga ccgtcgccgc
      781 caatgccaag cagtaaaata ttgatccgac cgtcaccttc tccgctcagc ttggtaggat
      841 ctatggttcc tgctagtgcc ggggccgggc cagccgtatt tttggtaacc acctgctgcg
      901 atgccatcac caccttcatg ccaaccaagc cgacactggc caccacaatc aataccgccg
      961 cagaaataac cgctcggcgg ctcgaccaag ctgaccggcg aatagccggt gctggcagcg
     1021 gaaggggttg ctcccagctt gcttcctcgg gagatgccgg tacgggaggt tgcatagggg
     1081 gagttggcat cgcctggcga ctgagcccat caaggctccg gattggggat cggttctcca
     1141 ttactatcca ctatactgaa tcagagcata agcgtaaagg ttgctgcagg gaggtaaact
     1201 ctttatactg gggaagtaat aaaggcaatc atgcagcccg atttccagac tccgaatccg
     1261 ccgcctcccg cctatcagcc cgtcccggaa ccaaaaacat ccattattga atcaatcagc
     1321 gcttttttct cccactggtt tattagttgg attgtaattc ccggcgggat ggttcttttg
     1381 ctgcatttct atgtctttag cgcttatcat gtggtgggat caagcatgac cccaacgatg
     1441 cataattccg actatcttat tgtctctaaa gttgataaaa caattgcagc aattaaaggc
     1501 gaaccatata tccccaagcg caatcagatt attgtctttc attaccccaa gcagccggaa
     1561 cttgattttg taaaacgcgt agtggggctt ccgggtgaac gcgtggttgt aaaagactgc
     1621 aaggttacgg tatacaatac cgaacaaccg aatgggttta atcctgattt gactcacgag
     1681 atatccggaa catgcactga aggagaagag tttaatgggg cggtgcccaa taaaacgatt
     1741 tttgtactgg gggacaatcg tatgcccggc ggatcatccg attcccgcga atggggcttc
     1801 ctgcctagct atgacattgt tggtaatgcc gttctccggc tctatccgct tgatggcttc
     1861 cgtatttttt aaatagaatt cggcactatt gtttgggttt aatcgtatcg acgatacgaa
     1921 ccaactcttc cttagaacga tactcaatcg tcaaacgtcc gcccttaccc ctagggctca
     1981 tactgacctt ggcgcctaaa taccctccca attcgcttgc tagctgaagc gcttcagcta
     2041 cagccgctgg cttcacagct gtttttaacg acttggcaga accctgcttg ccggcctgaa
     2101 cgaaggcttc ggcctggcgt acggtccacc cctcctgcag tatatatgct aaaagttcgg
     2161 cttgcctggc tggctcgctg agtgccagaa tcgcgcgtcc atgagcctcg ctgattttgc
     2221 catgctgtaa agccgactga gcatcaggcg gtaactgcag taagcgcatg agattggaaa
     2281 ccgtagagcg agcctgcccc atacgttcgc cgacttgatc taatgataga ttgaactcat
     2341 caataagttt gcggtaagca gctgctgttt ccagcggatt cagctcgctg cgctgcacgt
     2401 tttctagcaa cgctaattct aacttttgct gttcttcaaa actacggaca attaccggaa
     2461 tagttgttag tccggctaat ttagccgccc gaacccgacg ctccccagca atgagctcat
     2521 atgactcttc agccaactgg ctgaccaccg gaggatgaag aatgccatgc tctttaatcg
     2581 aggcggccaa acttgccaaa tcattttcat caaaatgctg acgaggctga tgcggatttg
     2641 gcttcaccgc tgcagggtca accaactgga tcgattgctt cccggcagga tgaaccgaag
     2701 ccggcatatc caggcttaca tcggtgggta ttaatgcatc aaatcctcgc cctaaaccgc
     2761 gctgcttact catttcaccg cctttacgtg cttagcgcgc ttggttactt ctttggccaa
     2821 attatgatag ctgcgtccac ccttactcca gcggtcatag tggctaatcg gctggccaaa
     2881 actgggtgcc tcagccaaac gaacgttgcg cggaatgatt gtatcaaaaa ccttatccgg
     2941 aaaatgacgc tttacctcgt cgtgtacttg ggcactcaag gtggtgcggc cattatgcat
     3001 ggtcagcacc acacccatca ggtcaagctg cgggtttagg gcccggcgga ctcgatccac
     3061 ggtttggagc agttggctta agccctcaag cgcataaaac tcggcctgaa ccggaataat
     3121 gagcgaatcg gccgcaacca aaccgttcaa ggttaaaagt ccaagcgacg gcgggcaatc
     3181 aattaaaata tactcatagg gaagattttc cagtacttgc ttaagccgaa attcccgttg
     3241 ctcgatacca accaactcca cctcggcagc agcgagtacc gatgaggccg gcagcaccac
     3301 aatatcgtga aacgcggtac gcagcgccgc ttcatgagcg tgcttaccgt ccattaatac
     3361 atggtagagg ttggtatcga cggcttgttt atcaatcccg aacccactgg ttgtattgcc
     3421 ttgtggatca agatcaacca ccattaccgc atgtccgctt tttgccagaa aggcagctaa
     3481 attaatagcc gtggtagtct tccctacgcc ccctttttgg ttggctaatg caatgatgtg
     3541 cggtgtgttt gtatcagtca tatcataacc attttaccat ggagtagtag gtgggttcta
     3601 accttctttg gggagtggcc gagcaatcac cacaatccgt tcggtttctt ccttgtcgct
     3661 acatgtatat attgtaagct gcggcacggc cgttggcgca ataacaccaa tttcagtggc
     3721 ttttatggtt ctggtctctg ccaccgtata gacattccgc ttaccaaccc aatcgatcac
     3781 aataacatcg ccggccgcca gtttatcgag gttataaaag gttgtagtat tcggcggaag
     3841 atacttaaac cgatggccgg caatcacaaa attactggtt tgaagcgtgc cggtttgatg
     3901 ccatacgcct tcatgcacat ccaaaatcgc caagcttgag ctttccagaa tcggcgtatt
     3961 tacaccaata ctgggaataa taacccggtt ggtggggttg tcagccttgg ccggctgagc
     4021 cggcggaact ttcgccagtt gcaccccttg aggaagatta ggctgatggc tgagccggtg
     4081 gtgcagtgcc ggaacaaacg gatacgtaaa aaccgcagca gcaatcacaa acactccggc
     4141 aaaggcccta ggggccagag ccggactgag acggaaggtc tttgaaatat gtcgaagcag
     4201 cctggcgtaa aacggagact ttttctccac tgccatcaca acactaaaat agccgcccac
     4261 gcctcgagcg gaatcgtcaa tcggcgtccg cttacaaacc ctgagactgg ggtcgtaacg
     4321 gtatatgtaa ggatgtttga cccaggtttt cgtgggatga aatccatgag gttttggaac
     4381 tgaacgatga agtttcagcg gatgcttagg acttggcata ccggcgccgt gtcttcatcc
     4441 atccgaatgc agacaacgcc ataaccataa gtgagagcag gctcatcagc atcgtgttgt
     4501 cgccggttgg cgccaaggtc gttgctacgg gagcgggggc tggcaccgga gcaggagcag
     4561 gagcaggagc aggtgcaggt gccggggctg gagcgggagc tggggttgca gcggccaagc
     4621 ttaccgtaat agagaaggcc gttgcctcgc ttacattgcc ggcagcatcg ccggcagtaa
     4681 tattgatctc gctttgaccg ctgggaatat cactggtaat cgctacagac catttgccgg
     4741 aagcgtcggc gaccgtacta attttagtac ccgccggctt aaccactaag gaaatcgtgc
     4801 tgccgggttc ggccgtacca ttaaataccg gttggctttt ggtagtgctg acctgaccgg
     4861 ctttcacagc gctcccgtcg gcagtttcgg ctactggggc aggtggtggc gtagcatcaa
     4921 caagcaagga ggcaatggta ggatcatcgc caaagacaac ccactcccct ggctcatcgc
     4981 taattactac ccgtgcctgc cagtggtatt gcccgtccgc taaatcctta accggaatgc
     5041 taagggtcaa gtcttttccg ttggccttaa ccgggtcacc gctcgtagtg ggcttgccgg
     5101 tgaatgcggt accaaccggt tgaagctcaa tctgcggatt ggcagagccc acattcgcat
     5161 tggttggcag agtaaagcta aaaatgacct cacgagaagc ggttgttttg ccaaccgcaa
     5221 taactttatc cttgccggtt tgattcagat ccatgagcgg agcaagttcg aggttttttg
     5281 taaccggaga agaatctgaa ggcgtcgagc tgggaatcgt tagaaaacgc caaactccat
     5341 accctacacc gacgatcaca attatgatag caataagaac ggcaactttc gccaaaccgc
     5401 cacccttttt aggattattc ttcggatcat ctgatgaggc aaccggcgca ctgcgcggcg
     5461 gaggcggagg gggcggagct ggagccttgg cggctactgc cggacgtgaa ggatgcgggg
     5521 caggcgctgg agcatcggcg tgaacttcag acttggaatc aaacgatgcg accggaaccg
     5581 gtaccggccc ggaagaacct tggacttgct ggttgtctcc gctgctatgg agcaagtcca
     5641 tgcgcgggtt atttgttgga ttactgccgg ttgctgatgc cattttccac ctataggtta
     5701 cacacctgtc tcgtaatcag tataactata agcacaagca ttaaacaagt attttattga
     5761 acggacagca aaataccggc ttggtgccgg caaaaactta cgaatacttg caatgaatta
     5821 gctggaggcg acgggttttt tcgcagtttt ggatgatcca atcgaaacaa ttttgatttg
     5881 agtgtaccgc tggcggtggc cgattttggt gttcacccgt tttttagctt taaacttcaa
     5941 aacgtcgatc ttttcacctt taacttcacc aatcaccgct gcctgcacca tcatattccc
     6001 caccacgggc tcaccaacat gcacgtacgt gccatcaata accaggagcg gctcaaacgc
     6061 tatggtttta gcaccgtctc cagtgatatc tacttcaagt gtttggtccg cttgaaccaa
     6121 atactgtttt ccgccggtag taatgactgc ttgcataatt catgtagtat acccggaacc
     6181 agccgatctg taaaccttag gtgccaaact gaattttctt acgccggatg gctattgact
     6241 gcagtaagcc aatggctaaa agagaaacca gcaagctggt gccgccgtag gataaaaacg
     6301 gcagcggaat gcc
//
LOCUS       DASVGB010000078         4112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_717074, whole genome shotgun sequence.
ACCESSION   DASVGB010000078 DASVGB010000000
VERSION     DASVGB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717074"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF272_02345"
     CDS             complement(<1..397)
                     /locus_tag="VF272_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259749.1"
                     /translation="MMATAIIGILATLVSGFYVDRLIDYARNDTLIILQSNTKQALES
                     LGRDIKSARTIQSANQWPDPNGPGGNQYGWSSNTGSPSVLVLAVPAVNSSGGLIYADA
                     SHSSLQTNDVIYYVDGVKKLLYRRVIANPV"
     gene            complement(436..837)
                     /locus_tag="VF272_02350"
     CDS             complement(436..837)
                     /locus_tag="VF272_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259750.1"
                     /translation="MKILRSQNGFTLVELVVTAVFVTVASAAIITIFIVVGKLNKQAR
                     NMAVATALAEQKLEIYRDAGYHAIPTGAPAETFTGALPANFGSPKSAVANVTTPQAGL
                     KRIDIIITYTDDKRTKNVQISTLMTQRGINR"
     gene            complement(954..1403)
                     /locus_tag="VF272_02355"
     CDS             complement(954..1403)
                     /locus_tag="VF272_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7259751.1"
                     /translation="MNTLKKQGGFTLLELLIVIVIIGILAVLIIPNLVSGPQRARDSQ
                     RKSDLRNIKTALETYYNDNNSYPTAGGATCVPNAGGCLGTVLTAGSTPYMKTVPNDPK
                     GGANYTYEPSPGSCAAGACVSYELRATLENSKDSQATAGVYTVNSAN"
     gene            complement(1525..2745)
                     /locus_tag="VF272_02360"
     CDS             complement(1525..2745)
                     /locus_tag="VF272_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7259752.1"
                     /translation="MATYNFTARDHSGTIQKGSLVANDQAAAAASLLERDLVPILIKV
                     QEAKTKGKGLQLGGLKLGGRVKLEDKVIFSRQFATMINAGVPLTQALSMLGGQTTNKH
                     FRDVINDAAKQVEGGSTLSNALAGHPDVFSQVYVNMVKAGEAGGLLDQVLERLAVQQE
                     KDAAVVAKVRSAMIYPGILLSVTVGAFVFLMTYLVPRMSVIFESLGTELPWYSKLMLA
                     MSDSLVNYGLFLLIGLVVLAVVGFRFVHTIRGKTIFDSILIRMPIFGPIIVKVNVARF
                     SRTFGSLMASGLSVLEALNTSADGLNNSVFRDGLHKVASEVKAGKTIAEPLKALPIFP
                     PIVGQMVAVGEETGKLDEILLKLAGFYEREVDTVVAGITSVIEPILIVSLGGIVGFIV
                     VGVYGPLATLNNAI"
     gene            complement(2747..>4112)
                     /locus_tag="VF272_02365"
     CDS             complement(2747..>4112)
                     /locus_tag="VF272_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX7259753.1"
                     /translation="ARASDIHLEPRENLVQVRFRIDGVLRETMTLPKGIMAAVVSRIK
                     ILSNLKIDEHRVPQDGRFKFAVGSKTVALRVSTLPVMDGEKVVMRILDEGVRALTLDE
                     LGFEGIALSRIDHGIRKPHGMVLVTGPTGSGKSTTLYSVLSMLNTPGINISTVEDPVE
                     YRVPGVNQTQVNPKAGMTFAGGLRALLRQDPNVIMVGEVRDSETADLAVQAALTGHVV
                     LSTLHTNNAATTLPRLLDMQIEPFLIASTVNTVIGQRLVRRLCSICRIGYIPEGQILQ
                     DINRDFETANALKYLQDADKAKSAAPKPAPKEHHDPQAERQAKSILDKIASDPNIERS
                     ANNAKVLKQQEAELEVEQPKPALPAPDPKLRSLKDGEFALYKAGDGCDQCAGNGYMGR
                     LGIYEVLEVDEKIQQMIINHSTSEDIQKQAIESGMLTMQQDGFLKSLRGLTTIEEILR
                     VTRE"
BASE COUNT          992 a         1006 c         1021 g         1093 t
ORIGIN      
        1 tcaccggatt agcaattacg cgccggtaga gcagcttttt gacgccatcg acgtaataga
       61 ttacatcatt ggtttgcaaa ctgctatgcg aagcgtcggc ataaataagg ccaccggagc
      121 tattaactgc ggggacggcc aaaaccagga ctgagggcga cccagtattg gaactccatc
      181 cgtattggtt gcctccaggc ccattggggt ctggccactg atttgctgat tgaatggttc
      241 gagccgattt aatatcgcgc ccaaggcttt ccagggcctg cttagtatta ctttgaagga
      301 taattaacgt gtcgtttcga gcgtagtcaa tcaggcggtc aacataaaaa ccggacacta
      361 acgttgccaa tatgccaatg atggcggttg ccatcatcag ctctagcaaa gtatagccgt
      421 tttggttgtg taggttcacc ggttaatgcc tcgctgcgtc atgagggtag agatttggac
      481 attcttggta cgcttatcgt cggtgtaggt aatgataatg tcgatccgtt tcaaaccggc
      541 ttgcggtgtg gttacattgg caacggctga ttttggtgat ccgaaattgg ctggcaatgc
      601 accggtaaag gtctcagcgg gagcgccggt gggaatggca tggtagccgg cgtctcgata
      661 aatttcgagt ttttgttcgg ccaaggctgt agccacagcc atattgcgtg cttgcttgtt
      721 aagctttcca accacaatga aaatagtaat aatggccgca gacgcgacag tcacaaatac
      781 agctgttacc accagctcaa ctaaggtaaa gccgttttgt gaacggagaa ttttcaccac
      841 tgctcaccca ttactatcat tatagcgtaa gcgttttttt atagcaccta taaacctcgc
      901 tatcaaaaaa ccgcagacca acagacccgc ggttttttga ttgtgcgaaa agcttagtta
      961 gctgaattca cggtgtagac accggctgtt gcctggctgt ctttgctatt ctcgagcgta
     1021 gctcgcagtt catagctcac acaagcgccg gcggcacagc tgcctgggct cggctcgtag
     1081 gtgtagtttg cgccaccttt aggatcgttt ggaacggtct tcatgtaggg ggtgctgcct
     1141 gcggtcagaa cagtacctaa acatccgcct gcattcggaa cacaagtggc tccgcctgcc
     1201 gtgggatagc tgttgttgtc gttgtagtag gtttcaagcg cagtcttaat attgcgcaag
     1261 tcgctcttac gctgactgtc acgtgcccgc tgcgggccgc tgaccaagtt gggaatgatt
     1321 aataccgcta agatgccgat gatcacgata acgatcaaga gctctaacag tgtaaaacca
     1381 ccctgttttt ttaatgtgtt cattcgagcg ctcacctcca taaatgttgt tcttctttaa
     1441 tgataaacaa agcataagct ctattgagcc aattgtagaa tcattactcg gcttctgtca
     1501 agatttttct catggaggtt ggctttaaat agcgttattt aatgtcgcga gcggtccata
     1561 gacgccaacg acaatgaatc cgacaatacc gccaagggag acaatcagga ttggttcgat
     1621 cactgaagta atgccagcaa ccaccgtatc aacctcacgc tcgtaaaatc ctgctagttt
     1681 gagtagaatt tcatcgagtt tgccggtttc ttccccaacc gccaccattt ggccgacaat
     1741 cggcgggaag ataggcaatg cctttagtgg ctccgcaatt gtcttaccgg cttttacctc
     1801 gcttgcaact ttgtgcagtc catctcgaaa gacactgtta ttgagtccgt cagcactggt
     1861 attgagcgct tcaagtaccg aaagcccgga agccattagt gaaccgaagg tccgggaaaa
     1921 ccgggcaaca ttaactttta caataatcgg cccgaatatt ggcatacgaa tcagtatgct
     1981 gtcgaaaatg gttttgccac ggatggtatg gacgaatcga aaaccgacga ccgcaaggac
     2041 aaccaatccg attaatagga agaggccgta gttcactaag ctgtcactca tggcgagcat
     2101 cagtttactg taccaaggta attcggtacc cagactttca aagataaccg acatgcgcgg
     2161 taccaaatag gtcataagaa agacaaatgc cccaaccgta actgaaagca gtattcccgg
     2221 ataaatcata gcgctgcgaa ccttggctac caccgcggca tccttttctt gttgaacggc
     2281 taggcgctca agtacctggt ctagcaagcc gccggcttcc ccggctttaa ccatattgac
     2341 atacacttgc gagaatacat cgggatgtcc tgccaatgca ttgctgagcg tggaaccgcc
     2401 ttccacttgt ttagcggcgt cgttgataac atcacggaaa tgtttattgg tagtttggcc
     2461 gcccagcatc gacaatgcct gggtaagagg cacgccagcg ttgatcattg tggcaaattg
     2521 tcgtgaaaaa ataactttat cttcgagttt aacccggccg cccagcttta gcccgcccag
     2581 ttgcaggcct ttgcctttgg ttttagcttc ttgcacttta attaaaattg gtacaagatc
     2641 gcgttcaagc aagctagcag cagcagcggc ttggtcgttg gctacgagac tgcccttttg
     2701 tatggtgccg ctgtgatcgc gggctgtgaa attataggta gccatgctat tccctggtta
     2761 ctcgcaaaat ttcttcaatg gtagtcaacc ctcggagcga ttttaagaag ccgtcttgct
     2821 gcattgtcag catacctgac tcaattgcct gcttttgaat atcttcgctg gttgaatggt
     2881 taatgatcat ttgttgaatt ttttcgtcga cctctaaaac ctcatagatg cccaggcgcc
     2941 ccatgtagcc atttccggcg cactggtcgc agccgtcacc ggctttatat aaagcaaact
     3001 caccgtcttt gagcgaccta agcttaggat cgggagctgg cagggcaggc tttggttgct
     3061 caacttccag ctcggcttct tgttgcttca gcacctttgc attatttgcc gaacgctcaa
     3121 tgtttggatc ggaagcaatt ttatcgagaa ttgatttggc ttggcgttcc gcttgcggat
     3181 catgatgctc cttgggagcg ggcttggggg ctgcagactt agctttatcc gcgtcctgaa
     3241 gatatttgag ggcgttagca gtttcaaaat cgcgattaat atcctgcaat atctgacctt
     3301 cgggaatata gcctatgcgg caaatagagc agaggcggcg cactaagcgc tggccaatga
     3361 cggtattaac ggttgaagca atcaaaaacg gttcaatttg catatctaac aaacgaggca
     3421 gggttgttgc cgcattattg gtgtgcaggg ttgaaaggac gacgtgtccg gttaatgccg
     3481 cctgtacggc caaatcggcg gtttcgctat cgcgcacctc tcccaccatg atgacattag
     3541 gatcttgccg caaaagtgct cgtaatccgc ccgcgaaagt cataccggcc ttggggttta
     3601 cctgggtttg gttgactcct ggtacgcggt attccaccgg atcttcaacc gtggaaatgt
     3661 taatgccggg ggtattgagc atgctgagta cgctataaag tgttgttgat ttccccgatc
     3721 cggtcggtcc ggttactagc accatgccgt gcggtttgcg aataccgtgg tcgatgcggc
     3781 tgagggctat accctcaaaa cctaactcat ccaacgtaag cgctcgaacg ccttcgtcga
     3841 ggatacgcat gacaactttt tctccatcca tgaccggcaa ggttgaaacc cgcagtgcga
     3901 cggttttcga accaaccgcg aacttaaagc ggccgtcttg gggcacgcga tgctcgtcga
     3961 ttttgaggtt ggataaaatt ttaatacgtg ataccacggc tgccataatg ccttttggca
     4021 aggtcattgt ttctcgcagc acgccatcaa ttcgaaatct cacctgcact aagttttcac
     4081 gaggctcaag gtgaatatct gaagccctgg cc
//
LOCUS       DASVGB010000079        17193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_951545, whole genome shotgun sequence.
ACCESSION   DASVGB010000079 DASVGB010000000
VERSION     DASVGB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 17193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17193
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951545"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF272_02370"
     CDS             <1..303
                     /locus_tag="VF272_02370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099323.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7259754.1"
                     /translation="AREVQRVLQRYRDLQDIIAILGMDELSEEDRLTVARARKIQRFL
                     TQPFFVAEGFTGTPGVYVSRADTVRGFREILEGKHDDKPETAFLLKGGIEEVKKEK"
     gene            303..719
                     /gene="atpC"
                     /locus_tag="VF272_02375"
     CDS             303..719
                     /gene="atpC"
                     /locus_tag="VF272_02375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX7259755.1"
                     /translation="MASTIQLEVLTPGGSVFNEAVREVVIPTTGGSLGILPHHTPLAA
                     VAQAGTVDIRRKAEDRTLDHIVITRGLVEVDGERVVLMVDDAAQVGDIDEFNERQKLA
                     EAEERLKSSQEARDIASAQADILRSTTWLDASKRHK"
     gene            838..1212
                     /locus_tag="VF272_02380"
     CDS             838..1212
                     /locus_tag="VF272_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259756.1"
                     /translation="MSSIVPVQRQRYDLLLKHKSPMALRLNDVHHNQIKVGDLVDVSG
                     HNTIMDRERFKVIDRMHHPSIHHAVEHIKQSDLSARDKIQMKQAFLGVHGPEAGTRPV
                     VSLHLERHTGPSSYNRNPGSLG"
     gene            1219..2025
                     /locus_tag="VF272_02385"
     CDS             1219..2025
                     /locus_tag="VF272_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7259757.1"
                     /translation="MLAGTTTFSIPEFQAFIWDYYRQNQRKMPWRDQPTPYHVVVSEI
                     MLQQTQVARVMQKYPEFIAAFPDFHRLAAARTSEILSLWQGLGYNRRALNLHRLAALV
                     VRHYDGKLPADPAELILFPGIGSGTAGSIAVFAFNRPEVFIETNIRRVFIHHFFPDAA
                     QVSDAELRPLVEVSIDQANPREWYYGLMDYGAHIAKTVSNPNRRSRHYVQQSKFEGSD
                     RQIRGKLIRLLLEFPVLTAADIVGHFPYEPQRRVIRLLEQLTKEGFLRQQ"
     gene            2492..4246
                     /gene="dnaG"
                     /locus_tag="VF272_02390"
     CDS             2492..4246
                     /gene="dnaG"
                     /locus_tag="VF272_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7259758.1"
                     /translation="MNDVEQVKERLDVAEVIGGYIPLKQAGRNLKAPCPFHQEKSASF
                     MVSPEKGIWHCFGCNAGGDIITFVMQYEGLEFRDALALLAGKAGIELKERTPASQQAK
                     QEQKRLHEALNWAVKYYQQSLVQNPSALEYATKRRHLRRQTIQDFQIGYAPDDWNALG
                     QFLLKKKFTPIELLKAGLAGQKTGRSTIYDLYRGRLMFTICDASGAPVGFTGRVLDEE
                     QLPKYLNTPQTELYDKSRIIFGLHLAKDAIRTSNEVVIVEGNMDVVASHQAGVKQVVA
                     VSGTALTLDQLKVLGRLTKNIKICFDADAAGLRATERAIELSQKLGLTLSMVRLPEGK
                     DPDDLIQKDVQAWEKAIAEAEYVVDYLFSQIETAYDLTTATGKRQYSDRIAASLRRLA
                     DPVEQGHYIKRLTQKLDISEESVRQKINATDGQTSDLISEQRPSATIIKPSTKTARRL
                     LEESVLAINLAYTEVRSSLDDLTPAHFADADHQAILEMLQHVATASAADVAGGLPNVT
                     DYVKILALRGEEEFGSFAPAERSFEAFQLAGRLRIVTNKDTKTKLSRKLREAEDKGDA
                     DLAKRLLAQYQTLITEDD"
     gene            4256..5371
                     /gene="rpoD"
                     /locus_tag="VF272_02395"
     CDS             4256..5371
                     /gene="rpoD"
                     /locus_tag="VF272_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7259759.1"
                     /translation="MAKKAAPITEEREHNYSPAVERLLQKGKEQGFVTQQEVSQVIPD
                     AEDNLEELDELYAGLMEAGVELTDQKDRLIWEGEEDAEEGGTAKESDDYVKDIADDSV
                     RLYLREIGRVPLLTALEEVELAKRIAKGDKAAKDKMVEANLRLVVSIAKKYIGRGLDL
                     LDLIQEGSSGLLRAVEKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVET
                     INKLIRTQRRLVQELGREPLPEEIAVEMEIEVEKVNHILKIKQDIVSLDSTVGDDKDS
                     TLSEFIEDEDRPTPPEVAANQLLKEQVASVLSLLTPREQKILRMRFGLEDGKSHTLEE
                     VGLEFGVTRERIRQIEAKALMKLRRHRESKKLKDYLA"
     gene            5550..6188
                     /locus_tag="VF272_02400"
     CDS             5550..6188
                     /locus_tag="VF272_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7259760.1"
                     /translation="MSSSKDFLKKPSIIGIAGYFGAGKDKFAEMLKQHLEGRGHNVDM
                     VSSGDLIRQYVSDNKLGDPADRNVLRKTVDEVGAKHGYTYWLEQSIRQAEHGGTDILL
                     YPGLRQKVEADYIHGRGDIILAIDAPVEVRYQRAQVRGRAGDEVDFEAFKAAEEAERA
                     GTAQQIDAVIQAADTTISNDGTLEQLEQVAHAIAETYPEKPAPRYSAAGTIL"
     gene            6400..6987
                     /locus_tag="VF272_02405"
     CDS             6400..6987
                     /locus_tag="VF272_02405"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX7259761.1"
                     /translation="MMQSRTVALVAVVASLLVFIAATAAAETSSPTTQTALQATQPQL
                     TPLADSCNFRFKQSKHRSYIREVYRRDRISQKARQRIERIQRCSYSKEATRNMRADRK
                     QAAALRRQRAMRVQVPPVLHRIAQCESNGNPTAISAGGTYRGKYQFSFSTWRSVGGRG
                     DPAAASEAEQDRRALRLLRTGGLGHWPVCGPRAVR"
     gene            7068..9797
                     /gene="polA"
                     /locus_tag="VF272_02410"
     CDS             7068..9797
                     /gene="polA"
                     /locus_tag="VF272_02410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX7259762.1"
                     /translation="MAKKERLVLVDGSAVFHRGYHAIPHLSNAEGIPTNATYGFTMIM
                     LKVLADLKPKYAIVTWDKSSDTFRKELYPEYKATRKKQPDDLYAQIPYTRQVTEALGI
                     PWVELDNYEADDIIGTLGRQAKQQGLETIIVTGDLDELQLIDENTKIYTMRRGFTDTV
                     IYDLEALHDRYGVNPQQFIDLKALKGDASDNIPGVAGVGEKTAKDLIATYGSLDEVYN
                     NLDQLKGKLKERLETNKEMAYLSQKLSTIVCDAPVTLNLNTAEVGQYNKQQIHELFRR
                     MEFKALLTKLPPEMTAAAPNLFDDPMEETHSKHRSHLDTAEYKVITTEDNLTTLIKRL
                     NASPEFAFDTETTSVNEMEAELVGVSISLKEAEGFYIPVGHAKGTQLNKKVVLDAFRP
                     VFENPKIGKIAHNAKYDYKVLVRHGITPSPIIFDTMIAAFLLNPLGRSQSLDDLAYKE
                     FGIEMIPIAELIGSGRTQITFDNVPLEAATTYAAEDADITWRLYKVLAPELSKQGFDK
                     LAHATEFPLIEVLAHMEIAGIELDGAFLNKFNEKLTKDLAKLEENIWEAAGQKFNIGS
                     PAQLSDILFNQLKLSTHSVKKGKTGGYSTAAVELEKLKDAHPIIPMISDYRELSKLQS
                     TYVETLPKLVDGKGRIHTRFNQTIAQTGRLNSTDPNLQNIPVRTEIGREIRKAFVAPR
                     GKVFVSADYSQIELRIAAALSKDKGMIETFRKGIDLHQQTAAEMFGVSLNQVTPEQRY
                     GAKTINFGVLYGMSPHGLSVATGVTRDEAVQFIDRYFQIRKTLKQYIEGIKEFAHKHE
                     YTETLLGRRRPSPEINSNNFQIRNAAERIAVNVPLQGTAADIIKLAMIALVPKLPVGA
                     QLLLQIHDELIVEANEKQAEAVAGIMKDTMENIYDLGVPIAVDTKIGHSWGDL"
     gene            9957..10340
                     /locus_tag="VF272_02415"
     CDS             9957..10340
                     /locus_tag="VF272_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259763.1"
                     /translation="MPVDDLKDRYRGLEHLFGLIEGRLTSPLKRGATIAGRAPTASVV
                     TDLIEELTTGFTPNDWGRVAAKLKLETGPAAPPLAIQVGLERAFYRENPRIAAQLENN
                     RHRRLDEAIPTPPHVLETLDLSVAA"
     gene            complement(10508..10987)
                     /locus_tag="VF272_02420"
     CDS             complement(10508..10987)
                     /locus_tag="VF272_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259764.1"
                     /translation="MRIATDPKERMMQLEATVNKIGENLREMKLITDAYRFTPSAESA
                     SLEHKRQRRVTLEVHGGQRRQLRFELKRLVWNLAALKAPVPASSDADHRKTVPCPFCA
                     GSIKRHAVNCMHRQPNAYEDLAAGLLAAKAGSPLPEDASINCQRGFAIGLELGDMVD"
     gene            11085..11963
                     /gene="mutM"
                     /locus_tag="VF272_02425"
     CDS             11085..11963
                     /gene="mutM"
                     /locus_tag="VF272_02425"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX7259765.1"
                     /translation="MPELPEVETIRIGLSQTLIGLKITDIEVLHPKSFQSSSELTDLQ
                     AIGATITKLDRRGKVLIIELNTGYSLLVHLKMTGQMVLIRPEGGRYAGGHPTKSMADE
                     LPDKSTKVIFRLSDGSILYFNDQRVFGWIKLVSKAEVLRDSLIARLGPEPLTEAFELA
                     PFAAVLVRRGGSPIKAVILDQSTVAGVGNIYADESLHLAKIHPARLAGSLAKPEIKRL
                     YEAIKTIIALGVKRGGTSFTHYVNALGGKGDYLHSARVFRRQGLTCPVCESIIIKTRV
                     AGRGTHVCPSCQRAPQ"
     gene            11960..12895
                     /gene="holA"
                     /locus_tag="VF272_02430"
     CDS             11960..12895
                     /gene="holA"
                     /locus_tag="VF272_02430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7259766.1"
                     /translation="MIFFFYGPNSYEARQQIGKLARDYEQKSGSNFGLERIDGAKAKP
                     DELQAALQASPFLANSRLVIIEDLGLNKALAPKINDFIEDIPSTTIGVFYDPAVDQRT
                     TYFKTLSSKARTVKFEPLLPAKLQIWVSRQAKAAGAEIERPALMRILELAGEDQWRLS
                     NEISKLAAYRSPITLEAVNEQVEEGSSETIFNLVEAMTAGKTTQAMQGYQKLRQEGQN
                     EIYMLSMVIWQLRNLLIAKTAGTVTSAQLAKAASMSPYVAGKMLAKRHLFAEESLKQA
                     FLEAVDTEYKIKSGAGAADILVEQLIYRIAGRIKN"
     gene            complement(12892..13113)
                     /locus_tag="VF272_02435"
     CDS             complement(12892..13113)
                     /locus_tag="VF272_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259767.1"
                     /translation="MTEHRAKRTPGEEAQAKLLDACEQLMAHVKLLRDYATTPDNIWL
                     ARGELNKINEMGTEIDALLNEIKAGGDSS"
     gene            complement(13145..13531)
                     /gene="rpsT"
                     /locus_tag="VF272_02440"
     CDS             complement(13145..13531)
                     /gene="rpsT"
                     /locus_tag="VF272_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7259768.1"
                     /translation="MPIIKSAIKRVKQSAKRRSHNLQVKRAIHQDMRAFTDALATGDT
                     KTTAETLKAAISEIDRAVKKGTLHRNTAGRRKSRLQKQANAIGAKPTAKVKTKSAVKA
                     RATTTIKATSAAKKPATTKAKSKKAA"
     gene            13644..15047
                     /locus_tag="VF272_02445"
     CDS             13644..15047
                     /locus_tag="VF272_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX7259769.1"
                     /translation="MDKELTPKQQTSEAIRQADTILLMTGQHPNIDQVASTIALAAIL
                     RKFGKKVTTVISDDIPAASRFLPTHLIERDLGGMRDFIVQVDLSNAEVDKLKYAIAEG
                     KLNINITPFSGGFSQRDVTFTYGDLHFDLVIVLGVLSYSRIDKVYVQNAEMMRHIPLI
                     NIDFHRINEQYGAVNLLETSAASLAEILIALAESLQSGLIDADIATTMLTALYAATDR
                     FTAVHTTAKTMTVAAQMVAGGADHQKVVKHLFGKGERGEKRSTPPSSAPLSKSADQAS
                     PAASAPRQTARIELEPVDPRPTIEKPALRLQRLINPEPGVSMSIRSESAAQAAPADSP
                     KPETPKAVEPAPVAAPEPAPTPAAVADPTPQTQPKSELPLDDFKPAAEPQPEPSLKIQ
                     SGMEDLLPPQHIELTPPSDGSATSPMRTNPLLGNTGPEPLINAPNEPPKAQSGPAKAA
                     NPTNNPIFAQRLDEFEY"
     gene            14944..15753
                     /gene="truB"
                     /locus_tag="VF272_02450"
     CDS             14944..15753
                     /gene="truB"
                     /locus_tag="VF272_02450"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7259770.1"
                     /translation="MLPTNRLKPNPVRLRPLTLPIIRFLLNDSTNLSIKTGESSLDPS
                     GLLLVDKPSGISSFDIIRILRRQTAVRKIGHAGTLDPAATGLMLMLFNKATKQAGQLS
                     GLNKRYEAEITLGATSTTGDREGEIIATDHRRPALSEVAAALHVFTGSVTQTPSVYSA
                     IKVEGVRAYKLARAGKKVIMPSREVTIHSIKLVSYSFPIIGIEAHVSSGTYIRSLAAD
                     VGTYLGTGGYLSRLRRIQVGAFTIASAYQLGEVQLSTLKKQLVTASDLTKS"
     gene            15886..16170
                     /locus_tag="VF272_02455"
     CDS             15886..16170
                     /locus_tag="VF272_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259771.1"
                     /translation="MALTATDLSNIKVSIADVVQPRFEELNLRFDETNSRLSQFRADF
                     DNFAAATQRKFHTAFSDIAFTREDLQAVKEMVTDHGFRLARLEQKDGRRE"
     gene            16225..16491
                     /gene="rpsO"
                     /locus_tag="VF272_02460"
     CDS             16225..16491
                     /gene="rpsO"
                     /locus_tag="VF272_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7259772.1"
                     /translation="MLKNDIKQKVITDHQSHKKDTGSPEVQIGIFTKQIQELTAHLQT
                     HKKDEHSRRGLLKMVGKRRRLLDYLSKNDADRYRLIVKKLDLRR"
     gene            16682..>17193
                     /locus_tag="VF272_02465"
     CDS             16682..>17193
                     /locus_tag="VF272_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7259773.1"
                     /translation="MQQFETEFCGRPLTIETGKIGFLADGAVTVRYGDTMVLGTAVMS
                     NQPREGIDFFPLLIDYEERMYASGKISGSRFVKREGRASEAATLTSRLIDRPIRPLFP
                     KGFRNDVQGVATVLSADLVYSPDIIAMIAISSALTISGAPFEGPIAGVRVGRIDGKLV
                     VYPTSAEMTV"
BASE COUNT         4348 a         4296 c         4437 g         4112 t
ORIGIN      
        1 gctcgcgagg ttcagcgcgt attgcagcgg taccgtgacc tgcaagacat cattgcgatt
       61 cttgggatgg atgaactcag tgaggaggat cgtttaactg ttgcccgtgc ccgtaagatt
      121 cagcgatttc ttacccagcc attcttcgta gccgaaggtt ttaccggtac ccccggcgtc
      181 tatgtcagtc gtgccgacac cgtgcgcggt ttccgtgaga ttctcgaagg caagcatgac
      241 gacaagcccg aaactgcctt ccttctcaag ggtggcattg aagaagtcaa aaaggaaaag
      301 tagtggcaag caccatacag cttgaggtgc taactcccgg cggatcagtt tttaacgagg
      361 ctgtccgcga ggtggttatt cctaccactg gcggttccct aggcattttg cctcatcaca
      421 cgccattggc tgcggttgct caggcgggaa ccgtggatat ccgccgtaag gctgaagacc
      481 gaaccttaga tcatattgta attacccgag gattggtaga agttgatggt gaacgcgttg
      541 tgttaatggt ggatgatgcc gctcaagtag gggacatcga tgagtttaat gagcgccaga
      601 agctagccga ggctgaagaa cgtttgaaat cgtcccaaga agctcgcgat attgcctcag
      661 cccaagccga cattctgcgt tctaccactt ggcttgatgc cagcaagcga cataaataac
      721 cagtcgctta aatacgttct tccctgccta gcgcctatcg ttcgatcgca tgtcagaatg
      781 tatagcggac acgagaagaa gtatttgtgc cgctaaaatt agcgctataa taaaagcatg
      841 tcctcaattg tcccagttca acgccagcgg tatgatcttc tcttgaagca taagagcccg
      901 atggccctgc gcctgaatga tgtacatcac aatcagatta aagtcggcga tctcgtagat
      961 gtcagcggtc acaatactat tatggatcgc gagcgcttta aggtgattga tcggatgcat
     1021 caccccagta ttcatcatgc cgttgagcat attaagcagt ccgacttaag cgcccgtgat
     1081 aagattcaaa tgaagcaggc ctttctcggt gtgcatggcc ccgaggctgg cacccgtccg
     1141 gtggtttcat tgcacttaga acgccacacc ggtccatcat cctacaatcg aaaccccggc
     1201 agtttgggat agtccggttt gttagccggc acgacaacgt tttcgatacc cgagttccaa
     1261 gcatttattt gggattatta ccgccaaaat cagcgtaaaa tgccgtggcg cgatcagcct
     1321 acgccatatc atgtggtggt ttcagagatc atgttgcagc aaacgcaagt agcccgggtc
     1381 atgcaaaaat accctgagtt tattgctgct tttccagatt ttcatcgcct ggcggctgcc
     1441 cgtacttccg agatactcag cctgtggcaa ggattgggct ataaccgccg ggctttaaat
     1501 ttgcaccggt tggctgcgct ggttgttcgg cactatgacg gtaagcttcc ggccgatcca
     1561 gctgaactca ttttgtttcc aggtattggt tcaggcaccg ctggctcaat cgcagtgttt
     1621 gcgtttaacc ggccggaggt gttcattgaa accaatatcc gtagagtctt tattcatcat
     1681 ttctttcctg acgccgctca agtgagcgat gccgagctgc gcccgctggt tgaagtgtcg
     1741 attgaccagg ctaatcctcg cgagtggtac tacggattaa tggattacgg cgctcatata
     1801 gcgaaaactg tttcaaaccc caaccgccgc agccgtcatt atgtccagca gtctaagttt
     1861 gaaggctcag atcgccaaat cagaggtaag cttattcgct tgttgcttga atttccggtg
     1921 ctaacggcgg ctgatattgt ggggcacttt ccgtatgaac cgcagcgtcg tgtcatcagg
     1981 ctgttggaac aactgacaaa agaaggcttt cttcggcaac aataatttgc ttttcaaatg
     2041 ctattcaagc ataaggatta gaggtataaa tgagtacttg caccgcgtgt gcaggcagag
     2101 cctgaatgct ttatggcgtt tatggctaga acgacaacga cgtacgagag gagctgcatg
     2161 gcaagtacat caaccccccc tcaaggagga aggcggttgg cgtcatcgac gctcgaagtc
     2221 tcaacgcttc cttcccgaac tgctgctgtc cgtcgccggg ctcagcaggc agcaatcgca
     2281 aatgccatca gggcaatgcg agccagcggg aaaaaaagcc cgggtgggca tacatcggct
     2341 ggtccttcag cgggccctta gcttcaggtc tggccaccac atcctctcgc aatcggccgt
     2401 ctgcctcctc tgggggcgga cggtctagcg acacaaccat acagtttcat gtcatgttcg
     2461 tattttgttc gtttactggt ataatacact catgaacgac gtggagcagg tcaaagaacg
     2521 tcttgatgtg gcggaagtta ttggcggcta tattccattg aagcaagccg ggcgcaacct
     2581 gaaagcgccg tgtccgtttc atcaggaaaa atccgctagc tttatggtga gtccggaaaa
     2641 agggatttgg cactgcttcg ggtgtaacgc cggcggtgat attattacct ttgttatgca
     2701 atatgaagga ttggagtttc gcgacgctct ggcgttactc gccggtaaag ctggcattga
     2761 gttgaaagag cgtacaccag ccagccagca agccaagcag gagcaaaagc gtttgcatga
     2821 agccctaaac tgggcggtta agtattatca gcaaagtctg gttcaaaatc cttcggcctt
     2881 ggagtacgct accaagcgcc gccatctgcg ccgtcaaacc attcaagatt ttcaaattgg
     2941 ctacgcccca gacgactgga atgctcttgg tcagtttctc ctgaagaaaa aatttactcc
     3001 gatcgaatta ctcaaagctg gtttggccgg gcagaaaacc ggtcgttcca caatatatga
     3061 tctctatcgc ggccgcctga tgtttactat ctgcgatgcg agcggtgcac cagtcggctt
     3121 taccgggagg gttctcgatg aggagcagct gcctaagtat ctcaacacac ctcaaacgga
     3181 actatatgat aaaagtcgaa tcatatttgg actgcatttg gccaaggacg ctattcgcac
     3241 ttccaatgaa gtggttattg tcgaaggcaa tatggatgtt gtggccagcc accaagccgg
     3301 ggttaagcaa gtggtagcgg ttagcgggac agcacttact ttagatcagc tcaaagtgct
     3361 tggacgcctc acgaagaata ttaaaatatg cttcgatgcc gatgccgcgg ggctgcgggc
     3421 aaccgagcgg gctattgagc tcagccaaaa attaggccta acactttcca tggtccggct
     3481 tccggaaggg aaggatcctg atgatctgat ccaaaaagat gttcaggcat gggaaaaggc
     3541 gatagccgag gccgagtatg tagttgatta tttgttcagc caaattgaaa cagcctacga
     3601 cctaactact gccaccggca agcgccagta tagcgaccgc atcgccgcca gcttgcgccg
     3661 cctggctgac ccggtagagc agggtcatta cataaagcgc ttaacccaaa aattagatat
     3721 tagcgaagag tcggttcggc aaaaaattaa tgctacggat ggccaaacca gcgatcttat
     3781 cagtgaacag cggccttcag ccaccatcat caaaccctca actaaaactg ccaggcgatt
     3841 gcttgaagag tcggtactgg ctattaacct ggcttatacg gaagtacgca gcagtttgga
     3901 tgacctaaca cctgcccatt ttgccgatgc tgaccatcaa gcaattttgg agatgctgca
     3961 gcacgttgca accgcttcgg ctgctgacgt tgctggcgga ttgccaaacg ttacagacta
     4021 tgtcaaaata cttgcactac ggggcgagga agaatttggt tccttcgctc ccgcagaacg
     4081 cagttttgaa gcatttcaac tggcaggacg tctgcgaatt gtaacgaaca aagataccaa
     4141 gaccaaactc agccgtaagc ttcgcgaggc tgaggataaa ggtgatgccg atttggcaaa
     4201 acgcctgtta gcgcagtatc aaacacttat aactgaagac gactaaggag gttttttggc
     4261 caaaaaagca gcaccaatca ctgaagagcg ggagcataac tattctccgg cagttgaacg
     4321 gttactacaa aaaggaaaag aacaaggttt tgttacccag caagaggtat cgcaagttat
     4381 tccggatgcc gaggataatc ttgaagagct cgatgagttg tatgctggtc taatggaagc
     4441 tggtgttgag ctaaccgatc agaaagaccg gttaatttgg gaaggcgagg aagatgccga
     4501 agaaggggga accgccaaag aaagcgacga ctatgttaaa gacattgccg atgactcggt
     4561 acgcttatat ctgcgtgaaa ttggacgcgt tccgctctta actgcgctgg aagaggttga
     4621 gctagctaaa cgaattgcta aaggcgacaa agctgccaag gataaaatgg tagaagccaa
     4681 tctgcggctg gtggtttcga ttgctaaaaa atatattggc cgcgggctgg acttgctcga
     4741 cctgattcaa gaaggctcca gcggactgct tcgcgcggtt gagaaatttg attacaccaa
     4801 aggctttaaa ttctcaacct atgctacatg gtggatccgc caagctatta cccgtgctat
     4861 cgctgaccag gcccgcacta tccgtatccc agtgcacatg gttgaaacaa ttaataagtt
     4921 aattcgcacc cagcgccgtc tggttcagga gcttggccgc gagcctctgc cggaagagat
     4981 tgccgtggaa atggaaatcg aggttgaaaa ggtcaaccat atactcaaga ttaagcagga
     5041 cattgtttcg cttgattcca cggttggcga tgataaggac tcgactctga gtgaatttat
     5101 tgaagatgaa gaccggccca cccctccgga agtagccgcc aatcagttgc ttaaagagca
     5161 ggtggccagc gtgctgagcc ttctaactcc tcgggagcag aagatcttgc gcatgcgttt
     5221 cgggttggag gacggcaaaa gccatacgct tgaagaggtg ggactggagt ttggagttac
     5281 tcgcgagcgt atccggcaga ttgaggccaa agctctcatg aagttgcgcc gccatcggga
     5341 aagcaaaaag ctgaaagact acctcgctta agcttacgtt tatcgccaaa taatgacaag
     5401 aagcaacgga aagccgttgc ttctttattt ttgtcttggt gccggtttag gcacaaaact
     5461 ggcatagata gtacaccaaa catacacaat tttggcacaa atccggcatt acgctcggtc
     5521 aaccttaata catctgctag aattgttgga tgagctcatc aaaagatttc cttaaaaaac
     5581 catctatcat cggcatagcc ggctactttg gcgctggcaa agataaattt gccgaaatgc
     5641 tgaagcagca tttagagggc cgcggccaca atgtggatat ggtatcttcg ggcgacctca
     5701 ttcgccaata tgtcagcgat aacaagctag gcgatccggc cgaccgcaat gtcttgcgca
     5761 aaactgtcga tgaagttggc gccaaacacg ggtataccta ttggctcgag cagtccatca
     5821 ggcaggccga acacggggga accgatatcc tgttgtatcc gggattgcgc cagaaggtag
     5881 aagccgacta tattcatggt cggggcgata tcatcctggc tattgatgct ccggtggaag
     5941 tccggtacca gcgggcgcaa gtccgaggac gtgccggcga tgaagtcgat tttgaggcct
     6001 ttaaggcagc cgaagaagcc gagcgggccg gtaccgccca gcaaattgat gctgtcatcc
     6061 aggctgccga tacgacaatc agtaatgatg gaaccctgga acagcttgag caggttgcac
     6121 atgccattgc cgaaacctac cccgaaaagc cagcgccacg ttatagcgct gccggaacta
     6181 ttttgtaatc ttgcatatgc ttggctcttt ccgccaggct tgctactatc tatgggtatc
     6241 atccagcgcg ttaaccgctc tggagtaatc gtctagaatc gtacggaggc cgcagcgcgg
     6301 actcccgaga tggaaattct cgcgaagggc gattgctcat tcccaaaccc acctgacgaa
     6361 gtaaggcgta ctcgtcccag ctcacacagg aggcgtaaga tgatgcaatc acgtacagtt
     6421 gccctagtgg cagttgttgc ttcgctgttg gtctttatcg ctgcaacggc agcggcagag
     6481 actagcagtc ccacaactca gacagcgctg caagcgaccc aaccgcagtt gactccgctg
     6541 gccgattcgt gcaacttccg gttcaagcaa tcgaagcatc gcagctacat ccgcgaggtc
     6601 taccgccgcg accgtatctc tcaaaaggcg cggcaacgga ttgagcgaat acagcggtgc
     6661 tcgtactcaa aggaggctac tcgtaacatg cgggctgatc gcaagcaagc ggccgccctg
     6721 cgccggcaac gcgccatgcg tgttcaggtt ccgccggttc tacatcgaat cgcgcagtgc
     6781 gaaagcaacg gtaaccctac ggctatctca gccggaggta cctaccgtgg caagtaccag
     6841 ttcagcttct ctacctggcg atcggtcgga ggcaggggtg acccggccgc cgcatcggaa
     6901 gctgaacagg atcgccgggc actccggttg cttcggacag gaggccttgg ccattggccg
     6961 gtttgcggtc cgcgggcggt tcgttagtaa gcgctctcgc tccggtggcc tcagggtagc
     7021 attccctgag gccaccgtgc ccacttgatc tgcgttatac tagctacatg gctaaaaaag
     7081 aacgtcttgt cctggtggat ggcagcgcgg tatttcaccg cggttatcat gcaattccac
     7141 atctttccaa tgctgaaggt attcctacca acgctaccta tggcttcacc atgataatgc
     7201 tcaaagtatt ggccgatctc aaacccaaat atgcaattgt tacatgggat aaatccagcg
     7261 atacgtttcg caaggagctg tatccggaat ataaggctac ccgtaaaaag cagccggatg
     7321 atctctatgc ccaaattcct tatacccgcc aagttactga agcattaggg attccttggg
     7381 tcgaacttga taactacgaa gccgacgaca ttattggcac gcttggccgt caggctaaac
     7441 agcaagggct tgaaaccatc attgttaccg gtgatttgga cgagcttcaa ttgattgatg
     7501 agaacaccaa aatatacacg atgcgccgcg gctttactga caccgtaatt tatgacttgg
     7561 aggcgcttca tgaccgttac ggcgtgaacc cgcagcagtt tatcgacctc aaagcattga
     7621 agggagatgc cagcgataac attcccggcg tcgctggggt gggggagaaa accgccaagg
     7681 atctcattgc cacgtatggc tcgctcgacg aagtgtataa caatttagat cagctaaaag
     7741 gcaagctcaa ggagcgattg gaaacgaaca aggagatggc ctatttgagt caaaaattgt
     7801 ctacaatcgt ttgcgatgcg ccagttacac ttaacctcaa tacagcggag gtcggccagt
     7861 ataataagca gcaaattcat gaactgtttc gacgcatgga gtttaaagcg ctgcttacta
     7921 agcttccacc ggagatgaca gcagcggctc ccaatttgtt cgatgatcct atggaagaga
     7981 cgcactccaa gcaccgctct catctagata cggctgagta taaggtgatt acgactgaag
     8041 acaatttaac aacacttatc aagcgcttaa atgctagccc agagtttgcc ttcgataccg
     8101 aaacaaccag cgtaaatgaa atggaagccg agctagtagg ggtctcaatc agcctcaaag
     8161 aagccgaagg attttatatt ccggttggtc atgcgaaggg gacacagctg aacaaaaaag
     8221 ttgttcttga cgcatttagg ccagtatttg aaaaccctaa aattggtaaa atagcccaca
     8281 atgcaaagta cgattataaa gttcttgttc ggcatggcat tacgccgagc cccattattt
     8341 tcgacacaat gatcgccgct tttttgctta acccacttgg caggtcgcaa tcgttggacg
     8401 atttggccta caaggaattt ggcattgaaa tgattccgat tgctgagctc attggtagcg
     8461 ggcgaaccca aattaccttt gacaatgttc cgcttgaagc tgctacaacg tatgctgccg
     8521 aagatgccga tataacgtgg cggctttata aggtcttagc gccggagctt agtaagcagg
     8581 gctttgataa gcttgcccat gccactgagt ttcctctcat cgaagtactg gctcacatgg
     8641 aaatagcggg cattgagctg gatggggctt ttctcaacaa atttaacgag aagttaacaa
     8701 aagacttagc caaactagag gaaaacattt gggaggctgc cgggcaaaaa tttaacattg
     8761 gttcgccggc acaattatct gacatcttat ttaatcaatt gaagctttca actcatagtg
     8821 tcaagaaagg caaaaccggc ggctactcaa ccgccgccgt tgagctggaa aagctgaaag
     8881 acgcccaccc gattattccc atgatttccg attaccgcga gctatccaag cttcagtcaa
     8941 catacgtcga aaccttgccg aagctggttg acggcaaagg ccgcatccat acccgcttca
     9001 accaaaccat tgctcaaacc gggcggctta attcaacgga ccccaatttg cagaatattc
     9061 cggtgaggac cgagattggc cgtgagatcc gtaaggcctt tgtggctcca agggggaaag
     9121 tgtttgtaag tgccgactat tcgcaaattg aactgcgaat tgctgcggcc ttaagtaaag
     9181 ataaagggat gattgaaacg tttcgaaagg ggatcgacct tcatcaacaa accgccgccg
     9241 aaatgtttgg tgtatcactc aatcaagtga ctccggagca acgctacggc gcaaaaacca
     9301 ttaacttcgg tgtcctctat ggtatgagtc ctcatgggtt gagcgttgct accggggtaa
     9361 cgcgggatga agcggtacag ttcattgacc ggtattttca aattcgtaaa acgctcaagc
     9421 aatacattga gggaataaaa gagtttgccc ataagcatga gtacaccgaa accttactgg
     9481 gccgccgccg tccttcgccg gaaatcaact ccaacaactt tcaaattcgt aacgctgccg
     9541 agcgcattgc ggtaaatgtg ccgctgcagg ggactgccgc cgacattatc aagctagcta
     9601 tgattgcact cgtaccgaaa ctgcctgttg gtgctcagct cttgctgcaa attcatgacg
     9661 aactgatcgt ggaagctaat gaaaagcagg ctgaagcggt tgccggaatt atgaaagaca
     9721 cgatggaaaa catttacgac cttggtgttc cgattgccgt tgatacaaaa atcgggcata
     9781 gctggggtga cttgtaagta atcataaaaa taacggctct gacgtgcttt ttactgtgac
     9841 taccactagc ataactattg ctcccatttg gaaataaagt tataacagaa ttatggtgga
     9901 caggtagttg cagacgctgt atcgacatgt acgtcacaac aacaggaagg cacaacatgc
     9961 ccgtcgacga cctcaaggac aggtaccgcg gactcgagca cctgttcggc ctcattgagg
    10021 gcaggcttac gagcccgctc aagaggggcg caaccattgc tgggcgcgcg cccacggcct
    10081 cggtcgtgac cgatctcatc gaggagctca cgaccggctt cacgcccaac gactggggcc
    10141 gagtcgccgc caagctcaag ctggagaccg ggccggcagc accgccgctc gccatccagg
    10201 tcgggctgga acgggcgttc tatcgggaga atcccaggat tgccgcacag ctcgagaaca
    10261 accgccatcg gcggctggac gaggcgattc ccacccctcc gcatgtgctc gagaccctcg
    10321 atctttccgt tgcggcctga tcgccggctc atcccctcct accacatctg gtccggattc
    10381 ccgcaagggc gtccgggccg ggaggcgcac ttattcaatg tacataaaac tattagtgcg
    10441 tgtagcccgt aggcttcact tgttaggtga aacctacggg ggcgccgaac gatggatggg
    10501 tagtccatca atcgaccatg tcgcccagct ccagcccgat ggcgaacccg cgctggcagt
    10561 tgatactggc atcttccgga agtggacttc ctgccttggc ggccagaagc ccggcagcca
    10621 ggtcttcgta tgcgttcggc tgtctgtgca tgcagttcac ggcatgtctc ttgatcgaac
    10681 cagcacagaa cgggcagggg acggtcttgc ggtgatcagc atcggaagat gcaggaaccg
    10741 gagccttcag cgctgccagg ttccagacca gccgcttgag ttcgaaacga agctgacggc
    10801 gttgaccgcc atgcacttcg agggtgaccc ttctttgacg cttgtgctcc agcgaggcgc
    10861 tctcggcaga cggagtgaac ctgtaggcgt cggtgatcaa cttcatttct cgcaggtttt
    10921 ctccgatctt gttgaccgtt gcttcaagct gcatcatccg ctcttttgga tcggtggcga
    10981 ttcgcatatc tgcctccctt ccttggtcga tgtgcgggat acactaacct agcattttta
    11041 cagggtaatt tcaagtcgtt tcaggagtgc gctacaatac ctctatgccc gaattacctg
    11101 aagtggaaac aatccgcata ggattaagcc aaaccttgat cggcttgaag attaccgata
    11161 ttgaagtgct gcatcctaaa tcctttcaat cgtcgtctga attgaccgat ttgcaggcga
    11221 ttggtgccac tattaccaag ttggatcgtc gcggcaaggt cttaattatt gaactcaata
    11281 ctggctatag tctgttggtg catcttaaaa tgaccgggca gatggtacta ataaggccgg
    11341 aaggggggcg ctatgccggc ggtcatccca ccaagagcat ggccgatgag ctgcccgata
    11401 agagcacaaa ggtaattttt agactcagcg atggttcaat tttatacttc aatgatcagc
    11461 gggtattcgg ctggataaaa ttagtatcca aggccgaagt actgcgtgat agtttgattg
    11521 cccgactcgg cccggagccg ctaaccgaag ctttcgaatt ggcgcctttt gcggcggttc
    11581 tagtccgacg tggcggatcg ccgattaaag ccgttattct tgaccagtcg acggtggcgg
    11641 gggtcggtaa tatttatgcc gatgaatcgc tgcatctggc taaaatccac cccgctcgtt
    11701 tggccggaag cctcgctaag ccagaaataa aacgcctgta tgaagctata aaaaccataa
    11761 ttgcgcttgg cgtgaagcgg gggggtacca gctttactca ttatgtgaat gccctcggcg
    11821 gaaaggggga ttacttacac agcgcccggg ttttccgccg ccaagggcta acctgcccgg
    11881 tatgtgaaag cattattatt aaaacgcgcg tagccggaag gggaactcat gtttgtcctt
    11941 cgtgtcagag ggcgccgcaa tgatcttctt tttttatggg cccaatagct atgaggcacg
    12001 tcagcaaata ggcaagctgg cccgcgatta tgagcaaaaa tctggcagta attttgggct
    12061 tgaacgtatc gatggggcaa aggccaagcc ggatgaactg caagccgctt tacaggcaag
    12121 tccctttttg gccaatagcc gcttggtgat tattgaggat ctcggtctta acaaggcgct
    12181 tgcacctaaa atcaacgact tcattgagga tattccctca accacgattg gcgtatttta
    12241 tgaccctgcc gtcgatcagc gaaccaccta ttttaaaacc ctgagcagta aagcccggac
    12301 cgtaaagttt gaaccgttac taccagccaa actgcaaatc tgggtttcgc gccaagccaa
    12361 ggcagccggt gccgagattg aacgccccgc cctaatgagg attctggagc tggcaggaga
    12421 ggatcaatgg aggttaagca atgaaattag caagctggcc gcatatcgtt caccgattac
    12481 gcttgaagcg gtcaatgaac aggttgaaga ggggagcagc gagacaatct ttaatttggt
    12541 cgaagccatg acggccggta aaactacgca ggctatgcag gggtaccaaa agctgcgtca
    12601 ggaaggccaa aatgaaattt atatgctgag catggttatt tggcagttgc gcaatctctt
    12661 aatcgcaaaa accgccggaa ccgtgacatc ggcccagttg gctaaggccg ccagtatgtc
    12721 accgtatgta gcgggcaaaa tgctggctaa gcgccactta tttgctgaag agtcgttaaa
    12781 gcaggccttc cttgaggcgg ttgatactga atataagatc aaatctggag ccggagctgc
    12841 cgatatcttg gtagagcagc taatttatcg cattgctggg cgaatcaaga attaggagga
    12901 atctccgccg gccttaatct cattgagcag cgcatcaatc tcggtgccca tttcatttat
    12961 tttattaagc tcgccccggg caagccaaat attatccggc gtcgtggcgt aatcgcgcaa
    13021 tagctttacg tgagccatta attgttcgca ggcatcaagt agttttgctt gggcttcttc
    13081 gccgggcgta cgtttggctc ggtgttcggt catactactt cctcagtggc ggttgggcct
    13141 actattacgc ggcttttttt gacttagctt tagtggttgc cggtttttta gcagcggatg
    13201 tagccttgat tgtagtggtt gccctagcct taacggcaga cttggttttg actttagcgg
    13261 taggcttggc gccaatggcg ttagcttgct tttgcaagcg ggacttgcgc cgtccggcag
    13321 tattgcggtg aagggttcct ttttttacgg cgcgatcaat ctcggaaata gcggccttta
    13381 aggtctcagc tgtagttttt gtatcaccgg tagccagggc atcggtaaaa gcgcgcatgt
    13441 cctgatggat tgctcgttta acttggagat tatggcttcg gcgcttggcc gactgcttta
    13501 ctcgtttaat tgctgatttg attattggca taacagatga gactatagcg gaaaaggagc
    13561 gggctgtaaa gtagcgaaac acctcagcta ttgacagata ggacatttct tgatacaata
    13621 ataaaatctg tagagcaaga acgatggata aagaattaac tcccaaacaa cagacttctg
    13681 aagcgattcg ccaggctgat accatcctgt tgatgaccgg gcagcaccca aatatcgacc
    13741 aggttgcatc aaccattgcc ctggcggcta ttttgcgtaa gtttggtaaa aaagtcacta
    13801 ccgttatcag tgatgatatt cctgcggctt cccgtttctt gccaacccat ctcattgagc
    13861 gcgacttagg cggcatgcgt gatttcatcg tgcaggtcga cctttccaat gccgaggtag
    13921 ataaactgaa gtatgcaatt gccgaaggca agctcaacat caatataacg ccattctccg
    13981 gtggctttag ccaacgcgac gttaccttta cctacggcga ccttcatttt gacttggtaa
    14041 ttgtgctggg cgtactttca tatagccgca tcgataaggt atacgtccaa aatgccgaga
    14101 tgatgcgcca tattccactg atcaatattg attttcaccg cattaatgaa cagtacggag
    14161 ctgtaaatct gttggaaacc tcggcagcca gtctagcgga gattttaatt gccctggccg
    14221 aatcgcttca atcggggtta attgatgctg acattgccac caccatgctc accgcgctgt
    14281 atgctgcaac cgaccgcttt actgcggttc atacaaccgc taaaaccatg acggtagctg
    14341 cccaaatggt agctggcggt gccgaccatc aaaaagtggt taagcactta tttggaaaag
    14401 gggagcgagg cgaaaagcgg tctacgccgc cttcttctgc accattgtct aaatcggctg
    14461 atcaggcttc tcccgccgca tctgcgccgc gccaaactgc caggattgag ttggagccgg
    14521 ttgatccgcg tcccaccatt gaaaagcccg ctttacgcct tcagcgactg attaaccctg
    14581 aacctggcgt aagtatgtct atccgttctg aatcggccgc tcaggctgct cctgcggatt
    14641 ctccaaagcc ggaaactcct aaagccgtcg agccagcgcc ggttgcagct cctgaaccgg
    14701 cgccaacccc tgccgctgtt gcggatccga ctcctcagac tcaaccgaaa tctgaattgc
    14761 cgcttgatga tttcaagccg gctgcggaac ctcagcctga accctcatta aaaattcaat
    14821 ccggcatgga agatttgctt ccgcctcagc acattgagct gacgccgccc tcggatggta
    14881 gcgcaacgtc accgatgaga accaacccct tgcttggcaa taccggtccg gaacccttaa
    14941 ttaatgctcc caacgaaccg cctaaagccc aatccggtcc ggctaaggcc gctaacccta
    15001 ccaataatcc gatttttgct caacgactcg acgaatttga gtattaaaac cggggaaagt
    15061 tcgctagatc ccagcggcct tctattagta gataagccct ctgggattag ttcattcgat
    15121 attattagaa tcttgcgtcg ccaaaccgcg gttcgcaaaa ttggtcacgc cggaacgctg
    15181 gatccggcag ctaccggttt gatgcttatg ctctttaata aggccactaa gcaagctggc
    15241 cagctgtccg gtttaaataa gcgctacgag gctgagataa ccttgggagc aacctcgaca
    15301 actggcgacc gggaagggga gataatcgca acggatcatc gccgccctgc gcttagcgaa
    15361 gtcgctgctg ccctgcatgt ctttaccggc tccgttactc aaaccccttc agtctattca
    15421 gccataaagg ttgagggtgt aagggcatac aagctggccc gggccggtaa aaaagtcatc
    15481 atgccgtcgc gcgaggtaac cattcactct ataaagcttg tatcgtattc ctttcccatc
    15541 attggaattg aagcccacgt atcgagcgga acctacatcc gttccctggc tgcagatgtt
    15601 ggtacgtatc tcggtaccgg cggatatttg agtcgattgc ggcgtataca ggtgggcgcc
    15661 tttacgattg catctgctta tcagcttggc gaagttcagc tatccacgct caagaaacaa
    15721 ctcgtaaccg catccgattt aaccaagagt taatagaacc aaggtataat aaaagcattt
    15781 taaacctctt caacaccaag aaacagactt gtttgttcgg atagtgttcg ctagcataga
    15841 ggaagcagga catgccacta ccatttacgc aaaaaggagc ttgttatggc cttaacggca
    15901 accgatctta gcaacattaa agtcagtatt gccgatgttg tccagccgcg attcgaggag
    15961 cttaatctac gcttcgatga aaccaatagc cgccttagcc aatttagggc tgatttcgac
    16021 aactttgcgg ccgccaccca gcgcaagttt catacggcat ttagcgatat tgcctttact
    16081 cgagaagacc ttcaggcggt aaaggagatg gtaaccgatc atggttttcg tttggctcgg
    16141 ctagaacaga aggatggccg aagggagtaa ttactgcttc ggcatattgc cctttctcct
    16201 gtaatttggt accattactg cgcaatgcta aaaaatgata taaagcaaaa ggtcattacc
    16261 gaccatcaat cgcacaaaaa ggacaccggt tcgccggagg ttcaaatcgg catatttacc
    16321 aagcaaattc aggagctcac cgcgcatctt caaacccata aaaaggatga acactcacga
    16381 cgcgggttgt taaagatggt gggcaagcgc cgtcggctgt tggactactt aagcaagaat
    16441 gatgctgatc gctaccggtt aattgtaaag aagctcgatc tgcgccgata gtttggttgc
    16501 ggcgaggcgc tttaagggaa aatgaagcgt caggctgcgc ccaaagtggg cgaggtacat
    16561 aattaagcgc atacgtgaca gagctttgat tccgatgata atcggattct tatccttgga
    16621 acgtatgtga gaaatggaga ctatggcaca tcaagagatt atccacccgt ttggtggaaa
    16681 ggtgcagcag tttgagaccg aattttgtgg ccgtccacta actattgaaa ccgggaagat
    16741 cggatttttg gccgatggtg ccgttacggt tcgttacggc gataccatgg tgctgggaac
    16801 cgctgtaatg tctaaccagc cgcgcgaggg gattgatttc ttcccgctgc tgattgatta
    16861 cgaggagcga atgtatgctt cgggcaaaat ttccggcagc cgttttgtta agcgtgaggg
    16921 ccgggcttcc gaagctgcca cgttaacctc ccgtttgatt gaccgtccga tccggccact
    16981 atttcctaaa ggcttccgca atgatgtcca aggagtagca acagtactca gtgccgacct
    17041 tgtgtatagc ccggatatta tcgccatgat tgccatctcc agtgctctta ccatctccgg
    17101 cgccccattc gaaggtccga ttgccggggt tcgcgttggt cggattgacg gcaaactagt
    17161 ggtgtatcca acttcagccg aaatgaccgt tag
//
LOCUS       DASVGB010000080         3214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_576641, whole genome shotgun sequence.
ACCESSION   DASVGB010000080 DASVGB010000000
VERSION     DASVGB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576641"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VF272_02470"
     CDS             <1..335
                     /locus_tag="VF272_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7259774.1"
                     /translation="AGEMKHGANALIDKDLLMIFLALKNELYAKTMSNLQEVRARAGT
                     VIAVGTEGDTAIKKQADDVLYVPDVSPWIQPLVANIPLQLLAYYIAVERGTDVDQPRN
                     LAKSVTVE"
     gene            complement(397..864)
                     /locus_tag="VF272_02475"
     CDS             complement(397..864)
                     /locus_tag="VF272_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259775.1"
                     /translation="MLLVKVSLFFPCLFLLGVWVWHGVAPVFMLGWPLASLLAIYVIE
                     RLEDSKNKWWDGVLGGLKGAMMFGMLGVFSFVMGLSLYGSVMAYPKLFDYPSKADIIE
                     MSPLADSLDRVAVLGACWFGAALGVIAVHTGRAFCRRRHQHGNDSQPLQQSPA"
     gene            999..1619
                     /gene="lexA"
                     /locus_tag="VF272_02480"
     CDS             999..1619
                     /gene="lexA"
                     /locus_tag="VF272_02480"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX7259776.1"
                     /translation="MAEPLTKRQKEVLDYITQYIEVNGYAPSYREIAEAFHLGSVATV
                     ADHVETLVTKGLLKKGDNSARSLQLVQEEDFHDDHFVGLPILGMIAAGQPIQTMTGHA
                     ETLEVPQFMVGSKNSYVLQVKGDSMIEEGIFEGDYVVIQEKEVPSNGDMVVALVNNEE
                     ATLKRYYKEKGHTRLQPSNDAYDPIIVPASTPLKIQGVCIGVIRKY"
     gene            2220..>3214
                     /locus_tag="VF272_02485"
     CDS             2220..>3214
                     /locus_tag="VF272_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7259777.1"
                     /translation="MASPFESLFSGSESPFDLLAEDFPQFASQMGYLLPRDREATNIE
                     EFISDHTKELIQKAAETAVAFGRDEVDTEHLLYALVDSDIASEIFRQVKIDPKELKNY
                     IDKNAPKGEQREGRQELTVSPRVKSVLDRAFGIAQEMGHSYIGPEHLLVSLASEEEGL
                     AGDMLRKYGLTPEAIRQQTIKVVGRGAKEGRVESQSTTPQLDKYSRDLSQLAKQGKLD
                     PVIGRAGEIENTIEILSRRTKNNPALIGEPGVGKTAIVEGLAQRIQNGKVPQVLQDKR
                     VLEVNLNSIVAGSKYRGEFEERLKAVLDEIIEHQDELIIFIDELHTIVGTGQGGA"
BASE COUNT          861 a          764 c          863 g          726 t
ORIGIN      
        1 cggccggcga gatgaagcat ggcgccaacg ccttaattga taaggacctg ctaatgatat
       61 ttttggcatt gaagaatgag ctatatgcca aaaccatgag caatctgcag gaagtccgcg
      121 cgcgggccgg caccgtcatt gctgttggaa ccgaagggga cacggccatc aaaaagcagg
      181 ccgatgatgt actgtacgta cctgacgtgt cgccatggat ccagccgctg gtggccaata
      241 tcccgttgca gctgctcgct tattatattg cagtagaacg ggggaccgac gtggatcagc
      301 ctcgaaatct ggcgaaaagc gtgacggtgg aataataatt gctgtctggc gacagggaca
      361 ccgatgaggc atccccgccg cctaaactcc ggtgcgctat gccggcgatt gctgtagcgg
      421 ttggctgtcg ttgccatgct ggtggcgccg gcgacagaac gctcttcccg tgtggactgc
      481 tatgacgccg agggcggcac cgaaccagca ggcacccagc acagctacgc ggtcaagcga
      541 atccgccaac ggcgacattt cgatgatgtc ggccttgctc ggataatcga acagcttggg
      601 gtaggccatg acgctgccgt agagcgagag gcccatcacg aaggaaaaga cgcccagcat
      661 tccgaacatc atcgcgccct tcagcccacc gagtacaccg tcccaccact tgttcttcga
      721 gtcctcgagg cgctcgatga cgtagatggc aagcagtgaa gcgagaggcc agcccagcat
      781 gaataccggg gccactccat gccagaccca cacccccaat aggaaaaggc aggggaaaaa
      841 aagcgaaact ttcaccagca gcatgcggtc acatccttag tagactgcgg acgaaaaata
      901 ttatgtctta aaagttttat tttgtaaagg caaacgcctc ttgacagttc ggttttcgtt
      961 cgccatacta aaaccaaaca ctagcataag gaggttgcat ggcggaacca ctcacaaaac
     1021 ggcaaaaaga ggtactggac tacatcaccc agtacatcga agttaatggg tatgcgccaa
     1081 gttaccgcga gattgctgaa gcatttcatt taggctcggt tgcaacggtg gccgaccacg
     1141 ttgaaacctt agtgacaaaa ggcctgctta aaaagggcga caactcggcc cgctccttgc
     1201 agctggttca agaagaggat tttcatgacg accattttgt ggggcttccg attcttggca
     1261 tgattgctgc tgggcagcca attcaaacca tgaccggtca tgccgaaacc ttagaggtgc
     1321 cacagtttat ggtggggagc aagaactcct atgtgctgca agttaagggt gactcaatga
     1381 ttgaagaagg tatttttgaa ggcgactacg tggttattca agaaaaagag gttccttcaa
     1441 acggcgatat ggtagtcgcg ctggttaaca acgaagaggc caccctaaag cgttattaca
     1501 aggaaaaagg gcatacccgt ctgcagccat caaatgatgc gtatgacccg attatcgtgc
     1561 cagcttccac cccattgaaa attcaaggag tatgcattgg ggtcatccgc aaatactaac
     1621 aatcaaacta ctcagcctgc tgtaaataag cgtcagcttc gtcgaatgtt ccggcagtat
     1681 cttttctctt atacgtttga accgctgtct cggcctaaag agaacgcggc taagccaaaa
     1741 gatcagttga ccaggtagag ccgggaatcg tatactgtcg aagtacttac gcgatccggc
     1801 gtagctcagt ggtagagcag ttgactgtta atcaattggt cgcaggttcg aatcctgccg
     1861 ccggtgccat tgcactaatg cttaaacatc agcttactgt tgccttttta agttcgtcca
     1921 tcatcacgga gccaaaatac ttcaggcccc tgtgaacatg tctatagatt tacagacttg
     1981 aacttgctta cataagggtg gacagttgca gggtcgccag cgcccgcttt tgagttcccg
     2041 cagatctgtc cgcagcagca ataatttaag ttgattgggg tatgtatatg gctgaaggaa
     2101 tatgtgacat ttgcaagagg cggccagcca ctcaccgagt tcgggttagc caaaatggac
     2161 gcgaatccga gatggaatta tgtaatatcg attacgctag gttgcgacag caacagtcga
     2221 tggcctcacc atttgaatca ctgttttccg gatcggaaag cccctttgat ttattagctg
     2281 aggattttcc tcaatttgcc tcccaaatgg gctatctgct gccccgtgat cgtgaagcga
     2341 ccaacattga ggagtttatc tctgaccata ccaaagagtt aatccagaaa gccgccgaaa
     2401 ctgcagtagc ctttggccga gatgaggtcg ataccgagca tctgttgtat gcgcttgtgg
     2461 acagtgatat agccagtgag atttttcgcc aggtaaaaat cgacccaaaa gagttaaaga
     2521 actatatcga taagaatgcg cccaaaggag agcagcgtga gggtcggcaa gagcttaccg
     2581 tttcacctcg ggttaagagc gtgcttgatc gagcttttgg tatcgcccaa gagatgggcc
     2641 acagctatat cggcccggag cacctgcttg tgagcctggc ttcagaagag gaaggactag
     2701 ccggtgatat gctacgaaag tatggattga cacctgaggc gattcgccag cagactataa
     2761 aagtggttgg tcgaggagct aaagagggac gagttgagag tcaatcgacg accccgcagc
     2821 ttgataaata ttcccgtgat ctttctcagt tggcgaagca aggcaaactg gatccagtga
     2881 ttgggcgggc cggcgagatt gaaaatacta ttgagatcct ttcccgacgc actaagaaca
     2941 accctgcttt aattggtgag ccaggggttg gcaagactgc aatcgtggaa gggttggctc
     3001 agcgcataca aaacggtaaa gttccgcaag ttttgcagga taaacgggta ctagaggtta
     3061 acttgaactc aatagtggcc ggttcaaagt accgtggcga atttgaagag cgcctaaagg
     3121 cagtcctgga tgagatcatt gagcatcagg atgaattgat tatctttatt gatgaactgc
     3181 atacgatagt agggacgggt cagggaggag ccga
//
LOCUS       DASVGB010000081         7212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_25107, whole genome shotgun sequence.
ACCESSION   DASVGB010000081 DASVGB010000000
VERSION     DASVGB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25107"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1127)
                     /locus_tag="VF272_02490"
     CDS             complement(36..1127)
                     /locus_tag="VF272_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7259778.1"
                     /translation="MTKLITSLYDPAKTYEDNFIQGPFGDPVEPYRNEGEPLYEAFGH
                     KLYSPFGIPAGPLLNSNYVKDAFERGFDAVVYKTQRSVPFAVNTFPQILYVDVDGDLT
                     PEKAAKPLLGQTSTSRPVEEFSITNSFGMPSKGPDYWVEDMKKALSYQGKGQLMIMGI
                     VGTIQKGFSQEDYLKDFAYTAGLAVDGGAKVIEANLSCPNVATEGILCYSPDSVIDIV
                     QGVKERIGNTPLIIKIGDYAPEQQELLEQIVQEAVPYIAGISSINTIPAPIVDENGNQ
                     ALPGPNRLKSGVCGASIKWAGLDMSRKLKAIREKYAMEYKIIGVGGVMSAADFQEYRD
                     AGADIVQSATGAMWNPSLAAEVKATLSRQ"
     gene            1167..1985
                     /gene="pyrF"
                     /locus_tag="VF272_02495"
     CDS             1167..1985
                     /gene="pyrF"
                     /locus_tag="VF272_02495"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7259779.1"
                     /translation="MEFKTKLSSAVQQNDTLLCVGLDPVRDNLPDHLKNHGNAFFTFN
                     KAIIDATADLICAFKPNSAFYEAEGADGISQLHDTCRYITDNHPHIPIILDFKRADIG
                     STNQAYSKFAFDYLGVDAITVHPYLGRDAIQPFLDYKDKGIMILVRTSNPGSGEFQNL
                     EVDGVKLYLKVAKAVQDSWNANQNCQLVIGATYPEELAEIRKLVGDDMIFLVPGIGAQ
                     GGQTDLLRGGFTKNGDGLIISSSRETIYASGGENFADAARAKATELRDQINSYR"
     gene            complement(1982..2659)
                     /locus_tag="VF272_02500"
     CDS             complement(1982..2659)
                     /locus_tag="VF272_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259780.1"
                     /translation="MSELMTEAEALQALKDKGSLYAPEDGYHFKGTSGKHINGYCNID
                     PVLPFPNLLSRMTEAIVANFKNDGVQTVIVPATGGIPLSQWGPYHLENMTGGEVLGVW
                     ADKAKPTGFILGRAGFPLAVKGRKVLVLEDIINTMYSIKQVVDLVREVGGEVVGVGSV
                     IANRGVSAEALGVEKFFNLATFSYDAWEEPDCELCAKQVPMVIDVGHGDEFADEHPEY
                     PTRRVLT"
     gene            2859..3977
                     /locus_tag="VF272_02505"
     CDS             2859..3977
                     /locus_tag="VF272_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7259781.1"
                     /translation="MNTKPSKHLSNKLTTRLAELVHAHTLSTQPDINKVELERIASNL
                     KTAGMRCTIGENGGYPHLYAYFQPADQTEVWLSAHLDVVEGSEDQFNLKKADGKLFGR
                     GVYDMKFAIPVYEAVAQHFSKAKPAMGIIITTDEEVSGLYGSSHLAKKLDLKGKTIIL
                     PDGGGPWMIERSAKGALWVHLEAQGTPAHAARPQQGVNAIELVMKAVAEIRYALAAGS
                     GHQPSDTTVNLGKVVGGEAINKVPARAHADIDIRFAPPLSLEDVKATIVRTESTHPEV
                     IITFGPEAQALSANIVHPDTKLLQSIMCQCVGKEVEYADSLGGSEARYYAAAGAHPIV
                     FYPGGGGLHTDSEYIYEESLEQCYDCIRQFVEARCARP"
     gene            complement(3974..6055)
                     /gene="fusA"
                     /locus_tag="VF272_02510"
     CDS             complement(3974..6055)
                     /gene="fusA"
                     /locus_tag="VF272_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7259782.1"
                     /translation="MAREYSLEKTRNIGIIAHIDAGKTTTTEGILYRTGKTHKIGEVH
                     QGEATMDWMEQERERGITITSAATTCFWDDTRINIIDTPGHIDFTVEVERSLRVLDGA
                     VTVFDGKMGVEPQSETVWRQADKYGVPRICFINKINQTGGDFYKSLDSIHERLSKRAY
                     PIHLPIGFEQTINGIIDLVAMKAYTYKDYTDKELTETDVPDDMKDKAAEYRQELIEAA
                     VEADDVLFEKYLEGTELTVDEIKGAIRKSVLSGTFYVVSGGDGRGVIVEKLLDAVVDY
                     LPSPLDVPPLVGVDPKTGEDIVRKPSDDEPFSALAFKIAADPFVGKLAFFRVYSGSLE
                     SGSYVLNSSTGQKERIGRILRMHANSREEVQKVYAGEIAAAVGLKGTTTGHTLSDEKQ
                     PIILESITFPEPVIQIAIEPKTKADQEKMGIALQRLAEEDPTFRISSNHETGQTLIAG
                     MGELHLDIIVDRMKREFKVEANVGRPQVAYRETIKKTAKGQGKFVRQSGGRGQYGDVW
                     LELTPQEPGTGYEFDNAIVGGVIPREYIKPVDEGIKEAMQNGVLAGYPVVDVKAKLYD
                     GSYHDVDSSEMAFKIAGSIAFQEVAKKADPIILEPIMKVEVVVPEEYMGDIIGDLNSK
                     RGRIEKMDDRGSAKVVDAFVPLSEMFGYTTTLRSMTQGRGSNTMEFYHYAEVPPNVAQ
                     EIIGKQTAAKA"
     gene            complement(6200..>7212)
                     /locus_tag="VF272_02515"
     CDS             complement(6200..>7212)
                     /locus_tag="VF272_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7259783.1"
                     /translation="NDGSHTISAVARDVAGNTSDLASISSSTFTVANLDTTPPNPPTS
                     LSGATTQPTSSTAAKVTLSWPAASDVITNASGVKGYYVYRGTMVISPLVAGTSYTDIN
                     VPTGSLSYTVRSVDNAGLTSTTTSPAYATTINVPVDTTAPTIPTNVTATAVSSSQINL
                     VWSPSSDTGGSGLRGYNIYRNGSTTPHNTIPLTATSYGDASGLSPSTTYSYTVEAVDN
                     AGNKSPKTVAVSVSTAGTTSADVTPPSVAWVVPAPGDTVRGTLNEDNGNCRATATDSS
                     GIAKVEFYLDGRLTNTEVSDPYSCIIDVRRYSRGWHTIGVQAFDNAGNVSPRVSEEVR
                     FR"
BASE COUNT         1800 a         1862 c         1690 g         1860 t
ORIGIN      
        1 tttttatgct gtgctagtcg ccggagcagc aagtattatt gccggcttaa cgtcgcttta
       61 acttcggccg ctaagctagg attccacatc gcgccggtgg ccgactgcac aatgtcggca
      121 ccggcatccc gatattcctg aaagtcagcc gccgacatca caccgccgac accaataatc
      181 ttgtattcca tagcatattt ttcgcggatt gcttttaatt tgcggctcat atccagtccg
      241 gcccacttaa tgctggcacc gcacaccccg ctctttagcc ggtttggtcc aggcagcgcc
      301 tgattgccat tttcatccac gatcggtgcc ggaatagtat ttatcgagga aattccggca
      361 atatagggta ccgcctcttg cacaatttgc tccaatagct cctgttgctc tggagcataa
      421 tcccctattt tgatgattaa gggtgtatta ccaatccgct ccttaacacc ctgaacaata
      481 tcgatcaccg agtctgggct atagcaaagg ataccttcgg ttgcgacgtt aggacatgac
      541 aggttggctt caattacctt tgcgccgcca tcaaccgcta atccagccgt ataagcaaaa
      601 tcctttagat agtcttcttg gctgaaccct ttttgaattg tacccacaat ccccatgatc
      661 ataagctgac ctttcccctg gtagctcaac gctttcttca tgtcctcgac ccaataatct
      721 ggtcctttcg agggcatccc aaacgaattg gttatcgaaa actcttcaac cggccttgag
      781 gttgaagttt gaccgagcag cggcttggca gccttttccg gcgttaggtc accgtcgaca
      841 tcaacatata agatctgcgg gaatgtatta actgcaaatg gcacactgcg ctgagttttg
      901 tagacaaccg cgtcaaaacc gcgctcaaag gcatctttta catagttgct attaagcaac
      961 gggcctgccg gaataccaaa tggtgaatag agcttatgtc caaacgcttc atatagcggt
     1021 tccccttcat ttcggtaagg ctctaccggg tcgccaaacg ggccttgaat aaaattgtct
     1081 tcgtacgtct tagccgggtc atacagcgag gtgatgagct tggtcaatct tttgctccta
     1141 tctgctatct atactaaagt aaagctatgg aatttaaaac caagctgagc tctgcggtgc
     1201 agcagaatga cacattgctg tgcgtcggtc tcgaccctgt ccgcgacaac cttcccgatc
     1261 atttaaaaaa tcatggaaat gcttttttta cttttaataa agccattatt gatgctacgg
     1321 ccgatcttat ttgcgctttt aagcccaact ccgcgtttta tgaagctgag ggcgctgacg
     1381 gcatcagtca gctgcatgac acctgccggt acatcaccga caaccaccca cacataccaa
     1441 ttatccttga cttcaagcgc gctgacattg gcagcaccaa ccaagcctat tccaagtttg
     1501 cgtttgatta tctgggggtg gacgccatta ccgttcaccc ctatcttggc cgcgacgcca
     1561 ttcaaccctt cctggactat aaagacaagg gaataatgat tctggttcga acctctaacc
     1621 cgggatcggg agagtttcaa aacttagaag tagacggcgt gaagctgtat cttaaagttg
     1681 ctaaagccgt ccaagatagc tggaatgcta atcagaattg ccagctggta attggtgcca
     1741 cttatccgga agaattggct gagattcgca agctggttgg cgatgacatg attttcctgg
     1801 tacctggtat tggagctcaa ggaggtcaga ccgatctgtt acgtggcgga ttcaccaaaa
     1861 acggcgacgg acttattatc agttcatctc gcgaaaccat ttatgcctca ggcggcgaga
     1921 attttgcaga tgctgcacgc gccaaagcca ccgaactgcg cgaccaaatt aactcatatc
     1981 gctaagttaa tactcgacgc gttggatact cggggtgctc atccgcaaat tcatcgccat
     2041 gaccaacatc gattaccatc gggacctgct tggcacaaag ctcacaatct ggttcctccc
     2101 aagcatcata ggaaaaagta gcaagattga aaaacttttc aacaccgagg gcttcagcac
     2161 tgacgccacg attggcaatc accgacccca cgcccaccac ttcaccaccc acctctctga
     2221 caagatcaac aacttgtttt attgaataca tcgtattgat aatatcttcc agcaccagca
     2281 ccttgcggcc cttaacagca aggggaaatc cagcccgacc aagaatgaaa cccgtgggct
     2341 tagctttatc ggcccataca ccgagcacct ctccgccggt catgttttca agatggtaag
     2401 ggccccactg ggataaaggg atcccccctg tcgccggcac aatcaccgtt tgaacgccat
     2461 cattcttgaa attggcaacg atcgcttcgg tcatccggga gagcaggttg ggaaaaggta
     2521 gaaccgggtc aatgttacag taaccattga tatgtttgcc actggttccc ttaaaatggt
     2581 agccatcttc gggagcatac aatgagccct tatcttttaa agcctggagg gcctcagctt
     2641 cggtcattaa ttcgctcatg attgctcctt ttgaatgtac tctaccacct cgtttggagt
     2701 ttcggcaaaa cgtagggctt gtttaccagg taagtgcttt ttcagctgtt ttctcttatt
     2761 gcacattttt aacattatga gacaaaggtg cgttaagaaa aacggccaga aaaacaatca
     2821 ttttccgggc gatgcatcaa tgcgctataa tcaagaaaat gaatactaaa ccatcgaaac
     2881 atctttcaaa caagttaacc acccggctgg cggaattggt gcatgcccat acactatcca
     2941 cccagcccga tattaataag gtagagttgg aaagaatcgc ttcgaatctg aagacggctg
     3001 ggatgcgctg taccattggt gaaaatggtg gctaccctca cttatacgcg tactttcaac
     3061 ctgccgacca aacagaagtc tggttaagcg cccatcttga tgttgttgaa ggtagcgaag
     3121 atcagttcaa ccttaaaaaa gccgatggca agttgtttgg ccggggtgtc tatgatatga
     3181 aatttgctat tcccgtttac gaagccgtgg cccagcattt tagtaaagct aaacctgcta
     3241 tgggcatcat tattactacc gacgaagagg ttagcgggct ctatggtagt agccacttgg
     3301 caaaaaaact ggatctgaaa ggaaagacga ttattctgcc tgatggcggc ggtccatgga
     3361 tgatcgaacg ttcagctaag ggcgcgttat gggtgcatct tgaagctcaa ggtactccag
     3421 cccacgccgc ccggccgcaa caaggagtga atgcgattga gctggtcatg aaggcggtag
     3481 cagagattcg ctatgcactc gctgctggca gcggacacca gccttccgat acaaccgtca
     3541 atctaggtaa agttgtcggc ggagaagcca taaataaagt gccggcccga gcacatgctg
     3601 atattgatat ccgatttgcg cccccgctat cactggaaga cgttaaggcc acgatcgtac
     3661 gaaccgaaag cacacatccc gaggtcatta ttacctttgg tccagaggcg caagcgcttt
     3721 ctgctaatat tgtgcacccg gatacgaagc tcttacaaag cattatgtgc cagtgtgttg
     3781 gtaaggaagt cgaatatgcc gactcgttag gcggttcgga agcccgctat tacgccgcgg
     3841 ccggagctca ccctattgtt ttttatccgg gcggcggcgg gcttcacacc gacagcgaat
     3901 acatttatga agaaagtttg gagcagtgct atgactgcat tcgccagttt gtagaagctc
     3961 gctgcgcccg gccttaagct ttagcggcag tttgcttgcc aataatttcc tgggcaacgt
     4021 tcggcggcac ttcggcgtag tggtagaact ccatggtatt ggagcctcgc ccttgcgtca
     4081 ttgaacgcaa tgtggtggta tagccaaaca tttccgaaag cgggacaaac gcatccacca
     4141 ccttagcgct tccccggtca tccatttttt caatccgacc gcgcttggag ttaagatcac
     4201 caataatatc gcccatatac tcttccggca ctaccacttc aactttcata attggctcta
     4261 agataattgg gtcggctttt ttagcaacct cttggaaggc aatcgagcca gcaattttaa
     4321 aggccatttc actggagtcg acgtcatgat acgaaccgtc gtacagctta gccttcacgt
     4381 caacgaccgg ataaccggcc agtacaccat tttgcattgc ctctttaatg ccttcatcca
     4441 ctggtttaat gtattcgcgg ggaatgacac cgccgacgat ggcgttatca aattcatagc
     4501 cggtgccggg ctcctggggg gtcagctcca gccagacatc gccatactgg ccacgcccgc
     4561 cggactggcg cacaaacttg ccctgtcctt tggcggtctt tttgatggtt tcacgatacg
     4621 ccacttgcgg ccggccaaca ttggcttcca ccttaaactc acgcttcatc cggtcaacaa
     4681 taatgtctag gtgcagctcg cccataccgg cgattaaggt ttggccggtt tcgtgattgc
     4741 tggaaatccg gaaggtcgga tcttcttcgg ctaaacgctg caaggctatg cccatttttt
     4801 cttggtcggc tttggttttc ggttcgatgg caatttgaat gaccggctcg gggaaggtaa
     4861 tcgactccaa gataatcggt tgcttttcgt cgctcaaggt atgaccggtt gtcgtacctt
     4921 tcaggccaac agctgcagca atttcgccgg cgtacacctt ttgaacttcc tcacggctgt
     4981 tagcatgcat tcgcagtatg cggccaatac gctccttttg gccggtactg gaatttagga
     5041 catatgatcc cgattcaagg ctaccggaat aaacccggaa gaaagccagt ttaccaacaa
     5101 acggatcggc ggctattttg aaagctagag ctgaaaatgg ttcatcgtcg cttggtttgc
     5161 gcacaatgtc ttcaccggtt ttgggatcta cgccaaccaa cggaggaaca tcgagcggac
     5221 ttggcaaata atcgactact gcatcaagca gtttttcaac aataaccccg cgaccatcac
     5281 cgcctgacac cacataaaag gtaccggaaa gcacgctttt acggatggcg cctttgattt
     5341 cgtcgacggt cagttcggtg ccttcaaggt atttttcaaa caaaacatca tcagcttcaa
     5401 cagcggcctc aatcagctct tgccggtact ccgctgcctt atctttcatg tcgtcaggga
     5461 catcggtttc ggttaattct ttatcggtgt agtccttgta ggtgtaggcc ttcatcgcaa
     5521 ccaaatcaat aatgccgtta atcgtttgtt caaaaccaat cggcagatga attggatagg
     5581 cccgcttgct taaacgttcg tgaatgctgt ccaggctctt ataaaaatca ccgccggtct
     5641 gattaatttt gttgatgaag caaatccgcg gcacaccata cttatcggcc tgacgccata
     5701 ccgtttcact ctgtggttcc acgcccatct tcccatcgaa aacggtaact gcgccatcta
     5761 aaacacgcaa ggagcgttct acttctacgg taaaatcgat gtggccgggg gtatcaataa
     5821 tgttaatccg ggtatcatcc caaaaacagg ttgtggcggc cgaagtaatg gtaattccgc
     5881 gctcgcgctc ctgctccatc cagtccatgg tggcctcgcc ctgatggact tcgccgatct
     5941 tgtgagtctt gccggttcga tataaaattc cttcggtcgt ggtggtcttc ccggcatcaa
     6001 tatgagcaat aatgccaatg ttacgggtct tctctaagct atattcgcga gccataaact
     6061 acactaattc ctttccacta ttgtcggcta aaattaagac aaaataaaaa gcgtcggcta
     6121 gacgcctgct tcacgttaat tgtactaaca atacgaacag attacaatac cgcccaaaac
     6181 cgctatcaac tgctcaaagc tatctaaatc gtacctcctc actaactctt ggacttacgt
     6241 tcccagcatt gtcgaaggcc tggactccaa ttgtatgcca accacgagaa tatcgtctga
     6301 catcaataat gcaggaataa ggatcactta cctcagtatt ggtaagtcta ccatcaagat
     6361 aaaattcaac ttttgcaata ccggaggaat cggtagcggt agccctgcaa ttgccattgt
     6421 cctcattaag agtgccccta accgtgtccc caggcgcagg aaccacccag gcaacgcttg
     6481 gaggtgttac gtctgcagat gttgttcccg ctgtactcac actcactgcg acggtcttag
     6541 ggcttttgtt tccagcgtta tccacagcct cgacggtata gctgtaggta gtgctaggac
     6601 tcaaaccact agcatccccg tagctggtgg cggtaagcgg gattgtgtta tggggagttg
     6661 tagacccatt acgatagatg ttgtagcctc gcagtccgct gccgccggta tcggaactgg
     6721 gagaccatac aaggttgatt tgcgaggaag aaacagcggt agcggttacg ttggtgggaa
     6781 ttgtgggggc agtagtgtca acaggaacgt ttatggtggt ggcataagcc ggagaagtcg
     6841 tggtgctggt aagtccggcg ttatcaactg aacgtaccgt gtaactaaga gagcctgtcg
     6901 gtacgttgat gtcggtatag ctagtccctg ccaccaaagg actaattacc atggtacccc
     6961 ggtaaacata gtagccttta acacccgacg cattagtaat aacatcactg gcagctggcc
     7021 aactaagggt tacttttgct gccgtagaag atgtaggctg ggtagtagca ccgctcaagc
     7081 ttgttggtgg attgggtggc gttgtgtcaa ggttggcaac cgtaaaggta ctgctgctga
     7141 tactagctaa atcggaggtg ttgcctgcta cgtcgcgagc cactgcacta atggtatgac
     7201 tgccgtcatt ca
//
LOCUS       DASVGB010000082         5668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_61625, whole genome shotgun sequence.
ACCESSION   DASVGB010000082 DASVGB010000000
VERSION     DASVGB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61625"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /gene="ligA"
                     /locus_tag="VF272_02520"
     CDS             complement(<1..1591)
                     /gene="ligA"
                     /locus_tag="VF272_02520"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7259784.1"
                     /translation="MNRTEASQRIDKLREQINDYRYHYHVLDTSTMPEAAADSLKHEL
                     SQLEQEFPDLITPDSPTQRVAGKPSAQFTPAPHQIPMLSLNDLFDRSEVEAWLARTSK
                     QAPEFDGNVYAEIKMDGLAASLIYEDGIFIQGLTRGDGRLGEDVTANLRTINTVPLRL
                     RAAADVPAEVYRGRFEVRGEVLMYKEVFQRLNTERERDGKPLFANPRNTAAGTIRQLD
                     PKLVDERQLSFHVYGIATPPDSVDTHEREHLLAAQLGFRLDPNAQTVQGVEGIMQYTA
                     EWEEKRKYLPYGTDGVVLTVNTSSVYRALGVVGKAPRGSAAYKFPAEQATTKLKNIMV
                     SLGRTGAATPFAVLEPVLIAGSTVQMATLHNAGEVARKDIRVGDTVVVQKAGDIIPEV
                     VQSLPKLRTGNETAFVMPTRCPVCNQILFKEPHEAIWRCINFDCPALERGRIIHFASK
                     DAFDIEGVGEKSVDSLLDAKVIQDAADLFSVSVDDVLTLERFADISAHKLVASIQSRK
                     RVSFDRFIYALGIRHVGQQTAR"
     gene            complement(1588..2346)
                     /locus_tag="VF272_02525"
     CDS             complement(1588..2346)
                     /locus_tag="VF272_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259785.1"
                     /translation="MAKAQESPWRLFKQALRDYRQHWTLLVGVVLVVSLPVALLTTFK
                     IVETSSDTTAAAYVSFAQLIMNTAIIYAIIKVIKGEELPNVRTIYYQSSQAFLRLFLA
                     SVVLVLTLLFFLLGLFIVAAGVIAPNAQLGSAELGLLVGLAIIIALPSLWLLPKSMWA
                     LYLVFESEIGPLEAVRASWRLTAGRTWKTLGYLAALTLFLLGLMAVPLIGFLFLQNLT
                     QWPIWEPLLQLTATLLVVPISNLYLYRYLRNMRR"
     gene            complement(2347..3234)
                     /locus_tag="VF272_02530"
     CDS             complement(2347..3234)
                     /locus_tag="VF272_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011102296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7259786.1"
                     /translation="MYSQIASNKRKSILLVTAFVILISVLGYSYSLATNQPGIFLGVS
                     IFALVYALISYRFSASIALAIAGARPVAKKDAPELYRLVENLSITAGLPMPKVYIIDD
                     PSPNAFATGRNPKNAVVAVTSGLLQLLDKEELEGVLAHEMSHVGNYDIRFMAIVMVLV
                     TVISLVSNFFLHMSWGGGGRREGGNPILAIVAIVASLLAPLIAGLLQLAVSRKREYLA
                     DASGALLTRYPEGLARALEKISAASEQQPLAHASTANAHMYFSNPLPSVGSFMASLFS
                     THPPAAERIKRLREMGDKV"
     gene            complement(3238..3759)
                     /locus_tag="VF272_02535"
     CDS             complement(3238..3759)
                     /locus_tag="VF272_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259787.1"
                     /translation="MDDITKAYSWKDSLELSKQLVQLCERFSDVDTNVLVWHLRQAVV
                     DIPATVAADLQANRPANLDPSIKLMAELELVHKIYPGIDTGSTEELLRNLLERMRSDR
                     FGERESEHEDHHSEESDEHSEHEEHHEDHHQNHRETHHHIPEVASLKVDSVGSQPVIS
                     IDPTQPSSRHNEG"
     gene            complement(3778..4332)
                     /locus_tag="VF272_02540"
     CDS             complement(3778..4332)
                     /locus_tag="VF272_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7259788.1"
                     /translation="MELWIVLGVIAVIAIALIVIYNGLVSSKNRVDESWSDINVQLKR
                     RYDLIPNLVTSVKGYATHESKVFQDVTTARSAAMGATSVHEKAQAENMLAETLKSLFA
                     VSEAYPDLKASQNFQQLSEELVDTEDKIQAARRFYNGNVRDFNTKLQVFPTNMVGGMM
                     GFKDREFFEADNQTEIEKPVAVQF"
     gene            complement(4412..4498)
                     /locus_tag="VF272_02545"
     tRNA            complement(4412..4498)
                     /locus_tag="VF272_02545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4463..4465),aa:Leu,seq:taa)
     gene            complement(4499..4572)
                     /locus_tag="VF272_02550"
     tRNA            complement(4499..4572)
                     /locus_tag="VF272_02550"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4538..4540),aa:Cys,seq:gca)
     gene            4700..5152
                     /locus_tag="VF272_02555"
     CDS             4700..5152
                     /locus_tag="VF272_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259789.1"
                     /translation="MSQKRFERVTGAAIGAIAWFALTYPEATTNHNMIDIDHPFWKFT
                     FPERGYVIILEVDRDDNPTNLLCRTRFHQPGGRNRHSLPLRDPIREVYVFTDAQMVFS
                     EEHGLSVRQLQEIVQRTMRQAVEAAEAAVADVSGEAIPDAAVSVRGTE"
     gene            5279..>5668
                     /locus_tag="VF272_02560"
     CDS             5279..>5668
                     /locus_tag="VF272_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259790.1"
                     /translation="MKLHRKLNRIATARRFNIKMLIVFIILASVGGVYVVYRSNAATY
                     GHNPYMYATAVDVRTGATVPNVDIRVRSTDDQRYCITPYTPTDGGGHTYFWSCQLGGV
                     DGAGRVFDHYYVYYAAAPPGYQIADHVG"
BASE COUNT         1441 a         1522 c         1346 g         1359 t
ORIGIN      
        1 cacgggcggt ttgctggccc acgtggcgta tgcccagtgc gtaaatgaaa cgatcaaaac
       61 ttacccgctt ccggctttgg attgatgcga ccaatttatg cgccgatata tcagcaaaac
      121 gctcaagcgt aagcacatcg tcaacgctta cgctaaagag atcggctgca tcttgaatta
      181 ccttggcatc gagcagcgaa tcaacacttt tttcaccgac gccttcaata tcaaatgcat
      241 ccttgctggc aaaatgaatg atccgcccgc gctcaagcgc cggacagtca aagtttatgc
      301 agcgccaaat tgcctcatgc ggttctttaa aaagaatttg gttgcatacc gggcagcggg
      361 ttggcatgac gaaagcggtt tcattgccag ttcgtagctt tggcaaactt tgcacaactt
      421 caggtatgat atcgccggct ttttgcacga ccaccgtatc gccaacacga atatccttgc
      481 gtgcgacctc accggcattg tgcagggtcg ccatttgaac cgttgatccg gcaataagga
      541 ccggctccaa aaccgcaaaa ggggtggcgg caccggtccg gccaaggctc accataatat
      601 tttttagctt cgtggtagcc tgctccgccg ggaatttata tgccgcagac ccccggggtg
      661 ccttaccaac cacaccaagc gctcggtaca ccgagctggt gttcacggta agcaccacgc
      721 catcggtgcc ataaggaaga tatttgcgct tctcctccca ttccgctgtg tattgcataa
      781 tgccttccac cccttggacc gtttgggcat tggggtcgag tcggaatcca agttgggccg
      841 ccagcaaatg ttccctttca tgagtgtcta ccgaatctgg cggagtggca atgccataca
      901 catgaaaact taactgccgc tcatcaacca acttcggatc gagttggcga atcgtaccgg
      961 ccgctgtgtt gcgggggttt gcgaataatg gttttccatc acgttctctt tcagtattta
     1021 agcgctgaaa aacctcctta tacattaaaa cctcgccgcg aacctcaaac ctgccgcgat
     1081 aaacttcagc tggcacatct gccgcagcac gcaaacgcag cggaacggta ttaatggtgc
     1141 gtagattagc ggttacgtcc tccccaagcc gcccatcgcc tcgggtaagc ccttggataa
     1201 aaatcccgtc ttcgtagatg agcgaggcgg ctaaaccatc catttttatc tcggcatata
     1261 cattcccatc gaactctgga gcctgcttgc tagtgcgcgc cagccaggct tcaacttcgg
     1321 acctatcaaa aagatcattt aagctcagca tcggaatttg atgcggcgcc ggtgtaaact
     1381 gcgcactcgg ctttccggca acgcgctgcg taggcgaatc aggggttatg agatcaggaa
     1441 actcttgctc aagctggcta agttcgtgct ttaaactgtc ggccgcagcc tccggcatcg
     1501 tcgatgtgtc taaaacatgg taatggtagc ggtagtcatt tatttgctcg cgcagcttat
     1561 cgatccgctg gcttgcttcc gtgcgattca ccggcgcatg ttccgaagat agcgatagag
     1621 atataagttg ctaataggaa caaccagcaa tgtagccgtt aattgaagaa gcggctccca
     1681 aattggccat tgcgtgagat tctgtaaaaa gaggaaccca ataagcggca ctgccatcag
     1741 ccccagcaaa aacagggtaa gcgctgccaa ataaccaagc gttttccagg tacgaccagc
     1801 ggttagcctc catgaagccc ttaccgcctc aagcgggcca atttccgact cgaacaccaa
     1861 gtaaagtgcc cacatgcttt tgggcagcaa ccataagcta ggcagggcga tgatgatggc
     1921 cagtccgaca agtaagccca gttcggccga tccaagctgg gcattcgggg caatgacgcc
     1981 ggcggcaaca ataaataatc ctaacagaaa aaagagaagc gtcagaacca atacaactga
     2041 agctaaaaaa agccggagaa acgcctgcga gctttgataa tatatcgtgc gaacattcgg
     2101 cagctcttcg cccttaataa ccttaatgat ggcataaata atcgctgtat tcataatcag
     2161 ttgagcaaaa cttacataag ccgccgccgt ggtgtcgctt gaagtctcca ctatcttaaa
     2221 agtcgttagc agagctaccg gcaaactcac aaccaacacg accccgacca gcaacgtcca
     2281 gtgttgtcga taatcgcgta aagcttgctt aaataatcgc cacggggatt cttgcgcttt
     2341 agccacttag accttgtctc ccatttcccg cagccgctta atgcgctccg cagccggcgg
     2401 atgggtgcta aaaagggatg ccataaaaga ccctactgat ggcaatggat tcgaaaagta
     2461 catgtgcgca ttagcggttg aggcgtgagc cagcggttgt tgctctgatg ccgccgaaat
     2521 tttttccaaa gctctggcca atccctccgg ataacgcgtt aacagcgccc cggacgcatc
     2581 ggctaaatac tcgcgcttgc gggaaaccgc cagttgcagc aatccagcga taagcggagc
     2641 cagcaatgat gctacaatcg ccacaatcgc tagtatcgga ttacctcctt cacggcggcc
     2701 gcccccaccc caactcatat gcaaaaagaa gttgctgaca agtgatataa ccgttaccag
     2761 caccattaca atcgccataa aacgaatatc gtagttgcca acgtggctca tttcatgagc
     2821 taaaacccct tcaagctctt ccttatcaag aagctggagt agtcctgaag ttaccgccac
     2881 cacggcattc ttggggttgc ggccggtagc gaatgcattg ggagatggat catcgataat
     2941 ataaactttt ggcattggaa gcccggctgt aatcgataaa ttttcaacca accggtacag
     3001 ctccggagca tctttcttgg caaccggacg ggcaccagct atggccaggg cgatgctagc
     3061 cgaaaaccgg tagctaataa gagcgtaaac cagtgcaaaa atactaactc caagaaatat
     3121 tcccggttga ttggtcgcta aactgtagct atagcccaaa accgaaatca gaatcacaaa
     3181 agcagtaact aagagaatcg acttacgttt atttgaagca atctgggaat acaatggtta
     3241 accctcgtta tggcgcgacg acggctgagt cgggtcaata cttataaccg gctggctgcc
     3301 aacactatca actttcagtg atgccacctc aggtatatga tgatgtgttt ccctatggtt
     3361 ctgatgatga tcttcatgat gttcttcatg ctcagaatgc tcgtctgact cctcggaatg
     3421 atggtcttcg tgctctgact cgcgctcgcc aaaacgatcc gaacgcatcc gttcaagcaa
     3481 gttacgtaat aattcttcgg tgctgccggt atcaataccg ggataaattt tatgcacaag
     3541 ctcaagctcc gccatcaact taattgatgg atcaaggttg gcggggcggt tagcctgcaa
     3601 gtcagctgca acggttgctg gaatgtccac aaccgcttgg cgtagatgcc aaaccagaac
     3661 attggtgtcg acatcactaa accgctcgca cagctgcact agctgtttcg atagctcaag
     3721 cgaatccttc caggagtagg ctttggtaat gtcatccatg ctcgagtatc cgcctcctta
     3781 aaactgaaca gctaccggct tttcaatttc tgtttgattg tccgcctcaa agaattcacg
     3841 atccttaaag cccatcatgc ctccaaccat attggttgga aaaacctgaa gtttggtatt
     3901 aaagtcgcga acgttgccat tgtagaaacg ccgagccgcc tgaattttat cctcggtgtc
     3961 taccagttcc tccgacagct gctgaaagtt ttgacttgcc tttaaatctg gataggcctc
     4021 actaacggca aacaagcttt taagcgtttc ggctagcata ttctcagctt gggctttttc
     4081 atgaaccgag gtagctccca tcgctgcgct gcgcgccgtg gtgacatctt gaaacacttt
     4141 tgactcatgg gtggcatacc ccttcacact cgttaccaag ttcggaataa gatcataccg
     4201 gcgttttaat tggacattaa tatcgctcca ggattcatca acacggtttt ttgagcttac
     4261 taagccgtta taaattacga tcagggctat tgctataacc gcaatgacgc cgagtactat
     4321 ccagagttcc atcaaaatta tcctttattt tagcttttat tatagcagtt ttcggcactt
     4381 ttggcagtgt gaagcactaa ctttactcca atggtgggag aggagggact cgaaccctca
     4441 agccttatta aggcagcgga ttttaagtcc gccgtgtata ccattccacc actctccctg
     4501 gaggcaccga ccggaattga accggtgtac aaggttttgc agacctctgc gtaaccactc
     4561 cgccacggtg ccaagcctcc aaattataca tggctacctg ttggctgtca gcataagggt
     4621 attccgacct tccagattgg agcccaatcc gtctatggta taaattaagg acgctccaag
     4681 gaccgacgaa aggagatcca tgtcgcaaaa gcggttcgaa cgggtcacag gggcagccat
     4741 cggagcaatc gcatggttcg ctcttaccta tcccgaagcc actaccaacc acaacatgat
     4801 cgacatcgac catccgttct ggaagttcac cttcccagag cggggctacg tcatcatcct
     4861 cgaggttgac cgggatgaca atcccactaa cctcctgtgt cgtacgcggt tccaccagcc
     4921 gggaggcaga aatcggcaca gcctcccact gagggatccc atcagagagg tgtatgtctt
     4981 caccgacgcg cagatggtct tctcggaaga gcatggtctc agcgtgagac agctccaaga
     5041 gatcgttcaa cggaccatgc ggcaggccgt cgaagccgct gaagcagcgg tcgccgatgt
     5101 gtcaggtgaa gccattcctg acgctgcagt ctccgttcgc ggaacggagt agccatggcc
     5161 gactggcaag atcccaaggc cgcgctctca tctcttgagg gcgcggctct gggagccaca
     5221 ctgacagcac ttataggtat ctcttggtag cataaccgtt ctaaagtata cttttcttgt
     5281 gaagctgcat agaaagctca atcgaattgc gacggctcga cgttttaata tcaagatgct
     5341 cattgttttt atcatactgg catccgtggg tggcgtgtat gtggtatatc gttctaacgc
     5401 ggctacctat ggacacaacc cctacatgta cgccacagct gttgacgttc gaaccggggc
     5461 gacggttcca aatgttgata ttcgtgttag atcaacagat gatcaacgct attgcatcac
     5521 cccctacaca cctaccgacg gaggcggcca tacctacttt tggagttgtc agcttggagg
     5581 agtagatgga gccgggcgcg tctttgacca ctattatgtc tattacgccg ctgccccgcc
     5641 cggctatcaa attgccgatc atgttggc
//
LOCUS       DASVGB010000083         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_45625, whole genome shotgun sequence.
ACCESSION   DASVGB010000083 DASVGB010000000
VERSION     DASVGB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45625"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VF272_02565"
     CDS             complement(<1..679)
                     /locus_tag="VF272_02565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7259791.1"
                     /translation="MKVSIIIPCLNEEHYVAKLLCDLERQTHNDFEVILMDADSIDKT
                     LKVVKPFRQSLPLTIIEHAPKGVANARNIGADHAVSDWLLFMDADVTVTPNFLTTFLE
                     AARKHEVDAASSKFTADSHKKLDHFGAWLHFRYMKLFERTSYPLAPGGCILIRRDIHH
                     KVSGFDPRVMSEDFDYITRCAAAGASFKFITDTSFKVSVRRYEKDGRIRLFLKLMVYE
                     AHRLIYGK"
     gene            777..2834
                     /locus_tag="VF272_02570"
     CDS             777..2834
                     /locus_tag="VF272_02570"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7259792.1"
                     /translation="MVMVLRARPKVLIMTASAGGGHVAAAKALEAGFKQVYGSTYAVE
                     VCDVFTDPRLKLLLPIDRLAVPAYSNSVKIFRSYPYKLFFNFSNATPGIMNRFFSSVF
                     RDRVRDFLQDVDPDVIISTYPIISYATGRILDEGWPKPVPIISLVTDSGDVHRLWLGG
                     ADDAILVSTRDTIQFAVKNGVARDKLYFLGFPVDAAYNNLPSKQAARQKLGLNTDKLT
                     FVLSGGGLGLSANLYQLAKLLSQKKLDGQFIFVAGQNTDLYQRLQDLDYASPTKVFGY
                     VDTMPELVAAADVMVGKAGWITLNEAMIAAKPTIIIDIIKGQEESNAAFVERHGIGII
                     CQKPSLAYRAIVNLIKEPQQVKVYQRRLRKLRLEPEASLTIPRFVVERFIEEYPKAVY
                     HQLTIHGRRVNYAVLNPKAKQTIVMIHGMRGNNRGLLKLAQQFSGYRVIIPDLPGYGA
                     SETLSVAHSLDNYANFLEAFCAALHLHAFDLVGHSFGAGVCLVAASKRPASLKTLTLI
                     TPVSVSEHWLSKLATAYYQIAQYLPPHVRRRWLASKGINRLESYFLLKGSNPDQKKQI
                     IRARLSDLNELNAQVVLESAENIHYSRYFEQAGLINVPTLIIGGGQDQIAPPESTDWL
                     AETIAGSQLINFPQGGHLLPLEEPELVGTQILVFIKAPTAVAGATAEHLVTTSEGVLT
                     QPS"
     gene            3077..>3493
                     /locus_tag="VF272_02575"
     CDS             3077..>3493
                     /locus_tag="VF272_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7259793.1"
                     /translation="MPVNQRQVFENISEVQPLPNLIQNQIQSYEWFKAEGLEELFAAV
                     NPIEDFTGKNLALYFRDFHFEEPKFDELTAKSKNATFEVPLKAKVELVNKETGEVKTQ
                     EIFLGDYPWMTKRGTFIINGVERVVVSQLVRSPGVFF"
BASE COUNT          901 a          807 c          864 g          921 t
ORIGIN      
        1 ttttgccgta gattaaacgg tgggcctcgt agaccatcaa tttgagaaaa aggcggattc
       61 tgccgtcctt ctcatagcgg cgtaccgata ctttaaacga ggtatcggta ataaatttaa
      121 aactggctcc tgcggcggcg cagcgagtaa tataatcaaa atcttcgctc ataacccgcg
      181 ggtcaaaacc gctgacctta tgatggatat cacggcggat cagtatgcac ccgcccggtg
      241 ccagagggta tgatgttcgc tcaaataatt tcatgtagcg gaaatgcagc caggctccaa
      301 aatgatcaag ttttttgtgg ctgtcagcgg taaattttga tgatgccgca tcaacttcat
      361 gtttgcgagc cgcttctaaa aaggttgtca gaaaattagg agttacggtt acatcggcat
      421 ccataaacaa cagccagtcc gacacggcat ggtcggcacc aatattccgc gcattcgcta
      481 cgcccttagg ggcatgctcg ataattgtta acggtaggct ctgccgaaaa ggttttacca
      541 ctttaagggt tttatcaata gagtcggcat ccatgaggat tacttcaaaa tcgttgtggg
      601 tctgccgttc aagatcacat aacagtttgg caacgtagtg ctcttcattt aaacatggaa
      661 taataatcga tactttcaca gtaacctcgc ctgaatcgtt agagctatgc tttacactat
      721 agtaatgcta aacgccacgg cgactcaagc cggtttacca ttgatacgtt ataattatgg
      781 tcatggtgtt acgggctcga cccaaagttt taattatgac ggcttctgct ggaggcggtc
      841 acgtagctgc agccaaagcc ctggaggcag gttttaaaca agtctatggc tcaacctatg
      901 ccgtagaggt gtgcgatgtg tttacggatc cgcgtcttaa gttgctgttg ccgattgatc
      961 gattggcagt tccggcctat tcaaacagcg ttaaaatttt tcggtcttac ccttataaac
     1021 tgtttttcaa cttttcaaat gccactcctg gcataatgaa ccgatttttt tcctcggtgt
     1081 tcagagaccg ggttcgcgac tttctccagg atgtggatcc cgacgttatt atttcaacct
     1141 atccgattat cagttacgct accgggcgaa ttttagatga aggctggccc aagcccgtcc
     1201 cgattatcag tctggtaacc gattccggcg acgtacaccg gctgtggctg gggggtgccg
     1261 atgacgcgat attagtttca acccgcgata caattcaatt tgcagttaag aatggcgtgg
     1321 ctcgggataa actctatttt cttggttttc cggttgatgc ggcttacaat aacctgccct
     1381 caaaacaggc ggcgaggcaa aagttagggc tcaataccga caaactaacg tttgtgctca
     1441 gtggcggagg cctagggttg tcggctaact tgtaccagct tgccaagcta ttaagtcaaa
     1501 aaaaactgga tggccaattt atttttgtgg ccgggcagaa cacagacctt taccagcgct
     1561 tgcaggattt agattatgcc agcccaacaa aggtttttgg atacgttgat accatgcccg
     1621 aattggttgc ggcggccgat gtgatggttg ggaaggcggg ctggattacc ctcaatgaag
     1681 cgatgattgc ggctaagccg actattatta ttgacattat caaagggcag gaagagtcaa
     1741 atgcggcatt tgtagagcgc cacggcatcg gcattatttg tcagaaaccg tcgctagcct
     1801 atcgggcgat tgtcaatttg attaaagaac ctcagcaggt gaaggtttat caacggcggt
     1861 tgcgtaagct taggctggag ccggaagcca gtctaaccat tccgcgcttt gttgttgagc
     1921 ggtttattga ggagtatcca aaagcggttt atcaccaact caccattcat ggccgacggg
     1981 taaactacgc ggtactaaat cctaaggcta aacaaacaat agtaatgatc catggcatgc
     2041 gcggtaacaa tcgcggcttg ctcaagctgg cgcagcagtt ttcgggctac cgagttataa
     2101 ttcctgatct tccgggctac ggggcatccg aaacattatc ggtggcgcac agtttggata
     2161 actacgccaa ctttttagag gcgttctgcg ccgccctgca cctgcatgcg tttgatttgg
     2221 tgggacactc attcggagcc ggagtttgtc tggtggcagc ctcaaaacgc ccagcctcgt
     2281 taaaaacgct tacattgatc accccggttt cggtttcaga acactggctt agcaagctgg
     2341 ccacggctta ttaccaaatc gcccaatact tgccgccgca tgttcgccgt cgatggcttg
     2401 cgagcaaggg tattaaccgg ctggaaagtt acttcctatt aaagggcagc aatcccgatc
     2461 agaaaaagca aattattcga gcgcgcctaa gcgatttaaa tgaacttaat gctcaggtgg
     2521 tattagagag tgctgagaat attcactata gccgctattt tgagcaagct ggtttaatta
     2581 acgtacccac cttgattatt ggaggcggac aggatcagat cgcccctccc gaaagtactg
     2641 attggctggc ggaaaccata gcgggcagtc agctcataaa tttcccgcag ggcggtcatc
     2701 tcctgcccct tgaggagccg gaattggtag gcactcaaat tcttgttttc attaaagctc
     2761 cgacagcagt ggctggagcg accgcagaac accttgttac aaccagcgag ggagtgttga
     2821 cacaaccgtc ttaatcatct aatgtattct acatagagga agcttaaatc caagtaggaa
     2881 acccctacct gctttttttg cgtgtaagat tgccttggct atccattcgt acagccgcgg
     2941 aagtcttaga cttaaaagca gccaagggtc catgcagctg atttagaaaa accttttgat
     3001 ttaggtgtcg tataagggag caaccaggac tctcaggcgg cgcgcagcac accattaatc
     3061 aatcaaggag tatcttttgc cagttaatca gcgtcaagta ttcgaaaaca tttccgaggt
     3121 tcagccactg ccaaacctca ttcagaatca aatccaatcg tacgaatggt ttaaagccga
     3181 aggtttagag gagctgtttg cggcggttaa tccgatcgag gattttaccg gcaaaaactt
     3241 agcactatac tttcgggatt ttcattttga agagcccaag tttgatgagc tcaccgccaa
     3301 atccaagaat gccacatttg aagtcccgtt aaaggccaag gtggaattgg tgaataaaga
     3361 gaccggcgag gtaaagacac aggagatttt cctaggtgat tatccctgga tgaccaagcg
     3421 cggcaccttc atcattaatg gtgttgagcg ggtggtggtg agtcagctgg tgcgctcacc
     3481 gggtgtcttc ttt
//
LOCUS       DASVGB010000084         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1008360, whole genome shotgun sequence.
ACCESSION   DASVGB010000084 DASVGB010000000
VERSION     DASVGB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008360"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="rsmD"
                     /locus_tag="VF272_02580"
     CDS             complement(<1..435)
                     /gene="rsmD"
                     /locus_tag="VF272_02580"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7259794.1"
                     /translation="MRIVGGVYGGRSLTAPKGNGTRPMTDKVRAALFDSLGAISGASV
                     LDVYAGSGAVGFEALSRGAGNVQAIEHARSAIQAIQANQSALDLTWGYALHQKKVEAW
                     LAHRSNEVFDIVIADPPYEYLKTDVLEKLGILLKPQGIMVVSH"
     gene            complement(435..1274)
                     /locus_tag="VF272_02585"
     CDS             complement(435..1274)
                     /locus_tag="VF272_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259795.1"
                     /translation="MYSGFITSKRIVPAVGVHQRFNAASYRMIAPYFAEGTFPTLKQL
                     MHFEGMNGPDGLKVKSPGHHDPGHLYNPETGAGDIPRLITDHYRNLVSALRSKDLVRA
                     GFDAAWLAHYICDGLTPAHHFPLNENVAKQSEHSKRIPKRYKYTVIVPGINFFDTIRR
                     SWAVWGGKGLLTTHFNFEVGVAAALVGFRLKADLNHEQLAKARSVSLLPFFKSEAQSI
                     AELEMYKSFYVSGWNSELARMVKNRLAPQIVQTIAIAWLLAYLEAGLESAKLAQPRRS
                     KKR"
     gene            complement(1301..>2202)
                     /locus_tag="VF272_02590"
     CDS             complement(1301..>2202)
                     /locus_tag="VF272_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7259796.1"
                     /translation="GLVIIDEQHRFGVEQRQILKQKAGRLPHLLSMTATPIPRSLALT
                     VYGDLDISLIDSLPPNRQPIATKILKPNDRTAAYGFIDSEIAKGRQVFVVCPLIEDSD
                     VLGAKSVTTEAERLQTGAFSHRRIGVVHGKLKPADRDEVMEQFTGGELDILVATSVIE
                     VGIDIANASIMLIEGAERFGLAALHQLRGRVGRGAHHSYCFIASDSSTPGAIQRLQAL
                     ERTNDGFRLAQIDLELRGPGQVYGRRQHGMLELEMADLGDVRLVAAVRKAAERFVSDS
                     KDMLQYPQVVARVNRLKHLTSLD"
BASE COUNT          545 a          601 c          535 g          521 t
ORIGIN      
        1 atgcgacacc accatgatac cttggggctt aagcagtata ccgagttttt ccaatacatc
       61 agtcttgaga tattcatacg gcgggtcggc gatcacgata tcaaacacct cattggagcg
      121 atgtgccaac caggcttcca ccttcttttg atgtaacgca tacccccatg taagatcaag
      181 ggccgattga ttggcttgga tggcttgaat ggctgaacga gcatgttcaa ttgcctgaac
      241 attgccagcg ccacggctga gcgcctcaaa ccccaccgcg ccgctgccag cgtagacatc
      301 aagaacgctt gcgccgctta tagctccaag gctatcaaac aacgccgccc ttaccttgtc
      361 ggtcatcggg cgagtaccgt tgcccttggg ggcggttagg cttctccccc cgtagactcc
      421 accgacaatt cgcattagcg ttttttcgat cgccgcggtt gggctagctt agcagactct
      481 aatccggcct ccaaataagc aagcagccag gcaatggcaa tggtttgaac aatctgcgga
      541 gccagccgat ttttaaccat gcgtgccagt tcggagttcc atcctgaaac gtaaaagctc
      601 ttatacatct ccaattcggc aatcgattgg gcctcggact taaaaaaggg taataggctt
      661 acgctccgag ccttagccag ttgttcatgg ttcagatcgg cttttaaacg gaagccgaca
      721 agcgcagccg ccacgccaac ttcaaaatta aaatgtgtgg ttagcaaacc tttacccccc
      781 caaaccgccc agctgcgccg aatggtatca aagaaattga tgccgggaac aatcacggta
      841 tatttgtagc gtttggggat gcgtttggag tgctctgact gtttagcaac attttcattt
      901 aacggaaaat ggtgcgccgg ggtcaggcca tcacagatgt aatgagccag ccaagcagca
      961 tcaaatcccg ctctcaccaa gtctttggag cgtagggctg aaaccagatt gcgatagtga
     1021 tctgttatga gccgggggat gtcacctgca ccggtttccg ggttgtagag atgaccgggg
     1081 tcatgatggc cgggagactt aacctttaag ccgtcagggc cgttcatccc ctcaaagtgc
     1141 ataagctgct taagcgtggg aaaggtgcct tcggcaaaat agggagcaat catccgatag
     1201 gaggctgcgt taaagcgttg atgtacccca accgcgggca caatccgctt cgacgtaata
     1261 aagcctgagt acattaaact ctccagtctc cttgcttgat ttaatcgagc gaagtaaggt
     1321 gtttcaacct gttaactctc gcgacaactt gcggatattg tagcatgtct tttgaatcgc
     1381 tcacaaaccg ttcagcagct ttacgtacag ccgctaccaa tcgtacatcg ccgagatccg
     1441 ccatttccaa ttcaagcatg ccgtgctgac gacggccata gacctggccc ggcccgcgta
     1501 attccaaatc aatttgagcc agtcgaaacc cgtcattggt tcgctccaat gcttgcaacc
     1561 gctgaattgc ccctggtgtg ctcgaatcgc tggcaataaa acaataggaa tgatgggcgc
     1621 cgcgccctac cctgccccgc agttgatgca aggcagctag tccgaaccgt tcggcacctt
     1681 caatgagcat tatgctggcg ttagcaatat cgatgccaac ttcaatcacg ctggtagcca
     1741 cgagaatatc cagctcccca ccagtaaatt gctccataac ctcatcgcga tcagccggtt
     1801 taagcttgcc gtgcactact ccaattcggc gatggctaaa agctccggtt tgtaagcgct
     1861 cagcctcggt ggtaacgctt ttagcgccta acacatccga atcttcaatg agtggacaca
     1921 ccacaaatac ctgacgccct tttgcaattt cactgtcaat aaagccgtaa gcggcggtac
     1981 gatcgttagg cttaagaatc ttagttgcaa tcggctgccg atttggcggt agcgaatcaa
     2041 tcagggatat gtccagatcg ccgtaaaccg tcagagccaa gctacgcgga attggcgtgg
     2101 cagtcatact taagagatgg ggcagccggc cggccttttg cttaagtatt tggcgctgtt
     2161 ccacgccaaa acggtgctgt tcatcgataa tcaccaaccc ta
//
LOCUS       DASVGB010000085         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_512614, whole genome shotgun sequence.
ACCESSION   DASVGB010000085 DASVGB010000000
VERSION     DASVGB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512614"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VF272_02595"
     CDS             <1..1382
                     /locus_tag="VF272_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018298941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7259797.1"
                     /translation="RLYFEELTLERMQDIADFEQPHGIVVSVGGQIANNLALPLAKAG
                     YPIMGTSPDQIDRAEDRQKFSSMLSKLGVDQPTWEEVSTLAKAKAFAAKVGYPVIIRP
                     SYVLSGGAMNVVSNEDELAFFLTEAAELSPDHPVVISQFILNSKEIEIDGVAKDGRLQ
                     IYAISEHIENAGVHSGDATVVFPAQRLYLETVRRAKLITKQIIKNLDITGPFNVQFIA
                     KDNDLKVIECNVRASRSFPFVSKVTGHNFIAIAAESILGIESSTAYQTLEYDYVGVKS
                     PQFSYNRLKGADPVAGVEMASTGEVACFGDNLQEAFYRSWLATEQRIKQKTLFLSLSD
                     VNKYRFVEEAADIVKQGWKIYATPGTHDYLKDNGIASIQLHKIVEQTEPSIISAIDKQ
                     QIGLMISIPSSNRDSNDAYKIRRLAIDSHIPLITNAETGRLLLRCLGDPIVLQLQPKA
                     WQDYVKEEV"
     gene            1379..2299
                     /gene="pyrB"
                     /locus_tag="VF272_02600"
     CDS             1379..2299
                     /gene="pyrB"
                     /locus_tag="VF272_02600"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX7259798.1"
                     /translation="MKHVLNVEQFTPEDLTRLFKEADIMREHVADPAKSLELARKHHG
                     KQICTLFYEPSTRTRFSFESGALKLGMGVVSTENAGEFSSASKGETITDTMQMLNGYQ
                     FAAVVIRHPQTGIVATAAEASDAPIINAGDGKGDHPTQSLLDLYTIHRHFRRLENLRI
                     VMGGDLQNGRTVRSLAKLLSKYSGNHITFVSLPQLQMDDDVKAVLDASGTTFNELHAI
                     DDAFADADVVYWTRLQAERLDPANVLANGGFTLNAAVLTGLPEKAIIMHPLPRVDEIA
                     VEVDADPRAKYFEQAANGLYVRMALLDHLV"
     gene            complement(2300..>2744)
                     /locus_tag="VF272_02605"
     CDS             complement(2300..>2744)
                     /locus_tag="VF272_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259799.1"
                     /translation="ASTIRTYFRIAVYTWCMLVLAAYTGFFIYEGILHTQELSTLLLA
                     LPFLFVQLSAVFTMLVLFLPPKTFDWSTITQSYRDGFAKILSYTQLRPLPIVATVAFL
                     AISVGVMAIGSQLGQTGLAFALIYAAALLKPSLLYSKPAPTTPQA"
BASE COUNT          708 a          646 c          731 g          659 t
ORIGIN      
        1 agcgcctcta ctttgaggag ctaaccctgg agcgcatgca agacattgcc gattttgagc
       61 agccccacgg cattgtggtg tcggttggcg gccaaatcgc caataattta gcgctgccgc
      121 tggcgaaagc cggatatccg atcatgggta cttcacccga ccagatcgac cgggccgaag
      181 accgccagaa attctcgagt atgctttcaa aattaggcgt cgaccagccg acctgggaag
      241 aagtgagtac attggcgaaa gccaaagctt ttgctgctaa agtcgggtat ccggttatta
      301 tccgcccttc atacgtgctc tctggcgggg ctatgaatgt agtatcaaat gaagacgagc
      361 tggcattttt cttgaccgag gctgccgaac tgtcgcccga ccacccggtg gttatctcac
      421 agtttattct taactccaag gaaattgaga ttgacggggt tgccaaggac ggccgcctgc
      481 aaatatatgc catatccgag catatcgaaa atgccggcgt tcattctggc gacgccacgg
      541 tggtttttcc ggctcaacgc ttgtatctcg aaacggtgcg ccgggctaaa ttaattacca
      601 agcaaatcat caaaaatctg gatattaccg gcccgtttaa tgttcaattt attgcgaaag
      661 acaacgacct gaaagttatt gagtgtaacg tccgggcgtc gcgctcattt cctttcgttt
      721 caaaagttac cggccataac ttcattgcga ttgccgctga atcgatcctg gggattgaaa
      781 gcagcaccgc ttaccagact ctggaatatg actatgtcgg ggtaaagagt ccgcagttct
      841 cctataaccg gctgaaaggg gccgacccgg ttgccggtgt tgaaatggcc tccaccggag
      901 aagtggcctg ttttggggat aacctacaag aggcatttta tcggtcatgg ttggccaccg
      961 aacaacggat taagcaaaag accttgtttt taagcctttc ggacgttaat aagtacaggt
     1021 ttgtagaaga agccgccgat attgttaagc agggatggaa aatttatgcc acgcccggca
     1081 cccatgatta cttaaaggat aatgggattg cctcgattca actgcataaa attgtggagc
     1141 aaaccgaacc aagtattatc tccgcaatcg ataagcagca aattggcctg atgatcagca
     1201 ttccgtcatc taatcgcgac agcaacgatg cctataagat tcgtcgctta gcaattgata
     1261 gccatatccc gctgataacc aatgctgaaa ccggccgctt gctcctgcgt tgtttgggtg
     1321 acccgatagt gttgcagctt cagcctaaag cctggcagga ttatgtaaag gaggaagtat
     1381 gaagcacgta ttgaatgttg agcaatttac gcccgaagat ttaaccaggc tcttcaagga
     1441 agctgacata atgcgggaac atgtggccga ccctgccaaa agcctggagt tggctcgcaa
     1501 gcatcatgga aagcaaatct gtaccttgtt ctatgagcca tcgacccgta cgcgcttcag
     1561 ttttgaatcc ggcgccttga aattaggcat gggtgtggtg agtactgaga atgccgggga
     1621 gttttcctcg gcctctaagg gtgaaaccat caccgatacc atgcagatgc ttaatggtta
     1681 tcagtttgca gccgtggtga ttcgccatcc ccaaaccggg attgtagcaa cagctgccga
     1741 ggcgagcgat gcgccgatta ttaatgccgg cgacggcaaa ggcgatcacc ccactcagag
     1801 cttgcttgat ctgtatacga ttcatcggca ctttaggcgt cttgaaaatc ttaggatcgt
     1861 gatggggggg gatttgcaaa acgggcggac ggtgcgttcc ttggcaaaat tgctttcaaa
     1921 atactctggc aatcatatta cgtttgtttc gcttccgcag ttgcagatgg acgatgatgt
     1981 aaaagcggta cttgatgcca gcggcaccac atttaatgaa ctgcatgcca ttgacgacgc
     2041 ttttgccgat gccgatgtgg tgtattggac acggctacaa gccgaacgtc ttgatcccgc
     2101 gaatgttcta gcgaatggag gttttaccct taatgcagcg gtgcttaccg ggctgcctga
     2161 aaaggctatt atcatgcatc cgctcccaag agtcgacgaa atcgcggtag aggttgacgc
     2221 tgatccccga gccaagtact tcgagcaggc agccaacggt ctgtacgtcc gcatggctct
     2281 gctcgatcac cttgtgtagc taggcttgcg gagttgttgg tgctggcttg gaatagagca
     2341 gtgagggctt gagtagagcg gcagcataga tcaatgcaaa ggctaggccg gtctgaccaa
     2401 gctgcgaacc aatcgccatt acgccaactg aaatggccaa aaacgctaca gttgcgacga
     2461 tcggaagtgg tctaagctgc gtgtaactca agatctttgc gaagccatcg cggtatgatt
     2521 gcgtaatagt ggaccagtcg aaagtttttg ggggtaaaaa cagaacgagc atggtaaaga
     2581 ccgcggatag ttgaacgaaa aggaaaggta gtgcaagcaa taacgtgctt aattcttgag
     2641 tgtgcaggat cccttcgtaa ataaaaaatc cggtataggc ggcaagcacc agcatgcacc
     2701 acgtatagac ggcgatacgg aagtaggtcc gaatcgttga ggcg
//
LOCUS       DASVGB010000086         6270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1173653, whole genome shotgun sequence.
ACCESSION   DASVGB010000086 DASVGB010000000
VERSION     DASVGB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173653"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VF272_02610"
     CDS             complement(<1..1004)
                     /locus_tag="VF272_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7259800.1"
                     /translation="MPQNLKTTLRKIYLFCFLDDFVLLYPFYSIYMAGKGLSLFQIST
                     LLILWSLTDLIANIPLGVLADKYSRKNMLAIGQILKALSFISWVLFPSYEGFALGFVL
                     WGIGGAFIDGTFDALVYDELKAVNQEKHYVKVIGRAETVAFTGNLLATVLAGAAILLG
                     YGFVFAGSIFAVVVSAAVVYRLPETPRFGKIGDTRYFSMMRQGIKEATHNRTILAIIL
                     LGGFIAAVYGSLEEYLPLVIQETGLNLSLISLAVAATVAAAGVGTLVAYRFEKFSTPS
                     IMILLALSGSLLLAAGAWVGAGAIALLVGYTFIIRMLQAVFNGKLQHSITSGLRATIS
                     S"
     gene            complement(1028..2326)
                     /locus_tag="VF272_02615"
     CDS             complement(1028..2326)
                     /locus_tag="VF272_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259801.1"
                     /translation="MSSSGGSGLGSGSGGSRGRAESGPGHGEAAFTAAEAEGEGVSGP
                     IADFEALTRPEDVDEADLQAAAELADMDRIRAAVEDKHGSNRPGDGASSEAVTWDQAV
                     EQLRAGRAEALDLPNDATLEEVTEQVAAGIKGRNRAVSFGGARNAGQKVPEDQAPEDQ
                     APEDRGEAEVSAEQSGTEPITTHEEARAASERYQNPTPEQAAAMNKRSGEMFQSLLEQ
                     ESQSGVESVEAPERVSADIENMDFIALSNYMISFRQGEMPNGQKVSEFREIAQGSNGE
                     WLLKITRTTDAGHVQEVKGYQSSSLRDLEREATRGLVDDGEDLEKSEELRCDLEWNEE
                     AQGYYCTALDENGAKVDFPEDLKDMIASELRPNWTVVLEGSEALIKKADGSRDGLLDQ
                     VLDMSFDNDLSKDREMNPQDAVGVRSGVDNGVNEYNRQFA"
     gene            complement(2426..2602)
                     /locus_tag="VF272_02620"
     CDS             complement(2426..2602)
                     /locus_tag="VF272_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259802.1"
                     /translation="MSHSRGRRIKALKANPYEDPEVFYMSLEEAKDMISSKYEFLAER
                     YERLKPKKPRSTQG"
     gene            complement(2724..3167)
                     /locus_tag="VF272_02625"
     CDS             complement(2724..3167)
                     /locus_tag="VF272_02625"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7259803.1"
                     /translation="MLATVPHPVLDRLSLSLVIAVVAIPITYMQVAQPSIADIQKALG
                     IPPQTATLQPAAPVPLATPSKPAPVPVAPATVTPKAAPAKPAAKTAFTNSFVNLRAGK
                     SVSTPIIATLEAGTTVQLRDDADTTWQGVTYQGKNGYIYRSYIQY"
     gene            complement(3170..3604)
                     /locus_tag="VF272_02630"
     CDS             complement(3170..3604)
                     /locus_tag="VF272_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259804.1"
                     /translation="MDPNKLRPSRPRTLDSVGSSAARPAGRPAGSAHVIDLREKSSSF
                     NAPAAVDTKPSVVKKKQPERSQVPVAAPPPTPAPSPAQSSKQAATPPPRQRSPQQDRY
                     TDAAFPAKPRFWPAFWRFLFLLILLGLIISLGVYIYFSYYQR"
     gene            complement(3725..3982)
                     /locus_tag="VF272_02635"
     CDS             complement(3725..3982)
                     /locus_tag="VF272_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7259805.1"
                     /translation="MATKLFVGSLAYSVTDAELESAFAEAGTVVSAKVIMDRDTNRSR
                     GFGFVEMSSDEEAQNAVKLLNEKEIGGRRIIVSEARPQQPR"
     gene            complement(4203..5885)
                     /locus_tag="VF272_02640"
     CDS             complement(4203..5885)
                     /locus_tag="VF272_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7259806.1"
                     /translation="MEQSLFYQLSSIMVLAAIVSLIFKALKQPMIIGYIITGFLVGPT
                     FLDITADHDILASFSEIGIVLLLFMIGLGINAAVIKSTGKPVLVTFLLITVGVGAISY
                     GAGAILGFSMQECLIVAVALLFSSTIIVVKALSDRKEQSRLYSQLAIGILLVEDVAAT
                     VALLWVSTSTGGSVTLGDFWLLIVKGMVLGGSLALIGMYLMPRFSKLFAESQELLYIL
                     ALAWGFGVASLFQLSGFSIEVGALFAGVAMAHLPYAQEISNRLKPLRDFFVLLFFIEL
                     GSRMGINNLQAALVPALIFSAIILITKPLIIIASLGALGYTKQTAFRAAVHLSQISEF
                     SIILVVLAQKTDTIRPEITAMVTLTALITIIVSAYLMNYDHKLYRLFEKQLSVFERAE
                     TKKEVRALEHYPLVLIGYQNGGHDFVRTFRAMKKRYVVIDYNPGIIEVLQHQHIVHIY
                     GDSTDTELLDEIGVHRSELIISNITSLETNSLLLSHISSHNTDSVFICHASTYEDAQA
                     LYEKGATYVMMPHLIGTEHLNSFIRHNGSNKAAYEKHRKHQLRQLSKALLAA"
     gene            complement(5973..>6270)
                     /locus_tag="VF272_02645"
     CDS             complement(5973..>6270)
                     /locus_tag="VF272_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HEX7259807.1"
                     /translation="LIVAAILPQSFAQGVVYLAAYALGLSLMLLLISIAGQAFARRLA
                     IASNPDGWFMRSLGFVLIVVGLAVSFGLDRQFQTFVLDQGWYDPIMHIDERLQQ"
BASE COUNT         1625 a         1672 c         1479 g         1494 t
ORIGIN      
        1 gaactgatag ttgcccgcaa tccgctggta atagagtgct ggagtttgcc attaaaaacc
       61 gcctgcagca tgcggatgat gaaggtatag cccacaagta gtgcaatggc gccagctccg
      121 acccaagcgc cagctgccaa caacagcgaa ccggaaagcg ccagaaggat catgatgctc
      181 ggagttgaaa acttctcgaa ccgataggcc acaagcgttc cgactccagc agcagccacg
      241 gtagcggcca ctgctagcga aataagcgaa agattaagcc cggtttcctg aattaccaac
      301 ggcaggtatt cttccaacga accatatacg gcagcaataa accctccgag cagaataatc
      361 gccaggatag tccggttgtg agttgcctcc ttaatgcctt ggcgcatcat tgaaaagtag
      421 cgcgtgtccc caatcttccc aaaacgcggc gtttcaggca atcgatacac caccgctgcc
      481 gaaacaacta ctgcaaatat actcccggcg aagacaaaac cgtaaccaag caaaattgca
      541 gcaccggcta acaccgtagc cagtagattc cctgtaaaag caacggtttc agcccggcca
      601 atcactttca catagtgctt ttcctggttg acggctttta gctcgtcata aaccagggca
      661 tcaaaagtac cgtcgatgaa cgcgccaccg atcccccaca gcacaaaacc aagcgcaaac
      721 ccttcgtagc ttggaaaaag cacccaagaa ataaaactca gcgcttttaa gatctgcccg
      781 atagccagca tattcttgcg cgaatactta tcggccaaaa ctcccagggg aatattggca
      841 atcaggtcgg tcaaagacca taaaatgagg agggtagaaa tttgaaagag gctaagacct
      901 ttacctgcca tgtatatgct gtaaaaggga tacagaagga caaagtcatc cagaaaacag
      961 aaaagataga tcttccgaag agttgttttt aggttttgcg gcatgatcaa actatagcaa
     1021 aagcggctta ggcgaactgg cggttgtatt cattcacgcc gttatcaacc ccagaacgca
     1081 caccaactgc atcttgcgga ttcatttccc ggtcttttga aagatcgtta tcgaaggaca
     1141 tgtcgagtac ttggtccagc agaccatctc ggctaccatc agccttctta ataagcgctt
     1201 ccgacccttc aagaactacc gtccagttgg ggcgtagctc ggaagctatc atgtccttaa
     1261 gatcttctgg aaaatcaacc tttgccccat tttcatcgag ggcggtgcaa tagtaaccct
     1321 gcgcttcctc attccactca agatcacacc gcagctcctc cgatttctca aggtcttcac
     1381 cgtcatccac cagacctctg gttgcttccc tctcaagatc tcgcagtgag gaggattgat
     1441 aacctttaac ttcctgaaca tgcccagcat cagtggtccg ggtaatcttt agcaaccatt
     1501 cgccattgct cccttgagct atttccctga actcactaac tttctgaccg tttggcattt
     1561 ccccctgacg gaaagaaatc atgtaattac tcagtgcaat gaagtccata ttttcaatat
     1621 cagccgacac ccgctctggg gcttctaccg actcgacacc cgactggctt tcctgctcta
     1681 gcaaggattg gaacatctcg cccgatcttt tattcatggc tgctgcttgc tcgggggttg
     1741 gattttgata gcgctccgat gctgctctag cttcttcatg cgttgttatt ggttccgtcc
     1801 cggattgttc agccgaaacc tcagcctcac ctcgatcttc tggagcctga tcttctggag
     1861 cctgatcttc tggaaccttc tggcccgcat ttcttgcgcc gccaaagcta accgctcgat
     1921 ttctcccctt tatgccggca gcaacctgtt cggtgacttc ctccagggtg gcatcattcg
     1981 ggaggtcaag ggcttctgct cgccctgctc gtagctgctc aacagcctga tcccaagtca
     2041 cagcttcgct gctcgcaccg tcaccaggac gattgctgcc atgcttgtct tccacggcag
     2101 cccgaatccg atccatgtcg gcaagctctg cggcggcctg aaggtcagct tcatcaacat
     2161 cttccggccg ggttaatgcc tcgaagtcag ctatcgggcc agaaacgccc tcgccctccg
     2221 cctcagcagc cgtaaaagca gcttcaccgt gaccgggacc actttcagcc ctgcccctag
     2281 atcctccact ccccgaacct aatccactac ctccacttga tgacatacca tgatcccctg
     2341 ttcgtttgag cttatggtat cagctcgaag tctatacatc aaagcaggac catccggtcc
     2401 tgcttatttt cgattttaat gcaagctacc cttgggtaga acgcggcttt ttcggcttta
     2461 agcgctcgta acgctcggca aggaactcat acttactgga gatcatgtcc ttggcttctt
     2521 cgagactcat ataaaagact tccggatcct cgtaaggatt ggctttgagg gccttaatgc
     2581 ggcgacctct tgaatgactc atgtttgcac cccttataat tataatgcct tcagtatctt
     2641 ccaaaccacg agtctaatca ttagcgctta ttacaaacaa acgagtgtca tttgtgttag
     2701 ttttggcggt ttgcggtaat gctttaatac tgaatgtaag agcgataaat atagccgttt
     2761 ttaccctggt aagtgactcc ttgccaggtg gtgtcggcat cgtcgcgcag ctgcaccgtg
     2821 gtgccggctt caagggtcgc aataatgggc gtagaaacgc tttttccagc tctcaaattt
     2881 acaaaactat tggtaaacgc ggttttggca gccggtttag cgggggcggc tttcggcgta
     2941 acagtggccg gtgctaccgg aacaggcgct ggtttcgaag gagtggccaa aggcaccgga
     3001 gcggccggct gcaaggttgc tgtttggggc ggtatgccca gggctttttg gatgtctgcg
     3061 atcgaaggtt gagcgacctg catatacgtg atcggtatcg ccacaaccgc aatcactagg
     3121 ctgaggctta agcgatcaag cacaggatga ggaacggtgg ctaacatacc tacctctgat
     3181 agtagctaaa gtagatataa accccgaggg aaataatcaa cccaagcaat ataaggagaa
     3241 ataagaagcg ccaaaatgcc ggccaaaagc gtggcttagc gggaaatgcc gcatcggtat
     3301 accggtcttg ctggggcgat cgttgtcttg gcggcggtgt agcagcttgt ttcgatgatt
     3361 gagccggaga aggcgcagga gttggaggag gcgctgcaac cgggacttga gaccgctccg
     3421 gctgcttctt cttgacaacc gacggcttcg tatcgactgc tgctggcgcg ttaaaggaag
     3481 agcttttttc gcgcaaatca attacgtgtg cgctgccggc tggcctgccg gcaggcctag
     3541 ccgctgaact ccctacgcta tcaagcgttc ggggccggga tggtcgtaat ttgttcggat
     3601 ccattactgc acagtataga ctgagcggtc taattattca acttatgctt tcccgagtaa
     3661 agctgatgag ttcataagaa aacgccccgc tttcgggacg ttttcctggt taatcagcga
     3721 ctgattagcg aggctgttga ggccgggctt cgctcacaat gatgcggcgg ccaccaatct
     3781 ctttttcgtt caaaagcttc acagcgttct gagcttcttc gtcactcgac atttctacga
     3841 aaccaaaacc gcgggaacgg ttggtatcgc gatccatgat gaccttggct gatacaacag
     3901 taccagcttc agcaaaggcg gactcgagtt ccgcatcagt gacgctgtag gctaacgagc
     3961 ctacaaataa tttagttgcc atgcttattc tcccgtttct acgaggtaag ctgacaactg
     4021 aaggtcactt agctgctcct ctacacgaaa cttaattgat tttttaaatc tagacctgag
     4081 gttagttttt acgccaacac tggtagcagc ttctttctag cccccatacg gtaccatgaa
     4141 ttgcttggta tgactagggg aaaattccgc ccgactttga acactttttg tcgccggcgt
     4201 aattaggctg caaggagggc tttactcagt tgacgcaatt ggtgtttacg atgcttctca
     4261 taggcagctt tattactgcc attgtgccgg ataaagctgt tcaaatgctc ggtaccaata
     4321 agatgaggca tcatgacata ggttgcccct ttctcataca gtgcctgggc atcctcatag
     4381 gttgatgcat ggcagataaa gacgctgtcg gtattatggc tgctaatgtg gcttagcagc
     4441 aggctgttgg tttccagact ggtgatgttg ctaataatga gctcggaacg gtgaactcct
     4501 atctcgtcca gcagttcagt atcggtacta tcaccataga tgtgaacaat atgctgatgc
     4561 tgcagaacct cgataatccc cggattataa tcgataacca catagcgctt tttcatggcc
     4621 cggaaggttc tcacgaagtc atgcccccca ttctggtagc ctatcagcac taatggataa
     4681 tgctctaacg cccgtacctc tttcttagtt tcggcccgct caaacaccga cagctgcttt
     4741 tcgaagagcc gatacaactt atgatcatag ttcataaggt aggcagaaac aataatggta
     4801 atcagtgccg ttagcgttac catagcggta atttcggggc gaatggtatc ggttttttga
     4861 gcaagaacca ccaaaataat cgaaaactcg ctgatctggg aaagatgtac cgccgcccta
     4921 aaggcagtct gcttggtgta gccgagcgcc cccagactgg ctatgatgat gagcggcttt
     4981 gtaatgagga tgatggctga aaaaatcagt gccggtacca gcgcagcctg caggttatta
     5041 atgcccatgc ggctgcctag ctcaataaag aagagcagga cgaaaaagtc gcggagcggc
     5101 tttaagcgat tgctaatctc ctgggcatat ggcaagtgag ccattgctac gccggcaaaa
     5161 agcgccccga cttcaattga aaagcctgac agctgaaaca aactggctac accaaagccc
     5221 caggctagag ccaaaatata gagcagctcc tggctttcag cgaaaagctt ggaaaaacgc
     5281 ggcataagat acatgccaat cagagccaaa ctgcctccta ataccatccc ctttacaatg
     5341 agtagccaga aatctcccag cgtgaccgaa ccgccggtac tggttgagac ccataacagc
     5401 gcgacggtag cggcgacatc ttcaaccagg agaataccaa tggccagctg gctgtacaga
     5461 cgcgactgtt ctttacgatc gctcagcgcc tttaccacga taatcgtgct gctgaagagc
     5521 aaagcgactg ccacaatcaa gcactcttgc atggaaaagc ccagtatcgc accggcaccg
     5581 tagctgatgg caccgactcc gaccgtaatt aggagaaacg tcaccagaac cggcttaccg
     5641 gtactcttaa taaccgccgc attaatcccc aacccaatca taaatagcaa aagcactatg
     5701 ccgatctcac taaaggacgc aagaatgtcg tggtcggcgg taatatctaa aaatgtcggg
     5761 ccaaccaaga agccggtaat aatataccca ataatcatcg gctgtttgag cgccttgaaa
     5821 atcaaggaaa ctatcgcagc cagaaccata atgctactca gctgataaaa cagcgactgt
     5881 tccatgcaaa ttccgcttat ggctatatta tgacatagag ggggctgtcg gttccactcc
     5941 tgaaacctca aaaaaccgca gcagcatgat ttttattgct gcaagcgctc gtctatatgc
     6001 atgatcggat cataccaacc ctgatcgagt acaaaggttt gaaactgacg gtccaagcca
     6061 aagctaacag caagtcctac cacaatcagg acaaagccta agctacgcat aaaccacccg
     6121 tccggattac tggcaatcgc caaccgccgc gcaaaggcct ggccggcaat ggaaattaaa
     6181 agcagcatga gcgatagtcc caatgcgtag gcagccaagt acaccactcc ttgagcaaat
     6241 gattgcggca ggatggcagc aacgatcagg
//
LOCUS       DASVGB010000087         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_926523, whole genome shotgun sequence.
ACCESSION   DASVGB010000087 DASVGB010000000
VERSION     DASVGB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926523"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2151
                     /locus_tag="VF272_02650"
     CDS             <1..>2151
                     /locus_tag="VF272_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7259808.1"
                     /translation="IDQLYKKNDILRLYLNEIPYGSQAYGVEAACRTFFPGNIKDDNC
                     SAQLTLSQSAMLAAVANLPTYYSPYGQHIDALKSRQALVLDLMADQKYITDAEADAAK
                     ITDFAAIGLAPAPQIATQVSPYPHFAQYAQEYLEGKYGTRTVESGGLKVITSLDIKKQ
                     KLMQRAINGTNGIAAIRRGGGSNVAMVSADPKTGQVLAMMGSYDPNDKDFGAFNVALA
                     NRQPGSSFKPITYAAAMKGNWGPGSTIYDVKTDFGGGYSPKNYSGTHYGIQSMRSSLA
                     GSLNIPAVKMLHIAGVKNTISTAHDMGISTLNKPAGDYGLSLVLGSGEVKLNDMVNAF
                     ESFANGGVHYEATPILKVTDPRQRVLMDTSKPKKPKRVLDAQISYLVAHMLSDNRARQ
                     YIFGANNPLQVNGRTVAAKTGTTEHYNDAWTMGFSPDIVTGIWAGNNDNRPMSSSAVN
                     IAAPLWHDYMAGYTDRQTREKVEGALDDYPASSNWTAPPGIQTLTIDADTGGAITSAT
                     RSRRTDIFPGWYKVQAAGGIKSANIDKVSGKLATECTPQAAVQNVTAGQIHAEIPTTD
                     PAYPRWQAPIAALAQRLGYASGVGSIPTERDTLHNCSDVKPSVNVSASGTSTLLITAN
                     VTSGTHTANSLTVYFDDQVISTQAINGSTNYSFAYNAPSSGSHTVRAVVTDTALYSGQ
                     DETTVTTTASGGFVGIFPPSPANGSFIPQNTPIT"
BASE COUNT          564 a          618 c          504 g          465 t
ORIGIN      
        1 aattgatcag ctctacaaaa aaaatgacat tctgcgactt tatcttaatg aaatccctta
       61 tggctcccaa gcatacgggg tggaagctgc ctgccgtacc ttcttcccgg gcaatattaa
      121 ggacgataat tgttcggccc aattaacgct gtcccaatca gccatgctag ctgcggtcgc
      181 caatttgccc acctattatt cgccgtacgg ccagcatata gacgcgctca aatctcgcca
      241 agccctggtg ctcgatctga tggccgacca gaaatacata accgatgccg aagctgatgc
      301 ggcaaaaatt accgacttcg ctgccatcgg cctagcaccg gctccccaga ttgcaaccca
      361 ggtgagtcct tacccccact ttgcccagta tgctcaagaa tacttggaag gtaagtatgg
      421 cacgcgaacc gttgaaagcg gcggactcaa ggttataacc agcctcgaca ttaaaaaaca
      481 gaaacttatg cagcgcgcga ttaatggcac caatggaatt gccgctatcc gcaggggcgg
      541 tggctcaaat gtcgccatgg tttcggcaga ccctaaaacc ggccaagtgt tagcaatgat
      601 gggctcttac gacccgaatg ataaagattt tggagcgttt aatgttgccc tggcaaaccg
      661 ccagcctggc tccagcttta aaccgattac gtacgccgcc gccatgaagg gcaattgggg
      721 tcccggcagc acgatttatg atgttaaaac tgattttggc ggcggttatt cgcctaaaaa
      781 ttattcgggt acccactacg gaatccaatc aatgcgatcg tccttagccg gctctctgaa
      841 tattccagcc gtaaaaatgc tgcatatcgc aggtgttaaa aatactataa gtaccgccca
      901 cgacatgggc atcagtacgc tcaataaacc ggctggcgat tatgggctga gcttggtctt
      961 gggctccggc gaggtaaagc tgaatgatat ggttaatgct tttgaaagct ttgctaatgg
     1021 cggcgtgcat tatgaggcca cgcccatcct taaagttacc gacccacgcc aacgagtact
     1081 catggatacc agcaaaccca aaaagcctaa acgagtactt gatgcccaga tctcgtacct
     1141 tgtcgcacac atgctcagcg ataatcgggc gcggcagtac atctttggtg caaacaatcc
     1201 cctgcaggtt aatggccgta ccgttgccgc taaaaccggc actactgaac actacaacga
     1261 tgcctggacc atgggctttt cgcccgatat agtaaccggc atatgggccg gtaataacga
     1321 taaccggccg atgagctcaa gcgcggtcaa catcgccgct ccgctatggc atgactacat
     1381 ggccggctac accgatcgtc aaactcggga aaaggttgag ggcgccttag atgactaccc
     1441 agctagcagt aactggaccg ctcctcccgg tattcaaacc cttaccatcg atgccgatac
     1501 cggcggtgct atcacatccg caacccgtag ccggcgtacc gacatcttcc ccggctggta
     1561 taaagttcaa gcagccggcg gcattaaaag tgccaatatt gataaggtat ccggcaaact
     1621 ggcaaccgaa tgtactccac aggctgcggt gcaaaatgta accgccgggc aaattcatgc
     1681 ggagattcct acaaccgacc cagcctatcc ccgctggcaa gcgccaattg ccgcgctggc
     1741 acagcggctt ggatacgcat cgggagttgg aagcatacct accgaacgcg acactcttca
     1801 caattgtagc gacgttaaac cctcggtaaa cgtcagcgcc agcggcactt ccaccttact
     1861 aataaccgct aatgtaacct cgggaacgca caccgccaac agcttaacgg tatattttga
     1921 tgatcaagtt atttcaaccc aagccattaa tggcagtacc aactacagct tcgcctacaa
     1981 cgccccctct tccggtagcc acactgttag ggctgtagtt accgacaccg cgctgtacag
     2041 cggccaggat gaaactacgg tgactaccac ggcatcagga gggtttgtcg gcatattccc
     2101 accgtcaccg gctaatggaa gcttcattcc gcaaaacacc ccgatcacct t
//
LOCUS       DASVGB010000088         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_13733, whole genome shotgun sequence.
ACCESSION   DASVGB010000088 DASVGB010000000
VERSION     DASVGB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13733"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF272_02655"
     CDS             complement(<1..356)
                     /locus_tag="VF272_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7259809.1"
                     /translation="MAKQQLTIKRFDPELPLPERKTTGSAAFDLSARIDTEIPAGGFK
                     LVPLNVAIALPRGHWGLLAVRSSLHKQGLIPANGVGVIDHDYQGDDDEYHAALYNTTN
                     QPVIVSRGMRVAQLVVV"
     gene            complement(363..1532)
                     /locus_tag="VF272_02660"
     CDS             complement(363..1532)
                     /locus_tag="VF272_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7259810.1"
                     /translation="MERRSRKLGFVVPVLVIALVAFIGGQAAPKIIPPSWVLGHKPLD
                     FESLNDTYDLLERKFDGKLDQTKVIDGARAGVVGSAGDPYTVYQDAKEAKDLRDQLSG
                     TLSGIGAEIGIKNNKLTVISPIAGAPAEKAGLRVGDYIAGIDKKDSSAYTLDEAVAKI
                     RGKGGTKVTLTVVRGSSQPIEITITRAVINVPSVTWSMKEGSIGYIKISTFGDDTTEK
                     MKIAAGELKSQGAKSIILDVRNDPGGYLHVAVDVVSQFVPEGKVVVDERHNGRSQQKL
                     TSEGGGLLEGLPVVVLINGGSASASEIVAGALRDNIKAKLVGEKSFGKGSVQDIVKLE
                     GGAQLKITVAHWFTPSGKGIDRVGIKPDIEVKQTLEDFSAGRDPQLNRALQELKK"
     gene            complement(1553..2584)
                     /locus_tag="VF272_02665"
     CDS             complement(1553..2584)
                     /locus_tag="VF272_02665"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEX7259811.1"
                     /translation="MAPIAHATSLDEQIAELTQQANSQQSQANQLRAQSDTLANKLAS
                     INNQAAALATQMSLNRAKQQRILSQIEDAKAQLVQKKELLDENVRTIYQQSQISPLEM
                     LAASKSLSEYVDRQQYLDRIKDHVQEAAADIQNLKAELEKQQVAVENLINQQSDLQRA
                     LAIQRSEAATILEQTRGEESRYVSMAKANEEQANVLKARQAAILAATFGGSANGGAAC
                     GGGYPGKWCNIPRDTAVDNWGMYNRECVSYTAFRVAASGRYMPYWGGRGNANQWPANA
                     RAAGIPVDGNPRVGDVAVTMAEPYGHVMYVERVNGNGTIFVSQYNYGNNGEYSTMTIS
                     SDDLQFIHF"
     gene            complement(2889..3809)
                     /locus_tag="VF272_02670"
     CDS             complement(2889..3809)
                     /locus_tag="VF272_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX7259812.1"
                     /translation="MLITVLRILKTGLRNFVRNAWLSTAATAVMTVTLSLVAVSYIAN
                     AALTSAIQEVVNKIDISIFLNDANTPQQTQAFKSKLEQAENVKSVKFLTKSDALKEYR
                     RQNADNPKLLEGISETDNPLPASFQIKAEDPNKIDGIIALANQAEFKPMLAPKNPISY
                     SGKRKATIDKIISTSNFLKASGIIASVVFIIISTLIIFNTIRMAIFTRREEIEIMKLV
                     GATSWFIRGPFIFEAILYGVIAAILALIFLYTVVLAGAPRISSYIPEINTIISYMKDH
                     VALVALIELAIGVAIGAGSSLVALKRYLKL"
     gene            complement(3809..>4265)
                     /locus_tag="VF272_02675"
     CDS             complement(3809..>4265)
                     /locus_tag="VF272_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7259813.1"
                     /translation="HLRRRIGVVFQDFKLLQNRNTFENVSFALEAAGASNREIKRSVP
                     RMLQLVGLKNKEASFPRELSGGERQRVAIARALVRQPKILIADEPTGNLDPKTSWDII
                     ELLLKINRFGTTVLLTTHNKEIVNALKRRVITIQRGRIIKDEEQGRYAL"
BASE COUNT         1031 a         1144 c          946 g         1144 t
ORIGIN      
        1 accaccacca actgagccac gcgcatccca cggcttacta tcactggctg attggtagtg
       61 ttatagaggg cagcatggta ctcatcatca tcaccttgat agtcatgatc aataacgcca
      121 acgccattcg ccgggattaa tccctgtttg tgtaacgaac tgcggacagc cagtaaaccc
      181 caatgtccgc gaggcagtgc aatcgccaca ttcagcggca ccagtttaaa gccgccagct
      241 gggatttcgg tgtcaattcg ggctgaaaga tcaaacgctg ctgaaccggt ggttttacgc
      301 tctggcagag gcagttcggg atcgaaacgt tttattgtta gctgctgttt tgccatactc
      361 ggttacttct tcagttcttg caacgcccta ttgagctgag gatcgcgtcc agcgctaaaa
      421 tcttccagcg tttgctttac ttcaatatcc ggcttaatgc ccaccctatc aatccccttg
      481 ccgctgggag taaaccaatg tgccacggta atcttgagct gagctccgcc ttcaagtttg
      541 acaatatcct gcacgctccc tttgccaaaa ctcttttcgc cgacgagctt ggctttaatg
      601 ttatctcgga gagctccggc cacgatctca ctggctgagg cgctgccacc attgatgagc
      661 actacaaccg gcagaccctc aagcagcccg ccaccttcgc tagtcagctt ctgctggcta
      721 cggccgttat gacgctcatc aacaactacc ttgccttccg gaacaaactg gctgaccacg
      781 tcaaccgcta catgcaaata gccgcccggg tcattacgta cgtcaagaat aatgctctta
      841 gctccttggc tttttagctc gccggccgcg attttcattt tctcggtcgt gtcatcgcca
      901 aaggttgaaa tcttaatata gccaatgctt ccttccttca tactccaggt aacgcttggc
      961 acattaataa ccgcacgggt aatggtgatt tcaatcggct gactgctgcc acgcaccacc
     1021 gtaagggtta ccttggtccc tcccttgcct cgaatcttag ccacagcctc atccagcgta
     1081 taagccgagc tgtctttttt atcgattccg gcaatatagt cgccgacccg taaaccggct
     1141 ttttctgccg gagctccggc aatcggcgaa attaccgtaa gtttattatt tttaatgcca
     1201 atttcggcac caataccgga aagcgtgccg ctcaactgat cacgtaaatc tttagcctcc
     1261 ttggcatcct gatagaccgt gtaaggatct ccggctgaac caacaacgcc ggcacgggca
     1321 ccatcaatta ccttcgtctg gtcgagtttc ccatcaaatt tacgttctaa caagtcgtac
     1381 gtatcattta ggctctcaaa gtctaacggc ttatgaccga gtacccagga gggaggaata
     1441 attttaggag cagcctgccc cccaatgaat gcaaccagtg caattactag caccggcaca
     1501 acaaatccta atttacgact gcgtcgctcc aaaactcctc ctcacttcgt ggttaaaaat
     1561 gaataaactg caaatcgtcc gacgatatcg tcatcgttga gtattcaccg ttgttaccgt
     1621 aattatactg gctgacaaaa atagtgccat tgccattaac ccgctcgaca tacataacat
     1681 ggccatacgg ctcagccata gtaaccgcaa catcacctac ccgaggattg ccatctaccg
     1741 gaattccggc agccctggca ttagccggcc attgattggc attaccgcgc ccgccccagt
     1801 acggcatgta gcggccggag gccgcgactc gaaaagccgt gtagctgaca cattcgcggt
     1861 tatacattcc ccagttatca acggccgtgt ccctgggtat attacaccac tttccaggat
     1921 atcctccgcc gcatgcagcc cccccgttag cgctgccgcc aaatgtggca gccagaatag
     1981 cggcctgcct cgcttttagg acattggcct gttcttcatt tgccttagcc attgagacgt
     2041 agcgcgattc ctcaccgcga gtttgctcta aaatagtagc ggcctcgctc cgctgaatcg
     2101 ccagtgcccg ctgcaaatca ctttgctgat taattaagtt ttcaaccgcc acctgctgtt
     2161 tttcgagctc ggcttttagg ttttgaatgt cagctgcagc ttcctgaaca tgatccttaa
     2221 tccgatccaa atactgctgc cgatccacat attcgcttaa gcttttactg gcagccagca
     2281 tttccagcgg agaaatttgt gactgctgat aaattgtccg gacattctcg tcgagaagct
     2341 cctttttttg caccaactgc gctttagcat cttcaatttg agacaaaata cgttgttgct
     2401 tggcgcggtt taagctcatt tgggttgcca gtgccgcagc ctgattatta attgatgcca
     2461 gcttgttggc taacgtatcg ctttgagccc gtaattggtt ggcttgggat tgctgactgt
     2521 tggcctgttg ggttagctcg gcaatctgct catccaagct ggttgcatgg gctatcggcg
     2581 ccataaacag caaagtaacc atggctacac tgatgcgtgt aacaaggctg gtttggcggt
     2641 tatttttcgg gttttggcga gcgatgtgtt ttaccctctt aacacttatg attatatccg
     2701 ttttcgctta tgctagtcaa cccggcgaac agatatatgc cgcctgcgct tgactcaccg
     2761 ccccataggt aacaaacccc ttttgacatt tcctgggcca gcaggcatgc atttagcacg
     2821 tatttaacca aaaaaagcac aattctgcgc ctttttttgg gtattgctca tatggcaatt
     2881 tgctcggact aaagcttcaa ataacgttta agggcaacta agctggatcc ggcgccaatc
     2941 gccacgccaa tcgccagctc tattagagcg acgagggcaa catgatcttt catatagcta
     3001 ataatggtgt tgatttcggg tatataactg ctaattcgcg gtgcaccggc caacaccacg
     3061 gtatacagga atattaaagc cagaatagct gcaataaccc catacagaat tgcctcaaaa
     3121 ataaacggcc cgcggataaa ccagctggtg gcccccacca acttcataat ttcaatttcc
     3181 tcgcgacggg taaatatcgc cattctaatc gtattaaaaa tgatcagcgt ggaaataata
     3241 ataaacacga ctgaagcaat gataccgctg gcctttaaga agtttgaagt gctaataatt
     3301 ttatcgattg tggccttgcg cttgccactg taactaatcg gatttttggg agccagcatc
     3361 ggcttaaact ctgcctggtt ggccaatgca atgatgccat cgattttgtt gggatcttca
     3421 gccttaatct gaaaagaggc cggcaaggga ttgtcggttt cggaaatacc ttcgaggagt
     3481 ttcggattat cggcgttttg acggcggtat tcctttaatg catcactttt cgttaaaaac
     3541 ttcaccgact ttacattttc tgcctgctcc aacttactct taaatgcctg ggtttgctgt
     3601 ggcgtgttgg catcattcaa aaaaattgaa atatcaattt tgttcacaac ttcctggatt
     3661 gccgaagtca atgcggcatt ggcaatatat gataccgcga ccaagctaag cgttaccgtc
     3721 ataacggcgg tggcagcagt ggaaagccag gcgttacgaa caaagttacg cagcccggtt
     3781 tttaaaatgc gtaaaacggt gataagcatt agagagcgta ccttccctgc tcttcatctt
     3841 taataatccg tccgcgctga atagtaatta cgcggcgctt caaagcattc acaatttctt
     3901 tattgtgcgt ggtcagcaag acggtggtcc cgaaccgatt gattttaagc agcaactcaa
     3961 taatgtccca tgaagttttc gggtcaagat tgccggtcgg ctcatcggca attaaaattt
     4021 taggctggcg caccaatgcc cgggcaattg ctacccgctg gcgttcgccc ccggaaagct
     4081 cccgcggaaa actagcctcc ttgtttttca ggccgacgag ttgcagcatc cgaggaacac
     4141 tgcgttttat ttcccggttt gatgctccag cggcctccag tgcaaacgaa acattttcaa
     4201 aggtgttgcg gttttgcagc agcttaaaat cctggaagac cactccgatc cggcgacgga
     4261 gatgc
//
LOCUS       DASVGB010000089         6867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1163151, whole genome shotgun sequence.
ACCESSION   DASVGB010000089 DASVGB010000000
VERSION     DASVGB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163151"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF272_02680"
     CDS             complement(<1..201)
                     /locus_tag="VF272_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259814.1"
                     /translation="MSEKSNQERASLAAKVFVSYLVVAATTGLLYFLFLIRPVVLQVI
                     IAVIVALALEPLVRLLVRKGIGR"
     gene            complement(218..985)
                     /locus_tag="VF272_02685"
     CDS             complement(218..985)
                     /locus_tag="VF272_02685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7259815.1"
                     /translation="MSTRFKLLILDADGTSTAPDRASLPSKAVIDAVREAQKSVHVAL
                     GTGRTFRGAQPLIKTLGLRGPSVLTGGAEIVDVSSGAILHNQLLSAKTLREVAAIALR
                     FHHHVFTAGAEPSMPIDAPEQIKHSAAMLVVEGVNDDQIVNIMEEVEAVNGVAAHKAN
                     SWSGEHLADLHITHEQASKRHGAERLIELLKVSKNEVMAIGDSHNDLPLLQAAGFKVA
                     MGGAPDELKAAADYVAPSFEEDGAAEAIRRFILADQG"
     gene            1006..1536
                     /locus_tag="VF272_02690"
     CDS             1006..1536
                     /locus_tag="VF272_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7259816.1"
                     /translation="MNMLDVSLLELVHSVSNPTLDRLAVFLTQLGGAIFITALVSVTA
                     AGLLWQRRRQAATLLVVTIGGAGMLNIMLKLLFQRDRPTLWEALAIEHTYSFPSGHAM
                     ASSALAFALIILVWHTKWRLVALLCGAFYITIIGLTRMYLGVHFPSDIIAGWIVSLGW
                     TYLVYRAVYGLKPHKP"
     gene            complement(1533..1949)
                     /locus_tag="VF272_02695"
     CDS             complement(1533..1949)
                     /locus_tag="VF272_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX7259817.1"
                     /translation="MQEELFQRVQQAGFRLTRARKEIVATFTEATRPLSAIELRLMLS
                     SMPNKSTIYRELLFLMQQGIVVEVQLRPDITHYELAGQVHHHHLVCNRCDGVEDVVLG
                     DDLHQLEAQIAIGKRFKVERHSLEFYGLCGKCQTAG"
     gene            2020..2751
                     /locus_tag="VF272_02700"
     CDS             2020..2751
                     /locus_tag="VF272_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7259818.1"
                     /translation="MDLAYSLIAAISISILALIGLFFAPKNWTHKREIKLLGFAAGVL
                     LATATLELLPEAVEHSQGKWAYYSILAGIVGFFFLERIFRSFHTHRKDQHLKAAGSLV
                     IIGDGVHNFIDGMAIAVAFQVSPMVGVITTLAIAAHELPQEIADYIVLIRSGYSRKRA
                     LLLNFASGLTAITGVLLVFALGGIIESIEGPLLGITVGFFLYIASADIIPELNHKHEA
                     GEKFLLAFILGIALIAGLVLAIPHE"
     gene            2770..3192
                     /locus_tag="VF272_02705"
     CDS             2770..3192
                     /locus_tag="VF272_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX7259819.1"
                     /translation="MQHYPSPALDLVVLVKGEHLFECLERYAASVKYPSAWLSGIGAA
                     SSATIGAYDPAIKGYHWETFKEPLEILSLQGNLSWVDGKPFWHIHGSFAGHNYQSIGG
                     HIKALEISVTAELTITPHPTSLTRTFDEETGIKLLRKQ"
     gene            complement(3203..3547)
                     /locus_tag="VF272_02710"
     CDS             complement(3203..3547)
                     /locus_tag="VF272_02710"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7259820.1"
                     /translation="MSVIDEYLNNVSASHKTELERIRSIIHKTVPEAEEVISYGIPAF
                     KYHKKYLIGFAAFKTHMSVFPGSEPVAAVKEKLANFRLSKGTIQFTKEHPIPEPILKE
                     IIEHSRKRITTS"
     gene            complement(3604..4437)
                     /locus_tag="VF272_02715"
     CDS             complement(3604..4437)
                     /locus_tag="VF272_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX7259821.1"
                     /translation="MEKKLEQHLNKVFARYGEFPARDDVVQELLANLTERYQDFKSQG
                     KRDNQAYRATIDSIGDVSEIMEQIPHSEVKLTSESALHSAVSSMRDMIGANIGSRKFK
                     HSVLTKADLSETNLTKADFTGSALAEASFRGSFLSGAKLRSAALKGANFFNADLTNAD
                     LSGSDLRDACFDSSILTGTKLKGSALHGASFKNANIVGTDFSQSDLSKISFDGQTLEG
                     AVFGSASLKQTSFRNATLRNVSFHHAAVKQAIFDGAAMNKVTYAILKGANATLDNVTI
                     L"
     gene            complement(4450..4779)
                     /locus_tag="VF272_02720"
     CDS             complement(4450..4779)
                     /locus_tag="VF272_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013057038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX7259822.1"
                     /translation="MIDKKISSDLLRGHTDTIILGLLASGDKYGYEITRLVYEKSDRA
                     YELKEATMYSSLKRLENERHITAYWGDETQGGRRKYYCITLTGRKLYEVNKRNWDYAK
                     QILNHLI"
     gene            complement(4885..5385)
                     /locus_tag="VF272_02725"
     CDS             complement(4885..5385)
                     /locus_tag="VF272_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259823.1"
                     /translation="MNEFADDIQYAALAEVLREKFGEDSDMYRDGIAAFLAGEKSGEI
                     PEPYRKYWEPYKTNNLLHHITIEKNIEVIRETGLEPRNPHPRPWTAMKAIFMGEPGDP
                     LFAKSEQAVIKHVADKGDNPVLLKIRTKNKLYKSVEPKRTFQVISLDPIAPEEVVEYL
                     PLHLQK"
     gene            complement(5420..5496)
                     /locus_tag="VF272_02730"
     tRNA            complement(5420..5496)
                     /locus_tag="VF272_02730"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5460..5462),aa:Lys,seq:ctt)
     gene            complement(5509..5584)
                     /locus_tag="VF272_02735"
     tRNA            complement(5509..5584)
                     /locus_tag="VF272_02735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5549..5551),aa:Arg,seq:tcg)
     gene            5834..6223
                     /locus_tag="VF272_02740"
     CDS             5834..6223
                     /locus_tag="VF272_02740"
                     /inference="COORDINATES: protein motif:HMM:NF039825.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HEX7259824.1"
                     /translation="MWKIFTKAGQPGWAVIIPFYNVYVLLKIIDRPGWWLILYIIPFV
                     NIIVSFINALDLAKAFGKGPMFGIFGNFLFTFVGYAILGFGSAQYHGPAGGAAPSQPM
                     PPTTPSVTPPAPPAMPTPPTASTPPTV"
     gene            complement(6257..6333)
                     /locus_tag="VF272_02745"
     tRNA            complement(6257..6333)
                     /locus_tag="VF272_02745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6297..6299),aa:Arg,seq:ccg)
     gene            complement(6346..>6867)
                     /locus_tag="VF272_02750"
     CDS             complement(6346..>6867)
                     /locus_tag="VF272_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259825.1"
                     /translation="QGVFLFSLLAALVFAVGLPLLALAIADKKQLSIAQLFTFDKRWL
                     YMVATYLLIIVPIFLGLLLFIVPGIILAIMWGLAFYIIADKVVTPIEALKESARLTKG
                     YRTRIFLLGLVVAVLAIISSILAILPFIGFIAASMLSTIFAGMYAVVYRKLQVVKGAG
                     SAPSAGAPAVASV"
BASE COUNT         1738 a         1743 c         1580 g         1806 t
ORIGIN      
        1 acgcccgatg cctttgcgga ccaaaagcct aaccaggggc tcgagggcaa gggcaacaat
       61 tacggcaatt attacctgga gtaccaccgg acgaattaaa aacagaaaat agagcagccc
      121 ggtggtcgcg gcaactacca agtaggagac gaatactttt gcggctaggc tggcgcgttc
      181 ttgattgctt ttttctgaca tagtggcatt atacccgtta tccctggtct gccaagataa
      241 aacgtcggat cgcttcagca gctccatctt cttcgaatga cggcgctaca tagtcggcgg
      301 cggctttgag ctcgtccggc gcaccgccca tagcgacttt gaaaccggcc gcctgcagca
      361 atggcaggtc gttgtggctg tcaccgattg ccattacctc gttttttgaa accttcagga
      421 gctcaatcag ccgctcggcg ccgtgccgct tggaagcctg ttcatgagtt atgtgaagat
      481 cggctaaatg ctcaccgctc caggagttcg ccttatgcgc cgccactcca ttcacagcct
      541 caacctcttc catgatattc acaatttgat catcatttac gccttcaact acgagcatag
      601 ctgccgaatg tttgatctgc tcaggagcat caataggcat gctgggttcc gctcctgctg
      661 taaaaacatg gtgatgaaaa cgcagagcaa tcgctgccac ctccctcagt gttttggcag
      721 atagaagttg gttgtgaagt atcgcaccgc tagagacatc gacaatctct gcgcctccgg
      781 ttaacacgct aggaccgcgt aagccaagag tcttgatcaa cggctgggca cccctaaagg
      841 tgcggccggt tcccagggca acatgcaccg atttttgagc ctcccggacc gcatcaatca
      901 cagccttaga gggtaatgat gctctgtcgg gagctgtgga agttccatct gcgtcaagaa
      961 ttaaaagctt aaatcgggta gacataactc aagtatactc gttatatgaa tatgcttgat
     1021 gtttcattat tagagcttgt acattcggtc agcaacccaa cgcttgaccg cctagccgtt
     1081 tttctgaccc agcttggggg agcgattttc ataaccgcgc tcgttagcgt taccgcggct
     1141 ggcctgttgt ggcagcgccg ccgccaggcc gccacgctgc tcgttgtaac catcggcggc
     1201 gccggcatgc taaatatcat gctaaaacta ctattccaac gcgaccgccc tacgctctgg
     1261 gaggctttag caattgagca cacctactcg ttccccagcg gccatgcgat ggcaagcagc
     1321 gccctggcat tcgccttgat aattcttgta tggcacacca aatggaggct tgttgctctg
     1381 ctatgcggtg ccttctacat cactattatt ggacttaccc ggatgtattt gggcgtccat
     1441 ttcccatcag acattatcgc cggatggatt gtaagcctgg gatggaccta tttggtatat
     1501 cgcgcggtat acggcttaaa accgcataag ccttagccgg cggtttggca cttcccgcat
     1561 aatccataga attccagact atgtcgctcc actttaaagc gcttaccaat ggcaatttgc
     1621 gcctccagct gatgcaagtc gtcgcccagc acaacatcct ccacaccatc acaccgattg
     1681 cacactaaat gatgatggtg tacctggccg gcgagctcat aatgggttat atccgggcgt
     1741 agctgcactt ctacaacaat gccttgttgc atcaaaaaaa gcagctcgcg ataaatcgta
     1801 gacttgttgg gcattgaact aagcattaac cgaagctcaa tagctgacag cgggcgggtg
     1861 gcttcagtga aagtcgctac aatctctttg cgtgcccgcg taagccgaaa accagcttgc
     1921 tggacccttt ggaatagttc ttcttgcatg aaattaattg tagcgctatg atatgtctat
     1981 tgcaaccaag ttgcattaac aacacggagg aatgatcaat tggatttggc gtactcgctc
     2041 attgcagcca tcagcattag cattcttgct ctaattgggc tgttcttcgc gcccaagaat
     2101 tggacgcata aacgtgaaat taagctgctc gggtttgccg ccggagtact gctcgccacc
     2161 gcaaccctgg aactcttgcc tgaggcggtg gaacatagtc aagggaaatg ggcgtactac
     2221 tcaattttag ccgggattgt gggatttttc tttcttgaaa gaattttcag gtcctttcat
     2281 acccaccgca aagaccaaca tctgaaagcg gccggttcgc tggtgataat tggcgatggc
     2341 gtgcataact ttattgatgg tatggctatc gccgtagcct ttcaggtcag ccccatggtg
     2401 ggcgttataa ccacgctcgc catagcagct catgagctgc ctcaagaaat tgccgattac
     2461 atcgtgttaa tacgcagcgg atatagccga aaacgtgcat tattgctgaa ttttgcctcg
     2521 ggccttaccg cgataacggg tgttttgcta gtgtttgccc taggaggcat tatcgaatcg
     2581 attgaaggac cgttactggg aattacggtt ggcttcttcc tctacattgc ttccgccgac
     2641 atcatacccg agcttaacca taaacatgaa gccggcgaaa agttcttgct ggcgttcatc
     2701 cttggcatag ccttgattgc gggactggtg cttgccatcc cccacgagta attagctata
     2761 atgaaaagca tgcagcatta cccaagcccc gcacttgatc tagttgtcct tgtcaaagga
     2821 gagcatcttt ttgaatgtct agaacggtat gccgcatcgg taaagtaccc aagcgcttgg
     2881 ttaagcggta ttggcgcagc ctcaagtgca acaatcggtg cctatgatcc ggcgatcaaa
     2941 ggctatcatt gggaaacctt taaagaacca ttggagattc tcagcctgca gggcaatctc
     3001 agttgggttg acgggaagcc gttctggcat atccatggct catttgctgg acataattac
     3061 cagtccatcg gcggccacat taaagcgttg gagattagcg taaccgccga acttaccatc
     3121 accccccacc ccacatcgct gactcggact ttcgatgagg aaaccggaat caagctatta
     3181 cgtaagcagt agccgccaaa cgctaggaag ttgtaatccg cttgcgactg tgttcgataa
     3241 tttctttaag gattggctcg gggattgggt gctctttggt aaattgaatc gttccttttg
     3301 ataggcggaa attagccagc ttctccttta cggctgcaac cggttccgaa ccgggaaata
     3361 cgctcatatg agttttgaat gctgcgaatc cgattaaata tttcttgtgg tatttaaatg
     3421 ccggaatacc gtaactaata acttcctcgg cttccggaac agttttatga ataatactgc
     3481 gtattctctc aagctctgtc ttgtgcgagg cgctcacatt attgagatat tcatcaatta
     3541 ccgacatggt tttattctag caaacaaacc tacattaaat gtttaccgag ctaaaatact
     3601 gttttacaga atggttacat tatcaagcgt tgcattcgct ccctttaaga tggcataagt
     3661 aaccttattc atagcagccc cgtcaaaaat ggcctgttta acggcagcat ggtgaaagga
     3721 gacattccgc aatgtagcgt tgcggaatga tgtctgcttc agtgatgcac tgccaaatac
     3781 cgcgccctca agggtttgac catcgaacga gatttttgag agatcggact ggctaaaatc
     3841 ggtaccaacg atattagcat tcttaaagct agcgccatga agcgcggagc ctttcagctt
     3901 agtaccggta aggatggagc tatcaaagca tgcatctctc agatcgctac cgctcagatc
     3961 ggcgtttgtg aggtcggcgt tgaaaaagtt agcgccttta agcgcggctg atctaagctt
     4021 agcaccggaa aggaaactac ccctaaagga tgcttcggca agggcgctgc cggtaaagtc
     4081 ggccttcgta agattcgtct cactcaagtc ggctttcgta agcacggaat gcttaaattt
     4141 tctgctgccg atattggccc caatcatatc cctcatactg ctcaccgcac tatgcaacgc
     4201 ggattcggac gttagcttta cttctgaatg aggaatttgc tccataattt cggacacatc
     4261 accaattgaa tcgatagtcg ctcggtaggc ttggttatcc cttttaccct ggcttttaaa
     4321 gtcttggtat ctttctgtaa gattggccag tagctcctgc actacatcat ctcgagccgg
     4381 aaactctcca taacgcgcga atactttgtt taaatgctgc tccaatttct tttccattat
     4441 ggtacctttc tatatcaaat gatttaggat ctgtttggca taatcccaat tgcgtttatt
     4501 aacctcatag agcttacggc cagtcaatgt aatacaatag tatttccttc gaccgccctg
     4561 ggtttcgtcg ccccagtagg cagtaatgtg ccgttcgttt tcaaggcgct taagacttga
     4621 gtacatggtg gcctctttta actcgtacgc gcgatccgac ttctcgtaga cgagcctggt
     4681 tatttcgtag ccgtatttgt caccgctcgc tagcaacccc aggataattg tgtcagtgtg
     4741 gccgcgaagg aggtccgaac ttattttctt atctatcatt gttaaaatgt atagcacact
     4801 actatgtctg tcaaggtaat taaaatgacc aaaagagtta ttatgcataa ttgcattgga
     4861 gggtcatcgt tcgtactatc gcaactattt ttgcagatga agcggcaaat attccacaac
     4921 ttcttccggt gcaatcgggt cgagtgaaat gacttgaaac gtacgcttcg gctctaccga
     4981 cttatacaac ttattctttg tacgtatttt aagtagcacc ggattatcgc ctttatccgc
     5041 aacatgctta attactgcct gctcagactt agcgaataat gggtcgccag gctcccccat
     5101 aaagattgct ttcatcgccg tccacggacg aggatgtgga tttcgtggtt cgaggccggt
     5161 ttcacgaatg acctcgatgt tcttctcaat cgttatgtga tgtagtaaat tattcgtctt
     5221 gtatggctcc cagtatttgc gatagggctc agggatttca ccgctctttt cgccagccaa
     5281 aaaagccgca atgccatcac gatacatgtc gctgtcttcg ccaaactttt cgcgcaaaac
     5341 ctcagcaaga gctgcgtatt gaatgtcgtc agcaaattca ttcataccgt aagtataaaa
     5401 cagattttga gataagtact ggagggccat ctgggactcg aacccaggac acgctgctta
     5461 agaggcagct gctctaacca actgagctaa tggcccacaa gggtatattg gtgcgcccgg
     5521 agagattcga actcccgacc ccttggtccg aagccaagtg ctctatccgc tgagctacgg
     5581 gcgcccgtac cgggataata ctagcaaaaa aggcttggtt cggctagcgt ctacccctta
     5641 cattaccttc agaattgcca aaagcgctta tgctatatat actccagata gtacatgctt
     5701 taatcgagga gctacgcaat tggataccgc atatagtgtc tcggcccaat ctacgaattc
     5761 aggaggaaac tcagtattca cactcatact agcgttaatt ttcctgggaa tactcatctt
     5821 ctccatcgca tcgatgtgga aaattttcac caaagccgga cagccgggct gggcagtgat
     5881 tattcctttt tataatgtct atgtattgct aaaaatcatt gaccgcccgg gatggtggct
     5941 tatcctttat attattccgt ttgtaaatat catagtctca ttcattaacg cgcttgacct
     6001 tgccaaagcc tttggcaagg ggccaatgtt cggtattttt ggcaacttcc tctttacatt
     6061 cgtaggctat gcaatacttg gctttggcag tgctcagtac catgggccgg ccggaggcgc
     6121 agccccctcg caacccatgc ctccgactac accatcggta acacccccag cccctccggc
     6181 aatgccgaca cctccaaccg cctcaacacc gcctaccgtt taggttaggc tccccaaaaa
     6241 aactcattat aatacctggt gcgcccgaag ggattcgaac ccccggcctt tggtaccgga
     6301 aaccaacgct ctatccagct gagctacggg cgcaagcctc tggtattata ccgaggcgac
     6361 ggcaggagca ccagctgaag gagcggaacc agcgcctttc acgacttgaa gttttcggta
     6421 aacaacggca tacattccgg caaatattgt cgagagcatg ctcgcggcta taaatccaat
     6481 aaaaggaagg atggccaata tactcgagat aatagctaga acagcgacca ctaatccgag
     6541 caggaaaatg cgcgttcgat atcctttagt aagccgagcg ctctccttca atgcctcaat
     6601 tggcgtcacg actttatcgg caataatata aaatgctagt ccccacataa tagccagaat
     6661 aatcccggga acaataaaga gaagtaggcc taggaaaatc ggaacaataa ttaacaaata
     6721 ggtggcaacc atgtaaagcc atcgcttatc gaatgtaaac agctgagcaa tagacagttg
     6781 ttttttgtca gcaatagcaa gtgcaagcag cggtaagcct accgcaaata ctaaagctgc
     6841 aagcaggcta aaaagaaaaa caccctg
//
LOCUS       DASVGB010000090         4503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_146878, whole genome shotgun sequence.
ACCESSION   DASVGB010000090 DASVGB010000000
VERSION     DASVGB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146878"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /gene="atpD"
                     /locus_tag="VF272_02755"
     CDS             complement(<1..923)
                     /gene="atpD"
                     /locus_tag="VF272_02755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7259826.1"
                     /translation="MATTATEKIPPKAGTTGSIVQILGVVVDVDFDTKLPAIYNALEI
                     EQEGKPPIVLEVAQHLSGRTVRAISLASTDGLYRGQPVKDTAAPISIPVGDDTLGRMF
                     NVTGQPIDGKAGTFTRKASIHREAPLLTEQSGRQEIFETGIKVIDLIAPVSKGGKVGL
                     FGGAGVGKTVIIQELIRNIAAEHGGKSVFAGVGERTREGNDLYYEMKESGVLDKTALV
                     FGQMNEPPGARLRVALSGLTLAEAFRDEGQDVLLFVDNIFRFTQAGSEVSALLGRLPS
                     AVGYQPNLAQEMGQLQERITSTTTGSITSVQA"
     gene            complement(933..1889)
                     /gene="atpG"
                     /locus_tag="VF272_02760"
     CDS             complement(933..1889)
                     /gene="atpG"
                     /locus_tag="VF272_02760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX7259827.1"
                     /translation="MAQMQELQRRVRSISNTRQTTKAMQLVDATKLRRAQAASKRSRD
                     YIEATMATLQRLLSQDMDQSDLTLWKRDKVRSVAIMVIASDRGLAGAYNHNVANRLRL
                     QTARYEEKGVEVKVVAIGAQAIRVAKHLKHATILEQIVGRSTDPGINDVKPLAEFLRN
                     QYVEGTIDAIEVIYTHSISALKQEIRVLPIWPVDLEEAGGARGGDPDSAQAQPSTSGV
                     PTATGPAVSMHPHVITLLEPSAEEVVDVALERLFDASVLNAAQEAGVSEHAMRMLAMR
                     NATDNASDLIDSLTLQINTIRQAGITQEISEITGAAAAIEEQ"
     gene            complement(1889..3424)
                     /gene="atpA"
                     /locus_tag="VF272_02765"
     CDS             complement(1889..3424)
                     /gene="atpA"
                     /locus_tag="VF272_02765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7259828.1"
                     /translation="MAKLTISEITAELKTAISNLPSNAELKGHGIVSRLGDGVATIQG
                     LPQAKLGEVLELKQGGETASVLVLNLLEDEIGAVLLDENDRIGAGAEVTQTGRQLTVP
                     VGDGLLGRVIDPLGRPLDGLGEVKAKETRPIESPAPDVMSRKSVHEPVATGILAIDGM
                     VPIGRGQRQLIIGDRQTGKTAIALDTMLNQMKQGSGIISVYVAIGQKRSTIARLVERL
                     RQADALDSSIIVATSATDPVTLQYLAPYAGASIGEYIRDNGGHALAIYDDLTRHAQAY
                     RQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMSDEKGAGSLTALPIIETQAGNISA
                     YIPTNVISITDGQIFLTTDLFNQGIRPAIDVGNSVSRVGGAAQPKAIKKLAGPLRVSL
                     AQYNEVAAFTQFASDLDEATRAQLDRGQRLVELLKQPQYHPLSVAEQSILLLAGTTGP
                     LDHVKVEDMAEARASLLKHLAKHGKIAESINKTGAITDEERKDLEKIIEDALADVTIA
                     EAA"
     gene            complement(3417..3809)
                     /locus_tag="VF272_02770"
     CDS             complement(3417..3809)
                     /locus_tag="VF272_02770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX7259829.1"
                     /translation="MAKVSYRRLASAMVELLETHERSEVAAAVAAVMTASGSRLHLDR
                     LLPELERELARTSGHIVVHAQTARSLPNEVLERLCAELIKQLKGTSYELTTTVDPQLV
                     GGARLTTADASLDLTMAAQLQQLRISHG"
     gene            complement(3802..4344)
                     /gene="atpF"
                     /locus_tag="VF272_02775"
     CDS             complement(3802..4344)
                     /gene="atpF"
                     /locus_tag="VF272_02775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX7259830.1"
                     /translation="MPIPVLFAAETHSSGLGALGISVPALLFEIINFAILFWILKRYA
                     YKPILNALERRRQTIENSLKAAAEAQAEKAAISKQRAEMLAEAKAHAADIISDARAEA
                     ETKKNAIISEAESTAVHIVEQAKRETGRQLEAARKELATEARKLVIAATSRLIGEKLD
                     EKRDAELLEKAFETAGGRRG"
     gene            complement(4348..4503)
                     /locus_tag="VF272_02780"
     CDS             complement(4348..4503)
                     /locus_tag="VF272_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX7259831.1"
                     /translation="MGLIGAKAIEAVGRNPETQGRVTSLMILTIAFTESLAIFALVFG
                     FIIKFLG"
BASE COUNT         1002 a         1223 c         1181 g         1097 t
ORIGIN      
        1 gcttgaaccg aagtaatcga gccggtagtg gttgaggtaa tccgctcttg gagctggccc
       61 atttcctgag caaggttagg ctgataacca accgcactgg gcaaccggcc gagcagtgca
      121 ctgacttcgg agccggcttg ggtaaagcgg aagatgttat caacgaacag gagcacatcc
      181 tgcccttcgt cgcggaaggc ttctgccaaa gtgagaccgg aaagcgcaac cctcaagcgg
      241 gcacccggcg gttcattcat ctggccaaat accagagctg ttttgtcgag cacaccggac
      301 tctttcattt cataatacag gtcgtttcct tcacgggtac gctccccgac tccggcgaaa
      361 accgacttgc cgccatgctc agccgcgatg ttgcgaataa gttcctgaat gatgaccgtc
      421 ttgccaacac cggcgccgcc gaacaaccca accttgccgc ctttggaaac cggcgcaatc
      481 aaatcgataa ccttgatacc ggtttcaaaa atttcttgac gtccggattg ctcggtaagc
      541 aagggtgctt cgcggtggat ggaggctttg cgggtaaagg tgccggcttt accgtcaatt
      601 ggttggccgg ttacgttaaa catccgaccc aatgtgtcat cgccgactgg aatactaatc
      661 ggcgcagcgg tatctttcac cggttgcccg cgatataaac catcggtaga ggccaacgag
      721 atggcacgca ccgttcggcc gctgagatgc tgagccactt caagtacgat cggaggcttt
      781 ccttcttgct cgatttccaa agcgttataa atagccggca gcttggtatc aaaatcgaca
      841 tcaaccacaa cgcctaaaat ctggacgatg cttccggttg ttccagcttt aggcgggatt
      901 ttttcagtag cggtagtagc cataatgttc tcctattgtt cctcaattgc ggcagcggca
      961 ccggtaattt ccgagatttc ctgggtgatt ccggcctgcc ggatagtgtt aatttgtaag
     1021 gtaagcgaat caatcaagtc gctggcgtta tcggtagcat tgcgcatcgc cagcatccgc
     1081 atggcatgct cgctgacacc ggcttcctga gcggcattca aaaccgatgc atcgaataag
     1141 cgttccagcg ccacatccac cacctcctcg gcacttggct ccagtaaggt aatgacgtgg
     1201 ggatgcatgg acacggcggg tcctgtcgct gtcggcaccc cgcttgtgct cggctgcgcc
     1261 tgcgcgctat cggggtcccc gcctcgtgcg ccacccgcct cttctaaatc aaccggccag
     1321 attggtaaaa cccgaatttc ttgctttagc gctgaaatcg agtgcgtata aataacttcg
     1381 atagcgtcaa tagtgccttc aacatattga ttgcgcagaa attcggccag cggcttaacg
     1441 tcattaatac cggggtcggt tgaacggcca acaatttgtt ccagaatagt agcgtgcttt
     1501 aaatgcttag ccacgcggat tgcttgagcg ccgatggcca ctactttaac ttcaacccct
     1561 ttttcctcat aacgggccgt ttgcaggcgc agacggttgg caacattgtg attataagca
     1621 ccggcaaggc ccctatctga agcaatgacc ataattgcaa cgctgcggac cttatctcgc
     1681 ttccataagg ttaagtcact ctgatccatg tcttggctca gtaaccgctg aagcgtggcc
     1741 atcgtagctt caatgtaatc acgcgaccgc tttgaagctg cttgggcgcg gcgcagtttg
     1801 gtggcgtcca ccagctgcat cgccttcgtg gtttggcggg tgttgctgat tgagcgaaca
     1861 cgcctctgta gctcttgcat ctgagccatt aggcggcctc ggctatggtt acatcggcaa
     1921 gagcatcttc tataattttc tctaggtctt tgcgctcttc gtcggtaata gcgccggttt
     1981 tgttgatcga ttcggcgatt tttccatgct tggccagatg cttgagcagg cttgcccggg
     2041 cttcggccat atcttcaacc ttaacgtgat caagcgggcc ggtagtgcct gcaagcaaca
     2101 agatgctttg ctcggcaacg ctcagtgggt ggtactgcgg ctgctttaat agctcaacta
     2161 aacgctgccc gcgatcgagc tgggcacggg tagcttcatc cagatcacta gcgaactggg
     2221 taaacgccgc aacctcatta tattgagcca ggcttactcg cagcgggccg gctagttttt
     2281 taattgcctt gggctgggct gccccgccga cgcgactaac cgagttacca acatcgatag
     2341 ccgggcgaat accttggtta aataggtcgg tagtaaggaa gatttgacca tcggtaattg
     2401 aaataacgtt ggttgggata taagcggaaa tattaccggc ttgggtctca ataatcggca
     2461 gggccgttaa tgaaccggcg cctttttcat cactcatttt agcggcgcgc tcaagcaaac
     2521 gtgaatgcag gtagaaaaca tctcccgggt aagcttcgcg gcccggaggt cggcgaagca
     2581 acagcgacat ctgccgatag gcttgagcgt gacgggttaa gtcatcataa attgccagtg
     2641 catggccgcc attatcgcga atgtactcgc caatagaggc accggcgtag ggagcgagat
     2701 attgcagcgt tacgggatcg gttgccgaag tcgccacaat aatgcttgaa tccaaagcat
     2761 cggcttggcg caggcgttca accaagcggg caatcgtgct gcgtttttga ccgatggcca
     2821 catacaccga gataattccg cttccctgct tcatctggtt gagcatagta tcaagggcaa
     2881 tagcggtttt gccggtttgg cggtcgccaa taatcagctg gcgctgtccg cgaccaattg
     2941 ggaccatgcc gtcaatcgct aaaataccgg tggcaaccgg ctcatgcacc gacttacggc
     3001 tcatgacatc cggcgccgga ctttcaatag ggcgggtttc cttggccttg acctcgccta
     3061 gcccatcaag cggacgcccc agcgggtcga tcacgcgtcc aagcaggcca tcgccaaccg
     3121 gcacggtaag ctggcggccg gtttgggtga cctcggcgcc agctccaatc ctgtcatttt
     3181 catccaaaag aacggcaccg atttcatctt caagtaagtt caataccaaa acactggcag
     3241 tttctccgcc ctgctttagc tccagaactt caccaagttt agcctgcggc agaccttgaa
     3301 tggttgcaac accatcgcct aagcgcgaga caatgccatg acccttcagc tcggcattac
     3361 taggtaaatt agaaatagcg gttttcagct cagcggtaat ttcggaaata gtcagtttag
     3421 ccatgagata tccttaattg ttgtaattgg gcagccattg taagatcaag cgacgcatcg
     3481 gcggtggtta gccgtgcgcc tcccactagt tgaggatcga ccgtagtagt gagttcatag
     3541 ctggttccct ttaattgctt tataagttcg gcgcataatc gctcaagaac ttcattcggc
     3601 agcgaacgcg ccgtctgagc atgcaccaca atatgtccgc tggtgcgtgc cagctcacgc
     3661 tcaagctcgg gtaagagccg gtccaggtga agtcgagatc cgcttgcagt cattactgcg
     3721 gcgaccgcag cagcaacctc actacgttca tgtgtctcga gcaactctac catagcgctg
     3781 gctagccggc gataacttac tttagccacg acggcctccc gcagtttcaa aggctttttc
     3841 caaaagctca gcgtcgcgct tttcatccaa cttttctcca atcaacctgg atgtcgcagc
     3901 aatcaccagc ttgcgtgcct cagttgccaa ttccttgcgc gcagcttcga gctggcggcc
     3961 ggtttcgcgt ttggcctgct ccacaatgtg aactgcggta ctttcagctt cggaaataat
     4021 tgcgtttttc ttggtttcgg cttcggctct ggcgtcggaa ataatatcgg ctgcatgggc
     4081 tttagcctct gccagcattt cagcgcgctg cttggaaatg gcagcctttt cagcctgggc
     4141 ctctgctgct gcctttaagc tgttttcgat ggtttggcgg cggcgctcaa gcgcgtttaa
     4201 aatcggcttg taggcgtacc gcttgagaat ccaaaatagg attgcaaagt tgataatctc
     4261 gaagagcagc gccggcacgc taatgccaag cgcacctaag cccgagctat gagtttctgc
     4321 ggcaaacaat accggaatcg gcataggtta tccgagaaac ttaattatga atccgaatac
     4381 gagggcgaaa atagccagtg attcggtaaa ggcgatggtt agaatcatta ggctggtaac
     4441 gcgtccctgg gtttcggggt tgcggcctac ggcctcgatt gccttagccc caatcagacc
     4501 aat
//
LOCUS       DASVGB010000091         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_869905, whole genome shotgun sequence.
ACCESSION   DASVGB010000091 DASVGB010000000
VERSION     DASVGB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869905"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF272_02785"
     CDS             complement(<1..407)
                     /locus_tag="VF272_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017377637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7259832.1"
                     /translation="MELLNLILHFDEHLRTAIEQLGSFSYLLLFAIIFAETGLVVTPI
                     LPGDSLLFAAGTLAGVGMLNIWILVPALIAAAVLGDTVNYWVGHRSGEAVFRGKNSRI
                     FKPAYLHKTHAFFEKYGGKTIIIARFVPIVRTFA"
     gene            598..858
                     /locus_tag="VF272_02790"
     CDS             598..858
                     /locus_tag="VF272_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259833.1"
                     /translation="MTEEINEPIKVLAGFDYTKRASVVLPYLIDWRGHRYKVTQFGIH
                     YPARRGKKFYHVFELLCDTAKFKLELDTETLSWSLAEVYYDH"
     gene            848..2119
                     /locus_tag="VF272_02795"
     CDS             848..2119
                     /locus_tag="VF272_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259834.1"
                     /translation="MTIEPNRAAPKVMHIDLNSAFAMTEQQANPLLRGRPVGVTNRLN
                     DYAICITASNEAKNLGIGIGTRWHEARAIAPGFTMLESDPDKYTHVHRKMKAIFESYS
                     PTAYMKSVDEGVIDFQGMRSILKGRSLEDIAQEIKQRVRVEVGDYMTVNIGIAQNRWL
                     AKVAAEFHKPNGLLTINPGEQEALFCMLPLTKLPYIKRRTERRLNRVGIYSAVDFYHA
                     PYWLLFRQVFGSVNGHHWYLKLRGYETERQYGIRTVGRNYVLEHRTDNVEELSLLFHK
                     AAVKISRRLKRNNLAARGLSLYLRYDQPRGSDTHYDHPRRWHGRQMWSTAVRRGDQLH
                     QRAMELFRSGPPGLTVNHLYMISYGLEPYRGTVQPTLFNDSQAQRERLEDAINTINDR
                     YGELIIQPATVALSKNPMKDKIPFGTIRYFE"
     gene            2164..2520
                     /locus_tag="VF272_02800"
     CDS             2164..2520
                     /locus_tag="VF272_02800"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX7259835.1"
                     /translation="MQNKVFLNPEGFLEIVFVGHQTGETFQDVYEKVLPLLAGMKAEG
                     TPLRGLFDLTLQTGYSLNSDKAAMKFLEETDYDRVAMYHVPHAEVTKAIILAMGKSDN
                     TKLFESREEALAWLMA"
     gene            2520..2972
                     /locus_tag="VF272_02805"
     CDS             2520..2972
                     /locus_tag="VF272_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX7259836.1"
                     /translation="MDRKTLNKTAVSTTLHCLTGCGTGEILGLVIGEVLGLGVGITII
                     LAVGLAFVFGYALTTLPLSRAGLSWRAAFRIALAVDTLSIAVMEIVDNAAMLLVPGAL
                     NTGLANPLFWISLAFALVAAFFAALPVNRYLLTKGKGHALIHKYHDRH"
     gene            complement(2977..>3762)
                     /locus_tag="VF272_02810"
     CDS             complement(2977..>3762)
                     /locus_tag="VF272_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7259837.1"
                     /translation="STYVKSLGFKDAKDAEGETAMVGVTNGFGKQTVIKGTIVGVLKP
                     GIIDAGGVTVNRSFNQAVYAFQTEGVPPQVRQQFQMVTARFDVNMASEELVKLKEEIK
                     KKGYDGQTFEDRIGTFKQVVDGIIMALNLFGAIALLAASFGIINTLLMAVQERTKEIG
                     LMKAMGMRGQRIFLLFTGEAVMLGFWGSLLGVLAAIGIGNVINHVAITSFLKGLVGLE
                     LLSFPLQSVAGIMAIVIGIAFLAGTLPALRAARQNPIDSLRYE"
BASE COUNT          964 a         1015 c          917 g          866 t
ORIGIN      
        1 gcaaaggtcc taacaattgg tacgaagcgt gcaatgatga tggtttttcc tccgtatttt
       61 tcaaaaaaag catgggtttt gtgtaaataa gctggcttaa aaatccgaga attcttaccc
      121 ctaaagaccg cttcccccga tctgtgcccc acccagtaat taacggtgtc gcccaatacc
      181 gcagctgcga tgagggcggg aaccagaatc caaatattta acattccgac tccagccagc
      241 gtaccggccg cgaaaagcag cgagtcgcct gggaggattg gagttacaac taagccggtt
      301 tcagcgaaaa taatagcaaa cagcagcaaa taactgaacg atcctagctg ctcaattgcc
      361 gtccgaagat gttcatcaaa atgcaaaatc aggttgagca gttccatggg ctaagtatag
      421 cgtactttta ttaaagtaaa gtgattgcta aatgtggcac aaaattagca caaatcatac
      481 acacaacagg cacagaatag tgccgcattt gtgcctcctc cataaagtaa aagtggtctc
      541 gatactacag acttgtgttc gctatttgtt cggtatacta tggaggcagg ggatgttatg
      601 accgaagaaa tcaatgaacc cataaaggtc ttagctggat tcgactacac caagcgcgcc
      661 agcgtcgtcc tgccctatct gatagattgg cgcggacacc gttataaggt cacccagttt
      721 ggtatccact atccagcgcg gcgcggtaaa aagttttacc acgtctttga gctgctctgc
      781 gataccgcca agtttaagct ggaactggac accgaaactc tcagctggag cttagctgag
      841 gtgtactatg accattgagc ccaaccgagc cgcaccaaaa gttatgcaca tcgacctgaa
      901 cagcgccttt gccatgaccg aacaacaggc caacccgttg ctccgcggcc ggccggtggg
      961 ggtcaccaat cggctcaacg actacgccat ttgcattacc gcttccaatg aggctaaaaa
     1021 tcttggtatt ggcatcggta cccgttggca cgaggcgcgg gcaatcgcgc ctggctttac
     1081 catgctggaa agcgacccgg ataaatacac tcatgtgcac cgtaagatga aggctatttt
     1141 tgaaagctac tccccaaccg cctatatgaa aagcgttgat gaaggcgtta ttgattttca
     1201 gggcatgcgc tccatactca aggggcgaag tctggaagat attgcccagg agatcaaaca
     1261 acgggttaga gtagaggttg gcgattacat gacggtgaac attggcattg cccagaaccg
     1321 ctggctggcc aaggtggcag ctgagtttca caagccgaac ggcctgctca ccattaaccc
     1381 cggtgagcaa gaggctttat tttgtatgct gccactcacc aagttgccct acatcaagcg
     1441 gcggacggaa cggcggctca atagagtggg aatttatagc gcggtagatt tctatcatgc
     1501 cccttattgg ctactattcc gccaggtatt tggcagcgtc aacggtcatc actggtattt
     1561 gaaattgcgc ggctacgaaa ccgaacgcca gtacggcatc cgcaccgtcg gccgtaatta
     1621 tgtactagag caccggaccg ataatgtaga ggagttgtcg ctgctctttc ataaagcggc
     1681 tgttaaaatc agccgccgcc taaaacgtaa taacttggcc gctcgaggct taagtcttta
     1741 cttgcgttac gatcaaccgc gcggcagcga tacccactac gaccatcccc ggcgttggca
     1801 cggacgccag atgtggtcta ccgctgttcg gcgcggcgat cagctgcacc aacgcgccat
     1861 ggagctcttc cgctccgggc ctccaggact aaccgtcaac cacctgtata tgatcagtta
     1921 cggcctcgag ccctaccggg gcactgttca gccaacccta tttaacgatt cccaagctca
     1981 acgggagcgg ctggaagatg ccattaatac tatcaatgac cgatacggcg aattaattat
     2041 tcagccggct acggtagccc tgtccaaaaa cccgatgaag gacaaaatac cctttggcac
     2101 catccgctat tttgaataat gctaggcatt tacaaaaaag tttaggtgag ccatgatgaa
     2161 gccatgcaaa acaaggtttt tctcaatccc gaaggctttt tagagatagt ctttgtcggc
     2221 catcagaccg gcgagacctt tcaagacgtc tacgagaaag ttctaccctt actggcggga
     2281 atgaaggccg aaggcacacc gctgcgggga ctgttcgacc tcaccttgca aaccggctat
     2341 tcactcaatt ccgataaagc cgccatgaaa tttctggaag aaaccgatta cgaccgggtc
     2401 gcgatgtacc acgtgcccca cgccgaagtt accaaggcta tcatcctagc aatgggaaaa
     2461 agcgataata ccaagctctt cgagagccga gaggaagcgt tagcctggct gatggcctga
     2521 tggaccgtaa aacccttaat aaaaccgccg taagcaccac cctgcactgc ctaaccggct
     2581 gtggcactgg tgaaatcctg gggttggtga taggcgaagt cctgggcctg ggtgtcggaa
     2641 tcaccattat cttggctgtg gggctggctt ttgtcttcgg ctatgcgctc acaacacttc
     2701 cactcagtcg ggctgggcta tcttggcgcg cggcatttag gatcgctctt gccgtcgaca
     2761 cgctttcaat tgccgttatg gagatcgtcg acaacgcggc catgctcctg gtgccagggg
     2821 cattaaacac cgggctcgca aatccgcttt tctggatcag tttggccttt gccttagtgg
     2881 ctgcattttt tgctgctctt ccagttaacc gctacttgct taccaaaggc aaaggacacg
     2941 cgctaataca caagtaccat gaccgccatt aatgcgctac tcgtaacgca gagagtcaat
     3001 cgggttttgg cgagcagccc gtaacgccgg cagagtgccg gccaagaaag caatgccgat
     3061 gacaatggcc atgattccgg caaccgactg caaggggaag gacagtagct ccaggcctac
     3121 cagacctttc aggaagctgg ttatggcaac atgattaatg acattgccaa taccaatcgc
     3181 ggccaacacg cctagaaggc tgccccagaa accgagcatg accgcttcac cggtaaagag
     3241 caggaagatg cgctgccccc tcatgcccat ggccttcatc aagccgattt ctttggtgcg
     3301 ctcctgtacc gccatcagta aggtgttgat aattccaaag ctagcggcta gcaaagcgat
     3361 cgcaccgaaa agattgagcg ccatgataat cccgtcaact acctgcttga acgtaccgat
     3421 ccgatcttca aaagtctggc catcatagcc ttttttctta atttcttctt ttagcttgac
     3481 caactcttcc gatgccatat tcacatcaaa ccgggcggtc accatctgga actgctggcg
     3541 cacttggggc gggactccct cggtttggaa tgcgtacacc gcttgattga atgaacggtt
     3601 taccgtcaca ccgccagcgt caataatccc aggtttaagt acaccaacga ttgtcccttt
     3661 aattacggtc tgcttgccaa acccattggt taccccaacc attgccgtct cgccttcggc
     3721 atctttggcg tccttgaaac caagactctt gacgtaggtt ga
//
LOCUS       DASVGB010000092         5486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_18699, whole genome shotgun sequence.
ACCESSION   DASVGB010000092 DASVGB010000000
VERSION     DASVGB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18699"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VF272_02815"
     CDS             complement(<1..205)
                     /locus_tag="VF272_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259838.1"
                     /translation="MKPLRAADTATQALLFVGTAHFLSQGFTQAKNSRKPVNSLAIAY
                     MGLLSAAALYQAGRLIGPKTREEK"
     gene            complement(291..1295)
                     /locus_tag="VF272_02820"
     CDS             complement(291..1295)
                     /locus_tag="VF272_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7259839.1"
                     /translation="MAEATKIPITALTGFLGSGKTTLLNYILEHNHGLKIGVVVNDYG
                     SVNIDAQLIAGKTDTMLELTNGCICCSMEGLELDEAIGQFAYPGTPIDYIVIEASGLA
                     EPADLARTLREAAGRFTMLDSIVAVIDAMNAEENAEGHATALQQIEYSDFVIINKIDL
                     VSAAKVEQLSELARGINPRVRIFTAVKGVVDVRLLLDQGRYAAPPTQLSSNGHAGHKH
                     LHDHYATFSYATNKPLSPQAFQSFVNLHIPTSVYRAKGIVNLGNKGRRRKYIFQLVGK
                     RAELTWDDWGAQQPSTDLVFIGRDFNEDAIRKLLEACIDDKPNEHDPAQEVVLPKRQR
                     "
     gene            1370..1753
                     /locus_tag="VF272_02825"
     CDS             1370..1753
                     /locus_tag="VF272_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7259840.1"
                     /translation="MTSVLLVEDDNDIRAAYNYALSKAGFAVTEASDGAKAAEYIEKL
                     RPDVVLLDMLMPGMSGLDFLRQTEVASRFPDTKVVAVSNIDTPRVVQQAKELGVTEYI
                     IKVETTPRELVELVGKLAKVEDDAH"
     gene            1781..2356
                     /locus_tag="VF272_02830"
     CDS             1781..2356
                     /locus_tag="VF272_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259841.1"
                     /translation="MSYPLHDFRIENENLILLSFANGTTARVQPNDLAEYLSKPDLAR
                     VTKAMSARRKFIKRVLPPTAVVLLSASILALGAHDARQFMNHLQSPQPTAKEQPLSAQ
                     PEPAQATSTQQSARLPATQYRATSTKPNPVAPSVAAPSSSATAKPTLGMPGSNSKSSG
                     IASGAQNTPSGSSRQLIQSIPHNLKSLLKPD"
     gene            2416..2679
                     /locus_tag="VF272_02835"
     CDS             2416..2679
                     /locus_tag="VF272_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX7259842.1"
                     /translation="MSSVQHDITVYTTEHCVYCHVQKDFLQEHNISFREVRVDSDTEA
                     AEEMITRSGQMGVPFTIITNQAAETEDEIVGFDRLRLTKVLGI"
     gene            2681..3640
                     /locus_tag="VF272_02840"
     CDS             2681..3640
                     /locus_tag="VF272_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7259843.1"
                     /translation="MTTVYDIVIVGAGPTGLAAAIYTSREDLSTLVIDGGAVGGMIAT
                     TEVVDNYPGFPEGIGGLALADAMKKQAERFGSEVKTGIWANSLKRTKQHIELETGKGI
                     IKARSVLIATGSLYRHLDVPGEKELEGKGVHYCATCDGPLYRDAHLIAVGGGNTMLQE
                     GLFLTKFVKKLTVLVRKPEFKGSEILINQLQTHDNVEVVFNTSVSEVVGEGPMFTSVK
                     TINNQTKAAGEIKGDGLFVFAGLLPNTDWLKGEVELDERGFVHVNHAFATNIPGVYAA
                     GDVIVGSVGQVASAVGEGVSAALSIRQFLDPNHALPSYAVNQV"
     gene            3843..3911
                     /locus_tag="VF272_02845"
     tRNA            3843..3911
                     /locus_tag="VF272_02845"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            3925..4000
                     /locus_tag="VF272_02850"
     tRNA            3925..4000
                     /locus_tag="VF272_02850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3961..3963,aa:Val,seq:cac)
     gene            4197..4496
                     /locus_tag="VF272_02855"
     CDS             4197..4496
                     /locus_tag="VF272_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259844.1"
                     /translation="MSPGARERLLKAYAICGSEMRIIALEKRKTAVRVRPTDRAALER
                     LGQARGFEVMDHPSFDIAKEAAMREDIDKRSASKPKSRRRRSTRSAGEHYPSLAW"
     gene            4664..4897
                     /locus_tag="VF272_02860"
     CDS             4664..4897
                     /locus_tag="VF272_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259845.1"
                     /translation="MSLTNEDLKQIKTLVTDSVEGAEKRLKTSITSEIQASEQRMTRA
                     IVGHSDARYEQLSVQMENGFTHLQNQFRKSIRP"
     gene            4936..5463
                     /locus_tag="VF272_02865"
     CDS             4936..5463
                     /locus_tag="VF272_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7259846.1"
                     /translation="MAELSVGKDAPKVVNLVIEIRRGEGKNKYELDKKTGYLVLDRVN
                     ATMLKYPCDYGYVPNTLCEDGDPLDGLLVIDESVPQNIVVPSRAIGVLYMVDDGEGDE
                     KLICVADDDVTMQHIQEVEDLGGYFKPMVEQFYSHYKDWKNDWKGVEVHFNGWGNADA
                     ARKVILESIERHTKS"
BASE COUNT         1370 a         1310 c         1447 g         1359 t
ORIGIN      
        1 gcttttcttc ccgtgttttt ggtccaatca atcgcccggc ttggtaaagg gctgcggcgc
       61 taagtaggcc catgtaggcg atggcaaggc tgtttaccgg cttgcgagaa tttttggctt
      121 gagtaaatcc ttggctaaga aagtgtgctg ttcccacaaa caataacgct tgtgtggcag
      181 tatcggcggc tcgaagtggt ttcatagttg atcctccctt gaataaatta tgcgttcagt
      241 atggaggatg ctgagcgcga tgagtatgta agatattaca aaaaaatgcc ttagcgctgc
      301 cttttgggca aaacaacctc ctgcgcaggg tcatgttcgt tcggcttatc atcaatacag
      361 gcttccaata gctttcgtat cgcgtcctca ttaaaatctc ttccgataaa tacgagatct
      421 gtagagggtt gttgagcacc ccaatcatcc cacgtcagtt cggcgcgttt tccgacaagc
      481 tgaaaaatgt acttgcggcg gcggccctta ttgcctagat tgacaattcc tttagcccga
      541 taaacagaag tcggaatgtg gagattaaca aagctttgaa aagcctgcgg gctaagcggc
      601 ttattggttg cgtaggagaa ggtggcgtag tgatcgtgaa ggtgcttatg cccagcatgg
      661 ccgtttgagc tcagctgggt tggaggggcg gcatagcgcc cttggtcgag caatagacgt
      721 acgtcaacta ctccctttac cgccgtaaaa atacgtacgc gcggattaat gccccgtgca
      781 agttcgctaa gctgctctac cttggccgcc gataccagat ctatcttatt aataatgacg
      841 aagtcgctgt attcaatttg ttggagcgcg gtagcatgtc cctcggcatt ctcttcggca
      901 ttcatggcgt caattaccgc aacaatgcta tcaagcatag taaatcgacc ggcggcttcc
      961 cgcaacgttc gtgctaaatc ggctggttcg gcgagaccgc tagcctctat gacaatgtag
     1021 tctatcgggg ttccggggta agcaaactgc ccgatagcct catccagctc taacccttcc
     1081 atggagcagc agatgcaacc gtttgtcagc tccaacatgg tgtcggtttt tccggcaatg
     1141 agctgggcat caatattgac agaaccataa tcgttgacga ctacgccaat ctttagtcca
     1201 tggttatgtt ctaaaatata attgagcagc gttgtcttac cggagccaag aaaaccggtt
     1261 aaggccgtaa tagggatttt tgttgcctct gccatctaat cagtatagcg ggtaggctct
     1321 tgttgtaata ttgcttatgc tctaaactgc tatatattag ggggctccca tgacaagcgt
     1381 tttactggtt gaagatgata atgatattcg agcggcctat aactacgcct taagtaaggc
     1441 cgggtttgca gtaaccgagg ctagcgacgg cgccaaagct gctgagtata ttgaaaagct
     1501 tcgtcctgat gtggtgctgc tagatatgct catgccgggc atgagcggac tcgatttttt
     1561 acgccaaacc gaagtagcga gccgatttcc ggataccaag gttgtagcgg ttagtaatat
     1621 tgatactccc agagtggtgc agcaagccaa ggaacttggg gttaccgagt acattattaa
     1681 ggtcgaaacc actccacgcg agttagtcga attggttgga aagttggcca aagtcgagga
     1741 tgatgcccac taagcttagc tagtttgata atatggttat atgagctatc cacttcacga
     1801 ctttcgcatc gaaaatgaga acctaatttt gctttcattt gccaatggta cgactgcgcg
     1861 cgtacaacct aacgatttgg ctgaatacct atcaaagccg gacctggcga gagttaccaa
     1921 ggccatgagc gcccggcgca aatttattaa gcgggtcttg ccgccaacgg ctgtagttct
     1981 gctctcggct agtattttag ctcttggtgc tcatgatgcc cgacagttta tgaatcattt
     2041 gcaatcgccg caaccaacgg cgaaggaaca gccattgtca gcccagcctg agcctgctca
     2101 ggctacatcg acccaacaaa gtgcgaggtt gccagctaca cagtaccgag cgacatcaac
     2161 gaagccaaat ccggtggctc caagtgtggc tgcgccttct tcatcagcta cggccaaacc
     2221 gacactgggc atgcctggtt ctaattccaa aagctcaggg attgcgtccg gtgcgcaaaa
     2281 cactcctagt ggttccagcc gccaattgat tcaatcaatt ccgcacaatt tgaaatcttt
     2341 gctaaaacca gattagcttc tgcgctaagc taagattctg tatactaaag taaactagag
     2401 gggctaccgc caaccgtgtc ttccgttcag catgatatta ctgtttatac taccgagcat
     2461 tgtgtatact gccatgtcca aaaagacttt ttacaggagc acaacatatc ttttcgtgaa
     2521 gtcagagtag atagcgatac ggaggctgcg gaggaaatga ttacacgcag tggtcaaatg
     2581 ggcgtgccat ttacgattat taccaatcaa gcagctgaaa ccgaagacga gattgtcggc
     2641 ttcgatcggc tacggttaac gaaagtcctg ggaatctaat atgactacgg tgtatgacat
     2701 tgtaattgtg ggtgccggtc ctaccgggct tgccgccgcg atttatactt cgcgcgagga
     2761 cttgtccaca ttggtaattg acggcggtgc ggtcggcggt atgattgcca cgaccgaagt
     2821 ggtggataac tatcctggat ttcctgaagg cattggcggt ctagcgttgg ccgacgccat
     2881 gaaaaaacag gctgaacggt tcggttcgga agtaaaaacc ggcatttggg ccaatagttt
     2941 aaagcgcaca aagcagcata ttgaacttga aaccggtaaa ggaattataa aagcccgcag
     3001 cgttttgatt gccaccgggt cgctgtatcg gcatctggat gttccaggtg aaaaagaatt
     3061 ggaaggcaag ggggtgcatt attgtgctac ctgcgacggt ccgctctacc gagacgccca
     3121 cttaatagcc gtcggcggtg gcaataccat gctgcaagaa ggattgtttc ttaccaagtt
     3181 tgttaaaaag ttgacggtac tggtgcgcaa accggagttt aaaggttcgg aaatcttgat
     3241 caatcaactc caaactcacg acaatgttga ggttgttttt aataccagcg tgagcgaggt
     3301 ggttggcgaa ggtccgatgt tcacctcggt aaaaaccatc aataatcaga ccaaagcagc
     3361 tggagagatc aagggcgacg gcttatttgt gtttgctggt ctgcttccta acaccgattg
     3421 gctgaaaggc gaggtagagc ttgacgagcg tggtttcgtg catgtgaatc atgccttcgc
     3481 taccaatatt cccggtgttt atgcggctgg cgatgtaatc gttggctcgg ttgggcaagt
     3541 agcttcagcg gtaggcgagg gagtttcggc ggcactttct attcggcaat tcctagaccc
     3601 gaaccacgca ttgccaagct atgccgtgaa tcaggtatag tctttttctc caagaactat
     3661 ccccattttt tagttgacat gcatattcgc tcgatgtaac gtaagaagca tgaagtctca
     3721 gtctaaccac agcacaatta tttaccgttg gtttccggta gtactgctgt ttggtcggta
     3781 ctgaggctcg ttgtaactta cacaaaagtg cgaccggcct cccagaggcc ggtctttttt
     3841 tacggggtgt agctcagctt gggagagcgt cgggaaaccg gaggccgcag gttcaagtcc
     3901 tgtcaccgcg actttgaaaa acttggcgag atcgcttacg tggtaagtaa gcgccaacta
     3961 caccagttgg taatttacgg gttcgaatcc cgtctcgcct atgcagcacc atcacaacca
     4021 ccacaacgca ggaccgtcgc tggaggcgac catctatgcc aaccgctcaa cgcgcagtgc
     4081 ctggtcataa gtctcggaat gcaggtctga agcacaccgt cgcggtcctc atctgtccga
     4141 ctcgcgacct gagctcgtct gcagttgacg cgctcgccga gctgcttccg ctcggtatgt
     4201 cgccgggggc cagggagcgg ctgctcaagg cctatgccat ctgcgggtct gagatgcgca
     4261 tcatcgcgct ggagaagcgg aagaccgccg tacgggttcg cccgaccgat cgagctgctc
     4321 tggagcggct cggccaggcg cgcggcttcg aggtcatgga ccacccgagc ttcgacatcg
     4381 ccaaggaggc cgccatgcgc gaggacatcg acaagcgctc ggcatcgaag cccaagtccc
     4441 gccggagaag gagcactcgc agcgccggcg agcactaccc cagtctcgcc tggtaggcac
     4501 ctggtgggtg tgatgtatca agtgtagggc acaacccttt acgcctgatc ggcgtcccgg
     4561 ttgcggttcc acaagaatcg caccggggcg ctgcccacgc tatcatcaca gtttaatggt
     4621 ttgttcgcta ggcaaggctg tagaagttta ttatcataag catatgagcc tgacgaacga
     4681 agatttaaaa cagatcaaaa cgcttgtgac tgattcggta gaaggtgcgg aaaagcgctt
     4741 gaagacaagc attacctctg aaatccaggc ttcggagcag cggatgactc gagccattgt
     4801 cggccatagc gatgccaggt acgaacagct ttccgttcaa atggaaaatg gtttcacaca
     4861 tctgcaaaat cagttccgca aatcgatccg accctagtct gctatactga aaatcaacac
     4921 taataaggaa gcagcatggc agaattaagt gtcggtaaag acgctcctaa ggttgttaat
     4981 ttagttattg aaatccgccg cggcgagggt aaaaacaagt acgaacttga taaaaaaact
     5041 ggctacctgg tactcgaccg ggttaacgct accatgctga aatatccttg cgattatggc
     5101 tatgtgccga acacgctgtg cgaagacgga gacccgctcg atggtctctt agtaattgat
     5161 gaatcagtgc cccagaatat cgtggtgccg tctcgtgcta tcggtgtttt atatatggtg
     5221 gatgacggcg agggcgacga aaaattgatc tgcgtcgccg atgatgacgt taccatgcaa
     5281 cacattcaag aagttgaaga tctaggtggt tactttaagc cgatggttga gcagttttat
     5341 tctcattata aagactggaa gaatgactgg aagggcgtgg aggtgcattt caatggctgg
     5401 ggaaacgccg atgcggcgcg taaggttata ttggagtcaa ttgagcgcca caccaagagt
     5461 taacaggagc ttgcatcaag aagcta
//
LOCUS       DASVGB010000093        13342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_521852, whole genome shotgun sequence.
ACCESSION   DASVGB010000093 DASVGB010000000
VERSION     DASVGB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521852"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2421)
                     /locus_tag="VF272_02870"
     CDS             complement(<1..2421)
                     /locus_tag="VF272_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7259847.1"
                     /translation="MATVFALALVGAVSGGNQILASHSAAMCSSSNFPYGFLCPNDDT
                     ADLANNVKLTPIDGTPGGSTDIDAPQQVGPGATFRYETGYDSYQPSGGTNGRNVYNWV
                     ILDIRDALFDYTQGIRLYGTTQGPSNDIQYNDGTAADTPMPAACGGDPNSAAPGCRPG
                     PGSPGDVFNACPLEDFAVGPYSGINNDPPEPTGWVGCGLQGKSIVWMRNQDAGNNHIR
                     YRFKFSLPDYVSTDTVPSDSASKFCLRNHVSLGEDQPAASISASNDMRRIARLGDMRC
                     YFLSPTTLSGSVTSDDEEATGRALPGVQLTFSNCEGGDIKAGASERTNSSGNYDVRAV
                     IGARVCITAPNEYTGPDGTQYVLRNPITQGNGEADGGYLHQVPGMNCRSDGPGNLGNP
                     NFYPACNRSDVQLYDRTADDKYDFVYVPVPIPPPMIKGATPGNGVPVVLGQRIDYTFT
                     VQNNRDGNYSNVNISDNIPLNMKQGLSDVFVDSVQFINSGSPPPGWSSETFFYTGPGA
                     DNLCRKDGSVFNIPIGYGYSKNFYTEFSCNVHAATTAAPAHIALEFLEMPAYSTLIIK
                     WHGTVKSDAADIGVYPAGATVDSFCANGSATDFTNPGWLSQCEKPDRSEGLQGVVNYT
                     YASVDNSFTGWSNRVFHPIIGRVAPPVKSVTIGGKGLPECDLDGDGVCEYDPANIKYD
                     NGSYKIEVVPDTTAGPIEYYVLDATNNNYADLGADPLNPVIQSSENNCIPDFQRITCS
                     STVAWGAIRWPTDNMPHPAHFIINVKFGGNEPIGSRFSNVAKLCWIKYWEDAHGVECL
                     PSNPATVER"
     gene            complement(2556..3080)
                     /locus_tag="VF272_02875"
     CDS             complement(2556..3080)
                     /locus_tag="VF272_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259848.1"
                     /translation="MKFVRAVLAAAFAAACMTAVTPASGSSVMSAYAQHSGADCKSNA
                     YRVQYNAGGKANTGDLGRLRLTKRMLPGTSYVTVTARPRRGAVLCVWVEAHKGSLESI
                     RCRPYVGPDRRLRPWGKEMLLGFYGSQVRLRPGDYVRSNGFRLTWLRGGECQLRYRDP
                     AQMRSMTLTGRKRL"
     gene            3285..4397
                     /locus_tag="VF272_02880"
     CDS             3285..4397
                     /locus_tag="VF272_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259849.1"
                     /translation="MKLSLPVNFPTKASLQSWPAKNYFILGGVLLLLLLAAGGIYAAF
                     LPPTSTIKVQTYEVVNGTPQRLGNVTVQLDSYKSSAECADSTKTTEPASTGANHGIVN
                     FYGCPTGIRYTIANMSRSGYVQAANSPIKTKTSRFYSRKNRVTTINLYLVKAGDAAPI
                     RISGPSKKNNIYNTASRDHFYNKVNPDGAWIMNFTDTYYIGMVYDGWSIDVAKKSEAG
                     WYFGLVSRPDGTVIGCAWIEPEIVRPGTDTPSANSCSGQDATLKDRDRVGMDFNCAFN
                     TDGSCNRPASAVEVGPNCDTTLYKNYFTNNNYESGSFADPVGKIESFPTNFDPPQRAG
                     YRFTTHDEKAAVVFSSTYGWGFIDRTCIPGTPRGTN"
     gene            complement(4463..4864)
                     /locus_tag="VF272_02885"
     CDS             complement(4463..4864)
                     /locus_tag="VF272_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259850.1"
                     /translation="MKCRFTPVVLTLVAALAAAAPAAASQPEVISAKLSPQGSSSPAK
                     VVLVVNHSAMKVISFGLRDSFLSQDRPRGLNSPKPTKLMNNNWRFVVSLPVGLKPGEY
                     IFCWRLNANTKNGCKAEGYAQQLVFQMRLAR"
     gene            5171..5731
                     /locus_tag="VF272_02890"
     CDS             5171..5731
                     /locus_tag="VF272_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259851.1"
                     /translation="MLSVMAARAAAFDYKGNIEVKVIDFNSGTYLPITTAVVSVKVIG
                     AATNCPSPTQTTDANGRAIFVGCPAYSTGVKKYKLVDVSAPGYVVRPNSNYKKATSGF
                     YLFGKGRKKTRIRVYMQRAQAAQAPRPTRPPSNANDPWGASPNDNTPVACRAVLAPGI
                     APLAWATDAGYYANDAKGFNRMMDVL"
     gene            5746..7188
                     /locus_tag="VF272_02895"
     CDS             5746..7188
                     /locus_tag="VF272_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7259852.1"
                     /translation="MSQKRIILAAIAITTIVVIAGGIYLAISYKNPQSPAETVYSDPD
                     LQDITSVNGKRLQEITLPNGKKTKVRAEVLMVRITDPNTVKTLGGRATPFPGIPQVYE
                     VKVPVGKDLMQAKAELEKMPGVAYAEPDYINFAQALPRIIPNDTHIANQASLNDISAP
                     EAWALNTGSSGTTIAIIDSGAWGTHPDLVNKYSINAADPVDGVDNDGNGYVDDNFGMD
                     FVRGTIRDANGDGTVEWVNDANGADDDNHGGLNGDGHGTAVAGAAAAQSNNGAGVASV
                     NWNARILPIAVGSDGGGDITYFESHVAAGIYYSIARGAQVISLSIGRLCAAETTCPSE
                     LMRQSMQAAQASNVVTVAATGNNSTSNEIHNIARIATIAVAAIDTGTLTKASYSNSGP
                     EVDIGAPVPVVTTQKGGGYVPMAGTSLATPQVAAAAAYVLGLNPGLSPAQVLNVLQAT
                     ANAPPGGRNNDVGAGRINLYKLIRFCQTCI"
     gene            complement(7256..7723)
                     /locus_tag="VF272_02900"
     CDS             complement(7256..7723)
                     /locus_tag="VF272_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259853.1"
                     /translation="MRAATLAVLAAVVLLVPGSANASVAKTAGSAVGSATLSYDIPAP
                     VTMVHGKVTRTYNSRHCRPLAVRIWFERKVAGGTWQRFDPEHVTKSARGNTSRISAWI
                     TDFPPSLGTLYSINSVSPVVCPGKLRTLRMRLVIEGLTNSVAPSGSPQGMFGS"
     gene            7896..8954
                     /gene="ychF"
                     /locus_tag="VF272_02905"
     CDS             7896..8954
                     /gene="ychF"
                     /locus_tag="VF272_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7259854.1"
                     /translation="MKIALTGLPNVGKSTLFNALTKNNVLAANYPFATIEPNVGVVPV
                     PDERLAVLARLYDAKKIVPATVTFVDIAGLVKGASEGQGLGNQFLANIRECDAICQVV
                     RAFSDPNVQHVSEKVDPLDDITTINTELILADLQTADKRLSKLKKDASQKALLAELTK
                     ARAILDKGELLGSHPELDREVLKELQFMTLKPFVFAFNVDEDQLSNTERQKELTALVS
                     PAPCVFLCAKVEAELSTLPEADAAELLHELGQAEPGLVALAREGQRALQLQSYLTAGP
                     QEVRAWTIPIGATAPQAAGVIHTDFEKGFIKAEVVSYEDLVGAGSLVAARGAGKARLE
                     GKDYVMKADDVVEFRFNT"
     gene            complement(9083..9823)
                     /locus_tag="VF272_02910"
     CDS             complement(9083..9823)
                     /locus_tag="VF272_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX7259855.1"
                     /translation="MSAKNKQRLDLLLVERGLAPTRTAAQAMIMAGEVMSNTTKLVKP
                     GLLVETDAEIEVADRPRYVSRGGDKLASVAEALKLDFNDKVVLDVGSSTGGFSDYALQ
                     HGAAKIYAVDVGTAQLAYQLRQDVRVIIMEKTDIRTVQAEQLDPLPDRAVIDVSFVSL
                     TKILPTVAQLITPAGQIIAMAKPQFEADKPTADRYKGVIKDEKVRTNILAELEAVMAE
                     QFTITASADSAVHGAQGNRERFYVLTLI"
     gene            complement(9807..10370)
                     /gene="efp"
                     /locus_tag="VF272_02915"
     CDS             complement(9807..10370)
                     /gene="efp"
                     /locus_tag="VF272_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7259856.1"
                     /translation="MIYGHTELRKGVVIDLDGVPHQVIDSSHLALGRGGAVMRTKLKN
                     LITGASFDKSFRSSDKVPAADIAKRTMQFLYNDDDSLHFMNQETYEQDQINRSLAPDA
                     TSYLKEGSTAIVMYFDEKPIGVDIGNNVFLQVSSTEPGVKGDTATAALKPATLETGLE
                     VMVPLFVNEGDVIKVDTRDGKYLERQK"
     gene            complement(10386..10604)
                     /gene="rpsR"
                     /locus_tag="VF272_02920"
     CDS             complement(10386..10604)
                     /gene="rpsR"
                     /locus_tag="VF272_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7259857.1"
                     /translation="MANFYSKKVCRFCAEKLDDVDYKDVKLLQRYVNSYGKIESRKRT
                     GTCLKHQRRVAIALKRARHLAFLPFVVS"
     gene            complement(10617..11081)
                     /gene="ssb"
                     /locus_tag="VF272_02925"
     CDS             complement(10617..11081)
                     /gene="ssb"
                     /locus_tag="VF272_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7259858.1"
                     /translation="MAKSFNQAIVMGNLTRDPELRTTPGGQPVASFAVATNRSWVDGG
                     GERKEAVEYHEIVAWGKLGELTSQYLAKGRKVMVVGRLQTQSWEKDGIKRQRTEIVAS
                     DINFLDRPGEAGSDHPAESSSDAPAKPAAKKKDVVIEDLKDDAPVNLDEIPF"
     gene            complement(11084..11446)
                     /gene="rpsF"
                     /locus_tag="VF272_02930"
     CDS             complement(11084..11446)
                     /gene="rpsF"
                     /locus_tag="VF272_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7259859.1"
                     /translation="MRPYELVVVLHPDLEIDVDAPIAKIERLVGGVNGTITKRDNWGK
                     RRLAYPVKKQQYGIYVYFELELPAEAVREIETQLLIIEEVIRFLLVRKLILQRNPENQ
                     SKPRSTEELKPATAASKE"
     gene            11601..12128
                     /locus_tag="VF272_02935"
     CDS             11601..12128
                     /locus_tag="VF272_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259860.1"
                     /translation="MYVLANQITALQVMSLQTGQPIAVIERPLINAANLEVKALRCKM
                     NRRKTGVILMRDIRQFANDCVIIDSFDEIEDANEIVRLRDVIKRDFNPIDKLVINESN
                     HRLGRVEDYTINLKTFMVQKLYVHQSLVKSILFNNLVVDRTQIIDVTDRQFTVKDAGV
                     IEPSLSPQPASPPPK"
     gene            complement(12261..12881)
                     /locus_tag="VF272_02940"
     CDS             complement(12261..12881)
                     /locus_tag="VF272_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX7259861.1"
                     /translation="MKITRISQQQVRKERFSVYVDGEYRLSLSQDQLADAGLRSGDEL
                     TPAQFTQLAHLSELGKALEGAYRFLSYRSRSRQEVEQHLRRKQYDASLIDEVIAQLEA
                     VGVLNDLLFAREWIANRQLLNPRSPLELKVELRQKGISPGLIDEAVASMTWDNEVVMI
                     ESLIAQKKLRRRYPEERKLLSYLASKGYSFQAIRTVLEANNEDKTI"
     gene            complement(12900..>13342)
                     /locus_tag="VF272_02945"
     CDS             complement(12900..>13342)
                     /locus_tag="VF272_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEX7259862.1"
                     /translation="SKSNTTVIFINQLRMKIGVMFGNPETTAGGQALKYYSSVRLDIR
                     RSEQIKEGDRVMGNKVRVKVVKNKVAPPFRTAEFDIMYNQGISASGDMLELAVKFDLV
                     KKSGAWYEYKGEKMGQGREAAKAYLDEHNKVMKELEAAIRKAAA"
BASE COUNT         3280 a         3413 c         3390 g         3259 t
ORIGIN      
        1 acgttcaacg gtcgccgggt tgcttggcag gcactctaca ccgtgggcat cttcccagta
       61 tttaatccag cagagcttgg cgacattgct aaagcgtgat ccaatcggct catttccgcc
      121 aaacttaaca ttaatgataa agtgggctgg atggggcata ttgtcggttg gccaccgaat
      181 ggcgccccag gccaccgtgg agctgcaggt gatccgctgg aaatccggga tgcagttatt
      241 ctctgacgac tgaattaccg ggtttaaagg atctgcacct aaatcggcat agttattatt
      301 cgttgcatca agtacataat attcaattgg tccggcagtt gtatcgggga caacctcaat
      361 cttatacgaa ccattatcgt atttaatgtt agcgggatca tattcgcaca ctccatcccc
      421 gtctaggtcg cattccggaa ggcctttgcc tccaatcgta accgatttta cgggcggtgc
      481 aacccggccg attataggat gaaatactcg attcgaccag ccggtaaatg aattatcaac
      541 cgatgcgtag gtataattca ctactccctg taagccctcg gacctatcgg gtttttcaca
      601 ttggcttaac catcctggat tggtaaaatc tgttgccgag ccattggcgc aaaaactatc
      661 aacggttgca ccggcaggat agacgccaat atcggcggca tccgatttca ccgttccgtg
      721 ccattttatg ataagcgtgc tgtatgccgg catctccaaa aactctaagg cgatatgagc
      781 tggagccgcg gttgtcgcgg catgaacatt acagctaaat tccgtgtaaa agttttttga
      841 gtaaccatac cctatgggga tgttaaaaac cgaaccatct ttacgacata agttgtcggc
      901 acctggccct gtgtaaaaaa acgtttccga actccatccg ggaggaggcg aaccgctatt
      961 tatgaattgg acgctatcaa caaacacatc ggagagaccc tgcttcatat tgagcggtat
     1021 attgtcggag atgtttacat ttgagtaatt gccgtcacga ttattttgta ccgtgaacgt
     1081 atagtcaatg cgctggccga gtacaacggg tactccgttt ccgggggtag cgcctttgat
     1141 cattggcgga ggaattggca ccggaacata gacaaaatca tatttgtcgt cggccgtccg
     1201 atcataaagt tgcacgtcgc tccggttaca ggcaggataa aagttcggat tacccaaatt
     1261 gcccggcccg tcgcttcggc aattcatacc gggaacttga tgaagatagc cgccgtcggc
     1321 ctcgccatta ccctgggtaa taggattacg tagaacatac tgggtgccat ccggcccggt
     1381 atactcgttg ggagcggtta tacacacccg ggcgccaatc acggcccgga catcataatt
     1441 tccgctggaa ttggtgcgtt cgctggcacc agccttgata tcgccgcctt cacagttact
     1501 aaaggttaat tgcacgcctg ggagcgctct cccggtggct tcctcatcgt ctgaggtaac
     1561 gcttccactg agggtggtgg ggctcaggaa ataacagcgc atatctccaa gcctggcaat
     1621 cctcctcata tcattgcttg cgctaatact ggctgcaggc tgatcttccc ctagcgatac
     1681 atgattgcgc aggcaaaact tgctagcgga atcgctcggg accgtatcgg ttgaaacgta
     1741 atcaggcaag ctaaatttaa atctataacg aatgtgatta ttgccggcat cttggttgcg
     1801 catccagaca attgacttac cctgtaagcc gcagcctacc cagccagttg gctcgggagg
     1861 atcattatta atgccggaat acggaccgac cgcaaaatct tcgagcggac aggcattaaa
     1921 aacgtcaccg ggacttcccg gaccgggccg gcaaccggga gcagcgctat tcggatcccc
     1981 tccgcatgcc gccggcattg gcgtatcggc cgccgtacca tcattatatt gaatatcatt
     2041 ggatggcccc tgcgttgtac catagagccg tattccctga gtataatcaa acaaggcatc
     2101 acgaatgtcg aggatgaccc agttatagac gtttcggcca tttgtgccac ctgatggctg
     2161 atagctgtca tatccggttt cgtagcggaa tgtcgctcct ggtccaacct gctgcggagc
     2221 atcaatatcg gtactgccgc ccggcgtgcc gtcgattggc gtaagcttta cgttgtttgc
     2281 aagatcggca gtgtcatcat tgggacataa aaaaccatac ggaaagtttg agctgctgca
     2341 catagccgcc gaatgcgacg ccaaaatctg gttgccgccg cttaccgcgc caaccaacgc
     2401 cagcgcgaaa acggtggcca ttaagcatac tgcctttttc catcgactca ttacactcat
     2461 agatacatta atgatataag cataagcatt aatattcaat ctaaaaggaa aggccccggt
     2521 tgagtttcct cagccggggc ctgctcccta ctgcgctaca gccgcttgcg acccgtgagg
     2581 gtcatcgacc gcatctgggc gggatcgcgg tagcggagct gacactcccc tccgcggagc
     2641 caggtgagcc gaaagccatt cgaccgaacg tagtcgccgg ggcgcagcct tacctggctg
     2701 ccgtagaacc ccagaagcat ctccttgccc cacgggcgca ggcgccgatc ggggccgacg
     2761 tagggacggc agcggatcga ttccaggctg cccttatggg cttccaccca gacacacagc
     2821 accgcgccgc ggcgcggacg cgccgtcacg gtgacgtagc tggtgccagg cagcatgcgc
     2881 ttggtgagac ggaggcgacc caagtcgccg gtgtttgcct tcccgccagc gttgtactgc
     2941 acccgatagg cgttggactt gcagtccgcg ccgctgtgct gcgcgtacgc cgacatgacg
     3001 gaactgcccg acgccggggt gaccgcagtc atgcacgcag cggcaaacgc cgccgcgagt
     3061 actgcacgaa cgaacttcat gtaaatcgcc tgctttctat ttgaacctgc tagctcaaga
     3121 tcgaagggta cgctaccgat atgtgcctgt agggaatgca cattcagtcg tttgtctgtt
     3181 atacacctat tttacaaaat atgcaagcat aactattttc ttatcgcctt gtaagattct
     3241 tgaagaagct atatatttaa gcataagtaa tactaaggcg gtatatgaaa ttatctctcc
     3301 ctgtaaactt cccgacgaaa gcatcattac aatcgtggcc ggctaaaaac tatttcatcc
     3361 ttggaggagt tttacttttg ctcctgcttg cggcgggagg catttacgcg gctttccttc
     3421 cacctaccag caccataaaa gttcaaacct acgaggttgt taatggtacc ccccagcgct
     3481 taggcaacgt aacggttcag cttgacagtt ataaatcgag cgcagaatgc gccgactcta
     3541 ctaaaacaac ggaacccgca tcaactggcg ccaatcatgg aatcgttaac ttctatggct
     3601 gcccgacagg gattcgctac acaatagcaa acatgagtcg aagcggttat gttcaagctg
     3661 caaacagtcc tataaaaaca aaaacaagcc ggttttattc gcgcaagaat cgggtgacaa
     3721 ctattaatct ttacctggtt aaagccggtg acgccgcgcc gattagaata agcggcccaa
     3781 gtaaaaagaa taatatttat aacaccgctt ctagggatca tttctacaat aaggttaatc
     3841 ccgatggcgc ttggatcatg aactttaccg acacctacta catcggcatg gtatacgacg
     3901 gatggtcgat tgatgttgcc aagaaaagcg aggctggctg gtattttggt ttagtaagcc
     3961 gccctgacgg cacggtgatt ggttgcgcct ggatcgaacc ggaaatcgta cgtcccggca
     4021 ctgacactcc ttccgctaat agctgcagcg gccaagatgc tacccttaaa gacagagaca
     4081 gggttgggat ggactttaac tgtgctttta atactgatgg ttcatgcaat cggccggcaa
     4141 gcgccgtgga ggtcggacca aattgcgata caaccttata taagaactat tttacgaata
     4201 acaattatga atccggtagc tttgcggatc cggtaggaaa aatagaatcg tttcccacca
     4261 atttcgatcc tccgcaacga gctggctacc gatttactac gcatgacgag aaagctgcgg
     4321 tcgttttcag ttcaacgtat ggatggggct tcatcgatag aacctgcatc ccagggactc
     4381 cgcggggaac aaattagtgc ggggtgacgc cgcggcttga tcgttaagat catggccgcg
     4441 gcgcgttccc tcatccttga gactatcgcg cgaggcgcat ttggaagact agctgttggg
     4501 catagccctc ggctttacac ccgttcttgg tgttggcgtt gagacgccaa cagaagatgt
     4561 actcgccggg cttgagcccg acgggcaagc tgacgacgaa tcgccagttg ttgttcatca
     4621 gcttcgtcgg cttggggctg ttcagcccgc gaggccgatc ttggctcaga aacgagtccc
     4681 tgagcccgaa gctgatgacc ttcatggccg agtggttaac cacgagcacg accttcgccg
     4741 gactacttga gccctgaggt gacagcttgg cgctgatgac ctcgggctgt gaggcggcgg
     4801 cgggggcagc agctgcaagc gccgctacca gggtcaaaac cacaggtgta aagcgacatt
     4861 tcatactgat tgcctcctcg gcagggtgtc tagttggatg ggggcttgat ccgacgaagc
     4921 tcagtgctaa atcgaatcgt aataaaacat tatacagtat gtatgttatt ttgtcaatag
     4981 gcagatccgg gtatacctcc cctactgccc tttgttcttt tttttgacag agctttcata
     5041 agcaaccata ataaacgtaa ccatttatac aaggtgtgta gcggaagtgg caaataagaa
     5101 acatccagta aaatttacac gatggatcct ccccggcata ttggctggct gtcttttgct
     5161 tataatcact atgctttcgg tgatggctgc cagggcggca gcgtttgatt ataaggggaa
     5221 tattgaggtc aaggtcattg actttaatag cggtacgtat cttccgatta ccaccgctgt
     5281 ggttagcgtg aaggtaatag gcgccgccac caactgcccc tcaccgacgc aaactaccga
     5341 tgcaaacgga agagccattt tcgttggctg cccggcttac tccacaggcg tgaaaaaata
     5401 caagttagtt gatgtgagcg cccccggcta tgtggtgcgc ccaaacagca attataaaaa
     5461 agccacttcc ggattttatt tattcggcaa aggcaggaag aaaacccgca ttcgtgtcta
     5521 catgcagcgt gctcaagccg cccaggcacc gcgaccgact cggccgccat caaacgcgaa
     5581 tgacccttgg ggagcatcac ccaatgacaa cacacccgtt gcatgcaggg cggtattagc
     5641 acccggcatt gctcctctgg cctgggcaac cgacgccggt tactatgcta acgacgctaa
     5701 aggatttaac cgaatgatgg acgtgctatg aataatctta ttttaatgag tcagaaacgt
     5761 ataattttgg cggcaatcgc aattaccaca atcgtggtaa ttgcaggggg aatatacctc
     5821 gcgatatcct ataaaaatcc ccaaagccca gccgaaaccg tctattctga tccggatctg
     5881 caagacatta cgtccgttaa cggcaagcgg ctccaagaaa ttaccttgcc caacggcaaa
     5941 aaaaccaaag tgcgcgctga agtacttatg gtgcggataa ccgatccaaa caccgttaaa
     6001 actttgggcg gccgggcaac tcccttcccc ggcattccgc aggtatatga agtgaaggtt
     6061 ccggttggta aagacctgat gcaggccaag gctgaactgg agaaaatgcc gggagtagcc
     6121 tatgccgaac cagactatat aaatttcgca caagctctac cgaggattat tccgaacgat
     6181 actcatatag cgaatcaagc atcattaaat gatatatcgg ccccagaagc ctgggcgctc
     6241 aataccggca gcagtggtac cactatagcc attattgatt cgggtgcttg ggggacacac
     6301 cctgatttgg taaataaata tagtataaat gccgccgacc ctgtcgacgg ggtcgacaat
     6361 gatggaaacg gctatgttga tgataacttt ggcatggatt ttgtgcgggg aacaataagg
     6421 gacgcaaacg gtgacggcac cgttgaatgg gttaacgatg ctaatggagc cgatgacgac
     6481 aaccacggtg gtcttaatgg tgacgggcat ggaacggctg tggccggagc agctgccgcc
     6541 caatccaaca atggtgctgg cgtagccagc gttaactgga atgcccggat tttaccgatt
     6601 gctgtaggca gtgatggagg cggtgatatt acgtactttg aatcgcatgt cgcggctggc
     6661 atttactatt ccattgcccg cggagctcag gttattagcc ttagtatagg ccgtttgtgc
     6721 gccgctgaaa ccacatgccc ttccgagcta atgcggcaaa gtatgcaggc ggcccaagcc
     6781 tccaacgtgg taacggtagc ggctaccgga aataatagta cgagcaatga aatacataat
     6841 atagccagaa tcgccactat cgcggttgcc gctatagata ccggcacctt aaccaaggcg
     6901 tcttatagca atagcggtcc tgaagttgat attggagcgc ccgtacccgt tgttaccact
     6961 caaaaaggcg gcggctatgt tccaatggca ggaacatcgc tagcaacgcc tcaggtagcg
     7021 gcggcggcgg cttatgtgct cggtcttaac cctggccttt caccggctca ggtcttaaat
     7081 gtgcttcaag ctacagcaaa tgcccctcct ggaggaagaa ataatgatgt tggagccggc
     7141 aggatcaatt tgtataaact aattaggttc tgccaaacct gcatataacg cgtttataag
     7201 taaggaaaaa gccccgcaaa tgcggggctt tcccttgaag caacttctga tggccctatg
     7261 agccgaacat cccctgaggg gagcccgatg gggcgacgct gttcgtcagc ccctcgatca
     7321 cgagccgcat gcggagcgtg cgcagcttgc cggggcacac gacgggcgac actgagttga
     7381 tgctgtagag cgtgccgagc gagggtggga agtcggtgat ccaagcgctg atgcgactgg
     7441 tgttgccgcg agcggacttg gtgacgtgct ccggatcgaa gcgctgccaa gtccctcctg
     7501 ccaccttgcg ctcgaaccag atgcggacgg cgagcggacg gcagtgacgg ctgttgtacg
     7561 tgcgggtgac ctttccgtgt accatggtca ccggtgccgg aatgtcgtag ctcagcgtgg
     7621 ctgagccaac ggcggagccg gcggtcttgg cgacagaagc gttggcgctt ccgggcacga
     7681 ggaggacaac agctgcgagg actgcaagag ttgcggcgcg catggtgagc ctttcggatc
     7741 gtacggcggt ttcagaagga aaacttcaag gggcatctac tgacacggct atatattata
     7801 catgaaatgc attttttgtc aataggcagt tttaccaggt atgcctacgc ttcatttcct
     7861 tatctggcag ttttctgctc ctgtatactg cttttatgaa gatagcactt acgggactac
     7921 ccaatgtggg caagtctacc ctttttaatg cgctgaccaa aaataatgtt ttggcggcta
     7981 actatccttt tgctaccatt gagccgaatg tcggggtcgt gccggtccca gatgagcgcc
     8041 tagcggtgct tgctaggctg tatgatgcta aaaagatcgt accggccaca gtaacctttg
     8101 tcgatattgc cgggctggtt aaaggcgcct ctgaaggcca ggggcttggc aatcagtttt
     8161 tagccaacat tcgtgaatgc gacgctattt gccaagtggt acgggcgttt agcgatccta
     8221 atgttcagca tgtttcagaa aaagttgacc ccctggatga cattacaacc ataaacacgg
     8281 aactgatact ggccgatctg caaacggcag ataagcgatt gagtaagctc aagaaagatg
     8341 ccagccagaa agcgctgcta gcggaactaa ccaaggctcg ggcgattctg gataaaggag
     8401 agttgctggg aagccatcct gagcttgacc gcgaggtgct aaaagaactg caatttatga
     8461 cgctcaaacc ttttgtattt gcttttaacg tagatgagga tcaactcagc aataccgaac
     8521 gtcagaagga actgacggcg ctggtatccc ctgctccatg cgtgttcctg tgcgcaaagg
     8581 tagaggccga gctgagcacc cttcccgaag ccgatgcggc ggagctacta catgagcttg
     8641 gccaagccga gcctggatta gtagctctgg cccgcgaagg acagcgcgct ttgcagttgc
     8701 aaagttattt gaccgccggg ccccaggaag tacgggcctg gaccattccg ataggagcca
     8761 ccgccccaca agccgctggc gtcattcata ccgattttga aaaaggcttt attaaggccg
     8821 aggtagtgag ttatgaggac ctggtgggcg ccggttcgtt ggtggctgcc cgaggggccg
     8881 gaaaagcccg attagagggt aaagactatg taatgaaagc ggatgatgtg gtggagtttc
     8941 ggtttaatac gtaagtaagc caccagcgaa cctgccaatg gcgctcacga tgcattaaga
     9001 aagtgataca caaagtgtgc cgtttaacac actgcgtata tcaagaaatt aattgcatgc
     9061 gaggcgtaat gggttgctcg agtcaaatca gcgtgagcac gtagaatcgt tcgcgattgc
     9121 cctgggcgcc atgaaccgct gagtcggccg atgcggtaat ggtaaattgt tcagccatta
     9181 ccgcctccag ttcagctaaa atattcgttc gaaccttttc atccttaatt acccctttat
     9241 atcgatcggc cgttggctta tcagcctcaa actgaggctt tgccatagct ataatctgtc
     9301 cggccggagt aatgagctgc gccaccgttg gcaagatttt ggtcagcgac acaaatgaca
     9361 catctataac cgccctatcg ggaagcggat cgagctgctc ggcttgaacc gtgcggatgt
     9421 cggttttttc catgatgata acccgtacgt cttggcgtaa ctgataggca agctgagcgg
     9481 taccgacgtc aacggcgtat attttggcag ccccgtgctg cagcgcgtaa tcgctaaatc
     9541 cgccggttga agagccaacg tcaaggacta ctttgtcgtt aaaatccaat tttagggcct
     9601 cggctaccga cgccagtttg tctccgccgc gtgaaacata gcgcggacgg tcggccactt
     9661 caatttcggc atcggtttca accagtagcc caggcttcac taacttggtc gtattgctca
     9721 taacctcccc cgccataatc atagcctgag cggctgtgcg agttggggca agtcctcgtt
     9781 ctactaaaag aagatcgagg cgttgtttat ttttggcgct caagatattt gccgtcgcgg
     9841 gtatcaacct ttataacgtc accctcattc acaaacagcg gcaccataac ttccagcccg
     9901 gtttccagcg tagccggctt caatgccgcg gtggcggtat cccctttcac ccccggctcg
     9961 gtgctcgaaa cttgcagaaa aacattgttg ccaatatcga cgccaattgg cttttcgtca
    10021 aagtacataa ctatggcggt actaccctct ttcagataag aagtcgcatc aggtgctagt
    10081 gagcggttaa tttgatcctg ctcataggtt tcttggttca taaaatgcaa gctatcatcg
    10141 tcattgtaaa gaaactgcat cgtgcgctta gcaatatcgg cagccggcac cttatccgat
    10201 gagcgaaacg acttatcgaa actggctccg gttatcaagt tctttagctt ggtgcgcata
    10261 acggcgccgc cgcggcccag cgccaggtgc gaggaatcga tcacttggtg cggaacaccg
    10321 tcgagatcaa tcactacacc tttccgtaat tccgtatgtc cgtagatcat gaggagaacc
    10381 ctatcttaac tcactacaaa cggtaaaaat gccaaatgcc gggcacgctt taaggcaatt
    10441 gctacccggc gttgatgctt taaacaggtg ccagttcgct tgcgggattc tattttgccg
    10501 taactattaa catagcgctg gagcagttta acgtctttgt aatcgacatc atcaagcttt
    10561 tcagcacaaa aacgacagac ttttttactg taaaaatttg ccatggtatt ccttcgttaa
    10621 aacggtattt cgtctaggtt aacgggtgca tcatctttca aatcttcaat gacgacatcc
    10681 tttttcttgg cagctggctt agcaggggca tcggaagaag attcagcagg atgatcactg
    10741 ccagcttcac cgggacgatc aagaaagttg atatcgctcg caacaatttc agtacgctga
    10801 cgcttaatgc cgtctttttc ccagctctga gtctgcaagc ggccgaccac cataaccttg
    10861 cgccccttgg ccaagtattg gctggtaagc tcaccgagct taccccaggc aacaatttca
    10921 tgatattcaa ctgcttcttt acgttcaccg ccgccatcaa cccagctacg gttggtcgcg
    10981 accgcaaacg aagccactgg ttggccgccg ggcgtggtcc gtaattcagg gtcacgggtg
    11041 aggttgccca ttacaatcgc ttgattaaaa ctttttgcca tttctactcc ttgctcgcgg
    11101 cggttgccgg ttttaattct tcagtggagc ggggttttga ctggttctcc gggttacgct
    11161 gaagaatcag cttgcgtacc aggaggaagc gaataacttc ctcaatgatt aacaactgcg
    11221 tttcgatttc acgaaccgct tcagcgggca gctcaagttc gaaatagaca taaatgccat
    11281 actgctgttt tttaaccggg taggccagcc gacgcttgcc ccagttatca cgcttggtta
    11341 ttgtgccgtt aacaccgcca accaaccgct ctatcttggc aatcggtgca tcaacgtcaa
    11401 tttcgaggtc ggggtgtaaa accacaacca attcgtaagg tctcatagtg cccctttctg
    11461 tgggatccgg acctctccgg atgccgtccg gccgatgcgt atatgcgcta aggctgaagg
    11521 gcaaaaaggt tacttaattt gacacttaca atactacaga tcacttatta aatcaaggtt
    11581 aaataggcta cacttgtagt atgtatgttt tggcgaatca gattacagcc ctgcaggtca
    11641 tgagtttgca aaccgggcaa ccgattgcag ttatcgagcg gccgctcata aatgctgcga
    11701 atttggaggt gaaagccctg cgatgtaaga tgaatcggcg caaaaccggc gtcatcttaa
    11761 tgcgtgatat ccgccagttt gcaaatgatt gcgtcattat cgactcgttc gatgagattg
    11821 aggatgccaa tgagatcgtt cggttgcgcg acgtaatcaa gcgagatttt aacccgattg
    11881 ataagctggt tattaacgag tccaaccacc gcctgggacg ggttgaagat tacacaatca
    11941 atttaaagac gttcatggtt caaaagctct atgtccatca gtcgcttgta aaaagtattc
    12001 ttttcaataa tctggtagtt gaccggactc agattatcga tgttaccgac cggcagttta
    12061 cggtgaagga tgccggcgtt attgaaccgt cgctatcacc gcaaccagca tctcccccac
    12121 ccaagtaacc agccatttaa gcattgctgt tttaaaccgc ttgcgctaca gtggtaggta
    12181 gtaagtgagg ggtagtgtta tcattacgat tcttctgtgg gttctggctt ttggcctcgt
    12241 ctatcgcgtg ttagcagctg ctaaatagtt ttatcttcat tattggcttc taaaacggtt
    12301 cgaatggctt gaaaggagta cccttttgag gctaagtagc ttagtagttt gcgttcttcg
    12361 ggataccggc gacgcaattt tttttgagcg atcaagcttt cgatcatcac tacctcatta
    12421 tcccaagtca ttgatgccac ggcttcatca atgagaccgg ggctaatccc cttctggcgc
    12481 agctctactt tgagctctaa gggactgcga ggattaagaa gctgacggtt ggcaatccat
    12541 tcacgggcga acaaaagatc attaagcacg ccgacggctt caagctgagc gatcacctca
    12601 tcgataaggc ttgcgtcata ctgtttgcgc cgcaagtgct gttcaacctc ctgccgactg
    12661 cgcgaccggt atgaaagaaa gcgatatgct ccctcgagag ccttacccag ttccgacaag
    12721 tgcgctaact gtgtaaattg ggcgggggta agctcgtctc cgctgcgtaa tcctgcatcg
    12781 gctagctgat cttgcgacaa ggacaatcga tattcaccat caacatacac tgaaaaccgc
    12841 tcttttcgga cttgctgttg agaaatgcgc gtaatcttca ccgtagcagc agcaggcgcc
    12901 tatgcagctg ccttgcgaat agccgcttca agctctttca taaccttgtt gtgctcatca
    12961 agataggctt tggcggcttc acgtccttgg cccatttttt cgcctttata ctcataccaa
    13021 gcaccgcttt tcttaacgag atcaaattta accgccaact ccaacatatc gccggatgcc
    13081 gagatacctt ggttgtacat aatgtcgaac tcagccgttc gaaaaggcgg tgcaaccttg
    13141 ttcttaacca cctttacccg aactttatta cccattaccc ggtcgccttc cttaatttgc
    13201 tcggaacggc ggatatccag acgaaccgat gagtagtact taagcgcttg gccgcccgcg
    13261 gtcgtttccg gattaccaaa catgactcca attttcatgc gaagctgatt aataaagatt
    13321 acggtggtat tagacttgct ga
//
LOCUS       DASVGB010000094         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_303482, whole genome shotgun sequence.
ACCESSION   DASVGB010000094 DASVGB010000000
VERSION     DASVGB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303482"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..963
                     /locus_tag="VF272_02950"
     CDS             124..963
                     /locus_tag="VF272_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7259863.1"
                     /translation="MNPRKTLLAALDDLGIRRFRLMGPWDLIQPTPTAYDFADLDWQI
                     AQIAKRDGEVSVTLGLRQPHHPECHQPAWARKRSSAEWQEALFAFITTVVNRYKDQAV
                     VVSWQLENEALNKDFGQCADFNQERLEAELQLVKRLDPSRPVIMSLKNHWSFLAPGPI
                     PDVYATSIYLCVHRKGKLRDTILPPLYQRVRALYIRLQFNRSFFIHELQAEPWCPRGT
                     QHTPLAEQDLSMNPKRFRHAVAYALRTGLKPIDLWGLEWWYYRKTRFDDPTMWDEART
                     VFK"
     gene            1150..2001
                     /locus_tag="VF272_02955"
     CDS             1150..2001
                     /locus_tag="VF272_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259864.1"
                     /translation="MRIKTLRQGAIGIAAAALGLAGIASAQSTISTTGPGSVNIIASS
                     STNTATLSNNNHVGVSNLNTQIAVSGPANVSGNTIGGSALSGNASNTNGTITWVSILN
                     GGLLGTIIIPTTGGGGGSIVLTGPGSVNIIGSTSNTTLTAINTNIVGVNNNNNQVAVS
                     GSANVSGNTIGGSAASGAAGNTNTAATTVAISNAANIPVLTPGTSGGGGSISLTGPGS
                     LNTISSTSNNTVTEVNTNVVGVNNTNNQTAVSGSANVSGNTIGGGATSGSATNSNTTT
                     IGVTISN"
     gene            2161..2622
                     /locus_tag="VF272_02960"
     CDS             2161..2622
                     /locus_tag="VF272_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259865.1"
                     /translation="MPDSDDRMDPDHRRYIRKVTLPSGKTIEVIDFENASDGMQLTPQ
                     VQRAEIIKEEAEATDLRICPECHLDLVYPVSGVEIGSTSWKVELLCPNCWWTATDVFD
                     GVTIERFDEGLQESTEDLIDALRRLARANLEDDFNAFIDAINAGAILPEDF"
     gene            2891..3286
                     /locus_tag="VF272_02965"
     CDS             2891..3286
                     /locus_tag="VF272_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7259866.1"
                     /translation="MLTGRPNDAQLEMLIAALLKEMSAEAGTSSTEAILRNGLAKGFK
                     RLSRKLPPTFLPQDPDTRKKIALTPHQYRIVWEVPTGKSPKRIALALHLPRDSVRVQL
                     SKVYKKTGVKGCVELRQKMERNQFFFYEE"
     gene            3605..>4264
                     /locus_tag="VF272_02970"
     CDS             3605..>4264
                     /locus_tag="VF272_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7259867.1"
                     /translation="MLVTLFQAVLGGVIAATILLLYRIAPQSLENGSLTIVIAGLGLT
                     AIVYYASIHQLLKKNHPKLSGAIISLISAANVGMVIAATGGIDSPYYALWILALLVAG
                     MFGGIAILIVVAATLSLYAYQLYPHLNEPAYLVAHVGQLGITLSAAILAEWVHRALGR
                     SSSQHEKVEALSGKLSQEALKSNVLMNSVGEGVLVISDDHQIQLFNPAAVRMTGWDEK
                     SA"
BASE COUNT         1115 a         1082 c         1056 g         1011 t
ORIGIN      
        1 agatttattt tagttttcaa acttataaag tggctgttgc ggcggcggaa acgaacctag
       61 ccaatagcta gcccttattt atttggcgca agtttttcat cccgctatgc cgagcatttg
      121 ggggtgaatc cgcgaaaaac tttacttgcg gcgcttgatg atttaggtat acgccggttc
      181 cgcctgatgg ggccatggga ccttattcaa ccgaccccaa ctgcgtatga ttttgctgat
      241 ctggattggc aaatagcaca aattgccaag cgagacggcg aggttagcgt aacccttggg
      301 cttcgccagc cgcatcaccc ggaatgccat caaccggcct gggcgcgcaa gcgttcaagc
      361 gcagaatggc aagaagccct atttgctttt attactaccg ttgtaaaccg atataaggac
      421 caggccgtag tagtgagttg gcagttggag aatgaagcgc tcaacaagga ctttgggcaa
      481 tgtgcggatt ttaatcaaga acgtttggaa gcggagctac agctcgtgaa acggctagat
      541 ccaagccgtc cggtgattat gagcctgaaa aaccactgga gttttttggc acccggacct
      601 attcccgatg tgtatgccac ctccatctat ttatgcgttc atcgtaaagg aaagctgcga
      661 gatacgatac tgccaccgct ttatcagcga gtacgcgcac tatatatacg attacaattc
      721 aaccgctcct tctttattca tgaacttcag gctgaaccat ggtgccctcg agggacacag
      781 catactcctt tagctgagca ggatctgtca atgaacccga agcgctttag gcatgcggtg
      841 gcctatgcat tgcgaaccgg ccttaagccg attgatttgt gggggcttga gtggtggtat
      901 taccgaaaaa cccgctttga cgatccgacc atgtgggatg aagcacgcac ggtgtttaaa
      961 tgattttagg atttcaacgt aagcgccgta gcgcatcgtt attaatacaa tctgcatccg
     1021 ctcggattct taaaaacatg taggaatgca cagaacggtg accaataaat tcatttatag
     1081 ttataagcaa gttggtggag tgaccagcta ataagaaatt aagtaagtaa gaacgaaaag
     1141 ggggggtgca tgagaataaa aacattacgt caaggagcga tcggcattgc ggctgcagcg
     1201 ctaggattgg ccggcattgc gtcggcccag tccaccatta gcacaaccgg accaggttcg
     1261 gttaacatca tagcttcgtc gtcgaccaat accgcgacgt tgagcaacaa caaccatgtt
     1321 ggggtttcca accttaatac ccagattgcg gttagtgggc cagcaaatgt aagcggcaac
     1381 actattggcg gatcagccct ttcgggtaac gcttccaata caaatggcac aatcacgtgg
     1441 gttagcattc tcaatggtgg cctattgggc acgataatta taccaacaac cggtggcggg
     1501 ggaggcagta ttgtcctaac cggcccaggt tcggttaaca ttatcggtag tacatcgaac
     1561 accacgttaa ctgcgataaa caccaacatt gttggtgtaa acaacaacaa taaccaagta
     1621 gcagtaagtg gtagtgctaa tgtaagtggc aacactattg gtgggagcgc ggccagtggt
     1681 gcagccggta ataccaatac cgcagcaacc acagttgcaa tttctaacgc tgcaaacatt
     1741 ccggttctta cacccggaac gagcggaggt ggaggatcga ttagcctaac cggccccggt
     1801 tcacttaaca ccattagcag cacgtcgaac aacacggtga ccgaggttaa caccaatgtt
     1861 gttggcgtaa acaacaccaa caatcaaacg gctgtaagtg ggagcgccaa tgtcagcgga
     1921 aacaccatcg gcggcggtgc aaccagtgga tctgcaacca actccaatac gacaactatc
     1981 ggagttacga taagcaacta acagatatca atacaaaata gtttaaagaa agggcgaaag
     2041 ccctttcttt ttatttacct attcgacttt acgacattat ttgcttttat tcaaaaatat
     2101 ggtatacttt tgcaaaatac acacacctgg gcttagcccg ccaaacaaaa aggaccactc
     2161 atgcctgact ccgacgaccg gatggacccc gaccacaggc ggtacatccg caaggtcacg
     2221 cttccttccg gcaagaccat cgaggtcatc gacttcgaga atgcatctga cgggatgcag
     2281 ctcacgcccc aggttcaacg cgctgagatc atcaaggaag aagccgaggc caccgacctt
     2341 cggatttgcc cggaatgcca tttggatctg gtgtatccgg tctccggtgt cgaaattggc
     2401 tcgacttcct ggaaggtcga gctgctctgc ccgaactgct ggtggacagc aacggacgtg
     2461 ttcgatggag tcaccatcga gcgcttcgac gagggattgc aggaaagcac cgaagacctc
     2521 atcgatgcct tgcgcaggct ggctcgcgcc aacctggagg acgacttcaa cgccttcatc
     2581 gacgcgatca acgccggtgc aatcctcccc gaggacttct gacctcacca agccccgctc
     2641 gtgcgagcat cacgcaataa tgtgaatgcc tcgcctgggg cggggctttc accttttatg
     2701 cccgttaaat ctctgtttat ttttagctcg ggcccaataa cattgctttt tgcggtggga
     2761 tagtattatt agcagctact cacggaaaaa acacgccaaa aatagtgtgc ggccaacatc
     2821 tcagatttgc acttattgcc gaaaaacgat ataagggtat aaccttgatc ttcgacatca
     2881 ggagagtcaa atgctaaccg gacgccccaa cgatgcccag cttgaaatgc tgattgcagc
     2941 cctcctcaag gagatgagcg ccgaggcagg aacgtcatca acggaagcaa ttctgcgtaa
     3001 cggtcttgca aaaggattca agcggttgtc gcggaagcta ccaccaacgt tcttgcccca
     3061 agaccctgac accagaaaga agatagccct aactcctcac cagtaccgga ttgtctggga
     3121 ggtgcccacc ggcaagagcc ccaagcgtat cgccctggcg cttcatttgc cccgggattc
     3181 ggttcgcgtg cagctttcga aggtatacaa aaaaaccgga gtcaagggtt gcgttgagct
     3241 gcgccagaag atggagcgca accagttctt cttttacgag gagtgagccc gcgccgcccc
     3301 caaccctgcc cgatatagtg gaagccccct tgatgagcgg tttcggccat tcatcgtgcg
     3361 gggttttcac ttttaatgaa aatagcaaaa ggagctctca cccttgggag ctccttttgc
     3421 tatttttgtg tccgaggctc ctgaacacaa caagcactca aaacatgctt aaacctctga
     3481 cacctgcacc ataccataac tcgcttatgc ttgtaaacta ctagtttttg tccttgaatt
     3541 atgagatttc ttgatgcata ctaaaagaaa cataagaggt ttcgtgcagg tttcaagtcg
     3601 ctacatgtta gtcacactgt ttcaggctgt cttgggcggc gtaatagcag ctactatttt
     3661 gctgctctat agaatagccc cccagtcgct ggaaaacggc agcctcacca tcgttattgc
     3721 cggcttaggt ttaacggcaa tagtttatta cgccagcatt caccaactgc ttaaaaagaa
     3781 tcatcctaaa ttatccgggg cgattatcag cctgatttcg gccgccaacg tcggcatggt
     3841 aattgccgcg accggcggta ttgattcgcc atattacgcg ctctggattc tggccttgtt
     3901 ggtagctggc atgtttgggg gtatcgcgat ccttatagtg gtggctgcaa ccctaagcct
     3961 gtatgcttac cagctgtatc ctcatcttaa tgaacctgcc tacctggtgg cacatgtcgg
     4021 ccagctcggc attaccttgt ccgccgcgat tctagccgaa tgggtccacc gcgcgctggg
     4081 acgttcaagc agccaacatg aaaaggtaga agccttgtcc ggtaagctaa gtcaggaagc
     4141 cctgaaatcc aatgtattaa tgaatagcgt gggcgaagga gtgctggtta tcagcgatga
     4201 ccatcaaatt cagcttttta atccggctgc ggtacgtatg accggatggg atgaaaagtc
     4261 cgcc
//
LOCUS       DASVGB010000095         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_189462, whole genome shotgun sequence.
ACCESSION   DASVGB010000095 DASVGB010000000
VERSION     DASVGB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189462"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="VF272_02975"
     CDS             complement(<1..2126)
                     /locus_tag="VF272_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259868.1"
                     /translation="MMLGGLQIGGFTPQAEAAVGVNQQINFQARLLNAQGATVPDGTY
                     NIQFKIYQDGTGCVVSGTSPCGGTLLWTESWLNNTAQGVTVKNGYFSVYLGAIASLAA
                     VDFNQDTLWLSLNIGSTAPTCTPFTSCGGDGEMLPFKRFASNPYALNSGKLGGLTSSQ
                     FVQIAPGSVQADSSTASSIFINKDNAAGNILQLQKAASDVFVVSNSGTTAINTNSVTA
                     LTVQNGSNNAFGVDTTNIKVAVGLATSAASSRLSIAGQGASNGLTLGDGATGSTNLYV
                     SADDTLKTDDSLVVVGSISTLGSSSVTSGGAFIGPTSGNTINGLIVNTGALSGITSIS
                     LSGAISGGTTYSGSGDITSTAGSVVAATHTGSTAVTVSSGGSSALTLTSASGTAILGG
                     STNTIQRTNSTFALDVLNAGSVSTLNVTNSDVSQVANLNVDGGLNIGSGQTYKINNAD
                     INTAGTLTNVAYENQSNSFTSTNSFAGSGDGVNVIGVLFKNGSDSTTAFKIQNALANA
                     TILTADTSNVRVGVGTAAPQATLHVVGGGAPYTTYNGIDGTTATLGGSFLEQTVTDST
                     FTNPYRGSVNKTTVNFASDPGAKSVLGGYNEVGIPSGNATAMASLTLRANNNVVTHFG
                     TGTLGTAGGLVTAIKNMSTGTITDAIALQGQIQNQSTGTMTRAYGLQVSNISNAGTIG
                     DTYGVYIGTVTAGTQTNTPYAFYASDA"
BASE COUNT          556 a          529 c          580 g          577 t
ORIGIN      
        1 gcatcggagg catagaaagc atatggggta tttgtttggg tgcctgctgt gacggttcca
       61 atatagacac cgtaggtatc gccaatggtg cccgcattgc ttatgtttga aacttgcaac
      121 ccgtatgctc ttgtcatagt acctgtgctt tgattttgaa tctgaccctg cagggcgatg
      181 gcatcagtaa tagtgccggt cgacatattt ttaatggcag taacgagccc gccggcagtg
      241 ccgagtgtgc cggtgccaaa atgtgttact acgttgttgt ttgcacgcag cgttaagctg
      301 gccattgccg tggcgttgcc gctggggatt cccacttcgt tatagcctcc tagcactgat
      361 ttcgcgccgg gatctgaggc aaagttaacg gtggttttat taactgaacc ccgataggga
      421 ttggtgaagg tactatcggt aacggtttgc tccaagaagg agcccccaag cgtggcagtg
      481 gtgccatcaa tgccgttata ggtggtgtaa ggcgcgcctc caccaacaac atggagcgtg
      541 gcttgaggag cagcggtacc aacaccaacc cgcacatttg aagtatcagc cgttaagata
      601 gttgcgttcg ctaaagcgtt ttggattttg aatgccgtag tggaatctga cccattcttg
      661 aaaagcacac caattacatt cactccgtcg cctgaaccag cgaagctgtt tgtgctggtg
      721 aaactattac tttgattctc gtaggcaacg ttggtcaatg tgccggctgt gttaatgtcg
      781 gcattattta tcttgtatgt ctgcccgctg ccaatgttta agccaccgtc aacattcaag
      841 ttagccacct gacttacatc agagttggta acgttcaagg tggagactga accggcgtta
      901 aggacgtcaa gggcaaaagt tgaattggtg cgctgaatgg tattggttga tccacctaaa
      961 atggccgtgc ctgaagcaga ggtgagagta agtgcgctgg aaccgccaga cgaaaccgta
     1021 accgctgtag atccggtatg ggtcgccgca acaactgaac cggcagttga ggtaatgtct
     1081 cctgaaccgg aataggtggt acccccggag attgcgccgg aaaggctaat ggaagttatt
     1141 ccgctcaatg cacccgtgtt tacgataagt ccattgatgg tgttgccgct tgttgggccg
     1201 ataaaggctc cgccagaagt aacactgctt gaacctaacg tggaaatact tccaacaaca
     1261 actaaactgt catcggtctt gagcgtatca tccgccgaga catacaaatt tgtcgacccg
     1321 gtggcgccat cacctaaggt aaggccattc gacgcacctt ggccagcgat gctcagtctt
     1381 gaagaagctg cagaagtcgc caagcctacc gcaactttta tgtttgtcgt atccacgccg
     1441 aaagcgttgt tacttccgtt ctggactgtc aatgccgtga ctgaattagt attaatagct
     1501 gtggtccctg agttgctaac tacaaacacg tcgctagcag ccttttgtag ctgcaaaata
     1561 ttgcctgcgg cattatcttt attaatgaaa attgaggagg cggttgaact atcagcttga
     1621 acgctgccag gtgctatttg tacaaactga gagctggtga ggccgccgag cttaccagaa
     1681 tttaaggcgt aaggatttga agcaaaccgc ttgaatggca gcatttcacc gtcgccgcca
     1741 cagctagtaa agggggtaca ggtcggggcg gtgctaccaa tattgagcga aagccataga
     1801 gtgtcttgat taaaatcaac ggcagcaagc gaagcaatgg caccaagata tacggaaaaa
     1861 tatccgtttt taaccgttac gccttgagcg gtgttattca gccagctttc tgtccacagt
     1921 agcgtaccgc cacaaggaga ggtaccgctt accacgcaac cggtaccatc ctgatatatc
     1981 ttgaactgga tattgtaggt accgtcgggt acggtcgcgc cttgggcatt taagagccga
     2041 gcctgaaaat taatttgctg attcacgcca accgccgcct cggcttgagg cgtaaaaccg
     2101 ccaatctgca aaccgccaag catcatgagt aacccgctaa tagttagacg ggctaaaaat
     2161 tttagcttta ttaaggcttt tttagcgatt ttttgactcc atatttcaca gctggcttag
     2221 ttggtatttg ggcacactct gc
//
LOCUS       DASVGB010000096         6610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_446113, whole genome shotgun sequence.
ACCESSION   DASVGB010000096 DASVGB010000000
VERSION     DASVGB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446113"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /gene="pgl"
                     /locus_tag="VF272_02980"
     CDS             complement(<1..610)
                     /gene="pgl"
                     /locus_tag="VF272_02980"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX7259869.1"
                     /translation="MVLQVYPDQAALSEQAAKQIIALANQAVADHGSFYIALAGGSTP
                     QRLYSILASVELRSQTDWSKWQFFWSDERLVPLDDEYSNFHLADSALLSKLDLGASQV
                     HPVSIEGRTSEEIAATYEQEIRGIVQTGADTVPRFDLILLGLGSDGHTASLFPGQPSL
                     DKSQHLVVASPAGTLPPPVDRITFSLRTINAAHHVMILVSGSA"
     gene            777..1985
                     /locus_tag="VF272_02985"
     CDS             777..1985
                     /locus_tag="VF272_02985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7259870.1"
                     /translation="MLGWELPPYNSGGLGVACYYLCQAMAERNIEIDFIVPYLENHGV
                     DFMKVVSAMPVSSEITRRAGGIYDSRKWTEEMPIDADVMPGNLHQQRAHLTAFTKHYV
                     TRNKVDVIHAHEWLTFEAGVAAKKLTGKPLIAHVHATEFDRSGELYGNPLVHEIEETA
                     LSVADRIIAVSQLTKDLIAKHYGIPAEKIEVIHNGLTPAQLHATGDAASYQYLRAMKH
                     LGYKVVVNVGRLTAQKGLRYLLEAAALVVEKNPKTLFLLVGSGEIRDELITRSAELGI
                     AENIIFTGFLRGTAWRDAFAIGDLFVMPSVSEPFGLTALESLALGTPVLLSRTCGVAE
                     VARNTLKVNFWDTRAMADNILMVLDEPALHHELLVNGQHDIAGMAWLQAAERCAEIYE
                     TSARMRGVMV"
     gene            1982..3187
                     /locus_tag="VF272_02990"
     CDS             1982..3187
                     /locus_tag="VF272_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEX7259871.1"
                     /translation="MRAICLYMHVHQPFRVRPYTIFDASEDHDYFSNPESQADNHQIM
                     RKVAEKSYLPTNAALMELIKRHPQFKLSLSLSGTVLEQMQLWAPEALESFKELVATGR
                     VEIVAETYHHSLAFFYDREEFERQVKLHAKTILHHFGVKPRVFRNTELSYNNELAHWA
                     DKAGYKAIITEGWEPVLGWRSPNYVYRPAFTKQIRLLMKNYQLSDDVAFRFSNRAWSG
                     WPLTAEKYVGWLNDLDADQTNVNLFMDYETFGEHQWAETGIYDFLKHMTDLFLSSKGN
                     TFMTLSEAAKKIKPSDYIDVPQTITWADTERDLSAWVGNSMQRQAINSMYQLSDEVLA
                     TRDAGLIEDWRRLQASDHFYYMCTKWFNDGDIHAYFSPYSSPYDAFIYFMNALRDVKY
                     RVAASQGKL"
     gene            3187..5118
                     /locus_tag="VF272_02995"
     CDS             3187..5118
                     /locus_tag="VF272_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7259872.1"
                     /translation="MSRHIILSNGEMLVGLDAYGLVHDVYYPYIGQENHATARYIHHR
                     IGVWVDDAFSWLDDDSWNITTTYEEATLIGRVIAYNPDLKVRIESCDFIDADDCVLAR
                     RYKVVNDANRPREIRLFLHQAFRISNSNMGGTAQYHPKDHLLVHYKGRRAFAIYAQTA
                     SGTAFDQYSVGLFGIEGKEGTYRDAEDGVLSCNPVEHGMVDSVMRLALKLQAGGDEEV
                     QYWMAAARHPEAAITLHNEFKAEGFTARYAATKRSWKDWLHTSKPKMNAIPVKYRARA
                     VQSLLLMKSHIDQRGGVLASADSEMLNYGRDYYGYCWPRDSVNVLWPLIKLGYTEEAK
                     NFFEFCGRVRHSNGYLMHKYQMDGALGSSWHPYIHGDQPELPIQEDETAAVVFLVDQY
                     HQYADEGDYVRELYDVLVRPAANFMTDYIDSETKLPHASFDLWEMVFGTHTYTVAITY
                     AALEAASRMAKQYGSIKESKRWQRAADEIKAAASIHLWNDKRHYFYRSLRVGKDGISF
                     DETIDIASLYGAVIFGLFDATDVRVTQALETAEATLRVDTPTGWGVVRFENDDYYRVD
                     PETQGNPWIVTSLWLAKLHNILDDTATPQELVAWSASVMQRSGVLAEQIDPGTNACVS
                     VAPLTWSQAEFVDTLLELS"
     gene            complement(5111..6322)
                     /locus_tag="VF272_03000"
     CDS             complement(5111..6322)
                     /locus_tag="VF272_03000"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7259873.1"
                     /translation="MNTAEQPATHNVLIVGGGFGGVRAARMLSKQPGVSVTLLSDSLE
                     FTYYPQLYHAATGGSRSESSLPLKELLEGTSVRVLKDTSVSVDHAAKTLTTASKTVYT
                     YDQLILSLGSVTNYFNIKGLPEFCYEVKSTVSALAFKRHLHNQLIEDQATDVNYVIVG
                     GGPTGVELAGALGSYLKRITRLHKITKVNYNIEVIEAMPRILPRLPEPVAARMHQRLE
                     KLGVKVMTGAKVEAAGRDSLTVNGSPIPTETVVWTAGIANNPFFKANADQFILAKNGR
                     VEVNAQLQAQPDLYVIGDNAAVDHGGLAQNAIAQANFVAEDIMRGLHGSRRKKFRSTE
                     GISVIPVGPYWAAASWRNLALYGRPGWLLRRAADLIAYHDIESFPDAVRVWMHDFKQE
                     DNCRVCAKAAN"
     gene            6368..>6610
                     /locus_tag="VF272_03005"
     CDS             6368..>6610
                     /locus_tag="VF272_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259874.1"
                     /translation="MKNQTIRWLALGLAIALALPMIWQWYSLSAVPASTSSEYLIAIG
                     RLAGMVMTFGVLMQLILMSRLPGLEKAVGLERLVTIH"
BASE COUNT         1618 a         1571 c         1790 g         1631 t
ORIGIN      
        1 tagcacttcc gcttactaag atcatgacat gatgggcggc atttatggtt ctcaaagaaa
       61 aggtaatacg atcgacggga ggaggcagcg tacccgctgg tgaggcgact actaggtgct
      121 gcgatttatc aagcgatggc tgaccgggaa agagcgaagc ggtatggccg tcggatccga
      181 ggccaagaag aataagatcg aagcgaggaa cagtgtcagc accggtttgg acaataccgc
      241 gaatttcttg ctcataggta gcggcaatct cctccgatgt tctgccttca atcgatacag
      301 gatgtacttg gctggctcca agatcaagct tggataataa tgctgaatca gccagatgga
      361 agttgctgta ttcatcgtcc agcggcacca agcgctcatc gctccagaaa aactgccatt
      421 ttgaccaatc ggtttgggat cttaactcta ctgaggcgag tatggagtat agcctttgag
      481 gagtagatcc gccggcaagc gcaatataga agctgccatg atcggctacg gcctgatttg
      541 ctaaagcaat gatttgtttg gcagcttgtt ccgagagagc cgcttggtca ggatatactt
      601 gcaataccat agtgtaatcg tatcacgaag cctatattcc cagtgggcgt cgctaggaat
      661 gaaaaatctc aatctataga cgttgtaatt aacacattaa acagagtttt ttgttgtcgt
      721 aagtcgtatt ccccattata cttatgctta taagtaaggt tgagatgaag tcgttaatgc
      781 tggggtggga gctgcctcca tataatagcg gtggtctggg agtagcttgt tattaccttt
      841 gccaagcaat ggccgagcgt aatatcgaga tcgattttat cgtcccctat cttgaaaacc
      901 atggcgttga cttcatgaag gtagtatcgg ctatgccagt ctcctcggag attacgcgcc
      961 gggcgggcgg catttatgat agtcgaaaat ggactgagga aatgccgatt gatgccgatg
     1021 taatgccagg aaatttgcat cagcagaggg ctcacttaac ggctttcacc aagcactacg
     1081 ttacgcgcaa caaagttgat gtgatacatg ctcatgagtg gctcaccttt gaggccggtg
     1141 tggcagccaa aaagctgact ggcaaaccat tgattgctca cgtacatgct actgagttcg
     1201 accgttccgg cgagctgtat ggcaacccgc tagtgcacga aattgaagaa accgctctga
     1261 gcgtagccga tcggattatt gccgttagcc agctcacgaa agatttgatt gctaaacatt
     1321 atgggattcc ggctgaaaag attgaagtga tccataacgg tctgacaccg gcccagctgc
     1381 atgcaaccgg tgacgcggcc agttatcagt acctccgagc aatgaagcac ttgggatata
     1441 aggtggtggt aaatgtcggg cggcttacgg cccaaaaggg tttgcgttat ctcctcgaag
     1501 ctgcggcctt ggtggttgaa aaaaatccca aaacgctttt cctgctcgta gggagcgggg
     1561 aaatccgcga cgaattgatt acgcggtcag ccgaactggg cattgcggaa aatattattt
     1621 ttaccgggtt tcttcgcgga accgcctggc gcgatgcctt tgcaatcggc gatttatttg
     1681 tcatgccctc ggtttcagag ccgttcgggt tgaccgcctt agagtcactg gcgttgggta
     1741 ccccggtact actttctcgc acctgcggcg tagctgaagt agcgcgcaat acgcttaaag
     1801 ttaatttttg ggatacccgg gctatggccg acaatatttt gatggtatta gacgagccgg
     1861 cgctgcatca tgaactgctt gtaaacggcc agcacgatat cgctggaatg gcgtggttgc
     1921 aggcagctga gcgttgcgcc gaaatatatg aaacctcggc tcggatgcga ggggtaatgg
     1981 tatgagggcg atttgcttgt acatgcacgt ccatcagccc ttccgagttc gaccctatac
     2041 tatctttgat gcatcggaag accacgacta ttttagcaac ccagagtcgc aggccgacaa
     2101 tcaccaaata atgcgtaaag tggccgagaa gtcatatttg cctaccaatg cggctcttat
     2161 ggaattgata aagcgtcacc ctcaatttaa gttgagcttg tcgctttccg gtacggtgct
     2221 tgagcaaatg cagctctggg ctcccgaagc gttagaatcg ttcaaagaat tggtcgctac
     2281 cggtagagtt gagattgtag ccgaaaccta ccaccatagt ttggcattct tttatgaccg
     2341 cgaagagttt gagcgccaag taaagctgca cgcaaaaact attttgcatc atttcggggt
     2401 gaagccccgg gtgttccgca acaccgaatt aagctacaac aatgagctgg cacattgggc
     2461 ggataaggct ggatataaag ccattatcac ggaaggctgg gagccggttt tgggatggcg
     2521 cagtcctaac tatgtctacc gcccggcatt tactaagcaa atccgtctgc tgatgaagaa
     2581 ctatcagctg agcgatgatg tggccttccg attttcgaac cgagcttggt cggggtggcc
     2641 gctgacagct gaaaaatatg tggggtggct gaatgatctc gatgccgacc agaccaacgt
     2701 caacttattt atggactatg aaacctttgg cgaacaccag tgggcggaaa ccggcattta
     2761 cgatttcctg aagcatatga ccgatctatt tttgtcatct aaaggcaata cttttatgac
     2821 gcttagcgag gccgctaaaa agattaaacc ctccgactac atcgatgtgc cgcaaactat
     2881 tacctgggcg gataccgaac gcgatcttag cgcatgggta ggcaactcta tgcagcggca
     2941 ggcgattaac tctatgtatc agttatccga tgaggtctta gctacccggg atgccggctt
     3001 aatcgaagac tggcgccggt tgcaagcttc ggaccatttt tattacatgt gcaccaagtg
     3061 gtttaatgac ggggacatcc atgcctattt tagcccgtat tcatctcctt acgatgcctt
     3121 catttacttt atgaatgctc tgcgtgacgt aaaataccgg gtagcggcta gccaaggcaa
     3181 actgtaatgt cccgccacat catactcagc aatggtgaga tgctggttgg actcgatgcg
     3241 tacggacttg tgcacgacgt ctattatccc tatattggcc aggaaaatca tgccaccgct
     3301 cgttacatac atcaccggat cggggtatgg gtcgacgatg cctttagctg gctggatgat
     3361 gattcctgga atattacgac cacctacgaa gaagcgacgc tgattggcag ggttatcgcc
     3421 tacaatcctg acctcaaggt acgcattgaa tcgtgcgact tcattgatgc cgatgactgt
     3481 gttttagctc gccgctataa agtggtcaat gatgccaatc ggccgcggga aatccggctg
     3541 tttttacacc aagcttttcg tattagtaac tcaaacatgg gcggtacggc gcagtatcac
     3601 cccaaggacc acttgcttgt gcactataaa gggcgtcgag catttgcaat ttatgcccag
     3661 acagcgagcg gcacggcttt cgatcagtat agcgtcgggc tatttggcat tgagggtaaa
     3721 gaggggacat accgcgatgc agaggatggt gttcttagct gcaatccggt ggagcatggc
     3781 atggtcgact cagttatgcg gttggcactc aagcttcaag ccggcggcga tgaagaagtg
     3841 caatactgga tggcggcggc ccgtcatccc gaggctgcaa ttaccctgca taatgaattt
     3901 aaggccgaag gttttactgc tcgttacgca gcaaccaaac ggtcgtggaa ggattggctg
     3961 cacaccagca agcctaaaat gaatgccatt ccggtgaaat atcgggcccg ggcggtgcaa
     4021 agtttgttgc tcatgaaatc gcacattgat cagcggggag gcgtgcttgc ttcggccgac
     4081 tcggaaatgt taaattacgg ccgcgactac tacggttact gctggcctcg cgactcggtg
     4141 aatgttctgt ggccgctgat taagttgggg tataccgaag aggccaagaa tttttttgaa
     4201 ttttgcggcc gggtacggca tagtaacggc tatttaatgc ataagtacca gatggacggc
     4261 gctttgggca gtagttggca tccgtacatt cacggcgacc agcctgaact gccaattcag
     4321 gaagacgaaa cggctgccgt tgttttcctg gttgaccagt atcatcagta tgccgacgaa
     4381 ggagactacg tccgtgaact gtatgatgtg ctggtacgac cggcggccaa ctttatgacc
     4441 gactacattg actccgaaac caaactcccc cacgccagtt ttgatttgtg ggaaatggtg
     4501 ttcggaacgc acacctatac ggtggcaata acgtatgctg ccctcgaggc ggccagccgt
     4561 atggctaagc agtatggcag tattaaggag tccaaacgct ggcagcgggc cgccgacgaa
     4621 attaaagctg cggccagcat ccacctatgg aatgataagc gtcattactt ttaccgaagc
     4681 ttgcgggtgg gcaaggatgg catcagcttt gatgaaacga tcgatattgc cagcttgtat
     4741 ggagcggtga tatttggttt gtttgacgca actgatgtgc gcgtaacgca agccctcgaa
     4801 accgcagagg ctacgctacg ggttgatacg ccaaccggat ggggagtagt tcgatttgaa
     4861 aatgatgatt attaccgggt tgatccggaa acccaaggta acccatggat tgtgacgtca
     4921 ttgtggttgg cgaaactgca taacatattg gacgacaccg cgacgcccca agaattagtg
     4981 gcgtggtctg catcggtaat gcaacgctca ggggtgctag ccgagcaaat tgacccaggc
     5041 accaatgcct gtgtttcggt ggccccactc acttggagcc aagccgagtt tgttgatacc
     5101 ctgctggaac ttagttagca gctttagcgc aaactcggca attatcttcc tgcttgaaat
     5161 cgtgcatcca gactcgtacg gcatccggaa agctttcaat atcgtgatag gcgataaggt
     5221 cggcagctcg ccgcaacaac caccctgggc ggccatagag cgcgaggttg cgccatgagg
     5281 cagcggccca gtagggtccc actggaataa cgctgattcc ttcggtagag cgaaactttt
     5341 tgcgccggga tccgtgaagt ccgcgcataa tatcttcggc tacaaaatta gcttgggcaa
     5401 tcgcgttctg ggcaagcccg ccatgatcaa ccgcggcatt gtcgcctatt acatacagat
     5461 ccggctgagc ttgaagttgg gcgttaactt ccacccgccc atttttagca agaatgaatt
     5521 gatcggcatt ggctttaaaa aacgggttat tggcaatgcc ggcggtccag acaacggttt
     5581 cggtgggtat cggactgcca ttgacggtga gcgaatcacg gccggctgcc tccactttag
     5641 caccggtcat aaccttaacc ccgagcttct caagccgctg gtgcatccgg gcggcaaccg
     5701 gctccggcag ccgaggaagt atgcgcggca tcgcttcaat tacttcaatg ttgtaattta
     5761 cctttgtgat cttatgcagc cgcgtaatgc gcttaagata actgccgagg gcaccagcca
     5821 gctcaacacc ggttgggccg ccgccgacaa taacataatt aacatcggta gcttggtcct
     5881 cgataagctg gttgtgaagg tggcgtttga atgccagcgc gctgaccgtt gactttactt
     5941 cgtagcagaa ttccggcaac cctttgatat tgaaataatt ggtaacgctt ccgagcgaca
     6001 aaatcagttg atcataggta tataccgtct tgctagcggt tgtgagggtc tttgcggcat
     6061 ggtcaacgct taccgaggta tctttgagca cccgcaccga agtgccttca agtagctcct
     6121 ttaagggcaa tgaagattcg gatcgggatc cgccggtggc ggcatggtag agctgcggat
     6181 agtaggtaaa ctcaagacta tcagaaagaa gtgttacgct cacgcccggc tgcttgctga
     6241 gcatacgggc tgcgcgaaca ccaccaaatc cgccgccgac aattaagacg ttatgagtag
     6301 cgggctgttc ggcggtgttc atctgatata atcatatgcg aaactaatct ggaatggcaa
     6361 gggacacttg aagaatcaga caatccgctg gctggctttg gggttggcca ttgccttggc
     6421 cttaccgatg atatggcaat ggtacagttt gagtgctgtg ccagcatcaa cctcatccga
     6481 gtatcttata gccattggac ggctggctgg catggtcatg accttcggtg tcttaatgca
     6541 gctgatttta atgtcgcgcc tgccgggcct tgaaaaggct gttggcttag aacggctggt
     6601 taccattcat
//
LOCUS       DASVGB010000097         8486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_738636, whole genome shotgun sequence.
ACCESSION   DASVGB010000097 DASVGB010000000
VERSION     DASVGB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738636"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..788)
                     /locus_tag="VF272_03010"
     CDS             complement(120..788)
                     /locus_tag="VF272_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259875.1"
                     /translation="MSLPADKTPPKPSKAIIVAVAIAALCLLAWVIVLVVGLYADSNP
                     TTAPAQPDTSEVLLETRCYFFKGVPAHYVETSQINLDDCLYDVELRGEISKAGKPIIQ
                     NNGELIGRIRVEPVRKTTELQWGKDLDEIVSYLEKSYYAPQGEILRLREPINLDGVTA
                     NKTTVKSTQQHIKTKDFVVVLKDPGYTLRSGAEATMFMITVTTPENNGEQILANIIKS
                     WRWR"
     gene            880..1791
                     /gene="cyoA"
                     /locus_tag="VF272_03015"
     CDS             880..1791
                     /gene="cyoA"
                     /locus_tag="VF272_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016211350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEX7259876.1"
                     /translation="MNSAVVKVLVVLLLIGGLVGGLVLFLAAHPVAVLDPRGLIASEQ
                     RNLIVVTTLLMLLIVVPVFGMTFGIAWKYRAGNTKARYTPDWDHSRVAETLWWGIPSV
                     IIVILAVITWKSSHDLDPFKPLISPNKPVTIQVVALPWKWLFIYPDQNIATVNYVQFP
                     ENTPIHFVITADAPMNSFWIPQLGGQIYAMPGMSTQLHLMASEVGSYAGSSANLSGAG
                     FANMRFEAKATTQVAFDDWVAATKKSPLHLGATDYNQLAQPSKRDDVAYYGIASNELY
                     DSIIMKYMLPADGTKHEHGAMQGMRAQ"
     gene            1788..3764
                     /gene="cyoB"
                     /locus_tag="VF272_03020"
     CDS             1788..3764
                     /gene="cyoB"
                     /locus_tag="VF272_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEX7259877.1"
                     /translation="MNLFGRLTLDAVQHDAIQTFGQLSVVMGGLTVVALLFYSKRWKW
                     LWREWLTSLDPKKIGVMYIVVAFVMLLRGVMDAAMMRTQQALAAGDAQGILSSEHFQQ
                     VFSAHGTIMIFFVAMGLMFGLINLIVPLQIGARDVAFPFLNSVSFWLFAVGMGLVNLS
                     LVVGEFAAAGWLAYPPLSGLEYSPGVGVDYWIWSLQIAGVGSLLAGINFLVTILKMRA
                     PGMTLMKMPLFTWSVLCSMILVVFAFPILTGTLGMLSLDRLMGMHFFTSDFGGNAMMY
                     INLIWAWGHPEVYILILPAFGIFSEVVATFSSKRLFGYVSMVWALGAITFLSFIVWLH
                     HFFTMGAGANVNAFFGIMTMVIAIPTGVKIFNWLFTMYRGRIRFTTPMLWFLGFVVTF
                     TIGGMAGVLMSVPAIDFQVHNSLFLVAHFHSMVVGGVLFGFFAGITYWFPKIFGFKLH
                     EKLGIYAFWCWIVGFLAAFMPLYILGLMGATRRLDHYDASLGWQWLFIVAGVGVAIIL
                     LGIGFQILQMIVSVLQRHKNRDRTGDPWGGRTLEWATSSPPPEYNFAITAQVHDRDAF
                     WAAKQAPLAVAKPVYHDIAVPRNTAMGMYIAGATFVIGFALIWHIWWLAALGLASMLL
                     LIVVRLSAENTERIITTDELAALEAGADKSGRTT"
     gene            3761..4345
                     /gene="cyoC"
                     /locus_tag="VF272_03025"
     CDS             3761..4345
                     /gene="cyoC"
                     /locus_tag="VF272_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEX7259878.1"
                     /translation="MTKQAPSVHKDDAKTLFGFWIYLMTDCILFASLFATYAVLHNNT
                     FGGPSGQELFSLPFVLSETIILLVSSFTCGLGLLAAHRGEKHQVIGWFIITFLLGSAF
                     LALELTEFTHLVNEGYSWQRSGFLSAFFTLVGTHGLHIATGLFWIVVMMIRVAKTGLT
                     VVNKRRLTVLSLFWHFLDIVWIFIFTIVYLMGAL"
     gene            4346..4699
                     /gene="cyoD"
                     /locus_tag="VF272_03030"
     CDS             4346..4699
                     /gene="cyoD"
                     /locus_tag="VF272_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018298931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEX7259879.1"
                     /translation="MGAGQGTLATYTAGFILSLILTLEAYALVAYQVLVGPALVAAIM
                     VTAVVQLLVQLVFFLHLGSESKPRWNMIVFLFMVMVLLILVLGSLWIMHNLNYHTMSP
                     SETDTHIIHDEGIQR"
     gene            4696..5556
                     /gene="cyoE"
                     /locus_tag="VF272_03035"
     CDS             4696..5556
                     /gene="cyoE"
                     /locus_tag="VF272_03035"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7259880.1"
                     /translation="MASLSTYYWLAKPGIVYGNCLAATAGFLLASKGHPSITLYIVMV
                     GGIALVMASGCVFNNYIDRGIDEKMARTKKRALVQGVLSATNALMYGTVLGIIGLAIL
                     LTGTNALTFGLALAGLVLYVGVYGYWKRRSVHGTLVGSLAGAIPPVVGYCAVTNRFDL
                     GALLLFLILVLWQMPHFYAIALYRFKDYKAADLPVLPVKQGMNAAKQQAIAYIIAFIL
                     VIPVLSVTGYTGYSFAIIMMVIGLVWLLKAIREYRTKDDAGWGRKLFGFSLIVMISLS
                     VMLSVGSMLP"
     gene            5574..5831
                     /locus_tag="VF272_03040"
     CDS             5574..5831
                     /locus_tag="VF272_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259881.1"
                     /translation="MVLLIHIAIALASLLYTTYVWLAPSRPKFTVGYTLVGLTLLSGT
                     YLTFISPAHMIQACTTGLIYIGIVSIGMVAAHGKLAHSKNH"
     gene            5947..6981
                     /locus_tag="VF272_03045"
     CDS             5947..6981
                     /locus_tag="VF272_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259882.1"
                     /translation="MSTKVGRSITATVLAGFLLALLIPSAAQAATVKPAQYNCPGTVQ
                     VQDLNLVNGEPKDASGVPVLKSPSALFDWLMTAAKQVNVGSPVPNLRPEVSSLKATLG
                     KFRRSVLVCVYPTAPDDALCPPRELLPPGSVFQWLLLTPALTVNHRCYELTPYSGTLS
                     TAKRSALLRRITSRIQRHLLLAVGCWVNGRVVYSGCGKARVATHYGLQADAAFLKWRH
                     CSSNPKRAYRQLGLTILNGVATVGVQFCDRKLQPQSWTVPLQRLPPRSPAGAAPVTPV
                     PQATTQPAPAPQPTVGPFTVAPEDQAPPIDTDNDNVADSIDQCDHDHGPLINNGCPLT
                     VPTPPPGPGR"
     gene            7182..8174
                     /locus_tag="VF272_03050"
     CDS             7182..8174
                     /locus_tag="VF272_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259883.1"
                     /translation="MPKTQHTSMPVSRTRRAGRTLGEWLSDALHAAFGAVLVGLLRMA
                     RPSVKNWRKIETPLLDLSYKPLRGKHIETFTADKPTDYWIAALFNNALIRLPKDDANQ
                     PFRRMGMAFAAVTALAISLIVLHYGSHTGVSYRELREYFPHVERDTYLMGLGIIKALS
                     VVLALFLWGIMVLMSYLAWTSRSWYIDSLEAELIKVDNKWVAHDFTVKMMWRRLHWKC
                     KETRLSHVDEAKADNDYLKKRYRGRNHIYLRLIDPRGEITKIRILKDIPAADHEPLTD
                     LLTQLGLLYKGGRFSAARQADKFARPEGPVRVSGEVVDEDDKDRRRDEYDRFRS"
BASE COUNT         1822 a         2275 c         2257 g         2132 t
ORIGIN      
        1 tgacccaaat ggcgttatcc gtaaggaata tgagcaggta acgccgaatg gccatggcga
       61 gcagatagta cgcgatctca gcgaactgca agctcagtaa aaggagtagc cgtcctgaac
      121 tagcgccacc gccaactctt aatgatgttt gctagtatct gttcgccgtt gttttccggc
      181 gtcgtaacgg taatcataaa catggttgcc tcggctccag aacgcagcgt atagccggga
      241 tctttcagta ccaccacaaa atccttggtt ttgatatgct gctgagtact ttttaccgtg
      301 gttttattag cagtaacccc gtccaaattg atcggctctc tcagccttag gatttcgccc
      361 tgcggcgcat aatagctttt ctccagatag ctgacaattt catccaggtc cttaccccat
      421 tgcagctcgg ttgtcttgcg aaccggctca actctgatcc ggccgataag ttcaccattg
      481 ttttgaatga tcggtttgcc ggctttgctt atttcgccgc gcaactcaac atcatacaaa
      541 caatcatcaa gattgatctg cgacgtctcc acataatgag ccggaactcc cttgaaaaag
      601 tagcagcgtg tctccagtag gacttctgat gtgtcgggtt gagccggagc agttgtcgga
      661 tttgaatcgg catacagccc gacaaccaac acaattaccc aagcaagcaa acacagtgcg
      721 gctatggcga ccgcaactat tatggccttg ctgggcttag ggggtgtttt atcagctggc
      781 agactcaccg gtgtgtctct ctattggttt atgtatcgcc attcttaact agtattgtaa
      841 tctataaccg tcgtaaaact ggtatagtag aagcataaga tgaatagcgc tgttgtgaaa
      901 gttttggtag tacttctgct tatcggcggt ctggtaggcg gacttgtgct tttcttagca
      961 gcccacccgg ttgccgtgtt agatccgaga ggattgattg ctagtgagca acgcaatctt
     1021 attgttgtta caacgctttt gatgctcctg attgttgtac cggtgtttgg tatgaccttc
     1081 ggcattgcct ggaagtaccg ggccggcaat accaaagccc gctacacccc ggactgggat
     1141 catagccggg ttgccgaaac cctgtggtgg ggaataccga gcgttattat tgtgatacta
     1201 gcagttatca cctggaaaag cagccatgat cttgacccgt tcaagccgtt gatatcacct
     1261 aataaaccgg taacgataca ggtagttgcg ctgccgtgga aatggctttt tatctatccc
     1321 gatcagaata ttgctaccgt taattatgtg cagttccctg aaaatacccc gatccatttc
     1381 gttattaccg ccgacgcccc aatgaactcc ttttggatcc cgcagttagg cggacaaatt
     1441 tatgccatgc ccggcatgag cactcagctg catctcatgg ccagcgaggt tggcagctat
     1501 gctggttctt cggccaactt aagcggtgcc ggtttcgcca atatgcgctt tgaagccaag
     1561 gcaacaacac aggtagcgtt tgacgattgg gttgccgcca cgaagaaatc accccttcac
     1621 ttaggtgcta cagactataa ccagctagct cagcctagca aaagggatga tgtagcatac
     1681 tacgggattg ccagcaacga actctatgac agcatcatca tgaagtacat gctgccagcc
     1741 gatggcacga agcatgagca tggagccatg cagggcatga gggcgcaatg aatctttttg
     1801 gacgcttaac cctcgatgcc gtacaacacg acgccattca aacctttggc caactgagcg
     1861 tagtcatggg cgggctgacg gttgttgcct tactgtttta ctctaagcgc tggaaatggc
     1921 tctggcgcga atggctgacc tcgcttgatc cgaaaaaaat cggcgttatg tacattgtgg
     1981 tggcctttgt aatgctgctg cgcggagtga tggatgccgc catgatgcgc acccagcaag
     2041 cgttagcagc aggcgatgct caaggaatct tatcttccga gcacttccaa caagtatttt
     2101 cagcccatgg caccatcatg attttctttg tcgcaatggg gctgatgttc ggcttaatca
     2161 acttgattgt gcctttgcaa atcggtgccc gcgacgtagc tttcccattc ttaaattcgg
     2221 tcagtttttg gctattcgca gtaggaatgg ggctggtaaa tctgtcactg gtggtagggg
     2281 agtttgccgc cgccggctgg ctggcctacc cgccgctttc cggacttgag tacagcccag
     2341 gagttggcgt cgattactgg atctggagtt tacagatagc cggggttggc agcttgctgg
     2401 cgggaattaa ctttttggta accattttaa agatgcgggc tcccggcatg acgctcatga
     2461 agatgccctt atttacctgg agtgtgctgt gcagcatgat cctagttgtc ttcgccttcc
     2521 caattttaac cggtacgctg ggtatgcttt cgcttgaccg cttgatgggc atgcatttct
     2581 ttacatcgga ttttggcggc aacgccatga tgtacatcaa tctgatttgg gcttgggggc
     2641 atccagaggt ttacattttg attttgccgg ccttcggcat tttttccgaa gtggttgcca
     2701 cgttctccag caagcggctg tttggctatg tctcaatggt gtgggcgctc ggagccatca
     2761 cctttttgtc gttcatcgtc tggctgcacc acttctttac tatgggcgcc ggcgccaatg
     2821 taaatgcctt cttcggcatt atgacgatgg tgattgcaat tccaaccggc gtcaaaatat
     2881 tcaactggct atttacgatg tatcgtgggc gcatccgctt taccactccg atgctgtggt
     2941 tcctcggatt tgtggtgacc tttacgattg gcggcatggc cggggtgctc atgtcggttc
     3001 cggctatcga ttttcaagtc cataacagcc ttttcttggt agcccacttc cattctatgg
     3061 tggttggcgg ggtactgttc ggcttttttg ccggtattac gtactggttt ccgaaaattt
     3121 tcggtttcaa gttgcatgaa aagctgggca tctatgcatt ctggtgctgg atcgtcggct
     3181 ttttggccgc ttttatgcca ctgtacatct taggactgat gggtgccacc cgccggttgg
     3241 accactacga cgcctcgcta gggtggcagt ggttgtttat tgtggccggt gttggcgttg
     3301 cgattatttt attgggcatc ggtttccaga tactgcaaat gatagtgagc gttttgcagc
     3361 gccacaaaaa ccgcgaccgt accggcgacc cctggggcgg ccgtaccctc gaatgggcca
     3421 ccagctcgcc cccgcctgaa tataactttg ccatcaccgc tcaagtacat gaccgcgatg
     3481 ctttttgggc ggccaagcag gcacctcttg cagtcgccaa gccggtttac cacgatatcg
     3541 cggtaccgcg caataccgca atgggaatgt atatcgccgg tgcaaccttc gtgattggtt
     3601 tcgccctcat atggcatatc tggtggctgg cagcgcttgg tctagccagt atgctgctgc
     3661 tcattgtggt acggctatca gctgaaaaca ccgagcgcat catcaccact gacgagcttg
     3721 ccgcactgga ggccggagca gacaagagcg ggaggacgac atgacgaaac aggcaccctc
     3781 agtgcataag gatgacgcca aaacgctgtt cgggttttgg atttacctga tgaccgactg
     3841 tattttattt gccagcttgt ttgccaccta cgccgtttta cacaacaaca cctttggcgg
     3901 gccgtcgggg caggagctgt tcagcttgcc gtttgtactg agcgaaacga ttatcttgct
     3961 cgtcagcagc tttacctgcg ggctgggctt actggccgcc caccgaggcg aaaaacatca
     4021 ggttatcggc tggtttatta ttacctttct gctgggttca gccttcttgg ctctggagct
     4081 aaccgagttt acccatttgg taaacgaagg ctatagctgg cagcgcagcg gctttttatc
     4141 ggcctttttc acccttgtcg gtacgcatgg gctgcatata gcgaccggac tattctggat
     4201 tgtcgtcatg atgatcaggg tagccaaaac cggcttaacc gtcgtaaaca aacgccgact
     4261 gacagtcctg agcctatttt ggcacttttt agatattgtc tggattttca tcttcaccat
     4321 cgtgtactta atgggggcac tttaaatggg cgccggacag ggaacccttg caacctacac
     4381 agccgggttt atcctatcgc tgatattgac cctagaagcc tatgccttgg tggcttacca
     4441 agtcttggtt ggcccggcgc tggtagcagc catcatggtt accgccgtgg tgcagctact
     4501 agtgcaactg gtattctttc ttcaccttgg cagcgaatcc aaaccgcgct ggaacatgat
     4561 tgtgtttctg tttatggtga tggttttgct gatcctggtt ttaggatcgc tctggattat
     4621 gcacaacctt aattaccaca ccatgtcgcc atctgaaacc gacacccata tcatccacga
     4681 cgaggggatt caacgatagc atcgcttagc acctactact ggctggcaaa gccggggatt
     4741 gtatatggta actgcttagc cgcgacggcc ggatttttat tagcctcgaa gggacacccc
     4801 tcgattaccc tgtatatcgt aatggtaggc ggcatcgcct tggttatggc ttcgggctgc
     4861 gttttcaata attatattga ccgcggtatt gatgagaaga tggctcgaac caagaaacgg
     4921 gcactggtac agggagtttt gagtgctacc aatgccctga tgtatggcac ggttctggga
     4981 attattgggc tggcgatttt gctgacaggc accaacgcat taacctttgg actggctctg
     5041 gccggattgg tgctgtatgt gggcgtatac gggtattgga agcgccgctc ggttcacggc
     5101 acccttgttg gcagcctagc tggagcaatt ccgccggtgg tgggttactg cgccgtcacc
     5161 aatcgctttg acctgggggc gctgctgcta tttctaatct tagtactctg gcaaatgccc
     5221 catttttatg cgattgcact gtaccgtttc aaggattata aggccgctga cctcccggtt
     5281 ttgccagtga aacaggggat gaacgctgcg aaacaacaag cgattgccta tattattgcc
     5341 tttattctgg ttattccagt actgagtgtt accggctaca ccggctatag ctttgccatc
     5401 attatgatgg taattggcct ggtttggctg ctgaaggcca tacgagaata tagaacgaaa
     5461 gacgatgccg gatggggccg taaactgttt gggttttcac tgatcgtaat gatctcctta
     5521 tcggtaatgc tatctgttgg tagtatgctg ccataagtgt tatagtagtg cttatggtac
     5581 ttcttattca cattgcaatc gcactggcaa gcctgcttta caccacctat gtatggctgg
     5641 ctccctcgcg cccaaaattt acggttggtt acacgctggt cggacttacc ttgctaagcg
     5701 gaacctatct gacattcatt agcccggctc atatgatcca agcctgtacc accggactta
     5761 tctacattgg catcgtgagc attggcatgg ttgctgccca cggcaagctg gctcacagca
     5821 aaaatcacta attccatctg gtaaaccctt tactttttag ctcatatatg ggatattatc
     5881 gccaggtttg agtacccata caatctggtc aacatctcac gaccgaccct tcgaaagggg
     5941 aggaccatgt ccaccaaggt agggcgaagt attacagcaa cggtgctggc aggatttctg
     6001 ctggcattgc tgatcccgtc ggcggcacag gccgccaccg tgaagcccgc gcagtacaac
     6061 tgcccgggta ccgtccaggt gcaggatctg aacctggtca atggcgagcc gaaggatgcc
     6121 agtggcgtgc ccgtgctgaa gagcccgagc gcgttgtttg actggctgat gacggccgcc
     6181 aagcaggtca atgtcggttc gccggtgcca aacctccgcc cggaggtttc gtcgctgaag
     6241 gccacgctgg ggaagttccg acgcagcgtt ctggtgtgcg tctacccgac cgccccagac
     6301 gatgcgctgt gtccgccccg ggaactgctg ccgcctggca gcgtgttcca gtggctgctc
     6361 ctgaccccgg cgctgacggt caatcaccgg tgctatgagc tgacgcccta cagcggcact
     6421 ctgagcacgg caaagcgcag tgcgctgctg cggcgcatca cctcgcgcat tcagcgccat
     6481 ctgctgctgg cggttggctg ctgggtcaac gggcgcgtag tgtacagcgg ttgcgggaag
     6541 gcccgggtgg ccacccacta cggtctgcag gccgacgcgg ccttcctgaa gtggcgccac
     6601 tgcagctcca accccaagcg ggcgtatcgg cagctgggtc tgaccatcct gaacggtgtc
     6661 gccacggtag gcgtgcagtt ctgcgaccgg aagctccagc cgcaaagctg gaccgttccg
     6721 ctgcaacggc tgcccccgcg gtcacctgct ggtgcggctc cggtgactcc ggtgccgcag
     6781 gcgacaaccc agccggcgcc ggctccacag ccgacagttg gaccgttcac ggttgcgccc
     6841 gaggaccagg ctccgccgat cgacaccgac aacgacaacg tcgctgacag tattgatcag
     6901 tgcgaccacg accatggccc gctcatcaac aacggctgtc cgctcaccgt cccgacgcca
     6961 ccgccgggac ctggccgctg agcgctcaac tcaaccaccg aaccctcggg acgacgtact
     7021 gtcccggggg ttcgctgccg tttacggaat agcgacagga gcaattatct tgattaatta
     7081 atgaaattat ttaatactta tacggtattt tgttttgccc atctcaagac gaagcagacg
     7141 cacctcacat ccatgccaat cgctcgagga gagtgatcgt tatgccgaag acccagcata
     7201 cttcaatgcc ggtcagccgc acccggcgtg ccggccgtac cctaggggaa tggctgtccg
     7261 atgcgctgca cgctgcgttc ggggcagttc tggtggggct ccttcgcatg gcgcgaccgt
     7321 cagtaaagaa ctggcgcaag atcgagacgc cccttcttga tctgagctac aagccgctac
     7381 gcggcaagca catcgagacg tttaccgccg acaagccaac tgactactgg atcgccgcgc
     7441 ttttcaacaa cgcgctcatt cgccttccga aggacgacgc gaatcagccc ttccggcgaa
     7501 tgggaatggc ttttgctgcc gtaaccgctc tggcaatcag cctgatcgtg ctgcactacg
     7561 gctcccatac cggcgtcagt tatcgcgagc tccgcgagta cttcccccac gtcgagcgcg
     7621 acacctacct gatggggctg ggaatcatca aggcgctcag cgtcgtactg gcgctgtttc
     7681 tctgggggat catggtcctc atgagctacc tcgcctggac ctcgcgatcc tggtatatcg
     7741 actcgctcga ggccgagctc atcaaggtcg acaacaagtg ggttgcccac gacttcacgg
     7801 tcaagatgat gtggcgcagg ctccactgga aatgcaagga aacccgcttg agccacgtcg
     7861 acgaggccaa ggcagacaac gactacctca agaagcgcta ccgaggccgc aaccacatct
     7921 atctcagact gatcgacccg cggggagaaa tcaccaagat tcggatcctg aaggacatcc
     7981 ccgcagccga ccatgaaccg ctcaccgatc tgctgactca actgggactc ttgtacaaag
     8041 gaggccggtt cagcgcagcc cgccaagccg acaagtttgc ccggccggag ggccctgttc
     8101 gggtttccgg cgaggtggtc gacgaggacg acaaggatcg taggagggat gaatacgacc
     8161 gcttccgttc gtgacatgca actgcagggc ggccttcggg ccgccctgca gaggattttg
     8221 ccattataat aacgcttgtg ttttaagcct tttacagata agaaaacgtt gacaaaccaa
     8281 ccgaatttac tactattata taacgaatag aaaaggtgga agatgctcaa agcagcaggg
     8341 aataccattt acggtttatg acctagccac taagggtcat aaaactactc gacccgacac
     8401 tttaattaca tacatttgat tgaagcgcca ggttgggcag gcgatcgtta acggcctacc
     8461 caaaaatacg accgattggg cgtgtc
//
LOCUS       DASVGB010000098         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_629698, whole genome shotgun sequence.
ACCESSION   DASVGB010000098 DASVGB010000000
VERSION     DASVGB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629698"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..535
                     /locus_tag="VF272_03055"
     CDS             158..535
                     /locus_tag="VF272_03055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7259884.1"
                     /translation="MDQLKLFVRSLYTAFTGLVFVVRVERNARLHLLIAIAALGLSVV
                     LGVTNTELAAIFFAIILVFTTEVINTALEKTLDLIEQKSHAQVRIIKDIAAGAVLVTS
                     IGALAIGAAIFLPYIGNLLWPGR"
     gene            520..1284
                     /gene="map"
                     /locus_tag="VF272_03060"
     CDS             520..1284
                     /gene="map"
                     /locus_tag="VF272_03060"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7259885.1"
                     /translation="MAGTLNGASIKTASEIEAMRTGGKMLAQVLAFVGSEMAAGMTTD
                     ELDQLAASEVKRLGGEAAFLGHEGFPKSICISVNDEVVHGIPGSRVLQTGDVVGLDFG
                     VRYDGLVTDSAVTLPIGSMKAPARRLLNATEAALMIGIDQATAGSRVGDISSAIQRHL
                     QTANLGIIRELAGHGVGRELWEEPQIPNYGRAGAGPVLKAGMTVAIEPMATLGSPEIT
                     ILGDKWTIAARDGSLAAQFEHTILITNGAPEILTLA"
     gene            1446..2678
                     /gene="ftsA"
                     /locus_tag="VF272_03065"
     CDS             1446..2678
                     /gene="ftsA"
                     /locus_tag="VF272_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7259886.1"
                     /translation="MAIRQQDDIFIGLDVGSTKVCCIVGLQEESAPHPSIIGVGMAPV
                     GGMRRGVVVDVEETVSSITAAVDEAERLSGVAIDRATINIDGAHVSSLNSSGVIAVGR
                     ADHEITAEDVRRVEEAATAIQLPPNREIIQIFPRSFTVDGQTNIKDPVGMNGVRLEVE
                     AHIVTGATPAIKNLQRAVYQAGIDIEGQIMVPLAAARAVLSKKQLELGVALVDIGGGT
                     TGIAVYEEGEILYSSVLPVGAGHVTNDLAIGLRTDIEIAETIKLKYITAGIGKAGKSD
                     KIKIEELGDDGMVARRDLYDISSARLEEIFQMVQAEFAKIGKSKLLPGGVVLTGGGAK
                     MPGIEELAKEVLGLPVTIGRPSDLSGLVDKVSDPAFAAPVGLMFENMSRTGMSDQGND
                     RLGDTVDRIRKTLRNLLP"
BASE COUNT          658 a          642 c          778 g          701 t
ORIGIN      
        1 ctaaaacgcc cttagcagac gagattgccc tcctggcggc tcatggcatc ctgcatgcgc
       61 tcggctatga tcatgcggca tcagaagacc gagacaagct tgataaactg caagctgata
      121 ttatgggtgc agccggatta acctatcggg attttcaatg gatcaactaa aactctttgt
      181 tcgcagcctc tatactgcct ttaccggtct ggtttttgtc gtccgggttg agcgtaacgc
      241 tcgacttcat cttttaatcg cgattgccgc tctgggctta agcgtggtgt tgggtgtcac
      301 caacaccgaa ctcgcagcga tcttcttcgc catcatcctg gtatttacga ctgaagtgat
      361 aaatactgct ctggaaaaaa cccttgattt gatcgagcaa aaatctcatg ctcaagttcg
      421 gattattaag gatatcgccg ccggggccgt attggtaaca tcgattgggg cgctggcaat
      481 tggagcggct attttcttac cctatattgg aaacctttta tggccgggac gctaaacggc
      541 gcgagtatca aaaccgcgtc cgagattgag gcaatgcgga cgggcggtaa gatgctggcc
      601 caagtattag cctttgtggg atcagaaatg gcggctggga tgactaccga cgaactagat
      661 cagctggccg ccagcgaggt taagcgcctt ggaggagagg ctgcctttct tgggcatgag
      721 ggctttccta aatccatctg catatcagtg aatgacgaag tagtgcatgg catccctggg
      781 agccgagtgc tgcagacggg agacgtcgtt ggcttggatt tcggcgttcg ttatgacggc
      841 ttggtgacag attcggcagt tactctgcca attggcagta tgaaggctcc ggctcggcga
      901 ctgctgaacg caaccgaagc tgcattaatg atcgggattg atcaggctac tgcaggaagt
      961 cgggtcgggg atatttcctc tgctatccaa cgtcatctgc aaacagcaaa cttgggtatt
     1021 attcgcgagc tagctggtca tggggtaggc cgtgaactct gggaggagcc tcaaatccct
     1081 aattatggcc gcgccggcgc cgggcctgta ttaaaggccg gaatgacggt tgcgattgaa
     1141 cctatggcaa ccttgggttc tccggaaata acaattctcg gtgataaatg gacgatcgct
     1201 gcccgtgacg gttcgctggc agctcagttt gaacatacga ttttaattac gaatggcgca
     1261 ccggagatcc ttaccctagc ctaaactcat ttgtgtatac tacatttgtg ttcaggccgt
     1321 ttgcacactg tagccaatga gctaatgtgt tcggcctcgt agcacaaata gtgttcaagg
     1381 ctcgcttgcg taaagattag cataagcact ataatagaga ttaagataac aagcgggaat
     1441 tttacgtggc cattcgccag caggatgaca tttttatcgg cttagatgtc ggcagcacca
     1501 aggtttgttg catcgtcggc cttcaagagg agtcggctcc gcaccctagt attattgggg
     1561 tcggtatggc accggttgga ggtatgcgcc ggggtgttgt ggttgatgtc gaagaaaccg
     1621 tatcatccat aacggcggcg gttgatgagg cggagcgatt aagcggcgtg gctatcgacc
     1681 gggctacgat taatattgat ggtgcgcacg tttctagctt gaactccagc ggggttattg
     1741 ccgtcggccg ggccgaccat gagattactg ccgaagatgt ccgccgagta gaagaagccg
     1801 ctaccgcgat tcagctcccg ccaaaccgtg aaattattca aatattcccg cgttccttta
     1861 ccgttgacgg acagactaat ataaaagatc cggttggcat gaatggcgta cggctggagg
     1921 ttgaggctca catcgtaacc ggcgcaacac cggctattaa gaatctgcaa cgagcggtct
     1981 atcaagccgg gattgatatt gaaggacaga tcatggttcc tttagctgct gctcgggccg
     2041 tattatccaa gaagcagctt gagctgggtg ttgcacttgt agatatcggc ggcggtacca
     2101 ccggaattgc ggtctatgag gaaggtgaga ttttgtactc cagcgtcttg ccggtaggtg
     2161 ccggccacgt aaccaatgat ttagcgattg gtctacgaac cgatattgag attgctgaaa
     2221 ccattaagct taaatatatt acggccggaa tcggaaaagc cggaaagagc gataagataa
     2281 agattgaaga attgggcgat gacggcatgg ttgcccgccg tgacctttat gatattagca
     2341 gcgcccgctt agaggaaatc tttcaaatgg ttcaggccga gtttgctaaa atcggtaaaa
     2401 gcaaactact gcccgggggg gtagtcttaa ccggcggcgg tgctaaaatg ccgggcattg
     2461 aagagcttgc taaggaggtg cttgggctac cggttacgat tggccggccc agtgatttga
     2521 gcggcttggt tgataaagta agcgatccgg ctttcgcagc tccggttggt cttatgttcg
     2581 aaaatatgtc tcgtaccgga atgagtgacc aaggaaatga tcgccttggc gacactgtcg
     2641 atcgtattcg caaaacgctg cgcaacctct tgccctagta ctcattggta cctgcatccg
     2701 tccattctta gccgccgcat atcgcttcat tgttgaacag aatacatagt cattcagtag
     2761 agtagttggt gcgctcccg
//
LOCUS       DASVGB010000099         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1203122, whole genome shotgun sequence.
ACCESSION   DASVGB010000099 DASVGB010000000
VERSION     DASVGB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203122"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="VF272_03070"
     CDS             complement(<1..1714)
                     /locus_tag="VF272_03070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7259887.1"
                     /translation="MNNDANLNEISEVQGATPDFKAELASQLQELVPEAITDGKVDVT
                     KLNELLSDDTADESERFGLFWPGKKRAMRAAQESTPATLKPAMNESKDWETTENVFIE
                     GDNLEVLKVMQKHYHNSVKMIYIDPPYNTGKDFVYPDNYIEGLQSYLEYTKQLDEEGH
                     KVSTNSETDGRYHSNWLNMMYPRLKLGRNLLTGDGVMYISIDDHEAENLKKMCHEIFG
                     ENNFLAQIVWANKEGGGGSDSTTFKVKHEYILAYAKNKDKVRIYGEEVEDDSSYPHSD
                     EFVAERGKYKLIKLNSFSIQYSASLDYPIAAPDGAEVVPEEDGRRAIWRWSRKKYDWG
                     VENGFIVHKQKDDGRWIIYTKQYYRVDNDNQPIVRTLPPFALIEKYSSTGATKGITEL
                     FGKTKLFDYPKPFELIVKLAEWVTKDNDLVLDFFSGSGTTAHAIMKLNANDGGTRRNI
                     QVQLPEPSKPDSEAYKAGYKNIADLAKERVRLAGEKVKADVTKPLDVGFRAYKLADTN
                     FTKWSTSSSVDVDALQQHLLTIRESSNDDATQEDLLAELLLKLGVSLTARAEKKEVGG
                     LPVWS"
     gene            complement(1852..1926)
                     /locus_tag="VF272_03075"
     tRNA            complement(1852..1926)
                     /locus_tag="VF272_03075"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1892..1894),aa:Gln,seq:ttg)
     gene            2022..>2583
                     /locus_tag="VF272_03080"
     CDS             2022..>2583
                     /locus_tag="VF272_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7259888.1"
                     /translation="MERKRVTVAIETSTIVRVLLLVLAFVGSIWLVTRVATPLVWVLV
                     AIFLAVSLDPAVNGLMKALKVSRLWATGIVFALFVGFVSFMSASLVPPLVNQTQAFAT
                     QLPGLVQQAENSDTYLSRVISDYDLVNRAHDVQDDLIANITTGNGTALGIVVSIFSGL
                     AAALTILTFTFFMLLEGPHWVRVLGNY"
BASE COUNT          596 a          636 c          602 g          749 t
ORIGIN      
        1 cgctccagac tggcaaacca cctacttcct tcttttcagc tctggctgtc agcgaaacac
       61 ccagcttgag taatagctcc gcgagtaaat cctcttgagt ggcatcatca ttactgcttt
      121 cacgtatggt cagtaagtgc tgctgcaagg cgtcaacatc cacgctactg ctggtgctcc
      181 acttagtgaa gttggtgtcg gctagcttgt aggcccgaaa accaacatca agcggctttg
      241 taacatcagc cttgaccttt tcaccggcca atcgcacgcg ctctttcgca agatcagcaa
      301 tgttcttata accggccttg taggcctcgg aatctggttt tgagggctca ggcagctgaa
      361 cttgaatgtt cctgcgggtt cccccatcat tggcatttaa cttcataatg gcatgtgccg
      421 tagtgccact accagaaaag aaatcaagta ccaggtcatt atccttggtg acccactcag
      481 ccagcttgac aatcagctca aagggctttg gataatcaaa caacttggtt ttaccaaaca
      541 gctcggtaat gcccttggtc gctccggttg aagagtactt ttcaattaag gcaaagggag
      601 gcaacgtccg aacaattggc tgattgtcat tatcaaccct atagtattgc ttggtataaa
      661 tgatccaacg cccatcatct ttctgtttgt gcacgataaa gccattctca acgccccagt
      721 catatttctt acgagaccag cgccaaatag cccgccgtcc gtcctcttct ggcacaactt
      781 ccgctccatc gggcgctgca attgggtaat ccaggctggc tgagtactga atgctgaaac
      841 tattgagttt aatcaactta tatttgccgc gttctgcaac aaattcatcg gaatgaggat
      901 aggagctatc atcttcaacc tcttcaccgt aaatgcggac tttgtctttg ttcttggcat
      961 aagccaggat atattcatgc ttcactttaa aagtcgtgct atcggatccg ccgccgcctt
     1021 ccttgttagc ccagacgatt tgagcgagaa agttattttc accaaagatt tcatggcaca
     1081 tctttttaag gttttcggct tcatgatcat caatgctgat gtacatcacg ccatcgccag
     1141 tcagcagatt tcgccccagc tttagccgcg ggtacatcat gttgagccag ttgctgtgat
     1201 agcgtccgtc ggtttcgcta ttggtgctaa ccttatgacc ttcttcgtcg agctgcttgg
     1261 tatactccaa ataactctgt agcccttcta tatagttatc ggggtacaca aaatccttgc
     1321 cggtattgta gggtggatca atataaatca ttttgaccga gttgtgataa tgcttttgca
     1381 tcacctttaa gacttcaaga ttatcgcctt caataaagac gttctcggta gtctcccagt
     1441 ccttgctctc attcattgcc ggcttcaaag tagctggagt tgattcttga gctgcccgca
     1501 tagcgcgctt tttgcccggc cagaacaacc caaaccgctc actttcgtca gcagtatcat
     1561 cgctcagcag ctcattgagc ttcgtaacat caaccttacc atctgttatc gcctccggaa
     1621 ccagttcttg gagttgcgag gcaagctcgg ccttgaagtc gggggtagct ccctgaactt
     1681 ccgatatttc gtttaaatta gcgtcattat tcatataagt atcgttctag tatatcgggt
     1741 tgagaaaatg tgtctatttg ggtgagcagg tttctttagt ctttttctta tgtacgtaca
     1801 taacagaagg agaggcatta aggtgtcatg cttttgtcga ccgcaaactc ttggctgggg
     1861 cggagggatt cgaacccccg aatgccagga ccaaaacctg gtgccttacc gcttggccac
     1921 gccccattgc gtgctaactc atagtattgt actaagttac accttagggc gctaggcatg
     1981 taaggttgaa aggcataagc agttcaggta gagtgtaaga tatggagcgc aaacgagtca
     2041 ccgtcgccat cgaaaccagc actattgtgc gggttttact gttagtgttg gcttttgtag
     2101 gcagtatttg gctggttacc agggtggcta cgccactcgt atgggtgctt gtggccatat
     2161 tcctagccgt ttcgctggat ccggcggtaa atggcttaat gaaggcgctg aaagttagcc
     2221 ggctgtgggc tacaggaatc gtgttcgcgc tatttgtagg ctttgtcagc tttatgagcg
     2281 ctagtttagt gccgccatta gtaaatcaaa cccaggcttt tgcaacgcag ctccctgggc
     2341 ttgtgcagca ggcggaaaac tccgacacct acctttcccg ggttatttcg gattatgatc
     2401 tggtcaaccg cgcacatgat gtgcaagacg acttgattgc caacattaca accgggaatg
     2461 gtacggcatt gggcattgtt gtcagcattt ttagcggact tgcggctgca ctgaccattc
     2521 tgacctttac gttctttatg ttgttggaag gtccacattg ggttcgtgtg ttgggaaact
     2581 atg
//
LOCUS       DASVGB010000100         8666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_914523, whole genome shotgun sequence.
ACCESSION   DASVGB010000100 DASVGB010000000
VERSION     DASVGB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914523"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1076)
                     /locus_tag="VF272_03085"
     CDS             complement(102..1076)
                     /locus_tag="VF272_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7259889.1"
                     /translation="MEKPTPIIVSGSIAIDRIMSFTGRYREIIRPEKLESLSVSVLLD
                     SLNDSDGGVGANIAYTLALLGDSPILLGSAGKDATAYLEKLAHAGVTITAVHTSDLHT
                     ATYTVFTDSEENQVAGFYPGAMSDSESLSIEPWKDQSPLMLVSPHDPAAMARQVRECQ
                     KWNIRLCYDVGQQVTSLPAADMKEGIKAASIVIVNEYEMGMLAARLETDADTIKKSVP
                     VVITTFGKGGSVIEGSAVPEPLNIEIVKATQVVDPTGAGDCYRAGFLYGYSRGWDLRI
                     CGQLGATCATYAIEQTGTQNHSFSFKEVAERYQKNFTQALPPTQNATH"
     gene            1166..1750
                     /locus_tag="VF272_03090"
     CDS             1166..1750
                     /locus_tag="VF272_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259890.1"
                     /translation="MTKNDLKTIITPSFSQILLCLLISICITSIAYWETLINILARNT
                     LTSTTSLSASFIGYLSSFGKLPFTGNLFIILFWGILAILIYCIGLVLANVLIDVRNSV
                     VVKTRFTKPAGGRNVVGRLMVMAIQVVAVVVFIGVLVACGLLVFPLFIQIAGMAILSG
                     LTLASSVLVVGAICGTALALYVLWTLAQLVFTLL"
     gene            1859..3430
                     /locus_tag="VF272_03095"
     CDS             1859..3430
                     /locus_tag="VF272_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259891.1"
                     /translation="MQPLALSAEGQRLIHGLTAANTAEHKPDYDKVHISGVGGQLYFA
                     YEQLRNAAEYGERHLLVRRAIERFLYRNWQLHDRQPTNEMPLELVSELTHARYLKNDS
                     IPRSSVRTIEEIIQRYTKLAEAIRQHHKVPGDRLKVWVFQSASVEIEHQLVSHTQTDA
                     FIDFVYHHYKQHIDPKIFDQVNEHTTEVVLYCAVHRTLFKSDIATTRYYALAARLHHD
                     GADTLAYFVTLNQAVDRLYQAPLTNRVGRLANRSGGPMRILREVVMSTNAPEQLLSKH
                     DELMSRVAHEAGQQYKLTRKRLNEGIVRSVIFLFITKVLLGVAIEVPYDLLVVGAVAW
                     TPLIMNTLFPPLYMATIGWGIRTPGRRNTEIIQSQLSRILFQTDDPPIWYRVRKRVTS
                     SGLNTAFNFVYAVAFLLSFGVLIYLLSSLGFNIVNGAIFFIFLSIVSFFGFRLLESAR
                     ELEVVDQRKSLFSVALDFFYTPFIRVGHWLTDKYSRLNVVTFVMDLAIELPLKTSLRV
                     VRQWAGFIRDKQEEL"
     gene            complement(3427..4374)
                     /locus_tag="VF272_03100"
     CDS             complement(3427..4374)
                     /locus_tag="VF272_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7259892.1"
                     /translation="MKLFSNAQFSRRQQAELLLSLGVGVLIAGIGISIILLVIKLVYQ
                     LSAMPWSTYLLIIAGIEGIVLIAALLFLLQVMRRDIELEHSKEVFIALASHQLRTHPT
                     AIKNFTSMLLGNYTGKLSAKQRKYLEMLNTSNESQLRIIEGLLCVAELESKQLKLLPE
                     PVEVVALLHEVLTELQPRLTENQQHLELEIPDKLTTITDRRCLRTAMENLIANATKYT
                     PSKGTIHIQLGQSKGHIILAVTDTGVGIADKDLPRLFVKFSRINNALSIKVAGTGLGL
                     YIVKTIARLLHGSITVTSKYGTGSTFTLRISKTLSARWP"
     gene            complement(4384..5643)
                     /locus_tag="VF272_03105"
     CDS             complement(4384..5643)
                     /locus_tag="VF272_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7259893.1"
                     /translation="MSQLIRFEKIVPEGKAMGRLADGRAVFAIGPLPGELARVGIRKQ
                     RKRYAEAVLHDIIEPSAKRNLIAEDHCLVCSPWQGVEYTYQLELKRAMLGDAYAQQHL
                     SVPINEFVPSPARLGYRNRLDFSVARIDGQLQLAFHARGSWDEFIALPEGCALGDSSM
                     NQAARNLLIQLNDGDFQVEPATITVRYAPSTGQMLTILTTAVNASWKTITAKKLGSFL
                     VASPLPGSGASGKIEYQSGNSYVTETIAGVDLAYPHSAFFQSNIPVFEKSLEYITKVI
                     VPSSRIIELYSGVGAIGLPLAAQGVSVQGIEVHTEAVAFAERNARANAISSYSAEAVA
                     AEHMNSTLLNNVDCVIVDPPRAGLHPRVIRWLLEAHPKQIIYLSCNPVTQARDIARLL
                     PTYRCTSLIGFDFYPGTLHLEALAVLS"
     gene            5700..6923
                     /gene="tgt"
                     /locus_tag="VF272_03110"
     CDS             5700..6923
                     /gene="tgt"
                     /locus_tag="VF272_03110"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7259894.1"
                     /translation="MAFSFHIHQTDGASRRGTITTPHGPIETPAFIPVGTQAAVKTLD
                     AHDLQQLKLPAVLANTYHLYLRPGVEIVERHGGLHAFMQWDGPTFTDSGGFQVFSLGF
                     GLSHGVGKISTIFPDEAAASQAKAVSKPKLMKVDDDGVSFRSHIDGSPHRFTAESSIG
                     LQQQIGADIILAFDECTSPLHDERYTVRALERTHAWAKRSLSAWSNRSEQALYGIVQG
                     GAYKTLRLESAAFINNLDFPGHAIGGSLGRSKQDMYDILEWTTPQLDARKPRHLLGIG
                     EIEDLFAGAARGMDTFDCVAPTRIARNGAVYISPAAGGSLATRFRLNISAAQYADDSN
                     PIDPTCECQVCQTYSRAYLRHLFNTHELLSMRLATIHNLYFVGRLMEHIRKAIEQREL
                     TELAKQWGVARLLPH"
     gene            6942..7424
                     /locus_tag="VF272_03115"
     CDS             6942..7424
                     /locus_tag="VF272_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX7259895.1"
                     /translation="MSRKQHHRSYILQTIQPGLVGLMDGSVSTLAPIFAAAFATQDSH
                     IAFLVGLSASTGAGISMAFAEALSDDGKISGRGSPWRRGVIMGVMTFLGGIFHTLPFL
                     LSDIHVALIAAYAVVGVELVTIAYIRYRYFSMSFWYSCLQVIVGGALVFAAGVLIGES
                     "
     gene            complement(7421..7798)
                     /locus_tag="VF272_03120"
     CDS             complement(7421..7798)
                     /locus_tag="VF272_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259896.1"
                     /translation="MLLKNGPSMISTSFITFAEQFSATHPVIEVTGEYYELPDRVVYT
                     SAAHPLEGGGRVEVNTTTADTPPDFEWKHEVTINDKQRKQFIHLIVRHDDSVVETYGK
                     TILPVNTERAIEIMAVIDELLNA"
     gene            7894..8475
                     /locus_tag="VF272_03125"
     CDS             7894..8475
                     /locus_tag="VF272_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259897.1"
                     /translation="MNISHFSQQHPYAYRRLLVIASIAMTLSGLQSMLTADKPVVFAV
                     GSLALSVPHAPAGNTQEKPKTVSQPTSFGGLTRLPGKDGSGTQPAPAMLASASSGVGN
                     DMRQLGRIMSAARFGDSHWPALNNLWTRESNWNPAARNPSSGACGIPQALPCSKIPDM
                     SPAGQLTWGLTYIERRYGNPSNAWQFWLSHHWY"
BASE COUNT         2005 a         2086 c         2231 g         2344 t
ORIGIN      
        1 tctttttcag caaggatgat ttcgcgacga ccccattcgg ccaaactgat atcggctact
       61 ttgtaatcgg ttgaattgat agtcttggcc atatgattct cctaatgtgt tgcgttttgt
      121 gttgggggta atgcttgtgt aaagtttttc tgatatcgct ctgcgacttc cttaaagctg
      181 aaactatgat tttgggtgcc ggtttgttcg atcgcgtagg tagcacaggt cgcaccaagt
      241 tggccgcaga tacgcaagtc ccaaccgcgg ctatagccat ataaaaatcc tgcgcggtag
      301 caatcgccgg ccccggttgg atctaccact tgtgttgctt tcacaatttc aatattcaac
      361 ggctctggaa ccgcactgcc ttcaataacc gagccgccct ttccaaacgt ggtaattact
      421 accggcacgc tttttttgat tgtgtcggca tcggtttcga gccgggcggc cagcatgccc
      481 atttcatact catttactat aacaattgag gctgccttaa tcccttcctt catatctgca
      541 gccggtaagc ttgtgacctg ctggccgaca tcgtagcaca agcggatgtt ccatttttgg
      601 cactcacgca cttgtcgagc catcgcagcc ggatcatgcg gggatacgag catcagcgga
      661 ctctggtctt tccatggttc gatgcttaat gactcactgt cagacatggc gccggggtaa
      721 aaaccggcga cttgattctc ttcgctgtcg gtaaacaccg tataggtagc ggtgtgtagg
      781 tcgctggtat gcacggcagt aatagtaact cctgcgtgcg ctagtttttc taaataagct
      841 gtagcatcct tgccagccga tccaagcaaa ataggggagt cccctaggag cgcgagagta
      901 taggcgatgt ttgcgccaac gccgccatcg ctgtcgttca ggctgtcaag caggactgat
      961 accgagaggc tttcgagttt ttccggacga ataatctcgc ggtagcggcc ggtaaagctc
     1021 ataatgcgat cgatagcgat cgatccagac acaataatag gagtaggttt ttccactccg
     1081 atatggtagc aatcctggag ctgcaataca aatataagaa ggttatgcta aagtagagct
     1141 aaacgtatcc tgccatggtt tatatatgac aaaaaatgat ctaaagacaa tcataacccc
     1201 atcattttcg cagattctgc tttgcttgct cataagcata tgtattacga gtattgccta
     1261 ctgggaaact ctcattaata tcttggcgag aaataccctc actagtacta cctccctttc
     1321 cgctagcttt atcggctatt tatcatcatt cggtaaactg ccatttactg gcaatctttt
     1381 tattatcctc ttctggggaa tcctagcgat tttgatttac tgtattggtt tggtgctcgc
     1441 aaatgtgttg atcgatgtgc gcaattccgt ggttgttaaa accagattta ccaagcctgc
     1501 aggaggtcgt aatgtggttg gtcggcttat ggttatggcg atacaagttg tggctgtcgt
     1561 tgtttttatt ggtgtgctgg tagcctgcgg cttgctggtg ttcccgctgt ttattcaaat
     1621 tgcaggcatg gccatattga gcggattaac gctagcgtcc agcgtcttgg ttgttggagc
     1681 aatttgcggt accgctctcg ccttatatgt gctctggacg ctcgcgcagc tcgtatttac
     1741 gctgctttaa tctacccatg caattgccta agcggtgctt tcagacgcgt tggttcgtaa
     1801 ggtacttttt gcattcagac gttagtcact tacctctgaa taacgtataa tgatgcgcat
     1861 gcagccatta gcattatcag ctgaaggaca gcggctgatt cacggtttaa ccgccgccaa
     1921 tacggccgaa cataaacccg actacgacaa ggtacatatt tccggcgttg ggggccaact
     1981 gtacttcgct tatgagcaat tgcggaatgc cgcagagtat ggcgaacggc atttattggt
     2041 ccgaagggca attgagcgct ttttgtaccg caactggcag ctccatgatc gacagccaac
     2101 aaacgaaatg ccacttgaac ttgtttcgga gctaactcat gcccgctacc taaaaaatga
     2161 tagtattccg cgcagttctg tgcggacgat cgaagagatc atccagcgct ataccaagtt
     2221 agcggaggct atccggcagc atcataaggt acctggagat aggttgaagg tctgggtttt
     2281 tcaatcggct tcggttgaaa ttgagcacca attggtttca cacacacaga ccgatgcttt
     2341 tattgacttc gtttatcatc actataagca gcatattgat cccaagatat tcgaccaagt
     2401 taacgaacat acaaccgagg tggtcttgta ttgcgccgtg catcgaacgc tgttcaagtc
     2461 ggatattgct acaaccagat actatgccct ggctgcccgt ctgcatcatg atggcgccga
     2521 cacgctagcc tactttgtta ccttaaacca ggcggttgat cgactgtacc aagcaccgct
     2581 taccaatcgt gtgggacgct tggctaatcg gtcgggcggt ccaatgcgga ttttgcgcga
     2641 agtagtaatg tccaccaacg cgccggagca attacttagc aagcatgatg aattgatgtc
     2701 cagggtggct catgaggccg ggcagcagta taagcttacg cgcaaacggt tgaacgaggg
     2761 tattgttagg agcgtaattt ttttatttat cacaaaggtt ttgcttggag tggcaattga
     2821 ggtaccatac gaccttttgg tggttggcgc ggttgcgtgg acgccgttaa ttatgaatac
     2881 cttattcccg ccactctata tggcaacgat tgggtggggg attcgcactc caggtcgtcg
     2941 gaacacggaa attatccagt cccagctgag ccgcatactt tttcagaccg atgatccgcc
     3001 gatatggtat cgtgtgcgca agcgggttac ctcctcaggt ttaaacaccg cttttaattt
     3061 tgtgtatgcg gttgctttcc tgctttcatt cggcgtgcta atctatctgc tgagcagcct
     3121 tggatttaat attgtgaacg gtgctatttt ctttatcttt ctttcaattg ttagcttttt
     3181 cgggtttcgt cttttggaat cggctcgaga actcgaagtc gtggatcagc gtaaaagctt
     3241 gtttagcgtg gcactcgatt tcttttacac gccattcatc cgcgtgggcc attggcttac
     3301 cgataaatat tcccggctga atgttgttac attcgtaatg gacctggcaa ttgagctgcc
     3361 attaaaaacc agtctccggg ttgtgcgcca gtgggccggt ttcattcgcg ataagcagga
     3421 agagctctaa ggccatctgg ctgagagcgt tttcgaaatt cgcaaagtaa aggtgctgcc
     3481 ggtaccgtac ttggacgtta cagttatgct gccgtggagc agccgcgcaa tcgtcttcac
     3541 tatatatagg cccaacccgg ttccagctac cttgatgctc aaggcgttat tgatgcgcga
     3601 gaatttaacg aatagccggg gcagatcctt atcggcaata ccaacaccgg tatccgtgac
     3661 ggctaagata atgtgccctt ttgattgacc caactgtata tggatggtcc ctttgctagg
     3721 cgtgtacttt gtggcgttgg caataaggtt ctccatggca gtgcgcagac atcgccgatc
     3781 agtaatggtg gtcaatttat caggtatttc taattcaaga tgctgttggt tttctgtaag
     3841 gcgcggttgc aattcggtta agacctcatg aagcaaggct acaacctcaa ccggttcggg
     3901 aagcagcttg agctgctttg attcaagttc agcgacacac agcaggcctt caataatccg
     3961 cagctggctt tcattgctgg tattaagcat ttccagatac ttgcgctgtt tcgcgctaag
     4021 ctttccggta tagttgccaa gcaacatact ggtaaagttt tttatagcgg tcgggtgtgt
     4081 tctcagttgg tgtgaggcca gggcgataaa gacttccttg gagtgttcaa gttcaatatc
     4141 gcgccgcata acctgtaaaa gaaataataa tgccgcaatg agtacaattc cctcgatgcc
     4201 ggcaattatg agaaggtagg ttgaccaagg catagcggaa agttggtaca caagcttaat
     4261 tactaaaaga atgatgctta taccaattcc cgcaatgagt acaccaacac caagactcag
     4321 taaaagctca gcctgctgcc ggcgactaaa ttgggcattc gagaaaagtt tcatatcttt
     4381 agcttaactg agaactgcca aggcttccag atgtagagtt cccgggtaaa aatcaaaacc
     4441 tattaatgag gtgcaccgat aggttggcag caagcgcgca atgtctctgg cttgggtaac
     4501 cggattacaa gaaagataga ttatttgctt gggatgagct tccagtaacc atcgaatcac
     4561 tctaggatgc aagccagccc taggaggatc tacaattacg cagtcgacgt tgtttagcag
     4621 ggtgctgttc atatgctcag ccgctacagc ttcagctgaa taggaggaga tcgcgttagc
     4681 ccgagcgttg cgttcggcga atgctacggc ttcggtatga acttcgatgc cctgtacaga
     4741 tacgccttgc gctgccagcg gcaacccaat tgcgccgaca ccactgtaaa gctcaattat
     4801 tcgacttgaa ggaactatca cttttgttat atattccaag gatttttcaa aaaccggtat
     4861 gtttgattgg aaaaatgcac tatgaggata cgctaggtcg acaccggcta tcgtttcagt
     4921 gacgtagcta tttccagatt gatattcgat ttttccggac gctcccgatc cgggcaacgg
     4981 gcttgcaacc aggaagcttc cgagcttctt ggcggtgata gttttccagc tggcgttaac
     5041 agctgttgtg agtatggtga gcatttggcc tgtgctgggg gcgtaccgga cggtgatagt
     5101 tgccggctca acctgaaagt caccgtcatt aagctgaata agcagattgc gggcggcttg
     5161 attcatggac gagtctccga gtgcacatcc ctcaggtagc gcaatgaatt catcccatga
     5221 tccgcgggca tgaaaagcca gctggagttg gccgtcaatt cgggcaacac taaaatcgag
     5281 gcggtttcgg tatcccagcc gggcaggtga ggggacaaat tcgttgattg gaacggagag
     5341 atgctgctgt gcataagcat ctccgagcat tgctcgtttt agttcgagtt ggtaggtata
     5401 ttcaacgcct tgccaaggcg aacacacaag acaatggtcc tctgctatta ggttccgctt
     5461 agctgagggc tcaataatgt catgcaaaac cgcttcagcg tacctcttgc gctgtttgcg
     5521 aattccaacc cgagccagtt cgccgggtag cggaccaatg gcgaatacgg cacggccgtc
     5581 tgctaagcgg cccatggcct ttccctcagg aacgatcttt tcaaaccgga taagctgact
     5641 catttaggta ttatacctgg tcggtaatgc ttggtgattc tgctactatg aagtgactta
     5701 tggcattttc ttttcatata catcaaacgg acggagcttc ccggcgcggc accattacta
     5761 ctccccacgg cccgatcgaa acacccgcct ttattccggt gggcactcaa gccgcggtga
     5821 aaaccttgga tgcccatgat ctccaacagc tgaaactgcc ggcggttttg gctaacacct
     5881 atcatttata tttacgtccg ggcgttgaga ttgtggagcg gcacggaggg ttgcacgctt
     5941 tcatgcagtg ggacggccca acctttaccg atagcggcgg gtttcaggtc ttttctttgg
     6001 gttttggctt gtctcatggc gtagggaaga ttagtacgat ttttcctgat gaggctgcgg
     6061 ctagtcaggc gaaagctgtt tcaaagccaa aattaatgaa ggtggatgac gatggggtta
     6121 gttttcgctc gcatattgat ggttcccccc accgctttac cgccgaatcc tcaattgggc
     6181 tgcaacagca aattggggcc gatattatcc tggcatttga tgaatgcacc tccccattgc
     6241 atgatgagcg ttataccgtc cgcgcgctcg agcgtacgca tgcatgggca aaacgcagct
     6301 tgtcggcatg gagtaatcgt tcggagcagg cactgtatgg gattgttcaa ggcggcgcct
     6361 acaaaaccct gcggcttgag agcgcagcgt ttattaataa tctggatttt ccaggacatg
     6421 cgattggcgg ctcgcttggt cggagtaagc aggatatgta tgacatattg gagtggacta
     6481 cccctcagct agatgcgcgc aaaccgcggc acttgctggg aattggtgaa atcgaggatt
     6541 tgttcgccgg cgcagcccgc ggtatggata cctttgattg tgttgcgccg actcggatag
     6601 cccgtaacgg ggccgtgtat atttctcctg cagccggagg ctccctagct actaggttcc
     6661 gcttgaatat ttccgccgct caatacgcag atgactcaaa tcccattgat cccacatgcg
     6721 aatgccaggt atgtcaaacc tatagccggg cctacctaag gcacttgttt aacacccatg
     6781 agctgctgtc aatgcgcctt gctaccatcc acaacctgta tttcgtcgga cgtttgatgg
     6841 agcacatacg gaaggcaatt gagcaacgcg agctcacgga gctggccaag caatggggtg
     6901 ttgcccgcct cctgcctcac taagcgtgct agagtagctg tatgagcaga aagcagcatc
     6961 accgcagcta tattttgcag acaatccagc ctggactcgt gggtcttatg gacggttcgg
     7021 tatcaaccct ggcgcccatt tttgccgcag cctttgccac ccaagattcg cacatagcgt
     7081 tcctggtagg gctgtcggct tcaaccggag ctggcatcag tatggcattc gccgaggcct
     7141 tgtcggatga tggtaagata tccggacggg gttctccctg gaggcgtggt gtgatcatgg
     7201 gcgtaatgac gttcctgggc ggaatttttc acacactacc ctttcttcta tcagatattc
     7261 acgttgcgct tatagcagcc tacgctgttg taggggttga gctggttacg atagcgtata
     7321 tccgctatcg gtacttcagc atgagttttt ggtactcctg cctgcaggtg attgttgggg
     7381 gagcattggt ttttgccgct ggtgttttga ttggtgagtc ttaggcgttc aataattcat
     7441 caataaccgc cataatttca atggctcgtt cggtgttaac cggcaaaata gttttgccgt
     7501 aggtttcaac gacgctgtca tcatgccgga caatgaggtg tatgaactgt ttgcgttgct
     7561 tgtcattaat ggtgacttca tgcttccatt caaaatccgg aggggtgtcg gcagtggtcg
     7621 tattaacctc cacccgtccg ccaccttcaa gcggatgtgc ggcgctggta tatacaacgc
     7681 gatcgggtag ctcgtagtac tcaccagtaa cctctataac agggtgtgtg gcggaaaatt
     7741 gctccgcaaa ggtaataaag gatgtagaaa tcattgaagg tccatttttc agcagcatac
     7801 gttagtacgt agtataacaa aacccacacc ttaagcacga gcgctttaga gcttgctaaa
     7861 gccataaata gatggtatag tgcgtggtta gttatgaata tctcccattt tagccaacag
     7921 cacccctacg catatcgccg attattagtt atcgccagta ttgctatgac tctgtctggt
     7981 cttcaatcca tgttaaccgc ggataagccg gtggtgtttg ctgtgggctc tctggcttta
     8041 tcggtcccgc atgctcccgc cggcaatact caagaaaagc ccaaaacggt ttcacaacca
     8101 acatcgttcg gcgggctgac tcgccttcca ggtaaagatg gcagcggcac ccagccagct
     8161 cccgcaatgc tagcctcggc ctcatcgggc gtaggtaatg atatgcgcca gttgggccgg
     8221 atcatgagtg ctgcccgctt tggagatagc cattggcccg cgctcaataa cttatggact
     8281 cgggaatcca attggaatcc cgctgcccgc aatccgagca gtggggcctg cggtatacct
     8341 caggctttgc cgtgttccaa gattccagat atgagtccgg ctggccaact tacctgggga
     8401 cttacctata ttgagcggcg gtacggtaat ccttccaacg cctggcagtt ctggcttagc
     8461 catcattggt attaaagact ggtagtgagc cagcgccgta gcaagctttg tcggctgcgg
     8521 cgataatacc aggcagaaag cactatcccg cttactccaa ttaatccggt gagagctgag
     8581 ccggtttgtg gcagaggtgc cttggtggcg acaggcgtgg gaaccggcac cggcgtcgga
     8641 accggtacgg gagtgggaac cggtac
//
LOCUS       DASVGB010000101         4716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_773742, whole genome shotgun sequence.
ACCESSION   DASVGB010000101 DASVGB010000000
VERSION     DASVGB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773742"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF272_03130"
     CDS             <1..703
                     /locus_tag="VF272_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX7259898.1"
                     /translation="DRLAEIDRERIQTNPLRILDSKEKATQKVIAKAPSILDFLSEAS
                     TSHFQSVREYLDQVDIAYDINPQLVRGLDYYTHTVFEIYGDREGSQSSLGGGGRYDSL
                     VEQLGGASTPAAGFGIGLERIILELDAEGVTIPEPKPVNVYVASLGEPARLAAFGLIE
                     RLLDAGVGAVGAVDKDGIGAQLERANRLDVPLAIIIGQKEVQEETLIVRDMRSGAQEM
                     IPLTSIVKELQDRFA"
     gene            781..1545
                     /locus_tag="VF272_03135"
     CDS             781..1545
                     /locus_tag="VF272_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX7259899.1"
                     /translation="MEWYIIGLIILLLFFSVRIVQQYERGVVFRLGRVQGGVRQPGLR
                     FIIPIIDQMRKVNMRIQTMPVQSQNIITRDNVSIGVSAVAYYLIGDPVKSIVAIDNVI
                     IAINQIAQTTVRNIVGQSALDEVLSDTEKINIAIKKILDAQTENWGVVISVVELKDIQ
                     LPETMQRAMARQAEAEREKRAKVIAAEGEFLSAAKLGDAADIIAAHPIALQLRNLQVL
                     GEIATEKNSTIIFPSQLMNSVQEIKEFVLSEKPTKR"
     gene            1556..1834
                     /locus_tag="VF272_03140"
     CDS             1556..1834
                     /locus_tag="VF272_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259900.1"
                     /translation="MGLYDFLITAFHQFVRIFPESLQWVAALLILIGLIGLLATLVRQ
                     HILFILVVILLLPFLIPVAGEVIADIFAFIRYLVDFIRHIFFGVIIAT"
     gene            complement(1863..2342)
                     /locus_tag="VF272_03145"
     CDS             complement(1863..2342)
                     /locus_tag="VF272_03145"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX7259901.1"
                     /translation="MATALPLNLITTVNAQSTTAPYQIVLDTTQVQAIRSITPAQPDF
                     DTDVLQPLRAKQSAEAARVAAQAARKSAAKSVTAPVGDDAFAQLRYCEAGGIYDRNSG
                     NGYYGAYQYSISTWNNYGGYARADLAPAAVQDEKAHADVARRGFTPWPSCARRLGLL"
     gene            2820..3155
                     /locus_tag="VF272_03150"
     CDS             2820..3155
                     /locus_tag="VF272_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259902.1"
                     /translation="MKGNVALRIVGSSVVFAEDCLNEAEANYGTHFPDPADVAVLVEA
                     MRAELEAQVPLRQVLAMHASDIDDIVHEGQPFKVELIESARAKVACHERRRFAQQARV
                     KARLLTAVA"
     gene            3428..3757
                     /locus_tag="VF272_03155"
     CDS             3428..3757
                     /locus_tag="VF272_03155"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7259903.1"
                     /translation="MQTDCLFCKLSRDGGTVIWHNDDFAAIKDINPKAPLHLLIMPKQ
                     HVENFDQLEVGMAPGLVAAIREVARQEGVSGAYNMKINVGRAAGQEIDHLHVHLLANK
                     EGLSVKP"
     gene            3805..4014
                     /gene="rpsU"
                     /locus_tag="VF272_03160"
     CDS             3805..4014
                     /gene="rpsU"
                     /locus_tag="VF272_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7259904.1"
                     /translation="MLQVTRKDDRESLENMLRRFNKKVQQSGVLGVARRKRQFEKPLT
                     KRAQREIAIRKATRKAVKARQALYR"
     gene            4016..4462
                     /locus_tag="VF272_03165"
     CDS             4016..4462
                     /locus_tag="VF272_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7259905.1"
                     /translation="MALRDTINQNLTASMKEGDALHTGVLRMLKSSLQMEQIKLGHEL
                     TEAEVLKVLQREAKQRKEAIIQYEQGGRDDLVAGENQELEIIEAYLPKQMDDAELEAV
                     VKAVISETGAKDITQLGVVMSAVMQRTEGRADGAKVAQYVRQSLGS"
     gene            4463..>4716
                     /locus_tag="VF272_03170"
     CDS             4463..>4716
                     /locus_tag="VF272_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HEX7259906.1"
                     /translation="MLALAFINQWPPDWQRAVEQVFASLQAQVGRELPSGTIHLKLVN
                     DAEIAALNQQSTGQNYATDVLTFNYQEGADDTYGELADIV"
BASE COUNT         1159 a         1113 c         1287 g         1157 t
ORIGIN      
        1 ggacaggctg gcagaaatag atcgcgaacg catacaaacc aacccgctgc gcatactcga
       61 ttctaaagaa aaggctacgc aaaaggttat tgcgaaagct ccaagcattc tagatttcct
      121 gtcggaagcc agtacctctc actttcaaag tgttcgcgaa tatctcgatc aggttgatat
      181 tgcctatgac ataaatcctc agctagtccg cggtctcgat tactatacgc acacggtatt
      241 tgaaatctat ggtgatcgtg aaggctcgca aagcagtttg ggtggcggcg gtcggtatga
      301 tagcttggtt gaacagctgg gtggcgcatc tacaccggca gctggatttg ggattggctt
      361 ggagcgcatc atcctagagc ttgatgccga aggggtcact attcccgaac cgaagccagt
      421 taacgtgtat gttgctagct tgggcgagcc ggctcgctta gcggccttcg gcttaattga
      481 gcggctgctt gatgccgggg tcggggcggt gggtgctgtt gataaagacg gtattggcgc
      541 ccaactggag cgtgccaacc gcttggatgt gccgctggct attattatcg ggcaaaagga
      601 agtacaggaa gaaaccttga ttgtgcgcga tatgcgctcg ggtgcgcagg aaatgattcc
      661 gctaacatct atcgtgaaag agcttcagga ccggtttgca taggacttca tcagttcctg
      721 gattttgcct tctgcgcttc accggcgcat actagaatga ctaactgagg agttgcgacc
      781 atggaatggt atataattgg tctaattatt cttctgcttt tctttagtgt caggattgta
      841 cagcagtacg aacggggggt agttttccgc ctcggcaggg tacagggcgg cgtgcgccag
      901 ccagggttac ggtttatcat tccgattatc gaccaaatgc gcaaagtgaa tatgcgcatt
      961 caaaccatgc cggtacaatc gcagaatatt attactcgag acaatgtttc aattggcgtt
     1021 tccgcggtag cttactatct gattggtgac ccggtgaagt cgattgtggc gattgataat
     1081 gtaatcatcg ctatcaatca aattgctcaa actaccgttc gaaatattgt cggccaatcg
     1141 gcgcttgatg aggtgctaag cgatactgaa aagattaaca ttgccattaa aaagattctt
     1201 gatgcccaaa ccgaaaattg gggcgttgtg attagtgttg ttgaacttaa ggatattcag
     1261 ctgcccgaaa ccatgcaacg ggcaatggcc aggcaggctg aagccgagcg tgaaaagcgg
     1321 gccaaagtca ttgccgccga aggggagttc ttaagtgccg ccaagctagg cgatgcagca
     1381 gatattattg ccgcccatcc aattgcctta caattgcgaa acctacaagt tttaggtgag
     1441 attgctactg aaaagaactc aacgattatt ttcccaagtc agcttatgaa ttcggttcaa
     1501 gaaataaaag aatttgtcct ttccgagaag ccaacaaagc gctaggggct agggtatggg
     1561 tctgtatgat tttttaatta cggctttcca tcagttcgtc aggattttcc ctgaatccct
     1621 gcagtgggtg gctgcactgc tcattttgat cggcttgatt ggcttgctgg ccactttggt
     1681 tcgccagcat atcttattta tcttggtggt gattttgctt ctgccgtttt tgattccggt
     1741 agccggagaa gtgatcgccg atatttttgc cttcattcgc tatctcgtag acttcatccg
     1801 gcacatcttt ttcggagtaa taattgccac ctaaaaacca cccggaaggg gtggttttta
     1861 ggttacagca agcctaagcg gcgtgcacag gacggccaag gggtaaaacc gcgtcgagcg
     1921 acgtcggcgt gagccttttc atcctggacg gctgccggag ccaggtcggc ccgggcgtaa
     1981 ccgccgtagt tgttccaagt gctaatactg tactggtatg caccgtaata gccgttgccg
     2041 gagttacggt catagatacc accggcttca caatagcgaa gctgggcaaa ggcatcatct
     2101 ccaactggag cggttaccga tttagcagca ctcttccgag cggcttgagc ggcaacccgg
     2161 gcagcttcgg ctgattgttt agcccgcagg ggctgtaata catcagtgtc gaagtcaggc
     2221 tgagccgggg taatggaacg aatagcctgg acttgggtgg tatctaatac tatctggtaa
     2281 ggagcggttg ttgactgagc gttcacggtg gtgatcaggt tcagcggtaa tgctgtggcc
     2341 atttgcaccg gggtgctgag aaggataaaa atccatacat tcagcttgtg taggttatac
     2401 atgggataag aaagaaactc ctttcaccca ttcacgcact ctggtattga gtcgtaagga
     2461 catcactctg cggctttcat tactgaaata cccgtgttta gaagctgccc ttagtatcaa
     2521 aaaatcctgc acataaggca ggaaatttga cacgaacatt actatatcaa atatatcttg
     2581 ttttgtcaag catattattc tacttttgag atgacaattt cagatatgaa tgcgttcgtg
     2641 tggccccaat tagtgccaga ttgggcacaa aataggcaca aatgtggcac aaatccgtgc
     2701 ggcatgggca gatatactta tacattccaa aaatcaggtt taaaatgctt gcataccact
     2761 tatgcttgga gtacggttaa ggtaagccaa tcgtccaacg tacgaaggag ttatttcaca
     2821 tgaagggcaa cgtagcgctc agaattgtcg gttcgtccgt ggtcttcgcg gaggactgcc
     2881 tgaacgaagc agaggcgaac tacggcacgc atttcccgga ccccgccgat gtggcggttc
     2941 tggtcgaggc gatgcgagcc gagctcgaag cgcaggtgcc tctgcgacag gttctcgcga
     3001 tgcatgcctc ggacatcgac gacatcgtcc acgagggcca gccgttcaag gtcgagctga
     3061 tcgagtccgc acgggccaag gtcgcttgtc acgagcggcg ccggttcgcc cagcaagcgc
     3121 gggtgaaagc ccgcctgctg acagccgtcg cctaatcggc atgctgcctc gcctgtttcg
     3181 cccgccacgg gcagcgttcg ggtcatctct cgagtgctca cgcccgccgc ctgaagctct
     3241 gtcgcattga caacttcggg cctgtcccag gcatacggca accgccgcct ccttttcctc
     3301 ggatgtgctt tcgaggtctg ggaggcggta cccgccgcgg catccctctt ttctacttag
     3361 gaggggtgcc accagcgccc ttccatctcg catggcgggg cgttttgctg tctgctacta
     3421 tagtcctatg caaaccgact gtttattctg caaactctcc cgcgatggcg gaacggtaat
     3481 ctggcacaat gatgattttg cagccatcaa ggacatcaat ccaaaagcgc cgctgcattt
     3541 gttgattatg ccgaaacagc acgtggaaaa ttttgaccaa ttagaggtcg gtatggcacc
     3601 cgggttagtg gcggctattc gagaagtagc ccggcaggaa ggagtctctg gtgcttataa
     3661 catgaagatc aatgtcggcc gggcagccgg gcaggagata gaccacttgc atgttcattt
     3721 actcgcaaat aaggaagggt tgtctgtaaa gccataatcg agtacactta cctagttaat
     3781 gctacgaagg gaaggtgatg taacttgctt caagtaacac gcaaggacga tcgagaatcg
     3841 ctcgaaaaca tgctgcggcg cttcaataag aaagtgcagc aatccggtgt cttaggagtg
     3901 gctcggcgga aacgccagtt tgaaaagccg ctcactaagc gcgcccagcg tgagattgcg
     3961 attcgtaaag ccacccgcaa agctgtaaaa gcccgtcagg cgctgtatcg ctaagatggc
     4021 cctccgagat actatcaacc agaacctaac cgcttcgatg aaggagggtg atgctttgca
     4081 taccggtgtg cttcggatgc ttaagagctc tcttcagatg gagcagataa agcttgggca
     4141 tgagttaacc gaggccgagg tgcttaaggt actccaacgc gaagcgaaac aacgcaagga
     4201 agcaattatt caatatgagc aaggtggacg cgatgatctg gtggccggtg agaaccagga
     4261 gctagagatt attgaagcct atcttcctaa gcaaatggat gatgctgagc ttgaagctgt
     4321 ggttaaggct gttatcagcg aaaccggagc caaggacata acccaactgg gtgttgtgat
     4381 gagcgcggta atgcagcgca ctgaaggccg cgctgatgga gccaaggtag ctcagtatgt
     4441 acggcaaagc ctgggtagct aaatgctggc actggctttt atcaatcagt ggcctcccga
     4501 ttggcaacga gcagttgagc aagtatttgc cagcttgcag gcgcaggttg gccgcgagct
     4561 gccaagtggt acgatacatt tgaaattggt aaatgacgcc gagattgccg cgctgaatca
     4621 gcaatcaacc ggacaaaact atgccacaga tgttcttacc tttaactatc aagagggtgc
     4681 cgatgatacc tacggtgaac tggccgatat tgtcat
//
LOCUS       DASVGB010000102         5092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1060245, whole genome shotgun sequence.
ACCESSION   DASVGB010000102 DASVGB010000000
VERSION     DASVGB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060245"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..518)
                     /locus_tag="VF272_03175"
     CDS             complement(72..518)
                     /locus_tag="VF272_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259907.1"
                     /translation="MPEKKSINTITFKNPATPINIAFGIVLVAGLLMYAYFTASTPKA
                     ITEPPVKLAIVTITPTGFIPSTLSIATGTKVVWKNADANPHQLQSNPHPTGDSLPGLK
                     SEILLNAQTYSYAFTKSGSFTYHDYLNPTTNGVIEVHDAKTGGDSR"
     gene            complement(690..2060)
                     /locus_tag="VF272_03180"
     CDS             complement(690..2060)
                     /locus_tag="VF272_03180"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HEX7259908.1"
                     /translation="MDQSENSDTSNTSPSIQPPVPPATAPSPGDDMPPQTPVAKPSPT
                     TSPTSSVMPARLLPDVPSRKPSRKILIVLGVIILLGGGVGVSAAKLFWYDPLHAPTAL
                     FEKMIDAKTGQYSANISYKSQAGSASFLGDKITVSATGSYDVTDEDKPKSANAFKIAL
                     GQFKIAADMITVPKTIYFKLSNSDFLSGFGLQLGTSWYKYPLSEDTSKDKCKQTRTKS
                     GSFMGVTLPKDVPLKDTSRVGLYETIDGHMTSHYKGTVDLDKLVAHLKEVNKELPAEC
                     KIDFGKEDIKNLEVTYEMWRGKDFDRLEAKIVSKGEDKSTTTLTIDTKAYNKSVSIVE
                     PKDAKLFDLFGAESTGTGGEGSSSADAQRKSDLRNVKVALETYYNDNNAYPAAKSYTE
                     LRSHLTTGPTPYMKQLPADPANNGAHIYVYKVAADRQTYTLSATLDNTKDMQAPNGAY
                     TIQSAN"
     gene            complement(2101..3696)
                     /gene="guaA"
                     /locus_tag="VF272_03185"
     CDS             complement(2101..3696)
                     /gene="guaA"
                     /locus_tag="VF272_03185"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7259909.1"
                     /translation="MIQTLTAVTPEVLDEITKEKQLRKTNEVFILFSLGSQFDHLIKQ
                     HLEKQGLYCLVADPAHVTAEDIRKLKPLGIILSGGPVSAHSDPPPLDGHIFDLGIPVL
                     GICLGFQVWARHIGTTVAAATKREFGAHLLTVSNPSELFKGLPDTMPVLESHGDRIEP
                     GDQIEVLASTDHAPVSAGRHEHLWGVQFHPEVTETEFGADIFHNFCFVICGARDVYPA
                     ADIAKHKVEAVQHTVKDGKVLLALSGGSDSSVVTYILKHALQPGQVRGIYIKGIDRPD
                     DEHHVLEYFGSQDWIEVEIIDATDRFLEALKGHEAMADKRVAMRGIYKAVLEHEAKEF
                     GADFIAQGTLYTDISESGGGHQTGAHKAQIKLHHNTGLNFSIPELAPLADSVKDSARS
                     IGRLLGVPEELLIRHPFPGPGLVVRIEGEVTAENLHIARQVDGIYIEELRKWNMYEGV
                     WQAGAVVTRSVTTGSKGDDATSGIIVALWAVWSVNGFTAQWAELSYDFLRHVSQRITN
                     EVPEVGAVVYRISDKPPTTIEWG"
     gene            complement(3858..4334)
                     /gene="rpsG"
                     /locus_tag="VF272_03190"
     CDS             complement(3858..4334)
                     /gene="rpsG"
                     /locus_tag="VF272_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX7259910.1"
                     /translation="MPRKKTKDLSRDITPDIRFNNVLVAKMINKIMHSGKKRLAERLM
                     YSAMDEIEAKLKTEPLEVFETAIKNVSPQVQVRAKRVGGATYQVPMEVRGDRKIHLAM
                     IWILAAAHNKSGRSFDKVLAQEFMDAYNNQGDAIKKRDDTHKMAEANKAFAHFARY"
     gene            complement(4402..4815)
                     /gene="rpsL"
                     /locus_tag="VF272_03195"
     CDS             complement(4402..4815)
                     /gene="rpsL"
                     /locus_tag="VF272_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7259911.1"
                     /translation="MPTINQLIRKPRSAKGRKSKTPALGRTLNSLKGRSYEKSAPLKR
                     GVCTKVSTATPRKPNSALRKVARVRLTNGMEVTAYIPGIGHNLQEHSVVLLQGRGPKD
                     LANVRYTIVRGALDTAGVANRKQGRSKYGAKKASK"
     gene            complement(4843..>5092)
                     /locus_tag="VF272_03200"
     CDS             complement(4843..>5092)
                     /locus_tag="VF272_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259912.1"
                     /translation="TKISLSSDSYLSAASFQDTSRVLIGAAIRGKVDKLRGLKENVII
                     GRLTPVGTGFRKAEADAKQPVAVAAPAGRSLTSPPESD"
BASE COUNT         1214 a         1239 c         1223 g         1416 t
ORIGIN      
        1 aatgacgact aaaaataaag ctgcttttgc tgaaggctgt tgtttttgca tcaaaatcat
       61 aactcgccgc atcaccggga gtctccgccg gttttagcat catgaacttc aataactcca
      121 ttggtggtgg gattaaggta gtcatggtag gtgaagctgc cggattttgt aaaagcatac
      181 gagtaggttt gtgcgtttaa aagaatttcc gacttaagcc cgggtaggct atcacccgtg
      241 ggatgagggt ttgattggag ttgatgcggg ttagcgtcgg catttttcca aacaactttg
      301 gtaccagtgg cgatgcttaa cgtagatgga ataaacccag ttggagttat ggtaactata
      361 gctagtttta cgggaggctc ggttattgcc ttaggggtag aggctgtaaa atacgcatac
      421 atgagcagcc cggctaccaa tactatgccg aaagctatgt ttatgggagt tgctggattt
      481 ttaaatgtaa ttgtattgat gctttttttc tcgggcatgt cacgttgtag atttatagtg
      541 cttatggtta aaggtaatgg tgggattaac gcctgtcaac tgctttcttt ataaaatttc
      601 aataaagttt tattaattga gaccttcggc atgcaaatcc agatatgatt aagcttccag
      661 cgcggaacca attaaactgg tgaagcagct tagtttgcgc tttgaatcgt atacgcgccg
      721 ttgggcgctt gcatatcttt ggtattatca agtgttgcag atagcgtgta ggtctgacga
      781 tcggcagcca ccttatagac atatatatgc gctccattat tagcggggtc tgctggcagc
      841 tgcttcatat agggtgttgg gccggtcgtt aaatggctcc tgagctctgt atagctctta
      901 gcggctggat aggcattatt gtcgttatag tatgtttcaa gggcgacctt aacgttgcgg
      961 agatcactct tacgttgcgc atcggcggaa gaagaacctt caccaccagt gccggtactt
     1021 tcagcaccga agaggtcaaa tagttttgca tctttcggct cgactatgga aaccgacttg
     1081 ttataggctt tggtgtctat agtcagcgtg gttgtgctct tatcctcacc tttgctgaca
     1141 attttagcct cgaggcggtc aaagtccttt ccccgccaca tttcataggt gacttcgaga
     1201 tttttgatat cctccttgcc aaaatcaatc ttgcattctg ccggaagctc tttgttcacc
     1261 tcctttaaat gcgcaacgag cttatcgagg tcaactgtgc ctttatagtg tgaggtcata
     1321 tggccatcga tggtttcata cagaccaacc cggctggtat ccttgagcgg tacatctttg
     1381 ggcagtgtaa cgcccataaa gcttcctgac ttcgtacgcg tctgtttgca tttatcttta
     1441 gaggtatcct cgctaagcgg gtacttatac cacgacgtac caagctgtaa gccgaaaccg
     1501 cttaaaaagt cgctgttgct tagtttgaag taaatggttt ttggaacggt aatcatatca
     1561 gccgcgattt taaactggcc aagagctatt ttaaaggcgt tagcgctttt tggtttatcc
     1621 tcgtcggtaa catcatagct accggttgcg ctaacggtta ttttatcccc cagaaaactg
     1681 gcactgccgg cttggctttt ataggaaatg ttggcgctat actgaccggt tttagcatca
     1741 atcattttct cgaacaatgc cgtcggggca tgcaatgggt cgtaccaaaa cagttttgca
     1801 gcagaaaccc caactccacc ccctagcaaa ataattacac caagaacaat aagaattttc
     1861 cgactcggct tgcgcgacgg cacatctgga agtaaacgag ccggcatcac actgcttgtc
     1921 ggcgatgtag ttggtgaagg ttttgctaca ggagtttgtg gcggcatgtc gtcgccagga
     1981 gatggtgccg tagcaggcgg aactggtggc tgtatcgatg gcgaagtatt tgacgtatcg
     2041 ctgttctcgg attgatccat gcctttaagt aaagcacata ctacttatgc ttagcaaatg
     2101 ctacccccac tcaatggtag ttggcggctt atcagatatt cgatacacta ccgcacctac
     2161 ttccggcacc tcattcgtaa tccgttgcga gacgtggcgt aagaaatcgt acgataattc
     2221 ggcccactgg gcagtgaacc cgttcacgct ccaaaccgcc cataacgcca cgatgatacc
     2281 gctggttgca tcgtctccct tgctaccggt cgttacagac cgcgttacca ccgcacccgc
     2341 ctgccagacg ccttcataca tattccactt gcgtagttct tctatataga taccatcaac
     2401 ctgcctggct atatgtagat tttcggctgt tacttctcct tcaatccgaa ccaccagtcc
     2461 cgggccggga aacggatgac gaattaacag ttcctcgggg acaccgagta gacggccgat
     2521 gctgcgtgcg ctatctttca cgctatcggc caacggagcc agctcaggaa tactaaaatt
     2581 caatcccgta ttatgatgca gtttaatctg agctttatga gcaccggtct ggtggccgcc
     2641 accgctttcg ctaatgtcag tatagagcgt tccttgagca ataaagtccg cgccaaactc
     2701 cttcgcctca tgctctagca ccgccttata aatgccgcgc atagctaccc gtttatcagc
     2761 catagcttca tggcctttca acgcctctaa aaaacgatcg gtagcgtcaa tgatttcaac
     2821 ttcaatccag tcttgagagc caaaatactc cagtacgtgg tgctcatcgt ctgggcgatc
     2881 tatccccttg atataaatgc cccgaacctg gccgggctgc aatgcatgtt taaggatata
     2941 ggtgaccacc gaggaatctg agccgccgga aagagccagt agcaccttgc catctttaac
     3001 ggtatgctgc accgcttcaa ccttgtgctt ggcgatatcg gctgctgggt atacatcgcg
     3061 agcaccgcaa attacaaagc aaaaattatg aaaaatatca gcgccaaact cggtttcggt
     3121 tacctcggga tgaaactgca cgccccataa gtgctcatgc cggcctgccg aaacgggagc
     3181 atgatcggta ctggccaaaa cctctatctg atcgcctggt tcgattctat cgccgtggct
     3241 ttccagtacc ggcatggtat cgggtagccc cttgaacagc tcactgggat tactaaccgt
     3301 aagcagatgt gccccgaact cgcgttttgt tgcagccgct acggttgtgc caatatgtcg
     3361 tgcccacacc tggaatccta aacatatgcc taaaaccggt atgcccaaat caaaaatgtg
     3421 tccatctagg ggaggtggat cgctatgagc agaaaccggg ccacccgaaa ggataatacc
     3481 cagcggtttc agctttctga tgtcttcagc tgtaacgtgc gccggatcag ccactaaaca
     3541 gtagaggccc tgcttttcta aatgctgctt tatgagatgg tcaaactggc ttcccagcga
     3601 aaaaagtata aacacttcgt tcgtcttgcg cagctgcttt tcctttgtta tttcgtcaag
     3661 aacctcggga gtaacagctg tcagggtttg aatcatacct ctagcttatc aagatacgcc
     3721 cggctttcac aaataattgc gcgcacgata caaatacttc ttctataatt gcctactatg
     3781 catgctgata tcattcgatt agatgatatc agacgatatg tgtaggcaag ggaagaacgt
     3841 atttgtgtca gttagtatta atagcgtgcg aagtgagcga aggccttgtt ggcttcagcc
     3901 atcttgtggg tgtcatcccg tttcttgata gcatcacctt ggttgttata agcatccatg
     3961 aattcttgag caaggacttt atcaaacgaa cgcccggact tgttgtgggc agcagctaaa
     4021 atccagatca tcgcgagatg aatcttgcga tcaccgcgaa cttccatggg tacctggtag
     4081 gtagcacctc ctacccgctt ggcccggact tggacttggg gtgagacatt cttaatcgcc
     4141 gtttcaaaca cttccagcgg ttcggtttta agtttcgcct caatctcatc cattgcgcta
     4201 tacatcagtc gttcggctaa ccgctttttg ccgctatgca tgattttgtt aatcatcttt
     4261 gcgaccagta cattattaaa ccggatgtct ggggtgatat cccgactaag gtcttttgtc
     4321 ttcttgcgtg gcatgaatcg tttctccttt gatactaccc gtggccccga aacataccgg
     4381 gcgggtcgtc atgcttaatt gttatttact agctttttta gcgccgtact tactacgtcc
     4441 ctgcttacga ttagcaacac cggcagtgtc taaggcaccg cgaacaatgg tatagcgtac
     4501 attggctaaa tccttcgggc cgcggccttg cagtaagacc acgctgtgtt cctgtaggtt
     4561 gtggccaatg ccaggtatgt aggccgtgac ttccataccg ttggtcaacc gcacacgcgc
     4621 taccttacgc aaagccgagt ttggcttacg tggtgtagcc gtagaaacct tagtacaaac
     4681 ccctcgcttc aatggcgcag acttttcata actgcggcct ttgagcgagt taagcgtgcg
     4741 cccaagagcc ggggtcttcg acttgcggcc tttggcactg cggggcttac gaatgagttg
     4801 gttgattgtg ggcatagatt tcttggttag atgttagttg atttaatcag attcaggggg
     4861 tgaagtcaac gatcggcctg ctggagcagc cacggcaacc ggctgttttg catcagcctc
     4921 ggctttacgg aaaccggtac cgactggtgt taaccgaccg atgatcacgt tttctttcaa
     4981 accgcggagc ttatcaacct tgccgcggat agcagcacca atcagcaccc tagaggtgtc
     5041 ttggaaacta gccgcactca agtaagagtc ggaggacagg gaaatcttgg ta
//
LOCUS       DASVGB010000103         3835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1066794, whole genome shotgun sequence.
ACCESSION   DASVGB010000103 DASVGB010000000
VERSION     DASVGB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066794"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..2464
                     /locus_tag="VF272_03205"
     CDS             278..2464
                     /locus_tag="VF272_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7259913.1"
                     /translation="MTDLLSSLNEPQREAVLTTDGPVLMLAGAGSGKTKALTHRIAYL
                     VAEKKVRPSNILAVTFTNKAAGEMRARVLRLLGRGSDERQYFPYVGTFHAISCRMLRR
                     DYKEAGLTSSFTIFDMADSLSAIKQAMRQIGIDEKRFTPSLIANLISSAKNQLIGPDR
                     YAGYAKGDAQEAAAKVYPGYQRILTEANAVDFDDIIMKTVLLLRNPEVLARWQEQFHY
                     ILIDEYQDTNYAQYQLAKLLAAKHRNLCVVGDDWQAIYSWRGANFQNILDFEKDYPQA
                     KVVKLEQNYRSSKNILDAAHSVISKNQVRSSKKLWTDAAEGAGIKVVTAYDQQQEGDI
                     IAMAIERLMRESHALQRAASSIIPNLQLSDFAVLYRMTAQSRALEEAFLRHNVPYKIV
                     GGTRFYERKEIKDVLAYMRFISNPDDIISFGRIINVPARGLGDRSLERLLDYGRQNGL
                     SLMDTLQSAGLIEVLTPRARTAILNFHTLISGFQEKVDVLPVPELLQQLVTRSGYLTA
                     LDDGSVLAGDRIENVKELLSVAADYEGMNLGEFLEEIALVSDLDNYSTESNAVTLMTL
                     HAAKGLEFPVVFMPGMEEGIFPHNRSLAQASEMEEERRLCYVGMTRAKQELYLLHAQS
                     RLLHGQIQRNVPSRFLGDLPPEVQDEANSLIAAPSTVIPSSWGQVEAVPELAPEKIDL
                     HPGDKIRHAKFGDGIVTHVHGDDLTAAFEGVGTKHLSLSFAPVEKL"
     gene            complement(2475..3164)
                     /locus_tag="VF272_03210"
     CDS             complement(2475..3164)
                     /locus_tag="VF272_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEX7259914.1"
                     /translation="MSKPQVLISNRSLWVMMMLMLLCSAPHAAQAENATGRLTLSPST
                     GSYVVGSTYTIAVTVDSGAADVNAVQADLIYNPALLELVGIDTRSSAFDRKAIETGGD
                     GKVSIVRTKQQTALTGSQLVGSVTFKVLAKSGNAVISFAPTSSIEESTTNRTIWDTNQ
                     FGGRYTMKAIGPVTHSSTNPSLETQPAKSVAEPKLTQKPLSTFDSNLLWYAALMVLIF
                     IFAAIRATKSR"
     gene            3422..>3835
                     /locus_tag="VF272_03215"
     CDS             3422..>3835
                     /locus_tag="VF272_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEX7259915.1"
                     /translation="MQILTWITGIALAVVVIGLVTGMIPPPKNAFADSSKIVSLYYDG
                     QKKVVTTGAPTVAAALTEAGVSIGAGDLVEPNPDTAIPNGFFNINVYRSRPVVVVDGT
                     TRKIIQTALQSNRLIAEQAGLTVYPEDTYSIEVIND"
BASE COUNT          966 a          877 c         1005 g          987 t
ORIGIN      
        1 ctgcatccgg aatctgggca acattagaaa tcactatcat aaaatcggcc gaagcttcga
       61 ttgccagcgt atttagcggg ccaatatgag catcggatag ggtggagagc gacataccaa
      121 gcattatacc gccgattggt atttttttcc ccaatactca aatctgagta ctgttgtact
      181 caattcgtac acacattcga gtatgatttg agtattttga tactcaaata gtagtatcaa
      241 aaaaaattaa gcttcttccc ctgctaaaat agaggcaatg actgatttat tgagcagttt
      301 aaatgaaccg cagcgcgaag ctgtgctaac aactgatggt ccggttttaa tgctagctgg
      361 agccggcagc ggtaaaacca aagcgctgac ccatcggatt gcctacttgg ttgccgagaa
      421 aaaggttcgg ccctccaaca ttttagcggt tacttttacc aataaggctg ccggggaaat
      481 gcgcgcccgc gtgctgcggc tgcttggacg cggcagtgat gagcgccagt attttccata
      541 tgtgggaaca tttcatgcta tttcctgccg gatgttgcgc cgcgactata aagaagccgg
      601 acttacatcc agtttcacta tttttgacat ggccgatagc ctgagcgcta ttaagcaggc
      661 aatgcgtcaa atcggcattg atgaaaagcg ctttacaccc agcttgatag caaatttgat
      721 atcaagcgca aaaaatcagt tgattggccc agatcgctat gccgggtatg ccaagggaga
      781 cgctcaggaa gcagccgcta aagtctaccc gggttaccag agaattctga ccgaagccaa
      841 tgcggtggat tttgatgaca ttattatgaa aacagtgctt ctcttacgta atcccgaggt
      901 gctagcgcgt tggcaggagc agttccatta tattttgatt gatgagtatc aggacactaa
      961 ctatgcccaa taccaattgg caaaattgct cgcagccaaa catcgcaacc tctgcgtggt
     1021 tggcgatgac tggcaggcaa tctattcgtg gcggggggct aactttcaaa atattctcga
     1081 ttttgagaag gattatcctc aggccaaggt agtaaagctg gagcagaatt atcgttcaag
     1141 caaaaatatt ctggacgctg ctcacagtgt tatcagtaag aaccaggttc gttcaagcaa
     1201 aaagctctgg accgatgccg ctgaaggtgc tggcattaag gtagtaacgg cgtacgatca
     1261 acagcaggaa ggcgacatca tcgctatggc gatagagcgg ctaatgcgtg aaagccatgc
     1321 actgcaacgg gctgccagta gcatcattcc caacttacag ctcagtgatt ttgcagtcct
     1381 ctaccgtatg acggctcaat cgcgcgctct ggaagaggct tttttgcgcc ataacgtacc
     1441 gtacaaaatt gttggcggca cgcgttttta tgagcgtaaa gaaattaaag atgtgttggc
     1501 ttatatgcga ttcattagca atccggatga cattattagt tttggccgta ttattaacgt
     1561 gccggctcgt ggtctcggcg accgcagttt ggaacgctta ttggactatg gccggcagaa
     1621 cgggttatcg ctgatggaca cgctccagtc ggccggttta attgaagtgc taactccgag
     1681 ggctcgcacc gctatactca atttccatac gctcatttcg ggcttccagg aaaaggttga
     1741 tgtattaccg gtgccggagt tgcttcagca actcgtaact cgaagcggtt atttgactgc
     1801 cttggatgat ggcagcgtct tagccggcga ccgcattgaa aacgtgaagg agctcctaag
     1861 cgttgcggct gactatgaag gcatgaatct gggtgaattt ttggaggaga tcgctttagt
     1921 tagcgacctt gataattatt cgaccgaatc aaatgccgta actttaatga cgttgcatgc
     1981 cgcgaagggc ctggagtttc cggtggtctt tatgccgggg atggaggaag gaatttttcc
     2041 gcataaccgc agcttggctc aagcttccga aatggaggag gagcgacgct tgtgttacgt
     2101 aggcatgaca cgggccaagc aagagctgta cttgctgcat gcccagtcgc ggctactgca
     2161 cggccaaatc cagcgcaatg tgccctcccg ctttttgggc gatttaccgc cggaagtaca
     2221 ggatgaagct aattcattga tcgcagcacc gtctaccgtg attccttcca gctggggcca
     2281 ggttgaagcg gtgcctgagc tagcgccgga gaaaattgat ttgcatcccg gcgataaaat
     2341 tcggcatgca aagtttggcg acggaattgt aactcatgtt cacggagacg acctaaccgc
     2401 tgcgtttgaa ggcgtgggca ccaagcacct aagcttgtct tttgcgccgg ttgaaaagct
     2461 ttagcggcga gttgtcagcg cgattttgtg gctcgaatgg cggcgaaaat gaatatcagc
     2521 accatcaatg cggcatacca tagcaggttt gagtcgaagg tggacagtgg tttttgagta
     2581 agcttaggtt ctgcaaccga cttcgcgggc tgggtttcta atgatgggtt ggtggagcta
     2641 tgtgtaaccg ggccaatcgc cttcatggtg tagcgcccgc caaattgatt ggtgtcccaa
     2701 atggtacggt tggtagtcga ctcttcgatt gaagaggtgg gagcaaagct aataacagcg
     2761 ttgccggatt tagccagtac tttaaaggtt accgatccaa ccagctggct gcccgttagc
     2821 gcggtttgct gttttgttcg gacaatacta actttgccat caccacccgt ctcaatggct
     2881 ttcctatcga aagcgcttga tcgggtatcg ataccaacaa gttcaagcag ggcagggttg
     2941 tagatgaggt cagcttggac ggcattcacg tcggcagcac cgctatccac agtaacggca
     3001 atggtgtaag tactgccaac aacatagctg ccggtggagg gagacagggt aaggcggccg
     3061 gttgcatttt cagcctgtgc ggcatgcggc gccgagcata acagcataag catcatcatg
     3121 acccagagtg atcgatttga aattaaaact tgaggcttac tcataggtac cttacgttag
     3181 ggatcattaa gtgcgcggtt agtgtaacat gaccataagc gctttagcaa tcgggagaga
     3241 tactgcttat gcttacactt gacttattgc cgccatccca gtaagcttaa gcataagctc
     3301 tcctatctgg tggaggctca aaaacaaaga cgccaaaggt cgctttcttt ctttaacttt
     3361 agaggcaact attttaggca tcatccattc agcactgcag gctccggccg acggtaggtt
     3421 tatgcagatt cttacatgga tcaccggcat cgcattagcg gtggtggtga tagggcttgt
     3481 aacggggatg atcccgccgc ccaaaaacgc ttttgctgat tcatccaaaa tcgttagtct
     3541 ttattatgat ggacaaaaga aggttgttac taccggtgct cccacggttg cggctgctct
     3601 taccgaagca ggtgttagca ttggagccgg tgatttagtt gagcccaatc cggataccgc
     3661 aatccctaat ggctttttca atattaacgt ttatcgatca cgtccggtgg ttgtggttga
     3721 cggcactact cgtaaaatta ttcaaaccgc tcttcaaagc aaccgcctga ttgccgagca
     3781 ggctggttta acggtttacc ctgaggacac ctatagtatc gaggtgatta acgat
//
LOCUS       DASVGB010000104         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_973988, whole genome shotgun sequence.
ACCESSION   DASVGB010000104 DASVGB010000000
VERSION     DASVGB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973988"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF272_03220"
     CDS             complement(<1..376)
                     /locus_tag="VF272_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259916.1"
                     /translation="MPIENELHPREENPEAPKASLDVRIMEARGALNDRSANSEIHPR
                     PLRAAWLEGQSENVEAAVARALSHVEDETPVAKVVQHREADLSLTEDATFVNAYLVAF
                     EAKATINGEAVPVGVMNFESVVS"
     gene            534..3395
                     /gene="uvrA"
                     /locus_tag="VF272_03225"
     CDS             534..3395
                     /gene="uvrA"
                     /locus_tag="VF272_03225"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7259917.1"
                     /translation="MQDKIVVRGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGLMEKPDVDQIDGLSPAISIDQKSSSRNPRSTVGTV
                     TEIYDYMRLLWARIGVPHCPLCGREVLRQTSGQIVDQILTLPAEARVMVLAPVVDDKK
                     GEHRGVAAELKKAGFSRLRVDGTLYDVDEFPELDKQRKHTVEAVVDRLVVDEESRGRI
                     TDSVEQALTLGEGIVNVLEAESGKTHTFSEHYACPVHREISLPEVAPRNFSFNSPHGA
                     CPHCTGLGTRLEVDPELVIPNRRLSIAEGAIRPWSRTTARVTWYTRMLGAVAKQYGFD
                     ITIPVSQMKPADLKVILYGAGGEGVSLRGPGGRTYHTAYEGVVPNLERRYHETDSDFV
                     RREISKYMAEHECPVCQGKRLKPEILAVTVSGKSIVDVTNRSIEQAGVFFDSLTLNDR
                     DAHIAYQILKEIRARLSFLRDVGLSYLTLDRSANTLAGGEAQRIRLATQIGSSLMGVL
                     YILDEPSIGLHQRDNDRLIGTLERLRNLGNTVIVVEHDEETMLAADWLIDIGPRAGEH
                     GGEVIAAGTADEVAANPESLTGQYLSGVREIAIPAKRRKGNGKIITVKNAREHNLKNV
                     DVSIPLGRLVIVTGVSGSGKSSLVNDILAKKLSQVFHRSSDVPGRHDDIIGLENLDKV
                     ISVDQSPIGRTPRSNPATYTGVFGDIRELFAATPEAKIRGYKAGRFSFNVKGGRCEAC
                     KGDGIIKIEMHFLPDVYVTCEECKGKRYNREALEIHYKGKTISDVLDMTVESAAEFFE
                     NIPPIRRKIETLNSVGLGYIRLGQPATTLSGGEAQRIKLATELARRSTGRTLYILDEP
                     TTGLHFEDVKKLLEVLNALVESGNTVLVIEHNLDVVKSADWIIDMGPEGGGGGGQVIA
                     EGTPEQVAQAKDSHTGFYIKRLLGKQKTLKKESKKKVTA"
     gene            3405..>3739
                     /locus_tag="VF272_03230"
     CDS             3405..>3739
                     /locus_tag="VF272_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7259918.1"
                     /translation="MVSSEKLTAKLKTLPKQPGVYFHKDAKGKIIYIGKAAVLKNRVG
                     SYFQQSRHRDFKTKILVGEIVDVDWITVESEVEALFLESEFIKRYMPKYNIDLRDDKH
                     FIYIKIPMK"
BASE COUNT          963 a          818 c         1015 g          943 t
ORIGIN      
        1 gtgaaaccac tgactcgaaa ttcatgacac caacgggtac ggcctcgcca tttatggtag
       61 ctttggcctc aaaggctact aggtaggcgt taacaaacgt tgcgtcctct gtgagagaca
      121 aatcagcctc tcgatgctga actacttttg caaccggcgt ctcgtcctca acatgactta
      181 aggcacgggc gaccgctgct tcaacgttct cactttgacc ttcaagccat gcagcacgaa
      241 gcgggcgcgg atgaatctcg ctgtttgctg agcggtcatt aagcgcgcca cgggcttcca
      301 tgatacggac atcaaggctg gccttaggcg cttcgggatt ttcttctcga ggatgtaatt
      361 cgttttcgat aggcatctga ggatattgct ccagcttaca tgaaattttt gttataaatt
      421 agattacatt gtaccaaacc ctactcaagc gaccacagtt gccctaagtc ttgataaaca
      481 tgcttaaaat atgtataatc tgaacctgta tttgcgggga aaaaggtttg ggtatgcaag
      541 ataagattgt ggttcgaggt gcacgagagc acaacctaaa aaatattgat gttgaaatac
      601 cgcgcgacaa actggtggtt attaccggct tgagcggatc gggaaagtct tcattagcgt
      661 ttgatactat ttatgccgaa ggccagcgcc gctacgtgga atcgttaagc gcctacgccc
      721 ggcagttctt gggacttatg gagaagccgg acgtcgatca aattgatggt ttaagcccgg
      781 caatttcaat tgatcaaaaa tccagctccc gcaatccgcg ttcaacggtg ggaacggtta
      841 ctgaaattta tgattatatg cgtttgttgt gggcgcggat tggcgttccg cactgtccct
      901 tatgcgggcg tgaagttctg cgtcaaacct ccggccagat tgtagatcaa attctaacgc
      961 tgccggcaga agcacgggtg atggttttag cccccgtcgt tgacgacaaa aagggtgagc
     1021 atcggggtgt ggctgccgag ttaaaaaaag ctggtttttc gcgcttgcgg gtggatggta
     1081 cgctttacga tgtagatgag tttcctgagc ttgacaagca gcgcaagcat accgttgaag
     1141 cggtggtcga tcgcctggtg gttgatgagg agtcgcgcgg ccgaattacc gattcggtgg
     1201 agcaagccct taccctggga gagggaattg ttaatgtgct ggaagccgag tcgggcaaga
     1261 cccatacctt tagcgagcat tacgcctgtc cggtgcatcg tgaaattagc ttgcccgaag
     1321 tcgccccacg caatttttca tttaactctc cgcatggtgc ctgcccgcac tgtaccggtc
     1381 ttggcacccg gctggaagtg gatccggagc tggtaattcc caaccggcgc ttatcaattg
     1441 ccgaaggggc gattcgcccg tggtcgcgca ctactgctcg agtgacctgg tacacccgca
     1501 tgttgggagc cgtagcgaaa cagtatggtt ttgatattac tattccggtg agccaaatga
     1561 agccggctga cttgaaagtt attttatatg gagccggggg cgaaggtgtg agtttgcgcg
     1621 gtcctggcgg ccggacctac cacaccgctt atgagggtgt ggttccgaat ctggagcgtc
     1681 ggtatcatga aacggactcc gacttcgttc ggcgcgagat tagtaagtac atggccgaac
     1741 atgagtgtcc ggtgtgccaa gggaaacggt tgaaacccga gattctagcg gtaacggtct
     1801 ccgggaaatc gattgttgac gttactaatc gttcaattga gcaggcaggg gtgttttttg
     1861 actcgctaac gcttaacgac cgtgacgctc atattgccta tcaaattcta aaagaaattc
     1921 gggcacgctt gtcattcctt cgagatgttg gcttgagcta tttaacactt gaccgcagcg
     1981 cgaacacatt ggcaggaggg gaggcgcagc gaatccggtt ggctactcaa attggctcca
     2041 gtttaatggg agtactctat attctggatg agccttcaat cgggttgcat caacgcgata
     2101 atgaccgtct gattggtaca ttggaacggc tgcgcaatct tggcaatacg gtaattgtag
     2161 ttgagcacga tgaggaaacc atgctagccg ccgattggtt aatcgatatt ggaccgcgag
     2221 ctggagagca tggtggcgaa gtaattgctg ccggcacggc tgacgaggtt gccgcgaatc
     2281 ctgaaagcct cactggccag tatctttcgg gagtgcgaga aattgctatt ccggcgaaac
     2341 ggcgcaaggg caatggcaaa attattactg ttaaaaatgc ccgcgagcat aatttaaaaa
     2401 atgttgatgt tagcattccg ctgggccgtc ttgtaatagt taccggtgtt tcgggttctg
     2461 gaaaatcgag tttggtaaat gatatcctcg ctaaaaaact ttcccaagtc tttcatcggt
     2521 caagtgatgt gcctggcagg catgacgata ttataggact ggaaaacctg gacaaagtta
     2581 tttcggttga tcaatcgcca atcggtcgta ccccgcgatc gaatccggct acgtacactg
     2641 gtgtgtttgg cgacatccgg gaattgtttg cggctacacc ggaagctaaa atccgaggct
     2701 ataaagccgg ccggttcagc ttcaatgtaa aaggtggccg ttgtgaggcc tgtaagggcg
     2761 atggcattat taaaattgag atgcacttct tgcctgatgt ttacgttacg tgtgaagagt
     2821 gtaagggcaa acgctacaac cgggaagcct tggaaattca ctataaaggc aaaaccattt
     2881 ccgacgtgct cgatatgacg gttgaaagcg ccgctgaatt ttttgaaaat attccgccaa
     2941 tccgacgtaa aattgaaacc cttaacagtg tcggcttggg atacattcgc ctggggcaac
     3001 cagctacaac attaagtggc ggtgaagccc agcggattaa acttgcaacc gagctggccc
     3061 ggcggagtac cggccgaaca ttgtatattt tggatgaacc aacaaccggc ttgcattttg
     3121 aagatgttaa aaaattactt gaggtgctga atgcactggt agaaagcggg aataccgtat
     3181 tggtaattga gcataaccta gacgtggtga agagcgccga ctggattatc gacatggggc
     3241 cggaaggcgg tggcggtggc ggtcaggtta ttgctgaagg aacaccggaa caggtggcgc
     3301 aggccaagga ttcgcatact ggattttata ttaagcggct actaggcaaa caaaaaacac
     3361 tcaaaaagga atcgaaaaag aaagtaaccg cgtaaaatag acctatggtt tcttccgaaa
     3421 aacttacagc caagttaaaa actttgccga agcagccggg cgtctatttc cataaagacg
     3481 ccaagggcaa gattatatat attggcaagg cggcggttct aaaaaaccgg gttggttcat
     3541 attttcagca aagccgtcat cgtgatttta aaaccaagat attggtaggc gagattgttg
     3601 acgttgactg gattacggtc gagtccgaag ttgaggcact gtttcttgag agcgagttta
     3661 ttaagcgcta catgccgaaa tataacatcg atttacgcga tgataagcat tttatttata
     3721 ttaaaattcc gatgaaaga
//
LOCUS       DASVGB010000105         6074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_525050, whole genome shotgun sequence.
ACCESSION   DASVGB010000105 DASVGB010000000
VERSION     DASVGB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525050"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VF272_03235"
     CDS             complement(<1..363)
                     /locus_tag="VF272_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259919.1"
                     /translation="MSSSFLSIIRIIAVVVILLSIYGLVTASGLFGIGGDFFSSSEQK
                     AAEGNTGNVDAIGGILSAGLGMAWLIVSLITFCLSLIVYFVATRKLKKQKQLLETKVD
                     SVSPPRESQPETRVGEESG"
     gene            complement(423..497)
                     /locus_tag="VF272_03240"
     tRNA            complement(423..497)
                     /locus_tag="VF272_03240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Gln,seq:ctg)
     gene            complement(557..1156)
                     /locus_tag="VF272_03245"
     CDS             complement(557..1156)
                     /locus_tag="VF272_03245"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7259920.1"
                     /translation="MFTLPNLPYEPDALEPHIDSITMEIHHGKHHQTYVDKLSAALDG
                     HDDFLAMDVYELMSHLEEVPTEIRKAVRNNGGGHANHSFFWETMSPNGGGEPSGDLAS
                     KIDSTFGSFSDFQNQLKQAGLDRFGSGWAWLVKTSSGLEITSTPNQDNPWMEGDTPLL
                     GVDVWEHAYYLKYQNKRPDYIDAWWNVVNWEIVEDRYQT"
     gene            complement(1180..1851)
                     /gene="trmD"
                     /locus_tag="VF272_03250"
     CDS             complement(1180..1851)
                     /gene="trmD"
                     /locus_tag="VF272_03250"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7259921.1"
                     /translation="MKIDIITLFPDMFKGPFDMSMMWKAQERGLASIDFHDLREFGLG
                     PRRTVDDTPYGGGDGMLLKPEPVVAAIEHAKEGNKNAKVIILSPQGKRFDQAYAAELT
                     TEDGLILVAGHYEGFDERIIAYADIELSIGDYVLTGGELPVMTVVDAVVRLIPGVLGG
                     EQSTHVESFSDGLLEYPQYTRPVEFRGQKVPDVLQNGHHAEIEAWRAKEAAAKTKRNR
                     PDLLK"
     gene            complement(1874..2311)
                     /locus_tag="VF272_03255"
     CDS             complement(1874..2311)
                     /locus_tag="VF272_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX7259922.1"
                     /translation="MADLREDFVQYVVKALVNNPEAVTLGRNQDEKGEYLELRVDPSD
                     MGKVIGKAGATAKSIRTLLRGVGAKDDLPPLPLKIVDPERSEHDTRDHAPVAAAATAA
                     GATESAAETSVEEAAPTPEEKDEHQALHERTKREMQELADFEL"
     gene            complement(2369..2908)
                     /gene="rpsP"
                     /locus_tag="VF272_03260"
     CDS             complement(2369..2908)
                     /gene="rpsP"
                     /locus_tag="VF272_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7259923.1"
                     /translation="MQVIRLARGGRSKYPTYRIVAAESARATTGKFIEILGHYNPHTK
                     ELTIKKEETLKRIGFGAQPSNSVIKLLQREKMELPDWVQFKTKTPRKIEKAEEPASEV
                     KTETPVKAEGVIEEAAPKEPAMEAESSEEPAEEEVRVAEAATENAAEVEDKATAAGKT
                     ETAEDTEAKAEAASKTEQS"
     gene            complement(3055..3222)
                     /locus_tag="VF272_03265"
     CDS             complement(3055..3222)
                     /locus_tag="VF272_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259924.1"
                     /translation="MIERNLPGLMALLAPAVRLVALAVHSSPAPAPLAQAASIQFYST
                     SEPSHPQAAER"
     gene            complement(3371..4075)
                     /gene="rnc"
                     /locus_tag="VF272_03270"
     CDS             complement(3371..4075)
                     /gene="rnc"
                     /locus_tag="VF272_03270"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7259925.1"
                     /translation="MEATKKTFDDLQAQLGVQFKNPELLERAFIHRSYLNEHPKSGLE
                     HNERLEFLGDAVLELAVTDYLYSTYPNPEGDLTNWRSALVKTESLSSVAEQLELDQYL
                     KLSRGEAKGNARSRALIHANSVEALIGAIYMDQGYSAAQKFILTNIISRLEQILETGS
                     WMDPKSRFQEIAQEHEGFTPHYKVLEEMGPDHDKEFVVGVFVGDRLCGKGKGSSKQAA
                     QQVAAAVALKSYRAQA"
     gene            complement(4075..4566)
                     /locus_tag="VF272_03275"
     CDS             complement(4075..4566)
                     /locus_tag="VF272_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7259926.1"
                     /translation="MSTNQKKNHKHLKQKRPPRLKAIREYTAGGMVFRRRGAGKIDIL
                     MIQDRKGRWTIPKGHVEEGESFEETATREISEETGLKHLVIRDKLDKIFFFYRREGSL
                     IYMTTHIFLIEALSDTDALVPEDSEGIVDVKWFNTEEALKTIEYKDTEQLFKLGLEKL
                     KVS"
     gene            complement(4529..4984)
                     /gene="nusB"
                     /locus_tag="VF272_03280"
     CDS             complement(4529..4984)
                     /gene="nusB"
                     /locus_tag="VF272_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7259927.1"
                     /translation="MASNRHLGRIVAMQTLYECDFRESAGVEVDKAATLERNLGEFSD
                     AIDDKQFVKDLVKGVRANEQEIDGIIGPAAPEWPVDQIAKIDKIILRMGVYELLLQKE
                     VPPKVVINEAVELAKSFGGENSSKFINGVLGTIYRESDVYQPEEEPQAS"
     gene            complement(4991..5161)
                     /gene="rpmF"
                     /locus_tag="VF272_03285"
     CDS             complement(4991..5161)
                     /gene="rpmF"
                     /locus_tag="VF272_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7259928.1"
                     /translation="MAVPKKRTTKSARGQRRSHDGLAVTQLQAASNGSLLPRRLHKAV
                     SLGLTKYIKRAQ"
     gene            complement(5148..5570)
                     /locus_tag="VF272_03290"
     CDS             complement(5148..5570)
                     /locus_tag="VF272_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7259929.1"
                     /translation="MQIDVSDILRQGEGALEEFVIDEETPELDGLVLASPISGTIRII
                     GTNTGVVAGGQLVAAVTLECSRCLKEYAQELTFSFEAEFEDYPDEDQFPIDKQGRIDL
                     ADPVRQELEVHLPLKALCQEDCTGIKLNQTKDSEYGRT"
     gene            5811..>6074
                     /locus_tag="VF272_03295"
     CDS             5811..>6074
                     /locus_tag="VF272_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259930.1"
                     /translation="MRVQIKLEDETKGAAENAARQMGYPSLAALLKVLASNLARQNMI
                     GKPEIEPNKDILPMPPQNTPPPPSLEPTIVQRPTLPAPEQFTIW"
BASE COUNT         1439 a         1615 c         1393 g         1627 t
ORIGIN      
        1 cccggactcc tcgcccacac gagtttcagg ttgcgattct ctaggagggc taaccgagtc
       61 aacttttgtt tcaagtaatt gcttttgttt ctttaatttt ctagtagcta caaaatacac
      121 tatcaaggac aagcagaaag taatcaagga cacaattaac cacgccattc caaggccagc
      181 gctaagtata ccgcctatcg catcaacatt tcccgtatta ccttcagcag ccttttgctc
      241 tgaacttgaa aagaaatcgc caccaatgcc aaataacccc gaagccgtca ctagaccata
      301 aatagaaagt agaataacaa ccacagcaat tattcttatt attgagagga aggatgaact
      361 catggtttcc ttgtatctaa gttgagtaag tagattttgt catagaataa gcataagtgc
      421 aatggctggg gcggtaggat tcgaacctac gatctcttga gccagaatca agcgccttac
      481 cacttggcca cgccccattg atgccatttt gaaccctggc atagtagcaa tttataagct
      541 gttgatcaag gatggcctag gtctgatagc ggtcttccac gatctcccaa ttcactacgt
      601 tccaccaggc atcaatgtaa tccggccgct tgttttgata ctttagataa taggcatgct
      661 cccagacatc cacgccgagc aggggcgtgt ccccttccat ccatgggttg tcttggtttg
      721 gtgtcgaggt aatttccagt ccgctgcttg ttttcaccag ccaggcccaa ccgctgccaa
      781 accgatcaag acccgcttgc ttcaactggt tttgaaagtc ggaaaagctg ccgaaggtac
      841 tgtcgatttt agacgcaagg tcgccgctgg gttcaccgcc gccgtttgga gacatagtct
      901 cccagaaaaa gctgtggtta gcgtggccgc cgccattgtt acgtaccgcc ttccgaattt
      961 ccgtaggcac ctcttccaaa tggctcatca gttcatagac gtccatggcc agaaagtcgt
     1021 catggccatc aagtgccgcg ctcagtttat cgacgtaggt ttgatgatgc ttgccatggt
     1081 gaatttccat ggttatggaa tcaatgtgag gctccagagc gtcgggctca tagggtaaat
     1141 tgggtaacgt aaacatgcag tacctcctta ggttctcttt tattttaaca gatcgggacg
     1201 gttgcgtttg gtttttgcgg cagcctcttt ggcgcgccaa gcttcaattt cagcatgatg
     1261 gccgttttga agtacatccg gaaccttttg gccgcggaac tcaaccggac gggtatattg
     1321 aggatactcc aataggccgt cgctgaatga ctccacatgc gtgctttgct ccccgccaag
     1381 cacgcccgga atcagccgaa cgaccgcatc aaccactgtc atgactggta actccccgcc
     1441 ggttaacacg taatcgccaa tgctcagctc gatatcggca tacgcaatga tgcgctcatc
     1501 gaagccttcg tagtggccag ctactaagat aagaccgtcc tccgtggtta gctcggcagc
     1561 gtaagcttga tcaaaacgct taccctgagg tgaaagaata ataactttgg cattcttatt
     1621 cccttcctta gcatgctcaa tggccgccac taccggttca ggcttcagca gcatgccgtc
     1681 tcccccgccg tacggcgtat catcaactgt tcgtcgcggt cctaagccaa actcgcgtag
     1741 gtcatggaaa tctatgctag ctaagccgcg ctcttgagcc ttccacatca tgctcatgtc
     1801 gaatggcccc ttaaacatat cgggaaatag cgtaataata tcgattttca ttgcaattag
     1861 gataaggcca gagctatagc tcgaagtcgg ctagttcctg catttcacgc ttagtacgtt
     1921 catgcagcgc ttgatgttca tctttttctt ccggtgtcgg ggccgcttct tcaacggaag
     1981 tctcggctgc tgactctgtc gcacccgcag cggtagcggc ggcagctacg ggagcgtggt
     2041 ccctagtgtc atgctcgctg cgttccggat caacaatttt cagcggtagc ggcggtaaat
     2101 catctttggc accgacaccc cgtagcaagg tacgaatgct tttagcagta gcaccggctt
     2161 tcccaataac cttacccatg tcggatggat caactcgaag ttccagatac tcgccttttt
     2221 catcctgatt acggcccaac gtgactgctt cgggattgtt aaccagcgcc ttgactacat
     2281 actgtacgaa atcttcgcgt aaatcggcca caggaatgcc tcactcattt aaacgtagct
     2341 ttataatagc gcaggttgcg cgaggaaact aggactgctc ggtcttagag gcggcttcag
     2401 cctttgcttc ggtgtcttcg gcagtttcgg ttttacctgc cgcagtagcc ttatcttcga
     2461 cttcggcagc gttttcggtg gctgcctcgg ctacgcgcac ttcttcttcg gcaggctctt
     2521 cggaactctc tgcctccatg gctggctctt taggtgcggc ttcctcgatt accccttcag
     2581 ccttcaccgg tgtctcggtc ttaacctctg aagctggctc ttccgccttt tctatcttgc
     2641 gcggtgtttt ggttttaaac tgaacccaat cgggcaattc cattttttcg cgctgtaaaa
     2701 gcttaattac gctattagaa ggctgggcgc caaagccgat ccgcttcagc gtttcttctt
     2761 ttttaatagt taattccttg gtatggggat tatagtggcc taaaatttca ataaacttcc
     2821 cggttgtagc tcgcgctgat tcagccgcaa caatacggta ggttggatat ttagagcggc
     2881 cgccacgggc caagcgaata acttgcatag gagagattgt accgattcta tctggaaaag
     2941 tcaatcatta cggggctaaa acgcaagcag ccgcccgaga atgaaacatc ccgaacggca
     3001 gctgacgaat ggcatggggg tagtacggtc tagcttcgcg gaggagatgg ggactcagcg
     3061 ctcagcagcc tgcggatgcg atggctcgga ggtgctgtag aactgaattg aggcggcctg
     3121 ggcgagcggg gccggagccg gcgaagaatg gacggcaagt gccaccaaac gaacggccgg
     3181 ggcaagcagc gccatcaatc ccgggagatt gcgttcaatc atgcggagca aaggattcct
     3241 tccgtgggag tgtgaatgag gcgagcgtcc tgactcggga atgaaaccgg tgtgtaccta
     3301 caccatgtga gggagcgacg ccaaacgtcc tcttcgtgat accacgaaac cggctatggt
     3361 tacaagaata ttatgcttga gccctgtagc ttttcagggc aacggcagcg gctacttgct
     3421 gggctgcctg tttgcttgag cctttgccct tgccacacag gcggtcgccc acgaagacac
     3481 cgaccacaaa ctccttgtcg tggtccggcc ccatttcttc cagcactttg taatgagggg
     3541 taaagccctc atgctcctgg gcgatctcct ggaaccgact cttaggatcc atccagctcc
     3601 cggtttctaa aatttgctcc aaacggctaa taatattcgt aagaatgaat ttttgtgccg
     3661 cactatagcc ttgatccata taaatagcgc cgattaaagc ttccaccgaa ttggcatgaa
     3721 ttagggcccg gctgcgagca ttgcccttcg cttcccctcg gctgagcttc agatattgat
     3781 caagctcaag ttgttcagcc accgaactta agctttctgt tttcaccaag gcactccgcc
     3841 agttagttaa atccccctcc ggattgggat aggtactgta aagatagtcc gtaaccgcta
     3901 actccagtac ggcatcacct aaaaactcga gacgctcatt gtgctcgagc ccagacttag
     3961 gatgctcatt taagtatgag cggtgaataa atgcccgctc taataattcc ggatttttaa
     4021 actgaacgcc caactgcgcc tgcagatcat caaaggtttt cttggttgct tccattaact
     4081 tactttaagc ttttctagcc caagcttaaa gagttgctcg gtatctttat attcgatagt
     4141 cttaagcgct tcttcggtat taaaccattt tacatcgaca atcccctcac tgtcttcggg
     4201 tactagcgcg tcggtatcgc tcaaggcctc aattaaaaaa atatgagtgg tcatgtaaat
     4261 taagctgcct tcacggcgat aaaagaaaaa gattttatcc agcttgtccc ggattaccaa
     4321 gtgtttcaag ccggtctcct cgctgatttc gcgcgtagcg gtttcctcaa agctttcgcc
     4381 ttcttcgaca tgccctttag gaatagtcca ccgacccttg cgatcctgaa tcatgaggat
     4441 gtcaatttta cccgcacctc tccggcgaaa taccatgccg ccggcggtgt attcgcgaat
     4501 cgcttttagc cgaggcggcc gcttttgctt aagatgcttg tggttcttct tctggttggt
     4561 agacatcact ttcacgataa atcgtgccga gcactccgtt aataaacttg cttgaatttt
     4621 cgccgccgaa cgatttggcc aactccactg cttcgttgat tacgactttc ggcggtactt
     4681 ctttctgcaa tagcagttca tacacgccca tacgaagaat aattttgtcg attttggcta
     4741 tttgatcaac cggccactca ggtgctgccg gaccaataat gccatcaatc tcttgttcgt
     4801 ttgcacgaac cccttttacc agatctttaa cgaactgctt atcgtcaatc gcatcggaaa
     4861 attctccgag attgcgctca agcgtcgccg ctttatcaac ctcgacccct gcactttcgc
     4921 gaaaatcgca ttcgtacaag gtttgcattg cgacgatacg tcccaaatgg cggttcgagg
     4981 ccacgctacg ttactgagcc cgcttaatat acttggtcag ccccaagctt actgccttat
     5041 gcagacgccg cgggagcaaa cttccattgc tggctgcttg taactgcgta accgctaagc
     5101 catcgtggct gcggcgttgg ccgcgggctg atttggtagt ccgtttttta ggtacggcca
     5161 tattcactat ccttcgtttg gtttaatttt atcccagtac agtcttcttg gcaaagcgct
     5221 ttcagtggta agtgcacctc aagttcctgt ctcaccggat cggccaagtc gattcgtccc
     5281 tgcttatcaa tggggaattg gtcctcatcg ggatagtctt caaactcggc ttcgaacgaa
     5341 aacgtaagct cctgtgcata ctcttttaaa caccggctac actccagagt aacagctgcc
     5401 accagctgcc cgccggccac taccccggta ttagtgccaa taatccggat cgtaccgctt
     5461 atcggtgatg ccagaacaag gccatcgagc tcaggagttt cttcgtcaat gacgaattcc
     5521 tccaacgctc cttcgccttg tcttaatatg tcacttacgt ctatttgcat aggcttcaca
     5581 gggggttagt atacccgtaa aaaccgaatt tggcaagcct ataccggatc gcccttttga
     5641 tactttatta tctcttttat tctcgtactc acaaaggagt acaaatatac tccttttatg
     5701 cacacatttg agtacctttt aagtataaaa tgacgctgct ttgagtacac aaataaaata
     5761 aaattttact gcttaacgtg gatctaatat ttcctttgta ggatagaaac atgagagttc
     5821 aaatcaagtt agaagatgaa acgaaaggtg ccgctgagaa tgcagcgcgc caaatgggat
     5881 acccgtctct cgcggcgctt ttgaaagtat tggccagtaa cttagcacgc caaaacatga
     5941 taggtaaacc tgaaattgaa ccaaataaag acatccttcc gatgccaccg cagaataccc
     6001 cgccaccccc tagtcttgag cctacgatag tccagcggcc cacattgccc gcaccagagc
     6061 aatttacaat ctgg
//
LOCUS       DASVGB010000106         5339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_514639, whole genome shotgun sequence.
ACCESSION   DASVGB010000106 DASVGB010000000
VERSION     DASVGB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514639"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF272_03300"
     CDS             <1..181
                     /locus_tag="VF272_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259931.1"
                     /translation="PVVAVAASPGRGGAVPQPVAGGVGGGVLPATGSGLDWIIATLLA
                     LASGFWYRWRQANTA"
     gene            335..1096
                     /locus_tag="VF272_03305"
     CDS             335..1096
                     /locus_tag="VF272_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7259932.1"
                     /translation="MLLAGVMVGRLWSGYIEVSLLVVAAIGLLLFKLRFGLIVLLIFS
                     FGLWRGISTDFSRTPLGDKLGQTVSLVGVVADDPSVTAGNQVGFKLGNVEMDGRGIGQ
                     EVQVYTPYKSLQRGYRVAVMGKLKPRRGSIPVQASFARVAIVSDQVSLLERVRQRFFI
                     SARVAMPEPASGFGLGLLIGVKSLIAKDLQEVLTTVGLSHLIAVSGYNLTIIIHAMRR
                     LSDYLSHFSVTALSLWLIAGFLVVLVLVRQLLEPV"
     gene            1114..1719
                     /locus_tag="VF272_03310"
     CDS             1114..1719
                     /locus_tag="VF272_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7259933.1"
                     /translation="MIAYYGYAVRPLVIIAIPAAITVAWKPDYLIRDISWQLSFLAFL
                     GVLVLAPLMVERWVRRPNTIKLLVIESTAAQVTTAPLILGLFANLSLISPLSNVVILP
                     LVPLAMLLSFATGVTAMASPLIGAWVALPATGLLGLMVGLSQWFATWPYANVNFQVST
                     SGVLVLYALVVVLTFSLYRWRARHPEAVSAQEYSPMDVKLG"
     gene            1816..2184
                     /locus_tag="VF272_03315"
     CDS             1816..2184
                     /locus_tag="VF272_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259934.1"
                     /translation="MTSIPAGITMGRYPPGIPRPGQEREYERLRLLVGTYQSIEVRRG
                     HISPKVLLMGEPDGGVHRPSKVHRYDVKKGTPVWLKITSVTTDSNGWPMVTGSRNDTE
                     FGFRPDYIYPLPILSLKIGS"
     gene            2265..3011
                     /locus_tag="VF272_03320"
     CDS             2265..3011
                     /locus_tag="VF272_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7259935.1"
                     /translation="MSGHSKWHSIKHQKGIADARRGAVFTKLANAIALATKKGGADPE
                     MNFSLRLAIQKAKAANMPAANIERSIKRGSGQLGGDQIEEITYEGYGPGGAALIIETA
                     TDNRNRTGPEVRTALSKSGGRLADAGSVVYQFDQRGVIRFEPDDLEAATLDAIEAGAD
                     DVEAGDKELVVYTVANQLNAVQTKLTEMGYAVKSSELAYIAKNTVVIDDEKVAGQLLR
                     LMDALEDMDDVTTTHANFDFSDALAASLSK"
     gene            3008..3490
                     /gene="ruvC"
                     /locus_tag="VF272_03325"
     CDS             3008..3490
                     /gene="ruvC"
                     /locus_tag="VF272_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7259936.1"
                     /translation="MKILGIDPGTATTGFGVIEKRGSTLTFVDAGVILTPPEQPMPER
                     LSEIYAGLNEILAEHQPDCVAVELLYFASNVTTAITVGQARGVVLLAASEHHVAVAEY
                     TPLQVKQAITGYGRANKKQIQEMVKMMLGLSGIPKPDDAADGLAIAITHANQMVGAAS
                     "
     gene            3487..4062
                     /gene="ruvA"
                     /locus_tag="VF272_03330"
     CDS             3487..4062
                     /gene="ruvA"
                     /locus_tag="VF272_03330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7259937.1"
                     /translation="MIARLRGIVIEKTIGSAIIEAGGVGYEVVVTAADHGSLTMGEEA
                     SLHIFEQIREDAHNLYGFSNLASKHFFEQLLSISGIGPKVAINILSAAGLPQLQSAIA
                     GGDPEILRGVAGVGKKTAERIVVELKTKMEAVGLTAGGMGEPVSSGDAAYQALIGLGY
                     TPAQAAEALRGVPSSIKKEQDRVKAALKQLI"
     gene            4110..5138
                     /gene="ruvB"
                     /locus_tag="VF272_03335"
     CDS             4110..5138
                     /gene="ruvB"
                     /locus_tag="VF272_03335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7259938.1"
                     /translation="MIERVTVAAEQTEDPVEQQLEETLRPKSFDAYVGQQLLKDTLQL
                     SIQAAAARNEAVDHILLYGPPGLGKTTMAGVIASEMRSNLRITSGPAIERAGDLASIL
                     TNLVDGDILFIDEIHRLPKTVEEVLYPAMEDFGIDIMLGKGPSAKSMRLDLPKFTIIG
                     ATTRVGALGAPFRDRFGMVHRLEYYTLEEMVLILKRSARILGISLDDEAAQEIAMRSR
                     LTPRIGNRLLKRVRDFAQVKYDGAVKAEVARKALEMLEIDELGLDTADRKLLAAIIDN
                     YGGGPVGLQTLAALCSEEQTTIEDVYEPYLMQIGMLDRTSRGRRVTPKAYLHLGVEPP
                     AAAQGSLM"
     gene            5135..>5339
                     /locus_tag="VF272_03340"
     CDS             5135..>5339
                     /locus_tag="VF272_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259939.1"
                     /translation="MKTAPGPRNAKKIYTQQHIGLDRDVTRSRVLHREIQELLASVKK
                     DFASRAEWECWCRRTSRVGAAARA"
BASE COUNT         1248 a         1228 c         1535 g         1328 t
ORIGIN      
        1 acctgttgtg gcagtggctg catcaccggg tagaggtgga gccgttccac aacccgttgc
       61 cggaggagtt gggggagggg tcctccctgc taccggatct ggccttgatt ggataattgc
      121 cactctctta gcactagcat caggtttctg gtatcgctgg cgccaggcca acacagccta
      181 aaataaaaac ctacgttagc taatagaaaa gacgggcaca tgctcgtctt ttctatggat
      241 tatatttaat caacgtttaa tcctgttggc gtaaactgag ccaatgaggt catggcgcag
      301 ggggagaacc gctcaaatat gctggatttc ggggatgctg cttgctgggg tcatggttgg
      361 tcgtctatgg agcggttaca tcgaggtaag tcttctagtg gttgcggcaa tcgggctgct
      421 gcttttcaaa cttcgctttg gtctcattgt cttactaatt tttagttttg gcttatggcg
      481 cggaattagc acagatttct cacgcactcc tcttggtgat aagctcggcc aaactgtcag
      541 tttagttggc gtggtggcag atgatcccag cgtaacggct ggcaatcaag tgggcttcaa
      601 acttggtaat gtagaaatgg atggacgcgg tatcggccaa gaggtacagg tttatacgcc
      661 gtataagtcg ctgcagcgag ggtatcgggt agctgtgatg ggcaaactaa agccgcgaag
      721 gggatcaatt ccggtgcagg ccagctttgc tcgggttgcg atagtgtctg atcaagtatc
      781 gttattggaa cgcgtacggc aacgcttttt tatctctgct cgagtggcta tgcctgagcc
      841 ggcatctggg tttggattgg gtttgttgat tggcgttaaa tcgctgattg ctaaagattt
      901 acaagaggta ttaacgacgg tagggttatc gcacttaatt gccgtgtccg gctataacct
      961 tacgattatt attcatgcca tgcgccgatt gtctgactac ttatcgcatt ttagcgtaac
     1021 ggcgcttagc ctatggttga tagctggctt tctcgtggta ctggttttag tgcgccaatt
     1081 gttagagccg gtttagtatc aggtctaggg ctgatgattg cctattacgg gtacgccgtt
     1141 cgcccgttgg tgattattgc aattccagcc gccataaccg tagcctggaa gccggattat
     1201 ctgatccgcg acattagctg gcagctgtcg ttcctggcat ttttaggggt gctggtacta
     1261 gcgccgctta tggtggagcg ttgggtgcgc cgcccgaata cgattaagtt gctggtaatt
     1321 gagtcaacgg cggctcaagt gacgactgcg ccgttaattc taggcttgtt tgcaaatctt
     1381 tcactgattt cgcccttatc caatgtagtg atattgccgc tggtcccatt ggctatgctg
     1441 ctcagctttg cgaccggcgt tacggctatg gcgtcgccgc tgattggcgc ctgggttgcc
     1501 ctgccggcta ccggtttgct cggtctcatg gttggactca gccagtggtt tgctacatgg
     1561 ccgtatgcca atgttaattt tcaagtttca acgtccggcg tcctagtgct ttacgcgttg
     1621 gtagtggtgc ttacgtttag cttgtatcgc tggcgagcac ggcatccaga ggctgtatca
     1681 gcgcaagaat attcgccgat ggatgttaaa ttgggatgac aaacataacc attgtggtta
     1741 cgtttaaatc agcatactat tcatgtaagt tgaggaacgg attcaactag catcatcgca
     1801 tcgcaaggag gagcagtgac cagtattcct gctggaatca cgatgggccg ctacccccct
     1861 gggatcccgc gtccgggaca ggaaagggaa tacgagcggc ttcggctgct tgttggtacg
     1921 tatcagtcaa ttgaagtgag gaggggccac atcagtccga aggtgctgtt gatgggggaa
     1981 ccggacggcg gagtccatcg gccatcaaag gttcatcgct acgacgtcaa aaaggggacg
     2041 ccggtgtggc tgaagatcac gtcggtcacc accgacagca acggatggcc gatggtcacg
     2101 ggaagccgaa atgacaccga gttcgggttt cggcctgatt acatctatcc gcttcccatc
     2161 ctcagtctca agatcggctc gtgatgcgcg agggccgggc ttcattgccc ggccctcatg
     2221 ggctttcaag cattccaaca gatgagtggt ataataaata tactatgtcc ggacattcta
     2281 aatggcactc aatcaaacat caaaagggca ttgctgatgc tcggcgcggc gctgtgttta
     2341 cgaaattagc taatgctata gctctggcaa ccaaaaaagg cggggctgat cccgaaatga
     2401 atttttcgtt gcgtttggcg attcaaaagg ctaaggcagc caatatgcca gcggcaaaca
     2461 ttgaacgttc cattaagcgc ggatcggggc agctcggcgg cgaccaaatt gaagaaatta
     2521 cctatgaagg ctatggtccc ggcggggctg ctttgattat tgaaacggct accgacaatc
     2581 gtaatcggac cggtccggaa gttcgtacgg cgctgagcaa aagcggcggc cggttagctg
     2641 acgccggatc ggtagtgtat cagtttgatc agcgcggggt tattcgcttt gagccggatg
     2701 atttggaggc agcaacctta gacgccattg aagctggggc cgatgacgtg gaagctgggg
     2761 ataaggagct ggtggtgtac actgtagcaa accaattaaa tgccgttcaa accaagttga
     2821 ccgaaatggg ctatgccgtt aaatcctcgg aacttgccta cattgccaag aacaccgtag
     2881 taattgatga cgaaaaggtt gccggtcaat tactgcgctt gatggacgcg cttgaagata
     2941 tggatgacgt tacgactacg catgccaatt ttgatttctc ggatgcgctt gccgcaagct
     3001 taagcaaatg aagattctgg gcatcgatcc cggcacggcg acgaccgggt ttggtgttat
     3061 tgaaaagcgg ggaagcacat taacctttgt tgacgccggg gttattctga cgccgccgga
     3121 gcagccgatg cctgagcgcc tcagcgaaat atatgccggg ctcaatgaga tccttgctga
     3181 acatcagccc gattgcgtgg ctgttgaatt actgtatttt gcctcaaacg taaccacggc
     3241 tataacggtc gggcaagcca ggggagtcgt cttattggcg gcgtctgagc atcacgtggc
     3301 ggtagctgaa tatacgccat tgcaagtaaa gcaggcgatt accggttacg gacgggccaa
     3361 caaaaaacag attcaggaaa tggtaaaaat gatgcttggt ctcagtggta tccccaaacc
     3421 ggatgacgct gccgacggtt tggcaattgc gattacgcac gctaatcaga tggttggagc
     3481 agcttcatga ttgcccgact gcgcggtatt gtcatagaaa agaccattgg cagtgccatt
     3541 attgaagctg gcggggtagg atacgaagtg gtggtaacgg cagccgatca tggttcgctg
     3601 accatggggg aggaggctag cctgcatatt tttgaacaga ttcgcgagga cgcccataat
     3661 ttatacggct ttagcaatct cgctagcaag catttctttg aacaactttt aagcataagc
     3721 ggaatagggc cgaaagtagc gattaatatt ctttcagctg cgggtttgcc ccagttgcag
     3781 tcggcgattg ccggtggcga cccggaaatt ttgcgcggcg tggccggcgt cggtaaaaag
     3841 accgccgagc ggatcgtggt ggagcttaaa acgaaaatgg aagcggttgg gctgacggca
     3901 ggaggcatgg gtgaaccggt ctcatctggc gatgccgcct accaagcatt gatcggattg
     3961 gggtatacgc cagctcaagc cgctgaagct ttgcgcgggg tgccatcatc tattaaaaaa
     4021 gaacaagatc gggtaaaagc ggcactgaaa caattgatct agtttcggat tttgttcgct
     4081 tattttattt cacttgatat aatacagcta tgattgaacg cgttacggta gcggccgaac
     4141 aaaccgaaga cccggtcgag cagcagttag aagagacact acggccgaaa agctttgacg
     4201 cctatgttgg ccagcaattg ctgaaagaca ccctacagct gtcaatacag gcagccgctg
     4261 ctcgcaacga ggcggtagac catattttat tgtatggacc gcctggattg ggtaaaacca
     4321 ccatggccgg cgtgattgcc tcggagatgc gcagcaacct gcggattacg tcaggtccgg
     4381 cgatcgaacg tgcaggagac ttagcctcca tcctaactaa cttggtagat ggtgacattt
     4441 tgtttattga tgaaattcat cgcttgccca aaacagtgga agaggtgcta tatccggcaa
     4501 tggaagattt tggcattgat attatgcttg gtaaaggccc gagcgccaaa agcatgcgct
     4561 tggatttgcc caagttcacc attatcggtg ccaccactag agtgggcgca ctgggcgcac
     4621 catttcgcga tcgttttggt atggtgcacc gccttgaata ttacacgctt gaagaaatgg
     4681 tattaatttt gaaacgctcg gctcggatac tggggattag cttagacgat gaagctgccc
     4741 aagaaattgc tatgcgctct cgtttgactc cgcggattgg caaccgctta ctcaagcgcg
     4801 tacgggattt tgcccaagta aagtacgatg gagcggtaaa agccgaggtt gcgcgtaaag
     4861 cgctggagat gctggaaatt gatgagctgg gtctcgacac cgccgatcga aagttactgg
     4921 ctgccattat cgacaactac ggcggcggtc cggttggcct gcaaaccttg gctgccctgt
     4981 gctcggaaga gcaaaccacg attgaagatg tctatgagcc gtacctgatg caaattggca
     5041 tgctggatcg gacatctcga ggacgccggg ttacgcctaa ggcctacctg cacttaggag
     5101 ttgagccgcc ggcagccgcc caaggatcat tgatgtgaag accgctccgg gtccgcgcaa
     5161 tgcaaaaaag atatatacac agcagcatat cggccttgat cgtgatgtta ctcgatcccg
     5221 ggtgctgcac cgcgagattc aagaattact ggctagcgtg aaaaaggatt ttgcttcccg
     5281 ggccgaatgg gaatgctggt gtcgccggac gagtcgagtt ggcgcagctg ctcgagcgt
//
LOCUS       DASVGB010000107        10992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_923985, whole genome shotgun sequence.
ACCESSION   DASVGB010000107 DASVGB010000000
VERSION     DASVGB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 10992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10992
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923985"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VF272_03345"
     CDS             complement(<1..1053)
                     /locus_tag="VF272_03345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7259940.1"
                     /translation="MIKPLGRIILRYRYQLGVVGVLLLALFMRLHQLPSLPPGLHGDE
                     AANGLDILRMFEQHDFRPFYNTNGGREALFFYLQAIGVQIFGTTIFGLRIIPALLGVA
                     AVALTYIWLSSWYGKRVAVTAALLMAVSPWAVTISRDGYRAGLMAVMVPLTLWLYTKA
                     VQTRQLAWFITAGAALGLGFYTYPTFWFIPLLLAALLAYTALRNRVLVRELLPDIGKS
                     LAAAAAMLIPLVWYGVQHAGALFGRPTGVSVFNNPDGLAGSVGALGTSVAKTLLMFNV
                     HGDENYRHNLGGQPELNIFVGAMFILGILVCVVRRKKLRYAGLLAVLFIMLLPAMLTA
                     EGLPHALRSYGAMPAAM"
     gene            complement(1050..1517)
                     /locus_tag="VF272_03350"
     CDS             complement(1050..1517)
                     /locus_tag="VF272_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7259941.1"
                     /translation="MIPFSHGDLSQVGKFGIVGVLNTIIDFTIFNILTSKRFHMHKVT
                     ANIISTTTAMIFSFFANRQAVFESGTGNPVVQAVLFFAVTAFGLYIIQTGVLYLLAVR
                     WSWPDRLIRHLLKLTRLNRLISHSFALNNGAKLAGTLCSLSWSFYMYKHVVFR"
     gene            complement(1559..2938)
                     /gene="dnaB"
                     /locus_tag="VF272_03355"
     CDS             complement(1559..2938)
                     /gene="dnaB"
                     /locus_tag="VF272_03355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7259942.1"
                     /translation="MSSVATPKTTQKPLTILPHNLEAEAGLLGGILVDKEALVKVADI
                     VAADDFYVDRNGIVYRAMIDLYEARQPIDLLTLSNRLESVKELEKIGGSAYLTELVEQ
                     VPTAAHVAHYGRIVAHKATLRRLISAASTIAGLGHDESTPLDGLLDKAEQTLFGVSQK
                     HLKQNFIPISSVLAESFDRLDELHKDKNSLRGVPTGFKSLDGVLAGLQKSDLIILAAR
                     PSMGKTTLAMNIAHHVAVKEGIPVGIFSLEMGKEQLIDRLLASESGIDAWKLRNGRLD
                     DSDFPKINTAMAVLSEAPVYIDDSASTNVMEMRTKARRLQSEHDLGLIIVDYLQLISG
                     RNAGGSDNRVQEVSEISRGLKGLARELNVPVLALSQLSRSVESRTPPIPMLSDLRESG
                     SIEQDADVVMFIYREDYYRGKEAEQPGSAQILVRKHRNGPTGEIELYFHPEQLLFRDV
                     DRLHAGHNM"
     gene            complement(2931..4403)
                     /gene="dnaX"
                     /locus_tag="VF272_03360"
     CDS             complement(2931..4403)
                     /gene="dnaX"
                     /locus_tag="VF272_03360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7259943.1"
                     /translation="MGELALYRKYRSASFSTVIGQSHVIETLQQAISSDRLSHAYLFT
                     GPRGVGKTSVARLLARAANCTSEDPGKRPCGTCSNCKIEIGSHLDLIEIDAASNRGID
                     DARALREKISSAPAMGRYKVYIIDEVHMLTTEAFNALLKTLEEPPAHAIFILATTEAH
                     KLPVTIISRTQQFRFKLITQDDLLQHLGAIAKQEGITIEAEALELLATTSRGGFRDAI
                     SLLDQIASSGTSPLTVAVTRQLLGHSDPELLEAIDAALMVQDTVAALQAVDQAIAQGV
                     QAGQLTNQLVMFERHKLQALVHDGAPDSELQRTAQVIQELLPVLKSSWPEISLEVALV
                     ALTTPDTASSHPVAAKVRPGTAVSAKPAVPSDTPTAVAALQAEPLDSVPDPDLWVKAL
                     TQIKQRNNSLYALLRSCSLEFDNDRVTIGCKFSFFRDRLKEPKNLEIIEQTLSRVYGR
                     KLRVMPQLEAATATVPATADSSAELMSSALEILGGEVIHE"
     gene            complement(4476..4985)
                     /locus_tag="VF272_03365"
     CDS             complement(4476..4985)
                     /locus_tag="VF272_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259944.1"
                     /translation="MAELADKQLKTLKKLIETAETNLAGAKELLASLVGDEEAGTVLT
                     SIPVGSKVIEGHFDGQNMVGPDGKLYPVPANYASKSKLVEQDGLKLTIGEDGSFLYKQ
                     IAPAQRHKLIGTLNLKDGQYYVEAQGRDYRVLFASVTYYKAQPGDQITIVIPEEGEAE
                     WAAIEAVLA"
     gene            complement(5082..5501)
                     /gene="rplL"
                     /locus_tag="VF272_03370"
     CDS             complement(5082..5501)
                     /gene="rplL"
                     /locus_tag="VF272_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7259945.1"
                     /translation="MAEETASKEETKEIPKKFKELVGQIDKLSVLELSELVKVLEDHY
                     GVSAAAPMAMAAPAGGGGETEAAEEKSAYTVILAAAGEQKINVIKAVRELTGQGLAES
                     KAIVDGAPKPIKENVPKAEAEEAKAALEAAGATVELQ"
     gene            complement(5516..6046)
                     /gene="rplJ"
                     /locus_tag="VF272_03375"
     CDS             complement(5516..6046)
                     /gene="rplJ"
                     /locus_tag="VF272_03375"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7259946.1"
                     /translation="MAITRQEKEQAVSQLQNELAELKLVVLTDYRGLSVSEISELRDK
                     LRSEGMSYRVTKNTLLRIAAKNTPALKGVDPSTFTGPMALAISTNDEVAPARVIFQYA
                     KEHQALEIVGAVTGDGELLSVAQVKALATLPTREQLLGQLVGTIAAPLSSFVGVMGAN
                     VRGIVTVLNAIKESKE"
     misc_feature    complement(6053..6182)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(6337..6576)
                     /locus_tag="VF272_03380"
     CDS             complement(6337..6576)
                     /locus_tag="VF272_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259947.1"
                     /translation="MAKLRMDGTRGLLMQVVQRCMNDLGIVETWKHCDITTHLIRTDY
                     RFASGNFRFFGGRVLLLYQSETLRGFGFFFIMYML"
     gene            complement(6700..7965)
                     /locus_tag="VF272_03385"
     CDS             complement(6700..7965)
                     /locus_tag="VF272_03385"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7259948.1"
                     /translation="MNRFIELTHDEYARQRDTLNLIASENYPSPKALGLMGSVWSTKY
                     AEGSPGKRYYAGVTNADEMELLVQQKALEVYDSTGEYGVNVQVLSGSPANTMVYLAML
                     EPGDKILSLSLANGGHLSHLHATSNYKKFFEHGTYDVKEVTPGTFEVDSDDYMQKLKS
                     FKPKLVIIGFSAYPRKYEFEAMCKAAHEAGALVLADIAHINGLVAAGLHDSPFKAGAA
                     GADFVSMTTHKTMRGPRGAMLFAKNDYIGDINKTIFPGTSGGPHLQQMVAIGQSLLEI
                     LGEDKYPDGIDFKTYSQTVLDTSKALEDGCKEGGLGVVSPTENHLCLIKLPDEVDSLD
                     FQKRLEAVGIITNRNMLPFDTKSAWRPSGMRFGTAALASRGLDVDGAHELGTLIGDMA
                     TGKADDTTAAQSVKAKLAGLTWYYDDIKI"
     gene            8019..8936
                     /gene="thyA"
                     /locus_tag="VF272_03390"
     CDS             8019..8936
                     /gene="thyA"
                     /locus_tag="VF272_03390"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX7259949.1"
                     /translation="MRQYLDLLQDIQKNGTIKGDRTGTGTTSVFGRQMRFNLSEGFPA
                     VTTKKLYLRSIIHELLWFLQGSTNIKYLVDNDVHIWDEWPYRAYLQKQEKPVPPSDSE
                     EWQIGIKEFTEKIKADDQFAKEHGELGPVYGYQWRSWPTPSGEHVDQIQKAIDMIKTN
                     PNSRRIIVSAWNVAEIDEMAKAGLPPCHTLFQFYVADGKLSCQLYQRSADTFLGVPFN
                     IASYALLTMMIAQVTGLKPGDFVHTFGDTHIYSNHTNQVKEQLSREPRPLPTMKVNPN
                     VTSIFDFTFDDFELVNYDPHPAIKAPIAV"
     gene            8938..9426
                     /locus_tag="VF272_03395"
     CDS             8938..9426
                     /locus_tag="VF272_03395"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX7259950.1"
                     /translation="MGINLIVAVAENGVIGRNDGSLPWRLPADLARFRQITLHHPIIM
                     GRTTYESIGRILPDRRNIIITRNSSFHIDGAEVVHSLESAIALASAGDKEIFIIGGGS
                     IYEQALPRADVLYLTEVHAEPEGQVLFEYNRDEWEEVERDDRPADDLNQYPYCFVKLV
                     RK"
     gene            complement(9435..10124)
                     /locus_tag="VF272_03400"
     CDS             complement(9435..10124)
                     /locus_tag="VF272_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEX7259951.1"
                     /translation="MTKPGLFIVIEGTDGAGKGTQTELLHKRLSKEGHKIAVFDFPQY
                     GKPSAHFVERYLNGKYGSVDEVNVYQASLYYAMDRLEAGFAIREALADGKIVLANRYV
                     GSNMAHQGARFADSEERKAFFEWIEDLEFGLLGIPRPDLNVVLHMPAALAQKLVDKKK
                     QREYLGTKKRDIHEDDLHHLQKAEQAYLEMAKLFPTIFHLIECAEGGKVATKKEVSEK
                     VWSAIKAKLPR"
     gene            complement(10124..10795)
                     /gene="dcd"
                     /locus_tag="VF272_03405"
     CDS             complement(10124..10795)
                     /gene="dcd"
                     /locus_tag="VF272_03405"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX7259952.1"
                     /translation="MGVFSDREIKAALKSGQIVIDPFEPANVKGSSVDVRLGEWFYVT
                     DSATNQDYYNPFDAYSVEDYFKGPLKAISNKDWAEHHNRRPWKNIPLNHPIIVLRPHE
                     RILAHTVEFAGIAYEGTTTMQARSTWGRNGIVVCKDAGWGDPGYINRWTMEIQNDNDV
                     SVPLPVGMRVAQLVFYHMAKADKTYGESDKYQVKGSIQEVKKAWKPEMMLPRAYTDTI
                     SMPRV"
     gene            complement(10833..>10992)
                     /locus_tag="VF272_03410"
     CDS             complement(10833..>10992)
                     /locus_tag="VF272_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7259953.1"
                     /translation="MSNATTAIDAVMKAKPAAAKGTYVQSAALSSTMGPGIKLDVQAA
                     IATANPKR"
BASE COUNT         2740 a         2928 c         2572 g         2752 t
ORIGIN      
        1 cattgccgcc ggcattgctc cgtaggaccg cagggcatga ggcaagcctt cagcggtcag
       61 catagccgga agtagcatta tgaagagcac ggcgagcaac ccggcatacc gcagcttttt
      121 gcgccgcacc acacagacta aaataccaag tataaacatt gcaccgacaa aaatatttaa
      181 ttccggttga ccccccaggt tgtgtcggta gttttcatcg ccgtgaacat taaacattag
      241 tagagttttc gccacacttg tacctaaagc gccaacgctg ccggctaatc catcagggtt
      301 gttaaagacg ctgacaccgg tggggcgtcc aaagagtgct ccggcatgtt gaacgccgta
      361 ccacacgagt gggataagca tggcagcggc tgccgctaaa cttttgccga tgtctggcag
      421 taattcgcgg accagcaccc ggttgcgcag tgcggtatat gccagcaatg cagccaggag
      481 cagcgggatg aaccagaagg tcggataggt gtaaaatcct aaacctagcg ccgcaccggc
      541 cgtaataaac caggctaact gacgggtttg taccgccttg gtatacagcc ataaggtcaa
      601 tggcaccatc accgccataa gcccggctcg gtacccatcg cgcgaaatcg ttaccgccca
      661 tgggctaacc gccataagca aggctgctgt aaccgcgact cgctttccgt accaactgct
      721 caaccaaata taggttaaag caaccgccgc cactcctagc aatgctggaa taattcgtaa
      781 accgaagata gtagtgccaa agatctgtac gccaattgcc tggaggtaaa agaacagcgc
      841 ttcacgcccg ccattggtgt tgtaaaacgg ccggaagtca tgctgttcaa acatgcgcaa
      901 aatgtccagc ccattagcag cctcatcgcc atgcaatccc ggcggcaaac tcggcagttg
      961 atgcagccgc ataaagagcg ccagaagcag aaccccgacc acacccagct gataacgata
     1021 tctaaggatt atgcggccca acggctttat catcgaaaaa ccacatgctt atacatataa
     1081 aaactccagc tcaggctgca cagcgttccg gcaagcttag ctccattatt cagggcaaag
     1141 ctatgggaaa tcaagcggtt aagccgtgtc agctttaata gatggcgaat cagcctgtcg
     1201 ggccaactcc agcgaaccgc taaaagatac agtacgccag tctggataat atacagccca
     1261 aaagctgtta ccgcaaaaaa cagtaccgcc tgaacaaccg gatttccggt tccggattca
     1321 aacaccgctt ggcggttggc aaaaaaactg aagatcattg cggtggttgt tgaaatgatg
     1381 ttggcggtca ctttgtgcat gtgaaagcgc ttactcgtca aaatattaaa aatggtaaag
     1441 tcgatgatgg tgttaagcac tccgacaatg ccaaattttc ccacctggct aagatcgcca
     1501 tggctgaaag gtatcattaa ataagcataa ccgtaccagg tatagatgta aagatagctt
     1561 acatgttatg gccggcgtgc agccgatcaa catcacggaa caataactgc tcgggatgga
     1621 agtagagttc aatttcaccg gtcggaccgt tacggtgttt acgaaccaaa atttgcgccg
     1681 agcctggctg ttcggcctct ttcccccggt aataatcttc acggtaaata aacatcacca
     1741 cgtcggcatc ttgctcaatt gatcctgatt cgcgcaggtc ggaaagcatt gggatcggcg
     1801 gcgtccgtga ctctaccgaa cgcgataact gcgagagcgc cagtaccggc acattcagtt
     1861 cacgggctaa tccttttaag cctctggaga tttccgaaac ctcttgaacc cggttgtcac
     1921 tgcctcccgc gttgcggccg ctgatcaact gtaagtagtc gacaataatc agcccaagat
     1981 catgctcact ttgtaatcgg cgggctttgg tgcgcatctc cataacattg gttgaagccg
     2041 aatcgtcaat atacaccggc gcttcactta gaaccgccat agcggtgtta attttcggaa
     2101 aatcgctgtc atccagccgt ccgttgcgca gcttccaggc atcgatcccc gactcgctcg
     2161 caagcagccg gtcgattaac tgctccttac ccatttccag tgagaagata ccaaccggaa
     2221 ttccttcctt aaccgccaca tgatgagcaa tgttcatggc cagggtagtt ttacccatgc
     2281 ttggccgtgc agccaaaata atcagatcgg acttttgcaa tccggccagg acgccgtcta
     2341 gcgatttaaa gccggttggc acgcctcgca aactgttctt atccttatga agttcatcga
     2401 gtcggtcaaa cgactcagcc aaaactgagc taatcggaat aaagttttgc ttcaggtgct
     2461 tttgcgatac cccgaacagt gtttgctcgg ccttatccag cagtccgtcc agcggtgtac
     2521 tttcatcatg ccctagtccg gcaattgtac tagccgccga aatcaaccgt cgcagcgtcg
     2581 ccttgtgcgc cacaatccgg ccataatgcg ctacgtgcgc cgccgttggc acttgctcca
     2641 ccaactcggt taagtacgcc gaaccgccaa ttttttctag ctccttcacg ctttcaagcc
     2701 ggttggacaa ggttagcaga tcaataggct gacgcgcttc ataaagatca atcatcgccc
     2761 ggtagacaat accgttacgg tcaacgtaaa aatcgtcggc agccacaata tcggcaacct
     2821 taaccagtgc ttccttgtct accaagatgc caccaagcaa tccggcctcg gcttccaggt
     2881 tatgcggtaa aatggttaag ggtttttgag ttgtttttgg agtggcaaca ctactcatga
     2941 ataacctcgc ctcctaaaat ttccagtgct gaactcatca gctcagccga tgaatccgcc
     3001 gtggcaggca cagtagcggt agccgcttct aactgaggca taactcgcaa cttgcgcccg
     3061 tatacccgcg ataaggtttg ttcaatgatt tccaagtttt tgggttcttt caggcggtct
     3121 ctaaaaaagc taaatttgca gccaatggta acgcggtcat tatcaaattc taaactgcac
     3181 gaccgaagca aggcatagag ggaattatta cgctgcttaa tttgggttaa ggctttcacc
     3241 cataagtccg gatcgggcac gctatcaagc ggctcggctt gaagcgctgc cactgcggtt
     3301 ggagtgtcgc tgggtacggc cggttttgcc gacaccgccg taccgggcct gacttttgct
     3361 gccaccggat ggctgcttgc ggtatcagga gtagttagcg caaccaacgc cacctcgagt
     3421 gatatctccg gccagctgct cttcagtacc ggcagcaatt cctgaataac ctgggcggtg
     3481 cgctgcagct cgctatccgg tgcaccgtcg tgaaccagcg cctgcaactt gtggcgttca
     3541 aacataacca gctggttggt aagctgtccg gcctgtactc cctgtgcaat ggcttggtca
     3601 accgcctgca aagccgccac cgtatcttga accatgaggg ccgcatcaat cgcctccaga
     3661 agctctggat cgctgtgtcc cagcagctgg cgcgtaaccg ctaccgttag gggcgatgtt
     3721 ccgctgctgg ctatttgatc caataagcta attgcatccc gaaaaccgcc tcggctggtt
     3781 gttgctaaaa gctcaagcgc ctcagcctca atagtgattc cttcttgctt agcgatagct
     3841 cccagatgct gaagtagatc atcctgagta atcaacttaa aacgaaactg ttgtgtacgc
     3901 gaaatgatgg tgaccggtag tttatgagcc tcggtggtcg ccaaaataaa aatagcatgc
     3961 gccgggggct cctcaagagt tttcaaaagc gcattaaagg cttcagtggt cagcatgtgg
     4021 acttcgtcaa taatgtagac cttatagcgg cccatcgccg gagcactgga gatcttttcg
     4081 cgtagcgccc gcgcatcatc aataccgcgg ttactagctg catcaatttc tataagatca
     4141 agatgcgagc caatctcaat cttgcagtta ctgcaggtcc cgcacgggcg tttgccggga
     4201 tcctcactgg tgcagttagc cgcgcgggcc agcagccgag ccacactggt tttgccaacc
     4261 cccctggggc cggtaaaaag gtaggcgtga cttaagcggt cgctgctaat agcttgctgc
     4321 agtgtttcaa tcacatgtga ttggccaatt accgttgaaa aagaggcact gcgatacttt
     4381 cgatacagcg ctaattcgcc caagctccta catcccctac taaagcatta ttggagtcta
     4441 ttatagcgct agtagcaggt aaagaccaga tgctgttatg ctaaaaccgc ttcaattgcg
     4501 gcccattcgg cctcaccctc ttcggggata acaatggtaa tttgatcacc cggctgggcc
     4561 ttgtaatacg tgacactggc aaacaacaca cggtagtcgc ggccttgcgc ctcaacatag
     4621 tattggccgt ctttaaggtt aagtgtacca attagcttat ggcgctgcgc aggagcaatt
     4681 tgtttataca gaaaagatcc atcctcgcca atagtcagtt ttaggccgtc ctgctctacc
     4741 agttttgatt tggaagcata attggctggt accggataca gttttccgtc aggcccaacc
     4801 atattttgac catcaaaatg gccctcaatt actttactgc caacgggaat cgaagttaag
     4861 accgtgccgg cctcttcatc gcccaccaag ctcgccagca gctccttggc acctgccaag
     4921 ttggtttcag ctgtctcaat aagcttcttc agagttttca attgtttgtc ggctagctct
     4981 gccacaggtg tatgtccatt tgttttggta tgataggtta cccatattca accatagagt
     5041 gggtaaaagg taaagtatag agggttgtag cttgaaccgg actactgaag ttcgacggta
     5101 gcgccagcgg cttccagggc agctttagcc tcttcggctt cagccttggg aacattctcc
     5161 ttaatcggct taggagcacc atcaacaatt gccttacttt cggccaaacc ttggccggta
     5221 agctcgcgaa ccgccttaat cacattgatt ttctgctcgc cggcagctgc caaaatcaca
     5281 gtgtatgcgc ttttctcttc tgcagcttca gtctcaccgc caccgcctgc aggagccgcc
     5341 atggccatag gagcagccgc tgacacgcca taatgatctt ccagtacttt cactagctcg
     5401 ctgagttcaa gcacgctgag cttatcgatt tgccctacca gttccttaaa cttcttagga
     5461 atttccttag tctcttcttt tgatgcagtt tcttctgcca tgattatctc cttagttatt
     5521 ctttagattc tttaattgca ttaagtaccg taacgatacc acgtacattt gcgcccatca
     5581 ctcccacaaa actgctcagt ggagccgcaa ttgtgcccac caactggcca agtagttgtt
     5641 cgcgggtagg tagtgtggcc agtgccttga cttgcgctac cgacagcagc tcgccgtcac
     5701 cggtaacagc cccaacaatt tccagcgctt gatgttcctt agcgtactgg aaaataaccc
     5761 gggccggagc cacctcgtca ttggtgctga tagctagggc cattggaccg gtaaaggtgg
     5821 agggatcaac cccttttaag gccggtgtgt tcttggcagc aatgcgcagc agggtgtttt
     5881 tggtaacgcg gtagctcata ccttcagagc gcaacttgtc tcgcagttca ctaatctcgc
     5941 tcaccgacag accgcgataa tcggttagca ctactaactt tagctctgcc agttcatttt
     6001 gcagttggct gacggcttgt tctttttctt ggcgagtaat tgccatgagg gctccttaaa
     6061 caaaaaactt cggcgactag ccgaagaccg taatcattaa accatcatta ggttgaatgc
     6121 tacctcggga gccaattaag cctgctggcc gctccggtct tcagtaatct agggctatgg
     6181 tacccgataa tcagatgagc gtcaacccgc cttcggctgc aatggctgct taaacctacc
     6241 ttctttttat caagattagt gtcccaagga tactactaat tagattaaat gtacgtgcaa
     6301 tgaggggtgc aatagtatcc ttgggacact attttgttac agcatgtaca tgatgaaaaa
     6361 aaagccaaaa ccccggaggg tttcgctctg gtatagcagc aaaaccctgc ctccgaaaaa
     6421 acggaaattc cccgaggcaa accggtagtc ggtgcggatc aaatgggtgg tgatgtcgca
     6481 gtgcttccac gtctccacga tccccaggtc attcatgcac cgttgtacga cctgcatcag
     6541 caaacctcgc gttccatcca tccgtagttt agccatgctt atccttccaa atcgaggttg
     6601 gcccctggct aaggggactg gatcggtcca gtcttggcga atattatact ataatattca
     6661 cataattaca aataaaatgt aatgagtctg tggcggcagc tatattttga tatcgtcgta
     6721 ataccacgtt agtccggcta gctttgcctt aactgattgg gcagcggtgg tatcatcggc
     6781 tttgccggtt gccatgtccc caattaacgt gcccagttcg tgtgccccgt caacatctaa
     6841 tccacggctg gccagtgcag ccgtaccgaa acgcatgcct gaaggccgcc aggccgactt
     6901 ggtgtcaaaa ggcagcatgt tgcggttggt aataataccg actgcttcta agcgcttctg
     6961 aaaatctaaa ctatcaacct catcaggtag tttaatcagg cataaatgat tttctgtcgg
     7021 gcttacaact cccaagccgc cctccttgca tccatcttcc aaggcttttg aggtatccag
     7081 taccgtttga ctgtaggtct taaaatcaat gccatccggg tatttatcct cgcccaagat
     7141 ctctagtaac gactgcccaa ttgccaccat ttgttgcaaa tgtggtccgc cgctagtacc
     7201 gggaaaaatc gtcttattaa tatcaccaat ataatcattc ttggcaaaca gcatggcgcc
     7261 tcgtggcccg cgcatggttt tatgggtcgt catagaaaca aaatcagcac cagccgcccc
     7321 ggccttaaac ggcgaatcat ggagtccagc agcgaccaac ccattaatat gagcgatatc
     7381 cgcgagcacc agtgcaccag cctcgtgggc ggccttacac atggcttcaa actcatactt
     7441 gcgtggatat gccgagaaac caatgataac cagcttaggc ttaaagcttt ttagcttctg
     7501 catataatca tcactatcca cctcaaacgt tccgggcgtg acttctttta cgtcgtaggt
     7561 cccatgctca aagaactttt tgtaattgct ggtggcatgc aaatgggaaa gatggcctcc
     7621 attggctaaa cttaagctca agatcttatc gcccggctca agcatggcca agtacaccat
     7681 ggtattggcg ggcgagcccg aaagtacctg cacattcacg ccatactcgc cggtagagtc
     7741 atagacctca agcgcttttt gctgcaccag aagctccatt tcatcggcat tcgtaacgcc
     7801 ggcataatat cgtttaccag gagagccctc ggcatacttg gtgctccaaa ccgaacccat
     7861 tagccctaag gccttggggg aggggtagtt ttccgaagca attaagttta gtgtgtcgcg
     7921 ctggcgggca tactcgtcgt gggtaagttc aataaatcta ttcatacttc aattgtagtg
     7981 gctgagatga gatcgtgcaa aaatggtatg atttatacat gaggcaatat ttagatctct
     8041 tgcaagatat tcaaaaaaac ggcacgatta agggcgaccg caccggcacc ggcactacta
     8101 gcgtatttgg acggcaaatg cggtttaacc tgagcgaggg attccctgcc gtcaccacca
     8161 aaaagctgta cttgcgtagt attattcatg agctcttatg gttcctgcag ggcagcacta
     8221 acataaaata cctggtagat aatgatgtcc atatttggga tgaatggccc tatcgtgctt
     8281 atctccaaaa acaggaaaaa ccggtacctc cctccgacag tgaagaatgg cagatcggca
     8341 ttaaggaatt taccgaaaag attaaagccg atgatcaatt tgccaaagaa catggcgaac
     8401 tcggtccggt ctatggctat cagtggcgca gttggccgac accaagcggc gagcatgtcg
     8461 atcaaatcca aaaggccatc gatatgatta aaaccaaccc gaactcacgc cggattattg
     8521 tctcagcctg gaatgtggct gagattgacg aaatggccaa agccggtctc cctccgtgcc
     8581 atacgctctt tcagttctac gtagccgatg gcaagctcag ctgtcagctc taccagcgca
     8641 gcgccgatac gttcctggga gtgcccttca atattgcctc ctatgccctg ctgaccatga
     8701 tgattgccca ggtgacaggc ttgaagccag gagatttcgt gcataccttt ggcgatacgc
     8761 atatctatag caatcacacc aaccaggtca aggagcaact cagccgcgag cctcgcccat
     8821 taccgactat gaaggttaat cccaacgtga catcgatttt cgattttacg tttgatgact
     8881 ttgagctggt caattacgat ccccatccgg ccattaaagc tccaatagcc gtctagcatg
     8941 gggataaacc taattgttgc cgtcgcggaa aatggcgtta tcggtcgcaa cgacggcagt
     9001 ctgccctggc gcttgccggc agacttagcc cgctttaggc agattacctt acaccatcca
     9061 attatcatgg gacgaacaac ctatgagtcc attggccgaa ttttaccgga tcggcgcaac
     9121 attatcatta cgcggaattc ttcatttcat attgatggcg ccgaagtagt ccattcactt
     9181 gaaagtgcca tcgccttggc aagtgctggc gataaagaaa tttttattat aggcggcgga
     9241 tcaatttacg agcaagcgct gccgcgagcc gatgtgctgt atcttaccga ggtacatgct
     9301 gaacctgagg gtcaagttct atttgaatat aatcgggatg aatgggaaga agttgaacgc
     9361 gacgaccgcc ctgccgatga tctaaaccaa tacccttatt gctttgtaaa gttagtgcgg
     9421 aagtaatatt aaatttaacg cggcagcttt gccttaatcg ccgaccaaac cttttcgctc
     9481 acctctttct tggtagctac ttttccaccc tcggcgcatt caattaaatg gaatatcgtc
     9541 ggaaacagtt ttgccatttc taagtaagct tgctcggcct tttgtaagtg atgtaaatcg
     9601 tcttcatgaa tgtcgcgttt tttagtgcca aggtattccc gctgtttctt tttatcaact
     9661 agtttttgag caagcgctgc cggcatatga agcacaacat taagatccgg ccgcggaatg
     9721 ccgagcaagc caaattccaa gtcttcaatc cattcaaaaa aggctttgcg ctcctcgctg
     9781 tcggcaaaac gagcaccttg gtgggccata ttcgaaccga catagcgatt ggccaggaca
     9841 attttgccgt cggctaaagc ctcgcggata gcgaaaccag cctcaagtcg atccatcgcg
     9901 taataaagag acgcttgata aacgtttacc tcgtcgacac tgccatattt accattaaga
     9961 taccgctcaa cgaaatgcgc actcggtttg ccgtattgcg gaaaatcaaa aacggcaatt
    10021 ttatgtcctt ccttactcag gcgcttgtga agcagttcgg tttgagtacc tttgccggca
    10081 ccgtcggtgc cctcaatcac gataaataag ccgggctttg tcatcagacc ctcggcattg
    10141 aaattgtatc ggtatatgcc cgcggcagca tcatttccgg tttccaggct tttttaacct
    10201 cctgaataga gccttttacc tgatatttat cgctttcgcc ataggttttg tcggccttag
    10261 ccatatggta aaacaccagc tgagccaccc gcatgcccac gggaagcggc actgaaacat
    10321 cattatcatt ttgaatttcc atagtccagc gattaatata ccccggatcg ccccagccag
    10381 cgtccttgca taccacaatg ccgttgcgtc cccacgtgga acgggcttgc atggtagtag
    10441 tgccttcata agcaataccg gcaaattcaa cggtgtgcgc taaaatccgc tcatgcggcc
    10501 gcagcacaat aatcggatga ttcaggggga tgttcttcca aggacggcga ttgtgatgct
    10561 ccgcccagtc cttgttgctg atagctttga gcgggccttt aaaataatct tcaaccgaat
    10621 aggcgtcaaa ggggttgtag tagtcctggt tggtggcgct gtcggtaaca taaaaccact
    10681 cgccaaggcg aacatcgacg ctgctgccct tcacgttcgc gggctcaaac ggatcaataa
    10741 cgatctgtcc gctttttagc gcagccttaa tttcccggtc gctaaatact cccacgacta
    10801 ctccttaaaa cttttctcaa tcatatcacg agttaccgct tggggttagc ggtagcaatt
    10861 gcagcctgaa cgtccaattt aatccctggc cccatcgtgc tgctgagtgc tgctgactgt
    10921 acatacgtac ccttggctgc agctggtttg gctttcataa cagcatcaat ggcagtagtc
    10981 gcgttgctca tc
//
LOCUS       DASVGB010000108         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1212568, whole genome shotgun sequence.
ACCESSION   DASVGB010000108 DASVGB010000000
VERSION     DASVGB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212568"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1023)
                     /locus_tag="VF272_03415"
     CDS             complement(28..1023)
                     /locus_tag="VF272_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7259954.1"
                     /translation="MKTNSSPKQAIKVKKLRKSYGKLKVLDGIDFSVEQGSILALLGP
                     NGAGKTTTIRILSTLLLPDGGQASINGHDVVKESAAVRSNIGLTGQFAAVDEYLTGAE
                     NLYMMGRLYRLSVKDVKRRSSELLEQFDLVEASTRPVRTYSGGMKRRIDLAMSMIASP
                     PIIFLDEPTTGLDPRSRLTMWGMIKQLAKDGITILLTTQYMDEADQLADRIVVIDDGK
                     VIAEGTPDDLKARVGADRLEIIVSKNSNFEQARKIVDGESLQADPERRTLSVASKGGV
                     KTLKEVLQRLEAGNIEVESISLHRPTLDDVFLTLTGHAATKKAEDAAEKVKGKAS"
     gene            complement(1077..2519)
                     /locus_tag="VF272_03420"
     CDS             complement(1077..2519)
                     /locus_tag="VF272_03420"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7259955.1"
                     /translation="MKRITRYEIVAFITGFSLLAYELAAARILAPSIGSSTYVWTSVI
                     GVIIAALSLGYAAGGLVADKRVKRSDIAWLLLMAASAVVFTLLLSDTVLEVATSNVSD
                     PRLQGLLVATLLFSPASFLLGVISPYLARLRTISLETTGTSVAFLSAANAVGGIIGTF
                     SVGFIFFGYIGSKETLLFTTLLLIFCSLLFAPYRQIVKRLGVGSLISLFVFISFYQVQ
                     AEGVVADIDTPSARYTVQDISYNSQPTRILVSGPRAAQSGVYLNGSSDLVFAYTQQMA
                     VLVEQVQKKDRILILGGGAFTLPRYLAEKYPSSQIDVAEIDSKLVGVAKKYFNYRDRP
                     NIRVYDEDARAFLNANTTKYDIILVDVYSDSAIPFSVATREYAAKLAASLESDGIVFS
                     NIIGTDTERCRPLLRAIHGSYSASFRHYQLYPQGDRELALNQNIIFAYSNQPLLDDGA
                     LKRDLSGGMALTDNYAPVERLKLECQNSEY"
     gene            complement(2536..3105)
                     /locus_tag="VF272_03425"
     CDS             complement(2536..3105)
                     /locus_tag="VF272_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259956.1"
                     /translation="MTRMNPLGKPVYAFLAATILVLTAFTVYTSAVTIELLRSNIGGD
                     RLATTIVRAGENLNRPLVIEPLSGKAYIPAAKLVLPAATDSLGEVLYRYYPPVNGSTA
                     ELYLASKSDMRSAEIPLLSSKDVNGLFATVPTFQACGRGIHITFQPEKEGAAVATKRL
                     GSGTTAYFYDEPKCQNPDLLDYAKQITSY"
BASE COUNT          881 a          833 c          746 g          808 t
ORIGIN      
        1 agtagagggg tgagtggtat tctggcttca tgaggccttg cctttgactt tctcggccgc
       61 gtcctcagcc tttttggtag cagcatggcc ggttaacgtc aggaacacat catcaagtgt
      121 cggacggtgc aatgaaatgc tttcaacctc aatgttgccg gcttcaagcc gctgcaaaac
      181 ctccttcaag gtcttcaccc cgcccttgct ggcaacgctc agtgttcggc gctcgggatc
      241 ggcttgcagc gattctccat caacgatctt ccgtgcttgt tcgaaattgc tgtttttact
      301 aacaataatc tccaaacgat cggcaccaac ccgggctttc aggtcgtcgg gtgttccctc
      361 ggcaatgacc ttgccgtcat caataaccac aatacgatct gccagttgat cagcctcatc
      421 catatactgt gtcgttagaa ggatggttat accgtctttg gcaagttgct ttatcatgcc
      481 ccacatggtt agccgactgc gggggtcaag gccggtagtt ggttcatcaa gaaaaataat
      541 cggcggactg gcgatcatac tcatagccag gtcaatccgt cgcttcatgc ccccggaata
      601 ggttcttacc ggtcgggttg aagcctcaac caagtcaaac tgctcaagca actcactact
      661 acggcgttta acgtctttca cgctgagtcg atagagcctt cccatcatat agagattctc
      721 ggcaccggtc agatactcat caaccgctgc aaactggccg gttaaaccaa tattactgcg
      781 tacagcggca gattctttaa cgacatcgtg cccattaatg ctggcctggc caccatcagg
      841 aagcagtagc gtactaagaa tgcggatggt agtggtcttg ccggcaccgt ttggacccaa
      901 tagcgccaag atgctaccct gctcaacgct aaaatcaatg ccatcaagca ccttcagctt
      961 gccataggac ttgcgtaact ttttcacctt tatggcttgc ttgggggatg aattggtctt
     1021 cataccccta atcttagcaa atcccggaat gatataatac actatcgtgc atcaatctaa
     1081 tactcgctgt tttggcactc caatttaagg cgttcaaccg gagcataatt atcagttagg
     1141 gccattccgc cgcttaaatc acgcttaagc gccccatcat cgagtagcgg ctggttcgag
     1201 tacgcaaaaa tgatattttg gtttaaagcc aactctctgt cgccttgggg gtagagctgg
     1261 taatgacgga agcttgccga atatgagcca tgaatagccc gaagcaatgg gcggcaccgc
     1321 tcggtgtcgg taccaataat gttggagaaa acaattccat ccgactccag actggctgcg
     1381 agtttggcag catactcacg ggtagcaact gaaaatggaa tcgcactatc actgtaaaca
     1441 tcaaccaaga taatatcgta tttggttgta ttcgcattga gaaatgcccg ggcatcttca
     1501 tcgtacaccc gaatgtttgg gcggtcgcga tagttgaaat actttttggc tacccccacc
     1561 aacttggaat caatctcagc cacatcaatt tgggacgatg gatacttttc agccaaatag
     1621 cgcggcaggg taaatgctcc gcctcccaga attaatatcc gatctttttt ttggacctgc
     1681 tcaaccaaca ccgccatttg ctgggtataa gcaaaaacca aatcgctgga accatttaaa
     1741 tacacgccgg actgggcagc tcgaggaccg gaaaccagta tgcgggttgg ctggctgtta
     1801 taggaaatat cttggaccgt atatctggca gaaggtgtat caatatcggc tactacgcct
     1861 tcagcttgaa cctggtaaaa cgatataaat acaaatagag agatgagcga accaacgccc
     1921 aaacgtttca ctatttgcct ataaggagca aaaagcagac tgcaaaatat caacaataac
     1981 gtagtaaaca aaagcgtttc cttcgacccg atatagccaa agaaaataaa accgacgcta
     2041 aaggtgccga tgattccacc aaccgcattc gctgcgctca agaatgctac cgaggtaccg
     2101 gttgtttcta gtgaaatcgt tcgcagcctt gccaggtaag gactaattac accaagaaga
     2161 aagctggcgg gagaaaagag cagtgtagcc acgagcaggc cttgcaagcg cgggtcgctg
     2221 acgttgctgg tggcgacttc cagcacggta tcggaaagga gcagagtaaa caccaccgca
     2281 cttgcggcca tgagcaatag ccatgcaatg tcagatcgct taacccgctt atcggcaacc
     2341 aggccaccgg cggcgtaccc caatgaaagt gcagcaataa tgacaccaat gacactggtc
     2401 cagacatagg ttgaacttcc aatgcttggc gctaaaatcc gggcagccgc caattcatag
     2461 gccagcaaag aaaacccagt aataaaggct acaatctcat atctggtaat gcgcttcaca
     2521 gcacgacctg cgttattaat agctagtaat ctgttttgcg taatctaaaa ggtcgggatt
     2581 ttgacatttt ggttcatcgt aaaaatatgc cgtagtgcca ctgccaagcc gcttcgttgc
     2641 gacggccgca ccttcttttt ctggctgaaa tgttatatgt atgccgcggc cgcaggcctg
     2701 aaatgtcggc accgttgcaa acaagccatt cacatcctta ctactaagca agggaatctc
     2761 ggcactacgc atatcacttt tgcttgctaa atataattct gcagtcgatc cgtttactgg
     2821 cgggtaatac cgataaagaa cctcgcccaa gctgtcagtg gcggccggaa gcactagttt
     2881 tgctgccgga atataggcct taccagatag tggttcaata actagaggac gattaagatt
     2941 ctctcctgcg cgcacaattg tagttgctaa gcgatcgccg ccgatattgc tgcgaagcag
     3001 ttcaatcgta acggcgctgg tgtaaacggt gaacgctgtg agtacgagaa tagttgcagc
     3061 aaggaatgca tatactggct tacccaatgg attcattcta gtcatgctta aagtatacca
     3121 cctcaaaaaa aataatttaa catttcgagt tctgacggcc catcccctcc aataaacgcc
     3181 catatgctaa gtactacttt ccgaccccct ccataagcat gcctagtttg cgaaccgtat
     3241 tgatattacg aattgtcata tgcttata
//
LOCUS       DASVGB010000109         5551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_956977, whole genome shotgun sequence.
ACCESSION   DASVGB010000109 DASVGB010000000
VERSION     DASVGB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956977"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF272_03430"
     CDS             complement(<1..903)
                     /locus_tag="VF272_03430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7259957.1"
                     /translation="MAGETYGRIRSINNLTTEVVWVGDKPAPRDLLRLTDDPSILFEV
                     QSFNEFSHAICINLGASPKAVRGAMLEPVGDTITVPVGKASLGRLFNALGEPVDGLAD
                     LSKEPRRSIYKPTQPSQTIKPLKPEILETGIKVIDFFTPFVKGRKIGIVGGAGVGKTV
                     LMMELIHNVAQDGTSLSFFAGIGERIREGHELYETLQESKLMDKSVMFYGQMNESAAL
                     RSKVGLTAVTLAEYFRDEENRNILFFVDNIYRHLQAGNELSTSLGQIPSEGGYQATLF
                     SDLKQLEDRLFSNEHGSITSVQTIY"
     gene            complement(905..1756)
                     /locus_tag="VF272_03435"
     CDS             complement(905..1756)
                     /locus_tag="VF272_03435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX7259958.1"
                     /translation="MRRPIVIQQDLERIGTIQDLTSIFEVIASIHISQIKDRVVSSTK
                     FFNELWGVYSQLRSGEDDQMLSIQRNGRTALVVITSDGGLIGDIDERLVREMQKHDVG
                     DKTDVYAIGAHGAQLLSQRGVKIAKVFMAPNIEKGESVHELAKVFGAYERVTVYYQTY
                     LSLMRQETATIDLFSVVQHLGKGSVDEKDLITSHTYILEPSPKEIISYMEAVMVEIAL
                     GQVVLESKLAQYASRFNAMYAAKSKAKEMRDDLALMLHRAKRASGDERIKEILSGMKM
                     LKKREAK"
     gene            complement(1753..3180)
                     /locus_tag="VF272_03440"
     CDS             complement(1753..3180)
                     /locus_tag="VF272_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HEX7259959.1"
                     /translation="MFENEQFARLIAEGKPSGEVVAIKELLTIIRGLEGAAINSLIMF
                     ENGDRGMVRSVKDSLVEVLTFAAHGSKLGSLAVLEREVLQTAVGEGLIGRVVNVLGEP
                     LDGKGSIATSAEAPVFATAPGIIERQALDKQLVTGVAIVDSLFPFVLGQRIAVLGDNK
                     SGKSTFMRQLTQQQAGEDGRIIIYVLIAKRQVDVDTLIAELNETGAIQHAIIVVASSF
                     DALSQSYLAPYVANAMGEYLWKAGRHVIIIYDDLTNHAKVYREISLLAEVSPGRDSYP
                     GDMFYAHSSLLERAGKLKDGGGTFSAIPVILTPNDDITAYLSTSIMSITDGQIIFDLQ
                     SFRKGVRPAVNVGLSVSRVGGRVQNKDWKKLSGQLFRKLADYRQAAEFSQFGSELSPE
                     AQADLALGRQLYEVYKQLPRELFDINTQYLMLATAMAGAGKVSLNVVLLKQEAQKRAA
                     EMAPDADPAPLVQQLLQLATVQVAK"
     gene            complement(3173..3640)
                     /locus_tag="VF272_03445"
     CDS             complement(3173..3640)
                     /locus_tag="VF272_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259960.1"
                     /translation="MKPNLPEEVYSVDQILAVSEELHSYSVYLAAQRVQGVAGEPFIL
                     SSRAEAAVKRIPEDQRTDAGIEAYRLQLEGLATTAPAVTLVLAAQAKAGLKQELVNWL
                     RTNVHPDLLVNFRANPNIAGGIVIRTINHVYDCSFRQALLAHPEKFTKALDRV"
     gene            complement(3630..4274)
                     /locus_tag="VF272_03450"
     CDS             complement(3630..4274)
                     /locus_tag="VF272_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259961.1"
                     /translation="MTEVTIVGLLILFSVLVGAIVMWFEKRKKLNKGGHAHNQEAPAE
                     PAEPAEPDAPPINLPELSTAERERLEKLASQAFEAAVASATDRFGKNLDETSGRLNDL
                     VTRLTTGIVEKELEVYRANVDEARKAALASLANMQNIVETQQQTLQKDIDRALAERRD
                     YLIERLDKKLGEAVAAYLVDSLGQAADLGAQRAFLLESLERHKADLKNEFSDET"
     gene            4381..4821
                     /locus_tag="VF272_03455"
     CDS             4381..4821
                     /locus_tag="VF272_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002033161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HEX7259962.1"
                     /translation="MPSQARQLISYSVYGAFAAALVATLGSLFLSEIKHLPPCVLCWY
                     QRICMYPLVIILGVGILRAERAIYHYVLPLATIGWMIALYHTLIQWKIIPDDLSPCVQ
                     GVSCTTVQVNLLGFITIPFMSLVAFSIILISMITLIMHERSARP"
     gene            4818..5447
                     /locus_tag="VF272_03460"
     CDS             4818..5447
                     /locus_tag="VF272_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7259963.1"
                     /translation="MSREAKILLGIVFVITAGMVALFVIGGDPAPSSEGTDSSKLVRE
                     SSHKTGTGPIQVVEFGDYQCPACFQAYPVIQKIKQDYNGKITFSFRNFPLQQHKNAVV
                     AARAAEAAALQNKFWEMHDELYQHQEEWAQVSNPSTFFDRYAKEIGLDVAKFKQDGAS
                     AAIKKIIDSDQSDGVAVDVQATPTFFVDGVKADAFDYNTLKTMIDSAKK"
BASE COUNT         1360 a         1524 c         1268 g         1399 t
ORIGIN      
        1 gtaaatggtt tgcacgctgg taatggaacc atgttcgttt gagaaaagac gatcttccag
       61 ctgtttaagg tcagaaaaca atgtcgcctg gtagccaccc tcactcggaa tttgacccaa
      121 gctagttgaa agttcattac cggcctgcaa gtggcggtaa atgttgtcaa cgaagaacaa
      181 gatgttgcgg ttctcctcat cccggaaata ttcggctaag gttaccgccg ttaaccctac
      241 tttggagcgc agcgccgccg actcattcat ctgcccgtag aacatgaccg acttatccat
      301 gagcttactt tcctgcagcg tttcataaag ttcatgcccc tcgcgaatcc gctccccaat
      361 accggcgaag aacgataaac tggtgccatc ctgggccaca ttatgaatta attccatcat
      421 gaggaccgtt ttaccaacac cggcaccacc gacaatacca atcttgcggc ctttcacaaa
      481 gggcgtaaag aagtcgataa cctttatacc ggtttccaga atttccggtt tcaagggttt
      541 aatggtttga ctcggctggg ttggcttata gatggagcgg cggggctctt tgcttaaatc
      601 agccaacccg tcaaccggct cgccaagggc attgaagagc cgccccaagg aggctttgcc
      661 aaccggaacc gtaatcgtat cgccaaccgg ctcgagcatt gcaccgcgca cggctttggg
      721 gcttgctccc aaattaatac aaattgcatg cgagaactca ttaaatgatt gaacttcaaa
      781 caggatcgat gggtcgtcag tcagtcgtaa taaatcgcgc ggcgccggct tatcgcccac
      841 ccataccact tcggtggtta ggttattgat tgacctaatc cggccgtatg tttcgcccgc
      901 catgctactt tgcctccctt ttcttgagca tcttcatacc acttaaaatt tctttgatgc
      961 gctcatcacc cgacgcccgc ttggcccgat gcagcattag tgctagatcg tcacgcattt
     1021 ccttagcttt gcttttggca gcatacatcg cattaaaccg actggcatac tgagccaatt
     1081 ttgactccaa caccacctgg cccagcgcga tttccaccat aacggcctcc atataactaa
     1141 taatttcttt cggtgaaggc tccaaaatat aggtatgact ggtaatcaag tctttttcat
     1201 caacacttcc cttccccaga tgttgaacca cagagaataa atcgatagta gcggtttcct
     1261 gccgcataag cgacagatag gtttggtaat agacagtgac acgctcataa gcaccaaata
     1321 ccttggccag ctcatgaacg ctctcccctt tttcaatatt aggggccata aagactttgg
     1381 ctatcttcac cccgcgctgg cttaaaagct gtgcgccgtg agccccaatg gcgtatacat
     1441 cggttttatc acccacatca tgcttttgca tttcgcgcac caaccgctca tcaatgtcgc
     1501 cgatcaagcc gccgtccgaa gtaataacca ccagggcggt ccggccatta cgttgaatgc
     1561 ttagcatctg gtcgtcttcg ccgctgcgca gctggctgta taccccccac agctcattga
     1621 aaaacttggt agaagacacc acgcgatcct taatttgcga aatatgaata ctggcaataa
     1681 cctcgaaaat cgaggttaaa tcctggatag ttccaatccg ctcaagatcc tgctgaataa
     1741 caattgggcg cctcattttg ccacctgaac cgttgctagt tgcagcagct gttggaccag
     1801 tggtgccgga tcggcgtcgg gcgccatttc cgcggcccgc ttttgggctt cttgtttcag
     1861 caacacgacg tttaacgaca ctttaccagc tccggccata gcggttgcca gcattaaata
     1921 ttgggtatta atgtcaaaca gctctcgagg cagctgtttg tatacttcat aaagctgacg
     1981 gccgagcgct aagtcggctt gggcttccgg tgaaagctcc gaaccaaact gagaaaactc
     2041 ggcggcttgg cggtaatcgg ctaacttgcg aaagagctga ccgctcagtt ttttccaatc
     2101 cttattttgg acgcggccgc ccacacggga aacacttaaa ccaacattta cagcagggcg
     2161 cacgcctttg cggaacgact gcaagtcaaa aataatctgc ccgtcggtaa tcgacataat
     2221 actggtggaa agataggcgg taatatcgtc gtttggcgtc aaaattaccg ggatcgcgct
     2281 aaaggtaccg ccaccgtcct tcagcttgcc ggctcgctcc aacaaagagg agtgggcata
     2341 aaacatatcg cccggataag agtcgcgccc cggagaaact tccgccagca atgaaatttc
     2401 gcggtacacc ttggcgtggt tggtaaggtc atcataaata ataatcacgt gccggccagc
     2461 cttccacaaa tactcgccca tggcattcgc aacatacggt gcaagatacg attggctcag
     2521 cgcatcgaaa ctggatgcca ccacaataat ggcatgctga atcgcgccgg tttcattgag
     2581 ctcggcaatc agagtgtcaa catcgacctg acgcttggca atcaggacat aaataataat
     2641 ccgtccgtcc tcacccgcct gttgctgggt aagctgacgc ataaaggtac ttttccccga
     2701 cttgttatcg cccaatactg cgatgcgctg tcccagcacg aaggggaaga gcgagtctac
     2761 aatcgccacg ccggtaacga gctgcttatc gagcgcctga cgttcaataa taccaggggc
     2821 cgtggcaaat accggcgcct cggcactggt agcaattgat cctttaccat cgagcggctc
     2881 gcccaagaca ttcaccaccc gaccaatcag cccttcaccg acagcggttt gcagtacctc
     2941 acgctcaagt accgcgaggc tcccaagttt ggacccatgg gcggcaaagg ttaggacttc
     3001 gaccagcgag tctttaaccg accgaaccat accgcgatcg ccgttttcga acatgatcaa
     3061 actattaata gccgctccct ccaacccgcg aatgatagtt agcaactctt taatagccac
     3121 cacctcgccc gaaggcttcc cttcggcaat caaccgggca aactgttcgt tttcaaacac
     3181 gatctaaagc cttggtaaat ttttccggat gcgccagtaa cgcctggcgg aaggagcaat
     3241 catacacgtg attgatagta cggattacaa tgccaccagc aatattggga ttagcccgga
     3301 aatttactaa taagtcaggg tggacgttgg tccgcagcca atttaccagt tcctgcttaa
     3361 gtcctgcttt tgcctgtgcc gccaacacca gtgttaccgc cggggcagtt gtcgctaaac
     3421 cttccagctg tagccggtag gcttcaattc cggcatcggt tcgctgatcc tccggtatac
     3481 gttttacagc cgcttcagca cgcgaagaca agataaatgg ctcgccggcc actccctgaa
     3541 cccgctgtgc tgctaaatac acgctgtagc tatgcagttc ttccgatacc gctaagattt
     3601 gatctaccga atatacttct tccggcaggt taggtttcat cgctgaactc gttctttaaa
     3661 tcagctttgt ggcgctccag actttctagc aaaaaggccc gctgggcgcc caagtctgcc
     3721 gcctgaccca agctatcaac caagtatgct gctaccgcct cgcccaactt tttatcgagt
     3781 cgctcgatta aataatcccg gcgctcagcc agagcccggt cgatatcttt ctgtaatgtc
     3841 tgctgctggg tttcaacaat attctgcata ttcgctaaac ttgctaaggc tgctttgcga
     3901 gcctcatcga cgttggcgcg atacacttca agttcttttt cgacaatccc ggtagtcagc
     3961 cgcgtgacta aatcattaag ccttcccgag gtttcatcaa ggttcttccc aaatcggtcg
     4021 gtggcactgg caaccgctgc ttcaaatgcc tgagaagcca gcttctcgag ccgctcccgt
     4081 tctgccgtgg acagctcggg aagattgatt ggcggagcat ccggttcggc aggctcggca
     4141 ggttcagcgg gagcttcctg attgtgagcg tgcccgccct tgttcagctt ttttcttttt
     4201 tcaaaccaca tcacaatcgc gcccactagt acgctaaata atatgagcaa tccgactatt
     4261 gttacttctg tcatcgccac ctctaacaca agctttttat aagccgtgtc atgcttatgt
     4321 cattacccta gtattatagc agttacgctt aataattaca gctgatgcta tactaatttc
     4381 atgccttccc aagcccgtca gcttatttcc tactcggtat atggtgcgtt cgctgctgcc
     4441 ctagtggcta cattgggaag cctcttcctg agcgagatta aacatttgcc tccgtgtgtg
     4501 ctgtgctggt atcagcgcat ttgcatgtac ccgctggtta ttattctggg ggtcggaatt
     4561 ctgcgcgccg aacgtgcaat ctaccattac gttctaccgc tagccacaat cggctggatg
     4621 attgcgcttt accataccct gattcagtgg aagattattc ccgatgacct ttctccctgc
     4681 gtgcaaggag tttcctgtac caccgttcag gtcaatttgc tcggctttat caccattccc
     4741 tttatgtcct tggttgcctt tagtatcata ctgatatcga tgataacgct gattatgcat
     4801 gaaaggagcg cacgcccatg agtcgtgaag ccaaaatatt actagggatc gtattcgtca
     4861 ttacagctgg catggtggcg ttgtttgtga ttggcggtga ccctgcccca tcctcagagg
     4921 ggaccgactc ctccaagctg gttcgtgaat ccagccataa aacaggtacc ggccccattc
     4981 aggtagtcga gttcggcgat tatcaatgcc cggcttgttt tcaggcctac ccggtaatcc
     5041 agaaaatcaa gcaggattat aacggtaaga ttacatttag cttccgcaac ttccccctgc
     5101 agcagcataa aaatgctgtg gtggcagccc gtgcagccga agccgctgcc ctccaaaaca
     5161 agttttggga aatgcacgat gaactgtatc aacatcagga ggaatgggcg caagtttcta
     5221 acccgagcac tttctttgac cgttatgcca aggaaatagg cttagatgtt gcgaaattca
     5281 agcaagacgg cgccagcgct gcaattaaga aaattattga tagcgaccag tccgacggtg
     5341 ttgcggttga cgtgcaagct acaccaacct tttttgtgga cggggtgaaa gccgatgcgt
     5401 tcgattacaa cacgcttaaa accatgatcg atagcgccaa aaaataagag ctataaaaat
     5461 tagtacgtaa agttagcaga tcgcacaacg aagatcttcg ccgccttggc tataatgctc
     5521 ctatcataag taacgataag ggggtaccag t
//
LOCUS       DASVGB010000110         8643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_718756, whole genome shotgun sequence.
ACCESSION   DASVGB010000110 DASVGB010000000
VERSION     DASVGB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718756"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(824..2485)
                     /gene="groL"
                     /locus_tag="VF272_03465"
     CDS             complement(824..2485)
                     /gene="groL"
                     /locus_tag="VF272_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514589.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7259964.1"
                     /translation="MAKKIIADSEARKRVLEGAKELYDAVKVTMGPKGRNVVIGKSYG
                     GPQVTHDGVTVAKAVELEQKRDDESLGRGIGAELVKQAASKTNDNVGDGTTTATILTY
                     QILYEANKLIAAGHNPMDLKKGLDRAAAKVQGEIAGLSEKIAGNKAKIAQVATVSAGD
                     SEIGNQIADVIEAVGEDGSVTVEQGQGLGLESEIVEGFKIDRGYVSAYMVTDTAHMEA
                     VYEKPLILITDKKISSIQDILPLLEKVAQGGKKELVIVAEDVDGEALATLILNRLKGV
                     FNALAIKAPSFGDRRKEVLQDIAVLTGGTVISEEQGITFENATLDMLGTARKVIATKE
                     DTTIIEGGGKPTEVQQRMVQMKAQAENATSEFDKEKIDERLAAMAGKVAVIKVGGATE
                     TEISEKKDRVDDAVAATKAAVAEGIVSGGGVTLVDLSKRINASAASVVDSESAGSVIL
                     KEALLHPFRQLMENAGLNPDAKLAKVLESVKPGFGFDVNKPEKLIDLRATGIIDPAKV
                     TREAIQNAVSIAGTAMTMGALVVDIPEEKSAGGGAPDMGGMGGMY"
     gene            complement(2508..2771)
                     /locus_tag="VF272_03470"
     CDS             complement(2508..2771)
                     /locus_tag="VF272_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7259965.1"
                     /translation="MTLQPLADRVVAKPLEAQDKTSSGFYVPDSAKEKPQMAEVVAVG
                     KEVKEVKVKDHIVYEQYKSETIKIDGVEMLIVKEEHVLAVAKR"
     gene            complement(2893..3528)
                     /gene="cmk"
                     /locus_tag="VF272_03475"
     CDS             complement(2893..3528)
                     /gene="cmk"
                     /locus_tag="VF272_03475"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7259966.1"
                     /translation="MTKLIIAIDGGAGTGTSTVARGVAERLKLPHLNTGELYRGITFL
                     VLQNGINPIDSAEILKLAKTIDFGFDDSNVVTVNGYDVRGKLHQEDINEQVAVVSSHE
                     PVRDEILHLQRNFANEHGVVMEGRDITTRIFPETPYKFYFTCDPEERVRRVNAGGRAH
                     ETVASLLKRDAADAAKVVGTFKQADDATVIDTTNRTIDEVVSFIVELVKPN"
     gene            complement(3525..4832)
                     /gene="murE"
                     /locus_tag="VF272_03480"
     CDS             complement(3525..4832)
                     /gene="murE"
                     /locus_tag="VF272_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HEX7259967.1"
                     /translation="MFRRIVKTLIPRRLFRRIEPYGHLVETFLANLYYGFPARGLKVI
                     GVTGTDGKTTTTTLIWTMMKQAGYKAGYMTTIGYGTPQWQRYNKVHMTSMQAFPLMSR
                     IKAMRAAGIEWLVLETTSHALAQNRVWGVPFSVAVMTNVNHEHLDYHGTFERYRDAKR
                     RMFQLAADNRAGLQTGIINAEDPSADLFAADVPSSILYGIDGGSVKATNITATPTGSE
                     YTVKRGNSTLHINLHLPARFNVYNSLAAVSTGIAVGLTNKQIEQGIAALGNVEGRMTK
                     VEEGQDYHVIVDFAHTPASFEKLLSDMRSQVKAPQKLIVMFGSAGRRDEAKRAIQGGI
                     AGHYADIVVITEEDDRDIDGQGIVEQIATGAETSGKVRDKNLFLVHDRSEAIAFAMQQ
                     ASSGDTVMLLGKGHETTIERGGGEEPWNELAEAKQAIHNKRKT"
     gene            complement(4870..5166)
                     /locus_tag="VF272_03485"
     CDS             complement(4870..5166)
                     /locus_tag="VF272_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259968.1"
                     /translation="MRVVIHLSFSVKQAAELQAQKLGYASLAGLLKVTATLLGDRVEA
                     AALPEQPALLSSLKMMGKLPHPDQFAEWPPHVCPTIATTDPDLYAFYIQTGIIR"
     gene            5390..5620
                     /locus_tag="VF272_03490"
     CDS             5390..5620
                     /locus_tag="VF272_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259969.1"
                     /translation="MKLNALLQEPHKIGWALFALSASFLLAVIVAGSLGMTSLAMMLS
                     QIAQPICASAIGVLVYLAMVARRHEKTSLEGS"
     gene            complement(5623..6654)
                     /locus_tag="VF272_03495"
     CDS             complement(5623..6654)
                     /locus_tag="VF272_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX7259970.1"
                     /translation="MLLKPATADELAQWDALIAANPDGGNVLQAKAFGETKSRHGWKA
                     NYLMFEGTAILALVRNVPMFGELWYIPKGPGVQDIQQLKRITAELKKTGAFMVKIDPE
                     LPKATKLSALASAGLVRASRDIQYNIATVVVDLTPGEDDILASFKQKTRYNIRLAVKK
                     GVKVETVETTLESVDRLYEMSKTTYERAGVYVRNKQYFFDFWDLHSKQRTGQMFFATY
                     EGKPVAGAFITYLGESALYKDGASYRDHSELQAPYLMQWEIMRWLKAKGIKEYDLHGV
                     PPAAEIDNTNHPLAGLARFKRGFNPEITEYAGTYDLVVDHGKYQLWNQIGERLAMAYQ
                     SRRNHRLFY"
     gene            complement(6654..7619)
                     /locus_tag="VF272_03500"
     CDS             complement(6654..7619)
                     /locus_tag="VF272_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX7259971.1"
                     /translation="MKHGLPPNDWDHTLQMLGGSILQSRLWMLFQQHLGREIVWEADE
                     GWQWSASIRGSHGLRYLHCAYGPAARDEAALSTAIMSAAHAAKELGIDFVRVEPQRSI
                     SITELVTLRARNIAEVDPQHTRIIDLSKSEAELRTDLASGHRNLVNGTKRRRIVIRKT
                     DKAEDFDVLLKMLADTAKRSHVTFHPSEYFQNLWEVFQPHDAAKLYLAEVEGKPVAAA
                     LFYDWSGIRYYAHAGAYQEENRRVKASVSLVWQAIVDAKVEGLKQFDLWGVASAGDSS
                     HHLASLSRFKEAFGGQQADYAGTWDIPVKPVKYAAYSLYRRVRGR"
     gene            7660..>8643
                     /gene="tsaD"
                     /locus_tag="VF272_03505"
     CDS             7660..>8643
                     /gene="tsaD"
                     /locus_tag="VF272_03505"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7259972.1"
                     /translation="MNILGIETSCDETAAAVVKDGRIILSNVVASQGDLHAQFGGVVP
                     EIAARSHIEMILPIVKKALDEAETDWNAIDAIAVTRGPGLIGSLLIGILAAQTLATLK
                     NMPLIPINHIEAHTYANFLLPAPLSFPLLSLTVSGSHTQLVLFTDHGQYRVLGKTLDD
                     AAGEAFDKVARMIGLSRSSGAAIEVAAAHGNNQAFAFPKPKLGKESFNFSFSGLKTAV
                     LRTAQAAVGRDHRTLSHELPALLRDSQRNDLAASFQKTVVEILAGRLLQAYEAHSPSS
                     VVIGGGVAANQALRSKVATLLPVTVGYPPLDLCTDNAAMVASLGYFQLQSGR"
BASE COUNT         2018 a         2392 c         2074 g         2159 t
ORIGIN      
        1 agcacgtcgg gcctccattc agtatgccta gtattgtaac ggtcgtgact cagggcgtcg
       61 agaaaacggg tgcttaaggg tgataattgc aggtgttaga cacaccagaa cacagggatg
      121 gtgaaatgcc ccaaggtggg acgcaggtgg cgggcctcta gaagcccgcc accatgatgc
      181 gcccagcgcc ggtgcatggg tgggccggcg aagattcagc tcccctaggg acgcggggcg
      241 gcaagggcct ggatcacgga gacccttgcc gccctggggg cgtgcgccct tacgccgatc
      301 actggagggc cgtgaccggg taagttagcc gcgtgaagcg gcaaagatgg acgcaggtga
      361 cggagccctc gcatccgcca ccatgatgcg cccagcgccg actaccggga gagcgtcgac
      421 gaagactgat aagacaacac ggaggcggcc ggggccaccg gagaggaggg ttacaggaca
      481 accccgaccg ccatccgcga gaccaggact tgcactacct catcgcaacg actccctgag
      541 tgtcgtgcgg cttgcacacg acggacggaa aacaacaccg cccaagccgc gcacgctttt
      601 tggttccgcc gagggcagaa cgaagagcag gcccgacggg tcatccggca tgatgtccca
      661 tcttgcatat tggtatgttc tggggtgtca atgaaaggct gcaacagact aaaagcctgc
      721 cacaactgtg aggaaaattg tagtgatact ttttcataac gtcaaccaaa aagagccctt
      781 aataaggctc ttttcagact ctacgctcag gcttttgaag gagctagtac atgcccccca
      841 taccgcccat gtcaggagcg ccaccgccag cagacttctc ttccggaata tcgacaacta
      901 acgcccccat cgtcatagcc gtaccggcta tcgaaacggc attctgtatc gcctcacggg
      961 ttaccttggc cggatcaatg ataccggtgg ctctaagatc aataagcttt tccggcttgt
     1021 taacatcaaa cccaaatccc ggcttgaccg actccagcac tttagccaat tttgcatccg
     1081 ggttcaagcc ggcattctcc ataagctggc gaaatgggtg cagcagcgcc tctttcaaaa
     1141 taaccgagcc ggccgattcg gaatcaacca cgctagcagc tgaagcgttg atgcgcttac
     1201 tgagatccac cagggtgact cccccgccgg aaacgatacc ttcggcgacg gctgccttgg
     1261 tagcagctac ggcatcatcg acgcggtctt tcttttcgct gatttcagtt tcggtagcgc
     1321 cgcccacctt aatgacggcc accttgcctg ccatagcggc taaccgctca tcaatctttt
     1381 ccttgtcgaa ctctgaagtg gcattttcgg cttgggcctt catttgcacc atccgctgtt
     1441 gaacctcggt tggcttgccg ccgccttcga tgatggtagt gtcttcctta gtagcaatga
     1501 ccttgcgggc ggttcccaac atatcgagcg tggcgttttc gaaggtgatg ccctgctctt
     1561 cactaattac ggtaccacca gtgagtacgg caatgtcttg caatacttcc ttgcggcggt
     1621 caccgaagct tggagcctta atcgctaaag cattaaagac gcctttaagg cggttaagaa
     1681 tcagggtggc tagggcttcg ccgtcaacgt cctcggctac aatcaccaac tcttttttgc
     1741 cgccctgagc gactttctcg agcagcggca ggatatcttg gatgctggag atctttttgt
     1801 cggtaattaa aattaacggc ttttcataga ccgcctccat gtgagcggta tcggtaacca
     1861 tgtaggcact gacgtagccg cggtcaatct tgaatccttc aacaatctca ctttccaatc
     1921 ccagaccttg accctgctct accgtaacgc taccatcctc gccaaccgct tcaataacat
     1981 cagcgatctg atttccaatt tcggaatcac ccgccgaaac ggtagcaacc tgggcaatct
     2041 ttgccttgtt accggcaatc ttttcgctta acccagcaat ttcaccctgg actttagcgg
     2101 cggctctatc cagccctttt ttaagatcca ttggattgtg gccggcggcg atcagcttgt
     2161 tggcctcata cagaatttga taggtaagaa tggtggcggt ggtagtaccg tcgccaacat
     2221 tatcattggt tttagatgcg gcctgcttta ccagctcggc accgattccc ctgccgaggc
     2281 tttcgtcgtc acgcttttgt tcaagctcaa cagccttagc aaccgttacg ccatcatgcg
     2341 ttacctgcgg gccgccgtaa cttttcccaa taaccacatt tcggcctttt ggacccattg
     2401 taactttaac ggcatcataa agctccttgg ccccttctaa tacccgcttg cgggcttcgc
     2461 tgtcagcaat aattttcttc gccataattt caattcctcc ggttagctta gcgcttggcg
     2521 acagccaaaa cgtgctcttc tttaacaatt aacatttcta cgccatcaat cttgatggtt
     2581 tcggacttat attgctcata gacaatatga tcctttacct tcacctcttt cacctctttg
     2641 ccaacagcaa ctacttctgc catttggggt ttttccttgg cactatccgg tacataaaat
     2701 ccgctgctgg ttttgtcttg ggcttctagt ggcttggcca ctacacggtc agctaatggc
     2761 tgtaacgtca tttgggtaac tcctccgctt atcacattta tactactaag tgtcattagc
     2821 actcacatgc ctagactgct aaggctatct taccgagagg atttttgcct gtcaacagcc
     2881 caaacgtaaa atttaatttg gcttaacgag ttcaacaata aagctaacaa cttcgtcgat
     2941 agtgcggttg gtcgtgtcga taacagtcgc atcatcagcc tgcttaaagg tccccacgac
     3001 cttggctgca tcagccgcat cacgtttcaa tagtgatgca acggtctcat gagcgcggcc
     3061 tccggcattc acccggcgca cccgttcctc cggatcacag gtaaaataaa acttgtaggg
     3121 agtttccgga aagatccgcg tggtaatgtc gcggccctcc ataaccacgc catgctcgtt
     3181 tgcaaagttg cgctgcaagt gcaggatttc atcacgcacc ggctcgtggc tcgacaccac
     3241 tgcaacttgt tcattaatgt cttcctgatg aagtttgcct cgaacgtcat agccattcac
     3301 cgttacgacg ttgctgtcgt caaatccaaa atcgattgtc ttagccagct tcagtatctc
     3361 ggcgctgtcg atcggattaa tgccattttg aagaacgaga aaggtaatgc cccgataaag
     3421 ctcaccggta ttaaggtgcg ggagctttaa ccgctcagct acgccccttg caacagtaga
     3481 cgtaccggtg ccggctcccc catcgatcgc aataatcaat tttgtcatgt cttccttttg
     3541 ttatggattg cttgtttcgc ttcggccagt tcgttccacg gttcttcgcc gccgccgcgc
     3601 tcaatcgtag tttcatgccc cttgcccaga agcataaccg tatcgcccga gctggcttgc
     3661 tgcattgcaa atgcaatggc ctcgctccga tcgtgcacta aaaacaaatt tttgtcacgc
     3721 actttgccgg aggtttctgc cccggtggcg atttgctcca ctatgccctg cccgtcgatg
     3781 tccctatcgt cttcttcggt tatcacgaca atatccgcat agtggccggc aataccgccc
     3841 tggattgccc gcttggcttc atcccgacgc ccagctgagc caaacatgac gataagcttt
     3901 tgaggcgctt ttacctgcga gcgcatatcg ctcagcaact tttcgaagga ggccggggtg
     3961 tgggcaaaat caacaatcac atgataatct tggccttcct cgaccttggt catacggccc
     4021 tcgacattac caagcgccgc gatcccctgc tcgatttgct tattagtcag acctacagca
     4081 ataccggtac tcacggcagc cagcgagtta taaacattaa accgcgcggg caaatgaaga
     4141 ttaatgtgca gtgtgctatt cccgcgcttc actgtatact cgctgccggt tggagtggct
     4201 gtgatattcg tagcctttac gctaccgcca tcaatgccgt acaggatgga gcttggcaca
     4261 tcggcggcaa atagatcagc actgggatct tcagcattga taatgccggt ttgaagtccg
     4321 gcccggttgt cggcggccag ctggaacatc cggcgtttgg cgtcgcgata gcgttcaaaa
     4381 gtcccatgat aatccaaatg ctcatggtta acattggtca tcacggccac actgaacggc
     4441 acgccccaca cacgattttg ggctagggcg tggctggttg tttccagaac gagccactca
     4501 atcccagccg cgcgcattgc ttttatgcgg ctcatcaaag gaaaggcttg catgctggtc
     4561 atgtgaactt tattgtaacg ctgccactgc ggcgtgccat aaccaattgt tgtcatgtag
     4621 ccagccttgt agccagcctg cttcatcatc gtccagataa gtgtggtggt ggtggtttta
     4681 ccgtcagtgc cagtgacgcc aataactttc aagccgcgcg ccggaaagcc atagtaaaga
     4741 ttggccaaaa acgtctccac cagatggcca tagggttcaa tgcggcgaaa aagccgacgc
     4801 gggataagag tttttacaat gcggcgaaac attagcttat attgtagctt tcttaaggtt
     4861 ggccgtcgac tagcgaataa tgccggtttg aatataaaag gcatagagat cgggatcggt
     4921 cgtagcaata gttggacata cgtggggcgg ccattcggca aattgatcgg gatggggcag
     4981 ctttcccatc attttcaacg acgacaaaag ggctggctgt tcgggcagtg ccgcagcttc
     5041 aacgcgatcc cccagaaggg tggccgttac tttgagcagc cccgccaatg atgcatagcc
     5101 cagcttctgt gcctgcagtt cagcggcctg tttgactgaa aatgaaagat gaataacaac
     5161 acgcatgtgc ttaagactag agcattggga ttaaattttt atatacattt atagtgcagg
     5221 cacaaattag gcacaattct gtgccatatc tatgtaggat ttgtcccttt tctgtgccaa
     5281 attaggcaca aacttgcaaa tgaatataag aaattttttc acatagccgg tttgacttaa
     5341 aagatcgatt ttgtcaggat gtaatcatag ccattattga gggggaatta tgaaattgaa
     5401 cgcactactg caagagccgc ataaaatcgg atgggcctta tttgcattat cagccagctt
     5461 cttactagct gttattgttg ccggttcgct aggaatgacc tcgctagcaa tgatgctcag
     5521 ccagattgct caaccaattt gcgcatccgc aattggggta cttgtctatc tggcaatggt
     5581 tgcgcgccgc catgaaaaaa cttcgctaga aggcagttaa tgttaataaa atagacggtg
     5641 gttgcggcga gactgatagg ccattgccaa ccgctcccct atttggttcc atagctgata
     5701 cttaccgtgg tcaacaacca gatcgtatgt accagcatac tcggtgattt cgggattaaa
     5761 ccctcgttta aagcgggcta aaccggccag agggtggttg gtattatcaa tttcggcagc
     5821 tggtggcacg ccgtgaaggt cgtactcttt aatcccttta gctttgagcc agcgcataat
     5881 ctcccactgc atcaaataag gagcttgcaa ctcggaatga tcccgatatg acgcgccatc
     5941 tttatacagt gccgattccc ccaaataggt tataaatgct ccggctactg gcttgccctc
     6001 ataggtggca aaaaacattt ggccggtcct ctgcttagaa tgcagatccc aaaaatcaaa
     6061 aaaatactgc ttgttgcgaa catatacacc ggctcgttca tatgttgttt ttgacatctc
     6121 atacagacgg tcaacgctct caagggtggt ttcaaccgtt tcaaccttca cacctttttt
     6181 gactgccagg cgaatattat agcgagtctt ttgcttaaat gaagctaaaa tgtcatcttc
     6241 acccggagta agatcaacga ccacggttgc aatattgtac tgaatatctc gagacgccct
     6301 taccagcccg gccgatgcga gcgcacttag tttggtggcc ttgggaagtt caggatcaat
     6361 ttttaccatg aatgcaccgg tctttttaag ttctgctgtt atccgcttaa gctgctgtat
     6421 gtcttgcacg ccgggcccct tggggatgta ccacagttca ccgaacattg gcacattccg
     6481 caccaaggcc agaatagcgg taccttcaaa catcaaatag ttggctttcc agccgtgacg
     6541 cgacttggtt tcgccgaaag cttttgcctg caacacgttg ccgccatcag gattggccgc
     6601 tatcaacgcg tcccactgcg caagctcatc agcggttgcc ggcttcaata gcattaccgg
     6661 ccccgcaccc tgcgatagag cgaataagcc gcgtatttaa ccggcttgac cgggatatcc
     6721 caggtgcctg cgtaatcagc ttgctgtccc ccaaaagcct ctttaaaccg gcttaaggag
     6781 gctagatgat gcgaactgtc accagccgaa gccactcccc ataaatcaaa ttgctttaac
     6841 ccctcgacct tcgcgtctac gatggcttgc cataccaacg aaaccgaggc cttcacccgg
     6901 cgattctctt cttgataggc gccagcatga gcgtaatagc gtataccact ccaatcgtag
     6961 aacaacgcag cggctaccgg cttgccttca acctcagcca ggtacagctt ggcggcatcg
     7021 tgcggctgga aaacctccca gagattctgg aagtattcgc tgggatgaaa cgtgacatga
     7081 gatcgttttg ctgtatcagc cagcattttt agcaacacat cgaagtcttc tgccttatcg
     7141 gtcttcctaa tgacgattcg ccgccgtttc gtgccattaa ctaggttgcg gtggcccgat
     7201 gctaggtcgg tacgaagctc agcctctgat ttggaaaggt cgataatccg agtatgctgc
     7261 ggatcgacct cggcaatgtt tcgagcccga agcgtaacca attcagtaat gcttatgctt
     7321 ctctgcggct caacgcgaac aaagtcaata cccagctcct tagccgcatg cgccgctgac
     7381 atgatcgccg tactcaatgc ggcctcatcg cgcgccgccg ggccataagc acagtgcaaa
     7441 tagcgcaacc catggctgcc gcgaatcgac gccgaccact gccacccctc gtccgcctcc
     7501 cacacaattt cacgccccaa gtgttgttga aataacatcc acaagcggct ctgcaaaatg
     7561 ctgccgccaa gcatttgcaa tgtgtggtcc cagtcgttgg gcggcagtcc gtgtttcata
     7621 ctgcgtattg tacgagcaat cctgatatac ttctacccta tgaatatatt gggtattgaa
     7681 accagttgcg acgaaaccgc tgcagcagtg gtaaaagacg gacgaattat cctcagcaat
     7741 gttgttgcct cccaaggcga ccttcatgcg cagtttggcg gcgtggtccc cgagattgcg
     7801 gcgcggagcc atattgaaat gatactgcca attgtcaaaa aagcccttga tgaagccgaa
     7861 actgactgga acgctattga tgcaattgcc gttaccagag gtccgggctt gattggttcg
     7921 ctgttaattg gaatcttggc cgcccaaacg ctagccacgc taaaaaatat gccgcttata
     7981 cccataaacc acatcgaggc tcatacatat gccaactttt tactccccgc ccccctatca
     8041 ttcccccttt tgtcgctcac ggtttccggc agccacaccc aactagtgct ctttacggat
     8101 cacggccagt atcgggtact tggaaaaacc ttagacgatg cggccggaga ggccttcgac
     8161 aaagtagccc ggatgattgg actttcccgc tccagcggcg cggccattga agtcgcagcc
     8221 gcgcacggaa ataatcaagc ttttgctttt cccaagccca aattaggtaa ggaaagtttt
     8281 aattttagct ttagcggact gaaaaccgcc gttttgcgca cagcccaagc cgcggtggga
     8341 cgcgatcatc gaaccttgtc acatgaatta ccggctttat tacgtgattc tcaacgcaat
     8401 gatctggcgg ccagctttca aaaaaccgtt gtcgaaatac tggccgggcg tctgctccaa
     8461 gcatacgagg cccactcccc ttcttcggtt gttattggag gcggcgtggc agccaatcag
     8521 gcgcttcgaa gcaaagttgc cacactgctg ccagttacag tcggctaccc cccgcttgat
     8581 ttgtgcaccg ataatgctgc catggttgca agcctgggat actttcagct tcaaagcggc
     8641 cga
//
LOCUS       DASVGB010000111         4217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_527200, whole genome shotgun sequence.
ACCESSION   DASVGB010000111 DASVGB010000000
VERSION     DASVGB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527200"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF272_03510"
     CDS             complement(<1..217)
                     /locus_tag="VF272_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259973.1"
                     /translation="MSKPVTLIAFDCWGTVFTNAQKPHPFAVFAKRLDKSLTDRTYVA
                     LFEQHLMTEIYPDLKLPIRRLLGELGIP"
     gene            complement(234..755)
                     /gene="msrA"
                     /locus_tag="VF272_03515"
     CDS             complement(234..755)
                     /gene="msrA"
                     /locus_tag="VF272_03515"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7259974.1"
                     /translation="MEIITLGGGCNWCIEAAFQLVKGVTGTVVGYAGGSVPHPSYEAV
                     YSGKTGHAEVAQVTFDPTVISLEDILDVFWIIHDPTTLNRQGPDVGTAYRSIILYNSD
                     EQKAIAERSKEVVAKLWPDPIVTEIVPFTVFYNAGAEHQNYYRNNQEARYCQIIINPK
                     LDRLRQKFAEKLA"
     gene            complement(755..1330)
                     /locus_tag="VF272_03520"
     CDS             complement(755..1330)
                     /locus_tag="VF272_03520"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7259975.1"
                     /translation="MKVVILHAIQSNPSAHWLPWLKKQLEEEHIEVVIPELSAPDHPD
                     RAQWLADAKAAIGSVDPDELILVGHSLGVTTALDYLEQAEMPVRGLVSTAGFYQDYGN
                     ELNSYFLKTKAIDMQVVRHHLTNAFVFYGDNDPYVTQEALRAMADGLLVDPVVIKGGG
                     HLNDEAGYTSFPQLFETVMELIDTPSLDFFA"
     gene            complement(1327..2838)
                     /locus_tag="VF272_03525"
     CDS             complement(1327..2838)
                     /locus_tag="VF272_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7259976.1"
                     /translation="MSKQQRLILVVSILASFVAFLDIAVVNVALPAIQRELGGGLAAQ
                     QWVVDAYLITLGSLILIAGSLSDLFGRKKILAAGLIGFAVASVLCAAAPTSEFLIVAR
                     ALQGVAGALLVPSSLALIISSFSGPAQGKAIGTWTAWTGISFIVGPLVGGLMVDLASW
                     RLVFAINVIPIAVTLFLMKSITLKEAVRENTKVDFLGSILCAAGLGGTVFALIEQPNY
                     SWTHPLIYLPLAAGIFLLALFFWHERRTPHPMLPLSLFKVRNFTAGNLATLAIYAGLT
                     VSSFLITVYVQQVGGYSALAAGMTLLPVTILLFMLSSRFGALAAKHGPRWFMTFGPII
                     GGLGFLLLLSVNRDLNYWTQLFPGIMLFSLGLAMTVAPLTTAVLADIDNRQAGIGSAI
                     NNAVARIAGLLAVAALGAVVATQFNTVVHHNVERSTLSQPAQEAVEKASVFDIKAAET
                     LGSERELAEDVLYNASLSAFHVGVWVMALLLIAGGLISAVGIRNRQPEKAQAP"
     gene            2873..3673
                     /locus_tag="VF272_03530"
     CDS             2873..3673
                     /locus_tag="VF272_03530"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7259977.1"
                     /translation="MLPPLIDAIILGIVEGLTEFLPVSSTGHLILVGHLLGFTGATAA
                     TFEVFIQLGAILAVVWIYRQRFLSLLSLAPSKSGVQGRRGIMLLVITTLPALIFGFLL
                     HDFIKTSLFTPLTVALALGVGGVVIILFEKFHTPKKAATLNTITVRQAAAIGFFQVLA
                     LWPGVSRAGATIIGGMVSGLDRKTAVEYSFLAAVPVMLAATGYDLLKSLPNLQPSDIP
                     LFAVGFVTSFIAALIAIKWLLKLVQTQTFTAFGWYRIVLALIVVLVLL"
     gene            3902..>4217
                     /locus_tag="VF272_03535"
     CDS             3902..>4217
                     /locus_tag="VF272_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7259978.1"
                     /translation="MREVIPPNAKLLPPNANRVFKGQIYDVYQWEQELFDGSTGTFEM
                     LKRPDTVKVLAVKDGKIVVLEEEQPYHGTFFDVPGGRHDDASETELQAMQRELREETG
                     MSF"
BASE COUNT         1091 a         1159 c          984 g          983 t
ORIGIN      
        1 tcgggatgcc aagttcgcca agcaaacggc gaataggcag ttttaaatca gggtatatct
       61 ccgtcatcaa atgttgctcg aacagcgcaa catacgtgcg gtcggttaag cttttgtcga
      121 ggcgcttggc aaaaaccgca aaaggatgcg gcttttgagc attggtaaaa accgttcccc
      181 agcagtcaaa agcaatcagc gtaacgggtt tgctcatccc cgtattatac tcttcaagcc
      241 aatttctccg cgaatttctg acgcaatctg tccagcttgg gattaatgat aatctggcag
      301 tagcgagctt cttggttgtt gcgataatag ttttgatgct ccgccccggc attatagaaa
      361 accgtaaacg ggacaatctc ggtgacaatc gggtccggcc agagcttagc aaccacctct
      421 ttcgaccgtt cggcgatcgc tttttgctcg tcagaattat agagaataat tgagcgatag
      481 gcggttccaa catcagggcc ttggcgattc agcgtagtgg ggtcgtgaat aatccaaaac
      541 acatccaata tgtcttcgag cgaaatgact gtagggtcga aggttacctg ggccacttca
      601 gcatgaccgg tcttcccgct ataaaccgcc tcataactag gatgaggaac cgaaccgcca
      661 gcataaccga ccacggtgcc agtaacccct ttcactaact gaaaggcagc ttcaatgcac
      721 cagttgcaac cgccgcccag cgttatgatc tccactaagc gaagaagtcc aggctgggcg
      781 tatcaataag ctccataacg gtttcgaata actgcggaaa actggtgtag ccggcttcgt
      841 cgtttaaatg tcctccgccc ttgatcacca ccggatccac caacaaaccg tcggccattg
      901 cccgcagcgc ctcctgcgta acatacgggt cattatcgcc ataaaacaca aacgcattgg
      961 taagatgatg ccgcaccact tgcatatcga tcgctttggt ttttaaaaaa tagctgttca
     1021 actcattgcc ataatcttga taaaatccgg cagtagacac gagtccgcgt accggcattt
     1081 cagcttgctc cagataatcg agtgccgtgg taacacccag cgaatggccg acaagaatca
     1141 actcgtcggg atcaacacta ccaattgcgg ccttggcgtc agccagccac tgtgcccggt
     1201 ccggatgatc gggagcgcta agctctggta tcacaacctc aatatgttct tcctcgagct
     1261 gttttttcag ccacggaagc cagtgagccg aagggttgct ttggatggcg tgcaaaataa
     1321 caactttcat ggagcttgcg ccttttccgg ttgccgattg cgtataccaa ccgcggaaat
     1381 caacccgccg gcaattagca gaagtgccat aacccagacg ccaacatgaa atgccgacaa
     1441 tgaggcgtta tacaaaacgt cttccgccag ctcgcgctcc gatcctagcg tttctgcagc
     1501 tttaatatcg aataccgatg ccttctccac cgcttcctgg gccggttggc tgagcgtact
     1561 gcgttctaca ttgtgatgta ccaccgtatt aaactgagtt gcgactacgg ctcccagggc
     1621 tgccaccgcc agcagtccgg caatccgggc gacggcgtta ttaattgccg aaccaatccc
     1681 agcctgtcga ttgtcaatat cggccagaac cgcggtagtc agaggcgcca cggtcattgc
     1741 caaacccaaa ctgaagagca taataccagg aaacagttgt gtccagtaat taagatcgcg
     1801 attgacactc agtaataata gaaatcccag cccgccgata atcggcccaa aggtcataaa
     1861 ccatcgcggg ccatgcttag cggctaaggc gccaaatcgt gaagacagca taaacaatag
     1921 gatagttacc ggaagcaatg tcatcccggc agccagcgcc gaatagccgc caacttgctg
     1981 cacgtaaacg gtaattaaaa atgaagaaac cgtcagtccg gcgtaaattg ccagggtagc
     2041 caggttgcca gcagtaaagt tgcgaacctt aaagagcgat aacggcaaca tcggatgcgg
     2101 cgtgcggcgc tcatgccaga agaataacgc cagaagaaaa attccagcgg ctaggggaag
     2161 gtaaatcagc ggatgcgtcc aactataatt tggttgttca attaaggcaa aaaccgtacc
     2221 gcccaaaccg gcggcacaaa ggatactgcc aagaaaatct acctttgtgt tttcgcggac
     2281 agcttcttta agggtaatgc tcttcatcag gaacaaggta actgcaatcg ggatcacatt
     2341 aattgcaaat accagccgcc acgacgccag gtcaaccatc aacccgccta ccagcggccc
     2401 aacaataaat gatattccgg tccacgccgt ccaggttccg attgctttgc cttgggccgg
     2461 accagaaaaa ctggaaataa tcagcgccag tgaactcggt accagcaagg ctccggcaac
     2521 tccttgcaga gcacgggcca caatcaaaaa ctcgctggta ggcgccgcgg cacacaatac
     2581 cgaggctacc gcaaaaccga tcagcccggc cgccagaatt ttttttcgtc caaatagatc
     2641 ggaaagcgac ccggcaatca ggatgagcga tccaagcgta atcaaatagg catcaaccac
     2701 ccattgctgg gcggccagtc ctcccccaag ctcgcgctgg atcgccggca acgctacatt
     2761 taccacggca atatcaagaa aagcgacgaa tgaggccaga atgcttacta ccaatatgag
     2821 gcgttgttgc ttgctcatac cgccatcata gcaaatggta taagctagtt gtatgcttcc
     2881 accactgatt gatgccatca ttttgggaat tgttgaagga ttaaccgaat tcctgccggt
     2941 ttcatccacc ggccatttga ttttggtcgg acatttgctg ggttttaccg gagccaccgc
     3001 cgctactttc gaagttttta ttcagttagg cgcaattctg gctgtcgtgt ggatttaccg
     3061 ccagcgtttt ctctccctgt taagcttagc gccttccaaa tccggcgttc aaggccgccg
     3121 cgggatcatg ctgctagtca tcactacgct gccagcgctt attttcggat ttctcctcca
     3181 cgacttcatt aaaacttcat tattcacccc gcttaccgtg gcactggccc tcggcgttgg
     3241 cggcgtcgtc attattctct tcgaaaagtt tcacacgccc aaaaaggccg ccacactcaa
     3301 caccattacc gttcgccaag ccgccgccat cggtttcttt caggtcctgg ctctctggcc
     3361 cggcgtttct cgggcaggcg caaccatcat cggcgggatg gttagcgggc tcgatcgcaa
     3421 aaccgctgtg gagtattcgt ttttagcagc cgtcccagtg atgttggcgg ccaccggata
     3481 tgatttgctc aaaagcctgc ccaacttaca gccaagcgat ataccactat tcgccgttgg
     3541 ttttgtcacc tcatttatcg ctgccttaat cgccattaag tggctgctta aactggtaca
     3601 aacccaaacc ttcacagcct tcggctggta tcgcattgtt ctggcgctca ttgttgtatt
     3661 agttttgctc taaatcaagt cccaatccaa aaagaactct atccttcccg agggccagcc
     3721 tattccggcc ctgcattggt gcggccagcg caccaataaa tcagctcaaa ctttctcaat
     3781 tttttcgcta ctcaaatgtg agtacctagg tactcaattt atacacgaat gtaagtactt
     3841 actgagtaca ttgatactca tttttgagta gctaccaaaa aacgtatgct atcctgagaa
     3901 aatgcgcgaa gtcatccccc ccaatgctaa attgctcccc cctaatgcta atcgcgtttt
     3961 taaggggcaa atttacgacg tctaccaatg ggagcaggaa ctattcgacg gctcaaccgg
     4021 cacttttgag atgctcaagc gacccgatac ggttaaggtt ttggcggtga aggatgggaa
     4081 aattgtcgtg ctcgaagaag agcagccata tcacggaacg ttttttgatg ttccaggcgg
     4141 ccgccatgac gacgcgtcgg agaccgaact tcaggccatg caacgtgaac tgcgcgaaga
     4201 aaccggcatg tcattta
//
LOCUS       DASVGB010000112         3992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_786772, whole genome shotgun sequence.
ACCESSION   DASVGB010000112 DASVGB010000000
VERSION     DASVGB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786772"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VF272_03540"
     CDS             <1..1043
                     /locus_tag="VF272_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259979.1"
                     /translation="GPRDRASSFATGAWLASHRPTSLVETRVNPGDTGTFEFPITAPA
                     TSGSFFERFNIVAEGDRWMNDIGLGYAIKVVQPSYTWALSGQAAYVDSSKTVPVDLNN
                     LSPGQTAWFVLRATNTGNVSWTNTGAHPVRLGTEGPKDRPSRYRVGSWLAGHRPSALI
                     ESSVSPGQVGSFEFPIEVPPGGGVFLERFNLVAEGFTWMNDMGFNYHTRVNGSYAWAP
                     VNQYAYTDNTKTISVDLSNLSPGQTVFIGFTARNTGNAVWYQGGAYPVRTGTSRPLER
                     ISAFAAPSWLWNRRPSGLVETSISPGQLGTFEFEYTAPLIAGTYHEYFQPLAEHITWM
                     NDPGLHFYTVVQ"
     gene            complement(1050..2084)
                     /locus_tag="VF272_03545"
     CDS             complement(1050..2084)
                     /locus_tag="VF272_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259980.1"
                     /translation="MKLLHKRILFVLLLVAASFLSYPIHYPRFVHPEAPTPEAASSSS
                     QSTRPYTIIVMPDTQAYAQFFPEIFMAQTSWIAAQAKERNIVFVSQEGDIVNIPEAEG
                     QWQVASAAMALLDGIVPYGLAPGNHDFTHTGSAPLYQKYFPMSRLKRSPAWGGSFLEF
                     NTQLNHPNLHRYDKNSYQLFEVGNTKYIAFNLEFCPPDDVVYWTGWMLALYPNRQAII
                     TTHSFTNFKGGRSNSQICKHYGAEGLNAGEEIWQKLAVDRTHLNISLLLSGHDIITTR
                     GAARRTDVVDNRPIHQLLANYQQLPNGGNGYLRIMTFKPAEQKIQVETYSPYIDRYMT
                     DPENQFDLPL"
     gene            2179..3153
                     /locus_tag="VF272_03550"
     CDS             2179..3153
                     /locus_tag="VF272_03550"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX7259981.1"
                     /translation="MLKMPKTLQQFLLEELKDHPERIDLVMVMSNLATIGKLLSSQTN
                     RAGLANIRGLSDTENVHDETQAKLDLYANDLCKSYLESTGSFAALASEEEEQVIDLHS
                     PDAKYIIAFDPLDGSSNIDVNVSVGTIFSVHAKRDDVPAGDEQQFFQLGKDQALAGYI
                     LYGSSTILVFSWGEGVHEFTLDVDLGEFLLSDAHMKIPEACTYYSVNESYVASMAEKD
                     QKYVAVLREKTTKQRWIGSFVADFHRNLIKGGVFFSPPVDTSGQQQFKPKLRLNYELK
                     PLAFLVEQAGGSAMAGDERILEIMPHELHQRASATLGNVEIVTLYKES"
     gene            3154..>3992
                     /locus_tag="VF272_03555"
     CDS             3154..>3992
                     /locus_tag="VF272_03555"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX7259982.1"
                     /translation="MNHAEQLKKTAQAMMAEGKGLLAADESTATIAKRFESVGMENSE
                     EHRRQYRQLLLEAKGAEAFISGVILYDETARQKDDAGKPFVQSLSDQGIVPGIKVDIG
                     PRELALHPGEKVTEGLDGLGERFAEYAGMGIGFAKWRAVIIIDGTELPSQACLEANAH
                     ALARYAALAQEAGIVPIVEPEVLMDGAHSLERCLEVTANALDVLFAQLAAQGVMLEGT
                     ILKPSMVIAGSGSDTASDRQTVAEQTVACLKQHVPENLAGVMFLSGGQSDEEATAHLD
                     AMN"
BASE COUNT          965 a          920 c         1107 g         1000 t
ORIGIN      
        1 aagggcctcg tgaccgcgcg agcagttttg caaccggagc gtggctggct tcgcatcgtc
       61 ccactagtct cgttgaaact agggtgaatc caggtgatac gggaacgttt gagtttccaa
      121 ttacggctcc ggcaacaagc ggctcgttct ttgagcgttt caacatagta gccgaggggg
      181 atcgatggat gaatgatatt ggcctgggct acgcgatcaa agtagtgcaa ccgtcttata
      241 cctgggcact ttcagggcaa gcagcatatg ttgatagctc aaaaaccgta cctgtagatt
      301 taaacaacct ttctccgggg caaaccgcct ggttcgttct gcgagctacg aatactggca
      361 atgtttcttg gaccaacact ggcgcccatc ctgtccgctt aggcactgaa gggccaaaag
      421 atcgccctag ccgttatcgt gttggctcct ggcttgctgg tcaccgccct tctgccctca
      481 ttgaatcctc ggtaagtccc ggtcaagttg gcagctttga gtttcccatt gaggtacctc
      541 cgggaggggg agtttttctt gagcgcttca atctagtagc tgaaggcttc acttggatga
      601 atgacatggg tttcaactat cacaccagag ttaacggcag ttatgcatgg gctccggtta
      661 accagtacgc ttataccgac aacacgaaaa ctatctccgt tgatctttct aatcttagtc
      721 cgggtcagac ggtttttatt ggttttacag cacgtaatac cgggaatgcg gtgtggtatc
      781 aagggggagc gtatccggtt cggacgggta catctcggcc attggaacgg attagtgctt
      841 ttgcggcacc ttcgtggctg tggaatagga ggccatcagg acttgtggaa accagtatat
      901 cgcccggcca actcggcacc tttgaatttg aatatacagc tccgctgatt gccgggacgt
      961 atcatgagta ttttcaacct ttggccgaac acattacctg gatgaatgat ccgggcttgc
     1021 atttctatac cgtggtgcag tagtccggac taaaggggca agtcgaattg gttttcggga
     1081 tcggtcatat agcggtcaat atatggtgag taggtttcta cctgaatttt ttgttcagcc
     1141 ggcttaaatg tcataatgcg caaatagccg ttgccgccgt taggcagttg ttgatagttt
     1201 gcgaggagct gatgaatcgg gcggttatcc accacatcgg tacgacgggc agcaccgcgg
     1261 gtggtaatga tatcgtgccc ggatagcaac aatgagatat tgaggtgcgt gcggtcaacc
     1321 gctagcttct gccagatttc ttctccggcg tttaacccct cagcgccgta gtgcttgcag
     1381 atttgactgt ttgaccgacc gcctttaaag ttggtaaacg aatgggtggt aataatggct
     1441 tggcggttgg ggtacagcgc gagcatccaa cctgtccaat agacaacatc gtccggtggg
     1501 caaaattcta gattaaaggc tatgtacttg gtgtttccta cttcaaataa ctggtagctg
     1561 tttttatcgt accgatgcaa attgggatgg ttcagttggg tattaaactc caggaaacta
     1621 ccgccccaag ccggcgagcg cttcagtcgc gacattggga aatatttttg atacagcggc
     1681 gcgctgcctg tgtgcgtgaa atcatgattt cccggcgcca gaccataggg gacaatgccg
     1741 tctaaaagtg ccatggcggc gctggctacc tgccactggc cttcggcttc cggtatgttc
     1801 acaatgtcgc cttcttgcga aacaaagaca atatttctct ccttagcttg ggcggcaatc
     1861 catgaggttt gagccataaa aatttccgga aagaattggg cataggcctg agtatctggc
     1921 ataacgataa tggtatatgg ccgggttgat tggctggaag atgaagcagc ctctggtgtt
     1981 ggtgcttccg ggtgaacgaa ccgcgggtag tgtatcgggt acgataaaaa cgaggcggcc
     2041 accaaaagaa gaacgaatag gatacgtttg tgcaggagtt tcatgtcggt aagattaatc
     2101 caatgctact atagccaatc ggtactgcca acgattatcg gcgaccgtgc ggcgaatatg
     2161 cattataatc aaggctagat gctaaaaatg ccaaaaacat tgcaacagtt ccttcttgag
     2221 gaactgaagg atcatccgga gcgaatcgac ctggtaatgg tgatgtctaa tcttgccact
     2281 attggcaagc tgctttcctc ccaaaccaac cgggccgggc ttgccaacat ccgcggtctc
     2341 tcggataccg agaacgtaca tgatgaaacc caggccaagc tggacttata tgcaaatgac
     2401 ctttgtaaaa gctacttgga aagtaccggt tcatttgcag ccttggccag tgaggaggaa
     2461 gagcaggtaa ttgatcttca tagcccggat gccaaataca ttattgcgtt tgacccttta
     2521 gacggcagca gcaatattga tgttaacgtg agtgtaggca cgattttttc ggttcatgcc
     2581 aagcgcgatg acgtaccggc cggcgatgaa cagcaattct tccagctcgg gaaagaccag
     2641 gcactagccg gctatatttt gtatggctcc agtactatcc tggtattcag ttggggtgaa
     2701 ggcgtgcacg agtttaccct tgatgttgac cttggtgagt ttctattaag cgatgctcat
     2761 atgaaaattc cggaagcctg tacctattac agcgtcaacg aatcgtatgt agcgtctatg
     2821 gccgagaagg accagaagta cgtggcggtg ttgcgcgaaa agaccacgaa gcagcgctgg
     2881 attggttcgt ttgtcgccga ctttcatcgc aatcttataa aaggcggcgt cttcttttcc
     2941 ccaccggtcg atacaagcgg gcagcagcag tttaagccga agctgcgttt gaattatgag
     3001 ctgaagcctc tcgcattttt ggttgaacag gcaggcggtt cggctatggc tggcgacgag
     3061 cggatcttgg aaatcatgcc gcatgagctc caccagcggg catctgccac tctgggaaat
     3121 gtcgaaattg taacacttta taaggagtca taaatgaatc acgcagaaca gttaaaaaaa
     3181 accgcccaag caatgatggc cgaaggtaaa ggcttgctag ccgccgatga aagcactgcc
     3241 accattgcta aacggtttga atcggtgggg atggaaaaca gcgaagagca tcgccggcag
     3301 taccgtcagc tactgttgga agcaaagggg gctgaggctt ttattagcgg ggtgatttta
     3361 tatgatgaga ccgcccggca gaaggatgat gcgggtaaac catttgtcca aagcctgagt
     3421 gatcaaggta ttgtaccggg catcaaggtt gatattggtc ctcgcgaact ggctcttcac
     3481 cccggcgaga aagttactga aggacttgac gggctgggtg agcgctttgc cgagtatgct
     3541 ggcatgggta ttggttttgc gaagtggcgg gcggtaatca ttattgatgg cactgagctg
     3601 ccgagccagg cttgcttgga agcaaatgcc cacgcgttgg cccgttatgc tgccctggct
     3661 caagaagcgg ggattgtgcc aatcgtagaa ccagaggtgc tgatggatgg tgcgcattct
     3721 cttgagcgct gcttggaagt aaccgctaat gcacttgatg ttctgttcgc gcaattagca
     3781 gcccaaggcg taatgctgga aggcaccatt ttaaagccga gtatggtgat tgccggcagt
     3841 ggctctgata cagcatccga tagacaaacc gtggccgagc aaaccgtggc ctgcttgaag
     3901 cagcatgtac ccgaaaacct agctggcgta atgtttcttt ccggcgggca gagcgacgaa
     3961 gaggcgaccg ctcacctgga tgccatgaat aa
//
LOCUS       DASVGB010000113         7513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1095417, whole genome shotgun sequence.
ACCESSION   DASVGB010000113 DASVGB010000000
VERSION     DASVGB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095417"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VF272_03560"
     CDS             <1..1131
                     /locus_tag="VF272_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259983.1"
                     /translation="DLTTTSTTAALFNAAATTLNIGGVATAITVGATTGITNVRNELQ
                     VGSGTTDATQSLLQVDSFDTYADTATCSTTVNQGAIYYNTKSNAVRGCVNGVWEDMVS
                     TASLGLQLFGVVPDSGPTPGNWVGINATIANNGPCKVYVGSVNSGSSASIRWTSCTAF
                     SGGRKVIVPAQTSDVAITTTNGNFVHVCLTGTDGQPAISTSGTENANLPAFSAAAPIL
                     CLADVRTSATAINAIYDTRTFTTTTKEIVNNTSATPGVGMLVIQSGTAGQVNTSSTTA
                     GSAKLRGVIVAATSGAQTANTMNAIIATAGPNYAKATAATVGAYVQNGATAGYANTAA
                     NAPAPLNGAYASLGLALSGFSTTCGVLADTCRTSIAVNLNPR"
     gene            1140..1661
                     /locus_tag="VF272_03565"
     CDS             1140..1661
                     /locus_tag="VF272_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259984.1"
                     /translation="MKATNLLYAAGGGLVVLFITTYVLVLNSPASIEVPKPIKSQLNF
                     DPILPSQKSFEIGSITYTLDASSFRYDKEAGILTYLLLVNQRKLTITQQAYPEILVYD
                     KLVGAMHQYGEVPVKLGRVALTRPENLKGKQTAVLSTFNGSSGVLIFISPETDLAKDQ
                     WRQLFNNLRVHNE"
     gene            1654..3858
                     /locus_tag="VF272_03570"
     CDS             1654..3858
                     /locus_tag="VF272_03570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7259985.1"
                     /translation="MNRRQASHAFAWLFIGAIVLSSFSSTSVRASIGSCVANITPHQV
                     LVNSQTDFVIQLDNTDANTIHWIQIPQLAGYYSVESASAGGWSVGIDADAITFTGGSL
                     APGESAEIHLVATAAPDPRTVYWNVQVSDGSGGGVGRFCDGDTSVESTQTEPPQLSGI
                     TIANVGAHTITLNWQTNQPATSQVRYGTTAGYGSQSPVGSTLRTSHQVTLSGLMTNTA
                     YHYQVISGNSNGGSVSEDSTFLTAIEEKTGGGSIPDKNDSATSVSVPVKAVPTEHVPP
                     TVSLSTNLTKPFAKAPTMSGMAADNEAVARVEYSTDGGKNWQLVDSALGISGKKVSFS
                     FTPSGLEDGNYGLLVQAVDTSNNKAATAPQTLIIDRLPPEVGGTVNSIGAQIVSPGIS
                     GIIYGLVGVDQKVTLSAVGGATQIQLRAKRGGQPGGVFTLTHSQDNGLWSGIISFTHP
                     GSYTLVAEALDGAGNRTQRDLQTLLILPSTKLVDQATGKPVARAKLTVFYLEPESNTW
                     TVWDSAAYGQQNPQRADVGGNVNFLLPSGTYYLQAEAPRYRTVMTNIFSLNESTPLTA
                     DITFKKLPFGVPMWFPTVQKFKTSSHAKTQSRNQLVADAEAPNFNLRTTESASVSLLS
                     YAGKPTVLSFVSTWSPPAKEQLGVLAATQSNPDINVVAVNIQENGQKVKASLQIAGYT
                     MPMIVDQDGTMVEPYRLQSLPTHYFLDRHGAVKKVMVGVLSKEELLDGLGGL"
     gene            3878..5500
                     /locus_tag="VF272_03575"
     CDS             3878..5500
                     /locus_tag="VF272_03575"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7259986.1"
                     /translation="MLPLQFFGQNFHFAVNLCAALVFFAVCWLYLDAWSNRRGWKEFY
                     KWAGFALLAVASLVHAAEIEQSALGQSILGDASDNVGVILKIAGYIMIIVGLAMDPLQ
                     PVPKNKGLGLSTEKEKTPPNTKPAPALLVFPVASKVLLAVGSLGIAFLYFRRATTGLE
                     RHLRPVAIAFLLIGVADLLHLLSFWRMSDNPLIFNAVAAFGWVWFLEHTIFLIGVVIL
                     GYWVWNYLTKRFQSQLFMIFTSAVAIIFLITTVSFTYLILGSIQKQTLANLTTASNVL
                     KYALQSKQAETQAYTETLSQNPAIIAAVQSGDHNQLVQQTATFLAVKNQSNLLITSDR
                     GKVLLRADDPSRWGDSYSADPLVRRALIGRVNSSIQSKEGVVAPQLYIKTASPIRNSA
                     NEIIGVAIVGLLIDNGFVDGIKSSTGLDSSVYSGNVRAATTLLAADRKSRLIGVKEVN
                     QDVQARVLKEGKSYAGVTTLSNRPFLAVYAPLKDIDNVTLGMLQVSQPQDSILQAAGR
                     SIELTFLFAVVLMIVATVPAYVISNYLTEQLK"
     gene            5510..>7513
                     /locus_tag="VF272_03580"
     CDS             5510..>7513
                     /locus_tag="VF272_03580"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX7259987.1"
                     /translation="MVSQRFIRSIVYVSVSMLAVLAGIFMVSTLTKTWDVLHFNPFAL
                     LSMAAIISNVVLLVLINRQKRKAPETFWFSLFLLEIIYWATCEMMQRLSSTPEAAISW
                     YSISSPGWVLLGVTYWIYTLQVTDNMRFIRTLPGILAVIAAPLSFLLLALKTPWLINY
                     DIQSVIANPWGFYSAYGPLFNYFLIWLEVFFIASLIMMFRRYRSTEDPRVKRQALTTS
                     IALAIPLAVGSFTDGIFPAFDINVIPLAVTLTSIQAAITGYAIIKYHIFTFNTKMVAS
                     NILAGMNEVVLILNRNHRIEYINERTSNILGYPMSTLLGAKIETLFTDDRQMKDFKNI
                     ALSPSADTKFQNTNVDIKTVTGGHKSMLASSAQVVDPDTGEPAYILVMTDLSQVQRVV
                     LENKNLADTKLAMINALEDARLLEEELKNEKANVEKKVVERTHQLAEEQARLNASINS
                     LQVGFMIINGQKKLQSINAASYAILETKPSKSFDFAEVASRLSTTIDLESQIQQVVIT
                     KKPVTLKDLMWRGKYLRIFISPVITEKKEVIGTVILLQDITEEKILQRSREEFFSIAS
                     HELRTPLTAIRGNTSMLQGYFGEILEKNPDMKEMINDIHGSSVRLITIVNDFLDATRL
                     EQGGMKFTDVDVDTGKLAAIVIQDLQSVAQEKNIYIKNEIGSRM"
BASE COUNT         1923 a         1792 c         1860 g         1938 t
ORIGIN      
        1 gatctaacca caactagtac cactgctgcc ctatttaacg cagctgctac cactttaaat
       61 attggtggtg tcgctaccgc aataacagtg ggtgcgacta ctggcataac aaatgtgagg
      121 aacgagcttc aagtcggctc cggaacaacc gatgctaccc agtccttact gcaagttgat
      181 tcgttcgaca cttatgccga taccgccacc tgcagtacta ccgtaaacca aggtgctatc
      241 tactacaaca ccaaatccaa tgccgttcgt ggttgcgtga atggggtgtg ggaagacatg
      301 gtgtctaccg cctcgctagg actgcagtta tttggcgttg tgccggattc tggccccacg
      361 ccgggcaact gggttggtat taacgcgacc attgctaata acggcccgtg taaagtatat
      421 gtcggctccg ttaactcagg cagtagtgcc agcatccgct ggacgagttg cacagctttt
      481 tccggcggac gtaaagttat tgtgcccgcc caaaccagtg acgtggcgat tacgaccaca
      541 aatggtaact ttgttcacgt atgcttaact ggtacggatg ggcagccggc aatatcgaca
      601 tcggggactg aaaacgccaa cctaccggcg ttttcagcag cagcaccaat tttgtgccta
      661 gcggatgtga ggaccagcgc gacggcgatt aatgccattt atgataccag aacgtttacc
      721 actacgacca aagaaatcgt caataatacc agtgcaacac ctggcgtggg aatgctcgtt
      781 attcaaagcg gtactgctgg ccaagtcaat accagtagta caaccgctgg ctcagcaaaa
      841 ctccgcgggg ttattgtggc ggcaacctcg ggtgcgcaaa ctgccaatac catgaatgca
      901 ataattgcca cggccggccc gaactatgcc aaagccacgg ccgcgacggt gggagcatat
      961 gttcagaatg gtgccactgc cgggtacgct aatactgctg caaacgctcc ggcgccgctt
     1021 aacggtgctt atgcatccct ggggctggca ctatcagggt tttctactac ctgcggcgtc
     1081 cttgcagata cttgccggac ttcgatagca gttaacttga atcctaggta gacagaagga
     1141 tgaaagctac aaacctatta tatgcggctg gcggcggact ggttgtgttg tttattacaa
     1201 cttacgtgct tgtactgaat agccctgcat ctattgaggt gccaaaaccg ataaaaagcc
     1261 agttgaattt cgacccgatt ctcccttccc aaaagagttt cgagataggg tctatcacct
     1321 atacgcttga tgcgtcgtca tttcgctatg acaaagaggc cggaatacta acctatcttt
     1381 tattggtcaa tcaaagaaaa ctaaccatta cccagcaggc ttatccggaa attttggtgt
     1441 acgacaagct ggtgggggcg atgcatcagt atggcgaggt tccagtcaag ttgggcaggg
     1501 tggccttgac ccggccggag aatttgaaag gcaagcaaac tgcagtgctt agtacgttca
     1561 atggcagcag cggagttctt atttttatta gtccggaaac cgatttagcc aaagaccagt
     1621 ggcggcaact gtttaataat cttcgggtgc acaatgaata gaagacaggc tagccatgct
     1681 ttcgcctggc tatttattgg ggcaattgtc cttagtagtt ttagtagtac gtccgtacgg
     1741 gccagcattg gctcatgcgt tgcaaacatt acgcctcacc aagtgctggt gaattcgcaa
     1801 accgattttg taattcagct ggataatacc gatgcgaaca ctatccattg gatccagata
     1861 ccccaactgg ctggctatta ttcagttgag tcagcatcgg ccgggggatg gtcggttgga
     1921 attgatgctg acgctattac atttacggga ggcagcctgg cgccgggtga atcggcggaa
     1981 attcatttgg ttgctacagc agccccggat ccgcggactg tttattggaa cgtgcaggtt
     2041 tccgatggat ctggtggagg cgtcggtcgt ttttgcgacg gtgatacctc ggttgaaagc
     2101 acccagaccg aaccgccgca actaagcggc atcacgattg ctaatgttgg cgctcatacc
     2161 ataacgctca attggcagac caaccagccg gctaccagtc aggtgcgcta tggaaccacc
     2221 gctggttatg gatcgcagag cccagtgggt tctaccctgc gcaccagcca tcaagttact
     2281 ctttccggtc tgatgacaaa tactgcctat cactatcagg tcatcagcgg aaacagtaat
     2341 ggcggaagcg tatccgagga cagcacattc ttaacggcaa ttgaggagaa aactggcggg
     2401 ggaagcatcc ctgataaaaa tgattcagcc acaagcgtga gtgtgccagt taaggcggta
     2461 ccgacggagc atgtgccgcc caccgttagt ctctccacta atttaactaa gccctttgct
     2521 aaagctccca ctatgagcgg gatggcagcc gataatgagg ccgtggcacg cgtggaatat
     2581 tcaacggatg gcgggaagaa ctggcaattg gttgatagtg cgctgggaat tagtggaaaa
     2641 aaagtttctt ttagctttac gccaagcggt cttgaggacg gtaattacgg tttgttggtc
     2701 caggcggtgg acacgtcaaa caataaagca gctacggctc cacaaaccct gattattgat
     2761 cgcttaccgc ctgaggtagg gggcaccgtt aactctattg gagcgcaaat cgtttccccc
     2821 ggtataagtg gcatcatata tggcttggtg ggcgtcgatc aaaaggtaac cttgagtgcc
     2881 gtcggtggag cgacgcaaat tcagttgcgt gccaagcgtg gcggccagcc gggaggcgtc
     2941 tttaccttga cccattctca agacaatggt ctatggtccg gcatcatcag ctttactcac
     3001 cccggaagct atacgctagt ggccgaagcg cttgatgggg ctggtaatcg aacgcagcgg
     3061 gatttgcaaa ctttgctcat cttgccttct acgaaattgg ttgatcaagc tactggtaaa
     3121 ccggtggctc gcgcgaagct gacggttttc tatcttgaac cggaatcgaa tacttggacc
     3181 gtttgggatt cggccgccta tggtcagcaa aatccccagc gagcagatgt cggtggcaat
     3241 gttaattttt tgctaccgtc cggaacctat tatctccagg ctgaagcacc gcgctatcga
     3301 acggttatga ccaacatatt ttctttaaac gagtcaaccc ccctaaccgc tgacattacc
     3361 ttcaaaaaat tgccattcgg cgtgcccatg tggtttccga ctgtacagaa atttaagaca
     3421 tcttctcatg ctaaaactca atcccgcaac caattagttg cggacgcaga ggcaccgaac
     3481 ttcaaccttc ggacaaccga gagtgctagt gttagcttac tgtcctatgc cgggaagccg
     3541 acagtcctta gctttgtatc gacttggtcg cctcccgcca aagaacagtt gggagttttg
     3601 gcggccacac aatctaatcc tgatattaat gtcgtggcgg ttaatatcca ggaaaacggt
     3661 caaaaagtta aggcgtcgtt acagattgcc ggttatacca tgcccatgat tgttgatcag
     3721 gatggtacta tggtggagcc ctatcgtctc caatcgctgc cgactcatta ctttcttgat
     3781 cggcatgggg ctgtcaaaaa ggttatggtt ggagtattat caaaagaaga actattagat
     3841 ggtcttggcg gattgtgatg ctgaggtgag gtaaagaatg ctgccactcc aatttttcgg
     3901 tcaaaacttt catttcgcag ttaatttatg cgcggctctg gtattctttg cggtatgctg
     3961 gctttacctt gatgcctgga gtaatcgccg ggggtggaaa gagttttata aatgggctgg
     4021 atttgccttg ctggcggtcg cctcactcgt tcatgctgcc gaaattgagc aatctgcatt
     4081 aggccaatcg atattaggtg atgccagcga caacgtgggg gtgatcttaa aaatcgccgg
     4141 ctatatcatg attattgttg gattagcaat ggatcctctg caaccggtgc caaagaataa
     4201 ggggctgggg ctatcaaccg aaaaagaaaa aacgccgcca aataccaagc ccgcaccggc
     4261 actattggtc tttccggtag cttcgaaagt cctgcttgca gtgggcagtt tgggcattgc
     4321 cttcctttac ttccgtcggg caactaccgg cttagagcgg catttgcgcc cggttgcgat
     4381 tgccttttta ttgatcggag tggccgatct tctgcattta ttatctttct ggcgaatgag
     4441 cgataacccg ctcattttca acgccgtggc cgcgttcggt tgggtgtggt tcttggagca
     4501 tacaatcttt ttgatcggag ttgttatttt aggctattgg gtatggaact atcttaccaa
     4561 gcgcttccaa agtcagctat ttatgatttt tacctccgcc gttgcaatca ttttcttgat
     4621 tactacggtt agttttacct atttaatttt gggtagcatt caaaagcaaa ccctggctaa
     4681 tttaactact gccagcaacg ttctgaaata cgccttgcaa agtaagcaag cggaaactca
     4741 ggcatatacg gaaacattaa gccaaaaccc ggcaataatt gccgccgttc aaagtggcga
     4801 tcacaatcaa ctcgttcaac agacggctac tttcttagcg gtgaagaacc agtccaacct
     4861 gctcattacc agcgataggg gcaaggtgct cttgcgagcc gacgatccat caagatgggg
     4921 tgactcctat tcagccgacc cattagttcg tcgagccttg attggcaggg tcaactcctc
     4981 cattcagtca aaggaaggcg tagtggcacc ccaactatat attaaaacag cgagtcctat
     5041 ccggaattca gccaacgaaa tcataggtgt tgctatcgtc ggattgttga tagacaacgg
     5101 cttcgttgac ggcataaaaa gttcgaccgg gttggacagc tcggtctata gtggcaacgt
     5161 ccgggccgct accactttat tggctgctga tcgcaagtcg cgcctgatcg gggtaaaaga
     5221 agtaaaccag gatgtgcaag ccagggtttt gaaggaaggt aaaagctacg ccggcgtaac
     5281 cactctttcc aaccgcccat tcctggccgt ttacgcgccg ttaaaggaca tagacaatgt
     5341 aacgctcggc atgctgcaag ttagccagcc acaagacagt attttgcagg ccgccggacg
     5401 ttcaattgaa ctaacgtttc tgtttgcggt tgtcttgatg attgtggcta ctgttccggc
     5461 gtatgtaatt tcgaactatt tgaccgagca actgaaataa tagctacgta tggtttcaca
     5521 gcggtttatt agaagtattg tttacgttag tgtaagtatg ctggcagtgc ttgctggcat
     5581 cttcatggtt tcgacgctca cgaaaacctg ggatgttttg cacttcaatc cttttgcgtt
     5641 gttatccatg gcggccatca tttctaacgt tgtcctattg gtactgatta atcggcagaa
     5701 gcgtaaagcg ccggaaacat tttggttctc cctgtttttg ctagaaatta tttattgggc
     5761 gacctgtgag atgatgcagc gactaagctc gacaccggaa gccgcaatct cctggtatag
     5821 tatctcctca cccggatggg tgttgctggg cgtaacctat tggatataca cgctgcaagt
     5881 taccgataac atgcgattca ttcgcaccct acccgggata cttgctgtca ttgctgcgcc
     5941 gcttagcttc cttcttttgg cgcttaaaac accttggcta attaactacg acatacagtc
     6001 ggtgatcgct aatccatggg ggttctattc agcctatgga ccattattta attatttctt
     6061 aatctggctg gaggtgtttt ttattgcctc gctgattatg atgttccggc gctaccgatc
     6121 aacggaggac ccgcgggtta aacgccaagc gctaactacc tcaatagccc tcgcaattcc
     6181 cctggcggta ggaagcttta ccgacggaat attcccggcc tttgacatta acgtaattcc
     6241 acttgcggtt accttgactt caatccaggc ggcaattacc gggtatgcca ttatcaaata
     6301 ccacatcttt accttcaata ccaaaatggt ggcatcaaat attttggctg gcatgaatga
     6361 agtcgtattg atccttaacc ggaaccatcg tattgaatac attaatgagc ggacatcaaa
     6421 tatcctagga tacccgatga gcaccctgct tggcgccaaa attgaaacct tgtttaccga
     6481 tgaccgccaa atgaaagatt ttaagaatat agccctgtcg ccgtcggcag acactaagtt
     6541 tcaaaatacc aatgtcgata tcaagaccgt tactgggggg cataaatcga tgttggcgtc
     6601 ttcggctcaa gttgttgatc ctgacacggg cgagcctgcc tacatcctgg taatgactga
     6661 cctcagtcag gttcagcgcg tcgtacttga aaacaagaat ctagccgaca ccaagctggc
     6721 catgattaat gccctcgaag atgcccgctt attggaggaa gaattgaaga acgaaaaagc
     6781 caatgtcgag aaaaaagtgg ttgagcgaac gcatcagcta gccgaggaac aagcgcgcct
     6841 aaatgcatcc attaacagcc tgcaggtggg atttatgatt ataaatggcc aaaaaaagtt
     6901 gcaatccatt aacgccgctt cctacgccat tttagaaacc aagccaagta aaagctttga
     6961 ttttgccgaa gtagccagca ggctaagcac tacgattgac ctcgagagtc aaattcagca
     7021 agtcgtaatc accaaaaaac cggtaaccct aaaagatttg atgtggcggg gtaaatatct
     7081 tcgcatcttc atttctccgg taattaccga aaaaaaggag gtgattggta cagttatttt
     7141 gcttcaagac attaccgagg agaagatatt gcagcgttcc cgcgaggaat ttttctcaat
     7201 cgcctcgcat gagttgcgaa ctcccttaac ggccattcgg ggaaacacct caatgcttca
     7261 aggttatttt ggggagatat tagaaaagaa tcccgatatg aaagaaatga ttaatgatat
     7321 ccatggttca agcgtccgat tgattactat cgtgaatgat tttctcgatg ccacccggct
     7381 tgagcaagga gggatgaaat ttactgatgt ggacgtcgac acgggcaagc tagccgccat
     7441 cgttattcag gatctgcaat cagtagccca agaaaaaaat atctatataa aaaatgaaat
     7501 aggtagccgg atg
//
LOCUS       DASVGB010000114         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_77629, whole genome shotgun sequence.
ACCESSION   DASVGB010000114 DASVGB010000000
VERSION     DASVGB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77629"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /gene="cgtA"
                     /locus_tag="VF272_03585"
     CDS             <1..554
                     /gene="cgtA"
                     /locus_tag="VF272_03585"
                     /inference="COORDINATES: protein motif:HMM:NF020830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEX7259988.1"
                     /translation="VHLIDVYSEDIVADYQTIQNELKQYKVDLSNKPQLVVLSKTEGM
                     PKEELQKKKRMLEKAAKVDVSEISAVAGRGIKPLLAKIVPLAEQARTVKDAEEAEQAI
                     PVIDEASQPNLWQVEHKEGVYYIGGEKLEGFARRTDWENDASVERLRDILRKNGIDRE
                     LRRLGAEPGAALDIGGHSFEWLG"
     gene            623..823
                     /locus_tag="VF272_03590"
     CDS             623..823
                     /locus_tag="VF272_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259989.1"
                     /translation="MSNMYPEWIQEQLDKIAAQSSNASDDRTAIRLAAVESRIIHHLG
                     RRMERVEAKLDFLISCCEVDEA"
     gene            843..918
                     /locus_tag="VF272_03595"
     tRNA            843..918
                     /locus_tag="VF272_03595"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:876..878,aa:Ala,seq:cgc)
     gene            1098..1280
                     /locus_tag="VF272_03600"
     CDS             1098..1280
                     /locus_tag="VF272_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259990.1"
                     /translation="MDPDTLDRVAANMPVPQQQLNDDAAKAATDARVAAIEQQKADAE
                     AAARAKDLANAARYGK"
     gene            complement(1349..1606)
                     /locus_tag="VF272_03605"
     CDS             complement(1349..1606)
                     /locus_tag="VF272_03605"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX7259991.1"
                     /translation="MAEQDNQNKGQGWYGDSEGHAKAGQKGGQKTAETHDSKFYSEIG
                     SKQGKENNPGNFANRPKEEVKEAASRGGQKSGSNRSDSTND"
     gene            complement(1792..>2114)
                     /locus_tag="VF272_03610"
     CDS             complement(1792..>2114)
                     /locus_tag="VF272_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7259992.1"
                     /translation="MKNALETTQVALTHRRMPAQTGSKGSYGHQHKTQEEVIFVISGN
                     VQVKVNDDIVNIGPKSAIRIAPGAIQGTWNEGPDDAEILIISNRVPNLGAEFASFPDF
                     WPAG"
BASE COUNT          541 a          480 c          533 g          560 t
ORIGIN      
        1 tggtgcatct tattgatgta tattcggaag acattgttgc ggactaccag accattcaga
       61 atgagcttaa acagtacaag gtggatctta gcaataagcc tcagctggtt gtattgagca
      121 aaaccgaggg tatgcctaag gaggaacttc agaaaaaaaa gcgcatgctt gaaaaagccg
      181 caaaagttga cgttagtgag atctcggcgg tggctggcag ggggattaaa ccgctactgg
      241 cgaaaatcgt accgctggcg gagcaggctc gaaccgtaaa ggatgccgag gaggccgagc
      301 aggcgatacc agtgatcgat gaggcttctc agcccaacct ttggcaggtg gagcataaag
      361 aaggtgtcta ttatattgga ggggagaaac tggaagggtt tgcccggcgc actgactggg
      421 aaaatgacgc ctcagttgag cgtttgcgcg acatcctgcg caaaaacgga atcgataggg
      481 agctccgccg cttaggcgct gaaccgggag ctgcgcttga tattggcggc cacagctttg
      541 aatggttggg ttaacgctgc ttgagttaat tcgaatttaa tagcactttg ctatcttcgt
      601 gaagtaacga cgaggagtta gcatgagcaa tatgtacccg gaatggattc aagagcaact
      661 agacaagatt gctgctcagt ccagcaatgc ctccgatgat cgtacggcga ttcgcttagc
      721 ggccgtagag tcgcggatta ttcaccattt gggccggcgc atggagcggg tagaggcgaa
      781 actggacttc ttgattagct gttgtgaagt agatgaagca tagtacaatt atcccggtgc
      841 ccggggatat agctcagctg atagagcgct gcattcgcat tgcagaggtc gggggtttga
      901 atccccctat ctccaccatt gtaattataa gtatgtagta tgtacaaatc cggcccagcg
      961 tctgttttgc aaaggacaac gcccttgttg tatctattaa tttttttaaa aagataaatt
     1021 taaccgacgc cttgtagccc tgaccaatga atttattacc caattgggta gactgaacta
     1081 catgaaagga ggtaattatg gaccctgata cattagatag ggtagccgca aatatgccgg
     1141 ttcctcaaca acagttaaat gatgacgctg ctaaagcagc aaccgatgcc cgagttgcag
     1201 caattgagca acaaaaggca gacgccgaag ctgccgcacg cgccaaagat cttgcaaatg
     1261 cagctcgtta tggtaaataa ctaattctga ccccgcaaat aaaaaagaga cgcttagcgc
     1321 gtctcttttt tattgaaaga ttaactaact agtcgttagt actatcgctt cgattgctgc
     1381 cactcttttg accgccgcgg ctggcggctt ccttaacctc ttctttaggg cggttggcaa
     1441 agttgcccgg attgttttcc ttgccctgtt ttgatcctat ctctgaatag aatttgctgt
     1501 catgggtttc tgcggttttc tggccgccct tttgcccagc tttagcatgt ccttccgaat
     1561 cgccgtacca tccctgtcct ttgttttgat tgtcttgctc tgccatatgc tatccccctc
     1621 ttaactttct ctttatgcga aacagaatat gggaattgat gcgggcgagc attataaggt
     1681 cttctagcat agccttcatc agaatggcta aatgctcctg tatacacata agatcgccag
     1741 caattgccta taataagggg tcattggcgc ttggcataat tacgaaaata actagcctgc
     1801 aggccaaaag tccggaaaag atgcaaattc ggctcccaaa tttggcaccc tgttggaaat
     1861 aattaaaatc tcggcatcat cgggaccttc attccatgtt ccctggatag ccccgggagc
     1921 aatgcggatc gccgacttag gacctatgtt gacaatatcg tcatttactt tgacctgcac
     1981 gtttccgctg atgacaaaaa taacttcttc ttgggtcttg tgctgatggc cgtagcttcc
     2041 cttgctgccg gtctgagctg gcatgcgccg atgagtgagg gctacctggg tagtttccag
     2101 agcattcttc attg
//
LOCUS       DASVGB010000115         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_512229, whole genome shotgun sequence.
ACCESSION   DASVGB010000115 DASVGB010000000
VERSION     DASVGB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512229"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF272_03615"
     CDS             <1..267
                     /locus_tag="VF272_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HEX7259993.1"
                     /translation="LTQKAVEKLITEIAPAFKNRQGGYTRVVKLGNRQGDNAKMAAVM
                     LTEAPKRAADTAPKADSASKKTSVAKAKKPIAKKPAAKKKVASA"
     gene            267..704
                     /gene="rplM"
                     /locus_tag="VF272_03620"
     CDS             267..704
                     /gene="rplM"
                     /locus_tag="VF272_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010199223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7259994.1"
                     /translation="MKTYSAKPSEITREWYVVDAKGMNLGRLATLIATHLMGKHKPMY
                     TTHIDCGDNVVVINAAEITVTGTKLTEKKYYRHSGYPGGIKETTLERLLQDHPERPIQ
                     QAVAGMLPKNKLQAERMKRLKVYPGSDHPHDPQSPKPLGAHHG"
     gene            697..1098
                     /gene="rpsI"
                     /locus_tag="VF272_03625"
     CDS             697..1098
                     /gene="rpsI"
                     /locus_tag="VF272_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7259995.1"
                     /translation="MADSKAHYYYGLGRRKEAIATARLYKGGGELMVGEQLAKDYFNN
                     DALIQLLEQPVSLAGQEKKLRITLNVSGGGKRGQADAARLAIARALDSMSDDLHTTLK
                     KAGYLTRDPREKERKKPGLKRARKAPQFSKR"
     gene            1100..1777
                     /locus_tag="VF272_03630"
     CDS             1100..1777
                     /locus_tag="VF272_03630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7259996.1"
                     /translation="MQVPNLTVLYEDDDVLAIDKPAGLVVHDAETATIQPTVADFARQ
                     QGVIDPDSDRPGIVHRLDKDTSGVMLIAKHPEAKAFLQRQFKQRQVEKSYIALVRGRL
                     RQEHAIIRLPIGRHRKQPVKRAVIPGGRASVTEYWVREVYAGASLVEISLHTGRTHQI
                     RVHFSYLGNPVIGDTFYGEKQLPRGLTRQFLHAAQISLELPSHKKVTIESPLPSELES
                     YLQNVSS"
     gene            1791..2666
                     /locus_tag="VF272_03635"
     CDS             1791..2666
                     /locus_tag="VF272_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7259997.1"
                     /translation="MKLLLHPAAETRVNAITARPGGSVLLYGPQNVGKRTIAYEIARR
                     LNCRGCEHDDCRSCRMVVGGNHPDVIRVSPDEKGKIGIEQVHQLVHDLQYERYDPAGQ
                     RVVVIDQAHRFTLPAQQALLKCLEEPPEATTMVVTCENLQALLPTVVSRCRPVHIPPV
                     SINDLTAFLKAEQPALSDTIDTVARLSEGAPGMAISLLNDPEALEQHNLYMLEAKKLF
                     ETEDLFEKLQLAAAMAIDGKLENHLSVLTSVSHGLIRQNKALGSIAAIERLRQRLRAN
                     VSPKTALEAMVVELA"
     gene            2663..3355
                     /locus_tag="VF272_03640"
     CDS             2663..3355
                     /locus_tag="VF272_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7259998.1"
                     /translation="MISLLVVALASFVLYRLLGGTPLRRKHRQTTGTTNLKIEELTEY
                     ASKLRASRNYPAAEKIYLQILKLDHRHAETYTRLGTLYLTMNNQTDAIECFQIATQLN
                     PTGGTFHNLGIAYYENRNSMKAIAALEKSVMFEPTVQRHLALAKALNKMGKGDQMINA
                     LEQAANLDPTTKVLWLLADAYAAARRTEDQERVYQRIRQLAPRDARMKALDKVTAVGA
                     ATRRTPVEKTKR"
     gene            3394..3468
                     /locus_tag="VF272_03645"
     tRNA            3394..3468
                     /locus_tag="VF272_03645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3426..3428,aa:Thr,seq:ggt)
     gene            3472..3557
                     /locus_tag="VF272_03650"
     tRNA            3472..3557
                     /locus_tag="VF272_03650"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3506..3508,aa:Tyr,seq:gta)
     gene            3582..3656
                     /locus_tag="VF272_03655"
     tRNA            3582..3656
                     /locus_tag="VF272_03655"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3614..3616,aa:Thr,seq:tgt)
     gene            3701..3862
                     /gene="rpmG"
                     /locus_tag="VF272_03660"
     CDS             3701..3862
                     /gene="rpmG"
                     /locus_tag="VF272_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013727979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7259999.1"
                     /translation="MPKRNRPYFALECTECHNRNYTTSKNPKNTTDRLELKKFCRHDG
                     KVTIHRESK"
     gene            3849..4055
                     /locus_tag="VF272_03665"
     CDS             3849..4055
                     /locus_tag="VF272_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX7260000.1"
                     /translation="MKANNAPKRLTKSRNRMLFGVCGGVAEYFNVDPTMVRLGFVALS
                     VLSAAFPGIIVYIIAAIIMPEPSE"
     gene            4091..4166
                     /locus_tag="VF272_03670"
     tRNA            4091..4166
                     /locus_tag="VF272_03670"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4124..4126,aa:Trp,seq:cca)
     gene            4345..>5075
                     /locus_tag="VF272_03675"
     CDS             4345..>5075
                     /locus_tag="VF272_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260001.1"
                     /translation="MAKSKIQKSQKLRLNENVKYSRKQAAIFILAFGVIGCIFVFNSI
                     AGPPSTSQPFVVKNEVFEDRNIPGGCVSEDDQLDIIGNNGYLAPGQTYTYTTKTPDCD
                     RNDRIIHMVVTWGPKSSRNMPTLELSSIVAANELWAGWGSTYFAAEDHVGKRIVATAK
                     PPTVNVKTSAGSASLCMFSKFNEPKNYTFTIKNTGTVTATNIAFSSTDRNDWPDFYLN
                     NCRTSDADRDGFSDAAEHYKAMWSAG"
BASE COUNT         1362 a         1245 c         1286 g         1182 t
ORIGIN      
        1 ttaacgcaga aagcggttga aaaattgatt accgaaattg ctccggcctt taaaaaccgc
       61 cagggtggct ataccagggt tgttaagctt ggcaaccgtc agggtgacaa cgctaaaatg
      121 gctgccgtaa tgctgactga agcgcccaag cgggcggccg ataccgctcc aaaagcggat
      181 tctgcgtcca aaaaaacttc ggttgccaag gctaagaaac caatcgctaa aaagccggct
      241 gctaagaaaa aggttgcgag cgcatgatga agacgtattc agcaaaacca tcggaaatta
      301 cccgcgaatg gtatgtcgtt gatgccaaag gcatgaacct gggccgtctt gccaccctaa
      361 ttgccacgca cttaatgggc aagcataaac caatgtatac cacgcatatc gactgcggtg
      421 ataacgttgt ggttattaat gccgccgaga tcaccgttac cggcaccaag ttaaccgaaa
      481 agaagtatta ccgccactcc ggttatccgg gcgggattaa agaaaccacc ttggaacgcc
      541 tgttgcaaga tcatcctgag cggccgatcc aacaagccgt tgccggtatg ttgccaaaga
      601 acaaacttca agctgagcgg atgaaacgac tgaaagtgta ccctggttct gatcatccgc
      661 acgatccgca atcacctaaa ccgctaggag cacaccatgg ctgattcaaa agcacattat
      721 tattacggct tagggcggcg caaagaggca atcgcaaccg cgcgtcttta caaaggtggc
      781 ggagaattaa tggttggcga acaactggcc aaggattatt tcaataatga tgcgcttatt
      841 cagcttttgg agcagccggt tagtttagct ggccaggaaa aaaagctgcg gataacgctc
      901 aatgtgagcg gcggcggcaa gcgtggtcag gccgacgcgg cccgcctggc aatcgctcgt
      961 gctctggata gtatgtcaga tgatttacac accacgctta aaaaagctgg gtatcttaca
     1021 cgagatccgc gtgaaaaaga gcgtaaaaaa ccaggtctta agcgagctcg taaagcgccg
     1081 cagttctcca agcgttagga tgcaggtccc taatcttacg gtcctgtatg aggatgatga
     1141 tgtgctggct attgataagc cggccggttt ggttgtgcat gacgcggaaa ccgctaccat
     1201 tcaaccaacg gtagctgatt ttgcccgaca gcagggcgtg attgatcccg atagcgaccg
     1261 cccaggcatc gtccaccgtc tcgataaaga cacctccggc gtgatgctca ttgccaagca
     1321 tcctgaggct aaagccttcc tgcagcgcca gttcaagcag cgtcaggttg aaaagagcta
     1381 cattgcgttg gtgcgcgggc gcttgcgcca agaacatgcc ataattcggc tgcctatcgg
     1441 ccgccaccgc aagcaaccgg ttaagcgtgc ggttattccg ggcggacgcg cgtctgttac
     1501 cgagtactgg gttcgcgagg tttatgctgg tgcctcgctg gtagagatca gcctgcatac
     1561 cgggcggacg catcaaatac gcgtacattt cagttatctg ggtaacccgg tgatagggga
     1621 taccttttac ggtgaaaagc agcttccgcg gggacttacc cgtcagtttt tgcatgcggc
     1681 gcagataagc ttggagctgc cctctcataa aaaagttacg attgaaagcc cgctgccatc
     1741 cgagctagaa tcgtatcttc aaaacgtaag tagttaggta tactaaaaac atgaaattac
     1801 tgcttcatcc tgccgctgag accagagtca atgccataac cgctcgtccc ggcggtagcg
     1861 tgctcctgta tggtccgcaa aatgttggaa agcgaacaat tgcctatgaa atcgcccggc
     1921 gtttaaactg ccgaggatgc gaacatgatg attgccggtc ttgccggatg gtagttggcg
     1981 ggaatcatcc ggatgttatt cgtgtttccc ccgatgaaaa gggaaaaatc gggatcgagc
     2041 aggttcatca gctggtgcat gacttgcagt atgagcgcta tgatccagcc ggccagcgag
     2101 tggttgtcat cgatcaagcg caccgcttta cgctaccggc tcaacaagcg ctgctgaagt
     2161 gccttgagga accgcctgaa gctacaacta tggtagtgac ctgtgagaac cttcaggctt
     2221 tgttacctac ggttgtatcg cgctgccgtc cggtccacat acccccggta agcattaatg
     2281 atttgacagc attcttaaaa gcggagcaac ctgccctttc cgatacaatt gataccgtgg
     2341 cgcggctgag tgagggggcg ccgggtatgg ccataagcct tttgaatgat ccagaagcgc
     2401 ttgaacagca caatctgtat atgctggaag caaaaaaact gtttgaaacc gaagatttgt
     2461 ttgaaaaatt gcaattggca gccgctatgg cgattgacgg caagctggaa aaccatcttt
     2521 cagtattaac ttcagtaagc catggtttga ttaggcaaaa caaggctctc ggcagtattg
     2581 ccgccattga acgcttgcgc caacgcttgc gggccaacgt gagtcctaaa accgcgctag
     2641 aagccatggt ggtagagcta gcatgatttc ccttttggtg gtcgctcttg cgagctttgt
     2701 cctctatcgg ctgctaggtg gtactccgtt gcggcgtaaa catcggcaaa ccaccgggac
     2761 cacaaacctt aagattgaag agctcacaga gtacgcgagt aagttaagag cttcgcgcaa
     2821 ctatcccgct gctgaaaaaa tttatttgca aattcttaag cttgatcatc ggcatgcgga
     2881 aacgtacacc cggctaggga ccctttattt gacgatgaac aatcaaaccg atgcgattga
     2941 gtgctttcag attgctaccc agcttaatcc caccggtgga acctttcaca acctgggaat
     3001 agcctattat gaaaaccgaa attcaatgaa ggcaattgca gcacttgaaa agtcggtgat
     3061 gtttgagccg acagttcagc gccatttagc ccttgctaaa gctttaaata aaatgggaaa
     3121 gggcgaccaa atgattaatg ctctcgagca ggcagccaat cttgatccaa ccacgaaagt
     3181 attgtggttg cttgccgatg cctatgccgc cgcccggaga accgaagacc aggagcgcgt
     3241 ctatcagcga attcgccaat tggcccccag ggacgcacgc atgaaagcgc tcgacaaggt
     3301 cacggcggtt ggcgctgcca cgcgccgtac accggttgaa aagaccaagc gctgatgtta
     3361 tgctaatcag gttaaacggc cagcaaacaa tatgcccagg tagctcagcg gtagagcact
     3421 tccatggtaa ggaaggggtc acgagttcaa tcctcgtcct gggctccata tgccgggtta
     3481 cccaagtggc caacgggggc tgactgtaaa tcagctggtg tacgccttcg ggagttcgaa
     3541 tctctcaccc ggcaccaata acggtccgac tatggccgtt cgccggcgta gctcaatggc
     3601 agagcaacag ttttgtaaac tgtaggttac gggttcaatt cctgtcgccg gctccatcaa
     3661 tcaaagtgat aaatgaaatt taatatctaa ggaaagcatt atgcccaaac gtaaccgccc
     3721 ctattttgcg ctcgaatgta ctgagtgtca taatcggaac tacaccacta gtaaaaaccc
     3781 taaaaatacc actgaccggc tggaactaaa gaagttctgc cgtcacgacg gaaaggtaac
     3841 aattcaccgt gaaagcaaat aacgcgccaa aacgtcttac caaaagccgt aaccggatgc
     3901 ttttcggcgt ctgcggcggg gttgccgagt attttaatgt cgacccaaca atggtccggc
     3961 ttggtttcgt tgcgctatcg gtactctcgg cagcctttcc cgggataatt gtttatatta
     4021 ttgctgctat catcatgccc gaacccagcg aataggcata atttagtcta taatgaggcc
     4081 ggtacatcat aggggtgtag ctcagttggt agagcaacgg tctccaaaac cgtgtgtcgt
     4141 gggttcaagt cctgccaccc ctgccaagct aaaacccaac ttttatggtt gggttttagc
     4201 ttggcagata tgtgagagcc actcaatcca gatgcgcagc ttcacccata aaaacgagtt
     4261 tgacaaaagg ctttgcaatg tgcttatgtt aaactagctg tctttatata gcgcgaaagc
     4321 aacctatcta aatcatgaaa tcaaatggcc aagagtaaaa ttcaaaaatc acagaagctg
     4381 cggcttaatg aaaatgttaa gtattcccgc aagcaagctg ctatttttat attggcattt
     4441 ggagtaattg gttgtatttt tgttttcaat tccattgcag ggccgccatc gacgagccag
     4501 ccttttgtgg taaagaatga ggtctttgag gatcgcaaca ttccgggcgg ttgtgtttca
     4561 gaggatgatc agctggatat aattgggaat aatggctatc ttgcccccgg ccaaacctat
     4621 acttacacta caaaaacacc tgactgcgat agaaatgacc ggataattca catggtagta
     4681 acgtgggggc caaaatcaag ccgtaacatg ccaacgcttg aacttagttc cattgttgcg
     4741 gctaatgaac tttgggctgg ctggggcagc acgtattttg ccgcggagga tcatgtcggc
     4801 aaaaggattg ttgcaacggc aaagcctcct acggtaaacg ttaagacaag cgccggatca
     4861 gcgtcgctgt gcatgttttc taagtttaat gagcccaaga attatacatt taccattaaa
     4921 aacacgggga ccgtaactgc taccaacatc gcgttttcat ctaccgacag aaatgactgg
     4981 ccagatttct atctcaacaa ctgcagaacc agcgatgccg accgcgatgg attttctgac
     5041 gcagctgagc actataaagc catgtggtct gctgg
//
LOCUS       DASVGB010000116         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_536259, whole genome shotgun sequence.
ACCESSION   DASVGB010000116 DASVGB010000000
VERSION     DASVGB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536259"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF272_03680"
     CDS             complement(<1..187)
                     /locus_tag="VF272_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HEX7260002.1"
                     /translation="MHRHGYQGKKLQLAAAPRRALIRGQVTSLVLHEQITTTEAKAKE
                     IAPYFERLVTKAKKGDLH"
     gene            complement(187..1131)
                     /locus_tag="VF272_03685"
     CDS             complement(187..1131)
                     /locus_tag="VF272_03685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7260003.1"
                     /translation="MLYDIQLPAIKALKEEGNRAVFTVEPLYPGYGMTLGNSLRRVIL
                     SSLGGAAVTAVKIDSVAHEFSTIKGVKEDVVEVILNLKKLRFQVYSDEPQYLILTKKG
                     SGMVTGADIKTNADVEIVNPEQHIATIDSPSTQVGMEIKVEKGRGYIPVEAREHEKLE
                     VGMIAVDAIYSPIKRVRYNVENTRVGQVTDLDRLVLEVETDGSISPREAVTQAAEILV
                     QHFAVVSGQSAPEAAQNTSVVDDVTESNASRIMIEEVNFSPRTTNALMNNDIRTMKEL
                     LRLSNAELRELKGFGAQAYEEVKSKIAELGLIRETEEA"
     gene            complement(1149..1766)
                     /gene="rpsD"
                     /locus_tag="VF272_03690"
     CDS             complement(1149..1766)
                     /gene="rpsD"
                     /locus_tag="VF272_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7260004.1"
                     /translation="MARDLTPIVKRSRREKTPLHPKAVKGLTLRNYGPGQHGQSSMRS
                     KLSQYAIQLREKQKVKRMYGLLEKQFRRVVAEAERRSGVTGDTLLELLEARLDNVIYR
                     LNWATSRQSARQLVTHGHVLLNGRRVDIPSIQVKPGDVIEVRPKSAANAYFETLKQEL
                     SKNQPTIGWLSLDAKKMTAKVSGTPVRAEIAEEINEQLIIEFYSR"
     gene            complement(1766..>2029)
                     /gene="rpsK"
                     /locus_tag="VF272_03695"
     CDS             complement(1766..>2029)
                     /gene="rpsK"
                     /locus_tag="VF272_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7260005.1"
                     /translation="AWASAGGAGFKGSRKSTPYAAGVAAERAAAAAKDMGLIKAHVLV
                     RGIGSGRESAVRALAASNIQVTSIKDTTPVAHNGCRPKKARRV"
BASE COUNT          455 a          548 c          498 g          528 t
ORIGIN      
        1 tgtgtaggtc gcctttttta gccttggtaa ccaagcgctc aaaataggga gcaatttctt
       61 tagccttcgc ctcggtcgtg gtaatttgct catgcagcac cagggaggtg acctggccgc
      121 gaatcagcgc ccggcgcggc gcggcggcca gctgtaactt ctttccttgg tatccgtggc
      181 gatgcattag gcttcctcgg tctcccgaat cagacccaac tcggcgatct tgcttttaac
      241 ttcttcgtag gcctgagctc caaagccttt cagttcgcgc agctcggcat tgctaagacg
      301 tagcaattct ttcatggtgc gaatatcatt gttcatcaac gcgttagtag tccgcggcga
      361 aaaattaact tcttcaatca taatccggga agcattcgac tcagtaacat cgtcaacaac
      421 cgaagtgttc tgcgctgcct cgggagcaga ttggccggaa acaacggcaa aatgttgaac
      481 caatatttcg gccgcttgag ttaccgcttc gcgtggagag atagagccat cagtttcaac
      541 ttccaaaact aagcggtcca agtcggtaac ttgaccaacg cgggtgtttt ccacgttgta
      601 gcgaacacgc ttaattggag agtagattgc atcaacagca atcatcccga cttccaactt
      661 ttcatgctcg cgagcctcca ccgggatgta gccgcggcct ttttcaacct ttatttccat
      721 gccaacctgg gtgcttggcg agtcaatggt agcgatatgc tgctcgggat tgacgatttc
      781 gacatcagca ttggtcttaa tatcagcgcc ggtgaccatt ccggaaccct ttttagtaag
      841 aatcagatac tgaggttcat cactatagac ttgaaagcgt agctttttaa ggttaagaat
      901 gacctctact acatcttctt tcacgccttt gattgtcgaa aattcatgag caacggaatc
      961 aatcttaacc gccgtgacag ctgcgccacc caagctggaa aggattaccc ggcgcaacga
     1021 gttcccgagg gtcatgccgt atccggggta aagcggctca accgtaaaaa cagcgcggtt
     1081 gccttcttcc ttgagagcct taattgctgg cagttgaatg tcgtataaca ccttaaaatc
     1141 cttccttgtt agcgtgaata gaactcaata atgagttgtt cattaatttc ttcggcaatc
     1201 tctgccctaa ccggtgtgcc ggaaacttta gcagtcattt ttttggcatc aagactgagc
     1261 cagccgatag tcggctgatt cttgcttaat tcctgcttca aagtttcaaa ataggcgttt
     1321 gcagcgctct ttggccgcac ctctataaca tctcccggct taacttgaat cgatggaata
     1381 tccacgcgcc gtccgttcag gagaacgtgg ccatgcgtca ccagctggcg ggctgactgg
     1441 cgggatgttg cccagttgag gcggtaaata acattatcca gacgggcttc cagcagttca
     1501 agcagcgtat cgccggtaac gccggagcgg cgctcagctt ctgcaaccac tcggcgaaac
     1561 tgcttttcca gcaacccata catgcgtttt actttttgct tttcacgcaa ctggattgca
     1621 tactggctca gtttggagcg catacttgat tgcccatgct gcccgggacc ataattgcgc
     1681 aaggttaagc ctttaaccgc cttggggtgc agcggcgttt tttcacggcg cgagcgctta
     1741 acaatagggg ttaaatcgcg tgccattaga cgcgccttgc cttctttggt cggcaaccgt
     1801 tgtgagctac cggagtggta tctttaatgc tggtcacctg aatatttgag gcagccaaag
     1861 cccggaccgc cgattcgcgt ccgctgccaa taccgcgcac taagacgtgg gctttaatca
     1921 gtcccatatc cttagcagca gctgctgccc gctcggctgc cacgccggcc gcatagggag
     1981 ttgatttgcg cgaaccctta aatccggcgc cgcccgcact ggcccaggc
//
LOCUS       DASVGB010000117         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_529996, whole genome shotgun sequence.
ACCESSION   DASVGB010000117 DASVGB010000000
VERSION     DASVGB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529996"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VF272_03700"
     CDS             <1..1061
                     /locus_tag="VF272_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7260006.1"
                     /translation="KHITIGGAIVGIGIETNSFRKGFVHDNVLEAEVLLPDGEVVTCN
                     AHQHAALFHGLPNSYGTLGYILRATIKLHAVKPYAILTTKRYTTVPTFIAAMGKAAKN
                     TAIDYLESLAYAKDELYLTTIQHSDHPQNLISIYGSTIFYKRISRPGTLSLTTRDYLF
                     RYDPEWFWAMPDSGIFKLFRRLAPPSLRNSSFYARYANFQGRLAARLPFIPGENPSME
                     LLIQDWEVPWKHAADLLAYAFDNLDLAGRPLMSAPVKTPGLATSYPMQKNQFYLNLGS
                     YSYVKKQPGKPTYHNTKMMDAFTFAHAGLKMLYSTTMLPEADFNRIYNGPSYKKVKAK
                     YDPQQLLPTLYEKTVKAY"
     gene            complement(1123..1566)
                     /locus_tag="VF272_03705"
     CDS             complement(1123..1566)
                     /locus_tag="VF272_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260007.1"
                     /translation="MSARPAVTCGINWAVNGDRLIIFGVEALQGIPCKLREMANRSGI
                     CSVLDELNAHIEKIIAGRQWAEIRCIAIRPELAGLMWAEGSLVLSDLVDSTEFELNNK
                     VGEVIRFTLEKLNAPHVLLVFPAPSPGCENGLVPFCLDSDDCFVG"
     gene            complement(2280..2365)
                     /locus_tag="VF272_03710"
     tRNA            complement(2280..2365)
                     /locus_tag="VF272_03710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2330..2332),aa:Leu,seq:tag)
BASE COUNT          575 a          649 c          597 g          545 t
ORIGIN      
        1 ttaagcacat cactataggg ggtgccatcg tcggcattgg tattgaaacc aacagttttc
       61 gcaagggctt cgtccatgac aatgtactgg aggccgaggt attgttaccc gacggcgaag
      121 tcgtaacctg caacgcgcat cagcatgctg ctttatttca tggtctgcct aattcgtacg
      181 gaactttagg ctatatctta cgcgccacca taaagctgca tgcggttaag ccctatgcaa
      241 ttttgaccac caagcgctac accacagttc caacctttat tgcagctatg ggaaaagccg
      301 ctaaaaacac tgcaattgat tatcttgaaa gcctagcata tgcaaaagat gaactttacc
      361 tcaccactat ccagcacagc gaccaccccc aaaacctaat cagcatttat ggatcgacca
      421 tcttctacaa aagaatcagc cggccaggca cattatcgct caccacgaga gactatctgt
      481 ttcgctatga tccggagtgg ttttgggcga tgccggactc gggcatattt aagctattca
      541 gacggctcgc cccgccttca ctacgcaatt caagttttta tgcccgctac gctaatttcc
      601 agggccggct agccgccagg ttgcccttta ttccgggaga aaatcccagc atggagctgc
      661 ttattcaaga ctgggaagtg ccttggaagc atgcagccga tttgcttgct tatgcgtttg
      721 ataatcttga ccttgccgga cgtccgctca tgtcggcgcc agtaaaaaca cccggcctgg
      781 cgacttctta ccctatgcag aaaaatcaat tctaccttaa tctaggcagc tacagttatg
      841 ttaaaaaaca gcccggcaag ccaacctacc acaatactaa aatgatggat gcctttacct
      901 ttgctcatgc gggcttaaaa atgctgtatt cgacaaccat gcttcctgag gccgacttca
      961 accgcatata taatggccct agctataaaa aggtaaaggc caaatacgac ccgcagcagt
     1021 tgctgccgac gttatacgaa aaaaccgtta aagcctacta ggggataagt gcagtggacg
     1081 gggaaggcag tgcctccccc ggtgtcccac ttggtgcggt gttcagccga cgaagcaatc
     1141 gtcggagtcg agacagaaag gcacgaggcc gttctcgcac cccggagaag gtgcggggaa
     1201 gacgaggagc acgtgcggtg cgttcagctt ttcgagcgtg aagcggatca cctcgccgac
     1261 cttgttgttc agctcgaact cggtgctgtc caccagatcc gagagcacca gtgacccttc
     1321 ggcccacatg aggccggcaa gttccgggcg tatggcgatg caacgaatct ccgcccattg
     1381 ccgcccggcg atgatcttct cgatgtgagc gttaagctcg tcgagcactg agcaaatgcc
     1441 cgaacggttg gccatctcgc ggagcttgca gggaatgccc tggagggcct ccaccccgaa
     1501 aatgatgaga cggtcgccat ttaccgccca gttgataccg caggttactg ctggtctggc
     1561 tgacacgatc ctacctccgt ccgagggata cgggctttgt gggacaaatc caaggagaat
     1621 caccttgaac cgcactataa taccatattt cttgtgctaa ttcaatgagt ttattacggg
     1681 ccgctcactc tgcgaatctc aaccggcatc gttcccgagc ccagaccttc aagctggcta
     1741 tagcttcctc gagataggtc tattacccgg ttggtgcctg gtgttggtcc ataatcatta
     1801 accaaacacg taacttccct gttgttgcga aggtccctaa cccgcaaata tgtgcctcgg
     1861 ggaaagcgcg tcgaggcgca ggtaagagcg tcgggctggc gcaatcccag agcataccag
     1921 gtggcaacac caatctggtc cggcgctttc gacccttcgc gtttttgaaa cgcagcggcc
     1981 tgggaggcac ggatataggc atccaagtga gggtcgtttc caggaggtgg cagcagctcc
     2041 gctgcccgca aaatatgggt cgagctaaac gctactgcag caataaacaa gagcagtgca
     2101 accacaagat tacgtttagc gtgcgtgcgc gtccggcgct ggtgatgagg cgccttagca
     2161 gccgccgacc gctttaaaaa cttgatcttg gcggtcttcg tgcgtggctt cggggtagga
     2221 tatttgatat gcataagtag tactaatacg cttatgctta ttatagcatt taaaatatct
     2281 ggtgggcgag agaggacttg aacctccacg gggtttcccc caccagctcc taaggctggc
     2341 gtgtctacca tttcaccact cgcccg
//
LOCUS       DASVGB010000118         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_79963, whole genome shotgun sequence.
ACCESSION   DASVGB010000118 DASVGB010000000
VERSION     DASVGB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79963"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF272_03715"
     CDS             complement(<1..225)
                     /locus_tag="VF272_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260008.1"
                     /translation="MTGARCRTSGISVEPGDLDSPASRFDDMLVCQQAACSIGGNPSA
                     HRTYWSEHRWAGWNQWEQSTPKRTSVPRAPA"
     gene            complement(355..1044)
                     /locus_tag="VF272_03720"
     CDS             complement(355..1044)
                     /locus_tag="VF272_03720"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEX7260009.1"
                     /translation="MNRSLIFLAMGAITAMWMLRVGITGSFRFDFIVWNIVLAFVPLL
                     VEPLFGIIVRKTKGALAKMGKLVVSAVWLLFLPNAFYILTDLMHLNSRVSVNARDDSR
                     TYTLLYDRGDGLYVFDSLLLVAATVFGAYVGGLALLHAWKYFRKRMPQLSAGAILALI
                     MTLSAVGVYIGRFGRWNSWDGLIHPHYVISDLFTSLITPATQGRFLLILLTMLIFQLS
                     SVLIVQRIHKR"
     gene            complement(1083..>2087)
                     /locus_tag="VF272_03725"
     CDS             complement(1083..>2087)
                     /locus_tag="VF272_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260010.1"
                     /translation="PSPQAASDTTPPARLQNLSAKEEDGLVSLSWDSLGDASNYKIER
                     SLDEKDWVFLAEIDAPNYDDTSTEFATKYFYRVAAVDAAGNIGEFATTSVIVKAFTAN
                     VLPGETAVITSEDSRISYEIEFETFGEDATCTVAKSSVKAPKNTVIGPYVLSCKNKAG
                     DDLNEYKKPIVVVLQPAEKNSAGKLNVVSLSNGKSSTLPSVINKDQNNLSITISQPMV
                     LAAVKKGSGALLWLLLVAALAGIAGGYLVLRRRANGPAFNDGPLASPYIPTAKPKPPS
                     SETPHYVADPLEEHPLQSARDRLNRIPSADKMPPPAQPTPPPNPTNNPDVTKTNDPDK
                     "
BASE COUNT          520 a          481 c          546 g          540 t
ORIGIN      
        1 agcgggtgct cgtgggacgc ttgtacgctt cggcgtcgac tgctcccact ggttccatcc
       61 cgcccaacga tgctcactcc agtaggtgcg gtgagcggag ggattaccgc cgatactgca
      121 tgcggcctgc tgacagacaa gcatgtcgtc gaatcgagaa gcaggcgaat ccagatcgcc
      181 gggctcaacg gatatgccgc tggtacggca tctagcgcct gtcatcacca tgcgtcgcgc
      241 atagagagtt cccttcaaat ggagcgatct atgtgcggcg ccggttcatt tggaattatt
      301 taatttatag aatataccga aaaattatag gtagtgattg aatgaggcta ctagctatct
      361 tttgtgtatt cgctgcacaa tcaacacact gcttagctga aaaatgagca tggtaagtag
      421 aattaataag aagcggcctt gggttgctgg tgtgatgagg cttgtaaaca ggtcagatat
      481 gacgtagtgg gggtgaataa ggccgtccca gctgttccac cgtccaaaac gaccgatgta
      541 tacgcccact gcagacaacg tcattattag ggctagaatc gcgccagccg atagttgtgg
      601 cattcgttta cgaaaatatt tccaggcatg cagaagtgcc aatccgccta cgtaggcgcc
      661 aaacacggta gctgcaacga gcagcagcga atcaaacaca tataagccat cgccgcgatc
      721 atatagcagc gtgtacgttc gagagtcatc acgggcatta accgaaacgc gcgaatttaa
      781 atgcatgagg tcggttagaa tatagaatgc gtttggaaga aatagcagcc agacggcgga
      841 tacaaccagt tttcccattt ttgccagggc tcctttcgtt ttgcggacta tgatgccaaa
      901 gagcggctcc accagcaatg gaacgaaggc taagacgata ttccagacga taaagtcaaa
      961 acggaagctt ccagtaatgc caacgcgcag catccacata gcggtaatag cgcccatagc
     1021 tagaaagatt agtgatcgat tcatatagtt ataagactac catctgctag cagcggataa
     1081 tgctatttgt cagggtcatt ggttttggtg acgtcgggat tattggtcgg gttaggcgga
     1141 ggagtcggtt gagctggagg cggcatctta tcagcagatg gtatccggtt taagcggtcg
     1201 cgtgcgctct gcaatggatg ttcttcaagt gggtctgcta cataatgagg agtctctgaa
     1261 gagggcggct tcggtttagc tgtaggtatg tatgggctag caagcggtcc gtcgttgaaa
     1321 gccgggccat tcgcacgtcg gcgtaacaca aggtatccgc cggcgattcc agcaagagcg
     1381 gccacaagca atagccacag caatgctccg cttccctttt taaccgcggc gagaaccatt
     1441 ggctgactta tggtaatgct caggttgttt tggtctttgt tgataaccga tgggagtgtg
     1501 gacgacttgc cattgctgag actaacaacg ttcaatttac cagctgaatt tttttcagca
     1561 ggctggagta ctaccacaat cggttttttg tactcattaa gatcgtcgcc tgctttattt
     1621 ttgcatgata gtacgtaagg cccgattacc gtatttttag gtgcttttac actgctcttg
     1681 gctaccgtgc acgttgcatc ttcaccaaag gtttcaaatt caatttcgta gctgatacgt
     1741 gaatcttcag aggtaattac cgctgtttca ccgggaagaa cattggctgt aaaggctttt
     1801 actatcacgc tagttgttgc gaactcgcct atattgcctg ccgcatcgac agccgctaca
     1861 cggtaaaaat acttcgttgc gaactcagtt gaggtgtcgt cataattcgg tgcatcaatc
     1921 tccgcgagaa aaacccagtc tttttcatca agtgagcgtt caatcttata gtttgatgcg
     1981 tctcccaagc tgtcccacga cagtgaaact aaaccatctt cttccttcgc tgaaaggttt
     2041 tgaagtcttg cgggtggcgt agtgtcggat gctgcctgag gcgaggg
//
LOCUS       DASVGB010000119         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_795123, whole genome shotgun sequence.
ACCESSION   DASVGB010000119 DASVGB010000000
VERSION     DASVGB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795123"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..608
                     /locus_tag="VF272_03730"
     CDS             138..608
                     /locus_tag="VF272_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HEX7260011.1"
                     /translation="MNIYVTAGAGSASTSLGAFDQALLAAGIANYNLIYLSSIVPHGS
                     TIVTGERPQLSGEWGDRLYVVIAQARTSIPNVEAWAGLGWAQHEETGKGLFVEHHGAS
                     KSTVEREIEQSLETLMQGRPNEKFGPIQMQVEGLRCETAPVCVLVAATFITENW"
     gene            complement(623..2302)
                     /locus_tag="VF272_03735"
     CDS             complement(623..2302)
                     /locus_tag="VF272_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7260012.1"
                     /translation="MNDYTEDMSLADDIKQFLKGEVDDSAESRAFYSHDASLFEVTPE
                     VIVYPQDSHDLGKLVRYVADHKADHPLLSITARSAGTDMSGGAVNDSIIADFSRHFNT
                     IASVSRTTAHVQPGVFYRDFEVETLKHDALLPPYPASRSLCTVGGMVNNNSGGEKSLE
                     YGKTENFVTALTMILADGNEYIFGPLTRQQLHKKLKLENFEGDLYRKTFALVDDHYEE
                     IKAAKPHVTKNSTGYNLWDVWDRETGVFDLTKLVVGAQGTLGFVTDITFRLVPSRPHS
                     GLLVVFLKDLNRLGELIPLVLKHKPASFESFDDHTLFLAIKFMPSFAKTLGYFGLLKL
                     MISLIPDGLILLRGIPKLVLMIEFNGNSVDEVRQKIQSMHRELEHKHVYYEIEGEEEN
                     PTEASSQKFWIMRRESFNLLRSKVKDKHTAPFIDDLVVPPEHLTDFLPKLRVIIKKYN
                     LLATIAGHMGDGNFHIIPLMRMEDPQERAKLRPAMKEVNELVLRYGGVLSGEHNDGMI
                     RGPWLEQAYGPEVTKLFREVKDIFDPQHIFNPHKKANADWGWSMSHIRQSF"
     gene            complement(2332..>2674)
                     /locus_tag="VF272_03740"
     CDS             complement(2332..>2674)
                     /locus_tag="VF272_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260013.1"
                     /translation="IKGGVYEIELLGTSKRMNGFEPFKVSYVQRVEAASQGAGLLSGI
                     SQGWLEFIVSLGLVGLLAVLVWGRHSSSPLAANDRERFHNPLLHYGFYVVLAGLAILI
                     LFDATAFVHAH"
BASE COUNT          723 a          710 c          589 g          652 t
ORIGIN      
        1 atatgcagga tttagggctt gaaggcttac gccaatcaaa aaccatgcta cacccgattg
       61 attttttaaa aaaatatacc ataagcctca cgagtccata acaactattt aagaaagtat
      121 ggttataata ggcaggtatg aatatttacg ttaccgcagg cgctggcagc gcctcaacct
      181 cgctcggggc cttcgaccaa gccctgctag cagccggaat cgccaattac aatttaattt
      241 acttaagctc gattgtcccc cacggaagca cgattgttac cggcgagcgg cctcagctat
      301 cgggtgaatg gggcgaccgc ctatatgtcg ttatcgctca agcacgaacc tcaattccta
      361 atgtggaagc ctgggccggc ctggggtggg cgcaacatga ggaaaccggc aagggcttat
      421 ttgtcgagca tcatggcgcc agcaaatcca ccgttgaacg cgagattgag cagagtctgg
      481 aaacgctgat gcaaggccgt ccgaacgaaa agtttggtcc gattcaaatg caggtcgaag
      541 gattacgctg cgaaaccgca ccggtatgcg tattggtagc cgccacattt atcactgaga
      601 actggtagac ctggtttaac cgctaaaacg attggcggat atggctcatc gaccagcccc
      661 aatcggcatt ggctttttta tgcgggttaa atatatgctg aggatcgaaa atgtccttaa
      721 cctcgcgaaa cagcttagta acctctggac catacgcctg ctccagccac ggcccgcgaa
      781 tcatgccgtc attatgttca cccgacaaca ccccgccata acgtaagacc agctcattca
      841 cctctttcat cgccggccgc agcttagccc gctcttgtgg atcctccatc ctcataagcg
      901 gaataatatg aaagttgcca tcccccatgt gcccggcaat agtcgctaac aaattgtatt
      961 ttttgataat gactctcagt tttggcaaaa agtcggttaa gtgctctggt ggtacaacca
     1021 aatcgtcaat gaacggcgcc gtatgcttat ccttcacctt gctgcgcaag agattaaagg
     1081 attcccggcg catgatccaa aatttctgac tgctggcttc cgtcggattc tcttcttcac
     1141 cctcaatctc ataatagaca tgtttgtgtt caagttcgcg atgcattgat tgaatttttt
     1201 gccggacctc atccacgcta ttaccattaa actcaatcat caggacaagc ttggggatac
     1261 cgcgaagcaa gattaagccg tcgggaatca agctaatcat cagttttaat aagccgaaat
     1321 agccgagagt ttttgcaaat gacggcatga atttgatagc aaggaagagg gtgtggtcat
     1381 caaaagactc aaagctggct ggcttgtgct tcagcaccag cgggataagc tcaccaagcc
     1441 ggttaaggtc ttttaaaaat acgacaagca ggccggagtg cgggcggctg gggaccagac
     1501 gaaacgttat atcagttaca aatcccaggg ttccctgtgc ccctacaacc aactttgtta
     1561 gatcaaatac cccggtctcg cggtcccaca catcccataa attatagccg gtagaatttt
     1621 tggttacatg cggcttggcg gctttaattt cctcataatg atcatcaacc aaagcaaagg
     1681 tttttcggta gagatcgcct tcaaaatttt ccaatttcaa cttcttatgc agctgctggc
     1741 gggttaacgg gccgaaaata tactcattgc catcagctag aatcattgta agagctgtca
     1801 caaaattttc ggttttacca tattccaacg acttttctcc gccggagtta ttgttcacca
     1861 tgcccccaac ggtacacaaa gaacgcgaag ccggatacgg aggtaataag gcgtcgtgtt
     1921 tcagtgtttc aacctcaaag tcacgataaa acacccctgg ctgcacatga gcggtcgtcc
     1981 ggctcaccga cgctatagtg ttaaaatgcc ggctaaaatc agcaatgatc gaatcattta
     2041 cggctccgcc cgacatatcg gttccggcac tgcgcgcagt tattgaaagc aatggatgat
     2101 cggctttgtg atcggctaca tagcgcacca atttcccgag gtcatgacta tcctgcggat
     2161 agactatcac ctccggcgtt acctcaaata aactggcgtc atggctgtag aaagcacgcg
     2221 attccgcgct atcatctacc tcgcctttta gaaactgctt aatatcgtcg gctaggctca
     2281 tgtcttcagt atagtcattc ataaacctta acgccactat caaggctatg cttaatgggc
     2341 atgcacgaat gccgtagcat caaacaagat caaaatcgcc aaccccgcca atactacata
     2401 aaagccgtaa tgaagcagcg gattatgaaa ccgctcgcgg tcgttagctg caagaggcga
     2461 actagaatgt cggccccata ccaacaccgc caacaatccc accagcccta agctcacaat
     2521 aaactccagc catccttggg aaatgccgct caataagcct gccccttgcg atgctgcctc
     2581 aacccgctga acgtagctta ccttgaaggg ttcaaagcca ttcattcgct tgctcgtacc
     2641 aagcagctct atctcataca ccccgccctt gata
//
LOCUS       DASVGB010000120         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_332384, whole genome shotgun sequence.
ACCESSION   DASVGB010000120 DASVGB010000000
VERSION     DASVGB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332384"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VF272_03745"
     CDS             complement(<1..958)
                     /locus_tag="VF272_03745"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX7260014.1"
                     /translation="MLVASVPVGSTDTRANTGATTLDGWYRVAFIVLSGLGFIALALQ
                     LGLSAGMAGRIMPGVSVAGMAIGGMDRVEARQLLAKPLQQHTVNLAVGTTTYKVSSRD
                     IGAVYDLDATLDQAFLVGRDTAFVPLQIMHAIKSQPVAYSYQLNRQAEAAFIEKVITG
                     SGQAPVDAAIIVNDGVPTVQPDVDGRSLSKKAVRQAIAAQLDSFSSRAIRLEPSIQHA
                     RIRVANIQMAIEQTKQLLAVPVTITYLDKTFIPTPAQKSQWISFQKSAEEETPALIPG
                     ISKDGVKNYLQSLAVQINVNPVNRKINVQNGDSQETQAGKDGL"
     gene            complement(1045..>2292)
                     /locus_tag="VF272_03750"
     CDS             complement(1045..>2292)
                     /locus_tag="VF272_03750"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260015.1"
                     /translation="PVEAAPGALERAVSLLRDSGDTEPVMAVADYVLQPSVATKIVAR
                     IERINQVLGLQLTADAISGHLKTLAFQVDNSDDPKVLAVTSPWWRTDIQSEDDLAEEV
                     IKLVGYDHVPATLPLWQPTQINFDKTWPQRWKAKATLKSAGLFEVATYSFISAHQIDF
                     LGRLPQDFLALANPLSTEQAYLRTDLLASLLSVAERNRMYSKNFGMFEFSKVYVPKSK
                     GELPEEPIHLGVLVRSEHHSYKQVKAVLDRLSRVFNVPIQVVPHKLEESAAYPNRAGY
                     LQLGDTHIGWIGQLHPDLANQLKLDGEVAYMELVWEPFLAAAKPKQSAVRGRFPTAMR
                     DVAVVVDRAVTWDELAAALEGHTITFINDYYGDELGSEKKSIAFQLTVQYPDRTPTDT
                     DAEKAVNEVVGKLKQHLKAVLRA"
BASE COUNT          538 a          603 c          554 g          597 t
ORIGIN      
        1 gcaagccatc ttttccggct tgggtttctt gggagtctcc attctggacg tttatcttgc
       61 gattcaccgg attaacgttt atttgaacgg ccaacgactg caagtagttc ttaaccccgt
      121 ccttactaat acccggaatt agtgcgggag tctcttcttc ggcacttttt tgaaaactaa
      181 tccactgact tttctgggca ggcgtcggaa taaatgtctt gtccagataa gtaatggtta
      241 ccggtaccgc aagtagttgc ttggtttgct cgatagccat ctgaatgttg gctactctga
      301 tccgcgcatg ttgaatgctt ggctctagcc gtatcgcccg gcttgaaaag ctatctagct
      361 gagcggcaat tgcctgccga acggcttttt tacttaaaga gcgtccatca acatcgggct
      421 gcaccgttgg tacgccatcg ttaacgataa tcgcagcatc caccggcgct tgaccgctac
      481 cggttattac cttttcaatg aaagccgctt cggcttggcg atttaattgg taggagtagg
      541 caaccggttg gctttttata gcatgcataa tttgcagtgg cacgaatgct gtgtcgcgtc
      601 ccactaaaaa tgcctgatcg agtgttgcat ccaggtcata tacagcgcca atatcgcgag
      661 agcttacctt atacgtcgtg gttccgactg caagattgac ggtatgctgc tgaagcggct
      721 tggctagcag ctgcctggcc tctaccctgt ccattccgcc aattgccatt ccagctacgc
      781 tgactcccgg cataatccgg ccggccatac ctgcgcttaa gccgagttgt aaggccagcg
      841 caatgaatcc aagtccgctc agtacaatga atgctacccg gtaccaaccg tccagagtgg
      901 ttgctccggt attggcacgc gtatcggtag aaccaacagg aactgaggct accagcatga
      961 tgtagctacc ttatgcagaa gagaatcttc ccgcttatgg aataattata gctcttatgc
     1021 ttagtttttg gctactgttt ttacttaagc ccgtaataca gcctttagat gctgcttgag
     1081 cttcccaact acctcgttaa ccgccttttc ggcatcagta tcggttggcg tacgatcggg
     1141 gtattgaaca gttagctgga aagctatgga tttcttttca cttccaagct catcgccata
     1201 ataatcatta atgaatgtga tagtgtgtcc ctctaaagct gccgccagct catcccaggt
     1261 tacagcgcga tctacaacca cagctacatc ccgcatagca gtaggaaaac ggccgcggac
     1321 tgccgactgc ttaggcttgg cggctgctaa aaacggctcc catacaagtt ccatgtaggc
     1381 tacttcgcca tccagcttaa gctgattggc caaatctgga tgcaactggc cgatccagcc
     1441 aatgtgggta tcgccaagct gaagatatcc ggcccggttt ggatacgccg cggattcttc
     1501 aagtttgtgc ggaactacct gaatgggaac attaaatact ctagaaagcc ggtcaagcac
     1561 ggctttcact tgcttatagc tatggtgctc agaccgcacc aacaccccaa gatgtatcgg
     1621 ttcttcaggt agctcaccct tcgacttcgg aacgtagact ttggaaaatt caaacatgcc
     1681 aaaatttttc gagtacatcc gattgcgctc agcaactgat aacagacttg ccagtagatc
     1741 ggtgcgcagg tatgcctgct cggttgaaag cgggttggca agggctagaa agtcttgagg
     1801 caagcgccca aggaaatcaa tttgatgggc gctaatgaac gaataggtcg caacctcaaa
     1861 caagccggca gatttaagtg tcgcttttgc cttccatcgc tgaggccagg ttttgtcaaa
     1921 attaatctgc gtcggctgcc acaatggaag agttgctgga acatggtcat agccaaccag
     1981 cttaatgact tcttctgcca gatcgtcttc agactgaata tcggttcgcc accaaggact
     2041 ggttaccgcc aaaaccttgg ggtcatcgct gttgtctacc tggaatgcaa gggtctttag
     2101 gtgcccgctg attgcatcgg cagtcagctg aagtccgagc acctggttaa tacgctcgat
     2161 gcgagcaaca atcttggttg ccaccgacgg ttgcagcaca taatcggcca ctgccatgac
     2221 cggttccgta tcacctgaat cccggagcaa gctcactgcc cgttcaaggg ctcccggtgc
     2281 cgcctccacc gg
//
LOCUS       DASVGB010000121         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_795725, whole genome shotgun sequence.
ACCESSION   DASVGB010000121 DASVGB010000000
VERSION     DASVGB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795725"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /gene="dnaE"
                     /locus_tag="VF272_03755"
     CDS             complement(<1..2225)
                     /gene="dnaE"
                     /locus_tag="VF272_03755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7260016.1"
                     /translation="MALFISSPEEVAERWKHVPEALKNTVAIADACNVELQLDQILIP
                     TFPTPNNETEHDYLQKLCWQGAMWRYGDIAHEDMHLQTEEKARKVLAPEIIERLDYEL
                     GIISKMGYEGYFLIVADFINWGKNQGIIFGPGRGSAAGSIVAFSMNITDLNPLEYDLL
                     FERFLNPDRISMPDIDIDIQDTRRGEVIDYVTEKYGQDRVAQIITFGTMAARNAVRDT
                     GRALGMSYAEVDAIAKKIPAPVQGRHIPLATSIKQDPELRGEYDSNPRAKNLIDLAMK
                     LEGTIRSNGVHAAGVVIAADDIVKFTPLLRAQKGGICTQYSMGPIEQLGLLKMDFLGL
                     SNLTIIKNTLRIVKKVYSKDIDIAEIPLDDKKTYELFSRGDTTGLFQFESAGMKRYLR
                     SLKPTRFDDLIAMNALYRPGPMQWIEDFIDRKHGRKKVEYLHPTMQAALEPTHGIIVY
                     QEQVMQISKEMCGFTGGQADSLRKAVAKKQPVEMAKMKKAFIEGAMKTSGADKTLMED
                     FWSQLEAFAAYAFPKSHSACYALIAVQTAYLKANYPSAFMAALMTSNYDNIDKISMEI
                     SECRRMGINVLSPDINESFLEFGIVPDSNDIRFGMAAIKNVGTGAINTILQAREAGGP
                     FKSVEDFAKRVSASECNRKVWEALIKSGAMDGFGERSQLLHNLDSITSYASKAQKNAL
                     SGQIDIFGSLGEDSMPGLRLELPERVIPAREQLGWERELLGLFLSQHPLDDYEGYLAD
                     HT"
BASE COUNT          564 a          594 c          499 g          607 t
ORIGIN      
        1 gtatgatcgg ccaaatagcc ttcataatca tctagcggat gttgggagag aaacaaacca
       61 agcagttcac gctcccatcc caactgttcg cgcgccggaa taacccgctc gggcaattcc
      121 aatcgcaagc cgggcatact atcctcgcca agcgacccga aaatgtcgat ttggccggaa
      181 agtgcattct tctgggcctt cgaagcatat gatgtaatac tgtctaaatt gtgcaacaat
      241 tgtgagcgtt cgccaaagcc gtccatggct cccgatttaa tgagcgcttc ccacacttta
      301 cgattacatt cagaggcgga aacccgtttg gcaaaatctt ccacgctttt gaacggcccg
      361 ccagcttcac gagcctgcag aatagtgttg atggcaccgg tgccgacatt tttaatggct
      421 gccataccaa aacgtatatc attagaatcg ggcactatgc cgaactctaa aaagctttcg
      481 ttaatatccg gcgacaacac attaatgccc atccggcggc actcggaaat ttccatcgaa
      541 attttatcaa tattgtcata gtttgaagtc atcaaggcag ccataaaagc gctgggatag
      601 ttagctttta aatacgctgt ttgtaccgca atcagggcat aacaggccga gtgtgatttc
      661 gggaaggcat aagccgcaaa cgcctccagt tggctccaga agtcctccat gagcgtcttg
      721 tctgccccgg aagttttcat tgcgccttca ataaaggctt ttttcatttt ggccatttcc
      781 accggttgct ttttggcaac cgctttacgc aaactatccg cttggccgcc ggtaaacccg
      841 cacatttctt tagaaatttg cattacctgt tcctggtaga caataattcc gtgggttggt
      901 tcaagcgctg cctgcatcgt aggatgaaga tattctacct tctttcgccc atgtttacgg
      961 tcgataaagt cttcaatcca ctgcatcgga cccgggcggt aaagcgcgtt catagcaata
     1021 agatcatcaa accgggtagg tttcagtgac cgtaggtagc gtttcattcc tgctgactca
     1081 aactggaaaa gtccggttgt atcgcccctt gagaacagct catacgtttt tttgtcatca
     1141 agcgggattt cagcaatatc aatgtccttg ctgtagactt ttttcacaat ccgtaaggta
     1201 tttttaataa tagtaaggtt agatagacct aggaagtcca tttttagcag gccaagctgt
     1261 tcaatcggcc ccatcgagta ttgggtgcaa ataccacctt tttgagcgcg taataaaggg
     1321 gtaaatttca cgatatcatc ggctgctatc actacgccgg ctgcatgcac gccattggag
     1381 cgaatggttc cttccaactt cattgccaga tcaatcagat tcttagctcg aggattagaa
     1441 tcgtactcgc cgcgcaactc cggatcttgc ttaatcgagg ttgccaacgg gatatggcgg
     1501 ccttgaaccg gtgccggaat ctttttggca atggcgtcaa cctcggcata actcataccc
     1561 agtgcccggc cggtatcgcg cactgcatta cgcgccgcca tggtgccgaa cgtaataatc
     1621 tgtgctaccc gatcttgccc atacttctcg gtaacatagt cgatgacttc gcctcgccga
     1681 gtatcctgaa tgtcaatatc aatatcaggc atgctaatgc ggtcgggatt aagaaagcgt
     1741 tcaaagagca ggtcgtattc cagcgggtta aggtcggtaa tattcataga gaacgctaca
     1801 attgacccgg ctgcactccc gcgtcctggc ccaaaaataa taccctggtt tttaccccag
     1861 ttaataaagt cggcaacaat caggaagtac ccttcatatc ccattttgct gataattccc
     1921 aactcgtaat ctagccgctc aataatctcc ggcgccaaaa ccttgcgagc tttttcctcg
     1981 gtttgcaaat gcatatcctc gtgggctatg tcgccgtagc gccacatggc accctgccaa
     2041 cataacttct gcaaataatc atgctcagtt tcattatttg gcgtagggaa agtcgggatc
     2101 aatatttggt cgagctgcaa ctccacatta caagcgtctg caattgccac cgtgttttta
     2161 agcgcttccg gcacatgctt ccagcgctcg gctacttctt ccgggctcga tatgaagaga
     2221 gccatatcca tttccatccg gtcggtatca ccaatcgtct tgcc
//
LOCUS       DASVGB010000122        12257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_828728, whole genome shotgun sequence.
ACCESSION   DASVGB010000122 DASVGB010000000
VERSION     DASVGB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12257
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828728"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5928)
                     /locus_tag="VF272_03760"
     CDS             complement(<1..5928)
                     /locus_tag="VF272_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260017.1"
                     /translation="MMPEGRTSSQSSDATMPSAGSTPSVEPTTPGIAVPPPEHHARFF
                     GRSATFIAAVITLLVFFGFGVLLFAAQLKGEHDPASATGLSYKVTSLSLSEFARTGEL
                     SLDKTQQLQINGQLRINNSAVITPGNEPSAPLPGQLYMSQADNQLYYFDGTAYVNLAD
                     NSQKVTSLGGLSGDIALGSGLSVIGGVLSSTVAPSSVVSVQGLTGAVSFSAGGGIALN
                     GTAISNIGVTGLTGTVGQINVSQATGNVILTLPQSIATSATPSFSGINLASALTVANG
                     GTGGTAFTANGVLLGNGAGGLTATTSGSAGQCFISNGAAAPSFQACPGGGGGNISSGT
                     SQTAGTLTKFDSVLNTITDSLVSEVGTVVTIGGDLSVSGIVSGNGSGLTNVNAAQLNG
                     QLGSFYQDASNLNAGIVSDTRLSTNVTLQGNSFNGANQLVQLNASTQLPAVSGTNLTN
                     LNASSVSSGTLPVIYGGTGANSFAANGVLYGNNTGALLATAAGTTGDCLMAGVGGVPS
                     FQTCPVGGNISSGTSQTPGALTKFDSTTNEITDSLLTETAGLVTASGNFTITGTPTLS
                     ALGAGILHSSAAGVVTSSAINLNSADVTNTLQIGNGGTGAITAGAARTNLGAAASGAN
                     ADITSLSGLTTALSVTQGGTGQNTYTDGQLLIGNSVGNTLTKATLTAGSGVTIVNGNG
                     TITISAPSTGSCSTCANVTLSNLSSPTAINQSLLTDGSAAIDLGSATNAFRDLYLANQ
                     LSILESGASPTFRGNLVVSDLAANQTYTLNEGGTVVTSGNVASFATTSVTAGSGLTGG
                     GTTGALTVNVGAGTGIQVNANDLEVLYGAVAGTAVQGNTSITVSAGTNLNGGGAITLG
                     AGGSVTLNTVNNPTFSTSVTTPLLNLSNSGFNGALQPTTLTGNQTYTLPDSSGTVAVS
                     ASGNIALSALGNISFAGTLPVGSGGTGQNSYTDGQLLIGNSTGSTLSKSTLTAGSGIT
                     IVNGNGSITISAPSAGSCPTCANTALSNLSSVAINTSLLPGSSTIDLGSTANPFRTGY
                     FNTDVKILEDGATPTFHGILNVADLSADQTYTLNEGGTVVTSGNVTSHATTGVTAGSG
                     LTGGGTTGVLTVNIGAGNGITVNPNDITLALQANKGLEVDVNGLSLIDCADTQILKYT
                     TATNQWGCAPDAGGSGVGDDISVNGVNATAANFLDVAATGTVAGTTWSLNTVSNPDDI
                     TVAISNASDTQAGVVTTGVQTFAGAKTFNGAITAGAGIDLGAQTLSGTTAVINFTNFD
                     VSSAGAVTAVGLDSGTGLIQGSGGLTVSAGTTSITGTTNINTSGAAATSVGTGTGTLA
                     LGNAAGATTLTGSSSSTFVLNGTTVSAAEFNILDGGIDLSTETSGNYVATITAGGGLT
                     GDVASHGSTPTLAVGAGTGIQVNANDIAVLYGAIAGTAVQGNTSITVSAGTNLIGGGS
                     ITLGSGGSVTVNTVNNPTFSTSVTTPALILSGGGSSGTLQVASLGQATAYTLPDPGAS
                     TATICLSSGNCAGAGGGVTGTGTAGTLAKFTASGVIGDSLVSETGSAVTVSGTLAITG
                     ASTVTLGTSTSATGSLIFNNAANGNTLTLQSGATGSNLTFTLPTADGGSGQCLKTNAS
                     GVLSFGDCLTGAGGGGGGVASLNGLSGTLSLANASGVGSTVTIDNASTSVKGIAQFNA
                     TNFSASAGTINTIQDIATTASPTFDTLTANTELRVLEDGGSPTNYGTLNVATLSADQT
                     YTLNEGGTVITTGNVANNAVTSVSAGDGLTGGGGPGSVTLNVVAGNGITVNPNDVTLA
                     LQTNKGLEVDANGLSLIDCGDNQILKYSTGTNQWSCAADAGGSGLGDNITVNSTTATD
                     ANFLDVAATGTVTGTAWSINTVSNPDDITIAISNASATVAGAVTTGIQTFAGAKTFNS
                     QITAGAGIDLGAQTIQGTTAVINFTNFDVSSAGAVTAVGVDAGTGLIQNTGGLTTTGT
                     ASINTTGTAASSIGNATG"
     gene            complement(6104..6934)
                     /locus_tag="VF272_03765"
     CDS             complement(6104..6934)
                     /locus_tag="VF272_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260018.1"
                     /translation="MKVAATSWMPGGLRLFAGVFLLGLAMTPWLTLAAAISHGYQGDS
                     NLVVGTLVAQSNDDKSRVVAADNKRPDSLIGVVVENRTSTLEITDPSDDIQVATSGKT
                     TVIVSDIAGRIKAGDLIAASPIQGVGMKAVVAGKSIGVSQSDAPANGSKTTTVTSTDG
                     KTQTVQISTIPVVLEVAYFVPPAEKSPFPVFIQKFANILAGKPVAIIRVLIATVLVVT
                     AMVVIGVILFSGIRSTLISVGRNPLARGSIFQVLWRILATVLVILIGAFGAAYLVISG
                     "
     gene            complement(7011..7086)
                     /locus_tag="VF272_03770"
     tRNA            complement(7011..7086)
                     /locus_tag="VF272_03770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7051..7053),aa:Pro,seq:cgg)
     gene            complement(7164..7616)
                     /locus_tag="VF272_03775"
     CDS             complement(7164..7616)
                     /locus_tag="VF272_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260019.1"
                     /translation="MRDELWLQQILDETWDRYFSEVPQQNIVRIQFGRRAKRQLGSIR
                     LDRRDPTISIITMNGLFRSPEIPEFAVIATLVHELIHYAHGFNSPLDRKHAHPHAGGV
                     IRAEYAERGLEDLYVKQKKWLKQNWPTIVAKNFPSPSVRHRAPRPFWL"
     gene            complement(7609..10497)
                     /locus_tag="VF272_03780"
     CDS             complement(7609..10497)
                     /locus_tag="VF272_03780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7260020.1"
                     /translation="MDLSELNPEQLQAVTYGAGPILVVAGAGTGKTRVITHRIAHLIE
                     TKAARPNQVLALTFTEKAAREMADRLYDLIGWESYQVPVVTFNAFGAELLGAYASHIG
                     RSTHGGLINDTQKALLLQQHLDRVSLSYYGPHASMFEFLEGVVEYLGRLQNAGVSAKA
                     YAAYAASLKPDKAGLHPQDITEQHDLSQLYSLYESLKAETGTYDFNDQLAIPLQILQQ
                     KSNLAERLTKQYHYVLVDEYQDTNRLQDELLRTFIPQDGNIFAVGDDDQAIYGFRGAQ
                     ISNILDFTDHYKPKKPLVLTQNYRSGQQILDAAYRLIVHNNPDRLEAKLGLNKHLQAQ
                     HKQASVAFQAYPKAVDELEGLREALSAQIAGGATPASIAVLSATHVPLLRLAKQLRAH
                     ELPYALSTRVSIFDQPELNQLWYLLQWVGLKADPESIAHVMLSRFIGWDSQAYRELLR
                     KARAELVEVEDVLRTDDSEHSRKAIAKLDEWRSWMPKIPVSVLTYRLMFETEVKDQLL
                     AEAKQSSYRIRRVFEDLGQLLTQMQDYETVAADPTLLGYLAHFPKPPTIEVTEPQGSA
                     EGIQLLTIHASKGLEFDSVYLLQCTRANWSPRQNRGWLVPESLRPQTELPPDHELRRL
                     LYVAATRARKQLVVSAPMMTASGAKQTVSPLVEELLGEIASVTSQDKLSSDVISGTIS
                     ALQRFYPLRQKQETRLPFETTDGWLELNVSALGGYDFCPYDFYLEHVLSIAQPFGPQL
                     HFGTLLHKLLQDYYQAKLHGEEITQEELLGRLQEQWTDQGYQKRQQAETAKQLAATTL
                     VNFLQREQKIAASAEINIMASELPFNLEIKESKLRINGRIDAVFTTPEGIELRDFKTG
                     HKTDPEALSRAAKTSFQLRTYALAYQEMTDRLPDRVSLDYVVTGTIGSSELSPRIIDN
                     HRAKLTDLAAAIRSRDFTPKPSNVHQCAAIRYYGTGEEDERQLEAARA"
     gene            complement(10511..12121)
                     /gene="argS"
                     /locus_tag="VF272_03785"
     CDS             complement(10511..12121)
                     /gene="argS"
                     /locus_tag="VF272_03785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7260021.1"
                     /translation="MMIDDVQNAVRSAINSKYSIDDISLVITYPEPKFGDFSTNAAFL
                     LAKRLNQPPGEVATELAAAIAADTGNTIAEAEAVNGFINMRMDNHFWVGQLLSITSEF
                     GSSQLGKGRRVEVEFISANPTGPTTIGNARGGYIGDTLANVLAHSGYAVTREYYFNNA
                     GTQIAKLLESVKVAAGLIRAEAVQYKGEYIHELASDFKAELAAKSDDELKELITQAIL
                     ERWIRPAIDKMGIHFDSWFNERDLVEGGQLEETIGQLRKQNLVYDHEGALWLDTAKLG
                     LSREARVLIKSTGDPTYLAPDIAFHDDLFGRRSFDSAIKILGADHVDQFPSVKAAVAI
                     LHPNRQLEVVVAQWFRLLKAGKEVKISKRLGQFVTIEELIDEVGTDVARFMTLMRSAD
                     TGMDFDLSLAKEQSQKNPLFYVMYSYARANSILDQASERGLTPISTVERLSEYEVALV
                     RHMSKFPRLVADIAHSYEVHHLTFFGMQAAKLFHDLYESERIIDLDKTEACRRLYVIK
                     QYIVFMEAYSKVLGITPVKRMERDTETA"
     gene            12164..>12257
                     /locus_tag="VF272_03790"
     CDS             12164..>12257
                     /locus_tag="VF272_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEX7260022.1"
                     /translation="MKLLAKNRRATYDYQIEDKLVAGLVLSGAEV"
BASE COUNT         2970 a         3121 c         3199 g         2967 t
ORIGIN      
        1 accggtagcg ttgccaattg agctggcagc ggtacccgta gtgttgatgc ttgctgtacc
       61 agtggtagtc aggccgcccg tattttggat aagtccggtg ccagcatcaa caccaaccgc
      121 ggttacggct ccggcgctcg aaacatcaaa attggtaaag ttaatgacgg cggttgtgcc
      181 ttgaatggtt tgggcgccaa ggtcaatacc agctccagcc gtaatttggc tgttgaaggt
      241 cttggcgccg gcaaaggtct gaataccagt agtaaccgcg ccagcaacag tagctgaagc
      301 atttgagatg gcgatcgtaa tgtcgtccgg gttggagacc gtattaatgc tccaggcagt
      361 accggttacc gtaccggtag ccgctacatc gaggaagttg gcatcggttg cggtggtgct
      421 attaaccgtt atattgtcac caaggccgct gccgccagca tcggcggcac aactccactg
      481 attcgtcccg gtggagtatt taagaatttg gttgtcgcca cagtcgataa ggcttaaacc
      541 gttggcgtca acttccaagc ccttgtttgt ctgaagggct aaagtaacat cattagggtt
      601 aacggtgatc ccattaccag ctaccacgtt taaggtaacc gagcctgggc cgccaccacc
      661 ggtaagtcca tccccagcag aaacagacgt aacagcgtta ttggctacat taccggttgt
      721 gattacggtc ccgccttcat tcagggtata agtctgatct gccgacaggg ttgcaacatt
      781 taacgtccca taattagtcg ggctgccgcc atcttccaag acccgaagct cagtattggc
      841 cgttaaggtg tcaaaggttg gactcgcagt ggtggcaatg tcctgaatag tattaatggt
      901 gccggcacta gcactgaagt ttgtggcatt aaactgagcg atacccttca cactagtaga
      961 ggcattatct atagtgacgg ttgagccaac accggaagca ttggccaaag acaaagtacc
     1021 cgagaggcca ttgagtgagg ctacgcctcc cccgccaccg ccggcaccgg tcagacagtc
     1081 gccaaagctc aatacgcccg aagcattcgt cttcaaacac tggccgcttc cgccatcagc
     1141 cgttggaagc gtaaatgtca aattcgaacc ggtggcaccg gactgcaatg tgagcgtatt
     1201 accattcgct gcattattaa atattaatga accagtggct gaggtgctgg tgccaagcgt
     1261 aacggtgctg gcgccggtaa tagccaaggt tccactgacg gttacagccg agcctgtctc
     1321 acttaccagg gaatcgccaa tgacgcccga agcagtaaat ttagctagcg tgccggcagt
     1381 tccggtaccg gtaactcccc cgccggctcc ggcacagttt ccgcttgaaa gacagatagt
     1441 ggccgtagag gcaccggggt caggcaacgt atacgcagtt gcctgaccga ggctggcaac
     1501 ctgaagcgta ccgctcgaac caccacccga caaaatgagg gccggtgtgg tcaccgatgt
     1561 tgagaaggtt ggattgttaa cggtattaac ggttaccgat ccgccagaac ctaatgtaat
     1621 ggagccgcca ccgataagat tggtcccggc tgaaaccgtg attgacgtat ttccctgtac
     1681 ggcagtgccg gcaattgcgc cgtagagtac ggcaatatcg tttgcattaa cctgaattcc
     1741 ggtcccagcg ccaaccgcta gggtcggggt ggagccgtgg gaggctacat cgccggtgag
     1801 tcccccgccg gcggtgatgg ttgcgacgta gttgccggag gtttcagtgg agagatcgat
     1861 gccaccgtct aagatgttga actcggcggc ggagacggta gtgccgttta agacaaaggt
     1921 tgaggaggag gagccggtta aggtagtggc accggcagcg ttgccgaggg ccaaggtgcc
     1981 ggtaccggtg ccaaccgagg tagctgccgc gccgctggta ttaatgttgg tggtaccggt
     2041 aatagaggtg gtgccggctg aaaccgtcag gccgccgctg ccctggatga ggccggtgcc
     2101 ggaatccaag ccgacggcgg ttacggctcc ggcgctggag acatcgaagt tggtgaagtt
     2161 aatcacggcg gtggtgccgg agagggtctg ggctccaagg tcaatgccag ctccagcggt
     2221 aatggcgccg ttgaaggtct tggcgccggc gaaggtctgg acgccggtgg tgacgactcc
     2281 ggcttgggtg tctgaagcgt tggagatagc gacggtgatg tcgtcggggt tggaaacggt
     2341 attaaggctc caagtggtac cggcgaccgt accggtagcg gccacatcaa ggaagttggc
     2401 ggcggtagcg ttgacgccgt taacgctaat gtcgtctccg acgcctgagc cgccggcatc
     2461 aggggcgcag ccccattggt tggtagctgt ggtgtactta aggatttggg tgtcggcgca
     2521 gtctatgagg gagaggccgt tcacatcgac ttctaagccc ttgttggctt ggagagctaa
     2581 ggtaatgtcg ttagggttaa cggtaatgcc gttaccggcg ccaatgttga cggtcaagac
     2641 gccggtggta cctcctccgg tgaggccaga tccggcggtg actccggtgg tggcgtggct
     2701 tgtcacgttg ccgctggtta ctacggtgcc gccttcatta agagtatagg tttggtctgc
     2761 cgaaagatcg gcgacattaa ggatgccatg gaaggtggga gttgcgccgt cttcgagaat
     2821 ttttacgtca gtattaaagt agccggtacg gaaaggattg gctgttgaac caaggtcgat
     2881 agtcgagcta ccaggcaaaa gcgatgtatt tatggcaaca ctactcaaat ttgatagcgc
     2941 tgtatttgca catgtggggc aagatcccgc agaaggagcc gaaatggtaa ttgagccatt
     3001 gccgttcacg attgtaatac cgctgccagc cgtaagcgta gacttggaca aggtgctgcc
     3061 agtgctgttg cctattagga gttgtccgtc ggtgtaactg ttttgaccag ttccaccgct
     3121 tcctacggga agagtaccgg cgaaactaat gtttcctaaa gcgcttagcg caatgttacc
     3181 tgaagccgaa accgctactg ttccagaaga gtccggcaat gtgtaggttt ggttaccagt
     3241 gagcgttgtt ggctgcaagg caccattgaa gcctgagttc gaaagattca agagcggtgt
     3301 tgtcacgctg gtgctaaagg tagggttatt aaccgtattt aaggtaacgg atccgccagc
     3361 tccgagagta attgctcctc ccccatttag gttagttcct gctgaaaccg taatagacgt
     3421 gttgccttga accgcagttc cggcgaccgc tccatagagt acctcaagat cgtttgcatt
     3481 tacttgaata ccggttccag cgccaacgtt cacggtaagt gctccggttg tgccgccgcc
     3541 ggttaggccg gacccagcag taaccgaagt cgttgcgaaa ctggcaacgt taccactagt
     3601 tacgactgtg ccgccctcat taagcgtgta ggtttgattg gccgctagat ctgatacaac
     3661 aaggttgccg cgaaaggttg gcgaggctcc actttcaaga atgcttagct gattggctaa
     3721 ataaagatcg cggaaggcat tggtagcact accaagatca attgctgcac taccgtcggt
     3781 cagcaagctt tgatttatag cggttggtga tgaaaggttg gaaagcgtaa cattagcgca
     3841 agttgaacat gaaccggtcg acggagcaga aatggtgatc gtgccatttc cattcacaat
     3901 cgttacacca ctgccagcgg ttaaggttgc cttggtgagg gtattaccaa cactgttgcc
     3961 aatcagtagc tgaccgtcgg tgtaggtgtt ctgaccggtg cctccctggg ttactgaaag
     4021 cgcagtggtt aggccagaaa ggctggttat atcagcgtta gcaccgctgg ctgcagcacc
     4081 caagttggta cgggctgcac ctgccgttat ggcgccggta ccgccattac caatttgcaa
     4141 tgtatttgtt acatcagctg aattcaggtt aatggctgaa gatgttacaa cgcctgcggc
     4201 gcttgagtgt aaaatacctg ctcctaatgc tgaaagcgta ggcgtaccag taatcgtaaa
     4261 gttgccgctg gcagttacca agccagccgt ttcggttaag agtgagtcgg tgatttcatt
     4321 ggtagtgcta tcaaatttgg tgagagcccc tggggtctgc gaggtaccag aagaaatatt
     4381 gccgcctact gggcacgttt gaaagcttgg tacgccgcca actccggcca ttaagcagtc
     4441 gccagtcgtt ccggcggcag tcgcgaggag agcgcccgtg ttattgccat aaagcacacc
     4501 gttggcggcg aagctgttcg caccggtacc tccgtagata accggcagtg tcccggaaga
     4561 aacgctggaa gcatttaaat tggtaaggtt cgtaccgctg accgccggta gctgcgtaga
     4621 agcgttcaat tgtaccagct gattagcccc attgaagcta tttccctgga gagtaacatt
     4681 tgtgctcaat cgcgtatccg atactatgcc agcattaaga ttactggcat cctgataaaa
     4741 actaccgagc tgaccgttta actgcgcggc gttaacattg gtgagaccgg aaccattacc
     4801 cgaaacgatg ccgctaacac ttaagtcgcc gccaatcgtt acaacggttc cgacttccga
     4861 aaccagtgag tcggtaatag tattcaaaac gctatcaaac ttggttaacg ttccagccgt
     4921 ttgggaagtt ccggaagaaa tattcccgcc tcctcctccc ggacaagctt gaaagcttgg
     4981 agcggcggca ccgttggaaa taaaacattg tcctgcacta ccactggttg tagccgttaa
     5041 tccaccggca ccgttgccaa gtaggacgcc gttagccgta aatgccgtac cgccggttcc
     5101 gccattagct accgtaagcg cacttgccaa gttaattccg gaaaaactgg gggttgccga
     5161 ggtagcaatg ctctgcggta gtgtaagaat tacattaccg gttgcttgac tgacatttat
     5221 ttggccgaca gtaccggtta acccggtaac accgatgttc gaaatagccg taccgttaag
     5281 cgcaataccg ccaccagcgc taaagctaac ggctccggtc aacccttgaa cgctaaccac
     5341 cgagctgggt gctacggtgc tgctgaggac tccaccaatc acactcaaac cgctgccaag
     5401 ggcaatatct ccgctcaaac ctccaagcga cgttactttc tggctattgt ctgccaagtt
     5461 tacataagcg gtgccatcga agtagtacag ctgattgtcg gcctgcgaca tgtagagctg
     5521 ccctggcagc ggtgcagaag gctcgttgcc aggtgtaata accgctgaat tattaattct
     5581 tagctgaccg ttaatttgga gctgctgggt cttatcgaga cttaactctc cagtacgggc
     5641 aaactcggaa agcgacaggc tggtaacctt gtagctcaac ccggtagcag aagctggatc
     5701 atgctctccc ttgagttgag ctgcaaacag aagtacccca aacccaaaaa atactagcaa
     5761 ggttattaca gcggcaatga acgtggctga acgaccaaaa aatcgggcat gatgctccgg
     5821 aggcggaacc gcaataccgg gagtagttgg ctccacgctt ggagtcgaac cggccgacgg
     5881 cattgtagca tcagaagact ggctcgaagt cctcccttcc ggcatcatag tccaaaacct
     5941 ctgaacattt cagatgtaac tccgcttcca cacattggtt ttaattatag cggttatgct
     6001 tagagaatgc aaacctgtta cgcttatgcg cgcgcgtact attatactgc gccgtatttc
     6061 agagcttctt aaccacgtgc ggaaataaaa atctaacctg tttttagccg ctaatcacca
     6121 aatatgcagc gccgaaggca ccaatcaaaa taaccaacac ggtagccaaa atccgccata
     6181 aaacttgaaa aatactgccc cttgcgagcg gattacgccc taccgagatc aacgtactgc
     6241 ggattccgga aaaaagtatt acgccaatca cgaccatagc tgttacaacc aatactgttg
     6301 caatcaatac ccggataatc gcaaccggct ttcccgccag aatattcgca aatttttgaa
     6361 tgaacaccgg gaaggggctc ttttctgccg ggggtacaaa ataggccacc tcgagaacca
     6421 ccggtatggt ggagatttga actgtttggg ttttgccatc ggttgaggta acggtggtgg
     6481 ttttagagcc attggcaggc gcgtcagatt gggacacgcc gatactcttg ccggctacca
     6541 cagccttcat gccaaccccc tggataggtg atgccgctat cagatcacca gcttttatac
     6601 ggccggcaat atccgataca atgacagtcg tcttgccgct ggtagccacc tgaatgtcat
     6661 cgcttggatc ggtaatctcc agggttgagg tgcgattttc aacgaccact ccgatcaagg
     6721 aatcaggccg cttattgtcg gcggcgacca cccggctctt atcatcgttc gactgagcca
     6781 ccaaagtacc aactaccaga ttactgtctc cttggtagcc gtggctaata gctgccgcca
     6841 aggtaagcca aggagtcatt gccaatccaa gcaaaaaaac gccggcaaat agccttagcc
     6901 caccaggcat ccaagatgta gcagccactt tcaccccgag cctccactca tgaagtgtat
     6961 tataccagtt acgcttgaag tttggccagt ttggcgcacg gaattagtgc tggtcggggt
     7021 gagaggactt gaacctccgg cctcagcaac ccgaatgctg cgcgctacca actgcgccac
     7081 accccgtaac ctgaagctat tctagcgtaa aaggctccac gggctcaaga atgtattgtt
     7141 tttgatgagt tttgtagtaa taattacaac caaaatggac gtggtgctcg atgtctaaca
     7201 gatggtgacg gaaagttctt ggcgacgatt gttggccaat tttgtttaag ccattttttt
     7261 tgcttcacgt agagatcttc gagtccccgc tcagcatatt cggctcgaat taccccgccg
     7321 gcatgagggt gggcatgttt tcgatccaaa ggtgaattga aaccatgggc ataatgaatc
     7381 agctcgtgaa caagtgtggc aattacggcg aattccggta tttccgggga gcggaataga
     7441 ccgttcatgg tgatgatcga aatggttggg tcgcggcgat caaggcggat tgatcctagt
     7501 tggcgcttgg cccgcctacc aaactgaata cgcacaatgt tttgctgggg cacctcgcta
     7561 aagtagcggt cccacgtttc atcaagaatt tgttgcaacc acagctcgtc acgcacgggc
     7621 agcctccagc tggcgctcat cttcctctcc cgtaccgtag tatcgaatag cggcgcactg
     7681 atgcacgtta gaaggcttgg gagtaaaatc tcgtgaacga atagccgctg ccaaatctgt
     7741 cagctttgcc cgatgattgt cgatgattcg gggagaaagc tcggagctac caattgttcc
     7801 ggtaacgaca taatcaagag aaaccctatc cggcagccta tccgtcattt cctggtatgc
     7861 cagtgcgtag gttcgaagct gaaagctagt ttttgcagca cggctaagcg cttcggggtc
     7921 ggttttatgc ccagttttaa agtcgcgcag ttcaataccc tccggtgttg tgaaaactgc
     7981 atctatgcgg ccatttatgc gtagttttga ctccttaatt tccagattaa acggcagttc
     8041 acttgccata atgtttattt cggcactagc ggcgattttt tgctcacgtt gtaaaaaatt
     8101 cacgagtgtt gttgcggcca gctgcttggc agtctcggct tgctgccgtt tttgataccc
     8161 ttgatcggtc cactgctcct gtaaacggcc cagcaactcc tcctgcgtaa tctcttctcc
     8221 atgcagttta gcttgatagt aatcttggag aagcttatgg agaagagtgc caaagtgtaa
     8281 ctgagggcca aagggctgag caatggatag tacatgctcc agataaaagt catacggaca
     8341 aaaatcgtaa ccgcctaatg cactgacatt taattccagc cagccgtcgg ttgtttcaaa
     8401 cggcagcctg gtctcctgct tctgacgtaa tggataaaaa cgttgtaatg cactaattgt
     8461 cccgctgatt acatcggatg atagcttatc ttggctggta actgacgcta tttccccaag
     8521 caactcttca accagaggtg aaactgtttg cttggccccg ctggccgtca tcattggcgc
     8581 gctaaccacc agctgcttgc gtgctcgggt tgccgctacg tagagcaagc gccgcagttc
     8641 atggtcgggc ggcaattcgg tctgcgggcg taagctttca ggcaccagcc accctcgatt
     8701 ttggcgaggc gaccaattcg cccgagtgca ttgcagtaga tacacgctat caaactccaa
     8761 acctttcgat gcatgaatgg taagaagctg aatgccttct gcgctgcctt gcggctcggt
     8821 tacctcaatc gttggcggtt tgggaaagtg cgccaagtag ccgagtaggg ttgggtcggc
     8881 ggccacagtc tcgtaatcct gcatttgagt aagcagttgc ccaagatctt cgaatacccg
     8941 ccggatccgg tagctactct gttttgcctc tgccaagagc tgatccttta cctcggtctc
     9001 aaacattaat cggtaggtaa gcacgcttac cggaattttt ggcatccagc tgcgccattc
     9061 atcaagcttc gcgatagctt tgcggctgtg ttctgaatca tcagtgcgta agacatcttc
     9121 tacttccacc agctcagcac gagctttacg cagcagttct cggtatgcct gactatccca
     9181 accgataaac cggctgagca taacatgggc aatgctttcg gggtcggctt ttaagccaac
     9241 ccactgcagc agataccaaa gctgattaag ctccggttgg tcaaagatgc taaccctggt
     9301 agagagcgca tacggcagct catgggcgcg caattgtttg gctaagcgca gcagcggtac
     9361 atgggtggcc gaaagaacgg caatgcttgc aggagtagca cctccggcaa tttgggcact
     9421 caaggcttca cgcaaaccct caagttcatc gacggctttt ggataagcct gaaatgcgac
     9481 gctggcttgc ttatgctggg cctgcaagtg cttgttcagc cctaacttgg cttcgagcct
     9541 gtcggggttg ttatgaacaa tcaaccggta agcagcatct aaaatctgct ggccggatcg
     9601 gtagttttga gtcaggacca agggcttctt tggcttataa tgatcagtaa aatcaagaat
     9661 gttgctaatt tgagcacccc gaaatccata aattgcttga tcatcatccc ccaccgcaaa
     9721 aatattgccg tcttgaggaa taaatgttcg taataattca tcctgtaacc tgttggtatc
     9781 ctgatactcg tcaaccaaaa catagtggta ctgtttggtt aaacgctctg caagattgga
     9841 cttttgctgt aatatttgca acggaatggc tagttgatca ttaaaatcat acgttccagt
     9901 ttcagctttt aggctttcat acagggaata aagctgagaa agatcgtgtt gctcggtaat
     9961 gtcttgggga tgaagtccgg ctttgtctgg tttgagcgat gctgcatacg cggcataggc
    10021 tttagccgat accccggcat tctgaagccg cccaaggtac tccaccacgc cctccagaaa
    10081 ctcaaacata ctagcatgag ggccgtaata tgacaggcta acccgatcca aatgctgttg
    10141 caacaacagc gccttttggg tgtcattaat cagcccgccg tgagtcgagc gcccaatatg
    10201 gctggcatag gcgcctagta attcagcccc aaaggcatta aaggttacta ccggcacctg
    10261 atatgactcc cagccaatca ggtcataaag tcgatcagcc atctcgcgag cagctttttc
    10321 ggtaaaagta agcgccaaaa cttgatttgg cctggcggct ttggtttcaa tgagatgcgc
    10381 aatacgatga gtaataaccc gagttttccc ggtgccagcc ccggccacca ccagaatcgg
    10441 cccggcgcca taggtaacag cttggagttg ctcggggttc agttcgctca ggtccataca
    10501 gcccagtata tcaggcggtc tcggtgtcgc gctccatgcg cttgactggc gtaataccta
    10561 ataccttcga gtaggcttcc ataaaaacga tgtactgttt aataacatat agccggcggc
    10621 aagcctcggt tttgtctaaa tcaataatcc gctcgctttc atagaggtca tgaaataact
    10681 tcgccgcctg catcccaaaa aaggttaaat gatgaacttc gtagctatga gcgatgtcgg
    10741 cgactagacg cgggaatttg ctcatatgac gcaccagtgc cacttcatat tccgaaagcc
    10801 gctcaacggt gcttatgggt gttaaaccgc gctcggaagc ttgatccagg atggagttag
    10861 cccgggcgta ggaatacata acgtaaaaaa gcggattttt ttgggattgc tccttcgcaa
    10921 ggctcagatc aaagtccatg ccggtatcag ccgaacgcat caaggtcata aaccgtgcta
    10981 catcggtgcc aacctcatcg atcaactctt cgatggtgac gaactgacct aagcgcttac
    11041 tgatttttac ctctttacca gctttcaaca gacgaaacca ttgagccacc accacctcaa
    11101 gctgtctgtt tggatgcaag attgcgacgg cggctttaac cgagggaaac tggtcgacat
    11161 gatcagcgcc taagatctta atcgctgagt caaacgagcg gcggccgaat aaatcatcat
    11221 gaaaggcaat atccggtgct aaataggttg gatcaccggt acttttaatg agtacgcgag
    11281 cttcccgaga caagccgagc ttcgctgtat caagccataa agccccttcg tgatcataca
    11341 ccaagttctg cttacgcagc tgaccgatcg tttcctccag ctgaccgccc tcaaccagat
    11401 cgcgctcatt aaaccaacta tcaaaatgaa tgcccatctt atcgatcgcc gggcgaatcc
    11461 agcgctcgag tattgcctgg gtaatcagtt cctttagctc atcatcggat tttgcagcca
    11521 attcagcctt aaaatcgctg gcaagctcat gaatgtattc acccttatac tgcacagctt
    11581 cagctctgat caaccctgca gccaccttaa ccgactcaag tagcttagca atttgggttc
    11641 cggcattgtt gaaataatat tcacgggtta cagcgtagcc gctgtgagct aaaacatttg
    11701 ccaaggtgtc gccaatatag ccgccacgag catttccaat ggttgttggt ccagttggat
    11761 tagctgagat gaactcaacc tcaactcttc tcccctttcc tagctggctg ctgccaaatt
    11821 ccgacgtaat agacagcagt tggccgaccc aaaagtgatt atccatgcgc atattaataa
    11881 acccgttaac cgcttcagcc tcagcgatcg tattgcccgt atcggcagca atcgcagctg
    11941 ccagttccgt cgcgacttcc ccagggggct gattcaacct tttagccaat agaaaggctg
    12001 cattcgttga aaagtctcca aattttggct ctggataggt aataacgaga cttatatcat
    12061 ctatgctata tttgctgttt atggcgctac gaacagcatt ttggacatcg tcgatcatca
    12121 tgtgcctagt atagcaggca cttctgacct gtataatggc tgtatgaagc tattagccaa
    12181 aaaccggcgg gccacctatg actaccagat tgaagacaag ctggtggcgg ggttagtgct
    12241 gtctggtgca gaggtta
//
LOCUS       DASVGB010000123         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_178590, whole genome shotgun sequence.
ACCESSION   DASVGB010000123 DASVGB010000000
VERSION     DASVGB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178590"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VF272_03795"
     CDS             complement(<1..614)
                     /locus_tag="VF272_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260023.1"
                     /translation="MFRSIVSNLSFSPASTGQLTFYWRRLKGEHLTRQISMVMAGALV
                     FLQVATIIAPPDPATASSSNDIVYGGIGCDQHPKQTTLALYDKNASFRALLNRYGVYK
                     VDLENTTLGSLNSNNHAFKSLGRNPQFSASQGEQTVHGLDGHTYYERPLWRWGDNVVY
                     RALEGRRHGDNKYFAIMCNCGNLVITDSSPTSPPPPPPPPPPPPP"
     gene            783..2867
                     /gene="gyrB"
                     /locus_tag="VF272_03800"
     CDS             783..2867
                     /gene="gyrB"
                     /locus_tag="VF272_03800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX7260024.1"
                     /translation="MAVEQKKYGADQIQVLEGLDPVRKRPGMYIGGTGIEGLHHLIWE
                     VINNSIDEAMAGYGNTIEVTMLADGGVKVNDYGRGIPVEKHKATGKSSLETVLTVLHA
                     GGKFGGDGSGYKVSGGLHGVGISVVNALSTKVVVDVFRDGKHYSQEYSIGKPVGVLKV
                     IGPSERNGTTISFWPDGSIFESIEFNYQTVLDYLRHQAYLTKGIHVKAANEATGESYG
                     YYFEGGIRSYVEHLNRDKSILNQPPFYIERKVGDIVVELALQYTESFSENVRTFANNI
                     HNPEGGTHLTGFRSALTRVVNDYSRKNGLLKEKEDSLTGEDVREGMTAVISVKLPDPQ
                     FEGQTKAKLGSPEVRGVVEQVVNEWFSYYLEENPQEARKIVGKATLSARARLAARAAR
                     DTVIRKGALDGFTLASKLADCRTKDVNRSELYIVEGDSAGGSAKAGRDSEFQAILPLR
                     GKILNVERARLDRMLGNNEIKNLIVAMGTGIGDQFEIAKLRYGHVIIMTDADVDGAHI
                     RTLLLTFFYRHVPEIISGGHLYIARPPLYLVQAGRKKTYAYTEEERDAIIDRLIEEKA
                     AHKKAKKDEADAAEAAAAEQGGEFEQINDSASAPDPDDEELALSDEERNRLKMAGASI
                     QRYKGLGEMNADQLWDTTMNPENRIMLQVSIDDAEKADAIFNKLMGEEVLLRKNFIQT
                     HATVVSDDDLDF"
     gene            3045..>3357
                     /locus_tag="VF272_03805"
     CDS             3045..>3357
                     /locus_tag="VF272_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7260025.1"
                     /translation="MEDSYLKYAMSVIVSRALPDVRDGLKPVHRRILYVMNNMGLRYN
                     AKYRKSAAVIGDVMGGYHPHGDSAIYDAMVRMAQEFSMRYPLVDGQGNFGSIDGDPPA
                     AM"
BASE COUNT          878 a          747 c          913 g          819 t
ORIGIN      
        1 ggcggcggcg gtggtggtgg aggcggcgga ggcggagggc tcgttggaga gctgtcggtt
       61 atcaccagat tgccacagtt gcacataatc gcaaagtatt tattgtcacc gtggcgccgg
      121 ccttcgagtg cacggtagac aacattatcc ccccaacgcc aaagcggacg ctcgtaatag
      181 gtatggccat ctaacccgtg aaccgtttgc tctccttggc tggcgctaaa ttgagggtta
      241 cggcctaatg acttaaatgc atggttattg gagtttaaac tgccaagggt ggtattttcc
      301 aaatcaactt tatatacgcc atagcggttg agcagtgcgc gaaacgatgc atttttatcg
      361 tatagtgcga gcgttgtttg cttcgggtgc tggtcgcaac cgataccacc gtaaacgata
      421 tcgttagatg atgaggccgt ggccggatcg ggcggagcaa taatggtagc tacttgaaga
      481 aaaaccaacg caccagccat caccatggaa atttgacggg taagatgttc acccttgagc
      541 cgacgccagt aaaacgttag ctggccggta gaagccggac taaagcttag attagatacg
      601 atactccgaa acataaagcg gatataaact cctcgtcctt ctatttaagc attaccgcta
      661 tacctaccgc aagcttaatc ttatggatgt atggttgcct gatggtagct gaagcactat
      721 aatggtggta atagttgggt gagaaaatca ccttttttag ttgaggttta cgtttaaaat
      781 ttatggcagt tgagcagaaa aaatatggtg ctgaccagat acaggtgctt gaaggacttg
      841 atccggtacg taaacgtccc ggtatgtata tcggtgggac cggcattgaa ggccttcatc
      901 acctcatttg ggaagtaatt aataactcca tcgatgaagc catggctggt tacggcaata
      961 ctattgaggt gaccatgctg gctgatggcg gcgttaaggt gaatgactac ggacggggca
     1021 taccggtcga aaagcataag gctaccggga agagcagttt ggagaccgtt ctaaccgtat
     1081 tgcacgctgg cggcaagttc ggcggggacg gatccggcta taaagtttcc gggggtttgc
     1141 acggcgtcgg tattagcgtg gtaaacgcgc tctcaaccaa ggtagttgtt gatgtctttc
     1201 gagatggcaa gcactattct caagaatata gtattggaaa gccggttggt gtgcttaaag
     1261 tcataggtcc aagcgagcgc aacggtacaa ccatcagttt ttggccggac ggttccattt
     1321 ttgagtcaat tgagtttaat tatcaaaccg ttctcgatta tctgcgccat caagcctatc
     1381 ttacgaaagg catacacgta aaagctgcta atgaagctac tggtgagagc tacggctatt
     1441 attttgaagg cggtatcagg agctatgttg agcatttgaa ccgtgataaa agtatcctta
     1501 accagccgcc cttctatata gagcgcaaag tcggcgacat tgtggttgag ttggcgctgc
     1561 aatacaccga aagctttagc gagaacgtgc gtacctttgc aaacaacatt cacaatccgg
     1621 aagggggcac tcatttaacc ggtttccgtt ccgccctgac tcgggttgta aatgactact
     1681 cgcgtaaaaa tggcctgctt aaggaaaagg aagacagcct taccggcgag gatgtccgtg
     1741 aaggtatgac cgccgttatc tcggtgaagt tgccggatcc acagtttgaa ggtcaaacca
     1801 aggctaaact ggggagcccg gaggttcgcg gggtggttga acaggtggtc aatgaatggt
     1861 ttagctatta tctcgaagaa aacccgcaag aagcccgcaa gatcgtcggt aaggcgacac
     1921 taagtgcccg agcccgtctg gctgcccgag ctgcccgtga tacggttatt cgaaagggtg
     1981 ctctcgatgg ctttacactc gccagtaagc tggctgattg ccgcactaag gatgtgaatc
     2041 gcagcgaact ctatattgta gagggcgaca gtgccggcgg gtcggcaaag gccggtcgtg
     2101 acagtgagtt tcaggcgatt ttaccgctcc gggggaaaat tctaaatgtt gaacgtgccc
     2161 gtctcgatag aatgctcggt aataatgaaa ttaaaaactt gatcgtagcc atgggaaccg
     2221 gtattggcga tcagtttgag attgccaagc tgcgttatgg ccacgttatt atcatgaccg
     2281 atgccgatgt cgatggcgca cacattcgta cattgctgtt aaccttcttc tatcggcacg
     2341 ttccagagat catttccggg ggtcatcttt atattgctcg gccgccgtta tatcttgtcc
     2401 aggctggccg caagaagacc tatgcctaca ccgaggaaga gcgagatgct attattgacc
     2461 ggcttattga agaaaaagca gcgcataaaa aggcaaaaaa ggatgaagct gatgctgccg
     2521 aggcggctgc agcagagcag ggcggggagt ttgagcaaat caatgactca gcctcggctc
     2581 ctgatccaga cgatgaagag ctagccctca gcgacgaaga gcgcaaccgg cttaaaatgg
     2641 ctggagctag catccaacgg tacaagggtc taggcgagat gaatgccgat cagctctggg
     2701 ataccaccat gaaccccgaa aaccggatca tgctccaagt gagtattgat gacgccgaaa
     2761 aagccgatgc tattttcaac aagctaatgg gtgaagaggt acttctgcgg aagaacttca
     2821 ttcaaaccca tgctaccgtg gtttctgatg atgatttgga tttctaagcc gatggtaaag
     2881 cgttacatat gaaccccgaa gatataaaac ctgaggatat atatcccgaa gacgtaaacc
     2941 ccgaagagct gccggaatcg ggcgaagacc tgctgccttt aattgctgaa aaggtgcgcc
     3001 atgttggtgc cggtgagata cagaaccaaa gcctcgaatt tgaaatggag gacagttatc
     3061 tcaaatatgc tatgtccgtt attgtttctc gtgcacttcc tgatgttcgc gacgggctca
     3121 aacctgtaca ccgccgaatt ttatacgtaa tgaacaacat gggacttcgc tacaatgcca
     3181 aatatcgtaa aagcgctgct gtaatcggag atgttatggg ggggtatcat ccccatggcg
     3241 attcggcgat ttatgacgct atggttcgta tggcgcaaga attctcgatg cgttatccct
     3301 tagtcgatgg ccagggtaat tttggctcca ttgacggtga cccccccgcg gctatgc
//
LOCUS       DASVGB010000124         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_938461, whole genome shotgun sequence.
ACCESSION   DASVGB010000124 DASVGB010000000
VERSION     DASVGB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938461"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /gene="thrS"
                     /locus_tag="VF272_03810"
     CDS             <1..1313
                     /gene="thrS"
                     /locus_tag="VF272_03810"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7260026.1"
                     /translation="YGVAFTEKDELNNYLRQQAEAEKRDHRKVGPELGLFIMSELVGA
                     GLPLLLPKGETLKHTLREYMREKEEALGYQYVSTPVMAHEELYKRSGHAGFYDDSMYK
                     LTDDEDKVFYLKPMNCPHHHMIYERMAQSYRDLPLKLAEGDGIYRKELSGTLSGLIRV
                     RGPISQNDSHIYIEPQDLKTEFIKVLELFKAVYEEVNLTGYWYRLSLPDFDDPKAKFA
                     GDKQVWEDASQAIRDALEEFGAEYVEAEGEAAFYGPKLDVQIRNVTGHEDTIATSQVD
                     VLVPHRMGLTYIDQHGKAVEPIIIHRAILGSYERFMAFLIEHTAGNFPVWLAPEQVRL
                     ATVSDAPAIQERAREMRDQLKAAGVKVELDSTDESVGKKIRIATMQKVPYMIVVGEKE
                     VESANVSPRVRKDLGEGEPKLDFNEFVKQVQDEIKQRAIQSTLT"
     gene            1273..1629
                     /locus_tag="VF272_03815"
     CDS             1273..1629
                     /locus_tag="VF272_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016476995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX7260027.1"
                     /translation="MKSNSAPSNQPLPDRLPSEFAKAVYDLVAQIPQGRVMTYGQVAA
                     LCGSPRSARIVGQVAHWGPLDLPWQRVVNKQGGLAGGYTSGGREAHKRDLEADGVTVN
                     EKYQVDIKALIWWPPQ"
     gene            1626..2480
                     /gene="miaA"
                     /locus_tag="VF272_03820"
     CDS             1626..2480
                     /gene="miaA"
                     /locus_tag="VF272_03820"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7260028.1"
                     /translation="MTPRTLIAIVGPTASGKTELALQIAKMHNGEIICADSRTVYRGM
                     DLGTAKPTKAERASVPHHLLDAVDPGQVFSVADFQRLAARAIKGIQKRRRLPVVVGGS
                     GLYIDSLLYGFALRPLGTEQREDLSKRSDDELRWMLDDKGLVLNPSDRLNRRRLERAI
                     GSGLTVQQPRILPQNYHMIGLNPGIKLLEQRIAERTRQWFEKNILEETDALVTTFGLV
                     EPLNTPPYREIIENAVKDDDPAAVQDLINLHLRQLAKRQLTWFKRNLDIQWSASPEEA
                     IEIIKKLF"
     gene            2630..3502
                     /locus_tag="VF272_03825"
     CDS             2630..3502
                     /locus_tag="VF272_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7260029.1"
                     /translation="MSSIESGILIGLATAGGFGTADFLAAKASRQFGAVYTAFWAQAI
                     KVGLVATCFIALGWQLHITEVALLWAFLSGGITAIGLIAGFHALKLGPVGLASPFISG
                     YALIAALLSLSFLHESLRPMQLIAVVLLVAGAMMASMNIQTHRPHKGFWMKPAVLFAL
                     TAALGIGTGLFFLALLVREVGWQSSTFLQSCMMLAVMGLGMVLSKQPLFSQKRQPLSW
                     ALIGAASFSFIATILINFGFSSSLVAIVAPVASISPLVTVGLALYFFRERLSAYQLVG
                     AMVIIAALTLLAVR"
BASE COUNT          854 a          842 c          990 g          903 t
ORIGIN      
        1 tctatggcgt tgcatttacc gaaaaagacg agttgaacaa ctatctgcgt caacaggcgg
       61 aggctgaaaa gcgtgaccac cgaaaagtag gaccggagct agggcttttc attatgtcgg
      121 aattagtcgg cgcggggtta ccgttgctgc ttcctaaagg tgaaactctc aaacatacct
      181 tgcgcgaata catgcgagaa aaggaagaag ccctcggcta tcagtatgtg tctactcctg
      241 ttatggccca tgaagagttg tataaacgga gcggccatgc cggcttttat gacgacagca
      301 tgtataagct gaccgatgat gaggataagg ttttctacct taaacccatg aactgtccgc
      361 accaccatat gatttacgag cggatggcgc aaagctatcg tgacctgccg ttgaaactgg
      421 ccgaaggcga tggcatctac cgtaaagagc tgtcgggtac gctttccggc ctcattaggg
      481 tccgcggtcc aatcagccag aatgactccc atatttatat tgaaccgcaa gacttgaaga
      541 ctgagtttat taaggttttg gagctattca aggcagtcta tgaggaagtg aatcttaccg
      601 gctactggta ccgactgtcg ttaccggatt tcgacgatcc gaaggctaaa tttgccggag
      661 acaaacaggt atgggaagat gccagccagg caatccggga cgccctggag gagttcggcg
      721 ccgagtatgt tgaagctgaa ggggaggccg ctttttatgg tccgaagctg gatgtgcaaa
      781 tccgcaatgt gaccgggcat gaagatacta ttgccaccag ccaggttgat gtgttggtgc
      841 cgcatcgcat ggggctgacc tatatcgatc aacacgggaa agcggttgag ccgattatta
      901 ttcatcgcgc tatcctgggt tcatatgagc gctttatggc gttcttgatt gaacatacgg
      961 ccggaaattt ccccgtctgg ttagcgcccg aacaggtaag gttggctaca gtgagcgatg
     1021 cgccggcgat tcaggagcgg gcaagggaaa tgcgcgacca gctgaaagct gcaggagtta
     1081 aagtggagct tgatagcact gatgagtcgg ttgggaagaa aatccggatc gctaccatgc
     1141 agaaagtacc ctacatgatt gttgtggggg aaaaagaggt tgagagcgca aacgttagcc
     1201 cacgcgtccg caaggatctc ggcgaaggtg agcccaagct ggatttcaat gagttcgtta
     1261 agcaggtaca agatgaaatc aaacagcgcg ccatccaatc aacccttacc tgaccgcctg
     1321 ccaagcgaat tcgccaaggc cgtttatgat cttgtggctc agattcccca aggccgggta
     1381 atgacctacg gtcaagtcgc ggccctctgt ggctcgccgc gctctgcccg gatcgtcggc
     1441 caagtggcac attggggtcc gctagacttg ccttggcagc gcgtggtgaa taaacaaggc
     1501 ggactggctg gcggctatac ttcgggaggc cgcgaggctc ataagcgcga cctggaagct
     1561 gatggtgtaa cggtgaatga gaagtaccag gttgatatta aagcgctcat atggtggccg
     1621 ccgcagtgac ccccagaacc ttgatcgcaa ttgttgggcc aaccgctagc ggtaaaactg
     1681 agctggccct ccagattgcc aagatgcata atggcgagat tatctgcgca gactcccgta
     1741 ccgtctatcg cggcatggat ctgggcaccg ccaagccaac gaaagctgag cgcgcgtcag
     1801 ttcctcacca tctgcttgat gctgtggatc cgggccaagt attttcggtg gccgattttc
     1861 agcggcttgc ggctcgcgcc ataaagggta ttcaaaagcg gcgaaggctt ccggtggtgg
     1921 ttggcggttc tggtctttat attgactcgc tgctgtatgg gtttgcattg cggccgctcg
     1981 gaaccgaaca gcgggaggac ctatctaagc gctccgatga tgaacttcgc tggatgcttg
     2041 atgataaagg gctggtgctc aatccaagcg accgtctaaa ccgccgccgg ctggagcggg
     2101 cgatagggtc tggccttacc gtgcagcagc ccagaatttt acctcaaaac taccatatga
     2161 ttggcttaaa tccgggcatt aagcttctgg agcagcgcat cgcagagcgg actaggcagt
     2221 ggtttgaaaa aaatattctc gaagaaactg atgcgttagt cacaacattt ggccttgttg
     2281 agccgcttaa tacgccgccc tatcgtgaaa ttattgaaaa tgccgttaaa gatgatgatc
     2341 cagctgctgt tcaagaccta atcaacctcc atttacgtca attggccaag cgccaactaa
     2401 cctggtttaa gcgcaatctc gatattcagt ggagcgcttc accagaagaa gccatcgaaa
     2461 ttatcaagaa actgttttaa ttcatttatt tgtagctact cagttttgag tacgtaaata
     2521 ctcaaaaagt actcaaatcg gtgtacaaaa tgagtactta agtactcaga tgtgtgtgcc
     2581 gccagaaaaa tgaattagta cctcagattg ttgcttgtat aatgccccta tgtcttcgat
     2641 agaaagcgga atactaatcg gattggctac ggctggaggt tttggaaccg ccgattttct
     2701 cgcggctaag gcttcgcgtc agtttggagc ggtgtataca gcattttggg ctcaagcaat
     2761 taaggtcggg ctggttgcta catgctttat agcgctcggg tggcagctgc acataaccga
     2821 agtggctctg ctgtgggctt ttttgagcgg gggaattacg gcaataggcc taattgctgg
     2881 gttccatgcc cttaaattgg gtccggtggg cttggcaagt ccgtttataa gtggctatgc
     2941 cctgattgcc gcccttttgt ccctaagctt tctgcatgaa tccctaaggc ctatgcagtt
     3001 aattgcggtc gtactgttag ttgcaggcgc tatgatggct tcaatgaaca ttcaaaccca
     3061 tcggccacat aagggatttt ggatgaagcc cgcagtctta tttgccctta cggctgcact
     3121 aggcattggt actggattat tctttctggc acttttagtt cgagaggttg gttggcagag
     3181 ttcgacattc ctccagtcct gcatgatgct ggcagtgatg gggctgggga tggttctatc
     3241 gaagcaacca ttattctcgc aaaagcggca gcccttaagc tgggcactta tcggggcagc
     3301 cagcttttcg ttcatagcca cgattctgat taactttggt ttttcctcat ccttggtggc
     3361 aattgttgct ccggttgcgt caatttcgcc cctagtcacc gttggactag ccctatactt
     3421 tttcagagaa cgcctatcgg cctatcaact agtgggtgcg atggtgataa tcgccgcctt
     3481 aacgctgttg gcggtaaggt agaaaggttc ttatgtctgc accagaactg ggaataatta
     3541 caggcttagt tgctgctgtc ggcttcggag gcggtgactt ttttagcgc
//
LOCUS       DASVGB010000125         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1140599, whole genome shotgun sequence.
ACCESSION   DASVGB010000125 DASVGB010000000
VERSION     DASVGB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140599"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF272_03830"
     CDS             <1..205
                     /locus_tag="VF272_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260030.1"
                     /translation="AVKQAKDTLLYAVIGIVVAILAYAIVNFVAGALKSNSSTPPPAA
                     SPPGLTGTASPGPAPALTGTSGP"
     gene            277..693
                     /locus_tag="VF272_03835"
     CDS             277..693
                     /locus_tag="VF272_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX7260031.1"
                     /translation="MNKLLINDKVLKASIFAAGLVPMSAHAITAETCSDPNSAANPIS
                     SGAQCSQAKGTSGDLFGATGIFHTIANILIFIVGAIAVIMLIIGGLRYVISQGNKEHV
                     SSAKDTILYSVIGIIVAILAFAIVNFVTGSLTPTAP"
     gene            complement(756..1187)
                     /locus_tag="VF272_03840"
     CDS             complement(756..1187)
                     /locus_tag="VF272_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7260032.1"
                     /translation="MARKNPTNDETVLENEFLNDEETDQNWLEEEQEVEGQLAVDVFQ
                     TKENVVIKAPIAGVDPKQLEVAIAEDVVTIRGERVDDSVVERENYYVQECYWGAFSRS
                     IIMPVSVNADKAEASLKNGVLSISIPKVIQDKIKKIKVIAG"
     gene            1364..1969
                     /locus_tag="VF272_03845"
     CDS             1364..1969
                     /locus_tag="VF272_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7260033.1"
                     /translation="MLQQLARFLAPPCCLSCDVEGSLVCPSCWEKVFQPAEIGCNGLA
                     NVIAAGPYASVMRELVARLKYKHQREAAAVFARALLPRLEPKRFEYNSVTAVPVVTAR
                     LRQRGYNQAELIAKELARSLALPYRRLLVRRRVTQQVGKSRAERLEHVGGAFVAKPNV
                     GGLRVLLIDDVLTTGATLAECAGVLHRAGANEITGAVAAWD"
     gene            complement(1981..2073)
                     /locus_tag="VF272_03850"
     tRNA            complement(1981..2073)
                     /locus_tag="VF272_03850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2037..2039),aa:Ser,seq:gct)
BASE COUNT          465 a          546 c          499 g          573 t
ORIGIN      
        1 ggctgttaag caggctaagg acacattgct ttacgccgtt atcggtattg tcgttgcgat
       61 tttagcctac gcgattgtta actttgtggc aggagccttg aagtctaatt cctccactcc
      121 tcctccggct gccagtccac ctggtcttac cggcaccgca tccccaggtc ccgctccggc
      181 ccttaccggc acatccggcc cttaaattag ccatcagttg caatctgtga tagtattgca
      241 gcaaaataag cgtaacaact taaaaggaat ttaccagtga acaaactact tataaacgat
      301 aaggtgctca aggccagcat ttttgccgct ggtttagtac caatgtctgc tcatgcgatt
      361 accgcagaaa catgcagcga tccgaattct gcggcaaatc cgattagctc tggcgctcag
      421 tgttcccagg ccaaaggcac cagcggcgac ctgttcggtg caaccggaat ttttcatacc
      481 attgctaaca tcttaatctt cattgtcggt gctatcgccg ttatcatgtt gattatcggc
      541 ggcttgcgct atgtaatttc tcagggtaat aaagagcatg tatcgtcagc caaagacacc
      601 attctttact cggtaattgg tatcattgtg gcgattctgg catttgccat cgtcaacttc
      661 gttaccggat ccttgacccc cactgctcca taggcgtttc aatagctaac aaaaaagacc
      721 cgaattcggg tcttttttga tgactggcct ccggactatc cggcgataac tttgattttc
      781 ttgatcttgt cttgaattac ctttggaatg ctgatgctca acacgccgtt tttcaagctg
      841 gcctctgcct tatcggcatt cacgcttacc ggcataatga ttgagcggct aaaagcgccc
      901 cagtaacatt cctgtacata gtagttttcg cgctcaacaa ccgaatcatc tacacgttct
      961 ccgcgaatcg ttaccacgtc ttcggcaatc gcaacttcta gctgcttagg gtcaactccg
     1021 gcaatcggtg ccttaatgac cacattttcc ttggtctgaa atacatcgac tgcaagctgt
     1081 ccttcaactt cttgttcttc ctcgagccag ttctgatcag tttcttcgtc gtttagaaac
     1141 tcattctcta atacggtctc atcattggtt gggttctttc gtgccacgct atgtctcctt
     1201 aaaagctcct acctcagtaa ggcaaatgtt gctcacttgc atcggccttt tgcctgatta
     1261 ttccacacct gaagcccaaa agcaatacag gtatgggttg ttagcggata gcttaagatt
     1321 ttgaaaataa gtatctctta tagtagcagg atgaaaggag tccatgctac agcaacttgc
     1381 caggttttta gctccaccat gctgcttaag ctgtgatgtt gaaggcagtt tagtgtgccc
     1441 aagctgttgg gagaaggtgt ttcaaccggc tgagattggc tgcaacggat tagcaaatgt
     1501 aatcgccgcc ggtccgtacg cgtcggtgat gcgtgagctt gttgcgcgcc tgaagtataa
     1561 gcatcagcgc gaggccgcag cagtctttgc gcgcgccctt ctcccccgtc ttgagcctaa
     1621 gcggtttgaa tacaatagtg ttacagctgt tccggtggtt acggcccgtt tgcgccaacg
     1681 cgggtacaat caggccgagc tcattgctaa agagcttgca cgcagcctag cactccccta
     1741 ccgccggctt ttggtgcgcc gtcgtgtcac tcaacaagtc ggtaagtcgc gcgccgagcg
     1801 tctagagcat gtgggcggtg cgtttgtagc caagcccaat gtaggcggtt tgagggtgct
     1861 gctaattgat gatgttttaa caacaggtgc cacgttggct gagtgcgccg gtgttctcca
     1921 tagagcgggg gcgaacgaaa ttaccggcgc cgtggcagcc tgggattaaa aaataagtgc
     1981 tggcggagag ggagagattc gaactctcgc ggggacttac gtccccctac aggtttagca
     2041 aacccgcccc ttcagccact tgggtacctc tccataggcg gtt
//
LOCUS       DASVGB010000126        12255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_379950, whole genome shotgun sequence.
ACCESSION   DASVGB010000126 DASVGB010000000
VERSION     DASVGB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 12255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12255
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379950"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1855)
                     /locus_tag="VF272_03855"
     CDS             complement(2..1855)
                     /locus_tag="VF272_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260034.1"
                     /translation="MAEEDQPGPDDPSVDRYTHPLSEEGSGEPGPAVGGTAMDVDALG
                     GFDAAVQRMQEESERRVPVEQQRAEFYDAMAGVFGNPRGGEDVLDVIGRGLQEKTITL
                     DEVTEKILENPTITLDGVDRAHAEYRREVDGHLLGNGGDGDQDAIDRAHAKAVILTAV
                     QRGMVLAKAEGWDATPERRAWTHPEPVPAAPDAAVAPPPDMAPAPPSPPDAAPLPDDA
                     SPDHAALAHRSRLPIKGGLRGLNPFRRKRRQADEEYAEPTTTPYVRPEGFDPSTRMSA
                     TAQKGALDSFVKANKAGLGHSKEDLKRSADDIDPARREAFVEENQSGLQEASLRLAAL
                     AAYDSSSLMMKTSNEDMKQARDKYETAFKAELMRDLESVDIEFANRFLEDQGDQDRDH
                     LNDEQKILRAQVRVALFRLVDSQLKRSLHGARALGLRNRKTTHFVEGFGNDRRIQQAI
                     SGAMRGNARELHTTDPEPTGSERRPGGRLVPTNLRQARDLPALQAHELYTNPEKERGK
                     RTAAQLSARAAFSRDEQQTETDSRTLANLMQAEIEEIVGQLGSIQETGTPDDIMSSIA
                     DIITPRLAQQLQSEAEQNVYDRKKSRRARSRRLVRDWRDRHAARSSGGIIN"
     gene            2009..2347
                     /locus_tag="VF272_03860"
     CDS             2009..2347
                     /locus_tag="VF272_03860"
                     /inference="COORDINATES: protein motif:HMM:NF039821.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HEX7260035.1"
                     /translation="MPNDIVTFVAWIIDQRPEAGKIPEAEKAIMRKDLESQLDQMVNT
                     ALLAEMSPEQLDEFSDLLDEDSEENVQYFIEQAVPHSKEIVTGVLADFAKMYLGESHA
                     RTSDTPSSES"
     gene            2310..5300
                     /locus_tag="VF272_03865"
     CDS             2310..5300
                     /locus_tag="VF272_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260036.1"
                     /translation="MPEHQTPHPQNPEPPDTGVEPQNEDGPEAEFSFSTARIDQRDIR
                     NQAYEHADEALQREQAEMLEGKGMKKLAGRIHNVWRQTITRTARRQNLMRDAEAELIN
                     AQSIHARELGDPEAARETQQHDMEALIARTTEGYLRSGEGEEVTESSNPAVEAKVKEF
                     IRIYADDDAAFDQLEPALEGVRQAMQETHTDTIAGFSVEQLVELAANVKEKVTTERTL
                     DDMLGQIQFYEAQVRAGVRTELQRTKVEKILDWADQHPRLVISAKTAALAVTAAAVLS
                     KGFTSAAGKIAGTVVPGTVGATMASIHEAKALKGEKRHLERASAKGKPVASEGQERRQ
                     ELAGSLYEKVSASSLVDRMSRLTEGAPEDLDAAQTRTLLNIVAEITMRERLSLEKGTD
                     LISFSSARDVETERFDLARLRAKAAHYAGERMPLLTQAERAATGIGTHRNVGEFVEAW
                     IDQSISSDQITDGIEAKDKIYRKIRNGRVSKAMVKGALTGVTLGLIAQETAGTARSDY
                     QGITDGALAASPAMAENQTFLRNLLDGSGVWDGPDVHSSSTGITGEGEQASDQTGAAP
                     PPAAVKPQEFDHSRPWFQDHAYDGNDHFERIHEGEGIGISNGFRLVHEIQGQDSNAGY
                     LSLKDSYGNSFVEGLAQGPQGLTPDSIDKLHSYGIETHFNPAGDGGRGRLDIIVPPEM
                     NVNAPETVPEAPESKASSEPHQPSGFDSNPRAKDYIPPAIASPTPTGRRELERRPTTA
                     STTASVATDAPAPEASTPSPETGATSDRWWEGLNLNVPERPPAGAEVEPDTEVEPEPA
                     PEAEALVEPEPEPAVEPEEPPVVEPDPDVEPEPEPEPAVPEREPQSDYAEQRRQITSA
                     SGRGLHDLLQLAERFNADGLVSYSSSSTEPTSVEPAADKNWTDTYIDFVEHLASSIQR
                     VEKAEFNTKADNYATQLAEATTHAELRAIVDDMVDSDIARIENADEGENYNVEIINDK
                     MSGGKREQLETIIRRTQEAIAGAS"
     gene            complement(5304..5630)
                     /gene="rbfA"
                     /locus_tag="VF272_03870"
     CDS             complement(5304..5630)
                     /gene="rbfA"
                     /locus_tag="VF272_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7260037.1"
                     /translation="MSQRTEKVSSLIKQIIATELTHLPESAYLTVTNVEVSPDLRQAT
                     VWVGVFSKSDEEATNLFKSVETAQNRLQAAVARGLTTKFTPKLNFKPDSSGAYAEDIT
                     RLIKGL"
     gene            complement(5641..7476)
                     /gene="infB"
                     /locus_tag="VF272_03875"
     CDS             complement(5641..7476)
                     /gene="infB"
                     /locus_tag="VF272_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7260038.1"
                     /translation="MRRPISAPHTTNQTKEVKMAETETVSEIQSVEIAPVVGVGAFAE
                     KLGIPVTSVIAELMKNGVMATINEQIDFDTAAIIGADLGFEVIVEKAEPTSAATPKVK
                     LPQGEGTVRPPVVAVMGHVDHGKTSLLDAIRDTDVAKAEAGGITQHIGAYQVKRNDRW
                     ITFLDTPGHEAFSAIRAHGARLTDVAIIVVAADDGVKPQTKEAVRYAQEAGNHIVIAI
                     NKTDKTGSDPNRVRQELSEQLELVPEEWGGSTVMVDVSAKSGDGIEKLLDMVLLVADV
                     EDLRARPAGPSEGVIIETHLETGRGAVATVLVQNGVLKPGDFVVAGDTYAKVRSLEDW
                     RGKRIKRADPGMPAVITGLKSAPAFGDAYYAVENERQARDRSVANQRQASIKSMTKVK
                     KIGIDELSSAIAAGRVQELNVVVKADVQGSLESLLDSLNALGNDEVAMKVVSSGIGDI
                     NESDVAFAATSGALLLGFNVSLTTKVKQLATHEKVQIRLYRVIYELLDDMRSGLSQML
                     KPEIIETVIGELKVLGVFRTTKTLVICGGEVLSGKASAPSQVRIKRGKEVVGAGTLTG
                     VQKEQNKVSEAVEGEQCGLNINTTTAIEVNDRLEFFTTEERQRSL"
     gene            complement(7654..8793)
                     /locus_tag="VF272_03880"
     CDS             complement(7654..8793)
                     /locus_tag="VF272_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7260039.1"
                     /translation="MPAKTANKASTDVTMEELLAEHQVTGMTAGDLVEGIVIASDKHE
                     IWLDLGERGTGLVVGREIERASDINPGDQISASVIEPEADEGYALLSLKKVAKEKGWE
                     SLQEKFESGEVFAITPFDANKGGLLVEIDGIRGFLPVSQLSAENYPRVSGADKDEILH
                     RLTNLVNKPLNVRVLDVNRRDNKLIVSEKAARREDTESKVSQLKTGQVVNGLVTGVVD
                     FGIFVNVDGIEGLVHISEIAWDRVDNPGKYVKVGEKVEAKIIAIDRDKLSLSIKQMQE
                     DPWIGAVKKLKVGAEVEGKVTRITPFGAFVQVTPVIEALVHISELSDDHVEDPTKLLK
                     VGESKQFRIIAIDPDAHKLSLSLKAPKKTTLEKPVAEKPVKAAKE"
     gene            complement(8934..9428)
                     /locus_tag="VF272_03885"
     CDS             complement(8934..9428)
                     /locus_tag="VF272_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260040.1"
                     /translation="MESIKKDFPQFTFVPGNFFMWSPHSKSIHYDTRRKGTNEGKIAL
                     VHEVGHALLGHRLYKYDMELIKMEMDAWDMVRRLAPDYNLYIDEEHIARCVATYDAWL
                     TKRATCPDCQNFSLQKGRDEYGCFACGSIWQVNWRKDRRVTRRVLERYENPQLSHTEF
                     LASS"
     gene            complement(9502..10335)
                     /locus_tag="VF272_03890"
     CDS             complement(9502..10335)
                     /locus_tag="VF272_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7260041.1"
                     /translation="MHIGVDIGGSKILVVAGTSGHAILRQAKIKTPESGKAGMAEIIR
                     MIEEVAGNDVIRAIAIATASPIDRKKGRILESAPNMHGWRGTELVKPIKERFSVPIYL
                     ENDAAAAALTESHIGAGRNHPYVLYVTISTGIGTALVINGQPYRGGAGSLEAGHMIID
                     PNGPQCGCGGRGHFEAMVAGPAIRKRYGKYGYEITDPEIWDEIAGFIALGFYNLIHAY
                     APSVIVIGGGVGVHYEKFHPFLVKHLEKLHPLYPIPPIVPAKHIETAVGYGALILASQ
                     L"
     gene            10609..11037
                     /locus_tag="VF272_03895"
     CDS             10609..11037
                     /locus_tag="VF272_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260042.1"
                     /translation="MNRNVLTTIGGLILVGVVVVGTFLYGDQQRQKQLSSDNTAATQQ
                     EREKAQTAAPTPAPAPTAQPTPAPRATTTKPSPAASTAKPRTQPKTTAVQPAAGSSSA
                     PAPSQTPKTGSGLVNIVAVSLMAVVYIAYRRSRNFAPYQV"
     gene            complement(11102..11305)
                     /locus_tag="VF272_03900"
     CDS             complement(11102..11305)
                     /locus_tag="VF272_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX7260043.1"
                     /translation="MAGTARGGRKAAATNKMRYGANFYESIGALGGRKSRGGGFAKNP
                     DLAREAGRRGGMASRRRKASASS"
     gene            11542..12033
                     /locus_tag="VF272_03905"
     CDS             11542..12033
                     /locus_tag="VF272_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX7260044.1"
                     /translation="MVQLTLILDNLRSAYNIGAILRTCDAAGVHQVFACGTTPYPKLK
                     NDSRDPVISGRNTREIAKTALGAEKTVMVEHFNDTASAINYCRAHKWTLIGLEQSSES
                     KNLFEYSPPQNIALIVGNEVEGLNAEALGACDIIAEIPQRGTKESLNVSVATGVALYT
                     LLF"
BASE COUNT         2965 a         3347 c         3026 g         2917 t
ORIGIN      
        1 cttagttgat tattccccca ctgcttctag cagcatgcct gtcccgccaa tccctcacta
       61 atctgcggct tcgagcgcgg cggcttttct tccggtcgta gacattctgt tcggcttctg
      121 attgaagctg ttgcgctaaa cgaggagtga tgatatctgc tatagagctc atgatatcat
      181 ctggcgttcc cgtttcctgt atcgatccaa gctggccaac tatttcttca atttctgcct
      241 gcatcaggtt ggccaatgtt cttgagtcgg tttctgtctg ttgctcatcg cggctaaaag
      301 cagcccgtgc agacaattga gctgccgtac gcttgccgcg ctctttttcg gggttcgtat
      361 agagttcatg cgcttgcaag gcgggcagat ctcgggcttg ccgtaagtta gttggcacga
      421 gccggccgcc tggacggcgt tcggatccgg tcggctcagg atcggtcgta tggagctcgc
      481 gcgcatttcc gcgcattgcg cccgatatcg cttgttggat tcgcctatca ttaccaaagc
      541 cttcaacaaa atgagtggtt tttcgattgc gaagccctag ggcacgtgcg ccatgtagcg
      601 aacgcttgag ctggctatca acaagccgga aaagcgcaac tcttacctgg gctcttaata
      661 ttttttgctc atcgtttagg tgatctcgat cttggtcgcc ctggtcctct aggaagcggt
      721 tagcgaattc aatgtccacc gattcaagat ctctcatgag ctctgcttta aaggcagttt
      781 catatttgtc ccgcgcttgc ttcatgtctt cgttcgaggt tttcatcatc aaactagagc
      841 tatcgtacgc agcgagtgct gctagccgca agcttgcttc ttgcaaaccc gactgatttt
      901 cttccacaaa agcttctcgt ctcgcaggat cgatatcgtc agcgctacgt ttcaaatctt
      961 ctttgctatg accaagcccg gctttgttgg ccttcacaaa tgaatcaagc gccccttttt
     1021 gcgccgttgc actcatccga gtactggggt caaaaccttc cggtctgacg tagggcgttg
     1081 tggttggttc tgcatactct tcatcagcct gccggcgctt gcgccgaaaa gggttgagtc
     1141 cacgtaagcc gcctttaatg gggagccgtg atctatgagc cagggcggcg tggtctggag
     1201 atgcgtcatc gggaagcggc gcggcgtctg gcggagatgg aggcgccggc gccatgtctg
     1261 gcggtggagc aacggccgca tccggcgcag ctggcaccgg ttcaggatgc gtccatgccc
     1321 gacgttcggg agtagcgtcc cagccttcgg ccttagcaag caccatcccc ctttgaaccg
     1381 ctgtaagaat aaccgccttt gcatgcgccc tgtcaatcgc atcctgatcg ccatccccgc
     1441 cattgccaag taagtggcca tcaacctcgc ggcgatattc agcgtgagct ctatcaaccc
     1501 catcgagggt tatagttgga ttttccagaa ttttttcggt aacttcatct agggtaatgg
     1561 ttttttcctg caggccgcgg ccgatcacat caagcacatc ctctcccccc cgcggattgc
     1621 caaaaacccc ggccattgca tcatagaatt cggctcgctg ttgctcgact ggcacgcggc
     1681 gttcactttc ttcctgcatt cgttggaccg cagcatcgaa gccgcctagg gcatcaacat
     1741 ccatcgccgt accgcctacg gcaggacctg gctcaccgga cccttcctcg ctcaggggat
     1801 gagtatagcg atctacagag ggatcatcag gaccaggttg gtcttcttca gccataatgc
     1861 ttatccttat ctatggatat tgtagcagtt gaacaccttt aagcaaccgg caaatatgat
     1921 cggttttgaa agcacccgcc gtcaggctta ccagattgac ttatcattaa aacccattac
     1981 acttatgctt aagataagga acattacgat gcccaacgat atagtgacat ttgttgcctg
     2041 gattattgat caaaggccgg aagccggcaa gattccggaa gctgaaaaag caattatgcg
     2101 taaagatctt gagtcacagc tggatcaaat ggtaaatacg gcattgctgg ctgaaatgtc
     2161 tccggagcag cttgatgaat tcagcgatct gctggacgaa gattcagaag agaacgttca
     2221 atattttatt gagcaagccg tcccccacag caaagaaatt gtgactggcg tactcgctga
     2281 ttttgccaaa atgtacttag gagagagtca tgcccgaaca tcagacaccc catcctcaga
     2341 atcctgagcc tccagatact ggggttgagc cgcaaaacga agacggccct gaagccgaat
     2401 tttcgtttag taccgcacgc atagatcagc gggatattcg caatcaggca tatgaacacg
     2461 ccgatgaagc actgcagcgc gaacaagcgg aaatgttgga aggcaaaggc atgaagaaac
     2521 ttgccggccg gatacataat gtctggcgcc aaacgattac ccgcaccgcc cgccgccaga
     2581 atcttatgag agacgccgaa gcggagctga tcaatgctca aagcattcac gctcgtgaac
     2641 tcggcgatcc tgaagccgca cgagagaccc agcaacatga tatggaggca ttaattgctc
     2701 ggaccaccga gggctatcta cgttcaggag aaggcgaaga ggttacggaa agttcaaacc
     2761 ccgcggttga ggctaaggtg aaggagttta ttcggatata tgctgacgat gatgccgctt
     2821 tcgaccagct tgagccggca cttgaaggcg tgcgccaggc aatgcaggag acgcataccg
     2881 acacgattgc cggattcagc gtggagcagc tggttgagct ggcggccaat gttaaggaaa
     2941 aggtaacaac cgaacgtaca ttagatgata tgcttggcca aattcagttt tacgaagctc
     3001 aagtgcgcgc cggcgtgcgt accgaactgc aacgcaccaa ggttgaaaaa atcctcgatt
     3061 gggcagacca gcacccccgc cttgttatct cggcaaagac agccgctctc gcggttacag
     3121 cagcagcggt attgtcgaaa ggcttcacca gtgccgccgg aaaaattgcc ggtaccgtgg
     3181 taccgggtac cgttggtgcc acaatggcca gtattcacga agccaaggcg ctgaagggtg
     3241 aaaagcggca cttggagcga gccagtgcta aaggtaaacc ggttgctagt gaaggccagg
     3301 agcgccgaca ggaattagca ggcagcctct atgaaaaggt cagcgccagc agcttggttg
     3361 atcgaatgtc cagactcacc gaaggagcac cggaagacct tgacgccgca caaacccgca
     3421 ccttactgaa cattgtggct gaaattacta tgcgtgagcg tcttagcctt gaaaaaggga
     3481 ccgatttgat tagtttcagc agcgcacgtg acgttgaaac cgaacgcttt gatttagccc
     3541 ggctgcgagc taaggctgcc cattatgccg gtgagcgtat gccgctgcta acccaagccg
     3601 aacgagccgc aaccgggatt ggcactcacc gcaatgttgg cgagtttgtc gaagcatgga
     3661 ttgatcaaag catttcctcc gaccagatta ccgatggcat cgaagccaag gataaaatat
     3721 accgtaaaat ccgcaatggc cgcgtaagta aggcaatggt taaaggtgcc ttaaccgggg
     3781 ttacgttggg ccttattgcg caggaaacgg ctggcactgc acgatccgac tatcaaggta
     3841 taaccgatgg tgcgctggca gcctcccccg ctatggctga aaatcaaacc tttttacgga
     3901 atcttctgga tggcagcggc gtatgggatg gaccggatgt tcattcttca agtaccggca
     3961 taaccggcga gggggaacaa gcatccgatc aaaccggagc cgctcccccg cctgcagctg
     4021 ttaaacctca agaatttgat cactcccggc cttggtttca agaccatgcc tatgacggca
     4081 acgaccattt tgagcgcatt cacgaaggtg aaggcattgg catctccaac ggcttccgtc
     4141 tcgttcacga aatccagggc caggacagta atgccggata tctcagctta aaagattcct
     4201 acggtaattc atttgttgaa ggactcgcgc agggaccgca gggcttaacg cctgactcca
     4261 tcgacaaact acactcatac ggcattgaaa ctcatttcaa tcctgccggc gacggcggtc
     4321 gcggccgttt agatattatt gttccgccgg aaatgaatgt gaacgccccg gagacagtac
     4381 cggaagcgcc tgaaagtaaa gcatcaagtg aaccgcatca accgtccggg tttgattcaa
     4441 atccgcgagc caaagattac ataccgccgg caatagcatc accaacaccc accgggcgac
     4501 gcgagctgga acgcaggcca accaccgcta gcacaacagc aagtgtagcc accgacgcac
     4561 cggcacctga agcgtctaca ccgagccctg aaaccggcgc aacgagtgat aggtggtggg
     4621 aaggcttaaa tctgaacgta ccggaacggc ctccagctgg agctgaagta gagcctgaca
     4681 ccgaagttga gcctgaacct gcgcctgaag ctgaagctct agttgaaccg gagccggaac
     4741 ccgctgttga gccggaagaa ccgccggtcg tagaacctga tcctgatgtc gaaccagaac
     4801 ccgagcctga gccggctgta cctgaaaggg agccgcaatc tgactacgcc gagcagcgac
     4861 gtcaaatcac ttcggcaagc ggaagaggct tacatgattt gcttcagcta gctgaacgtt
     4921 ttaatgctga tggacttgta agctattcat caagttccac cgagccaaca tcagtagagc
     4981 ctgcggccga taaaaactgg accgatacat acatagattt tgtcgagcac cttgcaagct
     5041 caattcaaag agttgaaaaa gctgagttca atacaaaggc tgacaattat gcaactcagc
     5101 ttgccgaagc gaccacccat gcagaattac gtgcaatagt tgatgatatg gtggacagcg
     5161 atattgcaag aatcgaaaat gctgacgaag gtgaaaatta caacgtcgaa atcattaatg
     5221 ataaaatgag cggcggcaaa agggagcagt tagaaactat aattaggcgg actcaagagg
     5281 caattgccgg agccagctaa atcctacaac ccctttatca gccgggtgat gtcttcggca
     5341 taggcgccgc tggaatcagg cttgaaattg agcttggggg tgaacttggt ggtaagtccc
     5401 cgcgcaactg ccgcctgaag gcgattttga gcggtttcaa cgcttttaaa gagattagtt
     5461 gcctcttcgt cgctctttga gaatacaccg acccagacgg tcgcttggcg caaatcaggc
     5521 gaaacctcga cattagtaac cgttaaatag gcagactcag gcaagtgcgt aagttccgtg
     5581 gctataattt gcttgattaa gctggaaact ttttcggttc tttgagacat gatttacccc
     5641 ctagaggctg cgctggcgtt cttcggtggt aaagaactct aggcggtcat taacctctat
     5701 ggctgtagtg gtgttgatat tcaagccgca ctgttcgcct tcaaccgcct cggagacctt
     5761 gttttgctct ttttggaccc cagttagggt gccggctcca acaacctctt tgccgcgctt
     5821 aatccgcacc tgactgggcg cgcttgcctt accgctcagc acctcgccgc cacaaataac
     5881 caaggttttg gttgtcctaa agacacccaa aaccttaagc tcgccgatga ccgtttcaat
     5941 gatttccggt ttaagcatct gacttaaacc actgcgcata tcatcaagca gctcataaat
     6001 taccctataa aggcggattt gaaccttttc atgcgttgcc agttgtttaa ccttggtcgt
     6061 aagcgacaca ttgaatccga gcagcaaggc tccggaagta gccgcaaagg caacatccga
     6121 ctcgttgata tcgccaatcc cgctggaaac caccttcatc gctacttcat cattaccaag
     6181 tgcatttagg ctatctaaca gagactcaag tgaaccctgc acatcagcct tcactacaac
     6241 atttagctcc tgaactcgcc cggcggcgat cgcgctcgac agctcatcaa tgccaatttt
     6301 cttcactttg gtcatgcttt taatcgaggc ttggcgctga ttagcaaccg agcggtcgcg
     6361 ggcttggcgt tcgttttcta cggcatagta ggcatcaccg aaagccgggg cagactttaa
     6421 tccggtgatt actgccggca tgccggggtc agctcgctta atacgcttgc ctcgccaatc
     6481 ttccaaactc cggactttgg cataggtatc gcctgcgact acaaaatcac ctggctttag
     6541 cacgccgttt tgaaccagta ccgtagccac cgctccgcgg ccggtttcaa gatgggtttc
     6601 aataataacg ccctcgctgg gaccggctgg ccgcgcccgc aaatcctcaa catctgccac
     6661 caaaagcacc atatcaagca acttttcaat accgtcaccg cttttagctg agacgtctac
     6721 catgaccgtg ctgccgcccc actcttccgg caccagctcc aactgctcgc tcagctcttg
     6781 gcgcacccgg ttaggatcag agccggtttt atcggtttta ttaatagcta tgacaatgtg
     6841 atttccggct tcttgggcat aacggacggc ttccttagtt tgcggcttca ccccatcgtc
     6901 agccgccacg acaataatgg cgacatcggt taggcgggca ccatgggcgc gaatggccga
     6961 aaaagcctca tgccccggcg tgtccaagaa agtgatccaa cgatcattac gcttaacctg
     7021 ataagcacca atgtgttggg taatgcctcc ggcctcagcc ttggctacgt cggtatcacg
     7081 aatggcatca agcaagctag tcttgccgtg gtcaacatga cccataacag ccaccaccgg
     7141 cggacgaacc gtgccttcac cttgcggcag tttcactttg ggtgtcgcgg cggaagtcgg
     7201 ctcagctttt tcgactatta cctcaaatcc taaatcggct ccaataatgg ccgccgtatc
     7261 gaaatcgatt tgctcattga tggtcgccat aacgccgttt ttcattaatt cggcaattac
     7321 gctggtaacc gggatgccca gtttttcggc aaaagcaccc acaccaacaa ctggtgctat
     7381 ttcaacagac tggatttctg atactgtttc ggtctcggcc atttttacct ctttcgtttg
     7441 attggtggta tgcggcgcgc taattggacg gcgcattcct cgctgcagct gtagattccg
     7501 cggccggcac ggcttcggcc tttatccgcc acaaggccgc tatcctgcat aacccagcgt
     7561 gtcagtgccg actgggggcg tcgggtccgg caaacccggc acatgcgcat cggaaccaat
     7621 gcgccactcc gcggcaccgg cgttcccact gccctattcc ttagcggctt taacgggctt
     7681 ttccgctacg ggtttttcta aagtggtctt tttaggtgct tttaagctta ggctcagttt
     7741 gtgagcatcg ggatcaatcg caataatccg gaactgttta ctttccccta cctttaaaag
     7801 ctttgtggga tcttcaacat ggtcgtcgct cagctcggaa atatgaacta aagcctcaat
     7861 gaccggcgtt acctgaacga aagctccaaa cggtgtaatg cgggtaactt taccctcaac
     7921 ttcagcacca actttcaact tcttaacggc accgatccag ggatcttcct gcatctgctt
     7981 aatacttagt gaaagcttat cgcgatcaat cgcaataatt ttagcttcaa ccttttcgcc
     8041 taccttgaca tacttgccgg ggttatcaac gcggtcccag gcaatttctg agatatgaac
     8101 cagcccttcg atgccatcga cgtttacgaa gatcccgaag tcgactaccc cggtaaccaa
     8161 gccgttgacc acttgaccgg ttttgagctg agaaaccttg ctctcggtgt cttcgcggcg
     8221 ggctgccttt tcagaaacaa tcagcttatt atcgcggcgg ttaacatcaa gcactcgaac
     8281 gttaagcggc ttgttcacca agttagtcaa acggtgcaaa atttcgtctt tgtcggcacc
     8341 ggaaacccgg gggtaatttt ccgcactgag ttgactaacc gggaggaatc cgcggatacc
     8401 atcaatctca accaacagtc cgcctttatt ggcatcgaac ggggtgatag caaacacttc
     8461 gccggattca aatttttctt gaagcgattc ccaacccttt tccttggcta ctttcttcag
     8521 acttaacagc gcatagcctt catcagcctc aggctcaatt acgctggcgc taatttggtc
     8581 gccggggttg atgtcgctag cgcgttcaat ttcgcggcca acaactaatc cggtcccccg
     8641 ttctccaaga tccagccaaa tttcatgttt atcagaagca attacgattc cttctaccaa
     8701 atcgccggca gtcataccgg taacttgatg ttcggctagt aactcctcca tagtaacgtc
     8761 ggttgacgct ttgtttgcag ttttcgcggg cacgcaggcc tccttataca aaagatttca
     8821 tcaccgcacc tcacagcacg ggaagatgat taggtttatt gtacctgatt tatggcttgg
     8881 ggtaaagcag ttgcaggctg tttgagcctt gcgctggcgg atatgaataa tagctagctg
     8941 ctggccaaga actcagtatg gctaagctgc ggattctcgt aacgttccaa aacgcgccgg
     9001 gttacccgcc tatctttgcg ccagttcacc tgccaaattg agccgcaggc aaaacagcca
     9061 tattcatcac gacccttttg aagactaaag ttttgacagt cgggacacgt agcccgtttg
     9121 gtcagccacg catcataggt agctacacag cgtgcaatat gctcttcgtc gatatacagg
     9181 ttatagtctg gggccagccg ccggaccatg tcccaggcgt ccatttccat tttgataagt
     9241 tccatatcgt atttgtataa acggtgcccc aagagggcgt gcccgacttc gtgcaccaag
     9301 gctatcttgc cctcattggt gcctttgcgg cgggtatcgt aatggatcga tttggaatga
     9361 ggcgaccaca taaaaaagtt tcccggcaca aatgtaaatt gcggaaaatc tttcttgata
     9421 ctttccaaat aaccctcccc ggcctagctt actgaacact atactactcc cgccgggaac
     9481 ccccggcaag catgagcaat attacagctg agaggccaaa ataagtgccc cgtacccgac
     9541 ggccgtttca atatgcttgg ccggaacaat cggcggtatc ggataaagag gatgcagttt
     9601 ttccagatgt ttaaccaaga aaggatgaaa cttttcatag tgaacgccaa cgcccccgcc
     9661 aattacgatc accgaaggcg cataggcatg aatcaaattg taaaagccta gggcaatgaa
     9721 cccggcgatt tcatcccaga tttcagggtc ggttatttcg tagccgtact tcccatagcg
     9781 tttacgaatt gccggcccgg caaccattgc ctcaaaatgt ccgcgcccgc cacagccgca
     9841 ttgcggaccg ttcggatcaa taatcatgtg gccggcctcc aaactgccgg caccgccccg
     9901 gtacggctgc ccgttaatga cgagggccgt tccaataccg gtacttatgg ttacgtacaa
     9961 aacataggga tgattacggc cggcacctat gtgtgattcg gtgagcgcag cagcagcggc
    10021 atcattttca agataaatcg gaaccgaaaa ccgctcttta atcggcttta ccagttcggt
    10081 accgcgccag ccatgcatgt taggtgcgct ctcgagaatg cgcccttttt tcctatcgat
    10141 tgggctggct gtagcaatgg cgatcgcccg gattacatcg tttcccgcga cttcttcaat
    10201 catccgaata atttcagcca tgccagcctt accggattca ggggttttga ttttggcttg
    10261 gcgaaggatt gcatgcccgg atgtaccggc aaccaccagg atctttgagc caccaatgtc
    10321 gacgccgata tgcatggaag tagtataacg cctgaaccac atatttcaat gattgcagcg
    10381 ttttgattga cataactcaa gacttatgtt attatcaagc actaatggca ttggtgaacg
    10441 tcagatgggg cgtcagctgc aaaggttaat atgagtgctc acctccttgt attgccgacg
    10501 cgtgacgtcc catctgagtg cagcagtgct tgcagattgg gacctagctg gtatactggc
    10561 gcgtaagagg gtgcgttaaa cagttaatgc acacggggaa ccaagcacat gaatcgaaac
    10621 gttttaacca ccattggcgg gctcatccta gtcggagttg tcgtagttgg cacctttttg
    10681 tatggagatc agcaacgcca gaagcagcta agcagcgaca ataccgccgc aacccagcag
    10741 gaacgagaga aagctcaaac cgctgcgccg acgccggcac cagcacctac ggctcagcca
    10801 acacctgctc ctagggctac taccaccaag ccaagcccgg cagcttcgac cgctaagccc
    10861 cggactcagc caaaaacgac ggctgtgcaa ccagctgccg gaagctcatc tgccccggcc
    10921 cctagtcaga ccccgaagac cggttctggc ctcgtaaata ttgtggccgt cagccttatg
    10981 gctgtggtgt atattgccta ccgccgcagc cgtaattttg cgccgtacca ggtataattc
    11041 atcaagatgc gcactaaaaa agacccttac cagggtcttt tttagaataa atcaatggtg
    11101 cttagctaga tgctgaggct ttgcggcggc gagatgccat cccgccacgg cgacctgcct
    11161 cgcgagccag gtcggggttt tttgcaaaac cacctccgcg actcttgcga ccccccaggg
    11221 caccgattga ctcataaaag tttgcaccgt agcgcatctt gttggttgca gcggccttgc
    11281 ggcctccgcg ggctgttcct gccatgagtt aatctccttt aattccttta ggtatattgt
    11341 gccttagggg cacagaaacc gctttagtgt ttcccacaaa ctgcttacgg gggccgtaag
    11401 aaaagtttgt cagaagcgac ataagcggct tcacctccta agtatagtag cacaatgcgc
    11461 ttacgctgtc aacgcttaaa tggactgctt ttgtatgcgt tgcggctcat ttaattgagt
    11521 acgttataat tgaacaaact gatggttcaa cttacgctta ttttagacaa cttgcgcagc
    11581 gcctataaca ttggcgccat actgcgcacc tgtgatgccg ccggagtaca tcaagtgttc
    11641 gcctgtggaa ccactcccta ccctaagcta aaaaatgact ctcgcgaccc ggtaatctct
    11701 ggccgcaaca cccgcgaaat tgccaaaact gccttgggtg ccgagaaaac ggttatggtt
    11761 gaacacttta atgatacagc ctcagcgatc aactactgcc gggcccataa atggacacta
    11821 attgggctcg agcagtcttc agaatcaaaa aatcttttcg aatactcccc gcctcaaaat
    11881 attgctctta ttgtcggaaa tgaagttgaa ggtttaaatg ccgaagcgct aggcgcttgc
    11941 gatatcattg cggagattcc tcaacgcggc actaaggaat ccttgaatgt tagcgtggca
    12001 accggtgttg cattatatac attactattt tagataaata cacttcgtgg agctgccgcg
    12061 ccggaacgcg gcagctccac tttagtcctg catgttttgc cgctagaaaa ccttgtcgat
    12121 gacgaaggcc gcgagcggca caaccatgcc gacaacgatg atgaaagccc ggaagcaagt
    12181 ctgcgcctgg cgagacgcca accagggccg cccactcgcc tgccacccat tcacaccttc
    12241 tggcacagca tctcc
//
LOCUS       DASVGB010000127         6361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_950543, whole genome shotgun sequence.
ACCESSION   DASVGB010000127 DASVGB010000000
VERSION     DASVGB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950543"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VF272_03910"
     CDS             <1..1511
                     /locus_tag="VF272_03910"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7260045.1"
                     /translation="KARGLSVNIMKCDPYLNTDAGTLNPVEHGEVFVTKDGAETDLDL
                     GHYERFLDAELTRSSSVMNGQIYAQLIADERSGRYLGKTVQIIPHVTDAIQDRIEAAG
                     KGFDIHIVEIGGTVGDYESLAFLDAIRQFRRRVGAENVLNAHVVYLPYLEASKELKTK
                     PAQNTVRDLRAAGVPTDIIFARAEREVSEHVLTKISLFCDLDKEAIVALPTAKSVYQV
                     PLTLESSGIADYVCKRFGIDADKADISAWQHLNKTIFNNDKPPVKIGVVAKYMDHEDT
                     YMSVTEAIKAASWAHGRQPEIVWVDAEGVEKEGPDGLLEGLDGIIVPGGFGSRGVEGK
                     INAAAWARKHKVPYLGLCLGMQVAVIAVAREVLNDPLANTTEIDTETKHPVIALMDEQ
                     RRVVDKGGTMRLGNYPCKLADGSKAREAYGTGKIEERHRHRYEFNNEYRDQLAEAGLK
                     IVGTSPDDKLVEIIELEDHPFFVASQFHPEFTSRPARPNQLFDAFVKAIVTE"
     gene            complement(1562..1834)
                     /gene="rpmA"
                     /locus_tag="VF272_03915"
     CDS             complement(1562..1834)
                     /gene="rpmA"
                     /locus_tag="VF272_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7260046.1"
                     /translation="MSHTKAGGSSKNIHDSAGKRLGVKLFGGEAVRGGNIIVRQRGTR
                     MEPGTGTALGKDQTIYATKSGRVSFTQKQVTKFTGQKVRRTFVTVD"
     gene            complement(1834..2961)
                     /locus_tag="VF272_03920"
     CDS             complement(1834..2961)
                     /locus_tag="VF272_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260047.1"
                     /translation="MHYLAILGRQPEFGLVELESLLGSDSVHPFGKSTAIIDRELDLN
                     RLGGTQKLGRILYRGPTRDLNEAPISLDELPIREGKTSFGLSYYGASITQRYVMAAGL
                     TLKKRLRSKGSMRFVAPQTGTHLTAAQVKFNGLTRGGFELIVATRKNEMIIARTEQIQ
                     DIDWYAVRDYQRPTRSASVGMLPPKLAQILVNTTSAPLVYDPFTGTGVILQEALLLNR
                     AARGSDLSEAMVTAAKENLTWLNGQRPDLPTWSIELVDARQLRLPDEPLAIVSEGYLG
                     PNMGKRPTLAEVNRLRRELGDLYAESLTNWAKQLPSGAEVSITAPMWLTAKGWHKLDI
                     IDRLSDLGYNLESFRHVDSRSLDYHRPNQIVGRQLLILRKQ"
     gene            3111..4292
                     /gene="xseA"
                     /locus_tag="VF272_03925"
     CDS             3111..4292
                     /gene="xseA"
                     /locus_tag="VF272_03925"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7260048.1"
                     /translation="MQPPIDQIIGVKAFLGVLNETLNFAFPSVIVEGEVSSFKVNQGK
                     WVFFDLKDEDATLPCFMPLYQLKVPLEDGMKVRATGVPKVTIWGKFSFTIKSIALAGE
                     GELKRAFQLLKAKLEKEGLFAAERKRRLPTIPRTIGLITSGQSAAYADFMKILQSRWG
                     GLDVQLADIQVQGEAAPNQIIQAINYFNQLAAPVDVLVIIRGGGSLEDLQAFNTEDVA
                     RAIAGSRTPTLVGVGHEVDVSLADYAADIRAATPTDAARLVVPDRNQVLQDIAHRLTY
                     LNQAITGQIAARHHLLDRHASRLERFVRLPQARLQTLQHRLLVAQQRLTSESRHTLTS
                     FERLLNSYNPAATLQRGYAIVRHKGTVLKQAEQVSAGASLMIQLAAGKLTTKVEDEHA
                     Q"
     gene            4276..4533
                     /gene="xseB"
                     /locus_tag="VF272_03930"
     CDS             4276..4533
                     /gene="xseB"
                     /locus_tag="VF272_03930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7260049.1"
                     /translation="MSTPSKPSDFTQKLSELEAITEWFESGEVDLNQALTKFERGMEL
                     TEELKKQLQDVENRVQKIRAKFDGTAPHQDAEVLEPPLLPE"
     gene            4530..5015
                     /locus_tag="VF272_03935"
     CDS             4530..5015
                     /locus_tag="VF272_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260050.1"
                     /translation="MNEWFWLAAAVVACFGVGAIMGAPFLPIRNPDIEAALDLAKLKP
                     GQTIIDLGSGDGRILLAAARRGALAIGYEINPLLFAWSLMRTWPHRRRITIHLMSYWH
                     SKLPPADIIYTFLITRYTAKLDKKLKAELSRPTAVVSYIFELPREPVAQTYNTWLYRY
                     P"
     gene            5086..5739
                     /locus_tag="VF272_03940"
     CDS             5086..5739
                     /locus_tag="VF272_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260051.1"
                     /translation="MKERGEINLHLVLTIFLGFGMVLFGILAIIAYRDNTYIHTNLNI
                     LNAKAAQEAADEQKKLDDAAYIKANQLPFRTYTAEPVNGGFQLQIPKNWSLYAGNNTT
                     ALTQLDLASSPDVVVVNSGPNAVNTHAFHLQLRKASAASINKTFEARLKKKTLTSRGV
                     IVSGIQGTWFEGTIDDQRHNGVVVVLPVRDKTMVISTETRNYLDEFNKILSTAKIIP"
     gene            5803..>6361
                     /locus_tag="VF272_03945"
     CDS             5803..>6361
                     /locus_tag="VF272_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7260052.1"
                     /translation="MAQRKSSFHDAAVQAPVAVELGVDQVWESMQDGLILVGRDNQIQ
                     YVNPAASRLVGLNDSAIGQSLTTVINELQDYGFPDAIVTIEGDAAGAQERALLGHTSS
                     LVVRLKRNTSQTALEALLGPYYDADRTITGILISLHDQTAVYAEQEKMRIVQQSHSIG
                     VIMLDTHNMVTSVSSRVPQLNEALSN"
BASE COUNT         1585 a         1559 c         1662 g         1555 t
ORIGIN      
        1 tgaaagcccg cgggctctcc gttaacatca tgaagtgcga tccttatctg aataccgatg
       61 ccggaacctt aaatccagtt gaacacggcg aggtctttgt tacgaaagac ggggcagaaa
      121 ccgatctcga ccttgggcat tatgagcgct ttttagatgc ggaactaacc cgcagtagct
      181 cggtgatgaa cggccaaatt tacgcgcagc ttattgccga cgaacgcagc ggccgctatc
      241 ttggtaaaac cgtccaaatt attccgcatg tgaccgatgc tattcaggac cggattgaag
      301 cggccggcaa gggctttgat attcatattg tagaaattgg cggaaccgtg ggcgactatg
      361 aaagcctggc ttttcttgat gccattcgcc agttccgtcg ccgcgttggc gcagaaaacg
      421 tactgaacgc ccatgtggtt tatctgccat accttgaagc cagcaaggaa ctgaaaacca
      481 agccggcgca aaacacggtg cgcgatctac gggccgctgg tgttcccacc gacattattt
      541 ttgcccgagc cgagcgcgaa gtcagcgagc acgtgctgac caagatcagc ctgttctgtg
      601 atcttgataa agaggcgatt gtggccctac ctaccgcgaa gagcgtgtat caggtaccac
      661 ttaccttgga atccagcggt attgccgact acgtttgcaa gcgtttcggg attgatgccg
      721 ataaggctga tatttccgcc tggcagcatt taaataaaac cattttcaat aacgataagc
      781 ctccggtaaa gattggtgtt gttgccaagt atatggacca tgaggatacc tacatgagcg
      841 ttaccgaggc gattaaagcg gctagttggg cccacgggcg ccaacccgag attgtatggg
      901 tagatgcgga gggcgtggaa aaggagggcc cagacgggct gcttgaaggg ctggacggca
      961 ttatcgtgcc gggtggattc ggttcccgcg gggtggaagg caagatcaat gcggcggcct
     1021 gggcgcgcaa acacaaagtg ccttacttag ggttgtgctt aggtatgcag gtggcagtga
     1081 ttgccgtggc ccgtgaggtt ttgaatgatc cattggccaa cactaccgag attgatacgg
     1141 aaaccaagca tccggtaatt gcgctcatgg atgaacagcg gcgggtggtt gataagggcg
     1201 gtactatgcg gttggggaat tatccttgca agctagcaga tggatcaaaa gcccgcgagg
     1261 cttacggtac gggcaagatt gaagagcgcc atcgtcatcg ctatgagttt aataatgagt
     1321 accgtgatca gttggcggaa gcaggcttaa aaatagttgg cacttcaccc gatgataaac
     1381 tggtcgaaat tattgaactt gaagatcatc ccttctttgt agccagccag tttcatcctg
     1441 agtttacctc gaggcctgct cggcctaacc agttgtttga cgcatttgtg aaagcgatcg
     1501 ttacggagta gcgattgtag cgcaatagcc gggtgcctcg gtaggaggag tacccgccga
     1561 attagtcgac ggtaacaaac gtccggcgga ctttttggcc ggtaaacttg gtaacctgtt
     1621 tttgggtaaa ggaaacgcga ccgcttttag tggcataaat ggtttggtct ttccctaagg
     1681 ccgtgcccgt gccgggttcc atgcgggtgc cgcgctgacg tacgataata ttgccgccac
     1741 gaactgcttc gccgccaaac agcttaacgc caaggcgctt tcccgctgaa tcatgaatat
     1801 ttttagatga accaccggct tttgtatgcg acattactgc ttcctcaaaa ttaataactg
     1861 gcgtcccaca atttgattgg ggcgatgata gtccaaacta cggctatcaa catgcctaaa
     1921 actttccaaa ttatagccta aatcagataa gcggtcaatg atatccagct tatgccaacc
     1981 cttagcggtt aaccacatgg gtgcagtaat actaacttcc gctccgcttg ggagttgttt
     2041 agcccagttt gttaggcttt cagcgtagag atcaccaagc tcccggcgta gtctatttac
     2101 ttcggcaaga gtaggtcgct tgcccatatt cggccctaaa taaccttcgc tgacaatcgc
     2161 cagcggctcg tccggcaatc gcagctggcg agcgtcaacc agctcaatcg accaggttgg
     2221 caggtcggga cgctgcccgt tcagccaggt taaattctcc ttagccgcgg ttaccattgc
     2281 ttccgataga tctgatccgc gggctgctcg attcagtagc agggcctcct gcaaaataac
     2341 gccggtccct gtaaagggat cgtataccag tggcgctgag gtggtattca ccaggatctg
     2401 agccagttta ggtggcagca tgcccaccga cgcgctgcgg gttggacgct gataatcgcg
     2461 gactgcgtac cagtcgatat cttgaatttg ctcggttcgg gcgatgatca tttcgttctt
     2521 gcgggtcgcc acgatcagct caaagccgcc tcgggtgagc ccattaaact ttacctgcgc
     2581 agccgttaaa tgcgtaccgg tttgaggtgc tacaaaccgc attgagccct ttgagcgcaa
     2641 acgtttcttg agcgttaagc cggcagccat cacatagcgt tgagtaatgc tcgcgccgta
     2701 ataacttaaa ccaaaactgg ttttaccctc gcgaatcggc agttcatcta aggagatcgg
     2761 cgcttcattg aggtcgcggg ttggaccgcg gtagagaatc cgccccagtt tttgagtgcc
     2821 gcccaaacga ttcaaatcca gctctcggtc tataattgca gtcgactttc caaatgggtg
     2881 aacgctgtcc gagcccagta gcgattccag ttcaaccagc ccaaactccg gctgtcttcc
     2941 caatatggcc aagtaatgca tgggctttat tgtactctag tgccgcttgt cgtgtagcta
     3001 catggctact tttctgttaa tgcacatttt taacacaatg ctgcaaatgt gcattaacag
     3061 aaaacaccac aaaagtagta ccaagcagcc ttggcatcta taatggactc atgcagccgc
     3121 caatcgatca aattattggt gtaaaagctt ttctgggcgt cttaaatgaa acgctcaact
     3181 ttgcgtttcc ttcggttatt gtggaaggcg aggtatccag ttttaaggtg aatcaaggca
     3241 aatgggtatt tttcgacctg aaagatgaag acgccacctt gccctgcttt atgccccttt
     3301 atcagttaaa ggtaccgctg gaagatggca tgaaggttcg ggcgaccggt gtaccaaaag
     3361 ttactatttg gggtaagttt agctttacca tcaagtcaat tgcattggcg ggggaagggg
     3421 agttaaagcg ggcatttcaa ctgctgaagg ctaagcttga aaaagaggga ctgtttgcgg
     3481 ccgaacgcaa acgccgttta ccaacgatcc ctcgtaccat cggcctgata acctctggtc
     3541 agtcggctgc ttatgctgac ttcatgaaaa ttctgcaatc gcgatggggc gggcttgatg
     3601 ttcagctggc tgatattcaa gtacagggcg aagcagcgcc taaccagatt attcaagcaa
     3661 tcaactattt caatcaactt gctgcgcccg ttgatgtgct ggttattatc cgcggcggcg
     3721 gcagcttgga agatctccag gcattcaata cagaggacgt tgcccgggca attgccggca
     3781 gccgtacccc gacattggtg ggagtagggc atgaggttga tgtgagcttg gccgactacg
     3841 ctgccgacat ccgggctgcc acccccaccg atgcggcgcg gttggtggtg cccgatcgta
     3901 accaggtact acaagatatt gctcacagac tgacatattt gaaccaagct ataaccggac
     3961 agattgccgc gcggcaccat cttctcgata ggcacgccag taggctggag cggtttgtgc
     4021 gcttacccca agccagactg caaacgctgc aacatcggct cctagtggct caacagcgat
     4081 taacttccga aagccgccat acactgacca gttttgaacg gctgctgaac agttataatc
     4141 cggctgcgac gctgcagcgc ggctatgcca ttgtgcggca taagggcacg gttctaaaac
     4201 aagccgaaca ggtgtcggcc ggggcatcac tcatgataca attggccgca ggaaaactaa
     4261 cgactaaggt agaagatgag cacgcccagt aaaccgtcag attttactca aaaactcagc
     4321 gaactcgaag caattaccga atggtttgaa tccggcgagg tcgacttaaa ccaagccctg
     4381 accaagtttg agcggggaat ggagctgaca gaagaactta aaaagcagtt gcaggatgtg
     4441 gagaatcgag tgcaaaaaat ccgtgcgaaa tttgacggaa cagcaccgca tcaagacgct
     4501 gaagttttag agccgccact tctgcccgaa tgaatgagtg gttttggctg gctgcagccg
     4561 tagtcgcgtg ttttggcgtg ggtgcaatta tgggtgcacc attcttgccg atacgtaatc
     4621 ctgatattga ggcagcgctg gatctggcga aactcaaacc cggtcaaacc attattgatt
     4681 taggctccgg cgatggacgc attctgctag cggccgcccg ccgcggcgca ctagcaattg
     4741 gctatgaaat taatcccttg ctttttgcat ggtcattgat gcggacgtgg ccgcaccgtc
     4801 ggcgtattac cattcacttg atgagttact ggcatagtaa actcccccca gctgacataa
     4861 tttatacgtt tttaattacg cgctacaccg ccaagctgga caagaaactg aaggctgaat
     4921 tatcacgtcc gaccgcggta gtaagctata ttttcgaact gccgcgcgag ccggttgccc
     4981 aaacctataa cacctggctt tatcgctacc cttaaaccga ggccagcatc gcatagctac
     5041 aaaagttgcg ttcatccctg gcggtgtggt ataaccataa gcagaatgaa agagcgcggt
     5101 gaaattaatc tccatctagt tctcaccata ttcctaggat ttggaatggt gctttttggc
     5161 atattggcga ttattgctta ccgcgacaac acctacatac ataccaatct gaatattctt
     5221 aatgcaaagg cggctcagga agctgccgac gagcaaaaga aacttgatga tgctgcctac
     5281 attaaagcca accaactgcc gttccgtacg tataccgccg agccggttaa tgggggattt
     5341 caacttcaga tacctaagaa ctggagtctc tatgcgggca ataacaccac tgcgctgact
     5401 cagcttgacc tggcttcaag tcctgatgta gtagtggtaa atagtggacc gaatgcggtc
     5461 aacactcatg cttttcattt gcagctgcgt aaagcctcgg ccgcatccat caataaaact
     5521 tttgaagcaa ggttaaaaaa gaagacactg acctcccggg gagttattgt ttcgggaatt
     5581 caaggaacgt ggtttgaagg taccattgat gaccagcggc ataatggggt tgttgtggtc
     5641 ctcccggtgc gcgataagac catggtgata tctaccgaaa cccgcaatta ccttgatgaa
     5701 tttaataaaa ttttaagcac ggccaagatc attccataat cccttatgct tgagtcgcca
     5761 tctgatagca tgctacctta ggactactaa gtgtgggtga atatggcgca acgcaaatct
     5821 agttttcatg atgccgcagt gcaggcgccg gttgcggttg agttaggtgt agatcaagtt
     5881 tgggagagta tgcaggatgg gctgattttg gttggccggg ataaccaaat ccagtacgta
     5941 aacccggctg cctctcgtct tgttgggctc aatgactcgg caattggcca gtcgctgaca
     6001 acggtcatta atgagcttca agactatggc tttcccgacg ccatcgttac catagagggt
     6061 gatgccgccg gtgcccagga gagggcactt cttggtcata ccagtagttt ggttgtgcgc
     6121 ttgaagcgta acacatcgca aacagcgctt gaagcgctgc ttggaccata ttatgacgct
     6181 gatcgtacta ttactggtat attgattagc ctgcatgatc aaaccgctgt ctatgccgaa
     6241 caagaaaaaa tgcgtattgt gcaacagagc catagtattg gtgtaatcat gctcgatact
     6301 cataatatgg ttacctcggt tagctcgcgt gtaccccaac tcaatgaagc attgagcaac
     6361 c
//
LOCUS       DASVGB010000128         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1212612, whole genome shotgun sequence.
ACCESSION   DASVGB010000128 DASVGB010000000
VERSION     DASVGB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212612"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..564
                     /locus_tag="VF272_03950"
     CDS             148..564
                     /locus_tag="VF272_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260053.1"
                     /translation="MNSGLLAYRRFITFLLKSPRRAFALIPAVLVSAFMIGFGFSSVA
                     FPESGPSGKVGVVPPQVALHLLSLVPAAYAAATAPLPCASTCNALDPNSYSTTSDFYA
                     ANAAGVDFPFILQGDSNVGYINVNSPRKSVTKRFDQ"
     gene            561..1097
                     /locus_tag="VF272_03955"
     CDS             561..1097
                     /locus_tag="VF272_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7260054.1"
                     /translation="MRRGFNQAGDTLVEVVFAIAIISITLVSAYNVANNSYRIGVQAR
                     ERIEAASVAQGQAELLRAWRDQNTVNMAPNQGVVPSPAGGFKVTMSPTGVITSTACGP
                     TCLVGPGGRYQLRVTAAEVGTVFPSTSGPDGVKYTIRVEWPRIGGTGNENTEVTLTLA
                     DRRLDRTNIECDEVEKCS"
     gene            1088..1780
                     /locus_tag="VF272_03960"
     CDS             1088..1780
                     /locus_tag="VF272_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7260055.1"
                     /translation="MFVKRSNQKGFTLVETVLAMAIFTFVLLILVGALTGLYRIYRAT
                     AGIRDTQQQARTVSEEITRATRNASYVIPKIDTASGNNAICLYRPASRVSNILRGPSE
                     VFSLEGTTTKKIVWRKLDAADLALDGSTVCDPNLYANPRDLTSPEVSVMLFYPTAASL
                     VAPATLKTMLNFELRIASTRALASEINTSVAFDPANPENTGPVCDDGLSYCSMTGISS
                     GVQARSQIAGGD"
     gene            1782..2858
                     /locus_tag="VF272_03965"
     CDS             1782..2858
                     /locus_tag="VF272_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260056.1"
                     /translation="MIKTQSGGASIIAVGFIAILILILTVSITRLMVGELRHALDMEN
                     NMNAYYQAEGGVEEAILALRHLSSVDPDALLTTPNQNCDVDGVPTNFGVAGGTSIAAN
                     ANITCRRIRTVSNFVDSTLLIGESAHFDVSRKNFHAIEIEWDSESLNKQSPDTDFSNL
                     QQGHPAPPFLEVTRIEYPNTGAVTNINPTAISIKSLFLAPTKGVPDCFAGTGTTNYQD
                     CEGDDGTLAPGGRVKTKCSIKPIYRCKVVFTHFMASTPPATRTVLRLRPYFGAMQYTM
                     KLYDSTGKRVDIGLDKTIIDVTARIGGSYRRIEQEVTLLPKPLEGIEAVFGDDKVCKK
                     FEIINTGSGEFLREDSIPPDCLLD"
     gene            complement(2867..>3788)
                     /gene="xerA"
                     /locus_tag="VF272_03970"
     CDS             complement(2867..>3788)
                     /gene="xerA"
                     /locus_tag="VF272_03970"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEX7260057.1"
                     /translation="ISSTSLSDLVTDYLEYLEIEQNRSQKTIANYDRYLQRLVAFSDD
                     VAIHNLTPDVVRKWRVWLNRLTDSSGEPIGKSTQNYHLIALRSFLKYLAKRDIEALTS
                     DKIELANVKRPQVTFLEVEEVKRLLEIITIETPVGLRDRAIIELLFSGGLRVSELVSL
                     NREHINLDRREFSIRGKGQKDRLIFISPDAALWIDRYLKSRPDNFKPLFVHYGGSQDE
                     LDDGAYTRLTARSVQRLVSRYAKLAGITKRVTPHTLRHSFATDLLSNGADLRSVQGLL
                     GHANVSTTQIYTHLTDPQLRKVHEQFHRRS"
BASE COUNT          908 a          959 c          989 g          932 t
ORIGIN      
        1 cgatgggaaa actaccagcg tggttccccg gcaatcggtt gtcggggtga aaggcacgta
       61 tgcggtaagc gctgctgcaa ccaccggcaa tgcgtttgat gcgaagaact cacagctgct
      121 actggaaggt tcattaaggg ggttacgatg aattctggat tattagccta tcgccggttt
      181 ataacattcc tattgaagag tccgcggcgg gcattcgcgc tcattccggc ggtgctagta
      241 agtgctttta tgattggatt cggcttcagt tctgtggcat tcccagaaag cggaccttcc
      301 ggcaaagtgg gtgtagtacc gccccaagtt gccctccatt tgctcagttt ggtacccgca
      361 gcctacgccg ccgcaacggc ccctctgccc tgtgcctcca catgcaatgc cctcgatccc
      421 aatagttatt ccacaacctc ggatttttat gcggccaacg ctgcaggggt agattttcct
      481 tttatacttc agggcgattc aaacgttgga tacattaacg ttaattcgcc gcggaaaagc
      541 gttacgaaaa gatttgatca atgaggcgtg gtttcaacca agccggagat acgctggtag
      601 aggttgtctt tgcgattgcc attatctcca taacccttgt aagcgcctat aatgtggcaa
      661 ataacagcta tcgcattggg gttcaggcgc gggagcggat tgaagcagcc agcgtcgctc
      721 aagggcaagc cgaattgctg agggcatgga gagatcaaaa tacggtgaat atggcgccca
      781 accagggcgt agtaccaagt cccgctgggg gatttaaggt tacgatgagt cctaccggtg
      841 ttataacttc aactgcttgc ggcccgacct gcttagtagg gcctggcgga cgctaccagc
      901 ttagagttac tgccgccgag gttggtaccg tctttccatc aacctccggc cctgatggcg
      961 ttaagtacac tatacgagtg gagtggccgc gcataggcgg taccgggaat gaaaataccg
     1021 aagttacctt aacgctcgcg gaccgtcgcc tcgataggac caatattgag tgcgacgagg
     1081 tagaaaaatg ttcgtaaagc gttcaaatca aaagggtttt actcttgtag aaactgtgct
     1141 tgcaatggca atatttacct ttgtattgct gatccttgtc ggggccttaa ccggcttgta
     1201 ccgcatttac cgggccactg ccggcattcg cgacacccag cagcaagcta ggactgttag
     1261 cgaagaaatt acccgggcaa ctcgcaacgc ttcgtatgtg ataccaaaaa tagacaccgc
     1321 ctccggcaat aacgccatct gcctctatcg gccggcttct agggtaagta atattctgcg
     1381 cggacccagt gaggttttct cgctggaagg taccaccacc aaaaagattg tttggcggaa
     1441 actggacgct gccgatcttg cgttggatgg ctccacggtt tgcgatccga acctttacgc
     1501 taatccgcgg gatttaacct cgcctgaagt ctcggtaatg cttttttatc caacggctgc
     1561 cagcctagta gcgcccgcta ctcttaaaac catgttgaat tttgagctgc gaattgcttc
     1621 aacccgggcc ttggccagcg aaataaacac ctcggtggcg tttgatcccg caaatcccga
     1681 aaacaccggc ccggtttgcg atgacggctt atcatactgc tcaatgacag gcatatctag
     1741 cggcgtacag gcacggtcgc aaatagcggg gggagactaa catgataaag acgcaatccg
     1801 gcggcgcctc tataatagcg gttggtttta ttgccatcct gatcctcatt cttacggtgt
     1861 cgattacccg cttgatggtg ggagagctaa ggcacgcgct tgacatggag aacaatatga
     1921 atgcctatta ccaagccgaa ggcggtgtag aggaggccat tctggcattg cgccacctga
     1981 gttcggtaga tccagatgcg ctgctaacaa cgcccaacca gaactgcgat gtggacggcg
     2041 tacccaccaa ttttggcgtc gctgggggaa cctcgattgc tgctaacgcc aatattacct
     2101 gccgtcgtat ccgcacggta tctaattttg ttgatagcac ccttttgatt ggcgagtccg
     2161 cgcattttga tgtaagccgt aaaaatttcc atgcgatcga aattgaatgg gacagtgaaa
     2221 gccttaataa gcaaagcccg gatacggatt ttagtaacct gcagcaaggc cacccggcgc
     2281 cgccttttct agaagttacc cggatcgaat atccgaatac cggggccgtg accaatatta
     2341 atcctactgc tattagcata aaatcgctgt tcttggctcc taccaaaggg gtgcctgatt
     2401 gcttcgccgg tacgggcact actaactatc aagactgcga aggcgatgat ggcacgctgg
     2461 ctcccggcgg cagagtaaaa accaaatgtt caataaagcc aatctatcgc tgtaaagtcg
     2521 tttttactca ctttatggca agcactcctc cggcaacgcg taccgtattg cgcttacgtc
     2581 cctacttcgg cgccatgcaa tacaccatga agctgtatga ttcgaccggc aagcgggttg
     2641 atattggatt agataagacc attatcgatg taacagcgag gattggcggc tcttaccggc
     2701 gcattgagca agaagttacc ttactgccca aaccgctcga ggggattgaa gctgtctttg
     2761 gcgacgataa ggtctgtaaa aagttcgaga ttatcaatac cggttccggc gaatttctcc
     2821 gagaggatag tattccgccc gactgcttgc tggattaagt ctcagcttag ctgcggcgat
     2881 gaaactgttc gtggaccttc cgcagttgcg ggtcggttaa atgagtatat atttgggtgg
     2941 ttgaaacgtt ggcgtggccc agaaggccct gtacgctgcg taaatcggca ccgttagaga
     3001 gcaggtcggt agcaaatgaa tggcgcagcg tatggggcgt tacgcgcttg gtaatgcccg
     3061 ccagcttggc atagcgactt acgaggcgct gtacgctgcg agcagtgagg cgagtataag
     3121 cgccgtcatc cagttcgtcc tggctcccgc cgtaatgaac gaacagcggt ttaaaattat
     3181 ccggtcggct ttttaaatag cggtcaatcc agagggctgc atccggtgaa ataaagatca
     3241 gccggtcctt ctgaccttta ccgcgaatac tgaactcccg ccgatccagg ttaatgtgct
     3301 cgcggtttaa gctaaccaac tcgcttaccc gcaagcctcc tgaaaagagt agttcaatga
     3361 tggctcggtc acgcaagccc accggtgtct caatagtgat aatttccagc agccgcttaa
     3421 cctcttcaac ctccaagaaa gtaacctggg gccgtttcac atttgccagc tcgatcttat
     3481 cgctggttaa ggcttcgata tcgcgcttag ctaaatattt aagaaagctg cgcaaggcaa
     3541 tgagatggta gttttgcgtc gatttgccaa tgggttcgcc actactgtcg gtcagtcgat
     3601 tcagccatac ccgccacttg cgcacgacat caggtgttag gttatgaatg gcaacgtcgt
     3661 cgctaaatgc aaccagccgt tgcaagtagc ggtcataatt ggcaatggtc ttctgtgacc
     3721 ggttttgttc aatctctaga tattcgaggt aatcggtcac caagtctgac agagaagtac
     3781 tgcttatg
//
LOCUS       DASVGB010000129         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_938954, whole genome shotgun sequence.
ACCESSION   DASVGB010000129 DASVGB010000000
VERSION     DASVGB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938954"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VF272_03975"
     CDS             <1..1104
                     /locus_tag="VF272_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7260058.1"
                     /translation="NHFANPSSPHSAGREARAALELARSAVSGTLGAKPVEIIVTSGS
                     TESAHIAISGAVGAFPNMSVAVAAIEHTAILQAATIFSEMPPAIIPCDPKGIVQPDTV
                     RKTITDTTVLVCVQYANNEIGTLQPVAAIAAEIAKIREQRIARGIDLPLYFYCDAAQG
                     GLLSLQVSRLGIDLLSMGGSKIYGPPGSGFLYIRTGTRLKPIFTGGGQEGGLRGGTEN
                     VAAAVGLAEALRLIQNSRTSESQRLKSLRDGLWRSIKNEIEGVVLNGSLDHRLPHNLH
                     LSFEGCEGEALVAHLDARGFAVATGSACTAANQDPSHVLLALGRTREQAESSVRISLG
                     RFTSVEEVEDFGPALNQAVADVRRHAMRNHRVQ"
     gene            1109..1702
                     /locus_tag="VF272_03980"
     CDS             1109..1702
                     /locus_tag="VF272_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7260059.1"
                     /translation="MKAIMAFLITTTVLLVLAALGVLGIGFFLSPQNQLRDADLIVAV
                     SGGETQQRTAEAVKLYQQGRASKLLFSGAAADPNAPSNAAVMRADALSQGVPDSAILI
                     EELSANTQQNAEMAVPIIRSLNAKTIILVTSPYHQRRASLNFHQAVGRDVTILNHSAS
                     DSSWRKSSWWQQEGTIGLTLSELQKTFYVWSVRRQES"
     gene            1767..2447
                     /locus_tag="VF272_03985"
     CDS             1767..2447
                     /locus_tag="VF272_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260060.1"
                     /translation="MVVVLFLGSQQVFAETLRSPNFEIDESFVGGGGQVEGGSPNFKT
                     DSAIGEVGVGNAASTNFQTDSGYITTNDPALTFIVNTTTINFGALSTSATASANSTFS
                     VINYTSYGYAVQTIGPPPQTGSYNLTGMSSMGPSQTGIEQYGINLRANTSPLTFGADA
                     TGGLGVAASGYTTPNNFKYVAGDTIASAPKSSGQTNFTISYIVNASSTTRGGAYSGVQ
                     SLVCTGTY"
     gene            2520..>2777
                     /locus_tag="VF272_03990"
     CDS             2520..>2777
                     /locus_tag="VF272_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260061.1"
                     /translation="MKSKLMNPTNRSLSAIIITGLLLSTFIPGLAQAAQLTNRKVTLG
                     TSQPSVSTTHNYTFTAPSATTVKSVDFTYCTTPSGTCTLPSG"
BASE COUNT          663 a          679 c          736 g          699 t
ORIGIN      
        1 aatcatttcg ccaatccttc atcgcctcat agcgctggac gtgaggcgcg tgcagctttg
       61 gaactagccc gttctgcggt tagtggtacg ctgggtgcaa aaccagttga gattattgtt
      121 acgagcggaa gcaccgagtc ggcacacatt gccattagcg gggcggtagg ggcgtttccg
      181 aatatgagcg tagccgtagc tgccatagaa cacacggcta ttttacaagc ggctacgata
      241 ttctctgaaa tgcctccggc aatcatcccc tgtgatccaa agggaatagt tcaacccgac
      301 acagtacgca aaaccattac tgacacgacg gtattggtat gtgtgcagta tgccaataat
      361 gaaatcggca cccttcagcc agtggctgcc attgccgctg agattgcaaa aattcgcgaa
      421 cagcgaattg cccgcgggat tgatttgccg ctttatttct actgcgatgc tgcccaggga
      481 ggcctgctca gcctgcaggt tagccgtttg ggcattgatt tgctaagcat gggggggtcg
      541 aaaatctacg gtcctcccgg cagcggtttc ttatacatac gtaccggtac gcggttgaaa
      601 ccaatcttta ctggtggcgg acaggaaggc ggtttacgcg gaggtactga gaacgtagcg
      661 gctgccgtgg gcttggctga ggctttacgt ttgattcaaa actcgcggac cagtgaaagt
      721 cagcgtttaa aatcgctgcg tgatggattg tggcgatcca taaaaaatga gattgaaggc
      781 gtggtgttaa acggttcgct tgaccaccgg ttaccgcata atctgcacct aagttttgaa
      841 gggtgcgaag gtgaagcctt ggttgctcat ctggatgctc gggggttcgc ggttgcgact
      901 ggttcggctt gtacggccgc caatcaagac ccctcgcatg tgcttcttgc actcggtcgt
      961 actcgcgagc aggcggaatc aagcgtgcga atcagtctgg gacgcttcac atcagtcgaa
     1021 gaagttgaag acttcgggcc tgccctgaat caggccgttg cggacgtgcg acggcatgcg
     1081 atgcgtaacc atcgggtaca atagcggtat gaaagcgata atggctttct taataactac
     1141 tacggtcctc ctcgtactgg cagcactggg tgttttgggt atcgggttct ttttatcgcc
     1201 tcaaaaccag ttgcgcgatg ccgacttgat tgtagcggtg agcggcgggg agacacagca
     1261 gagaaccgcc gaagccgtaa agctgtacca gcaggggagg gctagcaagc tattgttttc
     1321 gggagccgct gcagacccga atgcaccttc gaacgctgcg gttatgcgtg cggatgccct
     1381 cagtcaaggt gtgcccgaca gcgccattct tattgaggag ttatcggcta atactcagca
     1441 aaatgccgaa atggcggtgc caatcattcg aagccttaac gcaaaaacca ttattttggt
     1501 tacctctccg tatcatcagc ggcgggcaag cctcaatttt catcaggcgg ttgggcgaga
     1561 tgttactatt ttgaatcatt ctgcttccga tagctcatgg cgtaagagta gctggtggca
     1621 gcaagagggc actatcggcc tgactttgag tgagctgcaa aaaacctttt atgtttggtc
     1681 ggtgcgccgg caagaaagct agcgactaga ctttatccag ccatgggcgt cataatggaa
     1741 gcatgagaag tatacggtgg tgtgcgatgg tagtcgtact ctttttgggg agtcaacagg
     1801 tatttgcgga gacgcttagg tcgccaaatt ttgagattga tgaaagcttt gtaggtggag
     1861 gaggccaggt tgaaggcggt tcgccaaact ttaaaaccga tagtgcaatc ggtgaagttg
     1921 gtgttggtaa tgcggcctca actaactttc aaaccgattc cggctacatt acaacgaatg
     1981 acccggcctt aacctttatt gtcaacacta caactattaa ttttggggcg ctttcgacat
     2041 ccgccactgc tagcgccaat tcaacgttta gcgtcattaa ttacacgagc tatgggtatg
     2101 ctgtgcagac catcggcccg ccgcctcaga cagggagcta caacctgaca gggatgtctt
     2161 caatggggcc ctctcaaacc ggtattgagc aatacggcat taatttgcgc gcgaatacct
     2221 cgccgctgac atttggcgct gatgcaaccg gtggattggg ggtggcggcg agtggctata
     2281 ccactcccaa taactttaaa tacgtggctg gcgacacgat agcatcggcg ccaaaaagct
     2341 caggtcagac caactttacc atttcctata ttgtgaacgc atcaagcact acccgtggag
     2401 gagcctactc gggtgttcag tctttagttt gcactggaac ttattagcat aagcattttc
     2461 ttgactttct taagctgcta atgctacatt gtttctagta tgaaggtcta tacaagcaag
     2521 tgaaatcaaa actcatgaat ccaaccaatc gaagtctttc agcaatcatt attacaggat
     2581 tattgctatc aactttcatt ccaggcctgg ctcaggccgc tcagctaacc aaccgaaaag
     2641 ttacgcttgg tacttcacag cccagcgttt caaccaccca taattatacc tttacggccc
     2701 cttccgcaac gacggttaag tcggttgatt ttacctattg tacaactcct tccggcacct
     2761 gtaccctccc ctccggc
//
LOCUS       DASVGB010000130         6296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_375300, whole genome shotgun sequence.
ACCESSION   DASVGB010000130 DASVGB010000000
VERSION     DASVGB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375300"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VF272_03995"
     CDS             complement(<1..718)
                     /locus_tag="VF272_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7260062.1"
                     /translation="MLYLLVSCVLGLIVGSFLNVVILRVHAGKQFIRGRSACPHCHHQ
                     LSALEMIPVISWLVVRGRCRHCRKPISIQYPLVELVTAGLFALSYVQFKFNDVADVIF
                     FGLWLYVLGSMIVLAVYDIRWYLLPDKVLVPLIIPALLVAAGQAFMSQSYQPLLSSIG
                     AALLFGGFFYSLAAVSKGKWMGGGDIKLAFVMGLILGLQKTLLAMFIAFNTAALVGVL
                     LILMKRTKPGNVIPFGPFLIG"
     gene            complement(777..1208)
                     /locus_tag="VF272_04000"
     CDS             complement(777..1208)
                     /locus_tag="VF272_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7260063.1"
                     /translation="MKQLTDKKGFTLIEIVIVLAIAALILVAIFIAVQGAQASRRDAE
                     RRSAAGAIGALVEESAGNHKGSYPAQTGGATSTTFVTAVEGRTQKPNGGDFAPITVAS
                     TTLACPATLARDAVTVQTTANRTWLVAVGLESGDVFCTDNL"
     gene            complement(1293..2519)
                     /locus_tag="VF272_04005"
     CDS             complement(1293..2519)
                     /locus_tag="VF272_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7260064.1"
                     /translation="MIDFTYTAKDLTSGQVVKAEVKAESVSAAAKLLRSQNLFALDIT
                     PQGDGNILTRIGVSGRITTKEKVIFTRQLSTLVDAGLPIAKALRTVAQQINSVKFKRV
                     INSVVASVEGGSGLSNAFSQHPAVFNEIYISLVEAGETSGTLDKALTRLANQQEKDAA
                     IAAKIRGAMIYPAIVLALITAVVILMLTTVVPEVAKLYEDLKKELPLPTQILIFMSEV
                     LKNFWWAFLLAMGPAIYGIRKLLVTEGIQKEIDRYKLTMPLFGILFKKVYMARFARTM
                     HTLLASGIPMLEAMETARNAISNRLLAADVNEAINLVRGGKSLSEGLESSPHFLPLVA
                     QMSKIGEESGAIDNMLDKVATYYEDEVDEAVKNLSTTLEPVMMVVMGVIVMGVIVAVL
                     GPVYSLIGGGGVDQLK"
     gene            complement(2527..3597)
                     /locus_tag="VF272_04010"
     CDS             complement(2527..3597)
                     /locus_tag="VF272_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7260065.1"
                     /translation="MPAVAFKIEILLEEVIKTNASDLHVQVGLPPMLRVDGALRPVDE
                     APILDEAAIEKLLMSVLDEEQKQILTKDKEVDFSFSFGEYGRFRVNAFHERGNLAGAF
                     RLIPNQIRTLEELNMPKVVTSFASYPRGLVLVTGPTGSGKSTTLAAIIDKINREQARH
                     IITIEDPIEYAHHSKKSIVVQREIHYDTYSFGAALRSSLRQDPDVVLVGEMRDLETIS
                     AAITLAETGHLVLATLHTNSAAQSIDRMIDVFPPHQQQQIRIQVASMLQAICSQRLVP
                     TIGGGRVAAAEILVVTGAARNIIREGKTHQLDSVIQTGAEDGMQSMDRTLVSLVHEGK
                     ITFDEAKNFAVDLNELDRLMRA"
     gene            complement(3702..5474)
                     /locus_tag="VF272_04015"
     CDS             complement(3702..5474)
                     /locus_tag="VF272_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7260066.1"
                     /translation="MLSANTQQGVEELLLEKGIINKQQLDDFKLRALHESQPLMSILL
                     DAGLIDNEEMTRILAKASGLQYVNLSGVVVPQPILSMISRETAEAYMAVPFGKVDGRL
                     AVAMVDPSNLQAVDYLNRKVGEPLVTFMASQEGVQTVMLQYAAEITGDLKKIVQNATE
                     QDERNEKKTGAKSLQNLVQDAPISKALTTILEYAVNSRASDIHVEPREGDLRIRFRID
                     GVLQETMKLPKTIEPALVSRIKILSNLKIDEHRIPQDGQFVIKVSKQEVDLRIAISPV
                     VWGEQIVIRLLNKDSEMLTLESLGFKGRAYRNIIEGIKKPHGMTLSTGPTGSGKSTTL
                     YAALVAIKNVKINIVTLEDPVEYKMEGVNQIQINTDVGLTFASGLRSILRQDPNVIMV
                     GEIRDQETANLAVQSALTGHVVLSTIHTNSAAGVLPRLLDMGVEPFLVGSTVNTVIGQ
                     RLVRRVCPTCREEYPASDAEVISIKETLTGVLPKSTDDLEKAKTDYGYETLPLEGQSA
                     YTLYRGKGCKDCKEGYKGRTGIYEVFTMSEAMEDLLVKKATTSEIQIQAQKDGMVTMK
                     QDGYLKALNAHTTLEEVARVASDF"
     gene            complement(5476..5742)
                     /locus_tag="VF272_04020"
     CDS             complement(5476..5742)
                     /locus_tag="VF272_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260067.1"
                     /translation="MKKKEIIQLTVAGAIIAFAGLLFASQLGAPKSDSKKGRYTYEDV
                     RPIKSDYAPELIEKITDSEKVKDFYLPPDLNSGIGNQTPFKPIN"
     gene            complement(5739..>6296)
                     /gene="pilO"
                     /locus_tag="VF272_04025"
     CDS             complement(5739..>6296)
                     /gene="pilO"
                     /locus_tag="VF272_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7260068.1"
                     /translation="LMALLCIFAICITGYVAYILGKDAVMQTRLIAKKISVQKNYDKK
                     IASATSLIEKYHQLGPRQALIENALPITPDFPQIVSLIEFAAKSSGITLKSINPDASG
                     EQASDIGLSAATPVPQKVSFNAEIDGSYTNLFQFLQNLEASSRPMRVTAAEIKGNDAT
                     MKATLEVETAYQVKSDIKDPLKELE"
BASE COUNT         1575 a         1606 c         1438 g         1677 t
ORIGIN      
        1 ctcctattaa aaatggacca aacgggatga cattgccggg tttcgtgcgc ttcatgagga
       61 tcaacaagac tccaacaagc gctgctgtgt taaaggcgat gaacattgcc agcaacgttt
      121 tttgcaagcc gaggatcagc cccatgacaa acgccaattt aatatcccct ccccccatcc
      181 atttcccttt cgaaacggca gccaagctat aaaaaaagcc accaaacagc aaagcggcgc
      241 ctatggaact cagcaacggt tgataggatt ggctcataaa agcctgtcct gccgccacaa
      301 gcaacgctgg aataattaag ggtaccagca ctttatccgg aagaaggtac cagcgaatgt
      361 cgtaaaccgc gagcacgatc atgcttccca gcacatacag ccagaggccg aagaaaataa
      421 catcagcaac gtcattgaat ttgaactgca catagcttaa tgcgaacaaa ccagcggtaa
      481 ccagttccac gagcggatat tggattgata tcggcttacg gcaatggcgg caccggccgc
      541 gcactaccag ccagctgatg accggaatca tctctagggc agatagctga tggtggcagt
      601 gcggacatgc ggaccggccg cgaatgaatt gcttaccagc atgcacgcgc agaataacca
      661 cattaaggaa actcccgacg atcagtccga gcacacagga gactaaaaga taaagcatat
      721 gcttagtata acaaaaaccg ctcgtaatga gcggctttcg ctaggaacgg ttgctgttac
      781 aggttatctg tacagaacac gtctccactt tcaagtccaa ccgctaccaa ccatgtacgg
      841 tttgcggtgg tttgtaccgt gaccgcatct cttgcaagtg ttgcggggca tgccagagta
      901 gtgctcgcta ctgtaatcgg ggcaaagtcg ccgccgtttg gcttttgggt gcgtccctct
      961 accgcagtaa caaatgttgt tgatgttgca ccaccagttt gagctgggta actcccctta
     1021 tggtttccgg ctgattcttc aaccagcgca ccaatggcac cggcagctga gcggcgttca
     1081 gcatcgcgcc ggctagcctg ggcgccctga acggcaataa atatcgcgac caagataaga
     1141 gccgcaattg ccagaacaat cacgatctcg atcagcgtaa agcctttttt atctgttagt
     1201 tgtttcatgg tattattcac ctccttgtat tgttgcaaac atatagtgct acttggtcac
     1261 agtattatta aaaagcttac gtttgtcaag cattatttta gctggtcgac gccaccgccg
     1321 ccgattaagc tgtaaaccgg cccgagcacg gctacaataa cccccatcac aatcacgccc
     1381 attaccacca tcataaccgg ctctagggtt gttgaaaggt tttttacagc ttcatcaacc
     1441 tcatcttcat aataggtggc gactttatcg agcatattat caatcgcgcc ggactcttct
     1501 ccgatcttgc tcatttgggc taccagcggt aaaaaatgcg gactgctttc aagcccttca
     1561 gacagcgact tgccgccgcg taccaaattg atagcttcat ttacatctgc agccaacaga
     1621 cgattactaa tggcattgcg ggcggtttcc atagcctcaa gcattggaat gccgctggca
     1681 agcaaggtat gcatggtgcg ggcaaaacgc gccatataga ctttcttaaa taagattcca
     1741 aacaaaggca ttgtcagctt gtaacgatca atctcctttt ggattccctc ggttacaagc
     1801 agcttacgaa taccataaat tgccgggccc atcgcaagca aaaatgccca ccagaaattt
     1861 ttaagcacct cagacatgaa gataagaatt tgagtcggca gcggcagctc ttttttcaag
     1921 tcctcataca gttttgcaac ctcagggacc accgtagtta gcatcagtat aaccactgcc
     1981 gtaatcagtg ctaagacgat tgccggatat atcattgcac ctctaatttt ggcggcgatg
     2041 gcggcatcct tctcctgctg gtttgccagt ctcgtcaatg ccttatcgag tgttccggag
     2101 gtctctccgg cttcaactaa cgagatatag atttcattga atacggccgg atgttgggaa
     2161 aaagcatttg aaagccccga tccaccttca acactcgcga ccacggaatt aataacccgc
     2221 ttaaacttta cgctattgat ttgctgggca acggttcgca gggctttagc tatcggcaac
     2281 cccgcgtcca ccagcgtcga aagctgacgt gtaaagataa ccttttcttt agtggttatc
     2341 cgaccgctaa cacctatccg ggtcaagatg tttccatcgc cttggggcgt aatatctagt
     2401 gcaaacaaat tttgggagcg caacaacttg gcagccgccg aaacactttc agccttaact
     2461 tccgccttaa caacttggcc gctggttaaa tcttttgcgg tataggtaaa atcaatcatg
     2521 acgaccctat gccctcatga gcctgtccag ctcgttcaag tcgactgcaa agttcttggc
     2581 ttcatcaaag gtgattttac cctcatggac caaactcact aaggttctat ccatggattg
     2641 cataccatct tcggcgccag tttgaataac ggaatccagt tgatgggttt ttccttcacg
     2701 gatgatgttg cgggctgccc cggtaaccac caatatttcc gcggcggcaa cgcggccgcc
     2761 tccgattgtc ggcactaagc gttgcgaaca aatcgcttgg agcatgctcg ctacttgaat
     2821 tcgtatctgc tgctgctgat gaggaggaaa tacatcaatc atacgatcga tggattgagc
     2881 tgctgaattg gtatgcaggg ttgctaatac caggtggccc gtttcggcca aggtaatcgc
     2941 ggccgaaatt gtttccaggt cgcgcatttc ccctaccagc actacatcag gatcttggcg
     3001 aaggcttgaa cgcagagcag caccaaagga ataggtgtca tagtggattt cccgctgcac
     3061 gacaatagat tttttgctgt gatgggcata ttcaattggg tcttcaatgg taataatatg
     3121 gcgggcttgc tcgcggttta ttttatcgat aatcgcagct aacgtcgttg acttgcccga
     3181 tccggtaggc ccagtcacca gcacaaggcc cctggggtaa ctggcaaaac tggttacgac
     3241 cttcggcata ttcagttctt caagcgtacg gatttggttg ggtattaagc gaaacgcacc
     3301 tgcaagatta ccgcgttcat gaaaggcgtt tactcgaaaa cgcccgtatt caccgaagct
     3361 gaatgaaaag tcaacttcct tgtctttggt aagaatctgc ttctgctctt catcgagcac
     3421 cgacatcagc agcttttcaa tggctgcttc atctaaaatc ggagcttcat ctaccggccg
     3481 taaagcgcca tcgaccctca gcatcggcgg taagcctacc tgcacgtgaa ggtcggaagc
     3541 gttggtttta attacctctt caagtaatat ttcgatttta aaagcaactg cgggcataat
     3601 tgtagttcac cttttaagca taaccattta attctagagt accacatggc ctgtgagctg
     3661 tcacctttaa aagagctgtc ggatggccgc agccgcgacg cttaaaaatc ggaggccaca
     3721 cgggctactt cctcgagagt ggtatgggcg tttaaggctt tcaaataccc gtcttgcttc
     3781 atcgtaacca ttccatcttt ttgggcctga atttgaattt ccgaagtggt ggctttttta
     3841 actaagagat cttccattgc ctcgctcatt gtaaagactt cgtaaatacc cgtccggcct
     3901 ttatacccct ccttgcagtc tttacagcct ttacctcggt ataacgtgta ggcggattgt
     3961 ccttccagcg gcagggtttc atatccgtaa tcggtttttg ccttttctaa atcatccgtc
     4021 gacttaggca gcactcccgt caacgtctct tttatagata tgacttcagc atcactggcg
     4081 gggtattcct cacgacatgt cgggcacacc cttcgcacca accgctggcc gataaccgtg
     4141 ttaacggtag accccaccag aaaaggttct acacccatat caagcaagcg cggtagtaca
     4201 ccggccgctg agttggtatg gatggttgat agtaccacgt ggccggttaa ggctgattga
     4261 accgctaaat tggcggtttc ttggtctcta atttctccga ccataattac gttaggatct
     4321 tggcgcaaaa tcgatcgtaa accgctggca aaggttaggc caacatcggt attaatttga
     4381 atttgattca cgccttccat cttatattcc accgggtctt ccagggtaac gatattgatt
     4441 ttaacgtttt taatcgctac caaggctgca tacaatgttg tcgacttccc tgacccggtt
     4501 gggccggtcg aaagcgtcat gccatgcggc tttttaatcc cctcaataat gtttcgatac
     4561 gccctgcctt taaatccaag cgactccagc gtcagcattt cagaatcttt attcagcaaa
     4621 cgaataacaa tttgctcgcc ccaaacgacc ggagaaatcg caatacgcaa gtctacctct
     4681 tgcttgctga cttttatgac gaactgaccg tcttgaggta tgcgatgctc atcaatcttc
     4741 aggttagata gaatttttat gcgtgaaact aaggccggtt cgatcgtttt gggcagtttc
     4801 attgtttcct gcagcacgcc atcgatccgg aaacggatgc gtaaatctcc ctcccgcggt
     4861 tcaacatgga tatctgaagc ccgactatta acggcgtact ccaaaatggt ggtaagggct
     4921 tttgaaatcg gagcatcttg caccaagttt tgcagcgact tcgcaccggt ctttttttca
     4981 tttctctcat cctgctcggt ggcattttgg acaatttttt tcaaatcacc ggttatttct
     5041 gcggcatact gcagcataac cgtctgaaca ccttcctgcg aagccataaa cgtaaccagc
     5101 ggctcgccga ctttccgatt gaggtaatcg acggcttgaa gattagaggg gtccaccata
     5161 gccactgcca ggcgcccatc gactttaccg aacggaaccg ccatgtatgc ttcggccgtc
     5221 tccctggaga tcatgctcag tattggttgg ggcaccacaa ccccggaaag attaacatac
     5281 tgcaagccgc tagccttggc taaaatacgt gtcatttcct cgttgtcgat taatccggca
     5341 tcaagcaaaa ttgacatgag cggctggctc tcgtgtaacg cccgcagttt aaaatcatca
     5401 agctgctgct tgttgatgat acctttttca agcaataact cttcgacacc ttgctgggtg
     5461 ttggccgaca gcatcctagt ttatcggttt aaacggtgtt tggttcccaa tgccagagtt
     5521 taggtcaggc ggaaggtaga aatctttcac cttttcggaa tcggtaattt tttcaatcaa
     5581 ttcaggagca tagtctgact ttatcgggcg tacatcctcg taggtatagc ggcctttttt
     5641 cgagtcgctc ttgggggcac cgagctggct tgcaaagaga agcccggcaa acgcgattat
     5701 tgcgccggca acggtgagct gaattatttc tttctttttc actcgagctc cttcagcgga
     5761 tcttttatgt ccgatttaac ttgataagcg gtctctactt caagcgtagc cttcatagtg
     5821 gcatcatttc ctttgatttc ggcagccgta acacgcatgg ggcgggatga tgcttcaagg
     5881 ttttgtaaaa actggaatag gtttgtatac gaaccatcaa tttccgcgtt gaaactaacc
     5941 ttttgcggca cgggggtagc agccgataaa ccgatatcac tggcttgttc tcccgacgca
     6001 tcggggttga tggactttaa ggttatacct gatgacttgg cggcaaattc aattaagctc
     6061 acaatctgag ggaaatcggg cgtaatagga agggcgtttt caattaacgc ctggcgcggt
     6121 ccaagctggt gatacttttc aatcagactg gtagcactgg caatcttttt atcgtagttt
     6181 ttctgaacgc tgattttttt agcgatcaac ctggtttgca taaccgcgtc tttgcctaaa
     6241 atataggcga catacccggt aatgcaaatc gcaaatatgc acagaagcgc catgag
//
LOCUS       DASVGB010000131         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_377216, whole genome shotgun sequence.
ACCESSION   DASVGB010000131 DASVGB010000000
VERSION     DASVGB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377216"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VF272_04030"
     CDS             <1..725
                     /locus_tag="VF272_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260069.1"
                     /translation="GRIQIANTSNTGSPSAGSTVNFNTITNPSISGNNQNTFYIRVVT
                     YSDDAWTTPIDSGAVAGSVTNQIQVTASVDETLNFCAFQTGTTCANGTGTTVALGTLS
                     SSTATSSTSKLVAGTNATSGYVIQYTGATLTSGGNTITATGTAGATSSTGSSQFGINL
                     VANTAPTVGSAPSGGTGAASTNYATSNTFSFVPSTLTQVAAAAAASADTLFTVSYLAN
                     IPANQTAGAYSTTLTYICTATF"
     gene            728..1795
                     /locus_tag="VF272_04035"
     CDS             728..1795
                     /locus_tag="VF272_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260070.1"
                     /translation="MNVLSPNWTRRLAHIAIAALLVYLAVPFNVNAAQINARKLTLGT
                     SSPLASTSYTFNFTVPTTGTIVLSWEAQICTTPSGACTMPGGFVNSSSITQPTGLGDA
                     TGWTGNTATAGSLRMSNATNATTPAGSQTVTFNAVTNPSTPNQTLYARMTTYSAANWT
                     GSLDTGTVAASTANQIQLSGTMDESLVFCTGTSITGTNCATATGTTVNFGTFSTSATR
                     TGTSVMAASTNGQSGYSIALTGTTLTSGSNTITAMGTQSPNGAATASSIGTSQFGLNL
                     RANTVPTVGADLSGTGGGSYGTNYGIANSFRFFTGDSVAASAAATDANTFTVSYIVNV
                     AGGQPAGIYTANMTYICTATF"
     gene            1815..2783
                     /locus_tag="VF272_04040"
     CDS             1815..2783
                     /locus_tag="VF272_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260071.1"
                     /translation="MLRLLLGVWLSVVFIAVATPARAAAPPVNVPSAAQGLEISPPVL
                     ELNADPGQTLTVQIRLRDVSSGPLVVRATADDFGAKGENGDPQILLDEKEATRYSLKF
                     WVQQPSSFTLVPQEVKTIPVRIVVPANAEPGGHYGVIRFTGTPPELDGTGVSLTASIG
                     ALVLLRVSGDITEKLEVQEFFTSKTHEKASFFETGPIGFTERLRNEGSVHAKPVGYVA
                     VYNIFHKRVAQLAVSNPARNVLPDSIRKFEQQLETKRLFGRYTAQMNVRYGDNKKLSS
                     KTISFWVIPYKLILAVIIAIVLLVFLLRQGLRRYNQWVINQASQRR"
     gene            2826..>3710
                     /locus_tag="VF272_04045"
     CDS             2826..>3710
                     /locus_tag="VF272_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260072.1"
                     /translation="MGMRTGRLHPHHHTSYPVLALLLTLTGLTLLALSFRTSAATLST
                     SGSINLRGVMPGPPPSSAPTIDTPSNGQHFIKVPVALTGSCSSGLRVVVVRNNLLAGQ
                     AFCNTSSKYELLVDLIPGKNDLFARQYDTLDQPSPASPVISLFFDKPVGAITTSPGQP
                     PIPTLANPAVASQPFLISSTLSGLNLFPDTEFKLPIEIAGGIAPYAIHVDWGDGGSSL
                     LSASKFGSLNAPYTYATPGVYKVVIQATDANGAAAYFQTGLTVNGKTVVPPATTTPDT
                     KIVILWPIFIVIVALVLGF"
BASE COUNT          901 a          981 c          892 g          936 t
ORIGIN      
        1 acggtcggat tcaaattgct aatacctcaa ataccggctc gccaagtgct ggctccacgg
       61 ttaacttcaa tacgatcacg aacccaagta ttagcggcaa caaccaaaat accttttata
      121 ttcgggttgt aacctattcc gatgatgcct ggactacgcc aattgattcc ggggcagtcg
      181 ccggaagcgt aactaatcaa attcaagtta cggcttccgt tgatgaaaca ttaaacttct
      241 gcgcatttca aaccggcaca acctgtgcaa acgggaccgg tacgaccgtt gcgctcggga
      301 cactaagcag ctcgacggca acgagcagta cttcaaagct ggttgctggc accaatgcca
      361 catcagggta cgttattcaa tatacggggg caaccttaac cagcgggggc aataccataa
      421 cggcaactgg cactgctgga gccacctctt cgaccggcag ttctcaattt ggtattaact
      481 tagttgcaaa tacggcgccg acggttggtt cagcaccttc cggcggaacc ggtgcggcat
      541 ctaccaacta cgcaacctca aataccttct cctttgtacc gagcaccttg acgcaggtgg
      601 cggctgcggc tgcagcctct gccgatacgc tctttacggt ttcctatctt gcaaacattc
      661 ccgctaacca gacggccggc gcttactcta cgaccctcac ctatatttgt actgcgacct
      721 tttaaacatg aatgtcctat ctccaaattg gaccaggcgg ttggctcaca ttgccattgc
      781 cgcccttctt gtttatttgg cggttccgtt caatgtgaac gctgctcaaa ttaatgcccg
      841 caagctgaca ttaggaactt cgagcccctt ggccagcaca agctatacct ttaattttac
      901 ggtcccaacc acgggaacga ttgtgctaag ctgggaggcc caaatctgta ctaccccatc
      961 gggtgcgtgc actatgccag gaggtttcgt aaattccagt tcgattacgc agcctaccgg
     1021 actcggtgat gctaccggct ggacaggcaa tacagccacc gctggaagtt tgcgtatgtc
     1081 gaatgccacc aacgcaacga cgcctgctgg cagccaaacc gttactttca acgccgttac
     1141 taatccttct acgcctaatc aaacccttta tgcccggatg accacctatt cggcggccaa
     1201 ctggaccggt tcgcttgata cgggtacggt cgcagctagt acggccaacc aaattcagtt
     1261 aagcggtacg atggatgaat cgttagtatt ttgtaccggc accagtatta ccggcaccaa
     1321 ctgcgcaacg gcaactggca caacggtgaa cttcggtacc ttttcaacca gtgctacccg
     1381 aaccggcaca tccgtaatgg cggcatccac taatggccag agcggttaca gcattgcgct
     1441 taccggaacg acgttgactt caggatcaaa tactattaca gccatgggca ctcagtcgcc
     1501 aaacggagca gccactgctt cgagtattgg tacgtcgcag tttggattaa atttacgtgc
     1561 caataccgta ccgacggtgg gagctgatct aagtggcacg ggtggtggtt cctacggcac
     1621 taattacggc atcgctaact cgttccgctt ctttaccggg gattcggtag cggcatcagc
     1681 tgccgctacc gatgccaaca catttaccgt ttcttacatc gtaaatgttg ctggcggtca
     1741 gcctgctggt atctataccg ctaatatgac ctatatttgt acggcaacct tctgatacta
     1801 tgaagggtat gaaaatgctt cgcttactat taggtgtgtg gctttccgtg gtgtttattg
     1861 cagtcgctac ccctgcacgt gcggccgcac cacccgtaaa tgttccatcg gcagcacaag
     1921 gactagagat ttctcctccg gtactagaac tcaacgctga tcccggacaa acgctgacag
     1981 tccaaatccg cttgcgcgac gtatcaagcg gtcccttggt ggtgcgggca actgccgatg
     2041 attttggagc taaaggtgaa aacggggacc cccaaattct cttagatgag aaagaggcta
     2101 cccgttattc tttgaaattc tgggttcaac agccctccag ttttacctta gtaccgcagg
     2161 aagttaaaac gataccagtg agaatcgtag ttccggccaa tgccgagccg ggagggcact
     2221 atggcgtgat tcggtttact ggaacaccgc ccgaacttga tggtaccggt gtatcattga
     2281 cagccagtat tggggccttg gttttactac gggtttccgg tgatataaca gaaaaacttg
     2341 aagttcagga atttttcacg agcaagacac atgaaaaagc cagttttttt gaaaccggcc
     2401 ccattggttt tactgagcgc ttgcgcaacg agggaagcgt gcatgcaaaa ccggttggat
     2461 acgtagctgt ctacaatata tttcataagc gagttgccca gttggcggtc agcaatccag
     2521 cgcgcaatgt gcttcccgat agcattcgga agtttgagca gcagcttgaa accaagcggc
     2581 tatttggacg gtataccgcc caaatgaatg ttcgctatgg agacaataag aagctttcta
     2641 gtaaaaccat tagtttttgg gtaatacctt ataagctaat tctggctgtg attattgcca
     2701 tcgtcctgct tgtcttcctg ctacgtcaag gactgcgccg ttacaaccaa tgggtgatta
     2761 atcaagcatc acagcgtcga taaacccgga cgcctcggta tagcgcttat gctataatcc
     2821 cagcaatggg aatgagaacg gggcgcttac atccgcatca tcatacttcg tatccggttt
     2881 tagcgttact cctgacgctt accggattga ccttgctcgc tctgagtttc agaacatcgg
     2941 ccgccacctt aagtacttcg ggaagcatta atcttagggg cgtcatgcca gggccgccac
     3001 cgtcttcggc gcccactata gatacaccct caaacgggca acactttatt aaggtcccag
     3061 ttgccctaac cggcagctgc tcgagcggat tgagggtggt ggtggtgcgc aacaatttgc
     3121 tggctggcca ggcattctgc aatacatcaa gcaaatatga acttttagtc gatttgatcc
     3181 ccggtaaaaa tgatttattt gcccggcagt atgatacgtt ggatcagcca agccccgctt
     3241 cgccggtgat aagcttgttt tttgataagc cggtcggggc catcacaacc tcgcccggtc
     3301 aaccgccaat tcctacgtta gccaatccag cggttgcgag ccaaccgttc ctaatttcaa
     3361 gtactttgag cggccttaat ctttttcccg ataccgaatt taaactgccg atcgaaatag
     3421 caggagggat agcaccgtat gctattcatg ttgactgggg agatggcggc agtagcttat
     3481 tgagcgctag taagtttggg tcattgaatg cgccttatac ctatgctact ccaggtgttt
     3541 ataaggtggt aattcaggca accgatgcga acggggcagc cgcctatttt caaactggcc
     3601 taacggttaa tgggaaaaca gtggtcccgc ctgcaactac cacgcctgat actaagattg
     3661 ttatcctatg gccaattttt atcgttattg tagcgctcgt tctgggattc
//
LOCUS       DASVGB010000132         7924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_947124, whole genome shotgun sequence.
ACCESSION   DASVGB010000132 DASVGB010000000
VERSION     DASVGB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947124"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VF272_04050"
     CDS             complement(<1..529)
                     /locus_tag="VF272_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX7260073.1"
                     /translation="MQVESTTATPQKPLTSQLPPLVRELLHVRGLDDDASIQAFLFPN
                     YDTALADPYRMTDMEAAVVRITEAIARREQVAVYGDYDIDGIVSTALMAEVLQLHGLT
                     PLTYIPDRYDEGYGIHIKALEELKSQGVSLVISVDCGITSVAEAEWAQQNGLDLVITD
                     HHDVGAEIPQAAAVVN"
     gene            complement(593..877)
                     /locus_tag="VF272_04055"
     CDS             complement(593..877)
                     /locus_tag="VF272_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7260074.1"
                     /translation="MLEVFITSKTRRKIITVFTKYPDYKAHVRGLAQMIKEDPGNVQR
                     ELKKLEEIGFVKASRQSNTNVYTANQKFTLYRELQSIVLKSQANKRQAIS"
     gene            complement(972..1907)
                     /gene="secF"
                     /locus_tag="VF272_04060"
     CDS             complement(972..1907)
                     /gene="secF"
                     /locus_tag="VF272_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7260075.1"
                     /translation="MNLIGRRKLWYAVSLLIIIPGTISLVMFGLRLGIEFKGGQNLEV
                     KGQISQSDIQDKIGSLGLKDVSIVSSGANSVVRYRDEAQPKVQQSNHQALTKDLAAKG
                     HPEVSFETVGPSVSRDITRNALLSVALASVAIVLYIAFAFRNIPPPMTSWQFGLVAIV
                     AMLHDALLLIGLFSILGVMAGIEIDSLFITAVLTVIGFSVHDTIVVFDRIRENLRRER
                     GSFEEIVNTSILETLARSLNTSLTVLLALLALYLVGGESIKLFVLALIVGIASGTYSS
                     ILTASPLLVTWHNWQQKRALPSKLTKNQNLKSEPS"
     gene            complement(1904..3226)
                     /gene="secD"
                     /locus_tag="VF272_04065"
     CDS             complement(1904..3226)
                     /gene="secD"
                     /locus_tag="VF272_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7260076.1"
                     /translation="MMSLRVKFWLILLLAVVTAVLAYPKEGQLLQKIGLKNTNLSVKQ
                     GLDLQGGAELVFQAKDGELKKLTKTERETAMTSLIDVIQKRANPSGTAEVTVQRQGSD
                     RVIVDLPGVADVNEAINQIGRTANLMFLELAPGSNQPVQTNVTGKDVSRADPDFDPQT
                     GRPIVSLKMKGGQSTTKFGQLTTRINQQNGQLVTLLDDQIVFGPATVSQAITDGNAQL
                     SGNFKDIEAARSIAQQINAGALPIPIDLVQQRSVGPSLGKESVAKSLLAGVIGLIVVA
                     IFMVAYYRTAGFLAVLALIIYTLITLTVYKLTALTPYAIVLTLAGTAGFILSIGMAVD
                     ANILIFERMKEELRSGKSFVAATEAGFDRAWTSIRDSNAATLITCVILYLFGATIIKG
                     FAVTLGIGVLISLFTSVVISRTFLRLFIRTSLGRRPSAYGLKSSEVAA"
     gene            complement(3280..3729)
                     /locus_tag="VF272_04070"
     CDS             complement(3280..3729)
                     /locus_tag="VF272_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7260077.1"
                     /translation="MNRTASIIVGLILVVVIGVAVWLATKPAANETSQQSTSESQTTP
                     PAATPQPAADQPASESTVVTVNYDNNGFNPTTITIKSGQTLTFVNKTSSSIQPASDPH
                     PQHTENPELNVGNVSAGQSKSVTPKKTGTFGMHDHLNPSHTVAITVE"
     gene            3803..5758
                     /gene="priA"
                     /locus_tag="VF272_04075"
     CDS             3803..5758
                     /gene="priA"
                     /locus_tag="VF272_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7260078.1"
                     /translation="MSLPPKQYVLVAPLTYTGTASAGFTYEIDAAASIAAGQIVKVPL
                     GKRQSLGVITAVAPKQPSFPTKPVIEVLDMTPLPLHIVELASWLSAYYFASPKAVWQT
                     LLPSGITRKRRGAKVQPEGLILKPQNQPLTPDQQKAFRGIMKSDDTTSLIHGVTGSGK
                     TRLYLELTATHIKAGRSAIILVPEIALTPQLIALFEASFPGRVVPFHSGLTEAQKHQA
                     WQRALESQEPLVVVGPRSSLFLPLAKLGLIVMDECHESSYKQEQNPRYHTMPTAAKLA
                     RLAGAKLVLGSATPGLTEVYAAETGRITLYELPKRVENRPQPTSIIVDMRDSELRGPS
                     SLISRPLLDALRQTLDNGRQSLLFLNRRGTASSQICNHCGEVTLCPTCQLPLTFHADH
                     MKLMCHFCNYRQVPEAVCPHCGTSELRYAGGGTKRIETEIEQALPHVRVFRLDKDSAQ
                     PQELPKLYERLHQGEIDILIGTQMIAKGLDLPNIDTVGVVNADSMLHMPDFHSAERTF
                     QLLTQVSGRAGRGDRPGKVYIQTRTPDHPAIQAVKNGNFWDFAKNELAQRRLLGYPPF
                     RYLLKLTYSHKDEATAVSASQALYDTLKQNPAVRVLGPAPAFHARLGGQFHWQLIIKA
                     ADRRHLVVIASTLPSTWKTDLDPINVL"
     gene            5777..6331
                     /gene="def"
                     /locus_tag="VF272_04080"
     CDS             5777..6331
                     /gene="def"
                     /locus_tag="VF272_04080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7260079.1"
                     /translation="MAAKAPRDSIIHIPDPLLKRRSMRVGHIDKSTEALAREMILATL
                     DWEGTREHEFGAALAAVQIGKPYRVVVIRDDFENKDQKSFGVFINPEIVKQEGELEES
                     MEGCLSVPDIYGSVARYPKVKVRALNLNGQPIRITATGFLARVLQHEIDHCEGKLFTD
                     HVHDADKLFRLGSNGKFTKYSAGR"
     gene            6334..7260
                     /locus_tag="VF272_04085"
     CDS             6334..7260
                     /locus_tag="VF272_04085"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7260080.1"
                     /translation="MKSSLVFFGTGPVSLHCLDGIHDHFQIEAIITKPDRSSPSGMQH
                     PHPVRTWGEQQAIPVFQIGNEAELKALWDSHRFTSLTGLVVDFGITIPELVIDSFELG
                     IINSHFSLLPLLRGADPISFAILEGLPETGVSIMRIVPRMDEGDLLSQGSYRLPEGIT
                     TPDLTEKLGEISNQMLVRDLPRYIRGEITPRPQDPAITPTYSRKLAKQDGQIDWSKPA
                     AVLEREVRAFIGWPGSTAKLHGRDVTITATHIVVGKKSAIDNASPGTPIPHPEEPLLI
                     TTGKDSLAIDRLKPAGKREMSASEFVRGYSKP"
     gene            7284..7565
                     /locus_tag="VF272_04090"
     CDS             7284..7565
                     /locus_tag="VF272_04090"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX7260081.1"
                     /translation="MYKKLPKMVRQLIVLIIGVPIISLGVILLPLPGPGWLVILSGLY
                     IISREFAWARKYIDIIEAKLRQLAAKIKDKQSELKEREKPDSGKPKNSR"
     gene            complement(7571..>7924)
                     /locus_tag="VF272_04095"
     CDS             complement(7571..>7924)
                     /locus_tag="VF272_04095"
                     /inference="COORDINATES: protein motif:HMM:NF039821.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HEX7260082.1"
                     /translation="LRRCGMDLNDDFFNQIGLRGATDEQKDKLAEQLTQIVQNRIAVR
                     LVEVLTVDELEHFDELLESKGEDEAYEYLLKIYPEYPELLDGEVTQVKSALSQDVATV
                     GEVVEKMLTASKEEQ"
BASE COUNT         1993 a         2081 c         1938 g         1912 t
ORIGIN      
        1 ggttaactac ggctgcggct tgtgggattt ctgcccccac atcatggtga tcggtgatta
       61 ccaaatcgag tccgttttgc tgcgcccact cggcttctgc tactgaggta atgccgcaat
      121 caaccgaaat taccaagctt acaccttggc tttttagttc ctcaagcgcc ttaatgtgta
      181 taccatagcc ttcgtcgtaa cgatccggta tgtaggtaag cggggtcagg ccgtgcagct
      241 gcagaacctc agccatcaga gcggttgaaa caatgccgtc aatgtcataa tcaccataga
      301 ctgcaacctg ttcacgcctg gcaattgctt ccgtaatacg cacaaccgcc gcttccatgt
      361 cggtcatacg gtaagggtcg gctaaggcgg tatcataatt gggaaataaa aaggcttgga
      421 tggatgcgtc gtcatccaat ccgcgaacgt gcaacagttc tctcacgagc ggcggcaatt
      481 gggatgtgag aggtttctgc ggtgttgctg ttgtagattc cacctgcata ttttaagtat
      541 aacaggcttg gcaattttgt tgctacgcta aaagtctttc cctgtaaaaa atttagctaa
      601 tagcttgacg cttgttggcc tgcgatttca ggacaatgct ctgaagttct cggtataaag
      661 taaatttttg attggcggtg taaacattgg tgttcgattg gcggctggct tttacgaaac
      721 caatttcctc gagtttttta agttctcgtt gaacattgcc gggatcttct ttaatcattt
      781 gcgccaaacc acgaacatga gctttatagt caggatactt ggtaaacacg gtaataatct
      841 tgcggcgtgt ttttgacgta atgaaaactt ctagcattgt ttttcttaca accctttctt
      901 aggctctgct cactcggtac tactaatatg gtagcggacg aggatgataa atgcaaactg
      961 cttttgctta attagctcgg ttcggatttc aagttttggt tttttgtaag ctttgagggc
     1021 agtgcccgtt tttgctgcca attatgccat gtaacaagga gtggcgaagc ggtcaaaatt
     1081 gatgaataag ttcccgaagc aatgccaacg attaaggcca agacaaacag cttaattgat
     1141 tcacctccaa ccagatagag ggctaagagg gccaggagta ccgttagtga ggtattcaat
     1201 gagcgggcca aggtttccaa aatgctggtg ttcacaatct cttcgaaact cccccgctca
     1261 cggcgcaagt tttcccgaat tcggtcaaac accacaatcg tatcgtggac cgagaatccg
     1321 attacggtca aaacagcggt aataaacagc gaatcaatct caatgccagc cattactccc
     1381 aatatcgaga ataagccaat gagcagcaga gcgtcgtgga gcatggcaac gattgccacc
     1441 aggccaaact gccaggaggt cataggcggc gggatgttgc gaaatgcgaa ggcaatatag
     1501 agcacaatgg ctactgaagc cagagcaaca cttaggaggg cgttacgggt tatgtcacgg
     1561 ctaaccgatg gccctacggt ctcaaatgaa acttccggat gaccttttgc tgcaagatcc
     1621 ttggtcaagg cttgatgatt gctctgctga accttgggct gtgcttcgtc gcggtaccgg
     1681 actaccgagt ttgccccgct gcttacaata ctgacgtcct ttagacctaa acttccgatt
     1741 ttatcctgaa tgtcgctttg gctaatctgg cctttcactt caagattttg acctcccttg
     1801 aactcaatac ccaatcgcaa gccgaacatc accagtgaaa ttgtgccagg aataattatt
     1861 agcaaggaaa cggcatacca aagcttacgc cttccaatta aattcatgcc gctacctcgc
     1921 tcgattttag cccgtaagcc gacgggcgcc ggcctagcga agtgcggata aacaagcgca
     1981 ggaatgtccg gctaataact accgaggtaa acaagctaat taaaacgcca atccctagcg
     2041 taacggcgaa gcctttaata atcgtggcac caaaaaggta aagaataacg caggtaatca
     2101 gggtggctgc atttgaatcg cgaatcgacg tccaggcccg gtcgaaacca gcctcggtgg
     2161 cggccacgaa tgacttaccg gaccgtagct cttctttcat acgttcaaaa atcagaatat
     2221 tagcgtccac cgccatacca atacttaata tgaaaccagc ggtcccggcc agggttagta
     2281 caatcgcata cggagttaac gccgtcagtt tatagacggt aagagtaatt aaggtataaa
     2341 taatcaacgc cagcaccgcc aagaaaccgg cggtccgata atatgcaacc ataaagatgg
     2401 ctaccactat caggccgata acgcctgcga gcagactttt agcaaccgat tccttgccta
     2461 agctcggccc tacactgcgc tgctgcacca agtcaatcgg tatcggcaag gcaccggcat
     2521 taatctgctg ggcaatgcta cgagctgcct cgatatcttt aaaattaccg gaaagctggg
     2581 cgttgccatc ggtaatcgcc tgcgaaacgg ttgccggacc aaacacaatc tgatcgtcaa
     2641 gtaatgtcac cagctgtccg ttctgctggt taatacgcgt ggtaagctgg ccgaacttag
     2701 tggtggactg cccgcccttc attttcaaag acacgatggg ccggccggtt tgcggatcga
     2761 agtccggatc ggcccgggaa acatcttttc cggttacatt tgtctgcacc ggctgattac
     2821 taccgggagc aagctccaaa aacatcagat tagcggtgcg gccgatttga ttgattgcct
     2881 cgttcacatc ggccacgccg ggaaggtcaa cgattacccg gtcggagcct tggcgctgca
     2941 ccgtaacttc agccgtaccg gatgggttag cccgcttttg aataacatca atcaaagaag
     3001 tcatcgccgt ttcacgctcg gttttggtaa gctttttcag ctcgccatct ttagcctgga
     3061 agaccagttc ggcccctccc tgcaaatcaa gtccctgctt aacgctcaga ttggtgtttt
     3121 ttaaccctat cttctgcagc aactgccctt ctttcggata ggcaaggacg gccgtaacca
     3181 cagcaagcaa gaggattaac caaaatttaa cgcgtaagct catcatcgat tcgtttcagt
     3241 gtagcgcaaa acaaattggc cgacaatccg gccggacctt tattcaacgg taatcgcgac
     3301 agtatggctt ggattgagat gatcatgcat gccaaatgtg ccggttttct tcggtgttac
     3361 tgatttcgat tggccggccg atacattacc cacattcagt tcgggattct cggtatgttg
     3421 cgggtgcgga tctgaagcag gttgaatcga tgaagaggtc ttatttacaa atgttagggt
     3481 ttgcccggat ttaattgtaa tggtagtagg gttaaagccg ttattatcgt aattaacggt
     3541 aaccacggtg ctttctgaag ctggctgatc agccgcgggt tgcggtgtag cggccggtgg
     3601 ggtggtttgg gattcagagg ttgattgttg gctggtttcg ttggctgcag gcttagtggc
     3661 caaccataca gctacgccaa ttacgacaac gagaataagt cctacaataa ttgaggcagt
     3721 tcggttcata ctatttctcc ctttcgttta agacttacca ttataccaaa aatgcctccg
     3781 ccagataagc tacaatactc ccatgagcct gcctcctaaa cagtacgtgc tggtagcacc
     3841 gttaacctat accggcaccg cctcagccgg atttacctac gagattgatg cagccgcaag
     3901 catcgctgcc ggacagatag taaaagtacc tctcgggaag cgtcaaagcc tcggagttat
     3961 cacggccgtg gccccaaagc agccttcatt tcctacgaaa ccagtgatcg aggtactcga
     4021 tatgacaccc cttcctctgc acatagtgga actggcgtct tggcttagcg cctactactt
     4081 tgctagtcct aaggcggtct ggcaaacact cttgccgtcc ggcataacac gcaagcggcg
     4141 cggcgctaag gttcagccgg aaggacttat cttgaaaccc caaaatcaac cgctaactcc
     4201 agaccagcag aaagcctttc ggggcattat gaaaagtgat gataccacat cgcttattca
     4261 tggtgttacc ggatcgggca aaacccggct ctaccttgaa ctaaccgcca ctcacatcaa
     4321 agccggtcga tcggcaatta tcttggttcc ggaaattgcg ctaacgccgc agctaatcgc
     4381 tctcttcgaa gcgagctttc ccggaagggt tgtccccttt cattccgggt taaccgaagc
     4441 tcaaaagcat caagcgtggc agcgcgccct ggaaagccag gagcctttgg ttgttgtggg
     4501 accgcgttca agcctattcc tgccgctggc taaactaggc ttgatcgtta tggatgagtg
     4561 ccatgaatcc agctacaagc aggagcagaa cccccgctac cacacaatgc caaccgcggc
     4621 taagctcgcc cgcctagccg gagcaaagct ggtcctcggc agcgctacgc ccggcctaac
     4681 cgaggtctac gccgctgaga ccggacgcat taccctgtat gagcttccca agcgggttga
     4741 aaaccgaccg cagccaacat cgattattgt cgacatgcgc gactcggagc tgcgcggacc
     4801 aagcagcctt atctcgcgtc ccctcttaga tgccctaagg caaacattgg acaatggacg
     4861 ccagtccctg ctctttttaa atcgccgcgg aacggcttcc agtcagattt gcaaccattg
     4921 cggcgaagtc accctctgcc caacctgcca gctgccttta acctttcacg ctgatcatat
     4981 gaagctaatg tgccactttt gcaactaccg ccaggtgccg gaggcggttt gcccgcactg
     5041 cggaacgagc gaactgcgct acgctggcgg cggtacgaaa cgaattgaaa ctgaaattga
     5101 gcaagctctc cctcatgtgc gcgttttccg cctcgacaaa gacagcgccc agccgcagga
     5161 acttcccaaa ctctatgaac ggctgcatca gggcgaaatc gatatcctga ttggcaccca
     5221 aatgattgcg aaaggattgg atcttcctaa tattgatacg gttggcgtcg ttaatgccga
     5281 ttccatgctg catatgccgg actttcattc ggccgaacgc acctttcagc tgctgaccca
     5341 ggtttcaggc cgggccggac ggggcgatcg tccgggaaag gtctatattc aaacccgcac
     5401 tcccgaccac ccagcaatcc aagcggtgaa aaacggcaat ttttgggatt ttgccaaaaa
     5461 cgagctggct caacgccggc tcctgggcta cccgcccttt cgttatctgc ttaagctgac
     5521 ctattctcac aaagacgaag cgacagcggt tagcgccagc caggcgctct atgatacgct
     5581 aaaacagaac cccgctgtgc gggttttggg tccggcaccg gcgtttcatg cccggctcgg
     5641 cggccagttt cactggcaac ttattatcaa ggcagccgat cgccgtcatc tggtagtaat
     5701 cgcttccaca ttgccttcaa cctggaaaac agacctagac cccatcaacg ttttataaag
     5761 gaactacttc attcatatgg ccgccaaagc accccgagac agcattatcc atatccccga
     5821 tccgctttta aaacgcagga gtatgcgagt ggggcatatc gataaatcaa ccgaggcgct
     5881 ggctcgcgaa atgattctgg ctaccttgga ctgggagggc acccgcgagc atgaatttgg
     5941 cgcggcactg gcagcggttc aaattggcaa accttacagg gtggtggtaa tccgggatga
     6001 tttcgagaac aaggatcaga agagtttcgg ggtgtttatc aacccggaaa ttgttaaaca
     6061 agagggcgaa ctcgaggagt ccatggaagg ttgtttaagc gtaccagata tttacgggtc
     6121 ggtcgcccgc tatcctaaag taaaggttcg agcgctgaac ttgaacggac agccgattcg
     6181 cattaccgcc accggctttt tagcacgcgt gctgcagcat gaaatcgatc actgcgaggg
     6241 caagctgttt accgaccatg ttcacgatgc cgataagctg ttccggctcg gctccaacgg
     6301 taaattcacc aaatactctg cagggcgcta gcagtgaagt cttccctggt cttttttggc
     6361 accggtccgg taagcctgca ctgcttggac ggcattcatg atcatttcca gattgaagcc
     6421 atcatcacaa agcccgaccg cagctcgcca agcggtatgc agcaccctca tccggtacga
     6481 acctggggag aacagcaggc tataccggta ttccagattg gcaatgaagc tgaactaaaa
     6541 gcgttgtggg actcacatcg gtttacgagc ctgacgggtc tggtcgttga ttttggtatt
     6601 accattccgg aattggtaat cgatagcttt gagctgggca ttattaacag ccacttctcg
     6661 cttttgccgc tactgcgcgg tgccgaccct atcagcttcg ctatcctgga aggcttgcct
     6721 gaaaccggtg taagtattat gcggattgtt ccgcgcatgg atgaaggcga tctgctttcg
     6781 caggggtcgt atagacttcc ggaagggata actactccgg atttaaccga aaaattgggt
     6841 gagatcagca atcaaatgct ggtcagggac ttacctcgat acattagggg tgaaatcaca
     6901 ccgcggccgc aagatccggc tattacaccc acctacagcc gtaaattagc caagcaagac
     6961 ggccagattg actggtcgaa gccggctgcc gttctggaac gagaggttcg ggcatttatt
     7021 ggatggcccg gcagcactgc caaactgcat gggcgcgatg ttaccataac cgctacccac
     7081 atagtggtgg gtaagaagtc agcgattgat aatgcctccc ccgggacacc aattccccac
     7141 cccgaagaac cgctgctcat aacaaccggc aaggattcgc tagccattga ccggcttaaa
     7201 ccggcaggaa agcgtgaaat gagcgcaagc gagttcgttc gaggctattc caagccgtaa
     7261 tcacatatcg tacaatgcaa catatgtaca agaaactccc caaaatggtg cgccagctta
     7321 tcgtcctcat tataggcgta ccaattatta gcctcggtgt catacttttg ccgcttcccg
     7381 gaccaggatg gctggtgatt ctatcgggtt tatatatcat ttctcgagag tttgcctggg
     7441 cccgcaagta catcgatatt attgaagcga agctgcgcca actggccgca aaaattaaag
     7501 acaagcagag cgagctgaaa gagagggaaa aaccggattc aggaaagccg aaaaatagcc
     7561 gttgaggtaa ttactgctct tctttggatg cggtcaacat tttttcgaca acctcgccaa
     7621 ccgtagctac atcttggctc agcgctgact taacctgtgt aacctcgcca tcaagcaact
     7681 cagggtactc aggatagatt ttcaggaggt actcgtatgc ctcatcctcc ccttttgact
     7741 ccagaagctc atcgaaatgc tcaagttcat cgacagttaa aacctcgacc aaacgcacag
     7801 ctatccggtt ttgcacaatc tgggttagtt gctcagctag tttatccttc tgctcatcgg
     7861 tagcccctcg aaggcctatc tgattaaaaa aatcatcgtt taaatccatg ccacacctcc
     7921 ttag
//
LOCUS       DASVGB010000133        12761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1090448, whole genome shotgun sequence.
ACCESSION   DASVGB010000133 DASVGB010000000
VERSION     DASVGB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 12761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12761
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090448"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /gene="glmS"
                     /locus_tag="VF272_04100"
     CDS             complement(<1..1387)
                     /gene="glmS"
                     /locus_tag="VF272_04100"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7260083.1"
                     /translation="MCGIIGYVGLKQAAPILLSGLRRMEYRGYDSAGIALLNNSAIEV
                     IKRKGKIAELAAAVPDDAAGSTGIGHTRWATHGNPSERNAHPHVAGKVALVHNGIIEN
                     YAELKEKLAGFGVKSYHSDTDTEVLAHLINHELSEGRKLPEAVRRSLSQVEGTFGLAV
                     ISADDPGVMVAARRGSPLLLGIGDDSMYIASDASAIIGHTDRVVYLEDDQVAVCRARD
                     YRLFDLSRRTHQPSVTTLKLELAAIEKAGYDHFLLKEIMEQPTSVANALRGRLDLKGG
                     TSHLGGLNVHDNFLRQQERFLIIGCGTAYYAGLVGKYLLERMTGIPVDVEVGSEFRYR
                     DPALTPGTLAILISQSGETADTMACLRELKRRGVPTLGLVNVVGSSIARGVDGGVYLH
                     AGPEISVASTKAFTAQVVGQLMVGLQVGKARNLSLRTGREIVRALESLPDAVAEALKL
                     APQVEKLAKKYA"
     gene            complement(1391..2725)
                     /locus_tag="VF272_04105"
     CDS             complement(1391..2725)
                     /locus_tag="VF272_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX7260084.1"
                     /translation="MKFGAHVFRAYDIRGIVPDELDADAAEQVGRAFAGHLNPHTVVV
                     GRDVRLTGEELQGRAMEGLIRSGVDVVDIGQVSTDAFYYACATKSLPGIMVTASHNPA
                     EYNGFKLVKDMPFLVMAKEFKTYVLEESYNDAEKPGTMHTESVVDAFIDHVLTIVPAE
                     NLRPLKVVIDGSSGSQGPVWERLADKLPISIIPLFFEPDGTFPGHGNDVIQPENQAPL
                     RQKVLETGADLGLIFDPDGDRCLLVDDRGETVPGDFVTALLSAEILKHHPGGTVLYDI
                     RASDVVPAMIEEAGGKAFVWKPGHVYIKQKMKELGAVFGGEVSGHYYFRDFWGADCGL
                     LAGMTILEYASNLSGKLSQKVEELESIYHLSGEINSSVPNIPAAIARIRERYHDAKMN
                     ELSGLEIRYPDWRCVVRPSDNEPLIRLTLEAESQQKMEEKRDEVLALIRQDA"
     gene            complement(2737..4479)
                     /locus_tag="VF272_04110"
     CDS             complement(2737..4479)
                     /locus_tag="VF272_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX7260085.1"
                     /translation="MNMSVLLRYRWFSLALAIIGLGFLLLIGFSAAYAGKIYPGVSVN
                     GLHVGGLSREEALAKLDLESQKYNSQQLQVRYYETNLRIPVKALGVSYRPQAVERAVR
                     FGREGGVAQQLSNRLRALSGRTTSYTNYHYKDERLAPFLAHIDEDVTRPVANASLTFI
                     NGAVGIDPSHPGYRVDVGRLTASINRRLEVMSSDPVVAPVYTVTPTIDNAALQSARHQ
                     ADTYATSALALKTGNLTSIIDQSKILSWIDVTTPAGRTDLPIDPINQFYDAFVGPEVH
                     LTLSRSKVAAYVSEFAAKIDKTPQNAVLTMQNDKLTIAQPSQDGVSLDQVQSVGRVIA
                     ALGKPGTDRSVTLPGKVTPAEVNERNLDSLGIKERISQGQTFFPGSPSTRLTNVRVGQ
                     ALYNGILLKPDEVFSFGKILGDVGPAQGYVPELVILADRTEKQYGGGLCQVSSTAYRA
                     ALLAGLPILQRVNHSFAVSYYTAPYGVPGIDATIYYPQVDFKFKNDTGHYILMQTHMQ
                     GTTLTFDFFGTKTKTGEIRGPSFISGSGDPKQASHTVFYRDIKDLSGTVIKTDTVHTF
                     YKPSTDFPIEKQFN"
     gene            complement(4521..5570)
                     /locus_tag="VF272_04115"
     CDS             complement(4521..5570)
                     /locus_tag="VF272_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7260086.1"
                     /translation="MIIVIIAGGSGTRLWPLSQANYPKHLLALTGKTSLLQNTVARAR
                     KITPTVYVITERSQVGEVHNQLPDVPGNYIIAEPGRRGTASCIVLALAVLAENHPPTE
                     PVVFFHADSHIADQEEFAKAVEAAAGASAQHSRITLIGVRPTYAATGFGYIHRGAPVK
                     SRQELYEVKSFMEKPNAATAHKYIKEGTYLWNIGLFAAPIETWKKAFKQYAPDLAEGY
                     EALATSMDDPAALSERYLALKNQPIDTAVIEKAEGLLVVPGAFDWADIGSFQDLHKIL
                     QDKNHNVIQGDVSFKDSQNVMVYSTTGKPVIAIGLSDLVVVDTPDGLLVCDKSRSQLV
                     GDAAKKLQSRKAAAK"
     gene            5638..6609
                     /gene="galE"
                     /locus_tag="VF272_04120"
     CDS             5638..6609
                     /gene="galE"
                     /locus_tag="VF272_04120"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX7260087.1"
                     /translation="MKVFIAGGAGYIGGTTAHLMRDKGHEVIIFDNFSTGHKHNVDDF
                     ECIAGDITNRSALESALQGKNFDLVLDFAAKLRIAESVAHPHDYFTTNTMGTLNVVES
                     ALKSGVNHIIFSSTAAVYGEPEKVPIDEMAAIRPVNPYGMSKFLAENILRSYQATHAL
                     NWVAFRYFNAAGAYKQIGDYPDTQHLIPCILDALHDKRELKVFGSDYDTPDGTCLRDY
                     IHVVDVAEAHVLAAEAMVAGKKLNQAINLGTNHGSSVLEIIQAVEKAAETSVPYRLTA
                     RRPGDSGSLIASNLIANQVLGWQPQFGLERTVADAVSWHNDFFIKAS"
     gene            6681..7229
                     /locus_tag="VF272_04125"
     CDS             6681..7229
                     /locus_tag="VF272_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260088.1"
                     /translation="MKKTAKNKTIITALVIIALVALSGAAYVKVGQKPAEQTATNAET
                     SASTPSPGPAGPAHDTPTSASPAASTPQPNPSHPASTTLAKPIGPNNNTSSVSLSGAT
                     SMESVCRSVPGASCYIQASKSGATIRVSDAKIVGSSAGSDGVIFSWDAKQLSAGQWQI
                     QAIATKDGQTAASDSQLLRVQP"
     gene            7229..9220
                     /locus_tag="VF272_04130"
     CDS             7229..9220
                     /locus_tag="VF272_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260089.1"
                     /translation="MKLFRLSLFALLLICTSLGLSNTAHAVDYSNSNMMDDQIFDNTG
                     SMSEAQIQSFLASKGPCLPNFQTPNFYFDGTSWHYGDISSTTPPSDSVNYPHRWQAAF
                     GPATIGASTAIYKASRQWGLNPQVILATLQKESSLVAGTACDGWRYNSAMGYGCPDSG
                     GCNAKYVGFTRQVLWGSWQLKFNKERAYGNTAWDGDDAITYVGYMTEGDRRRCGTCPL
                     VHYDGNATIDGQQIHIENGTTASLFTYTPHLNQSFPGIFESWFGSVRGITYAWSYAGQ
                     TTNKSLTSIRAGETATFTVSARNTGFNTWTNSGPNPVRLGASRPNDRSSIFATNTWVS
                     PARPATLKEASVAPGGTGTFTFTVKMPATGGNYREYFNLLVEGKMWLNDPGLYYDITV
                     IPDVYSWSRVGQAVYANSSKAAPVDAGNLSPGQTVWFVLVAKNTGNTAWLNSGTNPVR
                     LGTEGPKDRHSRYVSPGWLGQSRPANLSEATVAVGANGTFEFPVTVPAGGGTFIERFN
                     LVAEGVDWMNDVGLSYFTRVNHGYSWSLISQYAYTNSAKTTPINLANLTPGQNAYVGI
                     VARNTGSSTWYRTGAYPIDLGTSRPLDRISAFTAAGWLGGARPARMVETAVAPGQNAT
                     FEFNFNASAAGTYREYFTPVAEGITWMNDIGLNFYSVVH"
     gene            9278..10480
                     /locus_tag="VF272_04135"
     CDS             9278..10480
                     /locus_tag="VF272_04135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7260090.1"
                     /translation="MYKPKEQLGVGHLAISDKEKSYVNQALDSNRLSYGPFSKRFEQE
                     FAAIHDSRHAIMSNSGTSSLQVALAALSEKYGWKAGDEVIVPAVTFIATSNVAIMLNL
                     KPVFVDVDPKTYNINPALIEAKITPRTKAILPVHLFGLPADMKPIMEIARKHKLRVIE
                     DSCETMFVKAYDKPVGSFGDIGCFSTYVAHFLVTGVGGLSITSDDDLAVMMRSLCNHG
                     RDGIYLTIDDDKDKHGKELSTIIERRFNFVRLGYSYRVTELEAALGVAQLEIKDSILE
                     ARQDNAKYLIDGLKPLEAAGHIQLPSVPNYAEHAFMLFPVVVKDPALGSSMLTYHLEE
                     HAIETRPMMPLINQPFYVERFGNIEGAYPTAQWINKGGFYIGCHQGFTHEDLDYIIQI
                     FNDFFRGK"
     gene            10480..11418
                     /locus_tag="VF272_04140"
     CDS             10480..11418
                     /locus_tag="VF272_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX7260091.1"
                     /translation="MSFWKDKQVVVTGGHGFLGGHLAKRLNAEGAKISTPPHSEYDLV
                     KLEDVRQMYDDHPGTEMVIHLAAKVGGIGANREHPGSFFYDNLMMGAQLMHEAYLRKV
                     PKFVALGTVCAYPKHTPVPFKEDDLWNGYPEETNAPYGLAKKMMLVQSQGYRQEYGFN
                     SIFLLPVNLYGPGDNFDPATSHVIPALIKKCVDAITSNTEEIVVWGDGSPTREFLYVE
                     DAVQAILLAAEKYNSSEPVNLGSSFEVSIKELVEIIAKETGFNGKIVWDASKPNGQPR
                     RKLDTSRAEQEFGFKSATTFEEGLRETISWYQRNSS"
     gene            11419..>12761
                     /locus_tag="VF272_04145"
     CDS             11419..>12761
                     /locus_tag="VF272_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX7260092.1"
                     /translation="MDALTKKTARNSSYSTIAFIWPILLAFFVTPFIVHALGTEQYGI
                     YSLVIAFVGFFGFLDLGIAPSLVKYVSEYNARKDDNAINLLFNSALFLYLTIGIMAAF
                     LIAGFGRFYAPDVWKTSAENAPLLQTVLYIAAIGFLINMVLTAFSALPSALQRFDITS
                     KINLVVTTISTGLTVLLLLAGEGLVAIVALGIFTSLAALLVYHFINRRLLPALRLKPH
                     IDRKMLKLVLVFGGYATIGTLASVILLQLDKFIIGATLGASAVAFYVIPGNLAVKIHG
                     TISALTAIIFPLSSELFATGNLERLHSLYFRATRLVLTFITVAVTPMYVFAHPFLLHW
                     IGVSFAQNSTLVMQLLLATYSILSLTAIPFFVLYGAGKPKMAALFSVISGVLNVALIL
                     ILIPRFGINGAAFAYLLSVLPCILFVRYVEKKVLRLSVRAFYVGIGPRLMVLFGAS"
BASE COUNT         3063 a         3446 c         3123 g         3129 t
ORIGIN      
        1 tggcatattt tttcgctagt ttttcaacct gcggtgcaag cttgagtgcc tcagctacgg
       61 catccggcaa ggattccaat gcccgcacaa tctctcgtcc cgtgcgcaaa cttaaattac
      121 gggcctttcc tacctgcaat ccaaccatta actggccgac tacctgagcc gtaaaggctt
      181 tggttgaagc caccgagatt tccgggccgg catgcaaata aacgccgccg tcgactccgc
      241 gggctatcga tgaaccaacc acattaacta aaccaagggt tggcaccccg cgccgcttaa
      301 gctcgcgcaa acaagccatg gtgtcggccg tctcccctga ctgtgatatc agtatcgcta
      361 aggtgcccgg cgttaaggcc ggatcgcggt aacgaaactc cgaaccgact tccacatcga
      421 ctggaatacc ggtcatccgc tccaataagt atttcccgac cagtccggcg taataagcgg
      481 tgccgcagcc aataatcaaa aaacgctctt gctggcgtaa aaagttgtcg tgtacgttca
      541 agccccccaa gtgactggtc ccgcctttca gatccagccg accgcgcagt gcattggcta
      601 ctgaggttgg ctgctccatg atttctttta ggaggaaatg atcataaccg gccttttcaa
      661 tagccgcaag ttcgagtttc aaagtagtta ccgagggttg atgggtgcga cggcttaaat
      721 caaacagccg atagtcgcgg gcacggcaaa ccgctacctg gtcatcttct aaataaacca
      781 ctcgatcagt atggccaata atagcgctgg catcgctggc aatgtacatc gaatcatccc
      841 ctattcctag aaggagcgga ctgccacggc gggctgccac catcacgccg ggatcgtcgg
      901 cgctaataac ggccagtccg aaggtccctt caacttggct taaactgcgg cgcaccgctt
      961 ctggcagctt gcgtccttcg ctgagctcat gattaatgag atgcgccagc acttccgtgt
     1021 cggtatcgct gtggtaggat ttaacgccaa atccggccaa cttttccttt agttcggcat
     1081 agttttcaat aatgccgtta tgtaccaacg ccaccttgcc tgctacgtga gggtgagcat
     1141 tgcgttcact ggggttgccg tgcgtcgccc agcgggtatg gccaatcccg gtcgaaccgg
     1201 cagcatcatc cggcaccgcc gccgccagtt cggctatctt gcctttacgc ttaataactt
     1261 caatagcact gttgttaagc agagcgatgc cggcggaatc gtaaccacgg tattccatgc
     1321 gccgcaggcc ggacagcaaa atcggggcgg cctgcttaag accgacgtag ccaataatgc
     1381 cacacatgcc ttacgcgtcc tgccggatta aggccagtac ttcgtcgcgc ttttcttcca
     1441 ttttctgctg tgattcggct tcaagcgtta ggcggatcaa cggttcatta tcggaaggcc
     1501 ggaccacgca gcgccaatca ggataacgga tttccaaacc gcttagctca ttcatttttg
     1561 catcgtgata gcgctcgcgt atccgggcaa tagccgcggg aatgttcggc acgcttgaat
     1621 taatttcacc acttaaatga tagattgatt ccagctcttc aaccttttgg cttaacttgc
     1681 ccgaaagatt gctagcatac tcaagaatgg tcatcccagc cagcaggcca cagtcggcac
     1741 cccagaaatc acggaaataa taatgcccgg agacctcgcc gccaaacacc gctcccagct
     1801 ccttcatttt ctgctttata tatacatggc ccggcttcca cacaaacgcc ttaccgccgg
     1861 cttcttcaat catagctggc accacatcac tggcgcgaat gtcatataac acggttccgc
     1921 cgggatggtg tttaagaatt tccgcagaaa gcagagccgt cacaaaatcg cccggaacgg
     1981 tttcaccccg gtcatcaacc agcaggcacc ggtcgccgtc agggtcaaaa atcaaaccaa
     2041 gatcagcacc ggtttcaagc actttttgac gcagaggcgc ctggttttca ggttgaatca
     2101 catcgttgcc gtggccggga aaggtgccgt ccggttcaaa aaatagggga ataattgaaa
     2161 tcggtagctt gtcggccaga cgctcccaca ccggtccttg ggagccgctg gacccatcaa
     2221 tcaccacctt cagcggacgg aggttttcgg ccggaacgat tgtcaatacg tgatcaatga
     2281 atgcatcaac caccgactcg gtatgcatgg tgcccggctt ctccgcatcg ttatagctct
     2341 cttctagcac ataggtttta aactctttgg ccatcactaa aaacggcata tcttttacca
     2401 atttgaagcc gttgtattcc gccggattat gcgaagccgt aaccataatc ccgggcaggc
     2461 ttttagtggc acaagcataa taaaaagcgt cggttgaaac ttggccaata tccaccacat
     2521 caacccccga gcgaatcaaa ccctccattg cccggccttg cagctcttcg ccggtcagac
     2581 gcacgtcgcg gccaactaca acggtgtgcg gattaagatg cccggcaaat gcccggccga
     2641 cttgctcggc ggcatcagca tccaattcat ccggtacaat accgcggatg tcataggccc
     2701 taaatacatg tgctccaaat ttcacagatt ggttccttag ttaaactgct tttcgatggg
     2761 aaagtcggtg ctgggtttat agaaagtatg gaccgtatcg gtcttaatga cagtgccgct
     2821 taagtctttg atgtcgcgat agaagaccgt atgcgaagcc tgcttcggat ccccgctgcc
     2881 gctgatgaag gaaggtccgc gaatttcccc ggttttggtt ttcgtgccga agaagtcgaa
     2941 ggttaaggtc gtgccctgca tatgggtctg cattaatatg tagtgtccgg tatcattctt
     3001 aaacttaaaa tctacttggg gatagtatat ggtagcatct atccccggga caccataagg
     3061 agcggtataa tagctgaccg caaatgaatg gttaacccgc tgtaagattg gcaatccggc
     3121 tagcaaggct gcccggtagg cagtggagct aacttgacag aggccgccgc catattgctt
     3181 ttcggtacga tcggctaaga taaccaactc cggtacgtaa ccttgcgccg gccccacatc
     3241 acccaaaatt ttaccgaaag aaaaaacctc gtccggcttt aataaaatac cattatagag
     3301 cgcttggcct acccgcacat tcgtaagccg ggttgaaggt gagcccggga aaaaggtttg
     3361 cccctggctg attcgctcct taatgccgag gctatcaaga ttgcgttcgt tgacttcggc
     3421 aggtgttacc ttgcccggca gggttaccga acgatcggtg ccgggcttgc ccaatgcagc
     3481 aatgaccctc cccaccgact gaacctggtc gagcgatacc ccatcctggc tgggctgggc
     3541 aatcgttagc ttgtcgtttt gcatcgtcaa taccgcattc tgcggcgttt tatcaatttt
     3601 ggccgcgaac tctgacacat aggcagctac cttggacctg ctgagcgtta gatgaacctc
     3661 cgggccgaca aatgcatcat aaaactggtt gataggatcg atgggcaaat cggtacggcc
     3721 tgccggagtg gtaacatcaa tccagctgag aatcttgctt tgatcaataa ttgaggtcaa
     3781 attgccggtt ttaagggcta gggccgatgt tgcataggta tctgcttgat ggcgcgcgga
     3841 ttggagggca gcattatcga tcgtgggtgt aaccgtataa accggcgcca ccaccggatc
     3901 cgagctcatg acttccagcc gtcggttaat cgaggcggtc aggcggccga cgtctacgcg
     3961 gtagcccggg tgagaagggt caatgccaac cgctccgtta ataaaggtta agctggcgtt
     4021 agccaccgga cgggtaacat cttcatcgat gtgcgctaga aatggcgcca aacgctcatc
     4081 cttataatga tagttcgtat agcttgtggt ccggccggaa agcgcccgca agcgattgct
     4141 taactgttgg gcaaccccgc cctcgcggcc aaaacgcacc gcacgttcga ccgcctgggg
     4201 cctataacta acgcctaaag ccttaacggg aatgcgtaaa ttagtttcgt agtagcgcac
     4261 ctgcaactgc tgcgagttgt acttctggga ctccaaatcg agcttagcta gagcctcctc
     4321 tctgcttaag ccgccaacat gcagcccatt cacacttacg ccgggataaa tcttgccggc
     4381 ataagcagct gaaaatccaa tcaatagcaa gaaccccaga ccaataatgg caagggcaag
     4441 ggaaaaccaa cgatagcgca aaagaaccga catattcata caacgtatag tatagctaaa
     4501 tcagccaaaa ccgcctgcat ttacttggcc gctgctttac gcgattgcaa ctttttagcg
     4561 gcatctccta ccaactggga acgcgatttg tcgcacacca ataaaccatc cggggtgtcc
     4621 accaccacca aatcggaaag gccaatagct ataaccggct tgccggttgt gctgtatacc
     4681 ataacgttct ggctatcctt gaagctaaca tcgccttgta tgacgttatg gttcttgtct
     4741 tggaggatct tatgcaagtc ctggaacgag ccaatatcgg cccagtcgaa cgccccgggc
     4801 actaccagta gtccttcagc tttttcaatg acagcggtgt caatcggctg attctttaag
     4861 gccaggtagc gttcgcttaa cgccgccgga tcatccatgg acgtagcgag cgcttcatac
     4921 ccttccgcca aatccggagc atattgctta aaggctttct tccaggtttc aatcggggcg
     4981 gcaaacaaac caatgttcca caagtaagtg ccttctttta tatatttgtg ggcggtagcg
     5041 gcattcggtt tttccataaa tgacttaact tcatacagct cctgccggct tttaaccggg
     5101 gcaccgcgat gaatgtagcc gaaaccggta gcagcatagg tggggcgtac gccaatcagg
     5161 gtgattcgcg aatgttgggc tgaagcaccg gctgccgcct ccactgcttt cgcaaactct
     5221 tcttgatcgg caatatgcga atccgcatgg aaaaaaacca ccggttcggt cggaggatga
     5281 ttttcagcca gcaccgccag tgccagcact atgcatgagg ccgtcccgcg acggccgggc
     5341 tcagcaataa tatagttgcc gggaacgtct ggtagctgat tatgtacttc acctacttgg
     5401 gatcgttcgg ttatgacata gacggtcggg gtaattttcc gcgcgcgcgc cacggtattt
     5461 tgcaataacg aagttttgcc ggttaaagcc agcagatgtt tagggtagtt agcttggctc
     5521 agcggccaca ggcgcgtacc tgatccaccg gcaataatca caattatcat ggttagatta
     5581 tacaggagtg atgagggtag ctagcaaatt cgtctgccaa ccagtaaaat gccacatatg
     5641 aaagtcttta ttgccggtgg agccggctac atcgggggaa ctaccgctca tctgatgcgc
     5701 gataaaggtc acgaggtcat catctttgat aatttctcca ctggccacaa gcacaatgtt
     5761 gacgattttg aatgtatcgc tggcgacata accaaccgca gcgcgctcga atccgccctg
     5821 caaggtaaaa atttcgacct ggttctggat tttgcggcca agctgcgcat tgccgaatcg
     5881 gtggcgcatc ctcatgacta tttcaccacc aatactatgg gaaccttgaa tgttgtcgag
     5941 tccgcactga agtctggtgt taaccatatt attttttctt caaccgctgc tgtatatggc
     6001 gagccggaaa aagttccgat tgatgagatg gcggcaattc gtccagtcaa cccctatgga
     6061 atgagcaagt ttttagctga aaacatcttg cgcagctacc aagcaaccca tgcattaaac
     6121 tgggtggcat ttcgttattt caacgccgcc ggagcctaca agcaaattgg cgattacccc
     6181 gacacgcagc acttaatccc ctgcatcctt gatgctcttc atgacaaacg cgagcttaaa
     6241 gtgtttggtt ccgactacga cactccagac ggcacgtgct tgcgcgacta tattcacgtg
     6301 gttgatgtcg ccgaggctca tgtgctggct gccgaggcaa tggttgccgg aaaaaagcta
     6361 aaccaggcga ttaacctcgg caccaatcac ggttcttcgg tcttggaaat tattcaggcc
     6421 gttgaaaaag ccgccgaaac cagcgttccc taccggctta cagcacgacg tcccggcgat
     6481 agcggcagtt tgattgcctc taaccttata gccaatcaag tgctcggctg gcagccgcag
     6541 tttggccttg aacgtacggt ggccgacgct gtgagttggc ataatgactt tttcatcaaa
     6601 gcctcatagc ctggctagtc tgatctgctc ttgattaaga taaggcttcc ttttatactg
     6661 cttaagcata ggggcgaaac atgaaaaaga ccgccaaaaa caaaacgatc attactgcac
     6721 tcgtaatcat agcgctcgta gctctgagcg gtgcggctta tgttaaggtg ggccaaaagc
     6781 cggctgagca gaccgccacc aatgctgaaa cgtccgcttc cacccccagt cccggtcccg
     6841 ctggtcctgc tcatgacact ccaacttcag catctcctgc tgcgtctacc ccccaaccta
     6901 atcccagtca cccagcttca actaccctcg cgaagcccat tgggccgaac aacaatacca
     6961 gtagtgtcag cctttccgga gcaaccagca tggaatcggt ctgccgttca gtgcctggtg
     7021 cttcgtgcta catccaggct agcaagagcg gcgcaacgat aagggtcagc gatgctaaaa
     7081 ttgtcggaag tagtgctggc agtgatggcg taatctttag ttgggacgct aaacagttat
     7141 ccgccggtca atggcaaatc caggcgattg ccaccaagga cggacaaacc gccgcttccg
     7201 attctcaact acttagggta cagccttgat gaaacttttc cgacttagcc tgtttgcttt
     7261 actgttaatc tgcaccagcc ttggactgag taataccgcc catgctgtcg actacagcaa
     7321 ctccaacatg atggacgatc aaatttttga taataccggc agtatgtcgg aagctcaaat
     7381 tcagtcattc ctggcttcca aaggtccatg cctgccgaac ttccaaacac ccaattttta
     7441 ctttgatggt acctcctggc actacggtga catttccagc actacccctc caagcgacag
     7501 cgttaactat ccccatcgtt ggcaggcggc atttggcccg gccactattg gcgcatctac
     7561 ggccatttat aaggccagtc ggcaatgggg gctcaatccg caggttattc ttgccacgct
     7621 tcaaaaggag tccagtttgg tagcaggcac ggcttgcgac ggatggcgct ataactctgc
     7681 tatgggctac ggttgtcccg attcgggcgg atgcaacgct aaatatgttg gttttacccg
     7741 ccaagtccta tggggatcat ggcagcttaa gttcaacaag gaacgcgcct atggcaacac
     7801 cgcatgggat ggtgatgatg ctatcactta tgtaggatac atgaccgaag gcgaccgaag
     7861 gcgttgcggc acctgcccac tagtgcatta cgacggtaat gccactatcg acggccagca
     7921 gattcatatt gaaaatggaa ctaccgcctc gctcttcacc tacactcctc atcttaatca
     7981 atcattcccg ggcatattcg agagttggtt cggctcagtt cgcgggatta cctatgcctg
     8041 gagctatgcc gggcaaacca caaataagtc gttaactagt attcgcgctg gggaaaccgc
     8101 tacctttacg gtgagcgccc gcaataccgg atttaatacc tggacgaata gtgggcctaa
     8161 ccccgtccgc ctgggagcta gccgcccaaa tgaccgctcc agcatctttg ccaccaatac
     8221 ctgggtgagc ccggccagac cagctacgct caaggaagct agcgtagccc ctggtggaac
     8281 cggtaccttc acgtttaccg tcaaaatgcc ggctactggc ggcaattacc gagagtactt
     8341 caatcttctc gttgaaggaa agatgtggct aaatgaccca ggcctctatt acgatattac
     8401 cgtaatacca gatgtttact cctggtcgcg cgtcggccaa gcggtttacg ccaactcctc
     8461 gaaagccgcc cccgtagatg ctggcaatct ttcgcctggt cagacggtat ggtttgtatt
     8521 agttgccaag aatactggca acaccgcttg gttaaatagt ggcactaacc ccgtccgcct
     8581 cggcaccgaa ggacccaagg atcgccatag ccgctatgtt agtccgggat ggcttggaca
     8641 gagccgccca gccaatcttt cagaggcaac tgtcgccgtc ggggccaatg gtactttcga
     8701 gtttcctgtg actgtaccag cggggggagg cactttcatt gaacgcttca accttgtggc
     8761 cgaaggagtc gactggatga acgatgtagg ccttagctat tttacgcgcg ttaatcacgg
     8821 ctacagctgg tcgctgataa gccagtatgc ctacaccaac agtgcgaaaa caacgcccat
     8881 caatcttgcc aatctcacac ctgggcaaaa tgcttatgtc ggtattgtgg cgcgtaatac
     8941 cggtagctcc acatggtacc gtaccggcgc ttaccctatt gatctcggca cctcccgccc
     9001 gcttgaccgc ataagcgcct ttacggcagc cggttggcta gggggagccc ggccagccag
     9061 aatggtggaa actgcggttg cgcccggtca aaatgctacg tttgagttta actttaacgc
     9121 ctcagccgcc ggcacctacc gtgagtactt taccccagtg gctgaaggca ttacatggat
     9181 gaatgacatt ggtttgaatt tctatagcgt agtgcattag acttccgaaa agaccctaaa
     9241 attgctatac tgaatcagtt aaatcccgga ggaattgttg tacaaaccca aggagcaact
     9301 tggcgttggc catttagcca tatccgataa agaaaaaagc tacgtcaatc aggccctcga
     9361 cagcaaccgc ttgtcttatg gccctttttc taagcgtttt gagcaagagt ttgctgccat
     9421 acatgactcc aggcacgcca taatgtctaa ctctgggacc agttccttgc aggttgcact
     9481 agctgcatta agcgaaaagt atggatggaa agctggagat gaggttatcg ttccagctgt
     9541 cacctttatc gcaacctcga atgtggctat catgcttaat cttaagccgg tgtttgttga
     9601 cgttgacccc aaaacttaca atattaatcc cgcgctgatt gaagcaaaaa ttaccccgcg
     9661 gacgaaggca attctgccgg tccacctttt tggtctgccg gccgatatga agccaataat
     9721 ggagattgcg cgcaagcaca aattgcgggt gattgaagat tcctgtgaaa ccatgtttgt
     9781 aaaggcctac gataaacctg tggggtcatt tggtgacatc gggtgctttt ctacttatgt
     9841 ggcacacttc ctcgtaacgg gcgttggggg cctgagcatc acgagcgatg acgatctagc
     9901 ggttatgatg cgcagcttat gcaatcacgg ccgcgacggc atttacctta ctatcgatga
     9961 cgataaagac aagcatggca aggaactatc aaccattatt gagcggcgct ttaactttgt
    10021 gcggcttgga tacagctacc gcgttaccga gctagaagca gctctaggcg tagcccagct
    10081 ggaaatcaag gatagtattc ttgaagcccg ccaggacaac gcaaagtatt tgatagacgg
    10141 ccttaaaccg cttgaggctg ccggtcacat tcagcttcct tcggtgccca actatgccga
    10201 acatgccttt atgcttttcc cggttgtggt taaagatcct gcgttaggca gctccatgct
    10261 aacctatcac ttggaagaac acgctatcga aacccgcccg atgatgccgc ttattaacca
    10321 acctttttac gtcgaacggt ttggaaacat tgaaggcgcg tatccaaccg ctcagtggat
    10381 aaataagggg ggcttttata tcggctgcca tcagggtttt acgcacgaag atcttgatta
    10441 catcattcaa atctttaacg acttctttcg gggcaagtaa tgagcttctg gaaagacaaa
    10501 caagttgttg ttaccggggg tcacggcttc ctgggcggtc acctggccaa acggcttaat
    10561 gccgagggcg cgaagatctc gaccccgcct cactctgagt atgatttagt taagcttgaa
    10621 gatgtgcgcc agatgtacga cgatcatccc ggcaccgaaa tggtgattca tctggctgcc
    10681 aaagtcggcg gcatcggcgc caatcgcgag catcccggct cgttctttta cgacaatttg
    10741 atgatgggcg ctcaactgat gcatgaagcg tatcttcgca aggtgccaaa gttcgtggcg
    10801 ctcggaaccg tctgcgccta ccccaagcac actcccgtcc cttttaaaga agatgatctc
    10861 tggaacggct atcccgaaga aaccaacgca ccttacgggt tagcaaaaaa aatgatgctg
    10921 gtgcaaagcc agggctatcg ccaagagtat gggtttaatt ccatcttcct gctgccggtt
    10981 aacctttatg gaccaggcga caactttgat ccggcaacct cgcatgttat accggcgctt
    11041 attaagaaat gcgtagacgc tattacgagt aacaccgagg aaattgtggt atggggggat
    11101 ggctcgccta cccgcgagtt tttgtacgtt gaagatgcgg ttcaggctat cttgctggct
    11161 gctgaaaagt acaatagcag cgagccggtt aacctggggt cgtcctttga agtatcgatc
    11221 aaagaactgg tcgaaattat tgccaaagag accggcttta acggcaaaat tgtatgggac
    11281 gccagcaagc cgaacggcca gcctcgccgc aagctggata catcccgtgc cgagcaagaa
    11341 tttggcttta aatcggcaac tacctttgaa gaaggactcc gcgaaacaat tagctggtac
    11401 caacggaatt cttcttaaat ggacgctctc acaaagaaga ctgccagaaa ttcgagctac
    11461 agcactatcg cattcatttg gccgatactc ctagcttttt ttgtaacgcc ttttattgtt
    11521 cacgctttgg ggaccgaaca gtacggaatc tactcgctgg taattgcctt tgttggattc
    11581 ttcggattcc ttgatctggg aattgcccct tcgttggtga agtatgtctc ggaatacaac
    11641 gcccgcaagg acgataatgc cattaacctt ctcttcaact cggcactatt tctctacctt
    11701 actattggga tcatggcggc atttttaatt gccggatttg ggagatttta tgcgcccgat
    11761 gtttggaaaa ccagcgctga aaatgcccca ctcctccaaa ctgttttgta catcgccgcc
    11821 attggttttc tcattaatat ggtgctaacc gccttttctg cccttcccag cgcccttcaa
    11881 cgctttgaca tcaccagcaa gattaatctg gtagttacaa cgatatctac aggactaacc
    11941 gtgctcttat tgctagctgg ggaaggttta gtggcaatcg tcgcccttgg catatttacc
    12001 agcttggccg cgctgctcgt ttaccacttt attaaccgac gcctactccc ggcattgcgt
    12061 ctaaagcctc atattgaccg taagatgctg aaactggttc tggtgtttgg cggctatgcc
    12121 accattggca cgctggcctc ggtaatcctt ttgcagctcg acaaattcat tattggtgca
    12181 accctgggag ccagcgccgt tgccttttat gtcattccag gaaacctagc cgtaaaaatc
    12241 catgggacaa tatcggccct gacagcaatc atcttcccgc ttagctcgga actattcgca
    12301 accggaaacc tagagcggct acacagcctt tacttccgag ccactcggct ggttttaacc
    12361 tttattaccg tcgcggttac accgatgtat gtgttcgcac accccttcct gctccactgg
    12421 attggagtca gctttgcaca gaacagcacg ctagtcatgc agttgctgct agctacctac
    12481 tctattctga gcctgaccgc tattcccttc tttgtcttgt atggtgcggg gaagccgaag
    12541 atggcggccc tcttttcagt tatctcgggg gttctgaatg ttgcgcttat tcttatacta
    12601 atcccccgct tcggtattaa cggggcagct tttgcttatt tgctatcggt gctgccctgc
    12661 attctgtttg tacgctacgt cgaaaagaag gtattacggt tgtcggtcag ggcgttttat
    12721 gtcggcatcg gtccaaggtt aatggtacta ttcggggctt c
//
LOCUS       DASVGB010000134         5759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_95992, whole genome shotgun sequence.
ACCESSION   DASVGB010000134 DASVGB010000000
VERSION     DASVGB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95992"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VF272_04150"
     CDS             <1..517
                     /locus_tag="VF272_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX7260093.1"
                     /translation="FIFAHFINPHSPYVFTKDGGVPTYRGDENDLDIPRRDKYVNQVE
                     YVNQTIGHLVADIKANATKPPIIIIQADEGPYPMKQPSAWQTASADTLQLKFGSLAAY
                     YLPDASPEESANVTSSVNIFRFVFNRYFGTSMPYLPDCSFVFNNTDRPFKFYAITSEF
                     HPEDPECQRYN"
     gene            538..1710
                     /locus_tag="VF272_04155"
     CDS             538..1710
                     /locus_tag="VF272_04155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX7260094.1"
                     /translation="MNALDIAIIVILLADVVRGARIGFVRQFFSFAGFWGGLFIAALF
                     APLASRFGSTPLEKISLILAAALVIGLTLSALGEYIAKYLSQLVKRFKIHPLDASLGA
                     AFGAILVGLSVWLVSAMLFNVPSKALSDQVHGSKIVRFIGQVLPPAPPVIARIQSLIN
                     PAGFPQVFLGPEPKPGFVEGGATQAEVDQAVAADGLSTVKITGKGCGGELVGSGFVAA
                     NGLVVTNAHVVAGITEPLILDRTGVHRAVVVAFDPNLDAAVLRAGGLAGPVLPLQTSL
                     VANGTHSVALGYPGGGPLAAAPAAVIQSYLATGRNIYAEGITTRRVYEIKADIHPGNS
                     GGPLVLPSGEVIGVIFARSQTNDQIGYALTAKSVVPLLERAATSRRAISTGVCAAE"
     gene            1731..2153
                     /gene="msrB"
                     /locus_tag="VF272_04160"
     CDS             1731..2153
                     /gene="msrB"
                     /locus_tag="VF272_04160"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004059696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7260095.1"
                     /translation="MTFPSKLTDDELRKKLTPEQYAVVRERATEPAFSGEYLDNQKNG
                     MYRCVACGIPLFSSDTKYESHSGWPSFWKSVDDSTITLVPDDSQGLHRIEAVCSSCGA
                     HLGHVFEDGPQPTGQRYCINSLALDFEADRTKKSEIQP"
     gene            2153..3547
                     /gene="zwf"
                     /locus_tag="VF272_04165"
     CDS             2153..3547
                     /gene="zwf"
                     /locus_tag="VF272_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7260096.1"
                     /translation="MKQQPISLTIFGISGDLASRKLLPALYWLLAAGELPKHMIIIGV
                     SRQEISVDRLMKKVAVYTGKKADPEVIASLGAMIRMVRMDSGHVDEYANLKKVYKSIE
                     TTDEAVRLLYLALPPRVSRPVIAAMAEAGITKKAHKDIRLLLEKPFGFNLASAKALDA
                     NLGEAVHEKQIYRIDHYLAKETAQNITVFRFANPIISAVWNNRVIERIEIEASETIGI
                     ENRANFYEQTGALRDVVQSHLLSLLALVLMEEPKAGDVADFHRKRRRVLESIKPFSSK
                     DVTVRALRGQYTTYRKEVGNPDSLTETWAALQLESKSRRWKGVPLYLSGGKALDSRRT
                     SIRVFLKASARQHYNCLTLRLQPREGIELQLEAKREGLTDATEPIFLKFAYGASAAGR
                     RPDGYERVLLDAIKGDRASFLTGPEILATWRLVEPVLQAWQRDNAGLHHYKPGSKPPL
                     ASLHDERGRIIPSS"
     gene            3587..4909
                     /gene="aspS"
                     /locus_tag="VF272_04170"
     CDS             3587..4909
                     /gene="aspS"
                     /locus_tag="VF272_04170"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX7260097.1"
                     /translation="MSKSKIIAIADVANHVDENVTVRGWVQTIRDQSKIKFMIIRDVS
                     GTVQSVLLTSAKQPFELSADITTESVIALTGKAVAAKQAPGGVEIVAEHIEALSLADP
                     QLPIQVIDKNEEEADLNTRLDWRWIDLRKPEKQLLFKTWTVMDQAFHNHLVDNGYIQI
                     YSPKLINTASESGAEVFEVAYFDRKAYLAQSPQFYKQMAMASGLEKVFEAGPVFRAEP
                     SFTTRHSTEFTGYDLELAYVDSHQDVMEVQEELLVAMMQAAKKAYGPAISEHYGERVV
                     VPSRPFPQVTLQEAKEMLQAAGVKSEKADDLNPEEERELSKLIQARDGHEFVFVTDYP
                     VTARPFYHMRYEDKPGLTKSYDLLFRGIEITTGAQREHRHEVLIAQAQEKHMDLDSLS
                     FYFNFFKYGCPPHGGLGMGPNRLLMRLFNVPSLREAMYVYRGPHRLVP"
     gene            4939..5340
                     /locus_tag="VF272_04175"
     CDS             4939..5340
                     /locus_tag="VF272_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260098.1"
                     /translation="MAILLLIPFTASADDASILGPQGSLAPATTAATDSSSPQTGSIL
                     QPAGASNSPIQSAGAQAGGIIVPQSQTLQQTGPADQIKVLLAGEGEGQQPLQQERNYS
                     WLIAIAGVIAATIIITAPLVWLERRSYISTR"
     gene            5377..>5759
                     /locus_tag="VF272_04180"
     CDS             5377..>5759
                     /locus_tag="VF272_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260099.1"
                     /translation="MKCQHCGSKDIISIQGQNFCLNCGVEVIAPPVSAPKAPKSKKTA
                     HFPAANAGYKPAAKPQVSQASSLDHPKSKKSGQKSSSVHPLRFAIKVASSLALLAGTL
                     IWASLWFKLDNDLVLYLSASGLAVLL"
BASE COUNT         1404 a         1409 c         1471 g         1475 t
ORIGIN      
        1 gtttattttt gctcatttta tcaacccgca ctccccctat gtattcacca aagatggtgg
       61 cgttcccacc tataggggcg atgaaaatga tctcgacatt ccgcgtcgtg ataagtacgt
      121 aaatcaagta gagtatgtga atcaaacaat tggccacctt gttgctgaca ttaaggctaa
      181 tgctacgaag ccgccgatta ttattattca ggccgatgaa gggccctatc ccatgaagca
      241 gccgtcagct tggcagaccg cttcagctga taccctacag cttaaatttg gctccctggc
      301 tgcctattat ttgccggatg catctcccga agagagcgca aatgtgacct catcggtaaa
      361 tattttccgc tttgttttca atcgttactt tgggaccagt atgccctatt taccggattg
      421 ctcgtttgtt ttcaataaca ccgaccggcc attcaagttt tatgctatta cctccgagtt
      481 tcatccagaa gatccggaat gccaacgcta taactaattt caggtatcat agaccctatg
      541 aatgcgcttg atatcgccat tattgttatt cttttagccg atgtggtccg cggtgctcgg
      601 atcgggtttg tgcggcaatt ttttagcttt gccgggtttt ggggagggct ctttattgct
      661 gctctgtttg ctccgctggc gtctcgcttt ggatcaacac cgctagaaaa aatttccctg
      721 atcctggcag ctgctttggt cattggtctc accttgagcg cgcttggcga gtatatcgct
      781 aaatatttga gccagctggt gaagcgattt aagattcatc cgcttgacgc cagtttaggt
      841 gctgcatttg gagcaatcct ggttggttta tcggtctggc tggtttcggc aatgctcttt
      901 aatgtaccga gcaaagcgct gagcgaccaa gtgcacggtt caaaaattgt gagatttatt
      961 ggacaggtgc tgccgccggc cccgccggtt atcgcaagga ttcagagcct tatcaatccg
     1021 gctggctttc cgcaagtatt tcttggtccg gagcctaagc caggatttgt tgaaggcggc
     1081 gcgacccagg cggaggtaga ccaggcagtt gcagccgatg gcctttctac ggtaaaaata
     1141 accggtaaag gctgcggcgg tgaactggta ggttcgggtt ttgtggccgc gaacggattg
     1201 gtcgtgacca acgcccatgt ggtcgctggt attaccgagc ccttgatctt ggatcgcact
     1261 ggagtgcacc gagcagtggt tgtggcgttt gatccgaatc tggatgcggc ggtattacgt
     1321 gctggaggtc tggctggccc ggtgttgccg ttgcaaacaa gcctggtggc caatggtaca
     1381 catagcgttg ccttgggcta tcctgggggc gggccgctgg ctgccgcacc ggcagccgtc
     1441 attcagagct acttggcaac cggaagaaac atttacgctg agggaattac cacccgccga
     1501 gtatacgaga ttaaagccga tattcatcct ggtaattccg gcgggccgtt agttctgcca
     1561 agcggcgaag ttattggagt gatatttgcc cgttcccaaa ccaacgatca gattggctac
     1621 gctcttaccg ctaaatcggt tgttccgtta cttgagcggg ctgcaaccag tcgacgggcg
     1681 atttcaaccg gtgtctgtgc tgcagagtaa gctggtataa tcaaaatcat atgacctttc
     1741 cttctaaact gaccgatgac gagctgcgaa aaaagctaac tccggagcaa tacgccgtgg
     1801 tgcgggagcg tgctacggaa ccggcgttct ccggtgaata ccttgataac caaaaaaacg
     1861 gtatgtaccg ctgtgtggcc tgcgggatac ctttattttc ctcggatacc aagtatgaat
     1921 ctcattccgg gtggccgagc ttctggaaat cggttgatga cagtactatt accttagttc
     1981 ccgatgatag ccagggcctg caccgtattg aagcggtttg cagctcctgt ggcgcccatt
     2041 tgggccatgt atttgaagat ggtccgcagc caaccggtca acgttactgt attaattcgc
     2101 tagcattaga ctttgaggcc gatcgcacca aaaaaagcga gattcagccc taatgaagca
     2161 gcagccgatt tccttgacga tttttggaat ttccggcgat ctggcatcaa gaaagctgct
     2221 tccggcactg tattggctat tagctgccgg agagctgcca aaacacatga ttattatcgg
     2281 cgtttcacgc caagaaatca gcgttgaccg gttaatgaaa aaagttgcgg tttacaccgg
     2341 caagaaggct gatcctgagg ttattgctag cctcggagcg atgatccgca tggtccgcat
     2401 ggatagcggc cacgttgatg agtatgccaa ccttaagaag gtttataagt caattgaaac
     2461 taccgatgag gcggtgcgat tgctgtatct tgcactaccg cctagagtct cccgtccggt
     2521 gattgcggcc atggctgaag ccggtataac caaaaaagca cataaagata ttcgtttact
     2581 gcttgaaaaa cccttcggat ttaacttggc ttctgcaaaa gccctggacg ccaatctagg
     2641 tgaagcggtg catgaaaaac aaatttatcg cattgatcac tatttagcga aagaaaccgc
     2701 ccagaacatt acggttttcc gcttcgccaa cccgattatc tcggccgttt ggaataatcg
     2761 cgttattgag cgcattgaaa tcgaagcaag tgaaactatt ggtattgaaa atcgggctaa
     2821 tttttatgaa cagaccggtg ctttacgtga tgtggtgcaa agtcatttat tgtcattatt
     2881 agccttagtg ctcatggaag agccaaaagc cggagacgta gccgactttc atcgtaagcg
     2941 ccggcgggtg cttgagtcaa tcaagccatt tagttccaag gacgtaaccg tgagggcact
     3001 gcgcggacag tatacaacct accgtaagga ggttgggaat cccgactcct taactgaaac
     3061 ctgggcagcg ctgcaacttg agtccaaatc caggcgctgg aaaggtgttc cactctactt
     3121 aagcggcggc aaggccttgg atagccgccg tacctcaatt cgcgtatttt tgaaggcttc
     3181 ggcacggcag cactacaatt gtctgacatt acgtttacag cctcgcgaag gcatagaact
     3241 gcaattggag gccaagcgtg aggggctgac cgatgcaacc gagccaattt tcttaaagtt
     3301 cgcgtatggt gcttcggccg ccggtcgccg accagacgga tatgaacgag tattgctgga
     3361 tgcaatcaag ggcgaccgag ctagcttttt aaccgggccg gaaattctcg ccacctggag
     3421 actggtggag ccagtactgc aagcttggca gcgcgataac gcaggactgc accattataa
     3481 acccggctca aagccgcctt tagcctcgct tcatgatgag cgcggccgta ttatcccttc
     3541 atcctaaacc tcgacgctga tccttaaata cactattatg tgtgccatga gtaagtccaa
     3601 aattattgcc attgccgacg ttgccaacca tgtcgatgaa aatgtaacgg ttcgcggatg
     3661 ggtgcaaacg attcgcgatc aaagcaaaat caaatttatg ataatccgag atgtcagcgg
     3721 tacggtgcag tctgtgttgt taacctctgc taagcagccg tttgaacttt cagccgacat
     3781 aaccaccgag tcggtgatag cactcaccgg taaggcagtg gcagcaaagc aggctcctgg
     3841 cggcgttgaa attgtagccg agcatattga ggcgctgtca ctggctgatc ctcaactgcc
     3901 gatacaggtt attgataaaa acgaagaaga ggccgattta aacactcggc ttgactggcg
     3961 ctggatcgat ctgcgaaagc cggaaaaaca gctgctgttt aaaacctgga cggttatgga
     4021 ccaagccttt cataatcatt tggttgataa cgggtatatt caaatctatt cgcccaagct
     4081 gattaatacc gccagtgaat ccggagccga agtttttgag gtggcctatt tcgaccgcaa
     4141 ggcatacttg gcgcagtcgc cccagtttta taagcagatg gctatggctt ccggtctgga
     4201 aaaggtattt gaagccggtc cagtgttccg cgctgaacct tcttttacca cgcgccattc
     4261 aaccgaattt accggctatg acctagaact tgcgtatgtt gactctcacc aagatgtaat
     4321 ggaagtacag gaagagctgc ttgtcgccat gatgcaagcg gctaagaaag catacggccc
     4381 ggctatttcc gaacattacg gggaacgtgt ggtggttccg tcacgaccgt ttccgcaggt
     4441 taccctgcag gaagccaagg aaatgcttca ggcagctggc gtaaaaagcg agaaggctga
     4501 tgatttgaac cccgaggaag agcgggagct ttcaaaactc atacaagcac gcgatggcca
     4561 tgagtttgtg tttgtaaccg attatccggt aacagcccgt ccgttttacc acatgcgtta
     4621 cgaagataaa ccggggctga cgaaaagtta tgacttactt tttcgcggca ttgaaattac
     4681 caccggcgct cagcgcgaac atcggcatga ggtgctaata gcccaagcac aggaaaagca
     4741 catggatctt gattcattat cgttctattt caatttcttt aaatatggat gtcccccgca
     4801 tggcggtctt ggtatggggc cgaaccgttt gctgatgcgc ctgtttaacg taccaagctt
     4861 gcgtgaagcc atgtatgtgt accgcggccc tcacaggttg gtgccctaac gatgctcgct
     4921 atacgccgtc ttatcgcaat ggctatcttg ctgttgattc cgtttacggc gtcggctgat
     4981 gacgctagca tcttagggcc acagggatca ttggcaccag ctaccacggc tgccaccgat
     5041 tcgtcttcac ctcaaaccgg cagtattttg caaccggccg gtgcatcaaa ctcaccgatt
     5101 cagtcggccg gcgctcaagc gggaggtatt atcgtaccgc agagccaaac ccttcagcag
     5161 accggtccag ccgatcaaat caaagtactt ttagccggtg aaggcgaggg tcagcaaccg
     5221 ctgcaacagg agcggaacta ttcatggttg attgcaatcg caggagttat tgctgcaaca
     5281 attatcataa cggccccctt agtttggttg gaacgccgca gctatatatc aacacggtaa
     5341 ttgtgcttgt gcttattcga tgcttaaata taagtaatga agtgtcaaca ttgcggctcg
     5401 aaagacataa taagtattca agggcaaaat ttctgcctta attgcggtgt agaggtgatt
     5461 gccccgcctg tttcggctcc aaaggcgcct aagtcaaaaa agacagcaca ttttccagct
     5521 gccaatgccg gttacaagcc ggccgctaag cctcaggttt cccaagcatc gtcgcttgat
     5581 catccaaaat caaaaaaaag cggccaaaag tcatcttcgg ttcatcccct gcggttcgca
     5641 attaaagtgg cttcaagttt agcacttttg gctggcaccc tcatctgggc atcattatgg
     5701 tttaagcttg ataatgacct cgtactctac ctatcagcaa gtggtctagc ggtattgct
//
LOCUS       DASVGB010000135         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_667056, whole genome shotgun sequence.
ACCESSION   DASVGB010000135 DASVGB010000000
VERSION     DASVGB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667056"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2205)
                     /locus_tag="VF272_04185"
     CDS             complement(<1..2205)
                     /locus_tag="VF272_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7260100.1"
                     /translation="MSAIAYTPSSFRALKARFAARLGKRSLKVEAGLILILAMGGSYA
                     IISGYRQGVGLLGLGLAALIFSLWHRWDLMRLAPPANAWQVQPLHLDLVVEQTLLARL
                     KPGMSADQVWLAACLQWEAAFVLQRLAMPATVISDILAKTTVQSDAIWQTASELAHTE
                     SGEEIDSAAVVVAVLTTVPEIQPVLAHLKRTNEQVLSVFAWQQRAKRSIKGLSQAPYF
                     GGIGRDWASGYTPLLSRFGTNLSRDIEQGYYRTIPSLHEPLIHQLTELLDKRHPVALI
                     GGVGSGKTALIYSLAERILKPEAVGGLNYFQVVSLNASELIASGAALEDIMLRLLGEA
                     AAARNSIIFLDEAQLFLNAGSGSVDITQILLPVLKNSSVPLILAMSDHDWQGLTARNP
                     GFTGQLQRLTLIEPSEPEVVAVVQDAAIGIEHQAGAIIQHPAVLEAVRLAGRYLPELA
                     FPGKAITVLESASSYVQQGFITADSVQRAVEATTGAKLTSASAPERQQLLNLEDQIHS
                     RMVNQVRAVKVVADALRRSRAGVGSAKRPVGSFLFLGPTGVGKTELAKALAVSYFGGV
                     EAMVRLDMSEYQQPSDVSRLLAPSSDSQAGQTLASGVRQRPSTVVLLDEIEKSHPDIL
                     NLLLQLLDEGRLTDSDGRSVSFRDAIVICTSNAGADEIRQRIAEGQQLENFESMITDQ
                     LIKEHTFKPELLNRFDEIVVFRPLTKAELHQVVELMLGEVNASLKSQNITIKV"
     gene            complement(2202..2564)
                     /locus_tag="VF272_04190"
     CDS             complement(2202..2564)
                     /locus_tag="VF272_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260101.1"
                     /translation="MLALAFFQWWYGPGWLHLLRGIGKNCSRILQTFSVVILLRTLFA
                     PWRRIITYGNNSFIDGLKAMLDNAVSRAVGFAVRIIVIITALLLLVVIAITGIVVLIV
                     WPLLPPLAIVMIGRGLFP"
     gene            complement(2586..3956)
                     /locus_tag="VF272_04195"
     CDS             complement(2586..3956)
                     /locus_tag="VF272_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX7260102.1"
                     /translation="MSDEIVIHQAELDSSPSPYGPRPSSGHHWRRALKWLGLTVLVLG
                     AVAALYVAFNITKISANPFGFGKLKGEELGRINIMMLGVGDPGHAGENLSDTNLLISV
                     DSRSHAIAIISIPRDTRVKIPGYGSGKINTAHAKGGIPTAKDTMETNLGVPVHYFVKA
                     NFSGLRQVVDAVGGVDVTNTTRLNDPEYPCDNNQYKRCGFKLAPGKHHLNGATTLKYV
                     RCRKGTCGDDFGRAQRQQEVMQAIRQKVTSAGTLANPIALGRLVAAAGDNIQTDLSVN
                     NVLRLNELTKKTPKNNIYNVVFNLQPGGFLVADPRGSGDLVPSSIDFDDIQEFVQNVF
                     ARAPIWSEQPKLVIQNGTTTPGLGGKFSGKLRLDGKDVTVQAVGNALTSDHTTSLIVD
                     NSRGKKPHTIAYLERLLNVKAAIPGTSGAIAGGDVVVVLGSDYASSIGAVSPGSASPA
                     ANTSPR"
     gene            4058..>4673
                     /locus_tag="VF272_04200"
     CDS             4058..>4673
                     /locus_tag="VF272_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7260103.1"
                     /translation="MNEERAKSEQEAASTAAASGIPYSDTRELAFPTEMPEGITRELL
                     EEYRVAPLHLYNPQSLELGLTGKTDRTKLPELQAKAPSVKLTFKTISDPGYQTISNQL
                     FYQEFTKEREGDFATFGKKLAIAAPKQAFQDIAQLAFWLGSSDIHIEPQTDSARIRFR
                     IDGTLHHITNVAIQAYKVFLSDLQTKAEVTWGSDKPQSGRISFEL"
BASE COUNT         1159 a         1284 c         1169 g         1061 t
ORIGIN      
        1 tactttaatt gttatgttct ggcttttaag cgatgcatta acctcgccca gcattaattc
       61 cacaacctgg tgcaattcag ccttggtcaa tgggcggaat accacaatct cgtcgaaacg
      121 gttcaaaagc tcgggtttaa aggtatgctc ctttataagc tgatcggtta tcatgctttc
      181 aaagttttcc aactgctgtc cctcggcaat gcgctgtcgg atctcatcgg caccggcatt
      241 cgaggtgcag ataacaatcg catcccggaa ggataccgaa cggccatcgc tatccgttaa
      301 ccgaccctca tccagcagct gcaacaagag attgaggatg tcgggatggc tcttttcgat
      361 ctcatcaagc aatactacgg tcgaaggacg ctgtcgtacg cccgaagcaa gcgtctgacc
      421 tgcctgggag tcgctggagg gcgcaagcag cctgctgaca tcgctaggct gctgatactc
      481 gctcatatca aggcgtacca ttgcctccac cccgccgaag taactgacag ccagggcttt
      541 ggccagttcg gtctttccaa cgccagtagg tcccagaaat aaaaagctgc cgactggtcg
      601 tttagcgctg ccgacaccag cgcgcgaccg gcgcaaggca tcggctacca ccttaaccgc
      661 ccgaacctgg tttaccatgc ggctatgaat ctggtcttcg agatttagga gctgttggcg
      721 ctctggggcg ctggcagagg ttaatttggc accggtggta gcttctaccg cccgctgcac
      781 cgaatcggcc gtaataaagc cctgttgaac gtacgaagaa gccgactcca gcacggtgat
      841 ggctttgccg gggaatgcca gctcgggaag atagcggccg gccaatcgaa ccgcctccag
      901 gaccgccgga tgctggatga tagcccccgc ctggtgctca atgccaatag ccgcatcttg
      961 aaccaccgcc accacttcag gttcgctcgg ttcgataagc gtaagccgct gaagctggcc
     1021 ggtaaagccg gggttgcggg cagttaatcc ctgccaatca tgatcgctca tcgccaaaat
     1081 taggggaacg ctactgttct ttaatactgg caacagaatt tgggtaatat ccaccgaccc
     1141 cgagccggca tttagaaaga gctgggcttc atccaggaaa ataatactgt tgcgggcggc
     1201 agcagcctct cccagcaagc gcagcatgat atcttccaaa gctgcacctg aagcaatcag
     1261 ttcgctggca ttcaatgaca caacttggaa atagttgagg ccaccgaccg cttcaggctt
     1321 taaaatccgt tcggccagtg aatagataag cgcggttttg ccgctgccga caccgccgat
     1381 cagcgctacc ggatgtcgtt tatctagaag ctctgtaagc tgatgaatta agggttcgtg
     1441 caggctggga atagttcggt agtagccttg ctcgatgtcc cggctgagat tggtgccaaa
     1501 ccgagataat aaaggcgtat agccgcttgc ccaatcgcga ccaatgccgc caaaatatgg
     1561 tgcctggctc aaacctttta tgctgcgctt agcccgctgc tgccatgcaa acaccgacag
     1621 aacctgttcg ttggtacgtt taaggtgagc taataccggt tgaatttctg gtacggtagt
     1681 aagcacggcg actacgaccg ctgcactatc gatctcttcc ccgctctctg tgtgggccag
     1741 ttctgaagcg gtttgccaga tcgcatccga ttgaacggtg gtcttcgcaa gaatatcgct
     1801 aattaccgta gccggcatcg ccaaccgttg caagacaaac gctgcctccc actgtaaaca
     1861 ggccgccagc catacttgat cggcgctcat ccccggtttc aaccgcgcca acaaggtttg
     1921 ctcgactact agatcgagat ggaggggttg aacctgccag gcgtttgccg gcggtgcaag
     1981 cctcatgaga tcccagcggt gccataacga gaaaattaac gcggctaaac caagaccaag
     2041 cagaccaacc ccctgccgat atccgctgat gattgcatac gacccgccca tcgccagaat
     2101 cagaatcaga ccggcctcta cctttaagct ccgctttccc aagcgggcag cgaagcgagc
     2161 ctttaaagct ctaaagctgc taggggtata ggcaatagcg ctcacgggaa aagcccccgg
     2221 ccaatcatga ctatggcaag cggcggcaaa agcggccaga caataagcac tacaatgcca
     2281 gtaatagcga tgacaaccag cagtaaaagc gctgttatga ttacaatgat gcggactgcg
     2341 aagccaactg cccgggaaac ggcgttatca agcatagcct tgagtccgtc gatgaatgaa
     2401 ttattgccat acgtaataat cctcctccag ggtgcaaata gtgtccgcag taaaattacc
     2461 actgaaaagg tttgaagaat tcgggagcag ttctttccaa tccctcgaag cagatgcagc
     2521 cacccgggac cgtaccacca ctggaaaaac gctaatgcta acatggcctt attgtagcat
     2581 gagatctatc taggggaggt gtttgcggca ggtgaagccg agcccggtga aaccgcgccg
     2641 atacttgaag cataatcgct tcccagtacc accaccacat caccgccggc tatagcaccg
     2701 gaggttcctg gtatcgcagc cttcacgttt aacagtcgct ccagatacgc gatggtgtgt
     2761 ggttttttac cgcggctatt atcaacaata aggctggtcg tatggtcgct ggtaagcgca
     2821 ttgcccaccg cctgaaccgt tacgtcttta ccgtcgaggc gaagttttcc gctgaatttt
     2881 ccgcctaggc caggcgtggt ggtaccgttc tgaatgacca gtttcggctg ctcactccat
     2941 ataggcgctc gggcaaacac attctgtacg aactcctgaa tatcatcaaa atcgatcgag
     3001 gaaggcacca ggtcgccgga gccccgggga tctgcaacca gaaaccctcc cggctgcaga
     3061 ttaaacacaa cgttgtagat attatttttc ggagttttct tggttaattc attgagccga
     3121 agcacgttgt ttacactgag atcggtctga atgttgtcgc cagcggctgc cactagccgt
     3181 cccaatgcaa tcgggttcgc cagggtgccg gccgaggtaa ctttctgccg aatcgcctgc
     3241 atcacttctt gttgccgttg ggcacgaccg aaatcgtcgc cgcatgtccc tttacggcag
     3301 cgaacatatt taagagtggt ggcaccatta aggtgatgct taccgggagc tagcttgaaa
     3361 ccgcaacgct tatattgatt attgtcgcaa ggatattctg ggtcattcag tcttgtggta
     3421 ttggtgacat caaccccccc aaccgcatcc actacttggc gtaagccgct gaaattagct
     3481 tttacaaaat aatgaaccgg taccccaaga ttcgtctcca tcgtgtcctt ggcggttgga
     3541 atgccgcctt tggcgtgagc cgtgttaatt tttccgcttc catatcccgg aatctttacc
     3601 cgcgtatccc gtgggatact gataatggca attgcatggc tgcgagagtc cacgcttatg
     3661 agtaagttgg tatcgctcaa attttcgccg gcatggcccg gatcacctac gccgagcatc
     3721 ataatattga tgcgaccaag ctcctcccct tttaatttgc cgaagccaaa gggatttgcg
     3781 gaaatcttgg taatattaaa ggcgacatac agtgccgcaa ccgcaccaag gaccaaaaca
     3841 gttagaccca gccattttaa cgcacggcgc caatggtgcc cggaagaagg ccgaggccca
     3901 tacggtgagg gcgagctgtc aagttcagct tgatggataa caatttcgtc gctcataccg
     3961 aaacattgta ccaagccggc agcataagct ccatagtcta aacaggtgac gaggatgctg
     4021 atatgcctct aattctgctt atgctaaagt taagggtatg aatgaagagc gtgcaaaaag
     4081 cgagcaggaa gcagcatcga cagcggcggc cagcggcatc ccctactccg acacccgtga
     4141 gctggctttt ccaaccgaga tgcccgaagg tataactcga gaattattag aagaatatcg
     4201 cgtagcaccg ctgcatctct ataatccgca gagcttagag ctagggctta ccggcaaaac
     4261 cgaccgaacc aagctgcctg agctccaggc taaagcgcct agcgttaagc tgacttttaa
     4321 aaccatttct gatcccggct atcaaaccat tagcaatcag ttgttttacc aagagtttac
     4381 caaagaacgc gaaggcgact ttgctacctt cggcaaaaag ctcgcaattg ccgctccaaa
     4441 acaggctttt caggacatag cccagctggc attctggcta ggttcttcag acattcacat
     4501 tgagcctcaa accgatagtg ccaggatccg ctttcgcatt gacggcaccc tgcaccacat
     4561 taccaacgta gccattcagg catataaagt ttttttgagc gatctgcaga ccaaagccga
     4621 ggttacctgg ggctctgata aaccccaaag cggccgcatt agtttcgagt tga
//
LOCUS       DASVGB010000136        17772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_753633, whole genome shotgun sequence.
ACCESSION   DASVGB010000136 DASVGB010000000
VERSION     DASVGB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 17772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17772
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753633"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1323
                     /locus_tag="VF272_04205"
     CDS             88..1323
                     /locus_tag="VF272_04205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7260104.1"
                     /translation="MKIAIVVWDLSVGGGTQRQALELARALTPHHEVTLYCYAFNPET
                     CYPEITKGLKVISVAEKVPQYPAPQNLGKISQVFYTGYQIFHLADNEMRKLEELIGPD
                     YDVVNFHDYYTEAVGYHYKHRHPNVRTVLMSNDVSYVKMTEEWRQNIPKTSSVLGSLA
                     VKTKDLLLALEKRRQAKLIRSFNKVVVLDHRNQDLLRGYFGIEAVVIRSGLDVNHFKP
                     QRAEFNKVPVILGAGILFRWRRFEDLISAAAIVRDAGKRFKLRIIGSEDYDPLYSSEL
                     HQLVTELSLQDHVAFLGRVSEDTLLRNYQQSDVFVFPNHNQTWGLAVFEAMASGTPVI
                     VSRTSGAHEVLTDKVNALLVDPLHPEQIAQKILGLLEDPKLREELARSGREFVEHNIS
                     WKLYSTNMLNAFHGSSNNE"
     gene            1316..2086
                     /locus_tag="VF272_04210"
     CDS             1316..2086
                     /locus_tag="VF272_04210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7260105.1"
                     /translation="MSKPLFSIITVCFNSAHEIERTLKSLEKQVNRDYQYIVIDGKST
                     DTTMAMIDKYKHLVTKLVSEPDKGVYDAMNKGLALAEGELIFFLNAGDELYENTTLQK
                     VAADYQKAGRPEVIYGDIIEQPLKGDAYVRKFPKISYGYLYRDTICHQALFAQACVFE
                     YTGPFNLKYSVCADFDWLLNAYANRRFRIHQIDEVMSIFYLGGMSSKVNGMAERRSII
                     LKHMPLNARVPRDFYAAGFSLTKRIKNKLRRTLWPKTP"
     gene            2068..3048
                     /locus_tag="VF272_04215"
     CDS             2068..3048
                     /locus_tag="VF272_04215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7260106.1"
                     /translation="MAKNTVSVLVITYNQKGFIAQWVESLRAQTTPPDEVVIVDDSST
                     DGTYEQLQAISKKHGYTLMRTPRNSGPSAARNIGLQSVHGDFICFLDGDDYFLPEKIT
                     VQKKALEKNPNAVLCYGDCLIDKQGTILPFNLSALMPLNEGALFDQLLSRNSMLLGGV
                     MLRSRLAGNHRFDETLMRAEDYMYFLEISKKNDTFALVKEPVFVYRRANAGLSSQRLE
                     TIACNEEVLRRVAGWALPKPQVQLLNDQKTIILAEKVDLLLRSGGAGSLAALRELESL
                     SPSRSRQWLLRLLHYNSFIGRMAYVLFEFYRSLRHPRKNQDELMMEQFTR"
     gene            3045..4190
                     /locus_tag="VF272_04220"
     CDS             3045..4190
                     /locus_tag="VF272_04220"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7260107.1"
                     /translation="MKIFVEGTPLFLQRTGVGQYTKNLMDVLLKLDERNHYTIFGFLF
                     IGKRFTNKPIPEGPKLSYKLVRYLPSKIFNAVVRRLFLPPLDLLALSKPDIFFFPNFV
                     RYPLIFGAKSIVVIYDLSYIVQAQFSASPNRKYLTRYVPDSVKKADHVITISQNSKKE
                     IMEHYGVPSQKITIVTPAINHQIFFPRPDKEVEAIRQKYDISKPYILYAGTLEPRKNI
                     IGILDAFAELDAGLLQTHALVLAGGKGWLDHEIHAKIKELSELDIIVTGYLPDEDLPA
                     IYSGASLFVYPSFYEGFGIPPLEAMACGVPVITSDNSSLPEVVGDAGIMIDAHSTPAL
                     TRSMQKVLTDPGLVKQLRGRGLKQAKRFNWEQSGKILLEVINTIGRA"
     gene            4190..5116
                     /locus_tag="VF272_04225"
     CDS             4190..5116
                     /locus_tag="VF272_04225"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7260108.1"
                     /translation="MVILVTGAAGFVGRHLVELLHKSGGNDVYGTALHHGLGDAIMKQ
                     VDLKDGESVKRLLSEIKPEVIYHLAAFASPALSFKQPVEAITDTLAMQINLYEASLSL
                     GISPRFLVVGSGQIYGRADATMLPLTEEAALDFASPYAVAKVGQEHLASMYAKRGIES
                     VIARPFNHIGPGQQPGYLVSDLSKQVAELEKSASNDTLRVGNLSSKRDFTDVRDIVRA
                     YCLLAEKGRRGKVYNVCSGKSTSGQDILDTLLSFSEKKIVTEQDPARMRPSDIPDLYG
                     DASKLNKDTGWKPEIGLSQTLEDTLNYWRSRV"
     gene            complement(5119..6108)
                     /gene="gmd"
                     /locus_tag="VF272_04230"
     CDS             complement(5119..6108)
                     /gene="gmd"
                     /locus_tag="VF272_04230"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7260109.1"
                     /translation="MASQKRALITGITGQDGMYLAELLLEKDYKVFGLVRGQNNPKRA
                     IIEQEIPGVELVEGDLTDSSSLIEAIETSQPDEVYNLAAISFVAFSFKQPEITGNVTG
                     LGALRLLEAIRRVGGDNNKIRFYQASTSELYGQVRETPQTELTPFHPRSPYGVAKAYA
                     HYMTVNYRESYNMHASTGILFNHESPRRGYEFVTRKVTSTVARIKLGLQKELVLGNLD
                     SQRDWGFAGDYVRGMWMMLQQDQPDDYVLATNETHAISELLDVAFAEVGIDDWKPFVK
                     QDPRFMRPAEVDLLLGDATKAKQKLGWEPEVSFKELITMMVKNDLEIEGKKAN"
     gene            6189..7481
                     /locus_tag="VF272_04235"
     CDS             6189..7481
                     /locus_tag="VF272_04235"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7260110.1"
                     /translation="MMKLKRLEHILLCGILVTLPLERLPSVDIAGSTVRISQVLGLLL
                     IAVVLPKLWRNKRDWLKPPWLFLALFNLAAFASAALSSRPDHSIKVAGFILFVSLLGH
                     TISQVVDRSRLKQYLTWAGIGVVGSCLFGLYQFIGDLAGLPYWLTGLREEYAKGGIFA
                     FPRIQSTALEPLYFSNYLLVPLAVAATYQAFRKARKTWPISFLILIVITLGLSRGAQA
                     ASACIILVVVGVAVWKGRLRPALGVLATTVAAVGVGLMLIGFASILYEGGAGKRNTAS
                     GNVKSFAKQASNINQGESAQGRALTRHLAVRAAMGYPWLGLGPGNFGVYAMEKRPDKF
                     GSADTIVNNEVLEVAAENGLMGLVSLLLFVGFAARAAVRAALSEKDSSIAVLIWALLL
                     GLGGIVLQYQLFSTLYITHIWVAIGLLLGLSRPSRLRR"
     gene            7478..8593
                     /locus_tag="VF272_04240"
     CDS             7478..8593
                     /locus_tag="VF272_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7260111.1"
                     /translation="MKRIAIDARIISTSTGRYVERLLHYLQESDQHNQYLVLMLTKDM
                     GSWRPVSPNFKIIAADFPPYSFREQFQFTLLLYRLRLDLVHFTMPNHPLMYVKKHLIT
                     IHDLTLIKYINKRREGWVKNLYKHLIKPLVFRSVLRVGVSSASHIITPTKYVMNAIQK
                     DYGVPDKRISYTHEAADALASKSLEPKPTIAKPFMLYVGNAYPYKNLKRLVKAAEEVN
                     RSKPITLVLAGKPDYFYDELKTYVTKEHIGNVRFAGFVSDAELAWLYQHATLYVFPSL
                     SEGFGLPPLEAMTYGLPVAAARASCLPEVLQDAAVYFDPHDTSSMAQVIGDTLHDAAK
                     LRSLKQAGLKHVKGFSWRRMAEQTHRIYIQSLEAKSD"
     gene            complement(8590..9042)
                     /locus_tag="VF272_04245"
     CDS             complement(8590..9042)
                     /locus_tag="VF272_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX7260112.1"
                     /translation="MSPELVESAKHYLADLLSFFEINADVHAESVDDTIKLSVDTEAG
                     GRLIGHRGETLASLQHIMNMLIRHQTTERVYIHVDIGGYRQARLEKLEEQTREAVEQV
                     RASGEEVALPMMSPAERRHIHSILTEVEGVTSESRGEGNRRRLIVRAA"
     gene            complement(9039..9926)
                     /locus_tag="VF272_04250"
     CDS             complement(9039..9926)
                     /locus_tag="VF272_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX7260113.1"
                     /translation="MFTTIFVEPLFNVLFVLYALVPGHDFGVAVILLTVLVRLALWPL
                     VTRQLHSQRVLQKLQPEVAKVRKAAGGDRQKESQMLMELYKEKGTNPFASILPLLVQL
                     PIFFALYIVLRDAVRPDEIAKLSYDFVQNLGPVADVIKQKAAFHPALFGLIDLTKPHL
                     GLAILAGLAQFYQARQLLPQTMAAGGDQAKLMKNMTYIFPVLTAGIAMTLPSALALYW
                     MFTSLVAAFQQYWVLLRDAHELEAVEDKIHPNSVVAAKKAAPAKPVLSKAQRKAAARK
                     ANAKAKTKPKAEAASGDAE"
     gene            complement(9931..10278)
                     /gene="rnpA"
                     /locus_tag="VF272_04255"
     CDS             complement(9931..10278)
                     /gene="rnpA"
                     /locus_tag="VF272_04255"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX7260114.1"
                     /translation="MLASPNRLKQPSDIARVFRRGRQGVSGPLVLKAAPSGAVASRGV
                     VVVSKKVSKRAVIRNRIRRRIGAMLAERWATVTPGYDIVITVHSNIAETPAPELAKHL
                     DEAIRRAKMIGVF"
     gene            complement(10287..10424)
                     /gene="rpmH"
                     /locus_tag="VF272_04260"
     CDS             complement(10287..10424)
                     /gene="rpmH"
                     /locus_tag="VF272_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7260115.1"
                     /translation="MPKRTYQPKKKRRMRVHGFLQRMSTRAGRLVLKRRRLKGRKQLS
                     V"
     gene            10649..12058
                     /gene="dnaA"
                     /locus_tag="VF272_04265"
     CDS             10649..12058
                     /gene="dnaA"
                     /locus_tag="VF272_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7260116.1"
                     /translation="MESRKLWQGLLGELELTMSAGNFNTWLRNTSIIERENGHVVVGV
                     PNIYTKRWLEEKCRDEITETLTRMDATVRSIEYRVVTRAATPPTRDSFISPSPAPGRP
                     VVALGTHATGPATLEPATVQSDRRNPTPRLFNPRYTFDNYVVGSSNELAYAACQAVAK
                     YPGEKYNPLFIYGGVGLGKTHLMQAVGHEILRADPSKRVRYVTSEQFTNEFLHSIQTK
                     KTRGFADLYRNLDVLIVDDIQFLGNKEKTQEEFFHTFNTLHQANKQIIMSSDKPPKAI
                     PHLEDRLRSRFEMGMAADIQRPDLETRAAIVQRKALSQGTSLSFEIVEYLAHQFQHNI
                     RELEGALTQLMAYCEVRGLAPSIGVARELNGSSSDSRRRKQVTPRLIIEKTAAFFDLL
                     PTDITGTKRDRDIVVPRQISMYLMREELELSFPKIASTVGGRDHTTAMHSVTKIERLI
                     EVDARLRNEFNQIKEQIGR"
     gene            12346..13464
                     /gene="dnaN"
                     /locus_tag="VF272_04270"
     CDS             12346..13464
                     /gene="dnaN"
                     /locus_tag="VF272_04270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7260117.1"
                     /translation="MKLSVTQENFHKALGTVGRVVSSRSSLPVLSNILLSTDTNRLRL
                     SATNLEIGVTCWIGCKVTQEGAVTSPARLLGEFVSNLPSGKLELSASEANLTITAPHY
                     ESHINGISADEFPLIPEVKADPVVTLPAALFREAIAEVVIAASPDENRPVLAGVYMYI
                     EEDNLILVATDSYRLAERHIPLGDIQTSSLDAIVPARTMQELARILADNDGDVRMYLA
                     DNQVMFQLDSIELVSRLIEGQFPNYRQIIPQEEKTVAILDTAELARLTKMASLFAREN
                     AGSIRIEVQAEGEIRIMSSASQVGENTSSAECEVAGDDGEISLNARYLSDALAAIKTK
                     QISFAMSGKLNPCALRPRSENEEEDQDDYIHIIMPLRT"
     gene            13495..14565
                     /locus_tag="VF272_04275"
     CDS             13495..14565
                     /locus_tag="VF272_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX7260118.1"
                     /translation="MSLTQLSLTNFRSYGERTVPLDASVTLVVGPNATGKTNLLESLF
                     VLATTKSFRAKDTELIGHGQEFYRIVAKQDDNTELAMAYQVKSGSAEKRVHHGKTPKS
                     LIDHLGTIRVVLFEPNDSLLIYGGPDRRRRYLDFVLTQTDKHYAKSLNLYRRTLQQRN
                     HLLADWGGNTAELFAWNIQLAQAAAEIDERRRAVVGHINGLAVESYRIIAGSAEPLEL
                     TYHGTAAAADYAASFLKLLEAGLSRDIAAGFTTIGPHRDDFSANFKDAPIASVASRGE
                     IRTVVLALKMAELAYIQDHTGSKPLLLLDDVFSELDDSRRQHLLDTLGNYQAVVTTTN
                     ADISSELGARHAIIYTTEPHHG"
     gene            14558..14803
                     /locus_tag="VF272_04280"
     CDS             14558..14803
                     /locus_tag="VF272_04280"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX7260119.1"
                     /translation="MDSMKDSLRKRADELRLGRADELAEIQAYLDERYAGQCRAASLN
                     DGVLKITTPNASLASELRMGQVAIAQKFKVRIIITIM"
     gene            complement(14813..15259)
                     /locus_tag="VF272_04285"
     CDS             complement(14813..15259)
                     /locus_tag="VF272_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260120.1"
                     /translation="MNKRLLLIVGIGAAIVAVVVVLIIVFYPRQEDYAPNSFNPNTEE
                     ERGNPTGELGLLVVGEEKIADVVLPEQYVAVKAALEKLRDTKLSDVKKFEVQGDVVVE
                     NNGVLKLTIVADGQAPFDISIDRSIYNQIKLAAPSHSYVSIEPVQY"
     gene            complement(15256..17220)
                     /locus_tag="VF272_04290"
     CDS             complement(15256..17220)
                     /locus_tag="VF272_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7260121.1"
                     /translation="MKSLIQFSLVIALLLSSSPLQAKSGPDTPFYDGAVVCGEKVEGA
                     AGQGPLLGPFFPKVSDIGDMQAKIKSYIQKSFPNSPLLENTENLVLLGEKYNVNPAMA
                     VAFAQKETGMGTAGFGPAPQNNIFNIRNGAAGSFGSYTSVKDSIEKYYQLISGELYLG
                     KPSNFTTVKQILNRYAPPVENKTSDYIQFVNSTMQKILGGSQTTVQAETAGSQSLRGI
                     ARDNDVHSVVVQYLGQDVLEAYNEGDPPNTPASMLKLIIVDAVLNTSGVNLDDEITAT
                     SAVLYGGANGDRLAVGDVVTVRDAITAALARSSNGHANMLIRQAGGFAAITAMAHSLG
                     YTNTDINRYFKDAPSGPANVTTASNIARAMHKLYTNDRAGYEVAQAALRDSTQTWGLN
                     SEANKWGLASGASTGNPVTANSGVFPTPAGKVVITVYINKADAGPQIKKTTEDILNYL
                     KKKLAVDAESAIDCVDGEESAGAGGTAGAFGYDLTGPNKMVNFDQTDPRWASKPYGTG
                     KSSIGASGCGITSLAMIVRTLKGGTETPLTLANKYGDEYHTFGTAWSLFPVFAEDYNL
                     TMRDIGLNLDEAEKTIRNGGLVLISVDPGYFTGAGHFMVLRKVSESGDSFYLADPNGK
                     GIHGDSETRAFSRSFLQGQGALKHLWAFEK"
     gene            complement(17217..>17772)
                     /locus_tag="VF272_04295"
     CDS             complement(17217..>17772)
                     /locus_tag="VF272_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260122.1"
                     /translation="KKGRDGTTIEMPQECESRLNPPEAVNDPETEAADAGGDVAGADA
                     ELRERLLANDRLKLLRPCQKTDITSGAAKETLIRLMVAVVENAQINLPITAFKCDHDP
                     GTLHERGLAIDIGYFAGSAEGNELYKFLYANRTKFKIDELIFNPPAPGTQCVGGGKTV
                     NCVGYYGSDTMAAHGNHIHVGTFP"
BASE COUNT         4237 a         4749 c         4443 g         4343 t
ORIGIN      
        1 atcaagcggc gcccttttct tagtgctgcg gctagcagac ccgcaagatg tcaaaattct
       61 taaaagtgcc tggagaaaaa taaagcggtg aaaattgcta tcgtggtttg ggatttaagc
      121 gtgggcggcg gcacccagcg acaggccctg gagctagccc gagcgctcac tccccaccat
      181 gaagtaacgc tctattgtta tgccttcaat cccgagacat gctaccccga aatcacaaaa
      241 gggcttaagg tgataagcgt agccgagaaa gtaccccagt atccagcacc ccaaaatctc
      301 ggcaaaatat ctcaggtttt ttataccggc tatcaaattt ttcatctggc tgataatgag
      361 atgcgaaagc tcgaagagct gattgggccg gattacgatg tagtaaactt ccatgattac
      421 tataccgaag ccgttggcta ccactataag caccgtcatc caaacgtaag gacggtttta
      481 atgtcgaacg acgtctcata cgtcaaaatg acggaagaat ggagacaaaa cattccgaaa
      541 accagctcgg ttctcggctc tcttgccgtc aaaaccaaag acctcttgtt ggcactcgaa
      601 aaaagacgtc aggccaaact aatacgctca ttcaacaagg tggtggtctt ggatcatcgg
      661 aatcaagatt tattgcgagg ctatttcggc atagaagccg tagtcatacg aagcgggctg
      721 gacgtaaatc atttcaaacc gcaacgtgcc gaattcaata aagtgccggt tatcctgggt
      781 gccggaattc ttttcaggtg gagaaggttt gaagatctca ttagcgccgc agctattgtg
      841 cgtgacgctg gcaagcgttt caagcttcgg attattggca gtgaggatta cgacccgctt
      901 tatagctccg agctccacca gctcgttaca gaacttagcc ttcaagatca tgtcgccttt
      961 ttagggcggg tttcagaaga caccctctta agaaattacc agcaaagcga cgtcttcgtg
     1021 tttcccaatc acaatcaaac ctggggattg gcggtcttcg aggctatggc ttcaggaact
     1081 ccggtgattg tatcgcgcac ctccggcgcc cacgaagtgc taacggataa ggttaatgcc
     1141 ttactagtag atcccttgca tcccgagcaa atcgcccaaa agatcctggg ccttcttgaa
     1201 gatccgaagc tacgcgaaga actggcgcgt tccggccggg agtttgtgga acataacatt
     1261 agctggaagc tctattcaac gaacatgctt aacgcctttc acggaagctc gaacaatgag
     1321 taagcctctg ttcagtatta ttacggtttg ctttaacagc gcccatgaaa tcgagcggac
     1381 gctgaaaagt ctggaaaaac aggtaaatcg agactaccag tacatcgtca tcgacggcaa
     1441 atcaactgat actaccatgg cgatgattga taagtataag catctagtaa ccaagcttgt
     1501 ttcggagccc gacaagggcg tctatgatgc tatgaacaaa gggctcgcac tcgccgaggg
     1561 agagctcatc ttcttcctca acgccggcga tgagctctat gaaaacacta ccctccaaaa
     1621 agtggcggct gactaccaga aggcaggacg ccctgaagtc atatacgggg atattatcga
     1681 gcagccgttg aaaggtgatg cttacgtcag aaaatttccc aaaatatcct atggttatct
     1741 gtatcgggac accatttgcc atcaagcgct attcgctcaa gcgtgcgttt ttgaatacac
     1801 cggtccattc aacctcaaat atagtgtctg tgcggatttc gactggcttc tcaatgccta
     1861 cgctaatcgc agattccgca tccaccaaat cgatgaggtg atgtcaattt tttacctcgg
     1921 gggcatgagc agcaaggtca atggcatggc cgagcgacga agcatcatac taaaacatat
     1981 gccgctcaat gctcgcgtgc ccagggattt ttatgcggcc ggtttcagct taacgaaacg
     2041 catcaagaat aaactccgga ggacgttatg gccaaaaaca ccgtaagcgt ccttgttatt
     2101 acatataatc aaaagggctt tatcgctcaa tgggtcgaga gcctcagggc ccaaaccact
     2161 ccgcctgatg aagtcgtaat cgtagatgat tcttcaactg acggcaccta tgagcaactt
     2221 caagcgatta gcaagaaaca tggctacact ttgatgcgca ccccccggaa ttctggtccg
     2281 tcggcagcca gaaacatcgg tctgcaaagc gtgcatggtg actttatctg ctttctcgat
     2341 ggtgacgatt actttctgcc agagaaaata accgtgcaaa aaaaggcgct ggaaaagaat
     2401 cctaatgctg ttttgtgtta tggtgattgt ttaatcgaca agcaaggaac tatcctgcct
     2461 ttcaacttat cagctcttat gccgctcaac gaaggcgccc tttttgacca gcttctctct
     2521 cgtaatagta tgttgctggg aggggtcatg ctgcgaagcc gcttagctgg aaaccaccga
     2581 ttcgatgaaa ccttaatgcg agccgaggat tatatgtact ttttggaaat ttcaaaaaag
     2641 aatgatacct ttgcgctcgt taaagagccg gtttttgtct accggcgggc caacgcggga
     2701 ctgtcttctc agcgcctcga gaccatagcc tgcaacgaag aggttctccg ccgagtagcc
     2761 ggctgggccc tgcccaaacc acaagtccag cttctgaacg atcaaaaaac cattatttta
     2821 gcagaaaagg ttgatctgct tctgagaagc ggtggggccg gaagtttagc agcccttcga
     2881 gagctggaaa gcctgtcacc ctcccgaagc agacaatggc tgctccggct cttgcattat
     2941 aattccttca ttggccgtat ggcctacgtg ctgtttgaat tttaccgatc gctgcgtcac
     3001 ccgcggaaaa atcaagacga gctcatgatg gagcagttta cccgatgaaa atttttgtag
     3061 aaggcacccc gctatttctt cagcgaaccg gagtcgggca atacactaaa aaccttatgg
     3121 atgttttgtt aaagctagat gaacgcaacc attacaccat attcggtttt ctctttattg
     3181 ggaaacggtt tacaaacaag ccgatcccgg aagggccgaa gctgagctat aagttggttc
     3241 ggtacctgcc ttccaagatt ttcaacgccg tggtgcgcag gctctttctg ccaccgctcg
     3301 atcttttagc cttaagtaaa cccgatatct ttttcttccc caactttgtc cgctatccgc
     3361 tcatcttcgg tgcaaagtcg attgtggtta tatacgacct atcctatatt gttcaggccc
     3421 aattttcggc ttcccctaat cgcaaatatt taacccgcta tgtacctgac tcagtcaaaa
     3481 aagccgacca cgttataaca atttctcaaa actccaaaaa agagattatg gagcactatg
     3541 gtgtgccttc tcagaaaata actattgtta cgccggctat caatcatcag atattctttc
     3601 cgcggccaga taaagaagta gaggcaatca ggcagaaata tgacatatcc aagccctaca
     3661 tcttatacgc gggcacgctc gagccgcgta aaaatatcat tgggatttta gatgcctttg
     3721 ccgagcttga tgccggcttg ctccaaacgc atgccttggt attggcgggc ggcaaagggt
     3781 ggcttgacca tgagatccac gccaaaataa aagagctctc cgagctcgat attattgtta
     3841 ccgggtatct gcctgatgaa gaccttcccg ccatatacag cggcgcttca ctgtttgttt
     3901 atccctcctt ttatgagggg tttggtatac ctccgcttga agctatggcc tgtggagtgc
     3961 cggtgattac ttctgacaat tcgtccttac ctgaggtggt aggcgatgcc ggcatcatga
     4021 ttgacgccca cagcaccccg gcgctgacac gctcaatgca gaaggtgctc accgaccccg
     4081 gcctagtaaa acaattgagg gggcgggggc ttaagcaagc caagcgtttt aactgggaac
     4141 aaagtggtaa gattctactg gaagtaatta atacgattgg gcgcgcataa tggtaatttt
     4201 agtaactggc gcggcgggct ttgtgggccg ccaccttgtg gagctattgc ataagtccgg
     4261 cggcaatgac gtatatggaa ccgctcttca tcatggcttg ggcgacgcta taatgaagca
     4321 agtagatctc aaggacggcg aatcggttaa gcggttgctc tcagagatca aacccgaggt
     4381 catctaccac ctagccgcct tcgcctcccc cgctctgtcc tttaagcagc ccgtcgaagc
     4441 cattactgac accttggcga tgcaaataaa cttgtacgaa gccagcttaa gcctaggcat
     4501 atctccacga tttttggttg taggcagcgg acagatctac ggccgggccg acgccaccat
     4561 gctgccgctc accgaagagg ccgcgctcga ctttgccagt ccctatgcag tcgctaaagt
     4621 tggccaggaa catctggcct ctatgtatgc caagcgtgga attgaatcgg ttatcgcccg
     4681 cccattcaac cacatcgggc ccggccagca acctggctac ctagtttcag acctatcaaa
     4741 gcaagttgcc gagcttgaaa agtcagctag taatgacacc ttgcgggttg gcaatcttag
     4801 ctctaaacgt gattttaccg atgtccgaga tatcgttcgc gcctattgcc tattagctga
     4861 aaaggggagg agagggaagg tctataacgt ctgtagcggc aaatcgacat caggacaaga
     4921 cattctagac acgctcctct ctttctcgga aaaaaagatt gtgaccgagc aggatcccgc
     4981 acgaatgcgt ccatcggaca tccccgacct ctacggcgac gcctcaaagc taaacaagga
     5041 caccggctgg aagccagaga ttgggctttc ccaaaccttg gaagataccc ttaactattg
     5101 gcggtcgcgg gtttaggcct aattggcttt cttgccttcg atttccaggt cgttttttac
     5161 catcatcgta atcagttctt taaagctaac ttcaggttcc cacccgagtt tctgcttagc
     5221 cttggtggcg tcgcctagca gcaaatcaac ctcagccggt cgcataaatc gagggtcttg
     5281 tttaacaaag ggcttccagt catcaatgcc aacctccgca aaggctacat cgagcagctc
     5341 gctaatggcg tgggtttcgt tggtggccag tacataatcg tccggctggt cttgctgcaa
     5401 catcatccac ataccgcgga cgtagtcgcc ggcaaatccc cagtcccgct gtgagtccaa
     5461 attgcctagc accagttctt tttgcaagcc tagctttatg cgcgcaacgg tgctggttac
     5521 tttgcgcgta acaaactcat agccgcgacg aggcgattca tggttaaaca ggatgccagt
     5581 tgaggcatgc atgttatagc tttcccgata attaacggtc atatagtggg catatgcctt
     5641 ggccacgcca taaggactgc gggggtggaa cggcgtaagc tcggtttgcg gcgtttcgcg
     5701 aacctgtcca tagagctcgc tggtggaggc ttggtagaat cggattttgt tgttatcccc
     5761 ccctacccta cggatcgcct cgagcagacg caaggctcca agtccggtaa cgttgccggt
     5821 tatttccggt tgcttgaatg aaaaagccac aaagctaata gcggctagat tataaacctc
     5881 atcaggctgg ctggtctcga ttgcctctat gagggagctc gagtcggtaa gatcgccctc
     5941 caccaactcc accccaggaa tttcctgttc aataattgcc cgcttgggat tgttttgtcc
     6001 gcgcactaat ccgaaaactt tgtaatcttt ttccaacagc agttcggcca ggtacatgcc
     6061 gtcttgcccg gtgatgcccg ttatcagcgc ccgcttttgt gatgccatgc aaaaatccct
     6121 tcttcaatgc attctatagt atcatgggta attcgataca agcagtatag tgtcgtacaa
     6181 taaggaatat gatgaagctt aagcgtttag aacacatact gttatgcggc attttggtaa
     6241 cgcttcccct cgaacggctt ccaagcgtcg atattgctgg ctcaaccgtc cggatcagcc
     6301 aggtcctggg cctgctgtta attgccgttg ttctcccaaa actgtggcga aacaagcgcg
     6361 attggctgaa gccgccgtgg ctgtttttag cactctttaa tctggccgcc tttgcctcgg
     6421 cagcactcag ctcgcgcccc gatcacagca taaaagttgc cggctttatc ttatttgtct
     6481 ccctgctggg ccatacaata agccaggtag ttgaccgtag caggctaaag caatatctga
     6541 cctgggcggg gatcggcgtt gtcggctcgt gcttgtttgg cctctatcag tttattggcg
     6601 acctggccgg actaccctat tggctaaccg gactgcgcga ggaatacgcc aaaggcggca
     6661 tctttgcttt tccgcgaatc cagtcaaccg cccttgagcc cttatacttt tctaactatt
     6721 tgctggtgcc cctggcggtt gccgctacgt atcaggcatt tcgcaaagcc cggaagacct
     6781 ggcccatcag cttcctcatt ctgattgtta ttacactggg gctttcgcgt ggagcccaag
     6841 cggcttccgc ctgtatcatc ctcgtggtag tgggcgttgc cgtctggaag ggtcgtcttc
     6901 gtccggcgct gggcgtactt gccacgaccg tggccgcagt cggggttggc ctgatgctaa
     6961 ttggttttgc cagcattctc tatgagggag gggcgggcaa gcgaaatacc gccagcggca
     7021 atgttaaatc ctttgctaag caggccagca acattaatca aggggaatca gcccaggggc
     7081 gggcccttac ccgccacttg gccgtacggg ctgccatggg gtatccgtgg ctagggcttg
     7141 ggccgggtaa ctttggggtc tacgctatgg aaaagcggcc agacaagttc ggatcagccg
     7201 acaccatcgt caacaatgaa gtattggagg ttgctgctga aaatgggctg atgggcttgg
     7261 tttcgttgct gttgttcgtg ggctttgcgg cccgtgccgc cgttcgagca gccctctctg
     7321 aaaaagacag ctcgatcgct gtgctgatat gggcgcttct gcttggctta ggcggcatcg
     7381 tgttgcaata ccagcttttt tcaacccttt acatcaccca tatttgggtg gccatcgggc
     7441 tgctgctcgg tctttcgcgc ccatcgaggc tacgccggtg aaacgcattg caatcgacgc
     7501 tcggattatt agtacaagca ctggccggta tgtagagcgt ttgcttcatt atctgcaaga
     7561 aagcgaccag cacaaccaat acctggtttt aatgcttact aaagatatgg gctcgtggcg
     7621 cccggtatcg ccgaatttca agattatagc ggcagacttt ccaccctaca gcttcaggga
     7681 gcagtttcag tttacgcttt tgctctaccg gcttcgctta gacttggttc actttacgat
     7741 gccgaaccat cccttgatgt atgttaaaaa acatcttatt actatccatg acttgacctt
     7801 aattaagtac ataaataaac gccgcgaggg ttgggtgaaa aacctttata agcaccttat
     7861 aaagcccttg gttttcaggt ccgtcttgag ggttggggta agtagcgcca gccatattat
     7921 cacgccaacg aagtatgtaa tgaacgctat tcaaaaggac tatggcgttc ccgataagcg
     7981 catttcgtat acgcatgaag ctgccgacgc cctagccagc aaaagccttg agcccaagcc
     8041 gacgatcgca aaacccttta tgctatatgt tggcaatgcc tacccctata aaaatctcaa
     8101 gcgcctggtt aaggcggccg aggaagttaa ccgctcaaag cccatcacct tggtgcttgc
     8161 tggcaagccc gactatttct atgacgaact taaaacctat gtcaccaagg agcacattgg
     8221 caatgtccgg ttcgctggat ttgtctccga cgccgagctg gcctggcttt accagcacgc
     8281 cacactttat gtatttcctt cgctctcaga gggttttggt ctgcccccgc ttgaggctat
     8341 gacctacgga ttaccggttg ctgccgcccg cgctagttgt ttgcccgaag tcctgcagga
     8401 cgctgctgtc tatttcgatc ctcacgatac tagcagcatg gctcaggtta taggagacac
     8461 actacatgat gcggcgaaac tgcggagcct gaaacaagcc ggacttaagc acgttaaagg
     8521 cttttcttgg aggcgcatgg cagaacaaac ccaccggatt tacatccagt ccctcgaggc
     8581 caaatccgac taagccgccc taacgatcag gcggcggcga ttaccttcgc ctcggctctc
     8641 cgacgttacg ccctctacct cggtcagtat cgaatgaatg tggcggcgtt cggcagggct
     8701 catcattggc aaggctacct cttcgccgct agcgcgcacc tgttcgaccg cttcacgggt
     8761 ctgttcctcc agcttttcaa gtcgggcttg gcggtacccg ccaatatcaa cgtggatgta
     8821 tacgcgttcg gtagtttgat gacgaatgag catattcatg atgtgctgca gtgaggccag
     8881 ggtttcgccg cgatggccaa tcaaccggcc tccggcctca gtgtcaaccg aaagcttgat
     8941 ggtgtcgtcg accgactctg cgtggacgtc ggcattaatc tcaaaaaaac tgagcaagtc
     9001 ggcaagatag tgcttggcgc tttccaccag ttcagggctc attcggcgtc tccgctggcg
     9061 gcttctgctt tgggcttggt cttagcttta gcgttggctt tccgtgccgc ggccttgcgc
     9121 tgggccttac ttaaaaccgg cttggctggt gcagcctttt tggcagcaac cacagaattg
     9181 ggatgaatct tgtcttcaac ggcctcgagc tcgtgagcat cccgcaacag cacccaatac
     9241 tgttgaaagg cagcaaccag gctggtaaac atccagtaga gggccagcgc cgaaggaagg
     9301 gtcatggcta ttccggcagt aagcaccgga aatatatagg tcatgttttt catcaattta
     9361 gcctggtctc caccggcagc catagtttga ggcaagagtt gacgggcttg gtaaaactgg
     9421 gcgagcccgg ccagaatagc caatcctaag tggggcttgg taaggtcaat aagtccgaac
     9481 agcgcggggt ggaaggccgc cttctgcttt ataacatcgg ccaccgggcc cagattctgc
     9541 acaaaatcat acgacagctt ggcgatttca tccggacgga cggcgtcgcg cagcacaatg
     9601 taaagggcga aaaagattgg cagctgaacc agcaatggca ggattgaggc aaacgggttg
     9661 gtgccttttt ccttgtagag ctccatgagc atctggcttt ccttttggcg gtcgccgcca
     9721 gcagccttac gcaccttggc gacctcgggc tgcagttttt gcaggacccg ctgcgaatgg
     9781 agctggcgtg ttaccagcgg ccaaagcgcc aatcgtacca agactgtcag caaaataacg
     9841 gctacgccga aatcatgccc tggcaccaag gcatagagca caaacaaaac attaaacaat
     9901 ggttcaacaa aaattgtggt aaacatgcgc ttaaaacacc cctatcatct tagcccgccg
     9961 gatggcctca tcaagatgtt tggccagctc gggagccgga gtttcggcta tattactatg
    10021 cactgttatt actatatcgt atcccggtgt tactgtcgcc cagcgttccg ccagcattgc
    10081 accgatgcgg cgcctgattc tattgcgaat caccgcccgt ttagaaactt ttttacttac
    10141 caccaccacc cctcggctgg cgacagcgcc gctgggggcg gcctttagca caagcggccc
    10201 gctcacaccc tggcggcccc gcctaaacac ccgggcaatg tcgctgggtt gcttaagacg
    10261 gttggggctg gccagcataa acgaaattaa acgcttagct gtttgcgccc tttaaggcgg
    10321 cgtcgcttga gtaccagccg gccggcgcgc gtgctcatcc gctgcaggaa cccatggacc
    10381 cgcattcggc gctttttctt tggctggtac gttcgtttcg gcatgggcta tatcatacgg
    10441 taaagctggg ccaaggtcaa tcacgccctc gtccttttct ctgagtacaa aattgttgtg
    10501 cattttcttt tcccccaaaa ttcgagggtt atgcacactt ttattcctta aagcggtgca
    10561 gctgtggaca aagtcggcgt gtaaaacccg ttgtggacaa gctcaattat ggttatgatt
    10621 atatggctat taaaacgaag tggggtgtat ggaatcgcgc aagctgtggc agggattatt
    10681 gggtgagctg gagctcacca tgagcgccgg aaactttaac acctggctgc gcaacacttc
    10741 aattatcgaa cgcgaaaatg gacatgtagt tgtcggtgtt cccaacattt atactaagcg
    10801 ctggctagaa gaaaagtgcc gcgacgagat tactgaaacc cttacccgca tggatgccac
    10861 ggttcgatcg atcgaatacc gcgtggttac tcgtgccgcc accccgccaa ccagagactc
    10921 ctttattagc ccctcgcctg caccgggccg cccagtggtt gctctgggca cccacgcaac
    10981 cggcccagcc acgctggagc ctgcgaccgt gcagtcggat cgccgcaacc ccacgccgcg
    11041 gctgtttaat ccgcgttaca ctttcgacaa ttatgtggtg ggttccagca atgagcttgc
    11101 gtacgccgcc tgccaggctg tggcaaagta tccgggggag aaatacaacc cgctcttcat
    11161 ttacggcggc gtcggccttg gtaaaaccca tctgatgcag gcggtaggcc acgaaattct
    11221 tcgcgccgac ccctccaagc gcgtgcgcta tgttaccagc gagcagttca ccaatgagtt
    11281 cctgcacagc attcaaacta aaaagactcg cggcttcgcc gacctctatc gcaatctcga
    11341 cgtcttgatt gtcgacgaca ttcagtttct tgggaacaaa gagaagaccc aagaagagtt
    11401 ctttcacacc tttaacaccc tgcatcaggc caacaagcag attattatgt ccagcgacaa
    11461 gcccccgaaa gccataccgc acctagaaga ccgtctgcgc agccgttttg aaatgggaat
    11521 ggccgccgac attcagcgac cagatctcga aacccgagcc gccatcgtgc agcgcaaggc
    11581 tttgtcgcag ggaaccagcc tgtcgttcga aattgtagaa tatttggctc accagtttca
    11641 acacaacatt cgcgagcttg agggtgcgct aacccagctc atggcttact gcgaagttcg
    11701 cggcctagca ccaagcattg gggtggcccg ggaactaaac ggatcaagca gcgattcgcg
    11761 acgccgcaag caagtaacgc cacggttaat tattgagaag acagcggctt tttttgacct
    11821 gctccccacc gacataaccg gcaccaagcg cgaccgcgat attgtggtcc cccgccaaat
    11881 tagcatgtat ctcatgcggg aagagcttga gttgagcttt cccaaaatag cctccaccgt
    11941 cggcggccgt gaccacacca ccgccatgca ttccgttacc aaaatcgagc ggttgatcga
    12001 agttgatgct cgcctgcgta atgagtttaa ccaaattaag gaacagatcg gacgctaagc
    12061 gccctgggta aatccccaaa aatctgtgga cattgacgtc gtttttttga gttctgcaca
    12121 tagacttagt ggttatgctc aaccattgct attttaaccc ccggcccctc cacggcttgg
    12181 ccacaaccat aagcgtgttt acccacagcc tccaagccgc ttttgcttgg gtgtgcgggc
    12241 aaaaaccagt ggttataccc atcttccact gcccctaaga ctatgccttg tttaaattaa
    12301 tgatttttca ggtatactta ggaacaacaa aaggatgtgg aaagaatgaa actcagcgtt
    12361 acccaagaaa actttcataa agccttggga actgttggac gagtggtgag tagtcgcagc
    12421 agccttccgg ttttaagcaa catactatta agcaccgaca ctaaccgttt gcggttgtcc
    12481 gcaaccaact tagaaatagg ggtcacctgt tggattggct gcaaagttac tcaagaaggt
    12541 gcggttacct ccccggctcg gttgctggga gagttcgtat caaatcttcc gagcggaaag
    12601 ctggaactga gcgccagtga ggccaacctg accataacag ccccccacta cgaatcgcac
    12661 attaacggca tttcggcaga cgaatttccg ctcattccag aggtgaaggc cgatccggtg
    12721 gtaacgttgc ccgcagcact ttttcgggaa gccattgccg aggtggtgat tgcggcctcg
    12781 cctgatgaaa accgaccggt cttagccggc gtttatatgt atattgaaga agataacctt
    12841 attttagtag ccaccgacag ctatcgtttg gctgaacgcc acatacccct gggagacatc
    12901 caaacgagca gtctcgacgc tatagtgccg gcacgcacca tgcaggaact agcccgtatt
    12961 ttagccgaca acgatggtga tgtgcgaatg tatttggccg acaaccaagt tatgtttcag
    13021 ctcgacagca ttgagctggt ttcacgattg attgaggggc aattccccaa ctatcgccaa
    13081 attattcccc aggaagaaaa gacggttgct attcttgata cggcagagct tgcccggctc
    13141 actaaaatgg ccagcttgtt tgctcgcgaa aatgccggaa gcattcggat tgaagtgcag
    13201 gctgaaggag agatccgtat tatgtcctca gcgtcccagg ttggcgaaaa tacttcgagc
    13261 gccgaatgcg aggttgccgg cgacgacggt gaaatatcgc tcaacgctcg gtaccttagc
    13321 gatgcgctgg cagccatcaa aaccaagcaa atttcctttg ccatgagcgg caagctgaat
    13381 ccttgtgcac tgaggccaag aagcgaaaac gaagaggaag accaagacga ctacattcat
    13441 attattatgc cgctacgaac ctaaccggcc ccgccgaagg ctatactaga gggcatgagc
    13501 cttacccagc tatctcttac taattttcgc tcgtatggag aacgaacggt tccgcttgat
    13561 gcctcggtaa cgttggtggt ggggcccaac gctaccggca aaaccaacct gctcgaaagc
    13621 ttgtttgtgc ttgccaccac caagtcattc cgcgccaaag acaccgagct gattggccac
    13681 ggccaggaat tttatcggat tgttgcgaag caagacgaca acaccgaact agccatggcg
    13741 tatcaggtaa aatccggaag cgctgaaaaa agagtgcatc acggcaaaac cccgaaaagc
    13801 ctgatcgacc acctggggac aatccgggtc gtgctgtttg agcccaacga tagcctgctc
    13861 atctatggag gtccggatcg gcggcggcgc tatctagact tcgttttaac ccaaaccgac
    13921 aaacattacg ccaagtcgct taatctatat cgacgcacac tacaacaacg gaaccacctc
    13981 ttagcggatt ggggtggaaa taccgccgaa ctgtttgcct ggaacatcca gctggcccag
    14041 gcggctgccg agattgatga gcgccgccgg gccgtagtcg gccacattaa tggcctggcg
    14101 gttgagtcct accgcatcat tgcgggaagc gcagaaccgc tcgagttaac ctatcacggt
    14161 acggcagcag ccgctgacta cgcggcttcg ttcttgaagc ttctcgaggc tgggctgagc
    14221 cgcgacattg ccgctggctt taccaccatt gggccccatc gcgacgactt cagcgccaac
    14281 tttaaggacg ccccgattgc ctcagtggcc tcgcgcggag agatccgtac agtggtgctg
    14341 gcactgaaga tggccgagct ggcatacatc caagaccata ctggctccaa accgctcctg
    14401 ctgctcgacg acgtcttcag tgagcttgat gactcccgcc ggcagcacct cttagacacc
    14461 cttggcaact atcaggcggt agttactaca accaacgccg acatttccag cgaactcggc
    14521 gctcggcatg ccattatata tacaacggag ccccaccatg gatagcatga aagattcttt
    14581 gcgtaaacgg gccgacgagc ttcgactagg cagggccgac gagcttgccg aaatccaagc
    14641 ctacctcgat gaacgttacg caggccagtg ccgtgctgcc agtttaaacg atggggtttt
    14701 aaagataact acgcccaacg ctagcctagc cagcgaacta cgcatggggc aggtagcgat
    14761 tgctcaaaaa tttaaggtgc gcataataat tacgattatg tagaccggat ggctagtatt
    14821 gaaccggctc gattgacacg taggagtggc ttggagcggc taactttatt tgattgtaga
    14881 ttgatcgatc gatcgatatg tcaaaaggcg cctgtccgtc cgcaacgatt gtaagtttta
    14941 atacaccgtt gttttcaacg accacatcgc cttgaacctc aaacttcttt acgtccgaca
    15001 gcttggtgtc tcgcagtttc tccaaggccg cctttacggc tacatattgc tcgggaagta
    15061 caacatcggc tattttttct tctccaacga ccaaaagccc cagctccccc gtcggatttc
    15121 cacgttcctc ctcggtgtta gggttaaagc tgtttggggc gtaatcttcc tggcgtggat
    15181 aaaatacaat aatgagcaca accactaccg caacaatagc cgccccaatg cccacgataa
    15241 gtaaaagtct cttattcatt tctcaaatgc ccataaatgc ttaagcgctc cttggccttg
    15301 caggaaggat cggctaaaag cgcgggtctc gctgtcgccg tgtatgccct tgccgttagg
    15361 gtctgccaaa tagaaggagt ctccgctttc actaaccttt cgaagaacca taaagtggcc
    15421 cgctcccgtg aaatagcctg gatcaaccga aattaacacc agcccgccgt ttctaatggt
    15481 cttttctgcc tcgtcaagat tcaagccgat gtctctcatt gttaaattat agtcctctgc
    15541 aaacactggg aacaatgacc acgcggttcc aaaagtgtga tactcgtccc catatttatt
    15601 tgctaaggtc agcggcgttt cggttccacc cttcagggtt cgcacgatca ttgccaaaga
    15661 ggtaatgcca catcccgatg cgccaattga agactttcct gtcccatacg gcttgctagc
    15721 ccaccttggg tcggtttggt caaaattaac catcttattt gggcccgtca ggtcatatcc
    15781 gaacgccccg gcggttccgc cagcgcccgc tgactcctcg ccgtctacgc agtcaattgc
    15841 cgactcggcg tcgacggcca gctttttctt aagataattt aagatgtcct cagtggtttt
    15901 ctttatctgg ggaccggcat cggctttatt aatgtagacc gtaataacta cttttccggc
    15961 aggagttggg aacacgccgc tattggcggt tacggggttg ccggtagacg ctccgctagc
    16021 caacccccac ttgtttgcct cggagttcaa cccccaggtt tgcgtcgagt ctcgaagagc
    16081 agcttgggcc acttcatatc ctgcccgatc gttggtgtag agcttgtgca ttgcccgggc
    16141 gatgttgcta gccgttgtga cattggcagg cccggagggc gcatctttaa aataacgatt
    16201 aatgtcagta ttggtatagc caagcgagtg cgccattgcc gtaattgccg caaatccccc
    16261 cgcctgccta ataagcatgt tggcgtgtcc gttggaagac ctagcgaggg ctgccgtgat
    16321 cgcatctctt acggtgacaa cgtcccccac cgccagccga tcaccatttg cccccccata
    16381 aagcacagcc gaggttgccg ttatctcgtc gtcgaggttt acgccgctgg tattgagaac
    16441 agcatcaaca atgatcagct taagcatgct tgctggcgtg ttgggcggat cgccctcgtt
    16501 gtatgcctct aaaacatctt gccccaggta ctgtacgaca accgaatgta catcattgtc
    16561 gcgcgcaatg cctctaaggc tttgcgaccc ggcggtttcg gcctggacgg tagtttgaga
    16621 gcctcctaag attttttgca tcgtggagtt cacaaactgt atgtagtcac tggttttgtt
    16681 ttctacggga ggagcgtagc ggtttaaaat ttgttttacc gtcgtaaagt tcgagggctt
    16741 cccaaggtaa agttcgccgc tgataagctg ataatatttt tcaatcgaat ccttaacgct
    16801 ggtgtaactg ccaaatgagc cggctgcgcc attgcgaatg ttgaaaatgt tgttctgcgg
    16861 cgccggccca aatcccgcgg tgcccatacc ggtttctttt tgggcgaacg ccactgccat
    16921 ggctggattc acattatact tttccccaag caaaaccaga ttttcggtat tctccaataa
    16981 tggagagttg gggaagcttt tctggatata gctttttatt ttggcttgca tgtcgcctat
    17041 atcggaaacc ttcgggaaga agggccctaa aagggggcct tgtccggccg cgccctcaac
    17101 cttttctcca cacaccacag cgccgtcgta aaaaggagtg tctggcccgc tctttgcttg
    17161 caggggcgat gaagaaagaa gcagcgctat gacaaggcta aattggatga gtgatttcat
    17221 gggaatgttc ctacgtgtat gtggttaccg tgtgcggcca tggtgtctga gccgtagtat
    17281 cctacacaat taactgtttt gccaccacca acacactgtg tgccaggggc tggcgggttg
    17341 aaaattaatt cgtcaatttt aaactttgta cgattcgcat acagaaactt gtaaagctcg
    17401 tttccttcgg cactgccggc gaagtagcca atatcaattg ccaaccccct ttcatgaagg
    17461 gtgcccggat cgtggtcgca cttgaaagcg gttataggaa ggttgatttg agcattttca
    17521 accacagcaa ccataagtct tataagggtt tctttggcgg ctccgctggt aatgtcggtt
    17581 ttttgacatg gtcgtagcag ctttaggcgg tcattagcca gcagtctttc gcgtagctct
    17641 gcgtctgccc cggcaacgtc accaccggca tcggcagctt cggtttcggg gtcgttgact
    17701 gcctcagggg ggttgaggcg gctctcacac tcttggggca tctcgatagt cgtcccgtcc
    17761 cgtcccttct tt
//
LOCUS       DASVGB010000137         5148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1237101, whole genome shotgun sequence.
ACCESSION   DASVGB010000137 DASVGB010000000
VERSION     DASVGB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237101"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VF272_04300"
     CDS             <1..841
                     /locus_tag="VF272_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7260123.1"
                     /translation="VALSFVQSPSDISSLRQRLHDMKADCGILAKIETKAAAEQVEGI
                     VEAAEGAIVARGDLAVETLPEQVPVIQKRVIESARQHQKIAIVATQMLESMIHSPQPT
                     RAEVQDVATAVAQGADAVMLSGESAVGEYPVEAIALMRRIIVYTENHRWQNITAKAFG
                     EGTQANAISAAAITLAHKMKAKFILAETVSGRTVRNLCSFRPEEMVIAVTPKQRVYQR
                     LALVWGARCYLISDPATAAEESMSLLREEHNVVKGDIVVRASGQRPGVEGGTDTIQVE
                     VVS"
     gene            906..2120
                     /locus_tag="VF272_04305"
     CDS             906..2120
                     /locus_tag="VF272_04305"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7260124.1"
                     /translation="MKFRTLRDLNLKNKKVLLRVDYNVPVSSGIVGDPLRIEASFDTI
                     RYLLNRNCSIILISHLGRPEGKKVPALSLEPVAKKASMMLHHPITFIPECVGPKAEAA
                     AADLKPGEIILLENTRFHKAEDDNDPVFAKQLASLAEVYVDDAFANIHRKHASIVGVT
                     HFLDSAAGLLVEKEVKYVSGALLHPAKPRVAVVGGAKVSTKIGVLDNLIKHVDRLAIG
                     GAMANTFLAEAGHEVGKSKQEPDLFGEVRRIRKDAKAFGVEIVLPEEGVVVSKSLEHG
                     PGRFVHLNEVAKDDYIVDFGPKTVADIVHPLNFHGSIIWNGPLGITEVPEFAHGSRLL
                     ADNIIESGVACIIGGGETAAFVNEQGLHDEFSWVSTGGGASLALMSGSELPGLTALQK
                     EGSKILASLGRS"
     gene            2157..2927
                     /gene="tpiA"
                     /locus_tag="VF272_04310"
     CDS             2157..2927
                     /gene="tpiA"
                     /locus_tag="VF272_04310"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7260125.1"
                     /translation="MKHFTIVGNWKMHQAPDQAVRLVERLQEKATPQTHITNVLCPPF
                     ISIPAVQKVVQNDLFKLGAQNLNEHDEGAYTGEVSGAMLKGMVEYVIVGHSERRRYEH
                     ETDKRIAKKVAAALRNDVTPILCVGEKLSDKQEGHARRVVVDQLQGGLSQITSEEIGM
                     LIVAYEPVWAIGTGEFAEPGQVEPMVKVIRQTIEEMFGESASGSLEILYGGSVSPDNV
                     KAYLKVEHVNGLLVGGASLNYEQFTAIMATAAELSYGR"
     gene            2917..4299
                     /locus_tag="VF272_04315"
     CDS             2917..4299
                     /locus_tag="VF272_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7260126.1"
                     /translation="MAAKKLYFLGITGHTMRGLALAVQQLGYTVIGSDPGSYSVPGED
                     ALDTAGIQWSKLADPAELKGIDGLIISGGTPADDPMLAAAKQRKIKVRSYAEFVGELA
                     KSKRRIVVAGTHGKTTTTSLIAWLLESAGCQPDFLVGVRPHNFGSSVRLGDGRMMVLE
                     GDEYRASTLDDHSKFWFYRPDVLVATSLEMDHPDMFTNVAAIRERFTALVKAMPKDGL
                     MIYWAGSEELKKVAKTSAAAVQSYGIDGDLHVDHVAFGAGGVSFDLYKEDVYQGHFSA
                     PLYGQHNVDNATAAIAVAAAEGLSVEEIQAGLQSFRGAVRRFDIVSPPQAAIRVVDDY
                     AHHPSEIAATIEAARLHFDGRVLAVVRPHTYSRVKELMEGYREAVQDADMAFVASIEG
                     AREAELETVVSGADIARENGNHVVYEPNRRKLIERVVEAARPGDVVVSMTVGGYDGLA
                     AELATRLNQR"
     gene            complement(4286..>5148)
                     /gene="murB"
                     /locus_tag="VF272_04320"
     CDS             complement(4286..>5148)
                     /gene="murB"
                     /locus_tag="VF272_04320"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX7260127.1"
                     /translation="IGQPKLHCVVLKIEIPGRTITAQTDTYTDITVGAGENWDELVAW
                     SVEQNLHGIEALSLIPGTVGAAPVQNIGAYGQELHETLISVEAYDSESAAMVSLSNED
                     CQFGYRDSLFKHEGRGRYVITSVSLRLTPASGSQPANYPRLQVRLIADGITHPTVADI
                     RRTVIAIRQSRLPDPSLIPTAGSFFSNPRVTPEIAETLHAAHPEMPQWPMPDGTVKLA
                     AAWLVEKCGFKGVVENGVGMYDKQAITLINPGRKGPSEIFAFRDRVVAEVEQKFGITL
                     TQEPELVTFG"
BASE COUNT         1183 a         1197 c         1553 g         1215 t
ORIGIN      
        1 tgtcgccctg tcgtttgtac agtctcccag tgatattagc agcttgcgcc agcgattaca
       61 cgatatgaaa gctgactgcg gcatcctcgc aaaaattgaa accaaagccg ctgccgagca
      121 ggttgaggga attgtggagg cggctgaagg tgcgattgtg gcacgaggtg atctggcggt
      181 agaaacctta cccgagcaag tgccggtaat tcaaaagcgg gtgattgaaa gtgcccgcca
      241 gcaccaaaag atcgcgattg tggccacgca aatgttggag tcgatgattc attcgccaca
      301 gccaacccgg gccgaggtac aagatgtcgc cacggctgta gctcagggag ccgatgcggt
      361 aatgctttcc ggtgaatcgg cggtagggga gtatccggtg gaagcgatag ccttaatgcg
      421 tcggattatc gtgtataccg aaaaccatcg ttggcaaaat attacggcca aagcgttcgg
      481 cgagggtact caagctaacg ccatttcggc cgcggccatt acgttggccc ataagatgaa
      541 ggccaaattt attttggccg aaacggtttc cggccggacc gtgcgcaatt tatgttcgtt
      601 ccgtccagaa gaaatggtaa tcgctgttac ccccaagcag cgggtttatc agcggctggc
      661 attagtttgg ggagcccgtt gttatttaat tagtgaccca gccacggcgg cagaggagag
      721 catgagcttg ctgcgcgaag agcataatgt ggtaaaaggg gatattgtag tacgggcatc
      781 cggccagcgt ccgggagtgg aaggcggcac cgatacgatt caagtggagg tagtttcatg
      841 agctttcctg gcccatgtac acattgtgct tggcgcgagc ataagctttt tgctacactt
      901 gcggtatgaa gtttagaacc ctccgcgacc tcaacctcaa aaataagaag gtgctgctga
      961 gggtcgatta taatgtaccg gtttctagcg gaattgttgg tgatccgctg cggatcgagg
     1021 cttccttcga caccattcgg tatttactta atcgcaactg cagcattatt ttaatcagcc
     1081 atcttggccg tcccgagggt aaaaaggtgc cggcattaag tttggagccg gttgccaaaa
     1141 aggccagcat gatgctgcat catccgatta cgtttattcc ggaatgcgta ggcccgaagg
     1201 ccgaagcggc cgctgccgat ttgaagcccg gtgagataat tttattagaa aatacccgct
     1261 ttcataaagc tgaggatgat aatgatccgg tattcgctaa gcagctggca agtttagcgg
     1321 aggtgtatgt tgatgatgcg tttgccaaca tccaccgcaa gcatgcttcg attgtggggg
     1381 ttactcattt tctcgatagc gcggctgggc tcttggtgga aaaagaggtt aagtatgttt
     1441 ccggagcatt gctgcatccg gctaagccgc gggtggcagt tgtcggtgga gcgaaggttt
     1501 caaccaagat cggcgtgttg gataatttaa ttaagcatgt cgaccgcctg gcgattggcg
     1561 gagcgatggc aaacaccttt ttggccgaag ctgggcatga ggttggtaag agcaagcaag
     1621 agcccgacct gtttggcgag gtccgccgga ttcgtaaaga tgccaaggcc ttcggtgtgg
     1681 aaattgtgct gcccgaagag ggcgtggtgg tatcgaaatc actggagcat ggtcctggcc
     1741 ggtttgtgca tttaaatgaa gtagccaagg acgactacat tgtggacttc ggtccgaaaa
     1801 cggttgccga tattgttcat cctttaaact ttcacggcag cattatctgg aatggtccgc
     1861 ttggtattac cgaggttccg gagtttgctc atggatcgcg gctgctggcc gataatatta
     1921 ttgaatcggg tgtagcgtgc attattggcg gtggagagac cgcggcattt gtaaatgagc
     1981 agggactgca cgatgagttc agttgggttt ccaccggtgg cggggctagt ctagccttaa
     2041 tgtcgggcag cgagctaccg ggtctgacag cactgcaaaa ggaggggtca aaaattctgg
     2101 cctcgctagg acgttcctaa aggctcgtat taccataagc gtgttattat agcggtatga
     2161 aacacttcac cattgtcggt aactggaaga tgcatcaagc gccggaccag gcggtgcggt
     2221 tggtggagag gctgcaagaa aaagccacgc ctcaaaccca tataaccaac gtgctctgcc
     2281 cgccgtttat atcgataccg gcggttcaga aggttgttca gaacgatctc tttaaattag
     2341 gcgcccaaaa tctcaatgag catgatgaag gcgcctatac cggggaggta tccggagcga
     2401 tgctgaaagg gatggttgag tatgtgattg tcggccattc cgaacgccgc cgctatgagc
     2461 atgagaccga caagcgcatt gctaaaaaag tagcggccgc cttgcgtaac gacgtaaccc
     2521 cgattctatg cgtgggcgaa aagttaagcg acaagcaaga aggccatgcc cgccgggtgg
     2581 tcgtcgacca gcttcagggg ggcttgagcc agattaccag cgaagaaatc ggcatgctga
     2641 ttgtggccta tgagccggta tgggcgattg gcaccggcga gtttgccgag ccgggccagg
     2701 tagagccgat ggttaaggta attcgccaga cgattgaaga aatgtttggc gaatcggcca
     2761 gcggttcttt ggaaatttta tatggcggca gcgtcagtcc cgataatgtg aaggcttatt
     2821 taaaagtaga gcacgtcaac ggattattgg ttgggggtgc cagccttaat tacgagcagt
     2881 tcacggcgat catggcgacg gcagccgaac tctcctatgg ccgctaaaaa actttacttt
     2941 ctcggtataa ccggccacac catgcgcgga ttagcattag cagtccaaca gctcggctat
     3001 acggttatcg ggagcgaccc aggctcctat tcggttcccg gcgaggatgc tttagatacg
     3061 gccggtattc agtggagcaa gctcgccgat ccggcagaat tgaaaggcat tgatggtttg
     3121 attattagcg gcggaactcc ggcagatgat ccaatgctgg cggcagccaa gcagcgcaag
     3181 attaaggtgc gctcttatgc ggaatttgtc ggggagttgg caaaaagtaa gcggcgaatt
     3241 gtggtggccg gcacccatgg caaaaccacc accacctctc tgattgcctg gttgctcgag
     3301 tcggctggat gccaaccgga tttcttggtg ggcgtgcgtc ctcataattt tggttcgagc
     3361 gtgcgcttag gcgatgggcg gatgatggtg ctggaaggcg atgagtaccg ggcttcaacc
     3421 ctggatgatc attccaagtt ttggttttac cgcccggatg tattggttgc aacctcgctg
     3481 gagatggatc atcccgatat gtttactaat gtagcagcaa tccgcgaacg ttttaccgcc
     3541 ttggtaaaag ctatgcctaa ggacgggcta atgatttact gggctggctc cgaggagctt
     3601 aaaaaggttg caaaaacgtc ggcggcagcg gtgcagtcgt acggcatcga cggcgacttg
     3661 cacgtggatc atgtggcgtt cggggccggg ggtgtgtcgt ttgacctcta taaggaggat
     3721 gtctaccaag gtcatttttc agccccgctg tatggccagc ataatgtgga caatgctacg
     3781 gctgcgattg cggtagcagc ggccgaaggg ttgagcgtgg aggagatcca ggcgggattg
     3841 cagagctttc gcggtgcggt tcggcgtttt gatattgttt cgccccccca agcagcgatt
     3901 cgagtggttg atgattatgc ccatcacccc agcgaaattg ccgcgaccat tgaagcggct
     3961 cgcttacatt ttgacgggcg ggtcctggcg gttgttaggc cgcacaccta ttcccgcgta
     4021 aaagaactta tggaaggtta tcgcgaggcg gtgcaggacg ccgatatggc atttgtggct
     4081 tctattgagg gtgcccgtga ggccgagctc gaaaccgttg tttccggggc agacatagct
     4141 cgtgaaaatg gtaatcatgt ggtgtatgaa ccgaaccggc ggaagttgat agagcgggta
     4201 gttgaggcgg ctagacccgg tgatgtggta gtatcgatga cagtgggcgg ttacgacggc
     4261 ttggcggcag aattagccac ccggctcaac caaaggtaac tagctccggt tcttgggtga
     4321 gcgtaatgcc aaacttctgc tcgacctcag cgaccacacg gtcgcggaag gcaaagatct
     4381 cgcttggacc tttgcggccg ggattaatca aggtaatggc ttgtttgtcg tacatgccga
     4441 ctccattctc taccacgccc ttaaacccac atttttctac cagccaggca gccgctagtt
     4501 taaccgtgcc atcaggcatt ggccactgtg gcatttcggg gtgggcggca tgcagggttt
     4561 cggcgatctc gggagtaacc ctaggattac tgaagaatga gccggcggta ggaataagcg
     4621 aagggtctgg cagccgggat tgtcgaatag cgatcacggt cctacggatg tcggcgaccg
     4681 taggatgggt gatgccatcg gcaatcagcc taacttgaag ccggggatag ttggctggtt
     4741 gggagccgga ggctggtgtt aggcgcaaag acacgctggt aatgacgtaa cgaccgcgtc
     4801 cctcatgctt aaacaaactg tcgcgataac caaactggca atcctcgttc gataagctga
     4861 ccattgcagc tgattcacta tcatacgcct caacgctgat taatgtttca tgcagttctt
     4921 gtccgtaggc gccaatgttt tgcaccggag cggccccaac ggtaccggga atgagcgaca
     4981 gggcctcaat gccatgaagg ttttgttcta ccgaccaggc taccagttca tcccagtttt
     5041 cgccggcacc aacagtaata tcggtatatg tgtcagtctg ggcggtgatg gtgcggccgg
     5101 gaatttcaat tttgagaact acgcagtgca gctttggctg gccgatca
//
LOCUS       DASVGB010000138         6028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_993714, whole genome shotgun sequence.
ACCESSION   DASVGB010000138 DASVGB010000000
VERSION     DASVGB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993714"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VF272_04325"
     CDS             complement(<1..1300)
                     /locus_tag="VF272_04325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7260128.1"
                     /translation="MHKSIKILSYSIFAICGGLLAFFGLPQTGWKALSVATGSMSPAI
                     PPGSLVLIQNVPTTQLHKGDIVAFTNPANPNQTITHRIVKQHNLGTIPGFLTRGDANK
                     SADRPIVGGNIVGKVMWHAPQLGKVFDWSHTWPAIIVLVILPGFIVIIDEYRRMVRVL
                     NVVPSHIKNLLIPLPAGPLFPVVAVASGHQPKRHRSLDGLRPLVLIMVVTVLSTHTAL
                     ALLSTTATLTNNTIATAAKTNHIVISQVNPAVAANVCGVGSTSVSISHTGLGSVNSVN
                     ISSSCVITQNSNTSVSVSNSSNQTATTGSVAANNSTTSGSATSGSVSNTNSTSTTINV
                     TNATALPQVQSFTLHNPTAGAQALAGWTVTDNSAIAKTVPTITIPAGGFATLSLAVGD
                     GLTGSGDRLILKNNLAQTIDALSWGSDVTILDPAVAAIPGG"
     gene            complement(1322..1897)
                     /locus_tag="VF272_04330"
     CDS             complement(1322..1897)
                     /locus_tag="VF272_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260129.1"
                     /translation="MSRSTLAIARATGVIGATVALIVGVTFAQLNTTATLSNNTVSSA
                     TADLTLWDGSAFAQTAPGFTVTNLIPGAGSGPLPFYFKNGGAVDLIVTAHVPVAPSAS
                     GFSGWENLKVTFTDNNGAVQATNMGALLAGEVALPGNPVTAGAQGNAGVPNTEGNYTA
                     TFDITPAAVTGSSATVDSFDIVFTGTAPTPL"
     gene            2065..2553
                     /locus_tag="VF272_04335"
     CDS             2065..2553
                     /locus_tag="VF272_04335"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7260130.1"
                     /translation="MSKNGDHTTRAKQILAHIQYVTLATVTENGEPWNSPVARTLDAE
                     FNVYWISDKESQHSRNVRNNGNAFIVVYDSTVTKSENKAGVYLQAQARELEDSEEIQT
                     ARELIQGPDSKSTEVFSGDGVRRIYQARPQQVWMNSVIMKDGKFVKDIRVELSLGELR
                     AG"
     gene            2740..2961
                     /locus_tag="VF272_04340"
     CDS             2740..2961
                     /locus_tag="VF272_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260131.1"
                     /translation="MSTPGLGGGGSSVNALSSTAGVGGGVAAAVVLPQTGILPVTGTN
                     ISLLLFVSICLAVAIAVSMVAARLSKRAF"
     gene            2996..4267
                     /locus_tag="VF272_04345"
     CDS             2996..4267
                     /locus_tag="VF272_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7260132.1"
                     /translation="MIIIVLAWIITILGIINLSRITLYMIGADWHDVNHHRRKRVIKS
                     RPYRPYISIIVPAHNEESVILRNLSSIYANDYARKEVIVIDDGSSDKTLRRARYFKEK
                     HRLANMHVVHQENRGKAGAINNGIMRAKGSLVMVLDADSALHASALGEVGGYFKDKRV
                     QLAAANIKIIEDGSIFNMMQKFEYLLCYRMKRAQTAFNMEYIIGGIASTFRKSAAKSV
                     NFFDTDTVTEDIDFTLKLIRNDNISKRVIYAPSVIAYTESVQTMSQLIRQRFRWKYGR
                     TQTFIKNKHMFLSRSRKHDWRLTWFQMPFALFSEFTFLLEPLLIGFILYIVIRYQDFG
                     TLLGAYAVMTIYLALNVIAEETETRRSKLRLTALAPLQYPFTFVLTAVEYAASIKTLI
                     HMRQLVRGGGESRWNHVERSGAAVEAVHRAV"
     gene            4326..4955
                     /locus_tag="VF272_04350"
     CDS             4326..4955
                     /locus_tag="VF272_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260133.1"
                     /translation="MSRVLLKRSLLVGATSLMIAGSAFAIGSSTGSDSGSQTTRKGDQ
                     NQRTALTQTGDDRRGEVKTRLEASNLSRCEKREAKINAILTRAATRGERQIAVFHKIS
                     ERTQTFYTTKGRTVSNYDALVFEVNSKKTAAQAAVEAVKATSVSFTCDGTDPKGAAKQ
                     FKDNLKAETAALKAYKTAVKNLIVGVKSAQGTSSSTENKTKRKTEGEQQ"
     gene            4952..5248
                     /locus_tag="VF272_04355"
     CDS             4952..5248
                     /locus_tag="VF272_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260134.1"
                     /translation="MKLLKNQSGFAILELALIVAVLAVVGYVAYDAYNAKQTATKTEA
                     PATVKQSNGVNDVPAAPEVNSTEDLTAAEKTLDQIDPEANATDSAQLDSQLSAF"
     gene            complement(5252..5596)
                     /locus_tag="VF272_04360"
     CDS             complement(5252..5596)
                     /locus_tag="VF272_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260135.1"
                     /translation="MDSQSERKLAENELIFKKHNERIKRGVEKLVAQSKVDDLTLNLF
                     CECSNEKCQKMVAVSTSDYDRVHRNNRYFIVLRGHENMQVEKVIEKNSHFSVVEKLIT
                     PPATTGMQLNPT"
     gene            5692..>6028
                     /locus_tag="VF272_04365"
     CDS             5692..>6028
                     /locus_tag="VF272_04365"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008106875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7260136.1"
                     /translation="MTTAFQFSLQNQLGRTYTLQSFAGRWLVLYFYPKDDTPGCTAEA
                     CSIRDSYQDLLNLGADVVGISKDTTKSHEKFAQKHGLQFSLLSDPTGSTIKAYGAWGK
                     TMVGVKGILR"
BASE COUNT         1609 a         1454 c         1505 g         1460 t
ORIGIN      
        1 gaccaccggg gattgcagcg accgcggggt ccaaaatagt gacatcgcta ccccagctca
       61 gagcatcaat tgtttgggca agattattct taagaattaa ccggtcgcca ctgccggtaa
      121 ggccgtcgcc aaccgccaaa ctaagtgttg caaagccacc ggccggaatg gtaattgttg
      181 gaacggtttt tgcaatggcg ctattgtcgg ttaccgtcca gccagccaaa gcttgagcac
      241 cggcggtagg attgtgcaag gtaaacgact gaacttgcgg taatgctgtt gcgttcgtaa
      301 cgttgattgt cgtgctggtt gagttggtat tgcttacgga accgcttgtg gcgctaccgc
      361 tagtggtgga attattagca gctacactac cggtagtagc tgtctggttc gaactatttg
      421 aaaccgaaac gctggtgttg ctgttttggg tgatcacaca gctggaactg atattcacgc
      481 tatttacact gcccagaccg gtatgggaaa ttgataccga cgtacttccg accccgcaga
      541 cgttcgcagc cacagcagga ttcacctgag atatcacaat atggttggtt ttagcagccg
      601 ttgctatcgt attgttggtt aaggtagcgg tggtcgaaag cagtgcaaga gcggtatggg
      661 tgctcaatac cgtaacaacc ataatgagca caagcggcct caagccatcc agtgagcgat
      721 ggcgtttggg ttgatgcccc gacgctaccg cgacaaccgg aaacaacggc ccggccggta
      781 aggggattaa caggttttta atatgagaag gcaccacatt caatacgcga accatcctcc
      841 ggtactcatc aataatcacg atgaagcctg gaagtattac gagcacaata atagccggcc
      901 aggtatgcga ccagtcgaaa accttaccaa gctggggggc atgccacata accttaccaa
      961 caatgttgcc gccgacgatt ggcctgtcag ccgacttgtt agcgtcaccc ctggtaagaa
     1021 agccaggaat ggtaccaaga ttgtgctgct ttacaatccg gtgcgtaatg gtctggtttg
     1081 ggttggcagg gttggtaaac gctacaatgt cgcctttatg caactgagtt gttgggacgt
     1141 tctgaataag cacgagcgaa cccggcggga tcgcggggga catactgccc gtggctacac
     1201 tcaaggcttt ccagccagtt tgaggtaagc cgaagaaggc gagtaacccc ccgcagatcg
     1261 caaatatgct ataggataga attttgatac ttttgtgcat attccgctcg gttcattttg
     1321 gttagagagg cgttggcgca gtaccagtaa agacgatgtc gaacgaatcg accgttgcac
     1381 tactaccggt cactgccgcc ggagtgatgt caaatgtggc agtgtagtta ccctcagtat
     1441 tgggcacacc ggcgttacct tgcgcgccag cagttaccgg gttacccggt agcgctactt
     1501 cgccggctag taaagcaccc atattggtag cctgaaccgc tccattgttg tcggtaaagg
     1561 taactttcaa attctcccat ccactgaacc cacttgcgga aggtgctacg ggaacatgag
     1621 ccgttacgat caagtctacg gcgccgccat ttttaaagta aaaaggcagt ggaccgcttc
     1681 cagctcccgg aattaagttc gtcaccgtaa aacccggggc ggtttgggca aacgccgatc
     1741 catcccacaa cgttaaatct gccgtggcag atgaaaccgt attattactt aatgttgctg
     1801 tcgtgtttaa ctgcgcaaac gtaacgccga ctatcagcgc cacggttgca ccaatcacac
     1861 cggttgcccg agcgatcgcg agagtacttc tactcatatg catgcctcct caatttgtta
     1921 cgtatgtaca agcataggtt gaatcactac ggccgcgctt cggccgcatc gctaatttat
     1981 agcggttgaa tggtcggctt tttgtcatct ttgttcgcta ttacataaat gcagggagct
     2041 aatcaatact gctatagttg gggcatgagt aaaaatggtg accacacaac cagagccaaa
     2101 caaatcctcg cgcacatcca gtacgtaaca cttgcgaccg taactgaaaa cggcgaaccc
     2161 tggaacagcc cggttgcccg aacgctggat gccgagttta atgtttattg gatttcagat
     2221 aaggaaagcc aacattcccg taatgtccgc aacaatggca acgcgtttat agtggtatat
     2281 gattcaaccg ttactaaaag cgaaaacaag gccggcgtat acctacaggc tcaggcgcgg
     2341 gagcttgaag attccgagga aattcagaca gccagagagc tcatacaagg cccggattcg
     2401 aaaagcaccg aagtgtttag cggagacggc gtacggagga tttaccaggc gaggccgcaa
     2461 caagtatgga tgaacagcgt cataatgaaa gacggaaaat ttgttaaaga cattcgcgtt
     2521 gagctgtcac taggcgagct tagagcaggc taatactagc accgagctta tcggccaatg
     2581 cccctcttta ggctgaatga gtatcaaaaa agacaatcgc ttcatgaaat gctttacaaa
     2641 ttaatacttt taggttatta tatggctctt attaggtagt tctaggttgt ggaggtgcac
     2701 aacgagggtc aagaactcgt taagtgtaag gagatttgga tgtctacacc aggattaggc
     2761 ggcggcggca gtagtgtcaa cgcccttagt agtacagcag gagttggcgg cggcgtcgct
     2821 gcagcagtcg tgcttcccca aaccggcata ttaccggtaa ccggaaccaa tattagctta
     2881 ttgctgttcg tcagcatttg cttagctgtt gccatcgcgg taagcatggt tgcggccagg
     2941 ctttctaagc gcgcattcta acctattccc gtcttcgggc gggaagagaa cttatatgat
     3001 tattatagtc ttagcttgga tcataacaat tctaggcata atcaacttaa gccggattac
     3061 cctgtatatg attggcgccg actggcacga tgtgaaccat catcggcgta agcgcgtgat
     3121 taaaagccgg ccctaccggc catatatttc cattattgtg ccggcccata acgaagagtc
     3181 ggtaattctg cggaacttga gctccatata tgccaacgac tatgcccgaa aagaagtaat
     3241 tgtgattgat gatggatcga gcgataaaac gctgcgccga gcccggtact ttaaagaaaa
     3301 gcaccgttta gcgaatatgc atgtggttca ccaggaaaat cggggcaaag ccggggcaat
     3361 taacaatggt attatgcgcg ctaaaggaag ccttgttatg gttcttgatg ctgactcagc
     3421 attgcatgcc agtgcgttag gcgaagttgg cgggtacttt aaagacaagc gagtacagct
     3481 agcagcggca aacattaaaa ttattgaaga cggcagcatc tttaacatga tgcaaaagtt
     3541 tgaatactta ctgtgctacc ggatgaaacg agcgcaaacg gccttcaaca tggagtacat
     3601 tattggcggc attgcttcaa ccttccgtaa gtcggcggca aaatcagtca acttttttga
     3661 taccgacacg gtaaccgaag atattgattt cacccttaaa cttattcgca acgacaacat
     3721 ttcgaaacgg gtgatctatg ccccgagcgt gatcgcctac accgagagtg ttcaaaccat
     3781 gtcccagcta attcgccaac gtttccgctg gaaatacgga cggacgcaaa cctttattaa
     3841 aaacaagcat atgtttctca gccgcagccg taagcatgat tggcgtctaa cttggtttca
     3901 aatgcccttt gcgctgttta gcgagtttac gttcctactt gagcccctct taatcgggtt
     3961 catcctttac attgtgattc gttatcagga ctttggcacg ctgctgggcg cgtacgcggt
     4021 aatgaccatc tacctggcgc tcaatgtgat tgctgaagaa accgaaactc gccgctcaaa
     4081 actgcgtcta acggcgctcg cgccgcttca gtatcccttt accttcgtgc tgacagcggt
     4141 tgaatacgcc gcttcaatca aaaccttaat tcatatgcgc caattagtgc gcggcggcgg
     4201 tgaaagccgt tggaatcatg tggagcgatc aggtgcagcg gttgaagctg tgcaccgcgc
     4261 tgtttaacac tatttgacga atgtccgtaa ggagcataag cttttataca ggaggatatt
     4321 gttcaatgtc tcgtgtatta ctaaaacgaa gcctgttagt tggtgctacc tcgctgatga
     4381 ttgctggcag tgctttcgcc attggatcca gcactgggtc tgattccggc agccaaacca
     4441 cccgtaaggg agatcaaaat cagcgaaccg ccctgacaca gacaggggat gacaggcgcg
     4501 gagaggtaaa aacccggctg gaagccagta atttaagccg ctgcgaaaag cgcgaagcta
     4561 aaattaatgc cattttaacc cgcgcagcaa cccggggtga acgccaaatt gcagtatttc
     4621 ataagatttc cgagcgcacc cagacgtttt acaccaccaa gggtaggacc gtaagtaact
     4681 acgatgcgct ggtgtttgag gtaaattcca agaaaaccgc cgctcaggca gctgtcgaag
     4741 ccgtaaaagc cactagtgtt tcgtttacat gcgacggtac cgacccgaaa ggtgcggcca
     4801 aacagtttaa ggacaacctg aaggccgaaa ccgctgccct gaaggcctat aaaaccgcgg
     4861 ttaagaactt aatcgtcggt gttaaaagcg cgcaaggaac aagcagttcc acggaaaaca
     4921 agacgaaacg taagaccgaa ggggagcagc aatgaaacta ctaaagaatc aatccggctt
     4981 cgcaatcctt gaattagcgc ttattgtggc ggtacttgcg gtcgtcggct acgttgccta
     5041 cgatgcttat aatgccaagc aaactgccac aaagacagag gcgccggcaa ccgtaaaaca
     5101 gtctaatggg gtgaatgacg taccggctgc acctgaggtt aattcaactg aggatttaac
     5161 agccgccgaa aagacgcttg atcaaattga tcccgaggct aacgccaccg acagcgccca
     5221 gctcgatagc caactatcag cgttttagca cctaggtcgg attaagctgc atgccggttg
     5281 ttgcgggagg agtaattaat ttctccacca cgctaaaatg actatttttt tcaataactt
     5341 tttcaacctg catgttttca tggcctctta gaactatgaa ataccggttg ttacgatgta
     5401 cacggtcata atcagaggtg ctaaccgcaa ccatcttctg gcatttttca ttagaacact
     5461 cacagaataa attgagggta agatcatcaa cctttgactg cgcgaccagc ttttcaacgc
     5521 cacgcttaat ccgctcgttg tgctttttaa aaataagctc gttttcggcc aacttgcgct
     5581 ctgattgtga gtccattatc tagctccact tcgtccatta agatataata gtatctcact
     5641 gggaacaaat gtacaggttt tagaagccgc gcttgctata atcatcttta catgaccacc
     5701 gcttttcaat tttcattgca aaaccagctt ggcagaacct acacgctgca atcatttgcc
     5761 ggtagatggc tggtattata tttctatccc aaggatgata cgccgggttg caccgcagag
     5821 gcctgcagca tacgggattc gtaccaggat cttctcaacc ttggcgccga tgtcgtcggc
     5881 attagcaaag ataccaccaa gagccatgag aagtttgccc aaaagcatgg attacagttt
     5941 tcactattga gcgaccccac cggatcaacc attaaagcgt atggggcctg gggtaaaacc
     6001 atggtgggcg tgaagggaat tttgcgta
//
LOCUS       DASVGB010000139         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_1237142, whole genome shotgun sequence.
ACCESSION   DASVGB010000139 DASVGB010000000
VERSION     DASVGB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237142"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF272_04370"
     CDS             <1..280
                     /locus_tag="VF272_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7260137.1"
                     /translation="SPEFVPLFITIISINIALMVFNLIPLPPLDGSRILYAAFPPIRN
                     LFDRLERTGLLIIFAILLLAGPLFIPFISAITGAILQILVPGLSALST"
     gene            481..556
                     /locus_tag="VF272_04375"
     tRNA            481..556
                     /locus_tag="VF272_04375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:514..516,aa:Pro,seq:ggg)
     gene            648..1541
                     /locus_tag="VF272_04380"
     CDS             648..1541
                     /locus_tag="VF272_04380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7260138.1"
                     /translation="MFKLIVGAMMAAVLLSATTASALASVVPQRLAATASADPGVILS
                     LGDSLAVGLQPAKFGFRPTLGYAGVVAEQMSKLMPGIKLVRYGCAGATTTSFIRGGPC
                     PFSNPPYGRGSQLIAAERFITANRGRVKLVMIDIGANDVVHCARANFGPLKTGCLAVM
                     LPRVRKNVALIAQRLRQAAGPSLRLVGLNYYNPFLAAYVQGGKGRSRAMASVSLIRNK
                     FNDRLVQAYRQHGFVVADVARAFGSDLPFSTMTVLSPYGRIPASVANICKHTWACDRR
                     SDIHARDSGYGLMAAAVLTAT"
     gene            1647..2090
                     /locus_tag="VF272_04385"
     CDS             1647..2090
                     /locus_tag="VF272_04385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX7260139.1"
                     /translation="MSELINDVNRRATLVINTDGGSRGNPGPSAIGVVITTPDGQHLE
                     SFGKYLGEGTNNFAEYSGVIAGLEAAAKYQPKTIQFLLDSELVVKQLNGQYRVKHPDL
                     QPLYHQVKALAEPYDVSFQHVLRGKNHLADTEVNKALDEAAHSAA"
     gene            2110..>3133
                     /locus_tag="VF272_04390"
     CDS             2110..>3133
                     /locus_tag="VF272_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7260140.1"
                     /translation="MKNIFKLYRYIADERNRLIWTAVISALLSLGSTATPLFFKYVID
                     QLVAIANGQASAQATTNITLVIAALAGVLLVSSFFGFIRERLSDRLSADLLVKLNRRL
                     FEHMMTLSIDYYERTKVGETMTKVNNAIFQFIFWLQGLTEGTLSQVMQLILATALLWY
                     ISPLIGPVVLLLIALGVTIQAVRIYQSNHIRRAARMQFELAGGHFNETISHIATIRSS
                     VADKAPLVKFDDYLAEARRLTYLQNNIEQRGNLARDIVNNLAILSGVGIIAWQSLKGR
                     ATPGDVVAVALYLQQITSSIGPLGRLLVTTSQVETSVERIVELLEIQPTVKDAPGAKK
                     LSKLRTL"
BASE COUNT          670 a          849 c          832 g          782 t
ORIGIN      
        1 aagtcccgaa tttgtcccgc ttttcattac gattattagc atcaacatcg ccttaatggt
       61 gttcaacttg attccacttc cgcctctcga tggatcgcgc attttatatg cggcttttcc
      121 gcccatccgc aacttgtttg accgcttaga gcgcaccgga ttattaatca tttttgcgat
      181 tctgctcttg gccggcccgc tgtttattcc gttcattagt gccattaccg gagctatttt
      241 gcagatactg gtaccgggac tttcagcctt atcgacgtga ggcggcaggg caggctatac
      301 tgaatatagc tctgcagcat cggtagtctg gcttattttt tctgtatgat taatctccag
      361 ggaacctaag attagctatt accgaggtga tagtcttgcg ggtacccacc tgcttttggg
      421 caggaaagag atgccaagta ctaccatgct gcggggtaaa gaggattaac acggtgattt
      481 cggggtgtgg cgcagttggt agcgcgtacg gctgggggcc gtgaggtcgc aggttcaagt
      541 cctgtcaccc cgaccagcaa ttatttgaag gatatggtat agtagcacag ccatttttcg
      601 cagtacgaat ctgataagca gtacgaactc ggaagagggg tccttgtatg tttaagctga
      661 tcgtaggtgc catgatggct gcggtgttgc tttcggctac caccgcttcg gcgttggctt
      721 ccgttgtacc gcagcggctt gctgccacag cctcagccga ccccggcgtc attctgtcgc
      781 ttggcgactc gctcgcagtc gggctgcaac cggcaaagtt cgggttcagg cccacgcttg
      841 gctatgccgg ggtagtggcc gagcagatga gcaagctgat gcctggcatc aagctcgttc
      901 gctacggctg tgccggagcc accaccacgt cgttcatccg cggaggcccc tgcccgttct
      961 ccaacccgcc gtacggacgc ggttcgcagc tgatcgccgc cgagcgcttc atcaccgcca
     1021 accgcggtcg cgtcaagctg gtgatgatcg acatcggtgc caacgacgtc gtccactgcg
     1081 ccagggccaa cttcggcccc ctcaagacag gctgtttggc ggtgatgttg cctcgggtga
     1141 ggaaaaacgt cgcgctgatc gctcaacggt tgcgccaggc ggccggtccc agccttcgct
     1201 tggtgggctt gaactactac aacccattcc tggctgccta tgtgcagggt ggcaagggta
     1261 ggtcccgagc aatggcgtcg gtctccctga ttcgcaataa gttcaacgac cggctggttc
     1321 aggcctatcg ccagcatggg ttcgtcgtgg ctgatgttgc gcgggccttc ggttcggacc
     1381 tgcccttcag cacgatgacc gtcctttcac cctacggacg aatcccagcc tccgtcgcca
     1441 acatctgcaa gcacacctgg gcatgcgatc ggcgttcgga catccatgct cgcgacagcg
     1501 ggtacggcct catggctgcg gcggttctga ccgccactta agcccttcta ctcgcagagc
     1561 gaaagccctc cgggtaggag ggctttcgtc ttatttgcaa ggccgctttt tgaccaattc
     1621 tggcaaatat cgtagaatag gtcgatatga gtgaattaat caacgatgtt aaccgccgag
     1681 ccaccttggt aataaatacc gacggaggtt cgcgcggcaa tcctggccca tctgcaattg
     1741 gtgtagttat cacaacacct gatggtcaac acttggaatc atttggcaaa tatctcggcg
     1801 aggggaccaa caactttgcc gagtatagcg gcgtgattgc cggccttgag gctgccgcta
     1861 aatatcaacc caagactatt caatttttgc ttgatagcga actggtagta aagcagttaa
     1921 acggccagta ccgggtgaag catcccgatc tccaaccact gtatcatcag gttaaagcgc
     1981 tagcggagcc ttatgacgtt agcttccagc atgtactgcg gggtaaaaac catttggccg
     2041 ataccgaggt taataaggct ctcgatgaag ccgctcatag cgctgcctaa ccggctctag
     2101 aaaaccatta tgaaaaatat ctttaagctc tatcgctata tcgccgatga gcgcaaccgc
     2161 cttatttgga cggccgtcat cagcgcactt ttaagcctgg gtagcaccgc gaccccgcta
     2221 ttctttaagt atgttattga ccagctggtc gcaatcgcca acggacaggc ttcagcccag
     2281 gcaacgacca atataacgct ggttattgct gccttggcag gagttttgct ggtaagcagc
     2341 tttttcggat tcattcgaga gcgtctttcc gaccgcttaa gcgctgatct gctggtgaag
     2401 cttaatcgcc ggttgtttga gcatatgatg acgctgtcga ttgattatta tgagcgcacc
     2461 aaggtcgggg aaaccatgac taaggttaac aatgctattt tccagtttat cttttggctg
     2521 caggggctca ccgaaggaac attgtcccaa gtgatgcagc tgattttggc aaccgcactg
     2581 ctctggtata tcagccctct gattggtcct gtggtgcttc tgctcattgc tctcggagta
     2641 actatccagg cggtacggat ctatcaatcc aaccatattc gccgcgctgc ccgcatgcag
     2701 tttgaactag ctggtggcca ttttaacgaa accatcagcc acattgccac cattcgcagc
     2761 tcggtggccg acaaggcgcc gctggtaaaa tttgatgact atctagccga agcccgccgc
     2821 ttaacctacc ttcagaataa tatcgagcag cgcggaaatt tggcccgcga cattgtaaac
     2881 aatctcgcga tcttaagcgg agtcggtatt attgcctggc aaagtttgaa agggcgagcg
     2941 actcccggtg atgtggtagc cgttgctctc tatttgcagc aaataacgtc ttcaataggt
     3001 ccgcttgggc gcttgcttgt gactaccagc caggttgaaa ccagcgtgga gcgcatcgtc
     3061 gagctgttag aaatccaacc cactgtcaag gatgccccgg gagctaaaaa gctttcaaag
     3121 ctgcgtacgc ttg
//
LOCUS       DASVGB010000140         9853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_929880, whole genome shotgun sequence.
ACCESSION   DASVGB010000140 DASVGB010000000
VERSION     DASVGB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929880"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..339)
                     /locus_tag="VF272_04395"
     CDS             complement(10..339)
                     /locus_tag="VF272_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7260141.1"
                     /translation="MQFVIIGLDGTDKEAHTRRQAVRHDHIAMGDKLLESGNLWYGAA
                     LLNDDGSTKGSMYVVSFPSEKELQEWLDKEPYIVGGVWRHITIHKSNTRDPWQFNRPQ
                     EWFEKNT"
     gene            complement(531..605)
                     /locus_tag="VF272_04400"
     tRNA            complement(531..605)
                     /locus_tag="VF272_04400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Glu,seq:ctc)
     gene            complement(695..1825)
                     /locus_tag="VF272_04405"
     CDS             complement(695..1825)
                     /locus_tag="VF272_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX7260142.1"
                     /translation="MTTTTKLNYVPGNRVATLLNAVEAYPVMLRAIHNATTSIYIAHF
                     CYQKGKASDLFYQPLLTAARRGVKIYLLIDAYGSNEFDRNQLKKMRLDHINVRWFNPR
                     KTLHPLRYNKRMHKKLLIIDNSIGFSGGIGIADFWLDDPSYPEPWRDTHFQLEGPVIS
                     ELSHSFITSWNRFSSEQLPLQKAEPAPVHGGTIPLALVDTAPIDPANRSNITNVFTRL
                     IGEAKGSIDISTAYFGPTAAVRRSLHAAAKRGVSVRIITNGPHPSHWQAMQAGRHHYN
                     GLLEAGVDIFEYQRTKIHAKITIIDRTLSIIGSSNLNTRSFHHDEEAIMVADDMKLAT
                     GLTKQFEDDLESSKPIELKEWRQRPLTEKLQQAAASIGRYFF"
     gene            2170..2799
                     /locus_tag="VF272_04410"
     CDS             2170..2799
                     /locus_tag="VF272_04410"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEX7260143.1"
                     /translation="MRRFALMLLLIAGTANLNVGKIQATDSKIKPSKIAAVAEAPKEA
                     PQKHVVAAGESLSSIATDKQLESWRPLWNANTELQNPDQINPGQELIIPKDPTADRPL
                     PPGYGEPVAVAAQAPAQQSYRSAPQAKVANPAPSGDILSRIRMRESGGNYATNTGNGY
                     YGAYQYDVGTWNNYGGYRYASDAPPAVQDAKAAETYARRGCSPWPSTCY"
     gene            2836..3336
                     /locus_tag="VF272_04415"
     CDS             2836..3336
                     /locus_tag="VF272_04415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7260144.1"
                     /translation="MKHSTVVGIGGSLVIAVVVVGSTLAFSQTAKKAPGASQDSNKPG
                     IETPPRTKEQTIQGDTMELQKTDEVVGTGPEAVAGKKVTVHYLGTLTNGTKFDSSYDR
                     NEPFGFTLGGGQVIKGWDEGVAGMKVGGKRKLVIPPEMGYGNQAVSTIPANSTLVFEV
                     ELLGVE"
     gene            complement(3353..4525)
                     /locus_tag="VF272_04420"
     CDS             complement(3353..4525)
                     /locus_tag="VF272_04420"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX7260145.1"
                     /translation="MDDREREAQNVPIQISSSQNQMPQPEPPVEPPAKKPESNNRFKA
                     FLATHHTRIIIVAGLLIIAPVSAWAYIQYTKPDTPISSTAKVPKKKPPVPTTKASPLT
                     GVELEPALADRAIRAIVIENHPESRPQSGLSQAGVVYEALAEGGITRFLAFYVEGQPP
                     SIGPVRSLRPYFNDWALEYNSPIAHAGGSAEALSQVGPLNVKSMNALGGGVNQFFTRA
                     SDRYAPHNLYTSSEGMDRLVESRGYAHPAQFTPNPRKKDQPLATPAHGVIGINYSYTG
                     FQVEFRYNKDCNCYDRFMAGAPHIDRNTGAQIQVKNVVAQYMSVRYDATGHAILGILG
                     SNKVLVFRDGGVIEGTWAKASYPERTKLVDTAGKEIPLNKGNTWFSIVPEDRPVSF"
     gene            complement(4547..5941)
                     /gene="gltX"
                     /locus_tag="VF272_04425"
     CDS             complement(4547..5941)
                     /gene="gltX"
                     /locus_tag="VF272_04425"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7260146.1"
                     /translation="MTQPVRVRFAPSPTGYLHVGGARSALFSYLFAKRHKGSFILRIE
                     DTDRQRLVPDAVEQITESLDWLGMQADEGPVFQSERLDLYAEQAQLLLKDGVLYEDYT
                     TPEQLEQMRTAAQKAKQPFRFRYEQAALKPATPNQKPVMRFRVTDGEDVIWQDAVWGE
                     QRWQRGVIEDFVAMKADGYPTYHFANVVDDHLMKISHVIRAAEWIPSTPKHLLLYEAF
                     GWEPPVFAHLPQVLGADKAKLSKRHGAKSVLEYRDAGYLPEALVTYLASLGFNDGTTQ
                     ETYTADELIITFSFDRVQSSPAVFDPERLVWMNGVYIRQLPLDDLYQRTQGFWPAEAK
                     NSKETYRKQVLALVHERLKFFAELPLLTSFFFTEPAIDPNLFPKQLDKDQRTQILGAT
                     LAALENSDFSEADLEQRLRHLVEKQGLKTGVMFGLIRVAITGSTAAPGLFETLHALGR
                     ETSLRRLANAVTLP"
     gene            complement(5978..6319)
                     /locus_tag="VF272_04430"
     CDS             complement(5978..6319)
                     /locus_tag="VF272_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260147.1"
                     /translation="MNDNAWERLVDSIDATLGIERHGREKQPLPDQPSLMQSVEFFEF
                     SRGEEHFRLERSSGPAVVDRKTHYSHREGTANRIEYIYDENETAHKVTLYRRDNPSSN
                     WEAIDLTQLGL"
     gene            complement(6316..7350)
                     /locus_tag="VF272_04435"
     CDS             complement(6316..7350)
                     /locus_tag="VF272_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260148.1"
                     /translation="MQCPKCGKTGFVAINGKRFCSNCGATVVAGTAPSSMSEITSATS
                     TLDLRSDPAVQAAPQAPKPLPAGQLHGRQIAGAALRDLRPAPSVPPNPPSPATASSSY
                     EAVAAPTPTPAPQSPAPAAAQVMPPSPAADTRSPLIQKLPPHPALARPEPNPLPNAVK
                     ANVGSLAATTPAPVPTPESSDLQQALKAAKSSTTVPMVTKVAAAIATIGVMSGLIWLQ
                     NAPKLAFHNAAAKAGIDASLPTYIPSSYRQIGPTNTSNGRISINFTNASSETPLSIVQ
                     RRTNWDSNSLRENYVSRQTANFLAVQGQGLVIYLDDDQANWVNHGIWYQIIGTSKLGR
                     EQVLKIAYGL"
     gene            complement(7387..8265)
                     /locus_tag="VF272_04440"
     CDS             complement(7387..8265)
                     /locus_tag="VF272_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX7260149.1"
                     /translation="MAKTSVPILGVNIDVLTMEQAAGLITRQAAPGKPPAYVVKPYVE
                     FFDRAHRDPDVRKLLNQAWLRLPESVSAQWAAAYLAGPPGLVRVLGLATSIIVRPRAI
                     KRPIAEKFGGSVFTWKLLEACMKQQLRVYLIGSPRGSDISATARTIQTRLPQLDIAGT
                     WPGQWGGMAGEQLRKRLQTTPLEAKLLSDLQERKPDIILVGMGFPLQEELMAKLAPQL
                     AHGVLIGEGGTFDYDSFGGTRTKAPQRIQQIGLEWLWRLLLEPARLHRQLAVPRFMWM
                     VYRESRKQVKTLPTSS"
     gene            complement(8252..9376)
                     /locus_tag="VF272_04445"
     CDS             complement(8252..9376)
                     /locus_tag="VF272_04445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7260150.1"
                     /translation="MKVALVHDWLNTKQGGGEKVLMEMATIFPNAPIYTLLYDGSVFN
                     GRIDSTRIRPSSLQCWPVFLRKRPRYLLPWLPAAIEQFDLSEYDLVISSSSAFAKGVI
                     TKPGTLHICYCHSPMRFVWDYWPAYVHEQRVGPLRRAVMYALTSNVRQWDYMSAARVD
                     RWIANSKTTAGRIAKYYRVKVDAMIYPGADIEHFTPVEHKGSYFVTLSSLTPYKKIDV
                     AIAACNKAGVELVVIGDGADRQRLEQLAGPTIRFAGRVSDHDRSSLLAGAKALIFPNE
                     EDFGIAPIEAMASGTAVIAYRKGGLTETVEEGITGVFFDTQTVDELAELVQKFNPKDF
                     KQSDLVSRAQQFSSAVFRKHFKADVEAAYKTHKAEHHGQN"
     gene            complement(9373..>9853)
                     /locus_tag="VF272_04450"
     CDS             complement(9373..>9853)
                     /locus_tag="VF272_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260151.1"
                     /translation="ILNNIGSHPKLQYYIFHQTTQETGIAASTEQHGTALQEGLKHVE
                     RHPEGQGLGSAGPASFRGDTPIIPESYYLQIAIEAGVLGLVLFCSGQILLGLRLWRLS
                     AGSLGKYHEPWLRPGAGAAVAALTGIALLNLVLHGWTDSSTALIFWSFTGAVIGSKA"
BASE COUNT         2230 a         2544 c         2652 g         2427 t
ORIGIN      
        1 taaaagcaat caagtgttct tttcaaacca ttcctgcggt ctattaaatt gccaggggtc
       61 acgagtatta gacttatgga tggttatatg ccgccataca ccaccaacta tgtaaggctc
      121 tttatctagc cactcttgca attctttctc ggaaggaaag ctaactacgt acattgagcc
      181 tttcgttgaa ccgtcgtcgt taagtagtgc tgcgccgtac caaagatttc ctgactcaag
      241 cagcttatcg cccatagcga tatggtcatg tcgcacagct tgtctgcgag tatgtgcttc
      301 tttgtcagtt ccatctaagc caataattac gaattgcata tagtttaata ataacaaaaa
      361 ggacgactct aagccgtcct agtttgggtg cccaatggta gtcccacggg gaatgtcgct
      421 ggcgcgatct ccactcagcc ttaggcttcg ttacggcgaa ctcggtttcg caaatgcagt
      481 gcgtgcgtga ccagaagaaa aaataccacc gctggtggca tcctttcttc tggtagtccc
      541 acggggaatc gaaccccggt tactggaatg agaatccagc gtcctaaccg ctagacgatg
      601 ggaccataaa acccaaacca aagtttgggt tttgggcggc cgtttggcct gttcttgaac
      661 acatcgttac tgtaacagga ttagagtcgt gagtctagaa gaaatagcgg cctatgctag
      721 ctgcggcttg ctgtagtttt tcggttagcg gacgctgcct ccattccttt aattcaattg
      781 gtttgctgct ttcaagatcg tcttcgaatt gcttggttaa gccggtagcc agcttcatgt
      841 cgtcggccac cataatcgcc tcttcgtcat ggtgaaatga acgcgtattt aaattggagg
      901 atccaatgat gctgagcgtg cggtcgatga tagtaatttt ggcatgtatt ttagtgcgct
      961 gatactcaaa tatatcaacc cctgcttcaa gcaggccatt atagtgatgg cggccggctt
     1021 gcattgcctg ccaatgggac ggatggggac cattggttat aattcgtacg ctcacgcccc
     1081 gcttcgctgc tgcgtgtagg ctacggcgga ctgctgccgt tggtccgaaa taggccgttg
     1141 aaatatcgat tgaaccttta gcctctccta tcaaccgcgt aaaaacgttc gttatatttg
     1201 atcggttagc gggatcgatt ggcgcggtat caacgagtgc caatggaatc gttcccccat
     1261 gtaccggagc cggctcggcc ttttgtaacg gcaattgttc gctactaaac cggttccagc
     1321 tggttatgaa tgaatgcgat agctcactta taaccgggcc ttccagttgg aaatgagtat
     1381 cgcgccaggg ttccggatag ctaggatcat ctagccaaaa gtctgcaata ccgataccgc
     1441 cactaaaacc aatactgttg tcaataatta acaacttctt atgcatgcgc ttattatagc
     1501 gcaaagggtg aagggttttt cgggggttaa accacctaac attaatgtgg tcgagacgca
     1561 ttttcttcag ttgattgcgg tcaaattcat tggatccata ggcatcaata agcagataaa
     1621 tcttaacccc gcgccgggct gcagtgagca gcggctggta aaaaaggtcg gacgcctttc
     1681 ctttttggta gcaaaaatga gcaatataaa ttgaagttgt ggcattatga atggctcgca
     1741 gcataaccgg gtaggcttcg accgcgttca ataaggtggc aaccctattc ccgggtacat
     1801 agttgagctt ggtggtggtt gtcattgcgt aagtatctat gatggcaatt cgtaccgcca
     1861 gtgtcaacgc ggtggcaaca tcccagacaa gtacgccctt tcgggtcgtt tagcgaccca
     1921 ccgaatgtgg acaccgctag gctactatac catcaaaaag cattttgaat acttgaaata
     1981 aaagattttt tggcttgcgt tgggtacagg ttaaaatgtg ggtaatatgc gtgtcaattc
     2041 tgtcaatttt tgtgagtatg ccctattgac ctctttttta agctctggta gtttactgtc
     2101 ctgtgccccg tgtgaggcca atttcttggt tcagggtcgt ggatttacta taaataacga
     2161 aggaacaata tgcgtagatt cgctcttatg ttgctgctga ttgcaggaac agctaactta
     2221 aatgtgggta agatacaagc cacagactcc aagatcaaac caagcaaaat cgccgccgtc
     2281 gccgaagcac caaaagaagc acctcaaaaa cacgtcgttg ccgccggaga atctctttct
     2341 tcaatcgcta ccgataagca gcttgaaagc tggcgcccat tgtggaacgc caacactgaa
     2401 ctgcaaaacc ctgatcaaat caatcctgga caagaactta taatacctaa agatcctact
     2461 gccgaccgtc cccttccgcc tggctatggc gaaccggtgg cggtagctgc acaggccccg
     2521 gctcagcaat cctaccgctc cgctccgcag gctaaggtag ccaacccagc tcccagcggc
     2581 gatattctga gccgaatccg gatgcgcgag tccggcggca actatgccac caatactggg
     2641 aacggctatt acggggcata tcagtacgat gtaggcacct ggaacaacta tggcggctat
     2701 cggtatgctt ccgatgctcc tccggccgtt caggacgcca aggcggccga gacctatgcc
     2761 cgccgcggct gcagcccatg gcccagcact tgctattagg ggcagccacg accgttttga
     2821 tatactggta gaattatgaa gcacagtacg gttgttggaa ttggagggtc gctagttatc
     2881 gcggttgtcg tagtcggctc gacacttgcg tttagccaaa cagccaagaa agcaccaggc
     2941 gccagccaag attcaaataa acccggcatt gaaacacccc caagaacgaa agaacaaact
     3001 atccaaggag atactatgga actacagaaa accgatgaag ttgttggcac cggacccgaa
     3061 gccgtagctg gcaagaaagt taccgtacat tatctcggca cgcttaccaa cgggacgaag
     3121 tttgatagct cctatgaccg caacgagccc ttcggcttta cacttggcgg cggccaggtt
     3181 attaagggtt gggatgaagg cgtggccggc atgaaagttg gtggcaagcg taaattagta
     3241 ataccaccag aaatgggata cggcaaccaa gcggtgagta ctatcccggc taattcaacc
     3301 ttagtgtttg aggttgagct cttgggcgtt gaataaggag cggttgctcg ggctagaaac
     3361 taacgggacg gtcttccggt acaattgaaa accatgtatt ccccttgttg aggggaattt
     3421 ctttacccgc tgtgtcgacc agcttggttc tttcaggata gctagccttg gcccaggtcc
     3481 cttcgatgac gccgccatca cggaaaacca gaaccttatt gctacctaaa atgcccaata
     3541 tagcatgtcc cgtagcatcg tatctaacgg acatatactg cgctacaacg tttttcacct
     3601 gaatttgagc gccggtgtta cggtcgatat gcggcgcgcc agccataaag cggtcgtagc
     3661 agttgcagtc tttattgtag cggaactcta cctggaagcc ggtgtacgag taattaatgc
     3721 cgataacgcc atgcgcaggt gtagccagtg gttgatcttt tttgcgaggg ttaggggtaa
     3781 attgcgccgg gtgagcatat cctcgggatt ccactaagcg gtccatccct tcgctggaag
     3841 tgtaaagatt gtgcggcgcg tagcggtcgc tagctcgggt aaagaactga ttaaccccgc
     3901 cgcccagtgc attcatgctc tttacgttga gcgggcctac ctggctgagg gcttcggcgc
     3961 tgccaccggc atgagcgatt ggcgagttat attccagtgc ccaatcatta aaatatggac
     4021 gcaagctgcg taccggtccg attgatggcg gctgaccttc aacgtagaag gcgagaaaac
     4081 gggtaattcc cccttcagcc agggcttcat aaacaacccc tgcttggctg agccccgact
     4141 gcggacggga ttcggggtga ttctcaataa cgatggctct aattgcccgg tctgccaacg
     4201 ccggctcaag ctctaccccg gttaatggcg aggctttggt ggtaggtacc ggcggcttct
     4261 tttttggtac ttttgcagtg ctcgaaattg gtgtgtccgg cttagtgtac tggatgtaag
     4321 cccatgcgct caccggggca ataatcagca agccagcgac aataataatt ctggtgtgat
     4381 gggttgccaa aaaggcttta aaccggttgt tcgactctgg cttcttcgca ggcggttcca
     4441 ccggcggctc cggctgaggc atctggtttt gactgctgct gatttgaatc ggcacattct
     4501 gcgcctctct ttcacgatca tccatacaac tatggtacca tgccggttac ggtaacgtta
     4561 ctgcattggc gagccgccgc agactggttt cacggccaag cgcatgcaag gtttcgaata
     4621 agcctggtgc cgccgtactg ccagtaatgg caactctaat cagcccaaac ataacgccgg
     4681 tttttaatcc ctgcttttcc accaagtggc gtaaacgttg ttctaaatcg gcctctgaga
     4741 aatcgctatt ctcgagtgca gcaagcgttg cccctaaaat ctgcgtgcgt tggtctttat
     4801 caagctgttt gggaaacagg ttcgggtcaa tcgccggctc agtaaaaaag aagcttgtaa
     4861 gcaagggcag ctcggcaaaa aacttcagcc gttcgtgcac caacgccagt acctgtttgc
     4921 gataggtttc tttagagttc ttggcttccg caggccaaaa tccttgagtt cgctgatata
     4981 gatcatcaag cggcaactgc cggatgtata ccccgttcat ccaaacaagc cgctccggat
     5041 caaataccgc tgggctggat tgaacccggt cgaagctaaa ggttataatc agctcgtcag
     5101 cggtgtaggt ttcctgggtg gtgccgtcat taaagcccag gctggcaaga taggtgacta
     5161 gcgcttccgg caagtatcct gcatcccggt attctaaaac cgacttggcg ccatgccgtt
     5221 tgctgagttt ggccttatcg gctcctagca cctgcggtag atgggcaaat accggcggct
     5281 cccagccaaa ggcctcatag agcagcaggt gcttgggcgt gcttggtatc cactcggctg
     5341 cccggatgac atggctgatt ttcataagat ggtcatcaac aacgttggca aaatgatagg
     5401 tgggataccc gtcggctttc atggctacaa aatcttcaat cacgccgcgc tgccaccgct
     5461 gctcgcccca aactgcgtct tgccagatga catcttcacc gtcggtcact ctaaaacgca
     5521 ttaccggctt ctgattgggc gtggcgggtt ttagtgcggc ctgctcatac ctaaaacgaa
     5581 acggttgctt ggctttctgg gcggcggtgc gcatctgttc taattgctcg ggtgtagtgt
     5641 agtcttcgta gagcacgcca tccttaagca gcagctgagc ctgttcggcg tagagatcca
     5701 agcgttccga ctgaaacacc ggaccttcat cggcctgcat tcctaaccaa tccaggctct
     5761 cggtaatctg ctcaaccgca tctggcacca ggcgctgtcg gtcggtgtct tcaattcgca
     5821 ggataaaact gcccttatgg cgtttggcaa acagatagct aaacaaggct gatcgggctc
     5881 cgccaacatg gaggtagccg gttgggctag gggcgaatcg aacgcgtacc ggttgagtca
     5941 tacgattaag cttagcggga gcagtggctc accgcaacta caggccaagc tgcgtaagat
     6001 cgattgcttc ccaattgctg gatgggttgt ctcggcggta taaggtaact ttgtgcgccg
     6061 tttcgttttc atcatagata tactcaatcc gattggcggt cccttcgcga tggctataat
     6121 gagttttgcg gtcgacaaca gctgggccgg agctgcgctc aagccgaaaa tgctcttctc
     6181 ccctactgaa ctcgaaaaac tcaacgcttt gcataagact tggctggtcg ggcaggggtt
     6241 gcttttcccg cccgtggcgc tcaatgccga gcgtcgcatc gatggagtct accaatcgct
     6301 cccaggcgtt atcgttcaca gcccgtacgc aatcttcaat acttgttcgc ggcccagctt
     6361 ggaggtgccg ataatttgat accaaatgcc gtggttgacc caattagcct ggtcatcatc
     6421 gaggtaaatt accagtcctt gtccctgtac ggccaaaaaa ttggcggttt ggcggctcac
     6481 ataattttcg cgcagcgagt tcgagtccca gttggtgcgg cgctggacaa tgcttaaggg
     6541 cgtctcgctg ctggcgttag taaagttaat gctaatccgg ccgttggagg tattagtcgg
     6601 tccgatttga cggtagctgg aagggatgta ggttggcagg ctggcgtcga tcccggcttt
     6661 ggcggctgcg ttgtggaatg ccagcttcgg cgcgttttgc agccaaataa ggccggacat
     6721 aacaccaatg gttgcaatcg cggctgcaac tttggtaacc attggcacgg tggttgaact
     6781 tttggcggct tttagcgctt gttggagatc gctggattcc ggtgttggaa ccggtgccgg
     6841 ggtggtggcg gccaaggacc caacattcgc ctttacggcg tttggcagcg ggtttggctc
     6901 cggcctagcc agcgccggat gtggcggaag cttttgaatg agcggacttc ttgtatcagc
     6961 agcggggctt ggcggcataa cctgcgctgc tgctggcgcc ggagattgcg gtgcaggcgt
     7021 gggcgtagga gccgccacgg cttcgtagct gcttgaagct gtcgcaggtg atggggggtt
     7081 aggcgggacg ctcggtgcag gccgcaaatc gcgcagagct gcgccggcta tttggcgccc
     7141 gtgaagctgg ccggctggca atggttttgg cgcttgcgga gcagcctgaa ccgctggatc
     7201 tgatcgcaag tccaaggtgc tagtggctga agtaatttcc gacatgctgc tcggagccgt
     7261 tcccgccaca acggtggctc cgcagttact gcaaaaacgt ttgccattta tggctacaaa
     7321 accggttttc ccgcatttgg gacactgcat tgaataaaac ccttatacct cttatgttac
     7381 atcatattag ctgctggtgg gaagagtttt aacctgctta cggctttcac ggtagaccat
     7441 ccacataaaa cgcggtaccg ccagctggcg atgcaggcgt gccggttcca gtagcaggcg
     7501 ccatagccat tcgagaccaa tctgctgaat ccgctgcggc gccttggtcc gtgtgccgcc
     7561 aaagctgtca taatcaaatg ttccgccttc gccaatcaac acgccgtgag caagttgagg
     7621 cgccagtttc gccattaatt cctcctggag cgggaagccc atccctacca aaatgatgtc
     7681 tggtttgcgt tcttgcaggt cggaaagcag cttggcctcc aatggggtgg tttgcagccg
     7741 tttgcgcagc tgctcgcctg ccataccgcc ccattggcca ggccacgtcc cggcgatatc
     7801 cagctggggt agccgagtct ggattgtacg cgcggttgcg ctaatatcac tgccgcgggg
     7861 tgatccaata aggtatacgc ggagttgctg cttcatacag gcttctagga gtttccaggt
     7921 aaaaacgctg ccgccaaact tttctgcaat cggacgttta atggcccggg gacgaacaat
     7981 aatgctggtg gccagaccca aaacccgcac taaacccggc ggtccggcaa gataagccgc
     8041 cgcccattgg gcggaaacgc tttccggcaa ccgcagccag gcctggttta gcagcttgcg
     8101 tacgtccgga tctcggtgtg cccggtcgaa aaattcaacg tatggtttta ccacgtaggc
     8161 aggtggtttt ccgggtgcgg cctggcgcgt gatgagtccg gcggcttgtt ccatagtgag
     8221 cacatcgatg ttcaccccta aaattggtac gctagttttg gccatggtgt tctgctttgt
     8281 gggttttata ggccgcttct acatcggctt tgaaatgctt gcgaaaaacc gcgctgctga
     8341 actgttgcgc ccggcttacc aaatcggatt gcttgaaatc ttttgggtta aatttctgca
     8401 ccagttctgc cagctcatcg acagtctgcg tgtcgaagaa tacgccggta attccttctt
     8461 cgacggtctc ggtaagacct ccctttcggt aggcgatcac tgccgtgccg ctggccatag
     8521 cttctatcgg ggcaatcccg aaatcttctt cattggggaa aataagagcc ttagcgccgg
     8581 ccagtaagct gctgcgatca tgatcgctga cccggccggc aaaccgaatg gttggtccgg
     8641 ctaactgctc caagcgttgg cggtcggcac cgtcgccgat gactaccagt tcaacaccgg
     8701 ctttattgca ggcggcaatc gccacatcaa tttttttata cggcgtcagg ctgctaagcg
     8761 ttacaaaata ggagccctta tgttcgaccg gggtaaagtg ctcgatgtcg gctccgggat
     8821 agatcatcgc atcaactttt acccggtagt atttagcaat gcgcccggct gtcgttttgg
     8881 agttggcaat ccagcggtct actcgagccg cgctcatgta atcccattgg cgcacatttg
     8941 aagtcagcgc atacattacc gctcggcgca gcgggccaac cctttgctcg tgcacgtagg
     9001 ctggccaata gtcccacaca aagcgcattg ggctgtgaca gtaacagatg tggagggttc
     9061 ccggtttggt aatgactccc ttggcaaagg cgctggaaga agaaataacg aggtcgtatt
     9121 cgctgagatc aaattgctca atggcggctg gcagccaggg caaaaggtat cgcggccgtt
     9181 tgcgcaaaaa aaccggccaa cattgcaggc ttgaaggacg gattcgcgtc gaatcaatcc
     9241 gcccgttaaa gacgctgccg tcatacagca gggtgtaaat tggtgcattg gggaagatgg
     9301 tagccatctc catgagcact ttctccccgc ctccttgctt ggtgttaagc cagtcgtgaa
     9361 ctaatgctac cttcatgcct tcgatcctat cacggcgccg gtaaagctcc agaagattaa
     9421 ggcggttgaa ctatcggtcc atccatgtaa aaccagattg aggagcgcaa tccccgttaa
     9481 ggcggccact gcggcgccag cgcccggtcg cagccatggc tcatgatact tgccgaggct
     9541 tccagcgctg agtcgccaca gccgcaggcc aaggagtatt tggccactac aaaacaagac
     9601 cagacccaga acaccggctt cgattgctat ttgcaagtaa tagctttcgg gaataatggg
     9661 ggtatcgccg cggaaggagg ccggcccggc gctacccagc ccttgaccct cagggtggcg
     9721 ttcaacatgc ttcaaaccct cctgaagcgc ggtaccatgt tgttcggttg atgccgctat
     9781 gccggtctcc tgggtggttt ggtgaaaaat atagtactgc aacttagggt gggagccaat
     9841 gttgttaaga atc
//
LOCUS       DASVGB010000141         5336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_242117, whole genome shotgun sequence.
ACCESSION   DASVGB010000141 DASVGB010000000
VERSION     DASVGB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242117"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VF272_04455"
     CDS             complement(<1..830)
                     /locus_tag="VF272_04455"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX7260152.1"
                     /translation="MIPLFPHFTPLALELKDEIQAITAHYPSYSDYGFTSMWSYDTES
                     SARICLLNQNLVLRYGDYITGDYFYSFLGAAEIDATAKTLIAYAAEHKMDTTLKLIPQ
                     IVIDHFKHPEHFVISEDRDNFDYILSVPELVAMHGPHYQNKRNLVRRFLRAYEHEMKT
                     KPLELSDATVRDEIIQTFHDWAASRNKSSEEIAVELEAIKRMLNDHDKFDLNTIGIFI
                     NNRLRGFSIFEFVHDGHGVVHYEKADVGFEGIFYYLKQQAAVAMHSNGVSHLNYMQDL
                     G"
     gene            848..1738
                     /locus_tag="VF272_04460"
     CDS             848..1738
                     /locus_tag="VF272_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7260153.1"
                     /translation="MTDWLIPALLAPMLWAFVVLIDDNLLRNVFRNAYFGGIAAGLFA
                     LIPLLILLVLPHTIPAWPVLVAAIAAGLATTCFYFLLFRSLELAEPSIVTAISMLATA
                     FVPVMAWLVVGEQLGVYQLIGLAVLVVSSVAMSLLDARGFRPNRALYIMAGGSVLYAA
                     AAVAMKYVYGEVDYRSGYLFFAAGMVLGGLFLMTATKPGRGFIATARGFSLNVWGFIV
                     VGELLNVCAEALQGLAVSRGPVSLVRALEGLQPAWVLLIALILFPFWPRYFREAAGAH
                     RGAKFACFAVIIVGVIMVAR"
     gene            1881..2621
                     /locus_tag="VF272_04465"
     CDS             1881..2621
                     /locus_tag="VF272_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260154.1"
                     /translation="MNVDSHDSSNPYNAAYTLTYGLLALFGGVGGLIVAHRWGGFKSL
                     IGRALSAFALGLLAQEFGQLVYNYHIYVLDTEPYPSLGDVGFFGSVLFYIYGIWLLAQ
                     AAGLRVALKSHRNKLLLIVIPLIFLSISFSVFLREYEFDWSNPLTIFLDFGYPVFQTL
                     YVSIAALTFIVSRKYLGGVMRPAILMLAGALTLQYICDFTFLYQVSHEAWYAGGWNDY
                     LYTLSYAVMGLSLLRFGTVFSEIRKIDR"
     gene            2621..2899
                     /locus_tag="VF272_04470"
     CDS             2621..2899
                     /locus_tag="VF272_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260155.1"
                     /translation="MDIFAQAAKSIISQQEAIMGPLALEQAKKVQGLVIDKASHDVMI
                     EGDKTTALQHLVEQYKQLFGQTAVEVCRQAAKDFMKQVPPSQVPELLR"
     gene            2956..4362
                     /locus_tag="VF272_04475"
     CDS             2956..4362
                     /locus_tag="VF272_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7260156.1"
                     /translation="MSDPTQPAPAEIQARSDQLTHIAQEMYKKNMELAETNHTLMLLR
                     RIDDIVLSSVSDKGVVLRRVAEAISQDSDFSFSMIYLRNKQKRSLVPGALVSGSKDEK
                     LEKLAQTTLLHHAVTLRHTANPMARTVEVPFIASTTRLYEIVQPNLEFSEADELQKQL
                     KLEQFFICPLIARGAVLGTMVLGTTLTSEALSFYQKSLLERLTVAVGLALDNTLLYTE
                     AQESSRRLQVANRHLKELDKAKDEFISLASHQLRTPLTSIKGYVSMLLEGEAGAITKE
                     QHEFLDYAYRGSQRMVSLISDLLNVSRMSAGKFYIEREPLDIAAIVEEEIQQLQQHPD
                     AKKLKLTYVAPKKPLPLLNLDENKTRQVIMNLIDNAIYYTREGGVTVMLAQEGQHAVL
                     RVSDTGIGVPKTARAKLFGKFFRANNAQTIRPDGTGLGLFLAKRVIEEQGGTILFESV
                     EGKGSTFGFSLPLKEKGN"
     gene            4376..4798
                     /locus_tag="VF272_04480"
     CDS             4376..4798
                     /locus_tag="VF272_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7260157.1"
                     /translation="MTDFYENDNAPAEVPMNGKTILLAEDDPFISRMYEAKLTNAGYH
                     VVLVHNGRDAYDRIKSDNPDLIMLDISMPELTGFEVIKALQANGLEDMVQRIMVLTNS
                     ADPRNQARARELNIDYIVKADLTPQEVLVRINKKLQVG"
     gene            complement(4803..>5336)
                     /locus_tag="VF272_04485"
     CDS             complement(4803..>5336)
                     /locus_tag="VF272_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7260158.1"
                     /translation="IATKDKVDRRLKEMAREIIARYKQKDPLFVCLLRGGAPFTTKLL
                     FEVAAQAPDFHPELDYLTVKTYSNQLTSKTPEVVMDLAPDTITEGRCIIILDDILDEG
                     HTATFVTKHLLKHGAAAVDLVVLVQKQKDRSTYPAASLYGFEASDGWIIGMGMDDPRV
                     KKEAHRWDDYIGITTTN"
BASE COUNT         1279 a         1211 c         1368 g         1478 t
ORIGIN      
        1 cctaaatcct gcatataatt cagatgggag acgccattac tatgcattgc cacggcggct
       61 tgttgcttga ggtaataaaa aattccttca aagccaacat cggctttttc ataatgaacg
      121 acgccgtggc cgtcatgaac gaattcgaag atgctaaagc cgcgtagccg gttattaata
      181 aaaataccta tggtattaag gtcaaatttg tcatggtcat tgagcatgcg cttgattgct
      241 tcgagctcca cggcaatttc ttccgagctt ttgttgcgcg acgccgccca gtcatggaag
      301 gtttggatta tttcatcccg aactgtagcg tcgctaagtt cgagcggctt agttttcatc
      361 tcatgttcgt atgctcgtag aaaacggcgg acaagattac gtttattttg atagtgagga
      421 ccgtgcattg caacgagctc cggcactgaa aggatgtagt caaaattatc ccgatcctcc
      481 gaaataacaa agtgctcagg atgcttaaag tggtcgatta caatctgagg aatcagcttt
      541 aaggtagtat ccatcttatg ctcagcggca taggctatga gcgtcttggc ggtggcgtca
      601 atttcggctg ctcccaggaa gctataaaaa taatccccgg taatatagtc gccgtagcgc
      661 agtaccagat tttggtttag aaggcagatt cgggcgctgg actcagtgtc gtacgaccac
      721 atgctggtga aaccgtagtc tgagtatgag ggatagtgcg cggttatggc ctgaatttcg
      781 tctttaagct cgagtgccag gggagtgaaa tgtgggaata gcggtatcat agtgatatta
      841 tacgacaatg accgattggc tcattccggc tttgctggcc cctatgctat gggcatttgt
      901 tgtactgatt gacgacaacc tgcttcgaaa tgtgttccgc aacgcctatt ttggcggtat
      961 tgccgcagga ctcttcgcac ttatcccact actcattctg ctggttttac cgcataccat
     1021 ccccgcctgg ccggtgctgg tggcagcgat tgcagcgggc ttagcgacaa cctgctttta
     1081 ttttctcctt tttagatcgc tggaacttgc cgagccttcg attgttacgg ctatatcaat
     1141 gttggcaacg gcttttgtgc ccgtaatggc ctggctggtg gttggagagc agcttggtgt
     1201 ctatcagcta atagggctgg cggtgctcgt ggttagctcc gttgcaatgt ccttgcttga
     1261 tgcgcgtggt tttcgaccca atcgtgcttt atacataatg gccggaggtt cggttttata
     1321 tgccgccgcg gcggttgcaa tgaaatacgt atacggtgaa gtcgactatc gaagcggtta
     1381 tctctttttt gcagcgggaa tggtattggg cggtttattc ttgatgaccg caaccaagcc
     1441 gggccgcggt tttatagcta cggctcgggg tttttctctt aacgtatggg gttttatcgt
     1501 tgtgggagag ttattgaatg tctgtgcaga agcgttgcag gggctggcgg tcagccgggg
     1561 accggtttcg ttggttcggg cgcttgaagg attgcagccg gcttgggtgc tgcttattgc
     1621 cctgattcta tttcctttct ggccgcgtta ttttcgtgaa gccgccgggg cgcatagagg
     1681 tgctaagttt gcctgctttg ccgtgataat tgtgggcgtc attatggttg cacgctaacg
     1741 catgcaagca agaaggttga cataaaacgc ttatgcttag atactgaagc tatatgctat
     1801 ccgcgctaag aacgaaccga ttttctcaac tgctggcatt tttattgctt gggtttagtg
     1861 catggtggtt atggatttac ttgaacgttg attcgcatga tagcagcaat ccctataatg
     1921 ccgcctacac cttgacctat ggcttgctgg cactttttgg gggcgtcgga ggcttaattg
     1981 tcgcacaccg gtgggggggc tttaaaagcc tcattgggag agcactttcc gcttttgcac
     2041 tgggtctatt ggcacaagag ttcggccagc tagtatataa ctatcatatc tatgttttag
     2101 atacagaacc gtacccttca ttgggtgatg tcgggttttt cggcagtgtc ctgttttaca
     2161 tctatgggat ttggttgctc gcccaggcag ccggattgcg ggtggcctta aaatcccatc
     2221 gtaataaact gcttcttata gtgattcctt tgattttctt gagcatttca ttctcggtct
     2281 ttttacgaga atacgagttt gattggtcta atccgctcac tatatttctg gatttcggct
     2341 acccagtttt tcaaacgctc tatgtctcaa ttgcagcctt aacattcatc gtatcgcgca
     2401 agtatttggg cggcgtaatg cgaccggcca ttttgatgtt ggcaggtgcg ttaacgttgc
     2461 agtatatttg tgacttcacc ttcctgtacc aagtaagcca cgaagcctgg tacgctggag
     2521 gttggaacga ttatctttat actctttcgt atgcggtcat gggtctgagc ttactgcgct
     2581 tcggaacggt attctcggaa attcggaaga tagataggtg atggatatct ttgcccaggc
     2641 cgctaaaagc atcatttcac aacaggaagc tatcatgggt ccattggctc tagagcaggc
     2701 taaaaaagtc cagggtctag tcattgataa agcgagtcac gacgttatga ttgaaggcga
     2761 taaaaccacc gctcttcagc atctcgttga acagtataag cagttgttcg ggcaaacagc
     2821 ggtagaagta tgccgccaag ccgccaagga ttttatgaag caagtaccgc cttctcaggt
     2881 tcctgagttg ctgcgctaga tcgtccatcc tgtccccgca tttaccaccc taagcataac
     2941 cgttatactc taactatgtc cgatccaacg caaccagctc cggcagaaat tcaggctcgg
     3001 tctgaccagc ttacccatat tgctcaagaa atgtacaaga agaatatgga gttggctgag
     3061 accaaccaca ccttaatgct gcttcgccgc attgatgata tcgtattgag ttcggtaagc
     3121 gataagggcg tcgttttgcg gcgagttgca gaagcgatat cccaagactc cgatttcagc
     3181 tttagcatga tatatttacg gaataagcag aagcgttccc tggttcccgg cgcattggtc
     3241 agtggaagta aagatgaaaa gcttgaaaag ctggcccaga ctacactgct tcatcatgct
     3301 gtaaccttgc gtcataccgc gaatcccatg gcacgtacgg ttgaagtgcc atttattgct
     3361 tcaaccacgc gtctgtatga gattgtgcag cccaaccttg agttttcgga ggccgatgag
     3421 ctccaaaaac agttaaagct tgaacagttc ttcatttgtc cgcttatagc tcgcggagcg
     3481 gtcttgggta cgatggtact gggaaccacc cttacatccg aggcactgtc gttttatcag
     3541 aagagtctgc tagagcgcct caccgtagcg gtagggcttg ccctggacaa taccttgttg
     3601 tataccgagg ctcaagaatc atcaagacgg ctacaagttg ccaaccggca ccttaaggaa
     3661 ctagataagg caaaagatga gtttatttcc ttggcttcgc atcagttgcg cacaccgctc
     3721 acaagcataa aggggtatgt ttcaatgttg cttgaaggcg aggcgggggc cattactaag
     3781 gagcagcacg agttcttaga ctatgcctac cgtggttcgc agcgtatggt aagccttatt
     3841 tccgacttgc tcaacgtgtc aagaatgtcg gctgggaagt tttacattga gagggaaccg
     3901 cttgatatag ctgccatagt tgaagaagaa atacagcagt tacagcaaca tcctgatgct
     3961 aaaaaactca agcttaccta tgtagcgcct aaaaaaccgc ttcctttact caacctcgac
     4021 gagaataaaa cccggcaagt tattatgaat ttgattgata atgccattta ttacacccgg
     4081 gagggggggg taacggtgat gcttgcacaa gaagggcagc atgctgtgtt gcgggttagt
     4141 gatacgggga taggcgtgcc aaaaacggca cgggccaagc tattcggcaa gtttttccgg
     4201 gcaaataatg cccaaaccat acgcccagac ggcaccggac ttggtttgtt cttagctaag
     4261 agggttattg aagagcaagg tggtacaata ctgtttgaaa gtgttgaggg caaagggagc
     4321 acctttggct tctcgttgcc gcttaaggaa aaggggaatt aagagttctt gacctatgac
     4381 tgatttctac gagaatgaca acgctcctgc cgaagtccca atgaacggca aaacaatatt
     4441 gcttgcagag gatgacccct ttatttcacg gatgtatgag gctaagctaa ccaatgccgg
     4501 gtatcatgtt gtgctggttc ataacggacg ggacgcctat gaccgtatca aaagcgataa
     4561 tcccgacttg atcatgcttg acatcagtat gcctgaatta accggttttg aagtcattaa
     4621 ggcgcttcaa gcaaatgggt tggaggatat ggtgcagcga attatggtgc tcaccaactc
     4681 ggccgaccct agaaaccagg caagagcccg ggaactaaac attgactata ttgtgaaggc
     4741 cgacctaact ccgcaagagg ttctggtgcg gatcaataaa aaactacagg taggttaagt
     4801 ttttaattcg tggtggtaat tccaatatag tcatcccagc ggtgcgcctc ttttttaacg
     4861 cgcggatcgt ccatgcccat cccaataatc caaccgtcag aggcctcaaa gccatagagg
     4921 ctggctgccg gatacgtgct gcggtctttt tgcttttgca ctaaaaccac caaatctacg
     4981 gcggcagcac catgtttcag caaatgctta gtaacgaacg tggcggtgtg cccttcatct
     5041 agtatgtcat ccaaaataat gatgcagcgt ccctccgtga tggtgtcggg tgctaagtcc
     5101 ataacaactt cgggcgtttt gctcgtaagc tggttgctat aggtctttac cgtcaagtag
     5161 tcgagctcag gatggaagtc cggtgcctgg gcagccacct cgaatagcag tttagtggta
     5221 aaaggcgctc ctccgcgcaa gaggcacaca aacagcggat ccttttgttt gtagcgcgca
     5281 ataatttcac gcgccatttc tttcaggcgc cgatcaacct tgtctttcgt tgcaat
//
LOCUS       DASVGB010000142         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_72273, whole genome shotgun sequence.
ACCESSION   DASVGB010000142 DASVGB010000000
VERSION     DASVGB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72273"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3305)
                     /locus_tag="VF272_04490"
     CDS             complement(<1..3305)
                     /locus_tag="VF272_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260159.1"
                     /translation="MNGVALLVIIFVVFAVVLATIAFLAYRRILRRAKGVERGLKMVP
                     LLIHLPPPSDDTENSGNRDVRDIMREKISQAEVLYDLIAGTAQAGFKSNFYGQRHIAF
                     EIIASNGLIHYFTAVPVALVSIVEQAIVTAYPGARLEEVEDHNIFNPQGHLTGTVGGE
                     LVLKHKAAYPIGTLDQMERDPMVGLLNSLSKLELGDGAAIQIMLRPAHEGWTKNSIKL
                     VDNTRHTRQGGLGFSAADIAKGAVKTSEEWRSDAKTFELSKLEEDTMQRIEEKTKHHG
                     YEILIRVLASSPSYDRSQILLRNIITSFSLFEAPGSNGFQFIEGRDMQGLVTAFIFRF
                     FPPELSSNILNSVELATLFHLPDAQFTPSTQVERQRSKQVDGPVSMSENGLLFGYNFY
                     RGVKKEVRLGPEDRRRHTYIVGQTGTGKSTVLENLAVQDMLAGNGFAFIDPHGDTAEK
                     LISMVPKERAEDVVYFNPGDMEHPLGLNLFEFTDPQQKDFLVQESINMLYKLYDPGKT
                     GIIGPRFEHWFRNAALTLMSDPNGSSFVEIPKVFTDTAYLKNKFKYLNDPTVIEFWTK
                     EMAQTSDYHKSEMLGWFVSKFGAFLSNEMMRNIIGQTHSGFNIREIMDNKKILIVNLG
                     KGVVGEQNSQLLGMIFVIKFQAAAMSRSNIPEDQRQDFSLYVDEFQNFSTESFASILS
                     EARKYHLNLIVANQFIGQLTDEIRDAVFGNVGTILCYRTGPEDAEFLVKQFEPIFDQR
                     DLVNMPNYNAIMKLLIGGLPSQPFSITMLPSLGQPHVEMGTAIKQLSAAKFGVPRAVA
                     EADIFARLKNTPPLPAAPIPSPVAALPQQPAAPVSQIAPVPSAVQPSAPMLPPAQTSG
                     STAAPEPVQMMKTEKTIAPHDGLSLSQLRASSPAPPVNASAPIRAPQAQAPAQSSAPM
                     QAPTPAAPTPVPAQAVSPPPTPVPAPTPVVSPPVVSTAPINPSPPQPSQKLDIVQTAV
                     SKLPTVQPTPNSQAAPVPKPRLEPPKDESKKLVLRIEAHETDGRRDDKPVKPPVLRIE
                     AHETDGKVDLPPVSKTSAPSPSVPVPVPVSVSAPKASLAPSPTSPPPALKPVLPPEEV
                     KPAPPAPAPTPAPP"
     gene            complement(3362..3787)
                     /locus_tag="VF272_04495"
     CDS             complement(3362..3787)
                     /locus_tag="VF272_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260160.1"
                     /translation="MQPNQNANIQPTPNQPLAPEQLPPSGVEQQPAPVAPEAAPNAPQ
                     AGPQAQPIQLPQIPDVPAQQLPVSPATNTSTPAAPVPTPPPVVANDVDVIEKEWVDQA
                     NKIIEQTKNDPYTEEEAVEALQIDYLKKRFGHDVKKPDA"
     gene            complement(3922..4437)
                     /locus_tag="VF272_04500"
     CDS             complement(3922..4437)
                     /locus_tag="VF272_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260161.1"
                     /translation="MSSLEEVVDPDETISLIIHRHWISLVFPLFSALLVGMVGVLLFY
                     LLGRFPERLQAIGPLSMVVLAGAAIILLSVVLIIANIKIYARNGLILTDKILYLSRQT
                     SLINRNTAQFNLHKLEDVSASQRGFLGTLFDYGDVTAKTSGEEKNMVFAWAPHPQRIA
                     ETILDAHEKVK"
     gene            complement(4495..>5017)
                     /locus_tag="VF272_04505"
     CDS             complement(4495..>5017)
                     /locus_tag="VF272_04505"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7260162.1"
                     /translation="GLQLDQDALAVAIASGVGSKQPVITAAPTKKVPFQTEYNRSVSL
                     DYGKYIEINLNQQHLWVYQDHKVIYESAITSGARGSGYPTVTGLFSVQAKQTNRNLNG
                     YAIGYNYNVFVKYWMPFFGNYGLHDASWRNGVFGGQDYVYNGSHGCVNLPEATAAFLF
                     GWADVGTPVWVHP"
BASE COUNT         1247 a         1179 c         1308 g         1283 t
ORIGIN      
        1 ggaggtgccg gcgttggagc tggtgccgga ggtgccggct tcacttcctc gggtggaagg
       61 accggtttca atgctggcgg aggtgaagta ggcgatggcg caagtgatgc cttaggagct
      121 gaaactgaga ccggcacagg cactgggacc gacggacttg gggcggatgt ctttgagact
      181 ggaggcagat cgactttgcc gtcagtctca tgagcctcaa tacgcaacac tgggggtttc
      241 acaggtttgt cgtcacgacg accgtcggtc tcatgggctt ctatgcgtag aactagcttt
      301 ttggattcat ccttaggagg ttcaagacga ggcttgggaa caggagctgc ctgagaatta
      361 ggggtcggtt ggaccgtcgg caattttgat accgctgttt gaacaatatc cagtttctgg
      421 gaaggttgag gcggagacgg attgatcggc gcagttgata caaccggtgg cgatacaaca
      481 ggggttggag ctggtactgg ggttggcggc ggcgagactg cttgggctgg tactggagta
      541 ggtgctgctg gtgtcggagc ctgcattggt gccgaggatt gggctggtgc ttgtgcctgt
      601 ggcgctcgaa tgggggctga agcgttaaca ggaggtgccg gacttgaagc acgcaactgg
      661 ctcaaggaca gtccatcatg tggcgcaatg gttttttcag tcttcatcat ctgcactggt
      721 tctggagcag ctgtggatcc tgaagtttga gccggcggca gcattggtgc gcttggttga
      781 accgccgaag gcacaggtgc tatttgagac accggtgctg ctggctgctg gggtaatgcg
      841 gcgacgggac ttggtattgg tgcggctggc aatggcggcg tattcttcaa ccgagcaaat
      901 atatcagctt cggcaaccgc ccggggcacg ccgaacttgg cagcgctcag ctgtttgatg
      961 gcagtaccca tttctacgtg gggctgaccc agtgatggca gcattgtaat actgaatggc
     1021 tggctaggca ggccgccaat caagagcttc attatcgcat tatagtttgg catgtttact
     1081 agatcccgct gatcaaaaat cggctcaaac tgtttcacta aaaattcggc atcttcaggc
     1141 cccgttcgat agcagagaat ggtaccaacg tttccgaata ccgcatcgcg gatctcatca
     1201 gtaagctggc cgataaactg gttggctaca atcaagttca aatggtactt acgagcctca
     1261 cttaaaatac tggcaaagct ttcggtgcta aagttctgga actcgtctac atagagcgaa
     1321 aagtcttgcc gctggtcttc tggaatgtta gaccggctca tggcggcagc ttgaaacttg
     1381 attacgaaaa tcattcccag aagctgagag ttttgctccc ctactacacc ttttccaaga
     1441 ttaacaatca ggatcttctt gttatccata atctcgcgga tattaaaccc ggaatgggtc
     1501 tggccaatga tattacgcat catttcatta ctgaggaacg caccaaactt actgacaaac
     1561 caaccgagca tttctgattt atggtagtcg ctggtttggg ccatttcctt ggtccagaac
     1621 tcaatcaccg tcggatcatt aagatatttg aacttattct ttagataagc cgtatccgta
     1681 aatacttttg gtatttcgac aaaactagaa ccgttcggat cagacatcag agtcagtgcg
     1741 gcgttccgaa accaatgctc aaagcgcggc ccgataatac cggtctttcc aggatcatac
     1801 agcttataga gcatattgat agactcttga accaggaagt ctttttgttg cggatcggta
     1861 aattcaaata agtttaatcc cagcggatgc tccatatcgc ccggattaaa gtacacaacg
     1921 tcttcagctc gctctttagg aaccatgctg attagttttt cggcggtatc accgtgtggg
     1981 tcaataaaag cgaagccatt gccagccagc atgtcctgga cggccagatt ttctaaaaca
     2041 gtcgatttac cggtaccggt ttgaccgaca atataggtgt ggcggcggcg atcttcaggt
     2101 cccaagcgca cctctttttt cacgccgcgg tagaagttgt aaccaaatag cagaccgttc
     2161 tccgacatac ttaccggacc atcaacctgt tttgagcgtt gtcgctctac ctgggtgctt
     2221 ggggtaaatt gagcatccgg caaatgaaac agcgttgcca gctcgacact attgaggata
     2281 ttgctcgaaa gctccggcgg gaaaaagcga aagataaagg cggttaccag tccctgcata
     2341 tcgcggcctt cgataaactg gaagccgttg gagcctggag cctcaaacag tgaaaatgag
     2401 gtaatgatat tgcggagtag tatttgcgaa cgatcataag acggcgatga ggcaagcacg
     2461 cgaatcagaa tttcatagcc gtgatgcttg gttttttctt caatccgctg catcgtatct
     2521 tcttcaagct tgctcagctc gaacgtttta gcatcggaac gccattcctc cgatgtctta
     2581 accgccccct tggcaatgtc ggcagcacta aaccctagtc cgccttggcg ggtatgacgg
     2641 gtattatcaa ccagtttgat tgaattcttt gtccagcctt cgtgggcggg acgcagcata
     2701 atttgaatag ccgccccatc acctagctca agcttagata atgagttaag caaccccacc
     2761 attgggtcgc gctccatttg atccagcgtt ccaattgggt aggcagcttt atgcttaagt
     2821 actagttctc ctcctaccgt tcccgtgagg tgtccctgag gattaaagat attgtgatct
     2881 tcgacttctt cgagccgggc gccgggatag gccgtcacaa tggcttgctc gacaatactc
     2941 accagggcaa ccggcacagc cgtgaaataa tggatcaacc cattcgaggc aataatttca
     3001 aaggcaatat ggcgctgccc gtagaagttg cttttaaacc cggcttgagc ggtccctgct
     3061 ataaggtcat acagcacttc agcctgagat attttttcgc gcataatatc ccgaacgtcg
     3121 cgatttccgc tgttttcggt gtcatcactt ggaggaggca gatgaataag cagcggcacc
     3181 atcttcaaac cacgttctac gcccttagcc cgtcgaagta tacggcggta cgccaaaaaa
     3241 gcaattgtgg ctaacacaac ggcaaacacg acgaagatta ttaccagaag ggctactccg
     3301 ttcatctcct atgaccatcc gaattcatgt cagtgctcac catcccgtga cttgcggacg
     3361 cctaggcgtc cggcttttta acatcatgcc cgaaacgttt tttgaggtag tcaatttgca
     3421 gtgcttcaac cgcttcttcc tcggtgtatg gatcattctt tgtttgctca attattttgt
     3481 tcgcctgatc aacccattct ttttcaatga catcgacgtc gtttgctacg accgggggcg
     3541 gtgtcggtac cggtgcggcc ggggttgaag tgttagtagc aggagaaacc ggtaattgct
     3601 gagctggaac atcaggaatt tgaggcagct gaattggttg agcttgtggt ccagcttgcg
     3661 gggcattggg cgcggcctca ggagctactg gagcaggctg ctgttcgacg ccagagggcg
     3721 gcagctgttc cggtgcaagc ggctgattag gggtaggttg gatattggcg ttttggttag
     3781 gttgcatatg cgttcctttc ttgctgaaat tataccactt atggttaaaa tccaccagat
     3841 tcgatgaggt ggaattagta tcagtttaaa aaaagtggta taaaaggcag tttttgatgt
     3901 aatcattaca tcaaaataca gctatttgac cttttcgtga gcatccaaaa tcgtttcggc
     3961 gatgcgctgc gggtgcggag cccaggcaaa aaccatattt ttctcttcac cggaagtctt
     4021 ggccgtaaca tcaccgtaat caaaaagagt accgagaaat ccgcgctggg aagctgaaac
     4081 gtcttctagt ttatgcaaat taaattgggc cgtattgcgg ttaatcaggc tggtctggcg
     4141 cgacaagtaa agaattttat cagttagaat caacccattg cgagcataaa ttttaatatt
     4201 agcgataatc aagacaactg agagtaagat gatagccgct ccggctaata caaccataga
     4261 cagtggaccg attgcttgaa gtcgctcggg aaaacgacca agaaggtaaa atagcaacac
     4321 gccaaccata ccaacgagca gtgcactgaa aagtggaaac accaagctta tccaatggcg
     4381 atggattatg agggatatgg tttcgtccgg gtcaacgact tcttcgaggc tagacataat
     4441 gcacttcgct tatagcttct atgctagcac agctaagagc aggacgataa tggcttaggg
     4501 atggacccaa accggcgtgc caacgtcggc ccacccaaac aggaatgcag cagtagcctc
     4561 gggaagattc acgcaaccat gcgagccgtt ataaacgtaa tcctggccac caaatactcc
     4621 attgcgccaa ctggcatcgt gcaggccata gttaccgaag aacggcatcc agtattttac
     4681 aaaaacgttg taattatagc cgattgcata accattgagg ttgcgattgg tttgcttggc
     4741 ttgcaccgaa aacaatccgg taacggtagg atacccggaa ccgcgggctc cactggtaat
     4801 cgcagattca taaataactt tatgatcctg atagacccaa aggtgctgtt ggtttaagtt
     4861 aatttcaatg tacttaccat aatcaaggct aacgctacgg ttatattcgg tttggaaagg
     4921 cactttcttg gttggggcag ccgtaattac gggctgctta gacccaacac cactggctat
     4981 tgcaaccgct aacgcatctt ggtctagctg caagcca
//
LOCUS       DASVGB010000143        13122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadia bacterium isolate
            SMAG_U2104 k141_243715, whole genome shotgun sequence.
ACCESSION   DASVGB010000143 DASVGB010000000
VERSION     DASVGB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961423
            Sequence Read Archive: SRR25158495
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadia bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadia bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:05:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 913
            CDSs (total)                      :: 872
            Genes (coding)                    :: 871
            CDSs (with protein)               :: 871
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Candidatus Saccharimonadia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243715"
                     /isolate="SMAG_U2104"
                     /isolation_source="soil"
                     /db_xref="taxon:2890869"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="28.498423 N 119.904275 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VF272_04510"
     CDS             <1..304
                     /locus_tag="VF272_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX7260163.1"
                     /translation="ANFVTPTELADGVYFGFSDLSNYRHHPSIIFIGIPTTMGNTQRR
                     IETYLLDIPDKDYYQLPVTIVLEYFHRTNQTFDSVNALMAAMKTDEATMRKWLSTL"
     gene            complement(290..889)
                     /locus_tag="VF272_04515"
     CDS             complement(290..889)
                     /locus_tag="VF272_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260164.1"
                     /translation="MKSFAIDQKIAPLANQYYVYELDVSGEKGKLLAFAHQKRFAWKE
                     KFTFYADESKQKMLMEVQARQVMDFGARYDVRDAQGTVLGVIGKAFGASFLRSTWHVF
                     TPDNETIPLVIARERSQALAIFRRLWELLPLIGEIPFFIKYHFELINPSDQTVYGSYE
                     KVTTLRDHYRLSADDQLLQKTDWRVLVSLGIMMDALQGR"
     gene            complement(1086..1499)
                     /locus_tag="VF272_04520"
     CDS             complement(1086..1499)
                     /locus_tag="VF272_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260165.1"
                     /translation="MDQQTRITGIRPLALAIFEGTLGLALGILLAVAVFIESAALQTA
                     LTNSLLQGLIFGLSASLLTLLLVPLFYFAIGFVAGYLHALLFNALAAGSKEMVVTMEP
                     DEFEDLPSQPITRREHAMGFGERMPERRRPRSNRR"
     gene            complement(1634..3304)
                     /locus_tag="VF272_04525"
     CDS             complement(1634..3304)
                     /locus_tag="VF272_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260166.1"
                     /translation="MKRFMGIMVVIAAALGMAVGSSTQPTQAAGGASFYASPTATTYQ
                     VGDVIRVAIMINSAGQAINAGEATITWTPGLQYSFVSTASSIFTTWTSGGGAGPVGSV
                     SSVYFSGGLSNPGYNGSAGRVVTVIFNATQPGTYSVHVAGSQILANDGMGTNILCCSS
                     GTTFTVAAKAPPQPLLSVVSKTHPNQNQWYKAHDVNVNWYATTAIGNYSFKFDHSPTG
                     NPTTPNNATIQTTKTLEDGLWYFHLKGSNTSGSATVHFAIRIDSKPPGNFAVIPDNGG
                     NSSNPTPKMNFNSVDATSGVERYTASIDGGPAFPMASGDAVPRQKPGEHTIVVTAHDR
                     AGNTTEASLSFKVEGITPPTIVSGPTSLVIQKPMKFIGQSSATDTIFVYLNGKLVDQF
                     IAKTKQVAKSKSKGGGGFPEGITWEYRYNAELFPGSYKVFFSRTTSVGAESEPTKVRT
                     VTITATAAQQSFGKSINLSIAVLQWLLIIILLILNIIFYSLWRYYTYLLKKGGNDAPL
                     HDPLHEALREVAAWRPFGWLVNGTLKAQRGFMRLGPLRRRKQEESAGK"
     gene            complement(3301..3831)
                     /locus_tag="VF272_04530"
     CDS             complement(3301..3831)
                     /locus_tag="VF272_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEX7260167.1"
                     /translation="MNPNETKIMASTAKLRIIGLLLVVALFVVGLAVIMQTTKPRKAS
                     IGASETTAQTGHQLKVPIEIDTGGLTINAAEVNLRFDNALVEVVRVSKEQSFFTLWID
                     DQPQFSNEAGTISFAGGVFSPGFKGKAQVGAVILRSKRPLTTTLAFASGTQVLLNDGK
                     GTAVPLELSPITVEMP"
     gene            complement(3869..4681)
                     /locus_tag="VF272_04535"
     CDS             complement(3869..4681)
                     /locus_tag="VF272_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260168.1"
                     /translation="MRRAVAFILLLASLIGLSGWSHTSFTASSESDVGAELPVQTGGI
                     RFVGEASPSALVTFQRNGSVIGTQVAGSDSSFDMTFTTQPLGSATYSIYATDLSSRTT
                     AEITTIATILPAFTTTLSGYLLPTTFSLALNPIKRPQTQVASGTARHNSTVTTLWSGT
                     KYSDSFTTQDATSASGNWNGSPSQTLHLGTYTTSAVVQAPGGALSPQSPDTSFQVVLS
                     ADLNNDTKINLTDFSILMFFYLQININKPADINDDTKTNLTDFSIMLFFWTG"
     gene            complement(4687..5700)
                     /locus_tag="VF272_04540"
     CDS             complement(4687..5700)
                     /locus_tag="VF272_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260169.1"
                     /translation="MIQNFMRVAIGVAIAASFGTLNHVQAATPLGLTVSPPTYELSAN
                     PGDSLNNTIRITNDSDSAVTLQVSTQDFTVGGTEGSVSVLNDSVDPAAFSKWFRFPTT
                     QLQLAPKASALVPFTITVPKQAEPGGHFATVLFNPVVTANATSTGARVIQRVGSLILM
                     RVSGAIKEQGSIESFHTKTFKGSWQSITGSDGKTKILVASAEDLRGEHKQSFFSKGPI
                     AFDILFKNSGNVHFKPAGTVTIYNLFGKKVDQLAINPRNVFPGGERRITVIWPKTDLW
                     GGYYSAHAAAVYGSENKILTAETKFWAFPLIPFLIILVVLVLLIVLRHRLLNAARVLI
                     RGR"
     gene            complement(5728..6828)
                     /locus_tag="VF272_04545"
     CDS             complement(5728..6828)
                     /locus_tag="VF272_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260170.1"
                     /translation="MRNRRHTFTARLHAVIALFFAVGASKVLWMATIAYAGSLSSATI
                     LLSDPRPAQNGTTYTFNFSVASTTVLKAFRAQMCTTPSGSCTTPAGFSGSFALLASQP
                     TGFGDGSGWVSDPVTGSLQMVKAGNVAAPSGSQQIIFSNVTNPASEGTYYMRLTSYSD
                     ATYATDVDNGTVALTIVSGITVSATVDPTLNFSVSGIPASTVYKGALNTSDRCVDSAT
                     SISFGTPLLPLSPDTNYDCGQTLTTSTNGNGGYQVTVNTIDPGNTLKNGPSSLPDWTG
                     TNASPTATPGGTAEIFAYTTSDSSLSGSANRFSTSDNLFAGLSNTPAEVAYHTTTVAN
                     DTVNVAYRLRFTFLSAAGTYTGKVIYTCTATF"
     gene            complement(6913..8046)
                     /locus_tag="VF272_04550"
     CDS             complement(6913..8046)
                     /locus_tag="VF272_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7260171.1"
                     /translation="MRNITFGTWREKRWVNVLIALSVGLLSLTSFTSAFAAGNLTNAS
                     ATLSDPRPEATAASVTYNISWKAATTATLQCFTYRWSTSSNPGQGSTPTGFSNAAATN
                     GTFTNLTAANWTIDNSTAGTVKNTYATGASVPANTQITTPIQSLTNPTFAAGGTYYIE
                     INTYSNAACSTLVDSTVIGFVTVPGVLVSAIVDSTLTFTVAGVASSTTYKGSLSTADR
                     CTDTATAVTFGSTTQPLAADTNYDCAQTLTTSTNANGGYQVTVQGKQTSGDFLHLLSD
                     PSQTVTNWTGTNGTPTATPTSGASEVFGYTTADSVLSGTPGRFTTTDNLFAGLTTTPA
                     EVAYNATAIANDATNVGYRLRITGLTEAGTYQGTLVYTCTPVF"
     gene            8150..8539
                     /locus_tag="VF272_04555"
     CDS             8150..8539
                     /locus_tag="VF272_04555"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HEX7260172.1"
                     /translation="MSDTAKRNALNRVYLKNHILRVDVIGDQTQETVEDMGRQLTDHI
                     VQLRKAGQKVLLLDNVQKIGRTDMQAVRAVAILGRILDYDRAAMVGDGSAPMRTGINL
                     LLRTIRRSSIQYFSNLKDAETWLREFN"
     gene            complement(8547..10295)
                     /gene="acsA"
                     /locus_tag="VF272_04560"
     CDS             complement(8547..10295)
                     /gene="acsA"
                     /locus_tag="VF272_04560"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX7260173.1"
                     /translation="MSEHPEVIQKKLTGQTPNLSDYEQAYQNFSWDEAANELIDLFPD
                     GALNIAYNCVDRHAEGARKAKTALLYESADGETQRFSFQDLKKLSNQFANVLAANDVA
                     KGDRVFIFLPPAPERYVAFLGTLKLGAVVGTMFAAFQEMALLDRLADSEAKVVVTDTD
                     LYPRIKNIQTKLPHLKKIIIVAGGSENSPEAGNVVLYEEAMAGAQDKFEVAHMQKDDY
                     AYMLYTSGTTGAPKGVVHAHYDILQAVVTTKYALDIQEEDVYWCTADLGWVTGVVYGV
                     LGIWGLGGTSVVYGGRFSPENWYNVLQSHKVTVWYSAPTAIRMLMGSSVSPKDYKLQS
                     LRHLCSVGEPLNPEAIWWGLDAFGLAFHDTWWQTELGGISITNIPALDIKPGSMGKPL
                     PGIEAAIIDDDGKPVPNGEEGNLALKASSVSSLMKTIWGNQEKFDSYFKGDWYISGDA
                     AMKDDDGYFWFFGRVDDVINTAGERIGPFEVESALVSYPDVIEAGVIGKPDQVRGEII
                     KAFVVVKSGVEANDELKERLRLHVKKHLAGHAYPREISFIDKLPKTRSGKIMRRILKA
                     QELGLPIGDTSTLENY"
     gene            complement(10292..13099)
                     /locus_tag="VF272_04565"
     CDS             complement(10292..13099)
                     /locus_tag="VF272_04565"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEX7260174.1"
                     /translation="MTENSASMHDIANIQKHIRAANYLSVAQIYLQDNCLLERPLRSS
                     DIKPKLFGHWGTCPGINFVYAHANHMVKKHEQEAVFVLGPGHGMPGLQANLFIEGTLA
                     KYYPEATHDGSGVNYISKMFSWPYGFSSHCSPETPGLILEGGELGYSLASAYGAVLDN
                     PDLLAVCLIGDGEAETGAIAAAWHTSKLIDPATNGAVLPIMHLNGYKISGPTIFGRMS
                     DEELQQLFSGYGYEPFIVEGSDDEIDERMMSTLETCYNRIAAIQKRARETGDDFAPRF
                     PMIIFKSPKGWTTIKELRGQPIEGTIRSHQVVMPTVAGDDEELRILESWLRSYRFEEL
                     FDKNTGFIEDIKEVVPPDNLRMGDNPHMFGANYKPLMLPDASKLQKPMVEPGMLQSNA
                     MRMAGSYLKELFADNKEHRNLRLMSPDETYSNRLDEIFEVTSRGFVWPHEKGDKDLSR
                     DGRVMEMLSEHTMHGLAQGYVLTGRHAVFTTYEAFAQIFSSMAHMYQKFLKYVRRMPW
                     RQDIPSINYLLTSTAWRQEHNGYSHQNPSFVSGMLEKHSDFVKAYFPVDDNSMLAVME
                     ETMASTNQMNIITAGKTPEPRWQTYAQAKEALKDGLSVWDFASDKNPHVVVVGIGDYV
                     TKEAMAAIERIKQDAPEIRIRFVNVLRLQGVCSCENESHPQIPSAEKYFTVDKPVILT
                     FHGYPETLKSILFDVKNPQRFTVHGYEEEGGTTTPFDMQVRNRTDRYHLAMELAEIGL
                     KEGAIDAATQQKLTKSYQKALDDHHTYILNVGGDPTEIENWQWSGTPPPIVASSAQER
                     IKALKEARTIAFIGLSDNPSRHSYKVAQHFQEKGYRIIPINPNIDETLGEKAYDSLLD
                     IPSSVHIDIVDIFRKPTEVIPHLKEIMDRGGIRTVWLAEGANSHEAEEFAEDYGLYLV
                     TNYCIMDVDIGGENAPEMVGV"
BASE COUNT         3270 a         3348 c         3163 g         3341 t
ORIGIN      
        1 cgctaatttt gtcacaccca ccgagctagc cgacggcgtg tatttcggat ttagcgatct
       61 tagcaactac cgccaccacc cctcaatcat ttttattggc attcccacta cgatgggaaa
      121 cactcagcgc cggattgaaa cctacttgct tgatatccca gataaagatt actaccaact
      181 tccggtaact attgtacttg agtattttca ccgcactaat caaaccttcg attctgttaa
      241 tgcgctgatg gcagccatga agaccgacga ggccacaatg cgcaagtggc tatcgaccct
      301 gtaatgcgtc catcataatc cctagggaaa ccaagacccg ccaatcggtt ttctgcagca
      361 actgatcatc ggcgcttaac cggtaatggt cacgcagggt tgtcaccttt tcgtaactcc
      421 cgtagaccgt ttggtcgctg gggttaataa gctcgaagtg atacttaata aaaaatggga
      481 tttcaccgat caacggcagt aactcccaca accggcggaa aatggccagc gcttgactgc
      541 gctcacgggc aataaccaag ggaatcgttt cgttatcggg cgtaaatacg tgccaggtac
      601 tacgcaggaa cgaagccccg aaggccttgc caataacacc caggaccgta ccctgtgcat
      661 cgcgcacgtc atatcgagca ccaaaatcca ttacctgtcg agcctgcacc tccatgagca
      721 tcttctgctt ggactcatcg gcgtaaaagg taaatttttc cttccaagca aagcgttttt
      781 gatgcgcaaa tgccagtaac ttcccttttt caccggaaac atccagctca tacacataat
      841 attgattggc cagcggtgca atcttctgat caatagcgaa tgatttcatc ggcatccgca
      901 tccactggtt ggttgaagaa tcatattccc tattgtagcg ccaaaaagaa aatttaccct
      961 aattactgcc cgttctctgt ttttggcggg aaccttaccg ccttcttccc tgagttggct
     1021 tagccccgca gcactagctt gcctgcggtt agcaaacatc ccctctgcca tagtgatcca
     1081 tattgctacc tccggttgct gcgaggacgg cgtcgttccg gcattcgctc gccaaatccc
     1141 atcgcatgct cgcgcctggt aatcggctgt gacggaaggt cctcgaactc atctggttcc
     1201 atagtcacaa ccatctcctt tgatccggcg gctaaggcat taaacagcag cgcatgaagg
     1261 tatccggcaa cgaagccaat tgcaaaataa aagagtggca ccaggagtaa ggtaagcaag
     1321 ctagcactca ggccaaaaat cagaccctgc aacaaactgt tcgtaagcgc agtttggagc
     1381 gcagcgctct caatgaaaac cgcaactgcc aagagtatac ctagagctaa gccaagcgtg
     1441 ccttcaaaaa ttgccagggc aagcggcctt atgccggtaa tacgtgtctg ttgatccata
     1501 cctcctcctt tcgcgctcta tgcaaacgct aatccttatt atctccatcc tactccgcat
     1561 gattgcttgg agcatcagtg attcttaccg aaatgctcgg ataaaaaagg cttatcttag
     1621 aaaaatggag aggctatttt ccggctgatt cctcttgctt gcgtcggcga agcggaccca
     1681 gccgcatgaa gccgcgctgc gctttaaggg tgccattcac cagccaacca aatggacgcc
     1741 aggcggctac ttcacgtaac gcttcatgca acggatcatg caatggagcg tcattaccac
     1801 cttttttgag aagatacgtg taatatctcc agaggctata aaatatgata tttaggatga
     1861 gtaagattat aatcaacagc cactgcagta ccgcaatgct aaggttgatg gattttccga
     1921 aagattgctg agcggctgtg gcagttatag tgacggtgcg aacctttgtt ggctcgcttt
     1981 cggcccctac actggtcgta cggctaaaga aaactttgta cgaaccgggg aacaattcgg
     2041 cattatacct atactcccag gtaatgccct ctggaaatcc accccctcct ttgctcttcg
     2101 actttgctac ctgcttggtt ttagcaataa actggtcgac caacttacca ttcaagtaca
     2161 caaaaatagt atcggtagca gatgattgcc caataaactt catcggtttt tgaataacca
     2221 atgaagttgg gccgcttaca atggttggcg gcgtgatgcc ttcaacctta aacgacaagg
     2281 aagcctcagt tgtattgccc gctctgtcat gagccgtcac aacaattgta tgctctcccg
     2341 gcttctgcct aggaacggca tcaccagaag ccatagggaa agccggaccg ccgtcaatac
     2401 tggcggtata tcgttccact cccgaggtgg catcgaccga attaaaattc attttaggag
     2461 taggatttga ggagttgcca ccattgtccg gaatgaccgc aaaatttccg ggcggtttgg
     2521 aatcaattct gattgcaaaa tgcaccgtgg cgctaccaga tgtgtttgaa cctttcaaat
     2581 ggaagtacca cagcccgtcc tccagcgttt tagtggtctg aatcgtggca ttattcggag
     2641 tggtcggatt cccggtcggg ctatgatcaa acttaaacga atagttgccg atggcggtag
     2701 tggcatacca gtttacattg acgtcatggg ccttgtacca ttgattttga ttcggatgag
     2761 tcttggagac aaccgacagt aacggttgag gcggagcttt agccgctacc gtaaaggtag
     2821 ttcccgatga gcagcagaga atattggtgc ccataccatc gttcgctaat atttggcttc
     2881 cggctacatg cacgctatag gtgccaggct gggttgcatt aaagataacc gttacaaccc
     2941 tgcctgcaga cccgttatac ccgggattgg acaagccccc ggaaaagtat accgaggaga
     3001 ccgagccaac cggaccggct cccccgccgc ttgtccaggt tgtaaagatt gagctagcgg
     3061 tagatacgaa tgaatactgg agacccggcg tccacgtaat ggtagcctca ccagcattaa
     3121 ttgcctgtcc ggccgaatta atcataattg caacccggat aacatcgccg acttgatagg
     3181 tagttgccgt cggggaggcg tagaagctgg ccccgccggc cgcttgtgtt ggttgcgtcg
     3241 atgatccgac tgccataccc aaagccgccg caatcaccac cataataccc ataaagcgtt
     3301 tcatggcatt tccaccgtta tcgggctcag ctccaatggt actgctgttc ctttgccgtc
     3361 attcaataat acttgggtac ccgaggcaaa cgctaaggtg gtcgtgagcg gccttttcga
     3421 acgcaaaatc accgctccaa cctgggcttt acctttaaaa cctgggctaa atacgccacc
     3481 ggcaaagctg atggtgccag cttcattaga aaactgaggc tgatcatcaa tccaaagcgt
     3541 aaagaacgac tgttccttgc ttacacgtac cacttcaacc aaagcattat caaacctaag
     3601 attaacttcg gccgcattaa tggttaggcc cccggtatca atttcaatcg gtaccttcag
     3661 ttgatggccg gtttgagcgg ttgtttccga tgcaccgatg cttgccttgc gaggcttggt
     3721 tgtttgcatg atgactgcta ggccaacaac aaatagcgcc actactagca gcaggccgat
     3781 gatacgtagt ttggctgttg atgccattat ttttgtttcg tttggattca taatcgtttg
     3841 ttttaaaaac gcttatgttt ataataccct atcctgtcca aaaaaacagc attattgaaa
     3901 aatcggttaa attagttttg gtatcatcat taatatcggc cggtttgttg atattgattt
     3961 gaaggtagaa aaacatcaga atcgaaaaat cggttaaatt gatctttgtg tcgttattga
     4021 gatcagccga gagcaccacc tgaaagcttg tgtcgggact ttgagggctt agtgcccctc
     4081 cgggagcttg aaccaccgcg ctggtcgtat aggtgcccaa atgcagggtc tgtgaaggag
     4141 atccgttcca gttcccactg gcagaggttg catcttgggt ggtaaaggag tcactgtatt
     4201 ttgtaccgct ccagagcgtt gttacagtag aattgtgccg cgctgttccg ctcgccactt
     4261 gagtttgcgg gcgcttgatt ggatttaatg ccaaactaaa ggtcgttggc agcaaatatc
     4321 cgctcagtgt ggtagtgaat gctggcaaaa tggttgcaat cgtagtaatt tctgcagtgg
     4381 tgcggctact caaatcagta gcgtaaatgc tatacgtagc gcttccgaga ggctgggtcg
     4441 taaaagtcat gtcgaaggag ctgtcgctgc ctgctacctg cgtaccgatc acgctgccgt
     4501 tacgctggaa cgttaccaac gcgctcggag aagcctctcc tacaaatcga atccctccgg
     4561 tttgaaccgg caactcggcg ccgacatcgg actcagagga ggcggtgaac gacgtatgag
     4621 accaccccga caagccaata aggctggcaa gcagcaggat gaaagccaca gctcgccgca
     4681 tggacactag cgccctctaa tcagtactcg agcagcattt agtaatcggt ggcgcaagac
     4741 aatcagcaac accagaacaa cgaggattat caggaatggg atcagcggga aagcccaaaa
     4801 cttagtttca gctgttagaa ttttattttc cgatccgtaa accgctgccg catgagcgct
     4861 gtaataaccg ccccatagat cggtttttgg ccaaatcacc gtaattcgac gctcgccacc
     4921 gggaaaaacg ttacgcggat taatcgctag ttgatcaacc tttttaccaa agagattata
     4981 gatggtaacg gtgcccgccg gcttaaaatg aacattaccg gaatttttaa ataaaatatc
     5041 gaaggcgatc ggccccttgc taaagaacga ctgcttatgc tcaccgcgta aatcttcagc
     5101 actggcaacc aaaattttgg ttttgccgtc ggaaccggtt atcgactgcc atgatccttt
     5161 gaatgtttta gtatgaaacg attcaatact cccctgttcc ttgatcgctc cgctcactcg
     5221 catcaatatc aatgaaccta cccgctgaat aacccgggca ccggttgagg tggcgtttgc
     5281 ggttaccacc ggattaaata gcacggtggc aaaatgaccg ccaggctcag cctgcttggg
     5341 cacggtaatc gtaaacggaa ccaacgccga tgcttttggc gctagctgca gctgggttgt
     5401 gggaaaccga aaccacttcg aaaatgccgc tggatcaacg ctatcgttta ataccgaaac
     5461 cgacccttcg gtgcctccaa ccgtaaaatc ttgagtactt acctggagcg ttacggcgct
     5521 atcggagtcg tttgtaattc gaatggtatt attcagcgaa tccccagggt tggcgctgag
     5581 ctcataggtc ggcggcgaca ccgttaaacc caggggggtg gcagcctgca catgattaag
     5641 cgttccgaag ctggcagcta tcgcaacacc aattgctaca cgcataaaat tttgaatcat
     5701 tgttccgtaa tggttatgct atccattcta gaaggttgcg gtacacgtgt aaataacttt
     5761 tccagtatac gtaccggcag ccgataaaaa ggtaaacctc agccgatacg ccacattcac
     5821 cgtatcattg gccactgtag tggtgtggta ggcgacttct gccggggtat tactaaggcc
     5881 agcgaacaaa ttatcactcg tgctaaaccg gtttgcgctg ccgcttaagc tcgagtcgct
     5941 cgtggtatag gcgaatattt ccgcagttcc tccgggagta gctgttgggc tggcgttggt
     6001 gccggtccag tcgggaagtg aagagggccc gtttttcaat gtgttcccag ggtcaatcgt
     6061 gtttaccgtt acttggtagc ctccgttccc attagtactg gtagtaaggg tttggccgca
     6121 atcatagttg gtgtcgggcg atagcggcaa taaaggcgtt ccaaagctaa tagaagtagc
     6181 actgtccaca cagcggtcgg atgtgttaag cgctccttta tagacagttg aagccggtat
     6241 tccgcttacg gaaaaattca gtgttggatc caccgtagcg ctaacggtga taccggaaac
     6301 gatcgtcaag gccacggtcc cattatcgac gtcagtagca taggttgcgt cagaatagct
     6361 agtaagccgc atgtaatacg taccttccga tgcgggattg gtgacattgc taaaaataat
     6421 ttgctgcgag ccgctgggag ctgccacgtt gccggcttta accatttgca gggagccggt
     6481 aaccgggtcg cttacccagc cgctgccatc gccaaaaccg gttggctggc tcgcaagcag
     6541 tgcgaatgag cctgaaaatc cggccggtgt agtgcaactg ccggagggag ttgtgcacat
     6601 ttgagcccgg aatgccttca ggaccgttgt gctggctacg ctgaaattaa aggtataggt
     6661 ggttccgttc tgagcggggc gaggatctga aagtaaaata gtggcgctgg ataaggatcc
     6721 ggcataggcg atcgtcgcca tccacagcac tttgctggca cccacagcga agaagagtgc
     6781 tatgacagca tgcaggcgtg ctgtaaatgt gtgacgtcga tttctcataa attgtttgtt
     6841 tttgtgttgg tttttagtta tctgtcacta caagagtacc agatggggga ctaggagtca
     6901 atgcgcttgt gcttagaata ccggagtgca ggtataaacc aatgttccct ggtaggtgcc
     6961 ggcttctgtt agcccggtga tacgcaggcg gtaacccacg ttggtggcat cgtttgcaat
     7021 tgcggtagca ttgtaagcaa cttctgcggg agttgtagta agaccggcaa aaagattgtc
     7081 ggtagttgtg aatcgtccag gagtacccga tagcacgcta tcggcagtag tgtagccaaa
     7141 cacttcgctt gcaccactag tcggtgtagc ggtcggagta ccgttggtgc cggtccagtt
     7201 ggtgacagtt tgtgatgggt cgctgagcaa gtgcaggaag tctcccgatg tctgcttgcc
     7261 ttgcactgtc acctgatagc ctccgttggc gtttgtactg gtagtcaatg tttgtgcaca
     7321 atcataattg gtatcagcgg ctaaaggctg ggtggtgctg ccgaaggtaa cggcggtagc
     7381 ggtgtcagta cagcggtcgg cagtactcaa tgagccctta tacgtggttg atgacgccac
     7441 acccgccacg gtaaaggtaa gggttgaatc aacaattgcg cttaccaata cgcccgggac
     7501 ggtgacgaag ccaattaccg tactgtcaac caaggttgag caggcagcgt tgctgtaggt
     7561 attaatttca atgtagtagg ttccgccggc ggcaaaggtc gggttggtca gcgattgaat
     7621 cggtgtggta atctgagtgt tcgccggtac cgacgcaccc gtagcataag tgttctttac
     7681 cgtgcccgcc gttgagttat cgatggtcca gtttgcagcg gttaagttag taaaggtacc
     7741 attggtcgct gctgcattag aaaacccggt tggcgttgaa ccttggccgg ggttggatga
     7801 ggtgctccac cgataggtaa aacactgcaa tgtagcagtt gtagcagctt tccagctaat
     7861 attataggta acactggcgg cagtggcttc aggccttgga tcgctcagcg ttgcgctcgc
     7921 attggttaag ttaccggctg caaacgccga tgtaaagcta gttaaactta gtagtcctac
     7981 cgacagcgct atgaggacgt taacccagcg cttctcgcgc cacgtaccaa atgttatgtt
     8041 cctcactgta aactcctact tttttatttg ttagcctaag cataaccgca atggaaaact
     8101 tggtcaagct ttttttcgcg cattttgtca atctgatatg gtggtatata tgtcagacac
     8161 cgccaaacgc aatgcgctta accgcgtcta tcttaaaaac cacattttac gagtcgatgt
     8221 tattggggat caaacccaag agacggtcga ggatatgggt cggcagctta ccgatcatat
     8281 tgttcaactg cgcaaagccg gacaaaaggt tctcctatta gacaacgttc agaagatagg
     8341 taggaccgat atgcaagccg tgcgggcggt cgccattctt ggacgcatac tcgattatga
     8401 tcgcgccgcc atggttggcg acggctcggc gcccatgcgc accggtatta atttgctgct
     8461 tcgtacaatt cgacgatcaa gcatacagta cttttctaac ctaaaagatg ctgaaacctg
     8521 gctgcgcgaa ttcaattagc tgcttgctaa tagttctcca gcgttgaggt atcgccaatt
     8581 ggcaagccaa gttcttgggc tttgagaatt cggcgcataa ttttaccgga acgagttttg
     8641 gggagtttat caataaaact aatttctctt gggtaggcat gtccggccag atgtttttta
     8701 acatgcagtc gtaacctttc tttcagttca tcattagctt ccacgccaga ctttaccact
     8761 acaaaagcct taataatttc gccccgcact tgatccggct tgccaatcac gccagcttct
     8821 atgacatccg gatagctcac caatgcactt tccacctcaa acggaccgat gcgttctccg
     8881 gcggtattaa tgacgtcatc aactcgccca aaaaaccaaa aatagccgtc atcgtccttc
     8941 attgctgcat cgccggatat gtaccagtcg cccttaaagt aggagtcaaa tttttcttga
     9001 tttccccaaa tggttttcat cagcgaggaa acggacgaag ctttgagcgc cagattccct
     9061 tcctcgccat tgggcaccgg cttgccgtca tcgtcaataa tagcggcctc tatgcccgga
     9121 agcggcttac ccatcgaacc cggtttaata tcaagcgctg ggatgttggt gatgctgatg
     9181 ccgcccagct cggtctgcca ccaggtatca tgaaaggcta gcccgaaagc atcgagcccc
     9241 caccaaattg cttcggggtt cagtggctct ccaacactgc ataaatgccg aagtgattgc
     9301 agtttataat ccttagggct aaccgaagat cccataagca tgcgaatggc ggttggagcc
     9361 gagtaccaca ccgttacttt gtgcgactgt aaaacgttat accaattctc gggactaaat
     9421 ctcccaccat acaccaccga ggtaccgcca agcccccata tgcctagcac gccatataca
     9481 acgccggtca cccagcctaa atcggccgtg caccaatata catcttcctc ttgaatatcg
     9541 agcgcatact tcgtggtaac gaccgcttga agaatatcgt aatgcgcatg caccactccc
     9601 ttcggcgcac ctgtagttcc agaggtgtaa agcatgtagg catagtcgtc tttttgcata
     9661 tgagccacct caaacttgtc ttgagcgccc gccatcgctt cctcatagag cacgacgttg
     9721 ccggcttcgg gactattctc cgagccgcct gcgacaataa taattttttt caaatgcggc
     9781 agtttagttt gaatgttctt gatgcgagga taaagatcgg tatcagtcac aaccaccttg
     9841 gcttcgctat cggccaaacg gtcaagtagt gccatttcct ggaaggctgc aaacatcgtc
     9901 ccgacaaccg ccccaagttt aagggtacct aaaaaggcca cgtacctttc aggtgcgggg
     9961 ggtagaaaaa taaacacccg atcccctttt gcaacatcat tcgctgccaa aacgttggca
    10021 aactggttgc tcagtttctt taaatcttga aagctaaatc tctgcgtttc cccgtctgca
    10081 ctttcataaa gcaaggctgt cttggctttg cgagcgccct cggcgtggcg gtcaacgcag
    10141 ttataagcaa tattgagggc tccatcgggg aacagatcta tgagctcgtt tgcggcttca
    10201 tcccatgaaa aattttggta agcctgctcg tagtcggaca agttgggcgt ttggccagtt
    10261 aatttctttt gaattacttc cggatgttcg ctcatacacc taccatctcc ggagcatttt
    10321 ccccgccaat atcgacatcc ataatacaat aattggtaac caggtacagg ccgtaatctt
    10381 cagcaaattc ctcggcttca tggctgttcg caccttcagc cagccacacc gttctaatgc
    10441 cccctcgatc cataatttcc ttcaaatgcg ggatcacttc ggtaggcttg cgaaaaatat
    10501 cgacaatatc aatatgcact gaggacggta tgtcgagcaa gctgtcatag gctttctctc
    10561 caagcgtctc gtcgatattg gggttgatcg gaataatccg ataccccttc tcttggaagt
    10621 gctgagctac tttgtaacta tgtcggctag ggttatcgga caagccaata aaagcaattg
    10681 tgcgggcctc tttaagagcc ttaattcttt cctgagcgga gctagcaaca atcggcggcg
    10741 gggtacccga ccactgccag ttttcgattt cagtagggtc gccgccaacg ttaagaatat
    10801 aggtgtgatg atcatcgagc gccttttgat agcttttagt cagcttctgc tgggtagccg
    10861 catcaatagc accttctttg aggccaattt ccgctagttc catggcaagg tgataccggt
    10921 cggtccggtt tctaacctgc atatcgaacg gcgtggtagt acccccttct tcctcatacc
    10981 catgaaccgt aaagcgctgg ggatttttta catcaaaaag aatactctta agcgtttccg
    11041 gatagccgtg aaaggttaaa ataaccggct tatcgacagt gaagtatttt tcagccgagg
    11101 gaatttgcgg gtggctttca ttctcgcaag agcagacacc ttgcaaccgc agaacattca
    11161 cgaaccgaat cctgatctcc ggagcatcct gttttatacg ttctatggcg gccatcgctt
    11221 ctttcgtcac gtaatcgcca ataccaacta ccacaacatg aggattttta tcgctggcaa
    11281 aatcccacac gctcaaacca tccttcaaag cctccttggc ttgagcatag gtttgccacc
    11341 gaggctcggg cgtcttacca gccgtaataa tattcatctg gttggttgag gccatggttt
    11401 cttccataac cgccagcata ctgttgtcat ccaccggaaa atacgccttc acaaaatcgc
    11461 tgtgcttttc gagcatgcct gaaacaaaac tgggattctg gtgggaatag ccgttatgct
    11521 cctgacgcca ggctgtcgaa gtcagcaaat aattaatcga tggtatatct tgacgccatg
    11581 gcatgcgacg gacatatttc agaaactttt gatacatgtg ggccatactt gaaaaaatct
    11641 gtgcaaaagc ttcataggtt gtgaagacgg cgtggcggcc agtgaggaca taaccttgag
    11701 ccaaaccgtg catggtatgc tccgacaaca tctccatgac tctcccgtcc cggctcaaat
    11761 ccttatcgcc tttttcgtgc ggccaaacaa acccccgcga tgtaacttca aaaatctcat
    11821 ccaatcggtt ggaataggtt tcgtcgggag acatcaagcg cagattacga tgctccttat
    11881 tatcggcaaa cagctccttc aggtagctgc cggccatccg catagcattt gactgtagca
    11941 tccccggttc aaccatcggc ttttgcagtt ttgaagcgtc gggcaacatc aatggcttat
    12001 aattggcgcc gaacatatgc gggttatcac ccatgcgtaa attatccgga ggaacaactt
    12061 ctttaatatc ctcaataaac ccagtgtttt tatcaaacag ctcctcaaac cggtacgagc
    12121 gcagccatga ttccagaatg cgcagctcct cgtcgtcgcc ggccaccgtt ggcattacca
    12181 cttgatggga gcgaatggtg ccttcaatcg gttggccgcg caattcctta atcgtagtcc
    12241 aacccttcgg acttttgaag ataatcattg ggaagcgcgg tgcgaagtca tcgccggttt
    12301 cacgcgcccg tttttgaata gccgcaatcc ggttataaca ggtttcgagc gtggacatca
    12361 tgcgttcatc gatctcgtca tcgctgcctt caacaataaa cggctcatac ccgtaaccgc
    12421 taaagagctg ttgcagctcc tcatcgctca tccggccgaa aatggttgga ccagaaatct
    12481 tatacccgtt caaatgcata atcggcagta ccgcaccgtt agtggccgga tcaatcagtt
    12541 tgctcgtatg ccaagcagca gcaatcgcgc cggtctcagc ttcgccgtca ccaatcaaac
    12601 aaacagccag gaggtcagga ttgtctagta ctgcaccata ggcggaagcc agcgagtatc
    12661 ccaactcgcc accctctaag attaatccgg gcgtttcagg actgcagtgg ctggaaaaac
    12721 catacggcca gctaaacatc ttgctgatgt agttcacccc ggaaccatca tgggttgctt
    12781 ccgggtaata ctttgcaagc gtaccttcaa taaataaatt ggcttgcaga ccgggcattc
    12841 cgtggccggg accgagtaca aacaccgcct cctgctcatg cttttttacc atatggttgg
    12901 catgagcata cacaaaattg ataccgggac aggtacccca atgaccaaac agttttggct
    12961 tgatatcgga tgaacgcaat ggacgctcaa gcagacagtt atcctgcaag taaatttgag
    13021 caaccgataa atagttggcc gccctaatgt gtttttgaat gttggcaata tcgtgcatag
    13081 atgcggaatt ctctgtcatg ttacctgatg tgatttagct cg
//
