LOCUS       DASVII010000001         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_359593,
            whole genome shotgun sequence.
ACCESSION   DASVII010000001 DASVII010000000
VERSION     DASVII010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359593"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..456)
                     /locus_tag="VF309_00005"
     CDS             complement(40..456)
                     /locus_tag="VF309_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7464989.1"
                     /translation="MNVRDVMTRNPRTVAPNDSIQRAAAVMKEVDTGFVPIVENGKVV
                     GVVTDRDIVIRAVAAGSYDKPVSSIATRELVFVAPGDSLRTAEKLMSERQVRRIAVIE
                     DDLLVGIVSLGDIAVKEGKDSRSGSVLENISEGVKH"
     gene            807..>1576
                     /locus_tag="VF309_00010"
     CDS             807..>1576
                     /locus_tag="VF309_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7464990.1"
                     /translation="MSNLHPNRWLTGLWLNRDFRKLWGSLTIVHFGGQVTFLALPLTA
                     ALVLNASPFEVGVLTALEALPYPIFGLFAGVLVDRARKLPVIIACDVGRALALLAIPL
                     AAWFGVLSMAILYVSGFLMGLFTVIGWPAYQVFMTERVGREHLVEANAKIGVADSAAQ
                     LVGPGLAGALIQWLTAPFAILLDAMCFFFSAWMLRGIPPAASDTPKGTPRSVRSEIAE
                     GLRVIWRNPTLRALVWGIGTWQVFRHAFIAIVVLFGAR"
BASE COUNT          223 a          519 c          530 g          304 t
ORIGIN      
        1 cagaacaggg tcagagcggg gtctgacccc ggtttgggtt cagtgcttga cgccctcgga
       61 gatgttctcg aggacgctgc cgctgcggct gtcctttccc tctttcaccg cgatgtcgcc
      121 gagggagacg atgccgacca ggaggtcgtc ctcgatcacc gcgatgcgcc ggacctggcg
      181 ctcgctcatg agcttttcgg cggtgcgcag cgaatcgccg ggcgcgacga acacgagctc
      241 gcgcgtggcg atggagctca ccggcttgtc gtagctgccg gcggccacgg cgcggatcac
      301 gatgtcgcgg tcggtgacca cgcccaccac cttgccgttt tccacgatcg gaacgaagcc
      361 ggtgtcgacc tccttcatca cggcggcggc gcgttggatg ctgtcgttgg gcgcgacggt
      421 gcgcgggttg cgggtcatga catcgcggac attcatgggc ggactcctcg tgggtgggcg
      481 gtatgaccgc ggcggcgaat tttcgttttg ctataattcc cccctgcccc actttttccg
      541 gacccagacc gatgagacgc gacgcgaaaa ccttcgattc ccgagagatt tcctcggcgg
      601 tcgtgcgcgc gcgctccgga gccttgccgc tgtcgggcgg cgacgactga tcctgcgggc
      661 cgaccgtccg ccggattcaa caggcggaaa gagtcggcaa acaaaccgtt ccttttccgc
      721 cagcgcttca accgcgcgac ggcgaacccg ttgcgcaatg gaaaaaggat cccggatcag
      781 cgggacaaaa agacatggca acgatattga gcaatctgca tcccaaccgc tggctcaccg
      841 gcctgtggct gaaccgcgac ttccgcaagc tgtggggctc gctcacgatc gtccacttcg
      901 gcgggcaggt cacgttcctc gcattgccgc tcaccgcggc gctggtgctg aacgcctcgc
      961 ccttcgaggt cggcgtgctc accgcgctcg aggcgctccc ctatccgatc ttcggcctgt
     1021 tcgccggcgt gctggtggat cgcgcgcgca agctcccggt gatcatcgcc tgcgacgtgg
     1081 ggcgcgcatt ggcgctcctc gcgattccgc tcgccgcgtg gttcggggtg ctcagcatgg
     1141 cgatcctgta cgtgtcgggc ttcctcatgg ggctcttcac cgtgatcggc tggcccgcct
     1201 accaggtgtt catgaccgag cgcgtgggac gcgagcatct ggtcgaggcc aacgcgaaga
     1261 tcggggtggc ggattccgcg gcgcagctgg tcgggcccgg cctggccggc gccctcatcc
     1321 agtggctcac ggcgcccttt gccatcctgc tcgacgcgat gtgcttcttc ttctcggcgt
     1381 ggatgctgcg cggcattccg ccggcggcga gcgacacgcc caagggcacg ccgcgctcgg
     1441 tgcgctcgga gatcgccgaa gggttgcgcg tcatctggag gaatcccacg ctgcgcgcgc
     1501 tggtgtgggg gatcggcacg tggcaggtgt tccgtcatgc gttcatcgcg atcgtggtgc
     1561 tcttcggcgc gcgcga
//
LOCUS       DASVII010000002         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_287870,
            whole genome shotgun sequence.
ACCESSION   DASVII010000002 DASVII010000000
VERSION     DASVII010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287870"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF309_00015"
     CDS             <1..332
                     /locus_tag="VF309_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7464991.1"
                     /translation="AADPNKKWTLDELKSAGEKLYAANCSACHQPTGKGMPPAFPALD
                     GSKVATGPKAAHLATVLKGRPGTAMAPFAHLADADLAAIITYERNAWGNHTGEAIQPA
                     EIAAARK"
     gene            357..1973
                     /gene="ctaD"
                     /locus_tag="VF309_00020"
     CDS             357..1973
                     /gene="ctaD"
                     /locus_tag="VF309_00020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX7464992.1"
                     /translation="MSTASHTHVPGADDGHGHDDAHDHHGPPPGFTRWMYTTNHKDIG
                     TMYLWFAFFMFLLGGTMALAIRAELYKPGLQFLDPEFFNSLTTIHGLVMVFGAIMPAF
                     VGFANWLVPLQVGSADMAFPRMNNWSFWLLIPAALLLIGSFFVPGGAAASGWTLYPPL
                     VIQQGISVDMTILAVHILGASSIMGSINIITTILNMRAPGMTLMKMPLFVWTWLITAY
                     LLIAVMPVLAGAVTMLLTDRHFGTSFFNAAGGGDPTMFLHIFWFFGHPEVYIMILPAF
                     GIISQILPAFARKPLFGYASMVYATASIAILSFIVWGHHMFTVGMPAAGNLFFMYATM
                     LIAVPTGVKVFNWIATMWKGEMTFETPMLFAIGFLCLFTIGGFSGLVLAIVPVDITLH
                     NTYYVVAHFHYVLVSGALFAIIAGIYFWLPKWSGKMYNEGLGKLHFWLSTIFVNVLFF
                     PQHFLGLAGMPRRIPDYSTQFSEFNMLSSIGAFIFGFSQLIFLYNVIATIRSGKPAEQ
                     KQWEGADSLEWTHLPTPMPYHTFVTPPVVK"
     gene            2123..>2369
                     /locus_tag="VF309_00025"
     CDS             2123..>2369
                     /locus_tag="VF309_00025"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7464993.1"
                     /translation="MNAVVQLENRRLLGKLVVIAVGMLGFGFAMVPMYRQICQAIGLD
                     STRVVAAATNTQVDASREVTVEFLASSAGLPWRFEALD"
BASE COUNT          391 a          827 c          708 g          443 t
ORIGIN      
        1 cagccgccga ccccaacaag aagtggacgc tcgacgagct gaagtcggcc ggcgagaagc
       61 tctatgcggc gaattgctcg gcgtgccacc agccgaccgg caagggcatg ccgcccgcat
      121 tcccggccct cgacggctcg aaggtcgcga cggggccgaa ggccgcgcat ctcgcgacgg
      181 tgctcaaggg ccggccggga acggcgatgg ccccgttcgc gcacctcgcc gatgccgacc
      241 tcgcggcaat catcacgtac gagcgcaacg cctggggcaa ccacacgggc gaagcgatcc
      301 agccggccga gatcgcggcc gcgcgcaagt aacgaattcc agaaagcgga gcacacatga
      361 gcacagcgag ccacacccac gttcccgggg ctgacgacgg ccacggacac gatgacgccc
      421 acgaccatca cggcccgccc cccggcttca cacgctggat gtacacgacc aaccacaagg
      481 acatcggcac gatgtacctc tggttcgcgt tcttcatgtt cctcctcggc ggcaccatgg
      541 cgctcgccat ccgcgcggag ctctacaagc cggggctgca gttcctcgat cccgagttct
      601 tcaactcgct caccacgatc cacgggctgg tgatggtgtt cggcgcgatc atgccggcct
      661 tcgtgggatt cgcgaactgg ctggtgccgc tgcaggtcgg ctccgcggac atggcgttcc
      721 cgcgcatgaa caactggagc ttctggctgc tgattcccgc ggcgctgctc ctgatcggct
      781 cgttcttcgt tcccggcggc gccgccgcct cgggctggac gctctatccc ccgctcgtga
      841 tccagcaggg gatttccgtc gacatgacca tcctcgcggt ccacatcctc ggcgcctcgt
      901 cgatcatggg ttcgatcaac atcatcacga ccatcctcaa catgcgcgcg ccgggcatga
      961 cgctcatgaa gatgcccctc ttcgtgtgga cctggctcat caccgcctac ctgctgatcg
     1021 cggtgatgcc ggtgctcgcg ggcgcggtga cgatgctcct caccgaccgc cacttcggca
     1081 cgagcttctt caacgcggcg ggcggcggcg acccgacgat gttcctgcac atcttctggt
     1141 tcttcggcca tcccgaggtg tacatcatga tcctgccggc cttcgggatc atctcgcaga
     1201 tcctgccggc cttcgcgcgc aagccgctct tcggctacgc gtcgatggtc tacgcgacgg
     1261 cctcgatcgc gatcctgtcg ttcatcgtct ggggccacca catgttcacc gtcggcatgc
     1321 cggccgcggg caacctcttc ttcatgtacg cgacgatgct gatcgccgtg cccacgggcg
     1381 tgaaggtctt caactggatc gcgacgatgt ggaagggcga gatgaccttc gagacgccga
     1441 tgctgttcgc gatcgggttc ctgtgcctct tcaccatcgg cggattctcc gggctcgtgc
     1501 tcgcgatcgt tcccgtcgac atcacgttgc acaacacgta ctacgtcgtc gcgcacttcc
     1561 actacgtgct cgtgtcgggc gcgctcttcg cgatcatcgc cggcatctat ttctggctgc
     1621 cgaagtggtc gggcaagatg tacaacgagg gcctcggcaa gctccacttc tggctgtcga
     1681 cgatcttcgt caacgtgctg ttcttcccgc agcatttcct cggcctggcc ggcatgccgc
     1741 gccgcatccc cgactactcc acccagttct ccgaattcaa catgctctcc tcgatcggcg
     1801 ccttcatctt cggcttctcg cagctgatct tcctctacaa cgtgatcgcg acgatccgct
     1861 ccggcaagcc cgccgagcag aagcagtggg agggcgcgga ctcgctcgag tggacgcacc
     1921 tgccgacgcc gatgccgtac cacacgttcg tgacgccgcc ggtcgtcaag taggcgcgag
     1981 gcccaatgag attcgaggca cgaggcacga ggcgcgagat gacccccaag cagcgcaagg
     2041 gcgccgtcat ctccgcgatc actctcgccg cgatggcgat cgggatctac ctcgtcgtca
     2101 tgatgaagtt cttcgtctac aagtgaacgc cgtcgtacag ctcgagaatc gccgcctgtt
     2161 gggcaagctc gtcgtgatcg cggtcggaat gctcggcttc ggattcgcga tggtgccgat
     2221 gtaccgccag atctgccagg cgatcggcct cgactcgacg agggtcgtcg ccgcggcaac
     2281 gaacacccag gtggacgcga gccgcgaggt gacggtcgag ttcctcgcct cgagcgcggg
     2341 gttgccgtgg cgcttcgaag cgctcgacc
//
LOCUS       DASVII010000003         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_467715,
            whole genome shotgun sequence.
ACCESSION   DASVII010000003 DASVII010000000
VERSION     DASVII010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467715"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VF309_00030"
     CDS             <1..649
                     /locus_tag="VF309_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7464994.1"
                     /translation="LEAEAILLAESDGTREEVAHEVAEMIEVMRASGATQIRCSSSDA
                     ERLRFWSGRKAAFPAVGRISPDYYCIDGSIPRGALAKVLKRTGELSARYGLRCANVFH
                     AGDGNLHPLILYDANIPGELETTERFASDILELCIEVGGTITGEHGVGIEKINQMCTQ
                     FRPAELVQFHAIKAAFDPEGLLNPGKGVPTLHRCAEYGAMRVKGGALPHPDLPRF"
     gene            649..2136
                     /locus_tag="VF309_00035"
     CDS             649..2136
                     /locus_tag="VF309_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7464995.1"
                     /translation="MNARALESAQPHLRARMLAALRGIRPPLLVIDDDEGLKPFETDA
                     FIAHRETPMLAVLPDSEDQVRAVVKACRALGVPVVSRGAGTGISGGAIPRNDGVLLVL
                     SKMKRVIAVDPLARSATVEPGVRNLQVSETVACHGLFYAPDPSSQSICSIGGNVAENS
                     GGVHCLKYGLTTHNLLAVRAIDADGDVVDAGSLALDAPGYDLLALLTGSEGNLGVITR
                     VTLRLVPLPERTETLLAAFASVRQAAEAVGEIIAAGIIPAALEMMDRIVIEACERVMA
                     MGFPVGAEALLLAEVDGSREEALESLQAVRVILERCGATQIRVAANDAERERLWKSRK
                     GAFTALATILPDYYTIDGTIPRRCLPEVLDRVYALAAEYGLIVGNVFHAGDGNIHPCI
                     FYDANRPGELERAEELGGRILEFCVEVGGTITGEHGVGIEKLRQMCVQFRAPELCAFH
                     AVKAAFDPDGILNPGKAVPTLKRCAEWGGTHMRAGRMPHADIPRF"
BASE COUNT          329 a          758 c          758 g          330 t
ORIGIN      
        1 cctcgaggcg gaggcgatcc tgctcgccga gtccgacggc acgcgcgaag aggtggcgca
       61 cgaggtggcg gagatgatcg aggtgatgcg cgcgagcggg gcgacccaga tccgctgctc
      121 gtcgagcgac gccgagcgcc tgcgcttctg gtcggggcgc aaggccgcgt ttccggcggt
      181 cggccgcatc tcgccggact actattgcat cgacggatcg attccgcgcg gcgcgctcgc
      241 gaaggtcctg aagcgcaccg gggagctttc ggcgcgctac gggttgcgct gcgccaacgt
      301 cttccacgcg ggcgacggca acctccatcc gctcatcctc tacgacgcga acatccccgg
      361 ggagctggaa acgacggagc gcttcgcttc cgacatcctc gagctgtgca tcgaggtggg
      421 cggaaccatc acgggcgagc acggcgtggg catcgagaag atcaaccaga tgtgcaccca
      481 gtttcgcccc gccgagctcg tgcagttcca cgcgatcaag gcggcgttcg atcccgaggg
      541 cctgctcaat cccggcaagg gcgtgccgac tctgcaccgc tgcgcggaat acggcgcgat
      601 gcgcgtgaag ggcggggcgc tgccgcaccc ggacctgccg cgcttctgat gaacgcccgt
      661 gccctcgaat ccgcacagcc ccacctccgc gcgcggatgc tcgcggcgct tcgtgggatc
      721 cgcccgccgc ttctcgtcat cgacgacgac gagggcctga agccgttcga gaccgacgcc
      781 ttcatcgcgc accgcgagac gccgatgctc gcggtgctgc ccgacagcga agaccaggtg
      841 cgggccgtcg tcaaggcgtg ccgcgcgctc ggcgtcccgg tcgtgtcgcg gggcgccgga
      901 accgggatct ccggcggggc gattccgcgc aacgacgggg tcctgctggt gctgtcgaag
      961 atgaagcgcg tgatcgcggt cgacccgctc gcgcgcagcg ccaccgtcga gccgggggtg
     1021 cgcaacctgc aggtctcgga gaccgtcgcc tgccatgggc tcttctacgc gcccgatcct
     1081 tcgagccagt ccatctgctc catcggcggg aacgtcgccg agaattccgg gggcgtgcat
     1141 tgcctcaagt acgggctcac gacgcacaac ctcctcgcgg ttcgcgcgat cgacgccgac
     1201 ggcgatgtcg tcgatgccgg aagcctcgcg ctcgatgcgc cgggctacga cctcctcgcg
     1261 ctcctgacgg gcagcgaggg gaacctgggc gtcatcacgc gcgtgacgct gcgcctcgtt
     1321 ccattgcccg agcgcaccga gacgctgctc gccgcattcg cgagcgtgcg gcaggcggcc
     1381 gaagccgtgg gcgagatcat cgccgcgggc atcattcccg ccgcgctcga gatgatggat
     1441 cgcatcgtga tcgaggcgtg cgagcgcgtc atggcgatgg gcttccccgt gggggcggag
     1501 gcgctgctcc tcgcggaggt cgacggctcg agggaagagg cgctcgagtc gctgcaggcg
     1561 gtgcgcgtca tcctcgagcg ctgcggcgcg acgcagatcc gcgtcgccgc gaacgacgcg
     1621 gagcgcgagc gcctctggaa gtcgcgcaag ggcgccttca ccgcgctcgc gacgatcctg
     1681 cccgactact acaccatcga cggcaccatt ccgcggcgct gcctgccgga ggtgctcgac
     1741 cgcgtctatg cgctcgccgc cgaatacggc ttgatcgtgg gcaacgtctt ccacgcgggc
     1801 gacggcaaca tacatccgtg catcttctac gacgcgaacc ggcccggaga gctcgagagg
     1861 gcggaggagc tcggcgggcg catcctcgag ttctgcgtcg aggtgggcgg cacgatcacc
     1921 ggcgagcatg gcgtcggcat cgagaagctt cgccagatgt gcgtgcagtt ccgcgcgccc
     1981 gagctctgcg ccttccacgc ggtgaaggcc gcgttcgatc ccgacggcat cctcaacccc
     2041 ggcaaggcgg tgccgacgct caagcgttgc gccgaatggg gcggcacgca catgcgcgcg
     2101 ggccgcatgc cccacgccga cattccgaga ttctgatgag cgagcccttc atcgtcgcgc
     2161 tgtgcgagcg cattc
//
LOCUS       DASVII010000004         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_144106,
            whole genome shotgun sequence.
ACCESSION   DASVII010000004 DASVII010000000
VERSION     DASVII010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144106"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..821
                     /gene="hisF"
                     /locus_tag="VF309_00040"
     CDS             60..821
                     /gene="hisF"
                     /locus_tag="VF309_00040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7464996.1"
                     /translation="MSLAKRIIPCLDVHAGRVVKGVNFVALRDAGDPVETARTYDAAG
                     ADEIAFLDITASSDARDTIIDVIERVADEVFIPLTVGGGVRSVEDVRRLLNAGADKVS
                     INTAAVDDPDLVTRAASRFGAQAIVVAIDARAAGAGRWEVFTHGGRKARGLDVVEWAR
                     EMARRGAGEILLTSMDRDGTKAGFDLELTRSVSEAVTVPVIASGGVGTLEHLALGIEQ
                     GRADAVLAASVFHFGEITIAQAKQFLAARGIEMRL"
     gene            818..1222
                     /gene="hisI"
                     /locus_tag="VF309_00045"
     CDS             818..1222
                     /gene="hisI"
                     /locus_tag="VF309_00045"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7464997.1"
                     /translation="MNANDWIEQVAWNADGLVPAVAQDATSGEVLMVAWMNRDSLERT
                     VESGEAHYWSRSRRSLWRKGESSGHTQRVLEIRLDCDADTVLLRVESVAGIACHTGRR
                     RCFFSVLESGPAGRRWVETDPVIGAGQAKSDG"
     gene            1215..1547
                     /locus_tag="VF309_00050"
     CDS             1215..1547
                     /locus_tag="VF309_00050"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX7464998.1"
                     /translation="MAERGILERLEAAIAARRGADPSASYVASLNAKGLDHILEKVAE
                     EAVETVIASKTGERAAIVHETADLWFHCLVMLAWHGIPAAEVLAELERREGRSGIDEK
                     ASRTKGAS"
     gene            1547..1906
                     /locus_tag="VF309_00055"
     CDS             1547..1906
                     /locus_tag="VF309_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX7464999.1"
                     /translation="MAAKSDCIFCKIVAGAIPSRKVYEDDDMLAFHDINPVAPVHFMM
                     IPKDHVDSLATVTDAHAAVLGKMMAKAGRLAREQGATDGFRTIVNTGRIGRQDVYHLH
                     IHIIGGPDPLPRMIAKD"
     gene            1921..2139
                     /gene="tatA"
                     /locus_tag="VF309_00060"
     CDS             1921..2139
                     /gene="tatA"
                     /locus_tag="VF309_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX7465000.1"
                     /translation="MGSLSIWHLLILLLVVLLIFGTKKLRNMGADIGGAVKGFKDGMK
                     GEEERKPTGEIASSASTIEGEIKDRQKS"
BASE COUNT          369 a          712 c          777 g          320 t
ORIGIN      
        1 gccatctacg agggaaagct cgatttcgcc gcggcggtga agctcgcttc cggcggcgcg
       61 tgagcctcgc caagcgcatc atcccgtgcc tcgacgtgca cgcggggcgc gtcgtgaagg
      121 gcgtgaactt cgtggccctg cgcgacgccg gcgatccggt ggagaccgcg cgcacctacg
      181 atgccgcggg cgccgacgag atcgcgttcc tcgacatcac cgcttcgagc gacgcccgcg
      241 acaccatcat cgacgtgatc gagcgcgtcg ccgacgaggt cttcattccc ctcaccgtcg
      301 gcggcggcgt gcgctcggtg gaggatgtgc gccggctgct caacgcgggc gcggacaagg
      361 tgagcatcaa caccgccgcc gtggacgacc cggacctcgt gacgcgcgcc gcatcgcggt
      421 tcggcgcgca ggccatcgtc gtcgccatcg acgcgcgcgc cgcgggcgcg ggccggtggg
      481 aggtcttcac ccacggcggg cgcaaggcgc gcggactcga tgtcgtagaa tgggcgcgcg
      541 aaatggcgcg gcgcggcgcg ggtgaaatcc tcctcacgag catggatcgc gacggaacga
      601 aggcgggctt cgacctcgag ctcacgcgct cggtgtccga ggcggtgacg gtgccggtga
      661 tcgcgagcgg cggcgtggga acgctcgagc atctcgcgct gggcatcgag caggggcgcg
      721 cggacgccgt cctcgcggcc tcggtgttcc acttcggcga gatcaccatc gcgcaggcga
      781 agcagttcct cgccgcgcgc ggaatcgaga tgcgattgtg aatgcgaacg actggatcga
      841 gcaggtggcg tggaacgccg acggcctggt tcccgccgtg gcgcaggacg cgacatcggg
      901 cgaggtgctg atggtcgcgt ggatgaatcg cgattcgctc gagcgcaccg tcgagtcggg
      961 cgaggcgcac tactggtcgc gctcgcgccg ctcgctgtgg agaaagggcg agagctcggg
     1021 acacacgcag cgggtcctgg agattcgcct cgattgcgac gcggacacgg tgctgctacg
     1081 cgtggagtcc gtggcgggca tcgcgtgcca cacggggcgc aggcgctgct tcttcagcgt
     1141 gctcgaatcg gggccggcgg ggcgccgctg ggtcgagacc gatccggtga tcggcgcggg
     1201 gcaggcgaaa tccgatggct gagcgcggga tcctcgagcg cctcgaggcg gcgatcgccg
     1261 cgcgccgcgg cgccgacccg tcggcgtcct acgtcgcgtc gctcaacgcg aaagggctcg
     1321 accacatcct cgagaaagtc gccgaggagg ccgtcgagac ggtgatcgcg tccaagaccg
     1381 gggagcgcgc cgcgatcgtc cacgagaccg cggacctctg gttccattgc ctcgtgatgc
     1441 tcgcgtggca cggcatcccc gccgccgaag tcctggcgga gctcgagcgg cgcgagggcc
     1501 gctccggaat cgacgagaag gcttcccgca cgaaaggagc gtcctgatgg ccgcgaaatc
     1561 cgactgcatc ttctgcaaga tcgtcgcggg cgccatcccg agccgcaagg tgtacgagga
     1621 cgacgacatg ctcgccttcc acgacatcaa cccggtcgct cccgtgcact tcatgatgat
     1681 ccccaaggac cacgtcgact cgctcgccac cgtgaccgac gcccatgccg cggtgctggg
     1741 caagatgatg gcgaaggcgg ggcgcctcgc gcgcgagcag ggcgcgaccg acggcttcag
     1801 gaccatcgtc aacacggggc ggatcggcag gcaggacgtg tatcatttgc acatacacat
     1861 catcggcggc cccgatcccc ttccacgcat gatcgcgaag gactaggcag gagctaaacc
     1921 atgggttcat tgagcatctg gcatttgttg atcctgttgt tggtcgtgct gctcatcttc
     1981 ggcaccaaga agctgcgcaa catgggcgcc gacatcggcg gcgcggtcaa gggtttcaag
     2041 gacggcatga agggcgaaga agaaagaaag ccgaccggcg agatcgcatc ctccgcgagc
     2101 accatcgaag gcgagatcaa ggaccggcag aagagctgaa gcggattcct ccggggcttc
     2161 tcgcgccccg cgccccgc
//
LOCUS       DASVII010000005         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_216120,
            whole genome shotgun sequence.
ACCESSION   DASVII010000005 DASVII010000000
VERSION     DASVII010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216120"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..719)
                     /locus_tag="VF309_00065"
     CDS             complement(177..719)
                     /locus_tag="VF309_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7465001.1"
                     /translation="MPMHKHLDADSIPRMRRRRNLLALFLAMALPCAAATLHEGKPAP
                     ALEAKLLDGQSFSLAAQSGKVVVINFWATWCAPCRAEMPALDAFYRKHRDEGLVVLAV
                     SLDEPDKEAKVREVMKAFGFPAALGPQSDFKGYMRIWRLPLTFVVDRSGILRKNDWYG
                     DPGLDEALLESTVAPLLRAR"
     gene            747..1112
                     /locus_tag="VF309_00070"
     CDS             747..1112
                     /locus_tag="VF309_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465002.1"
                     /translation="MRFAFRSTLFALAASTLASMALAYPAKMQDGVLANDAGMTLYTF
                     DKDAAGKSACNGPCAGNWPPLMATADAKASGDWSIVTRDDGGKQWAYKGKPLYTWSKD
                     AKPGDKTGDNFNNVWHVAK"
     gene            1146..1460
                     /locus_tag="VF309_00075"
     CDS             1146..1460
                     /locus_tag="VF309_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465003.1"
                     /translation="MKSTRRIAAALWLALALLVGQQAAALHDLGHATERLAQHQDSKT
                     PPASCDQCFACAELSSAVGVAPVVLPQVSAGVPVTCFVHDAAIFVAPRLAFRSQAPPT
                     LL"
     gene            1523..>2161
                     /locus_tag="VF309_00080"
     CDS             1523..>2161
                     /locus_tag="VF309_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465004.1"
                     /translation="MFRFVILPCACALVLLLAPPVRAADPELDEIRAQIRELKSSYEN
                     RIQALEQRLKDAEAKAAAPAPMPAAPSSSIGAFNPAVSVVLQGRYANLSQDPAKFALA
                     GFAPGGDIGPGRRGFSLGESELAMFANVDDKFAGNLIASLTPENTVSVEEAYGLWLTA
                     PYGLAPKFGRFFSGLGYLNEQHQHAWDFQDAPLVYKAFLGNQLKQDGLQLKWI"
BASE COUNT          361 a          723 c          700 g          377 t
ORIGIN      
        1 cggacatcga gaacagcggc gcgacgctcg tgtacttcag ccccggcgtg accgtgaagc
       61 tctcgcccaa gtcgagcgcc tacgggttcc tgcagctgcc gatctaccag agggtgaacg
      121 gcctgcagat cgagtcgcgc tactcggtgt ccttcggaat gcgcttcgcg ttgtagctag
      181 cgcgcgcgaa gcaggggtgc cacggtgctt tcgagcaggg cctcgtcgag cccgggatcg
      241 ccgtaccagt cgttcttgcg aaggatgccg gagcgatcga cgacgaacgt gagcggcagg
      301 cgccagatgc gcatgtagcc cttgaagtcg ctctggggcc cgagcgccgc gggaaagccg
      361 aacgccttca tcacctcgcg gactttcgct tccttgtccg gctcgtcgag gctgacggcg
      421 agaaccacca ggccctcgtc gcgatgcttg cgatagaacg cgtcgagcgc cggcatttcc
      481 gcgcggcacg gagcgcacca cgtggcccag aagttgatga cgacgacctt gcccgattgc
      541 gcggcgagcg agaaggattg cccgtcgagg agcttcgcct cgagcgcggg cgcgggcttt
      601 ccctcgtgaa gcgtggccgc cgcgcagggc agggccatcg ccaggaaaag cgcgagaaga
      661 ttgcgccgtc tcctcattcg tggaatggaa tcggcatcca gatgtttgtg cataggcatg
      721 ctcgataatc ctaacaggag aacctgatga gattcgcttt tcgctccacg ctgttcgccc
      781 ttgctgcttc gaccctcgcc tcgatggccc tcgcgtaccc cgccaagatg caggacggcg
      841 tgctggccaa cgatgccgga atgactctct ataccttcga caaggacgcg gcaggcaaga
      901 gcgcgtgcaa cggcccctgc gcggggaact ggccgcccct catggccacc gccgatgcca
      961 aggcctcggg cgactggtcg atcgtgacgc gcgacgacgg cggcaagcag tgggcgtaca
     1021 agggcaagcc cctctacacc tggtcgaagg acgcgaaacc cggcgacaag acgggcgaca
     1081 acttcaacaa cgtctggcac gtcgccaagt agcttttcgc ttcaggcggg tatcatgcgg
     1141 atagcatgaa atcgactcgc cgcatcgccg ccgcgctctg gctcgccctg gccctcctcg
     1201 tggggcagca ggcggcggcg ttgcacgacc tcggtcacgc gaccgagcgc ctcgcgcagc
     1261 accaggactc gaagactccg ccggcatcgt gcgaccagtg cttcgcttgc gcggagctct
     1321 cgagcgccgt gggcgtcgcg cccgtggtcc tgccccaggt atcggccggg gttccggtca
     1381 cgtgcttcgt tcacgacgcg gcgatcttcg tcgcgccgcg cctcgctttc cgctcgcagg
     1441 ccccacccac gcttctctga tgtcgttgcg gtccaccgcc gtcatggcgg tggcgtttgt
     1501 ccacgatacg aggaggttca ccgtgtttcg attcgtcatt ctgccgtgcg cgtgcgcgct
     1561 ggtgctgctg cttgcgccgc cagtgcgcgc ggccgatccg gaactcgacg agatccgtgc
     1621 ccagatccgc gagttgaaat cgagttatga aaatcgcatc caggcgctcg agcagcgcct
     1681 gaaggacgcg gaggcgaagg cggccgcgcc cgcacccatg cccgccgcgc cgtcgagctc
     1741 catcggcgcg ttcaatcccg ccgtctccgt cgtgctgcag ggacgctatg cgaacctttc
     1801 gcaggatccc gcgaagttcg ccctcgcggg cttcgcgccc ggaggggaca tcggtcccgg
     1861 ccgccgcggc ttcagcctcg gcgagtcgga gctcgcgatg ttcgcgaacg tggacgacaa
     1921 gttcgcgggc aacctcatcg catcgctcac gccggaaaac accgtctccg tcgaggaggc
     1981 ctacgggctg tggcttaccg cgccctacgg cctcgcgccg aagttcgggc gattcttctc
     2041 gggcctcggc tacctgaacg agcagcacca gcacgcgtgg gactttcagg acgcgccgct
     2101 cgtatacaag gcatttctgg gcaaccagtt gaagcaggac gggttgcagt tgaaatggat
     2161 c
//
LOCUS       DASVII010000006         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_288075,
            whole genome shotgun sequence.
ACCESSION   DASVII010000006 DASVII010000000
VERSION     DASVII010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288075"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VF309_00085"
     CDS             <1..1002
                     /locus_tag="VF309_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7465005.1"
                     /translation="GGSAAAVAARMAPAATGTDTGGSIRQPASFTGVCGLRPTYGRVS
                     RHGMVAFASSLDQGGALGKTAEDLAMMLEVMAGFDPRDSTCMDRAKDDYVGGLERGVK
                     GLRIGVPREYFSNDLARDVGEPILAAIDAYRRQGAAVVEVSLPNQHLSVPAYYVLAPA
                     EASSNLARYDGVRYGHRAAEYGDLLDMYCKTRAQGFGAEAKRRILIGTYVLSHGYYDA
                     YYLKAQQIRRLIARDFSEAFKACDVIMGPAAPCVAYDIGAKITDCVQMYLDDLYTIPI
                     NLAGLPGMSIPCGFGDRARPVGLQIVGDYWSEALMLSVAHAYQRATDWHTRAPAAFA"
     gene            999..>1815
                     /gene="gatB"
                     /locus_tag="VF309_00090"
     CDS             999..>1815
                     /gene="gatB"
                     /locus_tag="VF309_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7465006.1"
                     /translation="MNWEVVIGLETHAQLTTASKIFSGASTRFGASPNTQVSAIDLAM
                     PGTLPVLNKGAVERAIRFGLAVGGTIAPMSAFERKNYFYPDLPKGYQISQYVQPVVTG
                     GGVAIVVGDEERYVPLTRAHLEEDAGKLLHDAVAGRSGIDLNRAGTPLLEIVTEPAMR
                     SAAEAVAYAKALHSLVRWIGICDGNMQEGSFRCDANVSVRRRGDPLGTRCEIKNLNSF
                     RFLEKAIEYEVRRQVELIEDGGKVVQETRLFDPDRGETRPMRSKEDAQDYRYFP"
BASE COUNT          294 a          618 c          612 g          291 t
ORIGIN      
        1 ggcggatcgg cggccgcggt cgccgcgcgc atggcgccgg cggctaccgg caccgacacc
       61 ggcggatcga ttcgccagcc ggcatcgttc accggcgtgt gcgggttgag gcccacctac
      121 gggcgcgtgt cgcgccatgg catggtcgct ttcgcttcat cgctcgacca ggggggcgcg
      181 ctcggcaaga cggccgagga cctcgcgatg atgctcgagg tgatggcggg cttcgatccg
      241 cgcgactcga cctgcatgga ccgcgcgaag gacgattacg tgggcggcct tgagcgcggc
      301 gtgaagggcc tgcgcatcgg cgtgccgcgc gagtatttct cgaatgatct cgcccgcgac
      361 gtgggcgaac cgatcctcgc ggcgatcgac gcgtaccgca ggcagggcgc cgccgtggtc
      421 gaggtgagcc tgcccaacca gcacctttcg gtgccggcct actacgtgct ggctcccgcc
      481 gaggcatcgt cgaacctcgc gcgctacgac ggcgttcgct acggccaccg cgccgccgag
      541 tacggcgacc tcctcgacat gtactgcaag acgcgcgcgc agggcttcgg cgcggaggcg
      601 aagcggcgca tcctcatcgg cacctatgtg ctgtcccacg gctactacga tgcgtactac
      661 ctgaaggcgc agcagatccg ccgcctcatc gcgcgcgatt tctccgaggc cttcaaggca
      721 tgcgacgtca tcatggggcc ggcggcgccg tgcgtcgcct acgacatcgg cgccaagatc
      781 accgattgcg tgcagatgta cctcgacgat ctctacacga tcccgatcaa tctcgcgggc
      841 cttcccggga tgtcgatccc gtgcggcttc ggcgacaggg ctcgccccgt cggcctgcag
      901 atcgtcggcg actactggag cgaggcgctg atgctttccg tcgcgcacgc ataccagcga
      961 gccaccgact ggcacacccg cgcgcccgcg gcgttcgcat gaactgggaa gtcgtcatcg
     1021 ggctggagac gcacgcgcaa ttgaccaccg cgtcgaagat cttctcgggc gcctcgacgc
     1081 gattcggggc gtcgccgaac acccaggtct ccgcgatcga cctcgcgatg ccgggcacgc
     1141 tgccggtgct gaacaagggg gccgtcgagc gcgccatccg cttcgggctc gcggtcggcg
     1201 ggaccatcgc gccgatgagc gccttcgagc gcaagaatta tttctatccc gaccttccga
     1261 agggctacca gatcagccag tacgttcagc cggtcgtcac cggcggcggg gtggcgatag
     1321 tcgtcggcga cgaagagcgg tacgtgccgc ttacgcgcgc gcatctcgag gaggatgcgg
     1381 ggaagctcct gcacgacgcg gtcgccggcc gtagcggcat cgacctgaac cgtgccggaa
     1441 cgccgctcct cgagatcgtc accgagcccg cgatgcgctc ggccgccgag gccgtcgcct
     1501 atgcgaaggc gctgcactct ctcgtgcgct ggatcggcat ctgcgacggc aacatgcagg
     1561 aaggctcctt ccgttgcgac gcgaacgtat cggttcgacg ccgaggcgat cccctcggca
     1621 cgcgctgcga aataaagaac ctcaattcgt tccggttcct cgagaaggcg atcgagtacg
     1681 aggtgcgccg gcaggtggag ctgatcgagg atggcggcaa ggtcgtgcag gagacgcgcc
     1741 tcttcgatcc cgatcgcggc gagacgcgcc cgatgcgatc caaggaagac gcgcaggact
     1801 atcgctactt tcccg
//
LOCUS       DASVII010000007         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_467870,
            whole genome shotgun sequence.
ACCESSION   DASVII010000007 DASVII010000000
VERSION     DASVII010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467870"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..995
                     /locus_tag="VF309_00095"
     CDS             150..995
                     /locus_tag="VF309_00095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7465007.1"
                     /translation="MRTLVHISDLHFGRVDAALLAPLRERIEALAPHLVVVSGDLTQR
                     ATREQFREAKAFLDTLPQPQVVVPGNHDVPLYNVFQRVLSPLGKYLSIVTSDLEPSFI
                     DDEIAVVGVNTARSLTWKRGSIDRHQVDAAMNRIGAVRDRVAKIVVTHHPFDVPEGHA
                     ESNLVKHASHAMNVFAKCGADILLAGHLHHTRTSHSARRYRIPGHSALLVQAGTATSV
                     RGRGEPNSFNALHLTRDDVRIERWHWDGASRAFELGGSERFCRAPDGWHPESHFLETK
                     GLPRM"
     gene            complement(959..>1523)
                     /locus_tag="VF309_00100"
     CDS             complement(959..>1523)
                     /locus_tag="VF309_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7465008.1"
                     /translation="CRMRSRRLPRGFYDRDTILVAKELLGKHLVHVVDGVARVGRIVE
                     TEAYLGAHDLAAHSARGLTARTKPMFGPPGHAYVYLVYGMHWCMNVVTQPAGQASAVL
                     LRAVEPVRHVEGRTQGPALLCKAMGIDGRLTGHDLLSDDLYVVDPRDEAAIAIVKRPR
                     IGVDYARHWARRLLRFYIRGNPFVSRK"
BASE COUNT          243 a          546 c          502 g          232 t
ORIGIN      
        1 cattcgatcc gcatcgcgac caagcgaaag acgctgctgg tcgcgaccga cggcgaggtc
       61 acgcggatgc gcacgcccct cgagtaccgg atccgctcgc gcgggctgaa ggtcgtggtg
      121 cccgcggccg gacaagcctg aagggcgcga tgcgaaccct ggtccacatc tccgatctgc
      181 acttcggccg cgtcgacgcg gcgctgctcg cccccttgcg ggagcgaatc gaagcgctcg
      241 cgccccatct cgtggtggtt tccggcgacc tcacgcagcg cgcgacgcgc gagcagttcc
      301 gcgaggcgaa ggcattcctc gacacgctgc cgcaaccgca ggtcgtggtg cccggcaacc
      361 acgacgtgcc gctctacaac gtgttccagc gggtgctctc gccgctcggc aagtacttga
      421 gcatcgtgac cagcgacctc gagccctcct tcatcgacga cgagatcgcg gtcgtgggcg
      481 tgaatacggc gcgttccctg acctggaagc gcggcagcat cgaccggcac caggtggacg
      541 ccgccatgaa caggatcggc gccgtacgcg atcgcgtcgc gaagatcgtg gtgacgcatc
      601 atccgttcga cgtgcccgag ggccacgccg agtcgaatct cgtgaagcat gcgtcccacg
      661 cgatgaacgt gttcgccaag tgcggcgccg acatcctgct cgcggggcac cttcaccata
      721 cgcgcacctc gcattcggcc cggcgctacc ggatccccgg ccattccgcg cttctcgtgc
      781 aggccggcac cgcgacctcc gtgcgcggcc gcggcgagcc caacagcttc aatgccctcc
      841 acctcacgcg tgacgacgtg cgcatcgagc gctggcactg ggacggcgcc tcccgtgcct
      901 tcgagctcgg cggcagcgag cgcttttgcc gcgcccccga cggctggcac cctgaatctc
      961 atttcctcga aacgaagggg ttgccgcgga tgtagaagcg aaggaggcgc cgcgcccagt
     1021 ggcgcgcgta gtcgacgccg atgcgcgggc gcttcacgat ggcgatggcc gcctcgtcgc
     1081 gcgggtcgac gacgtacaga tcatcgctca gcagatcgtg tcccgtaagc cgcccgtcga
     1141 tgcccatggc cttgcaaagg agcgcggggc cctgggtgcg gccttccaca tgcctcacgg
     1201 gctccaccgc acgcagcagc acggcggacg cctgccccgc gggctgcgtc accacgttca
     1261 tgcaccagtg cattccgtag acgaggtaga cgtaggcgtg gccgggcgga ccgaacatcg
     1321 gcttggtgcg cgccgtgagg cccctggccg agtgcgcggc gaggtcgtgc gccccgaggt
     1381 acgcttccgt ctcgacgatg cgcccgacgc gagcgacgcc gtccaccacg tggacgaggt
     1441 gcttgccgag aagctccttc gcgacgagga tcgtgtcgcg gtcgtagaac ccccgcggaa
     1501 gccttcgcga tcgcatccgg caa
//
LOCUS       DASVII010000008         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_36514, whole
            genome shotgun sequence.
ACCESSION   DASVII010000008 DASVII010000000
VERSION     DASVII010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36514"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1352
                     /gene="ribBA"
                     /locus_tag="VF309_00105"
     CDS             231..1352
                     /gene="ribBA"
                     /locus_tag="VF309_00105"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX7465009.1"
                     /translation="MTISPTHELIDDIRRGRMVVLADAQDRENEGDLVLAADFVTPEA
                     INFLATFARGLVCLTLTEERCRQLNLPLMVNDNRSPMGTAFTVSIEAATGVSTGISAA
                     DRARTVRAATARHAKAEDVVQPGHIFPVMAQPGGVLVRAGHTEAGCDLAELAGLTPAA
                     VICEIMNADGSMARMPELLEFAKSHGLRIGTIADLIHYRSQTEKLVERVADRVVATPY
                     GAFRLCVYHDKTVDEVHYALVKGEPSPDRPILVRVHEPFVSLDLFDFDSARHAFSVQD
                     AMRMVSREKEGVIVLLRRSEPAAEILERLDESKARKNAARKWDPRLHGIGAQILKDLN
                     VGKMRVLARPKRIPSMSGFGLEIVEYVSPEDATAVRAAG"
     gene            1349..>1549
                     /locus_tag="VF309_00110"
     CDS             1349..>1549
                     /locus_tag="VF309_00110"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7465010.1"
                     /translation="MRLATVPPPDGKGLHVGIVWSRFNEDIVRELLTACDKELVELGV
                     DAADIDVVSVPGALEIPLALQAM"
BASE COUNT          253 a          541 c          523 g          232 t
ORIGIN      
        1 catcacgtgt ccgacgccct ggtcaccatg atcgtcgagc tcttcatcga gcgcttccgg
       61 ccggccgcat cgcaggtcta ggcggaatgc gagggctgga tacgcccgga gggagggcgt
      121 ccggcatcgg gattcgggac gcccagggag cttcacccag cggtcgacgc cgtccctccc
      181 ggcgaaaatg gcaaaatagc ggtctcgcca gtgcgccgaa gcccacttcc atgaccatca
      241 gcccgaccca cgaactcatc gacgacattc gccgcgggcg catggtcgtg ctcgccgacg
      301 cgcaggaccg cgagaacgag ggcgatctcg tgctcgccgc ggacttcgtg acgcccgagg
      361 cgatcaactt cctcgcgacc ttcgcgcgcg gcctcgtctg cctcacgctc accgaggaac
      421 gctgccggca gctgaacctg ccgttgatgg tgaacgacaa ccgctcgccc atgggaaccg
      481 cgttcaccgt ctccatcgag gccgcgaccg gcgtgagcac cggtatctcg gcggcggatc
      541 gcgcgcgcac cgtgcgcgcg gcgaccgcgc gtcacgcgaa ggcggaggac gtcgtgcagc
      601 cgggccacat cttcccggtg atggcgcagc cgggcggcgt cctggtgcgc gcgggccaca
      661 ccgaggcggg ctgcgacctc gccgagctcg ccgggctcac ccccgcggcg gtcatctgcg
      721 agatcatgaa tgccgacgga tccatggcgc gcatgcccga gctcctcgag ttcgccaaga
      781 gccacggcct gcgcatcggc accatcgccg atctcatcca ctatcgaagc cagacggaga
      841 agctcgtcga gcgcgtggcc gatcgcgtgg tggccacgcc ctacggggcg tttcgtctgt
      901 gcgtgtacca cgacaagacg gtcgacgagg tccactacgc gctggtgaag ggggagcctt
      961 cgccggatcg cccgatactc gtccgggtgc acgagccctt cgtctccctc gacctcttcg
     1021 atttcgactc cgcgcgccac gcgttctcgg tgcaggacgc gatgcgcatg gtgtcgcgcg
     1081 agaaggaagg cgtcatcgtg ctgctgcggc gctccgagcc ggcggccgag atcctcgagc
     1141 gcctcgacga atcgaaggcg cgcaagaacg ccgcccggaa atgggatccg cgccttcacg
     1201 gcatcggcgc gcagatcctg aaagacctga acgtcggcaa gatgcgcgtg ctggcgcgcc
     1261 cgaagcgaat cccgagcatg tcgggattcg ggctcgagat cgtcgaatac gtgtcccccg
     1321 aggatgccac cgcggtgagg gcggccggat gaggctcgcg accgttcccc cgcccgacgg
     1381 caagggcctc cacgtcggca tcgtctggag ccgcttcaac gaggacatcg tgcgcgagct
     1441 cctcacggcg tgcgacaagg agctggtcga gctgggcgtc gatgcggccg acatcgacgt
     1501 ggtgagcgtc cccggcgcgc tcgagatccc gctggcgctg caggcgatg
//
LOCUS       DASVII010000009         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_575891,
            whole genome shotgun sequence.
ACCESSION   DASVII010000009 DASVII010000000
VERSION     DASVII010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575891"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VF309_00115"
     CDS             complement(<1..235)
                     /locus_tag="VF309_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7465011.1"
                     /translation="MRHPVEVDAKDQDHLVKWLSKRLDMALTVPVLSTEGFELLGGRL
                     LPGNDGPVAQFMYQDASGKRLTLYVTRPHKGDTV"
     gene            297..674
                     /locus_tag="VF309_00120"
     CDS             297..674
                     /locus_tag="VF309_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7465012.1"
                     /translation="MRHPVEVEAKDQEHLVKWLSKRLDMQLRIPVLAGEGFELLGGRL
                     LPGTDGPVAQFMYQEATGKRLTLYVTRPHKGDTVTAFRFAQEGPVSVFYWIDRDCGYA
                     LSGDIDKPALARIATSVYKQLEP"
     gene            777..1832
                     /locus_tag="VF309_00125"
     CDS             777..1832
                     /locus_tag="VF309_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7465013.1"
                     /translation="MSDIDKHEHAEPARGPWPEPRLKALQVDEIYALAPSATAFSYFG
                     ALLTLGVLIQSGEIGRGAIWFLWATAVTFFRSTCIVAYRRRSPDSDPEAWGRLVVAAN
                     FLAGVQWGVLGTLLFPEGPAYLQLFTLMVIICFVTGSVTAYAAVKGAHPALSIPATVP
                     TSIYLFFVQDGVHWYEGVMALFFCFAIVFYARRLHRHLEQGFRLQIERDDLLSVTGIL
                     NEKLERENRDLTHRAAVRGVSAESARERADRLEALFERSALPQIECDAAGNILVCNPA
                     TERLFGLRRDEIAGRSLMTFLRIPGSDLKSLASAPQGEIFDVEALGRAGTRKCRASIT
                     PLPATGDRLPGFGVVLV"
BASE COUNT          312 a          715 c          633 g          365 t
ORIGIN      
        1 tgaccgtatc gcccttgtgg gggcgcgtca cgtagagcgt gaggcgcttg ccggacgcgt
       61 cctggtacat gaactgcgcg accggcccgt cgttgcccgg cagcaggcgc ccgccgagga
      121 gctcgaagcc ctcggtggaa aggaccggga ccgtgagcgc catgtccagg cgcttggaga
      181 gccatttcac gagatggtcc tggtccttgg cgtcgacttc caccgggtga cgcacctccg
      241 gggaatagac gaggtgcgct cgcgccgcgc gcgcgcacct cgtctattcg ccggaggtgc
      301 ggcaccccgt cgaggtcgag gcgaaagacc aggagcacct cgtgaagtgg ctgtcgaagc
      361 gcctcgacat gcagctgagg attccggtgc tcgcgggcga gggcttcgag ctcctcggcg
      421 gccggctact gccgggcacc gacgggccgg tcgcgcagtt catgtaccag gaagccaccg
      481 gcaagcgcct cacgctctac gtcacgcgcc cccacaaggg cgatacggtc accgcattcc
      541 gcttcgctca ggaaggcccg gtctcggtgt tctactggat cgatcgcgac tgcggctacg
      601 cgctctccgg cgatatcgac aagcccgccc tcgcgcggat cgccacctcg gtctacaagc
      661 agctcgagcc ctaggctcct ggattgtttc cgttgtaatc ggccgctgtg acgcaaatca
      721 cagccgcccc gggataatgc gctcaccctg accgatggcg cctcgcggga cgtttcatgt
      781 cggacatcga taaacacgag cacgcggaac ccgcacgggg accgtggccc gagccgcgcc
      841 tgaaggctct gcaggtcgac gagatctacg ctctcgcccc ctcggcgacg gcgttctcgt
      901 acttcggcgc gctcctcacg ctcggcgtcc tcatccagtc gggcgagatc ggccgcggcg
      961 cgatctggtt cctgtgggcc accgccgtca ccttcttccg ctcgacgtgc atcgtcgcct
     1021 atcgccgccg tagccccgac tcggatccgg aagcgtgggg acgcctcgtg gtcgcggcga
     1081 atttcctcgc gggcgtgcag tggggcgtcc tggggacgct gctctttccc gaagggcccg
     1141 cgtacctgca gctcttcacg ctgatggtca tcatctgctt cgtcacggga tcggtcaccg
     1201 cctacgccgc cgtcaagggc gcgcacccgg cgctttccat tccggcgacc gtgcccactt
     1261 cgatctacct tttcttcgtg caggatgggg tgcattggta cgagggcgtg atggcgctct
     1321 tcttctgctt cgcgatcgtg ttttacgcgc gccggctcca tcgccatctc gagcagggct
     1381 tccgcttgca gatcgagcgc gacgatctgc tgtcggtcac gggaatcctc aacgaaaagc
     1441 tcgagcgcga gaatcgcgac ctcacccatc gcgctgccgt gcgcggggtc tccgccgaga
     1501 gcgcgcggga acgcgccgat cgcctcgagg cgctcttcga acgctcggcg ctgccgcaga
     1561 tcgagtgcga cgccgccggc aacatcctgg tctgcaatcc cgcgaccgag cgcctcttcg
     1621 gcctgcgccg cgacgagatc gcgggccgct cgctcatgac tttcctgcgc atccccggct
     1681 ccgacctgaa gtccctcgcc tccgcgcccc agggcgaaat cttcgacgtc gaggccctcg
     1741 gccgcgccgg cacccgcaag tgccgcgcga gcatcacgcc gctccccgcc accggcgatc
     1801 gcctcccggg cttcggagtc gtcctcgtct agaattggtg tctaaaactg gtgtcagaga
     1861 ccaatttcca aggggtggaa attggtctct gacaccagtt ttgcaaagcg tacctgacac
     1921 caattcaatg acctcgcttc cccccgaaat tctctccgga acgctcgccg cggatcatcc
     1981 caggatcgcg gcgctgacgg cgtcgatcga caagcgcgcg aggga
//
LOCUS       DASVII010000010         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_503935,
            whole genome shotgun sequence.
ACCESSION   DASVII010000010 DASVII010000000
VERSION     DASVII010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503935"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VF309_00130"
     CDS             <1..1510
                     /locus_tag="VF309_00130"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7465014.1"
                     /translation="AKIKLGGGEEKIAAQHEREKLTARERIALLIDDGTFVELGIHGT
                     PHSSQRAMDGVDAPADGVITGYGKVDGRLTAVCAYDFTVMAGSMGMTGEFKVTRLREL
                     ALTKRMPFVWLLDSAGARVQEAAGAMFAGSGHLFREEVVMSGVIPQVAALMGPCAAGT
                     AYIPGLADFVPMVKGRGSMALAGPHLVKAAIGEDVTQEELGGSRVHCRKSGVGDMEVA
                     SDEECIERIKQYLSFMPQNCEERPPIRAVVDPIDRREEELLDVLPESNRKPYDMYEVI
                     KRIVDDGEYFDMKGQWAKTIITCFARFGGRPVGIVANQPKQLGGILDNDSADKAARFV
                     NLCNAFGIPLLFIQDVPGFMVGTKVEAEGIIRHGAKMLYAVANATVPKITVITRKAYG
                     AGYYVMNGRAYEPDLIVAWPSAEISVMGAEGVVEIAFRKQVEAAEDPAAKKAELIEGF
                     RKIFDVYVAAGNDVIDDVIDPRETRETICRALEMSENKKIERPWKRQGVVPV"
     gene            1507..>2319
                     /locus_tag="VF309_00135"
     CDS             1507..>2319
                     /locus_tag="VF309_00135"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX7465015.1"
                     /translation="MSLDHPVVITCSISGAIANREQCPAIPYTPAEYAAEARRIVDEG
                     GVHIHIHARKPDGTPSYEIEDFQAITDAIRGEVGDAAIINYSTGTIGVPVAKRIEYLR
                     ACPPEVAALNMGSMNYAKYSKSRKQFVFSMVFENPFAEIIELLEAMNELNIKPEHECF
                     DIGHVGSLAPLIDMGVLKTPLHVDCVMGVVGGIPPTARNLAAMVDNIPEGSHWGVIGI
                     SRDQWMLVSAALTLGGSIRVGLEDNLYLPNGGMAKSNGELIAKARQMTEDMGR"
BASE COUNT          427 a          744 c          804 g          344 t
ORIGIN      
        1 cgcgaagatc aagctcggcg gcggcgagga gaagatcgcc gcgcagcacg agcgcgaaaa
       61 gctcaccgcg cgcgagcgca tcgcgctgct catagacgac ggcacgttcg tcgagctcgg
      121 catacacggc acgccgcact cctcccagcg cgcgatggac ggcgtggacg cgcccgcgga
      181 cggcgtgata accggctacg gcaaggtcga cggccggctg acggccgtgt gcgcgtacga
      241 cttcacggtg atggcgggct cgatggggat gaccggcgag ttcaaggtga cgcgtctgcg
      301 cgagctggcg ctgaccaagc ggatgccgtt cgtgtggctg ctggactcgg cgggcgcgcg
      361 cgtgcaggag gcggcgggcg cgatgttcgc gggctcgggg cacctgttcc gcgaggaggt
      421 cgtcatgagc ggcgtgattc cccaggtggc cgcgctgatg ggcccgtgcg ccgccggcac
      481 cgcgtacatc cccggtctcg ccgacttcgt gccgatggtc aagggccgcg gctcgatggc
      541 gctcgccggg ccccatctgg tcaaagcggc gatcggcgag gacgtcaccc aggaggaact
      601 cggcggctcg cgcgtgcact gccgcaagtc gggagtgggg gacatggagg tcgcaagcga
      661 cgaggagtgc atcgagcgga tcaagcagta cctgtcgttc atgccgcaga actgcgaaga
      721 gcgccctccg atccgcgctg tggtcgaccc gatcgaccgc cgcgaggagg agttgctcga
      781 tgtgctgccg gagtccaacc gcaagccgta tgacatgtac gaggtgatca agcggatcgt
      841 cgacgacggc gagtacttcg acatgaaggg ccagtgggcg aagacgatca tcacgtgttt
      901 cgcgcgcttc ggcggtcggc ccgtcgggat cgtcgccaac cagcccaagc aactcggcgg
      961 catcctcgac aacgactccg ccgacaaggc cgcccgcttc gtcaacctct gcaacgcgtt
     1021 cggcattccg ctgctgttca tccaggacgt gcccggcttc atggtcggca cgaaggtcga
     1081 ggccgaaggc atcatccgcc acggcgcgaa gatgctctac gcggtcgcca acgcgaccgt
     1141 gccgaagatc acggtgatca cgcgcaaggc ctacggtgcg ggctactacg tgatgaacgg
     1201 ccgcgcctac gaaccagacc tgatcgtcgc ctggccgagc gcggagatca gcgtgatggg
     1261 cgccgagggg gtcgtggaga tcgcgttccg caagcaggtc gaagcggccg aggaccccgc
     1321 cgcgaagaag gccgaactga tcgagggctt ccgcaagatc ttcgacgtgt acgtagccgc
     1381 gggcaacgac gtgatcgacg acgtgatcga cccgcgcgag acacgcgaga cgatctgccg
     1441 cgcgctcgag atgtcggaga acaagaaaat cgagcggccg tggaagcggc agggagtggt
     1501 ccccgtatga gcctcgacca tcccgttgtg atcacctgct cgatctcggg cgcgattgcc
     1561 aaccgcgagc agtgccccgc gattccgtac acgcccgctg agtacgccgc ggaggcgcgg
     1621 cggatcgtgg acgagggcgg cgtgcacatc cacatccacg cgcgcaaacc cgacgggacc
     1681 ccgtcgtatg agatcgagga cttccaggcg atcaccgacg cgatccgggg cgaggtcggc
     1741 gacgcggcga tcatcaacta ctccaccggc acgatcggcg tgcccgttgc caagcgcatc
     1801 gaatacctgc gcgcgtgccc tcccgaagtg gccgcgctga acatgggctc gatgaactac
     1861 gccaagtact ccaagtcgcg caaacagttc gtcttctcga tggtgttcga gaacccgttc
     1921 gcggagatca tcgagctgct ggaggcgatg aacgagctga acatcaaacc cgagcacgag
     1981 tgcttcgaca tcggccacgt cggcagcctc gcgccactga tcgacatggg cgtgctgaaa
     2041 accccgctgc acgtggactg cgtgatgggc gtcgtcgggg gcatcccgcc gaccgcgcgc
     2101 aacctcgcgg cgatggtcga caatattccg gaggggtcgc actggggcgt gatcggcatc
     2161 tcgcgtgacc agtggatgct cgtgtccgcc gcgctcacgc tcggcggctc gatccgcgtg
     2221 ggcctcgagg acaacctcta cctccccaac ggtgggatgg cgaagtccaa cggcgagctg
     2281 atcgcgaagg cgcgccagat gaccgaggac atgggccgt
//
LOCUS       DASVII010000011         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_396150,
            whole genome shotgun sequence.
ACCESSION   DASVII010000011 DASVII010000000
VERSION     DASVII010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396150"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1481
                     /locus_tag="VF309_00140"
     CDS             51..1481
                     /locus_tag="VF309_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7465016.1"
                     /translation="MSLGITSKLFIAFLVTNVITAFAVGLGVRATFNSGFEAYVQERE
                     AQRLARLAAALGDAYREKQGWDFLRDNDAMWLEMNRAVRSERRYGPVPQGPPPDRNDP
                     NSAIRRAPREPRPPRAVVFDAAQKVIVGDADPASQLDRRPIQSNGVVVGWLAAPIQQT
                     AFDFVDHRFQQQQWRASASVALAAMALAAVVAWFLSRGLIAPVKRLAGATRKLADGEY
                     ATRVASTSNDELGRLVDDFNRLGNALEKHEVSRRNFMADVSHELRTPLAVLKGELEAL
                     EDGVRPISRERIQSLQAEVGLLAKLVNDIHDLSLADVGGLAYHFKPADLGGIVRDAVR
                     IAQPRFIARALATELRLPSGAMPVRGDEERLAQLLGNLVENSLRYTDPGGALRVSMRR
                     EGSHAVIDWEDSKPGVPAEALPRLFERLFRVDNSRNRGRGGSGLGLAICRSIAEAHGG
                     SIEARASELGGLRIVLRLPLAVGDAA"
     gene            1478..2146
                     /locus_tag="VF309_00145"
     CDS             1478..2146
                     /locus_tag="VF309_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465017.1"
                     /translation="MNERVLIVEDEPKLAKLLDDYLRASGYRTDCVDDGRDVVAAVRR
                     NPPDLILLDLMLPGLGGLEVFKAVRAFADIPIVMVTALVEEVDRLVGLELGADDYICK
                     PYSPREVVARVKAVLRRGARGREDKVAGLTLDESRYEALLDGALLDLTPVEFRLLKTL
                     ASRPGRIFPRAELLDSLYADHRIVADRTVDSHVKNLRRKLQKVRAGEDLVHSVYGVGY
                     KLEF"
BASE COUNT          320 a          779 c          746 g          335 t
ORIGIN      
        1 cccgatgcac gaaaatgggc ctgtcttttc atcagccgta actatttccg gtgtccctcg
       61 gcatcacctc gaagctgttc atcgccttcc tcgtgacgaa cgtcatcacc gcgttcgccg
      121 tggggctcgg cgtacgggcg accttcaact ccggcttcga ggcctacgtc caggagcgcg
      181 aggcgcagcg cctcgcccgc ctcgcggcag cgctcggcga cgcctaccgc gagaagcagg
      241 gctgggactt cctcagggac aacgacgcga tgtggctcga gatgaatcgc gcggtgcgtt
      301 ccgagcgccg atacggcccg gtcccgcagg gaccgcctcc cgaccgcaac gacccgaaca
      361 gcgccattcg ccgcgcgccg cgcgagccgc gacccccgcg cgcagtggtg ttcgatgccg
      421 cgcagaaggt gatcgtcggc gacgccgatc ccgcatcgca gctcgatcgc cgcccgatcc
      481 agtcgaacgg ggtcgtggtg gggtggctcg ccgcgcccat ccagcagacg gcgttcgatt
      541 tcgtcgacca tcgcttccag caacagcaat ggcgcgcgag cgcgagcgtg gcgctcgccg
      601 cgatggcgct cgccgcggtg gtcgcgtggt tcctttcgcg agggttgatc gcgccggtca
      661 agcgcctggc cggcgccacc cgcaagctcg ccgacggaga gtacgcgacg cgggtggcct
      721 cgacttccaa cgacgagctg ggccgcctcg tcgacgactt caaccgcctc ggcaacgcgc
      781 tcgagaagca cgaggtgtcg cggcgcaact tcatggccga cgtctcccac gagctgcgca
      841 cgccgctcgc ggtgctgaag ggcgagctgg aagcgctcga agacggcgtg cgcccgatct
      901 cgcgcgagag gatccagtcg ctccaggcgg aggtgggcct cctcgcgaag ctggtgaacg
      961 acatccacga cctttcgctc gccgacgtgg gcggcctcgc ctatcacttc aagcccgccg
     1021 acctcggggg catcgtgcgc gacgcggtgc ggatcgcgca gccgcgcttc atcgctcgcg
     1081 cgctggcgac cgagctgcgc ctgccgtcgg gcgcgatgcc ggtgcgcggc gacgaggagc
     1141 gcctcgcgca gcttctcggc aatctcgtcg agaattcgct gcgctacacg gatcccggcg
     1201 gcgcgctgcg cgtgtcgatg cgccgcgaag gcagccacgc ggtgatcgac tgggaggatt
     1261 cgaagccggg cgttcccgcc gaggcgctgc cgcgcctgtt cgagcggctc tttcgcgtcg
     1321 acaactcgcg caatcgcggg cgcggcggct ccggcctcgg gctcgcgatc tgccgcagca
     1381 tcgccgaggc gcacggcggc tcgatcgaag cgcgcgcctc cgagctgggc ggccttcgca
     1441 tcgtgctgcg cctgcccctt gccgtcgggg acgcggcgtg aacgagcgcg tgctcatcgt
     1501 cgaggacgag ccgaagctcg cgaagctcct cgacgattac ctgcgggcct ccggataccg
     1561 gacggattgc gtcgacgacg ggcgcgacgt cgtcgccgcc gtgcggcgaa accctccgga
     1621 cctcatcctc ctcgatctca tgctgccggg cctgggcggc ctcgaggtgt tcaaggcggt
     1681 gcgcgcgttc gccgacattc ccatcgtgat ggtgacggcg ctcgtcgagg aagtggaccg
     1741 cctcgtcggg ctcgagctcg gcgccgacga ctacatctgc aagccctaca gcccgcgcga
     1801 agtggtggcg cgcgtgaagg cggtgctgcg ccgcggcgcg cgcgggcgcg aggacaaggt
     1861 ggccggtctc acgctcgacg agtcgcgcta cgaggccctg ctcgatggcg cgctcctcga
     1921 cttgacgccg gtggagttcc gcctgctgaa gacgctcgcg tcacgcccgg gccgcatctt
     1981 cccgcgcgcc gagcttctcg acagcctcta cgccgaccat cgcatcgtcg ccgaccgcac
     2041 cgtcgacagc cacgtgaaga acctgcgccg caagctgcag aaagtgcgcg cgggcgaaga
     2101 cctcgtgcac tcggtctacg gagtcggcta caagctcgag ttctaactac aaggggttcc
     2161 acaaggcgcg ctggatcgcg
//
LOCUS       DASVII010000012         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_72535, whole
            genome shotgun sequence.
ACCESSION   DASVII010000012 DASVII010000000
VERSION     DASVII010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72535"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VF309_00150"
     CDS             complement(<1..706)
                     /locus_tag="VF309_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX7465018.1"
                     /translation="MRILLLGASGFIGREILAALAARGHQVVPAVRGAASAPPFASER
                     AIVVDLDRDIAAGTWLPRLDGIDAVVNCAGILQGTRTHSIDAIHRLSPIALFDACAQA
                     GVRRVIQVSAISADRGAGTDYALTKLAADDHLRASSLDWIVVRPSLVVARGAFGGTAL
                     LRAMAALPFAIPVPMAGDQRFQPIHVDDLAALIVKALEGDALVRRTLDAVGPEVVTLR
                     ALLEHYRRWLGFEAVPV"
     gene            complement(708..>1585)
                     /locus_tag="VF309_00155"
     CDS             complement(708..>1585)
                     /locus_tag="VF309_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7465019.1"
                     /translation="LRPLARLSLGANHAVGAFLGRLVYFWSPRYRMRVLDNLAASGLA
                     PSPDDVRRLAKANAAEIGKGATELVWALFRPIDEVAATVVDRVGWEAVEKLRAAGRAI
                     LFVTPHLGSYDIAGRYLWTHLPILAMYRPHKVFWLDQMMREGRNRGAAPDGSNTAPAT
                     LAGVRMVLKHLRRGGCSIVLPDQVPGLGEGEWVDFFGRPAYTMTLVGRLQQASDAALV
                     FCYAERLPAGAGFRLHLEPLEGALPADRRDAARKVNEMVEKLVRECPAQYLWGYNRYK
                     RPAGAPAAPAPSGAA"
BASE COUNT          251 a          548 c          593 g          193 t
ORIGIN      
        1 ccacgggaac cgcctcgaag ccgagccagc ggcgatagtg ctcgaggagc gcgcgcagcg
       61 tgacgacctc gggacccacg gcgtcgaggg tgcgccgcac gagcgcatcg ccttccaggg
      121 ccttcacgat gagcgcggcg agatcgtcga cgtggatcgg ctggaagcgc tggtcgcccg
      181 ccatcggaac gggaatcgcg aagggaagcg ccgccatcgc gcgcaggagc gcggtgccgc
      241 cgaaggcgcc gcgcgcgacg acgagcgagg ggcgcaccac gatccagtcg agggagctcg
      301 cgcgcaggtg gtcgtcggcc gcgagcttgg tgagagcgta gtcggtgccg gcgccgcgat
      361 cggcgctgat ggccgagacc tggatcacgc gccgcacgcc ggcctgcgcg cacgcatcga
      421 agagcgcgat cggcgagagg cgatggatcg cgtcgatcga atgggtccgc gtgccctgca
      481 ggatgcccgc gcagttcacc acggcgtcga tgccgtcgag ccgcggcaac caggtcccgg
      541 cggcgatgtc gcgatcgaga tcgacgacga tcgcgcgctc gctggcgaag ggcggcgccg
      601 acgcggcgcc gcgcaccgcg ggcaccacct ggtgcccgcg cgccgcgagg gcggcgagaa
      661 tctcgcggcc gatgaatccc gaggcgccga gaaggaggat gcgcatctca ggccgcaccc
      721 gaaggcgccg gcgcggccgg cgcgcccgcg gggcgcttgt agcggttgta gccccacagg
      781 tattgcgcgg ggcactcccg cacgagcttc tccaccatct cgttcacctt gcgcgccgcg
      841 tcgcggcgat cggcgggcag cgcgccttcg aggggctcga ggtgcagcct gaagcccgcc
      901 cccgcgggca ggcgctcggc gtagcagaac acgagcgcgg catcggaggc ctgctgcagg
      961 cggcccacga gggtcatggt gtacgccgga cgcccgaaga aatcgaccca ttcgccttcg
     1021 cccaaccccg ggacctggtc ggggagcacg atgctgcagc cgccgcggcg caagtgcttc
     1081 agcaccatgc gtacgcccgc gagcgtcgcg ggcgcggtgt tcgagccgtc gggagccgcc
     1141 ccgcggttgc gcccttcgcg catcatctgg tccaaccaga acaccttgtg ggggcggtac
     1201 atcgccagga tcggcaggtg ggtccagaga tagcgcccgg cgatgtcgta gctgccgagg
     1261 tgcggcgtga cgaagaggat ggcccgcccc gccgcgcgca gcttctcgac cgcttcccag
     1321 cccacgcggt ccaccaccgt cgcggcgact tcatcgatgg gacggaagag cgcccaaacg
     1381 agctcggtcg cccctttgcc gatctcggcg gcgtttgcct tcgccaggcg gcgcacgtcg
     1441 tcgggagagg gcgcaaggcc gctcgcggcg aggttgtcga ggaccctcat ccggtagcgc
     1501 ggcgaccaga aatagactaa tcggcccagg aaagcgccca cggcgtggtt ggctcccagc
     1561 gagaggcgcg cgagcgggcg aagga
//
LOCUS       DASVII010000013         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_216373,
            whole genome shotgun sequence.
ACCESSION   DASVII010000013 DASVII010000000
VERSION     DASVII010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216373"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..930)
                     /locus_tag="VF309_00160"
     CDS             complement(160..930)
                     /locus_tag="VF309_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7465020.1"
                     /translation="MRMWNPANAIVVVALLMGPLAASAQLKVMISGGFSGPYEKLLPE
                     FERTTGIKVTTSSGSSQGTGPQTIGAQLARGVPVDVVILSREGLAEQIAAHRIVPGSD
                     VDLARTPIGVAVRSGAAKPDVTTVEAFKQVVLNAKAVAVPASTSGIFLTKDVLPRLGI
                     ADKVNVKVATRGTGAAAMVASGEADIGLLPVSEIVHAPGVELAGVIAEEIQLNQVFAA
                     AVVAGSNEVESAKRLIAFFASEHASAAIKGGGMEPLAK"
     gene            complement(985..1764)
                     /locus_tag="VF309_00165"
     CDS             complement(985..1764)
                     /locus_tag="VF309_00165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7465021.1"
                     /translation="MPSANSLRALYEKHEGKLADKWSLYLDVYERVLAPYRDRPIAFV
                     EVGVQNGGSLELWAKYFADARVVIGCDANPACGALRFDDPRIALLIGPINSPPVYREI
                     TARAPQIDVFIDDGSHHSRDIVNSFCNYFQHVAPGGLYIVEDLHCAYFRDWGGGYSEP
                     AGAMGFLKLLADGLHHAYWKDQASFEAQVVPFLPPGAGMGASLAADIFAITFFDSMCV
                     IEKRAAGAEAGLGERVLAGREAPIDPRPLEMRGSIVRPSRR"
BASE COUNT          311 a          624 c          580 g          324 t
ORIGIN      
        1 catagagcca taacgtcgga ctatcgaggc tgtacccgct ccgccccgaa aagtagtcgc
       61 ccacgatcgt tccgaccacg attccgagcg acacacctgc cgcaatgaga gcggacttcg
      121 ctgctatggg caatcgcttc caggcgttgt caggtgtcat cacttcgcga gcggctccat
      181 cccgccgccc ttgattgccg ccgacgcgtg ctcggaggcg aagaatgcga tcaggcgctt
      241 cgccgactcg acctcgttcg atcctgccac cactgccgcc gcgaacacct gattcagctg
      301 gatctcctcc gcgatcacgc cggcgagctc gacacccggt gcatgcacga tttcgctgac
      361 cgggaggagc ccgatatccg cttcacctga cgcgaccatg gccgcagcgc ccgtgccgcg
      421 cgtcgcgacc ttgacgttga ccttgtccgc gatgccgagc cgcggcaaga catccttcgt
      481 gaggaagatg ccgctcgtgc tggccgggac cgcgacggct ttcgcgttca atacgacttg
      541 cttgaaagct tctacggtgg tgacgtcggg cttcgcggcc ccggagcgca ctgccacgcc
      601 gatgggcgtt cgcgccaggt ccacatcgct tccggggacg attctgtgcg cggcgatctg
      661 ctcggcgagg ccttccctgg agagaatcac cacgtcgacg ggcacgccgc gggcgagctg
      721 cgccccgatg gtctgcggcc ccgttccctg cgacgagcct gagctggtgg tgaccttgat
      781 gcccgtcgtg cgctcgaatt ccggcaagag cttttcgtac gggccggaga atccgcccga
      841 tatcatcacc ttcaattgtg ccgacgcggc gagcgggccc atcagcagcg cgacgacgac
      901 gattgcattc gctggattcc acatgcgcat gtcatttctc cgtattgact cttgatcacc
      961 tcgcggagca tcgccaggac cggcctagcg gcgcgatggc ctgacgatac tcccgcgcat
     1021 ctcgagcggc ctgggatcaa tcggcgcttc gcgccccgcg agcacccgct cgcccagccc
     1081 ggcttcggct cccgcggcgc gcttctcgat cacgcacatc gaatcgaaga acgtgatcgc
     1141 gaaaatgtcc gcggcgaggc tcgcgcccat gcccgcgccc ggcggcagga aaggcacgac
     1201 ctgcgcctcg aacgaggcct ggtccttcca gtacgcgtgg tgcaggccat cggcgagcag
     1261 cttgaggaaa cccatcgccc ccgcgggctc ggagtacccg ccgccccagt cgcggaaata
     1321 ggcgcagtgc aggtcttcga cgatgtagag cccgccgggc gccacgtgct ggaagtagtt
     1381 gcagaaggag ttgacgatgt cgcgcgaatg gtgcgagccg tcgtcgatga agacgtcgat
     1441 ctgcggagcc cgcgcggtga tctcacgata gaccggcggc gagttgatgg ggccgatcaa
     1501 gagcgcgatg cgcgggtcgt cgaagcgcag cgcgccgcac gccgggttgg cgtcgcagcc
     1561 gatgacgacg cgcgcatccg cgaagtactt cgcccagagc tccagcgagc cgccgttctg
     1621 cacgcccacc tcgacgaacg cgatcggccg atcacgatac ggcgcgagca cgcgctcgta
     1681 gacatcgagg tagaggctcc atttgtcggc gagcttgcct tcatgctttt cgtatagggc
     1741 gcgcaggctg ttggcggaag gcatatccgt tggctcgttt ggtgcgcccg cgctaacgat
     1801 cgcgttgtca ggctcgtttc gcacggaagc ccaactctc
//
LOCUS       DASVII010000014         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_72639, whole
            genome shotgun sequence.
ACCESSION   DASVII010000014 DASVII010000000
VERSION     DASVII010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72639"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1372)
                     /locus_tag="VF309_00170"
     CDS             complement(20..1372)
                     /locus_tag="VF309_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX7465022.1"
                     /translation="MAQREGSLEAPTRHPIDWKSPEYRDEGKILAEAERVFEICHGCR
                     RCVSLCNTFPKLFDFIDAGPGGEIDGVPKEKYWEVVDQCYLCDLCYVVKCPYVPPHPW
                     NVDFPHLMLRAKAYQFEEGKMKHPVRDRALSSTDRNGKLATIPVVVQVVNAASHSKPL
                     RALGEKVAGVDKDAWVPEFTARKFRPNAAASKAHPVKNGALTPGRVAIFSTCYVNYNE
                     PGIGHDLLKILDHNGIAYVLVEKEACCGMPKLEQGDLGSVERLKEQNIPHLAKLAREG
                     YAILTPVPSCTLMFKQELPLMFPDDEDVKAVRDAMFDPFEYLVYRRRDGLLKTEYPRA
                     LGNVAYHVPCHSRVQKIGLKTKECLEAVPGTQVTVIERCSGHAGTWGVKAEFHDISLK
                     IGRPIFRQVAELAPDFFSSDCQLAGHHIEEGLGTVEKTNAPQLKHPITLLRMAYGLPD
                     "
     gene            complement(1377..1799)
                     /locus_tag="VF309_00175"
     CDS             complement(1377..1799)
                     /locus_tag="VF309_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX7465023.1"
                     /translation="MKPLKGTKTHDNLKAAFAGESQANRRYLYFANKADVEGQNDVSA
                     IFRSTAEGETGHAHGHLDYLAAVGDPATDLPIGSSRQNLKAAIAGETHEYTDMYPGMA
                     KSARTEGFDEIADWFETLAKAERSHANRFQKALDQLTD"
BASE COUNT          313 a          644 c          666 g          394 t
ORIGIN      
        1 tcacgggaaa gtttcggcat cagtcgggaa gaccgtacgc catcctgaga agcgtaatgg
       61 ggtgtttgag ttgcggagcg ttggtcttct ccacggtgcc cagcccttcc tcgatgtggt
      121 ggccggcgag ctggcagtcg gacgagaaga aatccggggc gagctcggcc acctggcgga
      181 agatgggccg cccgatcttc agagagatgt cgtggaactc ggctttcacg ccccaggttc
      241 ccgcatggcc cgagcagcgc tcgatcaccg tgacctgcgt gccgggcacc gcttcgagac
      301 attccttcgt cttcaggccg atcttctgca cgcgcgagtg gcacggcacg tgataggcga
      361 cgttgccgag cgcgcgcggg tattcggtct tcagcaaccc gtcgcgacgg cgataaacga
      421 gatactcgaa gggatcgaac atcgcgtcgc gcaccgcctt cacgtcctcg tcgtccggga
      481 acatgagcgg cagctcctgc ttgaacatca gcgtgcacga gggcaccggc gtcaatatcg
      541 cgtagccctc gcgcgcgagc ttcgcgaggt gggggatgtt ctgctccttc aatcgctcga
      601 ccgacccgag gtcgccctgc tcgagcttcg gcattccgca gcacgcctcc ttctcgacca
      661 gcacgtaggc aatcccgttg tggtcgagga tcttcaggag gtcgtggccg atgccgggct
      721 cgttgtagtt cacatagcac gtggaaaaga tcgcgacgcg ccccggcgtc agcgcgccgt
      781 tcttcaccgg atgcgccttg ctcgccgcgg cattcggccg gaacttgcgc gccgtgaact
      841 ccgggaccca cgcatccttg tccacgccgg ccaccttctc gcccagcgcg cgcaacggct
      901 tcgagtgcga tgcggcattc accacctgca cgaccaccgg gatcgtcgcg agcttgccgt
      961 tgcgatcggt ggaggagagc gcccggtcgc ggaccgggtg cttcatcttg ccctcctcga
     1021 actgatacgc cttcgcgcgg agcatgagat gcgggaagtc cacgttccac ggatgcggcg
     1081 gaacgtacgg gcatttcacg acgtagcaga ggtcgcacag gtagcactgg tcgaccactt
     1141 cccagtactt ctccttcgga acgccgtcga tctcgccgcc ggggccggcg tcgatgaagt
     1201 cgaagagctt cggaaacgtg ttgcagaggc tcacgcagcg ccggcagccg tggcagatct
     1261 cgaagacgcg ctcggcctcg gcgaggatct ttccctcgtc gcgatactcc gggctcttcc
     1321 agtcgatcgg atggcgcgtg ggcgcctcga gactgccttc gcgctgggcc atcaaatcaa
     1381 tcggtgagct ggtccagcgc cttctggaag cggttggcgt gcgagcgctc ggccttcgcg
     1441 agggtctcga accagtccgc gatttcatcg aagccttccg tgcgcgccga tttggccatg
     1501 cccgggtaca tgtcggtgta ctcgtgggtc tcgccggcga tcgcggcctt caggttctgg
     1561 cgcgagctgc cgatgggcag gtcggtcgcc gggtcgccga ccgcggcgag gtagtcgagg
     1621 tggccgtgcg cgtggccggt ctcgccttcg gccgtggagc ggaagatcgc cgagacgtcg
     1681 ttctggcctt cgacgtcggc cttgttcgcg aaatagaggt agcggcgatt cgcctgggat
     1741 tcaccggcga acgcggcctt caggttgtcg tgggtcttcg tgcctttgag tggtttcatc
     1801 ggtgcctcca gggtggaaat ggtgcggatt cacaagggtg ctgctttgcc actcgagagt
     1861 attgcgcgcg cgcaacaacc gtccaattga ttgttccgat gcgcgcgatt ggggatgcct
     1921 atcgcgctcc gcgcagccgc ttgagtgcgg cgcgatacgc ttccgcggcg gcctcctgcc
     1981 gcgagtgcac gccctgggac accaccgccg tgccgcc
//
LOCUS       DASVII010000015         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_396300,
            whole genome shotgun sequence.
ACCESSION   DASVII010000015 DASVII010000000
VERSION     DASVII010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396300"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..877
                     /locus_tag="VF309_00180"
     CDS             308..877
                     /locus_tag="VF309_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7465024.1"
                     /translation="MKAPSPKPTGKPALTDVKTLRESARRHVEDGAVTEHYKGDRQKV
                     LKLLNAALATEIICVLRYRHDYFTARGLKAKAAAAEFLEHANEELGHADQLAERIVQL
                     GGEPDFNPQTLTERSHAEYRLGRTIEEMIKENLVAERIAIDSYRQMAAYIGDDDPTTK
                     NLLEEILAVEEEHADDLADLLDHKAGTKG"
     gene            complement(938..1168)
                     /locus_tag="VF309_00185"
     CDS             complement(938..1168)
                     /locus_tag="VF309_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7465025.1"
                     /translation="MNTDQMHGHWREVKGWALEHLGRVTNDPWKRIAGRRERLLGRLE
                     ASLAESRRMAEATFPAVHATAASGERRRAAGR"
     gene            complement(1188..1427)
                     /locus_tag="VF309_00190"
     CDS             complement(1188..1427)
                     /locus_tag="VF309_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465026.1"
                     /translation="MKNILAIGLACCIPLGLAAEDPSEAYLGTVNVLVIEDAAPGHQM
                     CAGPAERSECMAFSRAVLTARVQKVSALHERGRWI"
BASE COUNT          303 a          520 c          523 g          252 t
ORIGIN      
        1 cggtcgaagg catcgagtcc caggccgcgg cgctgctcgc catcgaggcc aaggccgact
       61 acgtgcaggg ctatttcttc gcgagcccgc agtctcgtct caccgacgag atcggcggca
      121 cggcgctcct ggaccacctg ctgcattccc aggacggccc gcgcctggcc gcctgagggc
      181 acgttcttcc cggcaagaaa ttcacggcct ttcgcacgaa ccctttgcgg gcgggctgtt
      241 ccaatgggag gtaggccgcg cgggaatcgc acggccgttg cgcatcgacc ctccaaggag
      301 aacaacattg aaagccccca gccccaagcc cacgggaaag cccgctctta ccgacgttaa
      361 gaccttgcgc gagtccgcgc ggcgccacgt cgaggacggc gcggtcaccg agcactacaa
      421 gggggatcgg cagaaggtgc tgaaactgct gaacgcggcg ctcgcgacgg agatcatctg
      481 tgtcctgcgc taccgccacg actatttcac cgcccgcggc ctgaaggcca aggcggcggc
      541 cgcggaattc ctcgagcatg ccaacgagga gctgggccat gccgaccagc tggcggagcg
      601 catcgtgcag ctgggcggcg agccggactt caacccgcag acgctcaccg agcgcagcca
      661 cgccgagtac cgcctcggca ggaccatcga ggagatgatc aaggagaacc tggtcgccga
      721 gaggatcgcg atcgacagct atcggcagat ggcggcctat atcggcgatg acgatccgac
      781 caccaagaac ctgctcgagg agatcctcgc ggtggaggaa gagcacgccg acgacctcgc
      841 cgacctcctc gaccacaagg ccggcaccaa ggggtaggcg aaaaaaaacc tgcggaccca
      901 ggaaacgaaa gtccgcaggc ttctcagcga atcgttgtca gcgtccggcg gccctgcggc
      961 gttcgccgga tgcagcggtc gcgtgcacgg ccgggaaagt ggcctcggcc atgcgccgcg
     1021 attccgcgag cgaggcctcg agccggccca ggagacgctc gcgccttccg gcgatgcgct
     1081 tccagggatc gttggtcacg cgcccgaggt gctcgagggc ccaacccttc acttcgcgcc
     1141 aatggccgtg catctggtcg gtattcatcg tggctctccg taagtggcta gatccaacgg
     1201 ccgcgctcgt ggagcgcgga aactttctgg actcgggcgg tcaataccgc acggctgaac
     1261 gccatgcatt cggagcgctc cgccgggccg gcgcacatct ggtggccggg cgcggcatcc
     1321 tcgatcacga ggacattcac cgttccgagg taggcctcgg acggatcttc cgcggcgagc
     1381 cccagcggta tgcagcaggc gaggccgatt gcgaggatat ttttcatggg ccaactccct
     1441 tggtgttcgt tcatagtgaa cagggcaccc ggaaggggga gtcagtgggc ggaggttcgc
     1501 gcctaggact cgtctgatgt tccggtcaga ggattcctac agcggctatt ccgttgatcg
     1561 gcaagaaaaa aagcccgcgg atgcgggctt tttctcgt
//
LOCUS       DASVII010000016         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_576128,
            whole genome shotgun sequence.
ACCESSION   DASVII010000016 DASVII010000000
VERSION     DASVII010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576128"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF309_00195"
     CDS             complement(<1..331)
                     /locus_tag="VF309_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465027.1"
                     /translation="MRPWQSDPLLKVEHLTMRFGGLTAVDDVSFEVGRNDITALIGPN
                     GAGKTTVFNCITGFYKPTEGSMELRHPGGETFRLERLDDHNINSDARVARTFQNIRLF
                     RGMTVLEN"
     gene            complement(328..>1558)
                     /gene="livM"
                     /locus_tag="VF309_00200"
     CDS             complement(328..>1558)
                     /gene="livM"
                     /locus_tag="VF309_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEX7465028.1"
                     /translation="AVLVLIVAAIMGAPAYQLVAGLVLGFLAIGVIRRVIVFFFPDLP
                     YARIFTIAGILFAIFFPLSSYLLEKAFHLGLPLRYLFDLSILVLTYVMLGWGLNIVVG
                     LAGLLDLGYVAFYAVGAYVFALLATHFGLGFWTCLPIAGAAAALWGIVLGFPVLRLRG
                     DYLAIVTLAFGEIVRIVLLNWTVVTNGPNGISGIPKPTFFGLPFTATGPETFASFFSL
                     KYDPIQGIIFLYYVILVLALITNLVTLRLRRLPIGRAWEAMREDEIACRSLGINTTTT
                     KLTAFALGAMFGGFAGSFFATRQGFISPESFTFIESAIILAIVVLGGMGSQLGVAIAA
                     IVMVGGFEALRHTDWLQDIFGQEFDPALYRMLLFGIAMVAIMVWRPRGIVGSRTPSIV
                     LEKAKAISSAHVEEGRA"
BASE COUNT          323 a          433 c          571 g          231 t
ORIGIN      
        1 aattctcgag cacggtcatg ccacggaaca gcctgatgtt ctggaaggtg cgggcgacgc
       61 gggcgtcgga gttgatgttg tggtcgtcga gccgctcgag ccggaaggtc tcgccgccgg
      121 ggtgacgcag ctccatgctc ccctcggtcg gcttgtagaa gccggtgatg cagttgaaca
      181 cagtcgtctt gccggcgccg ttcgggccga tcagggcggt gatgtcgttg cgtccgacct
      241 cgaaggagac gtcgtcgacg gcggtcagcc cgccgaagcg catggtgagg tgctcgacct
      301 tgagcagggg gtcgctctgc cagggcctca tgcgcggcct tcttcgacgt gagctgagga
      361 tatggctttg gctttttcga gcacgatcga gggcgttcgc gagccgacga tgccgcgcgg
      421 ccgccatacc atgatcgcca ccatggcgat gccgaacagc agcatgcggt agagcgcggg
      481 gtcgaactcc tggccgaaga tgtcctggag ccagtcggtg tggcggagcg cctcgaagcc
      541 gccgaccatg acgatcgcgg cgatcgcgac gccgagctgg ctgcccatgc cgccgagcac
      601 gacgatggcg aggatgattg ccgattcgat gaaagtgaag ctctcagggc tgatgaaacc
      661 ctgtcgggtg gcgaagaacg agcccgcgaa gccgccgaac atcgcgccga gggcgaaggc
      721 ggtaagcttg gtcgtggtgg tgttgatgcc gagcgagcgg caggcgatct cgtcctcgcg
      781 catcgcttcc caggcgcgcc ctatcggcag ccgccgcaat ctaagcgtca ccagattggt
      841 gatgagcgcc aggacgagga tcacgtaata gaggaagatg atgccctgga tgggatcgta
      901 tttgaggctg aagaagctgg cgaaggtctc aggccccgtc gccgtgaacg gcaggccgaa
      961 gaaagtcggc ttgggaatgc cggatatgcc gttgggcccg ttggtgacca cggtccagtt
     1021 gagcaggacg atgcgcacga tttcgccgaa ggcgagcgtg acgatggcga gatagtcgcc
     1081 gcgaagccgg agcacgggaa agccgagaac gatgccccac agcgccgcgg cggctccggc
     1141 gatcggcagg catgtccaga agccgaggcc gaaatgcgtg gcgagcagcg cgaacacata
     1201 ggcgccgacg gcgtagaagg cgacatagcc gaggtcgagc aggccggcga ggccgaccac
     1261 gatgttgagg ccccagccga gcatcacata ggtgagcacg aggatcgaca ggtcgaacag
     1321 atagcggagc ggcaggccga gatggaacgc cttctccaga aggtaggacg agagcgggaa
     1381 gaagattgcg aacaggatgc cggcgatggt gaagatgcgc gcgtaaggaa ggtcggggaa
     1441 gaaaaatacg atcacgcggc ggatgacgcc gatggcgagg aacccgagca cgaggccagc
     1501 gaccagctga taggccggcg cgcccatgat cgcggcgacg atgagcacga gcacggcg
//
LOCUS       DASVII010000017         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_540096,
            whole genome shotgun sequence.
ACCESSION   DASVII010000017 DASVII010000000
VERSION     DASVII010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540096"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF309_00205"
     CDS             complement(<1..190)
                     /locus_tag="VF309_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465029.1"
                     /translation="MNLRDAEPQLAPRKVGEVILAVEDVSLAFGGVKALSEVGFDVRE
                     HEVRAIIGPNGAGKSSMLN"
     gene            complement(187..2145)
                     /locus_tag="VF309_00210"
     CDS             complement(187..2145)
                     /locus_tag="VF309_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7465030.1"
                     /translation="MTLESGNRDTFPKLLLGHALERGDHPAIREKALGIWQSWSWAEV
                     RDEIEWLACGLAQAGLARGDHIAVIGANRPRLYWTLTAAQSLGAIPVPFYEDAVAQEM
                     VYVFQDADIAFAVVEDQEQADKLFEIMPQCPKLKRIWFDDPRGLRKYAQEELASYESL
                     RETGKAFAGSHDGFFAAEVARGAGPDTAIMLYTSGTTGHPKGVVLSHDNLIHASRAYA
                     ELEGLRDDEEVLAYLPMAWIGQNLFSYAQWMVVGFRINCIESAETVVNDMREIGPTYY
                     FAPPRVLEALLTQVTIRMEDASWVKRRLFAYFMDLARRVGSNLLDARPVSLVDRMKYA
                     IGNILMYAPLKNSLGMSRVRVAYTAGEAIGPDLFVFYRSLGINLKQLYGSTETSVMVC
                     VQPNGEVRPDTVGPPMKGVEVRVLDSGEIVLRCPGLFRAYYKNDAATREAKDADGWFH
                     TGDAGFFVADGHLKIIDRAKDVGKLSDGTLFAPKYLENKLKFFPYVKEAVAFGDGRAQ
                     ATVMVNIDMPAVGDWAERRGIPYSGYTDLAAKAQVTELVRECVEKVNADLARDPKLCA
                     SQIHRFVILHKELDADDGELTRTRKVRRGFIGEKYAKIVEALYSGADRCVVEAQVRFE
                     DGRTGKVRAELHVNPAKTFAPERVLAAA"
     gene            complement(2198..>2774)
                     /locus_tag="VF309_00215"
     CDS             complement(2198..>2774)
                     /locus_tag="VF309_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7465031.1"
                     /translation="FDDTIAAGQSFEGGGLEWEAHGAPGHDMDALMFHEPVNRVLISG
                     DALWKDGMGFVWPEEGPNPFIEAARESLATIERLDPALVIPGHGAPFADVAGAIARVR
                     ERLDAFERDPAKNARHVVKVLFVFALLERREMSVADVAGYVGRVPCYRQMSARFLGLD
                     DAALAAYLLADLERAGAIAIREGIARPTMAA"
BASE COUNT          433 a          951 c          921 g          469 t
ORIGIN      
        1 cgttaagcat cgagctcttg ccggcgccgt tgggcccgat gatggcgcgc acctcgtgct
       61 cgcgcacgtc gaagcccact tccgagagcg ccttcacccc gccgaacgcg agtgacacgt
      121 cctcgaccgc gaggatcacc tcgcccacct tgcgcggcgc gagttgcggc tcggcgtccc
      181 gcaggttcat gcggccgcca ggacccgttc cggcgcgaag gtcttcgccg gattgacgtg
      241 caactccgcg cggaccttgc cggtgcgccc gtcctcgaag cgcacttgcg cctcgacgac
      301 gcagcgatcc gcgccggaat agagcgcttc gacgatcttc gcgtacttct cgccgatgaa
      361 gccgcggcgc accttgcgcg tgcgcgtgag ctcgccgtcg tcggcgtcga gctccttgtg
      421 caggatcacg aaacggtgga tctggctcgc gcagagcttg ggatcccgcg cgaggtccgc
      481 gttgaccttt tcgacgcact cgcgcaccag ctcggtcacc tgcgccttcg ccgcgaggtc
      541 cgtatagccg gaatagggaa tcccgcggcg ctcggcccag tcgcccacgg cgggcatgtc
      601 gatgttcacc atcaccgtcg cctgggccct gccgtccccg aaggcgacgg cttccttgac
      661 gtacgggaag aacttgagct tgttctcgag atacttcggc gcgaagagcg tgccgtcgga
      721 gagcttgccg acatccttgg cgcgatcgat gatcttgaga tgcccgtccg cgacgaagaa
      781 gccggcgtcg cccgtgtgga accagccgtc ggcgtccttc gcttcgcgcg tggcggcgtc
      841 gttcttgtaa taggcgcgaa acaggcccgg gcagcgcagc acgatctcgc ccgaatcgag
      901 cacgcgcacc tcgacgccct tcatcggcgg gccgaccgtg tcgggcctca cctcgccgtt
      961 gggctgcacg cacaccatca ccgaggtctc ggtcgatccg tagagttgct tgagattgat
     1021 ccctagcgag cgatagaaga cgaacagatc cggcccgatc gcctcgcccg cggtgtacgc
     1081 gacgcgcacg cgcgacatgc cgagggaatt cttcagcggc gcgtacatca ggatgttgcc
     1141 gatggcgtac ttcatccggt cgaccaggct cacggggcga gcgtcgagga ggttcgagcc
     1201 gacgcggcgc gcgaggtcca tgaagtaggc gaagaggcgc cgcttcaccc agctcgcgtc
     1261 ctccatgcgg atcgtcacct gcgtgaggag cgcctcgagg actcgcggcg gcgcgaagta
     1321 gtacgtcggg ccgatctcgc gcatgtcgtt gacgacggtc tcggcggatt cgatgcagtt
     1381 gatgcgaaag ccgaccacca tccactgggc gtaggaaaac aggttctgcc cgatccacgc
     1441 catcgggaga taggcgagca cctcctcgtc gtcgcgcaac ccctcgagct cggcgtaggc
     1501 gcggctcgcg tggatgagat tgtcgtggga gaggaccacg cccttgggat ggcccgtggt
     1561 tcccgacgtg taaagcatga tcgcggtatc ggggccggcg ccccgggcca cctcggcggc
     1621 gaagaatccg tcatgggacc ccgcgaacgc ctttccggtc tcgcgaaggc tctcgtagct
     1681 cgcgagctcc tcctgggcgt atttgcgcaa tccgcgtgga tcgtcgaacc agatgcgctt
     1741 gagcttcggg cattgcggca tgatctcgaa gagcttgtcg gcctgctcct ggtcctccac
     1801 gaccgcgaac gcgatatccg cgtcctggaa gacgtagacc atctcctgcg cgacggcgtc
     1861 ctcgtagaac ggaaccggaa tggcgccgag ggactgcgcc gcggtgagag tccagtagag
     1921 cctcgggcgg ttcgcgccga tgaccgcgat gtggtcgccg cgagcgaggc ccgcctgcgc
     1981 gagcccgcat gcgagccact cgatctcgtc gcgcacttcg gcccagctcc agctctgcca
     2041 gatgcccagc gccttctcgc gaatggccgg gtgatcgccc cgctccagcg cgtggcccag
     2101 aagcagcttc gggaacgtgt cgcggttccc gctttccagc gtcatggctg gctcctctcg
     2161 ttgtgcgcct agtgtagccg gaaccgctgc ggttccctca cgcggccatg gtcgggcgcg
     2221 cgatgccctc gcgaatcgcg atggccccgg cccgctcgag gtcggcgagc agataggcgg
     2281 cgagcgcggc gtcgtcgagc ccgaggaaac gcgccgacat ctgccggtag cagggcacgc
     2341 ggccgacgta gcccgcgacg tcggcgacgc tcatttcccg ccgctcgagg agcgcgaaga
     2401 cgaacagcac cttcacgacg tggcgcgcgt tcttcgcggg atcgcgctcg aaggcgtcga
     2461 ggcgctcgcg cacgcgtgcg atcgcgccgg ccacatccgc gaagggcgcc ccgtgcccgg
     2521 gaatgacgag cgcggggtcg agcctttcga tggtcgccag ggattcgcgc gcggcctcga
     2581 tgaaggggtt cgggccctct tccggccaga cgaatcccat gccgtctttc cagagcgcat
     2641 cgcccgagat gagcacgcga ttcaccggct cgtggaacat gagcgcatcc atgtcgtggc
     2701 cgggcgcccc gtgggcctcc cactcgaggc cgccgccttc gaacgattgt cccgccgcga
     2761 tcgtgtcgtc gaaa
//
LOCUS       DASVII010000018         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_576201,
            whole genome shotgun sequence.
ACCESSION   DASVII010000018 DASVII010000000
VERSION     DASVII010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576201"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VF309_00220"
     CDS             complement(<1..875)
                     /locus_tag="VF309_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465032.1"
                     /translation="MQKLRPLVHCVAGAFATAALAALPGAALAATSLTIYSSAQPGTL
                     DPRTFMSGGESGIVPGYATVREERSFDFKAGRNLLRVSDVPALIDPTTVAWVSLRDPK
                     GTRVVEQNFEFDLTSTAKLLSRYIDRDITVEQSRGPQLEAITGTLVSTQGGLVLKQPD
                     GSVRIVSEHSGVRLPGIPGGLISKPTLAWDIDASTPGTQAARFSYQTGGMTWWTDYNL
                     TYSEPKPGACRVDVAAWVTIVNQSGAAFADASVKLMAGDVQRARPRAYGMVDQAMAAA
                     PALRKAEGFQEKAFF"
     gene            957..1799
                     /locus_tag="VF309_00225"
     CDS             957..1799
                     /locus_tag="VF309_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7465033.1"
                     /translation="MKFQGTETYVADDDLKLAVNAAITLERPLLIKGEPGTGKTMLAE
                     EVARALGKPLYQWHIKSTTKAQQGLYEYDAVSRLRDSQLGNERVGDIANYILKGVLWQ
                     AFHSPEQAVVLVDEIDKADIEFPNDLLRELDRMEFYVYETRETVRAIHRPIIFITSNN
                     EKELPDAFLRRCFFHYIRFPDGDTMQAIVDVHFPGIKKKLLQAALEVFFEIREMPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRTPGHQASIPPLYGALLKNEQDVHLFEQLIFLQ
                     RRGR"
     gene            1803..>2032
                     /locus_tag="VF309_00230"
     CDS             1803..>2032
                     /locus_tag="VF309_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465034.1"
                     /translation="MATRKAPARKSATRRPPRRKPTATKVAIRKPSVRKAPAKRPKSS
                     PPGRAARASRAAKGMRYAGVGSEAVFRATGRA"
BASE COUNT          344 a          688 c          693 g          307 t
ORIGIN      
        1 tcgaagaagg ccttctcctg gaaaccttcg gccttcctca gggcgggcgc cgccgccatg
       61 gcctgatcca ccatgccgta agcgcgcggg cgggcgcgct gcacgtctcc cgccatgagc
      121 ttcacggacg cgtccgcgaa agccgcgccc gactggttca cgatcgtcac ccacgcggcc
      181 acgtcgacgc ggcacgcgcc gggcttcggc tccgaatacg tgaggttgta gtcggtccac
      241 cacgtcatgc cgcccgtctg gtagctgaac cgcgccgctt gcgttccggg cgtagacgcg
      301 tcgatgtccc aggcgagcgt gggcttgctg atgaggcctc cgggaatccc cggaagcctt
      361 acgcccgagt gctcgctcac gatgcgcacg ctgccgtcgg gctgcttcag cacgaggccg
      421 ccctgggtcg agacgagcgt gcccgtgatc gcttccagct gcgggccgcg cgactgctcg
      481 acggtgatgt cgcggtcgat gtagcgcgac agcagcttcg cggtgctggt gaggtcgaac
      541 tcgaagttct gctcgaccac gcgcgtgccc ttcgggtcgc gaagcgacac ccacgcgacc
      601 gtggtcgggt cgatgagggc cgggacgtcg ctcacgcgca gcaggttgcg gcccgccttg
      661 aagtcgaagg agcgctcctc gcgcaccgtc gcgtatccgg gcacgatgcc ggactctccg
      721 ccgctcatga aggtgcgcgg atcgagcgtt ccgggctggg cgctcgagta gatggtgagc
      781 gaggtcgccg cgagcgcggc gccgggaagg gcggcgaggg ccgcggtcgc gaatgcgcct
      841 gcgacacaat ggacgagcgg gcgaagcttc tgcatgggac acctccgttg tgggtctgga
      901 ggtgtaaacg tgagccgcgg caaaaagggt cgaatcgccg cgggtataat ttcgggatga
      961 agttccaggg caccgaaacc tacgtcgccg acgacgacct caagctcgcg gtgaacgccg
     1021 cgatcacgct cgagcgcccc ctgctcatca agggagagcc cggaaccggc aagacgatgc
     1081 tcgccgagga ggtggcccgc gccctgggca agcccctgta ccagtggcac atcaagtcga
     1141 ccaccaaggc gcagcagggc ctctacgaat acgacgccgt ttcgcgcctt cgggattcgc
     1201 agctcggcaa cgagcgcgtg ggcgacatcg ccaactacat attgaagggc gtcctgtggc
     1261 aggcgttcca ttcgcccgag caggccgtgg tcctcgtcga cgagatcgac aaggccgaca
     1321 tcgagtttcc gaacgacctc ctgcgcgagc tcgaccgcat ggagttctac gtctacgaga
     1381 cgcgggagac ggtgcgcgcg atccaccggc cgatcatctt catcacgtcg aacaacgaga
     1441 aggagctccc ggacgccttc ctgcggcgct gcttcttcca ctacatccgc tttcccgacg
     1501 gggacacgat gcaggcgatc gtcgacgtcc acttcccggg aatcaagaag aagctgctgc
     1561 aggcggcgct cgaggtgttc ttcgagatcc gcgagatgcc cggcctgaag aagaagccct
     1621 cgacctcgga gctcctcgat tggctgaagc tgctgctcgc cgaggacatc ccgcccgagg
     1681 cgctgcgcac gcccgggcac caggcgtcga ttccgccgct gtacggggcg ctcctcaaga
     1741 acgagcagga cgtgcacctg ttcgagcagc tgatctttct gcagcgtcgc gggaggtagc
     1801 cgatggcgac tcgcaaggcc ccggcgcgca agagcgccac tcgccgcccg ccgcggcgca
     1861 agcccaccgc gaccaaggtg gcgatccgca agccgagcgt gcgcaaggct cccgcgaagc
     1921 gcccgaagtc gagcccgccg gggcgcgcgg cgcgcgcgag ccgcgccgcc aagggcatgc
     1981 gctacgccgg cgtcggcagc gaagcggtgt ttcgagcgac gggccgcgcg tg
//
LOCUS       DASVII010000019         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_432393,
            whole genome shotgun sequence.
ACCESSION   DASVII010000019 DASVII010000000
VERSION     DASVII010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432393"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1432
                     /locus_tag="VF309_00235"
     CDS             95..1432
                     /locus_tag="VF309_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7465035.1"
                     /translation="MTILSDQLQLAPAELDSYWMPFTANRQFKAAPRMVGKAEGMYYW
                     TSDGRKVLDGFAGLWCCNAGHCRKPIVEAIQKQAATMDYAPAFQMSHPLAFELANRIV
                     ELAPGKLDHVFFCNSGSEAVDTALKIAIAWHRVRGEGSRTRLIGRERGYHGVGFGGIS
                     VGGIPANRKMFGTMLAGVDHLQHTHNLEKNAYSKGQPEWGAQLADELERLVVLHDAST
                     IAAVIVEPVAGSTGVLIPPKGYLQRLREITKKHGILLIFDEVITAFGRLGAAFGADVY
                     GVEPDMITFAKGVTSGTVPMGGVLVSSDIYHAFMSGPPGAIELFHGYTYSAHPLACAA
                     GIATLQLYKDEDLFARAARMAPAFERALHSLKGTRHVVDIRNQGLVGGIELAPRAGSP
                     GARGFEAYLRVYEKNVLVRSTGDILAVAPALIVEEAHLDQIVSTIREVLTALE"
     gene            1479..>2069
                     /locus_tag="VF309_00240"
     CDS             1479..>2069
                     /locus_tag="VF309_00240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7465036.1"
                     /translation="MDAKGKVALVTGASAGIGKASALALLREGYRVVLTARRRELLDQ
                     AIAESGAGADRALAVTSDVGDPKSVASLFAAVKEKFGRLDVLFNNAGMGAPAVPMEDL
                     PFEKWQAVVSANLTGTFLCCQEAVRIMKAQDPKGGRIINNGSISAHAPRPMSIAYTAT
                     KHAITGLTKSTSLDGRQYDIACGQIDIGNALTEMTAR"
BASE COUNT          346 a          713 c          689 g          321 t
ORIGIN      
        1 cactgccgga tgacgcccat tcgcaagtca gcatcgtccg acgcggttgt aggcgattgc
       61 ggtagattgg aggtctccga gcgaggattc ccccatgacg atactttccg accagctgca
      121 gctcgcgccc gccgagctcg attcgtactg gatgcccttc accgcgaacc gccagttcaa
      181 ggccgcgccg cgaatggtcg gcaaggccga aggcatgtat tactggacca gcgatggccg
      241 caaggtcctc gacggcttcg ccgggctgtg gtgctgcaac gccggccatt gccgcaagcc
      301 catcgtggaa gcgatccaga agcaggccgc tacgatggac tacgcgcccg ctttccagat
      361 gagccacccg ctcgccttcg agctcgccaa ccgcatcgtc gagctcgcgc ccggcaagct
      421 cgatcacgtc ttcttctgca actcgggctc cgaagcggtg gacacggcgt tgaagatcgc
      481 gatcgcatgg caccgcgttc gcggcgaggg ctcgcgcacg cgcctcatcg gccgcgagcg
      541 cggctaccac ggcgtggggt tcggcggcat ctccgtcggc ggcattcccg cgaaccgcaa
      601 gatgttcggc acgatgctcg cgggcgtgga ccacctgcag cacacgcaca acctcgagaa
      661 gaacgcgtat tcgaaaggcc agcccgagtg gggcgcgcag ctcgccgacg agctcgagcg
      721 cctggtggtg ttgcacgacg cctcgaccat cgcggcggtg atcgtcgagc ccgtcgcggg
      781 atccacgggc gtgctgatcc cgccgaaggg ctacctgcag cggctgcgcg agatcacgaa
      841 gaagcacggc atcctcctca tcttcgacga ggtgatcacc gccttcggcc gcctcggggc
      901 ggccttcggc gcggatgtgt acggcgtcga gcccgacatg atcacgttcg cgaagggcgt
      961 gacctcgggc accgtgccca tgggcggcgt gcttgtctcg tccgacatct atcacgcgtt
     1021 catgtcgggc ccgcccggag cgatcgagct cttccacgga tacacctatt ccgcgcatcc
     1081 gctcgcgtgc gcggccggaa tcgcgacgct gcagctctac aaggacgagg atctcttcgc
     1141 ccgcgcggcg cggatggcgc ccgcgttcga gcgcgcgctg cattcgctga agggcacgcg
     1201 ccacgtcgtg gacatccgca accagggcct cgtgggcggc atcgagctcg cgccgcgcgc
     1261 gggctcgccg ggcgcgcgcg gcttcgaggc gtaccttcgc gtctacgaga agaacgtgct
     1321 ggtgcgctcg accggcgaca tcctcgccgt cgcccccgcg ctcatcgtcg aggaggcgca
     1381 cctcgaccag atcgtctcga cgattcgcga ggtcctcacc gcgctcgagt gaatcgggga
     1441 taatcgccgc tttccgcaca cagcgaaggg gaaggcccat ggacgcaaag gggaaagtcg
     1501 cgctcgtcac cggcgcgagc gccggcatcg gcaaggcctc ggcgctcgcg ttgctgcgcg
     1561 agggctatcg cgtggtgctc acggcgcgcc gccgcgagct cctcgaccag gcgatcgccg
     1621 aatcgggcgc gggagcggac cgggcgctcg cggtcacgtc cgacgtgggc gatcccaaat
     1681 ccgtcgcctc gctcttcgcg gcggtgaagg agaaattcgg ccgcctcgac gtgctcttca
     1741 acaatgccgg catgggcgcg cccgcggtgc cgatggagga cctgccgttc gagaaatggc
     1801 aggcggtcgt ctcggcgaac ctcacgggaa cgtttctctg ctgccaggag gcggtgcgca
     1861 tcatgaaggc gcaggacccg aagggcggac gcatcatcaa caacggctcc atctccgccc
     1921 atgcgccgcg cccgatgtcc atcgcgtaca cggcgaccaa gcacgcgatc acggggctca
     1981 ccaagtcgac gtcgctcgat ggcaggcagt acgacatcgc gtgcggccag atcgacatcg
     2041 ggaacgcgct gaccgagatg acagcgcgg
//
LOCUS       DASVII010000020         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_432666,
            whole genome shotgun sequence.
ACCESSION   DASVII010000020 DASVII010000000
VERSION     DASVII010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432666"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..423)
                     /gene="hemJ"
                     /locus_tag="VF309_00245"
     CDS             complement(10..423)
                     /gene="hemJ"
                     /locus_tag="VF309_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEX7465037.1"
                     /translation="MLWLKALHIIFVVAWFAGLFYLPRLFVYHAQSSDAASNERFKVM
                     ERKLYRGIMTPCMVLTLVLGSWMWLGYDFHGGWLRAKLVLVGLLVGYHFWLGRLASDF
                     AADANRRSHVFYRWINELPLLLLAAIVILVVVKPF"
     gene            443..2584
                     /locus_tag="VF309_00250"
     CDS             443..2584
                     /locus_tag="VF309_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX7465038.1"
                     /translation="MFTLSPVLAAAFAAIALLASPATRAATLVKTEHVEADLVASKAG
                     AQPGKPLLVGLRLRMEPQWHTYWRNPGDSGLPTKIQWVLPEGWKAGEIQWPFPQPLPV
                     GTLMNYGYEDEVVLLAELTPPASAPAGNAAIKARADWLVCKDICVPEKGELDFVLPVA
                     SRDAAADPRFVGSVERALGMLPVDAAGWKYEAALQGKSLVVRLTPPEGAVAPAKATFF
                     PERESLIEPAAPQRLTREGRALRIEMKLADPPIAGMTSVKGVAVSDSGWNGSSGRKAI
                     NVAAPLGAPLGAPAASTSTDAPAGGGMFAALAFAFLGGILLNLMPCVFPVLGIKVLGF
                     VEHAHGDARAMRLQGAIFFAGVVTSFLALAAIMLGLRAGGTQLGWGFQLQSPVVVAFL
                     AALFFVLALNLSGVFEWGAFAQSVGARLSARGRYADAFLSGALATVVATPCTAPFMGA
                     AVGFTLTQNAALSLAIFATLGAGMALPVLLLSFFPALLKRLPKPGAWMETFKQVLAFP
                     LYATVAWLAWVLGAQSGNDAVFALLAGLVMVAMGAWIYGRSLHAEGIWRPTLAAILVA
                     GGLAVAWPGGAMDASGSAAAATDDLQWQAWSPEKVRALTAAGRPVFVDFTAAWCVTCQ
                     VNKRVALHNASVIGAFAAHDVATLKADWTRQDPRITATLAALGRNAVPVYALYLPGED
                     APRLLPEVLTPALVLAEIEKLPAAKTAVTQR"
     gene            2601..3209
                     /locus_tag="VF309_00255"
     CDS             2601..3209
                     /locus_tag="VF309_00255"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEX7465039.1"
                     /translation="MRPLKGKTMRHASIVILAAVTLFISGCATMSAQTPGAGLSPVNA
                     IRDGGDEHVILFGHDVVSYFTEGTHRMGLPSIKSEYKSVTFRFSKPENKALFDAAPEK
                     YIPQFGAYCTNGIVYAIPAGGNPDTWKIIDGKLYIFGGQGALDGFMLDVPRNMALAHK
                     YWAEEVNGSNPFVQRYKRWIFRVPHYVTGKELAEQVAKAKKG"
BASE COUNT          525 a         1131 c         1111 g          545 t
ORIGIN      
        1 gaggtcattt cagaagggct tcaccacgac gaggatgacg atggcggcaa gcagcaggag
       61 gggcagctcg ttgatccagc ggtagaagac gtggctgcgg cgattggcgt cggccgcgaa
      121 gtcgcttgcg agccgcccca gccagaagtg ataaccgacg aggaggccca cgaggacgag
      181 cttggcgcgg agccatcccc cgtgaaaatc gtagccgagc cacatccagc tccccagcac
      241 gagcgtaagc accatgcagg gcgtcatgat gccgcggtag agcttgcgct ccatcacctt
      301 gaagcgctcg ttggaagcgg catccgatga ttgggcgtga tagacgaaga ggcgcggcag
      361 gtagaacagg cccgcgaacc acgcgaccac gaagatgatg tgcaacgcct tgagccagag
      421 cataagacta taattttacg tgatgttcac actctctccc gttctcgccg cggctttcgc
      481 cgcgatcgcg ctcctcgcct cgcccgccac gcgcgcggcg accctggtga agaccgagca
      541 tgtcgaggcc gatctcgtcg cttccaaggc gggcgctcaa cccggcaagc cgctcctcgt
      601 gggtttgcgc ctgcgcatgg agccgcagtg gcacacgtac tggagaaatc ccggcgattc
      661 gggactgccg acgaagatcc agtgggtcct tcccgagggc tggaaggcgg gcgagatcca
      721 gtggccgttc ccgcagcccc tgcccgtggg cacgctcatg aactacggct acgaggacga
      781 ggtggtgctc ctcgcggagc tgacgccgcc cgcctcggcg ccggcgggca acgccgccat
      841 caaggcgcgc gccgattggc tcgtctgcaa ggacatctgc gttcccgaga agggcgagct
      901 cgatttcgtc ctgcccgtgg cgagccgcga tgccgccgcc gatccgcgtt tcgtcggaag
      961 cgtcgagcgc gcgctcggca tgctgcccgt cgacgcggcg ggatggaagt acgaggccgc
     1021 gttgcagggc aagtcgctcg tcgtgcgcct cacgcccccc gaaggcgcgg tcgcgcccgc
     1081 gaaggcgacg ttctttcccg agcgcgagag cctcatcgag cccgcggcgc cgcagaggct
     1141 cacgcgcgaa gggcgcgcgc tgcgcatcga gatgaagctc gccgatccgc ccatcgcggg
     1201 aatgacctcg gtgaagggcg tggcggtatc cgacagcggc tggaacggct cgagcggccg
     1261 caaggcgatc aacgtcgcgg cgccgctggg cgcgccgctg ggcgcgccgg cggcaagcac
     1321 ttcgacggat gcacccgccg gtggcggcat gttcgccgcg ttggccttcg cgttcctcgg
     1381 cggcattctc ctgaacctca tgccgtgcgt gttccccgtg ctggggatca aggtgctcgg
     1441 cttcgtcgag catgcccatg gcgatgcgcg cgcgatgcgc ctgcaagggg cgatcttctt
     1501 cgcgggcgtg gtgacgtcgt tcctcgcgct cgcggcgatc atgctcgggc tgcgcgcggg
     1561 cggcacgcag ctcgggtggg gattccagct gcaatcgccc gtcgtcgtgg cctttctcgc
     1621 ggcgctcttc ttcgtgctcg cgctgaacct ctcgggcgtc ttcgaatggg gcgccttcgc
     1681 ccagtcggtg ggcgcgcggt tgagcgcgcg cggccgctat gcggacgcgt tcctctcggg
     1741 cgcgctcgcg accgtcgtcg ccacgccgtg caccgcgcct ttcatgggcg ccgccgtggg
     1801 cttcacgctc acgcaaaatg cggcgctctc gctcgcgatc ttcgcgacgc tgggcgcggg
     1861 catggccctt ccggtgctcc tgctctcgtt ctttccggcg ctgctgaagc ggctgccgaa
     1921 gcccggcgcg tggatggaga ccttcaagca ggtgctcgcg ttcccgctct atgcgacggt
     1981 cgcgtggctc gcgtgggtcc tcggcgcgca gtccggcaac gacgcggtgt tcgcgctgct
     2041 cgccggattg gtgatggtcg cgatgggcgc gtggatctac ggacgctcgc ttcatgccga
     2101 gggaatctgg cgcccgaccc ttgccgcgat cctcgtcgcc ggggggctcg ccgtcgcatg
     2161 gccgggcggc gcgatggatg cctccggttc cgccgcggcg gccacggacg atctgcaatg
     2221 gcaggcgtgg agtcccgaga aggtgcgcgc cctcaccgcc gcgggccggc ccgtcttcgt
     2281 cgatttcacc gccgcatggt gcgtcacctg ccaggtgaac aagcgcgtcg cgctgcacaa
     2341 cgcctcggtc atcggcgcat tcgcggcgca cgacgtcgcg accctcaagg ccgattggac
     2401 gcggcaggat ccgcgcatca cggccaccct cgctgcgctg gggcgcaacg ccgtgccggt
     2461 ctacgccctc tatcttccgg gcgaggatgc gccgcgcctt cttccggaag tgctcacgcc
     2521 cgccctcgtc ctcgccgaga tcgaaaagct cccggcggcg aagacggccg tcacgcaacg
     2581 ctgaagacag gagagaagcc atgagaccgc tgaaagggaa gaccatgcga cacgcctcga
     2641 tcgttatcct cgccgccgtg accctcttca tcagtgggtg cgccacgatg tcggcgcaaa
     2701 ctcccggcgc cggcctgagc ccggtgaacg ccatccgcga cggcggcgac gagcacgtga
     2761 tcctgttcgg ccacgacgtg gtgagctatt tcaccgaagg cacgcaccgc atgggtttgc
     2821 cctcgatcaa atcggaatac aagagcgtca ccttccgctt ctcgaagccc gagaacaagg
     2881 cgctcttcga cgcggcgccc gaaaaataca tcccgcagtt cggcgcctat tgcaccaacg
     2941 gcatcgtgta cgccatcccc gccggcggca atcccgatac ctggaagatc atcgacggca
     3001 agctctacat cttcggcggc cagggcgcgc tcgacggctt catgctcgac gtgccgcgca
     3061 acatggcgct ggcccacaag tactgggccg aggaggtgaa cggctcgaac ccgttcgtcc
     3121 agcgttacaa gcgctggatc ttccgcgtgc cccactacgt gaccggcaag gagctcgccg
     3181 agcaggtggc gaaggcgaag aagggctaag gagttttttt tccgcggcgg gcgtgatatc
     3241 ccggccatgc cgggcgaata acgcttcgac atgagcccgc caaccgcacc cgagcccgcc
     3301 cacgagcaat tc
//
LOCUS       DASVII010000021         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_109036,
            whole genome shotgun sequence.
ACCESSION   DASVII010000021 DASVII010000000
VERSION     DASVII010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109036"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VF309_00260"
     CDS             <1..528
                     /locus_tag="VF309_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7465040.1"
                     /translation="NPGKANIGTVNIGSTQNLAGELFKASAGVDAQIVPFNGTPALMS
                     AVLGGSVDVAIEILGPVMPQVAAKSMRVLALMGDKRREGLPGVPTVQESGIANFSVSS
                     WNALAAPARTPPDIVAYLSREAHAAVNSPAVKARLAELGVDARSSTPQEARALLTSEM
                     RRWADVIDKAGIEKQ"
     gene            525..1391
                     /locus_tag="VF309_00265"
     CDS             525..1391
                     /locus_tag="VF309_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7465041.1"
                     /translation="MKTIAPPHPNPTKPAFLLPPGSCDAHCHVFGPAARFPYAPERRY
                     TPPDAPAANLEALHRQLGISRKVLVQASAHGFDNSAMLDAIARDPANCRGVAMVSEDI
                     ADAVLASLHRQGVRALRFNFVRHLGGAPDLGTVSRMAARIAKLGWHLVLHLDAEDLLE
                     YGGFLDTLRIPFVIDHMGRVAAGSGLSQQPFVRLLELMKNELAWVKVSGAERVSSTLG
                     DFADAVPFAAALVDAAPDRVLWGTDWPHPNVREMPDDGRLVDLIALFAKDEIRRRKLL
                     VDNPASLYWGSR"
     gene            1401..>1627
                     /locus_tag="VF309_00270"
     CDS             1401..>1627
                     /locus_tag="VF309_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465042.1"
                     /translation="MSAAQDPKVERLARLDCCAVSDALDKLKLGGVVTGLQRLSTTRR
                     IAGRAVTVKLGTGIAPAGPARHLGTTAIEASR"
BASE COUNT          257 a          595 c          534 g          241 t
ORIGIN      
        1 aaccccggca aggccaacat cggcaccgtc aatatcggca gcacgcagaa cctcgcgggc
       61 gagctcttca aggcgagcgc cggggtcgac gcgcagatcg ttccgttcaa cggcaccccg
      121 gcgctcatgt ccgccgtgct cggcggatcg gtcgacgtgg cgatcgagat cctcggtccg
      181 gtcatgccgc aggtcgccgc gaaatcgatg cgcgtgctcg ccttgatggg cgacaagcgc
      241 cgcgaaggcc tgccgggagt gcccaccgtc caggaaagcg gcatcgcgaa cttcagcgtg
      301 tcctcctgga atgcgctcgc cgcgccggca cgcaccccgc cggatatcgt cgcttacttg
      361 agccgcgaag cgcacgcggc ggtgaattcg ccggcggtca aggcacgcct cgccgagctc
      421 ggagtggacg cgcgttccag cacgccgcag gaggcgcggg cgctcttgac gagcgagatg
      481 cggcggtggg ccgacgtcat cgacaaggcg ggcatcgaaa agcaatgaag accatcgcgc
      541 cgccccatcc caatcccacc aagcccgcat tcctgctgcc acccggatcg tgcgacgcgc
      601 actgccacgt gttcggaccc gccgcgcgat ttccgtacgc acccgagcgc cgctacaccc
      661 cgcccgacgc gcccgccgcg aacctggaag ccctgcaccg ccagctcgga atttcccgca
      721 aggtgctggt gcaggcgagc gcccacgggt tcgacaacag cgcgatgctc gatgcgatcg
      781 cgcgcgatcc cgccaattgc cgcggcgtcg cgatggtctc cgaggacatc gcggacgccg
      841 ttcttgcatc cctgcatcgg caaggcgtgc gcgccctgcg gttcaatttc gtgcgccacc
      901 tgggcggcgc ccccgatctc ggcaccgtga gccgcatggc cgcccggatc gccaagctgg
      961 gctggcacct ggtgctgcac ctggacgccg aggacctgct ggagtacggc ggcttcctcg
     1021 acacgctgcg catcccgttc gtgatcgacc acatgggccg tgtcgcggcg ggaagcggat
     1081 tgtcccagca gcccttcgtg cggctgctcg agctcatgaa gaacgagctc gcatgggtca
     1141 aggtgagtgg cgccgaacgc gtctcctcga cgctcggcga tttcgccgac gccgtgccgt
     1201 tcgccgcggc gcttgtcgac gcggcccccg accgcgtcct gtggggcacc gattggccgc
     1261 atccgaacgt gcgcgagatg cccgacgacg ggcggctggt ggatctcatc gcgcttttcg
     1321 cgaaggacga aatccgccgc cggaagctgc tcgtggataa cccggcaagc ctctactggg
     1381 gttcgcgcta agctaccccc atgagcgccg cccaggatcc gaaagtcgag aggctcgcgc
     1441 gcctcgactg ttgcgcggtc tccgacgccc tcgacaagct caagctcggc ggcgtggtga
     1501 ccggcctgca gcggctatcc accacgcgcc gcatcgcggg ccgcgccgtc accgtcaagc
     1561 tcggtaccgg catcgcgccc gcgggcccgg cgcgccatct cggaaccacg gcgatcgagg
     1621 cgtcccg
//
LOCUS       DASVII010000022         5560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_540467,
            whole genome shotgun sequence.
ACCESSION   DASVII010000022 DASVII010000000
VERSION     DASVII010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540467"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VF309_00275"
     CDS             <1..1250
                     /locus_tag="VF309_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7465043.1"
                     /translation="LAPLVAGVLSSAALAEQTVTMWSHWPDEVVKRAFIEERVRDFEA
                     ANKDCKVKLQFIQKADLYTQAKTSVRTGQAPDIFWLEPDEIAFAQNGYLEPLDGFVNM
                     ANLEDFAKRSWGHNGKVYGLPLEAYTVELYYNRDLLKKIGVSMPVSMQLDQAGFEDMV
                     RKSVAAGITPFATGVSDRPYPGAYFLQEMLLRKLGPDDYRALLTGKLSFKDARVLETM
                     TWVKKLVDEGAYSKSFSTLKLGESHYYFHTNPGALMFPIASWYTGRAFVPPDKGGQPK
                     DFPLGIMQYPAPNGATCPECKTLAVGGSYVMYSKGKNKECAGRLLNSMATVENGNKWM
                     QGVLLQTGIKTDPSRIESIHAPYFRELQERNAKANYFIGTPLQYMAGKCADAFSQILN
                     RAFPGGQMSVNDAATQMDAACKA"
     gene            1255..2154
                     /locus_tag="VF309_00280"
     CDS             1255..2154
                     /locus_tag="VF309_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7465044.1"
                     /translation="MKGIDARAASWPALMVFLLPALAIYAAFTAYPVFRTFYNSFLHI
                     ADLDTARYVGLAHFRELFTDNTFRAAVRNTMIWSFVAPVFDVGTGLLLALCLYAGVPF
                     ARFFRVAWFTPVLLSYVVVGIMWLWIYNYDWGVVNAMLRAIGLPGLAHSWLGDPDTAL
                     AALMVTHAWKWAGFNMVVCLAAMHSLPSEVLEAAELDNCGWGGKMFHIVIPLLRPTLL
                     NLYILAFIGKMKVFDLVFIMTDGGPLWHTETVSTYVYKRAFNWNTFDLGYPSAIAVLW
                     FLVVVAFVALLTWLFRQKDRLEF"
     gene            2205..2990
                     /locus_tag="VF309_00285"
     CDS             2205..2990
                     /locus_tag="VF309_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7465045.1"
                     /translation="MLVLYTLYSALPFVWIGTMSVRTTSEISADHYAWPATWHWSKFH
                     AAWVDSNFGTYFWNSTVVVVAAVAVVTLLGAAAAHGLARYRFRGNRLIYFVLFSSIIF
                     PPQIILISLFQVMAGFGLYNTRLGLTLVYIGLQLPLTVYLLEGFFSRIPQDLFDAAKI
                     DGYSDFEIFWRIVLPVGMPAIATTVILNFIQLWNEFLFAVVLVNDPALSTLPIGIRRF
                     MGDYFQDTGMIATGMMIAVLPVIVVYAFFSEKLIQGMTAGAIK"
     gene            3034..4167
                     /gene="ugpC"
                     /locus_tag="VF309_00290"
     CDS             3034..4167
                     /gene="ugpC"
                     /locus_tag="VF309_00290"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7465046.1"
                     /translation="MAVVRLDNLSKRYPGGAEPAVDSVSFTVADGEFMVLLGPSGCGK
                     SSTLRMIAGLEAISDGDLWIDGRQVNHTPAKDRDIAMVFQSYALYPHMTVRDNLAFGL
                     RRRKVPAGEIERRVALAAGILGLEPLLERKPFALSGGQRQRVALGRAIVREPKVFLFD
                     EPLSNLDAALRVATRNELVKLHRRLGVTMIYVTHDQVEAMTMGQRICVMNRGRVAQIG
                     APLDVYRNPADAFVARFLGSPPMNLLAATLHFDGARGTAIAGKHSIALERWRPETLAR
                     HSQKEILCGIRPEALEMWPLSPGSAVAGGDLVGTLSVIEPLGAETLVLVDLGEGAGEI
                     TARVRNEVDAPIGAPVKLSYPSAAVHLFDKDTQRAIPPDRDRS"
     gene            4164..5150
                     /locus_tag="VF309_00295"
     CDS             4164..5150
                     /locus_tag="VF309_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7465047.1"
                     /translation="MMRIGLVGAGFVTRHHLIAWRAQHPRAKVVAIADPIVEKAQGRA
                     REFDIPSVFSSAAQLLDDGAIDALDVASPRETHAEVVLLAAERGIPVLCQKPLAPNLA
                     EARRVVAAIGARIPVMIHENWRFRRYYREAKRWLDAGVAGRVLQARMTVLSSGLIPDA
                     TGKCPALERQPFMRTLDRMLVMEVLIHHLDTLRFLLGTLTVAGSRIGRASPEIRGEDS
                     AMILLATASGAPVVLHGNMAAQGYPPALVDRMDLFGDRATIALEHGRLTATGRQIAEA
                     RFDLDETYQGSYNDAIAHFVDALLSGDAFETGLQDNLGTLEIVEQIYALDGR"
     gene            complement(5151..>5560)
                     /locus_tag="VF309_00300"
     CDS             complement(5151..>5560)
                     /locus_tag="VF309_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7465048.1"
                     /translation="MTVNLVAHSDLFRAGVATSGSYNKTLTPFGFQNERRSVWEALYV
                     YIKVSPFFAADKMKTPLLIVHGMDDANPGTTPLQSSKLYEAIRGNGGTTRLVLLPNEP
                     HWYAAMESNEQLVFEMLRWFDKYVKDAAPRVGR"
BASE COUNT          921 a         1846 c         1754 g         1039 t
ORIGIN      
        1 tcctggcgcc cctcgtggcc ggcgtcctgt cctcggccgc gctcgccgag cagaccgtga
       61 cgatgtggag tcactggccc gacgaggtgg taaagcgcgc gttcatcgag gaacgcgtga
      121 gggacttcga ggccgcgaac aaggactgca aggtgaagct gcagttcatc cagaaggcgg
      181 acctctacac gcaagccaag acctcggtgc gcaccggcca ggcgcccgac atcttctggc
      241 tcgagcccga cgagatcgcc ttcgcgcaga acggctacct cgagccgctc gacggcttcg
      301 tcaacatggc gaacctcgag gacttcgcca agcgctcgtg gggccacaat ggcaaggtct
      361 acggcctgcc gctcgaggcc tataccgtgg agctctacta caaccgggat ctgctcaaga
      421 agatcggcgt gtcgatgccc gtgagcatgc agctcgacca ggcgggtttc gaggacatgg
      481 tcaggaaatc ggtcgcggcc ggcatcacgc ccttcgccac cggcgtgagc gaccggccct
      541 atccgggcgc ctatttcctg caggagatgc tgctgcgaaa gctcgggccc gacgactacc
      601 gcgcattgct caccggaaag ctctcgttca aggatgcgcg ggtcctcgaa accatgacgt
      661 gggtgaagaa gctggtggac gagggtgcct attcgaagag cttctcgacc ttgaagctgg
      721 gggaatcgca ctactacttc cacacgaatc ccggcgcctt gatgttcccc atcgccagct
      781 ggtacacggg gcgcgccttc gtgccgccgg acaagggcgg gcagcccaag gatttcccgc
      841 tcggcatcat gcagtacccg gcgccgaacg gcgccacgtg ccccgagtgc aagacgctcg
      901 ccgtgggcgg cagctacgtg atgtattcga agggcaagaa caaggaatgc gccggaagat
      961 tgctgaattc catggcgacc gtggagaacg gcaacaagtg gatgcaaggc gtgctgctgc
     1021 aaaccggcat caagacggac ccttcgcgca tcgagagcat tcacgcgccg tacttccgcg
     1081 agctgcagga gcgcaacgcc aaggcgaact acttcatcgg cacgccgctg caatacatgg
     1141 ccggcaagtg cgcggacgcc ttctcgcaaa tcctcaaccg cgcgttcccc ggtggccaga
     1201 tgagcgtgaa cgacgccgcc acgcagatgg acgcggcctg caaggcgtga cggcttgaag
     1261 ggaatcgatg cgcgcgcggc gtcgtggccg gcgctgatgg tgttcctgct gcccgcgctc
     1321 gcgatctacg cggcgttcac cgcctatccc gtctttcgca cgttctacaa cagcttcctc
     1381 catatcgccg acctcgacac ggcgcggtat gtcggcctcg cgcactttcg cgagctcttc
     1441 acggacaaca cgtttcgcgc cgcggtgcgc aacacgatga tctggtcctt cgttgctccc
     1501 gtcttcgacg tgggcacggg cctccttctt gccctgtgcc tctatgcggg cgttcccttc
     1561 gcgaggttct tccgcgtggc gtggttcacg ccggtgctgc tctcgtatgt cgtggtcggg
     1621 atcatgtggc tgtggatcta caactacgat tggggcgtgg tgaacgcgat gctgcgcgcg
     1681 attggcctcc ccggcctcgc gcactcgtgg ctcggcgatc ccgacaccgc tctcgccgcc
     1741 ctcatggtga cccacgcgtg gaaatgggcg ggcttcaaca tggtggtgtg ccttgcggca
     1801 atgcattcgc tcccctcgga agtgctcgag gccgcggagc tcgacaactg cggctggggg
     1861 ggcaagatgt tccacatcgt cattcccctg ctgcggccga cgctgctgaa tctctacatc
     1921 ctcgccttca tcggcaagat gaaggtgttc gacctggtgt tcatcatgac tgacggcggc
     1981 ccgctctggc acaccgagac cgtctcgacg tacgtgtaca agcgcgcgtt caactggaac
     2041 acgttcgacc tgggctatcc ctccgccatc gccgtgctct ggttcctcgt cgtggtcgcg
     2101 ttcgtggcgc tgctcacctg gcttttccgc cagaaggacc ggctggaatt ctgatgggag
     2161 agcccgggcc tctgcggctg cttcgaagcg ccccgctggc ggccgtgctg gttctctaca
     2221 ccctgtattc cgcgctgccc ttcgtctgga tcgggacgat gtcggtgcgc acgacctcgg
     2281 aaatcagcgc cgaccattac gcgtggcccg cgacgtggca ttggtcgaag tttcacgccg
     2341 cctgggtgga ttcgaacttc ggcacctact tctggaacag caccgtggtc gtggtcgccg
     2401 cggtggccgt ggtgacgctg ctgggcgcag cggcggccca tggcctcgcg cgctaccgct
     2461 tccgcggcaa ccggctcatc tacttcgtcc tcttcagctc gatcatcttt ccgccgcaga
     2521 tcatcctcat ttcgctcttc caggtgatgg ccggcttcgg cctctacaac acgcggctcg
     2581 gattgacgct ggtctacatc ggcctgcagc tgccgctcac ggtctacctg ctggaaggct
     2641 tcttctcgcg cattccgcag gacctcttcg acgccgcgaa gatcgacggc tattccgatt
     2701 tcgagatctt ctggcgcatt gtgctccccg tcggcatgcc ggcaatcgcc accaccgtaa
     2761 tcctcaactt catccagttg tggaacgagt tcctgttcgc cgtggtgctg gtcaacgatc
     2821 cggcgctcag cacgcttccg atcggcatcc ggcgattcat gggcgactac ttccaggaca
     2881 ccggaatgat cgcgaccggc atgatgatcg ccgtgctgcc ggtgatcgtc gtatatgcct
     2941 tcttctcgga aaagttgatc cagggcatga ccgccggggc catcaaatga attcccgcat
     3001 cttccaccgc cttgtttccg taagtgggca accgtggccg tagtccgcct cgacaacctc
     3061 tcgaagcgct acccaggtgg cgcggagccc gcggtggaca gcgtgtcctt tacggtcgcc
     3121 gatggggagt tcatggtgct cctcgggcct tcgggatgcg gcaagtcgag cacgctgcgg
     3181 atgatcgcgg ggctcgaggc catcagcgac ggcgacctct ggatcgacgg gcgccaagtc
     3241 aaccatacgc cggcaaagga tcgcgacatc gctatggtgt tccagtccta tgcgctgtac
     3301 ccgcacatga ccgtgcgcga taacctcgcg ttcgggctgc gccggcgcaa ggtcccggcc
     3361 ggcgagatcg agcgccgcgt cgcgctcgcc gcgggcatcc tggggctcga gccgctcctg
     3421 gagcgcaagc ccttcgcgct ctccggcggg cagcgccagc gcgtcgcgct cggacgcgcg
     3481 atcgtgcggg agcccaaggt gttcctcttc gacgagccgc tttcgaatct tgatgcagcg
     3541 ctgcgggttg cgactcgcaa cgaactggtg aagctccacc gccgcctcgg agtcaccatg
     3601 atctacgtga cccatgatca ggtggaagcc atgaccatgg gccagcgcat ctgcgtcatg
     3661 aaccggggac gggtggcgca gatcggcgcg ccgctcgacg tctatcgcaa tcccgccgac
     3721 gcgttcgtag cgcgcttcct gggcagccca ccgatgaacc tgttggccgc gaccctgcat
     3781 ttcgacggcg cgagagggac cgcgatcgcc ggaaagcatt cgatcgcgct cgagcgttgg
     3841 cgccccgaga ccctcgcgcg ccattcgcag aaagagatcc tttgcgggat tcggccagag
     3901 gcgctcgaga tgtggccgct ttcccccggg agcgcagtgg cgggaggcga cctcgtgggc
     3961 acgctctccg tcatcgagcc gctcggagcg gaaacactgg tgctcgtcga tctcggcgaa
     4021 ggggccggag aaatcaccgc gcgtgtgcga aacgaggtcg atgcgcccat cggcgcgccg
     4081 gtcaagctgt cgtatccgag cgcggccgtg cacctgttcg acaaggacac gcagcgcgcc
     4141 attcctcccg atcgggaccg ctcatgatga ggatcggcct cgtcggcgcg ggattcgtga
     4201 cgcggcatca cctgatcgcg tggcgagcgc agcacccgcg cgcgaaggtc gttgccatcg
     4261 ccgatccgat tgtcgagaag gcgcagggcc gagcgcgcga gttcgatatt ccgtcggtct
     4321 tttccagcgc ggcgcagctg ctggacgacg gcgcgatcga cgcgctggac gtcgccagcc
     4381 cgcgcgaaac ccacgccgaa gtcgtgctgc tcgccgccga acgcggcatc ccggtgctct
     4441 gccagaagcc gctcgccccg aaccttgccg aagcgcggcg tgtggtcgcc gccataggcg
     4501 ctcgcatccc cgtgatgatc cacgagaatt ggcgtttcag gcgctactac cgcgaggcga
     4561 agcgctggct cgacgccggg gtggcaggac gcgtgcttca ggcgcgcatg accgtgctga
     4621 gctccggcct cattcccgac gccaccggaa agtgccccgc gctcgagcgc cagcccttca
     4681 tgcgcacgct cgaccgcatg ctggtgatgg aggtccttat ccatcatctc gacacgctgc
     4741 gattcctgct gggcacgctc actgtggcgg gctcgcgcat cggccgggct tcgcccgaga
     4801 ttcgtggcga ggacagcgcc atgatcctgc tggccacggc gagcggcgcg cccgtcgtcc
     4861 tgcacggcaa catggccgcg caaggttatc caccggcgct ggtcgaccgc atggacctct
     4921 ttggcgatcg cgcgacgatc gctctcgagc acggccggct cacagcgacg ggcaggcaga
     4981 ttgcggaagc gcgattcgac ctcgacgaga cctaccaagg atcgtacaac gatgcgatcg
     5041 cgcatttcgt cgatgcgctt ctctccggcg acgcgttcga gaccggtttg caggacaact
     5101 tgggaacgct cgagatcgtc gaacagatct atgcgctgga tgggcgctga ctacctcccc
     5161 acccggggcg ccgcatcctt cacatacttg tcgaaccacc gcagcatctc gaataccagc
     5221 tgctcgttgg actccatcgc cgcgtaccag tgcggctcat tgggcaacag caccagtcgc
     5281 gtcgtgccgc cgttgccgcg gatcgcctcg tagagcttgc tcgattgcag cggcgtagta
     5341 ccggggttgg cgtcgtccat tccgtgcacg atgagtagcg gcgtcttcat cttgtcggcc
     5401 gcgaagaagg gcgagacctt gatgtagacg tacagcgctt cccacaccga gcggcgctcg
     5461 ttctggaagc cgaagggcgt gagcgtcttg ttgtacgacc cgctcgtggc cactcccgcg
     5521 cggaagaggt ccgaatgcgc gaccaggttt accgtcatga
//
LOCUS       DASVII010000023         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_612352,
            whole genome shotgun sequence.
ACCESSION   DASVII010000023 DASVII010000000
VERSION     DASVII010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612352"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="VF309_00305"
     CDS             <1..1631
                     /locus_tag="VF309_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7465049.1"
                     /translation="VVLLAGLVLLPLFWLTVTSLTDDAKHFTLAHYRHFLDDESFVKP
                     LVTTLWTSAAVGVLCVLAAAPMGWLVARTRLPFKRLFRTLILASFVTPPFLGGFAWVL
                     LGGPNAGLVNQWYYALFDLKPFEAQPLINVFTAWGMVFVMALYTFPYVFTFVANSLDV
                     IPSDLEEASSILGAPAWRTALHVTLPLVLPALLAGFLVAFLQSMTLFGVPAILALPAG
                     IDTMTTKIWSLFQFPPQLGLAAAASLPLLVVTVVLLRAQAAIMGRRGYSVIGGKSTGQ
                     RLIKLGPWTVPALALFVFVLGCSIILPYGVLVRTAFVKNWSGPVKGNFTLEHWRFVFF
                     EFSQTKDALLNTFILGISAATAGTLLATTVGYLSIRKLVWGHRYLAFLATAPVAIPGI
                     VLAVGLFLSYTHPPLLLYGTLSIIFLAYLTKELPVGYQQIAASLKSVHPELEDAGRIF
                     GATRLRTLFDVTAPLIRNGVIATWIFIFIGSIRELSATILLFTARTKTISVTMFDLRE
                     SNDWGPIAVLSITMLVITFMMVAAITHFTNRRLT"
     gene            1628..>2581
                     /locus_tag="VF309_00310"
     CDS             1628..>2581
                     /locus_tag="VF309_00310"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7465050.1"
                     /translation="MTVPVLGMMLGDYTGIGPEQCARVLADKRLADAARLLVVGDSRV
                     LGQGARDAGVTLEWRAYATPEEVDWSREETPIIDLQNLDPSIGFPRGVMSPQSGRITG
                     ETLAFMIGLALEGRLDGIVFAPLNKGALHAGGWKFNDEHQMFANLTKHQGFFGEMNVL
                     DNKWMSRVTSHVSLRTALDQITPQRIDDALTLADATMRGAGIAKPRIAVAALNPHGGE
                     NGLFGREEIEIIAPAVAKGAARGIACKGPFPADTVFIKGFGDEYDGVLTMYHDQGQIA
                     TKLKGFNRGVTVTGGLATVFATPAHGTAFDIVGKGIATTGAL"
BASE COUNT          381 a          944 c          805 g          451 t
ORIGIN      
        1 tggtggtgct gctggcgggg ctggtgctgc tgccgctctt ctggctcacg gtcaccagcc
       61 tcaccgacga cgcgaagcat ttcacgctcg cccattaccg gcacttcctc gacgacgaga
      121 gcttcgtgaa gccgctcgtc acgacgctat ggacctcggc cgcggtgggc gtgctgtgcg
      181 tgctcgcggc ggcgcccatg ggatggctcg tggcgcgcac gcgcctgccg ttcaagcgcc
      241 tcttccgcac gctgatcctc gcctcgttcg tgaccccgcc cttcctcggc ggcttcgcgt
      301 gggtgctcct cggcgggccc aacgcggggc tcgtgaacca gtggtactac gcgctcttcg
      361 acctgaagcc cttcgaagcg cagccgctca tcaacgtctt caccgcctgg ggcatggtgt
      421 tcgtgatggc gctctatacg ttcccctacg tcttcacctt cgtcgccaac agcctcgacg
      481 tgatcccgag cgacctcgag gaagcctcct cgatcctcgg cgcgcccgcg tggcgcacgg
      541 cgctccacgt gacgctcccc ctcgtgctcc ccgcgctcct cgcgggcttc ctcgtggcgt
      601 tcctccagtc gatgacgctc ttcggcgtgc ccgcgatcct cgcgctcccc gcgggaatcg
      661 acacgatgac caccaagatc tggagccttt tccagtttcc gccgcagctg ggcctcgccg
      721 ccgcggcctc gctgccgctc ctcgttgtca ccgtcgtgct gctgcgcgcg caggcggcga
      781 tcatgggccg gcgcggctac tcggtgatcg gcggcaagag caccggccag cgcctcatca
      841 agctgggccc ctggaccgtg cccgcgctcg cgctcttcgt cttcgtgctc ggctgctcga
      901 tcatccttcc ctacggcgtg ctggtgcgca cggcgttcgt gaagaactgg tcgggccccg
      961 tgaaaggcaa cttcacgctc gagcactggc gcttcgtgtt cttcgaattc agccagacca
     1021 aggacgcgct cctcaacacg ttcatcctcg gcatatccgc ggcgaccgcg ggcacgctcc
     1081 tcgcgacgac cgtcgggtac ctgagcattc gcaagctcgt gtggggccac cgctacctcg
     1141 ccttcctcgc gaccgcgccc gtggcgatcc cggggatcgt gctcgcggtg gggctttttc
     1201 tcagctacac gcacccgccg cttcttctat acggaacgct ctccatcatc ttcctcgcgt
     1261 acctcaccaa ggagctcccg gtgggctacc agcagatcgc ggcgtcgctg aagagcgtgc
     1321 accccgagct cgaggacgcg gggcgcatct tcggggcgac gcggctgcgc acgctcttcg
     1381 acgtgaccgc gcccctcatc aggaacggcg tgatcgccac ctggatcttc atcttcatcg
     1441 ggtcgatccg ggagctctcg gcgacgatcc tgctgttcac cgcgcgcacc aagacgatct
     1501 cggtcacgat gttcgacctg cgcgagagca atgactgggg gcccatcgcc gtgctatcca
     1561 tcaccatgct cgtgatcacc ttcatgatgg tggcggcgat cacgcacttc accaaccgga
     1621 gactgacatg accgttcccg tcctcgggat gatgctcggc gactacaccg gcatcggccc
     1681 cgagcaatgc gcgcgcgtcc tcgcggacaa gcgcctcgcc gatgccgcgc ggctgctggt
     1741 ggtgggcgac tcgcgcgtgc tcggccaggg cgcgcgcgac gcgggcgtga ccctcgagtg
     1801 gcgcgcctac gccacgcccg aggaggtcga ctggagccgc gaggagacgc cgatcatcga
     1861 cctgcagaac ctcgacccgt cgatcggctt cccgcggggc gtgatgtcgc cgcaatcggg
     1921 gcgcatcacc ggagagacgc tcgccttcat gatcggcctc gccctcgaag ggcgcctcga
     1981 cggcatcgtc ttcgcgccgc tcaacaaggg ggcgctgcac gccggcggct ggaaattcaa
     2041 cgacgagcac cagatgttcg cgaacctcac gaagcaccag ggcttcttcg gcgagatgaa
     2101 cgtgctcgac aacaagtgga tgtcgcgcgt gacctcgcac gtatcgctgc ggacggcgct
     2161 cgaccagatc acgccgcagc gcatcgacga cgcgctcacc ctcgccgacg ccaccatgcg
     2221 cggcgcgggc atcgccaagc cgcgcatcgc ggtggcggcg ttgaaccccc acggcggcga
     2281 gaacggtctc ttcggccgcg aggagatcga gatcatcgcg cccgccgtcg cgaagggggc
     2341 ggcgcgcggc atcgcgtgca aggggccctt ccccgccgac accgtcttca tcaagggctt
     2401 cggcgacgag tacgacggcg tcctcacgat gtaccacgac caggggcaga tcgcgaccaa
     2461 gctgaagggc ttcaatcgcg gcgtcacggt caccggagga ttggccaccg tgttcgcgac
     2521 acccgcccac ggcaccgcgt tcgacatcgt cggcaaggga atcgcgacca ccggtgcgct
     2581 c
//
LOCUS       DASVII010000024         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_360851,
            whole genome shotgun sequence.
ACCESSION   DASVII010000024 DASVII010000000
VERSION     DASVII010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360851"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VF309_00315"
     CDS             <1..383
                     /locus_tag="VF309_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7465051.1"
                     /translation="GGWLWTGDLGAFDADGFLTLKDRSKDLIVSGGSNVYPREVEEVL
                     LAHPAVAEASVVGRPDREWGEVVVAFVVPRGEAPTAGELDQLCLDHIARFKRPREYRF
                     VERLPKNNYGKVVKTELRAALEHD"
     gene            376..1272
                     /locus_tag="VF309_00320"
     CDS             376..1272
                     /locus_tag="VF309_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7465052.1"
                     /translation="MTDLFPGFRRERVRANGIDINAVVGPKRDAPALLLLHGYPQTLA
                     IWHKVATRLAERFNVVATDLRGYGDSSKPPGSPTHDPYSKREMAKDQVEAMAALGHGR
                     FFLCGHDRGARVSHRLAVDHGARVAKVALLDIAPTLAMYEQTTEAFARAYWHWFFLIL
                     PEHLPERMIAADAKLFLKKKMGAGRAGLAPFTPEAWAEYERCFTAGAIHAMCEDYRAS
                     ATIDLVHDRADRDAGRRVTCPLLALWGEDGIIGRCFKPLDEWRRVADDVRGHGVPGGH
                     YIPEEAPDILVAELQKFFLEGA"
     gene            1269..2324
                     /locus_tag="VF309_00325"
     CDS             1269..2324
                     /locus_tag="VF309_00325"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX7465053.1"
                     /translation="MKNYRIAVVAGDGIGKEVVPEGQRVLEAAGRKFGFSLAWDEKPW
                     SCEYYAKHGKMMPDDGLDQIRGHDSIFLGAVGFPGVPDHVSLWGLLIPIRRGFHQYVN
                     LRPVRLMPGVPSPLANRKPGDIDFWVVRENNEGEYSSVGGRMYGGTPEEFATQLAIFS
                     RKGTDRVMKFAFELARTRKKKHVTSATKSNGIAITMPFWDERFKEMSAKYPDVKTSQY
                     HIDILTAHFVQHPDWFDVVVGSNLFGDILSDLGPACTGTIGIAPSANMNPEREYPSCF
                     EPVHGSAPDIYGKGIANPIGQIWSGAMMMGFLGHRDAEDAIVAAIERVLKEGPRTRDM
                     GGTANTVDVGKAIADAL"
BASE COUNT          427 a          823 c          839 g          390 t
ORIGIN      
        1 gcggcggctg gctgtggacc ggcgacctcg gggcgttcga cgcggacgga ttcctcacgc
       61 tgaaggatcg cagcaaggac ctcatcgtga gcggcggatc gaacgtctat ccgcgcgagg
      121 tcgaggaagt gctcctcgcg caccccgcgg tggccgaggc gagcgtcgtc ggccgccccg
      181 accgcgagtg gggcgaggtg gtcgtggcct tcgtcgtgcc gcgcggagaa gcgccgacag
      241 caggcgagct cgaccagctc tgcctcgacc acatcgcgcg cttcaagcgc ccgagggaat
      301 atcgcttcgt cgagcgcctg ccgaagaaca actatggcaa ggtggtgaag accgagctgc
      361 gagcggccct cgaacatgac tgaccttttt cccggattcc gccgtgagcg cgtgcgcgcc
      421 aacggcatcg acatcaacgc cgtcgtgggc ccgaagcgcg atgcgccggc gctcctgctg
      481 ctgcacggct atccccagac cctcgcgatc tggcacaagg tcgcaacgcg cctggcggag
      541 cgcttcaacg tcgtcgcgac cgacctgcgc ggctacggcg attcctcgaa gccgcccgga
      601 agccccacgc acgatcccta ctcgaagcgc gagatggcga aggaccaggt cgaggcgatg
      661 gcggcgctcg gccacgggcg cttcttcctg tgcggccacg atcgcggcgc gcgcgtctcc
      721 catcggctcg cggtcgacca cggcgcgcgc gtggccaagg tggcgctcct cgacatcgcg
      781 cccacgctcg cgatgtacga gcagaccacc gaagcgttcg cgcgcgccta ctggcactgg
      841 ttcttcctca tccttccgga gcacctgccc gagcggatga tcgcggccga tgcgaagctt
      901 ttcctgaaga agaagatggg cgcgggcagg gcggggctgg cgccgttcac gcccgaggcg
      961 tgggccgagt acgagcgctg cttcaccgcg ggggcgatcc acgcgatgtg cgaggactat
     1021 cgcgcctcgg cgaccatcga cctcgtccac gaccgcgccg atcgcgacgc gggccggcgc
     1081 gtgacgtgcc cgctcctcgc gctgtggggc gaagatggca tcatcgggcg ttgcttcaag
     1141 ccgctcgacg aatggcggcg cgtcgccgat gatgtgcgcg ggcacggcgt gccgggcggg
     1201 cactacattc ccgaagaggc cccggatatc ctcgtggccg agctgcagaa atttttcctg
     1261 gagggcgctt gaagaattac cggatcgcgg tggtcgccgg cgacggcatc ggcaaggagg
     1321 tcgttcccga gggccagcgg gtgctcgagg ccgcggggcg caagttcgga ttctcgctcg
     1381 catgggacga gaagccgtgg agctgcgagt actacgcgaa gcacggcaag atgatgcccg
     1441 acgacggtct ggaccagatc cgcgggcacg actcgatctt cctcggcgcc gtcggctttc
     1501 ccggcgtgcc cgatcacgtg tcgctgtggg ggctcctcat cccgatccgc cgcggcttcc
     1561 accagtacgt gaacctgcgc ccggtgcgcc tgatgcccgg cgtgccctcg ccgctcgcca
     1621 accgcaagcc cggcgacatc gacttctggg tggtgcgcga gaataacgag ggcgaatact
     1681 cgtcggtggg tgggcgcatg tacgggggca cccccgagga gttcgcgacg cagctggcga
     1741 tcttctcgcg caagggcacc gaccgcgtca tgaagttcgc cttcgagctc gcgcgcacga
     1801 ggaagaagaa gcacgtgacc tcggccacca agtccaacgg catcgcgatc accatgccct
     1861 tctgggacga gcgcttcaag gaaatgagcg cgaagtatcc cgacgtgaag acttcccagt
     1921 accacatcga catcctcacg gcgcacttcg tgcagcaccc ggactggttc gacgtggtcg
     1981 tcggctccaa tctcttcggc gacatcctct cggacctcgg ccccgcgtgc accggcacga
     2041 tcggcatcgc gccctcggcg aacatgaatc ccgagcgcga atacccgtcg tgcttcgagc
     2101 cggtccacgg cagcgcgccg gacatctacg gcaagggcat cgccaatccc atcgggcaga
     2161 tctggtccgg cgcgatgatg atgggcttcc tcggccatcg cgacgccgag gacgccatcg
     2221 tcgccgcgat cgagcgcgtg ctgaaggagg gcccgcgcac gcgcgacatg ggtggaacgg
     2281 ccaataccgt ggatgtggga aaggcgattg ccgacgcgct ataaacagcg gctggcggat
     2341 acgaaaagct ttggaaacgc agatgaacgc agatgtagcg cagatgaacg taaagctctc
     2401 gggcgggggc tcgcgccatt ggatgcggga atctcgattc caatacttga tggggtacgt
     2461 ccatctgcgt gcctctatc
//
LOCUS       DASVII010000025         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_325036,
            whole genome shotgun sequence.
ACCESSION   DASVII010000025 DASVII010000000
VERSION     DASVII010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325036"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VF309_00330"
     CDS             complement(<1..1145)
                     /locus_tag="VF309_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465054.1"
                     /translation="MAILGFLPIANWLPGGEVDNAYAHRFVEWGYGTAICLGVAVIAA
                     LLWGRKDRATDGLIAAPRSADGSSRFELLERLVPAGALALYVIIARAVFSGRPLLIDE
                     VVQLFQARIFTAGHLWLPVAAHREFFSILHVVDTGDKVYSQFPPGGPAMLAIGELVRA
                     PWLVGPVCGAISVALFSRLVRLTDPGATPAFHLGATLLFAIAPFGAFMFGSHMNHATT
                     LLWLLVATLGLAKVAGSETPRLGWAFANGLGLGAAAAIRPLDAFAFALPAAAWLLVLA
                     WRDKRRWADAIASGIGVVIPFGAMMWVNAQSTGSPLVFGYEVLWGKAHGLGFHTAPWG
                     AMHTPSRGLELISLYVTRLQTYLFETPFPAMLPVIAALALTRKLSAL"
     gene            complement(1157..>1535)
                     /locus_tag="VF309_00335"
     CDS             complement(1157..>1535)
                     /locus_tag="VF309_00335"
                     /inference="COORDINATES: protein motif:HMM:NF025300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465055.1"
                     /translation="GTAPLVHPVRVADLAKRQGASLSALFAATGVNEELSRDALISAE
                     LELKYAGYFVKERNAADRMKRMGEYALPHDAPYEQMRTLSVESRQKLAARRPLSLAQA
                     ASVPGVSPADLQNLILEIERSRS"
BASE COUNT          306 a          500 c          502 g          227 t
ORIGIN      
        1 tcgagcgcgg acaatttcct ggtgagcgca agcgcggcga tgaccggcag catcgccggg
       61 aacggcgtct cgaagagata ggtttgcaac cgcgttacgt acagcgagat cagttctagg
      121 ccgcgcgagg gcgtgtgcat cgcgccccac ggcgcggtgt ggaaacccag accgtgcgct
      181 tttccccaca acacctcata tccgaacacg agcggcgacc ccgtgctctg cgcgttcacc
      241 cacatcatcg cgccgaacgg aatcacaacg ccgatgcctg atgcgatcgc atccgcccac
      301 cgccgcttgt cgcgccaggc gagcaccaat agccaggccg cggcgggcag cgcgaacgca
      361 aaagcatcga gcggccggat cgccgcggcg gctccgagcc cgaggccgtt tgcgaacgcc
      421 catcccagtc gcggcgtttc acttcccgcg accttcgcga ggccgagcgt cgcgacgagc
      481 agccagagca gcgtcgtcgc gtggttcatg tgcgacccga acatgaacgc gccgaacggc
      541 gcgatggcga acagcagcgt cgcgccgaga tgaaatgcgg gcgtcgcgcc gggatccgtg
      601 agcctgacga gtcgcgaaaa aagtgcgacg gaaatggcac cgcacacagg acccacgagc
      661 cacggcgcgc gcacgagctc gccgatcgcg agcatcgcgg gaccgccggg cgggaattgc
      721 gaatagacct tgtcgccggt gtcgacgacg tgaaggatcg agaagaactc ccgatgtgcc
      781 gcgaccggaa gccagagatg gcccgcggta aaaattcggg cctggaagag ctgcacgact
      841 tcgtcgatca gcagcggacg tccggagaaa acggcgcgag cgatgattac gtagagcgcg
      901 agcgcgcccg cgggaaccag ccgttcgaga agttcgaacc gacttgaacc gtcggcgctg
      961 cgtggcgcgg caatcaaccc gtcagtcgcg cgatcctttc ggccccacag cagcgccgca
     1021 attacggcga cacccaaaca aatcgccgtt ccgtatcccc actcgacaaa tcgatgtgcg
     1081 tatgcgttgt cgacctcgcc accgggaagc caattggcga tcgggaggaa tccgaggatc
     1141 gccaatgcga tcgcgatcag gagcgcgatc gttcgatctc cagtatgagg ttctgcagat
     1201 ccgcgggact cacgccgggc acgctcgccg cctgtgcgag cgaaagaggt ctgcgcgccg
     1261 cgagtttctg gcgcgactcc acggaaagcg tgcgcatctg ctcgtacggc gcgtcgtggg
     1321 gaagggcata ctcgcccatg cgcttcatcc ggtcggcggc gttgcgttct ttcacgaaat
     1381 agccggcgta cttgagttcg agctccgcgc tgatcagggc atcgcgcgag agttcttcat
     1441 tgacgccggt cgccgcgaac aacgcgctga gcgaggcgcc ctgccgcttc gcgagatccg
     1501 cgacgcgaac gggatgcacg agtggtgcgg tgccc
//
LOCUS       DASVII010000026         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_433038,
            whole genome shotgun sequence.
ACCESSION   DASVII010000026 DASVII010000000
VERSION     DASVII010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433038"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1813)
                     /gene="rpsA"
                     /locus_tag="VF309_00340"
     CDS             complement(155..1813)
                     /gene="rpsA"
                     /locus_tag="VF309_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7465056.1"
                     /translation="MSDLLAQSSFDKLKEGAIVPGTITEIRQNEVVVDIGGKSEGLVP
                     ANEFLDIGELQIGSTIEVLVQKLEDKNGAPVLSFDLAEQKKNWDNILTKFPEGSVAAG
                     RVKAKVKGGLIISIGVDAFMPASHIDVQPPKNLDQYVGQTYDFKVLKINLDRKNIVLS
                     RRELIEEQRTSKRRNLLESIQPGQIRKGVVKNITDFGAFIDLDGMDGLLHITDMSWGR
                     ISHPSEMLKQGEEVQVMIIEVNREKERVSLGLKQTTKNPWDEIDRKFPVGAKVHGKVV
                     NLVPYGAFVEIEPGVEGLVHITEMSWTKRITKPSELLKVGQELDAVVLGIQKDEQKIS
                     LGLRQLEPNPWDMVRHNYPIGARVRGKVRNMTTYGAFIELEEGIDGMVHVSDMSWTRK
                     VNHPSEVLKKGDEVDAIVLDVDSNQQRISLGMKQLAVDPWSDIDAFFKIGDVVQGTVS
                     KITSFGAFVELKDGIDGLVHISQISEERIEKIKDVLKPGQQVSARVIKIDREERRLGL
                     SIKAANYSAEQLAAETTAYDALNRQSGTDMMNLGDILDEASKKE"
BASE COUNT          359 a          622 c          702 g          473 t
ORIGIN      
        1 acgcggatca cgggcagcga tggcgagcgg gcgggcacgg ctgggttgtg gggattttgg
       61 cgcgcttggc aacaggcggg cgtaaaaaag ccgcccctcc cgctaagcgg gatcggggcg
      121 gcttgaattt ttcgcgaagg aattcgcgcg aggcttattc cttcttggac gcctcgtcga
      181 ggatgtcgcc gaggttcatc atgtcggtgc cactctggcg gttgagcgcg tcgtaagccg
      241 tggtttcggc tgcgagctgc tcagcgctgt agttggcggc cttgatgctc aggccgaggc
      301 ggcgctcttc gcggtcgatc ttgatgacgc gcgcgctgac ctgctggccg ggcttgagca
      361 cgtccttgat cttctcgatg cgctcctcgc tgatctgcga gatgtgcacg aggccgtcga
      421 tgccgtcctt cagctccacg aaggcgccga acgacgtgat cttcgaaacc gtgccctgca
      481 cgacgtcgcc gatcttgaag aaggcgtcga tgtccgacca cgggtcgacc gcgagctgct
      541 tcataccaag gctgatgcgc tgttggttcg aatcgacgtc gagcacgatc gcgtccactt
      601 cgtcgccctt cttgaggact tcggagggat ggttgacctt gcgggtccat gacatgtcgg
      661 agacgtgcac catgccgtcg atgccctctt cgagttcgat gaaggcgccg taggtggtca
      721 tgttgcggac cttgccgcgc acgcgtgcac cgatcgggta attgtggcgg accatgtccc
      781 acgggttggg ctcgagctgg cggaggccga gcgagatctt ctgctcgtcc ttctggatgc
      841 cgaggaccac cgcatcgagc tcctggccga ccttgagcag ctcggagggc ttggtgatgc
      901 gcttcgtcca ggacatctcg gtgatgtgca cgaggccctc gacgccgggt tcgatttcca
      961 cgaacgcgcc gtagggcacg aggttgacga ccttgccgtg aaccttggcg ccgaccggga
     1021 acttccggtc gatttcgtcc cagggattct tggtggtctg cttgaggccg agggaaacgc
     1081 gctctttctc gcggttcacc tcgatgatca tgacctgaac ctcttcaccc tgcttgagca
     1141 tttcggaggg gtgcgagatg cggccccaac tcatgtcggt gatgtggaga aggccgtcca
     1201 tgccgtcgag gtcgataaac gcgccgaagt cggtgatgtt cttgaccacg cccttgcgga
     1261 tctggccggg ctggatgctc tcgagcaggt tgcggcgctt gctggtgcgc tgctcctcga
     1321 tgagctcgcg acgcgagaga acgatgttct tccggtcgag gttgatcttg aggaccttga
     1381 aatcgtaggt ctggccgacg tactgatcga ggttcttggg cggctgcacg tcgatgtgcg
     1441 acgcgggcat gaacgcatcg acgccgatgc tgatgatgag gccacccttg accttggcct
     1501 tcacgcggcc ggcggccacg gaaccctcgg ggaacttggt gaggatgttg tcccagtttt
     1561 tcttttgctc agcgaggtcg aaggaaagga cgggcgcgcc gttcttgtct tcgagcttct
     1621 ggacgagcac ctcgatggtg gagccgattt gcagctcgcc gatgtcgaga aattcgttgg
     1681 cggggacgag accttcggac ttgccgccga tatcgacgac aacttcgttc tggcggattt
     1741 cggtgatggt gcccgggaca atcgcgcctt ccttgagttt atcaaaggac gactgggcga
     1801 gcaggtctga cattactgaa ctcatatgcg gaaaaccggc gaacagacgc tttgctccgg
     1861 aagcgccagc ccaccggtga tgaccccgcc gtaaagcggg gccgtgggtt gactgttgaa
     1921 ctaaggtgaa aagccggcgg gagcatcgcg gcgacgccag cttgaccgat aaactatgcc
     1981 gcgaacggtc agattccctc gcccccaccc ctgccgcaag ccgaaattta gtcctttttc
     2041 ggggcggtta gcgcctgcgc tatcccgttg aagaacacct cgtaatacgg cgtgtccttc
     2101 aacttcgtct ccgtcgcgaa tcccggccgg ttctgggagt cctcctcgac ggcttc
//
LOCUS       DASVII010000027         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_145410,
            whole genome shotgun sequence.
ACCESSION   DASVII010000027 DASVII010000000
VERSION     DASVII010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145410"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /gene="gltA"
                     /locus_tag="VF309_00345"
     CDS             complement(<1..1302)
                     /gene="gltA"
                     /locus_tag="VF309_00345"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7465057.1"
                     /translation="MEAKGKVTLNYGPDKNLDLPTYGGSIGPDVFDIRALYGKTGMFT
                     YDPGFMSTASCQSKITYIDGDKGVLLYRGYPIEQLAVNCDFLEVCYLLLNGELPKKEE
                     KDKFDWTVTRHTMVHEQLARLFQGFRRDSHPMAVMVGVVGALSAFYHDSLDINNPEHR
                     EISAFRLIAKLPTIVAMAYKYNMGQPFMYPRNDLAYVPNFMRMMFGVPAEEYQQNDVL
                     NRALDRIFILHADHEQNASTSTVRLAGSSGANPFACIASGIACLWGPAHGGANEAALQ
                     MLIDLEKEGGIAKVGEFVKKVKDKNSGVKLMGFGHRVYKNYDPRAKLMQETTREVLAA
                     VGAENDPLLPLAMALEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAL
                     ARTVGWIAQWNEMISDPEQKIGRPRQLYIGPTQRDVVPIARR"
     gene            complement(1316..>1522)
                     /locus_tag="VF309_00350"
     CDS             complement(1316..>1522)
                     /locus_tag="VF309_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX7465058.1"
                     /translation="RGLLELDIVFDRFWKGAGTTMDEAEAAALERLLALPDNDLLDLV
                     MGRVEMPEARLRPVLDKLRAAEAP"
BASE COUNT          246 a          484 c          492 g          300 t
ORIGIN      
        1 ccttcttgcg atcggcacca cgtccctttg ggtggggccg atgtagagct ggcggggacg
       61 cccgatcttc tgctcggggt ccgagatcat ttcattccat tgcgcgatcc agcccacggt
      121 gcgggccagc gcgaaaatgc aggtgaacat cgaggtcggg atgccgagcg cgcgctgcac
      181 gatgccggag tagaagtcca cgttggggta cagcttgcgg ctcacgaagt actcgtcctc
      241 gagcgcgatc ttctccagcg ccatcgcgag cggcagcagc ggatcgttct cggcgccgac
      301 cgcggccagc acctcgcgcg tcgtctcctg catgagcttg gcgcgcgggt cgtagttctt
      361 gtagacgcgg tggccgaagc ccatcagctt cacgcccgag ttcttgtcct tcaccttctt
      421 cacgaactcg cccaccttcg cgatgccgcc ttccttctcc aggtcgatca gcatctgcag
      481 cgccgcctcg ttcgcgccgc cgtgcgccgg cccccacagg cacgcgatgc ccgaggcgat
      541 gcatgcgaac ggattcgcgc ccgaggagcc cgccaggcgc accgtggacg tggaggcgtt
      601 ctgctcgtgg tccgcgtgca ggatgaagat gcggtcgagc gcgcggttca gcacgtcgtt
      661 ctgctggtac tcctcggcag gcacgccgaa catcatgcgc atgaagttgg gcacgtaggc
      721 gaggtcgttc ctcgggtaca tgaacggctg gcccatgttg tacttgtagg ccatggcgac
      781 gatggtgggc agcttcgcga tgaggcggaa cgccgagatc tcgcggtgct cggggttatt
      841 gatgtcgagc gagtcgtggt agaaagctga gagcgcgccc accacgccca ccatcaccgc
      901 catcgggtgc gagtcgcggc ggaagccctg gaagaggcgc gcgagctgct cgtgcaccat
      961 cgtgtggcgc gtgacggtcc agtcgaactt gtccttttcc tccttcttcg gcaattcgcc
     1021 attcagcagg agatagcaga cctcgaggaa gtcgcaattc accgcgagct gctcgatcgg
     1081 gtagccgcga tacaggagca cgcccttgtc gccgtcgatg taggtgatct tcgactggca
     1141 cgaggccgtg gacatgaagc ccgggtcgta ggtgaacatc ccggtcttgc cgtagagcgc
     1201 gcgaatgtcg aacacgtccg ggccgatcga tccgccgtag gtcggcaggt cgagattctt
     1261 gtcgggaccg tagttgagtg tgaccttgcc tttggcttcc atcgttcggt ggctcctatg
     1321 gcgcctcggc ggcgcgcagt ttgtccagca caggccggag acgtgcctcc ggcatctcga
     1381 cccgacccat caccaggtcg agcagatcgt tgtcggggag cgcgagcagt ctttcgagcg
     1441 ccgccgcctc ggcttcatcc attgtggttc cggcaccttt ccagaacctg tcgaagacga
     1501 tgtccagctc cagcagcccc cg
//
LOCUS       DASVII010000028         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_145488,
            whole genome shotgun sequence.
ACCESSION   DASVII010000028 DASVII010000000
VERSION     DASVII010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145488"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1426)
                     /locus_tag="VF309_00355"
     CDS             complement(29..1426)
                     /locus_tag="VF309_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7465059.1"
                     /translation="MRALAAIAAVALAGCAIGPDYSRPMLDLPAGYPSGDGSAVAPPA
                     VEDMWWTLYGDATLDDLVAAALAKNPDVEFAVARIEEANAQLREANAAFLPEIDASLN
                     SSRSRVSAATAFPNPPPLVRNDVRGQLSTGFEIDLWGRLRRGDEAARALALGTRFSRE
                     VVFLTLEGLVAQTYFTVRSLDTQIDLSQRTLAARRDSLDVVGARVRGGLASDLDSNAA
                     SIAVADASLQVKELVRQRELAEHQLAVLTSRLDLKLEAAPAAKMPSALPRLPSAGLPS
                     TLLERRPDVRQAEQSLVAANAQVGVAKAAYFPTVSLLAGNGGESTALATLVNAPARIW
                     SVGLNAAMPLLDWGRTTARVDQAAARKDQAVATYRKVAEAAFREVADALTEVRQASIS
                     QREFEDRAATAIDTLRIVDARYRSGLSTYLEVLDAQRTANDAQLSVTRNRLALLNASV
                     SLMKALGGGWRPDAS"
     gene            complement(1423..>2944)
                     /locus_tag="VF309_00360"
     CDS             complement(1423..>2944)
                     /locus_tag="VF309_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7465060.1"
                     /translation="VGLIAFGIIVVAVAGFFRFLPSSFVPIEDQGVLYGSIILPDGAT
                     LTRTGNVAARVQKLLAEHPAVERVFTVTGFDLIGGGNKTNAGTLFITLKPWEDRDVTA
                     DQLTKYIAAKGAEIREGIVISFNPPPIRGLGQAGGLEFYLENRADGDAKHLARVMNDF
                     AAALRKRAELTGINALFRPTVPQVTVEVDNEKAMSLGVPIGDVFDAMQSTMGALYVND
                     FNKFGRTYRVQVQAEAAYRATVESLGNVYVRTAAGEMIPLKALVRTRSVVGPDQIDRF
                     NGFVSAKMLASGVPGVSSGQSIAVVEEEAAKLPDGFTVEWIGQAYQEKRTGKQSIIAF
                     GMALVMVFLILAANYERWSLPVAVLLAVPFAMLGALALVLARGFSNDIYFQIGLVTLI
                     GLAAKNAILIVEFANQEMHAGLSPFDAAVKAARMRFRPIVMTSLAFCLGVVPLVLASG
                     AGAAARRSMGTGVLGGMLVATFVATLFIPLFFVLLAGKGAKPAQAPAPDEPAGEHA"
BASE COUNT          462 a         1018 c         1037 g          427 t
ORIGIN      
        1 gcggctttac ggattcacga gctgagcgtt acgacgcgtc gggccgccag ccgccgccga
       61 gggccttcat cagcgaaacg ctcgcgttga ggagcgccag gcggttgcgc gtgaccgaga
      121 gctgagcgtc gttcgcggtg cgctgggcgt cgagcacttc gagatacgtc gacaatcccg
      181 accgatagcg cgcgtcgacg attcgcagcg tatcgatcgc ggtcgccgcg cggtcttcga
      241 attcgcgttg cgagatgctc gcctggcgca cctccgtcag cgcatcggcg acctcgcgaa
      301 acgcggcttc cgcgaccttg cggtacgtcg ccaccgcctg gtccttgcgg gccgcggcct
      361 ggtccactcg cgccgtggtg cgtccccaat cgagaagtgg catcgcggcg ttcaggccca
      421 cggaccagat gcgcgcgggc gcgttcacga gggtcgcgag cgcggtgctc tcgccgccgt
      481 tgcccgccag cagcgagacg gtcggaaagt acgcggcctt cgcgacgccg acctgcgcat
      541 tcgccgcgac gagggactgc tcggcctggc gaacgtcggg cctgcgctcg aggagggtcg
      601 agggaagccc cgccgacggc agccgcggaa gcgccgacgg catcttcgcc gcgggcgcgg
      661 cctcgagctt gaggtcgagc cggctcgtga gcaccgcgag ctggtgctcc gcgagctcgc
      721 gctggcgcac gagctccttc acctgcagcg acgcgtcggc gaccgcgatc gacgccgcgt
      781 tcgaatcgag gtccgaggcg aggccgccgc gcacgcgggc gcccaccacg tcgagggaat
      841 cccggcgcgc ggcgagggtg cgctgcgaca ggtcgatctg cgtgtcgagc gagcgaaccg
      901 tgaagtacgt ctgcgcgacg agcccctcca gcgtgaggaa cacgacctcg cgcgaaaagc
      961 gcgttcccag cgcgagcgcc cgcgccgcct cgtcgccgcg cctcagtctc ccccagagat
     1021 cgatctcgaa gccggtggag agctggccgc gcacgtcgtt gcgcaccagc ggcggggggt
     1081 tcgggaacgc cgtggccgcg ctcacgcggc tgcgcgacga gttcaggctc gcgtcgatct
     1141 cgggaaggaa cgcggcattc gcttcgcgaa gctgggcgtt cgcctcctcg atgcgcgcca
     1201 ccgcgaactc cacgtcggga ttcttcgcga gcgctgccgc gaccaggtcg tcgagggtcg
     1261 cgtcgccgta gagtgtccac cacatgtcct cgacggccgg cggcgcgacg gcgctcccgt
     1321 cgcccgaggg atagcccgcc ggaaggtcga gcatcgggcg cgaatagtcg gggccgatgg
     1381 cgcaaccggc gagcgctacc gccgcgatcg ccgccaacgc cctcatgcgt gctccccggc
     1441 cggctcatcg ggcgcgggtg cctgcgcggg cttggcgccc ttgcccgcga ggagcacgaa
     1501 gaagagcggg atgaagagcg tcgcgacgaa ggtcgccacc agcatgccgc cgagcacccc
     1561 cgtccccatc gagcgccgtg ccgccgcgcc cgcgcccgag gcgaggacca gcggcaccac
     1621 gccgaggcag aaggcgagcg aggtcatcac gatcgggcga aagcgcatgc gcgccgcctt
     1681 caccgcggcg tcgaagggcg agagccccgc gtgcatctcc tggttcgcga actccacgat
     1741 gaggatcgcg ttctttgccg cgagccctat gagggtcacc aggccgatct ggaaatagat
     1801 atcgttggag aagccccgcg cgagcacgag cgcgagcgcc cccagcatcg cgaacggcac
     1861 ggcgaggagc accgcgaccg gcagcgacca gcgctcgtag ttggctgcga ggatgaggaa
     1921 caccatcacg agcgccatcc cgaaggcgat gatcgactgc ttgcccgtgc gcttctcctg
     1981 gtacgcctgg ccgatccact cgacggtgaa gccatcggga agcttcgcgg cctcctcctc
     2041 gacaacggcg atcgactggc ccgagctcac ccccggcacg ccgctcgcga gcatcttggc
     2101 cgagacgaag ccgttgaacc ggtcgatctg gtccgggccg acgacgctgc gcgtgcgaac
     2161 gagcgccttc agcgggatca tctccccggc ggcggtgcga acgtagacgt taccgagact
     2221 ctcgaccgtc gcgcgatacg ctgcctccgc ctgcacctgc acgcggtacg tgcggccgaa
     2281 cttgttgaag tcgttcacgt agagcgcgcc catggtgctc tgcatcgcgt cgaacacgtc
     2341 gccgatgggc accccgaggg acatcgcctt ctcgttgtcc acttccacgg tcacctgcgg
     2401 aacggtggga cggaagagcg cgttgatccc cgtgagctcg gcgcgcttgc gcagcgccgc
     2461 agcgaagtcg ttcatcacgc gcgcaaggtg cttggcgtcg ccatcggcgc gattctcgag
     2521 gtagaactcg agcccgcccg cctggccgag gccgcggatg ggcggcgggt tgaaggagat
     2581 cacgatgccc tcgcggatct cggcgccctt ggccgcgatg tatttcgtga gttggtcggc
     2641 ggtgacgtcg cggtcctccc agggcttcaa ggtgatgaag agcgtgcccg cgttggtctt
     2701 gttgccgccg ccgatcaggt cgaagcccgt caccgtgaac acgcgctcga ccgcgggatg
     2761 ctcggcgagg agcttctgca cgcgggcggc gacgttgccg gtgcgcgtga gcgtcgcgcc
     2821 gtcgggcagg atgatcgagc cgtagaggac gccctggtcc tcgatcggca cgaagctcga
     2881 cgggagaaag cggaagaatc cggcgacggc cacgacgatg attccgaacg cgatgagccc
     2941 gacg
//
LOCUS       DASVII010000029         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_217396,
            whole genome shotgun sequence.
ACCESSION   DASVII010000029 DASVII010000000
VERSION     DASVII010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217396"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1119
                     /locus_tag="VF309_00365"
     CDS             148..1119
                     /locus_tag="VF309_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465061.1"
                     /translation="MAALLDVRGLATSFTGEEGAFNAVDGVSFTIEAGGTLGLVGESG
                     CGKSVTALSIMGLVASPGKIAAGEILFEGADLLRLAPRAMRDIRGNRIAMIFQEPMTS
                     LNPAFTIGDQIVEGILRHRGIGREAAKRQAIEMLEHVRMPLPAKRFDDHPHRLSGGMR
                     QRAMIAMALACRPKLLIADEPTTALDVTIQAQILELMRMLRDETGAAIILITHDLGVI
                     AELAHDVAVMYAGRIVEKASVAQLFAQPEHPYTIGLLGSIPKLHLRQQRLPAIEGQVP
                     SPAAMPPGCRFAPRCPFVATRCREEDPALRGIHAGQEAACLYAPLAA"
     gene            1116..>1706
                     /locus_tag="VF309_00370"
     CDS             1116..>1706
                     /locus_tag="VF309_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7465062.1"
                     /translation="MTQLLRAENLVKHFPVHGGWRRTGVVHAVDDVSFTLDKGETLAL
                     VGESGCGKSTTGRLVLRLLEATSGKVWFEGNDLFALDESAMRHLRASLQIVFQDPYGS
                     LNPRMTIGQMLDEPLALHGKPVQRSGKQAHRAERIAEILSLVGLRPDHARRYPHEFSG
                     GQRQRIGIARALAAEPKLIVGDEPVSALDVSIQAQVV"
BASE COUNT          265 a          608 c          574 g          259 t
ORIGIN      
        1 ggctgcacgc ggcgagcctc gagcgcttcc agccgttcat gagctgcctg ccaagccgcg
       61 cggagcccgc cggcatcacc gaccgcgcag tgatcgagga acgcatcgcg cggtttcacg
      121 cagcccgcgc caaggcctga ctcttccatg gccgcgcttc tcgacgtccg cggcctcgcg
      181 acctcgttca ccggcgaaga aggcgcgttc aacgcggtgg acggcgtgag cttcacgatc
      241 gaagccggcg gcacgctggg cctggtcggc gagtccggct gcggcaagag cgtgaccgcg
      301 ctttccatca tggggctcgt cgcgagcccg ggaaagatcg ccgccggcga gatcctcttc
      361 gaaggcgccg acctgctgcg ccttgcgccc cgcgcgatgc gcgacattcg cggcaaccgc
      421 atcgcgatga tcttccagga gccgatgact tccctgaatc ccgcgttcac catcggcgac
      481 cagatcgtcg agggaatcct gcgccaccgc gggatcggcc gcgaggcggc gaagcgccag
      541 gcgatcgaga tgctcgagca cgtgcgcatg ccgcttccgg cgaagcgctt cgacgatcat
      601 ccccatcggc tttcgggagg catgcgccag cgcgcgatga tcgccatggc gctcgcgtgc
      661 aggccgaagc tgctcatcgc cgacgagccg accaccgcgc tcgacgtcac catccaggct
      721 cagatcctcg agctcatgcg catgcttcgc gacgagacgg gtgccgcgat catcctcatc
      781 acccacgatc tcggcgtgat cgcggagctc gcgcacgatg tcgccgtgat gtacgccggg
      841 cgtatcgtcg agaaggcgag cgtggcgcaa ttgttcgccc agcccgagca cccgtacacg
      901 atcgggctcc tgggctccat tccgaagctg cacctgcggc agcagcggct gccggcgatc
      961 gagggacagg tgccctcgcc cgccgcgatg ccgccgggct gccgcttcgc gccgcgctgt
     1021 cccttcgtgg ccacgcgctg ccgcgaggaa gatccggcgc tgcgcggcat ccacgcgggc
     1081 caggaggcgg cgtgcctgta cgcgccgctc gccgcatgac gcaactgctg cgcgccgaga
     1141 acctggtcaa gcacttcccg gtgcacggcg gatggcgtcg cacgggggtg gtgcacgcgg
     1201 tcgacgacgt gagcttcacc ctcgacaaag gagagacctt ggcgctcgtg ggcgagtccg
     1261 gctgcggcaa gtcgaccacc ggccggctcg tgctgcggct cctcgaggcc acgtcgggca
     1321 aggtgtggtt cgaaggaaac gatctcttcg cgctggatga atccgcgatg cgccatctgc
     1381 gggcgtcgct gcagatcgtc ttccaggatc cctacggctc gctcaatccg cgcatgacca
     1441 tcgggcagat gctcgacgag cccctcgccc tgcacggaaa gcccgtgcaa cggtccggta
     1501 aacaggcgca tcgggcagag cgcatcgccg agatcctctc gctcgtgggc cttcggcccg
     1561 accacgcgcg gcgctatccc cacgagttct cgggagggca acgccagcgc atcggcatcg
     1621 cgcgcgcgct ggcggccgag ccgaagctca tcgtgggcga cgagccggtc tccgcgctcg
     1681 acgtgtcgat ccaggcgcag gtggtg
//
LOCUS       DASVII010000030         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_397316,
            whole genome shotgun sequence.
ACCESSION   DASVII010000030 DASVII010000000
VERSION     DASVII010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397316"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1749
                     /gene="secA"
                     /locus_tag="VF309_00375"
     CDS             <1..>1749
                     /gene="secA"
                     /locus_tag="VF309_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7465063.1"
                     /translation="QGHEHAEEIMTKAGLLPEGASLYDPTNIMLMHHLYSGIRAHALY
                     HRDQHYVVQNDEVVIVDEFTGRMMQGRRWSEGLHQAVEAKEGTSIKNESQTLASITFQ
                     NYFRLYKKLAGMTGTADTEAFEFSTIYSLETVLIPTHRPMVRADTNDQVFITAKEKYQ
                     AIINDVKEKYANGQPVLLGTTSIENSELISSMLERESLPHQVLNAKQHAREAEIVIQA
                     GRPKMVTIATNMAGRGTDIVLGGNPEPEIQKVKDDPELGDGERQRRIAEMREAWQRLH
                     DEVVNAGGLHIIGSERHESRRIDNQLRGRSGRQGDPGSSRFFLSFEDPLLKIFAGERL
                     QAIMRSLKIPEGEAIEARMVTRSIENAQRKVEARNFDIRKQLLEFDDVANDQRKVIYQ
                     QRNELLESRDISETIASIRRDVLVATVQEFVPPGSVEEQWDLPGLEKKLESEFYIAAP
                     VAKWAEADQALDEDKIAERIVKQADEGYKAKFAPIEDSDTIRNYERAVMLQSIDTHWR
                     EHLAALDYLRQGIHLRGYAQKQPKQEYKREAFELFGAMLDSIKREVTRHLMAVQIRSQ
                     EQVEEAERRAEEAARA"
BASE COUNT          367 a          576 c          574 g          232 t
ORIGIN      
        1 agcagggcca cgagcatgcc gaggagatca tgacgaaggc ggggctgctg ccggaaggcg
       61 cgagcctcta cgatccgacc aacatcatgc tgatgcacca tctctactcg ggcatccgcg
      121 cgcacgcgct ctaccatcgc gaccagcact acgtggtgca gaacgacgag gtggtcatcg
      181 tcgacgagtt caccggccgc atgatgcagg gccggcgctg gtccgagggc ctgcaccagg
      241 cggtggaggc gaaggagggc acctcgatca agaacgagag ccagacgctc gcctcgatca
      301 cgttccagaa ctatttccgc ctctacaaga agctcgccgg catgacgggc acggcggaca
      361 ccgaggcctt cgagttctcg accatctaca gcctcgagac ggtgctgatc ccgacgcacc
      421 gcccgatggt gcgcgccgac accaacgacc aggtcttcat caccgccaag gaaaagtacc
      481 aggcgatcat caacgacgtg aaggagaagt acgcgaacgg ccagccggtg ctcctcggca
      541 cgacgtcgat cgagaattcc gagctcattt cttcaatgct cgagcgcgag agcttgccgc
      601 accaggtgct gaacgcgaag cagcacgcgc gcgaggcgga gatcgtgatc caggcgggcc
      661 gcccgaagat ggtcaccatc gcgaccaaca tggcgggccg gggaacggac atcgtgctgg
      721 gcggaaaccc cgagcccgag atccagaagg tgaaggacga tcccgagctc ggcgacggcg
      781 agaggcagcg gcgcatcgcc gagatgcgcg aggcgtggca gaggctgcac gacgaggtgg
      841 tgaacgccgg cggcctgcac atcatcggct ccgagcgcca cgagtcgcgg cgcatcgaca
      901 accagctgag gggccgctcg ggccggcagg gcgacccggg ctcctcgcgc ttcttcctgt
      961 ccttcgagga tccgctcctc aagatcttcg ccggcgagcg cctgcaggcg atcatgcgct
     1021 ccctcaagat tcccgagggc gaggcgatcg aggcgcgcat ggtgacgcgc tccatcgaga
     1081 acgcgcagcg caaggtcgag gcgcgcaact tcgacatccg caagcagctg ctggaattcg
     1141 acgacgtcgc caacgaccag cgcaaggtca tctaccagca gaggaacgag ctcctcgaat
     1201 cgagggacat ttccgagacc atcgcctcga tccgccgcga cgtgctcgtc gccaccgtgc
     1261 aggagttcgt cccgcccggc agcgtcgagg agcagtggga cctccccggc ctcgagaaga
     1321 agctcgagag cgagttctac atcgcggcgc cggtggcgaa gtgggccgag gccgaccagg
     1381 cgctcgacga ggacaagatc gccgagcgca tcgtcaagca ggccgacgag ggctacaagg
     1441 cgaagttcgc gccgatcgag gattccgaca ccatccgcaa ttacgagcgc gcggtgatgc
     1501 tgcagtccat cgacacccat tggcgcgagc acctcgccgc cctcgactac ctgcggcagg
     1561 gaatccacct gcgcggctat gcgcagaagc agccgaagca ggaatacaag cgcgaagcct
     1621 tcgagctctt cggcgcgatg ctcgactcga tcaagcgcga agtcacgcgc catctcatgg
     1681 cggtgcagat ccgctcccag gagcaggtcg aggaagccga gcgccgcgcc gaggaagccg
     1741 cgcgcgcgg
//
LOCUS       DASVII010000031         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_469241,
            whole genome shotgun sequence.
ACCESSION   DASVII010000031 DASVII010000000
VERSION     DASVII010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469241"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..447)
                     /locus_tag="VF309_00380"
     CDS             complement(100..447)
                     /locus_tag="VF309_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7465064.1"
                     /translation="MSPTNLALTLACVAAIAVGQVFFKLAARAASGAAAFPWDLLNPY
                     LFIALAIYGAATLLWVWLLKTLPLNVAYPFVGLAFVIVPVLGALFLDEALDWRHLAGS
                     ALIASGVAIASWR"
     gene            complement(444..>1536)
                     /locus_tag="VF309_00385"
     CDS             complement(444..>1536)
                     /locus_tag="VF309_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465065.1"
                     /translation="NAITFGFPAVATGAGAALFLGVNPLVDGFDPVFFGLIYDQGAAA
                     RDMSHLSLEGDRILRGVAMVELRDTPLAVVAEMFARKAVALLFFTSVEDSVSPVALMR
                     SWRIALLVFAAAAIVCRAKSRVLVSLAALTAYMLAVHIPVLYHPRYSVGSLDIPLTLL
                     AAIGIAECFARPERIAAAAVAATLGVGAGLLVLQPGLGSPRIERTPSQLIWSRDARQL
                     DHLRLENVLLKGPATFALGPGAAMELTVSEAPLFHPWDISMATFEIAITPAAKTTGCQ
                     AMRLRYRKPDEERFAPEHVTRVALNSDGRMRRITVGAAPLAMNHEGVLRIEFECAFPA
                     TLEMGTISIAAPRRAIYYRELYLKETERR"
BASE COUNT          275 a          509 c          553 g          199 t
ORIGIN      
        1 tcggtcccga tgcccgcgag cacgccgagg atgtgctcgc ggacgcggta gcacgggatc
       61 accaccgcga tgcgcggccg cggctcggcg ctcatcgcgt caacgccatg aagcgatggc
      121 cacgcccgag gcgatgaggg cgctcccggc caggtggcgc caatcgagcg cctcgtcgag
      181 gaagagagcc ccgagcaccg gcacgatgac gaacgcgagc ccgacgaagg gataggcgac
      241 gttgagcggc agcgtcttga ggagccacac ccagagcagg gttgccgccc catagatggc
      301 gagcgcgatg aacagatagg gattgagcaa gtcccacggg aaagccgcgg cgccgcttgc
      361 cgcgcgcgcc gcgagcttga agaagacctg gcccaccgcg atcgcggcga cgcaggcgag
      421 tgtcaatgcg aggttcgtcg ggctcatcgc cgctccgttt ccttcaggta aagctcgcgg
      481 tagtagatgg cgcgccgcgg cgctgcgatc gagattgtgc ccatttccag cgtcgcggga
      541 aacgcgcact cgaattcgat ccgcagcacg ccctcgtggt tcatggcgag cggggccgcg
      601 ccgaccgtga tccggcgcat ccggccgtcg gaattcaagg ccactcgggt gacatgctcc
      661 ggggcaaagc gctcctcgtc gggcttgcga tatcgcaggc gcatcgcctg gcatcccgtc
      721 gtcttcgcgg cgggcgtgat ggcgatctcg aaggtcgcca tcgagatatc ccaggggtgg
      781 aagaggggcg cttcgctcac cgtgagctcc atcgcggcgc cgggccccag cgcgaaggtc
      841 gcgggcccct tgagcagcac gttctcgagg cgcaggtggt cgagctggcg ggcatcgcgc
      901 gaccagatga gctgggaggg cgtgcgctcg atccgcggcg agcccaggcc cggctgcagg
      961 acgaggagcc ccgcgccgac gccgagcgtg gcggcaaccg ccgcggccgc gatgcgctcg
     1021 ggcctcgcga agcattcggc aatcccgatc gcggcgagga gcgtcagcgg aatatcgagg
     1081 gatcccacgc tgtagcgggg gtgatagagc accgggatgt gcacggccag catgtaagcc
     1141 gtgagcgcgg cgagcgacac caacacgcgc gactttgcac ggcagacgat cgcggccgcg
     1201 gcgaagacga ggagcgcgat ccgccacgac cgcatcaacg cgacggggct gacggagtct
     1261 tccacggagg tgaagaagag cagcgccacc gccttgcgcg cgaacatttc cgcgacgacc
     1321 gcgagcggcg tgtcgcgcag ctcgaccatc gcgacgccgc gcaggatccg gtcgccctcg
     1381 aggctcaggt ggctcatgtc gcgcgccgcg gcgccctggt cgtaaatcag cccgaagaac
     1441 acggggtcga aaccgtcgac cagcggattg acgccgagaa agagcgccgc tccggcgccc
     1501 gtcgccaccg ccgggaaccc gaatgtgatc gcattg
//
LOCUS       DASVII010000032         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_505326,
            whole genome shotgun sequence.
ACCESSION   DASVII010000032 DASVII010000000
VERSION     DASVII010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505326"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VF309_00390"
     CDS             complement(<1..819)
                     /locus_tag="VF309_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX7465066.1"
                     /translation="MASHLFSPFVLRSVAFHNRVFVSPMCQYSSEDGMPNDWHFVHLG
                     SRAVGGASLVFVEASAVTPAGRITPWDAGIWSAAHAEAWRRIAQFLRDHGAVPGIQIA
                     HAGRKASCNKPWLGGKSLAPGEGAWPTLGPSAIPFGHYATPRAMTVHEIERTVEDFRA
                     AAGHALAAGFDVVEIHGAHGYLLHSFASPLSNRREDHYGGSLENRIRLALEVARAVRE
                     TWPRDKPVFYRISATDWYEGGWDIEQSVVLCKKLKEIGIDLIDCSSGGNIHDQKI"
     gene            883..>1609
                     /locus_tag="VF309_00395"
     CDS             883..>1609
                     /locus_tag="VF309_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465067.1"
                     /translation="MLTIRAGKRALARIQSAGLSAADVEIVPGAAGGPKALGIAGLDR
                     ALFGEWLPAAPGVRHLIGSSIGAWRFAAACRGDGMKALDEFARAYTEQSYPHRPTRRF
                     VSQAARDMLAGLFAGREDEILAHPHYRLHVLAVRGRWPLTRDGRFSTPFGFGMAAMAN
                     AVGRRHLARFLERTVFHDPRDAPRFLQTQRFDAFRTHAVALDRANFGEALLASASIPL
                     VLEGVSGIPGAPSGIHWDGGLIDY"
BASE COUNT          232 a          550 c          560 g          267 t
ORIGIN      
        1 gatcttctgg tcgtggatgt tgcctccgct cgagcagtcg atgaggtcga tgccgatctc
       61 cttcagcttc ttgcacagga ccaccgactg ctcgatgtcc cagccgccct cgtaccagtc
      121 ggtggcggag atgcgataga agacgggctt gtcccggggc cacgtttcgc gcaccgcgcg
      181 cgccacctcg agcgccaggc ggatgcgatt ctccagcgag ccgccatagt gatcttcgcg
      241 gcgattcgac aggggcgagg cgaacgaatg caggaggtag ccgtgggcgc cgtggatctc
      301 gacgacgtcg aatccggcgg cgagcgcgtg acccgcggcc gcgcggaagt cctcgacggt
      361 gcgctcgatc tcgtgcaccg tcatcgcgcg aggggtcgca tagtggccga agggaatcgc
      421 gctgggcccg agcgttggcc atgcgccttc gccgggcgcg agggattttc cgcccagcca
      481 gggcttgttg cacgaggcct tgcgccccgc gtgcgcgatc tggatccccg gaaccgcgcc
      541 gtgatcgcga aggaattgcg caatgcgcct ccacgcttcc gcgtgagccg ccgaccagat
      601 gccggcatcc cacggcgtga tgcggccggc cggcgtgacg gcgctcgctt cgacgaacac
      661 cagcgaggcg ccgcccacgg cgcggcttcc caggtggacg aagtgccaat cgttgggcat
      721 gccgtcctcg ctcgagtact ggcacatcgg cgagacgaag acgcggttgt ggaaggcgac
      781 ggagcgcagg acgaagggcg agaagaggtg ggaggccatc ggggtctttt ttgctggata
      841 gtaagggggc gggccactaa cgactccatg atgccaaatt cggtgcttac cattcgtgcg
      901 ggaaagcgcg cactcgcgcg catccaatcg gcgggccttt cggccgccga tgtcgagatc
      961 gttccgggcg cggcgggtgg ccccaaggcg ctcggcatcg ccgggctcga tcgcgcgctc
     1021 ttcggcgagt ggctcccggc ggcgccgggc gtgcgccacc tcatcggctc ctccatcggc
     1081 gcctggcgct tcgctgccgc atgccgcggc gacggcatga aggcgctcga cgagttcgcg
     1141 cgcgcgtaca cggagcagag ctacccgcat cgccccaccc ggcgcttcgt ctcgcaagcg
     1201 gcgcgcgaca tgctcgcggg acttttcgcg ggccgcgagg acgagatcct cgcccatccg
     1261 cactaccgtt tgcacgtgct cgccgtgcgc gggcgctggc cgctcactcg cgacggacgc
     1321 ttctcgaccc cgttcggctt cggcatggcg gcgatggcga acgcggtcgg caggcgccat
     1381 ctcgcgcgct tcctcgagcg taccgtgttc cacgaccctc gcgacgcgcc acgattcctc
     1441 cagacccagc gcttcgatgc gttccgcacg cacgcggtgg cgctcgatcg cgcgaacttc
     1501 ggcgaggcgc tgctcgcgtc ggcgtccatt ccgctggtgc tcgagggcgt gagcggcatt
     1561 ccgggcgcgc cttcgggaat ccattgggat ggcggcctca tcgactacc
//
LOCUS       DASVII010000033         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_109824,
            whole genome shotgun sequence.
ACCESSION   DASVII010000033 DASVII010000000
VERSION     DASVII010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109824"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VF309_00400"
     CDS             complement(<1..710)
                     /locus_tag="VF309_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7465068.1"
                     /translation="MEQDIVRALNRVSLARIAASVVAAVLLASSPGAPAQDFITVAST
                     TSTEQSGLFKHLLPEARKATGIEIRVVAVGTGQALDMGRRGDADVVFVHDLVAEEKFL
                     AEGWGVKRYPVMYNDFVIVGPKADPAGAKGNDVIAAMKKIAAAGAPFASRADKSGTHA
                     AELRYWKMADIEPARLDPKAAWYRETGSGMGPTLNTASGMNAYALADRGTWLSFKNRG
                     DLVILVEGDTKLFNQYGV"
     gene            complement(674..1420)
                     /locus_tag="VF309_00405"
     CDS             complement(674..1420)
                     /locus_tag="VF309_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465069.1"
                     /translation="MASAPYFPLEARGLGVTLGGHAALDAVDLVLDGRARAVVLGPNG
                     SGKSVLLRVLHGLIAPTAGAVLWAGSPARAPDQAMVFQRPVMLRRSALANVEYALALN
                     GVAVSERETRAWEAIDRVGLAYLAKRPARVLLGGEQQRLALARAWALRPRVIFLDEPT
                     ASLDPAAASEVERVIGEIHAAGTAIVMTTHHLGLARRVADEILFLHEGRLTEHTAVED
                     FFTAPRSAEAARFLKGELPWNKTSSGPSTE"
     gene            complement(1413..>1592)
                     /locus_tag="VF309_00410"
     CDS             complement(1413..>1592)
                     /locus_tag="VF309_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465070.1"
                     /translation="SEVERVIGEIHAAGTAIALETTKGDLPLALALGVILLSMVLALN
                     AAAYAIREWSAKRHG"
BASE COUNT          235 a          556 c          561 g          240 t
ORIGIN      
        1 atcacgccgt actggttgaa gagcttcgtg tcgccctcga cgaggatgac gaggtcgccg
       61 cggttcttga aggaaagcca cgtgccgcgg tcggcgagcg cgtaggcgtt catgcccgag
      121 gcggtgttga gcgtgggtcc catcccggaa cccgtctcgc gataccacgc tgcctttgga
      181 tccaatcgcg ccggctcgat gtccgccatc ttccagtagc gcagctccgc cgcgtgcgtt
      241 ccgctcttgt cggcgcgcga ggcgaacggc gccccggccg ccgcgatctt cttcatcgcc
      301 gcgatgacgt cgttgccctt cgcgccggcg ggatccgcct tcgggcccac gatgacgaag
      361 tcgttgtaca tcaccggata gcgcttcacg ccccagcctt ccgcgaggaa tttctcctcg
      421 gccacgaggt cgtgcacgaa caccacgtcg gcgtcgccgc ggcgtcccat gtcgagcgcc
      481 tggcccgtgc ccacggcgac cacgcggatc tcgatgccgg tcgccttgcg cgcttcgggc
      541 agcaggtgct tgaagagtcc cgactgctcg gtcgaggtgg tcgacgcgac ggtgatgaag
      601 tcctgcgccg gcgcgccggg cgacgaggcg agcagaaccg cggcgacgac cgacgccgcg
      661 atccgggcga ggctcactcg gttgagggcc cggacgatgt cttgttccat ggcaattctc
      721 ctttgaggaa tcgcgcggct tcggcggagc gtggcgctgt gaagaagtcc tcgacggccg
      781 tgtgctccgt gagccggccc tcgtgcagga agaggatctc gtcggcgacg cgccgcgcga
      841 gccccaggtg atgggtggtc atgacgatgg cggttccggc ggcgtggatc tcgccgatca
      901 cgcgctccac ttcggacgcc gcggcgggat cgaggctcgc ggtcggctcg tcgaggaaga
      961 tcacgcgcgg gcgcaacgcc caggcgcgcg cgagcgcgag gcgctgctgc tctccgccca
     1021 acaacacgcg cgcgggccgc ttcgccagat acgcgagccc cacgcgatcg atcgcctccc
     1081 aagcgcgggt ttcgcgctcg gagacggcga ccccgttcag cgcgagcgcg tactcgacgt
     1141 tcgcgagcgc cgagcgacgc agcatcaccg ggcgctggaa caccatcgcc tggtccggcg
     1201 cgcgcgcggg ggagcccgcc caaaggaccg cgccggcggt gggcgcgatc agcccatgaa
     1261 gcacgcgcag gagcacgctc ttgcccgagc cgttgggccc gagcaccacg gcgcgcgcgc
     1321 ggccgtcgag aacgagatcg accgcgtcga gcgcggcgtg gcctcccagg gtgacgccga
     1381 ggccgcgagc ttcgagcgga aagtagggcg cgctagccat ggcgcttcgc gctccactcg
     1441 cggatcgcat aggccgcggc gttcaatgcg agcaccatcg acaggaggat cacgcccagc
     1501 gctagagcga gcggcaggtc gcccttggtc gtctccagcg cgatcgcggt gccggcggcg
     1561 tggatctcgc cgatcacgcg ctccacttcg ga
//
LOCUS       DASVII010000034         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_2009, whole
            genome shotgun sequence.
ACCESSION   DASVII010000034 DASVII010000000
VERSION     DASVII010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF309_00415"
     CDS             complement(<1..251)
                     /locus_tag="VF309_00415"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7465071.1"
                     /translation="MLENARRLLAAGNPKQAYAELSAVQDKMTGMPEFDYLLGVAALD
                     SGKTEDAIIAFERVLAVVPNHAGAQMDLARAYYATGSFDL"
     gene            519..1328
                     /locus_tag="VF309_00420"
     CDS             519..1328
                     /locus_tag="VF309_00420"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7465072.1"
                     /translation="MDDRVGEGASPRAEALLQRSDDGGVATLVLNRPGQYNALSRALL
                     EALHAELDAIALDPAVRVVIITGAGRAFCAGHDLKEMRALESEGEVHALFTMCSAMMQ
                     KLTALPQPVIAAVNGVATAAGCQLVAQCDLAVAADDARFAASGINLGLFCSTPAVPLS
                     RNLSRKRAAEMLFTGEFIDAGTALDWGLVNRVAPRERLMAAARELAGVLMAKPREILA
                     IGKALFYRQLEQGLASAYADASGTIARNFSGDIAREGVDAFLAKRPPRWKP"
     gene            complement(1330..>1536)
                     /locus_tag="VF309_00425"
     CDS             complement(1330..>1536)
                     /locus_tag="VF309_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465073.1"
                     /translation="LVGGDIFHGKLSLNQLFSARPVLGNANYRMPVRGVFLCGSGAHP
                     GGGVTGVPGHNAAREVLRDLRRRS"
BASE COUNT          249 a          530 c          510 g          247 t
ORIGIN      
        1 aggtcgaatg acccggtggc gtagtaggcg cgcgccagat ccatctgcgc gccggcgtgg
       61 ttcggcacga cggcgagcac gcgctcgaaa gcgatgatcg cgtcctcggt cttgccgcta
      121 tcgagggccg ccacgccgag caggtaatcg aactcgggca tccccgtcat cttgtcctgc
      181 accgcggaga gctcggcgta ggcctgcttc gggttgcccg cggcgaggag gcggcgcgcg
      241 ttttcgagca ccgaatccgc cggctgagca cgcaccgcga gcgatgcgaa agcaatgaaa
      301 atggcaacga gagccacccc gcatgaccgt gcgcgctctt tcatctggat ttttccttgt
      361 ttttggcttt atccaccccc cgccactccc aggagagcca gcgcggcaag gcggcacaaa
      421 cccatgttat tcctgttgat tccccgtggc aacgcagcat tttcatgagc cgccggtgcg
      481 ataatcctct tgacaggaat ccatgaagga gtagggcaat ggacgatcgc gtgggcgagg
      541 gcgcgagccc gcgggccgaa gcccttctgc agcgtagcga cgacggcggg gtcgcgaccc
      601 ttgtcctcaa ccgccccggg caatacaacg cgctctcccg ggcccttctc gaagcgctcc
      661 acgccgagct cgatgcgatc gcgctcgacc cggcggtgcg agtggtgatc atcaccggag
      721 cgggccgcgc cttctgcgcg gggcacgacc tcaaggagat gcgcgccctc gaatccgaag
      781 gcgaagtcca cgcgctcttc acgatgtgca gcgcgatgat gcagaagctc accgcgcttc
      841 cgcagcccgt gatcgcggcg gtgaacggcg tcgccacggc cgccggctgc cagctcgtcg
      901 cgcaatgcga tctcgcggtt gcggcggacg acgcgcgttt cgccgcgtcg ggaatcaacc
      961 tcgggctgtt ttgctccacg ccggcggtgc ctctgtcgcg caacctttcg cgcaagcgcg
     1021 ccgcggaaat gctcttcacc ggcgaattca tcgacgccgg cacggccctc gattggggcc
     1081 tggtgaatcg cgtggcgcct cgcgagcgcc tgatggccgc ggcccgcgag ctcgccggcg
     1141 tgctcatggc gaagccgcgc gagatcctcg cgatcggcaa ggcgctcttc tatcgacagc
     1201 tggagcaagg cctcgcgagc gcctatgccg atgcctcggg cacgatcgcg cggaatttct
     1261 cgggggatat cgcgcgcgag ggcgtggacg ccttcctcgc caagcgcccg ccgcgctgga
     1321 agccctgact cacgaccggc gccgcaggtc gcgaagcact tcgcgcgcgg cgttgtggcc
     1381 gggcacgccg gtgaccccgc cacccggatg cgcgcccgat ccgcagagga aaacgccccg
     1441 gaccggcatc cggtaattcg cgttgccgag caccggccgc gcgctgaaga gctggttgag
     1501 cgagagcttg ccgtggaaga tgtcgccccc cacgag
//
LOCUS       DASVII010000035         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_505404,
            whole genome shotgun sequence.
ACCESSION   DASVII010000035 DASVII010000000
VERSION     DASVII010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505404"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..559)
                     /locus_tag="VF309_00430"
     CDS             complement(77..559)
                     /locus_tag="VF309_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7465074.1"
                     /translation="MHPHEALVREFYARFAARDAEGMAACYHPDVAFSDPVFPMLRGA
                     EAADMWRMLLSRAADLEVTLDEASGDADGAVARWTARYTFSRTGRPVVNRVRGMFAFR
                     DGRIVRHYDHFSFWRWASQALGPAGKALGWFGPLKWKVRRDAATALERFRERRRSTAT
                     "
     gene            683..>1689
                     /locus_tag="VF309_00435"
     CDS             683..>1689
                     /locus_tag="VF309_00435"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX7465075.1"
                     /translation="MKPIGEIAKKLGIPDAAISPFGHYKAKIALEYVNSLGSKPDGKL
                     VLVTAISPTPAGEGKTTTTVGLGDALNHIGKRAVICLREPSLGPVFGMKGGAAGGGYA
                     QVVPMEDINLHFTGDFNAIQLANNLLSAMIDNHINHGNELGFDVRRIAWKRVLDMNDR
                     ALRQITVALGGPANGFPREDGFDIVVASEVMAIFCLSMSIKDLRERLGKIVCGYTRDQ
                     KPILAKDLKTHGAMAALLKDALAPNLVQTLEHSPAFIHGGPFANIAHGCNSVLATKTA
                     LKLGDYVVTEAGFGADLGAEKFVDIKCRKAGLKPDCVVVVATIRALKMHGGVAKGDLK
                     K"
BASE COUNT          312 a          577 c          557 g          243 t
ORIGIN      
        1 ctgcccggcg tcgcggcgcg cgatgccgcg ctcgtggcca atggcggcgt ccccatgggc
       61 gacctcacca acgcgctcac gttgcggtag aacgcctccg ctcgcggaag cgttccagcg
      121 ccgtggcggc gtcgcggcgc accttccact tgaggggccc gaaccagccg agggccttgc
      181 ccgccgggcc cagcgcctgg gaggcccacc gccagaagga gaagtggtcg taatggcgca
      241 cgatgcgccc gtcgcgaaag gcgaacatgc cgcgcacgcg attgacgacc ggccgcccgg
      301 tgcggctgaa ggtgtagcgc gccgtccatc gcgcgactgc gccgtccgcg tcgccgctcg
      361 cctcgtcgag cgtcacctcg aggtcggcgg ctcgcgaaag gagcatgcgc cacatgtctg
      421 ccgcctccgc gccgcgcagc atcgggaaca ccggatcgct gaaagcgacg tcgggatggt
      481 agcaagcggc catgccttcg gcgtcccgcg ccgcgaatcg ggcatagaac tcccggacca
      541 gggcttcatg gggatgcatg gaaggaattg acagccgtca aggtggcggg ttttggggag
      601 ttcctatact gccgcaaaac ccgcataacc acccgaagga cttcccacgc tcagcgatat
      661 cgagatcgcc caaaaggcga agatgaaacc cattggcgag atcgccaaga agctcggcat
      721 cccggacgcg gcgatttccc cgttcgggca ctacaaggcg aagatcgcgc tcgaatacgt
      781 gaattccctc gggtcgaagc ccgacgggaa gctcgtgctg gtcaccgcca tcagcccgac
      841 ccccgcgggc gagggcaaga cgaccaccac cgtgggcctg ggcgacgcgc tgaaccacat
      901 cggcaagagg gcggtgatct gcctgcggga gccctcgctc ggtccggtgt tcggcatgaa
      961 gggcggcgcc gcgggcggcg gctatgccca ggtcgtcccg atggaagaca tcaacctgca
     1021 cttcaccggc gacttcaacg cgatccagct cgcgaacaac ctcctctcgg cgatgatcga
     1081 caaccacatc aaccacggca acgagctcgg cttcgacgtg cgccgcatcg cctggaagcg
     1141 cgtgctcgac atgaacgacc gcgcgcttcg ccagatcacg gtggccctcg gcggccccgc
     1201 caacggcttc ccgcgggaag acggcttcga catcgtggtc gcctcggagg tgatggcgat
     1261 cttctgcctg tcgatgagca tcaaggatct ccgggagcgc ctcggcaaga tcgtgtgcgg
     1321 ctacacgcgc gaccagaagc cgatcctcgc caaggacctc aagacccacg gagcgatggc
     1381 ggcgctgctc aaggacgcgc tcgcgcccaa cctcgtgcag acgctcgagc actcgcccgc
     1441 gttcatccat ggcggccctt tcgccaacat cgcgcacggc tgcaactcgg tgctcgcgac
     1501 gaagacggcg ctgaagctcg gcgactacgt ggtcaccgag gcgggcttcg gcgccgacct
     1561 gggcgccgag aaattcgtcg acatcaagtg ccgcaaggcg gggctgaagc ccgattgcgt
     1621 cgtggtggtg gcaaccatcc gcgcgttgaa gatgcacggt ggcgtcgcga agggtgacct
     1681 caagaagga
//
LOCUS       DASVII010000036         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_289858,
            whole genome shotgun sequence.
ACCESSION   DASVII010000036 DASVII010000000
VERSION     DASVII010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289858"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VF309_00440"
     CDS             complement(<1..419)
                     /locus_tag="VF309_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465076.1"
                     /translation="MNLVKLKSIISAMVENPVIHPERISEKPIVFAGRIDLEDFPRLE
                     ESLANRDAKLDYTVTARLDKQRRKVVSCIIEGFVFLTCQTTLEAFRHEVSIDDRLVLV
                     DDESQLPPIEEESDAEDYLVADEPIDVLDLVEDAVLLA"
     gene            432..1016
                     /locus_tag="VF309_00445"
     CDS             432..1016
                     /locus_tag="VF309_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX7465077.1"
                     /translation="MTDISLVLASGSPYRRELLARLAVAFETWSPEIDERALAQESPR
                     DTAVRLAHVKAAAALARWPGAIVIGSDQVAEVDGAPIGKPGTLDNARRQLRQLSGRAV
                     AFHTALCVLNGASGKRHDRLVTTDVEFRALSDTDIERYLAREPALDCAGSAKSEGLGI
                     ALLSRLSGDDPTALVGLPLIALAGILRAEGIEIP"
     gene            1013..1726
                     /locus_tag="VF309_00450"
     CDS             1013..1726
                     /locus_tag="VF309_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7465078.1"
                     /translation="MSATGRLFLLPAWLSEDTPPEDVVPAPVLERIRSLDSFIVENAK
                     SARAYLAACGHRKPMREIAMAELNEHTAAADIAELLDGVLAGRDTGLLSEAGLPAIAD
                     PGALLVAAAHGRGVAVVPCVGPSSILLALMASGLDGQRFRFVGYLPAESAARRERIVE
                     LARHSAKHAETQVFIETPYRNEPLLADLLHSCRGSSRLAVAAELTSPREWIRMKTIAG
                     WRKDAAVIGKRPAIFLLLA"
     gene            complement(1754..>2655)
                     /locus_tag="VF309_00455"
     CDS             complement(1754..>2655)
                     /locus_tag="VF309_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7465079.1"
                     /translation="RDAQTLMKNADTAMFHGKGLGKNTYQYFTAQMNIAVKRRMTLES
                     ALRRAVMQKDFVLHYQPQIDLQTGEIVAVEALVRWKTEDSGTVMPGDFIPLAEETGLI
                     NEIGEWVLREGCRQAKEWQDLGLAGSRRMAINLSARQFSDKGFLDMVERVLAETGLAP
                     RCLELEITESQVMRQTEGMIQLLNKLAEMGVHLAIDDFGTGYSSLSYLKRLPIQKLKI
                     DQSFVRDITIDPNDTAIVVAIINMAKSLDLETIAEGVETAGQLALLRSKGCQVGQGFF
                     FSVPLPAQELYPLLRINHVAVEG"
BASE COUNT          415 a          897 c          871 g          472 t
ORIGIN      
        1 gcgaggagca ccgcgtcttc gaccaggtcc aggacatcga tcggctcgtc cgcgaccagg
       61 taatcctccg cgtcgctttc ctcctcgatc ggcggcaact gcgactcgtc gtcgacgaga
      121 accagccggt catcgatgga aacctcgtgc cggaatgctt ccagggtggt ctggcacgta
      181 agaaaaacga agccctcgat tatacatgac actacctttc gacgctgctt gtccaggcgg
      241 gccgtgacgg tgtaatcgag cttggcgtcg cggttcgcca acgattcctc gagcctcgga
      301 aaatcctcga gatcgatccg gcctgcgaag acgatcggct tctcggagat acgctcgggg
      361 tgaatgacgg gattttcgac catggcagat atgatagact ttagcttaac taaattcaat
      421 aagaatctga tttgactgat atttcattgg tgctggcgtc gggctcgccc tatcggcgcg
      481 agctgctagc gcggctcgcg gtcgcgttcg agacatggtc acccgagatc gacgagcgtg
      541 ccctggcaca ggaaagcccc cgcgataccg ccgttcgcct cgcccacgtc aaggccgcgg
      601 cagcgctcgc gcgctggccc ggcgcgatcg tcatcggctc cgaccaggtt gccgaggtcg
      661 atggagcgcc gatcggaaag ccgggcaccc tcgacaatgc gcgccgccag ttgcgccagc
      721 tcagcggccg cgcggtggcg ttccacacgg cgctgtgcgt gctgaacggc gcgagcggca
      781 agcgccatga tcgcctcgtg accaccgacg tcgagtttcg cgcgctctcc gataccgaca
      841 tcgagcgcta cctcgcccgg gagcccgcgc tcgactgcgc gggaagcgcg aagagcgagg
      901 ggctcggcat cgcgctcctg tcgcggctct cgggagacga ccccacggcg ctcgtgggct
      961 tgccgctcat cgcgctcgcg gggatcctgc gagccgaagg catcgagatt ccttgagcgc
     1021 gacggggcgc ctcttcctgc tgccggcgtg gctttccgaa gacacgcctc ccgaggacgt
     1081 ggtccccgcg ccggtgctcg agcgcattcg atcgctcgat tcgttcatcg tcgagaacgc
     1141 gaaatcggcg cgcgcgtatc tcgcggcgtg cggccaccgc aagccgatgc gcgagatcgc
     1201 gatggccgag ctcaacgagc acaccgcggc cgcggacatc gcggagctgc tcgacggcgt
     1261 tctcgccggc agggacacgg gattgctgtc cgaagcgggg ttgcctgcga tcgcggatcc
     1321 gggagcgctt ctggtcgccg cggcgcatgg gcgcggggtc gcggtcgttc cctgcgtcgg
     1381 gccctcctcg atcctgctcg ccctcatggc gagcggcctc gacgggcagc gcttccgctt
     1441 cgtgggatat cttccggccg aatccgccgc gcggcgcgag cgcatcgtgg agctcgcgcg
     1501 tcattccgcg aagcacgccg agacccaggt cttcatcgag acgccgtacc gcaacgagcc
     1561 gctcctcgcg gatctgctgc actcgtgccg cgggtcgtcg cgcctcgccg tcgccgccga
     1621 gctcacgtca ccgcgcgaat ggatccgcat gaagacgatc gcggggtggc gcaaggacgc
     1681 ggcggtcatc ggcaagcggc cggccatctt cctgctgctc gcctgagaaa gccgcggcgc
     1741 ggggagccgg ggcctaaccc tcgaccgcga cgtgattgat gcgaagcagc ggatagagct
     1801 cctgggccgg aagcggcacg gaaaagaaga agccctgccc cacctggcag cccttcgagc
     1861 gcagcagcgc cagctggccc gcggtctcga ccccttcggc gatggtctcg aggtcgaggc
     1921 tcttcgccat gttgatgatc gcgacgacga tcgccgtgtc gttggggtcg atggtgatgt
     1981 cgcgcacgaa cgactggtcg atcttcagct tctggatcgg caggcgcttc aggtacgaca
     2041 ggctcgagta gccggtgccg aaatcgtcga tcgcgaggtg cacgcccatc tccgcgagct
     2101 tgttcagcag ctggatcatc ccttcggtct ggcgcatcac ctggctctcg gtgatctcga
     2161 gctcgaggca gcgcggcgcg aggcccgtct cggcgaggac cctctccacc atgtcgagga
     2221 agcccttgtc gctgaactgc cgcgccgaga ggttgatcgc catgcgccgg cttcccgcga
     2281 ggcccaagtc ctgccactcc ttcgcctggc ggcagccctc gcgcagcacc cactcgccga
     2341 tctcgttgat gaggccggtc tcctcggcga gcgggatgaa gtcgcccggc atcaccgtgc
     2401 cgctgtcctc ggtcttccag cgcaccagcg cctcgaccgc gacgatctcg ccggtctgga
     2461 ggtcgatctg cggctggtag tgcagcacga aatccttctg catcacggcg cgccgcagcg
     2521 ccgattcgag cgtcatgcgc cgcttcaccg cgatgttcat ctgcgcggtg aagtactggt
     2581 aggtgttctt gcccaggccc ttgccgtgga acatcgccgt gtcggcgttc ttcatgaggg
     2641 tctgcgcgtc gcggc
//
LOCUS       DASVII010000037         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_146018,
            whole genome shotgun sequence.
ACCESSION   DASVII010000037 DASVII010000000
VERSION     DASVII010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146018"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VF309_00460"
     CDS             <1..446
                     /locus_tag="VF309_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX7465080.1"
                     /translation="EMRRRHSLIGSMMIHRGEADGLICGTFGTHDLHLHYIDQVIGKR
                     PGIRNYYAMNLLMLPKRTVFICDTYVNLDPTPEQIVEMTMLAEEEIRRFGLAPKVALV
                     SHSNFGTSDAPSARKMQKALEMIREKMPASMVISTICSGVGSRFT"
     gene            complement(452..1576)
                     /locus_tag="VF309_00465"
                     /pseudo
     CDS             complement(452..1576)
                     /locus_tag="VF309_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
BASE COUNT          263 a          517 c          520 g          282 t
ORIGIN      
        1 tcgagatgcg ccggcgccac tcgctgatcg gctcgatgat gatccaccgc ggcgaggccg
       61 acgggctcat ctgcggcacc ttcggcacgc atgatctgca tttgcactac atcgaccagg
      121 tgatcggcaa gcgcccgggg atccgcaact actacgcgat gaacctgctg atgctgccca
      181 agcgcaccgt gttcatctgc gacacctacg tgaacctcga tcccacgccc gagcagatcg
      241 tcgagatgac catgctcgcc gaggaggaga tccgccgctt cggcctcgcg ccgaaggtgg
      301 cgctggtctc gcactcgaac ttcggcacca gcgacgcgcc ctcggcgcgc aagatgcaga
      361 aggcgctcga gatgatccgc gagaagatgc cggcgagcat ggtcatctcg acgatctgct
      421 cgggcgtggg atcgaggttc acgtaggtgt cgcaaatgaa cacggtgcgg ctgggcagca
      481 tcagcaggtt catcgcgtag tagtcgcgga tccccgggcg cttgccgatc acctggtcga
      541 tgtagtgcag gtgaaggtca tgtgtgccga aggtgccgca gatgaggccg tccgcctcgc
      601 cgcggtggat catcatcgag ccgatcagcg tgtgacgccg gcgcatctcg atctgcgcgt
      661 aggcgagcga cacgccgcgg cgctccgcga ggcgatggta ttcggtgaag tactcgcggt
      721 agcgcgggtc gccctcggga ttgacgacct ggaaatccac gccggccttc aggcgcaggc
      781 cgaagcgctc gatgcgccgc tcgagcaccg cggggcgtcc caccaggatc ggcttcgcga
      841 gcccctcgtc cacgatcact tgcacggcac gcagcacgcg ctcgtcttca ccttccgcga
      901 acacgatgcg gcgcggcgcc tccttcgcac gcgtgaagat gggcttcatg atgagcccgg
      961 agtgatagac gaactcgttc agcttctgga cgtacgcggg gaaatccttg atcggatcgg
     1021 tcgccacgcc gctttccatc gccgccttcg ccacggccgg cgcgatcttc acgatgaggc
     1081 gcggatcgaa cggccgcggg atgaggtatt cggggccgaa gctcagctgc tcgctgccgt
     1141 acgcctgcgc gacgatatcc gacgcctcgg cgagcgcgag gtcggcgatc gcgttcaccg
     1201 cggcgagctt catcggctcg ttgatggtgg tcgcgcccac gtcgagcgcg ccgcggaaga
     1261 tgaacgggaa gcagaggacg ttgttcacct ggttcgggta gtcggagcgg ccggtggcga
     1321 tcacggcgtc gtcgcgcacc gccttgatct gctccggatg gatctcgggc tcgggattcg
     1381 ccagcgcgag gatgagcggc ctggaagcca tcttcttcac catctcgggc ttgaggaccc
     1441 cgcccgcgga cagccccagg aacacgtcgg cgccttcgat cacctcggcc aacttgcgcg
     1501 cgtcggtctt gcgcgcatag cggatcttgt tgtcgtccat ctcttccttg cggcccacgt
     1561 agacgacgcc cttgatgtcc gt
//
LOCUS       DASVII010000038         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_74043, whole
            genome shotgun sequence.
ACCESSION   DASVII010000038 DASVII010000000
VERSION     DASVII010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74043"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VF309_00470"
     CDS             <1..527
                     /locus_tag="VF309_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465081.1"
                     /translation="FAAALAAVGIGLAAHGQELLEGYTCCNLHYDKDWISDANWRQLP
                     SIPAGSKIRITSYGWNNRANAQIDGREMRIGHDYGRNEEKLEQYVAKLVVKKDPRAAI
                     ARYPAKIRDAIKDGTVIPGMTREQVIISVGYPPTHRTPRLDAPVWNLWASRAGRYEVH
                     FSDKGVVDKIVGTP"
     gene            complement(542..1315)
                     /locus_tag="VF309_00475"
     CDS             complement(542..1315)
                     /locus_tag="VF309_00475"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7465082.1"
                     /translation="MHADPALIRSTKGRVTADEWAVRTDLAAAYRAIAMYGWDDLVFT
                     HISARVPGPDHHFLINPYGMMFEEITASSLVKIDLAGNKVLESPFEINPAGFTIHSAV
                     HEAREDAHCVIHLHTMEGVAVSCQKAGLLPLSQQSLFPLSSLAYHAYEGVALNAGEKV
                     RLVADLGDKHTMILRNHGLLTCAETVADAFLYMYILQKACEVQVLAQAGGGELIPVAQ
                     PIVDGIRQAAATVLRQSGGMIAWPGILRKLDRADPSWRG"
     gene            1332..>1580
                     /locus_tag="VF309_00480"
     CDS             1332..>1580
                     /locus_tag="VF309_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7465083.1"
                     /translation="MAISIDRIDHIVITAHDVERTLDFYQRVLGMEPITFAGGRRALA
                     FGRQKINLHQAGREYEPKALKPNPGSMDLCFVTETPLDD"
BASE COUNT          281 a          539 c          517 g          243 t
ORIGIN      
        1 gtttcgccgc ggcgctcgcg gccgtgggca tcgggctcgc cgcccatggg caggagctcc
       61 tcgagggcta cacctgctgc aacctccact acgacaagga ctggatcagc gacgcgaact
      121 ggcgccagct tccgtcgatc cccgcgggat cgaagatcag gatcacgtcg tacgggtgga
      181 acaatcgcgc caacgcgcag atcgacggac gcgagatgcg catcggccac gactacgggc
      241 gcaacgagga gaagctcgag caatacgtcg ccaagctggt ggtgaagaag gatccgcgcg
      301 ccgcgatcgc gcgctatccc gcgaagattc gcgacgcgat caaggacggc acggtgatcc
      361 ccggcatgac gcgcgagcag gtgatcatct cggtcggcta tccgcccacg caccgcacgc
      421 cgcgcctcga cgcgccggtg tggaacctgt gggcctcgcg ggcagggcgc tacgaggtgc
      481 acttcagcga caagggagtg gtcgacaaga tcgtcggcac gccgtagcgc cgcgtaggcg
      541 atcagccgcg ccaggatgga tcggcgcggt cgagcttcct cagtattccg ggccacgcga
      601 tcatgccgcc cgactggcgc agcaccgtcg ccgcggcctg cctgatgccg tcgacgatcg
      661 gctgcgcaac cgggatcagc tcgccgccgc ccgcctgcgc gagcacctgc acctcgcacg
      721 ccttctgcag gatgtacatg tagagaaacg cgtccgccac ggtctcggcg caggtgagga
      781 gcccgtggtt gcgcaggatc atggtgtgct tgtcgcccag gtccgcgacc aggcgcacct
      841 tctcgccggc gttcagcgcc acgccctcgt aggcgtggta cgcgaggctc gacagcggaa
      901 agagcgattg ctgcgagaga ggcagaagcc ccgccttctg gcacgacacc gccacgcctt
      961 ccatcgtgtg caagtggatc acgcagtgcg cgtcctcgcg cgcctcgtgc accgcgctgt
     1021 ggatcgtgaa gcccgcggga ttgatctcga agggcgactc cagcaccttg ttgccggcga
     1081 ggtcgatctt caccagcgaa gacgccgtga tctcctcgaa catcatcccg tacgggttga
     1141 tgaggaaatg gtggtccggc ccgggcacgc gcgccgaaat gtgcgtgaac acgaggtcgt
     1201 cccagccgta catcgcgatc gcgcggtacg cggcggcgag gtcggtgcgc accgcccatt
     1261 cgtcggcggt gacgcggcct tttgtcgaac ggatgagcgc cgggtctgcg tgcatgaaat
     1321 aatgttaccc catggccatt tccatcgacc ggatcgacca catcgtcatc accgcccacg
     1381 acgtcgagcg cacgctcgac ttctaccagc gcgtgctcgg catggagccc atcaccttcg
     1441 cgggtgggcg ccgcgcactc gcgttcgggc gccagaagat caacctgcac caggcgggcc
     1501 gcgagtacga gcccaaggcg ctcaagccca acccgggctc catggacctg tgcttcgtca
     1561 ccgagacacc gctcgacgat
//
LOCUS       DASVII010000039         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_505595,
            whole genome shotgun sequence.
ACCESSION   DASVII010000039 DASVII010000000
VERSION     DASVII010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505595"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VF309_00485"
     CDS             complement(<1..606)
                     /locus_tag="VF309_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HEX7465084.1"
                     /translation="MTDDWKRIADRLDAFLARVERLLPASPGEPDWKAGSAYRWRKNA
                     RGGYLEAVRHPHAIRFSDLKDIDEQKRRLRDNTAQFVAGRPANNVLLTGARGTGKSSL
                     VKAALNEFSSRGLRLIEVDKRDLVDLPDIVDMVGHRKERFVVFCDDLSFEASDAGFKS
                     LKVALDGSIAGSAENVLIYATSNRRHLMPEFMGENLEARHID"
     gene            complement(603..1268)
                     /locus_tag="VF309_00490"
     CDS             complement(603..1268)
                     /locus_tag="VF309_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7465085.1"
                     /translation="MADSRGNAPQLEKYYARRASEYEQVYEKAERQDELEWLRGRIPA
                     LLRNRTVLEVACGTGYWTQFIAKSAKKVHACDINEAVLEIAREKPIPRDRVEFYKADA
                     VTLEGVPGGCDAAFAGFWWSHVKRSGIERFVTGLATRLEPRSRVVILDNTYVEGSSTP
                     VTRRDSEGNTYQKRKVASGEEYEVLKNFPTADELRAAVAPIAREAHLESLNYYWLLVF
                     TLK"
     gene            complement(1289..2518)
                     /gene="argJ"
                     /locus_tag="VF309_00495"
     CDS             complement(1289..2518)
                     /gene="argJ"
                     /locus_tag="VF309_00495"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX7465086.1"
                     /translation="MAVNLEAPRPENLLSISGVRLGVARAGIRKPDRKDLLLVALDEG
                     SRVAAVFTRNRFAAAPVIVSRQHLADRDPIRAIVVNTGVANAGTGAVGIANARATCVA
                     VASLLDLENSQVLPLSTGVIMEHLPVDRIKAGLPDCVKDLAASNWASAAEAIMTTDTV
                     PKAVSRQVVIDGCTITVTGISKGAGMIQPNMATMLGIIATDAAVGADALKYLARQAAA
                     ESFNVITVDGDTSTNDTLVLAATRRAGNPEIVGEGTAEFGKLAVTVCEVAQLLAQAIV
                     RDGEGATKFITINVEEGATREECVLVGKAIAHSPLVKTAFFASDPNLGRILAAIGNAG
                     IADLEPSRVGLELGGVTVVEEGARAAGYTEEEGKRVMKQSEISMTVRLGRGVEAATVW
                     TCDLSYDYVKINADYRS"
BASE COUNT          404 a          964 c          927 g          489 t
ORIGIN      
        1 gtcgatgtgc ctcgcctcga gattctcccc catgaattcc ggcatgagat ggcggcgatt
       61 ggaggtcgcg tagatcagca cgttctcggc ggagcccgcg atcgagccgt cgagcgccac
      121 cttcagcgac ttgaatcccg cgtcggaagc ctcgaacgag agatcgtcgc agaacaccac
      181 gaagcgctcc ttgcggtggc cgaccatgtc gacgatgtcg ggaaggtcca ccaggtcgcg
      241 cttgtccacc tcgatcaggc gcaatccccg ggacgagaac tcgttcaacg ccgccttcac
      301 gagcgagctc ttgcccgtgc cgcgcgcgcc ggtgaggagc acgttgttgg cggggcgccc
      361 ggcgacgaac tgcgcggtgt tgtcgcgaag ccgccgcttc tgctcgtcga tgtccttgag
      421 gtccgagaag cgaatggcat gcggatggcg caccgcctcg aggtagccgc cgcgcgcatt
      481 cttgcgccag cgatacgcgg agccggcctt ccagtcgggc tcgcccggcg acgcgggcag
      541 cagccgctcg acgcgggcga gaaacgcgtc gagccgatcg gcgatgcgct tccagtcgtc
      601 cgtcatttca gcgtgaacac cagcagccag tagtagttga gcgactcgag atgcgcttcg
      661 cgcgcgatcg gtgccaccgc ggcgcgaagc tcgtcggccg tgggaaagtt cttgagcacc
      721 tcgtactcct cgccgctcgc caccttgcgc ttctggtagg tgttgccttc ggaatcgcgg
      781 cgcgtgaccg gggtggagct tccttccacg taggtgttgt cgaggatgac gacgcgcgag
      841 cgcggctcga ggcgcgtcgc gaggccggtg acgaagcgct cgatgccgct cctcttcacg
      901 tgcgaccacc agaagcccgc gaacgccgcg tcgcacccgc ccggcacgcc ctcgagcgtc
      961 accgcgtcgg ccttgtagaa ttcgacgcga tccctgggaa tcggcttttc gcgcgcgatc
     1021 tcgagcaccg cctcgttgat gtcgcacgca tggaccttct tcgcgctctt cgcgatgaac
     1081 tgcgtccagt atcccgtgcc gcacgcgacc tcgagcacgg tgcggttcct gagcagcgcg
     1141 gggatgcggc cgcgaagcca ctccagctcg tcctggcgct cggccttttc gtagacctgc
     1201 tcgtattcgg acgcgcgcct ggcgtagtac ttctcgagct gcggggcgtt cccccgggaa
     1261 tcggccatcg gtgctcccct gccggccatc agcttcggta gtcggcgttg atcttcacgt
     1321 aatcgtagga gagatcgcag gtccacacag tcgcggcttc gacgccgcgc ccgagcctca
     1381 cggtcatgga gatctcgctt tgcttcatga cgcgcttgcc ctcctcctcc gtgtagcccg
     1441 cggcgcgggc gccctcctcc acgacggtca cgccgcccag ctcgaggccc acccgcgagg
     1501 gctcgagatc ggcgatcccc gcattgccga tcgcggcgag gatgcggccg agattcgggt
     1561 cggaggcgaa gaacgcggtc ttcaccagcg gcgagtgcgc gattgccttg ccgacgagca
     1621 cgcattcctc gcgcgtggcg ccctcctcga cgttgatcgt gatgaacttg gtggcgcctt
     1681 ctccatcgcg cacgatcgcc tgcgcgagaa gctgcgcgac ctcgcacacg gtgacggcga
     1741 gcttgccgaa ttcggcggtt ccctcgccca cgatctcggg attgcccgcg cgccgcgtcg
     1801 ccgccagcac cagcgtgtcg ttggtcgagg tgtcgccgtc gacggtgatc acgttgaagg
     1861 attccgccgc cgcctgccgc gcgagatact tcagcgcgtc ggcgccgacg gccgcgtcgg
     1921 tcgcgatgat gccgagcatc gtcgccatgt tgggctggat cattcccgcg cccttggaga
     1981 tgccggtgac ggtgatcgtg cagccgtcga tcaccacctg gcgcgacacc gccttcggca
     2041 ccgtgtcggt ggtcatgatc gcttcggccg ccgacgccca gttgctcgcc gcgagatcct
     2101 tcacgcaatc gggcaatccc gccttgatgc gatcgacggg aaggtgctcc atgatcacgc
     2161 ccgtcgacag gggaaggacc tgcgaattct cgagatcgag gaggctcgcc acggccacgc
     2221 acgtcgcgcg cgcgttcgcg atgcccacgg cgcccgttcc cgcgttggcc acgcccgtgt
     2281 tcacgacgat cgcgcggatc ggatcccggt cggcgagatg ctggcgggag acgatgacgg
     2341 gcgcggcggc gaaacggttg cgcgtgaaga ccgccgcgac gcggctgccc tcgtcgaggg
     2401 ccacgaggag cagatccttg cgatcgggct tgcgaatgcc cgcccgcgcg acgccgagcc
     2461 tcacgcccga gatgctgagc aggttttcag ggcgcggcgc ctcgagattg acggccattc
     2521 ttgttgtgtc cggagaaacc gggattttac acggggggtg tcttgcgctt cacccgcgcc
     2581 atcggggggc tgtagacgtg gcgccccgcc gcgcccgcct caagcgagct ttccgtggca
     2641 ctgcttgaat ttcttccctg acccgcaggg gcacggatcg ttgcggccca ccttctgcga
     2701 cgagcgcacg aagggctgcg cgtccgcggt cccgccggcg ccggcgccga gcgcgacggg
     2761 cccgccgctc gccgggttcg ccgc
//
LOCUS       DASVII010000040         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_146123,
            whole genome shotgun sequence.
ACCESSION   DASVII010000040 DASVII010000000
VERSION     DASVII010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146123"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VF309_00500"
     CDS             <1..976
                     /locus_tag="VF309_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7465087.1"
                     /translation="AMGLAAAPALALGAEEPSPASARIVADEFWADKGGVRLWVYRKR
                     IEGARPAKRLFCVHGSSYSGKTMYDLQVPGRDGYSMMDHFARRGYEVWTMDHEGYGHS
                     QKTAGNSDIQSGVDDLKAAMAVVAKEGGPAKLAFFGQSSGVLRAARFANQHPQHVEKL
                     AIDAFVWTGKDAPTLAQRAQNLERYKASNRRPVNREFYESIFTRDHSGAAEPMLGAVI
                     ADEELRYGDSVPTGTYVDMVTKLPLVDPTKLACPVMIIRAEHDGIATDEDILAFFARI
                     PSADKQLLKIAGLAHTALLGVNRGRFFHALDSFLSMPALVDLHGTRQH"
     gene            987..1697
                     /locus_tag="VF309_00505"
     CDS             987..1697
                     /locus_tag="VF309_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7465088.1"
                     /translation="MKDDLRNAKYVIDIGPVASLPVAGSDSRFPVGRIYCVGRNYADH
                     AREMGHDPNREPPFFFMKAANSIVQNHSEIAYPAATKDVHHEIEMVVAIGKGGKNIPV
                     EKALEHVWGYGVGLDMTRRDIQGEAKKLGRPWEMGKSFDESAPVTALRPASEIGHPAK
                     GAIWVKVNGTVKQQGDLSQQIWSVPEQIAYLSNLITLQPGDLIFSGTPAGVGPIVAGD
                     KLEGHVDGVGDLTITYKR"
     gene            1694..1876
                     /locus_tag="VF309_00510"
     CDS             1694..1876
                     /locus_tag="VF309_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465089.1"
                     /translation="MTSFFVSTFAFFIGGFFIRRYLDEIGIERTFGRATIVFFLALAI
                     AYGVAFIVDAVASLFG"
     gene            complement(1885..>2335)
                     /locus_tag="VF309_00515"
     CDS             complement(1885..>2335)
                     /locus_tag="VF309_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465090.1"
                     /translation="GDLQVLAYSKVWKWLVRAFWIFPVVIALVGVFSPPVPGERWIPF
                     AIIGGFTALCWPLTLEVFRRRIELGESGISQQSAWSRPVTIAWKDVRDVAFKLSGDIE
                     LVSTRGKKVRVSVYLSGMETFAETLERRLAHLPSTAAVVKKIRATRV"
BASE COUNT          422 a          794 c          761 g          358 t
ORIGIN      
        1 cgccatggga ctcgccgcgg cgccggcgct cgcgctcggc gccgaagagc cgtcgcccgc
       61 gtccgcgcgc atcgtcgccg acgagttctg ggccgacaag ggcggggtgc ggctgtgggt
      121 ctatcgcaag cgcatcgagg gcgcgcgccc cgcgaagcgc ctgttctgcg tgcacgggtc
      181 ttcctattcg ggcaagacca tgtacgacct ccaggtgccg gggcgcgacg gctattcgat
      241 gatggatcac ttcgcgcgcc gcggctacga ggtctggacc atggaccacg agggctacgg
      301 ccactcgcag aagaccgcgg gcaattccga catccagagc ggcgtcgacg acctcaaggc
      361 ggccatggcg gtcgtcgcga aggagggcgg ccccgcgaag ctcgcgttct tcggccaatc
      421 gtcgggggtc ctgcgcgcgg cgcggttcgc gaaccagcat ccgcagcacg tcgagaagct
      481 cgccatcgac gccttcgtgt ggaccggcaa ggacgcgccc acgctcgccc agcgggcgca
      541 gaacctcgag cgctacaagg cgtcgaatcg acgcccggtg aatcgcgagt tctacgagag
      601 catcttcacg cgcgaccact cgggcgccgc cgagccgatg ctcggcgcgg tgatcgccga
      661 cgaggagctg cgctacggcg actcggtccc gaccggcacc tacgtcgaca tggtcacgaa
      721 gcttccgctc gtcgatccca cgaagctcgc gtgcccggtg atgatcatcc gcgccgagca
      781 cgacggcatc gcgaccgacg aggacatcct cgccttcttc gccaggattc ccagcgccga
      841 caagcagctt ctcaagatcg ccgggctcgc gcacaccgcg ctcctgggcg tgaatcgcgg
      901 gcgcttcttc catgcgctcg actcgtttct ctcgatgccg gccctcgtgg acctgcacgg
      961 cacacggcaa cactgaaagg aaagccatga aagacgacct tcgcaacgcc aagtacgtga
     1021 tcgatatcgg gcccgtcgct tcgctccccg tcgcgggaag cgactcgcgc ttccccgtgg
     1081 ggcgcatcta ctgcgtcggg cgcaactacg ccgaccacgc gcgcgagatg gggcacgacc
     1141 ccaatcgcga gccgccgttc ttcttcatga aggccgccaa ttcgatcgtg cagaaccatt
     1201 ccgagatcgc ctacccggcg gccaccaagg acgtgcacca cgagatcgag atggtggtcg
     1261 cgatcggcaa gggcggaaag aacattcccg tcgagaaggc gctcgagcac gtgtggggct
     1321 acggagtcgg cctcgacatg acgcgccgcg acatccaggg cgaggcgaag aagctgggcc
     1381 gcccgtggga gatgggcaag agcttcgacg agtccgcgcc ggtgacggcg ctgcgccccg
     1441 cgagcgagat cggccatccc gccaagggcg cgatctgggt gaaggtgaac ggcaccgtga
     1501 agcagcaggg cgatctctcg cagcagatct ggagcgtgcc cgagcagatc gcgtatctct
     1561 ccaacctcat cacgctgcag cccggcgacc tcatcttctc cggcacgccg gcgggcgtgg
     1621 ggccgatcgt cgcgggcgac aagctcgaag gccacgtcga cggagtgggg gatctcacca
     1681 tcacctacaa gcgttgacca gcttcttcgt ctcgacgttc gccttcttca tcggcggctt
     1741 cttcatccgg cgctacctcg atgaaatcgg catcgagcgc accttcgggc gcgcgaccat
     1801 cgtgttcttc ctcgcgctgg ccatcgccta cggggtggcg ttcatcgtcg atgccgtggc
     1861 gtcgctcttc ggataggaac cgcctcacac ccgggtagcg cgaatcttct tcaccacggc
     1921 ggcggtcgag ggcaagtgcg cgaggcgcct ttccagggtc tcggcgaacg tctccatccc
     1981 cgatagatag acggagacgc gaaccttctt gccgcgagtc gaaacgagct cgatgtcgcc
     2041 ggacagcttg aaggcgacat cgcgaacgtc cttccaggca atggtcaccg gccgcgacca
     2101 tgcggactgc tgcgagatgc cggattcgcc gagctcgatc ctgcggcgaa agacctccag
     2161 cgtgagcggc cagcagagcg cggtgaagcc cccgatgatg gcgaaaggaa tccagcgctc
     2221 gccgggcacg ggcggagaga agactccgac cagcgcgatg accacgggga atatccagaa
     2281 ggcacggacc agccacttcc agaccttgct gtaagcgagc acctggagat cgccg
//
LOCUS       DASVII010000041         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_38354, whole
            genome shotgun sequence.
ACCESSION   DASVII010000041 DASVII010000000
VERSION     DASVII010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38354"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VF309_00520"
     CDS             complement(<1..472)
                     /locus_tag="VF309_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX7465091.1"
                     /translation="MDHQAKARPDATYLVAPETGRTMSFGSLRSASLRLAAFLRGRHV
                     EPGDKVGLLMHNGYQACRLFIGAMYGGYCVTPFNLLAQPSHLEYVLDHSDVALIFASP
                     DQEPRLREALQKIERAIQVVTCDVDAEEFLPASPDDGEPLAPIGEEDVALMMYTS"
     gene            complement(501..1904)
                     /locus_tag="VF309_00525"
     CDS             complement(501..1904)
                     /locus_tag="VF309_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7465092.1"
                     /translation="MAENPSRTVAALVDRARIAQSVVDRYDQSRTDEMALAAGWAIME
                     PARNRVLAEMAVRDTGCGNVDDKILKNYRKTLGLLRDLKGAKSVGIIAEYPERGLVEI
                     ARPVGVVAAITPSTNPAATPANKIINALKGRNAIVVAPSPKGHGTCVALIGYIHAELK
                     KVGAPLDLVQCLAAPVTREATQELMRQADLVVATGSQNNVRAAYTSGTPAFGVGAGNV
                     ASIVDSSANLAEAAAFIARSKTFDNATSCSSENSVVLLDDVFDGALAALQDAGGVLLD
                     PAEKQKLLETMWRGGKLSSETTAQSASRVAQLAGLTRAGIDKARFLMVAETGTGPDCP
                     FSGEKLAPVLTVYRARDFDHACRLVADIYSYQGAGHSVSIHTSNDEHIARLGLELPVC
                     RVIVNQVHCFATGGNFDNGLPFSLSMGCGTWGRNNFSDNLNYRHYLNITRIVRRIPES
                     VPAEDAIFGGYFQKYGA"
BASE COUNT          326 a          669 c          648 g          341 t
ORIGIN      
        1 cggaggtgta catcatcaac gcgacgtcct cttcgccgat gggagcgagg ggctcgccgt
       61 cgtccggaga ggcaggcagg aactcctcgg catcgacatc gcaggtcacc acctggatcg
      121 cccgctcgat cttctgcagc gcttcccgga ggcgaggctc ctggtcgggc gatgcgaaga
      181 tcagcgcgac atcgctgtga tcgagcacgt attcgaggtg cgacggctgc gccaggaggt
      241 tgaacggcgt cacgcaatag ccgccgtaca tggcgccgat gaaaaggcgg cacgcctggt
      301 agccgttgtg catcaggagg ccgaccttgt cgcccggctc gacatgccgc ccgcgcagga
      361 atgcggcaag ccgcagcgac gcgcttcgca gggaaccgaa gctcatggtg cgtcccgttt
      421 ccggcgcgac caggtaagtc gcgtcgggac gcgccttcgc ctgatggtcc acgcaatcgc
      481 gaatcacgcg ccgcatcggc tcacgcgccg tacttctgga agtagcctcc gaatatcgcg
      541 tcttcagcgg gcacgctctc cggaatccgg cgaacgatgc gcgtgatgtt gagatagtga
      601 cggtagttca ggttgtccga gaagttgttg cggccccacg tcccgcaacc catcgacagc
      661 gagaacggaa ggccgttgtc gaaattcccg ccggtcgcga agcaatgcac ctggttcacg
      721 atcaccctgc agaccggcag ctccagcccc agccgcgcga tgtgctcatc gttcgacgta
      781 tgaatgctca ccgagtgccc ggcgccctgg taggaataga tatcggcgac gagccggcag
      841 gcatggtcga aatcgcgcgc ccgatagacg gtgagcaccg gcgccagctt ttcacccgag
      901 aacgggcagt cgggcccggt gccggtttcg gcaaccatca ggaaacgcgc cttgtcgatg
      961 cccgcgcgcg tgagccccgc gagttgcgcg actctcgaag ccgactgcgc cgtagtctcc
     1021 gagctgagct tcccgccgcg ccacatcgtc tcgaggagct tctgcttttc cgcggggtcg
     1081 agcagcactc cgccagcatc ctgaagcgcc gccaaggccc cgtcgaaaac atcgtcgagc
     1141 agcaccacgc tgttttccga ggagcagctg gtggcgttgt cgaaggtctt cgagcgagcg
     1201 atgaaggccg cggcctccgc gaggttcgcc gagctgtcga cgatcgacgc cacgttgccc
     1261 gcgccgacgc cgaaggccgg cgtgccgctc gtgtatgcgg cgcgcacgtt gttctgcgag
     1321 ccggtcgcca cgacgagatc ggcctggcgc atcagctcct gcgtggcctc ccgggtcacc
     1381 ggcgccgcga ggcactgcac caggtccagg ggcgcgccca ccttcttcag ctccgcatgg
     1441 atgtagccga tcaacgcgac gcaagtgccg tgccccttgg gcgaaggcgc gacaacgatc
     1501 gcgttgcgcc ctttcagtgc attgattatc ttgttcgcgg gcgtcgccgc gggattcgtc
     1561 gacggagtga tcgccgcgac cacgccgacc ggccgcgcga tctcgacgag gccccgctcc
     1621 ggatattcgg cgatgatgcc cacgctcttc gcgcccttca ggtcgcgcag gagccccagc
     1681 gtcttgcggt agttcttgag gatcttgtcg tcgacgttgc cgcagcccgt atcgcgcacc
     1741 gccatctcgg cgagcacgcg gttgcgcgcc ggctccatga tcgcccaacc cgccgccagc
     1801 gccatctcgt cggtccgcga ctggtcgtag cggtccacga cggattgcgc gatgcgggcg
     1861 cgatcgacga gagcggcgac tgttcgcgaa gggttttccg ccatcgggct tctactcggg
     1921 gcggatgttg gcttcccgaa tgaggggagc ccacttcgcc agttccgcgg tgacgacgcg
     1981 cccg
//
LOCUS       DASVII010000042         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_74250, whole
            genome shotgun sequence.
ACCESSION   DASVII010000042 DASVII010000000
VERSION     DASVII010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74250"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF309_00530"
     CDS             complement(<1..237)
                     /locus_tag="VF309_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HEX7465093.1"
                     /translation="MIHFVVHDENDSVGVVVVEGVKAGQELTGWSMDQDKDLRVKAVS
                     DIPIGHKLAIKALGKDATVIKYGVDIGRVVAPIRV"
     gene            375..1097
                     /locus_tag="VF309_00535"
     CDS             375..1097
                     /locus_tag="VF309_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7465094.1"
                     /translation="MGHLYEQVRARLIEGISAGEWRAGAAIPTEAALAAGFGVAIGTV
                     RKAVDSLVAEQALVRRQGKGTFVTAHDGSRLLFHFFHIVARDGAKVYPEVRTVSFRRD
                     RADAAAAQALGIAPADKVIRIRNLLSLAGAAALVDDITLPAELYPGLTERIFLARGNT
                     IYHLYQSRYGINVLRTDERLRAVLAAREDAQLLAVPVGAPLLEIRRVALTFRDRPVEL
                     RLSRVNTARHDYHNTLGKGVAS"
     gene            1102..>1566
                     /locus_tag="VF309_00540"
     CDS             1102..>1566
                     /locus_tag="VF309_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7465095.1"
                     /translation="MQTALLILPNFVLIVIGLVLARRFDFGRDFWDGLEKLVYYVLFP
                     ALLFRSLVLARIDFGATGKLVLAALGFTLAGFALSLVAGPLFRLDRKLLATGSQCGYR
                     FNTYVGLAIAGSLFGTPGVALAALLLGVMIPLANFLAVAMLATHGKRGFLHEL"
BASE COUNT          214 a          582 c          487 g          283 t
ORIGIN      
        1 cacccggatg ggcgccacga cccgcccgat gtccacgccg tacttgatca cggtcgcgtc
       61 cttgccgagg gccttgatcg cgagcttgtg cccgatggga atgtcggaca cggccttcac
      121 gcgcaggtcc ttgtcctggt ccatgctcca gccggtgagc tcctggccgg ccttcacgcc
      181 ctcgaccacg acgacgccga ccgagtcgtt ctcgtcgtga accacgaaat gaatcatcga
      241 atcgctcctt gaattggatc ccggccggca ctttgattgg atccccgtcg gcacggggat
      301 gacctgcctt cggcgattct ccgaatccgc cacagccagg tcaactagat gacttggatg
      361 ttatcttccg ggcgatgggg catctctacg agcaggtccg cgcgcggctg atcgagggca
      421 tctccgccgg cgagtggcgc gcgggcgctg cgattcccac cgaggccgcg ctcgccgcgg
      481 gcttcggggt cgcgatcggc accgtgcgca aagccgtcga cagcctggtc gccgagcagg
      541 cgctggtgcg ccgccagggc aagggcacgt tcgtcacggc ccacgacggc agccgcctcc
      601 tcttccactt cttccacatc gtcgcgcgcg acggcgccaa ggtgtatccc gaggtgcgca
      661 cggtgtcgtt ccgccgcgat cgcgccgacg ccgccgccgc ccaggcgctt ggcatcgcgc
      721 ccgccgacaa ggtgatccgc atccgcaacc tgctctcgct cgcgggagcc gcggcgctcg
      781 tcgacgacat cacgcttccc gcggagctct accccggcct caccgagagg atcttcctcg
      841 cgcgcggcaa cacgatctac catctgtacc agagccgcta cggcatcaac gtgctgcgca
      901 ccgacgagcg actgcgcgcc gtgcttgccg cgcgtgaaga cgcgcagctg ctcgcggtgc
      961 cggtgggcgc gccgctcctc gagatccggc gcgtggccct caccttccgc gatcgcccgg
     1021 tggagctgag gctgtcgcgc gtgaacaccg cgcgccacga ctaccacaac accctcggca
     1081 agggcgtggc aagctgaagc gatgcagacc gcgctcctca tcctgcccaa cttcgtgctg
     1141 atcgtgatcg gtttggtgct cgcgcgccgc ttcgacttcg ggcgcgattt ctgggacggt
     1201 ctcgagaagc tcgtctatta cgtgctcttc ccggcgcttc tcttccgctc gctcgtgctg
     1261 gcgcgcatcg acttcggcgc gaccgggaag ctcgtcctcg ccgcgctcgg cttcacgctc
     1321 gcgggcttcg cgctctcgct cgtcgcggga ccgctcttcc ggctcgacag gaagctcctc
     1381 gccaccgggt cccaatgcgg ctatcgcttc aacacgtacg tgggcctcgc catcgccggg
     1441 agcctgttcg gcacgccggg cgtggcgctc gccgccctcc tcctcggcgt gatgatcccg
     1501 ctcgccaact tcctcgcggt ggcgatgctc gctacgcatg gcaagcgcgg tttcctgcac
     1561 gagctc
//
LOCUS       DASVII010000043         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_110284,
            whole genome shotgun sequence.
ACCESSION   DASVII010000043 DASVII010000000
VERSION     DASVII010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110284"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF309_00545"
     CDS             <1..197
                     /locus_tag="VF309_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7465096.1"
                     /translation="SASLFAPGNSIASALANEFTEAVGELYTSALIELGLLLFLITTI
                     VLALSKLLLLQLAKGEGTRT"
     gene            212..1042
                     /gene="pstA"
                     /locus_tag="VF309_00550"
     CDS             212..1042
                     /gene="pstA"
                     /locus_tag="VF309_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7465097.1"
                     /translation="MNPIYRRRKRMNSLMMGVSTAALAFGLFWLIWIVVVLLWEGASA
                     LRPSLFAQMTPPPGGDGGLANAIYGSVLMAGAGTLIGTPVGILAGTYLAEFGGRGWLA
                     PATRFINDVLLSAPSIVIGLFVYAIYVAQAKHFSGWAGAVALAIIVVPVVVRTTDDML
                     KLVPNSLREAAAALGAPSWKVVTMISYRAARAGIVTGVLLAIARITGETAPLLFTALN
                     NQFWSSDMNRPMANLPVVIFQFAMSPYEDWQRLAWGGATLITLFVLGLNIAARALFRK
                     "
     gene            1070..1852
                     /gene="pstB"
                     /locus_tag="VF309_00555"
     CDS             1070..1852
                     /gene="pstB"
                     /locus_tag="VF309_00555"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7465098.1"
                     /translation="MPEKVAEPARAAKLRVRNLNFYYGSFHALKGVSLEIPENRVTAF
                     IGPSGCGKSTLLRTFNRMFELYPEQRAEGEVLMGSENLLRSKMDVSLIRAKIGMVFQK
                     PTPFPMSIYDNIAFGVRLFEDLSRARMDERVEWALTKAALWKEAKDKLNQSGHSLSGG
                     QQQRLCIARGVAVRPEILLLDEPCSALDPISTAKIEELITELKVDYTVVIVTHNMQQA
                     ARVSDYTAYMYLGELIEFGETNQIFLKPGKQETEDYITGRFG"
     gene            complement(1865..2230)
                     /locus_tag="VF309_00560"
     CDS             complement(1865..2230)
                     /locus_tag="VF309_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465099.1"
                     /translation="MEYHLSLVPHPRYLHASVTGTHSAENAARFLREVREACASQGVS
                     AVLLEVKLSGPSLATGSIFNVISQESGWGRMLRKVAYVDASDRDPAKIKFAETVALNR
                     GVNVRLFASVEEAGRWLAQ"
     gene            complement(2230..2574)
                     /locus_tag="VF309_00565"
     CDS             complement(2230..2574)
                     /locus_tag="VF309_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7465100.1"
                     /translation="MPNTAVDVVQRQVDAYNARDLERFVAEFSDGVQVFRPPSTQPAI
                     SGKARLAEYYAANRFNLPALRADILNRIALGGRVIDHERIHGVRDEPFEVAAVYDVVD
                     GLIRTVWFFAAD"
     gene            2718..>2911
                     /locus_tag="VF309_00570"
     CDS             2718..>2911
                     /locus_tag="VF309_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465101.1"
                     /translation="MAAQARSMQPQATGFDAGIVRDEARISQARALRERVLARDNESR
                     PVHERFEEHSDHLVVRDAKS"
BASE COUNT          477 a         1010 c          936 g          488 t
ORIGIN      
        1 tttcggcctc gctcttcgcc ccgggcaact cgatcgcctc cgctcttgcg aacgagttca
       61 ccgaggcggt cggcgagctc tacacctcgg cgttgatcga gctggggctt ctcctcttcc
      121 tcatcacgac catcgtgctc gcgctgtcga agctcctgct gctgcagctc gccaagggcg
      181 aagggacgcg cacgtgaacc cggtctcggc gatgaatccc atctaccggc gccgcaagcg
      241 catgaactcg ctcatgatgg gcgtgtcgac cgcggccctc gcgttcggcc tcttctggct
      301 gatatggatc gtcgtggtgc tgctctggga aggcgcttcg gcgctgcgcc cctcgctctt
      361 cgcccagatg acgccgcccc ccggcggcga cggcggcctc gccaacgcga tctacggcag
      421 cgtgctcatg gccggcgcgg gaaccctcat cggcacgccg gtcggcatcc tcgccgggac
      481 ctacctcgcg gaattcggcg gccgcggctg gctcgcgccg gcgacgcggt tcatcaacga
      541 cgtgctgctg tccgcgccct cgatcgtgat cggcctcttc gtttacgcga tctacgtcgc
      601 gcaagccaag catttctccg ggtgggccgg agccgtcgcg ctcgcgatca tcgtggtccc
      661 ggtcgtggtg cgcaccaccg acgacatgct caaactcgtg cccaacagcc tgcgcgaagc
      721 ggccgccgcg ctcggcgcgc ccagctggaa ggtggtcacg atgatctcct atcgcgccgc
      781 gcgcgcgggc atcgtcaccg gcgtcctgct cgcgatcgcg cgcatcacgg gcgagaccgc
      841 gccgctcctc ttcaccgccc tcaacaacca gttctggtcg tccgacatga accggcccat
      901 ggccaacctc ccggtggtca tcttccagtt cgcgatgagc ccctacgagg actggcagcg
      961 gctcgcctgg ggcggcgcca ccctcatcac gctcttcgtg ctcgggctca acatcgccgc
     1021 gcgcgcgctc ttcaggaagt agccgatgcc cgcctccgtg atgcacgcca tgccggaaaa
     1081 ggtcgcggag cccgcgcggg ccgcgaagct gagggtgcgc aacctcaatt tctactacgg
     1141 gtccttccac gcattgaagg gcgtgagcct cgagatcccg gagaatcgcg tgacggcgtt
     1201 catcggcccg tcgggatgcg gcaagtcgac gctgctgcgc accttcaatc gcatgttcga
     1261 gctctacccc gagcagcgcg ccgaaggcga agtgttgatg ggtagcgaga acctcctgcg
     1321 ctcgaagatg gatgtctcgc tcatccgcgc caagatcggc atggtgttcc agaagcccac
     1381 gccctttccg atgtcgatct acgacaacat cgccttcggc gtgcggctct tcgaggacct
     1441 ttcccgcgcg cgcatggacg agcgcgtcga gtgggcgctc accaaggccg cgctgtggaa
     1501 ggaggcgaag gacaagctga accagagcgg ccacagcctc tcgggcggcc agcagcagcg
     1561 cctgtgcatc gcgcgcggcg tggccgtgcg tcccgagatc ctgctgctcg acgagccctg
     1621 ctcggcgctg gacccgatct cgacggcgaa gatcgaggag ctcatcaccg agctcaaggt
     1681 ggactacacc gtggtaatcg tgacgcacaa catgcagcag gcggcgcgcg tttccgacta
     1741 caccgcgtac atgtacctgg gcgagctgat cgagttcggc gagaccaacc agatcttcct
     1801 gaagccgggc aagcaggaga ccgaggacta catcaccggt cgcttcggct aggctcggtc
     1861 gcgctcactg cgcaagccat cgcccggcct cctcgacgct cgcgaacaat cgaacgttga
     1921 cgccgcggtt cagtgcgacc gtctcggcga acttgatctt cgcggggtcg cgatccgagg
     1981 cgtcgacata ggcgaccttg cgcagcatcc ttccccaccc gctttcctgc gagatcacgt
     2041 tgaagatgct tccggtggcg aggctcggcc ccgagagctt cacctcgagg aggaccgccg
     2101 agacgccctg gctcgcgcac gcctcgcgga cttcgcgcag gaacctcgcc gcgttctccg
     2161 cgctgtgggt cccggtgacc gacgcatgca ggtagcgggg atggggcacg aggctgaggt
     2221 ggtattccat caatcggccg cgaagaacca gacggtccgg atgaggccgt ccacgacgtc
     2281 gtagaccgcc gcgacctcga acggctcatc gcgcaccccg tggatgcgct cgtggtcgat
     2341 cacgcggccg ccgagcgcga tgcgattgag aatgtcggcc cgaagcgcgg gaaggttgaa
     2401 ccgattcgcc gcgtagtact ccgcgagccg ggccttgccc gagatcgcgg gctgcgtcga
     2461 gggcggccgg aacacctgca ccccgtcgct gaactccgcc acgaatcgct ccaggtcgcg
     2521 cgcgttgtac gcatcgacct ggcgctggac gacatcgacg gcggtgttgg gcatttgcag
     2581 ggtccctgtt caacgagatg gcgggtgttg ccggaaattc tccggttcca cggcgcgcgg
     2641 gtcaaacgga gccgtcaccc cactgtcatg ggcctgtcac gcagggttcc taagctctgg
     2701 ggcaacgagg aggaccgatg gcagcgcagg cgcgaagcat gcagccgcaa gcgacgggct
     2761 tcgacgcggg aatcgtccgc gacgaggcgc gcatctccca ggcgcgagcc cttcgcgaaa
     2821 gggtcctcgc gcgagacaac gaatcgcggc ccgtccacga acgcttcgag gagcacagcg
     2881 accatctcgt cgtgcgcgac gcgaagtccg a
//
LOCUS       DASVII010000044         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_110299,
            whole genome shotgun sequence.
ACCESSION   DASVII010000044 DASVII010000000
VERSION     DASVII010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110299"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VF309_00575"
     CDS             <1..487
                     /locus_tag="VF309_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7465102.1"
                     /translation="RRLALLAASFAGIALTLGGGHGTAEGIALGLGAAALYSVYIVVG
                     ARELANADPVASGTIVCLSAAATLLAVSLFREPHFPGVWWSWAALAAIALVSTVIAIM
                     AFFAGLRRVGPAMASIASTLEPVVTVALAWTLLGESLSPIQIAGGALVLAAAASLAVR
                     A"
     gene            596..901
                     /locus_tag="VF309_00580"
     CDS             596..901
                     /locus_tag="VF309_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465103.1"
                     /translation="MYACPWCERKVFSFWQKQSLGPSKTLQCTGCKRRVSVSWLHAHL
                     AALPMFVFAICGLWFVGDAFNSRIFALAGALGGVVLGMMVMMPLYHYIVPLVKPDRR"
     gene            complement(945..>1803)
                     /locus_tag="VF309_00585"
     CDS             complement(945..>1803)
                     /locus_tag="VF309_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEX7465104.1"
                     /translation="GRTFPFEVWVNGAEQPRGLGAVAKTLSMDMRADDREWLKMKLDV
                     LASTVGDDSFDMQFPPHGEKRRVPGVVSAVAQAVRWRCEQLNTFDSPGATPVLDHMFS
                     IDEPRTGADGTLSWTVDIANPASGDEFVLGLKEVHLPDGATRPYSIWLSGHYPRALDG
                     LTRILSLDMRVVDPAWIGMKLRKLLDYSEPLGDFMAFVPGERRQQNWPSTVAYLARLV
                     IHRYAMLGVLDERGYPTREMGVLEAPRAAGDPAVMKGARCRECGNYTVIRKDGCDFCS
                     ACGWTGMCG"
BASE COUNT          263 a          603 c          633 g          304 t
ORIGIN      
        1 caggcgcctc gcgttgctcg cggcgagctt cgcggggatc gcgctcacgc tgggcggcgg
       61 ccacggcacc gccgagggca tcgcgctggg gctcggcgcc gcggcgctct attcggtcta
      121 catcgtggtc ggcgcgcgcg agctcgcgaa cgccgatccc gtggcgagcg gcacgatcgt
      181 gtgcctctcc gcggccgcga cgcttctcgc cgtgagcctc tttcgcgagc cgcactttcc
      241 gggcgtgtgg tggagctggg cggcgctcgc cgcgatcgcg ctcgtgtcca cggtgatagc
      301 gatcatggct ttcttcgcgg gactcaggcg cgtcggcccc gcgatggcct ccatcgcttc
      361 gacgctcgag ccggtggtca cggtcgcgct cgcgtggacg ctgctgggcg agtcgctctc
      421 gccgatccag atcgcgggag gggccctggt gctcgccgct gccgcctcgc tcgccgtcag
      481 ggcctgagcg caaggaactc ccggcgggtt acaaaggcaa ttttggaagc ggattgatat
      541 cggggaaaat gagcttcgag ccgcgttcca tggcggacgc ttcagggagt caccgatgta
      601 cgcatgtccg tggtgcgagc gcaaagtctt ctcgttctgg cagaagcaat cgctcggtcc
      661 gagcaagacg ctgcaatgca cgggatgcaa gcggcgcgtg agcgtctcgt ggctgcatgc
      721 gcatctcgcg gcgctcccga tgttcgtgtt cgcgatatgc gggctgtggt tcgtcggcga
      781 cgcgttcaac tccaggatct tcgcgctcgc gggcgcgctc ggcggcgtgg tgctcggcat
      841 gatggtcatg atgccgctct accactacat cgtgccgctg gtgaagccgg atcgccgcta
      901 gagaataggc gatccgtcct cgcgaaggcg gggacccaga cagctcagcc gcacattccg
      961 gtccagccgc aagcgctgca gaaatcgcag ccgtccttgc ggatgacggt gtagttgccg
     1021 cactcgcggc agcgcgcgcc tttcatgacc gccgggtcgc cggccgcccg gggcgcctcg
     1081 aggacgccca tctcgcgcgt cggatagccg cgctcgtcga gcacgcccag catcgcgtag
     1141 cggtggatca cgaggcgcgc gaggtacgcg accgtcgaag gccagttctg ctggcggcgc
     1201 tcgccgggaa cgaacgccat gaaatcgccg aggggctccg agtaatcgag gagcttgcgc
     1261 agcttcatgc cgatccatgc ggggtcgacc acgcgcatgt cgagcgagag gatgcgcgtg
     1321 aggccgtcga gcgcccgcgg gtagtggccc gagagccaga tcgaataggg acgcgtcgcg
     1381 ccgtcgggaa ggtgcacctc cttcagtccc agcacgaatt cgtcgcccga agccggattc
     1441 gcgatgtcga cggtccacga gagcgtgccg tcggcgccgg tgcgcggctc gtcgatgctg
     1501 aacatgtggt ccaggaccgg cgtcgcgccc ggggaatcga acgtgttcag ctgctcgcag
     1561 cgccagcgca ccgcctgcgc caccgccgag accacgccgg gcacgcggcg cttctcgccg
     1621 tgcggtggaa attgcatgtc gaacgaatcg tcgcccacgg tgctcgcgag gacgtccagc
     1681 ttcatcttga gccattcgcg gtcgtcggcg cgcatgtcca tcgagagggt cttcgcgacg
     1741 gcacccaggc cccgcggctg ctccgcgccg ttgacccaca cctcgaacgg gaaggtgcgc
     1801 ccc
//
LOCUS       DASVII010000045         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_218085,
            whole genome shotgun sequence.
ACCESSION   DASVII010000045 DASVII010000000
VERSION     DASVII010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218085"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1618
                     /locus_tag="VF309_00590"
     CDS             56..1618
                     /locus_tag="VF309_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX7465105.1"
                     /translation="MLRFDNTFVSRLPGDPLTGSQRRQVHGALFSQIEPTPVRAPRLI
                     AHSREVAAMLGFDERYVASPEFAQVFGGNALLEGMRPYAANYGGHQFGNWAGQLGDGR
                     AITLGETVNAKGERWELQLKGAGPTPYARTADGRAVLRSSVREFLCSEAMHHLGIATT
                     RALSLVATGEQVERDMFYDGNPKLEPGAIVCRVAPSFIRFGNFELPASRGDTALLTQL
                     IDFTIARDFPELAPGGLEGEALRGEWFGQVCERTARMVADWMRVGFVHGVMNTDNMSI
                     LGLTIDYGPYGWIDNFDLEWTPNTTDAGGRRYRFGHQPHVAHWNLGRLANALAPAFAS
                     HDSLYAGLDRFVAAFNEADRAAIAAKLGLAACGDEDIDLIQDLHGLLQEAEVDMTIFF
                     RALSRVDLAVPSVEPMADAFYDEGKRHAAEPRFNEWLARYAARLREDSLSREERRRKM
                     DAANPRYVLRNYLAQQAIDRAEQGDDAGIHELLELLRRPYEEQPGREAFAKRRPDWAR
                     DRAGCSMLSCSS"
BASE COUNT          263 a          625 c          591 g          278 t
ORIGIN      
        1 acctccgggc gcgagttatc cttgaaaatt cgtacctgac accaatttgc catccatgct
       61 gcgtttcgac aacaccttcg tctcgcgcct tcccggcgat ccgctgaccg gatcgcagcg
      121 ccgccaggtg catggcgccc tcttctcgca aatcgagccc acgcccgtgc gcgcgccgcg
      181 gctcatcgcg cactcgcgcg aggtcgcggc gatgctcggc ttcgacgagc gctacgtcgc
      241 ctcgcccgag ttcgcgcagg tgttcggcgg caacgcgctc ctcgagggaa tgcggcccta
      301 tgcggcgaac tacggcggcc accagttcgg caactgggcg gggcagctcg gcgacgggcg
      361 cgcgatcacg ctcggcgaga cggtgaacgc gaagggcgag cgctgggagc tgcagctgaa
      421 gggcgcgggc cccacgccct atgcgcgcac cgccgatgga cgcgccgtgc tgcgctcctc
      481 ggtgcgcgag ttcctgtgca gcgaggcgat gcaccacctc gggatcgcga ccacgcgcgc
      541 gctgagcctc gtcgccaccg gcgagcaggt cgagcgcgac atgttctacg acggcaatcc
      601 gaagctcgag cccggcgcga tcgtctgccg cgtcgcgcct tccttcatcc gcttcggcaa
      661 cttcgagctt cccgcctcgc gcggcgacac ggcgctcctc acgcagctca tcgacttcac
      721 catcgcccgc gattttcccg agctcgcgcc gggcggcctc gagggcgagg cgctgcgcgg
      781 cgagtggttc gggcaggtgt gcgagcgcac ggcgcgcatg gtggccgact ggatgcgcgt
      841 gggattcgtc cacggcgtga tgaacacgga caacatgtcg atcctcgggc tcacgatcga
      901 ctacggcccc tatggctgga tcgacaactt cgacctcgag tggacgccca acaccaccga
      961 cgcggggggg cggcgctatc gcttcgggca ccagccccac gtggcgcact ggaacctggg
     1021 tcgcctcgcg aacgcgctcg cgcccgcatt cgcatcccat gattcgttgt acgcggggct
     1081 cgaccggttc gtcgcggcct tcaacgaagc cgatcgcgcc gcgatcgcgg cgaagctcgg
     1141 gctcgcggcg tgcggtgacg aggacatcga tctcatccag gacctccacg ggctgctgca
     1201 ggaagccgag gtcgacatga cgatcttctt ccgcgcgttg agccgggtgg atctcgcggt
     1261 cccgagcgtc gagcccatgg cggacgcctt ctacgacgag ggcaagcgcc acgccgccga
     1321 gccgcgcttc aacgagtggc tcgcgagata cgccgcgcgc ctgcgcgagg attctctttc
     1381 acgggaggaa cgccgccgca agatggatgc cgcgaatccc cgctacgtgc tgcgcaatta
     1441 cctcgcgcaa caggcgatcg atcgcgccga gcagggcgac gacgccggca tccacgagct
     1501 gctcgagctg ctgcgccgcc cctacgaaga gcagccggga cgcgaagcgt tcgcgaagcg
     1561 ccgtcccgat tgggcgcgag accgcgcggg ttgctcgatg ctttcgtgca gctcctaggc
     1621 cgcgccgcgg ttcatccgcc gggccgggcc atgcagtccg aataggcctg gcgccgggca
     1681 aggttggtct cccggacggc ctgcgcctct tcgggcgcgg gaagcttgag cgtggtcttc
     1741 agctccgcct tggtccc
//
LOCUS       DASVII010000046         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_505830,
            whole genome shotgun sequence.
ACCESSION   DASVII010000046 DASVII010000000
VERSION     DASVII010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505830"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF309_00595"
     CDS             <1..328
                     /locus_tag="VF309_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465106.1"
                     /translation="SLSERERATLAAYLAVNMPVAADAARGDVAAALPPDGRYLAENE
                     CQSCHSIFSSYLTQSRDAQGWRNVFLSPFHKQLKMAPTQREEFARYSALNMPMKVDEV
                     PQDLRF"
     gene            331..921
                     /locus_tag="VF309_00600"
     CDS             331..921
                     /locus_tag="VF309_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7465107.1"
                     /translation="MKSPRIRALILAFAGLAALAAAAPEKDVFEFVPEGGRTLLAKAF
                     AGRVPAEDVRAIIGGKHTSVEWVADLRAREKSLPALKRLDDKERRTLADYLAANMPLA
                     KPPADPAKASWDKALPLDGRDMALNYCQGCHIITVVITQDRSKTAWLGTMGKPSHVQI
                     KLTPAQRDALANYLIVNAAIPIDQVPEELRAGGATY"
     gene            943..>1577
                     /locus_tag="VF309_00605"
     CDS             943..>1577
                     /locus_tag="VF309_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465108.1"
                     /translation="MLFTSLEFFLFLPLAAALYAALPARHRWLALLAASYVFYGAARP
                     ANLVYLGGVTLVVLACGAALERLERETARRAVLAAGLVAALGSLAAFKFYDFLAGELE
                     RAAGVSLPRMGLTAPAGYSFYAFSAASYLIDSFRRLLPRPFGAGHVAAYLAHFPKILA
                     GPIDRATNFLPQLAAGARATPEQLVLGSQLILWGLVKKVLVADNLAPLVDHA"
BASE COUNT          222 a          607 c          487 g          261 t
ORIGIN      
        1 ctctctctcg gagcgcgagc gcgcgacgct cgccgcctac ctcgcggtga acatgcccgt
       61 tgcggccgat gccgcgcgcg gcgacgtcgc ggcggcgctt ccccccgacg ggcgctacct
      121 cgccgagaac gagtgccagt cttgccactc gatcttttcg agctacctca cccaatcgcg
      181 cgacgcgcag ggctggcgca acgtctttct ctcgcctttc cacaagcagt tgaagatggc
      241 gcccacgcag cgcgaggaat tcgcgcgcta ctcggcgctc aacatgccga tgaaggtcga
      301 cgaagtgccg caggacttgc gcttctgacc atgaaatcgc cacgaatccg cgcgctgatc
      361 ctcgccttcg cggggctagc ggcgctcgct gcggccgcgc cggagaagga tgtcttcgag
      421 ttcgtcccgg aaggcggccg cacgctgctc gccaaggcct tcgccggccg cgttcccgcg
      481 gaagacgtcc gcgcgatcat cggagggaag cacacttccg tcgaatgggt cgccgacctg
      541 cgcgcgcgcg agaagtcgct gcccgcgctg aagcgcctcg acgacaagga gcggcgcacc
      601 ctcgccgact atctcgctgc gaacatgccg ctcgccaagc cacccgcgga tcccgcgaag
      661 gcgagctggg acaaggcgct gccgctggac gggcgcgaca tggcgctcaa ctattgccag
      721 ggctgccaca tcatcaccgt cgtcatcacc caggaccgct cgaagaccgc atggctcgga
      781 acgatgggca agcccagcca cgtgcagatc aagctcaccc ccgcgcagcg cgacgcgctc
      841 gcgaactatc tcatcgtcaa cgccgcgata ccgatcgacc aggtgcccga ggagctgcgc
      901 gccggcggcg cgacgtactg accgggcgcg gagcgcgccc ccttgctctt cacctcgctc
      961 gagttcttcc tcttcctgcc gctagcagct gcgctgtatg cggcgctccc ggcgcgtcac
     1021 cgctggctcg ccctcctcgc cgcgagctac gtcttctatg gtgcggcccg ccccgcgaac
     1081 ctcgtctacc tcggcggcgt gacgctggtg gtgctcgcct gcggcgcggc cctggagcgc
     1141 ctggagcgcg aaacggcgcg gcgcgcggtt ctcgcggcgg ggctcgtcgc ggcgctgggt
     1201 tccctcgccg cgttcaagtt ctacgacttt ctcgccggcg agctggaacg cgccgccggc
     1261 gtctcgcttc cccgcatggg gctcacggcg cccgccggct attccttcta cgccttctcc
     1321 gcggcgagct atctcatcga ttcctttcgc cgcctgcttc cacggccgtt cggcgcgggc
     1381 catgtcgccg cctacctcgc ccatttcccg aagattctcg ccggcccgat cgaccgcgcc
     1441 accaacttcc tcccgcaact cgccgcgggg gcgcgcgcga ccccggagca gctcgtcctc
     1501 ggctcccagc tcatcctctg gggcctggta aagaaggtcc tcgtcgccga caatctcgcg
     1561 ccgctcgtgg accatgc
//
LOCUS       DASVII010000047         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_362232,
            whole genome shotgun sequence.
ACCESSION   DASVII010000047 DASVII010000000
VERSION     DASVII010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362232"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF309_00610"
     CDS             complement(<1..442)
                     /locus_tag="VF309_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7465109.1"
                     /translation="MHSIRIDSGPEFHCEADDTLLRAGLRAGLGLPYECNAGSCGTCK
                     VELVDGQVTSLRPEAPGLTDRDRAKNRVLACQAQPGTDCRIKVRLREENVPRHKPARF
                     AATLTSFRDLTHDMREFTFTAKDSPGFLPGQYALFYLPGLEAPRT"
     gene            complement(442..1254)
                     /locus_tag="VF309_00615"
     CDS             complement(442..1254)
                     /locus_tag="VF309_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7465110.1"
                     /translation="MERVKKPKARRVRLSSVANSIRLTKAFSEDEYEMGISALATRLG
                     LAKSTVHRLATTLVEYDILEQNRETGKYRLGLALFELGTMVRRKMDTNSGAQVQIHAL
                     ADETGETVQLAILDHLSVLYIRIRESRQAVRMSSGLGSRAPAHCTGVGKALIAFQPPE
                     IVQQIIDNGLKRYTQGTITDPEKLRAELATIRQKGYAIDDEEIEVGLRCIAAPIRDHS
                     GQVTAAISVAAPVQRMTKKNLQATIPSVVAAAEAISRRLGYLPSLAGTLMAD"
     gene            complement(1258..>1557)
                     /locus_tag="VF309_00620"
     CDS             complement(1258..>1557)
                     /locus_tag="VF309_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HEX7465111.1"
                     /translation="ATILGLVFTAGMMATDALNGLWVSQLVRSADRRAAAASRTMSLA
                     IGFTSLAIAALAAARYVLPALDERVASWGIALSAGVVVAVAASYAIAMRLTGQEA"
BASE COUNT          234 a          530 c          538 g          255 t
ORIGIN      
        1 aggtgcgcgg cgcctcgagc cccggaaggt agaagagcgc gtactgcccc ggaagaaagc
       61 ccggcgaatc cttcgcggtg aacgtgaatt cgcgcatgtc gtgggtgaga tcgcgaaagc
      121 tcgtgagcgt cgcggcgaac cgcgccggct tgtggcgcgg aacgttctcc tcgcgaagcc
      181 gcaccttgat cctgcagtcc gttcccggct gcgcctggca ggcaagcacg cggttcttcg
      241 cgcggtcgcg atcggtcaat ccgggcgctt cgggacgcag cgacgtcacc tgcccatcga
      301 ccagctcgac cttgcacgtg ccgcacgatc cggcgttgca ctcgtaggga agccccagcc
      361 ccgcgcgaag gcccgcgcgc agcagcgtat cgtccgcttc gcagtggaac tcggggccgc
      421 tgtcgatgcg gatggaatgc atcaatccgc catcagcgtg ccggcaagcg atggaaggta
      481 gcccagccgc cgcgagatcg cctcggcggc ggcgacgacc gaggggatgg tcgcctggag
      541 gttcttcttc gtcatgcgct gcaccggggc cgcgacgctg atcgccgccg tcacctggcc
      601 cgagtggtcg cggatcggag ctgcgatgca gcgcaggccc acctcgatct cctcgtcgtc
      661 gatcgcgtag cccttctggc ggatggtcgc gagctcggcg cgcagcttct cgggatcggt
      721 gatggtgcct tgcgtgtagc gcttcaggcc gttgtcgatg atctgctgca cgatctcggg
      781 cggctggaac gcgatgagcg ccttgccgac gccagtgcag tgcgcgggcg cgcgtgatcc
      841 gagccccgac gacatccgta ccgcctggcg gctctcgcga atgcggatgt agagcacgct
      901 caggtgatcg aggatcgcga gctgcaccgt ctcgccggtc tcgtccgcga gcgcgtggat
      961 ctgcacttgc gcgcccgagt tggtgtccat cttgcgccgc accatcgtcc ccagctcgaa
     1021 gagggcgagg ccgaggcgat actttccggt ctcgcggttc tgctcgagga tgtcgtactc
     1081 gacgagcgtc gtcgcgaggc gatgcacggt gctcttcgcg agcccgaggc gcgtcgcgag
     1141 cgcgctgatc cccatctcgt attcatcctc ggagaacgcc ttggtgaggc gaatcgagtt
     1201 cgccacagag gagaggcgca cgcgtcgcgc cttgggcttc ttcacgcgtt ccatcggcta
     1261 ggcctcctgc cccgtgagcc gcatcgcgat cgcgtaggag gcggcgaccg cgacgaccac
     1321 gcccgcgctc aatgcgattc cccacgacgc cacccgctcg tcgagcgccg gcagcacgta
     1381 gcgcgccgcc gccagcgccg cgatcgcgag ggacgtgaag ccgatggcga ggctcatggt
     1441 gcgcgaggcg gccgcggcac gccggtccgc cgaacgcacc agctggctca cccacagccc
     1501 gttcagcgca tcggtggcca tcatgccggc ggtgaagacg aggccgagga tcgtggc
//
LOCUS       DASVII010000048         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_254262,
            whole genome shotgun sequence.
ACCESSION   DASVII010000048 DASVII010000000
VERSION     DASVII010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254262"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1865
                     /locus_tag="VF309_00625"
     CDS             <1..>1865
                     /locus_tag="VF309_00625"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7465112.1"
                     /translation="VATTRPETMLGDVAVAVHPEDERYSKLVGKNVKLPLADRLIPVI
                     ADTYVDREFGTGVVKITPAHDFNDYQVGVRHGLPMIDIFTLDARVNDNAPIAYRGLDR
                     FEARKRVLEDLEKLGLVESAKPHKLTVPRSERSDAIVEPMLSDQWFVRMDGMAKAGLE
                     AVSKGDTRFVPEEWTKVYNQWLGNIQDWCISRQLWWGHQIPAWYADDGSPYVGRTFQE
                     AAKRASAAGKAIAPHTRDPDVLDTWFSSGILPFTTLGWPDEARFEKERAFYLPSTVLV
                     TGFDIIFFWVARMIMTTLEFTGVTAFKDVYINALVRDAEGQKMSKSKGNTIDPLDVID
                     GIEFAPLLEKSTQGLMLASHKESASKRIKREFPEGIPSYGADSLRFTFASLSTLGRTL
                     NFDLKRCEGYRSFCNKLWNATRFVLMNTEGKDVGLDESAPLEFSKADRWIASRLQRAE
                     KAFIDGLAEYRFDVSARAVYEFVWDEYCDWYVELAKVQLATGNEAQQRATRRTLIRVL
                     EATLRLAHPIIPFITEELWQTVGPLAGKSGKSIMVAPFPKPDEKRIDEEAEKYALALK
                     ESIDAARNLRAANNVSPADKLDLYVPAAANFTADDHGYFRALARSSTIHVGSPAPG"
BASE COUNT          343 a          644 c          575 g          303 t
ORIGIN      
        1 gtcgccacca cgcgtcccga gacgatgctg ggcgatgtcg ccgtcgcggt gcatcccgag
       61 gacgagcgct acagcaaact cgtcgggaag aatgtgaagc ttccccttgc cgatcgcctg
      121 attccggtga tcgccgatac ctacgtcgat cgcgagttcg gcaccggggt cgtgaagatc
      181 acgcccgccc acgacttcaa cgattaccag gtgggcgtgc gtcacggcct gccgatgatc
      241 gacatcttca cgctcgacgc acgcgtgaac gacaacgcgc cgattgccta tcgcggcctc
      301 gatcgcttcg aggcgaggaa acgcgtgctg gaggatctcg agaaactcgg cctcgtcgag
      361 agcgccaagc cccacaagct cacggtgccg cgctcggagc gctccgacgc catcgtggag
      421 cccatgctct cggaccagtg gttcgtgcgc atggacggca tggcgaaggc ggggctcgag
      481 gccgtaagca agggcgacac gcgcttcgtc cccgaggaat ggaccaaggt ctacaaccag
      541 tggctcggga acatccagga ctggtgcatc tcgcgccagc tctggtgggg acaccagatt
      601 cctgcctggt acgccgacga cggctcgccc tacgtgggtc gcacgttcca ggaggcagcc
      661 aagcgcgcga gcgcggccgg caaggcgatc gctccgcaca cgcgcgatcc cgacgtgctc
      721 gatacctggt tctcgtcggg gatcctcccc ttcacgacgc tcggctggcc cgacgaggcg
      781 cgcttcgaga aggagcgcgc cttctacctg ccgtccacgg tgctcgtcac cggcttcgac
      841 atcatcttct tctgggtcgc gcggatgatc atgacgacgc tggaattcac cggcgtcacc
      901 gcgttcaagg acgtctacat caacgccctg gtgcgcgacg ccgagggcca gaagatgtcg
      961 aagtcgaagg gcaacaccat cgacccgctc gacgtgatcg acggcatcga attcgcgccg
     1021 ctcctcgaga agtcgacgca gggcctgatg ctcgcctccc acaaggagtc ggcctcgaag
     1081 cgcatcaagc gcgagttccc cgagggcatt ccctcctacg gcgccgactc gctgcgcttc
     1141 accttcgcct cgctctcgac gctcggccgc acgctcaact tcgatctcaa gcgctgcgag
     1201 ggctaccgca gcttctgcaa caagctctgg aacgcgacgc gcttcgtcct catgaatacc
     1261 gagggcaagg acgtcggcct cgacgaatcc gcgccgctgg aattctccaa ggccgatcgc
     1321 tggatcgcga gccgcctgca gcgcgccgag aaggccttca tcgacggcct cgccgagtat
     1381 cgcttcgacg tctccgcgcg cgccgtgtac gaattcgtgt gggacgagta ttgcgactgg
     1441 tacgtcgagc tcgcgaaggt gcagctcgcc accggcaacg aagcccagca gcgcgcgacc
     1501 cggcggacct tgatccgcgt gctcgaggcg acgctgcgcc tcgcacatcc gatcattccc
     1561 ttcatcaccg aggagctgtg gcagaccgtg ggcccgctcg ccggtaaatc gggcaagagc
     1621 atcatggtcg cgcccttccc caagcccgac gagaagcgca tcgacgaaga ggccgagaaa
     1681 tacgcgctcg cgctcaagga atccatcgac gccgctcgca acctgcgcgc cgcgaacaat
     1741 gtcagccccg ccgacaagct cgatctctac gtgccggccg cggcgaactt cacggccgac
     1801 gatcatggat atttcagggc gctggcgcgg tcatcgacga tccacgtggg atctccggcg
     1861 ccggg
//
LOCUS       DASVII010000049         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_2591, whole
            genome shotgun sequence.
ACCESSION   DASVII010000049 DASVII010000000
VERSION     DASVII010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..>1515
                     /gene="dnaA"
                     /locus_tag="VF309_00630"
     CDS             218..>1515
                     /gene="dnaA"
                     /locus_tag="VF309_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7465113.1"
                     /translation="MNELWSEAVRSIEPMVKPAHRELWLRPIECVGISDGRIRLRAPN
                     RYHKEWFEDNFLPPILRDLEIRAQRSFTVDFEVMGEQGGEAGPHPAPAHLRGPGLPVP
                     APPPNLVTRYTFDKFVVGPTNQFAHAAARMVAEAPASRWNPLFIYGGVGLGKTHLLQA
                     IGHEIHRQHPDWVITFVTCEGFVTDFIGSMMNQRRQSGTNLMEEFRTRYREYPDVLLI
                     DDIQFLSNKDSSQDEFFHTFNALHYAHKQIVLTSDKLPAELPGIEDRLRSRFTWGLIG
                     EVEMPDLETRVAILKRKAEFEKVILPDDVALYMAAHVKTNVRELEGALLRVAARASFQ
                     AQAITLDVARDALQKLLANTPTGLTIESIQREVAAYFDIKLHDLKGPKRHRAVAHPRM
                     IAMYLARKLTSMSFPEIGSRFGGKDHSTVISAVRKIERLCA"
BASE COUNT          283 a          492 c          481 g          259 t
ORIGIN      
        1 ggcgttcgaa gcggtctgat agagaagcgc tcccgctcac gagttttcgt cgcgagctcc
       61 agcactcggg gaaatctttc acgcttctcg ccgcgcgcct gcgcggcgga agaacagatc
      121 ttgtcgttgg ctgttgatag agaggggaaa gtccagtgaa agagctcgtc atgcataagg
      181 attatccgcg cgttgccctg tggataggtt gccaaacatg aatgagctct ggtcagaagc
      241 ggtccgctcg atcgagccga tggtcaagcc ggcgcatcgt gagctttggc tgcgtcccat
      301 cgagtgcgtg gggatcagcg acggacggat ccggctgcgc gcgccgaacc gctatcacaa
      361 ggaatggttc gaggataact tcctgccgcc gatcctcaga gatctggaga tacgcgctca
      421 gcgtagcttc accgtcgact tcgaggtgat gggtgagcaa gggggcgagg caggaccgca
      481 tccggcgccg gcccacctgc gcgggcccgg gcttcccgtc cccgcgccgc cgccgaatct
      541 ggtcacccga tacaccttcg acaagttcgt cgtcggtccc accaaccagt tcgcgcacgc
      601 cgccgcccgc atggtcgcgg aggctccggc ctcgcgctgg aatccgttgt tcatctacgg
      661 cggcgtcggc ctgggaaaga cccatctgct gcaagccatc ggtcacgaga ttcaccggca
      721 gcaccccgac tgggtcatca ccttcgtgac ctgcgagggc ttcgtcaccg acttcatcgg
      781 atcgatgatg aatcagcggc ggcagagcgg gacgaacctc atggaggagt tccgcacccg
      841 ctaccgcgag tacccggacg tgctgctcat cgacgacatc cagttccttt cgaacaagga
      901 cagctcgcag gacgagttct ttcacacctt caacgcgctt cactacgcgc acaagcagat
      961 cgtcctgacc tccgacaagc tgccggcgga gctgccgggg atcgaggatc ggctgcgcag
     1021 ccggttcacc tggggcctca tcggcgaggt cgagatgccc gacctcgaga cccgggtcgc
     1081 gatcttgaag cgcaaggcgg agttcgagaa ggtgatcctg cccgacgacg tcgccctcta
     1141 catggcagcg cacgtgaaga ccaacgtgcg ggagctcgag ggcgcgttgc tgcgggtggc
     1201 ggcgcgggcc tccttccagg cccaggccat cacgctggac gtcgcgcggg acgccctgca
     1261 gaagctgctg gccaacacgc cgaccggcct caccatcgag agcatccagc gggaggtcgc
     1321 ggcctacttc gacatcaagc tgcacgatct caagggaccg aagcgccatc gcgccgtcgc
     1381 gcacccgcgg atgatcgcga tgtacctggc gcgaaagctc acctcgatga gcttccccga
     1441 gatcgggagt cgcttcggcg gcaaggacca ctcgacggtc atcagcgccg ttcgcaagat
     1501 cgagcggctg tgcgc
//
LOCUS       DASVII010000050         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_74454, whole
            genome shotgun sequence.
ACCESSION   DASVII010000050 DASVII010000000
VERSION     DASVII010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74454"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..964
                     /locus_tag="VF309_00635"
     CDS             266..964
                     /locus_tag="VF309_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7465114.1"
                     /translation="MRPDDVAASVATDPRLIIPVGTCEQHGRHLPLGSDTIIVEHLSD
                     DLSAEFGILRAPSLEYGVNVQTERGFAGNASLRKKTLHRMLNDLLDTWESTGVKEFIL
                     LTAHEHDPHQEALSTVITREARVRVVDIFAVDFRSLLEGQAEPMHGDEVDTSLMLFLA
                     PDLVRMDLAEDYMMSRDELRRYRRGWLRIPSGSSGSIGRPTLATAAKGAAIYAYIREK
                     VRSRVFLTPEPEGA"
     gene            1068..>1566
                     /locus_tag="VF309_00640"
     CDS             1068..>1566
                     /locus_tag="VF309_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7465115.1"
                     /translation="MRTTVLAVLALAAFAPALRAQNGVVPPMPGATLTLDEAIITARQ
                     NNPIYLSQTNARRTADANIRATRGALLPSADAYFGSRYQEAGQQFVNGAPLSASGNSL
                     QGNYGASLNYSVNAGAFAAAKAATATRDAVDADIIGQSEQLRAAVTQQYLTVLQAQAR
                     SALQDT"
BASE COUNT          278 a          593 c          478 g          217 t
ORIGIN      
        1 ccccacccca agtgccaggg gcatgcggag cgatcgcggc gccagccggg tcgtcgcccc
       61 cgccaggacg gtggcgagga tgcacccaac gagataacct acgcctcgat cggcgagcca
      121 cccgctcgcc atcagcaggg ccgcggcggc ggcggcgggc gccaccagcg cgcgcacgcc
      181 ggcttccgga agcgatcgcc tcagcattcc gtgcagaatg acctaccctt ccccgacggc
      241 gggcccaccg cgccggatca aagagatgcg cccggacgac gtcgccgcga gcgtcgcgac
      301 cgacccgcgg ctcatcatcc cggtggggac ctgtgagcag cacggccgac atcttccgct
      361 cggcagcgac acgatcatcg tcgagcatct ctccgacgat ctctccgcgg agttcggcat
      421 cctgcgcgcg ccaagcctgg agtacggggt gaacgtccaa actgagcgtg gcttcgccgg
      481 caacgcatcg ctgcgcaaga agacgctgca ccgcatgttg aacgatctgc tggacacctg
      541 ggaatccacc ggcgtgaaag agttcatcct gctcacggcg cacgaacacg accctcacca
      601 ggaagcgctc tccaccgtca tcacgcgcga agcacgcgtg cgggtcgtgg acatcttcgc
      661 cgtggacttc cgcagcttgc tcgaaggaca ggccgagccg atgcacggtg acgaggtgga
      721 cacctcgctc atgctctttc tcgcgccgga cctggtgcga atggatctcg cggaagacta
      781 tatgatgtcg cgcgacgagc tccggcgata ccgccgcggc tggctgcgca tccccagcgg
      841 cagctccggc tccatcggcc gcccgaccct cgccaccgcg gccaagggtg cagcgatcta
      901 cgcctatatt cgcgagaagg tccgcagtcg agtctttctc accccggagc ccgaaggcgc
      961 gtgacccgca accggcggac gcgcccggaa tccgattcgt cccatgaaac ctgcggtcca
     1021 ccccccgcgt acgggcgagt gacccaacaa ccaggatagc cctcccgatg cgtacaacgg
     1081 tgctcgccgt gctcgccctc gccgccttcg cccccgctct gcgggcgcag aatggcgtgg
     1141 ttcccccgat gcccggcgcc acgctcacgc tggacgaagc catcatcacc gcccggcaga
     1201 ataacccgat ctatctctca cagacgaacg cccgccgcac cgcggacgcc aacatccgcg
     1261 ccacgcgcgg agcgctgctg ccgtcggccg atgcgtactt cgggagtcgc taccaggaag
     1321 cgggccagca gttcgtgaat ggtgcccccc tgtccgccag cggcaactcc ttgcagggca
     1381 actacggcgc ctcgctgaac tacagcgtca acgcgggcgc cttcgccgcc gccaaggcgg
     1441 ccacggccac gcgcgacgcc gtcgacgccg acatcatcgg ccagtccgag caactgcgcg
     1501 cggcagtgac gcaacagtat ctcaccgtgc ttcaggcgca ggcgcgctct gcgctccagg
     1561 acacgc
//
LOCUS       DASVII010000051         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_541969,
            whole genome shotgun sequence.
ACCESSION   DASVII010000051 DASVII010000000
VERSION     DASVII010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541969"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF309_00645"
     CDS             <1..737
                     /locus_tag="VF309_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7465116.1"
                     /translation="LGAVRLRVYGTYSLRVGDAGTFVRELVGANSSFTIDQITDQVRD
                     LVVSRFSELLGEKAMPILELASNYGELGEMATVKLEPELARLGVELAQLVVESIALPD
                     EVAATLDQRTRIALIGDVSAYAQLQAADAIRDSARNPGGGGAGVGIGVGLAMANKIAG
                     AATQALQPAAPPPMGPPPIPQAAGLFVAENGKPVGPFDRVGLEQRVRGGTLRADTLVW
                     RDGMANWAPASQVTGLEDLLKGHNGV"
     gene            complement(788..967)
                     /locus_tag="VF309_00650"
     CDS             complement(788..967)
                     /locus_tag="VF309_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX7465117.1"
                     /translation="MAERKSFLLRIDRALLEAVQRWANDDLRSVNAQVELLLRDALKR
                     AGRLPKGRSSETDGS"
     gene            complement(981..>1680)
                     /locus_tag="VF309_00655"
     CDS             complement(981..>1680)
                     /locus_tag="VF309_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7465118.1"
                     /translation="GLFIVNPGEAKALQLFGEYKGTVRSQGIWWANPLLTKRSISLRI
                     RNFETAKLKVNDSRANPIEIAAIVVWKVVDTAEALFEVDDYSAYVAVQSESAVRAVAQ
                     AYPYDSHEDNEEALSTHTAEVSQALQTAIHDRLAKAGVEVIEARISHLAYSPEIAQAM
                     LQRQQAGAIIAARSKIVEGAVGMVEHALEMLSRKGIVVLDEERKAQMVSNLLVVLCSE
                     RAASPVVNAGSIFS"
BASE COUNT          254 a          555 c          574 g          297 t
ORIGIN      
        1 agctcggcgc cgtgcgcctg cgcgtgtacg gtacgtactc gctccgcgtt ggcgacgccg
       61 ggacgttcgt gcgcgagctc gtgggtgcga actcgtcgtt taccatcgat cagatcaccg
      121 atcaggttcg cgatctcgtc gtgtcgcgct tcagcgagct gctcggcgag aaggccatgc
      181 cgattttgga gctcgcctcg aactatggtg agctcggcga gatggcgacg gtcaagctgg
      241 agcccgagct ggcgcggctc ggcgtcgagt tggcgcagct cgtcgtggag agcatcgcac
      301 ttcccgacga agttgcggcg acgctcgatc aacgcacacg catcgccctc atcggcgatg
      361 tgagtgccta cgcgcaactg caagctgccg acgcgattcg tgactcggcg cggaatcccg
      421 gcggcggtgg cgctggcgtg ggtatcggcg ttggcctcgc gatggcgaac aagatcgccg
      481 gagcggcgac gcaggcgttg cagcccgccg cgccaccgcc gatgggtccg ccgccgattc
      541 ctcaggctgc cgggctcttt gtggcggaga atggcaagcc cgtcggtccg ttcgatcgcg
      601 tcgggctcga acagcgtgtg cggggcggca cgttgcgcgc ggatacactc gtgtggcgcg
      661 acggcatggc gaactgggct ccagcgtcgc aagtgaccgg gctggaggat ctgctcaaag
      721 gccacaacgg agtgtgagca aacggacgca cggtgtccgc ggagtacgtc gccccccgcg
      781 gttaccgtca gctcccgtcc gtctcggagc tcctgccctt cggcagccgc cctgcgcgct
      841 tgagtgcgtc gcgcaggagc agctcgacct gcgcgttcac ggagcgcagg tcgtcgttgg
      901 cccagcgctg cacggcctcg agcagcgcgc ggtcgattcg gagcaggaag gacttgcgct
      961 cagccacccg gcggccctct ctacgagaag atggagccgg cgttcaccac cggtgacgcg
     1021 gcgcgctccg agcagagcac cacgagcaga ttgctgacca tctgcgcctt gcgctcttcg
     1081 tcgagcacga cgatgccctt gcgcgacagc atctcgagcg cgtgctccac catccccacc
     1141 gcgccttcca cgatcttgga tcgtgcggcg atgatcgcgc ccgcctgctg ccgttggagc
     1201 atggcctgcg cgatctccgg cgagtaggcg aggtgcgaga tgcgcgcttc gatcacctcc
     1261 acgcccgctt tggcgaggcg gtcgtgaatg gccgtctgca acgcctgcga cacctcggcc
     1321 gtgtgtgtgg agagcgcctc ctcgttgtcc tcgtgcgagt cgtacggata cgcctgcgcc
     1381 acggcgcgca ccgcggactc gctctgcacc gcgacatacg cgctgtagtc gtccacctcg
     1441 aacagcgcct cggcggtgtc caccaccttc cacacgacga tggccgcgat ctcgatgggg
     1501 ttggcgcgcg aatcgttcac tttgagcttt gccgtctcga agttgcggat gcgaagcgag
     1561 atggagcgct tggtgaggag ggggttggcc caccagatcc cctgcgagcg cacggtgccc
     1621 ttgtactcgc cgaagagctg gagtgccttg gcctcgccgg gattaacgat gaacagaccg
//
LOCUS       DASVII010000052         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_110647,
            whole genome shotgun sequence.
ACCESSION   DASVII010000052 DASVII010000000
VERSION     DASVII010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110647"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VF309_00660"
     CDS             <1..1705
                     /locus_tag="VF309_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEX7465119.1"
                     /translation="AVVDETRPLAPRASRLRVHWTDRVAHALLVVAGLALALFLLAPM
                     AVILAKSVEDRAGELVGLANFAQYFATPALLRSIWNSVWVSSLVTVLTLPLAFTFAYA
                     LTRSGIRWKTLLRNVALVPILAPSLLAAISFIFWFGNQGVLKPLMGEGQIYGAPGIVG
                     SMVFATFPHALMILVTALSLTDARLYEAADSLGTSAARKFFTITVPGAKYGLISAAMV
                     VFTYAISDFGIPKVIGGNFNMLATDIFKLVIGQQDFAKGAVVAIILLVPVAVTYGVDA
                     YVQKRQSALLSARSVPYVPRRSRRFDWTMAVFCWTLAVLMLAVLGMAIFASFVKLWPY
                     NFSFSLDHYRLGLIDAGIFGAYLNSLHLAAWCATLGAAFIFATAYLLEKTRGLDAWRP
                     FVRLMAVLPMGVPGMVLGLGYIFFFVPEGNPLHFIYGTMSILVLITVVHYYSSSHLTA
                     VTALKQIDNEFEAVSASLRVPFWRTFFRVTVPVCLPAMLDISRYLFVNAMTTVSAVVF
                     LYSPKTMLASVAIVNLDEAGDIGPAAAMASLIVVTSALVCGLYYFAQAWLDKRTQAWR
                     H"
     gene            1752..2879
                     /locus_tag="VF309_00665"
     CDS             1752..2879
                     /locus_tag="VF309_00665"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEX7465120.1"
                     /translation="MIIGNDPILLTPGPLTTSLATKQAMLRDWGSWDAEFNAMTASIC
                     KDLVDVVNGAASHVCVPLQGSGTFAVEAALANLVPRDGKVLVANNGAYCQRMLRILKY
                     LGREHSSIDVAEDKQATAAMIEGALKMDPRITHVAQVHCETGTGILNPLAEIAGVCAR
                     NGKGLIVDAMSSFGAIEIDVAKTPIDAVVAASGKCIEGAPGMGFVIARKALLENCQGN
                     SHSLAMDLHDQWTYMQKTTQWRFTPPTHVVAAFRAALDQFKAEGGTSARGMRYRRNVD
                     TLIDAMSALGFKTFLPKSVQSPIIVTFHAPADPAYSFKAFYEKVRARGYILYPGKLTR
                     VETFRVGCIGAIDANELRNVAAAIGETLKEMGIGRAAAVFV"
     gene            2887..>3490
                     /locus_tag="VF309_00670"
     CDS             2887..>3490
                     /locus_tag="VF309_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7465121.1"
                     /translation="MLGVLFDGIAYGSLLFLVSLGLSVTMGLMNFVNLAHGAFAMAGG
                     YVCVIAMTRFGVPFLAALPLAFVVCAIVGAVLERLLYRRLYGASHLDQVLFSIGLTFM
                     SIATATYFFGSSQQPMRLPEFLRGQVHLGGVDFGIYRLFLVAVVAALTTVLGYLLSRT
                     RFGAQVRASVDNAQAARGLGIDVDRVFSVAFALGSGLAGLG"
BASE COUNT          499 a         1198 c         1119 g          674 t
ORIGIN      
        1 agccgtggtg gatgagacgc ggcctcttgc gcctcgtgcc tcgcgcctgc gagtccattg
       61 gaccgaccgc gtcgcccacg cgctcctcgt cgtggccggg ctcgcgctcg cgctcttcct
      121 cctcgcgccg atggccgtca tcctcgcgaa gagcgtggag gaccgcgccg gcgagttagt
      181 cggcctcgcg aatttcgcgc agtacttcgc cacgcccgcg ttgttgcgct ccatctggaa
      241 cagcgtctgg gtgtcgagcc tggtgacggt gctcacgctg ccgctcgcgt tcaccttcgc
      301 ctacgcgctc acgcgcagcg gcatccgctg gaagacgctg ctacgcaacg tcgcgttggt
      361 gccgatcctc gccccgtcgc tcctcgcggc gatctcgttc atcttctggt tcggcaacca
      421 gggcgtgctg aagccgctca tgggcgaggg ccagatctac ggcgcgccgg ggatcgtggg
      481 gtcgatggtc tttgccacgt ttccccacgc gctcatgatc ctcgtgaccg cgctctcgct
      541 caccgacgcg cggctctacg aggcggccga ttcgctcggc accagcgccg cgcgcaagtt
      601 cttcacgatc accgtgccgg gcgccaagta cggcctcatc agcgccgcga tggtggtgtt
      661 cacctacgcc atcagcgact tcggcattcc caaggtgatc ggcggcaact tcaacatgct
      721 cgcgaccgac atcttcaagc tggtgatcgg gcagcaggac ttcgccaagg gcgcggtggt
      781 ggccatcatc ctgctcgtgc ccgtggcggt gacctacggg gtggacgcat acgtgcagaa
      841 gcgccagtcg gcgctgctct cggcgcgctc cgtgccttac gtgccgcggc gctcgcgccg
      901 cttcgactgg acgatggcgg tgttctgctg gacgcttgct gttcttatgc tcgcggtgct
      961 cggcatggcg atcttcgctt ccttcgtgaa gctctggccc tacaacttca gcttttccct
     1021 cgaccactat cgattggggc tcatcgacgc gggaatcttc ggcgcctacc tcaacagcct
     1081 ccatctcgcc gcctggtgcg cgaccctcgg cgctgccttc atcttcgcga cggcctacct
     1141 tctcgagaag acgcggggcc tcgacgcgtg gcgccccttc gtgcggctga tggcggtgct
     1201 gcccatgggc gttcccggca tggtgctggg gctgggctac atcttcttct tcgtgcccga
     1261 gggcaatccg ctgcatttca tctacggcac gatgtcgatc ctcgtgctga tcaccgtggt
     1321 ccactattat tcgtcctcgc atctcacggc ggtgaccgcg ttgaagcaga tcgacaacga
     1381 gttcgaggcg gtatcggcat cgcttcgcgt gccgttctgg cgcacattct tccgcgtgac
     1441 ggtgccggtg tgcctccccg cgatgctcga catcagccgc tatctcttcg tgaacgcgat
     1501 gaccaccgtc tcggcggtcg tgttcctcta ctcgccgaag accatgctcg cctccgtcgc
     1561 catcgtgaac ttggacgagg cgggcgacat cgggcccgcc gcggcgatgg cgagcctgat
     1621 cgtggtcacg tccgcgctgg tgtgcgggct ctactatttc gcgcaggcct ggctcgacaa
     1681 gcggacccag gcgtggcgtc attagccgtc atcccggcca accccgggac tcaatgcacc
     1741 aggagatgcc catgatcatc ggcaacgatc ccatcctcct cacccccggc ccgctcacca
     1801 cgtcgctcgc caccaagcag gcgatgctgc gcgactgggg ctcgtgggac gcggagttca
     1861 atgcgatgac ggcttcgatc tgcaaggacc tcgtcgacgt ggtgaacggc gcggcgagcc
     1921 acgtgtgcgt tccgctgcag gggagcggca cgttcgcggt ggaggcggcg ctcgccaacc
     1981 tcgtgccgcg cgacggcaag gtgctggtgg ccaataacgg cgcctactgc cagcgcatgc
     2041 tgcgcatcct caagtacctc gggcgcgagc actcgtcgat cgacgtcgcc gaggacaagc
     2101 aggcgacagc ggcgatgatc gagggcgcgc tgaagatgga cccgcgcatc acccacgtgg
     2161 cgcaggtgca ttgcgagacc ggaacgggaa tcctcaatcc gctggcggag atcgccgggg
     2221 tgtgcgcgcg caacggcaag gggctcatcg tcgatgccat gagctcgttc ggcgcgatcg
     2281 agatcgacgt cgccaagact cccatcgatg cggtggtcgc cgcatccggg aaatgcatcg
     2341 agggcgcgcc gggaatggga ttcgtcatcg cgcgcaaggc gctcctcgag aattgccagg
     2401 gcaactcgca ctcgctcgcc atggacctgc acgaccagtg gacctacatg cagaagacga
     2461 cgcaatggcg cttcacgccg cccacgcacg tggtcgcggc ctttcgcgcg gcgctcgacc
     2521 agttcaaggc cgagggcgga acgagcgctc gcggaatgcg ctatcgccgc aacgtggaca
     2581 cgctgatcga tgcgatgtcg gccctcgggt tcaagacctt cctcccgaag agcgtgcagt
     2641 cgcccatcat cgtgaccttc cacgcgcccg cggacccggc gtattcgttc aaggcgttct
     2701 acgagaaagt gcgcgcgcgc ggctacatcc tgtatcccgg caagctcacg cgggtcgaga
     2761 ccttccgcgt cggctgcatc ggggcgatcg acgccaacga gctgcgcaac gtcgcagccg
     2821 ccatcggcga gacgctgaag gagatgggga tcgggagagc ggcagcagtt tttgtatgat
     2881 ggggtgatgc ttggcgtcct cttcgacggt atcgcctacg ggagcctcct cttcctggtg
     2941 agcctgggcc tctcggtcac gatgggcctc atgaacttcg tgaacctcgc gcacggcgcg
     3001 ttcgcgatgg cgggcggcta cgtgtgcgtg atcgcgatga cgcgctttgg agtgcccttc
     3061 ctcgcggcgt tgcccctcgc cttcgtcgtc tgcgcgatcg tcggcgcggt gttggagcgc
     3121 cttctctacc gccgcctcta tggcgcgagc cacctcgacc aggtcctctt ctcgatcggc
     3181 ctcaccttca tgtcgatcgc gaccgcgacg tacttcttcg gctcctcgca gcagccgatg
     3241 cggcttccgg aattccttcg cgggcaggtg cacctcggcg gcgtggactt cggcatctac
     3301 cggctcttcc tcgtcgcggt cgtcgcggcc ctcaccacgg tgctcggata cctcctctcg
     3361 cgcacgcgct tcggcgccca ggtgcgcgct tcggtggaca acgcgcaggc cgcgcgcggg
     3421 ctcggcatcg acgtggaccg cgtcttcagc gtcgccttcg cgctgggctc ggggctcgcg
     3481 ggcctgggcg
//
LOCUS       DASVII010000053         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_182444,
            whole genome shotgun sequence.
ACCESSION   DASVII010000053 DASVII010000000
VERSION     DASVII010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182444"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="VF309_00675"
     CDS             <1..1651
                     /locus_tag="VF309_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX7465122.1"
                     /translation="RFAGQRALLLDAVAMGLLRKYLVENFGLTAARTVLTQFGFAHGW
                     RMAEAMQAEFKWESAEDWCRAGTRIHALEGLFRVDAGNKDALSKEGVMLDASYEAEQH
                     LLHFGRSDEPACWTISGLTSGYISRSSGKEIYVLEDRCLGKGDVACHLLGRTREEWGD
                     ERAEELRFFEPIRLKESLDVSLHRVTETLKAAERKLRERRRALVRVVPDVDEPLGIIA
                     KSPAMRKIVDLARRVAKVDSTVLITGESGSGKERIARLVHDESTRVAGPFIAVNCGAI
                     TETLLESELFGHARGAFTGATQDRPGLFEAANNGTLLLDEVGEVSPGMQVKLLRALQE
                     REIRRVGENKSRRIDVRVVAATNRDLALRVASGDFRQDLYYRLKVVELHVPPLRERRD
                     DALPLARVLLAEAALRMKRKIAGLAPGAADQLLRYEWPGNVRELENAMERAVALARGS
                     RVELEDLPEEIRQAFARPVATQGAVRPLDDIERDYILAALEVNAGNQTHTAAQLHIGS
                     ATLYRKLKSYGKIGNKHGSKAGGGALPDTSVLTSSANASST"
     gene            1685..>2073
                     /locus_tag="VF309_00680"
     CDS             1685..>2073
                     /locus_tag="VF309_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465123.1"
                     /translation="MVRSLIRSAMLFTTIVLAGAIARAGTPSPAETEGNKMEKKGNAE
                     EKAANAEKAKGARMEKKGKAIEEAGDKNDNPGQEAAGKKMKKKGHATEKEGEARGDAA
                     EQMEKSGNKTEKAGADARKDAAKAKADA"
BASE COUNT          386 a          687 c          731 g          269 t
ORIGIN      
        1 ccgctttgcg gggcagcgcg cgctgcttct cgatgcggtg gcgatgggcc tcttgcgcaa
       61 gtacctcgtc gagaacttcg gcctcacggc cgcgcgcacc gtgctcaccc agttcggatt
      121 cgcccacggc tggcgcatgg ccgaggcgat gcaggccgag ttcaagtggg agagcgccga
      181 ggactggtgc cgcgccggca cccgcatcca cgccctcgaa gggctcttcc gcgtcgacgc
      241 cggaaacaag gatgccctgt ccaaggaggg ggtgatgctc gatgcctcct acgaggccga
      301 gcagcacctg ctgcacttcg gtcgctcgga cgagccggcg tgctggacca tctccggcct
      361 gaccagcggc tacatcagcc ggagctcggg caaggagatc tacgtcctgg aagaccgctg
      421 tctgggcaag ggggacgtcg cctgccatct cctgggacga acgcgcgaag agtggggcga
      481 cgagcgcgcc gaggagctgc gcttcttcga gccgattcgc ctcaaggagt cgctcgacgt
      541 ctcgctccat cgggtcaccg agaccttgaa ggcggccgag cggaagctcc gcgagcgccg
      601 tcgagcgctg gtgcgcgtgg tgcccgacgt ggatgagccc ctgggaatca tcgccaagag
      661 ccccgccatg cggaagatcg tcgatctggc ccgtcgtgtc gccaaggtcg attccaccgt
      721 cctcatcacg ggggagagcg gctcgggcaa ggagcggatc gcgcgcctgg tccacgacga
      781 atcgacgcgc gtggcggggc cattcatcgc cgtgaactgc ggcgcgatca ccgagaccct
      841 gctcgagagc gagctcttcg ggcacgcgcg cggcgccttt accggcgcca cccaggaccg
      901 gccaggcctg ttcgaggcgg ccaacaacgg gaccttgctg ctcgacgagg tcggcgaggt
      961 ctcgcccggc atgcaggtca agctgctgcg cgcgctgcag gagcgggaga tccgccgcgt
     1021 cggcgagaac aagagccgac gcatcgacgt gcgcgtcgtg gcggcgacca accgcgacct
     1081 cgcccttcgc gtcgcgagcg gcgacttccg gcaagacctg tactaccggc tgaaggtcgt
     1141 cgagctgcac gtgccgcctc tgcgcgagcg ccgagacgac gccctgccgc tggcccgggt
     1201 gctgctggcg gaggcggcgc tgcggatgaa gcgcaagatc gccggcctcg ccccgggcgc
     1261 cgctgatcag ctgctccgct atgaatggcc gggcaacgtg cgcgagctgg agaacgcgat
     1321 ggagcgcgcc gtcgcgctgg cgcgcgggag ccgggtcgag ctcgaggatc tcccggagga
     1381 gatccggcag gcctttgcca ggccggtcgc cacccagggc gcggtgcgtc cgctcgatga
     1441 catcgagcgg gactacatcc tcgcggcgct cgaggtcaac gcgggcaacc agacccacac
     1501 cgcggcgcag ctccacatcg gctcggcgac gctgtaccgc aagctgaaga gctacggcaa
     1561 gatcgggaac aagcacggca gcaaggccgg aggcggcgcc cttcccgata cgtcggtttt
     1621 gacaagctct gcgaatgcat cttcgacata ggcgaagttc acatccacaa ccgaggaggc
     1681 tccaatggtc cgttccctga tccgctccgc aatgctcttc accaccatcg ttcttgcagg
     1741 cgccatcgcg cgggccggca cgcccagccc cgccgagacg gagggcaaca agatggagaa
     1801 gaagggaaac gccgaggaga aggccgccaa cgccgagaag gccaagggcg cccgcatgga
     1861 aaagaagggc aaggccatcg aggaggccgg cgacaagaat gacaatccgg gccaggaggc
     1921 ggccggcaag aagatgaaga agaaggggca cgccaccgag aaagagggcg aggcccgtgg
     1981 cgatgccgcc gagcagatgg agaagtcggg caacaagacc gaaaaggccg gcgccgacgc
     2041 gcgcaaggat gccgccaagg ccaaggccga cgc
//
LOCUS       DASVII010000054         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_146738,
            whole genome shotgun sequence.
ACCESSION   DASVII010000054 DASVII010000000
VERSION     DASVII010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146738"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1761
                     /gene="uvrC"
                     /locus_tag="VF309_00685"
     CDS             <1..>1761
                     /gene="uvrC"
                     /locus_tag="VF309_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7465124.1"
                     /translation="AAEFVSGLPGLPGVYRFLNADGVAIYVGKAGDLRKRVASYFQKR
                     DQSPRTAMMISQIAGAETTVTRTEAEALILENNLIKSLAPRYNVLFRDDKSYGYILVT
                     GHKFPQIRFYRGAHQKGNKYFGPFPSAWSIRETIGHLQKIFKLRTCDDTVFNHRSRPC
                     LLHQIGRCSGPCVGLVTEDQYAADVRHAEQFLEGKESDVIEDLTSRMTLAAEDRHYER
                     AAEYRDQVRMLQRILSGQAVESTGKGDVDVIVAVERDNVWCVTLAMVRGGRHLGDRSF
                     FPSNASGTDARTVVESFVDQHYAQQPVPGRVIADQVEHAPETEAVLGAIAGRAVKLVT
                     RPIGENRTWTEMARRNASLALGQRLALQATQEARVIALQEFLGIETPIQRIECFDISH
                     TMGEATVGSCVVYDKGAMQNSEYRRYNVKGVDPGDDYGAMCYALGVRYRKLAQGEGKT
                     PDLILIDGGLGQINAAKAVMQDLGLHHIRMIGVAKGVERKPGMEQLFVAGEDEPRRLP
                     PDHPALHLIQQIRDEAHRFAITGHRARRGKARTASRLEEISAVGPRRRQRLLEHFGGL
                     QGVLAASIEDLARVEGISRT"
BASE COUNT          312 a          615 c          577 g          257 t
ORIGIN      
        1 atgccgccga gtttgtaagc ggccttccag gccttcccgg cgtctaccgc ttcctcaacg
       61 cggatggcgt cgcgatctac gtcggcaagg cgggcgacct tcgcaagcgc gtcgcgtcgt
      121 acttccagaa gcgcgaccag agcccgcgca ccgcgatgat gatctcgcag atcgcggggg
      181 cggagaccac ggtcacgcgc accgaggccg aggcgctgat cctcgagaac aacctcatca
      241 agagcctcgc gccgcgctac aacgtgcttt tccgcgacga caagagctac ggctacatcc
      301 tcgtcaccgg ccacaagttc ccgcagatcc gcttctaccg cggcgcgcac cagaagggca
      361 acaagtactt cgggccgttt cccagcgcgt ggtcgatccg cgagaccatc gggcacctgc
      421 agaagatctt caagctgaga acctgcgacg acacggtctt caaccatcgc tcgcgcccct
      481 gcctgctgca ccagatcggc cgctgctccg gaccctgcgt ggggctcgtc accgaggatc
      541 aatacgccgc cgacgtgcgc cacgccgagc aattcctcga gggcaaggag agcgacgtga
      601 tcgaggacct cacctcgagg atgacgctcg ccgccgagga tcgccattac gagcgcgccg
      661 cggaataccg cgaccaggtc cgcatgttgc agaggatcct ctcgggccag gcggtggaat
      721 cgacggggaa gggcgacgtc gacgtgatcg tcgccgtcga gcgcgacaac gtgtggtgcg
      781 tgacgctcgc gatggtgcgc ggcgggcggc atctcggcga tcgcagcttc tttccgtcga
      841 acgcttccgg caccgacgcg cgcaccgtcg tcgaatcctt cgtcgaccag cactacgcgc
      901 agcagcccgt gcccgggcgc gtgatcgccg accaggtcga gcatgcgccg gagaccgaag
      961 cggtgctggg cgccatcgcc ggtcgcgcgg tgaagctcgt gacccggccc atcggcgaga
     1021 accgcacctg gaccgagatg gcgcgccgca acgcgtccct cgcgctgggc cagcgcctcg
     1081 cgctgcaggc cacgcaggaa gcgcgcgtca ttgcgctgca ggaattcctc ggcatcgaga
     1141 cgccgatcca gcgaatcgag tgcttcgaca tcagccacac gatgggggag gcgaccgtgg
     1201 gctcctgcgt cgtctacgat aagggcgcga tgcagaattc cgaataccgc cgctacaacg
     1261 tgaagggcgt cgatcccggc gacgactatg gcgccatgtg ctacgcgctc ggcgtgcgct
     1321 atcgcaagct cgcgcagggc gaaggcaaga cgccggactt gatcctcatc gacggcggcc
     1381 tcgggcagat caacgccgcg aaggcggtga tgcaggatct cggcctgcac cacatccgca
     1441 tgatcggcgt ggccaaggga gtcgagcgca agcccggcat ggagcagctc ttcgtcgccg
     1501 gggaggacga gccgcggcgc ctcccgcccg atcaccccgc gctgcacctc atccagcaga
     1561 tccgcgacga ggcccatcgc ttcgccatca ccggccaccg cgcgcgccgc ggcaaggcgc
     1621 gcaccgcctc gcggctcgag gagatttccg ccgtggggcc gcgccgccgc cagaggctcc
     1681 tcgagcactt cggtggactt cagggtgtat tggctgcgag tatcgaggat ctggcgcgcg
     1741 tggaaggcat cagccgcacg c
//
LOCUS       DASVII010000055         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_326552,
            whole genome shotgun sequence.
ACCESSION   DASVII010000055 DASVII010000000
VERSION     DASVII010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326552"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VF309_00690"
     CDS             <1..1117
                     /locus_tag="VF309_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7465125.1"
                     /translation="CVQVRRAKLIVAVALAILAAGLYQAHHLKVGDYGIGVPELRPDS
                     RYNLDNAKIVDKFAIGVNMLGVVAQTHNVQGACTQYEVMSLIERFDWYMQNVPGTQSV
                     ISLPGLAKVVNAGYNEGNIKWRQLPRDPQVMAQAVTPIDTSTGLLNPDCSAMQVLVNT
                     TDQQGETIARLVSEVKRFSAANPSDKVELKLATGNVGVMAATNEAVDQARWEMHFAIF
                     GALAVMCMVTFRSLRATLCILIPLAIVSVLCEALMPLLGIGLKVSTLPVIALGVGVGV
                     DYGIYLYDRIEKHLEEGKDLAVSFYEALNERGTAMVFTAVTMTIGVGTWAFSALKFQA
                     DMGLLLAFMFFLNMLGALFLLPALAAFLLKRPSAPKP"
     gene            1120..1491
                     /locus_tag="VF309_00695"
     CDS             1120..1491
                     /locus_tag="VF309_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX7465126.1"
                     /translation="MSRFFLVCGALVMASAVALGAYAAHAAKGAAHPEAARLLQTAVQ
                     YQLVHGLGILAVGLAARNGASRWLAASGLLLIAGVILFCGSLWVLALANQSLGPAAPL
                     GGLAFIAGWLALAVHALRMRT"
     gene            complement(1534..>2043)
                     /locus_tag="VF309_00700"
     CDS             complement(1534..>2043)
                     /locus_tag="VF309_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465127.1"
                     /translation="VSELAGGALRAITVRNGAITGFAQAVFFPKSIAVVVERITATDN
                     TGNAITAGDMATVRDSATHVNGGNGISLGLFSLVRGNASSENGGLGIFVSTGGHIVDN
                     DVAHNGGVGINAGEGANVVHNVSRNNGTNGIFVECPSAVVGNAATNNLSQNIVLLGGA
                     CVSDHNSDL"
BASE COUNT          298 a          691 c          680 g          374 t
ORIGIN      
        1 ttgcgtccag gtgcggcgcg cgaagctcat cgtcgcggtc gccctggcga tcctcgccgc
       61 gggcctctac caggcgcacc acctcaaggt cggcgactat ggcatcggcg tgccggaact
      121 gcgcccggat tcgcgctaca accttgacaa cgcgaagatc gtcgacaagt tcgcgatcgg
      181 cgtgaacatg ctcggcgtgg tcgcgcagac ccataacgtg cagggcgcct gcacccagta
      241 cgaggtgatg agcttgatcg agcgcttcga ctggtacatg cagaacgtgc ccggcacgca
      301 gtcggtgatc tcgctgccgg ggctcgccaa ggtcgtgaac gccggctaca acgagggcaa
      361 catcaagtgg cggcagctac cgcgcgaccc gcaggtgatg gcgcaggcgg tgacgccgat
      421 cgacacgtcc acgggattgc tcaaccccga ttgcagcgcg atgcaggttc tcgtcaacac
      481 caccgaccag caaggcgaga ccatcgcccg cctggtgagc gaggtgaagc gcttctccgc
      541 cgcgaacccg agcgacaagg tcgagctgaa gctcgccacc ggcaacgtgg gcgtgatggc
      601 cgcgaccaac gaggcggtcg accaggcacg ctgggaaatg catttcgcca ttttcggcgc
      661 gctcgccgtg atgtgcatgg tcacgttccg cagcctgcgc gcgacgctct gcatcctcat
      721 cccgctcgcc atcgtctcgg tgctgtgcga ggcgttgatg ccgttgctcg gcatcggcct
      781 gaaggtctcg acgctcccgg tgatcgcgct cggcgtcggc gtgggcgtcg actacgggat
      841 ctatctctac gaccgcatcg agaagcacct cgaggaaggc aaggacctcg cggtctcgtt
      901 ctacgaggcg ctgaacgagc gcggcacggc gatggttttc accgcggtca ccatgacgat
      961 cggcgtgggc acgtgggcct tctcggcgct caagttccag gcggacatgg gactccttct
     1021 cgccttcatg ttcttcctga acatgctcgg cgcgctcttt ctcctgccgg cgctcgcggc
     1081 gttcctgctg aagcgtccct ccgcgccgaa gccgtaggca tgtcgcgctt cttcctcgtc
     1141 tgcggcgcgc tcgtcatggc ttcggccgtc gcgctggggg cgtatgccgc ccacgcggcg
     1201 aagggcgcgg cgcaccccga ggccgcgcgg ctgctgcaaa ccgcggtgca gtaccagctg
     1261 gtccacggcc tcggcatcct cgccgtcggg ctcgcggcgc gcaacggcgc gtcgcggtgg
     1321 ctcgcggcgt cgggcctcct cctcatcgcg ggcgtgatcc tcttctgcgg ctcgctgtgg
     1381 gtgctggcgc tggcgaatca atcgctcggg cccgccgcgc cactcggcgg cctcgccttc
     1441 atcgccggct ggctcgcgct cgccgtccac gccctccgaa tgcgtacctg acacgaattt
     1501 ctagtgcggc aggcatttcg tcgatggggc ggatcaaagg tcggagttgt ggtccgatac
     1561 gcaggcgccg ccgagaagga cgatgttctg gctgaggttg ttggtcgcgg cgttgcctac
     1621 gaccgcgctc gggcattcga cgaagattcc gttcgtgccg ttgttgcgcg agacgttgtg
     1681 gaccacgttc gcgccctcgc cggcgttgat cccgaccccg ccgttgtgcg ccacgtcgtt
     1741 gtcgacgatg tggccgccgg tgctcacgaa gattccgagg ccgccgttct cgctggatgc
     1801 attgcccctc acgagcgaga aaagccccag cgagatgccg ttgccgccgt tcacgtgcgt
     1861 tgcgctgtct cgcaccgtcg ccatgtcgcc ggcggtgatc gcgttaccgg tgttgtcggt
     1921 tgcggtgatc cgctcgacga ccacggcgat ggacttcggg aagaagaccg cctgcgcgaa
     1981 gccggtgatg gcgccgttgc gaaccgtgat tgcgcgcagc gcgccgcccg ccagctccga
     2041 gac
//
LOCUS       DASVII010000056         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_110795,
            whole genome shotgun sequence.
ACCESSION   DASVII010000056 DASVII010000000
VERSION     DASVII010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110795"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VF309_00705"
     CDS             <1..737
                     /locus_tag="VF309_00705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7465128.1"
                     /translation="EITDYISSIGARLLSVADPPHRDIDFFVVQDDTINAFALVGGHI
                     GVHSALIMLTQSESELAGVMAHEIAHILQRHQARMIHGASRAQWTSLAALALAILAAR
                     GGGAQAGQVTEAAVATAGALQIQNQIDYTREHEREADRVGLTLLERAGFDTRGMATFF
                     ERMLRSNRLNEFKGAPSYLRTHPLTTERIADMQGRVEGTPTRMVPDSFDYRIARSKVR
                     AAMARSCRSSAWRRSRTPPSRSSPAS"
     gene            689..1162
                     /locus_tag="VF309_00710"
     CDS             689..1162
                     /locus_tag="VF309_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465129.1"
                     /translation="MAPQPHPAFEVLAGQLMADMGKHDEAATIYRRALRGNPHYRSLV
                     YAYLDELLQTGHAKEGLADLEERLRTTQDDYRLYELQARAYESLGRPIAQHRAQGEAY
                     FRKGNLAAAVDQLEIAVKQTRGSNFYEMSIAESRLRELRVLLENERAAEKALKIT"
     gene            1162..1500
                     /gene="soxX"
                     /locus_tag="VF309_00715"
     CDS             1162..1500
                     /gene="soxX"
                     /locus_tag="VF309_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX7465130.1"
                     /translation="MRKTAAVLALACGIATSASAQVPARDLFVRADKGHCIACHQLPE
                     GAGPQTRADLGPRLEGARMRELGKPALREAIVDPTRANPDTVMPPFGRHHILDAAEIE
                     RLVEFLHALP"
BASE COUNT          259 a          611 c          523 g          215 t
ORIGIN      
        1 cggagatcac cgactacatt tcctcgatag gcgcgcgcct gctctcggtg gccgatcctc
       61 cgcaccggga catcgacttc ttcgtggtgc aggacgacac catcaacgcc ttcgcgctcg
      121 tgggcgggca catcggcgtg cactccgcgc tcatcatgct cacgcagagc gaatcggagc
      181 tcgccggcgt gatggcccac gagatcgcgc acatcctgca acgccaccag gcgcgcatga
      241 tccacggcgc gagccgtgcg cagtggacct cgctcgcggc gctcgccctc gccatcctcg
      301 cagcgcgcgg cggcggtgcg caggcgggcc aggtcaccga ggcggcggtc gccaccgcgg
      361 gcgcgctgca gatccagaac cagatcgact acacccgcga gcacgagcgg gaagccgacc
      421 gcgtaggtct caccctcctc gagcgagccg gcttcgacac gcgcgggatg gcgaccttct
      481 tcgagcggat gctccgctcc aaccgcctga acgagttcaa gggcgcgcct tcctacctgc
      541 gcacgcaccc gctcaccacg gagcgtatcg ccgacatgca agggcgcgtc gagggcacgc
      601 cgacccggat ggtcccggat tcgttcgact accgcatcgc gcgctcgaag gtccgcgcgg
      661 cgatggcaag gagctgccgg tcttcggcat ggcgccgcag ccgcaccccg ccttcgaggt
      721 cctcgccggc cagttgatgg cggacatggg caaacacgac gaggccgcca cgatctaccg
      781 ccgggcgctg cgcggcaacc cgcactaccg ctcgctggtc tacgcctacc tcgacgagct
      841 gctgcagacc ggccacgcca aggagggcct ggccgacctc gaggagcgcc tgcgcacgac
      901 gcaggacgac taccgcctct acgagctgca agcgcgcgcg tacgagtcgc tcgggcggcc
      961 catcgcgcag caccgcgccc agggcgaggc gtatttccgc aagggcaacc tcgccgccgc
     1021 ggtggaccaa ctggagatcg cggtgaagca gacgcgcggc agcaacttct acgagatgtc
     1081 catcgccgag tcgcggctgc gcgagctgcg cgtcctcctc gagaacgaac gcgccgccga
     1141 gaaggcgctc aagatcacct gatgcgaaag accgccgcgg tcctcgccct cgcctgcggg
     1201 atcgccacga gcgcatcggc gcaagtcccc gcccgcgatc tcttcgtgcg cgccgacaag
     1261 ggccattgca tcgcctgcca ccagcttccg gagggcgcgg gtccgcaaac gcgcgccgac
     1321 ctgggcccgc gcctggaggg tgcgcgcatg cgcgagctgg gcaagcccgc gctacgcgag
     1381 gcgatcgtcg atcccacgcg cgccaacccc gacaccgtga tgccgccttt cggccgccac
     1441 cacatcctcg acgccgcgga gatcgagcgg ctggtggaat tcctccatgc gcttccctga
     1501 cgcgcgcctc ctcgccgcgg tcgccgcgtt cggggtcgcg catgccttcg ccgcggccga
     1561 cgcggtgaag accgagatcg tcgcgcgcct tcagaagcag ctccccgg
//
LOCUS       DASVII010000057         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_110903,
            whole genome shotgun sequence.
ACCESSION   DASVII010000057 DASVII010000000
VERSION     DASVII010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110903"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VF309_00720"
     CDS             complement(<1..729)
                     /locus_tag="VF309_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7465131.1"
                     /translation="MGIALSQERDIHKLLETILIAAKNLTHADGGTLYRLDGDKLQFE
                     IVRTDSLSIAMGGTSGNPVPFYPIPLHDKNGDPNYTMIAAYAALMQRTVNIADAYLEE
                     GFDFSGTRNFDKRTGYRSTSFLTVPMKNHEGDIIGVLQLLNAIDPATGIVTSFSEEDQ
                     CLAESLASQAAIALTNRLLIQQLEVLFESLIELINTAIDDKSPYTGGHCKRVPTLTMM
                     LAEAAASATLGPLADFRMSEKDRYE"
     gene            complement(867..>2210)
                     /locus_tag="VF309_00725"
     CDS             complement(867..>2210)
                     /locus_tag="VF309_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX7465132.1"
                     /translation="GSESIGDLLGGLDTEEDMDPGADDVSAAADNELVKLVNKIIVDA
                     YHQGASDIHVEPLPGKGKTGVRFRKDGTLSNYIEVPAAYRQPLVTRLKIMCDLDISEK
                     RRPQDGKIKFKKFGPLDIELRVATIPTAGGVEDVVMRILAAGEPIPLDKLGVLPQNME
                     RLKGCISKPYGLFFVCGPTGSGKTTTLHSVLGSLNTVDTKIWTAEDPVEITQKGLRQC
                     QMNPKAGLTFAVAMKAFLRADPDIIMVGEMRDKDTTSIGIEASLTGHLVFATLHTNSA
                     PESIIRLLDMGMDPFNFADAILGILAQRLAKRLCKCKEAYTPSQEEVKHFITEYCGEL
                     VNTPEFKTDLQGSYKKVFERFAKDYGFGKPEFKLMKAKGCDACGGTGYKGRVGLHELL
                     VGTDPIKKLIQEHARVAEMLAVALAEGMLTLKMDGMEKVLQGVTDMAQVRAVCIK"
BASE COUNT          313 a          702 c          759 g          436 t
ORIGIN      
        1 ctcgtagcgg tccttttccg acatgcggaa atccgcgagc ggcccgaggg tggcgctcgc
       61 cgcggcctcc gccagcatca tcgtgagcgt cggcacgcgc ttgcagtgcc cgccggtgta
      121 ggggctcttg tcgtcgatcg cggtgttgat gagctcgatc agcgactcga agagcacctc
      181 gagctgctgg atgaggagcc ggttggtgag cgcgatcgcc gcctgggagg cgagggattc
      241 ggcgaggcac tggtcctcct cgctgaagct cgtcacgatg cccgtggccg gatcgatcgc
      301 gttcagcagc tgcagcacgc cgataatgtc gccctcgtgg ttcttcatcg gcaccgtgag
      361 gaacgaggtc gagcggtagc ccgtgcgctt gtcgaagttg cgcgtgccgg agaagtcgaa
      421 gccctcctcg aggtaggcgt cggcgatgtt cacggtgcgc tgcatgagcg ccgcgtacgc
      481 cgcgatcatc gtgtagttgg gatcgccgtt cttgtcgtgc agcgggatgg gatagaacgg
      541 gaccgggttg ccggaggtcc cgcccatcgc gatcgagagc gaatcggtgc gcacgatctc
      601 gaactggagc ttgtcgccgt cgaggcggta gagcgttccg ccgtcggcgt gcgtgaggtt
      661 cttcgccgcg atgaggatcg tctcgaggag cttgtggatg tcgcgctcct gcgagagcgc
      721 gatgccgatc gcgttgaggt actcgagctt ggagaggaag tgcgtctcga gcgcgcgcgc
      781 gagctccgcg ggcgccctcg cgacggcggg ctggttcccc gacgcctcgg gcacggccgt
      841 gcttggtttc gtcgcgttgc gcacggtcac ttgatgcaga ccgcccgtac ctgcgccatg
      901 tcggttacgc cctggaggac cttctccatg ccgtccatct tcagtgtgag catcccttcg
      961 gccagcgcga ccgcgagcat ctcggccacg cgcgcgtgct cctggatgag cttcttgatg
     1021 ggatcggtgc ccaccagcag ctcgtgcagg cccacccggc ccttgtagcc ggtgccgccg
     1081 cacgcgtcgc atcccttggc cttcatcagc ttgaattcgg gcttgccgaa gccgtagtcc
     1141 ttggcgaagc gctcgaacac cttcttgtag ctgccctgca ggtcggtctt gaactcgggc
     1201 gtgttcacga gctcgccgca gtactcggtg atgaagtgct tcacctcctc ctggctcggc
     1261 gtgtacgcct ccttgcactt gcacaggcgc ttggcgaggc gctgcgcgag gatgccgagg
     1321 atcgcgtcgg cgaagttgaa cgggtccatc cccatgtcga ggaggcggat gatcgattcc
     1381 ggcgcggagt tcgtgtgcag cgtcgcgaac acgaggtggc ccgtgagcga ggcctcgatg
     1441 ccgatcgagg tggtgtcctt gtcgcgcatc tcaccgacca tgatgatgtc cggatcggct
     1501 cgcaggaagg ccttcatcgc gacggcgaag gtgagccccg ccttcgggtt catctggcac
     1561 tggcgcaggc ccttctgcgt gatctcgacc gggtcctccg cggtccagat cttggtgtcg
     1621 acggtgttca acgagccgag caccgagtgc agcgtcgtgg tctttccgga gcccgtcggg
     1681 ccgcacacga agaagagtcc gtaaggcttc gagatgcagc ccttcaggcg ctccatgttc
     1741 tgcggcagca cgcccagctt gtcgagcggg atcggctcgc cggccgcgag gatgcgcatc
     1801 accacgtcct cgacgccgcc cgcggtgggg atcgtcgcca cgcgcagctc gatgtcgagc
     1861 gggccgaact tcttgaactt gatcttgccg tcctgcgggc ggcgcttctc cgagatgtcg
     1921 aggtcgcaca tgatcttcag gcgcgtgacc agcggctggc ggtacgccgc gggcacctcg
     1981 atgtagttgg agagcgtgcc gtccttgcgg aagcgcacgc ccgtcttgcc cttgccgggg
     2041 agcggctcca cgtggatgtc cgacgcgccc tggtggtagg cgtcgacgat gatcttgttc
     2101 acgagcttca ccagctcgtt gtcggccgcc gcggagacgt cgtccgcgcc gggatccatg
     2161 tcctcctcgg tatcgagccc gccgaggagg tccccgatcg actcgctgcc
//
LOCUS       DASVII010000058         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_3082, whole
            genome shotgun sequence.
ACCESSION   DASVII010000058 DASVII010000000
VERSION     DASVII010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..477)
                     /locus_tag="VF309_00730"
     CDS             complement(175..477)
                     /locus_tag="VF309_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7465133.1"
                     /translation="MPNSLENVPVNGHEADPALVREIAGLVVSALNLEVKADEIDPAA
                     PLYGEGLGLDSIDILEVALVVSKRYGFKLKEDDQDNLRIFRSLNNLAAHVAERRTK"
     gene            complement(539..1462)
                     /gene="prmB"
                     /locus_tag="VF309_00735"
     CDS             complement(539..1462)
                     /gene="prmB"
                     /locus_tag="VF309_00735"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7465134.1"
                     /translation="MAGARTVRALVAHAIDEFEKAGLSYGHGTANALDEAAWLILHAL
                     GLPPVNLDAHLDTPLTAAQVKAATALIDARVRTRKPAAYLMHEAWLGTHRFYVDERVI
                     VPRSFIAELLENWCQRPISEAPAGRRSPPKRVSDTSFQILDLCTGSGCLAIVAALAFP
                     RATVDAADISADALEVAKRNVADYGLQARVRLVKSDLFAALEGRAYALVISNPPYVKA
                     ASMKALPDEYRKEPRIALASGADGLDHTRRILDEAFGHMNPGGTLVVEIGRNRKALER
                     ACPELPFEWPATRAGRGFVFALPREALRGKA"
     gene            complement(1502..>2158)
                     /gene="dapE"
                     /locus_tag="VF309_00740"
     CDS             complement(1502..>2158)
                     /gene="dapE"
                     /locus_tag="VF309_00740"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX7465135.1"
                     /translation="IVGEPSSVHVFGDTIKNGRRGSLTGRLVVHGVQGHVAYPQRAKN
                     PVHLVAPALAEVATTEWDRGNEYFPPTTWQISNFHAGTGAQNIIPGSAQVDFNFRFST
                     ASTPEGLKARVAEILDRHGLEYDLEWTLGGKPFLTQRGRLVETLSRAVQKVSGVTPEI
                     NCTGGTSDGRFIIDICPEVAEFGPVNRSIHKVNEAVALEEIEPLAEVYRVAVEDLLRG
                     "
BASE COUNT          334 a          713 c          759 g          352 t
ORIGIN      
        1 ggtggcgcgt tcgtcgcgcg gcccacagcg cgagcgccgt caccggaaag agggacagca
       61 gagcgcccac cgtgggagtg agctcgtcga tgatcgcgaa gtgggcgaga agcgcgaaac
      121 cggccgaaag gagcactgcg agcgcgagcc aggcgcggct gcgtggcgcg cgcgtcactt
      181 cgttctccgc tccgcgacgt gagctgcgag gttgttgagc gagcggaaga tgcgcaggtt
      241 gtcctggtcg tcctccttga gcttgaaccc gtagcgcttc gaaaccacca gggcgacctc
      301 gaggatgtcg atggaatcga ggccgagccc ttcgccgtag agcggcgcgg ccggatcgat
      361 ctcgtccgcc ttcacttcga ggttgagcgc cgaaaccacc aggcctgcga tctcgcgcac
      421 cagggccggg tcggcttcat gcccgttgac cgggacgttt tcgagggaat tcggcatcgt
      481 tttcgagagg gccagacgtg tgaaatgata gcacctggcc tcgcttcgcc tcgcgcgatc
      541 aggccttgcc gcgaagcgct tcccgtggca gggcgaacac gaagccccgg ccggctctcg
      601 tggcgggcca ttcgaacggc agctcggggc aggcgcgctc gagcgccttg cggttgcggc
      661 cgatctcgac gacgagcgtg ccgccaggat tcatgtgtcc aaaggcttcg tcgaggatgc
      721 gccgcgtgtg atcgaggccg tcggcgccgc tcgcgagggc gatgcgcggc tccttgcggt
      781 actcgtcggg cagcgccttc atcgacgcgg ccttgacgta gggcgggttc gagatgacga
      841 gggcgtaagc acggccctcc agcgcggcga agagatccga tttcacgagc ctcacgcggg
      901 cctgcaatcc gtagtccgcg acgttcctct tcgccacttc cagcgcgtcg gccgagatat
      961 cggcggcatc gaccgtcgcc cgcggaaacg cgagcgccgc cacgatcgcg aggcacccgg
     1021 accccgtgca caagtcgaga atttgaaaac tggtgtcaga gacccttttc ggcggagagc
     1081 gccggcccgc cggcgcttcc gaaatgggtc tctggcacca gttttcgagg agctcggcga
     1141 tgaaggagcg cggcactatg acgcgctcat cgacgtagaa gcgatgcgtt ccgagccacg
     1201 cctcgtgcat caggtaggcg gcgggcttgc gggtgcgcac gcgcgcgtcg atgagcgccg
     1261 tggcggcctt cacctgcgcg gcggtgagcg gcgtgtcgag atgcgcgtcg aggttgaccg
     1321 gcggcaagcc cagcgcgtgc aggatgagcc acgccgcctc gtcgagcgcg ttcgcggtcc
     1381 cgtggccgta ggaaagcccc gctttctcga actcgtcgat ggcgtgcgcg acgagcgccc
     1441 ggacggtcct agcgccggcc atcggcgcgc tcaacgacgc ccatcggcgc ttcgcctctc
     1501 gctagccgcg cagcaggtcc tcgaccgcga cacgatagac ctccgcgagc ggctcgatct
     1561 cctcgagcgc gaccgcctcg ttcaccttgt ggatcgatcg gttcacgggc ccgaactccg
     1621 cgacctcggg acagatgtcg atgatgaagc gcccgtcgga cgtgccgccg gtgcagttga
     1681 tctcgggggt gacgcccgag accttctgca ccgcgcggct gagcgtctcc accagccgcc
     1741 cgcgctgcgt gaggaagggc tttccgccga gcgtccactc gaggtcgtat tcgagcccgt
     1801 ggcgatcgag gatttccgcc acgcgcgcct tgagcccctc tggcgtgctt gccgtggaga
     1861 agcggaagtt gaagtccacc tgcgcggagc cggggatgat gttctgcgcg ccggtgcccg
     1921 cgtggaagtt cgagatctgc cacgtggtcg gcgggaaata ctcgttgccg cggtcccact
     1981 cggtggtcgc gacctccgcc agggcgggcg cgacgaggtg caccggattc ttcgcgcgct
     2041 gcggataggc cacgtgcccc tgcacgccgt gcaccacgag ccggccggtg agggagccgc
     2101 gccggccgtt cttgatggtg tcgccgaaca cgtgcaccga cgagggctcg cccacgat
//
LOCUS       DASVII010000059         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_254730,
            whole genome shotgun sequence.
ACCESSION   DASVII010000059 DASVII010000000
VERSION     DASVII010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254730"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3531)
                     /gene="gltB"
                     /locus_tag="VF309_00745"
     CDS             complement(<1..3531)
                     /gene="gltB"
                     /locus_tag="VF309_00745"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX7465136.1"
                     /translation="MNPIAGLPPKQGLYDPRREHDACGVGFVAHIKGRKSHDIVEKGL
                     TILNNLRHRGATGADPLHGDGAGLLVQIPDTFFREEMAKQNVKLPKAGAYGVGMVFLP
                     RNPAARAACVREIERAIANEDQALLGWREVPVDDTSLGPTARSTMPTIRQVFVGAGRG
                     QLDRDAFERKLYVIRKSLGHAIRALGLSNAGDFYVPSFSHRTIVYKGMLLAEQVGAFY
                     SDLNDKRFASALCLAHQRFSTNTFPTWDLAHPFRYIAHNGEINTLQGNLNWIRSRQAT
                     IHSKLLGDDLDKVWPLIYDGQSDSASFDNALELLVMGGYPIAHAMMLMIPEAWAGNPL
                     MDAKRRAFYEYHAALMEPWDGPAAVAFTDGKFIGATLDRNGLRPARYFITDDDLIVMS
                     SEMGVLDVPQEKIIKKWRLQPGKMLLVDIEHGRIVSDEELKRDLAAQRPYQDWLDRTQ
                     IRLEDIPTPWEIPTPDTDKLLDRQQAFGYTQEDLRMLMTPMAIAGEEAIGSMGDDTPL
                     AVLSNRARPLYSYFRQLFAQVTNPPIDPIREQLVMSLVSFIGPRPNLLSPDQTEPVMR
                     LEVSQPILTFEDMEKLRRASTFTQGHFKSEILTMCYPAEWGVDGMEPALAQLCADAED
                     AVRKGTNIVILTDRDLSVTLIPIPALLATAAVHHHLVRAGLRTRCGLVVETGSAREVH
                     HFACLAGFGAEAIHPNLAFATLADIRDGMAQKIDEKELVKRYIKAIGKGLLKVMSKMG
                     ISTYQSYCGAQIFEAVGLATPFVDKYFSGTPTAVDGIGLAEVAEEAVRLHRLAYSDAP
                     LYRDQLDPGGEYAYRTRGEAHMWTPDSIAKLQHATRSGNFATFKEYSRLIDEQGERLL
                     TLRGLFRIKEAPTAVPLDEVEPAKDIVKRFATGAMSLGSISHEAHSTLAIAMNRIGGK
                     SNTGEGGEDPIRYVPLANGDSMRSAIKQVAAGRFGVTTEYLVNADDLQIKMAQGAKPG
                     EGGQLPGHKVSKYIAKVRHTVPGVGLISPPPHHDIYSIEDLAQLIHDLKNVNPKARVS
                     VKLVSEIGVGTVAAGVAKAKADHVTISGHDGGTGASPLSSVKYAGSPWEIGLAETQQT
                     LVLNKLRGRIAVQADGQMKTGRDVVIGALLGADEFGFATAPLVVQGCIMMRKCHLNTC
                     PVGIATQDPELIKKFEGKPEHVINYFFFIAE"
BASE COUNT          535 a         1178 c         1255 g          651 t
ORIGIN      
        1 ctcggcgatg aagaagaaat agttgatgac gtgctcgggc ttgccctcga atttcttgat
       61 gagctcggga tcctgggtgg cgatgcccac ggggcacgtg ttgaggtggc acttgcgcat
      121 catgatgcag ccctgcacga ccaggggcgc cgtggcgaag ccgaactcgt cggcgccgag
      181 gagcgcgccg atcacgacgt cgcgcccggt cttcatctgc ccatccgctt gtaccgcgat
      241 gcgcccgcgc agcttgttca gcaccagcgt ctgctgcgtt tccgcgagtc cgatctccca
      301 cgggctcccg gcgtacttga ccgacgacag cggcgacgcg ccggtccccc cgtcgtggcc
      361 cgagatggtg acgtgatcgg ccttcgcctt cgccacaccc gccgcgacgg tgccgacgcc
      421 gatctcgctc acgagcttca ccgacacgcg cgccttcggg ttcacgttct tgaggtcatg
      481 gatgagctgc gcgagatcct cgatcgagta gatgtcgtga tggggcggcg gcgagatgag
      541 gcccacgccc ggcacggtgt ggcgcacctt ggcgatgtac ttcgagacct tgtgcccggg
      601 aagctgcccg ccctcgccgg gcttcgcgcc ctgcgccatc ttgatctgca ggtcgtcggc
      661 gttgaccagg tactcggtcg tgacgccgaa gcgtcccgcg gccacctgct tgatcgccga
      721 gcgcatcgag tcgccgttgg cgagcggcac gtagcggatg ggatcctcgc cgccctcgcc
      781 cgtgttggac ttgccgccga tgcggttcat cgcgatggcg agcgtcgagt gcgcctcgtg
      841 cgagatcgac ccgagggaca tcgccccggt cgcgaagcgc ttgacgatgt ccttcgcggg
      901 ctcgacttcg tcgagaggca ccgccgtcgg cgcctccttg atcctgaaga ggccgcgcag
      961 cgtgaggagc ctctcgccct gctcgtcgat gagcctcgag tattccttga acgtcgcgaa
     1021 attgccggag cgcgtcgcgt gctgcagctt cgcgatcgag tcgggcgtcc acatgtgcgc
     1081 ttcgccgcga gtgcggtacg cgtattcccc gcccgggtcg agctggtcgc ggtatagcgg
     1141 cgcgtccgag taggcgaggc gatgcaggcg caccgcttcc tcggcgactt ccgcgaggcc
     1201 gatgccgtcg accgccgtgg gcgtgccgct gaagtacttg tcgacgaagg gcgtcgcaag
     1261 ccccacggcc tcgaagatct gcgctccgca gtaggactgg tacgtcgaga tgcccatctt
     1321 cgacatgacc ttgaggaggc ccttgccgat cgccttgatg tagcgcttga cgagctcctt
     1381 ctcgtcgatc ttctgcgcca tgccgtccct gatgtcggcg agcgtcgcga acgcgagatt
     1441 ggggtggatc gcctcggcgc cgaatcccgc gaggcacgcg aagtgatgca cctcgcgcgc
     1501 gctcccggtc tcgacgacga ggccgcagcg cgtgcgcagg cccgccctca ccaggtggtg
     1561 atgcaccgcg gcggtggcga ggagcgccgg gatcggaatg agcgtcacgg acaagtcgcg
     1621 gtccgtgagg atgacgatgt tcgtgccctt gcgcaccgcg tcctcggcat cggcgcagag
     1681 ctgcgcgagc gccggctcca tgccatccac gccccactcg gcgggatagc acatcgtgag
     1741 gatctcgctc ttgaagtggc cttgcgtgaa ggtcgacgcg cggcgcagct tctccatgtc
     1801 ctcgaaggtg aggatcggct gcgagacctc gaggcgcatc acgggctcgg tctggtccgg
     1861 cgacagcagg ttcgggcgcg ggccgatgaa cgacacgagc gacatcacga gctgctcgcg
     1921 gatcggatcg atcggcggat tggtcacctg cgcgaagagc tggcggaaat acgagtagag
     1981 cggcctcgcc cggttcgaca ggaccgcgag cggcgtgtcg tcgcccatgg agccgatcgc
     2041 ctcctcgccg gcgatcgcca tcggcgtcat cagcatgcgc aggtcttcct gcgtgtagcc
     2101 gaacgcctgc tggcggtcta ggagcttgtc cgtgtccggc gtcggaattt cccagggcgt
     2161 gggaatgtcc tcgaggcgga tctgcgtgcg gtcgagccag tcctggtagg ggcgctgcgc
     2221 ggcgaggtcg cgcttcagct cctcgtcgga gacgatgcgg ccgtgctcga tgtcgaccag
     2281 cagcatcttg cccggctgca ggcgccactt cttgatgatc ttttcctgcg ggacgtcgag
     2341 gactcccatc tccgaggaca tgacgatgag gtcgtcgtcg gtgatgaagt agcgcgccgg
     2401 gcgcaagccg ttgcgatcga gcgtggcgcc gatgaacttg ccgtcggtga acgcgaccgc
     2461 ggcgggcccg tcccacggct ccatcagggc cgcgtgatat tcgtagaacg cgcggcgctt
     2521 cgcgtccatc agcgggttgc ccgcccacgc ctcgggaatc atcagcatca tcgcgtgcgc
     2581 gatcggatag ccgcccatca cgaggagctc gagcgcgttg tcgaacgacg ccgagtccga
     2641 ctggccgtcg tagatgagag gccagacctt gtcgaggtcg tcgcccagca gcttcgagtg
     2701 gatcgtcgcc tgccgcgagc ggatccagtt caggtttccc tggagcgtgt tgatctcgcc
     2761 gttatgcgcg atgtagcgga agggatgcgc gaggtcccac gtcgggaacg tattcgtcga
     2821 gaagcgctgg tgcgcgaggc acagggcgga ggcgaagcgc ttgtcgttca agtcgctgta
     2881 gaaggcgccc acctgctcgg cgaggagcat ccccttgtag acgatggttc gatgcgagaa
     2941 cgacggcacg tagaagtcgc cggcgttcga gaggcccagg gcgcggatcg cgtggccgag
     3001 gctcttcctg atgacgtaga gcttgcgctc gaacgcgtcg cggtcgagct ggccgcggcc
     3061 ggccccgacg aagacctggc ggatcgtggg catcgtgctg cgcgccgtcg ggccaagcga
     3121 cgtgtcgtcc acggggactt cgcgccaccc gaggagcgcc tggtcctcgt tggcgatggc
     3181 gcgctcgatc tcgcgcacgc aggccgcgcg cgccgcgggg ttgcgcggca ggaacaccat
     3241 gcccacgccg taggccccgg ccttcggcag cttcacgttc tgcttcgcca tctcctcgcg
     3301 gaagaacgtg tcggggatct gcaccagcag gcccgcaccg tcgccgtgca ggggatcggc
     3361 ccccgtcgca ccgcgatggc gcaggttgtt caggatcgtg aggccctttt cgacgatgtc
     3421 gtgggacttc cggcccttga tgtgcgcgac gaagccgacg ccgcacgcgt cgtgctcgcg
     3481 gcgtgggtcg tagagtccct gcttcggcgg gaggcccgcg atcggattca tggagtcacc
     3541 tgtcgttcag tcctgtcccc gcgccatggg gaccccagcg caaatggtag ccgcgttgcc
     3601 tctatcttcg ggggagcgc
//
LOCUS       DASVII010000060         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_398670,
            whole genome shotgun sequence.
ACCESSION   DASVII010000060 DASVII010000000
VERSION     DASVII010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398670"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF309_00750"
     CDS             complement(<1..217)
                     /locus_tag="VF309_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465137.1"
                     /translation="MTSPEPRHDLDMGLPQQRLFFVLGPVVGVVSFSALLALASALMG
                     FHRDEMMVIAAAAGAAVALPALLLLYRQ"
     gene            331..1701
                     /gene="hemN"
                     /locus_tag="VF309_00755"
     CDS             331..1701
                     /gene="hemN"
                     /locus_tag="VF309_00755"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX7465138.1"
                     /translation="MIDAHLLRKYGGNGPRYTSYPTADRFVEAFDARAYRHWLANRSI
                     GGFARPLGLYVHVPFCDTLCFYCACNKIATRDHAKAHRYVGYLAREAQLVAEAMGDDR
                     RVTRIHWGGGTPTFLGDEESERLMTALRSSFELDPDGEYAIEVDPRRVAPERIAFLGR
                     LGFNRISVGVQDFDPAVQLAVNRIQSLEETRALIDAARANGYKSVNIDLIHGLPKQTV
                     AGFAATLERVLECAPDRIALYSYAHLPAVFKPQRRIHEADLPSGEAKLELMTSAIRRL
                     ERAGYVHIGMDHFARPGDELAVAQRQGRLIRDFQGYSTGGDRDVVGLGVSAIGKIGPS
                     YAQNEKALDDYYAVLDGGELPILSGIELKPDDIARRAVIQALACDLAVSKTSIGIAHL
                     LDFDTYFAAELEELRGLEADGLVEFDDEWIHVTPRGRLLVRAVCMVFDRYLRETRERT
                     RYSRVM"
     gene            1698..>1894
                     /locus_tag="VF309_00760"
     CDS             1698..>1894
                     /locus_tag="VF309_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7465139.1"
                     /translation="MIASLAAAAFAAGLLGGVHCAGMCGGIVAGLSASARGPLLARQL
                     QFNGARIASYGVVGAAAGLAG"
BASE COUNT          297 a          676 c          645 g          276 t
ORIGIN      
        1 cctggcgata gagaaggagg agggcgggga gcgccacggc ggcgccggcg gccgcggcga
       61 tcaccatcat ctcgtcgcgg tggaagccca tcagcgccga ggccagcgcc agaagcgcgc
      121 tgaaggagac caccccgaca accggcccca atacgaagaa caggcgctgc tgcggcagcc
      181 ccatgtcgag atcgtgccgc ggctccgggg acgtcatgca gggggtttga cgggggtcaa
      241 ggcacgcgcg cgaacgccgc gcatagtgcg ggcatgaaca tcgccgcgat ttccgccccg
      301 cctgttggcg cgccccacga ttctcccttg gtgatcgacg cgcatttgct gcgcaagtac
      361 ggcggcaacg gcccacgcta cacgtcgtac ccgaccgccg atcgcttcgt cgaggccttc
      421 gacgcgaggg cctaccgcca ctggctcgcc aaccgctcga taggcggctt cgcgagaccc
      481 ctcggattat acgtgcacgt ccctttctgc gacacgctgt gcttctactg cgcgtgcaac
      541 aagatcgcca cgcgcgacca cgcgaaggcg caccgctacg tgggctacct cgcgcgcgag
      601 gcgcagctgg tggccgaggc gatgggcgac gaccgccgcg tgacgcgcat ccattggggc
      661 ggcggcacgc ccactttcct cggcgacgag gagtcggagc gcctcatgac ggcgctgcgc
      721 tcgagcttcg agctcgatcc cgacggcgaa tacgcgatcg aggtcgaccc gcggcgcgtg
      781 gcgcccgagc gcatcgcctt cctcggccgc ctgggcttca accgcatcag cgtgggcgtt
      841 caggatttcg accccgcggt gcagctggcg gtgaaccgca tccagagcct ggaagagacg
      901 cgcgcgttga tcgacgcggc gcgcgccaac ggatacaagt cggtcaacat cgacctcatc
      961 cacggcctgc ccaagcagac ggtcgcggga ttcgcggcga cgctcgagcg cgtgctcgaa
     1021 tgcgccccgg atcgcatcgc gctctattcg tacgcgcacc tgccggcggt cttcaagccg
     1081 caacggcgca tccacgaggc cgacctgccc agcggcgagg cgaagctcga gctcatgacc
     1141 tccgcgatcc ggcgcctcga gcgcgcgggc tacgtgcaca tcggaatgga ccatttcgcg
     1201 cgtcccggcg acgagctcgc ggtggcgcaa cgccaggggc gcctcatccg cgacttccag
     1261 ggctattcca ccggcggcga ccgcgacgtg gtcggcctgg gcgtctcggc gatcggcaag
     1321 atcggcccca gctacgcgca gaacgagaag gcgctcgacg attactacgc cgtgctcgat
     1381 ggcggagagc ttcccatcct gagcggcatc gagctcaagc ccgacgacat cgcgcgccgc
     1441 gccgtgatcc aggcgctcgc ctgcgacctc gcggtgtcga agacctcgat cggcatcgcc
     1501 cacctcctcg atttcgacac ctacttcgcc gccgagctgg aagagctgcg cggcctcgag
     1561 gccgacggcc tggtggagtt cgacgacgag tggatccacg tcacgccgcg cgggcgcctg
     1621 ctggtgcgcg cggtgtgcat ggtattcgac cgctacctgc gcgagacgcg cgagcgcacg
     1681 cgctactcgc gcgtcatgtg atcgcgtctc tcgccgccgc ggccttcgcc gcggggctcc
     1741 tcggcggcgt gcattgcgcg ggaatgtgcg gcggcatcgt cgccgggctt tcggccagcg
     1801 cgcgcggccc gctcctcgcg cgccagcttc aattcaatgg cgcacgcatc gcgagctacg
     1861 gcgtggtggg ggcggcagcc ggcctcgcgg ggaa
//
LOCUS       DASVII010000061         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_398699,
            whole genome shotgun sequence.
ACCESSION   DASVII010000061 DASVII010000000
VERSION     DASVII010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398699"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VF309_00765"
     CDS             <1..431
                     /locus_tag="VF309_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465140.1"
                     /translation="LWQSNVEHTSEQFRNERKVITHEVFKVKCDFRVQAKVDGDHESG
                     KLKIVAKNVNGFSVDLFNLMASEMNDQGVEEFAKHFIGRPNHWADLVKRSVLYPRPSA
                     AAAPKPKTATNYVVQKTPEPPPAEEKRGLLGSLKSIIKKD"
     gene            462..1589
                     /locus_tag="VF309_00770"
     CDS             462..1589
                     /locus_tag="VF309_00770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7465141.1"
                     /translation="MPRLLVLVGTRKGLFILSSRADRREWSIDGPHFLGQVVNHAVLD
                     PRDGRTLLAAVRAGHLGPTAYRSEDLGKTWTEARIPPAFRKAAEGERGEAVSHVFWLT
                     PGHASQPSRWYAGVSPPGLFVSGDAGRNWKGVAGFNENPDRIEWCGGLQDAPPDGATL
                     HSVNVDPFDADHLYIGISTGGVFESRDAGATWSPLNKGVAADFLPDPDAPFGHDPHCL
                     RVHGAARDVVYQQNHCGIYRLDRGADRWQRIGEHMPREIGDIGFPLTLHPRDPKTLWV
                     FPMDGGTVWPRTAIGGKPAVYESRDAGESWKRRDGGLPREQAWFTVKRQAMTSDALDP
                     VGLYFGTTGGELWASFDEGGAWQCLRMHLPHIYSVETAIVP"
     gene            1586..1846
                     /locus_tag="VF309_00775"
     CDS             1586..1846
                     /locus_tag="VF309_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7465142.1"
                     /translation="MKVLLPTPLADYTAQAREVEAEGATLAELLDDLDRRFPGIRFRM
                     IDEQGAIRAHIKIFVNRRQVPGIASRLEPSDEVLVVAALSGG"
BASE COUNT          325 a          668 c          647 g          305 t
ORIGIN      
        1 tgctgtggca gtcgaacgtc gagcacacca gcgagcagtt ccgcaacgag cgcaaggtga
       61 tcacccacga ggtcttcaag gtcaagtgcg atttccgcgt gcaggcgaag gtcgacggcg
      121 atcacgagtc gggcaagctc aagatcgtcg cgaagaacgt gaatggattc agcgtcgacc
      181 tcttcaacct catggcgtcg gaaatgaacg accagggcgt ggaggaattc gcgaagcact
      241 tcatcggccg ccccaaccac tgggccgacc tggtgaagcg ctcggtgctc tacccgcgtc
      301 cgtccgccgc ggccgcgccg aaacccaaga ccgcgaccaa ttacgtcgtc cagaagacgc
      361 cggagccccc gcccgccgag gaaaagcgcg gcctgctggg ctccctgaag tcgatcatca
      421 agaaagacta gcggcgcgcg tcttcccgcc cgagagcggc catgccccgg ctcctggtcc
      481 tggtaggcac ccgcaagggc ctcttcatcc tttcgtcgcg cgccgatcgg cgcgagtggt
      541 cgatcgacgg cccccatttc ctcggccagg tcgtgaacca cgcggtgctc gacccgcgtg
      601 atggccgaac gctgctcgcg gcggtgcgcg cgggccatct cgggccgacc gcctaccgtt
      661 cggaggacct cggaaagacg tggaccgagg cgcgaatccc tcccgcgttc cgcaaggccg
      721 ccgagggcga gcgcggcgag gccgtgagcc atgtgttctg gctcacgccc gggcacgctt
      781 cgcagccctc gcgctggtac gcgggagtct cgccgccggg cctcttcgtt tccggcgacg
      841 cggggcgcaa ctggaagggc gtcgcgggat tcaacgagaa tcccgatcgc atcgaatggt
      901 gcggcggcct gcaggatgcg ccacccgatg gcgcgacgct ccattccgtg aacgtcgatc
      961 cgttcgacgc cgatcacctc tacatcggga tctccacggg aggcgtgttc gaatcgcgcg
     1021 acgcgggcgc gacatggtcg ccgctcaaca agggtgtcgc ggcggacttc cttcccgatc
     1081 ccgacgcccc cttcgggcac gatccccatt gcctgcgcgt gcatggcgcg gctcgcgacg
     1141 tcgtgtacca gcagaaccac tgcggcatct atcggctcga ccgcggcgcc gaccgctggc
     1201 agcgcatcgg cgagcacatg cccagggaaa tcggcgacat tggttttccg ctcacgctgc
     1261 acccgcgcga tccgaagacg ctctgggtct tcccgatgga tggcggcacc gtatggccgc
     1321 gcaccgcgat cggcggcaag cccgcggtgt acgagtcgcg ggacgcgggc gagtcgtgga
     1381 agcgccggga tggcggactg ccccgggagc aggcctggtt cacggtgaag cgccaggcga
     1441 tgacgagcga cgcgctcgat cccgtggggc tctacttcgg cacgacgggc ggcgagctct
     1501 gggcgagctt cgacgagggc ggcgcctggc agtgcctgcg catgcacctg ccgcacatct
     1561 actcggtcga gaccgcgatc gttccgtgaa ggtcctgttg cccacgccgc tggccgacta
     1621 cacggcgcaa gcccgcgagg tcgaggccga gggagcgacg ctcgccgagc ttctcgacga
     1681 tctcgaccgc cgcttccccg gcattcgctt ccgcatgatc gacgagcagg gcgcgatccg
     1741 cgcccacatc aagatcttcg tgaaccggcg gcaggttccg ggaatcgcgt cgcgattgga
     1801 gccatcggac gaggtcctgg ttgtcgcggc tctgtcagga ggctgatgta ccatggccgc
     1861 acaaatcgag cctgaggaga gacctttcat gaccgccaaa tccctcttcg cgtccctcgc
     1921 gctgagtatc gcggcgaccg cggcg
//
LOCUS       DASVII010000062         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_75050, whole
            genome shotgun sequence.
ACCESSION   DASVII010000062 DASVII010000000
VERSION     DASVII010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75050"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VF309_00780"
     CDS             complement(<1..302)
                     /locus_tag="VF309_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX7465143.1"
                     /translation="MTPAPAPRQREVTPSPSPQERLEAVERVIGRLHREVGPPYDRAT
                     AAREVHELTEELHPADIAHILEGLPVEDRLWVWGLVGPDREGDVLLEVSDAVRDTLL"
     gene            complement(299..994)
                     /gene="mtgA"
                     /locus_tag="VF309_00785"
     CDS             complement(299..994)
                     /gene="mtgA"
                     /locus_tag="VF309_00785"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX7465144.1"
                     /translation="MKRALRFAAWLAVAAAAVFLAVQLSFLARVWWWKDHDPPTTAFM
                     DARLEAMRAREPQAKLQHAWVAYDRISANLKRAIVVAEDSKFVDHEGFDWEAIEKARE
                     KNRRKGKVVAGGSTISQQLAKNLFLSGARTPWRKGQEALITVMIEHMMDKRRILEIYL
                     NVIEWGEGVFGAEAAARHYYGVSALALGPEPAARLAAMVPNPRFYDRNRGSAWLARKT
                     QIILARMPAAELP"
     gene            complement(991..1806)
                     /gene="aroE"
                     /locus_tag="VF309_00790"
     CDS             complement(991..1806)
                     /gene="aroE"
                     /locus_tag="VF309_00790"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7465145.1"
                     /translation="MDRYAVIGNPVSHSLSPAIHSHFARQLGESVEYTALLAPLDDFA
                     GSARRFFDGGGRGANVTLPFKLDAFRFAPRASQRALLAGAANFLAVEEQGIRADNTDG
                     IGLVADLRENLGVDLAGSRILILGAGGAARGVIAPLLEAGPLRVVIANRTMERARELA
                     QRFAAEGRVEAVALDAIPKEEFDLVLNATSTSIHGETLALPAHVLRAGALAYDMAYGP
                     GAAAFLARARAQGMRASDGLGMLVEQAAESFRLWRGKRPATAEILAELRSRSA"
BASE COUNT          287 a          644 c          602 g          273 t
ORIGIN      
        1 aggagcgtgt cgcgcacggc atcggacacc tcaaggagca cgtcgccttc gcggtcgggc
       61 ccgacgaggc cccacaccca gaggcggtcc tccaccggga ggccctcgag gatgtgcgcg
      121 atatccgcgg gatggagctc ctcggtgagc tcatgcacct ctcgcgcggc cgtcgcgcga
      181 tcgtacgggg gacccacctc gcgatgcagc cgcccgatga ctcgctcgac ggcttcgagc
      241 cgttcctgcg gagacggcga gggcgtgacc tcgcgctgcc gcggtgccgg tgcgggcgtc
      301 acggcagctc cgccgcgggc atccgcgcga ggatgatctg cgtcttgcgg gcgagccacg
      361 cgctgcctcg attgcgatcg tagaaacgcg gattgggaac catcgccgcg aggcgcgccg
      421 cgggctcggg cccaagcgcg agcgcgctca ccccgtagta atggcgcgcc gccgcctcgg
      481 cgccgaacac gccctccccc cactcgatga cgttcaggta gatctcgagg atgcgccgct
      541 tgtccatcat gtgctcgatc atcaccgtta tcagcgcttc ctggcccttg cgccacggag
      601 tgcgagcgcc cgagaggaac aggttcttcg cgagctgctg ggagatcgtc gatccgcccg
      661 cgacgacttt gcccttcctg cgattcttct cccgcgcctt ctcgatcgcc tcccagtcga
      721 agccctcatg gtcgacgaat ttcgaatcct cggccaccac gatcgcgcgc ttcaggttcg
      781 cggaaatgcg atcgtacgca acccatgcat gctgcagctt cgcctggggc tcgcgcgcgc
      841 gcattgcctc caggcgcgcg tccatgaacg ccgtggtcgg cggatcgtgg tccttccacc
      901 accagacgcg cgcaagaaag gagagctgga ccgcgaggaa gaccgcggcc gccgcgaccg
      961 cgagccacgc cgcgaagcgc agggcccttt tcacgccgag cgcgaccgaa gttccgccag
     1021 gatctccgca gtagcgggac gcttgccgcg ccacaacctg aacgactccg ccgcctgttc
     1081 gacgagcatg cccaggccgt cggacgccct catgccttgc gcgcgggcgc gggcgagaaa
     1141 ggctgccgcg ccgggaccgt aagccatgtc gtaggcgagc gcgccggccc tcagcacgtg
     1201 ggccggcaac gcgagcgtct ctccatgaat cgaggtggag gtcgcgttca ggaccaggtc
     1261 gaactcctcc ttcggaatgg cgtccagcgc caccgcctcg acgcggccct ccgccgcgaa
     1321 gcgttgcgcg agctcgcgtg cgcgctccat ggtgcgattc gcgatcacca cgcgcagcgg
     1381 tcccgcctcg agcagcggcg cgatcacgcc gcgcgcggcg ccgcccgcac cgagtatgag
     1441 gatgcggctt ccggcgagat ccactccgag gttttccctg agatccgcca cgagtccgat
     1501 gccgtcggta ttgtcggcgc ggatgccctg ctcctccacc gcgagaaaat tcgcggcgcc
     1561 cgcgagcagc gcgcgttgcg aggcgcgcgg cgcgaaccga aacgcatcga gcttgaaggg
     1621 aagggtcacg ttggcgccgc gccccccgcc atcgaagaag cgccgcgccg atcccgcgaa
     1681 gtcgtcgagt ggcgcaagga gcgccgtgta ttcgactgat tcccccaatt ggcgcgcgaa
     1741 gtgcgaatgg atcgcgggcg aaaggctgtg cgagacggga ttgccgatga cggcgtagcg
     1801 atccat
//
LOCUS       DASVII010000063         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_39266, whole
            genome shotgun sequence.
ACCESSION   DASVII010000063 DASVII010000000
VERSION     DASVII010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39266"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF309_00795"
     CDS             <1..343
                     /locus_tag="VF309_00795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX7465146.1"
                     /translation="FFARKVMFVKLSCAFVMMPGGFGTLDELSEVLTLVQTGKIRRVP
                     VILVDSTFWKGLLDWIRGQLIADGMIGAGDPGLIQVIDDPQAIVDAIFGHYHSRGFEP
                     SPAEREVELAL"
     gene            376..726
                     /locus_tag="VF309_00800"
     CDS             376..726
                     /locus_tag="VF309_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEX7465147.1"
                     /translation="MRTLLAATLIAAALSAAAQTGERKLPAGATPLTEPPPPPPMQQA
                     DPAIEPVVTTRHEGDQEIIEYRIKGKLYMQRVTPKHGKPYVLMDHQGDGTFMRQDNPL
                     DSGVRVPQWVLKEF"
     gene            747..1694
                     /locus_tag="VF309_00805"
     CDS             747..1694
                     /locus_tag="VF309_00805"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7465148.1"
                     /translation="MSVFTPVSIAEARALLEHYTLGEVERLEGIAQGVENTNYFLTTT
                     TGEYVLTLFEHIPRADLPFYVGLMDHLAHREVTCPAPMRLDNGQLIAEVNGKPACIVT
                     KLPGAPRARPDAHLCRRAGEILARIHVAAVDYDASLDNWRGREWRESFAQALKPKLSR
                     PENDLIAAENRYQGIHDDTVLPRGIIHGDYFHDNVLWDDEGAGGVIDFYFACDDALLY
                     DVAIGVNDWCVNPDATLDTARTHAFIEGYQSQRPLEPLERDLWPVMLRRAALRTWLGR
                     LGYNYFPRDSHMTIPKDHEFSRRLLEHHVAHARALGETL"
BASE COUNT          299 a          595 c          542 g          264 t
ORIGIN      
        1 cttcttcgcg cgcaaggtca tgttcgtgaa gctgtcgtgc gcgttcgtga tgatgccggg
       61 gggcttcggc acgctggacg agttgtcgga agtgctcacc ctcgtgcaga ccgggaagat
      121 ccgcagggtc ccggtgatcc tcgtggattc gaccttctgg aaggggctcc tcgactggat
      181 tcgcggccag ctcatcgccg atggaatgat cggcgccggc gatccggggc tcatccaggt
      241 gatcgacgat ccccaggcga tcgtggacgc gatcttcggc cactatcatt cgcgcggatt
      301 cgagcccagc cccgcggaac gagaagtgga gctcgcgctc taactggtac acttccgacc
      361 cgaatcgaga ctgccatgcg aacacttctt gccgcaacgc tcatcgccgc ggcgctttcg
      421 gctgccgcgc aaaccggcga gcgcaagctt cccgcgggcg ccacgccgct gaccgagccg
      481 cccccgccgc cgcccatgca gcaggccgac ccggccatcg agcccgtcgt gaccacgcgc
      541 cacgagggcg accaggagat catcgagtac cgcatcaagg gcaagctcta catgcagcgc
      601 gtgacgccca agcacggcaa gccgtacgtg ctgatggatc accagggcga cgggactttc
      661 atgcgccagg acaatccgct cgactcgggg gtgcgcgtgc cgcagtgggt cctgaaggaa
      721 ttctagaaac ataaaaaagg ggaggcatgt cggtcttcac gccggtttcg atcgccgagg
      781 cgcgcgcgct ccttgagcac tacacgctgg gcgaggtgga gcggctcgag ggaatcgcgc
      841 agggcgtcga gaacaccaac tacttcctca cgaccaccac gggcgaatac gtgctcacgc
      901 tcttcgagca cattccccgc gcggacctgc cgttctacgt gggcctcatg gaccatctcg
      961 cgcaccgcga ggtgacatgc cccgccccga tgcgcctcga caacggccag ctcatcgcgg
     1021 aagtgaacgg caagcccgcg tgcatcgtga cgaagcttcc cggcgcgccg cgggcgcggc
     1081 ccgacgccca cctgtgccgg cgcgccggcg agatcctggc gcgcatccac gtcgccgcgg
     1141 tcgactacga cgcctcgctc gacaactggc gcgggcgcga atggcgcgag agcttcgcgc
     1201 aggcgctgaa gccgaagctg tcgcgcccgg aaaacgacct catcgccgcg gagaaccgct
     1261 accagggcat ccacgacgac acggtgcttc cccggggcat catccacggc gactacttcc
     1321 acgacaacgt gctctgggac gacgaaggcg cgggcggcgt catcgatttc tacttcgcct
     1381 gcgacgacgc gctcctctac gacgtcgcga tcggcgtgaa cgactggtgc gtgaacccgg
     1441 acgcgacgct cgacacggcc cgcacgcacg ccttcatcga gggctaccag tcgcagcgcc
     1501 cgctcgagcc cttggagcgc gatctctggc cggtgatgct gcggcgcgcg gcgctgcgca
     1561 cgtggctggg gcgcctgggc tacaactatt ttccgcgcga ttcccacatg acgattccga
     1621 aggatcacga gttctcgcgg cggctcctcg agcaccacgt cgcccacgcc cgcgccctcg
     1681 gcgaaacgct ctaggcgatg
//
LOCUS       DASVII010000064         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_326900,
            whole genome shotgun sequence.
ACCESSION   DASVII010000064 DASVII010000000
VERSION     DASVII010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326900"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF309_00810"
     CDS             <1..272
                     /locus_tag="VF309_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465149.1"
                     /translation="VPSDWVSASLAAHIPTGDGRQYGYQWWVGSAPWRDKVIAWSGAI
                     GNGGQRLYLLPELDMAIVITAGEYNSADVGQKLGRLMARIAAAVQ"
     gene            311..1384
                     /locus_tag="VF309_00815"
     CDS             311..1384
                     /locus_tag="VF309_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7465150.1"
                     /translation="MSFEAYQGTRPVAEAHRFDIAALERYMREHVPGFGGTLAVEQFK
                     GGQSNPTYRLSAGGKSYVMRRKPPGKLLPSAHAVDREYRVITALAATDVPVARTYALC
                     TDESVIGSMFYIMDCVEGRVLWDPQLPGMTPAQRGAIFDEMNRVIAALHRVDYAAIGL
                     ADYGKPGNYFARQVDRWSRQYKASETEKIEAMDRLMQWLPSNIPASDETTIVHGDYRL
                     DNMVFHATEPRVLAVLDWELSTLGHPMADFSYHLMTWRLSATEFRGLRGADLAALGIP
                     SEREYIDKYIRRVGKKDPPSEKDLGFCMAYNMFRAAAIFQGVMARAVAGNAASAQAVE
                     TGKRARPMAELGWKQVERLLAQG"
     gene            1395..>1888
                     /locus_tag="VF309_00820"
     CDS             1395..>1888
                     /locus_tag="VF309_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7465151.1"
                     /translation="MDFDYSPKVRDLQSRLKAFMDANIYPNEAAFHEEVAEGDRWQPT
                     RLIEELKVKARAAGLWNLFLPESTRGAGLTNTEYAPLCEIMGRTYWAPEVFNCNAPDT
                     GNMEVLERYGSPEQKKEWLEPLLEGKIRSGFAMTEPAVASSDATNIQASIVRDGGNYV
                     LNGR"
BASE COUNT          340 a          623 c          636 g          289 t
ORIGIN      
        1 tcgtcccgtc cgattgggtg tcggcttccc tcgccgcgca catccccacg ggcgacgggc
       61 ggcaatacgg ataccaatgg tgggtcggaa gcgcgccgtg gcgcgacaag gtgatcgcgt
      121 ggagcggggc catcggcaac ggcggccagc gcctctacct gctgccggag ctggacatgg
      181 ccatcgtgat caccgcgggc gaatacaaca gcgccgacgt cggacagaag ctcggccggc
      241 tgatggcgcg aatcgccgcg gcggtgcagt agtatcccta ttccgcagtc cgattcctcc
      301 aagcctgccc atgagtttcg aggcctacca aggcacgcgt ccggtcgccg aagcccaccg
      361 cttcgacatc gcggcgctcg agcgctacat gcgcgagcat gtgccgggct tcggcggcac
      421 gctcgccgtc gagcagttca aggggggcca atccaatccg acctaccggt tgagcgcggg
      481 cggcaagtcg tacgtgatgc gccgcaagcc cccggggaaa ctgctgccgt cagcccacgc
      541 ggtggaccgc gagtaccggg tgatcaccgc gctcgccgcg accgacgtgc ccgtcgcgcg
      601 cacctacgcc ctgtgcaccg acgagtcggt gatcggctcg atgttctaca tcatggattg
      661 cgtcgagggc cgcgtcctgt gggatccgca gctgccgggc atgactccgg cgcagcgggg
      721 cgcgatcttc gacgagatga atcgcgtcat cgccgcgctg catcgcgtcg actacgcggc
      781 catcggcctt gccgattacg gcaagccggg caactacttc gcgcgccagg tcgatcgctg
      841 gtcgcggcag tacaaggcgt ccgagaccga gaagatcgag gcgatggatc gcctcatgca
      901 gtggctgccg tcgaacattc ccgcgagcga cgagaccacg atcgtccatg gcgactaccg
      961 cctcgacaac atggtgttcc atgccaccga gccgcgcgtg ctcgccgtgc tcgactggga
     1021 gctctcgacc ctcggccatc cgatggcgga cttctcctac catttgatga cctggcggct
     1081 gtcggcgacg gaattccgtg ggctgcgcgg cgccgacctc gcggcgctgg gaattccgag
     1141 cgagcgcgaa tacatcgaca agtacatccg gcgcgtgggg aagaaggatc cgccgagcga
     1201 gaaggacctg ggcttctgca tggcgtacaa catgttccgc gccgcggcga tcttccaggg
     1261 cgtgatggcg cgcgcggtcg cgggcaacgc ggcgagcgcg caggcggtgg aaacgggaaa
     1321 gcgcgcgcgg ccgatggccg agctcggttg gaaacaggtc gagcgactgc tcgcccaagg
     1381 atagggagac gacgatggac ttcgattact cgccgaaggt gcgcgacctg caatcgcgcc
     1441 tgaaggcctt catggatgcg aacatctatc ccaacgaagc ggccttccac gaggaagtcg
     1501 ccgagggaga ccgctggcag ccgacgcgcc tcatcgagga gctgaaggtg aaggcgcgcg
     1561 cggcgggatt gtggaacctc ttcctgcccg aatccacgcg cggcgccggg ctcaccaaca
     1621 ccgagtacgc gccgctttgc gagatcatgg gccgcaccta ctgggccccg gaagtcttca
     1681 actgcaacgc gcccgatacc ggcaacatgg aggtgctcga gcgctacggc tcgcccgagc
     1741 agaagaaaga gtggctcgag ccgctcctcg agggaaagat ccgctcgggc ttcgcgatga
     1801 ccgagcccgc ggtggcgtct tcggacgcga ccaacatcca ggcttccatc gtgcgcgatg
     1861 gcggcaacta cgtgctcaac ggccgcaa
//
LOCUS       DASVII010000065         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_255031,
            whole genome shotgun sequence.
ACCESSION   DASVII010000065 DASVII010000000
VERSION     DASVII010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255031"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..504
                     /locus_tag="VF309_00825"
     CDS             295..504
                     /locus_tag="VF309_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465152.1"
                     /translation="MKNIIAIGLACCIPIGFATGDSNPSEANLGSMAVLVVDDSAPGG
                     ADLQRAVLTARTQEVSVLHERGRWM"
     gene            525..755
                     /locus_tag="VF309_00830"
     CDS             525..755
                     /locus_tag="VF309_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7465153.1"
                     /translation="MNSDQMHGHWREVKGWALEQLGRVTQDPWKRIAGRRERLLGRLQ
                     ASLAESRHIAESSFPASQTGAILVERRRTREL"
     gene            complement(760..1674)
                     /locus_tag="VF309_00835"
     CDS             complement(760..1674)
                     /locus_tag="VF309_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7465154.1"
                     /translation="MQSRSAGHLMRDGQGMATPPRGGAEGELATPSLAHAAEELVRAA
                     GGSSLQWEGASLTTHFQPIYCVRRAACLGFEALVRASDESGTTVSPEELFARAPDGAR
                     SLLDWTCRALHLRNYATVDPGDRTLFINIHPEAAVRDARRGREFAELIRYYGLVPKRV
                     CVEILEAPCSSEKLLREAVECYRGLGASIAMDDFGVGCSNFDRIVALRPDVVKVDRSI
                     LAGVVGNEKARRMLPGMIELLHEFQCKVAVEGIESRAAALLAIEAKADYLQGYFFAGP
                     QSRLADEFDGAALLDKLLRASPGSRLAA"
     gene            complement(1779..>3603)
                     /locus_tag="VF309_00840"
     CDS             complement(1779..>3603)
                     /locus_tag="VF309_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7465155.1"
                     /translation="TVPGFIPPLIYLCAPFFIWAALRFGLRAATLGLSIFGLICYWQT
                     AHGFGPFAVDGFADARSMLHLQGYLATIVVTTLFSAALLVERQDAARETEGWRARHEA
                     VIRASGNLLYELNLDDGKVIWDGDTQSVLGVGHDAISDLRKWMERVHPDDRIRLKGLR
                     QRLRQREFSHVALEYRFRRDDGVYTTLGVNAYRIGDPDAEAPGTRRVIGFVKDVSDKV
                     HAEEERVRLEAQLKQAEKMQAVGHLAGGIAHDFNNILGAILGYGELAQRRAAGDGDMK
                     RYLDTIMGAGNRAKALVTQILSYSRAEGAEKFPVIMAPIAQEVVDLVRGSTPLEIDVT
                     FADQCAEATILGDPTRMHQLLMNLCTNAIHSMGQGGTLEVGLACETVAAPRKVRSGEI
                     PPGEYVRVWVKDTGHGIAPEVIDRIFEPFFTTKPAGRGTGLGLALVHSVVSEHKGFIE
                     VASELGRGTTFTVWIPQIHAEEGAADELETLPMGAGQVILAVDDELDVLHALEEMLAQ
                     LGYEPVGFGSSREALEAMRAHPRRFDAVVSDEVMPELIGTQLAKELRKLNPTLPVVIA
                     SGYGGAGFEARALSAGVNRVLRKPYRMREIAEALAIFFAKA"
BASE COUNT          568 a         1244 c         1239 g          552 t
ORIGIN      
        1 gagagcaaga acgcgaagaa ccgcgacctg ggctcgggcc tcgagcacct cgagatcgtc
       61 aagtgaccgc gcggtaggaa gccaggcgat ccgccagccg atcatcccgc gaaggcgggg
      121 acccgatcca tgaaagcccg cctcgcgcgg gctttcttct tttccggccc cttcccgcgc
      181 ctgtaggaac catccgacgc gactatcagc ggattcttaa aaccgatccc cttccgtccg
      241 actccccgcc cgaatccgca tggtcaatat ggacgtaaga caagggagac ggccatgaag
      301 aacatcatcg cgataggttt ggcctgctgc attccgatcg gattcgccac gggagactcg
      361 aatccgtccg aggcgaacct cggctcgatg gcggtgctcg tagtcgacga ctccgcgccc
      421 ggcggcgcgg acctccaacg cgccgtcctg acggcgcgca cccaggaagt gtccgtgctc
      481 cacgagcgcg gccgctggat gtagcaccca cccgaagaga cctcatgaac tcagatcaga
      541 tgcacggcca ttggcgcgaa gtaaagggtt gggcgctcga gcagctcggg cgagtcacgc
      601 aggatccgtg gaagcggatc gccggccgtc gcgagcgcct gctcggccgc ctccaggcct
      661 ccctggccga atcgcgccac atcgccgagt cgagctttcc ggcgtcgcaa acgggcgcca
      721 tccttgtgga gcgccgcagg acaagggagc tctgacccgc tacgcggcga ggcgcgatcc
      781 gggcgaggcg cgcagcagct tgtcgaggag cgcggcgccg tcgaattcgt cggcgaggcg
      841 cgactgcggg ccggcgaaga agtatccctg aagatagtcg gccttggctt cgatcgcgag
      901 gagcgcggcg gcccgcgact cgatgccctc caccgcgacc ttgcattgga attcatgcag
      961 cagctcgatc attcccggga gcatgcgtcg cgccttttcg ttgccgacga cgccggcgag
     1021 gatcgagcgg tcgaccttca ccacgtcggg gcgcaatgcg acgatgcgat cgaagttcga
     1081 gcagccgacg ccgaagtcgt ccatcgcgat cgaagcaccc aggccgcggt agcactcgac
     1141 cgcctcgcgc agcagcttct cgctcgagca cggcgcctcg aggatctcga cgcatacgcg
     1201 cttgggaacg agcccgtagt agcggatgag ctcggcgaac tcgcggccgc ggcgcgcgtc
     1261 gcgcaccgcc gcttcggggt ggatgttgat gaagagcgtg cggtcgcccg gatcgaccgt
     1321 cgcgtagttg cgaagatgca gcgcgcggca ggtccagtcg aggagcgagc gagcgccgtc
     1381 gggcgcgcgc gcgaagagct cctcggggct caccgtcgtg ccggactcgt ccgatgcgcg
     1441 caccagcgcc tcgaagccga ggcaggcggc gcggcgcacg cagtagatcg gctggaaatg
     1501 ggtggtaagg ctcgcgccct cccattgcag ggacgagccg cccgcggcgc gcaccagttc
     1561 ctcggccgcg tgcgcgagcg agggcgtcgc gagctcgccc tcggccccgc ctcgaggcgg
     1621 cgtcgccatt ccctgtccat ccctcatgag gtgccccgcg ctccgtgatt gcacgccgga
     1681 cgtgcgccgg cttcatggca ctttggggcg agcgcgttac cgcggggtta cgcgcccccg
     1741 ccgggggatt gcgtttgtaa gaggccctgg gaggctccct aggccttcgc gaagaagatg
     1801 gcgagggcct cggcgatctc cctcatgcga tagggcttgc gcagcacgcg gttcaccccg
     1861 gcggaaagcg ctcgcgcctc gaagcccgcg ccgccgtatc cgctcgcgat cacgaccggc
     1921 agcgtgggat tgagcttgcg cagctccttg gcgagctgcg tgccgatcag ctccggcatg
     1981 acctcgtcgc tcaccaccgc atcgaagcgc cgtggatggg cgcgcatcgc ctcgagggcc
     2041 tcgcggctgc tgccgaagcc cacgggctcg tagccgagct gcgcgagcat ctcctccagc
     2101 gcgtgcagca cgtcgagctc gtcgtcgacc gcgaggatca cctggcccgc gcccatcggc
     2161 agcgtctcga gctcgtcggc ggcgccctcc tcggcgtgga tctgcgggat ccagacggtg
     2221 aacgtggtgc cgcgcccgag ctcgctggcg acctcgatga atcccttgtg ctccgagacc
     2281 accgagtgca cgagcgcgag gccaaggccc gtcccgcgcc ccgccggctt ggtcgtgaag
     2341 aacggctcga agatgcggtc gatcacctcc ggcgcgatgc cgtggcccgt atccttcacc
     2401 cagacgcgca cgtattcgcc cggcggaatc tcgccgcttc gcaccttgcg cggcgcggcg
     2461 accgtctcgc aggcgagccc gacctcgagc gtgccgccct gccccatgga atggatcgca
     2521 ttggtgcaga ggttcatgag gagctggtgc atgcgcgtgg gatcgccgag gatcgtcgcc
     2581 tcggcgcact ggtcggcgaa ggtcacgtcg atctccagcg gcgtcgagcc gcgaacgagg
     2641 tcgaccactt cctgcgcgat cggggccatg atcaccggga atttctccgc gccctccgcg
     2701 cggctgtagg agaggatctg ggtgacgagc gccttggcgc ggttgccggc ccccatgatg
     2761 gtgtcgaggt agcgcttcat gtcgccgtcg cccgcggcgc ggcgctgcgc gagctcgccg
     2821 tagccgagga tcgcgccgag gatgttgttg aagtcgtgcg cgatgccccc ggcaaggtgg
     2881 cccaccgcct gcatcttttc cgcttgcttg agctgcgctt cgaggcgcac gcgctcctcc
     2941 tcggcgtgca ccttgtcgga aacgtccttc acgaagccga tgacgcgccg cgtgccgggc
     3001 gcctccgcgt cgggatcgcc gatgcgatag gcgttcacgc cgagcgtcgt gtagacgccg
     3061 tcgtcgcggc ggaagcggta ctcgagcgcg acgtgcgaga actcgcgctg gcgcagccgc
     3121 tggcgcagcc ccttcagccg gatgcggtcg tcggggtgca cgcgctccat ccacttgcgc
     3181 aggtcggaga tggcgtcgtg gcccacgccg agcaccgatt gcgtgtcgcc gtcccagatc
     3241 accttgccgt cgtcgaggtt caactcgtac aggaggttgc cgctcgcgcg gatcacggcc
     3301 tcgtggcggg cgcgccaccc ctcggtctcg cgcgccgcgt cctggcgctc caccaggagc
     3361 gccgccgaga aaagcgtcgt caccacgatc gtggcgaggt agccctgcag gtgcagcatc
     3421 gagcgcgcgt cggcgaagcc gtcgaccgcg aacggcccga agccatgggc ggtctgccag
     3481 tagcagatga gcccgaagat cgacaggccc agcgtggcgg cgcgcagccc gaagcgcaac
     3541 gccgcccaaa tgaagaaggg ggcgcacagg tagatcaggg gcgggatgaa tccgggcacc
     3601 gtg
//
LOCUS       DASVII010000066         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_75339, whole
            genome shotgun sequence.
ACCESSION   DASVII010000066 DASVII010000000
VERSION     DASVII010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75339"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VF309_00845"
     CDS             complement(<1..324)
                     /locus_tag="VF309_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7465156.1"
                     /translation="MAEPDLTPRFEEVTALGPHGLTKISYAEWGPRDAAQVVLCVHGL
                     TRNKRDFDYLALRLAHRGLRVLAADLPGRGDSGWVSTPTDYATPLYLAAVAAVIARSG
                     AKSVDY"
     gene            complement(333..1430)
                     /gene="phaC"
                     /locus_tag="VF309_00850"
     CDS             complement(333..1430)
                     /gene="phaC"
                     /locus_tag="VF309_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEX7465157.1"
                     /translation="MRVKVDPQEAVKEFSDFSQRLQKGRDLLERTRGDDVQIATTPKT
                     EVFRQDKVVLYHYKPSAKKTIGVPVLVVYGLVGRYTMADLQEDRSLVRNLLAQGVDLY
                     VVDWGNPTRGDRWLTLEDYIDGYIGECVDFIRAKHGIDKVSLLGICEGGVFTLCYAAR
                     NPGRVKNLVLTITPVDFHSGSKDAPLHHGMIGLWARSMTGEDVDRLIDANGNLPGELM
                     SFVFSMMTPMNALTKYNLGFLEVMDDEKKLLNFLRMEKWLADRPDHPGEAAKQWLKEL
                     YQGNQLVKGEFKLAGEKVNLKRITMPVLNIFAQEDHIIPPKSSQALKKHVGTKDYTEL
                     QLPGGHVGVFVSGKSQGILGKNIYGWLKGHA"
     gene            complement(1432..2235)
                     /locus_tag="VF309_00855"
     CDS             complement(1432..2235)
                     /locus_tag="VF309_00855"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEX7465158.1"
                     /translation="MKGFEQQGNEWLKFWNENQKSLFQAWAEGRPSPFAMQGEPPPGT
                     EVMSDLLQRSMEEWSTLSKDAWKKNGGFDAEAMKKLFDPAEWKRAGSHFDMGLEKLTE
                     GPTYATLWDLDKKMLNTQKLWVERARDVEHYWEVIQGAWNRALERFMAAVNDPKGKPL
                     QSGRQMLDLWLETANKSLVEMHRSKEFLEAQRRMTRSSTEYRLAEREIAEAFCEMHHI
                     PTRTEMDEVQRTVTELRRELRTMKREGRALPKAAVAPSRAKAPAKRRRK"
     gene            complement(2246..>3198)
                     /locus_tag="VF309_00860"
     CDS             complement(2246..>3198)
                     /locus_tag="VF309_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7465159.1"
                     /translation="PRPTPMALGHEAAGTVEELGEGVSDLKKGDHVVLVFIPSCGHCN
                     PCAEGRPALCEPGAAANTAGTLLTGEGHLFRNDGSRVYHHMGCSAFAEYAVVSRHSAV
                     KVDPALPLDEAALFGCAVLTGVGAVVNTAQVRAGSSVAVVGLGGVGLASILGAVASGA
                     RAVVAVDLSAEKLALAKDLGATHTFNAAEPDLVDKVKAATKGGVDYSIEMAGSTRAFE
                     TAYRITRRGGTTVTAGLPPPAATWAMPSTNLVAEERTIKGSYIGTCVPSRDLPRYIEL
                     YQQGKLPVNRLMTGRLTLDQINEGFDLLHEGNAVRQVVTF"
BASE COUNT          470 a         1064 c         1069 g          595 t
ORIGIN      
        1 gtagtcgacg gatttggcgc cgcttcgcgc gatcaccgcc gccaccgccg cgaggtaaag
       61 cggcgtcgcg taatccgtcg gcgtcgatac ccatccgctg tcgccgcgcc cgggaaggtc
      121 ggcggcgagg acgcgcagcc cgcgatgggc gaggcgtagc gcgaggtaat cgaagtcgcg
      181 cttgttgcgc gtgaggccgt gcacgcaaag gacgacctgc gcagcgtcgc gaggtcccca
      241 ctcggcgtag gagatcttcg tcaggccgtg gggcccgagg gccgtgacct cttcgaagcg
      301 cggggtcaga tcgggctcgg ccatcgccgg ggtcaggcgt ggcccttgag ccagccgtag
      361 atgttcttgc cgaggatgcc ctgggacttg ccactcacga agacgccgac gtggccgccc
      421 ggcagctgca gctcggtgta gtccttcgtg ccgacgtgct tcttcagcgc ctgggagctc
      481 ttcggcggga tgatgtggtc ctcctgcgcg aagatgttca gcaccggcat cgtgattctc
      541 ttcaggttca ccttctcgcc cgcgagcttg aactcgccct tcacgagctg gttgccctgg
      601 tagagctcct tcagccattg cttggcggcc tcgcccgggt ggtcggggcg atcggcgagc
      661 cacttttcca tccgcaggaa attgaggagc ttcttctcgt cgtccatcac ctcgaggaag
      721 cccaggttgt acttggtgag ggcgttcatc ggggtcatca tcgagaagac gaagctcatg
      781 agctccccgg gcaggttgcc gttcgcgtcg atgaggcggt cgacgtcctc gcccgtcatg
      841 ctgcgcgccc ataggccgat catgccgtgg tgcagcggcg cgtccttcga cccggagtgg
      901 aagtcgacgg gcgtgatggt gaggacgagg ttcttcacgc ggccgggatt gcgcgcggca
      961 tagcagaggg tgaagacgcc gccctcgcag atcccgagga gcgagacctt gtcgatgccg
     1021 tgcttcgcgc gaatgaagtc gacgcactcg ccgatgtagc cgtcgatgta gtcctcgagg
     1081 gtgagccacc ggtcgccgcg cgtgggattg ccccagtcga ccacgtagag atcgacgccc
     1141 tgcgcgagaa ggttgcgcac gagggagcgg tcctcctgca ggtcggccat cgtgtagcgg
     1201 cccaccaatc cgtacacgac gaggaccggg acgccgatcg tcttcttcgc gctcggcttg
     1261 tagtgataca ggaccacctt gtcctggcgg aacacctcgg tcttcggcgt ggtcgcgatc
     1321 tgcacgtcgt cgccgcgcgt gcgctcgagg aggtcgcggc ccttctgcag gcgctggctg
     1381 aagtcggaga actccttgac ggcttcctgg gggtcgactt tcacgcgcat gtcatttcct
     1441 gcggcgcttc gccggtgcct tggccctgct cggtgccacc gcggccttcg gcaacgcgcg
     1501 cccttccctc ttcatcgtcc ttagctcgcg ccgcagctcc gtcaccgtgc gctgcacctc
     1561 atccatctcg gtacgcgtcg ggatatggtg catctcgcag aaggcttcgg cgatctcgcg
     1621 ctcggccagg cgatactcgg tcgaggagcg cgtcatgcgc cgttgcgcct cgaggaattc
     1681 cttggagcgg tgcatctcga cgagcgactt gttcgcggtt tccagccaca ggtcgagcat
     1741 ctggcggccg ctctggagtg gcttgccttt cgggtcgttg accgcggcca tgaagcgctc
     1801 gagggcgcgg ttccaggcgc cctggatcac ttcccagtaa tgctcgacgt cgcgcgcgcg
     1861 ctcgacccac agcttctgcg tgttgagcat cttcttgtcg aggtcccaga gggtcgcgta
     1921 cgtcggccct tcggtgagct tctcgaggcc catgtcgaag tgggagccgg cgcgcttcca
     1981 ctccgccgga tcgaagagct tcttcatggc ctcggcgtcg aagccgccgt tcttcttcca
     2041 cgcgtccttc gacaacgtgg accactcctc catggagcgc tgcaacaggt cgctcatcac
     2101 ctccgtgccc ggcggcggct cgccctgcat ggcgaaaggc gagggccggc cttccgccca
     2161 cgcctggaag aggctcttct ggttctcgtt ccagaacttg agccactcgt tgccctgctg
     2221 ctcgaatccc ttcatcgcat ttcgtctaga aggtcacgac ctggcgaacg gcgttgccct
     2281 cgtggagcag gtcgaagccc tcgttgatct ggtctagcgt gaggcgtccg gtcatgaggc
     2341 gattcacggg aagcttgccc tgttggtaga gctcgatgta gcgcggcagg tcgcgcgagg
     2401 gcacgcaggt gccgatgtag ctgcccttga tggtgcgctc ttcggcgacg aggttggtcg
     2461 agggcatggc ccacgtcgcg gcgggcggag gcaggcccgc ggtgaccgtg gtgccgccac
     2521 gccgcgtgat gcggtacgcc gtctcgaagg cgcgcgtcga gcccgccatc tcgatcgaat
     2581 agtcgacgcc gcccttggtc gccgccttca ccttgtcgac gaggtcgggc tccgccgcgt
     2641 tgaaagtatg ggtggcgccg aggtccttcg cgagcgcgag cttctccgcg gaaagatcga
     2701 cggccaccac cgcgcgcgcg cccgatgcga ccgcgccgag gatcgaggcg agcccgacgc
     2761 cgccgagccc gacgaccgcg accgacgatc cggcacggac ctgcgccgtg ttcacgaccg
     2821 cgcccacgcc cgtcagcacc gcgcacccga atagtgcagc ctcgtcgagc ggaagcgcgg
     2881 gatcgacctt caccgcggaa tggcgcgaga ccacggcgta ctccgcgaac gccgagcagc
     2941 ccatgtggtg atagacgcgc gacccgtcgt tgcggaaaag atgcccttcg cccgtgagca
     3001 acgtgcccgc ggtattcgcc gcggcgccgg gctcgcacag cgcgggccgt ccctcggcgc
     3061 acggattgca atggccacag ctcggaatga aaacgaggac gacgtgatcg cccttcttca
     3121 ggtcggagac gccctctccc agctcctcga cggttcccgc cgcttcatgt ccgagcgcca
     3181 tgggcgtggg ccggggcc
//
LOCUS       DASVII010000067         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_147350,
            whole genome shotgun sequence.
ACCESSION   DASVII010000067 DASVII010000000
VERSION     DASVII010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147350"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /gene="aspS"
                     /locus_tag="VF309_00865"
     CDS             complement(<1..1494)
                     /gene="aspS"
                     /locus_tag="VF309_00865"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX7465160.1"
                     /translation="MRTEYCGNTDTRFLGQSVTLFGWAHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRAETFAIAEGVRNEFVLRATGIVRRRPEGTVNAALRSGEIEVLAHELEIL
                     NPSATPPFMIDEEHVSEEVRLKYRYLDLRREPMQKALKLRHATSRAVRGYLDDAGFID
                     VETPVLYKSTPEGAREFLVPSRIHDGQFYALPQSPQLFKQLLMVSGFDRYYQIVKCFR
                     DEDLRAERQPEFTQIDVETSFLTEREIQDLMEGLVRHVFKRVLDVDVAPFPRLTYAEA
                     TSRYGSDKPDLRVKLELTDLTDVMKDASFKVFKSAAELKDGRVAALRIPGGNEQFTRK
                     ELDDLTPFVAIYGAKGLAYIRVNDPAKLNEEGLQSPIVKFLSPEILAKILERTGAKAG
                     DLIFFGADRRKIVNDALGALRMKVGHEKGHAEAGWRPVWVVDFPAYEYDEEGKRWSAA
                     HHPFTAPKDEHVPLMESDPGKVLAKAYDIALNGWELGGGSVRIHRSEV"
     gene            complement(1509..>1710)
                     /locus_tag="VF309_00870"
     CDS             complement(1509..>1710)
                     /locus_tag="VF309_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465161.1"
                     /translation="GPDQISVFVPTTPNITAGFFLIVPRSETVELEMTVDAALKYIIS
                     MGVAEPPARGSIEATRTAPPSA"
BASE COUNT          258 a          558 c          586 g          308 t
ORIGIN      
        1 cacctcggaa cggtggatgc gcaccgagcc gccgcccaat tcccacccgt tgagcgcgat
       61 gtcgtaggcc ttcgcaagga ccttgccggg atcggactcc atgagcggca cgtgctcgtc
      121 cttcggcgcc gtgaaggggt ggtgcgccgc gctccagcgc ttgccctcct cgtcgtactc
      181 gtacgcgggg aagtcgacca cccacacggg ccgccagccc gcttccgcgt ggcccttctc
      241 gtggccgacc ttcatgcgca aggcgccgag cgcgtcgttc acgatcttgc ggcggtccgc
      301 gccgaagaag atcaggtcgc ccgccttggc gccggtgcgc tcgaggatct tcgcgagaat
      361 ctcgggcgag aggaatttca cgatgggcga ctgcagcccc tcctcgttca gcttcgcggg
      421 gtcgttcacg cggatgtagg cgaggccctt cgcgccgtag atcgcgacga aaggcgtgag
      481 gtcgtcgagc tccttcctcg tgaactgctc gttgccgccg ggaatgcgca gcgcggcgac
      541 gcgcccgtcc ttcagctccg cggccgactt gaacaccttg aagctcgcgt ccttcatgac
      601 gtcggtgaga tcggtgagct cgagcttcac gcgcaggtcg ggcttgtcgc tgccgtacct
      661 cgaggtggcc tcggcgtagg tgaggcgcgg aaacggcgcg acgtcgacgt cgagcaccct
      721 cttgaagacg tggcgcacga ggccttccat caggtcctgg atctcgcgct cggtgaggaa
      781 ggaagtctcg acgtcgatct gcgtgaactc gggctggcgc tcggcgcgca ggtcctcgtc
      841 gcgaaagcac ttcacgatct ggtagtagcg atcgaagccc gagaccatca gcagctgctt
      901 gaagagctgc ggcgattgcg gcagcgcgta gaactgcccg tcgtgtatgc gcgagggcac
      961 gaggaactcg cgcgcgccct cgggcgtgga cttgtagagg accggcgtct cgacgtcgat
     1021 gaagcccgcg tcgtcgaggt agccgcgcac cgcgcgcgac gtcgcgtggc gcagcttcag
     1081 cgccttctgc atcggctcgc ggcgcaggtc gaggtagcgg tacttgaggc gcacctcctc
     1141 ggagacgtgc tcctcgtcga tcatgaaggg cggcgtggcc gagggattga ggatctcgag
     1201 ctcgtgggcg aggacttcga tctcgccgct cctcaaggct gcgttcacgg tgccctcggg
     1261 cctgcgccgc acgatgccgg tcgcgcgcag cacgaactcg ttgcgcacgc cctcggcgat
     1321 cgcgaaggtc tccgcgcgat cgggatcgca gaccacttgc accagccctt cgcggtcgcg
     1381 caggtcgatg aagatcacgc cgccatggtc gcggcggcgg tgcgcccatc cgaagagggt
     1441 gacgctctgg ccgaggaatc gggtgtctgt gttgccgcag tattcggtgc gcattctggt
     1501 tctcgtcgtc acgcggacgg tggcgctgtc ctcgttgctt caatcgaccc gcgcgccggc
     1561 ggttccgcga cgcccatcga gatgatgtac ttcaacgccg cgtccaccgt catttcgagc
     1621 tcgacggtct cggagcgcgg cacgatcagg aagaaacccg cggtgatgtt gggcgtggtc
     1681 ggaacgaaga ccgagatctg gtccggcccg
//
LOCUS       DASVII010000068         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_686861,
            whole genome shotgun sequence.
ACCESSION   DASVII010000068 DASVII010000000
VERSION     DASVII010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686861"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF309_00875"
     CDS             complement(<1..481)
                     /locus_tag="VF309_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7465162.1"
                     /translation="MSESLPFVSVVVPVLDEAEGIGRALAGLQALRDAGGEVIVVDGG
                     SRDATRAIASSLADRVIDAPRGRALQMNAGARAARGPLLVFLHADTVPPEGALEGLGA
                     RLEASGRSWGRFDVSIDAADPLLALVALLMNARSRWTGIATGDQAIFARRDAFERAGG
                     "
     gene            complement(510..1319)
                     /locus_tag="VF309_00880"
     CDS             complement(510..1319)
                     /locus_tag="VF309_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX7465163.1"
                     /translation="MKKFLLSIAAFLVSVNALAFDHGYRAWDELLKKHVKYVQDANAS
                     RVDYTGFAKDRAQLKAVLDDYSKVTRSEFDAWTKPQQQAFLINAYNAFTVELILTKYP
                     DLQSIRDLGSFVSKPWSKKFFRLFGQESYLDFIEHEMLRKEGVYDDPRVHVGVVCASI
                     GCPMLRNEAFVPEKLDSQLDDGMRRFLSDRTRNRYNPQSRKLEISKIFDWYGKDFEKG
                     HKGFASVKGVMARYADQLADKPEDRALVKAEKADITFLDYDWSLNDVKSSQ"
     gene            complement(1323..>1525)
                     /locus_tag="VF309_00885"
     CDS             complement(1323..>1525)
                     /locus_tag="VF309_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465164.1"
                     /translation="PTLPEANKFVAGVWKRKQVTRGQMAFVQSFNEWTRGEAGIGAVV
                     AKLFSLGDKRPYYEVAENHGDD"
BASE COUNT          254 a          500 c          497 g          274 t
ORIGIN      
        1 agccgccggc gcgttcgaag gcgtcgcgcc gcgcgaagat cgcctggtcg cccgtggcga
       61 tgccggtcca gcgcgagcgc gcgttcatga gaagcgccac cagcgcgagc aggggatcgg
      121 ctgcgtcgat ggagacgtcg aagcgtcccc aggaacgccc cgaggcttcg aggcgcgcgc
      181 cgaggccttc gagcgcgccc tcgggcggca ccgtgtcggc atgaaggaag acgaggagcg
      241 ggccgcgcgc ggcccgggcg cccgcgttca tctgcagcgc gcgcccccgg ggggcgtcga
      301 tcacgcggtc ggccagcgac gaggcgatcg cgcgagtcgc gtcgcggctg ccgccgtcga
      361 cgacgatcac ctcgccgccc gcgtcgcgca gcgcctgcag ccccgccagg gcccggccga
      421 tgccttcggc ctcgtcgagg accggaacca ccaccgaaac gaaggggagg gactcgctca
      481 tcccccaaga ataatggagt ccggcccttt cattgtgaag acttcacgtc gttgagcgac
      541 cagtcgtagt cgaggaacgt gatgtcggcc ttctcggcct tcaccaacgc gcggtcctcg
      601 ggcttgtcgg cgagctggtc cgcgtagcgc gccatgacgc ctttcaccga ggcaaagccc
      661 ttgtggccct tctcgaaatc cttgccgtac cagtcgaaga tcttcgagat ctcgagcttg
      721 cggctctggg ggttgtagcg gttgcgggtg cggtcggaaa ggaagcgcct catgccgtcg
      781 tcgagctgcg agtcgagctt ctcggggacg aacgcttcgt tcctcagcat cgggcaaccg
      841 atcgacgcgc acaccacgcc cacgtggacc cggggatcgt cgtagacgcc ttccttgcga
      901 agcatctcgt gctcgatgaa gtcgaggtac gactcctggc cgaagagccg gaagaacttc
      961 ttgctccacg gcttgctcac gaaactgccg agatcgcgaa tcgactggag gtcggggtac
     1021 ttggtgagga tcaattccac cgtgaacgcg ttgtacgcat tgatgaggaa ggcctgctgc
     1081 tgcggcttgg tccacgcgtc gaactcgctc ctcgtgacct tcgaatagtc gtcgaggacc
     1141 gccttcagct gagcgcggtc cttcgcgaag cccgtgtagt cgacgcgcga ggcgtttgcg
     1201 tcctggacgt atttcacgtg cttcttcagg agctcgtccc acgccctgta gccatggtcg
     1261 aacgcgagcg cgttcacgct caccaggaat gccgcgatgg aaaggaggaa tttcttcatg
     1321 gctcagtcgt ctccgtgatt ttccgcaact tcatagtagg gacgcttgtc gccgagggaa
     1381 aagagcttcg ccaccacggc gccgatcccg gcctcgccgc gcgtccattc gttgaaggac
     1441 tggacgaacg ccatctgccc gcgggtcacc tgtttcctct tccagacgcc cgccacgaat
     1501 ttgttcgcct ccggcagcgt gggat
//
LOCUS       DASVII010000069         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_363339,
            whole genome shotgun sequence.
ACCESSION   DASVII010000069 DASVII010000000
VERSION     DASVII010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363339"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VF309_00890"
     CDS             complement(<1..254)
                     /locus_tag="VF309_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465165.1"
                     /translation="MNHRIDRRRFVLSVASSLAAATLPGAVRAAMAPNDKFDLVIKGG
                     EVLDPSQRLRAVRDIGIKNGIVEAVEVGIPAERALRTIDAT"
     gene            364..>1502
                     /locus_tag="VF309_00895"
     CDS             364..>1502
                     /locus_tag="VF309_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7465166.1"
                     /translation="MKSTTKNRKSAPRAADSRSGDLFADSAGQPPREPPVPPSRGGEP
                     PQRAAEGPSLSEFASRAYLAYAMSVVKGRALPSVEDGQKPVQRRILFTMREMGNRSDS
                     PHKKSARVVGDVIGKYHPHGDVAVYEAAVRMAQDFTLRYPLIDGQGNFGTRDGDSPAA
                     YRYTEVRLTDFAEQVLLSELDRGTVDFIDNYNGEFQEPKLLPARLPVMLLNGASGIAV
                     GMATEIPPHNLKEVAEACAQTLENEPDAKPVIRPIKGPDFPGGGQIISSRDEIKRAYE
                     TGRGSVRVRARWKVENLARGQYRVAVTELPPNTSAARVLLEIDELTNPKPKTGKKSLT
                     PDQQNLKAAALALLESQRDDSDGEHPVRLVFEPRTSKVDSEELMA"
BASE COUNT          262 a          515 c          503 g          222 t
ORIGIN      
        1 gtcgcgtcga tggtgcgcag cgcgcgctcc gcgggaattc ccacctcgac agcctcgacg
       61 atcccgttct tgatgccgat gtcgcgcacc gcgcgcaggc gctggctcgg atccaggacc
      121 tcgccgccct tgatcacgag atcgaacttg tcgttgggag ccatcgcggc gcgcacggcg
      181 ccgggcagcg tcgcggcggc gagcgaggaa gcgacggaga ggacgaagcg gcggcggtcg
      241 atccggtggt tcatggtgtt tcctccgggg aagttttgtt tttttcgcgc cggccgatgg
      301 tgaaccggcg accgcgcgag gtcaaggacg ttagaatcgg catctttgga tcgccgctgc
      361 gccatgaaat ccaccaccaa gaaccggaag tccgcgccac gcgcggcgga cagccgttcc
      421 ggcgacttgt tcgcggactc cgcggggcag cctccacgcg agcctcccgt tccgccgtcg
      481 cgcggcggcg agccgccgca gcgcgcagcc gagggccctt cgctatcgga attcgcttcg
      541 cgcgcctatc tcgcctatgc gatgagcgtg gtcaagggtc gcgcccttcc ctccgtcgag
      601 gacggccaga agccggtgca gcgacgcatc ctcttcacca tgcgcgagat gggcaaccgc
      661 tccgactcgc cgcacaagaa gagcgcgcgc gtcgtgggcg acgtgatcgg caaataccat
      721 ccgcacgggg acgtggcggt gtacgaggcc gcggtgcgca tggcgcagga cttcacgctg
      781 cgctatccgt tgatcgacgg ccaaggcaac ttcggcacgc gcgacggcga cagccccgcg
      841 gcctaccgct ataccgaggt gcgcctcacc gacttcgcgg agcaggtgct gctctcggag
      901 ctcgaccgcg gcaccgtcga cttcatcgac aactacaacg gcgaattcca ggagccgaag
      961 ctgctgcccg cgcgccttcc ggtgatgttg ctgaacggcg cctccggcat cgcggtcggc
     1021 atggcaaccg agatcccgcc gcacaacttg aaggaagtcg cggaagcctg cgcgcagacg
     1081 ctcgagaacg agcccgatgc gaagccggtg atccgcccca tcaagggacc ggatttcccg
     1141 ggcggcgggc agatcatctc gtcgcgcgac gagatcaagc gcgcctacga gaccggccgc
     1201 ggcagcgtcc gcgtgcgcgc gcgctggaaa gtggagaacc tcgcgcgcgg ccagtaccgc
     1261 gtcgccgtca ccgagctgcc gccgaatacg tccgccgcga gagtcctgct ggagatcgat
     1321 gagctcacca atcccaagcc gaagacgggc aagaaatcgc tcacgcccga ccagcagaac
     1381 ctgaaggcgg cggcactcgc gctgctcgag agccaacgcg acgattccga cggcgagcat
     1441 ccggtgcgcc tggtgttcga gccgcgcacc agcaaggtcg attccgagga gctcatggcg
     1501 tt
//
LOCUS       DASVII010000070         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_614750,
            whole genome shotgun sequence.
ACCESSION   DASVII010000070 DASVII010000000
VERSION     DASVII010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614750"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VF309_00900"
     CDS             <1..1035
                     /locus_tag="VF309_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HEX7465167.1"
                     /translation="GQVVMLTGAGGSIGSELCRQIAAFQPSMLVLFEMNEYAIYRVDE
                     QLRELFPEIPVASVVGDIKNARRVNQVMHQYSPALVFHAAAYKHVPLMEEANAWEAVR
                     NNVLGTYVVARAAIDCGVKKFVFISTDKAVNPTNVMGATKRLAEMVCQALQADAGNST
                     RFEMVRFGNVLGSAGSVIPKFQEQIDRGGPVTVTHPEMVRYFMSIPEAAQLVLQAGCM
                     GLGGEIFVMDMGEPVKIVDLARDMIRLSELSENEIRIVYTGLRPGEKLFEELLADDEH
                     TRPTPHPKLRIAKAREVNTGWIDGLLDWLNQDRIPGDSEVRRDLKRWVPEFQSQVRPR
                     LTSIPGAKAL"
     gene            complement(1032..>1916)
                     /locus_tag="VF309_00905"
     CDS             complement(1032..>1916)
                     /locus_tag="VF309_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7465168.1"
                     /translation="LHLWLRMLLVATIVLGVGEWLRLLLPGQGMISKASMGPGPFSTH
                     VVLMAPLLLFALWPGPIGMGRSLRLTLLLGALLVIAGIAGDSRILWPALLVSALVAFV
                     AFSAQTSPQHPSRKAAKRAFIVAIVLLPLLMLVSTDYKLRKYPQAKTTVESFAFDERP
                     LIWKTAVRAVAQRPWIGHGYGREIIAADLRAELAKAGQLKPYNHGHNVLLDAAIQMGA
                     LGLAVFAAMMGALAWAFAAARKREAGAVLAIVGLAMFAGYFTKNLTDDFFFRPNSLVF
                     WAIAGMLLGLASRLPPRA"
BASE COUNT          354 a          618 c          658 g          286 t
ORIGIN      
        1 gggcaggtcg tgatgctgac gggcgcgggc ggctcgatcg gctccgagct ttgccgccag
       61 atcgccgcgt tccagccgtc gatgctggtg ctcttcgaga tgaacgagta cgcgatatac
      121 cgggtcgacg agcagctgcg cgagctcttc cccgagattc ccgtggcgag cgtggtgggc
      181 gacatcaaga acgcgcgccg cgtgaaccag gtgatgcacc agtactcgcc ggccctcgtg
      241 ttccatgccg cggcgtacaa gcacgtgccg ttgatggaag aggccaacgc gtgggaagcg
      301 gtgcgcaaca acgtgctcgg cacctacgtc gtggcgcgcg cggcgatcga ttgcggagtg
      361 aagaaattcg tcttcatctc gaccgacaag gcggtgaatc ccaccaacgt gatgggcgcg
      421 accaagcgcc tcgccgagat ggtgtgccag gccctgcagg ccgacgccgg aaacagcacg
      481 cgattcgaga tggtgcgttt cggcaacgtg ctcggcagcg ccggcagcgt gatcccgaag
      541 ttccaggagc agatcgacag gggcggcccg gtgaccgtca cccacccgga gatggtgcgt
      601 tacttcatgt cgatccccga ggcggcgcag ctcgtgctgc aggccggctg catgggcctc
      661 ggcggcgaga tcttcgtgat ggacatgggc gagcccgtga agatcgtcga tctcgcgcgc
      721 gacatgatcc ggctgtcgga gctttccgag aacgagatcc gcatcgtcta cacgggcctg
      781 cgccccggag agaagctctt cgaggagctc ctcgccgacg acgagcacac gcggcccacg
      841 ccccatccga agcttcgcat cgcgaaggcg cgcgaggtga atacgggatg gatcgatgga
      901 ttgctcgact ggctcaacca ggaccgcatc ccgggcgatt ccgaggtgcg ccgcgacctc
      961 aagcgctggg tgccggaatt ccagtcccag gtgcgcccgc gccttacctc gattccgggc
     1021 gcgaaggcgc tctaggcccg cggcggaagg cgcgacgcga gccccagcag cattcccgcg
     1081 atcgcccaga agaccaggga attggggcga aagaagaaat cgtcggtgag gttcttcgtg
     1141 aagtagccgg cgaacatcgc gaggcccacg atcgcgagca ccgctcctgc ctcgcgcttg
     1201 cgcgccgcgg cgaaggccca cgccaacgcg cccatcatcg ccgcgaagac tgcgaggccc
     1261 aatgccccca tctggatcgc ggcgtcgagc agcacgttgt ggccgtggtt gtagggcttg
     1321 agctgcccgg ctttcgcgag ctcggcacgc aggtccgcgg cgatgatctc gcggccatac
     1381 ccgtgaccga tccaggggcg ttgcgcgacc gcgcgcacgg cggtcttcca gatgaggggg
     1441 cgctcgtcga acgcgaacga ctcgacggtg gtcttggctt gcggatattt gcgcagcttg
     1501 tagtcggtcg acaccagcat cagtaacggc agcagcacga tcgcgacgat gaaggcccgt
     1561 ttcgcggcct tgcggctcgg atgttgcggc gaggtctgcg cgctgaaagc cacgaacgcg
     1621 accagcgccg agacgagaag agccggccac aggatgcggc tgtcgcccgc gattcccgcg
     1681 atcacgagga gggcgccgag gagaagcgtg aggcgaaggc tgcgtcccat gccgatgggc
     1741 ccgggccaca gcgcgaacag caggagcggc gccatcagca ccacgtgagt cgagaaagga
     1801 cccgggccca tgctcgcctt ggaaatcatt ccctgcccgg gcaggagaag cctcagccac
     1861 tcgcccacgc cgagcacgat cgtcgcgacg agcagcatcc gcagccacag gtgaag
//
LOCUS       DASVII010000071         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_75555, whole
            genome shotgun sequence.
ACCESSION   DASVII010000071 DASVII010000000
VERSION     DASVII010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75555"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF309_00910"
     CDS             <1..568
                     /locus_tag="VF309_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7465169.1"
                     /translation="SEFYQKQSGTRLRFMKEVLGGTWDALASGRADLAIGAGGDAPAG
                     RSYATKPLGRVEMVFVAAPFHAICNEPQPLTAEIIQRYRAVSVADSSRLLAPRTVGLL
                     SGQDVLTVPSMDAKAAAHIAGLGVGFLPRWIAEREALAGTLRVLAVDAPRPIAELLVA
                     WRPGQEGKALKWFVKRLEDPLVAAGLLS"
     gene            565..1476
                     /gene="gluQRS"
                     /locus_tag="VF309_00915"
     CDS             565..1476
                     /gene="gluQRS"
                     /locus_tag="VF309_00915"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX7465170.1"
                     /translation="MSAYRGRFAPSPTGPLHFGSLIAALASFLDARAAGGEWIVRIED
                     VDESRTVPGAGDAILRQLEAFGFEWDGAVAWQSRRTHLYEAALARLRAQGLIYRCRCS
                     RREIADSALHGVEGAIYPGTCRALGVDPSTPGADRLLVAGATLAFDDRVQGRISQDVT
                     REIGDFVLKRRDALHAYQLAVVVDDADQGITDVVRGADLLHSTPRQVLLQRLLGFATP
                     RYLHFPVATHESGEKLSKQTLAAAADDREAVALLGAGLRFLGQEAPDAAAPREILRHA
                     IAGWSPDAIPRRTELAVGAASAATPGV"
BASE COUNT          220 a          583 c          564 g          243 t
ORIGIN      
        1 ctccgagttt taccagaagc aatcgggcac gcggcttcgc ttcatgaagg aagtgctggg
       61 cggcacgtgg gacgcgcttg cctcgggccg cgcggacctc gcgatcggcg ccggcggcga
      121 cgctccggcg ggacgcagct acgcgacgaa gcccctgggg cgcgtcgaga tggtgttcgt
      181 cgcggcgccg ttccacgcga tctgcaacga gccccagccc ctcaccgcgg agatcatcca
      241 gcgttatcgc gcggtgagcg tcgcggattc gtcgcgcctg cttgcgccgc gaacggtggg
      301 gctgctctcg ggccaggacg tgctcacggt tccatcgatg gacgcgaaag ctgcggccca
      361 catcgcggga ctcggcgtgg gattcctgcc ccgctggatc gccgagcgcg aggcgctcgc
      421 cgggaccctg cgcgtcctcg cggtcgatgc gccgcgcccc atcgcggagc tcctcgtcgc
      481 gtggcgtccc ggccaggaag gaaaggctct caagtggttc gtgaagcgcc tcgaggatcc
      541 gctggtggcc gcggggctcc tgtcgtgagc gcctatcggg gccgcttcgc gccctcgccg
      601 acggggccgc tgcacttcgg ctcgctcatc gcagcgctcg cgagcttcct cgacgcgcgc
      661 gcggcgggcg gggaatggat cgttcgcatc gaggacgtcg acgaatcgcg cacggttccg
      721 ggcgcgggcg acgcgatcct tcgccagctc gaggccttcg gcttcgaatg ggacggcgcc
      781 gtcgcctggc agagccgccg cacgcatcta tacgaggcgg cgctcgcgcg cttgcgcgcc
      841 cagggactca tctaccgctg ccgctgctcg cggcgcgaga tcgcggactc ggcgctgcac
      901 ggagtcgaag gcgcgatcta tccgggcacc tgtcgcgccc ttggagtcga tccctcgacc
      961 ccgggcgccg accggctcct cgtcgccggc gcgacgcttg cgttcgacga tcgcgtgcaa
     1021 gggcgcatct cgcaggacgt cacgcgggag atcggcgact tcgtgctgaa gcgccgcgat
     1081 gcgctccacg cataccagct cgccgtggtg gtcgacgacg cggaccaggg catcaccgac
     1141 gtcgttcgcg gcgcggacct gctgcactcc acgccgcggc aggtcctgct gcagcgcctg
     1201 ctgggatttg ccacgcctcg ctacctgcat ttccccgtcg cgacccacga gagcggcgag
     1261 aagctctcca agcagacgct cgccgccgcg gccgacgatc gcgaggcggt ggcgctgctc
     1321 ggcgcggggc tgcgcttcct cggccaggaa gcgccggacg cggcggcgcc gcgggaaatc
     1381 ctccggcacg ccatcgcggg gtggtcgccg gatgccatcc cgcgccgcac cgagctcgcg
     1441 gtcggcgcgg cttcggccgc aaccccgggc gtataattcg acgacgctgc gtcgacgaat
     1501 ccaattgcgc gtactccggc atccaaccgg gtaccagcgc gttccccacc cgggaaactc
     1561 ggcgctgcac cgaaggctgc gaccatgctc ttcagaagga aagactccgc
//
LOCUS       DASVII010000072         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_686981,
            whole genome shotgun sequence.
ACCESSION   DASVII010000072 DASVII010000000
VERSION     DASVII010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686981"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF309_00920"
     CDS             complement(<1..190)
                     /locus_tag="VF309_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465171.1"
                     /translation="MVRPLLGHVGIHVKSSLARAWAWLVAFVRQLDASRTTGMAAELA
                     FWLFLALIPLAAVAGLVVA"
     gene            359..2095
                     /gene="malQ"
                     /locus_tag="VF309_00925"
     CDS             359..2095
                     /gene="malQ"
                     /locus_tag="VF309_00925"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7465172.1"
                     /translation="MQRPRSSGPDYVDVWVIDQGETRPIPEPSLLRLEDGTTRPSGSY
                     LARDLPLGYHELESVKSGERTRLIITPGRCYLPADLHTWGWAVQLYAVRSKKSWGMGD
                     LGDLRALARWSARALGAGMLLLNPLHAPMPTLPQHASPYYPSSRLFRNPLYLRIEDVP
                     GASASREALAPLAAAGALLSDAPRIDRNAVFALKLQALEKLFTRFRGSPSFDAYRAGE
                     GPLLGRYATFCALAERYGNHWNKWPRGYRHPDRPELGRFRNANRKRILFHEWLQWLID
                     AQLGRAGKEVPLVGDLAVGVDPGGADGWLWQDLFAQGMEVGAPPDEFNTQGQKWGLSP
                     LDPGMLRSSGYEPFVRIVRSAMRHMGGVRLDHVMGLFRLFWIPRGKKPKEGVYVRYPA
                     LDLLGIAALESVRAKAYFIGEDLGTVEAEVRRELAYRKTLSYRVLWFESGRPESYPKQ
                     AVSTVTTHDLPTIAGLWSGSDLATQRRLNLAPNEKGTRALRRSLAKLIRARKNAPIDE
                     VVMKTHAALARAPSALVTATLDDALSVEDRPNMPGTIDEYPCWCLPLPRKLEEIMKDP
                     RPRRIAKVLRER"
BASE COUNT          376 a          763 c          719 g          355 t
ORIGIN      
        1 tcgccacgac gaggcccgcg accgccgcca gggggatgag cgcaagaaag agccaaaagg
       61 ccagctcggc ggccatgccg gtggtccgcg aggcatcgag ctggcggacg aaagccacga
      121 gccatgccca agcgcgcgcg aggctcgact tcacgtggat gcccacgtgc ccgagcaacg
      181 ggcgtaccat gcggcgggct tggatgtggt gcctacgacc gggatgaaca tcggacctcg
      241 atagcgacac atgaatagcg tgaattggcg aagatggggg atcgaaccgg gctaccgaac
      301 ggcacagggc cactggcacc gcgcgcccga agcttcgctg cgcgtcgtgc gaggctcgat
      361 gcagagaccg cgttcgagcg gtcctgacta cgtcgacgtc tgggtcatcg accagggcga
      421 gacgcgaccc atccccgagc cgtcgctgct tcgcctcgag gacggcacca cgcgcccctc
      481 gggaagctac ctcgcacgcg acttgccgct cggctaccac gagctcgaaa gcgtgaagag
      541 cggcgagcga acgcgtctca tcatcacgcc ggggcgatgt taccttcccg ccgatctgca
      601 cacctgggga tgggcagttc agctctacgc cgtgcgttcc aagaagagct ggggcatggg
      661 cgatctcggc gatctgcgcg cgctcgcccg ttggtcggcg cgcgcgctcg gcgccggaat
      721 gctccttttg aacccgctgc acgctccgat gcccacgttg ccccagcacg cgagccccta
      781 ctacccgagc agccggctct ttcgcaatcc gctgtacctg cgcatcgaag atgttcccgg
      841 tgcctccgcc tcgagagaag ccctcgcccc cctcgccgcg gccggcgcgt tgctctcgga
      901 cgctccgcgc atcgatcgca atgcggtctt cgctctcaag ctgcaggccc tcgagaaact
      961 cttcacgcgc tttcgagggt caccatcgtt cgatgcgtac cgcgccggcg aaggtccgct
     1021 cctcggacgg tatgccacgt tctgcgcgct cgccgaacgc tacggcaacc actggaataa
     1081 gtggcctcgc ggctaccgtc accccgatcg ccccgagctc gggcgcttcc gcaatgcgaa
     1141 tcggaagcgc atcctgtttc acgagtggct gcagtggctc atcgacgcgc agctcgggcg
     1201 cgcgggcaaa gaggtgccgc tggtgggcga tctcgcggtc ggcgtcgatc ccggcggggc
     1261 cgatgggtgg ctctggcagg atctgttcgc gcaagggatg gaagtgggtg cgccgcccga
     1321 tgagttcaac acccagggcc agaagtgggg cctttcgccg ctcgatccgg gcatgctccg
     1381 ctcctcgggc tacgagcctt tcgtgcgcat cgtccggtcg gcgatgcggc acatgggcgg
     1441 cgttcgcctc gatcacgtga tgggtctctt tcggctcttc tggatcccgc gcggcaagaa
     1501 gccgaaagaa ggcgtctacg tccgctaccc ggcgctcgat ctgctgggga ttgcggctct
     1561 cgagagcgtc cgtgcgaagg cctacttcat cggcgaggac ctcggcaccg tcgaagccga
     1621 ggtgcgccgc gagctcgcgt acagaaagac gttgtcgtac cgcgtgctgt ggttcgagtc
     1681 gggccgtccc gagagctacc cgaagcaagc cgtcagcacc gtgaccaccc acgatcttcc
     1741 caccatcgcg ggactctggt cgggctcgga tctcgccacc cagcgcaggc tgaatctcgc
     1801 gcccaacgag aaaggcacgc gcgcgttgcg ccgctcgctc gccaagctca tccgcgccag
     1861 aaaaaatgcc cccatcgacg aagtcgtcat gaaaacgcac gccgccctcg cgcgcgctcc
     1921 gtccgctctc gtcacggcaa cgctggacga cgcgctctcc gtcgaggatc gccccaacat
     1981 gccgggcacg atcgacgaat acccttgctg gtgcttgccg ctcccacgaa agctcgaaga
     2041 gatcatgaaa gatccgcggc cccgccgcat cgccaaggtg ttgcgcgagc gctgaagcct
     2101 aggcctcgtc gcgcatgcca ccgaaggcgc ggtaggcgcg ggcgatgccg ctggcgacgg
     2161 tggctcgtcc gcgaggcgac tgtgcgtgga cgttttccag gcgaccagga gag
//
LOCUS       DASVII010000073         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_327432,
            whole genome shotgun sequence.
ACCESSION   DASVII010000073 DASVII010000000
VERSION     DASVII010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327432"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF309_00930"
     CDS             complement(<1..260)
                     /locus_tag="VF309_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465173.1"
                     /translation="MSRSCGLLVFLCFVAGSANEALAQSPFAGTWSGTNTITGSQTCT
                     FPPAGSIMTSCTGSAPWTGTIDSQGNFTLTLGTETITCDGIPT"
     gene            complement(318..1478)
                     /locus_tag="VF309_00935"
     CDS             complement(318..1478)
                     /locus_tag="VF309_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7465174.1"
                     /translation="MIHNRIAVALLIAGIAGFASMKVQAQGERTLDEVKAEAQARADR
                     NGYPLIGLKPEEVREALGRLHSLDRDEWAASWSEIGDRYMAKKDYEGAWRYYSFARWP
                     QPNSPGKQRAYEKALDAYLARAKSFDPPMEVIRVPYEGGEVVGYLRLPKSPRPAPLVV
                     AISGLDSRKEEMVERFAPLVERGVGVLALDSPGTGQSGVKAAPGAEKSLSRMIDAALA
                     RPEVDAKRVAVYGGSFGGHWATVLAVTEKARLRAVVAQSPLVHEAFLRERREALENNR
                     EYLFEYVPAWMFAYGVGNMDQLLAARERMSLKTRGMLDQPMAPMLVIGGALDTQVPIG
                     DIDLLLRSGDAPKDLWVHPRGGHMGRDAKAWPDPVIFRRVTAPWLLKMLEVQ"
     gene            complement(1507..>1725)
                     /locus_tag="VF309_00940"
     CDS             complement(1507..>1725)
                     /locus_tag="VF309_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465175.1"
                     /translation="PKRGVVLLSGDMVHLVYSWEHGIVPAFNFDVEQSRRSIAAMKEY
                     VAKSGAQVWINHDKAQHAAIPEAPAYVE"
BASE COUNT          265 a          594 c          565 g          301 t
ORIGIN      
        1 gtcggaatgc catcgcaggt aatcgtctcg gtcccaaggg tcaaggtgaa gttgccctgg
       61 gagtcgatcg tgcccgtcca gggcgcggac cccgtgcagg acgtcatgat ggagccggcc
      121 ggtgggaacg tgcaggtttg cgagccggtg atggtgttcg tgccgctcca cgtgccggcg
      181 aacgggctct gggctagcgc ctcgttcgcg cttcccgcga cgaagcaaag gaagacaagc
      241 aatccgcatg acctggacat atcgatcctc cgccgcgaga ttgaaggcca gcagtgcgag
      301 cagctacgag cgccgcgcta ttgtacctcc agcattttca gcagccacgg cgccgtcacg
      361 cggcgaaaga tcaccggatc gggccacgcc ttcgcgtcgc gccccatgtg gccgccgcgc
      421 ggatgcaccc agaggtcctt cggcgcgtcg ccgcttcgca gcagcaggtc gatgtcgccg
      481 atcggcacct gggtgtcgag cgcgccgccg atcacgagca tcggcgccat gggctggtcg
      541 agcatcccgc gcgtcttgag cgacatgcgt tcgcgcgccg cgagcagctg gtccatgttg
      601 ccgacgccat aagcgaacat ccacgccggg acgtattcga agagatattc gcggttgttc
      661 tcgagcgcct cgcgccgctc gcgcaggaac gcctcgtgca ccagcggcga ttgcgccacc
      721 acggcgcgca gccgggcctt ctccgtcacg gcgagcaccg tcgcccagtg gccgccgaag
      781 ctgccgccat agacggcgac gcgtttcgca tcgacctcgg ggcgcgccag ggccgcgtcg
      841 atcatgcgcg agagcgactt ctccgcgccg ggcgccgcct tcacgccaga ctgcccggtg
      901 ccgggagaat cgagcgcgag gactccgacg ccgcgctcga cgagcggcgc gaagcgttcc
      961 accatctcct ccttgcggct gtcgaggccg gaaatggcca cgacgagcgg cgcgggccgc
     1021 ggcgacttcg gcaagcgcag gtatccgacg acttcgccgc cctcgtacgg cacgcggatc
     1081 acttccatcg gcggatcgaa gctcttcgcg cgggcgaggt acgcatcgag cgccttttcg
     1141 taggcgcgct gctttcccgg cgagttcggt tgaggccagc gggcgaacga gtagtaacgc
     1201 cacgccccct cgtagtcctt cttcgccatg tagcggtcgc cgatctcgct ccatgaggcg
     1261 gcccactcgt cgcggtcgag gctgtggagg cgcccgaggg cttcgcgcac ttcctcgggc
     1321 ttcaggccga tcagcggata gccgttgcga tccgcgcgcg cctgggcttc ggccttcacc
     1381 tcgtcgagcg tgcgctctcc ctgcgcctgg actttcatcg aggcgaatcc cgcgatgccc
     1441 gcgatgagca gcgctacggc gattctgttg tggatcatga cggcgttcta cttcaatgcg
     1501 gttgcactat tcgacatacg ccggcgcctc gggaatcgcg gcgtgctgcg ccttgtcgtg
     1561 gttgatccag acctgcgcgc cgctcttcgc gacgtattcc ttcatcgcgg cgatgctgcg
     1621 cctgctctgc tccacgtcga aattgaatgc gggcacgatg ccgtgctccc agctgtacac
     1681 gagatgcacc atgtcgcccg agagcagcac gacgccccgc ttcgg
//
LOCUS       DASVII010000074         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_687063,
            whole genome shotgun sequence.
ACCESSION   DASVII010000074 DASVII010000000
VERSION     DASVII010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687063"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VF309_00945"
     CDS             complement(<1..370)
                     /locus_tag="VF309_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465176.1"
                     /translation="MRAIKSLLVAALAVAGMVATVSAQAQSHGGGSHASGGHSGGSHW
                     SGGGHGYYGGHGYYGGRYFYGPSVGFYLGAPLLFSPWYWGYPYDYYYPRTVVIDRVVD
                     DPAYYPEGMMQAPPPATTEVP"
     gene            complement(531..>1819)
                     /locus_tag="VF309_00950"
     CDS             complement(531..>1819)
                     /locus_tag="VF309_00950"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7465177.1"
                     /translation="IVLVAALILSQAASLWILHEHVTQPRMALGIGQFVSHLKTISAA
                     LQTMTPPQQQEFIGHIAEKDGIRISPVRGTERMRPAADVPAIELFRDRIRELFGPQAD
                     VYVRGAAAQTGEMPPQVLWVRLPAGERDFWIAFPRGRIERGPATALISWGIAGLLIAI
                     LATFFIVWKLTRPLSELARAAETFGKGVDPPPVEESGPSEIRGVARAFNQMMQGLQKS
                     QRDRATFLAGVSHDLRTPLSRLRLEVEMLDEKVDPGTRRAMVEDVGEMNAIIDQFIDF
                     MRSEADEPLTPVNLAELARSCAERAARTGVQVRCELSDVPTLMLRPLAMQRLVDNLLG
                     NAARHAGGEILVRTEHAGGMAVLSLLDRGPGIPENMVERLKEPFTRRDEARSGSSGAG
                     LGLAIANRVAALHGGKLELLPREGGGLEARLCLPAA"
BASE COUNT          302 a          606 c          625 g          286 t
ORIGIN      
        1 gcggcacttc ggtggtggcg ggcggcggcg cttgcatcat gccttccgga tagtaggcgg
       61 gatcgtcgac cacccgatcg atcacgaccg tgcgcggata gtaatagtcg tacggatagc
      121 cccaatacca gggactgaac agcagcggag cgccgagata gaagcccacg ctcggaccat
      181 agaagtatct gccgccatag tatccatggc cgccataata tccatggccg ccgccactcc
      241 aatggctgcc gccggaatgc ccgccgctgg catgggatcc gcccccatgc gactgggcct
      301 gggcactgac cgtggctacc ataccggcga cggcaagtgc cgccaccaac agcgatttga
      361 tcgctctcat ctctaggctc cttactttcg gaccccgatc tttgcaggat agcgcatggg
      421 cgcagcgcca gagtcaagga cggcgcccat tcggacaacg cttctaccca cacgttggat
      481 gacaggaagg acaagaaatt caccgggcgg cgcccacctt gaggggccca tcatgccgcg
      541 ggcaggcaga ggcgtgcctc gaggccccct ccctcgcgcg gcaggagctc cagcttgcct
      601 ccgtgcaggg ccgccacccg gttggcgatc gcgagcccca gcccggcccc ggagctgccg
      661 ctgcgagcct cgtcgcggcg ggtaaagggc tccttgagcc tctcgaccat gttctcggga
      721 attcccgggc cgcgatcgag cagggaaagg accgccatgc cgcctgcgtg ctccgtgcga
      781 acgaggatct cccctccggc gtgacgcgcg gcgtttccga gcaggttgtc cacgaggcgc
      841 tgcatcgcga gggggcgaag catgagcgtc gggacgtccg agagctcgca gcgcacctgc
      901 acgccggtgc gcgctgcgcg ttcggcgcag ctgcgcgcga gctccgcgag gttgaccggg
      961 gtgagcggct cgtcggcctc gctgcgcatg aagtcgatga actggtcgat gatcgcgttc
     1021 atctcgccga catcctcgac catcgcgcgg cgcgtgccgg gatccacctt ctcgtcgagc
     1081 atctcgacct cgaggcgcag gcgcgagagc ggggtgcgca ggtcgtggga gacgccggcg
     1141 aggaaggtcg cgcggtcgcg ctggctcttc tgcagcccct gcatcatttg attgaatgcg
     1201 cgcgccacgc cgcgaatctc ggacggcccc gattcctcca cgggcggcgg atcgacgccc
     1261 ttgccgaaag tctccgcggc gcgcgcgagc tccgaaaggg gcctcgtcag cttccacacg
     1321 atgaagaagg tcgcgagaat cgcgatgagc aggccggcga tgccccagga gatgagcgcc
     1381 gtcgccggac cgcgttcgat gcgtccgcgc ggaaacgcga tccagaagtc gcgctccccg
     1441 gccgggagcc ttacccacag gacctgggga ggcatttcgc ccgtttgcgc cgcggcgccg
     1501 cgaacgtaga cgtccgcctg ggggccgaag agctcgcgga tgcggtcgcg aaagagctct
     1561 atggcgggca cgtccgcggc ggggcgcatg cgctcggtgc cgcgcaccgg ggagatgcgg
     1621 atgccatcct tctcggcgat gtgcccgatg aattcctgtt gctgcggcgg ggtcatcgtc
     1681 tgcagcgcgg cgctgatggt cttcaggtgg ctcacgaatt ggccgatgcc cagcgccatg
     1741 cgcggctgcg tcacgtgctc gtggaggatc cacagggatg ccgcctggga gaggatgagc
     1801 gccgcgacga ggacgatca
//
LOCUS       DASVII010000075         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_291705,
            whole genome shotgun sequence.
ACCESSION   DASVII010000075 DASVII010000000
VERSION     DASVII010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291705"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VF309_00955"
     CDS             complement(<1..827)
                     /locus_tag="VF309_00955"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7465178.1"
                     /translation="MLTFLPPLARGIVAALLLGINTLFWCSLILLLALVKLAIPVKPI
                     RVKIDRALNGIATRWVAGNSGWMRLTQRTQWDVAGLDELEYRAWYMVNCNHQSWVDII
                     VLQHLLNRRIPMLKFFLKRQLIYVPVIGLAWWALDFPFMRRHSPAYLRAHPEKRYEDL
                     EASRRACEKFALIPTSVMNFPEGTRFRPAKHRAQQSPYRHLLKPKAGALALALAVLGD
                     KFDSMLDVTIVYPDGAPTFWQFLCGNVPRIVVRARRLPIPRELRTGDYAGDPKFSKV"
     gene            complement(846..>1601)
                     /locus_tag="VF309_00960"
     CDS             complement(846..>1601)
                     /locus_tag="VF309_00960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7465179.1"
                     /translation="GLAFVFNLFVTMIAPVFIAPLFNKYEPLPEGPISKAVLSLARGN
                     EIPTDHVGWFDASKQTTRISANVSGLFGTSRVSLNDNLMNKTSLPEIKAVLGHEMGHY
                     VLNHPLRLTIYLSLVYLVGLALLHLAMDRALARWGGRLKLGDRADPAALPLAIAILSV
                     FQLFATPVLNGIIRQAESEADAYGLNAAREPYGFAMAAMRLSTYRKISPGPVEEMLFY
                     DHPSGYDRVHRSMTWLKENPLPELAAEAAGSSK"
BASE COUNT          304 a          491 c          545 g          261 t
ORIGIN      
        1 aagaccttgc taaatttcgg atccccggcg taatcacccg tgcgcagctc ccgcggaatg
       61 ggcaatcgac gtgcgcgcac gacgatcctc ggcacgttgc cgcaaagaaa ctgccagaag
      121 gtcggcgcac cgtccggata gacgatggtg acgtcgagca tcgaatcgaa cttgtctccg
      181 aggacggcga gagcaagcgc cagcgcgccc gccttcggct tgagcaagtg acggtacggc
      241 gactgctgcg cgcgatgctt cgcgggcctg aaacgcgtgc cctcggggaa gttcatcacg
      301 ctggtcggga tgagcgcgaa tttctcgcag gcgcggcgcg acgcttcgag atcctcgtag
      361 cgcttctccg ggtgcgccct gagataggcg ggggagtggc gccgcatgaa cgggaaatcc
      421 aatgcccacc aggcgagccc gatgaccggc acgtagatga gctggcgctt gaggaagaat
      481 ttcagcatcg ggatgcgccg gttcagcagg tgctggagca cgatgatgtc gacccacgac
      541 tggtggttgc agttgaccat gtaccaggcg cgatactcga gctcgtccag cccggcgaca
      601 tcccactgcg tgcgctgggt gagccgcatc cagccactgt tgcccgcgac ccagcgagtg
      661 gcgatgccgt tcagcgcgcg gtcgatcttc acgcgaatgg gcttcaccgg aatggcgagc
      721 ttgacgagcg ccagcaagag gatcagggag caccagaaaa gcgtgttgat cccgagcagc
      781 agcgccgcga cgatgcctcg tgccaatgga ggaaggaagg tgagcatttc gcgacgcggc
      841 ggatcttact tggagctgcc cgccgcttcc gcagcgagct ccggcagcgg attctccttg
      901 agccacgtca tggagcggtg cacgcgatcg tagccgctcg gatggtcgta gaagagcatc
      961 tcctcgaccg ggcccggcga gatcttgcga taggtggata gccgcatcgc cgccatcgcg
     1021 aagccgtagg gctcgcgcgc cgcgttgagg ccgtaggcgt cggcctcgct ctcggcctgc
     1081 cggatgatgc cgttcaggac cggcgtcgcg aatagctgga acaccgacag gatcgcgatc
     1141 gccagcggca gcgccgccgg atcggcgcga tcgccaagct tgagcctccc accccagcgc
     1201 gcgagcgcac gatccatggc gagatgaagc agcgccaacc cgacgaggta cacgagcgag
     1261 agatagatgg tgaggcgcag cgggtggttc agcacgtagt gccccatctc gtggccgaga
     1321 accgccttga tctccgggag cgacgtcttg ttcatcaggt tgtcgttcag gctcacgcgc
     1381 gaggtgccga aaagcccgga gacgttcgcg ctgatgcgcg tggtctgctt cgaggcgtcg
     1441 aaccacccga cgtggtcggt cggaatctcg ttgccgcgcg cgagcgacag gaccgccttg
     1501 ctgatcggcc cttccggcag cggctcgtac ttgttgaaga gcggcgcgat gaacacgggc
     1561 gcgatcatcg tgacgaagag gttgaagacg aacgcgagcc c
//
LOCUS       DASVII010000076         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_614968,
            whole genome shotgun sequence.
ACCESSION   DASVII010000076 DASVII010000000
VERSION     DASVII010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614968"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VF309_00965"
     CDS             complement(<1..960)
                     /locus_tag="VF309_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7465180.1"
                     /translation="MAIASINPANGETVKTYREMTPQEAAAAVDEAHETWKSWRATSF
                     AERARLMKKTSAILRGRKNELAKLMATEMGKPIKQGVAEAEKCALACDYYADNAEAIL
                     APQAIATEATRSYVAFEPLGVVLAVMPWNFPLWQVYRFAAPALMAGNVGVLKHASNVP
                     GAALAIESMFLEAGFPKGAFRTLLVGSAGVQGVIEHRHVRAVTLTGSTPAGRSVASQA
                     GQVLKKSVLELGGSDPYVVLEDADLELAAQACVNSRLINAGQSCIAAKRFIVVAPVLR
                     EFTDRFVALMKARKVGDPFDPATDVGPLARHDLREGLHKQVLAA"
     gene            complement(973..1428)
                     /locus_tag="VF309_00970"
     CDS             complement(973..1428)
                     /locus_tag="VF309_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7465181.1"
                     /translation="MSNARFMGMLSLALLALAMAARAGPDPAVLSYKTPADLKWTESA
                     MYPGLKSAVLYGDPGKPGPYAVRNRFSPGSFSRPHFHPNDRYILVLSGTWWVGTGDKF
                     DPESTTPMPAGSFVVHYGGKVHYDGAKVGECELLIYGDGPGTSTRVGGS"
     gene            1635..>1984
                     /locus_tag="VF309_00975"
     CDS             1635..>1984
                     /locus_tag="VF309_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX7465182.1"
                     /translation="MKKPNAKKRQDLEARRGAPLATPTDLKKAATKDIAGAMNAILAD
                     VFALYLKTKNFHWHMSGPHFRDYHLLLDEQADQIYAMADPIAERIRKVGGSTLRSIGH
                     IARTQRILDNDADYV"
BASE COUNT          346 a          674 c          630 g          334 t
ORIGIN      
        1 cgcggcgagc acctgcttgt gaaggccctc ccgcaggtcg tgcctcgcga gcggcccgac
       61 atcggtcgcc gggtcgaagg gatccccgac cttgcgcgcc ttcatcaacg cgacaaagcg
      121 atccgtgaac tcgcgaagga ccggggccac gacgatgaaa cgcttcgcgg cgatgcagct
      181 ctgccccgcg ttgatgaggc gcgagttgac gcaggcctgc gcggcgagct cgaggtccgc
      241 gtcctcgaga acgacgtagg gatccgagcc gccgagctcg aggacggatt tcttcaggac
      301 ctggcccgcc tgggaagcga ccgaccttcc cgccggcgtg ctcccggtca gcgtgaccgc
      361 gcgcacgtgg cgatgctcga tcacgccttg cacgcccgcg ctgccgacga gcagcgtgcg
      421 gaaagcccct ttcggaaatc ccgcctcgag gaacatcgac tcgatcgcga gcgcggctcc
      481 gggcacgttc gacgcgtgct tcagcacgcc cacgttgccg gccatcagcg ccggcgccgc
      541 gaatcgatac acctgccaga ggggaaaatt ccagggcatc accgcgagca ccacgccgag
      601 cggctcgaac gcgacgtacg accgggtcgc ttccgtcgcg atggcctgcg gcgcgaggat
      661 cgcctcggca ttgtcggcgt agtagtcgca ggccagggcg cacttctccg cttcggccac
      721 gccctgcttg atgggcttgc ccatctcggt ggccatcagc ttcgcgagct cgttcttgcg
      781 gcctcgaagg attgccgacg tcttcttcat gaggcgcgcc cgctcggcga acgacgtcgc
      841 gcgccacgac ttccacgtct cgtgggcctc gtcgaccgcg gccgcggcct cctgcggcgt
      901 catctcccga taggtcttga ccgtctcgcc gttggcggga ttgatggatg cgatggccat
      961 ggttcagctc cctcagctcc cgcccacgcg cgtggaagtg ccggggccgt cgccgtagat
     1021 gaggagctcg cactccccga ccttcgcccc gtcgtagtga accttgccgc cgtagtgcac
     1081 gacgaagctt cccgcgggca tcggcgtggt gctttccgga tcgaatttat cgccggttcc
     1141 aacccaccat gtgccggaga gcaccaggat gtatcgatcg ttcgggtgga agtgcggacg
     1201 gctgaagctg cccggtgaaa acctgttgcg cacggcatac gggccgggct tacccggatc
     1261 gccgtagagc accgcgctct tgaggccggg atacatcgcg ctctccgtcc acttcaagtc
     1321 cgccggcgtc ttgtagctca gcaccgcggg atcggggccc gccctggccg ccatcgcgag
     1381 ggccaacagt gcaagcgata gcattcccat gaagcgcgcg ttgctcatgt ctgtctcctc
     1441 tgtgcgtgga cgagaatgtt tctgcgcgca gcttggccaa ccccgcgcct aggcaagata
     1501 ctctctccgg atgggacgag gtattggcgt tgcgctggaa cgcgcgcatc gaggggatga
     1561 agtagagtaa ttccacggac ggtgccttcc gggcccgttc cattccacgt tgtcgatcga
     1621 aaggacacca agtcgtgaag aaacccaatg cgaagaagag gcaggatctc gaagccaggc
     1681 gcggcgcgcc gctcgccacc ccgaccgacc tgaagaaggc ggcgacgaag gacatcgccg
     1741 gcgcgatgaa cgccatcctg gcggacgtgt tcgcgctcta cctgaagacc aagaacttcc
     1801 actggcacat gagcggaccg catttccgcg actaccacct gctgctggac gaacaggccg
     1861 accagatcta cgcgatggcg gacccgatag ccgaacgcat ccgcaaggtg ggcggctcca
     1921 cgctcaggtc gatcggccac atcgcccgta cccagcgaat cctggacaat gatgccgact
     1981 atgt
//
LOCUS       DASVII010000077         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_543382,
            whole genome shotgun sequence.
ACCESSION   DASVII010000077 DASVII010000000
VERSION     DASVII010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543382"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1429
                     /locus_tag="VF309_00980"
     CDS             203..1429
                     /locus_tag="VF309_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX7465183.1"
                     /translation="MSVIKAFPRWENFETVSNPGAQVTSTESQLTALRCLQSEHPFWR
                     CRLLAGFEQGAFSREDLRYVFSQYHLYSSSFTRFIAAVMTSCESDLFRAQLSENLWEE
                     GGGCAPERRHAQIFRNFLKTSLGIEDVTRIDYAPHTRSFVREYLAQAQHLEPMAAAAF
                     LALGTEGIVSRMYQIMRTGLVRAGIAESELEFFDIHIACDDAHAATLENLMMSYAGRP
                     GWFDACATALNRALELRAEFFDNLFDALQRSRLESMLSRMQNRESLAREVDDAALCHR
                     PGCETIAMYSNEVEKLNIKFTVERLPLAAEVLDPRMVRIPAGKFNEKHKHAHETLIHI
                     LEGSGQVVIDDRVIPVRVGDSVIVPRWAMHQTQNLGATEMRFLAVTDFKLSQRAYLGD
                     PTDYRLHVDVDGKRHR"
BASE COUNT          266 a          500 c          503 g          257 t
ORIGIN      
        1 taagtgaggc ccgcggtcta gtagtttcgc cgggttaacc ctggggatag cttggcgctg
       61 cgcgtgcaca gcgggaggac atgaaaccaa cgtcgaaggt cgtggcggtg cgggtctccg
      121 cgcggtggca cgcgaccagg cgacaccggg tgacgctcgg ttcaggagtc acctcgaagt
      181 aatacacagg aggagaatac tcatgagcgt tatcaaggca tttccccgct gggagaattt
      241 tgaaaccgtg tccaaccccg gagcgcaggt cacgtcgacc gagagtcagc tgaccgcgct
      301 gcgttgcctt cagagcgagc acccgttctg gcgttgtcgg ctgctcgcag gcttcgagca
      361 aggcgcgttc tcgcgcgagg atctgcgcta cgtgttctcg cagtatcacc tctactcgtc
      421 gagcttcacg cggttcatcg cggcggtgat gaccagctgc gagagcgatc tgttccgcgc
      481 ccagctgtcg gagaacctgt gggaagaggg cggcggatgc gcgccggagc gccgccacgc
      541 gcagatcttc cgcaacttcc tgaagacctc gctcggcatc gaggacgtga ccaggatcga
      601 ctacgcgccg cacacccgga gcttcgtgcg cgaatatctc gcccaggccc agcacctcga
      661 gccgatggca gcggccgcgt tcctcgcgct cgggaccgag ggcatcgtgt cgcggatgta
      721 ccagatcatg cggaccgggc tggtccgtgc aggcatcgcg gagagcgagc tcgagttctt
      781 cgacatccac atcgcgtgtg atgacgcgca tgccgccacg ctcgagaacc tcatgatgtc
      841 gtacgccggc cggccgggct ggttcgacgc gtgcgccacc gcgctgaacc gcgcgctcga
      901 gctgcgcgcc gagttcttcg acaacctgtt cgacgcgctg caacggtcgc ggctcgagtc
      961 gatgctctcg cgcatgcaga accgcgagtc gctcgcgcgc gaggtcgatg acgccgcgct
     1021 gtgtcaccgg cccggctgcg agacgatcgc gatgtattcc aacgaggtcg agaagctgaa
     1081 catcaagttc acggtcgagc gcctgccgct cgcggccgag gtgctcgatc cccggatggt
     1141 gcgcatcccc gccggcaagt tcaacgagaa gcacaagcac gcgcacgaga ccctgatcca
     1201 catcctcgag ggcagcggcc aggtcgtgat cgacgaccgc gtgatcccgg tccgggtcgg
     1261 cgatagcgtg atcgtgccgc gctgggcgat gcaccagacc cagaacctgg gcgcgaccga
     1321 gatgcggttc cttgccgtca ccgacttcaa gctcagccag cgcgcgtacc tcggggatcc
     1381 gaccgactat cgcctgcacg tcgacgtcga cggcaagcgg caccgctagc ctcgcgcctt
     1441 gcgtgggtgc gtaggctcgg cctatatcgt gcccatgccg aaccgcctcg cgggggagag
     1501 ctcgccgtac ttgcggcaac atgccg
//
LOCUS       DASVII010000078         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_147621,
            whole genome shotgun sequence.
ACCESSION   DASVII010000078 DASVII010000000
VERSION     DASVII010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147621"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1177)
                     /locus_tag="VF309_00985"
     CDS             complement(206..1177)
                     /locus_tag="VF309_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7465184.1"
                     /translation="MSPSLRPLAVIAASVLSLSVHVARADWPEHAVRWVVPFSAGGAN
                     DLMARAAAEGVSKRIKQPVVIENKPGAGAAIGAEYVAKSKPDGYTFLIGAAGTITNSM
                     IRKDMPYQDSDLVPVGMIAVAPSVIVVHPSIPASNMKEFIAWANGQGDKGVNWATAGT
                     ASTPHFVAEMLKEAGVTNLNIIPYKSGSESVTAVISNNVAATSEASIVVLPLIKAGKL
                     KAIGTTYDTRISAYKAIPTTKEQGYPGVKIGHWAGLYAPKGTPQPIIDRMNAELLAVV
                     KSEDFADKLVPQGIEPAPYTLATYNAFLNAERERLGKIVKSAKMQAD"
     gene            complement(2337..2774)
                     /locus_tag="VF309_00990"
     CDS             complement(2337..2774)
                     /locus_tag="VF309_00990"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HEX7465185.1"
                     /translation="MVQRIVWIFSLAGAFIFAGLTFVLFSGESHVRIPAIAGLVTFGV
                     VVLSYLGGIEGGLALHDQVGTEKTRAVSLCLSTLPALAAWGVLWLGSTQQQIGASIAL
                     FIAVWCADLWLARQGLIPSWFVDMRTAVTALVCVILGIAYWLL"
     gene            complement(2774..>3630)
                     /locus_tag="VF309_00995"
     CDS             complement(2774..>3630)
                     /locus_tag="VF309_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7465186.1"
                     /translation="GGPLDTVARLLGQKVSESTRQPVIVDNKPGAGGNIGADAVAKAA
                     PDGYTILMGAVATHAINPTLYAAIPYDPVKDFIPVTQIASTPNVLVVNPAVPAANVRE
                     FIAYAKANPGKLNFGSGSTGSAGHLAGELFKTMAGIDMTHVPYKGAAPAMNDLIGGQI
                     QLMFDNLSSSLAQMRAGKVRALAVTTARRSALAPDLPTIAESGLPGFDISTWFGIFVP
                     ARTPGEVVDRLHGEFTKALAAPDIREKMLNLGAEPVGSTPAEFAAYIRSEGDKYARVI
                     RASGARAD"
BASE COUNT          613 a         1154 c         1223 g          640 t
ORIGIN      
        1 gccatcggtg ccgacgaaat cggtgtcgca gaatcgacag accgcttttg cgcgatcctg
       61 ctcgcgcccg ctccacaggt tgcagccagc aaaccggcaa aacacggccg gcgttccggc
      121 attcgcgcct tcgccctgga gcgtgtagaa gatttccttt acggagtaca gggccgtcct
      181 cgcgcctcgt gccttcgacc cttcctcaat cggcctgcat cttcgcggac ttcacgatct
      241 tgccgaggcg ctcgcgctcg gcgttgagga acgcgttgta ggtggcgagc gtatagggcg
      301 cgggctcgat gccctggggc acgagcttgt cggcgaaatc ctcgctcttc accaccgcga
      361 gcagctccgc gttcatgcgg tcgatgatgg gctgcggcgt tcccttcggc gcgtagagcc
      421 ccgcccaatg cccgatcttc actcccggat agccctgctc cttggtggtg ggaatggcct
      481 tgtacgcgct gattcgcgtg tcgtacgtgg tgccgatggc cttgagcttg cccgccttga
      541 tgaggggaag cacgacgatg ctcgcctccg acgtggcggc gacgttgttc gagatgaccg
      601 ccgtcacgct ctcgctgccg ctcttgtacg ggatgatgtt gaggttcgtc acgcccgctt
      661 ccttcagcat ctccgccacg aagtgcggcg tgctcgccgt gcccgcggtc gcccagttca
      721 cgcccttgtc gccctggcca ttcgcccacg cgatgaattc cttcatgttg ctcgcgggaa
      781 tgctcgggtg cacgacgatc accgacggcg ccacggcgat catgccgacg gggacgaggt
      841 cgctgtcctg gtagggcatg tccttgcgga tcatgctgtt ggtgatcgtt cccgcggcgc
      901 cgatgaggaa cgtgtagccg tcgggcttgg acttggcgac gtactccgcg ccgatggccg
      961 cgcccgcgcc gggcttgttc tcgatcacga ccggctgctt gatgcgcttc gacacgccct
     1021 cggcggccgc gcgcgccatg aggtcgttcg cgcccccggc cgagaagggc acgacccacc
     1081 tcaccgcgtg ctcgggccaa tcggcgcggg cgacgtggac cgagagcgag aggacggatg
     1141 ccgcgatcac ggcgagcggg cgaagggatg gggacatgga agcttctccg gggaagacgg
     1201 ggcgcggggg aaacgatcgc aattatcgtg caaaaggggt tgacgatcga attgctgcat
     1261 ggcagcatcc gatcatcaag ggtacacgag gttttttccg agagacatga agaccaccac
     1321 cttcatttac cgaccgacca aggcgcgctg ggcagccttt gggtcctgcc atgcgcaggg
     1381 ctccaagcgc ctggtaatgg attgcgccct ctccgggaga accgcggaat aaagcatcga
     1441 tcatcgatct gaattccccg cgaagcccgg agaccgaaaa cctccgggct tcgtgctttt
     1501 gcaacggcaa tgatcactgg gaatacgtcg atgagagcgt tgagattgaa gtggaagtgg
     1561 cgagcgcgcc tcgcgcgcta cgagcaggac gcgcgccatg cgatgcgcgc gtggttgatg
     1621 atttgagagc tcggctccgt caaggggccg tccggcaact tcgtgggctg tgcgcggccc
     1681 gcctaacgag gatttcgggt tgccgcccgc cagcgcctgt cgagggacgc ctcggggcgc
     1741 ggggcgggtt cgatgacgta gcggccggtc cttcggggcc ggccgcggtc agaattttct
     1801 gtttcttcgc catccatcga actcgggatg aagctcgacc cggcgcgatc gttccttggg
     1861 acgggaggga gctcgctgca acccattccg ctcgaactgt tggcgacctc tcccgatcga
     1921 ccggcagtga cgcgcgcctc tcgagcttca tcccaagagt tcgatggatg gctctgcgtg
     1981 cccattccga ccgtcccggg gtcttagatg tgaatcgcca agaagaacgg cgatgcgcga
     2041 cgcgtccagg atgtgcagcc cgcgccaggg ggctaccagt gatctatcgg cggggatgcg
     2101 aggagcatca gagcggaaca caataaattt tggatggtgg actgcacagg cacggcccga
     2161 cacatgagag cctcattgaa gtgttggggt gaagctcgag aggcgcgcgt cacttcgggt
     2221 cgaaaggaag aagacgccgc cagttcgagc ggaatgggtt gcagcgaggt ccctcccgct
     2281 tccaggaacg atcgcgccgg ctcgagcttc acccccactt caatgaggcg atagcgtcaa
     2341 agaagccagt acgcgatccc gaggatcacg cagaccagcg cggtgaccgc ggttcgcatg
     2401 tcaacgaacc aggacgggat caatccctgg cgagccagcc agagatcggc gcaccacacg
     2461 gcgatgaaga gcgcgatcga tgcgccgatc tgctgctggg tcgaccccag ccacagcacg
     2521 ccccacgccg cgagcgccgg cagcgtgctc aggcataacg agacggcgcg cgtcttctcg
     2581 gtgcccacct ggtcgtgcag cgcgaggccg ccctcgatgc cgccgaggta ggagagcacc
     2641 acgaccccga acgtgacgag tcccgcgatc gccgggatgc gcacgtgcga ttcgcccgaa
     2701 aacagcacga acgtgaggcc cgcgaagatg aacgcgccgg cgagcgagaa gatccatacg
     2761 atgcgctgga ccatcagtcg gccctcgcgc ccgaggccct gatgacgcgc gcgtacttgt
     2821 cgccctcgga gcggatgtag gcggcgaact ccgcgggcgt gctcccgacg ggctcggcgc
     2881 cgaggttcag catcttttcg cggatatcgg gcgcggcgag cgccttcgtg aactcgccgt
     2941 gcagccgatc gacgacttcg cccggcgtgc gcgcgggcac gaagatcccg aaccacgtgc
     3001 tgatgtcgaa gccgggcaat cccgactcgg cgatggtcgg caggtccggc gcgagcgccg
     3061 agcgcctggc ggtcgtcacg gcgagcgcgc gcaccttgcc cgcgcgcatc tgcgcgagcg
     3121 aggagctcag gttgtcgaac atcaactgga tctgcccgcc gatgaggtcg ttcatggcgg
     3181 gcgcggctcc cttgtacggc acgtgggtca tgtcgattcc cgccatcgtc ttgaagagct
     3241 ctcccgcgag gtggcccgcg ctcccggtgc tgcccgagcc gaaattcagc ttgcccggat
     3301 tcgccttggc gtacgcgatg aattcacgca cgttggcggc gggcaccgcg ggattcacga
     3361 cgagaacgtt cggcgtcgag gcgatctggg tcacggggat gaagtccttg accggatcat
     3421 acggaatggc ggcgtagagc gtgggattga tggcgtgggt cgcgaccgcc cccatgagga
     3481 tcgtgtagcc gtcgggcgcg gccttcgcca cggcatcggc gccgatgttg cctcccgcgc
     3541 cgggcttgtt gtcgacgatg acgggctggc gcgtgctctc ggataccttc tgcccgagca
     3601 ggcgcgcgac cgtgtcgagc ggtccgccgg
//
LOCUS       DASVII010000079         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_39999, whole
            genome shotgun sequence.
ACCESSION   DASVII010000079 DASVII010000000
VERSION     DASVII010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39999"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /gene="tsf"
                     /locus_tag="VF309_01000"
     CDS             complement(<1..377)
                     /gene="tsf"
                     /locus_tag="VF309_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7465187.1"
                     /translation="MAEITAALVKELRERTGLGMMECKKALEEAGGDLQKAEELLRIK
                     SGAKASKAAGRVAADGAIGTYLAPDRKLGALVEVNAETDFVAKNPDFAAFAEGVAKLV
                     AEKNPADVAALSNLSLGSETVESA"
     gene            complement(471..1220)
                     /gene="rpsB"
                     /locus_tag="VF309_01005"
     CDS             complement(471..1220)
                     /gene="rpsB"
                     /locus_tag="VF309_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7465188.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRYWNPKMAPYIFGARNKIHIINLEK
                     SLPMYQDALKYVRQLTANKGQILFVGTKRQAREIVKEEAQRAGAPYVDYRWLGGMLTN
                     FKTVKGSIKRLKEMDAMIADGSMERLSKKEQLLNGREREKLERALGGIKDMGGGPDAL
                     FVIDVGFHKIAVTEAKKLGIPIVGVVDSNNSLDGIDYVIPGNDDSTRAIRLYARGVAD
                     AILEGRSQVISELVAPQSSEDFVEVEEAEKQ"
     gene            1482..2303
                     /gene="map"
                     /locus_tag="VF309_01010"
     CDS             1482..2303
                     /gene="map"
                     /locus_tag="VF309_01010"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7465189.1"
                     /translation="MHQPLKSPEDLAKMKVAGRLASEVLDFIAPHVKPGVTTGALDKL
                     CHDYMVNVQGTIPAPLNYAPPGHRPYPKSVCTSVNHQVCHGVPGDKVLKNGDIVNIDV
                     TVIKEGWHGDTSRMFYVGEPSVQARRLCEVTYECMWKGIAMVRPGAALGDIGSAIQKH
                     AEANGFSVVREFCGHGIGKRFHEEPQILHYGRPGQGLKLVTGMTFTVEPMINAGKRDI
                     RQLADGWTIVTADHSLSAQWEHTVLVTDDAFEILTVSAGTPAPPAFVEQRATVVS"
     gene            2330..>3144
                     /locus_tag="VF309_01015"
     CDS             2330..>3144
                     /locus_tag="VF309_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7465190.1"
                     /translation="MARQTPPSETRAWIKASRDELERRYLRRPDPARTLAAHAAFVDQ
                     VLQRLWKETISDRDIALVAVGGYGRGALFPNSDVDVLVLLPDGRRPDAAVERFIGALW
                     DSGLEPGHSVRTVAESVEEAAKDVTVDTSLIEARLVAGNPALVEDLEKRLRARRDLRD
                     FFEAKFQEQKRRHDRFQDAAYNLEPNIKESPGGLRDLQMVLWLARAAGLGQSWKALAQ
                     EGLITAREAAAIAANERVLLDLRIRLHYLAGRREDRLVFDHQIEIARQLKLKS"
BASE COUNT          505 a         1080 c         1013 g          546 t
ORIGIN      
        1 gcggattcca ccgtctccga tccgagggag aggttggaga gcgccgccac gtcggccggg
       61 ttcttctcgg ccacgagctt cgccacgcct tcggcgaacg ccgcgaagtc ggggttcttg
      121 gcgacgaagt cggtctcggc gttcacctcg accagcgcgc ccagcttcct gtcgggcgcg
      181 aggtacgtgc cgatggcgcc gtcggccgcc acgcggcccg ccgccttgga ggccttcgcg
      241 ccgctcttga tgcgcagcag ctcctcggcc ttctgcaggt ctccgcccgc ttcctcgagc
      301 gccttcttgc attccatcat gccgagcccg gtgcgctcgc gcagttcctt gaccagtgcc
      361 gccgtgattt ccgccatgtt cccgctccgt gatgacaagt tgcaaaaaag gggctcgatc
      421 gccccttttc cgccgggctc ttcgacagag cccctattcg atggaaccag ttactgcttt
      481 tccgcttcct cgacctcgac gaagtcctcg gagctctgcg gcgcgacgag ctcgctgatg
      541 acctggctgc ggccctcgag gatcgcgtcc gccacgcccc gcgcatagag gcggatggcg
      601 cgcgtggaat cgtcgttgcc gggaatcacg tagtcgatgc cgtcgagcga gttgttcgag
      661 tcgacgacgc ccacgatcgg gatgccgagc ttcttcgctt cggtgaccgc gatcttgtgg
      721 aagcccacgt cgatcacgaa gagcgcgtcg gggccgccgc ccatgtcctt gatgccgccg
      781 agggcgcgct cgagcttctc gcgctcgcgc ccgttcagca gctgctcctt cttcgacagg
      841 cgctccatgg agccgtcggc gatcatcgcg tccatttcct tcaggcgctt gatcgagccc
      901 ttcaccgtct tgaagttggt gagcatgccg cccagccagc gatagtccac gtagggcgcg
      961 ccggcgcgct gggcttcttc cttcacgatc tcgcgcgcct ggcgcttggt gccgacgaac
     1021 aggatctgcc ccttgttcgc ggtgagctgg cgcacgtact tgagcgcgtc ctggtacatc
     1081 ggaagcgatt tctcaaggtt gatgatgtgg atcttgttgc gggccccgaa gatgtacgga
     1141 gccatcttgg gattccagta gcgcgtctgg tggccgaagt gaacgccggc ctccagcatc
     1201 tggcgcatgg tgacggacat gcaattactc cttggtaagg ttggcctccc ccgcgacgtg
     1261 accggaagtt cgattgcgaa cgaccagcac cttgaccatt ggcggggtgc gaatttccga
     1321 agtcgaaaat gaccccggat agctggctat tatagcccaa aaatgggctt ggattcaaac
     1381 atttcccggc gcttgcggag taatcggcga tgcggaatcc gtacgctcgg cgatgctcgc
     1441 gcctgacggt ataatccgca ctcgagacca tgctccagcc cctgcaccag ccgttgaaat
     1501 cgcccgagga cctcgcaaag atgaaggtcg cgggccgcct cgcgtccgaa gtcctcgact
     1561 tcatcgcccc gcacgtgaag cccggggtga cgaccggcgc cctcgacaag ctctgccacg
     1621 actacatggt gaacgtgcag gggaccattc ccgcgccgct caactacgcg ccgccgggcc
     1681 atcgccccta tccgaaatcg gtctgcacct cggtgaacca ccaggtgtgc cacggcgtgc
     1741 ccggcgacaa ggtgctgaag aacggcgaca tcgtgaacat cgacgtgacc gtcatcaagg
     1801 aaggctggca cggcgacacg agccgcatgt tctacgtggg cgagccctcg gtgcaggcgc
     1861 ggcgactgtg cgaggtcacc tacgagtgca tgtggaaggg gatcgcgatg gtgcgcccgg
     1921 gcgcggccct cggcgacatc ggctccgcga tccagaagca cgcggaggcc aacggtttca
     1981 gcgtcgtgcg cgagttctgc ggtcacggca tcggcaagcg tttccatgag gagccgcaga
     2041 tcctgcacta cgggcgcccg ggacaaggcc tgaagctcgt gaccggcatg accttcacgg
     2101 tcgagccgat gatcaacgcg ggcaagcgcg acatccgcca gctcgccgac ggctggacca
     2161 tcgtcaccgc cgatcactcg ctctcggcgc agtgggagca cacggtgctc gtcaccgacg
     2221 acgcgttcga gatcctcacc gtctcggcgg gcactcccgc gccgccggcc ttcgtcgagc
     2281 agcgcgcgac cgtcgtctcc tgagcaccac gacccttccc gcgcagggca tggcgcggca
     2341 gacgccgccc tcggagacgc gtgcgtggat caaggcctcg cgcgacgagc tcgagcgccg
     2401 ctaccttcgc cgtcccgatc ccgcgcgcac gctcgcggcg cacgcggctt tcgtcgacca
     2461 ggtgctgcag cggctgtgga aggagacgat ctccgatcgc gacatcgcgc tggtcgccgt
     2521 gggcggttac gggcgcggcg cgctctttcc gaattccgac gtcgacgtgc tcgtgctgct
     2581 gcccgatggg cggcgccccg acgccgcggt cgagcgcttc atcggcgcgc tctgggatag
     2641 cggcctcgag cccggccaca gcgtgcgcac cgtggccgaa tcggtcgagg aagccgccaa
     2701 ggacgtgacc gtggacacga gcctcatcga ggcgcggctc gtcgcgggca acccggcgct
     2761 cgtcgaagac ctggagaagc gcctgcgcgc gcggcgcgac ttgcgcgatt tcttcgaggc
     2821 caagttccag gagcagaagc gccgccacga ccgcttccag gacgccgcct acaacctcga
     2881 gcccaacatc aaggaaagcc ccggggggct tcgcgacctg cagatggtgc tgtggctcgc
     2941 gcgcgccgcg gggctcggcc agtcgtggaa ggcgctcgcg caggaaggcc tcatcacggc
     3001 gcgcgaggcc gcggcgatcg ccgccaacga gcgcgtgctg ctggacctgc gcattcgcct
     3061 tcactatctc gccggccggc gcgaggaccg cctcgtcttc gaccaccaga tcgagatcgc
     3121 acgccagctc aagctgaagt cgag
//
LOCUS       DASVII010000080         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_471532,
            whole genome shotgun sequence.
ACCESSION   DASVII010000080 DASVII010000000
VERSION     DASVII010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471532"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /gene="rpsM"
                     /locus_tag="VF309_01020"
     CDS             <1..212
                     /gene="rpsM"
                     /locus_tag="VF309_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7465191.1"
                     /translation="LREQVGKFTVEGDLRRETSMNIKRLMDLGCYRGVRHKKGLPVRG
                     QRTRTNARTRKGPRKAVRLSKAATV"
     gene            237..632
                     /gene="rpsK"
                     /locus_tag="VF309_01025"
     CDS             237..632
                     /gene="rpsK"
                     /locus_tag="VF309_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7465192.1"
                     /translation="MAKAPAATRARKKAKKVVSEGIAHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAESAGRAAQEHGVKNLEVRIKGPGPGRESAVRAL
                     NALGLKIVSIADVTPVPHNGCRPPKRRRM"
     gene            684..1304
                     /gene="rpsD"
                     /locus_tag="VF309_01030"
     CDS             684..1304
                     /gene="rpsD"
                     /locus_tag="VF309_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7465193.1"
                     /translation="MARYTGPKCKLSRREGTDLFLKSARRSLDTKCKLDSRPGQHGAK
                     STRISEYGTQLREKQKIRRMYGVLERQFRTYFDKAIQRRGATGENLLQMLESRLDNVV
                     YRMGFASTRAEARQLVSHKAIQVNGKPVNIPSYLVKATDTVAVREKSRAQLRIKASLD
                     LAEQNGFPSWVEVDPKNFKGVFKALPDRAEFANDVNEQLVVELYSK"
     gene            1362..>1877
                     /locus_tag="VF309_01035"
     CDS             1362..>1877
                     /locus_tag="VF309_01035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7465194.1"
                     /translation="MQSNATNFLKPRIIDVQNLSPTHAKVVMEPFERGYGHTLGNALR
                     RILLSSMPGYAATEVKIAGVLHEYSTIEGVQEDVVDVILNLKGVVMKLHNRSEATLKL
                     KKNSPGAVTAADIEQTHDVEIINPDHVIAHLTQGGKIDMTIKIEKGRGYVPATSRRQD
                     DTRSIGSILLDA"
BASE COUNT          391 a          630 c          594 g          262 t
ORIGIN      
        1 agctgcgcga gcaggtcggc aagttcaccg tcgaggggga cctgcgccgc gagacgtcga
       61 tgaacatcaa gcgcctcatg gacctcggct gctatcgagg cgtgcgccac aagaagggcc
      121 tgcccgtgcg cggtcagcgc acgcgcacca acgcgcgcac ccgcaaggga ccgcgcaagg
      181 cagtccggct gagcaaggcc gcgacggtct aatcgagatc aggaaggaac caccgaatgg
      241 ccaaggcacc cgcagcaacg cgcgcacgca agaaggcgaa gaaagtcgtc tccgagggca
      301 tcgctcacgt gcacgcgtcg ttcaacaaca cgatcatcac gatcaccgac cgccagggca
      361 acgcgctctc gtgggcgact tcgggcggcg ccggcttcaa gggctcgcgc aagtcgacgc
      421 ccttcgcggc gcaggtggcc gccgaatccg cgggccgcgc ggcccaggag catggcgtga
      481 agaacctcga agtgcgcatc aaggggcccg gccccgggcg cgagtcggcg gtgcgcgcgc
      541 tgaatgcgct cgggctgaag atcgtgtcga tcgccgacgt gacgccagtc ccccacaacg
      601 gttgccggcc gccgaaacgc cgccgcatgt aacgcgagcc ttcgctcgta tcgcgactcg
      661 caggtattga ttaggagaac accgtggcac gttataccgg acccaagtgc aagctctcga
      721 ggcgggaagg caccgacctc ttcctgaaga gcgcgcgccg ctcgctcgac accaagtgca
      781 agctcgactc gcgcccgggg cagcatggcg ccaagtcgac gcgcatctcg gaatacggca
      841 cgcagcttcg cgagaagcag aagatccgcc gcatgtacgg cgttctcgag cgccagttcc
      901 gcacctattt cgacaaggcg atccagaggc gcggcgccac cggcgagaac ctcctgcaga
      961 tgctcgagag ccgcctggac aacgtggtgt accgcatggg attcgcctcc acccgcgccg
     1021 aggcgcggca gctcgtctcg cacaaggcga tccaggtgaa cggcaagccc gtgaacatcc
     1081 cgtcgtacct ggtcaaggcc acggataccg tcgcggtgcg cgagaagtcg cgcgcgcagc
     1141 tgcgcatcaa ggcatcgctg gacctcgccg agcagaacgg attcccgagc tgggtcgaag
     1201 tcgatcccaa gaatttcaag ggcgttttca aggcgctacc cgaccgcgct gagttcgcca
     1261 acgacgtgaa cgagcagctg gtggtcgaac tttattcgaa gtaattcgat gtaacgcgcg
     1321 aggggtcgct ccccgagcgc atctggccga ggtacctgcg aatgcaaagc aacgccacga
     1381 atttcctgaa gccccgaatc atcgatgtcc agaacctttc gccgacgcac gcgaaggtcg
     1441 tgatggagcc cttcgagcgc ggatatggcc acacgctggg caacgcgctg cgccgcatcc
     1501 tgctctcctc gatgcccggc tacgccgcga ccgaggtgaa gatcgccggc gtgctgcacg
     1561 agtactcgac catcgaaggc gtgcaggaag acgtcgtcga cgtcatcctc aacctcaagg
     1621 gcgtggtgat gaagctgcac aaccgctccg aggcgacgct gaagctcaag aagaactcgc
     1681 ccggcgcggt gaccgccgcc gacatcgagc agacccacga cgtcgagatc atcaatcccg
     1741 accatgtgat cgcgcatctc acgcagggcg gcaagatcga catgaccatc aagatcgaga
     1801 agggccgcgg ctacgtgccg gccacctcgc gccgccagga cgacacgcgc tcgatcggct
     1861 cgatcctgct cgacgcc
//
LOCUS       DASVII010000081         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_651230,
            whole genome shotgun sequence.
ACCESSION   DASVII010000081 DASVII010000000
VERSION     DASVII010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651230"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VF309_01040"
     CDS             complement(<1..1135)
                     /locus_tag="VF309_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX7465195.1"
                     /translation="MAAVGTLDPAAQHAPAAEGERAPRAPLPETPLARTPTILQLESV
                     ECGAAALAMILAFYNRHVPLEQMRVDCGVSRDGSKAGGLLRAARAHGMIARGFKKEPD
                     AIAGLPLPAIVFWNFNHFVVLEGFRDGRAWLNDPARGRRSVDAAEFDQAFTGVVLTFE
                     RGPGFEPGGFAPSMWRSLRQYLDGYRASIGAAFFIGVALVIPGLVMPWLLGRFVDEVL
                     VAKLDGVALPLLAGLVVAALARALLIALQARLLMDTFGRAARASARRFFARALALPMT
                     FYSQRSAGEIAARIDLNERVAETISADLARLALNFVTAAFFLALMARLSLQLTSVVVA
                     CLVVELFAWRALARRTAEISQELSVQAGKLAGTATGGLSNIESI"
     gene            complement(1114..1284)
                     /locus_tag="VF309_01045"
     CDS             complement(1114..1284)
                     /locus_tag="VF309_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465196.1"
                     /translation="MADESIFRKAALDRLASPERLDERLGLPAYPRALLGALAALLVA
                     VAAFAIWLLSVR"
     gene            complement(1292..1927)
                     /locus_tag="VF309_01050"
     CDS             complement(1292..1927)
                     /locus_tag="VF309_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7465197.1"
                     /translation="MLNASFLLDLDSHHRRISAAPGERGTLAPPRSARGDVVRKVLFI
                     FSVLTDGDVEWLAKAGERVHVDPGTVLIALGARVEYLYFVLEGRLAIKTKSGDPIALL
                     ESGEIIGEMSLVDPAPTTVSVEVAADTTLLRIPNAVLREKLDSDPGFASRFYRALCVF
                     LADRMRHATQRMGYGAATDDANARDELNDDLLDTVHLAGARFDRMLKRLAG"
     gene            1915..>2477
                     /locus_tag="VF309_01055"
     CDS             1915..>2477
                     /locus_tag="VF309_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7465198.1"
                     /translation="MHSAWRNILAACAALPALALALADDPSATIVQSERADREAFLAQ
                     GAKKEGEVVVYTSLVPEDLAALSAAFEKKYGVKVKGWRAGSEKVLQRALTEARANRND
                     ADVIETNGPELESLYREKVLHPFKSPHLKELMPQALRPHGQWVGTRINMFVQMYNTQL
                     VKKADLPRKYSDLADPRWKGKLGIEAE"
BASE COUNT          420 a          835 c          899 g          323 t
ORIGIN      
        1 tgatgctctc gatgttggac aggccgccgg tcgccgtgcc cgccagcttg cccgcctgca
       61 cggatagctc ctgcgagatc tccgcggtgc gccgcgcgag cgcgcgccac gcgaagagct
      121 ccaccacgag gcacgccacg acgaccgacg tgagctgcag ggagagccgc gccatgagcg
      181 cgaggaagaa ggccgcggtg acgaagttga gcgccaggcg cgcgaggtcc gcggagatgg
      241 tctcggcgac gcgctcgttg aggtcgatcc gcgcggcgat ctcgcccgcg gagcgctgcg
      301 agtagaaggt catcggcagc gcgagcgccc gcgcgaagaa gcgccgggcc gaggcgcgcg
      361 cggcgcgccc gaaggtgtcc atgagcagcc gcgcctgcag cgcgatgagc agcgcccgcg
      421 cgagcgccgc cacgacgagg ccggcgagca gcggcagcgc cacgccgtcc agcttcgcca
      481 ccagcacctc gtcgacgaat cgccccagca gccacggcat cacgagcccc gggatcacga
      541 gggcgacgcc gatgaagaac gccgcgccga tcgaggcgcg gtatccgtcg agatactggc
      601 gcagagatcg ccacatcgag ggcgcgaatc cgcccggctc gaacccgggc ccgcgctcga
      661 aggtgagcac gacgccggtg aacgcctggt cgaattccgc cgcgtccacg ctgcggcgcc
      721 cgcgcgcggg atcgttcagc cacgcgcgcc cgtcgcggaa cccctcgagg acgacgaagt
      781 ggttgaagtt ccagaagacg atcgcgggca gcggcagccc ggcgatggcg tcgggctcct
      841 tcttgaagcc gcgcgcgatc atgccgtggg cccgcgccgc gcgcagcagc ccgcccgcct
      901 tgctgccgtc gcggctcacg ccgcaatcga cgcgcatctg ctcgagcggc acgtggcgat
      961 tgtagaacgc gaggatcatc gcgagcgccg cggcgccgca ttcgacgctc tcgagctgga
     1021 ggatcgtggg cgtgcgcgcg agcggggttt ccggcagcgg cgcgcgcggg gcgcgctcgc
     1081 cttctgccgc gggcgcgtgc tgcgccgccg gatctagcgt accgacagca gccatatcgc
     1141 gaatgcggcg accgccacca gcagcgcggc gagggcgccg aggagcgcgc gcggataggc
     1201 gggaaggccg aggcgctcgt cgagccgctc gggggaggcg agacggtcca gcgccgcctt
     1261 gcggaagatg gattcgtcgg ccatggggcg ctcagcccgc gagacgcttc agcatccggt
     1321 cgaagcgcgc tcccgccagg tgcacggtgt cgaggaggtc gtcgttcagc tcgtctctcg
     1381 cgttcgcatc gtcggtcgcg gcgccgtagc ccatgcgctg cgtcgcgtgg cgcatgcggt
     1441 cggcgaggaa gacgcacagc gcgcggtaga agcgcgaagc gaagccggga tccgaatcga
     1501 gcttctcgcg caacaccgcg ttggggatgc gcagcagcgt cgtgtccgcg gcgacctcca
     1561 ccgagaccgt cgttggcgcc ggatcgacga gcgacatctc gccgatgatc tcgcccgact
     1621 cgaggagcgc gatgggatcg cccgacttgg tcttgatcgc gagccgcccc tcgaggacga
     1681 agtagaggta ctccacgcgc gcgccgagcg cgatgaggac cgtgcccgga tcgacgtgca
     1741 cgcgctcgcc cgccttggcg agccactcga cgtcgccgtc ggtgaggaca ctgaagatga
     1801 agaggacttt tctcacgacg tctccccgcg ccgatcgagg tggtgcgagt gtaccgcgct
     1861 cgcccggcgc cgccgaaatt cggcgatgat gcgaatcgag atcgaggagg aaagatgcat
     1921 tcagcatggc ggaacattct cgcggcctgc gccgcgctac cggcgctcgc gctcgcgctg
     1981 gccgacgatc cctcggccac gatcgtgcag agcgagcgcg ccgaccggga ggcatttctc
     2041 gcccagggcg cgaagaagga aggcgaggtg gtggtgtaca cctcgctcgt tccggaagac
     2101 cttgccgcgc tttcggccgc gttcgagaag aagtacggcg tgaaggtgaa gggctggcgc
     2161 gccggcagcg agaaggtgct gcagcgcgcg ctcacggagg cgcgtgccaa tcgcaacgac
     2221 gcggacgtga tcgagaccaa tggcccggag ctcgagtcgc tctatcgcga gaaggtcctg
     2281 catcccttca agtcgccgca cctgaaggag ctgatgcccc aggcattgcg tccccacggg
     2341 caatgggtcg gcacgcggat caacatgttc gtgcagatgt acaacacgca gctcgtgaag
     2401 aaggcggacc tgccgaggaa atactccgat ctcgcggacc cgcgctggaa ggggaagctc
     2461 ggcatcgagg ccgagga
//
LOCUS       DASVII010000082         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_255737,
            whole genome shotgun sequence.
ACCESSION   DASVII010000082 DASVII010000000
VERSION     DASVII010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255737"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..318
                     /gene="hfq"
                     /locus_tag="VF309_01060"
     CDS             79..318
                     /gene="hfq"
                     /locus_tag="VF309_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX7465199.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVTVPLEHSQDA"
     gene            296..1432
                     /gene="hflX"
                     /locus_tag="VF309_01065"
     CDS             296..1432
                     /gene="hflX"
                     /locus_tag="VF309_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7465200.1"
                     /translation="MNIRKTPSRPRAVLVSLDIGRASKTDERAGEAARLVESAGSQVV
                     EVIRGRRDRPDSSTFAGKGKGEEIGLALLEHKANVVVFDRQLSPAQVRNIERALIDKV
                     PDIRVFDRTDVILDIFAQRARTSEGKLQVELARLEHLYTRLVKGWTHLERQRGSLGKT
                     GGPGEKQIELDRRMIGEQVKKLKERLRKVGTVRDTQRAGRTRSGILRVALVGYTNAGK
                     STLFNRITGAEAYVADQLFATLDTTTRRVYLAADASIALSDTVGFIRDLPHTLVDSFK
                     ATLEETIQADLLLHVVDIANPEHHEQVEAVNAVLAEIGASTVPQILVYNQIDRVPGLA
                     PEIVRDAHGKILNIKVSASTGAGIEGLRDALAESVRDDSLPTAA"
     gene            1493..>2454
                     /gene="hflK"
                     /locus_tag="VF309_01070"
     CDS             1493..>2454
                     /gene="hflK"
                     /locus_tag="VF309_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX7465201.1"
                     /translation="MPPNDPQWGKKNNEGPPDLEEIFRKLQQKVVGLLGLQGRGPGTP
                     SGGGGGNMSSAVGSGVVLVVVLVVLVWLASGFYIVDEGRRGVVLRLGKFHETTMPGPR
                     WHVPYPIESVDVVDVARVRTVEVGYRGNPKNKQPQEALMLTDDENIVDVQLAVQYTLK
                     SPEDYLFNNKAPDDNVLQAAETAIREVVGKSKMDFVIQQGRSEIASRVRVLMQQILDR
                     YKSGINITTVNLLAAAAPEQVQPAFEDVVRAQQDRERFKNEGQAYANDVVPKARGIAA
                     RLIEEASGYRQSVISTAQGDAARFRQILAEYEKAPAVTRERIYL"
BASE COUNT          472 a          819 c          804 g          359 t
ORIGIN      
        1 tgctttgcag taaagtccac ctgcaggcaa cggcccggcg tagaccggga aacccgaaga
       61 gagaaaaggg gcagaacaat gagcaacaag ggccaattac tccaagaccc gttcctcaat
      121 acgttgcgca aagagcatgt accggtctcg atctacctgg tgaacggcat caagctgcag
      181 ggccagatcg aatccttcga ccagtacgtc gtgctcctca agaacaccgt gacgcagatg
      241 gtctacaagc acgcgatctc cacggtggtc ccggcgcggc cggtcacggt cccgcttgaa
      301 cattcgcaag acgcctagcc gccctcgcgc agtcctcgta agcctcgaca tcggccgcgc
      361 ctcgaagacg gacgagcggg ccggggaagc ggcgcgcctg gtggaaagcg cgggctccca
      421 ggtcgtcgag gtgatccgcg gccgtcgcga tcgtcccgat tcgtcgacct tcgccggcaa
      481 gggcaagggc gaggagatcg ggcttgcgtt gctcgagcac aaggcgaacg tcgtcgtctt
      541 cgaccgccaa ctatcgcccg cgcaggtgcg caacatcgag cgcgcgttga tcgacaaggt
      601 ccccgatatc cgcgtgttcg atcgcaccga cgtgatcctc gacatcttcg cgcagcgcgc
      661 gcgcaccagc gagggaaagc tccaggtcga gctcgcccgc ctcgagcacc tgtacacgcg
      721 cctcgtgaaa ggctggacgc acctcgagcg gcagcgcggc tccctcggca agaccggcgg
      781 cccgggcgag aagcagatcg agctcgaccg gcgcatgatc ggcgagcagg tgaagaagct
      841 gaaggagcgc ctgcgcaagg tcggcacggt gcgcgacacg cagcgcgcgg gccgcacgcg
      901 ctcgggcatc ctgcgcgtcg cgctcgtggg ctacacgaac gcgggcaagt ccacgctctt
      961 caatcgcatc accggcgccg aggcgtacgt cgccgaccag ctgttcgcga cgctcgacac
     1021 gaccacgcga cgcgtgtacc tcgccgccga cgcgtcgatc gcgctctccg ataccgtggg
     1081 attcatccgc gaccttccgc atacgctcgt ggattcgttc aaggcgaccc tggaagagac
     1141 gatccaggcc gatctcctgc tgcacgtcgt ggacatcgcc aacccggagc accacgagca
     1201 ggtcgaggcg gtgaacgcgg tgctggcgga gatcggcgcc tcgacggtgc cgcagatcct
     1261 cgtctacaac cagatcgatc gcgtgccggg gctcgctccc gaaatcgtgc gcgatgcgca
     1321 tggtaagata ttgaatatca aggtgagtgc gtccaccgga gcgggtatcg aaggccttcg
     1381 cgacgcgctc gccgagtccg tccgggacga ttccctgccc acggcggctt gataagcgcg
     1441 cgataccccc gactttagag tcgtctccca ctcttctccg aattcattgc cgatgccccc
     1501 gaacgatccc cagtggggaa agaagaacaa cgaaggtccg cccgatctcg aggagatctt
     1561 caggaagctc cagcagaagg tcgtggggct gctgggcctg caaggccgcg gtcccggcac
     1621 gccttcgggc ggcggcggcg gcaacatgag ctcggcggtg ggaagcggcg tggtcctggt
     1681 ggtcgtcctc gtcgtgctgg tgtggctcgc gagcggcttc tacatcgtcg acgagggccg
     1741 ccgcggcgtg gtgctgcgcc tgggcaagtt ccacgagacc acgatgcccg ggccgcgctg
     1801 gcacgtgccg tatccgatcg aatccgtcga cgtcgtcgac gtggcgcggg tgcgcaccgt
     1861 ggaggtcggc taccgcggca atcccaagaa caagcagccg caggaagccc tcatgctcac
     1921 cgacgacgag aacatcgtcg acgtgcagct ggcggtgcag tacacgctga agagccccga
     1981 ggactacctc ttcaacaaca aggcgcccga cgacaacgtg ctgcaggccg ccgagaccgc
     2041 gatccgcgag gtggtcggca agagcaagat ggacttcgtg atccagcagg gccgcagcga
     2101 gatcgcctcg cgcgtgaggg tgctgatgca gcagatcctc gaccgctaca agtcgggcat
     2161 caacatcacc accgtgaacc tgctggccgc cgcagctccc gagcaggtgc agcccgcctt
     2221 cgaggacgtg gtgcgcgcgc agcaggaccg cgagcgcttc aagaacgagg ggcaggccta
     2281 cgccaacgac gtggtgccga aggcgcgcgg catcgcggcg cgcctcatcg aggaggcgag
     2341 cggctaccgc cagagcgtca tcagcaccgc ccagggcgat gccgcgcgct tccgccagat
     2401 cctcgccgaa tacgagaagg cccccgcggt gacgcgcgag cgcatctacc tcga
//
LOCUS       DASVII010000083         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_399720,
            whole genome shotgun sequence.
ACCESSION   DASVII010000083 DASVII010000000
VERSION     DASVII010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399720"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VF309_01075"
     CDS             complement(<1..393)
                     /locus_tag="VF309_01075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7465202.1"
                     /translation="MFAHVDTPALILDAAKVERNCARMRERVHGAGVVLRPHVKTAKS
                     VEVAQLALGAPHGAITVSTLREAEYFFDHGFRDILYAVGMVPAKVRRVAALERRGARV
                     STIVDSVEAARALVEASRNADVRIPTMVE"
     gene            complement(401..1105)
                     /locus_tag="VF309_01080"
     CDS             complement(401..1105)
                     /locus_tag="VF309_01080"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7465203.1"
                     /translation="MPTASPQDFIALLEKGPAASACVFNPWRDCDERDRAPRHDMPRL
                     RRENMEAYIDARRTHARVMLLGEAPSHRGCRFTGIAFCSETELTQKADLVARRTLALT
                     SRDAEVKPQRERSAAVIWSEIERAGKPFEVVLWNAFPWHPYLAEGGESKDGPCGPSSN
                     RKPRSTEVAQGRAVMEALLGCFTQRLKVFAVGKVAEDALTRWESVECAGYIRHPAQGG
                     EALFRAQFRSKVAARL"
     gene            1147..1797
                     /locus_tag="VF309_01085"
     CDS             1147..1797
                     /locus_tag="VF309_01085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7465204.1"
                     /translation="MRRFALLMLGFCAALALAQSAEYRNEAAAAYRTILAGLAAKGKL
                     DDDGALLARVRRISPGLIAAAAQMRAETAAWSWEMHVTSDPAKGTFCMAGGKVLVGAG
                     LVKRLALDDGELAMLLGHEIAHAVADHRRAPSRLTMESDATEERREAALALAQEEEAD
                     RIGMQLAHRAGWPAAKLATFFDKLVAQESAGTFSASHPTAAARAAAAQVVARTLGD"
BASE COUNT          300 a          673 c          663 g          273 t
ORIGIN      
        1 ttccaccatc gtcgggatgc gcacgtccgc gttgcggctt gcctcgacca gcgcgcgcgc
       61 cgcctccacg ctgtccacga tcgtgctcac gcgcgcgccg cgccgctcga gcgcggccac
      121 gcgccgcacc ttcgcgggca ccatgccgac cgcatagaga atgtcgcgga agccgtggtc
      181 gaagaaatac tcggcctcgc gcagcgtcga taccgtgatc gcgccgtgcg gcgcgccgag
      241 agcgagctgc gccacttcca cgctcttggc ggtcttcacg tgcgggcgca gcaccacgcc
      301 cgcgccgtgc acgcgctcgc gcatgcgcgc gcaattgcgc tccaccttgg cggcgtcgag
      361 gatcagggcc ggcgtgtcga cgtgggcgaa catcgcgaaa ctatagacga gccgcgacct
      421 tgctccggaa ctgcgcgcgg aaaagcgcct cgccgccctg cgccggatgg cggatgtagc
      481 ccgcgcattc gaccgactcc cagcgcgtga gcgcatcctc ggcgaccttg ccgaccgcga
      541 acaccttcag gcgctgcgtg aagcagccga gcagcgcttc catcaccgcg cgcccctgcg
      601 cgacctcggt ggagcgcggc ttgcgattgc tcgaggggcc acacggtccg tccttggact
      661 cgccgccttc cgcaagatag ggatgccagg gaaaggcatt ccacagcacg acctcgaagg
      721 gcttgccggc gcgctcgatc tcgctccaga tcaccgcggc gctcctctcg cgctgcggct
      781 tcacctccgc gtcgcggctc gtcaacgcga gagtgcggcg cgcgaccaga tcggccttct
      841 gcgtgagctc ggtctcggag cagaacgcga tgccggtgaa gcggcagccg cggtggctgg
      901 gcgcctcccc gagcagcatc acgcgcgcgt gggttcgccg cgcatcgatg taggcctcca
      961 tgttctcgcg gcgcaggcgc ggcatgtcgt ggcgtggcgc gcggtcgcgc tcgtcgcaat
     1021 cgcgccacgg attgaagacg caggcagagg cggcggggcc cttttcgaga agggcgatga
     1081 agtcctgcgg ggaagcggtg ggcaccacgc attatagaat caggccccga tcgtcgagac
     1141 cacgaaatgc gccgcttcgc gcttctcatg ctgggtttct gcgcggcgct ggcgttggcc
     1201 cagtccgcgg agtaccgcaa cgaggccgcg gccgcctacc ggacgatcct cgcgggcctc
     1261 gcggccaagg ggaagctgga cgacgacggc gcgctcctcg cccgggtgcg ccgcatctct
     1321 ccggggttga tcgccgccgc ggcgcaaatg cgcgcggaaa ctgccgcgtg gtcgtgggaa
     1381 atgcacgtga cgagcgatcc cgcgaaggga acgttctgca tggcgggcgg caaggtgctg
     1441 gtgggcgccg ggctggtgaa gcgcctcgca ctcgacgatg gcgagctcgc gatgctgctc
     1501 ggccacgaaa tcgcgcacgc ggtggcggac catcgccgcg cgccgtcgcg gctcaccatg
     1561 gaatcggacg ccaccgagga gaggcgcgag gccgcgctcg cgctcgcgca ggaagaggag
     1621 gccgaccgca tcggcatgca gctcgcgcat cgcgccggat ggcccgccgc gaaactggcg
     1681 accttcttcg acaagctggt ggcgcaggaa tccgcgggca cgttcagcgc gagccatccc
     1741 accgccgcgg cgcgcgcagc ggccgcgcaa gtcgtggccc gcacgctcgg cgactagcga
     1801 tgcgaggccc catgaaaaat gcccggaagg ctgatcgtct tccgggcaaa aggccaccaa
     1861 caactttggc cggggaggct ccctagaact ttatgcgcaa gccgagtcc
//
LOCUS       DASVII010000084         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_615250,
            whole genome shotgun sequence.
ACCESSION   DASVII010000084 DASVII010000000
VERSION     DASVII010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615250"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VF309_01090"
     CDS             complement(<1..1078)
                     /locus_tag="VF309_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7465205.1"
                     /translation="MNTRLLNPVALLLALLHAYIGLRLLAPFGALAQAAGTAALLACL
                     WLLPKGFRMRETLGPIATLVPWIAMGFFSWLLVLTLAREASLIVAALALPAQANDWWA
                     RISAIGVMALTPLITLAGYVMARRVAPVVRVRVPLAGLPPELEGFTIAQISDIHVGPT
                     IKRAFVEAIVQRVNRLEADLVAITGDLVDGSVLDLAQHTQPLADLESRHGTYFVTGNH
                     EYYSGAHSWVHELRRLGARVLMNEHVVLDHDGAALAIAGVTDYSAHHFDASHRSDPHA
                     AVHGAPDDAVKVLLAHQPRSAESAEKAGYHLQLSGHTHGGQFWPWNLFVRLQQPFTAG
                     LNRLGGMWVYINRGTGYWGPPMRFG"
     gene            1225..1689
                     /locus_tag="VF309_01095"
     CDS             1225..1689
                     /locus_tag="VF309_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7465206.1"
                     /translation="MVSLLVNGKAVKVDAAPETPLLWALRDHLGMTGTKFGCGAGLCG
                     SCAVHVDGVPKLSCVVLLSEVAGKKVTTIEGLDGRAAKAVQAAWDKIQVPQCGYCQSG
                     QVMVATALLSKTPRPSDAQIDEAMQGNLCRCGTYGRIRAAIKDAAKQLEGSA"
     gene            1686..3821
                     /locus_tag="VF309_01100"
     CDS             1686..3821
                     /locus_tag="VF309_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7465207.1"
                     /translation="MNTVASVSRRNFLKVSAIAGGGMMISFRFAGAAEPAAAGSNAVT
                     ANAWVKLLPDGGVVLTCHRNEMGQDVHTSLAMLLAEELGVDPRRVRIEQAGVDPVYIN
                     RLLGGQITGGSTSVRDAWEPLRQAGASARMMLVSAAAEQWKVPASEVRAEDGHVTHGK
                     RKVAYGKLAAAAAKQPVPKDVKLKSKGEFKVIGSRLQRLDGADKARGRTVYGIDAKQP
                     GMLYAALAACPVIGGKVASFDGAAAEKRSGVRKVLDIGDGVAVVADHYWVARAALGDV
                     KIQWDEGPRAKLDTAAVYAALDRAKDRPGYMARNVGDVQGALSKGTQVEATYQCQMLS
                     HATMEPQNCLAKVSADGVDVWASVQFPQGAQGAAAEASGMKAEQVRIHPQFIGGGFGR
                     RLENDFVAQAVTIAKAMPGTPVKLIWIRPDDMQHDFYRPPSLHVMRGSVENGRLLAFS
                     SKLVSQSVTARAFPGFVKDGNDPFMTEGLANFTYDIPNCQLATVIEDVGVRVGYWRSV
                     SHALNSFATESFIDELALAAKVDPLAFRLGMLDKMPLQRAVIERAGQVSGYSAAPAKG
                     RAFGVAAMECYDTYAAVVCEVSASADKVKLERITIVADCGLMVHPDQAVAQLEGGVVT
                     GLISTLRSKITFKDGRVLQSNFHDFQLPRMKDVPPIKVEIIAQRDKPGGLGEVGVPLV
                     APAVANAVFALTGKRIRSLPLEDGGIAFA"
     gene            3978..4373
                     /locus_tag="VF309_01105"
     CDS             3978..4373
                     /locus_tag="VF309_01105"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7465208.1"
                     /translation="MFHDRDDERGEHRMRGNHQELTLTGASEVPAVTTSASGSGWVMI
                     HPDHSVTAKITTTGMTPTASHIHMAAAGANGPVIVPFTKEGDNTFVAPPGAKLSDEQY
                     EALKAGKLYVNVHSARNPGGEIRAQLKGW"
BASE COUNT          765 a         1452 c         1575 g          651 t
ORIGIN      
        1 cgccgaagcg catcggcggg ccccagtagc ccgtgccgcg attgatgtag acccacatgc
       61 cgccgagcct gttgagcccc gcggtgaacg gctgctgaag gcgcacgaag aggttccacg
      121 gccagaactg gccgccgtgc gtgtgccccg agagttgcag gtgatagccg gccttctcgg
      181 cggactcggc cgagcgcggc tgatgggcga ggagcacctt gacggcatcg tccggcgcgc
      241 catggacggc cgcgtgcggg tcgctccggt gcgaagcgtc gaaatggtgc gccgagtagt
      301 cggtgactcc cgcgatggcg agcgcggcgc cgtcgtgatc cagcacgaca tgctcgttca
      361 tcaggacgcg cgcgcccagg cgccgcagct cgtggaccca ggagtgcgcg ccggaatagt
      421 actcgtggtt cccggtgacg aaataggtcc cgtggcgcga ttcgagatcc gccagcggct
      481 gcgtgtgctg cgcgagatcg agcacgctgc cgtccaccag gtcgccagtg atcgcgacca
      541 ggtcggcctc gagccggttc acgcgctgca cgatcgcctc gacgaaggcg cgcttgatgg
      601 tgggccccac gtggatgtcg ctgatctgcg cgatggtgaa gccttcgagc tcgggaggaa
      661 ggccggcgag cggcaccctc actcgcacga cgggcgccac gcggcgcgcc atcacgtatc
      721 ccgccagggt gatcaacggg gtgagcgcca tgactccgat cgcggagatg cgcgcccacc
      781 aatcgttggc ttgcgcgggg agcgcgagcg ccgccacgat gaggctcgcc tcgcgggcga
      841 gagtcagcac caggagccac gagaagaatc ccatcgcgat ccacggaacg agcgtggcga
      901 tcgggcccag cgtctcgcgc atcctgaagc ccttgggaag gagccacagg catgcaagga
      961 gcgcggcggt ccccgcggcc tgcgcgagcg cgccgaatgg agccaggagc cgcaggccga
     1021 tgtaggcgtg gagcagggcg aggagcaatg cgacgggatt caggaggcga gtgttcatca
     1081 ggccgaagtg gggccgaacg gggtcgttct caaggactcg ccgccttccg gccgccgtcg
     1141 ccggcggcgg acgcacccct ggttgacagc gggccgggcg aggatcaacg tgcgctgata
     1201 ataattcccc cacggagaaa cgccatggtc agcctcctcg tcaacggcaa ggccgtaaag
     1261 gtcgatgccg ctcccgaaac tccgctccta tgggccctgc gggatcacct cggcatgacg
     1321 ggcaccaagt tcggttgcgg cgcgggccta tgcggctcgt gcgccgtcca tgtagacggg
     1381 gtgccgaagc tttcgtgcgt ggtgctgctc tcggaggtcg cgggcaagaa ggtgacgacg
     1441 atcgagggac tcgacggccg cgccgccaag gcggtccagg cggcatggga caagatccag
     1501 gttccccaat gcgggtactg ccaatcgggc caggtgatgg tcgcgacggc gctgctgtcg
     1561 aagacgccgc gtccgagcga tgcgcagatc gacgaagcga tgcagggaaa tctctgccgc
     1621 tgcgggactt acgggcgcat tcgcgccgcc atcaaggatg ccgccaagca actggagggc
     1681 tccgcatgaa taccgtcgcc tcggtctcgc gccgcaattt cctgaaggtc tccgccatcg
     1741 cgggcggtgg aatgatgatc tcgtttcgct tcgccggggc cgccgagccc gcggccgcgg
     1801 ggtcaaatgc cgtcacggcc aacgcctggg tcaagctcct tcccgatggc ggcgtggtgc
     1861 tcacgtgcca tcgcaacgag atggggcagg atgtgcacac ctcgctcgcg atgctgctcg
     1921 ccgaggagct cggcgtcgat ccgcggcgcg tgcgcatcga gcaggcgggc gtggaccccg
     1981 tgtatatcaa caggttgctg ggcggccaga tcaccggcgg cagcacgagc gtgcgcgatg
     2041 cgtgggagcc gttgcgccag gcgggcgcga gcgcccgcat gatgctggtg agcgccgcgg
     2101 cggagcagtg gaaggtcccg gcctcggaag tgcgcgcgga agacggccat gtcacccatg
     2161 gaaagcgcaa ggtggcctat ggcaagctcg ccgccgccgc ggcgaagcag cccgttccca
     2221 aggacgtgaa gctcaagtcc aagggcgaat tcaaggtcat cggaagccgc ctgcagcgcc
     2281 tcgacggcgc cgacaaggcg cgcggccgca cggtctacgg catcgacgcg aagcagcccg
     2341 gcatgctcta cgcggcgctc gccgcgtgcc cggtgatcgg cggaaaggtc gcgtcgttcg
     2401 acggcgccgc cgcggagaag cgctccggcg ttcgcaaggt cctcgacatc ggagacggcg
     2461 tcgcggtcgt cgccgaccac tactgggtcg cgcgcgcggc gctcggcgac gtgaagatcc
     2521 agtgggacga gggcccccgc gcgaagctcg atacggcggc ggtctacgcg gcgctcgatc
     2581 gcgccaagga ccggcccggc tacatggcga ggaacgtcgg cgacgtgcag ggcgcgctct
     2641 ccaaaggcac ccaggtcgag gcgacctacc agtgccagat gctctcccac gcgacgatgg
     2701 agccgcagaa ttgcctcgcg aaggtttccg cggacggcgt ggacgtctgg gcgagcgtgc
     2761 agtttccgca gggcgcgcag ggcgccgcgg cggaggcttc gggaatgaag gccgagcagg
     2821 tgcgcatcca tccgcagttc atcggcgggg gattcgggcg ccgcctggag aacgacttcg
     2881 tcgcgcaggc cgtgacgatc gcgaaggcga tgccgggcac gccggtgaag ctcatctgga
     2941 tccggcccga cgacatgcag cacgatttct atcgcccgcc gagcctgcac gtgatgcggg
     3001 gctccgtcga gaatggacgt ctcctagcat tctcctcgaa gctcgtctcg caatcggtca
     3061 ccgcgcgcgc gttccccggg ttcgtgaagg acggcaacga tcccttcatg acggaaggcc
     3121 tcgccaactt cacctacgac atcccgaact gccagctcgc caccgtcatc gaggacgtcg
     3181 gcgtgcgcgt cggctattgg cgctccgtga gccacgcgct caactcgttc gcgaccgaga
     3241 gcttcatcga cgagctcgcg ctcgccgcga aggtggatcc gctcgcgttc cgtctcggga
     3301 tgctcgacaa gatgccgctg cagcgcgccg tgatcgagcg cgcggggcag gtttcgggct
     3361 attccgccgc acccgcgaag gggcgcgcgt tcggcgtcgc ggccatggag tgctatgaca
     3421 cgtacgccgc ggtcgtctgc gaggtctccg caagcgcgga caaggtgaag ctcgagcgca
     3481 tcacgatcgt ggccgattgc ggcctcatgg ttcaccccga ccaggcggtg gcgcagctcg
     3541 aaggcggcgt cgtgacgggg ctcatcagca cgctgcgaag caagatcacc ttcaaggacg
     3601 ggcgcgtgct gcagtcgaac ttccacgatt tccagctgcc ccgcatgaag gacgtcccgc
     3661 cgatcaaggt cgagatcatc gcccagcgcg acaagccggg agggctcggc gaagtcggcg
     3721 tgccgctggt cgcgccggcc gtcgcgaacg cggttttcgc gctcacgggc aagcggatac
     3781 gttcgctacc gctcgaagac ggaggaatag ccttcgcctg attgaacttt tcccgtccgc
     3841 gccactccga cacgcgcctg gggcaattcc atggcgtcaa ctggacaacg gaggggatca
     3901 tgagcaaagc gacgagaagt acctggcggg cgggcgcagt tgcgttcgcc acggcgattg
     3961 ccttggcggg ctgcggcatg ttccacgatc gggacgacga gcgtggcgag caccgcatgc
     4021 gcggcaatca tcaggagctg acgctcaccg gcgcgagcga ggtcccggcg gtgacgacct
     4081 ctgcgagcgg cagcggctgg gtgatgatcc accccgatca cagcgtgacc gcgaagatca
     4141 ccactaccgg aatgacgccg acggcctctc acatccacat ggccgccgcg ggggccaacg
     4201 gaccggtgat cgtgcccttc accaaggaag gcgacaacac cttcgtcgcg ccgcccggcg
     4261 cgaagctctc cgatgagcaa tacgaagcgc tcaaggccgg caagctctac gtgaacgtgc
     4321 acagcgccag gaaccccggc ggcgagattc gcgcgcagct gaagggctgg taacggtcca
     4381 tcgcggcggt gaagacggcg gctcgcgggc cgccgttttt catgtagatc ccgcggccgg
     4441 cgg
//
LOCUS       DASVII010000085         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_327946,
            whole genome shotgun sequence.
ACCESSION   DASVII010000085 DASVII010000000
VERSION     DASVII010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327946"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VF309_01110"
     CDS             <1..338
                     /locus_tag="VF309_01110"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7465209.1"
                     /translation="KNGWPMAIAIVDTSGQLVMFSRADNTQTASVSVAQDKAVSSAMY
                     RRPTKVMQDAVAGGGAGIRFLNLRGASTVEGGLPLYVDGRIVGAIGVSGMASDQDGVC
                     AKAGVDALK"
     gene            complement(331..963)
                     /locus_tag="VF309_01115"
     CDS             complement(331..963)
                     /locus_tag="VF309_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7465210.1"
                     /translation="MIPHDTGLAVDALLFDLGGVVMSLDWNRAFSRWAGDSGESAEAL
                     RGRFRFDAPYERHERGEIGERDYYASLREALRIELTDAQWDAGWGAIFAGEITPTVAA
                     IAKVKGRIPVYAFSNSNAAHQRVWSRRFGAALGHFRKVFVSSDLGKRKPERAAFEAIA
                     HAIGVPLDRILFFDDTMENVEGARAAGLRTVHVRTPQDVEDALRPWLEAT"
     gene            complement(950..1555)
                     /locus_tag="VF309_01120"
     CDS             complement(950..1555)
                     /locus_tag="VF309_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7465211.1"
                     /translation="MTPAYWSEAKKHLARRDPVLRKLMKKYPDAELGTRGDAFQTLAR
                     SITGQQISVKAAQSIWNRFTACAGTVNARTVVALAPEAIRACGFSGSKVAYVKDLAAR
                     FHGGAIKPRRWPKMDDEAIIEDLVQVKGIGRWSVEMFLIFHMMRPNVLPVGDLGLQRA
                     MERAYNAGELLSKAEMRAIGEKWAPWSTVATWYLWRSLDPA"
     gene            complement(1552..2049)
                     /locus_tag="VF309_01125"
     CDS             complement(1552..2049)
                     /locus_tag="VF309_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX7465212.1"
                     /translation="MNRARSAHGFTWIEMLAVMAVLAILALMAIPSMQDSALKQQVKD
                     GLALAAVAKAGVQAAYAASGKMPADNKAAGIPDHDKIVGTLVKDVLVDGGAITLTFGN
                     NANKAIADKKVTVRPAVVPGEALVPIAWLCHQTAIPPKMEVQGNDATDIPPKWLPIEC
                     RTAPQ"
BASE COUNT          359 a          746 c          763 g          354 t
ORIGIN      
        1 gcaagaacgg ctggccgatg gcgatcgcga tcgtcgatac ctcgggccag ctggtgatgt
       61 tctcgcgcgc ggacaacacg cagacggcga gcgtcagcgt cgcgcaggac aaggcggttt
      121 cgtcggcgat gtaccggcgc ccgacgaagg tgatgcagga cgcggtcgcg ggaggcgggg
      181 cggggattcg cttcctcaac ctgcgcggtg cgagcaccgt cgagggcgga ttgccgctct
      241 acgtcgacgg ccggatcgtc ggggcgatcg gcgtctcggg catggcttca gaccaggatg
      301 gcgtgtgcgc caaggccgga gtcgacgccc tcaagtagct tcgagccagg gccgcagggc
      361 gtcctcgacg tcctgcggag ttcgcacgtg cacggtccga agtcccgcgg cgcgcgcgcc
      421 ttccacgttt tccatcgtgt cgtcgaagaa gaggatgcga tccaggggga cgccgatcgc
      481 gtgcgcgatc gcctcgaagg ccgcgcgctc gggcttgcgc ttgccgagat ccgaggacac
      541 gaagaccttg cggaagtggc cgagcgccgc gccgaaccgg cgcgaccaca cccgctggtg
      601 cgcggcgttc gaattcgaga acgcgtagac cgggatgcgg cccttcacct tcgcgatcgc
      661 ggcgaccgtc ggcgtgatct cgccggcgaa gatcgcgccc cagcccgcgt cccactgggc
      721 gtcggtgagc tcgatgcgca aggcctcgcg cagcgacgcg tagtagtcgc gctcgccgat
      781 ctcgccgcgc tcgtggcgct cgtagggagc gtcgaaccgg aagcggccgc gcaacgcctc
      841 ggcactctct cccgaatcgc cggcccaacg cgagaacgcg cgattccagt cgagcgacat
      901 gacgacgccg cccaggtcga agagcagggc atcgacggca agcccggtgt catgcgggat
      961 caagggagcg ccacaggtac caggtggcga cggtgctcca cggtgcccac ttctcgccga
     1021 tcgcgcgcat ctcggctttg ctgaggagct cgcccgcgtt gtaggcgcgc tccatcgcgc
     1081 gctgcaggcc gaggtcgccg acgggcagca cgttgggccg catcatgtgg aagatgagga
     1141 acatctcgac gctccagcgc ccgatgccct tcacctggac gaggtcctcg atgatcgcct
     1201 cgtcatccat cttcggccag cggcgcggct tgatcgcccc cccatggaag cgcgcggcga
     1261 ggtccttcac gtaggcgacc ttgctgcccg agaagccgca ggcgcgaatc gcctcgggcg
     1321 cgagcgccac gacagtccgg gcattcaccg ttcccgcgca ggcggtgaag cggttccaga
     1381 tggattgcgc cgccttgacg gaaatctgct gtccggtgat cgaacgcgcg agcgtctgga
     1441 aggcgtcgcc gcgcgtcccc agctccgcgt cgggatactt cttcatgagc ttgcgcagca
     1501 ccggatcgcg ccgcgccagg tgcttcttcg cctcgctcca gtacgcgggc gtcattgcgg
     1561 tgccgtgcgg cactcgatcg gcagccactt cgggggaatg tcggtcgcgt cgtttccctg
     1621 cacttccatc ttgggaggaa ttgccgtctg gtggcacagc caggcgatcg gcacgagcgc
     1681 ttcgccgggc accacggcgg ggcgcaccgt gaccttcttg tcggcgatgg ccttgttcgc
     1741 gttgttgccg aaggtgagcg tgatcgcgcc gccgtcgacg aggacatcct ttaccagcgt
     1801 gcccacgatc ttgtcatggt cgggaatccc cgcggccttg ttgtccgcgg gcatcttgcc
     1861 cgaggccgca tacgcggcct gcacgccggc cttggccacg gcggcgagcg cgaggccatc
     1921 cttcacctgt tgcttgagcg cggagtcctg catcgaggga atcgccatga gggcgaggat
     1981 ggccagcacc gccatgacgg caagcatctc gatccacgtg aagccatggg cactgcgagc
     2041 gcgattcatg tgggccatga taccgaacgg atgaggcagc acgcggtcta ccccgaatcg
     2101 ccggagaaag atcatggtcc gagaaaagca cccgacgctc cccagaacgc gcccccccat
     2161 gccgcctccc ggcacgcccg agcacgagga atggctcatc gacgaggcgg gtgacgaaag
     2221 ct
//
LOCUS       DASVII010000086         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_219901,
            whole genome shotgun sequence.
ACCESSION   DASVII010000086 DASVII010000000
VERSION     DASVII010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219901"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VF309_01130"
     CDS             complement(<1..960)
                     /locus_tag="VF309_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7465213.1"
                     /translation="MRITYPRSFLSLLLIGFSIVAAPLIFALFTNALAFEKLAGLSEQ
                     AVHSAVKATQASRSLLAVIALLDRSARQYAIAGEATFLDAYRANRATFLGLTATLDSM
                     ALSDNQRSEVATIERREEDVNKLITRMGPTPELSRRLPREFASLADSGQALVKLGDQG
                     VDEGIEQLRTQAVESRNKVFWLMIALIPTAVVLIASFTFLISRPIRQVTESIRGLGDG
                     QFARPIRIEGPGDMVRLGAQLDWLRERLVTLEAQKTRFLQHMSHELKTPLTALREGSD
                     LLYSGVVGNLNAEQREIARILQENSIELRKLIEGLLNYSAVHAQ"
     gene            1024..1227
                     /locus_tag="VF309_01135"
     CDS             1024..1227
                     /locus_tag="VF309_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEX7465214.1"
                     /translation="MYESEATRFIRDFLKKNPQVVEQQRKNRATWWDKPQDLETTNEH
                     SESAVPQPPYVYFPLPTRDAGKP"
     gene            complement(1224..>1660)
                     /locus_tag="VF309_01140"
     CDS             complement(1224..>1660)
                     /locus_tag="VF309_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX7465215.1"
                     /translation="VYAERLRFNKGAERLLSAAKRRGVKTLLVSGGFTYFTDRVREAL
                     GFDYAYSNTLVLEGGKLAGSVTGPLVDARGKAGHVARLKGELGLARERVLAIGDGAND
                     LLMMAEAGTSIAYHAKPVVRDKADYALDYAGLDGVLNLFPEA"
BASE COUNT          280 a          551 c          559 g          270 t
ORIGIN      
        1 ctgcgcgtgc acggcgctgt agttgagcag gccctcgatc agcttcctca gctcgatgga
       61 gttctcctgc aggatgcgcg cgatctcgcg ctgctcggcg ttcaggttgc cgaccacgcc
      121 cgaatagagc aggtccgagc cttcgcgcaa cgcggtgagc ggcgtcttca gctcgtggga
      181 catgtgctgc aggaagcgcg tcttctgcgc ctcgagggtc acgaggcgct cgcgcagcca
      241 gtcgagctgc gcgccgaggc gcaccatgtc gcccggcccc tcgatgcgga tcggcctcgc
      301 gaactggcca tcgcccaggc cgcgaatgct ctcggtcacc tgccggatgg ggcgcgagat
      361 gaggaacgtg aacgaagcga tcagcaccac ggcggtcggg atgagggcga tcatcagcca
      421 gaagaccttg ttgcgcgatt cgaccgcctg ggtgcgaagc tgctcgatcc cctcgtcgac
      481 tccctggtcg ccgagcttca ccagggcctg gccgctgtcg gcgagcgagg cgaactcgcg
      541 cggcagcctg cgcgagagct cgggcgtggg tcccatgcgg gtgatcagct tgttgacgtc
      601 ctcttcccgc cgctcgatgg tcgcgacctc ggagcgctgg ttgtccgaaa gcgccatcga
      661 atcgagcgtg gccgtgaggc cgaggaatgt cgcgcgattg gcgcggtacg cgtcgaggaa
      721 tgtcgcctcg cccgcgatcg cgtactggcg cgccgagcga tcgagcaagg cgatgacggc
      781 cagcagggag cggctcgcct gcgtcgcctt caccgccgag tggaccgcct gctccgagag
      841 gccggcgagc ttctcgaacg cgagcgcgtt ggtgaagagc gcaaagatca gcggcgccgc
      901 gacgatcgag aagccgatca gcagcaggga caggaacgag cgcggatagg tgatgcgcat
      961 ctcaggcgat tataatgtcc gtgaatccgc cgcctcgcac gggatttctc gaaggaaggc
     1021 cccatgtacg aatccgaagc cacccgcttc atccgcgatt tcctgaagaa gaatccgcag
     1081 gtcgtggagc agcagaggaa gaaccgcgcg acctggtggg acaagccgca ggacctcgag
     1141 accacgaacg agcactcgga gtccgcggtg ccccagcccc cttacgtcta tttcccgctg
     1201 cccacgcgcg acgccggcaa gccctaggcc tccggaaaca ggttgagcac gccgtcgagg
     1261 cccgcgtaat ccagggcgta gtcggctttg tcccggacga cgggctttgc gtgatacgcg
     1321 atgctcgttc ccgcttccgc catcatgagc aggtcgttcg cgccgtcgcc gattgcgagg
     1381 acgcgttcgc gcgcgagtcc cagctcgccc ttcagccttg cgacgtgccc ggccttgccg
     1441 cgcgcgtcga cgaggggtcc cgtcaccgac cccgcgagct tgccgccctc caggaccagc
     1501 gtattcgaat aggcgtagtc gaagcccagc gcctcgcgca cgcgatcggt gaagtaggtg
     1561 aatccgccgg agacgaggag tgttttcaca ccgcggcgct tcgcggccga gagcagcctt
     1621 tccgcgccct tgttgaagcg caggcgctcc gcgtagacgc
//
LOCUS       DASVII010000087         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_219905,
            whole genome shotgun sequence.
ACCESSION   DASVII010000087 DASVII010000000
VERSION     DASVII010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219905"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VF309_01145"
     CDS             <1..386
                     /locus_tag="VF309_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX7465216.1"
                     /translation="YTLSQETGDTATIVKLLRVAMLLPVCLAIGLALHVRGAAAAHAA
                     PVLPWFAVVFAAFVAVTSIGWIPAPVIEAGSVVSRWCLVTAIAAIGMKTSLKSLVDMG
                     LKSVMLVVAETVFLAALVLGVLAWK"
     gene            383..1102
                     /locus_tag="VF309_01150"
     CDS             383..1102
                     /locus_tag="VF309_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7465217.1"
                     /translation="MNELRVNVWRGGADGRFQQFAVPRRASQTVLDVVTYIQRRLDPT
                     LAYRFACRVGMCGSCAMTVNGAPRWTCRTHVDRVARDGALEIAPLANMPVVRDLVADM
                     REFFDKWAKARGQFTPTRTRADEFAVVKPASRQRMEADAGIECIGCGVCYAACDVVKW
                     NRDYLGPAALNRAWTLANDARDGGQRARLLAVAGDAGCHSCHTTMSCTELCPKKLSPT
                     RSISGLKRATLAIALRAEGGK"
     gene            1099..1440
                     /locus_tag="VF309_01155"
     CDS             1099..1440
                     /locus_tag="VF309_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465218.1"
                     /translation="MSARHEARLWVVQRASAALLAFCVAVHLVTIVYAVRGGLSAAEV
                     LSRTRGNLAWMAFYALFVVAAAAHGSIGLRTVAMEWLGWRKSTADLLMWFAASGLAAL
                     GAQAVYAVVLP"
     gene            1437..1772
                     /locus_tag="VF309_01160"
     CDS             1437..1772
                     /locus_tag="VF309_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465219.1"
                     /translation="MKPARNHPAYWAFLVHRISGVALALFLPLHFFVLGRAIDGEAAL
                     DGFLAWSHRPMVIAAEWVLVTLLAAHLAGGLRLLALEFLPWRDWQKTLAALAAAFALL
                     AGLAFALAR"
     gene            1816..>2272
                     /locus_tag="VF309_01165"
     CDS             1816..>2272
                     /locus_tag="VF309_01165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7465220.1"
                     /translation="MKHRVSRIVLFALIAAASFVAAAQPWPSKPVKIIVPFAAGGTTD
                     VVARLLGQKLGDAWATSVIIEDRAGAGGNIGADAAAHSPPDGYTLFMTSGSIVTANQY
                     MYKSLTYDAARDLAPITGVATGPQVVVVNPSSPFKNLREFIAYAKANPRK"
BASE COUNT          299 a          786 c          797 g          390 t
ORIGIN      
        1 gctacaccct ttcccaggaa acgggcgata ccgcgacgat cgtaaagctc ctgcgcgtcg
       61 cgatgctgct cccggtgtgc ctcgcgatcg gcctcgccct tcacgtgcgc ggcgccgccg
      121 cggcgcacgc ggcgccggtg ctgccgtggt tcgcggtggt gttcgccgcg ttcgtcgccg
      181 tgacgagcat cggatggatt ccggcgccgg tcatcgaagc gggctccgtc gtctcgcgct
      241 ggtgcctggt gaccgccatc gccgcgatcg gcatgaagac atcgctcaag tcgctggtgg
      301 acatggggtt gaagtccgtg atgctggtcg tggccgagac cgtgttcctc gcggcgctcg
      361 tcctgggagt tctcgcgtgg aagtgaacga gctacgcgtg aacgtttggc gcggcggggc
      421 ggacggacgc ttccagcagt tcgccgtccc gcggcgcgcg agccagaccg tgctcgacgt
      481 ggtgacctac atccagcgcc gcctcgaccc cacgctcgcc tatcgcttcg cctgccgcgt
      541 cggcatgtgc ggctcgtgcg cgatgacggt gaacggggcg ccgcgctgga cgtgccgcac
      601 gcacgtcgac cgggtggcgc gcgacggggc gctcgagatc gcgccgctcg ccaacatgcc
      661 cgtggtgcgc gacctcgtcg ccgacatgcg cgagttcttt gacaagtggg cgaaggcgag
      721 gggacagttc acgcctaccc gcacgcgcgc cgacgagttc gccgtcgtga agcccgcttc
      781 gcggcagcgc atggaagccg atgccggaat cgagtgcatc ggctgcggcg tctgctacgc
      841 ggcctgcgac gtcgtgaagt ggaaccgcga ctacctgggg cccgcggcat tgaatcgcgc
      901 gtggacgctc gcgaacgacg cgcgcgacgg cgggcagcgg gcgcgccttc tcgcggtcgc
      961 gggcgacgcg ggctgtcact cctgccacac gacgatgagt tgcaccgagc tatgccccaa
     1021 gaagctctcg ccgacgcgct cgatctccgg gctgaagcgc gcgacgctcg cgatcgcctt
     1081 gcgcgccgag ggcggcaaat gagcgcgcgg cacgaggcgc ggctgtgggt cgtgcagcgc
     1141 gcgagcgccg ccctgctcgc gttctgcgtc gcggtgcacc tggtcacgat cgtctatgcg
     1201 gtgcgcggcg ggctttcggc agccgaggtg ctgtcgcgca cgcgcggaaa cctcgcgtgg
     1261 atggcgttct acgcgctctt cgtcgtggcc gccgcggcac acgggtcgat cggtcttcgc
     1321 accgtcgcga tggagtggct cggctggagg aaatccacgg ccgacctcct gatgtggttc
     1381 gcggcgagcg gcctcgcggc tctcggagcg caggcggtgt acgcggtggt gctcccgtga
     1441 agcccgcgcg caaccatccg gcgtactggg cgttcctcgt gcatcgcatc tcgggagttg
     1501 cgctggcgct cttcctgccg ctgcacttct tcgtgctcgg gcgcgccatc gacggcgaag
     1561 ccgcgctcga cgggttcctc gcctggagcc accggccgat ggtgatcgcc gccgagtggg
     1621 tcctcgtcac gctcctcgcc gcccatctcg cgggaggctt gaggctcctc gcgctggaat
     1681 tcctgccgtg gcgcgactgg cagaagaccc tcgcggcgct ggccgccgcc ttcgccctcc
     1741 tcgccgggct cgccttcgcg ctcgcgcgtt gagcgctatg cttccgtgat cccctctatt
     1801 caggagaagc cgcagatgaa gcaccgcgtc tcgcgcatcg tccttttcgc gttgatcgcc
     1861 gcggcgtcgt tcgtcgcggc ggcgcagccg tggccctcga agcccgtgaa gatcatcgtg
     1921 ccgttcgcgg ccggcggcac gacggacgtg gtggcgcggc tcctcgggca gaagctcggc
     1981 gatgcctggg caacgtcggt catcatcgag gatcgcgccg gtgccggagg caacattggc
     2041 gcggacgccg cggcgcattc gccgcccgat ggctatacat tgttcatgac gtccggctcg
     2101 atcgtcaccg cgaatcaata catgtacaag tcgctcacct acgatgccgc gcgcgacctc
     2161 gccccgatca cgggcgtggc gacagggccc caggtggtcg tggtgaaccc atcctcgccg
     2221 ttcaagaacc tgcgcgagtt catcgcctac gcgaaggcca acccgaggaa gg
//
LOCUS       DASVII010000088         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_435857,
            whole genome shotgun sequence.
ACCESSION   DASVII010000088 DASVII010000000
VERSION     DASVII010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435857"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="accD"
                     /locus_tag="VF309_01170"
     CDS             complement(<1..740)
                     /gene="accD"
                     /locus_tag="VF309_01170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7465221.1"
                     /translation="MSWFRKLLPPKIKREGTQQKKAVPEGLWSKCPSCEAVLYFTDLE
                     NNLHVCPKCGFHNRLSARQRLDLFLDSEGRAELASEVIPLDPLKFKDSRKYSERLEEA
                     EKETGETDALVVMQGSVKSVPVVVAAFEFGFLGGSMGSVVGERFVRAVQACCDEGLPF
                     ICFTATGGARMQEGLLSLMQMAKTTAALHQLTAARQPFISILTDPTMGGVSASFAFIG
                     DVVIAEPGALIGFAGPRVIEQTVRETLP"
     gene            complement(737..1546)
                     /gene="trpA"
                     /locus_tag="VF309_01175"
     CDS             complement(737..1546)
                     /gene="trpA"
                     /locus_tag="VF309_01175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7465222.1"
                     /translation="MSRIDATFARLRTERRAALIPFVTAGDPSPQQTVPVMHALVKGG
                     ADVIELGVPFSDPMADGPVIQRSSERALKHGVGLRDVLASVAEFRRANGETPVVLMGY
                     ANPIERMGLAAFVAKARDAGVDGVLIVDYPPEESADWLAALDGSGIDPIFLLSPTSSE
                     ARIELVSRVARGYIYYVSLKGVTGAANIDTREVEAMVKRIRARSAVPVGVGFGIRDGA
                     TARQVARIADAVVIGSRIVQEMNDAPNDAVARAEAVLREFRGAMDAAEALA"
BASE COUNT          260 a          539 c          522 g          240 t
ORIGIN      
        1 tcgggaaggg tctcgcgcac cgtctgctcg atgacgcgcg ggcccgcgaa accgatgagc
       61 gcccccggct cggcgatcac gacatcgccg atgaacgcga agctcgccga gacgccgccc
      121 atggtgggat cggtgaggat cgagatgaag ggctggcgcg cggcggtgag ctggtgcaac
      181 gcggcagtgg tcttcgccat ctgcatgagc gacaggagtc cctcctgcat gcgcgccccg
      241 cccgtggcgg tgaagcagat gaaaggcaat ccctcgtcgc agcaggcctg caccgcgcgc
      301 acgaagcgct cgcccaccac cgaacccatc gatccgccaa gaaagccgaa ttcgaacgcg
      361 gccacgacca ctggaacact cttgacggac ccctgcatca ccaccagcgc gtcggtctcg
      421 ccggtttcct tctccgcttc ctcgagccgc tcgctgtact tgcggctgtc cttgaacttg
      481 agggggtcga gcggaatcac ctcggatgcg agctccgcgc gcccttcgga atcgaggaag
      541 aggtcgaggc gctggcgcgc cgacaggcga ttgtggaagc cgcacttcgg gcagacgtgc
      601 aggttgttct cgaggtcagt gaaatagagc accgcctcgc acgacgggca cttgctccac
      661 aggccctcgg ggacggcctt cttctgctgc gtgccctcgc gcttgatctt gggcggcagg
      721 agcttgcgga accagctcaa gcgagagcct cggcggcatc catcgcgccg cggaattcgc
      781 gcagcactgc ctcggcgcgc gccaccgcgt cgttcggcgc gtcgttcatc tcctggacga
      841 tgcggctgcc gatgacgacc gcgtccgcga tgcgggcgac ctgccgcgcg gtcgcgccgt
      901 cgcgaatgcc gaagcccacg ccgaccggca ccgcgctgcg cgcgcgaatg cgtttgacca
      961 tcgcctcgac ctcgcgcgtg tcgatgttcg cggcgccggt cactcccttc aacgagacat
     1021 agtagatgta gccgcgtgcc acccgggaaa cgagctcgat gcgcgcttcg ctcgaagtgg
     1081 gcgacaggag aaagatcgga tcgatgcccg agccgtcgag cgccgcgagc cagtccgcgc
     1141 tctcctccgg aggatagtcg acgatgagca cgccatcgac gccggcgtcg cgcgccttgg
     1201 cgacgaacgc ggcgaggccc atgcgctcga tcggattcgc gtagcccatc agcacgaccg
     1261 gggtctcgcc gttcgcgcgg cggaattcgg cgacgctcgc gagcacgtcg cgcaatccga
     1321 caccgtgctt cagcgcgcgc tccgaggagc gctggatcac gggaccgtcc gccatcgggt
     1381 ccgagaacgg cacgccaagc tcgatcacat cggctccgcc cttcaccaga gcgtgcatca
     1441 ccgggaccgt ttgctgcggg ctcggatcgc ctgcggtgac gaacggaatg agcgcggcgc
     1501 gccgttcggt gcgtagacgg gcgaaggtgg cgtcgatgcg gctcacgggc gctctcagaa
     1561 c
//
LOCUS       DASVII010000089         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_615507,
            whole genome shotgun sequence.
ACCESSION   DASVII010000089 DASVII010000000
VERSION     DASVII010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615507"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF309_01180"
     CDS             <1..457
                     /locus_tag="VF309_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7465223.1"
                     /translation="PTWEYPVADWKQVLDINLTGVFLCNKRAVPHMRENDYGRIVNIA
                     SIAGKEGNPNASAYSASKAGVISLTKSLGKEVAKTGIRVNCVTPAAVKTGMFAQMTQA
                     HIDFMLSKIPMGRFGQVEEIAALVAWLCSEECSFSTGAVFDLSGGRAVY"
     gene            470..928
                     /locus_tag="VF309_01185"
     CDS             470..928
                     /locus_tag="VF309_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465224.1"
                     /translation="MRPTLPFLFASLAVSATAWATPPDACTILTADELNTIAGGTVAS
                     VQQRKSGNPSECDYIDKRKGAVLVVSIREVQYAAENELQYERENLEKIYRGKVKYLTG
                     VGENGFWMPVNKQLVFRKAKTLVSVTFSRVANQNEADSSQVARMIESRLK"
     gene            928..>1999
                     /locus_tag="VF309_01190"
     CDS             928..>1999
                     /locus_tag="VF309_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX7465225.1"
                     /translation="MRLSPGGLTLAQLASFAKSHDALALDDASRERLRAGAEAVRRIV
                     AQGQPVYGINTGFGKLAQTHIPDDQLELLQRNLVLSHSVGVGALLADDVVRLTMLLKV
                     ASLARGYSGVRVEVVEAILALLNAGIYPCIPSKGSVGASGDLAPLAHMSAALLGIGSV
                     RHRGEVIAAAEGLKRAGLAPLRLGPKEGLALLNGTQVSTALALRGLFSAQSVFAAAVV
                     AGAMSVDAAMGSDTPFDARIHALRGHAGQIEAAAMYRALLAGSEIRASHLTGDDKVQD
                     PYSLRCQPQVMGACLDVMNHAARTLLIEANGVTDNPLVFAQTDESLSGGNFHAEPVAF
                     AADHLALAIAEIGALSERRIALL"
BASE COUNT          333 a          695 c          660 g          311 t
ORIGIN      
        1 gccgacgtgg gagtatcccg tcgccgactg gaaacaggtt ctcgacatca acctcaccgg
       61 cgtcttcctc tgcaacaaga gggccgttcc ccacatgcgg gaaaacgact acgggcgcat
      121 cgtgaacatc gcctccatcg cgggcaagga gggcaatccc aacgcgtcgg cctacagcgc
      181 ttcgaaggcg ggcgtgatct ccctcaccaa gtccctcggc aaggaagtcg cgaagaccgg
      241 catccgcgtg aattgcgtga cgccggcggc ggtgaagacc ggcatgttcg cgcagatgac
      301 ccaggcgcac atcgacttca tgctctcgaa gatccccatg gggcgcttcg gccaggtgga
      361 ggagatcgcc gccctcgtcg cgtggctctg cagcgaggaa tgctccttct cgacgggcgc
      421 cgtgttcgac ctctcgggcg ggcgcgctgt atattgacga catccaacga tgaggcccac
      481 tcttcccttc cttttcgcgt cgctggccgt ctcggcgact gcctgggcga cgccgcccga
      541 cgcatgcacg atcctcaccg ccgacgagct caacaccatc gcgggaggaa ccgtggcctc
      601 cgtgcagcag cgcaagtccg gcaatcccag cgaatgcgac tacatcgaca agcgcaaggg
      661 cgcggtgctg gtggtgtcga tacgcgaggt gcagtacgcg gccgagaacg agctgcaata
      721 cgagcgcgag aacctcgaga agatctaccg tggcaaggtg aagtacctga ccggcgtcgg
      781 cgagaacggc ttctggatgc cggtcaacaa gcaactcgtg ttccgcaagg cgaagaccct
      841 cgtttcggtg accttttccc gcgtggccaa ccagaacgag gccgattcct cgcaggtcgc
      901 ccgcatgatc gagtcgcgcc tcaaatagtg aggctctcgc ccggcgggct cacgctcgcg
      961 cagctcgcat cgttcgcgaa gtcccacgac gctcttgctc tggacgacgc gagccgcgag
     1021 cgcctgcgcg ccggcgccga ggccgtgcgc cgcatcgtcg cccaggggca gccggtctac
     1081 ggaatcaaca cgggcttcgg caagctcgcg cagacgcata ttcccgacga ccagctcgag
     1141 ctcctgcaga ggaacctggt cctgtcgcac agcgtgggcg tgggcgcgtt gctcgccgac
     1201 gacgtggtgc gcctcacgat gctcctcaag gtcgcgagcc tcgcccgcgg gtactcgggc
     1261 gtgagagtcg aggtggtcga ggcgatcctc gcgctgctga acgccgggat ctacccgtgc
     1321 attccgtcga agggatccgt gggcgcctcg ggcgacctcg cgccgctcgc gcacatgagc
     1381 gcggcgctcc tcgggatcgg cagcgtgcgc catcgcggcg aagtgattgc ggcggccgag
     1441 ggattgaagc gtgcgggcct tgcgccgctg aggctcggcc ccaaggaagg cctcgccctc
     1501 ctgaacggca cccaggtctc gaccgcgctc gcgctgcgcg gcctcttctc ggcgcagagc
     1561 gtcttcgccg ccgcggtggt cgcgggcgcg atgtcggtgg acgccgcgat gggcagcgac
     1621 acgcccttcg acgcgcgcat ccacgcgctg cgcgggcacg ccgggcagat cgaggcggcg
     1681 gcgatgtacc gcgcgctcct cgccgggagc gaaatccgcg cctcgcacct caccggagac
     1741 gacaaggtgc aggaccccta cagcctgcgc tgccagccgc aggtgatggg cgcgtgcctc
     1801 gacgtgatga accacgccgc gcgcacgctc ctcatcgagg cgaacggcgt caccgacaac
     1861 ccgctcgtct tcgcgcaaac cgacgagtcg ctttcgggcg gcaacttcca cgcggagccg
     1921 gtcgctttcg ccgccgatca tctcgcgctc gccatcgcgg agatcggggc cctgtcggag
     1981 cggcgcatcg ccttgctga
//
LOCUS       DASVII010000090         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_328298,
            whole genome shotgun sequence.
ACCESSION   DASVII010000090 DASVII010000000
VERSION     DASVII010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328298"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VF309_01195"
     CDS             <1..374
                     /locus_tag="VF309_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465226.1"
                     /translation="LHEGKVYNNLHWVTDGVDALDFLKQRGKHAGAPRPDIILLDLNL
                     PKKDGREVLSEIKQDADLRAIPVVILTTSQAEEDVLRSYDLHANCYVTKPVDLEKFIL
                     VVQSIDRFWLNVVTLPPSNGR"
     gene            371..>1773
                     /locus_tag="VF309_01200"
     CDS             371..>1773
                     /locus_tag="VF309_01200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7465227.1"
                     /translation="MKTEAPGHSVLLIEDNPGDARLIREMLAEEPAAPFRMRHADRLS
                     RGLDALAQKDVDLVLLDLSLPDSHGLDTFSKVYAHSPKVPIIVLSGNDDQQLALYAVK
                     SGAQDYLVKGKIDRELLLKAMQYSIERKRYQEELERQANYDGLTGLPNRHLFHDRLKQ
                     AVFAQRSTKSIAVVFIDLDHFKVINDSLGHNFGDEVLRHVGERLTAAVRDGDTVARLG
                     GDEFVLILSDQTREEVIFRTMRRLIGKVGEPMIVGDRELNITCSAGISLYPQDGPDVQ
                     TLLKNADAAMYRAKSVGRNTFQFFTAEMNELANERLSMEQALRRAIERDELLLHYQPR
                     LNLLTGEVDGVEALVRWMHPVRGLTYPDRFISLAEETGLIVPIGEWVLRTACEQGRDW
                     RRAGFRPVISVNLSARQLWGGGLVRLVGDVIAETGMSEQLELELTESMVMHDAENVIA
                     TMLGLKALGVRLSVDDF"
BASE COUNT          318 a          601 c          580 g          274 t
ORIGIN      
        1 cgctgcacga gggcaaggtc tacaacaacc tgcattgggt gaccgacggc gtcgacgcgc
       61 tcgacttcct caagcaacgc ggcaagcatg caggagcgcc gcgtcccgac atcatcctgc
      121 tcgacctcaa ccttcccaag aaggacgggc gcgaggtgct ctcggagatc aagcaggacg
      181 ccgacctgcg cgcgatcccc gtggtgatcc tcaccacctc gcaagccgag gaggacgtgc
      241 tgaggagcta cgacctgcac gccaactgct acgtgaccaa gcccgtggac ctcgagaaat
      301 tcatcctggt ggtccagtcc atcgaccgct tctggctcaa cgtcgtgacg cttccgccca
      361 gcaacggccg atgaagaccg aggcccccgg ccattccgtc ctgctgatcg aggacaatcc
      421 cggcgacgcg cgcctcatcc gcgagatgct cgccgaggag ccggcggcgc ccttccgcat
      481 gcggcacgcc gatcggctct cgcgcggcct cgacgcgctc gcgcagaaag acgtcgacct
      541 cgtgctgctc gacctttcgc tgcccgacag ccacgggctc gacactttct ccaaggtcta
      601 cgcgcactcc ccgaaggtgc cgatcatcgt gctttccggc aacgacgacc agcagctcgc
      661 gctctacgcg gtgaagagcg gcgcgcagga ctacctggtg aagggcaaga tcgaccggga
      721 gctgctgctg aaggcgatgc agtattcgat cgagaggaag cgctaccagg aggagctcga
      781 gcgccaggcc aactacgacg ggctcacggg ccttcccaac cgccatcttt tccacgatcg
      841 cctgaagcag gcggtcttcg cgcagcgcag caccaagtcg atcgcggtgg tgttcatcga
      901 cctcgaccac ttcaaggtga tcaacgattc gctcggccac aacttcggcg acgaggtgct
      961 gcgccacgtc ggcgagcgcc tcacggcggc ggtgcgcgac ggcgacacgg tggcgcgcct
     1021 gggcggcgac gagttcgtgc tgatcctctc cgaccagacg cgcgaggaag tgatcttccg
     1081 caccatgcgc cgcctcatcg gcaaggtggg ggagccgatg atcgtcggcg accgggagct
     1141 caacatcacc tgcagcgccg gcatcagcct ctatccgcag gacggtcccg acgtccagac
     1201 gctgctgaag aacgcggacg cggcgatgta ccgcgccaag tccgtggggc gcaacacgtt
     1261 ccagttcttc acggccgaga tgaacgagct ggccaacgag cgcctctcga tggagcaggc
     1321 gctgcgccgc gcgatcgagc gcgacgagct gctgctgcac taccagccgc gcctgaacct
     1381 gctcacgggc gaggtcgacg gtgtcgaggc gctggtgcgc tggatgcatc cggtgcgcgg
     1441 cctcacctat cccgaccgct tcatctcgct cgcggaggag acgggcctca tcgtcccgat
     1501 cggcgaatgg gtcctgcgca cggcgtgcga gcagggacgc gactggcggc gcgccggatt
     1561 ccggccggtg atctcggtga acctgtcggc gcgccagctc tggggcggag gcctcgtgcg
     1621 gctcgtcggc gacgtgatcg ccgagaccgg catgagcgag cagctcgagc tggaactcac
     1681 cgagagcatg gtgatgcacg atgccgagaa cgtgatcgcg acgatgctgg ggctgaaggc
     1741 gctcggagtg cgcctttcgg tggacgactt ctg
//
LOCUS       DASVII010000091         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_580002,
            whole genome shotgun sequence.
ACCESSION   DASVII010000091 DASVII010000000
VERSION     DASVII010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580002"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1076)
                     /gene="cysD"
                     /locus_tag="VF309_01205"
     CDS             complement(96..1076)
                     /gene="cysD"
                     /locus_tag="VF309_01205"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX7465228.1"
                     /translation="MSEKLVSGTHFGEDLDHHSKCVPDTNFEARLSHLDWLESEAIHI
                     MREVVAETRNPALLFSGGKDSIVMLRVAQKAFRPDRFPFPLLHIDTEHNFPEVTAFRD
                     ARAAELGERLIVRSVGESIRAGRVKLRRPDESRNVHQTVTLLDAVKEFEFDALFGGAR
                     RDEEKARAKERILSHRDEFGQWDPRNQRPELWSLYNARLAPGEHLRVFPLSNWTELDI
                     WQYIARENLAVPQIYFAHEREVVVRNGLLAPVTGLTPPRAGETVVTRRVRFRTVGDIT
                     CTCPVLSNAVDVAGIVAETAAAFTSERGATRMDDQTSDASMEQRKKEGYF"
     gene            complement(1094..>1501)
                     /locus_tag="VF309_01210"
     CDS             complement(1094..>1501)
                     /locus_tag="VF309_01210"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX7465229.1"
                     /translation="EGVDERKACCNVRKVEPLRRALAGRRGWITGLRREQADSRAALN
                     EVEHDAATGKWKFSPLADWTNDDVWQYLRANRVPYNSLHDRGYPSIGCEPCTRAVKPG
                     EHPRAGRWWWENAGARKECGLHEIPTRVVETVA"
BASE COUNT          220 a          504 c          526 g          251 t
ORIGIN      
        1 cgtgctcttg ccgtcgtcca cgctgccgca ggtcatgaag cgcaggagcc cgcgatcctc
       61 cagctggagg tcgggcgcgg ccgggagggc gtacatcaga agtacccctc cttcttgcgc
      121 tgctccatgg aggcgtccga agtctggtcg tccatgcgcg tcgccccgcg ctcggaagta
      181 aatgccgcgg cggtctcggc gacaatgccc gcgacgtcga cggcattgga aagcacgggg
      241 cacgtgcagg tgatgtcgcc cacggtacgg aagcgcacgc ggcgcgtgac cacggtctca
      301 cccgcgcgcg gcggcgtgag gccggtcacc ggcgcgagga ggccgttgcg cacgaccacc
      361 tcgcgctcgt gcgcgaaata gatttgcggg accgccaggt tttcgcgcgc gatgtattgc
      421 cagatgtcga gctcggtcca gttggagagc gggaagacgc gcaggtgctc gccgggggcg
      481 aggcgcgcgt tgtagaggct ccagagctcc gggcgctggt tgcgcgggtc ccattggccg
      541 aactcgtcgc ggtgcgagag gatgcgctcc ttcgcgcgcg ctttctcctc gtcgcggcgc
      601 gcgccgccga agagggcgtc gaactcgaat tccttcacgg cgtcgaggag ggtcacggtc
      661 tggtgcacgt tgcggctctc gtcggggcgg cgcagcttca cgcgtcccgc gcggatcgac
      721 tcgcccacgg agcgcacgat gagccgctcg ccgagctccg ccgcgcgcgc gtcgcgaaac
      781 gccgtcacct cgggaaagtt gtgctcggtg tcgatgtgca gcagcgggaa cggaaaccgg
      841 tcggggcgga acgccttctg cgcgacgcgc agcatgacga tcgagtcctt gccgcccgag
      901 aagagcagcg ccggattgcg cgtctccgcg accacctcgc gcatgatgtg gatcgcctcg
      961 ctctccagcc aatcgaggtg cgaaagacgg gcctcgaaat tggtgtcagg tacgcatttc
     1021 gaatggtgat ccaggtcttc gccgaaatgc gtacctgaca ccaatttctc tgacatcagt
     1081 tttgcaacag cgctcatgca accgtctcca ccacgcgggt cgggatctcg tgcaggccgc
     1141 actccttgcg ggcgccggcg ttctcccacc accagcggcc cgcgcggggg tgctcgccgg
     1201 gcttcacggc gcgcgtgcag ggctcgcagc cgatcgacgg gtagccgcga tcgtgcagcg
     1261 agttgtaggg gacgcgattg gcgcgcaggt actgccacac gtcgtcgttc gtccagtcgg
     1321 cgagcgggct gaacttccac ttgccggtgg cggcatcgtg ctcgacctcg ttcagcgcgg
     1381 cgcggctgtc ggcctgctcg cggcgcagcc ccgtgatcca gccgcggcgc ccggcgagcg
     1441 cgcggcgcaa aggctcgacc ttgcgcacgt tgcagcacgc cttgcgctcg tctacgccct
     1501 c
//
LOCUS       DASVII010000092         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_687855,
            whole genome shotgun sequence.
ACCESSION   DASVII010000092 DASVII010000000
VERSION     DASVII010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687855"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF309_01215"
     CDS             complement(<1..192)
                     /locus_tag="VF309_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX7465230.1"
                     /translation="MRYLRWILRSVLFLLLLGFAIKNTDPVVVRYFLGWEWRAPLTLV
                     LLIFFAGGAGLGALAGVGWL"
     gene            complement(231..530)
                     /locus_tag="VF309_01220"
     CDS             complement(231..530)
                     /locus_tag="VF309_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX7465231.1"
                     /translation="MTKSQLIELLSARHSQLAPKDAELAVKTMLDAMALTLASGNRIE
                     IRGFGSFGLNYRPPRTGRNPKTGEKVQVPRKYVPHFKAGKELRERVDAQRDEQQQ"
     gene            complement(562..>1731)
                     /locus_tag="VF309_01225"
     CDS             complement(562..>1731)
                     /locus_tag="VF309_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7465232.1"
                     /translation="QLGEDPWVGISRRYPQGTRLFGKVTNLTDYGAFVEVEAGIEGLV
                     HVSEMDWTNKNVHPSKVVQVGDEVEVMILEIDEERRRISLGMKQLGEDPWVGLSRRYP
                     TGTRLFGKVTNLTDYGAFVEIESGIEGLVHVSEMDWTNKNVHPAKVVSLGDEVEVMIL
                     EIDEDRRRISLGMKQCMPNPWEEFAMNHKKGDKVRGTIKSITDFGVFIGLPGNIDGLV
                     HLSDLSWNAPGEEAVKSLKKGDEVETVVLSIDVERERISLGIKQLDGDPFGNYVTTHD
                     KGNVVKGIVKQIDPKGALIELEKDVDGYLRASEIARDRVEDVRTHLKEGDEVEAVIIN
                     IDRKSRSINLSIKQKDAAEEQAALQKHTSESSASAGTTNLGALLKAKLSGSKDKE"
BASE COUNT          283 a          582 c          506 g          360 t
ORIGIN      
        1 caaccacccg acgcccgcca aggcgccaag gccggcacca ccggcgaaga agatcagcag
       61 gaccagtgtc agaggcgcgc gccattccca acccaggaaa tagcgcacga caaccggatc
      121 ggtgttcttg atagcgaagc ccagaaggag cagaaagagg acgctccgca agatccagcg
      181 cagataccgc atcaactccg tccaaagcct cgcccgccgg ggccgcgagg ttactgctgc
      241 tgttcgtccc gctgcgcgtc cacacgctcg cgcagttcct tgcccgcctt gaaatgcggg
      301 acgtatttgc gaggcacctg caccttctcg ccggtcttcg gattgcgtcc ggttcgcggc
      361 gggcggtagt tcaagccgaa gcttccgaac ccgcggatct cgatgcggtt gcccgacgcc
      421 agcgtgagcg ccatcgcatc cagcatcgtc ttcaccgcga gctcggcatc cttcggtgcc
      481 agctggctat gcctcgcgct caacagctcg atgagctgtg actttgtcat ggcgcgtttc
      541 tcccccctgg agatatttcg attattcctt gtccttcgag cccgacagct tcgccttcag
      601 caacgcgccc aggttcgtgg tgccggcgct cgcgctgctc tcggacgtgt gcttctgcag
      661 cgcggcctgc tcctccgccg cgtctttctg cttgatggac aggttgatcg agcggctctt
      721 gcggtcgatg ttgatgatca ccgcctcgac ctcgtcgcct tccttcaggt gcgtacgcac
      781 gtcctcgacg cggtcgcgcg cgatctcgga agcacgcaga tacccgtcga cgtccttctc
      841 gagctcgatc aacgcgccct tgggatcgat ctgcttcacg atgcccttca cgacgttgcc
      901 cttgtcgtgg gtggtcacgt agttgccgaa cggatcgcca tcgagctgct tgatgccgag
      961 cgagatgcgc tcgcgctcga cgtcgatgga gagcaccacg gtctcgacct cgtcgccctt
     1021 cttcagggac ttaaccgctt cctcgcccgg agcgttccag gagaggtccg aaaggtgcac
     1081 caggccgtcg atgttgcccg gcaggccgat gaacacgccg aagtcggtga tcgacttgat
     1141 cgtgccgcgg accttgtcgc ccttcttgtg gttcatcgcg aactcttccc acgggttcgg
     1201 catgcactgc ttcatgccga gcgagatgcg gcgccggtcc tcgtcgatct cgaggatcat
     1261 cacctcgacc tcgtcgccga gcgacaccac cttcgcggga tgcacgttct tgttggtcca
     1321 gtccatctcg gagacgtgca ccaggccttc gatgcccgat tcgatctcca cgaacgcgcc
     1381 gtagtcggtg aggttggtga ccttgccgaa cagacgggta ccggtggggt agcggcgcga
     1441 caggcccacc catgggtctt cgccgagttg cttcatgccg agcgagatcc ggcggcgctc
     1501 ctcgtcgatc tcgaggatca tgacttccac ctcgtcgccg acctgcacca ccttggaggg
     1561 gtgcacgttc ttgttggtcc agtccatctc ggacacgtgc accaggccct cgatgcccgc
     1621 ctcgacttcg acgaaggcgc cgtagtcggt gagattggtg actttgccga aaaggcgcgt
     1681 tccttgagga tatctcctcg atattccgac ccaagggtct tcgcccagct g
//
LOCUS       DASVII010000093         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_40772, whole
            genome shotgun sequence.
ACCESSION   DASVII010000093 DASVII010000000
VERSION     DASVII010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40772"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF309_01230"
     CDS             <1..253
                     /locus_tag="VF309_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7465233.1"
                     /translation="RPGALAAALDACNRYRTSTQALNSLQVPTLVVAGRRDQMTSFKA
                     GKAVAGEIPGARFVALDAGHSMMSEAPRELLAALREFLG"
     gene            complement(245..>1796)
                     /locus_tag="VF309_01235"
     CDS             complement(245..>1796)
                     /locus_tag="VF309_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX7465234.1"
                     /translation="LSSTLFNELRDVVFVKVAQRAMRRIALEVFHHLHALSLRFHLER
                     QTGGLTRDIERGTRGISTLLSFMVFSVLPTLLEIALVTGFLIYKFDIWFGVITVGALI
                     LYVALTFTISEWRTAHRRQMNEMDSKANTKAIDSLLNYETVKYFGNEQYEAKRYDGNL
                     QSYERAVVKSETSLGLLNAVQALVIALAVSLLMWRAADGIVNGHLTLGDLVMVNALLI
                     QLYIPLNFLGVMYREIKQAVVDMEKMFRLLGENREVEDKPGAPPLAVTGGAIRFENVD
                     FTYDGKREILKNVSFEVPPGRKVAVVGTSGSGKSTLARLLFRFYDVNHGRITIDGQDI
                     RDVQQASVRAALGVVPQDTVLFNDSIYYNVSYGRPGATRDEIENAARVAHIAEFIARL
                     PDGWETMVGERGLKLSGGEKQRVSIARTVLKNPPILVFDEATSALDSHTEKSIQAELA
                     EISRDRTTLVIAHRLSTIVDADEILVLEHGEIVERGNFRDLLAMNGRFAQMWRLQQEE
                     RLEEEALA"
BASE COUNT          296 a          589 c          594 g          317 t
ORIGIN      
        1 gaggcccggg gccctcgccg cggccctcga tgcctgcaat cgctaccgca cctcgacgca
       61 agcgctcaat tcgctgcaag tgcccacgct ggtggtcgcg ggacggcgcg accagatgac
      121 ttccttcaag gcagggaagg cggtcgcggg agagattccc ggcgcgcgat tcgtcgcgct
      181 cgacgcgggg cattcgatga tgagcgaggc gccccgggag ctgctggcgg cgctgaggga
      241 atttctaggc tagggcctct tcctcgagcc tttcttcctg ctgcaggcgc cacatctgcg
      301 cgaagcgccc gttcatggcg agaaggtcgc ggaaattgcc gcgctccacg atctcgccgt
      361 gctcgagcac caggatctcg tcggcatcga cgatggtcga aaggcgatgc gcgatgacca
      421 gcgtcgtgcg gtcgcgcgag atctcggcga gttctgcttg aattgacttc tcggtgtgcg
      481 agtcgagggc cgacgtcgcc tcgtcgaaca ccaggatcgg cggattcttc aggacggtgc
      541 gcgcgatcga cacgcgctgc ttctcgccgc cggagagctt caacccgcgc tcgccgacca
      601 tcgtctccca tccatccggc aggcgcgcga tgaactcggc gatgtgcgcc acgcgcgccg
      661 cattctcgat ctcgtcgcgg gtcgccccgg ggcgcccgta cgacacgttg tagtagatcg
      721 agtcgttgaa gagcaccgtg tcctgcggca ccacgcccag cgccgcgcgc acgctcgcct
      781 gctgcacgtc gcggatgtcc tggccgtcga tggtgatgcg cccgtggttc acgtcgtaga
      841 agcggaagag aaggcgcgcg agcgttgact tccccgagcc gctcgtcccg accaccgcga
      901 ctttcctgcc cggcggcacc tcgaagctca cgttcttcag gatctcgcgc ttgccgtcgt
      961 aggtgaagtc gacattctcg aagcggatcg ccccgcccgt caccgcgagc ggcggcgcgc
     1021 cgggcttgtc ctcgacctcg cggttctcgc cgaggagccg gaacatcttc tccatgtcga
     1081 ccaccgcctg cttgatctcg cgatacatca cgccgaggaa gttcagcggg atgtagagct
     1141 ggatgaggag cgcgttcacc atcaccaggt cgcccaacgt gaggtggccg ttcacgatgc
     1201 cgtcggccgc gcgccacatg aggagcgaca ccgcgagcgc gatcaccagc gcctgcaccg
     1261 cgttcagcaa tcccagggag gtctcgctct tcaccaccgc gcgctcgtag ctctgcagat
     1321 tcccgtcgta gcgcttcgcc tcgtactgct cgttgccgaa gtacttgacc gtctcgtagt
     1381 tgaggaggga gtcgatcgcc ttggtgttgg ccttcgagtc catctcgttc atctggcggc
     1441 gatgcgccgt gcgccattcc gagatcgtga aggtgagcgc gacgtagagg atcagggcgc
     1501 ccaccgtgat cacgccgaac cagatgtcga acttgtagat gaggaagccc gtgacgagcg
     1561 cgatctcgag cagcgtcggc agcacgctga agaccatgaa cgagaggagc gtcgagatgc
     1621 cgcgggtgcc gcgctcgatg tcgcgggtga gtccgcccgt ctggcgctcg aggtggaagc
     1681 gcagcgacaa cgcgtggagg tggtggaaga cctcgagcgc gatgcgccgc atggcgcgct
     1741 gcgcgacctt cacgaagacg acgtcccgca gctcgttgaa gagcgtgctg gaaagc
//
LOCUS       DASVII010000094         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_148408,
            whole genome shotgun sequence.
ACCESSION   DASVII010000094 DASVII010000000
VERSION     DASVII010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148408"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1140)
                     /locus_tag="VF309_01240"
     CDS             complement(10..1140)
                     /locus_tag="VF309_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX7465235.1"
                     /translation="MDFLPFARPTIDEAMIAAVADTLRTRWIVTGPRVAAFEKELSKR
                     FSDRPVRALTSATAAMQVALELMGIGPGDEAIIPAQSFFVTGNVVERAGATAVFVDVN
                     LASRNIDFAQAAAAVTAKTRLLFPTHYNAPLDPDVLDAFAKRHKVRILEDAALAIGSR
                     TSRGPVGASGDLVSFSFHPNKNMTTIEGGALVLNDEREAAIVDELRFHGIKRLPDGTR
                     DVERAGAKYNFSDVSARLGLEQLTHLDTWCRARERLVHRYFECLAGDDLLVPERLPPR
                     DNPGHSWNMFTVLLPLADLGMSRKAFMDAMMQEGIGTGVSYEAIHLTTLFRKKGFREG
                     QFPVSERIGRETVTLPLFPEMRETDVDRVCETMKRVLRRKAS"
     gene            complement(1173..>2142)
                     /locus_tag="VF309_01245"
     CDS             complement(1173..>2142)
                     /locus_tag="VF309_01245"
                     /inference="COORDINATES: protein motif:HMM:NF037277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX7465236.1"
                     /translation="WFLAVSSANPEFARFFFIHEHFERFLTQEHRRVQPAWFFVPILF
                     AGFLPWMFALPAAIAIAWRGEARRDFQPLRLAILWGAFVLAFFSASGSKLPAYILPLF
                     PPLALVVGRYLVTAPARRLALWVAPGIPFALALGWLAWRYPSDAKDEWTAAMYLGAQP
                     WALAAAMVHLAASVAAVTLLRKERRGAAVATIAFAAILLVGCLEMGYRHLSPRQSGFE
                     VAERMKPYLTPATRVYSVSHYDQTIPFYIARTVTLVAYVDEFETGEKAEPALWIPGIE
                     AFAAQWQRPGDALAIMQPGAFEKSKAAGLPMQVLHEDPRRVLVRKP"
BASE COUNT          354 a          699 c          783 g          306 t
ORIGIN      
        1 gccatcgggc taggacgcct tgcggcgcag cacgcgcttc atcgtctcgc acacgcggtc
       61 cacgtcggtc tcgcgcatct cgggaaagag cggaagcgtg accgtctcgc gcccgatgcg
      121 ctcggagacc ggaaactggc cttcgcggaa gccctttttg cggaagagcg tcgtgagatg
      181 gatcgcttcg taggaaacgc cggtgccgat gccctcctgc atcatcgcgt ccatgaaggc
      241 cttgcgcgac atgcccaggt cggcgagcgg caggaggacg gtgaacatgt tccagctgtg
      301 cccgggattg tcgcgcggcg gcagccgctc gggcacgagg aggtcgtcgc ccgcgaggca
      361 ttcgaagtag cggtgcacga ggcgctcgcg cgcgcgacac cacgtgtcga gatgcgtgag
      421 ctgctcgagg ccgaggcgcg ccgacacgtc ggaaaagttg tacttggcgc cggcgcgctc
      481 gacgtcgcgg gtgccgtcgg gcaggcgctt gatgccgtgg aagcgaagct cgtccacgat
      541 cgcggcctcg cgctcgtcgt tcagcaccag cgcgccgccc tcgatggtgg tcatgttctt
      601 gttgggatgg aagctgaagg agaccaggtc gccgctcgcg ccgacggggc cgcgcgaggt
      661 gcgcgagccg atggcgagcg ccgcgtcctc gaggatgcgc accttgtgcc gcttggcgaa
      721 cgcgtcgagc acgtccggat cgaggggcgc gttgtagtga gtggggaaga gaagccgcgt
      781 cttcgcggtc accgccgccg ccgcctgggc gaagtcgatg ttgcgcgacg cgagattcac
      841 gtcgacgaag accgcggtcg cgccggcgcg ctcgaccacg ttgccggtca cgaagaagct
      901 ctgcgccgga atgatggcct cgtcgccggg gccgatcccc atcagctcga gcgcgacctg
      961 catcgccgcg gtcgccgagg tgagggcgcg cacgggacgg tcggagaatc gcttcgagag
     1021 ctccttctcg aaggccgcga cgcgggggcc cgtcacgatc catcgcgtgc gcagcgtgtc
     1081 ggctacggcc gcgatcatcg cctcgtcgat ggtcgggcgc gcgaagggca ggaaatccat
     1141 ggttctcggc ggcgcagcgg cagggtgggg gatcagggct tgcggacgag gacgcggcgg
     1201 ggatcttcgt gcagaacctg catgggcaag cccgccgcct ttgatttttc gaacgctccg
     1261 ggctgcatta tagcgagcgc gtcgcccggg cgctgccatt gcgcggcgaa cgcctcgatc
     1321 ccgggaatcc acagcgccgg ctcggccttc tcgccggtct cgaattcgtc cacataggcg
     1381 accagcgtca ccgtgcgcgc gatgtagaac gggatcgtct ggtcgtagtg gctcacgctg
     1441 tagacgcgcg tggcgggcgt gaggtagggc ttcatccttt ccgcgacctc gaatcccgat
     1501 tgccgcggcg aaaggtgccg gtagcccatc tcgaggcatc ccacgaggag gatcgcggcg
     1561 aacgcgatcg tcgcgaccgc cgccccgcgc cgctccttgc gcagcagcgt gaccgccgcg
     1621 acgctcgcgg cgagatgaac catcgccgcc gccagcgccc agggctgcgc gccgagatac
     1681 atcgccgcgg tccactcgtc cttcgcgtcc gacgggtagc gccatgcgag ccagccgagc
     1741 gccagcgcga aagggatccc gggggcgacc cagagggcga gccggcgcgc cggggcggtg
     1801 acgagatagc ggcccacgac gagcgcgagc ggcgggaaca gcggcaggat gtaggcgggc
     1861 agcttcgatc ccgacgcgct gaagaacgcg aggacgaaag cgccccacag gatcgcgagc
     1921 cgcagcggct ggaagtcgcg tcgcgcctcg ccccgccacg cgatcgcgat cgcagcgggc
     1981 agcgcgaaca tccacggcag gaatcccgcg aagaggatcg gcacgaagaa ccacgcgggc
     2041 tgcacgcggc gatgctcctg cgtgaggaag cgctcgaagt gctcgtggat gaagaagaag
     2101 cgggcgaact cgggattcgc gctcgacacc gcgaggaacc ac
//
LOCUS       DASVII010000095         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_651953,
            whole genome shotgun sequence.
ACCESSION   DASVII010000095 DASVII010000000
VERSION     DASVII010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651953"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VF309_01250"
     CDS             <1..617
                     /locus_tag="VF309_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465237.1"
                     /translation="SFEIATGEMVAVTGRSGAGKSTLLKLAAGIERASSGTVVVQGQN
                     LGTLGETALAVLRRRIGFVFQDHKLLFDRNAFENVALPLRVAGFARDEIGRRVRAALD
                     KVGLLAREKAAPVALSGGEQQRLCIARAIVNRPAILIADEPTGNLDAEYAGAILDLFA
                     SFHQVGVTVLLSSHDEASLRRLACRRLSLSGGTVVPMGEELAAS"
     gene            614..1525
                     /locus_tag="VF309_01255"
     CDS             614..1525
                     /locus_tag="VF309_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX7465238.1"
                     /translation="MSIWLRVHWHAFTDAWRRVGSQPLGAAFSVLVLAVAIAIPVMAA
                     VALQSVGAVTSGLETDPHVNVYLALDASDEDARRVGDALSAHPLAAAVKFVPRSQALE
                     ELKASTHLAELLASLDRNPLPHAFTVRVRSTDAATIAQAREAWSRLPKVDQVVADFEW
                     AERLGRWVRFANRILAFVAIGLASAVTFIVGHLIRLQVVSQREEIEVSQLIGATASDV
                     RRPFLYRGLVQGALAGAVAVLLSWAVAWWVGTELRALTPNYAAELKVVFLPLMDCLAI
                     AAGAAGLGLLGAWWAVGRELRQFSPSR"
     gene            1597..2451
                     /gene="rpoH"
                     /locus_tag="VF309_01260"
     CDS             1597..2451
                     /gene="rpoH"
                     /locus_tag="VF309_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX7465239.1"
                     /translation="MASLTLPALPPVSSPESYVQAVNAIPMLTAERELELGRRLRDSD
                     DVTAASGLVLPHLRLVVSVARNYMGYGLPQADLVQEGNIGLMKAVKRFDPERGVRLAS
                     FAIHWIKAEIHEYILRNWRMVKIATTKAQRKLFFNLRSMKKSSKALTPSEIRQVAETL
                     NVKESDVVEMEQRLQGGDIALEPSPEDGEEAPVTPIAYLTETSEGPAELFEREQTEAM
                     NSRGLSAALQSLDERSRRIVRARWLAESDPKTLHELAVEFNVSAERIRQIEAKAMQKM
                     KKQMATLH"
BASE COUNT          406 a          841 c          853 g          426 t
ORIGIN      
        1 tcagcttcga gatcgcgacg ggcgagatgg tcgccgtcac cggccgctcc ggcgcaggaa
       61 aatcgacgct cctcaagctc gctgcgggca tcgagcgcgc ctcgagcgga acggtggtgg
      121 tgcaggggca gaacctcggc accttgggcg aaaccgcgct cgcggtgctt cgccgccgca
      181 tcggcttcgt cttccaggac cacaagcttc tcttcgatcg caacgcgttc gagaacgtcg
      241 cgcttcccct gcgcgtcgcg gggttcgcgc gcgacgagat cggccggcgc gtgcgtgccg
      301 cgctcgacaa ggtggggctc ctcgcgcgcg agaaggcggc ccccgtcgcg ctctccggag
      361 gcgagcagca gcgcctctgc atcgcgcgcg cgatcgtcaa ccggcccgcg atactgatcg
      421 ccgacgaacc cacgggaaac ctcgacgccg aatacgcggg agcgatcctc gatctcttcg
      481 cctcgttcca ccaggtggga gtcacggtgc tgctgtcgag ccacgacgag gcgagcctgc
      541 ggcgcctcgc ctgccgtcgc ctgtcgctct cgggcgggac ggtcgtgccc atgggcgaag
      601 agctcgcggc ctcatgagca tctggcttcg cgtgcactgg cacgcattca cggacgcgtg
      661 gcggcgcgta ggctcccagc cgctcggcgc cgcgttctcc gtgctggtgc tggcggtcgc
      721 gatcgccatt ccggtgatgg ccgcggtcgc gctgcagagc gtgggcgccg tgacctcggg
      781 gctggagacc gatccgcacg tgaacgtcta cctcgccctg gatgcgagcg acgaggatgc
      841 gcgtcgcgtc ggcgatgctt tgagcgccca tcccctggcg gcggccgtga aattcgtgcc
      901 gcgctcgcaa gcgctcgagg agctgaaggc gtctactcac ctcgccgagc ttctcgcgag
      961 cctcgatcgc aatcccctgc cccatgcgtt caccgtacgc gtgcgaagca ccgatgcggc
     1021 gacgatcgcg caggcacgcg aggcttggtc gaggcttccg aaggtcgacc aggtcgtggc
     1081 ggatttcgaa tgggcggagc gcctcggccg ctgggtccgc ttcgccaacc gcatcctggc
     1141 attcgtcgcc atcgggctcg cgagcgcggt caccttcatc gtcggtcacc tgatccgcct
     1201 tcaggtcgtc tcgcagcgcg aggagatcga ggtgagccag ctcatcggcg ccaccgcatc
     1261 ggacgtgcgc aggccgttcc tgtaccgcgg cctggtgcag ggtgccctgg cgggcgcggt
     1321 ggcggttctt ctcagctggg ccgtcgcctg gtgggttggc acggaattgc gagcgcttac
     1381 tcccaactac gccgctgaac ttaaggttgt tttcctgcct ctaatggact gcttggccat
     1441 tgcggccgga gcggccgggc tggggcttct cggggcctgg tgggcggttg gccgggagct
     1501 tcggcagttc tcgccctcga ggtaggaact catccgcccc ttagcactct aatcagctga
     1561 gtgctaaaat cgagccaaca ggaggtagac caccccatgg caagcttgac cctgccggcc
     1621 cttccgccgg tatccagccc ggaatcctat gtccaggccg tgaacgcgat tccgatgctg
     1681 actgcggagc gcgagctcga gctcgggcgc cgcttgcgcg atagcgacga cgtaacggcc
     1741 gcgagcggct tggtgctgcc gcacttgcgc ctcgtcgtgt cggtcgcgcg gaactacatg
     1801 ggctatggcc tgccccaggc ggacctggtg caggaaggca acatcggcct catgaaggcc
     1861 gtcaagcgtt tcgacccgga gcgcggcgtg cgcctcgcgt cgttcgcgat ccattggatc
     1921 aaggccgaga ttcacgaata catcctgcgc aactggcgga tggtgaagat cgccaccact
     1981 aaggcccaac gcaagctgtt cttcaacttg cgcagcatga agaagagctc caaggcgctc
     2041 acccccagcg agatccggca ggtcgccgag accctgaacg tgaaggaaag cgacgtcgtg
     2101 gagatggagc agcgcctcca gggtggagac atcgccctcg agccctcgcc ggaggatggc
     2161 gaggaagcgc ccgtcacgcc gattgcatac ctcacggaaa cctccgaggg acccgccgag
     2221 ctgttcgagc gcgagcagac cgaggcgatg aacagccgcg ggctttccgc ggcgctgcag
     2281 agcctcgatg agcgcagccg ccgaatcgtg cgggcacgct ggctcgcgga gtcggatccg
     2341 aagacgcttc acgagctcgc cgtcgaattc aacgtatcgg ccgagcgcat ccgccagatc
     2401 gaagcgaagg cgatgcagaa gatgaagaag cagatggcga cgctccactg aggtcgccgg
     2461 cgagccattc gcaaacgggg cttcggcccc gttcttgttt ggggatggct ccgcctcgaa
     2521 gcgttc
//
LOCUS       DASVII010000096         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_40862, whole
            genome shotgun sequence.
ACCESSION   DASVII010000096 DASVII010000000
VERSION     DASVII010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40862"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="VF309_01265"
     CDS             <1..1775
                     /locus_tag="VF309_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7465240.1"
                     /translation="EVNEALAEETSEKLRAQQRELLAAQKLRMHFERTPLAVLEWDRE
                     CRIIAWNPAAEAIFGFPAYDAVGKQVPALLGSDTERHNLETMCRELLEAGDGNKTTLG
                     NVTRGGRSIQCEWYNTPLVDAQGKVTGFASLVHDVTERLNTERTIHYMAHHDALTGLP
                     NRRLMQDRLNQAIMSARRKQRHVAVLFLDLDRFKVVNDTPGHDTGDFILKDVARRLSS
                     CVREGDTVSREGGDEFVMILPDLERPEHARVVADKILKELIRPVEIGGHEIHVTPSIG
                     ISHYPNDATDVHQLLKHADNAMYQAKDAGRNTIRFFTNDLNFLLSKRLEIESRLRRAI
                     DNEEFFLRYQPQVELATGRISGMEALIRWNDPQKGEIFPKDFIFVAEELGLIVPIGEW
                     VFRTACRQLRQWEQDGLPPVTISINISPRQFMSRRLVSTLLAIVREIGANPRYIELEI
                     TETMIMRNVEQSIETLSQLRSVGMQVAVDDFGVGYSSLGQLKRLPASSMKIDRSFIMN
                     VPEDSSSGSITEAIIAMAKRLKLRCIAEGVETRAQLEFLRANHCEAFQGYLFSRPVTA
                     LEATAMLKAQAAPHAAETAQSAAD"
BASE COUNT          354 a          688 c          630 g          292 t
ORIGIN      
        1 aggaggtgaa cgaggccctc gccgaggaga cctcggagaa gctgcgcgcg cagcagcgcg
       61 agctcctcgc cgcgcagaag ctgcgcatgc acttcgagcg cacgcccctc gcggtcctcg
      121 aatgggaccg cgagtgcagg atcatcgcgt ggaacccggc ggccgaggcg atcttcggct
      181 tcccggccta cgacgcggtc ggcaagcagg tgcccgccct cctcggctcc gacaccgagc
      241 ggcacaacct cgagaccatg tgccgcgagc tcctcgaggc gggcgacggc aacaagacca
      301 cgctcggcaa cgtgacgcgc ggcggcagga gcatccagtg cgagtggtac aacacgccgc
      361 tggtcgacgc gcagggcaag gtcaccggct tcgcttcgct cgtgcacgac gtcaccgagc
      421 gcctgaatac cgagcgcacc atccactaca tggcgcacca cgacgcgctc accggattgc
      481 cgaaccggcg cctcatgcag gaccgcttga accaggcgat catgtcggcg cgccgcaagc
      541 agcgccacgt cgcggtcctc ttcctcgacc tcgaccgctt caaggtggtg aacgacacgc
      601 ccggccacga caccggcgac ttcatcctga aggacgtggc gcggcgcctg tcgtcgtgcg
      661 tgcgggaggg cgacaccgtg tcgcgcgagg gcggcgacga gttcgtgatg atcctgccgg
      721 acctcgagcg tccggagcac gcgcgggtcg tggcagacaa gatcctgaag gagctgatcc
      781 gcccggtgga gatcggcggc cacgagatcc acgtgacgcc ctcgatcggc atcagccact
      841 accccaacga cgcgaccgac gtgcaccagc tcctcaagca cgccgacaac gcgatgtacc
      901 aggcgaagga cgcgggccgc aacaccatcc gcttcttcac caacgacctg aatttcctcc
      961 tctccaagcg cctcgagatc gagagccgcc tgcgccgggc gatcgacaac gaggagttct
     1021 tccttcgcta ccagccgcag gtggagctcg cgaccgggcg catctcggga atggaagcgc
     1081 tgatccgctg gaacgatccg cagaagggcg agatcttccc caaggacttc atcttcgtcg
     1141 ccgaggagct gggcctcatc gtgccgatcg gcgaatgggt gttccgcacc gcgtgccggc
     1201 agttgaggca gtgggagcag gacgggctgc cgcccgtcac catctcgatc aacatctcgc
     1261 cgcgccagtt catgtcgagg cgcctcgtct cgacgctgct cgcgatcgtc cgggagatcg
     1321 gcgcgaatcc gcgctacatc gagctggaaa tcaccgagac gatgatcatg cgcaacgtcg
     1381 agcagtcgat cgagacgctc tcgcagctgc gctccgtcgg catgcaggtc gccgtcgacg
     1441 atttcggcgt gggctactcg tcgctcggcc agctgaagcg gctgccggcg tcgagcatga
     1501 agatcgaccg ctcgttcatc atgaacgtgc ccgaggacag ctcgagcggc tccatcacgg
     1561 aggcgatcat cgccatggcc aagcgcctca agctgcgctg catcgccgag ggcgtcgaga
     1621 cccgcgccca gctcgaattc ctccgcgcga atcactgcga ggctttccag ggctatctct
     1681 tctcgcgccc cgtgaccgcg ctcgaggcga cggcgatgct gaaggcgcag gccgctcccc
     1741 acgccgccga gacggcgcaa tccgccgccg actagaagcg cggcccgggc gatcgcctcc
     1801 ttgaagtggg ggtggagctc gacccggcgc gatcgttcct tgggagggga gggagctcgc
     1861 tgcaacccat cccgctcgaa ctggcggcgt cttctcccga ttgacgggca gtgacgcgcg
     1921 cctctcgagc tccaccccaa cacttcaatg aggctctcaa gggt
//
LOCUS       DASVII010000097         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_40934, whole
            genome shotgun sequence.
ACCESSION   DASVII010000097 DASVII010000000
VERSION     DASVII010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40934"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF309_01270"
     CDS             complement(<1..424)
                     /locus_tag="VF309_01270"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7465241.1"
                     /translation="MIKQRTLKSIVRASGVGVHTGKKVALALRPAPADTGIVFCRSDL
                     PGNPAIPARALNVTNTTMATVIEKDGARVSTVEHLMSAFAGMGIDNAFVDVSGEEVPI
                     MDGSAGTFVFLIQSAGIEEQAAAKRYLRVLKRVELEDGD"
     gene            complement(588..1736)
                     /gene="ftsZ"
                     /locus_tag="VF309_01275"
     CDS             complement(588..1736)
                     /gene="ftsZ"
                     /locus_tag="VF309_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7465242.1"
                     /translation="MIELLETTTPDAVIKVVGVGGCGGNAVDHMINNGVQGVEFITMN
                     TDAQALKRNLAKTTLQLGTTVTKGLGAGANPEVGRLAAEEDRERIIEMVTGADMLFIT
                     AGMGGGTGTGAAPVVAEIAREMGILTVAVVTKPFAFEGKRQKVAAVGIDQLKQHVDSL
                     IIIPNDRLMQVLGEDVTYEDAFRASNDVLNGAVAGIAEVINCPGMVNVDFADVRTVMS
                     ENGVAMMGSATASGPERAQMAAEQAVHSPLLEDIHLSGARGVLVNITASHTMKLKEVH
                     EVMNTIRSFTAEDATVIYGSVVDEAIGDALRVTIVATGLGGQAAQRTQPMMQMVQRTG
                     TDDASAVNYEELDQPTALRRRRDVTVEAMRHSGVDMLDIPAFLRKQAD"
     gene            complement(1776..3020)
                     /gene="ftsA"
                     /locus_tag="VF309_01280"
     CDS             complement(1776..3020)
                     /gene="ftsA"
                     /locus_tag="VF309_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7465243.1"
                     /translation="MIATRARDTRNLLVALDIGTSKIVTLVAEITAEDTLSLIGMGSH
                     PSRGLKKGVVVNIESTVNAIQRSLEEAELMADAKIKEVLTGIAGSHIKSFNSHGMVAI
                     KDKEVQQYDIDRVIETARAVNIPMEQQVLHILEQEFIIDGQGGVRQPLGMSGMRLEVK
                     VHIVTGAVSAAQNIMKCVRRCGLEVRDLMLQPLASADAVLLDDERDLGVCLVDIGGGT
                     TDIAVFTDGAIKHTSVIPIAGDQITNDIAMALRTPTKDAEELKIAHGVALRQLASASD
                     MIEVPGVGERGPRELSRQTLAEVIEPRVEELYSLIQRELRSSGLEELLSSGIVITGGS
                     ALMKGMVELGEEVFHMPVRVGLPQYSGPLAEVVRNPRYSTAMGLLMAGIDQVKRDRHA
                     KMQGAGFKEVLEKMKGWFKGNF"
     gene            complement(3017..>3249)
                     /locus_tag="VF309_01285"
     CDS             complement(3017..>3249)
                     /locus_tag="VF309_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX7465244.1"
                     /translation="VTLESGLALDLGRGDIHSRLARFVSAWPQLASRGVETRHADLRY
                     ANGFAVRTAEVRALPKPAKSPSRPAARTEKKQ"
BASE COUNT          480 a         1112 c         1047 g          610 t
ORIGIN      
        1 tatcgccgtc ctcgagttca accctcttca acacgcgcag gtagcgcttg gccgcggcct
       61 gctcctcgat tccggcggac tggatcagga agacgaaagt gccggcgctg ccgtccatga
      121 tcggcacttc ctcgccggag acgtcgacga aggcgttgtc gatgcccatg ccggcgaagg
      181 ccgacatcaa atgctcgacg gtggagacgc gcgcgccgtc cttctcgatc accgtcgcca
      241 tggtggtgtt ggtgacgttg agcgccctgg cgggaatcgc cggattgccg ggaaggtcgg
      301 agcgacagaa gacgatgccg gtgtcggccg gtgccggcct cagggcgagg gccaccttct
      361 tgccggtatg aacgcccacg ccgctggcgc gaacgatcga tttgagagtg cgctgcttga
      421 tcattttccc aagtatatca tatgcttaca tcaatgaaaa acgggcccaa cctgcgggcc
      481 cgctgaagtc gatgccgcgt cgccggcggg agactcgccg gcgatgcacg tcgttcccgc
      541 gaaggcggga atccaggctg ggtccccgcc ttcgcgggga cgacgtccta gtccgcctgc
      601 ttgcgcaaga acgccgggat gtcgagcatg tcgactcccg aatgccgcat cgcctcgacc
      661 gtcacgtccc tgcggcgccg cagcgccgtc ggctggtcga gctcctcgta gttcaccgcc
      721 gacgcgtcat ccgtgccggt gcgctgcacc atctgcatca tcggctgggt gcgttgcgcg
      781 gcctggccgc cgagccccgt cgcgacgatc gtcacgcgca gcgcatcgcc gatcgcctcg
      841 tcgacgacgc ttccatagat cacggtcgcg tcctccgcgg tgaacgagcg gatggtgttc
      901 atcacctcgt ggacttcctt cagcttcatc gtgtggctcg cggtgatgtt caccagcaca
      961 cccctcgcgc ccgaaagatg aatatcctcg aggagcggcg aatgcacggc ttgttcggcc
     1021 gccatctgcg ccctctcggg tccggacgcc gtggccgagc ccatcatcgc gaccccgttc
     1081 tcgctcatga cggtgcgcac gtcggcgaaa tcgacgttca ccatgccggg gcagttgatg
     1141 acttccgcga tgcccgcgac ggcgccgttc agcacgtcgt tcgaggcgcg gaatgcgtct
     1201 tcgtaggtca catcctcccc gaggacctgc atgaggcgat cgttggggat gatgatgagc
     1261 gaatcgacgt gctgcttcag ctggtcgatt cccaccgcgg cgaccttctg gcgcttgccc
     1321 tcgaacgcga agggcttggt cacgaccgcg accgtgagga tgcccatctc gcgcgcgatc
     1381 tcggcgacca cgggcgccgc gcccgttccc gtgccgccgc ccatgcccgc ggtgatgaag
     1441 agcatgtccg cgccggtcac catctcgatg atgcgctcgc ggtcctcctc ggcggcgagc
     1501 ctccccacct cgggattcgc gcccgcgccc agccccttcg tgacggtggt gccgagctgc
     1561 agcgtcgtct tggcgaggtt cctcttgagc gcctgcgcgt cggtgttcat ggtgatgaac
     1621 tccacgccct gcactccgtt gttgatcatg tggtcgaccg cgttgccgcc gcagcctccc
     1681 acgcccacca ccttgatcac cgcatccggt gtcgtagttt ccaacagctc gatcatcatc
     1741 gtctcctttg ttgtgtacga cttccctgcc ccacgtcaga aattgccctt gaaccagccc
     1801 ttcatcttct ccagcacttc cttgaatccc gcgccctgca tcttcgcgtg gcgatcgcgc
     1861 ttcacctggt cgatgcccgc catgagcagg cccatcgccg tggagtagcg cggattgcgg
     1921 acgacttcgg cgagcgggcc gctgtactgc ggcaggccca cgcgcaccgg catgtggaag
     1981 acctcctcgc cgagctcgac catgcccttc atcagggccg atcccccggt gatcacgatc
     2041 cccgacgaga gcagctcctc gaggcccgac gatcgcaatt cccgctggat cagcgaatag
     2101 agctcctcga cgcgcggctc gatcacttcc gcgagcgtct ggcgcgagag ctcgcgcgga
     2161 ccgcgctcgc ccacgccggg gacctcgatc atgtcgctcg cgctcgcgag ctggcgcagc
     2221 gccacgccgt gcgcgatctt cagctcctcg gcgtccttgg tcggcgtgcg caacgccatc
     2281 gcgatgtcgt tggtgatctg gtcgcccgcg atcgggatga ccgacgtgtg cttgatcgcg
     2341 ccgtcggtga agaccgcgat atcggtggtg ccgccgccga tgtcgacgag gcatacgccc
     2401 aggtcgcgct cgtcgtcgag gaggaccgcg tcggcggaag cgagcggctg cagcatgagg
     2461 tcgcgcacct ccagcccgca gcggcgcacg cacttcatga tgttctgcgc ggccgacacc
     2521 gcgcccgtga cgatgtgcac cttcacctcg aggcgcatgc cgctcatgcc gaggggctgg
     2581 cgcacgccgc cctgcccgtc gatgatgaat tcctgctcga ggatgtggag gacctgctgc
     2641 tccatcggga tgttcacggc gcgggccgtc tcgatcacgc ggtcgatgtc gtactgctgg
     2701 acctccttgt ccttgatggc gaccatgccg tgcgagttga agctcttgat gtggctgccc
     2761 gcgatcccgg tgagcacttc cttgatcttc gcgtcggcca tgagctcggc ctcctcgagg
     2821 gagcgctgga tcgcgttcac cgtggactcg atgttcacga cgacaccctt cttcagcccg
     2881 cgcgacgggt ggcttcccat gccgatgagg ctcagcgtgt cctcggccgt gatctcggcg
     2941 acgagggtca cgatcttgga ggtgccgatg tcgagggcga cgaggaggtt gcgggtatcg
     3001 cgggcgcggg tggcgatcat tgcttctttt ccgtgcgcgc cgccgggcgc gaaggggatt
     3061 tggcgggctt gggcagcgcc cgcacctcgg ccgtccgaac agcgaagccg ttggcgtagc
     3121 gaaggtcggc gtggcgcgtc tcgacgccgc gcgaggcgag ctgcggccac gcggagacga
     3181 agcgcgcgag gcgcgaatgg atgtcgcctc gccccaggtc gagggcaagg cccgattcga
     3241 gggtcacct
//
LOCUS       DASVII010000098         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_256733,
            whole genome shotgun sequence.
ACCESSION   DASVII010000098 DASVII010000000
VERSION     DASVII010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256733"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="wrbA"
                     /locus_tag="VF309_01290"
     CDS             <1..448
                     /gene="wrbA"
                     /locus_tag="VF309_01290"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX7465245.1"
                     /translation="APPVPGEGAPYASHEDLTQCAGLILGSPTRFGNMAAPLKHFIDG
                     LGPEWARGTLAGKPAAVFSSTATMHGGQEVTLVSMMVPLLHHGMLIVGLPYTEPELNS
                     TRSGGTPYGATHVSGTANDRPVSDEEARLAFALGKRLASVAKKLAP"
     gene            445..786
                     /locus_tag="VF309_01295"
     CDS             445..786
                     /locus_tag="VF309_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEX7465246.1"
                     /translation="MSARVPWLAACASLIALIFLDVAWELFLAPLKPGGSWMVLKALP
                     LLAPLWGVLHGRRYTYQWSTLLIWPYAAEGAARAYTDSGLSAGLALAELALSLAYFFS
                     VASFLKAGRKD"
     gene            complement(738..1226)
                     /locus_tag="VF309_01300"
     CDS             complement(738..1226)
                     /locus_tag="VF309_01300"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX7465247.1"
                     /translation="MAVDSHIFDISRTIQLAVAPVFLLTAIGTIINVLIGRLGRAVYR
                     RRLLEEHLSEYSGEMRDDAVTELSMLAHRVVLVLWSVTLAVLGALLVCLLIGVAFAGA
                     YFSMDLSREVAALFVAAMVAVTFCLLLFLREISIAAVSAHQTLRPQSLRPAFRNDATE
                     KK"
     gene            complement(1232..>1534)
                     /locus_tag="VF309_01305"
     CDS             complement(1232..>1534)
                     /locus_tag="VF309_01305"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7465248.1"
                     /translation="AFYERHGGKAVVISRFLPIVRTYVPFVAGMAQMSAREYLAYNIA
                     GAVLWVGTLTYAGFFFGNIAWIHANLTAIIVAIVVVSLLPLVYAYVKSRRSPRPQP"
BASE COUNT          270 a          513 c          530 g          221 t
ORIGIN      
        1 cgcgccgccg gtgcccgggg aaggcgctcc ttacgcgagc catgaagacc tgacgcagtg
       61 cgccgggctg atattgggaa gtcccacccg cttcggcaac atggcggcgc cgctcaagca
      121 cttcatcgac ggcctcggcc ccgaatgggc gcgcggcacg ctcgcgggaa agcccgcggc
      181 ggtgttctcc tccacggcga cgatgcacgg aggccaggaa gtcaccctgg tatcgatgat
      241 ggtgccgctg ctgcaccatg gaatgctcat cgtcggcctg ccctacaccg agcccgagct
      301 caactcgacg cgctccggcg gcacgcccta cggcgcgacg cacgtctcgg gcacggcgaa
      361 cgacagaccc gtgagcgacg aggaagcgag gctcgcgttc gcgctcggca agcgcctcgc
      421 gagcgtcgcg aagaagctcg cgccttgagc gcgcgggtgc catggctcgc ggcgtgcgcg
      481 agcctgatcg cgctgatatt cctcgacgtc gcgtgggagc tcttcctcgc gcccctcaag
      541 cccggcggct cgtggatggt gctgaaggcg ctgccgctcc tggcgccgct ttggggcgtg
      601 ctccacggaa ggcgctacac ctaccagtgg tcgacgctcc tcatctggcc gtacgccgcc
      661 gagggcgccg cgcgagccta cacggacagc ggattgtcgg cggggctcgc gctggccgag
      721 ctcgccttgt cgctcgccta tttcttctcg gtggcgtcgt tcctgaaggc cggccgcaag
      781 gactgaggcc tgagcgtctg gtgcgcgctc accgcggcga tcgagatctc cctgaggaac
      841 aggagcaggc agaaggtgac cgcgaccatg gcggcgacga agagcgccgc gacctcgcgc
      901 gacagatcca tcgagaagta cgcgcccgcg aaggcgaccc cgatgagcag gcacaccagc
      961 agcgcgccca gcaccgcgag cgtcacggac cacaggacga gcacgacccg gtgcgcgagc
     1021 atgctcaatt cggttaccgc gtcgtcgcgc atctcgcccg aatattccga gagatgctcc
     1081 tcgaggagac ggcggcgata gacggcgcgc ccgaggcggc cgatcagcac gttgatgatg
     1141 gtcccgatcg cggtcagcag gaacacgggc gcgaccgcga gctggatggt gcgcgagatg
     1201 tcgaagatgt gggagtcgac ggccacggga atcatggttg aggccgcggg gaccggcggc
     1261 ttttcacgta ggcgtagacg agcggcagga ggctcacgac cacgatcgcg acgatgatcg
     1321 cggtgaggtt tgcatggatc cacgcaatgt tgccgaagaa gaagcccgca taggtcaacg
     1381 tccctaccca gagcaccgcg cccgcgatgt tgtacgcaag gtactcccgc gcggacatct
     1441 gcgccattcc cgcgacgaag ggcacgtagg tgcgcacgat cggcaggaag cgcgagatca
     1501 ccaccgcctt gccgccgtgg cgctcgtaga aggc
//
LOCUS       DASVII010000099         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_76882, whole
            genome shotgun sequence.
ACCESSION   DASVII010000099 DASVII010000000
VERSION     DASVII010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76882"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1100)
                     /locus_tag="VF309_01310"
     CDS             complement(180..1100)
                     /locus_tag="VF309_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX7465249.1"
                     /translation="MATALFTHRDCVLHEMGAGHPESPQRLKAVLAALEASGLAARLK
                     MREAPEAGREHLERVHDSAHVDLIFDSAPAAGYAYLDPDTSMNAKSLSAALRAAGAVV
                     SATDMVMSGEARNAFCAVRPPGHHATPGRPMGFCIFNNVAIGAIHALEAHGLERVAIL
                     DFDVHHGNGTEDAFHEDPRVMLCSTFQHPYYPYCGADSGNEHIINVPLPAMTDGRGFR
                     EAVERHWLPALEDFSPQMVFVSAGFDAHRDDPLAYLKLGDEDYRWITEKLVDVAERHA
                     EGRVVSTLEGGYNTDALGRCVVEHVRVLAG"
     gene            complement(1113..1607)
                     /locus_tag="VF309_01315"
     CDS             complement(1113..1607)
                     /locus_tag="VF309_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465250.1"
                     /translation="MIRKTIAAMLVALALPAAAQSVPAANYSDIWWNPTESGWGVTFT
                     QHPTSNQVFAVWYTYDPRAADASSPGNFKPLWLVMPGGQWTTPTHLTGDVYVTVGTPF
                     AQNWDPNAITNTKVGTFQFDFSSNSSGTFAYNIAAPAGLASNAAGFGMPSFTGTKQIQ
                     RQPF"
     gene            1836..>2355
                     /locus_tag="VF309_01320"
     CDS             1836..>2355
                     /locus_tag="VF309_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7465251.1"
                     /translation="MAGQVILGKERQIRLAMACILARGHLLIEDVPGVGKTTLSHALA
                     RLLGLDYQRIQFTSDLLPADIIGVSIFDRDSSTFRFHAGPIFAQIILADEINRASPKA
                     QSALLEAMEEHQVTAEGETRKLPEPFFVIATQNPMHQVGTFPLPESQLDRFLMRINLG
                     YPDAKAERELLKG"
BASE COUNT          403 a          765 c          781 g          406 t
ORIGIN      
        1 cgcgctcatc gaattcatcg cggaaaaact cggcatcgcc aggcgcgacg tgaccctcgt
       61 gagtggagcg aagtcgcgcg agaagcgctt cgcggtccgt ggatccggcg cgaaccccga
      121 tctcttgctg aagaactaaa gcgtattcac caatcgcctt ggggaaggcg aagcgtgaat
      181 cagcccgcga ggaccctcac gtgctcgacc acgcagcgtc cgagggcgtc ggtgttgtag
      241 ccgccctcga gcgtcgagac gacccggccc tcggcatggc gctcggccac gtcgaccagc
      301 ttctccgtga tccagcgata gtcctcgtcg ccgagtttca ggtaggcgag cggatcgtcg
      361 cggtgcgcgt cgaatcccgc ggatacgaac accatctgcg ggctgaaatc ctcgagcgcg
      421 ggaagccagt ggcgctccac ggcctcgcgg aatccgcgcc cgtcggtcat cgccggcagc
      481 ggcacgttga tgatgtgctc gttgccggaa tcggcgccgc aatacgggta atacggatgc
      541 tggaacgtgg agcacagcat cacgcgcgga tcctcgtgga aggcgtcctc ggtgccgttg
      601 ccgtggtgca cgtcgaagtc gaggatggcg acgcgctcga gcccgtgcgc ttcgagcgca
      661 tggatcgcgc cgatcgccac gttgttgaag atgcaaaagc ccatcggacg cccgggcgtg
      721 gcgtgatggc ctggggggcg caccgcgcag aaggcgttgc gcgcctcgcc cgacatcacc
      781 atgtcggtgg cgctcaccac cgcgcccgcg gcgcgcagcg cggccgagag cgatttcgcg
      841 ttcatcgaag tgtcgggatc gaggtaagcg tagccggcgg cgggcgccga atcgaagatg
      901 aggtccacgt gcgcgctgtc gtggaccctc tccaggtgct cacgaccggc ctcgggcgcc
      961 tcgcgcatct tgagccgcgc cgcgaggccc gaggcctcga gcgcggcgag caccgccttc
     1021 aggcgctgcg gcgattcggg atgccccgcg cccatctcgt gcaggacgca gtcccgatgg
     1081 gtgaatagcg cggtggccat gccgcgatgg tactagaacg gctggcgctg gatctgcttg
     1141 gtccctgtga aggaaggcat gccgaaaccg gcagcattgc tggcgagccc ggccggcgcg
     1201 gcgatgttgt acgcgaacgt gccggagctg ttggaggaga aatcgaactg gaacgtgcca
     1261 accttggtgt tcgtaatcgc gttgggatcc caattttgcg cgaacggcgt gcccacggtc
     1321 acgtagacgt ctccggtgag gtgcgtgggc gtggtccact ggccaccggg catcaccagc
     1381 cagaggggct tgaagttgcc cggcgagctg gcatccgcgg cgcgcggatc gtacgtgtac
     1441 cagaccgcga atacctggtt gctggtgggg tgctgcgtga aggtgacgcc ccagccggac
     1501 tcggtggggt tccaccagat gtcggagtag ttggcggcgg gcacgctctg cgccgcggcc
     1561 ggcagggcca gcgcgacgag cattgcggcg atcgttttgc gaatcatcgt gtctctttca
     1621 tgttcatgca ggcatcgaag ggtacggcta ttgtgggtaa acagtttcgc aaactcaacc
     1681 ttccaatgcc gggcaccgcg tctcaaaacg gcgacgtgct atcctcgtca cgcatacccg
     1741 attgactcgt aaaccgctga caaggttccc ttttttccat gcttctcgat gcgcgcagcc
     1801 ggcttgccgc cagccccacc gaagcggtca tcgcgatggc cggacaggtc atcctcggca
     1861 aggagcgcca gatccggctc gccatggcgt gcatcctcgc gcgcggccac ctgctgatcg
     1921 aggacgtgcc gggcgtcggc aagacgacgc tctcgcacgc gctggcgcgc ctcctggggc
     1981 tcgactacca gcgtatccag ttcacctccg acctcctgcc cgccgacatc atcggcgtgt
     2041 cgatcttcga tcgcgacagc tcgactttcc gcttccacgc gggcccgatc ttcgcgcaga
     2101 tcatcctcgc cgacgagatc aaccgcgcga gcccgaaggc gcaaagcgcg cttctcgagg
     2161 ccatggagga gcaccaggtg accgcggagg gcgagacgcg caagcttccc gaacccttct
     2221 tcgtgatcgc gacgcagaat ccgatgcacc aggtcgggac ctttcccctt ccggaatccc
     2281 agctcgatcg ctttctcatg cgcatcaacc tgggctatcc cgacgcgaaa gccgagcgcg
     2341 agctgctgaa gggcg
//
LOCUS       DASVII010000100         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_400580,
            whole genome shotgun sequence.
ACCESSION   DASVII010000100 DASVII010000000
VERSION     DASVII010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400580"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF309_01325"
     CDS             <1..789
                     /locus_tag="VF309_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7465252.1"
                     /translation="IRVMHWINVVSATILLLSGLQIFNAHPTLYWGKSSYTGNPPILA
                     LTAKEQGDDVIGITRVFGHDFVTTGLLGASANSEGEIVARGFPSWMTIPDSAWLAMAR
                     AWHLFFAWVFAINGLAYIAYSIATRHLTRDLAPTRREWRSIGQSIKDHLRFRHPKGEA
                     ARDYNVLQKLAYLAIVFIVLPLLVLMGLGMSPWLDALVPNWIDLFGGRQSARTLHFAA
                     AWILVAFVLIHVFEVVVTGLWNNLRSMITGRYRVEAQASHEKPE"
     gene            773..1543
                     /locus_tag="VF309_01330"
     CDS             773..1543
                     /locus_tag="VF309_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7465253.1"
                     /translation="MKSPNERRRFVLRAAAGFAALVTAGCERLSRSEWFPKVLAVGES
                     LSSGAQHLISPRKSMAQEFSEADLSPQFRSNGTSMPNDPGYQALAANAFAGWALQVDG
                     LVANPLKLTLAELRTMPARTQITRHDCVEGWSAIGKWTGVPLHEVLALAKPQPNARFA
                     VFHCADPMEEDGTHKYYESVDMDDAYHPQTILAYDLNGATLPVANGAPIRLRVERQLG
                     YKMAKYVMRIELVESFAHIAGGKGGYWEDEGYEWYAGI"
     gene            1644..1820
                     /locus_tag="VF309_01335"
     CDS             1644..1820
                     /locus_tag="VF309_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7465254.1"
                     /translation="MNKDQVKGRAKEVAGKIEKNVGRAVGSTKTEAKGLVKEVAGKIQ
                     KAVGDARNREFDKP"
BASE COUNT          367 a          674 c          671 g          325 t
ORIGIN      
        1 atccgcgtca tgcactggat caacgtcgtc tcggcgacca tcctgctgtt gagcgggctg
       61 cagattttca acgcgcaccc gaccctctac tggggcaagt cgtcgtacac cggaaacccg
      121 ccgatcctcg ccctcaccgc gaaggagcag ggcgatgacg tcatcggtat cacgcgcgtc
      181 ttcggccacg acttcgtcac caccggcttg ctcggcgcct cggcgaattc cgagggcgag
      241 atcgtcgcgc gcggcttccc ctcgtggatg acgatccccg attcggcgtg gctcgcgatg
      301 gcgcgcgcgt ggcatctgtt cttcgcgtgg gtcttcgcga tcaacggcct cgcctacatc
      361 gcctactcga tcgccacgcg gcacctcacg cgcgacctcg cgccgacgcg gcgcgagtgg
      421 cgctcgatcg ggcaatcgat caaggaccac ctgcgctttc gccatccgaa gggcgaagcg
      481 gccagggact acaacgtcct gcagaagctc gcgtatctcg cgatcgtctt cattgtgctg
      541 ccgctcctcg tcctcatggg attggggatg tctccgtggc tcgatgcgct ggttccgaat
      601 tggatcgatc tcttcggtgg ccggcagtcg gcgcgcacgc tgcatttcgc ggcggcgtgg
      661 atcctcgtcg cgttcgtgct gatccatgtg ttcgaagtcg tcgtcacggg cctctggaac
      721 aacctgcgct cgatgatcac gggccgctat cgcgtggagg cgcaggcgag tcatgaaaag
      781 cccgaatgag cgccggcggt tcgtgctgcg cgccgccgcg ggattcgccg ccctggtgac
      841 ggcgggttgc gagcggctct cgcgaagcga gtggtttccg aaggtcctgg ccgtcggcga
      901 atcgctgagc agcggcgcgc aacacctcat cagcccgcgc aagtccatgg cgcaggaatt
      961 ctccgaagcc gacctttcgc cgcagttccg cagcaacggc acatcgatgc cgaatgatcc
     1021 ggggtaccag gcgctcgcgg cgaacgcatt cgccggctgg gcgctgcaag tagacgggct
     1081 cgtcgccaat ccgctgaagc tcacgctcgc ggagctgcgc acgatgcccg cacgcacgca
     1141 gatcacgcgc cacgattgcg tcgagggctg gagcgccatc ggcaaatgga ccggcgtgcc
     1201 gctccacgag gttctcgcgc tcgcgaagcc tcaaccgaac gcgcgcttcg ccgtgttcca
     1261 ctgcgccgat ccgatggaag aggacggcac ccacaagtac tacgagagcg tcgacatgga
     1321 cgatgcgtac catccgcaga cgatcctcgc ctacgacctg aacggcgcga cgctgcccgt
     1381 cgcgaacggc gctccgatcc gcctgcgcgt ggagcgccag ctcggctaca agatggccaa
     1441 gtacgtgatg cgcatcgagc tggtggaaag cttcgcgcac atcgccggag gcaagggcgg
     1501 ctactgggaa gacgaaggct acgagtggta cgcggggatc tgagcgggca gtcggtaggc
     1561 gccctcccac gttcgcgcgc gccggcgtcc gatggacggc aacgcgggcc acgcccattg
     1621 ttgctttttc aaaggagaac gcgatgaaca aggaccaggt gaagggccgc gcgaaagaag
     1681 tcgccggcaa gatcgagaag aacgtcggcc gcgcggtggg cagcaccaag accgaagcca
     1741 agggcctcgt gaaggaagtg gcgggaaaga tccagaaagc cgtcggcgac gcgcgcaacc
     1801 gcgagttcga caagccgtaa ctcttacggc cccggtgctc gaagggccat gcgccagcgc
     1861 atcggcccga ctagcgaacc acgcgcgacg gctccgagcc gtgctcgatg aacgtccgga
     1921 ccgccatgtc gaggcacgag aactggtagc aatcgacgac gcgccgcgca tcggtcggcg
     1981 cgatgagccc catgccgtac tgccccgtga gggacgagcg gaatagcgcg acgcggc
//
LOCUS       DASVII010000101         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_256783,
            whole genome shotgun sequence.
ACCESSION   DASVII010000101 DASVII010000000
VERSION     DASVII010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256783"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1867)
                     /locus_tag="VF309_01340"
     CDS             complement(194..1867)
                     /locus_tag="VF309_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7465255.1"
                     /translation="MNALLFLAAGYAWYAIARLNDQLAATIMLQGDIETANDFARRAQ
                     LDFKKQVQEWKDILLRGRDRDLYDKHLKAFTERSARVTQDLASLNSQVPTIGLAPTFA
                     DKAIAEHDELDRRYLEALDSYRAADASSALEVDKLVRGIDRAATDHIDELVKAVNNKG
                     EALGAQTARDAASEKHMLILGLALLAFLAATVSTIVGTITVLAITRRLRRATEFARTV
                     AAGNLGAPIEAGRQDELGQLLTSMRDMNDSLAGIVDRVRQAAEMVTTASSQIAAGNVD
                     LSSRTEEQASSLEETAASIEEMTATVNQNAQNAAEASGVATCAADVALRGGKAVDQVV
                     KMMENIQASSRKIADIIGVIDSIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNL
                     AQRSAEAAREIKSLITDSVSRVDTGAKLADGAGQTMTELVASVNQVSRIIGEIAAATK
                     EQSSGIAQVNVAITDLDKATQQNASLVEESTAASESLKGLAREMADAVSVFQLAGRAP
                     SLAAQGQGSVAAPKAVTQRKVAALPRKSRPMNAAALPVPAYGDEVWKEF"
BASE COUNT          252 a          592 c          699 g          341 t
ORIGIN      
        1 cttggcggcg tcctgggtca ccaggcggtc gatgatcgcg ccgagctccg agagcctcca
       61 caggcccgcg agcgcgcttg cgttgaccag gatgaagacc gcgagaaatc ccgcggtgag
      121 gcggccgcgg attccgaggg atttgaaggg attcatggag atctttcgtc gggtgtggag
      181 cggggttcgg tgcctagaat tctttccaga cttcgtctcc gtacgcgggg acggggagtg
      241 cggccgcgtt catcgggcgg gacttgcgcg gaagggccgc gacctttctc tgcgtgacgg
      301 ctttgggcgc ggcgaccgat ccttgtcctt gcgccgcgag cgagggtgcg cgcccggcga
      361 gctggaagac cgagaccgcg tcggccattt cgcgggcgag gcccttgagg gactcggagg
      421 cggcggtgga ctcctcgacg agcgaggcgt tctgctgcgt ggccttgtcc aggtcggtga
      481 tggcgacgtt cacctgggcg atgccggagc tctgctcctt ggtggccgcg gcgatctcgc
      541 cgatgatgcg cgagacctgg ttcacgctgg cgaccagctc ggtcatggtc tggcccgcgc
      601 cgtcggcgag cttcgcgccg gtgtcgacgc ggctgacgga atcggtgatg agggacttga
      661 tctcccgggc cgcttcggcg gagcgctgcg cgaggttgcg cacttcgccg gcgaccaccg
      721 cgaagccgcg gccctgctcg ccggcgcgcg ccgcttccac ggcggcgttg agcgccagga
      781 tgttggtctg gaaggcgatg gagtcgatga ccccgatgat gtcggcgatc ttgcgcgagc
      841 tcgcctggat gttttccatc atcttgacga cctggtcgac ggctttgccg ccgcgcagcg
      901 cgacatcggc ggcgcaggtg gcgaccccgc tcgcctcggc ggcgttctgg gcgttctggt
      961 tgacggtggc ggtcatctcc tcgatggaag cggcggtctc ctcgaggctc gaggcctgct
     1021 cctcggtgcg cgaggacagg tccacgttgc ccgcggcgat ctgcgaagac gccgtggtca
     1081 ccatctcggc ggcctgccgc acgcgatcga cgatgcccgc gagggaatcg ttcatgtcgc
     1141 gcatcgacgt gagcagctgt cccagctcgt cctggcgccc ggcctcgatc ggcgcgccga
     1201 ggttgcccgc ggcgacggtg cgcgcgaatt cggtggcgcg ccgcaggcgg cgcgtgatgg
     1261 cgaggaccgt gatggtgccc acgatcgtgc tcaccgtggc ggcgaggaac gcgaggagcg
     1321 cgagacccag gatgagcatg tgcttctcgg acgcggcgtc gcgggcggtc tgtgcgccca
     1381 gcgcttcacc cttgttgttg accgccttca cgagctcgtc gatgtggtcg gtcgcggcgc
     1441 ggtcgatgcc gcgcaccagc ttgtcgacct cgagtgcgct ggatgcgtcg gcggcgcggt
     1501 acgagtcgag agcctcgagg tacctgcggt cgagctcgtc gtgctcggcg atcgccttgt
     1561 cggcgaacgt cggcgcgagc ccgattgtcg gaacctgcga attcagggag gcgaggtcct
     1621 gggtcacccg cgccgagcgc tccgtgaaag ccttgaggtg cttgtcgtag agatcgcgat
     1681 cccggccacg gaggaggatg tccttccact cctggacctg cttcttgaaa tcgagctgcg
     1741 cgcgccgcgc gaaatcgttg gccgtctcga tgtcgccctg caacatgatc gtggccgcaa
     1801 gctggtcgtt cagccgggcg atggcgtacc acgcgtagcc ggccgccagg aagagcagcg
     1861 cgttcatgaa cacgagcagc gcgg
//
LOCUS       DASVII010000102         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_400602,
            whole genome shotgun sequence.
ACCESSION   DASVII010000102 DASVII010000000
VERSION     DASVII010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400602"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF309_01345"
     CDS             complement(<1..391)
                     /locus_tag="VF309_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465256.1"
                     /translation="MARNTKTLYEVLGVPRDAKVTDIGRAYNRIRADQAKEAAAPDAR
                     LLLAAKAAHDTLSDPDRRDEYDRSLADARFTAKHRKGAVVAASLAGVLAVGAGYYFVS
                     RSAGPAVEKPLGPIELLAAVSPQIGRVQ"
     gene            complement(455..1393)
                     /locus_tag="VF309_01350"
     CDS             complement(455..1393)
                     /locus_tag="VF309_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7465257.1"
                     /translation="MLIATLCVPLAASVPSRCEDAVAPIGKQRAAGSANEWDVRDVNH
                     PLMGPIRAAVQRRSVVTTAGSNKVLSLAFVSCQKSTGKIAIELANAAEGDARGGLGPV
                     DLPRLVCNSPRPQGDGVLAKSDLAASWEISTLGDALARGLSPAALRKCASIDVLQNVA
                     LPPASGVKSQKIAMELTPYGKELDAVFTACGEATAFASEARPAPALPQVARIEPPPRL
                     DPPPRAEPAPKVEASPTSDPGTQSGDARWRPARTIAKGRTNVRAGDSLNSAVVTQLPP
                     GVRILVQETSAEWWKVKSGGRASFSGYIRRDRVVIE"
     gene            complement(1509..>1692)
                     /locus_tag="VF309_01355"
     CDS             complement(1509..>1692)
                     /locus_tag="VF309_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HEX7465258.1"
                     /translation="LAFGGTVTTNGANTITVPAACAALAGGGSTGIPALSEWAMAMLA
                     ALLATAGFAAMRGRAR"
BASE COUNT          232 a          576 c          598 g          286 t
ORIGIN      
        1 tctgcacgcg gccgatctgc ggcgatacgg ctgccagcag ctcgatggga cccaggggct
       61 tctcgaccgc ggggcccgcc gaacgcgaga cgaagtagta ccccgccccg accgcgagca
      121 ccccggcgag cgacgccgcg acgacggctc ccttgcgatg tttcgcggta aagcgcgcgt
      181 ccgcgagcga gcgatcatat tcgtcgcgcc ggtcgggatc cgaaagcgtg tcgtgggctg
      241 ccttcgccgc caggaggaga cgggcgtccg gcgccgcggc ttccttggcc tggtcggcgc
      301 ggatgcggtt gtatgcgcgg ccgatgtccg tgaccttggc gtcgcgcggg actccgagga
      361 cttcgtaaag ggtcttggtg tttcgagcca tggctagcga agtcccctcc ctcggggaag
      421 ttcgagacga tggtatggca ttccgctgcc gcggtcactc gatgacgacg cgatccctgc
      481 ggatgtagcc gctgaaggat gcgcggccgc ccgatttcac cttccaccat tccgccgagg
      541 tctcctggac cagtatcctc acgcctggcg gcagctgggt gacgacggcg gaattgaggc
      601 tgtcgccggc gcgcacgttg gtcctgccct tcgctatggt gcgagccggc ctccaccggg
      661 catcgcccga ctgggtgcct ggatcgctcg ttggtgaagc ctcgactttc ggcgcgggct
      721 cggctctcgg cggagggtcg agtctcggtg gtggctcgat cctcgcgacc tgtggcaacg
      781 cgggcgcggg ccgcgcctcg gacgcgaacg cagtcgcctc gccgcacgcc gtgaataccg
      841 catcgagctc cttgccgtac ggcgtgagct ccatcgcgat cttctggctc ttcacgcccg
      901 acgccggcgg caacgcgacg ttctgcagga cgtcgatgga ggcgcacttg cgcagcgccg
      961 cgggcgacag gccgcgcgcc agcgcgtcac cgagggtgct gatctcccag cttgcggcga
     1021 ggtcgctctt cgcgaggacg ccgtcgcctt gcggccgcgg gctgttgcag acgaggcgcg
     1081 gcaggtccac gggcccgagg ccgccgcgag catcgccctc ggccgcgttg gccagctcga
     1141 tggcgatttt cccggtgctc ttctggcagg agacgaacgc gagcgagagg accttgttgc
     1201 ttcccgccgt ggtgacgacg gagcgtctct gcaccgcggc cctgatgggt cccatgagcg
     1261 gatggttcac gtcgcgcaca tcccattcgt tcgccgatcc ggcggcgcgc tgcttcccga
     1321 tcggcgcgac ggcgtcttcg cagcgcgagg gaacgcttgc cgcgaggggc acgcacagcg
     1381 tcgcaatgag caaggctctt cgcaatggtc gtcggcgcca gcgcgctagg cccttgctcc
     1441 tggcagcgcc tggcactgcg ccttcgaatg cagatggatt cggcgccgga tcgaagccgt
     1501 cgcgatgact accttgcgcg tccgcgcatc gcggcgaagc ccgcggtagc caggagcgcg
     1561 gccagcatgg ccatcgccca ctcggagagt gcggggatgc ccgtgctgcc tccgcccgcg
     1621 agcgcggcgc aagcagcggg gacagtaatt gtgttcgcac cattcgtcgt gacggttccg
     1681 ccgaaggcca gg
//
LOCUS       DASVII010000103         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_113072,
            whole genome shotgun sequence.
ACCESSION   DASVII010000103 DASVII010000000
VERSION     DASVII010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113072"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /gene="xseA"
                     /locus_tag="VF309_01360"
     CDS             <1..1316
                     /gene="xseA"
                     /locus_tag="VF309_01360"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7465259.1"
                     /translation="APPVLTVAELNRRVRAVLENQFEIVWVAGELSNVKKAPSGHWYF
                     CLKDPGAQIDCAMFRTRAQFLDFRPENGLQVEVRARVTLYEARGSYQLVVEEIRKAGL
                     GALYEAFGKLKAKLQAEGLFEADRKRALPSFPRAIGIVTSPAAAALRDVLTTLARRAS
                     MVPVILYPAQVQGEAAAGQIARAIEIANSRAEVDVLIVCRGGGSIEDLWAFNEEIVAR
                     AIAASSLPVVSGVGHETDFSIADFVADLRAPTPTAAAAAASPDREGLSGQVAALGRRL
                     ARDMRRIVDVGTQRLDSAARRLLTPAERLARDRERIEQCVRRLRMTSAAALNLRRLAL
                     GALAPRLRRALPDVEGERRGAARMRERLGAVLTRNLRERERRLGALRTALAHLDPTQV
                     LGRGYSIVRDPDGHVRTASAGLAGGDALDITFARGGAAVSVKERR"
     gene            1369..1614
                     /locus_tag="VF309_01365"
     CDS             1369..1614
                     /locus_tag="VF309_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX7465260.1"
                     /translation="MAKKGKSSKAKSDNAVYKVIEIVGTSSQSWADAAANACKTASKS
                     LRDLRVAEVDKLDVKIDGTKLIYRAKLKVSFKFHGED"
     gene            complement(1599..>2275)
                     /gene="miaA"
                     /locus_tag="VF309_01370"
     CDS             complement(1599..>2275)
                     /gene="miaA"
                     /locus_tag="VF309_01370"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7465261.1"
                     /translation="RGATPLFAGGTMLYFRSLATGLADLPDAQPAIRAGIEESALRMG
                     WPALHAELAKVDPVTAARIDPADAQRIQRALEVYRHSGKVLSAFHAEARDAAPALDAL
                     ALALEPSDRAVLHERIAERFRGMLARGLVEELEGLRARHHLTDRLPAMRAVGYRQAWD
                     VLEGRAPAAALEARGTAATRQLAKRQLTWLRSMEGLDRLDCLRPDLADAAAERVARFL
                     ETQSSP"
BASE COUNT          335 a          792 c          832 g          316 t
ORIGIN      
        1 cggcaccccc ggtcctcacc gtcgcggagc tgaaccgccg ggtccgcgcc gtgctcgaga
       61 accaattcga gatcgtgtgg gtcgcgggcg agctctccaa cgtgaagaag gcgccttccg
      121 ggcattggta cttctgcctc aaggatcccg gcgcgcagat cgattgcgcg atgttcagga
      181 cgcgcgccca gttcctcgac ttccgtcccg agaatgggct gcaggtcgag gttcgcgccc
      241 gggtcacgtt gtacgaggcg cgcggaagct accagctcgt cgtcgaggag atccgcaagg
      301 cgggacttgg cgcgctctac gaggccttcg gcaagctcaa ggcgaagctc caggccgagg
      361 gcctcttcga ggccgatcgc aagcgggcgc tgccgtcctt tccccgcgcg atcggcatcg
      421 tgacgtcgcc cgccgccgcc gcgctgcgcg acgtgctgac gaccctcgcg cggcgggcga
      481 gcatggtgcc ggtgatcctc tatcccgccc aggtgcaggg cgaagcggcc gcgggccaga
      541 tcgcgcgcgc gatcgaaatc gccaattcgc gcgcggaagt cgacgtgctg atcgtctgcc
      601 gcggcggcgg aagcatcgag gacctttggg cgttcaacga ggagatcgtc gcgcgcgcca
      661 tcgcggcgtc ttcccttccc gtcgtgagcg gtgtcggcca cgagaccgat ttctccatcg
      721 cggacttcgt cgccgacctg cgcgcgccta cgcccacggc cgcggcggca gcggcgagcc
      781 cggaccgcga gggcctttcc gggcaggtgg cggccctggg ccggcgcctg gctcgcgaca
      841 tgaggcgaat cgtcgatgtc gggacgcagc gcctcgatag cgccgcgcgg cgcctcctta
      901 cgccggccga gcgcctcgcg cgcgatcgtg agcggatcga gcagtgcgtg cggcgcctgc
      961 gcatgacgag cgccgccgcg ctgaacctgc gccggctcgc cctgggtgcg ctcgcccccc
     1021 ggctgcgccg ggcgctaccc gacgtcgagg gcgagcggcg cggcgcggcg cgcatgcgtg
     1081 agcggctggg cgccgtgctc acgcgcaacc tgcgcgagcg cgaaaggcgc ctcggcgcgt
     1141 tgcgcaccgc gctcgcgcac ctcgatccca cgcaggtgct gggccgcggc tacagcatcg
     1201 tccgcgaccc cgatgggcac gtgcgcacgg cgagcgcggg actcgccggc ggcgatgcgc
     1261 tcgacatcac gttcgcccgg ggcggcgccg cggtttctgt gaaagagcgc cgctaggttt
     1321 atattgcggt ttcaattggg acaccgatcc acaccacggg ggatagccat ggcgaagaag
     1381 ggcaagagca gcaaggcaaa gagcgacaac gccgtctaca aggtgatcga gatcgtcggc
     1441 acgagctcgc agtcgtgggc cgatgccgcg gccaatgcgt gcaagacggc ctcgaaaagc
     1501 ctgcgcgacc ttcgcgtggc ggaagtcgac aagctcgacg tgaagatcga cggcaccaag
     1561 ctcatctatc gcgcgaagct caaggtgagc ttcaagtttc acggcgagga ctgagtctcg
     1621 aggaatcgcg cgacgcgctc ggccgccgcg tccgcgagat ccggcctcag gcagtcgagg
     1681 cggtcgagcc cttccatcga gcgtagccag gtgagctggc gcttggcgag ctggcgcgtc
     1741 gcggccgtgc cgcgcgcctc cagcgcagcg gccggcgcgc ggccttcgag gacatcccac
     1801 gcctggcgat agcccaccgc gcgcatcgcg ggcaagcggt cggtcaggtg gtggcgagcg
     1861 cgcagtccct cgagctcctc gacgagcccg cgcgcgagca tgccgcggaa acgctcggcg
     1921 atgcgctcgt gcaggaccgc gcgatccgag ggctcgagcg cgagcgccag ggcatcgagc
     1981 gccggcgcgg cgtcgcgcgc ttcggcgtgg aacgccgaga gcaccttgcc cgagtggcgg
     2041 taaacctcga gcgcgcgctg gatgcgctgc gcgtcggcgg ggtcgatgcg cgcggccgtg
     2101 accggatcga ccttcgcaag ctcggcgtgc agcgccggcc agcccatgcg cagcgcgctt
     2161 tcctcgatcc ccgcgcggat cgcgggctgc gcgtcgggca agtccgcgag cccggtcgcg
     2221 agcgagcgga agtagagcat cgtgcctccg gcgaagaggg gcgtggcccc gcgcg
//
LOCUS       DASVII010000104         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_652271,
            whole genome shotgun sequence.
ACCESSION   DASVII010000104 DASVII010000000
VERSION     DASVII010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652271"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF309_01375"
     CDS             complement(<1..239)
                     /locus_tag="VF309_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX7465262.1"
                     /translation="MIARALLASVALACAVAAIPAAAQKAPAAVVDVRLADLPLEARQ
                     TVALIHKAGPYPYARDGAVFVNREGALPRQKRGYYR"
     gene            complement(236..658)
                     /locus_tag="VF309_01380"
     CDS             complement(236..658)
                     /locus_tag="VF309_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7465263.1"
                     /translation="MRAGLEGTFRYRVPESKTVPGIYAEAADFQMMPRVLATGYLVAL
                     AEWACIELIKPHLDWPREQSLGTRVELSHLAATPPGFTVEIRARVESVAGRKLVFAVS
                     ASDGADLISEGTHERHVIDARRFEEKIAAKRARAEAAP"
     gene            complement(672..1103)
                     /gene="fur"
                     /locus_tag="VF309_01385"
     CDS             complement(672..1103)
                     /gene="fur"
                     /locus_tag="VF309_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEX7465264.1"
                     /translation="MSDPRELKDTGLKVTVPRLKVLDLFRHSTERHLTAEDVYRKLLD
                     EHSDVGLATVYRVLTQFEQAGILVRHHFEGGKAVYEMNEGGHHDHLVCLQCGRVEEFY
                     DSEIEKRQNKVAKERGFQIHDHQLYLYADCIKTHCAFRPKG"
     gene            1146..1526
                     /gene="bamE"
                     /locus_tag="VF309_01390"
     CDS             1146..1526
                     /gene="bamE"
                     /locus_tag="VF309_01390"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX7465265.1"
                     /translation="MRILAPIVAATLILAGCVYKIDIQQGNYVTQDVAAKLKTGMTKS
                     EVRLLLGTPLLVDPFHANRWDYYFANSKGGKAEPSSRLSIFFENDKVVNYVGEARPAL
                     PPPVGGTTPPAPAAGATTPTPAAK"
BASE COUNT          240 a          516 c          519 g          254 t
ORIGIN      
        1 cgatagtagc cgcgcttctg gcgcggcagc gcgccctcgc gattgacgaa gaccgcgccg
       61 tcgcgcgcgt acggataggg gccggccttg tggatgaggg cgaccgtctg gcgcgcttcg
      121 agcgggagat ccgcgagcct cacgtcgacc acggccgcgg gcgctttctg cgccgccgcg
      181 ggaatcgcgg ccacggcgca cgcgagggcc acgctcgcga gcaatgcgcg cgcgatcatg
      241 gagccgcctc ggcgcgcgcg cgcttggcgg cgatcttctc ctcgaagcga cgcgcgtcga
      301 tcacgtggcg ctcgtgggtg ccttcggaga tgagatcggc gccgtcgctc gcgctcacgg
      361 cgaacacgag cttgcgcccg gcgacggact cgacgcgcgc gcggatctcg acggtgaatc
      421 ccggcggcgt cgcggcgagg tgcgacagct ccacgcgcgt gccgagcgac tgctcgcgag
      481 gccagtcgag gtgcggcttg atgagctcga tgcacgccca ctcggcgagc gccaccaggt
      541 aacccgtggc gaggacgcga ggcatcatct ggaagtccgc ggcctcggcg tagatgccgg
      601 ggacggtctt ggactccggc acgcggtagc ggaaagtgcc ctcgaggccc gcacgcaatg
      661 tgtcctccac ctcagccctt gggccggaaa gcgcagtgcg tcttgatgca gtcggcgtag
      721 agatacagct ggtgatcgtg gatctggaag ccccgctcct tcgcgacctt gttctggcgc
      781 ttctcgatct cggaatcgta gaactcctcc acgcgcccgc actgcaggca gacgagatgg
      841 tcgtggtggc cgccctcgtt catctcgtag accgccttgc cgccctcgaa atggtggcgc
      901 acgaggattc ccgcctgctc gaactgcgtg agcacgcgat agaccgtggc gaggccgacg
      961 tcggagtgct cgtcgaggag cttcctgtag acgtcttcgg ccgtgaggtg gcgctcggtg
     1021 ctgtggcgga acaggtcgag caccttcagg cgcggtaccg tcaccttgag gccggtgtcc
     1081 ttgagttccc ggggatcgct catggcgtga attataatgc gcgcttcgct cccccttggt
     1141 atttcatgcg catcctcgct cccatcgtcg ccgcaacgct catcctcgcg ggctgcgtgt
     1201 acaagatcga catccagcaa ggcaactacg tgacccagga cgtggccgcc aagctgaaga
     1261 ccgggatgac caagtccgag gtccgcctgc ttctcgggac tccgctgctg gtcgacccat
     1321 tccacgcgaa tcgctgggac tactacttcg ccaactcgaa gggcgggaag gcggagccca
     1381 gctcgcgcct ctccatcttc ttcgagaacg acaaagtggt gaactacgtg ggcgaagcgc
     1441 gccccgcctt gccgccgccg gtcgggggca cgacgcctcc cgcgccggcc gcgggcgcga
     1501 cgacgccgac tccggcagcg aaatgaacc
//
LOCUS       DASVII010000105         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_400719,
            whole genome shotgun sequence.
ACCESSION   DASVII010000105 DASVII010000000
VERSION     DASVII010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400719"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..479
                     /locus_tag="VF309_01395"
     CDS             78..479
                     /locus_tag="VF309_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465266.1"
                     /translation="MRTIRTVATRAILLAAVVSAVPAAAQFGPIHVAVNRHEYVGRGC
                     PVEIVFTGTINIEPHDRGFVFNYHWERSDGAKSGTKVVRPGPNERTLVVRERWRVGGR
                     DKTYNVSQVLHVNSGNTHLTEGSPTVHVECR"
     gene            510..2066
                     /locus_tag="VF309_01400"
     CDS             510..2066
                     /locus_tag="VF309_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465267.1"
                     /translation="MHRLILSLVLALALPHEGFAKVFRYASQIDPGTMDPHGIASLYN
                     TRVVSQIYEVLVGRDEQFRIDPRLALSWSLVEPKVWRFKLRPGVKFHDGTPFTADDVV
                     FSVERALAPTSQQKQTLPNVTGARKVDALTVDILTSQPTPTLPLALTNLRIMSKAWCV
                     LHHIEKPLNFNAKEETFATRNTNGTGPFMLKSWDADVKTVLVANPAYWGQHGNVTEAQ
                     FLVVGSGATRLSGLVSGELDFVVDAGVQDVERLSRTPGIKIEQGEGLGAQYLSFDFAH
                     ATLQHGDAGGTNPFRNIKVREAMRLAIDLGAIQSKVMRNLSTIGRAIYSPKVDGWDAR
                     FAVPAPYDPERARALLKEAGYPNGFSVALDCSSQAPADAVGQAVAGMLARVGIRVAYQ
                     PVPFNVLLPKVTGHESSLYVIGWTTFTADAEGVLVPLAHTPSAPGTGDFNFGGYSNAN
                     VDSLIDRARVELDPVKRRALLIDAMAALDADIAFIPLVYRRVVWAMRKNVHTPILPND
                     VVDLRFVDID"
BASE COUNT          379 a          778 c          673 g          349 t
ORIGIN      
        1 ggaaaggttg gtgcaagcta cccggcccaa gatacttccc gccgctcgaa caaaccgtcc
       61 aacaaagagg aggccgcatg cgcacgatcc gcaccgtcgc gacaagagca atcctgctcg
      121 ccgccgtcgt ctccgccgtt cccgccgccg cccagttcgg cccgatccac gtcgccgtga
      181 atcgccacga atacgtgggg cgcggctgcc ccgtcgagat cgtctttacc gggaccatca
      241 acatcgagcc ccacgaccgg ggattcgtct tcaactatca ctgggagcgc agcgacggcg
      301 cgaagagcgg cacgaaagtc gtgcgccccg gccccaacga gcggaccctc gtcgtgcgcg
      361 aaagatggcg cgtcggcggt cgcgacaaga cgtataacgt ctcccaggtg ctgcacgtga
      421 attccggcaa cacgcacctc accgaaggct cgcccaccgt gcacgtggag tgccgctgaa
      481 acatggggcg actcgctagg gtcgccccga tgcaccgcct catcctttcg ctggtcctcg
      541 ccctcgcgct tccgcacgag ggcttcgcga aggttttccg ctacgcctcg cagatcgatc
      601 cggggaccat ggatcctcac gggatcgcga gcctgtacaa cacgcgcgtg gtctcgcaaa
      661 tctacgaggt gctggtgggg cgcgacgagc agttccgcat cgacccgcgc ctcgcgctgt
      721 cgtggtcgct cgtggagccg aaggtgtggc gcttcaagct gcgccccggc gtgaagttcc
      781 acgacggcac gcccttcacc gcggacgacg tggtcttcag cgtcgagcgc gcgctcgcac
      841 ccacctcgca gcagaagcag acactgccca acgtcaccgg cgcgcgcaag gtcgatgcgc
      901 tcaccgtgga tatcctcacg tcgcagccca cgccgaccct gccgttggcg ctcaccaacc
      961 tgcggatcat gagcaaggcc tggtgcgtgc tgcaccacat cgagaagccg ctcaacttca
     1021 acgcgaagga ggagaccttc gccacgcgca acaccaacgg caccgggccc ttcatgctca
     1081 agagctggga cgcggacgtg aagacggtgc tcgtcgccaa ccccgcctat tggggccagc
     1141 acggcaacgt gaccgaggcg cagttcctcg tggtgggctc gggcgcgacg cggctctcgg
     1201 ggctcgtctc cggcgagctc gacttcgtcg tcgacgcggg cgtgcaagac gtcgagcgcc
     1261 tgtcgcgcac gcccggcatc aagatcgagc agggcgaggg gctcggcgcg cagtacctca
     1321 gcttcgactt cgcgcacgcg acgctgcagc atggcgatgc gggcggcacg aaccccttcc
     1381 gcaacatcaa ggtgcgcgag gcgatgcgcc tggcgatcga cctgggcgcc atccagtcga
     1441 aggtgatgcg caacctgagc accatcgggc gtgccatcta ttcgcccaag gtcgacggct
     1501 gggatgcgcg cttcgcggtg cccgcgccct acgatcccga acgcgcgcgc gccctgttaa
     1561 aggaggcggg ctaccccaat ggcttctcgg tcgcgctcga ttgcagctcc caggcgccgg
     1621 ccgacgcggt gggccaggcg gtggccggca tgctcgcgcg cgtgggcatt cgcgtcgcct
     1681 accagcccgt gcccttcaac gtcctgctgc cgaaggtcac cgggcacgag tcgagcctgt
     1741 acgtgatcgg ctggacgacg ttcaccgccg atgccgaggg cgtgctcgtg ccgctcgcgc
     1801 atacgcccag cgcgcccggc accggcgact tcaacttcgg cggctactcc aacgcgaacg
     1861 tcgattcgct gatcgatcgc gcgcgcgtgg agctcgaccc cgtcaagcgg cgcgcgctgc
     1921 tgatcgacgc catggccgcg ctcgacgccg acatcgcctt catcccgctc gtctatcgcc
     1981 gcgtcgtgtg ggccatgcgc aagaacgtgc acacgcccat ccttcccaac gacgtcgtcg
     2041 acctacgctt cgtcgatatc gactagagaa ttcgtgtcag gtacgcattc ttgattcgta
     2101 cctgacacga attctcgtga aagtgcggca actcacaaag cgccccgctt tgttacaaga
     2161 accccccctc aggttacgg
//
LOCUS       DASVII010000106         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_472718,
            whole genome shotgun sequence.
ACCESSION   DASVII010000106 DASVII010000000
VERSION     DASVII010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472718"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VF309_01405"
     CDS             <1..957
                     /locus_tag="VF309_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465268.1"
                     /translation="GDLVMVNALLIQLYIPLNFLGVMYREIKQAVVDMEKMFRLLGEN
                     REVEDKPGAPALAVRGGAIRFENVDFSYDGKREILKNVSFEVAPGRKVAVVGTSGSGK
                     STLARLLFRFYDVNRGRITIDGQDVRDVTQSSVRAALGVVPQDTVLFNDTIYYNVSYG
                     RPGATRAEIENAARVAHIADFIARLPEGWETMVGERGLKLSGGEKQRVSIARTVLKNP
                     PILVFDEATSALDSNTEKSIQAELLEISRDRTTLVIAHRLSTIVDADEILVLEHGQIV
                     ERGNFRELITANGLFAEMWRLQQEEGVKEGTKSAEEAAQLVQ"
     gene            complement(925..>1629)
                     /locus_tag="VF309_01410"
     CDS             complement(925..>1629)
                     /locus_tag="VF309_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7465269.1"
                     /translation="LHGAAFDHSVWQWQSRYFAHHGFSVLALDLPGHGRSPGTMRATI
                     EEWAEWVAAFFDAASLSGVALVGHSMGSLIALETALKHGARLSKLALIGTAAPMPVGD
                     AFLAAALDRSPAALDMEAVWGHSRQAQLMQSPVPGMTLLGASRSLNERSRPGALHAAL
                     AACNAYRPSMEAVRALRVPTLVVAGKRDQMTQAKAGKALAAEIPGARLAMLDAGHSMM
                     SEAPRELLHELRGFLG"
BASE COUNT          275 a          558 c          557 g          239 t
ORIGIN      
        1 ggcgacctgg tgatggtgaa cgcgctcttg atccagctct acatcccgct caactttctc
       61 ggcgtgatgt accgcgagat caagcaggcg gtggtcgaca tggagaagat gttccgcctc
      121 ctcggcgaga accgcgaggt cgaggacaag ccgggcgcgc ccgcgctcgc ggtgcgcggc
      181 ggcgccatcc gcttcgagaa cgtcgatttc agctacgacg gcaagcgcga gatcctgaag
      241 aacgtgagct tcgaggtcgc gccggggagg aaggtcgcgg tggtgggcac gagcggctcg
      301 ggcaagtcga cgctcgcgcg cctgctcttc cgcttctatg acgtgaaccg cgggcgcatc
      361 acgatcgacg gccaggacgt gcgcgacgtg acccagtcga gcgtgcgcgc ggcactcggc
      421 gtcgtgcccc aggacaccgt gctcttcaac gacacgatct actacaacgt ctcctacggc
      481 cgccccggcg cgacgcgcgc ggaaatcgag aatgcggcgc gcgtcgcgca catcgccgat
      541 ttcatcgcgc ggcttccgga gggctgggag acgatggtcg gcgagagggg cctcaagctc
      601 tcgggcggcg agaagcagcg cgtctcgatc gcgcgcacgg tactgaagaa cccgccgatc
      661 ctggtcttcg acgaggccac gtccgcgctc gactcgaata ccgagaagtc catccaggcc
      721 gagcttctcg agatctcgcg cgaccgcacg acgctcgtca tcgcccatcg cctgtcgacc
      781 atcgtcgatg ccgacgaaat cctggtgctc gagcacggcc agatcgtgga gcgcggcaat
      841 ttccgcgagt tgatcaccgc gaacggcctc ttcgcggaga tgtggcgcct tcagcaggag
      901 gaaggcgtga aggaaggaac gaagtcagcc gaggaagccg cgcagctcgt gcagtagctc
      961 gcgcggcgcc tcactcatca tcgagtgccc ggcgtcgagc atcgcgagcc gcgcccccgg
     1021 aatctccgcg gcgagggcct tgcccgcctt cgcctgcgtc atctggtcgc gcttgcccgc
     1081 aaccaccagc gtcggcacgc gcagcgcgcg caccgcctcc atcgaaggcc ggtacgcatt
     1141 gcacgcggcg agcgccgcgt gcagcgcacc gggccgcgag cgctcgttca ggctgcggct
     1201 ggcgcccagc agcgtcatgc cgggcaccgg gctttgcatg agctgcgcct ggcgcgagtg
     1261 tccccacacc gcttccatgt cgagcgcggc gggagatcgg tcgagagccg cggcgaggaa
     1321 ggcatcgccc accggcatcg gcgcggcggt gccgatcaac gcgagcttcg acaggcgcgc
     1381 gccgtgcttc aacgcggtct cgagcgcgat gagcgagccc atgctgtggc ccacgagcgc
     1441 aacgccggag agactcgccg cgtcgaagaa ggccgccacc cactcggccc attcctcgat
     1501 ggtcgcgcgc atcgtgccgg ggctgcgtcc gtggccgggc aggtccagtg ccagcaccga
     1561 gaagccgtga tgcgcgaagt agcgcgattg ccattgccac acgctgtggt cgaacgccgc
     1621 gccatgcag
//
LOCUS       DASVII010000107         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_688581,
            whole genome shotgun sequence.
ACCESSION   DASVII010000107 DASVII010000000
VERSION     DASVII010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688581"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VF309_01415"
     CDS             <1..505
                     /locus_tag="VF309_01415"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7465270.1"
                     /translation="VVNMADMETLEVEADVSEASISKIKEGMPTEIQLDAYPDVRLLG
                     EVSRIVPTVDRTKATLLVKVKFVEKDARVLPDMSSKVAFLSRAPKPEERKPVTAVRPE
                     AVVKRGDRDVVYLVQEGNVVKEIAVGKPEKLGDLVQVSGVKAGDKVVLSPPERVRDAA
                     TVTLAKK"
     gene            510..1247
                     /locus_tag="VF309_01420"
     CDS             510..1247
                     /locus_tag="VF309_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465271.1"
                     /translation="MAERPIVSITHLSKSYRRGDQIVPVLQDITLSIPEGDFIALMGP
                     SGSGKSTLLNLIAGIDKPDAGELLVGGEDITKLGEADLADWRASNVGFIFQFYNLMPV
                     LDAFENVELPLLLTSLSKRDRRERVETALALVGLSDRMEHYPNELSGGQQQRVAIARA
                     LITDPAIIVADEPTGDLDRKSAEDVLGLLRRLNAEMGKTIVMVTHDGHAVQSARHVMH
                     LDKGELIPEKLVSETNLPGTHAGQIGL"
     gene            1265..2431
                     /locus_tag="VF309_01425"
     CDS             1265..2431
                     /locus_tag="VF309_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465272.1"
                     /translation="MYYLKLLFKNIFRHRLRSLLTMVGLVVAILAFGLLQTVVHAWYA
                     GADMASANRLITRNATSLVFVLPAYYRERIKAIDGVTSVAISNWFGGIYKDQSFANFF
                     ATFAVDHDNFFDLYPEFKVKPDELLAFKKDRQGAMVGRLIADQHGFKVGDVIPLTSPI
                     YGGGTWEFNVRGIYEPKDETTVTRQMYFHWDYLNEQMKKRFPRRAESAGVFVVQIGDG
                     GRAAEVSQAIDKEFRNSLGETLTETEKAFSLGFVAQTEAIVTAVRIVSFVVIVIIFAV
                     VANTMAMTARERLAEYATLKALGFHPGFVMALIMGESIMISALGGAIGIALTFPVAAG
                     FKSAMGSMFPVFYVTQETVLMQVAAAASVGILAGILPSIRAARVKIVDGLRFAG"
     gene            2428..>2609
                     /locus_tag="VF309_01430"
     CDS             2428..>2609
                     /locus_tag="VF309_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465273.1"
                     /translation="MKIPFSYIFRNLWTRKLTTLLTAGDMALVVFVFAAVLMLDAGLK
                     KTLVSTGSLDNAILLR"
BASE COUNT          460 a          865 c          850 g          434 t
ORIGIN      
        1 cgtggtcaac atggccgaca tggaaacgct ggaagtcgag gccgacgtgt cggaggcgtc
       61 gatctccaag atcaaggagg ggatgcccac cgagatccag ctcgacgcct atcccgacgt
      121 gcgcctgctg ggcgaagtct cgcgcatcgt gcccacggtc gaccgcacca aggcgaccct
      181 cctcgtgaag gtgaagttcg tcgagaagga tgcgcgcgtg ctgcccgaca tgagctcgaa
      241 ggtggcgttc ctgtcgcgcg ccccgaagcc cgaggagagg aagcccgtca ccgcggtgcg
      301 cccggaagcg gtggtgaagc gcggcgaccg cgacgtggtc tacctcgtcc aggagggaaa
      361 tgtcgtgaag gagatcgccg tgggcaagcc cgagaagctc ggcgacctcg tccaggtgag
      421 cggcgtgaag gccggggaca aggtggtcct ctccccgccc gagcgcgtgc gcgacgcggc
      481 gacggtcacg ctcgccaaga agtgacgcca tggccgagcg gccgatcgtg agcatcacgc
      541 acctgtcgaa gagctaccgc cgcggcgacc agatcgtgcc ggtgctgcag gacatcacgc
      601 tctcgattcc cgagggcgac ttcatcgcgc tcatgggtcc gtcgggctcg ggcaagtcca
      661 cgctcctgaa cctgatcgcg ggcatcgaca agcccgacgc gggcgagctg ctggtcggcg
      721 gcgaggacat caccaagctc ggcgaagccg acctcgccga ttggcgggcc tcgaacgtcg
      781 gattcatctt ccagttctac aacctgatgc cggtcctcga cgccttcgag aacgtggagc
      841 ttcccctgct gctcacctcg ctctcgaagc gcgatcgccg cgagcgggtc gagaccgcgc
      901 tcgcgctggt gggcctctcc gatcgcatgg agcactaccc caacgagctc tcgggagggc
      961 agcagcagcg cgtggccatc gcgcgcgccc tgatcaccga tcccgccatc atcgtcgccg
     1021 acgagcccac gggcgacctc gaccgcaaga gcgccgaaga cgtcctcggc ctcctgcgaa
     1081 ggctcaacgc cgagatgggc aagaccatcg tcatggtgac ccacgacggg cacgcggtgc
     1141 agtcggcgcg ccacgtcatg cacctcgaca agggcgagct gataccggaa aaactggtgt
     1201 cagagaccaa tttgcccggt acgcatgccg ggcaaattgg tctctgacac cagttttcaa
     1261 tctcatgtac tacctcaagc tcctcttcaa gaacatcttc cgccaccggc tgaggagcct
     1321 tctcaccatg gtcgggctgg tggtcgcgat cctcgcgttc gggctgctgc agacggtggt
     1381 gcacgcgtgg tacgcgggcg cggacatggc gagcgccaac cgcctcatca cgcgcaacgc
     1441 gacatccctc gtcttcgtgc tccccgcgta ctaccgcgag cgcatcaagg cgatcgacgg
     1501 cgtgacctcg gtcgcgatct ccaactggtt cggcgggatc tacaaggacc agtcgttcgc
     1561 gaacttcttc gcgaccttcg ccgtcgacca cgacaacttc ttcgacctct accccgagtt
     1621 caaggtgaag cccgacgagc tcctcgcctt caagaaggac cgccagggcg cgatggtggg
     1681 ccggctcatc gccgaccagc acggcttcaa ggtgggcgac gtgatcccgc tcacgagccc
     1741 catctacggc ggcggcacgt gggagttcaa cgtgcgcggc atctacgagc cgaaggacga
     1801 gaccaccgtc acgcgccaga tgtacttcca ctgggattac ctgaacgagc agatgaagaa
     1861 gcgcttcccg cgccgcgccg agtccgccgg cgtcttcgtc gtgcagatcg gcgacggcgg
     1921 ccgcgccgcc gaggtgagcc aggcgatcga caaggagttc cgcaattccc tcggcgagac
     1981 gctcaccgag acggagaagg cgttctcgct cggcttcgtg gcgcagaccg aggcgatcgt
     2041 gacggcggtg cgcatcgtct cgttcgtcgt gatcgtcatc atcttcgcgg tcgtcgccaa
     2101 caccatggcg atgacggcgc gcgagcgcct cgccgagtac gcgacgctga aagccctcgg
     2161 cttccacccg ggattcgtga tggcgctcat catgggcgag tcgatcatga tctccgcgct
     2221 gggcggggcg atcggcatcg cgctcacctt cccggtggcg gccggcttca agtcggcgat
     2281 gggatcgatg ttcccggtgt tctacgtgac gcaggagacg gtgctgatgc aggtggctgc
     2341 ggccgcgtcg gtcggaatcc tcgcggggat cctgccctcg attcgcgcgg cacgggtgaa
     2401 gatcgtggac gggctgaggt tcgcaggatg aagatccctt tctcgtacat tttccgcaac
     2461 ctctggaccc gcaagctcac gacgctgctc accgcgggcg acatggcgct ggtggtgttc
     2521 gtgttcgccg cggtgctgat gctcgacgcg ggattgaaga agacgctcgt gtccaccggc
     2581 agcctcgaca acgcgatcct gctgcggca
//
LOCUS       DASVII010000108         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_580881,
            whole genome shotgun sequence.
ACCESSION   DASVII010000108 DASVII010000000
VERSION     DASVII010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580881"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..333
                     /locus_tag="VF309_01435"
                     /pseudo
     CDS             37..333
                     /locus_tag="VF309_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
     gene            complement(654..>1509)
                     /locus_tag="VF309_01440"
     CDS             complement(654..>1509)
                     /locus_tag="VF309_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7465274.1"
                     /translation="TAALAFFGAAWLGERKLARSVEVRVVPVPYAKDAASLKLGKYLY
                     ESRGCGECHGADGRGIAFIDTPAGMYVKAPNITRGAGGVVSDYNEGDWVRAIRHGVNP
                     KGHALLVMPSEDYSRMNDADFAALVAYTRSLAPVAGENATIRLPSMVRALYGLGVILD
                     ASEKIDHRKPPSQPVPVAPTVEHGAYVANMCTGCHRASFAGGPIPGAPPEWPPAANLT
                     PGEGGAMARYESADKFVAMMRTGKRPDGGEVSKVMPFMSLRYLNDTDLTAIYAFLKAL
                     PPAKRAER"
BASE COUNT          229 a          514 c          527 g          239 t
ORIGIN      
        1 tgttgtccta gggtcgacga gcagagagga tgagccatgg cgagcaaggg gctgctggtc
       61 cggatggacg ccaagcacgg caagggcgtg gaaaacgagg tcttcctcgc ctcggcgctg
      121 ccgctggtgc gcgcggagcc cgccacgcag gcgtggttcg cgctgcgctt cgggcggggc
      181 gagtacggca tcttcgacgt gttcaccgac gacacgggac gcgactcgca cctgaacggc
      241 gacgtggcga aggcgctcat ggagaattcc gacgagctcc tcgacaaggc cccggagatc
      301 ggcaagctcg acgtgctcgc cgagaagatg ccgctcgccg attccaggga gccggtgacc
      361 aaggcgatcc tcctcacgtt caaggcgaag gccgggcacg aaggcgaggt cgagcagttc
      421 ctgcgcgacg cgcgcgcgct ggtgatggag gagccgaaga ccttcgcgtg gtgggcgatc
      481 cgccttgccg acggtcaata cggaatcttc gacgtctttc ccgacaatgg cgggcgcttc
      541 gcgcacctca ccggccacgt gccgcgcgag ctcgccaggc attcgctcac gctgctgggg
      601 agcgtgccgg acatgacgat gatcgacgtc gtgtcggcga agctctaggt tccctagcgc
      661 tcggcccgct tcgcgggagg cagcgccttc aggaacgcgt agatcgcggt gaggtcggtg
      721 tcgttcaggt acctgaggct catgaacggc atcaccttgc tcacctcccc gccgtcgggg
      781 cgcttgccgg tgcgcatcat cgcgacgaac ttgtcggcgc tttcgtagcg cgccatcgcg
      841 ccgccctcgc cgggcgtgag gttggcggcc ggcggccact cgggcggcgc tccgggaatc
      901 ggcccgcccg cgaagctcgc gcggtggcag cccgtgcaca tgttcgccac gtaggcgccg
      961 tgctccaccg tgggcgcgac cggcaccggc tgcgaagggg gcttgcgatg gtcgatcttc
     1021 tccgacgcgt cgagaatgac gcccagcccg tagagtgccc gcaccatcga cggcaggcgg
     1081 atcgtggcgt tctcgcccgc gaccggcgcc aggcttcgcg tgtatgcgac gagcgccgcg
     1141 aaatccgcgt cattcatgcg gctgtagtcc tcgctcggca tgaccaggag cgcgtggccc
     1201 ttcggattca cgccgtggcg gatcgcgcgc acccagtcgc cctcgttgta gtcggacacc
     1261 acgccgccgg ctccgcgcgt gatgttgggc gccttcacat acatgcccgc gggcgtgtcg
     1321 atgaaggcga tgccgcgccc gtcggccccg tggcactcgc cgcatccgcg cgactcgtag
     1381 aggtacttgc cgagcttcag cgacgccgcg tccttcgcat aaggcaccgg gaccacgcgc
     1441 acttccacgc tgcgcgcgag cttgcgctcg cccagccagg cggcgccgaa gaacgcaagc
     1501 gcggccgtc
//
LOCUS       DASVII010000109         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_113451,
            whole genome shotgun sequence.
ACCESSION   DASVII010000109 DASVII010000000
VERSION     DASVII010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113451"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1345
                     /locus_tag="VF309_01445"
     CDS             197..1345
                     /locus_tag="VF309_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX7465275.1"
                     /translation="MTEEFDALRDTVKRMCDAELAPRAAGIDAKNEFPQDMWKKLGEV
                     GLLGMTVPDEYGGTGLGYLAHVVAMEEVSRASASVGLSYGAHSNLCVNNLYLNGSEAQ
                     RRKYLPKLCSGEFVGALAMSEPGAGSDVVGSMACKAEKKGDRWIANGSKMWITNGPDA
                     HVAIVYMRTAPKDQGSKAMTAFLVEKGTKGFTTAQKLDKLGMRGSNTCELVFQDCEIP
                     QENVLGQVNGGSRVLMKGLDTERTVLSGGPLGIMQAAMDLVLPYVHERKQFDQPIGTF
                     ELMQGKLADMYTKLQASRAFSYRTAQALDAGRGRAARKDAAACLLFSSENAVQVALEA
                     IQAMGGNGYINDFPAGRLLRDAKLYDIGAGTNEIRRMLIGRELFEESV"
     gene            1359..>1963
                     /locus_tag="VF309_01450"
     CDS             1359..>1963
                     /locus_tag="VF309_01450"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7465276.1"
                     /translation="MAETIVIVGAKRTPIGSMQGKFNSVTASQLGSVAIKAAIEQAGV
                     KPNEVDDLIFGNVLGAGQGQAPARQAALGAGLDKSTPATTVNKMCGSGMKATMMAADE
                     LTSGQATIVVSGGMESMTNAPHLLPKSRAGFRYGSAQLLDHMAVDGLENAYDGKPMGY
                     FADKTAEKYGFTREQMDAYAKTSTERALSASKSGAFADEIAP"
BASE COUNT          377 a          655 c          646 g          285 t
ORIGIN      
        1 atcgagcggc tgcgccgcga gctcgaccag gcggtcctcg agatcgcgaa ggcccacgcg
       61 aaggtcgagg cataccggtc gatggtcccc gggacgattg cgcccgcgga ataggccgcg
      121 cccgcatttc gcccccagaa atgcgataat tgcactctcg ccacccgaaa agctgccttt
      181 gctattcgat acccacttga ccgaggaatt cgacgcgctg cgcgacacgg tgaagcgcat
      241 gtgcgacgcg gagctcgcgc cccgggccgc cgggatcgac gcgaagaacg agttcccgca
      301 ggacatgtgg aagaagctgg gcgaggtggg cctcctcggc atgacggtgc ccgatgagta
      361 cggcggaacg ggcctgggct acctggcgca cgtcgtggcg atggaggaag tctcgcgcgc
      421 ctcggcgtcg gtaggacttt cctacggcgc ccattcgaac ctgtgcgtca acaacctcta
      481 cctcaacggc tccgaggcgc agcggcgcaa gtacctgccg aagctctgct ccggtgaatt
      541 cgtcggcgcg ctcgcgatga gcgagcccgg cgccggctcc gacgtcgtgg gctccatggc
      601 gtgcaaggcc gagaagaaag gcgatcgctg gatcgccaac ggctcgaaga tgtggatcac
      661 caacgggccc gacgcgcacg ttgcaatcgt ctacatgcgc accgcgccga aggaccaggg
      721 ctcgaaggcg atgaccgcct tcctcgtcga gaagggcacg aaaggcttca ccaccgccca
      781 gaagctcgac aagctcggca tgcgcggctc caacacctgc gagctggtgt tccaggattg
      841 cgagatcccc caggagaacg tgctgggcca ggtgaacggc ggctcgcgcg tcctcatgaa
      901 ggggctcgat accgagcgca cggtcctttc cggcggcccg ctcggcatca tgcaggcggc
      961 gatggatctg gtcctgccct acgtgcacga gaggaagcag ttcgaccagc ccatcggaac
     1021 cttcgagctg atgcagggca agctcgccga catgtacacg aagctccagg cctcccgcgc
     1081 gttcagctat cgcaccgccc aggcgctcga cgccgggcgc gggcgcgccg cgcgcaagga
     1141 cgccgccgcg tgcctgctct tttcttcgga gaacgccgtt caagtggctc tcgaggcgat
     1201 ccaggcgatg ggcggcaacg gctacatcaa cgactttccc gccggacgat tgctacggga
     1261 cgccaagctc tacgacatcg gcgccggcac caacgagatc cgccgcatgt tgatcgggcg
     1321 cgagctattc gaagaatcag tttgaaccgg agaaacaaat ggcagaaacc atcgtcattg
     1381 tcggagcaaa gcgcaccccc atcggctcca tgcagggcaa gttcaactcg gtaaccgcct
     1441 cgcagctcgg gtcggtggcg atcaaggcgg ccatcgagca ggcgggcgtg aagcccaacg
     1501 aggtggacga tctcatcttc ggcaacgtgc tgggcgcggg ccagggacag gcgccggcgc
     1561 gccaggcggc gctcggcgcg ggcctcgaca agtcgactcc cgcgaccacc gtcaacaaga
     1621 tgtgcggctc cggcatgaag gccacgatga tggccgccga cgagctgacg tccggccagg
     1681 cgacgatcgt ggtgtccggc ggcatggaat ccatgaccaa cgcgccgcac ctgctgccca
     1741 agtcgcgcgc gggctttcgc tacggctcgg cgcagctcct cgaccacatg gcggtcgacg
     1801 gcctcgagaa cgcctacgac ggcaagccca tgggctactt cgcggacaag accgcggaga
     1861 agtacggctt cacgcgcgag cagatggatg cctacgcgaa gacctccacc gagcgcgcgc
     1921 tgagcgcgtc gaagtcgggc gccttcgccg acgagatcgc ccc
//
LOCUS       DASVII010000110         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_41657, whole
            genome shotgun sequence.
ACCESSION   DASVII010000110 DASVII010000000
VERSION     DASVII010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41657"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VF309_01455"
     CDS             <1..1137
                     /locus_tag="VF309_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEX7465277.1"
                     /translation="VLIPGLVNLHCHAAMTLMRGIADDVALMPWLQQHIWPAEAKHVS
                     DEFVHDGSLLAMAEMLRGGVTCVNDMYFFPEATARAALRAGIRAMLGVIAIEFPSAYA
                     ADAAGYLQKGLATRDAYQGETLLDFALAPHSPYTVGDEILKRIAVLAEELDLPIHCHV
                     HETLAEIEQGLAQHGVRPFERLRRMGLVGPRLIAVHCVHMDGIELDTMAREGVGVAHC
                     PSSNLKLASGIAPVAAMRERGIRVGVGTDGAASNNRLDVLAEMRMAALLAKVSSGDAG
                     VMGAADCLEMATLQGARALGLDDRIGSIASGKSADLAAVELSSVETLPCFDPVSHLVY
                     AAGREHVTHVWVEGRARLLDRALQGLDEEDLRDKARWWAKRLST"
     gene            1134..>1530
                     /gene="ubiG"
                     /locus_tag="VF309_01460"
     CDS             1134..>1530
                     /gene="ubiG"
                     /locus_tag="VF309_01460"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX7465278.1"
                     /translation="MTPEAKLNVDPSELAKFSALAHRWWDPTSEFRPLHEINPLRLAH
                     IEKLAGGLEGKRVIDVGCGGGILAEAMAARGAKVTGIDLADKPLKVAMLHRIESGSGV
                     DYRLTSAEAFAEESPGAFDVVTCMEMLEHV"
BASE COUNT          245 a          513 c          535 g          237 t
ORIGIN      
        1 gtgctcatcc cggggctcgt caacctgcat tgccatgccg cgatgacctt gatgcgcggc
       61 atcgcggacg acgtcgcgct gatgccgtgg ctgcagcagc acatctggcc cgccgaggcg
      121 aagcacgtga gcgacgagtt cgtgcacgac ggcagcctcc tcgcgatggc cgagatgctg
      181 cgcggcggcg tgacctgcgt gaacgacatg tatttctttc ccgaggccac cgcgcgcgcg
      241 gcattgcgcg cgggcatccg cgccatgctc ggcgtgatcg cgatcgagtt tccgagcgcc
      301 tatgccgccg atgcggcggg ctacctgcag aagggcctcg cgacgcggga tgcgtaccag
      361 ggggagaccc tcctcgactt cgccctggcg ccgcactcgc cctacacggt gggcgacgag
      421 atactcaagc gcatcgcggt gctcgccgag gagctcgacc tcccgatcca ctgccacgtg
      481 cacgagaccc tcgccgaaat cgagcagggg ctcgcgcagc acggcgtgcg gcctttcgag
      541 cgcctgcgcc gcatgggatt ggtcggcccg cgcctcatcg ccgtgcattg cgtgcatatg
      601 gatggcatcg agctcgacac gatggcgcgc gaaggcgtgg gcgtggccca ttgcccctcc
      661 tcgaatctca agctggcgag cggcatcgcg ccggtcgccg cgatgcgcga gcgcgggatt
      721 cgcgtcggcg tgggcaccga cggcgcggcg agcaacaatc gcctcgacgt gctggccgag
      781 atgcggatgg cggcgctcct cgcgaaagtg tcgagcggcg acgcgggcgt gatgggcgcg
      841 gccgattgcc tcgagatggc cacgctgcag ggcgcgcgag cgctcggcct tgacgatcgc
      901 atcggctcga tcgcctcggg caagagcgcg gacctcgcgg cggtggaact ttcgtcggtg
      961 gaaacgctcc catgtttcga tcccgtgtcg cacctcgtct acgccgcggg acgggagcac
     1021 gtgacccatg tgtgggtcga aggccgcgcg cggctcctcg accgcgcgct gcaagggctc
     1081 gacgaggaag atttgcgcga caaggcgcgt tggtgggcga agcgcctctc cacatgaccc
     1141 ccgaagccaa gctcaacgtc gatccatcgg agctcgcgaa attcagcgcg ctcgcccatc
     1201 gctggtggga ccccacgagc gagtttcgcc cgctgcacga gatcaatccg ctgcggctcg
     1261 cccacatcga gaagctcgcg ggcggcctcg agggcaagcg cgtcatcgac gtaggatgcg
     1321 gcggcggcat tctcgccgag gcgatggccg ccaggggcgc gaaagtgacc ggcatcgacc
     1381 tcgccgacaa gcccctcaag gtcgcgatgc tgcatcgcat cgaatcggga agcggcgtgg
     1441 actaccgatt gacctccgcg gaagcgttcg cggaggaatc gccgggcgca ttcgacgtgg
     1501 tcacttgcat ggagatgctc gagcacgtgc
//
LOCUS       DASVII010000111         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_581066,
            whole genome shotgun sequence.
ACCESSION   DASVII010000111 DASVII010000000
VERSION     DASVII010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581066"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VF309_01465"
     CDS             complement(<1..1099)
                     /locus_tag="VF309_01465"
                     /inference="COORDINATES: protein motif:HMM:NF006383.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HEX7465279.1"
                     /translation="MKILYTDVLVIGGGLAGLRLAIAARRRGHDALILSLVPAKRSHS
                     KAAQGGMQASLGNMTKSQGDNEDVHFEDTVRGSDWGADQRVVRMFVNTAPKAIRELAA
                     WGVPWSRVTRGDRDVVINSKKVTITERDEAHGLLAQRDFGGTKKWRACYVADGTGHAM
                     LTAVADRAIAESIPVHERVEALALVHDGKRCYGAVVRDLVTGELYACVAKATAIATGG
                     AGRLYRNTTNAVISEGIGNALVLETGCAALGNMEAVQFHPTAIFPAGILMTEGCRGDG
                     GLLKDVDGHRFMPDYEPEKRELASRDVVSRRMEEHIRKGKGVRSRFGEHLWLDITLLG
                     EAHIKRNLREVWEICHYFLGVDPVRELIPVRP"
     gene            complement(1096..>1581)
                     /locus_tag="VF309_01470"
     CDS             complement(1096..>1581)
                     /locus_tag="VF309_01470"
                     /inference="COORDINATES: protein motif:HMM:NF010072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HEX7465280.1"
                     /translation="FLAVRKFPVSYRQFTTFRGHMKMMRHEDTTLWAWQVGTGFALFF
                     LASVHLYVMLSRPDRIGPFESSDRVWSEHFWPLYVVLLLAVELHAGIGLYRLAVKWGW
                     LEGADPGTTRIRLKRLKWAITVFFLALGFATLAAYMKIGAGHQERYGERYVPAHLAAP
                     R"
BASE COUNT          242 a          529 c          568 g          242 t
ORIGIN      
        1 ccgggcgcac cgggatcagc tcgcgcaccg ggtccacgcc gaggaagtaa tggcagatct
       61 cccagacctc gcgcaggttg cgcttgatgt gggcttcgcc gaggagggtg atgtcgagcc
      121 agaggtgctc gccgaagcgc gagcgcacgc ccttgccctt gcggatgtgc tcctccatgc
      181 gccgcgagac cacgtcgcgc gaggcgagct cgcgcttctc cggctcgtag tcgggcatga
      241 agcgatggcc gtcaacgtcc ttcaacagcc cgccgtcgcc gcggcagccc tcggtcatga
      301 ggatgccggc ggggaagatc gcggtgggat ggaattgcac cgcctccatg ttgccgagcg
      361 cggcgcaccc cgtctccagg acgagcgcgt tgccgatgcc ctcggagatc acggcgttgg
      421 tcgtgttgcg gtagaggcgc cccgcgccgc cggtggcgat ggcggtcgcc ttcgcgacgc
      481 acgcgtagag ctcgccggtc acgaggtcgc gcaccaccgc gccgtagcag cgcttgccgt
      541 cgtggacgag ggcgagggcc tcgacgcgct cgtgcacggg aatcgactcg gcgatcgcgc
      601 gatcggccac cgcggtgagc atcgcgtgcc cggttccgtc ggcgacgtag caggcgcgcc
      661 acttcttggt gccgccgaag tcgcgctggg cgagcaggcc gtgcgcttcg tcgcgttcgg
      721 tgatggtgac cttcttgctg ttgatgacga cgtcgcggtc gcccctggtc acgcgactcc
      781 agggcacgcc ccacgcagcg agctcgcgaa tcgccttggg agccgtgttg acgaacatcc
      841 gcacgacgcg ctggtcggcg ccccagtcgc tgccgcgcac cgtgtcctcg aaatgcacgt
      901 cctcgttgtc gccctgcgac ttggtcatgt tgccgagcga ggcctgcatg ccgccctgcg
      961 cggccttcga atgcgacctc ttggccggca ccagcgacag gatcaacgcg tcgtggcccc
     1021 ggcggcgcgc ggcgatcgcg agccggaggc ccgcgaggcc accgccgatg acgagcacgt
     1081 cggtgtagag gatcttcacc gcggtgccgc gaggtgggcg ggcacgtagc gctcgccgta
     1141 gcgctcctgg tgcccggcgc cgatcttcat gtaggcggcg agcgtggcga agccgagcgc
     1201 gaggaagaag acggtgatcg cccatttgag gcgcttcagg cggatgcggg tggtgcccgg
     1261 gtcggcgccc tcgagccagc cccatttcac cgcgaggcgg tagaggccga tacccgcatg
     1321 cagctcgacc gcgaggagga gcacgacgta gagcggccag aaatgctcgc tccacacccg
     1381 gtcggacgat tcgaacgggc cgatgcgatc ggggcgcgag agcatcacgt agaggtgcac
     1441 cgaggcgagg aagaagagcg cgaagccggt gcccacctgc cacgcccaga gcgtcgtgtc
     1501 ctcgtgccgc atcatcttca tgtggccgcg gaacgtcgtg aactgccggt agctgaccgg
     1561 aaacttgcgc acggcgagga a
//
LOCUS       DASVII010000112         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_5946, whole
            genome shotgun sequence.
ACCESSION   DASVII010000112 DASVII010000000
VERSION     DASVII010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5946"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VF309_01475"
     CDS             complement(<1..725)
                     /locus_tag="VF309_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465281.1"
                     /translation="MNFRKLAVALALLGAAGAAIGQELRIALIVGKTGPFEAYAKETE
                     TGFMMGLEYLTGGKMELDGRKLKVIVKDDQLKPDLAKAALAEAYGDDKVAIAVGTTSS
                     GATLAMLPVAEEYKKVLIVEPAVADSITGEKWNRYIFRTSRNSTQDGLAGAATLMGEA
                     SIAFLAQDYAFGRDGVKAAKDALATMGSKAKVVHEEYAPQNTTDFTAPVQRIFDALKS
                     KPAPRILAIIWAGAHPLGKIADL"
     gene            complement(753..1481)
                     /locus_tag="VF309_01480"
     CDS             complement(753..1481)
                     /locus_tag="VF309_01480"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX7465282.1"
                     /translation="MTSLPNPRPMQRAKRTVAKRGRPVGGAKALEMREQLLDRAEALF
                     SHHGFHGVTIREVARAAKVDPALVHYYFETKQGLFDAVFVRRADVVNDERIRSLDAYA
                     LAAGEHITVEGCIEAFLEPIVRRSLEGDPGWKNYFRLVALVNSSAQWGGQMMARSFDP
                     VIRRLLELLRKCMPDAKEENLFWAYHFLSGALTLSLSETGRIDRLSGGLCSSSDTQAI
                     YRWMVPFVAAGFRRVCQPEGKPAA"
BASE COUNT          237 a          540 c          534 g          286 t
ORIGIN      
        1 ttcaggtcgg cgatcttgcc caacgggtgc gcgcccgccc agatgatggc gaggatccgc
       61 ggggccggct tcgatttcag cgcgtcgaag atgcgctgca cgggcgccgt gaagtccgtg
      121 gtgttctgcg gcgcgtattc ctcgtgcacc accttcgcct tgctgcccat ggtcgcgagc
      181 gcatccttcg cggccttcac gccgtcgcgg ccgaaggcgt agtcctgcgc gaggaacgcg
      241 atgctcgcct cgcccatgag ggtcgcggcg cccgccaggc cgtcctgcgt cgagttgcgg
      301 ctggtgcgga agatgtagcg attccatttc tctcccgtga tgctgtcggc caccgccggc
      361 tcgacgatca gcaccttctt gtactcctcg gccaccggca gcatcgcgag cgtcgctccc
      421 gaggatgtcg tgccgaccgc gatcgccacc ttgtcgtcgc cgtacgcctc cgcgagcgcg
      481 gccttggcga gatcgggctt gagctggtcg tccttcacga tgaccttgag cttgcgcccg
      541 tcgagctcca tcttcccgcc ggtcaggtac tcgaggccca tcatgaatcc ggtttcggtt
      601 tccttcgcgt aggcctcgaa gggcccggtc tttccgacga tgagggcgat gcgcaactcc
      661 tggccgatcg ccgcaccggc cgctcccagg agggcgagcg ccaccgcgag ttttcgaaag
      721 ttcaagttca tctccctgga atggatgcgc cgctatgcgg ccggctttcc ctcgggctgg
      781 caaacgcgcc tgaatcccgc ggcaacgaag ggcaccatcc accggtagat ggcctgcgta
      841 tcgctcgagg agcacagccc gccggagagc ctgtcgatgc ggccggtctc ggagaggcta
      901 agggtcaatg ccccggacag gaagtgatag gcccagaaga ggttctcctc cttcgcgtcc
      961 ggcatgcact tgcgcagcag ctcgagcagg cggcggatga ccgggtcgaa ggatcgcgcc
     1021 atcatctggc cgccccattg cgccgagctg ttgacgagcg cgaccagccg gaagtagttc
     1081 ttccatcccg gatcgccctc gagcgagcgg cgcacgatcg gctcgaggaa tgcctcgatg
     1141 cagccttcca ccgtgatgtg ttcgccggct gccagcgcat aggcgtccag ggaacggatc
     1201 cgctcgtcgt tcaccacgtc ggcgcgccgc acgaacacgg cgtcgaaaag cccctgcttg
     1261 gtctcgaaat agtagtgcac gagtgcgggg tcgaccttgg ccgcgcgcgc cacttcgcga
     1321 atggtgacgc cgtggaatcc atggtgcgag aacagcgcct cggcgcggtc gaggagctgc
     1381 tcgcgcattt cgagggcctt cgcgccgccg acggggcggc cgcgtttcgc gacagtgcgc
     1441 ttggcgcgct gcatcggcct ggggttcgga agcgacgtca cgctacggca cgctcgcgag
     1501 gaagctcgat agcgcggcct gcgcggcggg ctccatcagc atcggcgcgt ggcccactcc
     1561 gggcacttcg gcgtagcgca ggtccggagc ggccgtg
//
LOCUS       DASVII010000113         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_652919,
            whole genome shotgun sequence.
ACCESSION   DASVII010000113 DASVII010000000
VERSION     DASVII010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652919"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="rho"
                     /locus_tag="VF309_01485"
     CDS             <1..633
                     /gene="rho"
                     /locus_tag="VF309_01485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7465283.1"
                     /translation="DERPEEVTDMDRSVKGEVIASTFDEPATRHVQVAEMVIEKAKRL
                     VEHKKDVVILLDSITRLARAYNTVVPASGKVLTGGVDSNALQRPKRFFGAARNVEEGG
                     SLTIIATALIDTGSRMDDVIYEEFKGTGNMEIHLDRRMAEKRLYPSLSVNRSGTRREE
                     LLIAPEILPKVWILRKLLFGMDDLEATEFLLDKLRATKNNDEFFNSMKKG"
     gene            868..1134
                     /locus_tag="VF309_01490"
     CDS             868..1134
                     /locus_tag="VF309_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEX7465284.1"
                     /translation="MKDGIHPNYREVVFLDTSCDFGFVTRSTVATNETITWKDGKEYP
                     VYKIEISAESHPFYTGKQKIVDTAGRVDKFKKRYARKGTAKAAE"
     gene            1197..2855
                     /locus_tag="VF309_01495"
     CDS             1197..2855
                     /locus_tag="VF309_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7465285.1"
                     /translation="MAAIDAPLRGRIDYDGTRTPLKTVLFLVLCLAWLVPGLVGHDPW
                     KYDEAVVFGVVSEMLRSGDWVMFRIAGEPYFDKAPLFMWVAAAFGKVFGGILPLHDAT
                     RLATGFFMASTLALLSLACRELMGGRGLRMVVMLFIGCLGLLLRAHEMMPDLAGLAGV
                     ALGLYGVALASRRPRVGGLLLGLAIGVAFLGNGFLPASLLVALMVLLPAMSAFWRTRG
                     YLETVAIAVVSAAPLLAIWPLALASASPGYLQMWLAAATASRWGAPLTRDVGTDLLYF
                     VRIMPWYAWPAWPLAAWTLWRARRTLRDRRELMLPLAAFIAFFLVTSVFGDAREANGL
                     AFLLPLALLGVAELDTVPRGAASALDWFGMMTFLSFGALLWIGWFAAMTGSPEIAASW
                     IAKEVPGYKYRFSFIAFALAALLTLIWVVVVARSLRSTRRAVVNWAAGITMVWMMMMT
                     IGLPLVEEARSYRKVAARIVEELPPSFRCIARHNLGDPQRALLDYFANIRTVRDDLPQ
                     ASTCRALLVQAQPLRLPVIAPEWSETWRGSRSGDRNELFILYRR"
BASE COUNT          454 a          964 c          933 g          516 t
ORIGIN      
        1 gacgagcggc cggaggaagt gactgacatg gaccgcagcg tgaagggcga agtcatcgcc
       61 tccaccttcg acgagcccgc gacgcgccac gtgcaggtgg ccgagatggt gatcgagaag
      121 gccaagcgcc tggtggagca caagaaggac gtggtgatcc tgctcgactc gatcacccgc
      181 ctcgcccgcg cctacaacac tgtcgtgccc gcctccggca aggtgctcac gggcggcgtg
      241 gattccaacg cgctgcagcg cccgaagcgc ttcttcggcg cggcgcgcaa cgtcgaggag
      301 ggcggctccc tcaccatcat cgcgaccgcc ctcatcgaca ccggctcgcg catggacgac
      361 gtgatctacg aggaattcaa gggcaccggc aacatggaga tccacctgga tcgccgcatg
      421 gccgagaagc gcctctatcc gtcgctcagc gtgaaccgct ccgggacgcg ccgcgaggaa
      481 cttctgatcg ccccggagat ccttccgaag gtgtggatcc tgcgcaaact cctgtttggc
      541 atggacgacc tcgaggccac ggaattcctc ctggacaagc ttcgtgccac caagaacaac
      601 gacgagttct tcaactccat gaaaaaaggt taagcagagt ccctgccagg ctcgggaacg
      661 gcgttggagc ggacgaaggg cgtcgccgct caacgccagc ggatagaaaa gccggatgtg
      721 acgtttcgcg gcaaaaaacc ctgtccgctc aagggtctgg cactccacaa cgtacagtaa
      781 caaccccttg ctttcattca tgttttccgg gataatcact ggttcccggg ccgagttgga
      841 gttcgtgccc actacaaggc gaagatcatg aaagacggca tccaccccaa ttaccgcgaa
      901 gtcgtgttcc tcgacacgag ctgcgatttc ggtttcgtca cccgctcgac ggtcgcgacc
      961 aacgagacca tcacgtggaa ggacggcaag gagtatccgg tctacaagat cgagatctcc
     1021 gccgagtcgc atcccttcta cacgggcaag cagaagatcg tggacacggc cgggcgagtg
     1081 gacaagttca agaagcgcta cgcccgcaag ggcacggcga aggccgccga gtaatcggca
     1141 gcgctcgcgc tcgaaaaggc agcctcgcgg ctgccttttt tgtttaaatc cccaccatgg
     1201 ccgccataga cgcccccctg cgcggacgca tcgattacga cggcacgcgc acgccgctga
     1261 agaccgtgct gttcctcgtg ctgtgcctcg catggctcgt gccggggctc gtcggccacg
     1321 atccgtggaa gtacgacgag gcggtggtgt tcggcgtggt gagcgagatg ctgcgcagcg
     1381 gcgactgggt gatgtttcgc atcgccggcg aaccctactt cgacaaggcg ccgctcttca
     1441 tgtgggtcgc ggccgctttc ggcaaggttt tcggcggcat cctgccgctg cacgacgcca
     1501 cgcggctcgc cacggggttc ttcatggcgt ccacgctggc gctgctttcc ctggcgtgcc
     1561 gcgagctgat gggcgggcgc gggctgcgca tggtggtgat gctcttcatc ggctgcctgg
     1621 ggctcctcct gcgcgcccac gagatgatgc ccgacctcgc cggcctggcc ggcgtcgcgc
     1681 tcggtctcta tggcgtggcg ctcgcgtcgc gccggccgcg cgtgggcggc cttctcttgg
     1741 gcctggcgat cggcgtcgcg ttcctcggca acgggttcct gccggcgagc ctgctcgtgg
     1801 cgctcatggt gctgctgccg gcaatgagcg ccttctggcg aacccgcggc tacctcgaga
     1861 cggtcgccat cgcggtcgtc tccgccgcgc cgctcctcgc gatctggccg ctggccctcg
     1921 ccagcgcttc gccgggctac ctgcagatgt ggctcgcggc cgcgaccgca tcgcgatggg
     1981 gcgcgcccct gacccgcgac gtcggcacgg acctgctcta tttcgtgcgc atcatgccct
     2041 ggtacgcgtg gcccgcatgg ccgctcgccg cgtggacgct gtggcgcgcg cggcgcaccc
     2101 ttcgggaccg gcgcgagctc atgctgccgc tcgcggcgtt catcgcgttc ttcctggtca
     2161 cttcggtatt cggcgacgcg cgcgaagcga acggcctcgc gttcctgctt ccgctcgcgt
     2221 tgctcggggt ggccgagctc gacacggtgc cgcgcggcgc ggcgagcgcg ctcgactggt
     2281 tcgggatgat gaccttcctt tcgttcggcg cgctcctgtg gatcgggtgg ttcgcggcga
     2341 tgacgggaag cccggagatc gcggcttcat ggatcgcgaa ggaggtcccc ggctacaagt
     2401 accggttcag cttcatcgcc ttcgcgctcg cggcgctgct cacgctcatc tgggtcgtcg
     2461 tggtcgcgag gtcgcttcgc tcgacgcggc gcgcggtcgt caactgggcc gcgggcatca
     2521 ccatggtctg gatgatgatg atgacgatcg ggcttccgct ggtggaggaa gcgcgcagct
     2581 atcgcaaggt cgcggcgcgc atcgtcgaag agcttccgcc gagcttcagg tgcatcgctc
     2641 gccacaacct gggcgacccg cagcgggcgc tcctcgacta cttcgccaac atccgcacgg
     2701 tgcgcgacga tcttccccag gcctcgacat gccgcgcgct cctggtgcag gcccagcccc
     2761 tgaggcttcc ggtcatcgcc cccgagtggt cggaaacgtg gcgcggctcc cggtcgggcg
     2821 accgcaacga actgttcatc ctttatcgcc gctgaaaact ggtgtca
//
LOCUS       DASVII010000114         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_329558,
            whole genome shotgun sequence.
ACCESSION   DASVII010000114 DASVII010000000
VERSION     DASVII010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329558"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1569
                     /locus_tag="VF309_01500"
     CDS             <1..>1569
                     /locus_tag="VF309_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7465286.1"
                     /translation="ERTSSSRLGSANVTLQFELSRDINGAAREVQAAIQAARTDLPSS
                     LPNNPTYRKVNPADAPVMILALTSERFTPGQMYDIASTNIAQRISQMKGVGQVSVGGG
                     SLPAVRVELIPPLLNKLGIGFDEVRTAITSTNANRPKGFLDNGDTYWQVQANDQAKAA
                     AEYKPIVVAHRNGAAVRLGDLGEVLDSVQDTRNYGMANGKPAVLMIINRQPNANIIEV
                     VDSITALLPELRASIPASVNLDVMLERTSTIRASLHDVERTLMISVALVILVVFIFLR
                     NGRATLIPAVAVPISLIGTFAAMYVLGFSLNNLSLMALTIATGFVVDDAIVVLENVSR
                     HIEKGMRPFEAALLGAREVAFTVVSMSVSLVAVFIPILFMGGIVGRLFREFAVTLSVS
                     IGISLVVSLSTTPMMCSLLLRRADERKPGRIARFSEAAFDKIHKGYERSLGWALRLPL
                     LMLVVFFATMALTLWLYINIPKGFFPQQDTGRLIGSVRADQSISFQAMKEKLNHLAAI
                     VLADQAVESVTAFTG"
BASE COUNT          258 a          555 c          495 g          261 t
ORIGIN      
        1 cgagaggacg tccagctcgc ggctgggcag cgcgaacgtc acgctgcaat tcgagctctc
       61 gcgcgacatt aacggcgccg cgcgcgaggt ccaggcggcg atccaggcgg cgcgcaccga
      121 cctgccgtcg agccttccca acaacccgac ctaccgcaag gtgaacccgg ccgacgcgcc
      181 ggtcatgatc ctcgcgctca cctccgagcg cttcacgccg gggcagatgt acgacatcgc
      241 ctcgaccaac atcgcgcagc gcatctcgca gatgaagggc gtgggccagg tgagcgtcgg
      301 cgggggatcg cttccggcgg tgagggtcga gctcatcccg ccgctcctca acaagctggg
      361 catcggcttc gacgaggtgc gcaccgcgat caccagcacc aacgccaacc gccccaaggg
      421 ttttctcgac aacggcgaca cgtattggca ggtgcaggcc aacgaccagg ccaaggccgc
      481 cgcggagtac aagccgatcg tggtcgccca ccgcaacggc gccgcggtgc gcctgggcga
      541 cctgggcgaa gtcctcgaca gcgtgcagga cacgcgcaac tacggcatgg ccaacggcaa
      601 gcccgcggtg ctgatgatca tcaaccgcca gcccaacgcc aacatcatcg aggtggtgga
      661 cagcatcacc gcgctcctgc cggagctgcg tgcctcgatc ccggcgagcg tgaacctcga
      721 cgtgatgctc gagcgcacca gcaccatccg cgcctcgctc catgacgtcg agcgaacgct
      781 catgatctcg gtggcgctcg tgatcctcgt ggtgttcatc ttcctgcgca acggccgcgc
      841 gacgctgatc cccgcggtcg ccgtgcccat ctcgctcatc ggcacgttcg ccgcgatgta
      901 cgtcctgggc ttcagcctga acaacctgtc gctcatggcg ctcaccatcg cgacgggatt
      961 cgtcgtggac gacgcgatcg tcgtgctcga gaacgtctcg cgccacatcg agaagggaat
     1021 gcgcccgttc gaggcggcgc tcctcggagc gcgcgaggtg gcgttcaccg tcgtgtccat
     1081 gagcgtgtcc ctggtcgcgg tgttcatccc gatcctcttc atgggcggga tcgtcgggcg
     1141 cctcttccgc gagttcgcgg tgacgctgtc ggtgtcgatc ggcatctcgc tcgtcgtgtc
     1201 cctcagcacc acgccgatga tgtgctcgct cctgctgcgg cgggcggacg agcgcaagcc
     1261 cgggcgcatc gcgcgcttca gcgaggccgc gttcgacaag atccacaagg gctacgagcg
     1321 ctcgctcggc tgggcgctgc gcctgccgct gctgatgctc gtcgtgttct tcgccaccat
     1381 ggcgctgacc ctctggctct acatcaacat tcccaaggga ttctttccgc agcaggacac
     1441 cgggcgactc atcggctccg tgcgcgccga ccagtcgatc tccttccagg ccatgaagga
     1501 gaagctgaac catctcgcgg cgatcgtgtt ggccgaccag gcggtggaga gcgtcaccgc
     1561 tttcaccgg
//
LOCUS       DASVII010000115         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_113754,
            whole genome shotgun sequence.
ACCESSION   DASVII010000115 DASVII010000000
VERSION     DASVII010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113754"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1588
                     /locus_tag="VF309_01505"
     CDS             <1..>1588
                     /locus_tag="VF309_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX7465287.1"
                     /translation="GGGFEERYWNPLNTPILSAAGEVEYIVHRVEDATELVRLKRAEG
                     NLHATDILESIAEGFFSLDREWRFDYVNREGEIILGRGRAELIGRSIWDAYPGLIGSA
                     FEPIYRDAMDLRIPRMITDFFPDHDRWYEVHTYPARGGLCAYFRNVTEEKRGEAERGR
                     LVADSERQKRIYETALDNTPDLVYVFDRDHRFIYANHALLTMWGRTWEEAIGRNCLEL
                     GYEPWHAEMHDREIEQVVATRKPIRGEVPFVGTGGRRVYDYIFVPVVGPQGEVVAVAG
                     TTRDVTERNQNEQAILEQADRLADADRAKDEFLATLAHELRNPLAPLRNSLYLIRATG
                     GADAKAARVHEMMERQVNHLVRLVDDLLELSRISRGTFALRKERVDVAAIVRNAVETI
                     APLMESARHALTVTLPEEPLWLEGDPVRLAQILANLLDNAAKYTEDGGAIAVEAREDD
                     GFAAITVRDNGAGIAPEALPRMFEMFSRGERASVRGQGGLGIGLALARRLAQMHGGSL
                     EARSEGSGKGSEFTVRIPIAP"
BASE COUNT          267 a          565 c          547 g          209 t
ORIGIN      
        1 cgggaggcgg cttcgaggag cgctactgga acccgctcaa cacgccgatc ctttcggcgg
       61 cgggcgaagt cgaatacatc gtccaccgcg tggaggacgc gaccgagctc gtgcgcctga
      121 agcgcgccga gggcaacctg cacgccaccg acatcctcga gagcatcgcg gaaggcttct
      181 tcagcctcga ccgcgaatgg cgcttcgact acgtgaaccg cgaaggcgag atcatcctcg
      241 gcaggggccg cgcggagctg atcgggcgct ccatctggga cgcctatccg ggcttgatcg
      301 gcagtgcctt cgagccgatc taccgcgacg cgatggacct gcgcatcccg cgcatgatca
      361 ccgacttctt tcccgaccac gatcgctggt acgaggtcca cacctatccg gcgcgcggcg
      421 ggctgtgcgc gtacttccgc aacgtcaccg aggagaagcg cggcgaggcg gagcgcgggc
      481 gcctggtggc cgactccgag cggcagaagc gcatttacga gacggcgctc gacaacacgc
      541 ccgacctcgt ctacgtgttc gaccgcgatc accgcttcat ctacgccaac cacgccctcc
      601 tcacgatgtg ggggcgcacc tgggaagagg ccatcggcag gaactgcctg gagctgggct
      661 acgagccgtg gcacgccgag atgcacgacc gcgagatcga gcaggtggtg gccacccgca
      721 agccgattcg cggcgaggtg cccttcgtcg gcaccggcgg gcggcgcgtc tacgactaca
      781 tcttcgtccc ggtggtgggt ccgcaaggcg aggtcgtcgc ggtggcgggc accacgcgcg
      841 acgtgaccga gcgcaatcag aacgagcagg cgatcctcga gcaggccgac cgcctcgccg
      901 acgccgaccg cgcgaaagac gagttcctcg ccaccctcgc gcacgagctg aggaacccgc
      961 tcgcgccgct gcgcaactcg ctgtacctga ttcgcgccac gggcggcgcc gacgcgaagg
     1021 ccgcgcgcgt ccacgagatg atggagcggc aggtcaacca cctggtgcgc ctggtcgacg
     1081 accttctcga gctctcgcgc atcagccgcg gcacgttcgc gctgcgcaag gagcgcgtcg
     1141 acgtggccgc gatcgtgcgc aacgccgtcg agaccatcgc gccgctgatg gagtccgcgc
     1201 gccacgcgct caccgtgaca cttccggaag agccgctctg gctggaaggc gacccggtgc
     1261 gcctcgccca gatcctcgcc aacctcctcg acaacgcggc caagtacacc gaggacggcg
     1321 gcgcgatcgc ggtcgaggcg cgcgaggacg acggcttcgc cgcgatcacg gtgcgcgaca
     1381 acggcgccgg catcgccccc gaggcgctgc cgcgcatgtt cgagatgttc agccgcggcg
     1441 agcgcgccag cgtgcgcggg cagggaggcc tgggcatcgg cctcgcgctc gcgcggcgcc
     1501 tggcgcagat gcacggcggc agcctcgagg cgaggagcga aggctccggc aaggggagcg
     1561 agttcaccgt gcgcatcccg atcgcccc
//
LOCUS       DASVII010000116         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_329584,
            whole genome shotgun sequence.
ACCESSION   DASVII010000116 DASVII010000000
VERSION     DASVII010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329584"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VF309_01510"
     CDS             <1..1989
                     /locus_tag="VF309_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7465288.1"
                     /translation="GLEEETMARKDADPKVDRRKFLAGFAAAGVAGAVTPPAARGADA
                     VAQDTPRAPSALAPSVKTVEAETRTPRELARVPGIPGSDFMVDVIKTLDIKYLPANCA
                     SSYRALHESLIDYGANRMPEFLTCMHEESAVAMAHGYFKVAGKPLLTLCHGTVGLQHA
                     AMGIYNAWCDRVPMIVIGGNELDAAHRPPGVPTIHSAQDINALVRDFTKWDDTPVSLQ
                     HFAQSFVRAYKIAMTPPYGPVMISLDAGLQQEPIRDHGEKPYIPRYVPTSPPQGDSGA
                     VKEAARLLAGAENPVIVADRAARTPNGVKLLVELAELLQARVVDQGGRMNFPTTHYLT
                     APPGAVGNADVILGLELSDFWGTVNAFTDNGENGGIGRNSARMKGDAKLIGISSVDLN
                     TKSNYQDFQRFQVIDVQMAADAEATLPALIEAVRAAIPNERKAAIAARAEPARKAYAE
                     MRDRARQAAAVGWDANPISTARMVMETFAAVKDLDWSLVSAESPRFDWPQRLWPLEKH
                     HHWLGRSGGYGVGYAAPASVGAALANRDLGRFSVCFQPDGDLMYAPGVLWTAARHKIP
                     LLSVMHNNRGYHQEVMHVQRMANFRNRAANLGPDMGPVGTSIENPDIDYRKLAESMGW
                     WAKGPIKDPSQLGPALREAVAVVRSGQPALLDVWTQPR"
BASE COUNT          338 a          695 c          689 g          282 t
ORIGIN      
        1 gggctggagg aggagaccat ggcacgcaag gacgcagatc cgaaggtcga tcgccgcaag
       61 ttcctcgccg ggttcgccgc ggcgggcgtc gcaggggcgg tgacgccgcc cgcggcgcga
      121 ggcgccgatg ccgtggcgca ggacacgccg cgcgcgccgt ccgcgctggc gccctcggtg
      181 aagaccgtcg aggccgaaac aaggacgccg cgggagctcg cgcgcgtccc gggcattccg
      241 ggttccgatt tcatggtcga cgtcatcaag acgctcgaca tcaagtacct gccggcgaac
      301 tgcgcgtcca gctatcgcgc cctccacgag tcgctcatcg actatggcgc gaaccggatg
      361 ccggaattcc tcacctgcat gcacgaggaa tccgcggtcg ccatggcgca cggatacttc
      421 aaggtcgcgg gaaagccgct cctcacgctc tgccacggca ccgtcggcct gcagcacgcc
      481 gcgatgggca tctacaacgc gtggtgcgac cgcgttccga tgatcgtgat cggcggcaac
      541 gagctcgacg ccgcgcatcg cccgccgggc gtgccgacca tccactccgc gcaggacatc
      601 aacgcgctgg tgcgcgactt caccaagtgg gacgacacgc cggtgtcgct gcagcatttc
      661 gcgcagtcct tcgtgcgcgc gtacaagatc gcgatgacgc cgccttacgg gccggtgatg
      721 atctcgctcg acgccggatt gcagcaggag ccgatcaggg accacggcga gaagccctac
      781 atcccgcgct atgtgccgac ctcgccgccg cagggcgatt cgggcgcggt gaaggaggcg
      841 gcgcggctgc tcgcgggcgc cgagaacccg gtgatcgtgg ccgaccgcgc ggcgcgcacg
      901 cccaacggcg tgaaactgct ggtcgagctc gccgagctcc tgcaggcgcg cgtggtcgac
      961 cagggcgggc gcatgaactt cccgacgacg cactacctca cggcgccgcc gggcgcggtc
     1021 ggcaatgccg acgtgatcct cggcctcgag ctctcggact tctgggggac ggtgaacgcc
     1081 ttcaccgaca acggcgagaa cggcggcatc ggcaggaatt cggcgcggat gaagggcgac
     1141 gcgaagctca tcggcatcag ctccgtcgac ctcaacacca agtcgaacta ccaggacttc
     1201 cagcgcttcc aggtgatcga cgtgcagatg gccgccgatg ccgaagcgac gctgcccgcc
     1261 ctcattgaag cggtgagagc cgcgattccc aacgagcgca aggcggcgat cgcggcgcgc
     1321 gccgagccgg cgaggaaggc gtatgccgag atgcgcgatc gcgcgcgcca ggcggcggcg
     1381 gtcgggtggg atgcgaatcc catcagcacg gcgcgcatgg tgatggagac tttcgcggcg
     1441 gtgaaggatc tcgactggtc gctcgtctcc gccgaaagcc cgcgcttcga ctggccgcag
     1501 cggctctggc ccctcgagaa gcaccaccat tggctcggcc ggtcgggcgg ctacggcgtg
     1561 ggctacgccg cgcccgcttc ggtcggcgcg gcgctcgcca accgcgacct cggccgcttc
     1621 tcggtctgct tccagcccga cggcgacctc atgtacgcgc ccggcgtgct gtggaccgcc
     1681 gcgcggcaca agatcccgct cctctcggtg atgcacaaca atcgcggcta tcaccaggaa
     1741 gtgatgcacg tgcagcgcat ggcgaatttc cgcaatcgcg ccgccaacct ggggcccgac
     1801 atggggccgg tcggcaccag catcgagaac cccgacatcg actatcgcaa gctcgccgaa
     1861 tccatggggt ggtgggcgaa gggcccgatc aaggacccgt cgcaattggg ccccgcgctg
     1921 cgtgaagccg tcgccgtggt gagatcgggc cagcccgcgc tcctcgacgt gtggacgcaa
     1981 cctcgctgag gagatcgacg atgc
//
LOCUS       DASVII010000117         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_473324,
            whole genome shotgun sequence.
ACCESSION   DASVII010000117 DASVII010000000
VERSION     DASVII010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473324"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VF309_01515"
     CDS             <1..695
                     /locus_tag="VF309_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX7465289.1"
                     /translation="IFSFYYRFMLSPAVLATAMRGAFNMHGSLLPKFRGRAPVNWAVL
                     RGETSTGATLHEMVEKPDAGRIVDQFPVPILPDDRAIDVFRKVTVAAELLLDRALPGV
                     LDGMAPLIAQDLAQGGYYRGRKSDDGRIDWAKGAKEIHDLVRAVAPPYPGAFTMLGAQ
                     KLRLDATRLMPGTVPAAPATLRFDGTHLVARCADGRSLRILECDLDGVALQAAAFEEL
                     FGREPVPLGEIP"
     gene            692..>1570
                     /locus_tag="VF309_01520"
     CDS             692..>1570
                     /locus_tag="VF309_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX7465290.1"
                     /translation="MKKLCILGVNGFIGHHLSNRILAETDWEVYGMDMGSERIADLMR
                     NPRFHFFEGDITINKEWIEYHVRKCDVILPLVAIATPATYVREPLKVFELDFEANLPI
                     VRSCVKYGKRVLFPSTSEVYGMCEDAEFDPYQSNLVLGPIDKQRWIYSCSKQLMDRVI
                     FAYGSMGQLDFTLFRPFNWIGAGLDSIHTAKEGSSRVITQFLGHIVRGEPIKLVDGGT
                     QKRAFTYIDDGIAALMKIIENKGGIATGKIYNVGNPKNNYSVRSLAQMMLDLAKDYAE
                     YRDNAAKVKLVEVTSGD"
BASE COUNT          278 a          532 c          491 g          269 t
ORIGIN      
        1 tcatcttctc gttctactac cggttcatgc tctcgccggc ggtgctcgcg acggcgatgc
       61 gcggcgcgtt caacatgcac gggtccctcc tgccgaagtt tcgcggccgc gctccggtca
      121 actgggcggt gctgcgcggc gaaacctcga ccggtgcgac gctgcacgag atggtcgaga
      181 agcccgatgc ggggcgaatc gtcgatcagt tcccggtgcc gatccttccc gacgaccgtg
      241 ccatcgacgt ctttcgcaag gtgaccgtgg ccgcggagct gctcctcgat cgcgcgctgc
      301 ccggcgtcct cgacggcatg gcgccgctca tcgcccagga cctcgcgcag ggcgggtatt
      361 accggggacg caagtccgac gacgggcgca tcgactgggc caagggcgcg aaggagatcc
      421 acgacctggt tcgcgccgtg gcgccgccct accccggcgc cttcacgatg ctcggcgccc
      481 agaagctgcg cctcgatgcc acgcgcctca tgccgggaac ggtgccggcc gcgccggcga
      541 ccctgcgctt cgacggcacg cacctggtgg cccgctgcgc ggacggccgc tccctcagga
      601 tcctcgagtg cgacctggac ggcgtcgcgc ttcaagcggc ggcattcgag gagttgttcg
      661 gccgcgagcc ggttccgctt ggagaaatcc cttgaaaaaa ctctgcatcc tcggcgtcaa
      721 cggcttcatc gggcaccatc tttccaaccg catcctcgcc gagacggact gggaggtgta
      781 tggaatggac atgggctcgg agcgcatcgc cgacctcatg cgcaacccgc gcttccattt
      841 cttcgaaggc gacatcacga tcaacaagga gtggatcgaa taccacgtgc gcaagtgcga
      901 cgtgatcctg ccgctggtgg cgatcgcgac gcccgcgacc tacgtgcgcg agccgctcaa
      961 ggtcttcgag ctcgacttcg aggcgaacct gccgatcgtg aggagctgcg tgaagtacgg
     1021 caagcgcgtg ctctttccct cgacctccga ggtctacggc atgtgcgagg acgccgaatt
     1081 cgatccgtac cagtcgaacc tcgtgctcgg ccccatcgac aagcagcgct ggatctactc
     1141 gtgctcgaag cagctcatgg atcgcgtgat cttcgcttac ggttccatgg ggcagctcga
     1201 cttcacgctc ttccggccgt tcaactggat cggcgccggc ctcgattcca tccatacggc
     1261 gaaggaaggc agctcccggg tcatcacgca gttcctcggg cacatcgtcc gcggcgagcc
     1321 catcaagctc gtcgacggcg gcacgcagaa gcgcgcgttc acctacatcg acgacggcat
     1381 cgcggcgctc atgaagatca tcgagaacaa gggcgggatc gcgaccggca agatctacaa
     1441 tgtcggcaac ccgaagaaca actactcggt gcgcagcctc gcgcaaatga tgctcgatct
     1501 cgcgaaggac tatgccgaat accgcgacaa cgccgcgaag gtgaagctgg tcgaggtcac
     1561 ctcgggcgac
//
LOCUS       DASVII010000118         3762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_6054, whole
            genome shotgun sequence.
ACCESSION   DASVII010000118 DASVII010000000
VERSION     DASVII010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6054"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="VF309_01525"
     CDS             <1..1595
                     /locus_tag="VF309_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX7465291.1"
                     /translation="AELKPFADYKQRVDPHGRFNRGKLLAGGDLRHAYTPSFALIGHE
                     SLILEQTDVGAIADSVKDCLRCGKCKPVCATHVPRANLLYSPRNKILALGLLTEAFLY
                     EERTRRGVSIRHFDEYGDVADHCTVCHKCESPCPVDIDFGDVSIAMRNLLAKLRKRRF
                     NIGTWAAMQFLNAQDPAKIKVIKALMIDFGFRAQRFANKVWGRTPFVQRVVRHPPASV
                     GKAPLASQVIHFINKPMPGGLPKKTARALLDVEDPAMVPIIRDPAKMNEDSEAVFYFP
                     GCGAERLFGQVGLATQALLYHVGSQCVLPPGYLCCGYPQTAAGRADKGEQITIQNRVL
                     FHRVATTLNYLDIKTVVVSCGTCMDQLQKYEFDKIFPGCRLLDIHEYLMEKNVRVEGV
                     KGVRYMYHDPCHSPMKTYQPLKVVNELMGQEVGLNDRCCGESGTFAATRPDIATQVRH
                     RKEEEMRKGAAKVRADGFSGEVKVLTSCPSCLQGLQRYNPDSGTSADYIVVEMARSLL
                     GEDWMKEFVERANRGGIERVLL"
     gene            1558..2133
                     /locus_tag="VF309_01530"
     CDS             1558..2133
                     /locus_tag="VF309_01530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX7465292.1"
                     /translation="MPTAGASSAFCCDRPAASAAPRIRLPFQAVRLKMLASCAGFDNH
                     RSQRRMNSSSAPCEFCAAPGGAVLWQNDLCRVVRADEPDYPGFCRVILKRHATEMADL
                     GEDERNGLMAVVFAVEAVVRETMQPHKMNLASLGNMIPHVHWHVVPRFEDDRHYPGPI
                     WAAPRRERGVAPERAKRAEGIAAALARRLGS"
     gene            2136..3740
                     /locus_tag="VF309_01535"
     CDS             2136..3740
                     /locus_tag="VF309_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465293.1"
                     /translation="MASLTPPAPRALAYSDGRGCKEWLNALPLTNIPQAQSLVLESLS
                     ALNAAEVDDLERLKCLELMRDKIAFLQGEQRSRYFGKSLPLSVNDSNAWATGRTLLEE
                     METGYRHCLAAAEAGGGLAGHKALIAQRMARYIGTQMMFHAIVYRRFDPQLWTRLHRA
                     YLDADHGGYAAERVKDSLEGDGGLSSVAETYVQIVLLQAAFLSEMTAPQMDFAEALTR
                     LWARKVALTDQSPETNRGRLDPLVVDLAKPIGARPQPRGELLTAQRALDIHALSISMR
                     KRIHGLQNGEDLATLGLPAEAGALDVLMELQRLHKLWCEGAPPRPAAQPASKPTAGLV
                     FGMAEIHFFVGAGKPFEQPDKKREMTRQEKQDIEVFGRVREQTQSKMVADFNYTVEAW
                     ALVDEMLGAVRVQRPATASKGIAIGRLVAIRMGDAGPFYLGALSELASETDGRIIATV
                     TLFPGKPETVIVRAGDARNRANAHWTQGLSLPALEKLNIPASLVVPSGMGTRGRGIEM
                     WREGVRESTVEEVLQRGVDFDRVTTF"
BASE COUNT          623 a         1260 c         1293 g          586 t
ORIGIN      
        1 ccgcggagct gaagcccttc gccgattaca agcagcgcgt ggatccccat gggcggttta
       61 atcgcggcaa gctcctcgcg ggcggcgacc tgcggcacgc ctacacgccg tcctttgcgc
      121 tgatcggcca cgagtcgctc atcctcgagc agaccgacgt cggcgcgatc gccgactcgg
      181 tgaaggattg cctgcgctgc ggcaagtgca agcccgtgtg cgcgacgcac gtgccgcgcg
      241 ccaacctgct ctacagcccg cgcaacaaga tcctcgcgct cggccttctc accgaggcgt
      301 tcctctacga ggagcggacg cgccgcggcg tgtccatccg gcacttcgac gagtacggcg
      361 acgtcgccga ccattgcacg gtttgccaca agtgcgagtc gccgtgcccg gtggacatcg
      421 acttcggcga cgtgtcgatc gcgatgagga acctcctcgc gaagcttcgc aagcggcggt
      481 tcaatatcgg cacctgggcc gcgatgcagt tcctcaacgc gcaggacccc gcgaagatca
      541 aggtgatcaa ggcgctgatg atcgacttcg gctttcgcgc gcagcgcttc gcgaacaagg
      601 tgtggggccg cacgccgttc gtgcagcgcg tggtgaggca tcccccggcg agcgtcggca
      661 aggcgccgct ggcctcgcag gtgatccatt tcatcaacaa gcccatgccc ggcgggctgc
      721 cgaagaagac ggcgcgcgcc ctcctcgacg tcgaggatcc ggcgatggtg ccgatcatcc
      781 gcgatcccgc gaagatgaac gaggactccg aggcggtgtt ctattttccc ggctgcgggg
      841 cggagcggct cttcggccag gtggggctcg ccacccaggc gctcctctat cacgtcggaa
      901 gccagtgcgt gctgcctccc gggtacctct gctgcggcta cccgcagacc gccgcgggcc
      961 gcgccgacaa gggcgagcag atcacgatcc agaaccgcgt gctcttccat cgcgtggcca
     1021 cgacgctcaa ctacctcgac atcaagaccg tggtggtttc ctgcggcacc tgcatggacc
     1081 agctgcagaa atacgagttc gacaagatct tcccgggctg ccgcctcctc gacatccacg
     1141 agtacctgat ggagaagaac gtgcgggtcg agggcgtgaa gggcgtgcgc tacatgtacc
     1201 acgacccgtg ccactcgccg atgaagacct accagcccct gaaggtcgtg aacgagctca
     1261 tggggcagga ggtgggcctc aacgatcgct gctgcggcga gtcggggacc ttcgccgcga
     1321 cccggcccga catcgcgacc caggtgcgcc accgcaagga agaggagatg cgcaagggag
     1381 cggcgaaggt gcgcgccgac ggcttcagcg gcgaggtgaa ggtcctcacc tcgtgccctt
     1441 cgtgcctcca gggattgcag cgatacaatc cggattcggg aacctcggcg gactacatcg
     1501 tcgtcgagat ggcgagaagc ctgctcggcg aggactggat gaaggagttc gtcgagcgtg
     1561 ccaaccgcgg gggcatcgag cgcgttctgc tgtgaccggc cggcggcttc ggccgcgccg
     1621 cgcattcgtc tgccatttca ggccgttcgg ctgaaaatgc tggccagctg cgcgggtttc
     1681 gacaaccatc ggagtcaacg tcgcatgaat tcgtcttccg caccttgcga gttctgcgcg
     1741 gccccgggcg gggccgtgct ctggcagaat gatctttgcc gcgtggtgcg ggcggacgag
     1801 ccggactatc cgggtttttg ccgggtgatc ctcaagcgcc atgcgaccga gatggccgac
     1861 ctcggggagg acgaacgcaa tggcctcatg gcggtggtat tcgcggtgga ggccgtggtg
     1921 cgcgagacga tgcagcctca caagatgaac ctggcgagcc tgggcaacat gattcctcac
     1981 gtgcattggc acgtcgtgcc tcgtttcgag gacgatcgcc actatcccgg cccgatctgg
     2041 gccgctccgc ggcgcgagag aggcgtcgcg ccggagcggg ccaagcgcgc cgaagggatc
     2101 gccgccgccc tggcccggcg gctcgggagc tagcgatggc ctccctcacc ccgcccgccc
     2161 cgcgcgccct cgcctatagc gacgggcggg gctgcaagga gtggctgaac gcgctcccgc
     2221 tcaccaacat cccgcaggcg caatcgctgg tgctcgaatc gctgagcgcg ctcaacgccg
     2281 ccgaggtgga cgatctcgag cgcctcaagt gcctcgagct catgcgcgac aagatcgcct
     2341 tcctgcaggg ggagcagcgc tcgcgctact tcggcaagag cttgccgctg tcggtgaacg
     2401 acagcaatgc gtgggccacg ggccgcacgc tcctcgagga gatggagacc ggataccgcc
     2461 attgcctcgc ggccgccgag gcgggcggcg ggctcgccgg ccacaaggcg ctcatcgcgc
     2521 agcgcatggc gcgctatatc ggcacgcaga tgatgttcca cgcgatcgtg taccggcgct
     2581 tcgacccgca gctctggacg cggctgcacc gggcgtacct cgacgccgac catggcggat
     2641 acgccgcgga acgcgtgaag gactcgctcg agggcgacgg ggggctttcg agcgtcgccg
     2701 aaacgtacgt gcagatcgtg ttgctccagg ccgcgttcct ttccgagatg accgcgccgc
     2761 agatggattt cgccgaggcg ctgaccaggc tctgggcgcg aaaggtcgcg ttgacggacc
     2821 agtcgcccga gacgaaccgg ggcaggctcg atccgctcgt ggtcgacctg gcgaagccga
     2881 tcggcgctcg gccgcagccg cgcggcgagc tcctcacggc gcagcgcgcg ctcgacatcc
     2941 acgccctttc gatcagcatg cgcaagcgca tccacgggct gcagaacggc gaggacctcg
     3001 cgaccctcgg gctgcccgcg gaggcgggcg cgctcgatgt gctcatggag ctgcagcgct
     3061 tgcacaagct gtggtgcgag ggggcgcccc cgcgtcccgc ggcgcagccc gcgtcgaagc
     3121 cgaccgccgg cctcgtcttc gggatggccg agatccactt cttcgtgggt gccgggaagc
     3181 ccttcgagca gcccgacaag aagcgcgaga tgacgcgcca ggagaagcag gacatcgagg
     3241 tcttcggccg cgtgcgcgag cagacccaga gcaagatggt cgcggacttc aactacacgg
     3301 tcgaggcatg ggcgctcgtc gacgagatgc tgggcgcagt tcgcgtgcag cgcccggcga
     3361 ccgcgtcgaa ggggatcgcg atcgggcgcc tggtcgccat tcgcatgggc gatgcggggc
     3421 ccttctacct gggcgcgctg agcgagctcg ccagcgagac cgacgggcgc atcatcgcca
     3481 cggtcacgct ctttccgggc aagcccgaga ctgtcatcgt gcgcgcgggc gacgcgcgca
     3541 atcgcgccaa cgcgcactgg acgcaggggc tttcgcttcc cgcgctcgag aagctcaaca
     3601 ttccggcctc cctcgtggtt cccagcggca tggggacgcg gggccgcggt atcgagatgt
     3661 ggcgcgaggg cgtccgggaa tcgacggtgg aggaagtgct ccagcgcggc gtcgacttcg
     3721 accgcgtcac gactttctag ggccgacccg cgagtacttg gg
//
LOCUS       DASVII010000119         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_257602,
            whole genome shotgun sequence.
ACCESSION   DASVII010000119 DASVII010000000
VERSION     DASVII010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257602"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VF309_01540"
     CDS             complement(<1..1251)
                     /locus_tag="VF309_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7465294.1"
                     /translation="MAEVELKLDVDRGAHAALLRAAALAGTKPRRMRMTSLYFDTPDC
                     ALAGAGMALRLRRSGGRWAQCLKAGRSGTGGLHARDEWEFERPGPVLDLSLFAETPLA
                     ALDGAASLHRTLAKAFTVEFQRTAWQLEPAAGSRLEVALDCGFVESRGRREEISEVEI
                     ECVAGDPAHAFDLARRLLDEVALRPSATAKAARGYRLFNRMPLRPVKATPARLAASMG
                     PLPAARALVAAALDHLQANEKGVPRAAAPEFVHQARVALRRMRSALRMFDAAIGDERA
                     KAWRAEWSEATSALGAARDWDVFATESLPALAAAYGDTSTSRTLMRQAASRRRQCRED
                     ARAALRSPRYARAILDLASWLAGIGEEAAESATEPLADFASRWIRKRHRSLLAELSGL
                     AGMPAEDRHRVRIDAKRLRYGVEGL"
     gene            complement(1254..2012)
                     /locus_tag="VF309_01545"
     CDS             complement(1254..2012)
                     /locus_tag="VF309_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX7465295.1"
                     /translation="MAQDKAPSAADGARLDFSEAMSYGDYLHLDRMLAAQQPRSRDHN
                     EMLFIIQRAAFKMLARVARVMAQLNQSWDVLATLTPAEYSSFRNDLGAASGFQSFQYR
                     AIEFLLGNKDAVRMEPHRHRAEIFAELDALHREPSLYDEAIRLLGRRGFRIDGECVER
                     DWRVAYAFNESVCEAWVAVYRSTAENWDLYELAEKLVDLEDAFRQWRFRHATTVERVI
                     GFKRGTGGTSGVNYLRRVVEVELFPELWRARTAL"
     gene            2090..>2604
                     /locus_tag="VF309_01550"
     CDS             2090..>2604
                     /locus_tag="VF309_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7465296.1"
                     /translation="MHATLPLLVETDFPPLGRRLLDTLQVNVGYVCNQTCLHCHVNAG
                     PTRTESMTRETADQVLEYLAASGATTLDLTGGAPELNAHFRHLVTGARSLGARVIDRC
                     NLTILFEPGQEDLARFLALNRVEIVASLPCYTAELVDRQRGDGVYERSIAAIRILNDL
                     GYGRDGSALTL"
BASE COUNT          384 a          907 c          917 g          396 t
ORIGIN      
        1 gaggccttcc acgccatagc gcaggcgctt cgcgtcgatg cgcacgcgat ggcgatcttc
       61 ggccggcatg cccgcgaggc ccgagagctc cgcgaggagg ctcctgtgcc gcttgcggat
      121 ccagcgcgac gcgaagtcgg ctagcggctc ggtggccgat tcggcggcct cttccccgat
      181 gcccgcaagc cagctcgcca gatcgaggat cgcgcgcgca tagcgaggag agcgcagcgc
      241 cgcccgcgcg tcctcgcggc attgccgccg gcgcgacgcc gcctgccgca tgagcgtgcg
      301 tgatgtcgat gtatcgccgt aggccgcggc gagcgcggga agcgattcgg tcgcgaagac
      361 gtcccagtcg cgcgccgcgc cgagcgcgct cgtggcctcg ctccactcgg cgcgccacgc
      421 cttcgcgcgc tcgtcgccga tcgcggcgtc gaacatgcgt agtgccgagc gcatccggcg
      481 caatgccacg cgcgcctggt gcacgaattc cggcgccgcc gcgcgcggca cgcctttctc
      541 gtttgcctgc aggtggtcca gcgccgcggc gacgagcgcc cgcgccgcgg ggagcgggcc
      601 catgcttgcg gcgaggcggg ccggcgtagc cttcaccgga cgaaggggca tgcggttgaa
      661 gagccgatag ccgcgcgccg ccttggcagt cgcgctgggg cgaagcgcca cttcgtcgag
      721 aaggcgccgg gcgaggtcga aagcgtgggc gggatcgccc gcgacgcact cgatctccac
      781 ctccgagatc tcttcgcgcc gcccgcggct ttcgacgaag ccgcagtcga gcgcgacctc
      841 gaggcggctg ccggcggcgg gctcgagctg ccaggcggtg cgctggaact cgacggtgaa
      901 cgctttcgcg agagttcggt gcaggctcgc ggcgccgtcg agcgcggcca gcggtgtttc
      961 ggcgaagagg gaaaggtcga gcacggggcc gggccgttcg aattcccatt cgtcgcgcgc
     1021 gtggaggcct cccgtgccgc tgcggccggc cttgaggcac tgggcccagc gcccgcccga
     1081 gcgccgcagg cgcagcgcca tcccggcgcc ggcgagcgcg caatcgggcg tgtcgaagta
     1141 gaggctcgtc atgcgcatgc gccggggctt tgtgccggcg agggccgccg cgcgcaggag
     1201 cgccgcgtgc gcgccgcgat ccacatccag cttgagctcg acctcggcca tgcctagagc
     1261 gcggttcgcg cgcgccacag ctcgggaaag agctcgactt cgacgacgcg gcgcaggtag
     1321 ttcactcccg acgtgcctcc cgtgccgcgc ttgaagccga tcacgcgctc gaccgtggtc
     1381 gcatgcctga agcgccattg gcggaacgcg tcctcgaggt ccaccagctt ctcggcgagc
     1441 tcatagaggt cccagttctc ggcggtcgag cggtagaccg cgacccatgc ctcgcacacc
     1501 gattcgttga aggcgtatgc cacgcgccaa tcgcgctcga cgcattcgcc gtcgatcctg
     1561 aatccgcggc gccccaggag ccgtatggct tcatcgtata gcgacggctc acgatgcagc
     1621 gcgtcgagct ccgcgaagat ctcggcgcga tggcggtgcg gctccatccg gaccgcgtcc
     1681 ttgtttccga ggaggaactc gatggcgcga tactggaacg actggaaccc cgaagccgcg
     1741 ccgaggtcat tcctgaacga cgagtactcc gcgggggtga gggtggcgag gacgtcccac
     1801 gattgattga gctgcgccat gacgcgggcc acgcgcgcga gcatcttgaa ggcggcccgc
     1861 tggatgatga agagcatctc gttgtggtcg cgcgagcgcg gctgctgcgc ggcgagcatc
     1921 cggtcgaggt gcaggtagtc gccgtagctc atcgcctccg agaagtcgag gcgcgcgccg
     1981 tcggcggcgg aaggggcttt gtcttgggcc atggggcttg taaggctagt ctagcctcgt
     2041 tcgactcacc ggggattggg taagatgcct ccagcaaaca ccgaccatca tgcacgcgac
     2101 gcttccgctc ctcgttgaaa ccgatttccc gccgctcggg cgccgtctcc tcgacacgct
     2161 gcaggtgaac gtcggatacg tatgcaacca gacgtgcctt cactgccacg tgaacgccgg
     2221 gccgacgcgc accgagtcga tgacgcgcga gacggcggac caggtgctcg agtacctcgc
     2281 ggcgagcggc gcgaccacgc tcgatctcac gggcggcgcg cccgagctca atgcgcactt
     2341 ccgccatctc gtcaccgggg cgcgctcgct cggggcgcgc gtgatcgacc ggtgcaacct
     2401 caccatcctc ttcgagccgg gtcaggagga tctcgcgcgg ttcctcgcgc tgaatcgcgt
     2461 cgagatcgtc gcgtcgcttc cctgctacac cgcggagctc gtcgatcgcc agcgcggcga
     2521 cggggtgtac gagcgcagca tcgccgccat ccggatcctc aacgatctgg gctacgggcg
     2581 cgacgggagc gccctcaccc tcga
//
LOCUS       DASVII010000120         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_77767, whole
            genome shotgun sequence.
ACCESSION   DASVII010000120 DASVII010000000
VERSION     DASVII010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77767"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VF309_01555"
     CDS             complement(<1..1045)
                     /locus_tag="VF309_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465297.1"
                     /translation="MAGDLVHNSARVLEFDALRDLLRGYTSSPLGHERIAALAPSTDR
                     EWIESQQELASEIREFRRVGGRFDFSALIDIGLLVEKSRIVGAALETTDIRDVILVVD
                     RAAEWREIALHPPANMNSDWHGVRALSDQIVDFTEFLRGFRNKIQPDGTLEDKASPEL
                     ARIRREIERQRRTIQESLRGYLRRLAEGGTVQDELVTIRGERFVIPVKVEQKRRVQGV
                     VHGASSSGQTVFVEPLETIEQNNELVRLLDEELAEIHGILLEMTQRIGAHAEAILAAV
                     DVLAELELQFAKARFAENYNCVAVTLSEDTSNQQGSEGSSRQDSCRDGRLGRPASAAS
                     LLLHNARHPLLERN"
     gene            complement(1143..>1653)
                     /locus_tag="VF309_01560"
     CDS             complement(1143..>1653)
                     /locus_tag="VF309_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7465298.1"
                     /translation="FAQQAPGEKVPVDLSILLHRAVQMQESRHATGKIRLTVSIEENF
                     PRVQGNANQLFQACVEIIENAMDALADNGGGALQITAQRQGNEAVLQFSDSGPGVRDP
                     QRVFDPFYTTKPVGKGTGLGLRAVYGVIQEHNGQITCQNKPEGGALFVVRFPVAAAPV
                     VQVAGAAAI"
BASE COUNT          298 a          527 c          506 g          322 t
ORIGIN      
        1 gattgcgttc gagcagcggg tggcgtgcgt tgtgcagaag caggctcgct gcgctcgccg
       61 gacggccgag gcggccgtcc ctacatgaat cttgtcggct actcccctct gaaccttgtt
      121 gattcgatgt gtcctcactt agcgtcactg cgacgcagtt gtagttttcg gcgaagcgcg
      181 ctttggcgaa ctgcagctca agttccgcca gcacgtccac tgcggccaga atcgcttccg
      241 cgtgcgctcc gatgcgctgc gtcatttcca ggagaatgcc atgaatctcc gccagttcct
      301 cgtccagcag gcgcaccagt tcgttattct gttcgatcgt ctcgagcggc tcgacgaaca
      361 cggtttggcc gctggaactg gcgccgtgca ccactccctg cacgcggcgc ttctgctcga
      421 ctttgaccgg gatgacaaag cgttcgccgc ggatcgtgac cagctcatcc tgcacggttc
      481 cgccctccgc cagacggcgt aaataaccgc gcagggattc ctgaatcgtg cggcgctgtc
      541 tttcaatctc gcgacgaatt cgggccagct cgggagaagc cttgtcttcc agcgttccgt
      601 cgggttggat cttgttgcgg aacccgcgca ggaattcggt gaagtccaca atctggtccg
      661 agagagcgcg cacaccgtgc cagtcggaat tcatgttcgc cggaggatgc agggcgatct
      721 cgcgccactc cgccgcgcga tcgaccacca ggatcacgtc gcgaatgtcc gtggtctcga
      781 gcgcggctcc gacgatgcgt gatttctcga cgaggaggcc gatgtcgatc agcgcggaga
      841 aatcaaagcg accgcccacg cgccggaatt cccgaatctc gctcgccagc tcctgctggc
      901 tttcaatcca ttcacggtcg gtcgagggcg cgagcgcggc aatgcgctca tgcccgagcg
      961 gggaagaggt gtatccgcgg agaaggtccc gcagagcatc gaactccagc acgcgcgccg
     1021 agttatgaac gaggtcacca gccatttcaa tttatgttac aggagtggga cttcgcccgg
     1081 aggcgcaacg cgggaaggag cgacggtaaa ggaaggaaag atgcgccgca ggcaaacacc
     1141 gatcagatcg cggccgcccc ggccacttgc accactggcg ccgcagccac tggaaaccgc
     1201 accacgaaga gcgccccgcc ttccggcttg ttctggcagg taatctgccc gttgtgttcc
     1261 tggatgacgc cataaacggc cctcaggccc aggccggttc ccttgcctac gggcttggtc
     1321 gtgtagaacg gatcgaaaac gcgctgcggg tcgcgaaccc cgggaccgga atcggagaac
     1381 tgcagcacgg cctcgttgcc ttgccgttgc gcggtgattt gcagcgcgcc gccgccgttg
     1441 tcagccagcg cgtccatggc gttctcgatg atctcgacac aggcctgaaa tagctggttg
     1501 gcgttgccct gtacccgcgg aaagttctcc tcgatcgata ccgttaagcg gatcttgccg
     1561 gtcgcatgcc gcgactcctg catctgtaca gcgcggtgca gcaggatgct caggtccacc
     1621 ggcacctttt cgcccggggc ctgctgggca aaa
//
LOCUS       DASVII010000121         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_581313,
            whole genome shotgun sequence.
ACCESSION   DASVII010000121 DASVII010000000
VERSION     DASVII010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581313"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..735
                     /locus_tag="VF309_01565"
     CDS             163..735
                     /locus_tag="VF309_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX7465299.1"
                     /translation="MKTIVYLHGFVSSPQSKKAVMLGDYLKNIMSGIDYRVPALTDRP
                     ARAVEQAREACAGVAAQDLTIVGSSLGGFYATVLAERLGCRAALLNPAVRPHLHFDRH
                     LGPQRNLYTGEEFVLGREHIDELEDLAPREISRPGRYWLFVETGDVVLDYREAVAFYR
                     GALQTVVRGGDHTLASFPEHVPELVEWARS"
     gene            749..2581
                     /locus_tag="VF309_01570"
     CDS             749..2581
                     /locus_tag="VF309_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX7465300.1"
                     /translation="MKQVLFEEDGAFRVGTILGESGASFQVEAAHGKRSKVKAASILM
                     RFDGQSLTAFMPQAQKLAEPIDPQFLWEVCGADEFGFDALARDYFGRAPSPQEAVAVA
                     LALHSHPMYFYKRGKGRYQGAPETNLKAALAGAEKKRRQQEQVDAWAAELAAGRVPEP
                     LAGRLDALLFKPDKMSLEWRALDAAATALGMTPQRALAAAGALSGPEDYFLRRFAFEL
                     FPKGLGFPDIAPAAMPADLPEAAMPAFSIDDAETTEIDDAFSVQRLEDGRLRVGVHIA
                     APALLFDRGHAVEAIARERLSTVYFPGGKITMLPPGAVEAATLARGRRVAAASLYLTL
                     DAAGTGILDSTSRLEWVAIAENLRLADLDARMGDEAVASGRIEGPHGEDLFVLWRLAR
                     ALKALRGAGEERADRLDFNIRVVDGRVSIDPRRRGSPVDTLVAELMIHVNSAWGKLLA
                     ERGFDAIYRNQKGAKTRMEVEPGAHEWLGVSHYAWASSPLRRFSDLANQRQLAAALRA
                     EEPAYSREELVQAARDFETAYEAYAEHQRLLERYWCLKYLLQEGIESADAVVLREELV
                     RIDGMPLVCRAIGLPASPPGTRVRVSFGEVDLWEAAVLTRYAGK"
     gene            2686..>3112
                     /locus_tag="VF309_01575"
     CDS             2686..>3112
                     /locus_tag="VF309_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7465301.1"
                     /translation="MLERFGLVATMQVAMIASIAFHAFLVLGLGFKMISAGGLDEPHN
                     VMDVVLVNSKSAARPDKADALAQANLDGGGNTDQKLRAKSPFPAVDPKDPSPEMKAAQ
                     SRVKELEAEAKELMISLKSKAVVAQAAPPLAGPAQSDAEA"
BASE COUNT          458 a         1102 c         1081 g          471 t
ORIGIN      
        1 tctcgcaggg agaggccgtg caccggccgt cgacgctgta cctgtcggtg aaatacgggc
       61 ccgcgtcgcc ggcggacccc aggatcctgg tgggcggcga cgtgatagag ctcgggcgag
      121 gcgcggtgag cctgtgagcc gcgcgcgagg ccctgcggcc gcttgaagac gatcgtctac
      181 ctgcacggct tcgtgagctc gccacagtcg aagaaagccg tgatgctcgg ggactacctg
      241 aagaacatca tgtccgggat cgactatcgg gttcccgcgc tcaccgaccg tccggcgcgc
      301 gccgtcgagc aggcgcgcga ggcctgcgcg ggggtcgccg cccaggacct cacgatcgtc
      361 ggcagctcgc tgggcggctt ctacgcgacg gtgctggccg agcgcctcgg ctgccgcgcc
      421 gcgctcctga atcccgccgt gaggccgcac ctgcacttcg accgccacct cgggccgcag
      481 cgcaatctct atacgggaga ggaattcgtc ctgggccgcg agcacatcga cgagctggag
      541 gacctcgcgc cccgcgagat ctcgcgtccc gggcgctact ggctcttcgt cgagacgggc
      601 gacgtcgtcc tcgactatcg cgaggcggtc gcgttctatc ggggagcgct gcagaccgtg
      661 gtgcgcggcg gcgatcacac gctcgcctcc ttccccgagc acgttcccga gctggtcgaa
      721 tgggcgcgat cgtgatgcgc gacccaccgt gaagcaggtt ctcttcgagg aggacggcgc
      781 gttccgcgtg ggcacgattc tcggggagag cggcgcctcc ttccaggtcg aggccgcgca
      841 cggcaagcgc tccaaggtga aggcggcgtc gatcctcatg cgcttcgatg gccagtcgct
      901 caccgccttc atgccgcagg cccagaagct cgccgagccg atcgatccgc aattcctgtg
      961 ggaagtgtgc ggcgcggacg agttcggctt cgacgcgctc gcgcgcgatt acttcggccg
     1021 cgcgccctcg ccgcaggagg ccgtcgcggt cgccctcgcg ctgcattcgc acccgatgta
     1081 cttctacaag cggggcaagg ggcgatacca gggcgcgccc gagaccaacc tcaaggcggc
     1141 gctcgcgggc gcggagaaga agcggcgcca gcaggagcag gtggatgcct gggccgccga
     1201 gctcgcggcg ggacgcgtgc ccgagccgct cgcgggcagg ctcgacgccc ttctcttcaa
     1261 gcccgacaag atgagcctcg aatggcgcgc gctcgacgcc gccgctaccg cgctcggcat
     1321 gacgccgcag cgcgccctcg cggccgcggg cgctctctcc gggcccgaag actatttcct
     1381 gcggcgcttc gccttcgagc tcttccccaa gggcttggga tttccggaca tcgcgccggc
     1441 cgcgatgccc gccgacctgc cggaagcggc gatgcccgcg ttctccatcg acgacgcgga
     1501 gacgacggag atcgacgacg cgttctcggt gcagcggctc gaggacggcc ggttgcgcgt
     1561 cggcgtgcac atcgccgcgc ccgcgctcct cttcgaccgc ggacacgccg tcgaggcgat
     1621 cgcgcgcgag cgcctgtcca ccgtgtattt ccccggcggc aagatcacaa tgctcccacc
     1681 gggcgccgtg gaagccgcca cgctcgcccg cggccgccgc gtggccgcgg cctccctcta
     1741 cctcacgctc gacgccgccg gcaccgggat cctggactcg acctcgcgcc tcgagtgggt
     1801 ggcgatcgca gagaacctgc gccttgccga tctcgatgcg cgcatgggcg acgaggcggt
     1861 ggcctcgggc cggatcgaag gcccccatgg cgaggacctc ttcgtgctgt ggcgcctcgc
     1921 gcgcgcgctg aaggcgttgc gcggcgccgg cgaggagcgc gccgatcgcc tcgacttcaa
     1981 catccgcgtc gtcgacgggc gcgtctccat cgacccgcgc cggcgaggct cgcccgtcga
     2041 cacgctggtc gccgagctca tgatccatgt gaactccgcg tggggaaagc tcctcgcgga
     2101 gcggggcttc gatgcgatct atcgcaacca gaagggcgcg aagacgcgca tggaagtcga
     2161 gccgggcgcc cacgagtggc tcggcgtctc gcactacgcg tgggcgagct ctccgctgcg
     2221 ccgcttctcg gacctcgcca accagcgaca gctggcggcg gcgttgaggg cagaggagcc
     2281 cgcgtattcg cgcgaggagc tcgtgcaggc ggcgcgcgat ttcgagacgg cctacgaggc
     2341 ctatgccgag caccagcgcc tcctggagcg ctattggtgc ctcaagtacc tcctccagga
     2401 ggggatcgag tccgccgacg cggtggtcct gcgcgaagag ctggtgcgca tcgacggcat
     2461 gccgctggtt tgccgcgcca tcgggctccc ggcatcgcct ccgggaacgc gcgtgcgcgt
     2521 gagcttcggc gaagtcgacc tctgggaggc cgcggttctc acccgctacg cggggaaatg
     2581 accgtacaat tcccggtcat ggcaagtccc gcgcgctctt cgtaattcct gccgatgcag
     2641 ttgaggcagc cctcccccga cgccctcgtc tccttttcag acttcttgct cgagcgcttc
     2701 gggctggtcg cgaccatgca ggtggcgatg atcgcgtcga tcgcattcca cgcgttcctc
     2761 gtgctggggc tcggcttcaa gatgatcagc gcgggcgggc tcgacgagcc ccacaacgtg
     2821 atggacgtgg tcctcgtgaa ctcgaagagc gcggcgcggc cggacaaggc cgacgcgctc
     2881 gcgcaggcga atctcgacgg cggcggcaac accgaccaga agcttcgcgc caagagcccc
     2941 ttcccggccg tggacccgaa ggatccgagc cccgagatga aggccgcgca atcgcgcgtg
     3001 aaggagctcg aggcggaggc gaaggagctc atgatctccc tcaagtcgaa ggccgtggtg
     3061 gcgcaggccg cacccccgct cgcgggtccg gcgcagtccg acgccgaggc gc
//
LOCUS       DASVII010000122         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_473552,
            whole genome shotgun sequence.
ACCESSION   DASVII010000122 DASVII010000000
VERSION     DASVII010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473552"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VF309_01580"
     CDS             complement(<1..1579)
                     /locus_tag="VF309_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7465302.1"
                     /translation="MKVLVVEDDSETRRGVQMLLEQFGLDVKAASTLAEGRAALASFE
                     PDVCLTDLQLPDGDGIELIRVSRASDPGCEVVVLTGHGSLDTAVEAMKAGAFAYLVKP
                     LRGAQLEAVLERLRPTQDPGDGDAALWKALDETGRFGEMVGVSGAMREMCAVIARIAR
                     SDAPVMITGESGSGKEAAAQTIHALSRRRKNPLVAINCGAVSANLIESELFGHEKGAF
                     TGAEKRRAGYFELAQGGTLFLDEVTEMSLELQVKFLRVLETRSFRRVGGNEEIGSDLR
                     IVSSSNRDLLDAIEKKILREDLYYRLNVFPILLPPLRERAGDITLLAEHFVQKYARSF
                     GKAFKLLYASSGEQALELARENDIAAVVTDQRMPRMTGLELLRAVRDVRPDAIGIIVT
                     AFTDVDVLIESINLGRIYRYVTKPWDSKEMRGILTQAIERFHLGRENRRLSEQLAEYA
                     GMLASEAHGAFDFGVIVGDSPALREVLARVEQVAQTSATVLLRGETGTGKEMVARAIH
                     INSARETKPFIKVNCAAL"
     gene            complement(1576..>1783)
                     /locus_tag="VF309_01585"
     CDS             complement(1576..>1783)
                     /locus_tag="VF309_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465303.1"
                     /translation="DGAVSFSFRVPLAHGAPGDMLFKTRSRPDAGLGPFLARWTFEAH
                     GGRLEGAGDATHLTVAGSLPLADA"
BASE COUNT          279 a          610 c          605 g          289 t
ORIGIN      
        1 ccagcgccgc gcagtttact ttgatgaagg gcttggtctc gcgggcgctg ttgatgtgga
       61 tggcgcgggc caccatctcc ttgccggtgc cggtctcgcc gcgcaagagg accgtggcgc
      121 tggtctgggc gacctgctcc acgcgcgcca ggacctcgcg cagcgcgggc gagtcgccca
      181 cgatcacgcc gaagtcgaac gcgccatggg cctcgctggc cagcattccg gcgtactcgg
      241 cgagctgctc cgacagccgc cggttctcac ggccgaggtg aaagcgctcg atggcctggg
      301 tcaggatgcc gcgcatctcc ttcgagtccc agggcttggt gacatagcga tagatgcggc
      361 cgaggttgat cgattcaatc aacacgtcga catccgtgaa cgcggtgacg atgatgccga
      421 tggcgtcggg gcggacgtcc cgcaccgcgc gcaggagctc gaggccggtc atgcgcggca
      481 tacgctggtc ggtcacgacc gcggcgatgt cgttttcgcg ggcgagctcg agggcctgct
      541 cgccgctcga cgcgtagagg agcttgaagg ccttgccgaa gctgcgggcg tacttctgca
      601 cgaagtgctc ggccagcaag gtgatgtcgc cggcgcgctc gcgcaggggc ggcagcagga
      661 tggggaacac gttcagccgg tagtagaggt cctcgcgcag aatcttcttt tcgatggcgt
      721 cgagaaggtc gcgattcgag ctcgaaacga tgcgcaggtc gctcccgatc tcctcgttgc
      781 cgcccacgcg gcggaacgac cgggtctcga gaacacgcag gaacttgacc tggagctcca
      841 gcgacatctc ggtcacctcg tccaggaaaa gagtgcctcc ctgcgcgagc tcgaagtagc
      901 cggcccggcg cttctctgct ccggtgaacg ctcccttctc gtgcccgaag agctcgctct
      961 cgatgaggtt cgcggacacg gcgccgcaat tgatcgcgac gagaggattc ttgcgcctgc
     1021 gcgagagcgc gtggatcgtc tgggcggcgg cttccttgcc actgccgctc tcgcccgtga
     1081 tcatcacggg ggcgtccgac cgcgcgatcc gcgcgatgac ggcgcacatc tcgcgcatcg
     1141 cgccggagac acccaccatc tcgccgaacc ggcctgtctc gtccagcgcc ttccagagcg
     1201 ccgcgtcccc gtccccgggg tcctgcgtcg ggcgcaaccg ctcgagcacg gcctcgagct
     1261 gcgcgccccg caagggcttg acgaggtagg cgaaggcgcc cgccttcatc gcctcgacgg
     1321 ccgtatcgag tgatccgtgg ccggtcagca ccacgacctc gcatccggga tcggacgcgc
     1381 gcgagacccg gatgagctcg atgccgtcgc cgtccgggag ctggaggtcc gtcaggcaga
     1441 cgtcgggctc gaacgacgcg agcgccgcgc gtccctccgc gagcgtcgac gcggctttca
     1501 cgtcgaggcc aaattgctcg aggagcatct ggactcccct tcgggtctcg gagtcgtctt
     1561 cgacgaccag aaccttcatg cgtccgccag gggcagggat cccgccacgg tcagatgcgt
     1621 tgcgtccccg gcaccctcga gccggccccc gtgggcctcg aaagtccagc gcgcgagaaa
     1681 agggccgagc cccgcatccg ggcggctgcg cgtcttgaac agcatgtcgc cgggcgcgcc
     1741 gtgcgcgagt gggacgcgaa acgaaaacga taccgctccg tcc
//
LOCUS       DASVII010000123         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_114114,
            whole genome shotgun sequence.
ACCESSION   DASVII010000123 DASVII010000000
VERSION     DASVII010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114114"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VF309_01590"
     CDS             complement(<1..842)
                     /locus_tag="VF309_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7465304.1"
                     /translation="MKRTWRMLIAACAALALGAAAETEISFYYPVAVGGPITKTIDQM
                     TADFEKDNPGIKVKPIYAGTYQETIVKAITANKAGTPPTTAILLSTDMFTLIDEDAIV
                     PFDELAASADDKAWMKSFYPAFMANSQTGGKTWGIPFQRSTIVLYWNKELFKEAGLDP
                     TRPPATWKEMLEDAQKLTKRDSSGNVTQYGVQIPSSGFPYWLFQGLTTPNGVELMNSA
                     GTETYFDKPAVIEALAFWVDLGRKHKVMPTGVVEWGTTPKDFFERKIAMMWTTTGNLT
                     NVRN"
     gene            complement(839..1816)
                     /locus_tag="VF309_01595"
     CDS             complement(839..1816)
                     /locus_tag="VF309_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465305.1"
                     /translation="MSSIRLEQIAKHWGESRALDGITFSAEPGSFVVLLGPSGCGKST
                     TLRLIAGLDTPTAGTIRIGERDVTHLAPAQRGISMVFQSYALFPHLTVAENIVFGLRV
                     RKVAGRDAKLKRVAEMLGLMPLLARKPSQLSGGQQQRVALGRALVAEAPVCLMDEPLS
                     NLDAQLRGEMRREIRSLQQRLGITMVYVTHDQTEAMTMADRVVLLRNGRIEQVGSPED
                     LYARPATAFTAGFIGAPPMNLFALNGGGALTGVRPEDMRLAAEGLEASVESTEYLGAD
                     SLVAAKAGADSILVRVPGHARVKKGDAVRLAWDASHEHHFDAQTGGRKP"
BASE COUNT          288 a          605 c          626 g          348 t
ORIGIN      
        1 ttgttcctaa cgttggtgag gttgcccgtg gtggtccaca tcatcgcgat cttcctctcg
       61 aagaaatcct tcggcgtcgt gccccactcg accacgcccg tgggcatcac cttgtgtttg
      121 cggccgaggt ccacccagaa ggcgagggcc tcgatcaccg cgggcttgtc gaagtaggtc
      181 tcggtgcccg cggaattcat gagctccacg ccattgggcg tcgtgaggcc ctggaagagc
      241 cagtatggaa atcccgacga cgggatctgc acgccgtact gcgtgacgtt gcccgaggaa
      301 tcgcgcttcg tgagcttctg cgcgtcctcg agcatctcct tccacgtcgc cggcgggcgg
      361 gtcggatcga ggcccgcttc cttgaagagc tccttgttcc agtagaggac gatcgtcgag
      421 cgctggaagg gaatgcccca ggtcttgccc cccgtctggc tgttcgccat gaacgcggga
      481 tagaagctct tcatccacgc cttgtcgtcg gcgctcgcgg cgagctcgtc gaacggcacg
      541 atcgcgtcct cgtcgatcaa cgtgaacatg tcggtggaaa ggaggatcgc ggtggtgggc
      601 ggcgtccccg ccttgttcgc ggtgatcgcc ttcacgatcg tctcctggta ggtccccgcg
      661 tagatgggct tcaccttgat gccgggattg tccttctcga agtccgcggt catctggtcg
      721 atggtcttgg tgatgggccc gcccaccgcg accgggtagt agaacgagat ctcggtttcg
      781 gccgcggcgc cgagcgccag cgcggcgcag gccgcgatca acatccgcca ggttcttttc
      841 atggttttct tcctcctgtt tgtgcatcga aatgatgttc gtgcgacgca tcccaggcaa
      901 ggcgcaccgc gtcgcccttc ttcacccggg catggccggg aacgcgcacc aggatcgagt
      961 ccgcgccggc cttcgccgcg acgagcgagt cggcgccgag atactcggtg gattcgacgc
     1021 tcgcttcgag gccttcggcg gcgaggcgca tgtcctcggg acgcacgccc gtgagggcgc
     1081 cgccgccgtt cagggcgaac agattcatcg gcggcgcgcc gatgaagccc gcggtgaacg
     1141 cggtcgcggg ccgcgcatag agatcctcgg gcgagccgac ttgctcgatg cgcccgttgc
     1201 gcagcagcac cacgcggtcg gccatcgtca tcgcctcggt ctggtcgtgg gtgacgtaca
     1261 ccatcgtgat gccgaggcgc tgctgcagcg atcggatctc gcggcgcatt tcgccccgca
     1321 actgcgcgtc gaggttcgac agcggctcgt ccatgaggca gacgggcgcc tcggcgacca
     1381 gggcgcggcc gagcgccacg cgctgctgct ggccgccgga aagctgcgag ggcttgcgcg
     1441 cgaggagcgg catgagcccg agcatctcgg ccacgcgttt cagtttcgcg tcacgcccgg
     1501 cgaccttgcg cacccgcagg ccgaagacga tgttctcggc gaccgtgagg tgcgggaaga
     1561 gcgcatagga ttggaacacc atcgagattc cgcgttgggc gggcgcaagg tgggtgacgt
     1621 cgcgttctcc gatgcggatg gtccccgcgg tgggcgtatc gaggcccgcg atcagcctca
     1681 aggtggtcga cttgccgcag cccgagggac ccagcaggac cacgaacgag cccggctcgg
     1741 ccgagaaggt gatcccgtcc agcgcgcgcg actcccccca gtgcttcgcg atttgttcga
     1801 gacggatgga tgacaccggc gcattttctc ctaaaatggc ggcgagccct ttgatccccg
     1861 ccgaaag
//
LOCUS       DASVII010000124         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_365906,
            whole genome shotgun sequence.
ACCESSION   DASVII010000124 DASVII010000000
VERSION     DASVII010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365906"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VF309_01600"
     CDS             <1..948
                     /locus_tag="VF309_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX7465306.1"
                     /translation="GGAFGSARYKLKGVEKGAHEYERLIEVFKAHDIGYFFYNGGNDS
                     ADTCPGFGSVAKYVATSMREAGLDVESMAKTSTKVFILEVMGRHAGWITAATGLAQRA
                     AGQGPHILLFPEVPWNEEAFLARVDEAVKAHGYCAVGASEGIQDKDGKFLSESGLKDA
                     FGHSQLGGVAPMLANLVQSRLKYKQHWAVADYLQRAARHIASKTDLEQSYAVGKAAVE
                     LAAKGMSGVMPTIVRTSGRPYRWKIGVARLTDVANVEKKMPRDFITADGFHITAKCRN
                     YLQPLISGEAFPPFENGLPKYVRLKNLAVRKKTHSDFKL"
     gene            988..>2012
                     /locus_tag="VF309_01605"
     CDS             988..>2012
                     /locus_tag="VF309_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX7465307.1"
                     /translation="MLNNAILIALACGAIALVYGGFTAAWILRQPDGNDRMREIAAAI
                     QTGAKAYLNRQYTTIGVVGVVLFIVLGFVPGLGWVSACGFAIGAIASGAAGYIGMNVS
                     VRSNVRTAEAARHGIGPALDIAFKGGAITGLLVVGLGLIAVSGFFMYLMGTSAHDARM
                     SDIVKPLIGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIA
                     DNVGDNVGDCAGMAADLFETYAVTIIATMVLAALLFPSPATVGNAVTYPLVLGAVSII
                     ASIVGCFFVKAAQGGKIMNALYRGLAVAGILSLVAFYFVTDWMMRDVIAGYPGMTVMK
                     LWGTCVVG"
BASE COUNT          344 a          672 c          668 g          328 t
ORIGIN      
        1 ggcggcgcgt tcggatcggc ccgctacaag ctgaaaggcg tcgagaaggg cgcgcacgag
       61 tacgagcgcc tcatcgaggt tttcaaggcg cacgacatcg gctacttctt ctacaacggc
      121 ggcaacgact ccgccgacac gtgccccggt ttcggctcgg tcgccaagta cgtcgccacc
      181 agcatgcgcg aggcggggct cgacgtcgaa tccatggcga agacctcgac caaggtcttc
      241 atcctcgagg tgatgggacg ccacgcggga tggatcaccg cggccacggg cctcgcgcag
      301 cgcgccgccg ggcagggacc gcacatcctc ctctttcccg aggtgccgtg gaacgaggag
      361 gccttcctcg cgcgcgtcga cgaggcggtg aaagcgcacg gctactgcgc ggtcggcgct
      421 tccgaaggca tccaggacaa ggacggaaag ttcctttcgg aatcgggact gaaggacgcg
      481 ttcggccact cgcagctggg tggcgtggcg ccgatgctcg ccaacctcgt gcagtcccgg
      541 ctcaagtaca agcagcactg ggcggtggcc gattacctcc aacgcgccgc gcgccacatc
      601 gcctcgaaga ccgacctcga gcagtcctat gcggtgggca aggccgcggt cgagctcgcc
      661 gcgaagggca tgtcgggcgt gatgccgacc atcgtgcgca cgtcgggcag gccctatcgc
      721 tggaagatcg gcgtcgcgag gctcaccgac gtcgccaacg tcgagaagaa gatgccgcgc
      781 gacttcatca ccgcggacgg cttccacatc acggcgaagt gccgcaatta tctgcagccg
      841 ctcatctcgg gcgaggcgtt ccctcctttc gagaacgggc tgccgaagta cgtacgactg
      901 aagaacctgg ccgtgcgcaa gaagacgcac agtgatttca agctgtgacg acgcagtgac
      961 gcactcaaaa aaacctggga gaagcagatg ttgaacaacg caattctcat agcgctggca
     1021 tgcggcgcga tcgccctcgt gtacgggggc ttcaccgccg cgtggatcct gcgccagccc
     1081 gacggcaacg accgcatgcg cgagatcgcc gcggccatcc agaccggcgc gaaggcgtat
     1141 ctcaaccggc aatacacgac catcggcgtg gtgggcgtgg tcctcttcat cgtgctcggg
     1201 ttcgtcccgg gcctcggatg ggtgagcgcg tgcggattcg ccatcggcgc gatcgcctcg
     1261 ggcgcggccg gatacatcgg catgaacgtc tcggtgcgct ccaacgtgcg caccgcggaa
     1321 gcggcacgcc acgggatcgg ccccgcgctc gacatcgcct tcaagggcgg cgccatcacg
     1381 ggcctgctcg tggtcggcct gggcctgatc gccgtgtcgg gattcttcat gtacctcatg
     1441 ggcacctcgg cgcacgacgc gcgcatgagc gacatcgtga agccgctcat cggcctcgcc
     1501 ttcggctcct ccctcatctc gatcttcgct cgcctcggcg ggggcatctt caccaagggc
     1561 gccgacgtgg gcgcggacct ggtggggaag gtcgaagccg gaattcccga ggacgatccg
     1621 cgcaaccccg cggtcatcgc cgacaacgtg ggcgacaacg tgggcgactg cgccggcatg
     1681 gcggccgacc tcttcgagac ctacgcggtg accatcatcg cgacgatggt gctcgccgca
     1741 ctcctcttcc cgagccccgc gacggtcggc aacgcggtga cctatccgct ggtgctgggc
     1801 gccgtctcga tcatcgcgtc gatcgtcggc tgcttcttcg tgaaggccgc gcagggcggc
     1861 aagatcatga acgcgctcta ccgcgggctc gcggtggcgg gcatcctctc gctggtcgcg
     1921 ttctacttcg tcaccgactg gatgatgcgc gacgtgatcg cgggctaccc gggcatgacc
     1981 gtgatgaagc tctggggcac gtgcgtcgtg gg
//
LOCUS       DASVII010000125         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_257890,
            whole genome shotgun sequence.
ACCESSION   DASVII010000125 DASVII010000000
VERSION     DASVII010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257890"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..>1633
                     /locus_tag="VF309_01610"
     CDS             8..>1633
                     /locus_tag="VF309_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7465308.1"
                     /translation="MDRGFDFIKRTYERALDWTLGTRPAVYAVWIVLTLLIAPMYMFS
                     PAELAPNEDQGGVFTAMDVPPNATLERLMPYTEAINKQFLATPEFDHSFQITFPTGGF
                     GGMLVKPWEERKRSIFPIQEGLQKELGAITGVRAPVFLPAALPSAGTFPVEFVLSSTS
                     SHEEMLRVAQQLVQEAVKSGLFAFPPVTDVRIDQAKSEIVIDRDKVASMGLSLQQVGA
                     DLSSMLGGNFVNRFNIDGRSYKVIAQIERAGRLTTDQLNDIYISGPNGRLMPLSAIAT
                     IRQGVEPRTLNRFQQLNAIKISGLAPRGLDGGLRVLEDSAAKILPQGYRIDYTGESRQ
                     LRQESGKFLPAMGLALLLIFLVLAAQFNSFRDPLVILAGSVPLAMFGAMIFTFLKFAG
                     PPGLHFKLTEGWTTTLNIYSQVGLVTLVGLVSKNGILIVEFANAQQQKGLSKLDAVRA
                     AAATRLRPILMTTVATVAGHFPLTLVTGAGAVARNSIGVVLVGGMSIGTLFTLFIVPS
                     LYVLLAKDHRGERAKVGDPALEANGGRKTELLAE"
BASE COUNT          297 a          556 c          477 g          303 t
ORIGIN      
        1 gcgagccatc gatcgcggtt tcgatttcat caagcgaacc tacgagcgcg ctctcgattg
       61 gacgctcggt acgaggcccg ccgtttacgc ggtgtggatc gtcctcacac tcctcattgc
      121 acccatgtac atgttctcgc cggccgagct ggcgccgaac gaggatcagg gcggtgtctt
      181 tactgccatg gacgtgccgc cgaacgcgac gctcgagcgg ctcatgccgt acaccgaggc
      241 catcaacaag cagttcttgg cgacacccga attcgaccac agcttccaga tcaccttccc
      301 gaccggcggc ttcgggggaa tgctggtgaa accatgggaa gagcgaaagc ggagcatctt
      361 tcccatccag gagggcctcc aaaaagagct cggcgccatc accggcgtcc gcgcgccggt
      421 cttcttgccc gccgccttgc caagcgcagg aacattcccc gtcgaattcg tcttgtcatc
      481 gacctcgagc cacgaagaga tgcttcgcgt cgcacagcag ctggttcaag aggccgtcaa
      541 gagcggcctc ttcgcgtttc ctcccgtgac cgacgtgcgc atcgatcaag ccaaatccga
      601 gatcgtcatc gatcgcgaca aggttgcctc gatgggcctc agcctccagc aggtgggcgc
      661 cgatctgtca tcgatgctag gcggtaactt cgtcaatcga ttcaacatcg acggccgcag
      721 ctacaaggtc atcgctcaga tcgagcgcgc aggtcggctc acgaccgatc agctgaacga
      781 catctacatc tccggtccga acgggcgact catgcctctc agcgccatcg cgaccattcg
      841 gcaaggggtc gagccgcgca cgctgaaccg ctttcagcag ctcaacgcga tcaagatctc
      901 aggcctagct ccgcgcggcc tcgacggagg cctgcgcgtt ctcgaggaca gcgcggccaa
      961 gattctgccg cagggctacc gcatcgacta cacgggagag tctcgacagc tgcgccagga
     1021 gtcggggaaa ttcctccccg ccatggggct ggcgctcctg ctcatcttcc tggtgctcgc
     1081 tgcccagttc aactcgttcc gcgatccgct ggtcatcctc gccggctcgg tgccgctcgc
     1141 catgttcggc gcgatgatct tcaccttcct gaagttcgcg ggtccgcccg gcctccactt
     1201 caagctgacc gaaggctgga cgaccaccct caacatctat tcgcaagtag ggctcgtcac
     1261 gctggtgggt ctggtgtcga agaacggcat tctgatcgtg gaattcgcca acgcccagca
     1321 gcagaagggg ctgagcaagc tcgacgcggt tcgggccgcg gccgccacgc gtctccgacc
     1381 cattctcatg accaccgttg ccaccgtggc cgggcacttt ccgctcaccc tggtgacagg
     1441 cgccggcgcc gtcgcgcgca actcgatcgg cgtcgtcctc gtcggcggaa tgagcattgg
     1501 aacactcttc acgctgttca tcgtgccgtc gctgtacgta ctgctcgcca aggatcaccg
     1561 cggcgagcgt gcgaaggtgg gcgaccccgc cttggaggcg aacggcggtc gcaagaccga
     1621 gctcctcgcc gag
//
LOCUS       DASVII010000126         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_545668,
            whole genome shotgun sequence.
ACCESSION   DASVII010000126 DASVII010000000
VERSION     DASVII010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545668"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VF309_01615"
     CDS             complement(<1..611)
                     /locus_tag="VF309_01615"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX7465309.1"
                     /translation="MTLYVPKHFRIDDRAALLAFVEKYAFGTLVSSGPGGLHVSHIPF
                     AVDRGDDGSARLLGHVARGNDQWAALEGAQHVVAIFQGPHAYVSPTWYATHPSVPTWN
                     YAVVHVHGKARLMDEAELHDLLMRLSATYEAPNAKPWRMSELPVEYVSTMLKAIVGFE
                     IEIERLEGKFKLSQNRPAEVPRVAAALEKGGEPQLAALMREHSP"
     gene            complement(608..1291)
                     /locus_tag="VF309_01620"
     CDS             complement(608..1291)
                     /locus_tag="VF309_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7465310.1"
                     /translation="MRALILAAGRGERMRPLTDSLPKPLLKVGGRALIDWQVERLARA
                     GFTDLVVNHSHLGAMIEESLGDGARFGARIRYSPESPALETAGGIAKALDLLAGEPFV
                     VVSSDIHTDFDYATLRGRMEAIARDPASCAIHLVLVDNPPWHARGDMGLRDGRVTRGE
                     PWLTYGNIAVFHPAVFREIAPGTSLRLFPWVYRFVEEGRVTGEHYRGPWDNVGTAAQL
                     AALDTRLSQ"
     gene            complement(1291..>1952)
                     /locus_tag="VF309_01625"
     CDS             complement(1291..>1952)
                     /locus_tag="VF309_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7465311.1"
                     /translation="ATSALVRWQRASKPGVLPEYDEALMRRELALFPEWYVGRHLGVA
                     LSARQKESLEDVFRAILARNLAEPRVYVHRDFMPRNLMVATPNPGILDFQDAVHGPIS
                     YDVASLFRDAFISWDEERILDGTIRYWEKARRASLPVHADFAELWRDAEWMGLQRHLK
                     VLGIFARLHYRDGKQGYLEDTPRFVGYVRRAVSRYDDLAPLGRLMDELEGARPEARRT
                     P"
BASE COUNT          309 a          649 c          701 g          293 t
ORIGIN      
        1 ggtgagtgtt cgcgcatcag ggcagcgagc tgcggctcgc cgcccttctc gagcgccgcg
       61 gccacgcgcg gcacttccgc gggacggttc tgggagagct tgaatttccc ctcgaggcgc
      121 tcgatctcga tctcgaagcc gacgatggcc ttcagcatcg tggagacata ttcgacgggc
      181 agctcggaca tgcgccacgg cttcgcgttg ggcgcttcgt acgtcgcgga caagcgcatc
      241 agcagatcgt gcagctcggc ctcgtccatg aggcgcgcct ttccgtggac gtgcaccacg
      301 gcgtagttcc acgtcggcac cgacggatgg gtggcatacc aggtcggcga cacgtaggcg
      361 tggggcccct ggaagatcgc gaccacgtgt tgcgcgcctt cgagcgcggc ccactggtcg
      421 ttgccgcgcg cgacgtggcc cagcagccgc gcgctcccgt cgtcgccgcg atcgaccgcg
      481 aagggaatgt ggctcacgtg aagcccgccc gggccgctcg agaccagggt gccgaaggcg
      541 tatttctcga cgaaggcgag cagcgccgcg cggtcgtcga tgcggaagtg ctttggaacg
      601 tagagcgtca ttgcgagagc ctcgtgtcca gcgccgcgag ctgcgcggcg gtgccgacgt
      661 tgtcccaggg gccccggtag tgctcgccgg tgacgcgccc ctcctcgacg aagcgataga
      721 cccagggaaa gagcctgagg gacgtgcccg gcgcgatctc cctgaagacg gcgggatgga
      781 aaaccgcgat gttgccatag gtgagccacg gctcgccgcg cgtgacgcgc ccgtcgcgaa
      841 ggcccatgtc gccccgcgcg tgccagggcg gattgtcgac cagcacgaga tggatcgcgc
      901 acgacgccgg atcgcgcgcg atcgcctcca ttcgaccgcg cagcgtcgcg tagtcgaagt
      961 cggtgtggat gtcgctgctg accacgacga agggttcgcc ggcgagaagg tcgagcgcct
     1021 tggcgatgcc gcccgccgtc tcgagcgccg gcgattccgg tgaatagcgg atgcgcgcgc
     1081 cgaagcgcgc gccgtcgccg agcgattcct cgatcatcgc gccgagatgc gaatggttga
     1141 cgacgaggtc ggtgaagccg gcgcgcgcga ggcgctcgac ctgccagtcg atgagcgcgc
     1201 ggcctcccac cttcagaagc ggcttcggca aggagtcggt cagcgggcgc atgcgctcgc
     1261 cgcgtcccgc cgcgaggatc agcgcgcgca tcagggcgtg cgccgcgctt cgggccgcgc
     1321 gccctcgagc tcgtccatca ggcgccccag cggcgcgagg tcgtcgtagc gcgagaccgc
     1381 ccggcgaacg tagcccacga agcgcggggt gtcctcgaga tagccctgct tgccgtcgcg
     1441 gtagtgcagg cgcgcgaaga tgcccagcac cttgagatgg cgctgcaggc ccatccactc
     1501 ggcgtcgcgc cacagctcgg cgaaatcggc gtggaccgga agcgatgcgc gccgcgcctt
     1561 ctcccagtag cggatcgtcc cgtcgaggat gcgttcctcg tcccagctga tgaaggcgtc
     1621 gcggaagagg gaagccacgt cgtagctgat gggcccgtgg accgcgtcct ggaaatcgag
     1681 gatgcccggg ttcggggtgg cgaccatgag gttgcggggc atgaagtcgc gatgcacgta
     1741 cacgcgcggc tcggcgaggt tgcgcgcgag gatcgccctg aagacatcct cgagcgattc
     1801 cttctgtcgc gcagagagcg caacgcccag atggcgcccg acgtaccact cgggaaagag
     1861 cgcgagctcg cggcgcataa gcgcttcgtc gtactccggg agaacgcccg gcttgctcgc
     1921 gcgctgccag cgcaccagcg cggaagtcgc gt
//
LOCUS       DASVII010000127         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_185912,
            whole genome shotgun sequence.
ACCESSION   DASVII010000127 DASVII010000000
VERSION     DASVII010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185912"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VF309_01630"
     CDS             <1..858
                     /locus_tag="VF309_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465312.1"
                     /translation="LDFVLDPPVQDIPRLREDATLKVWEGFETRIVFLGFDQARDELL
                     YSNVKGRNPFKDRRVRMALWQAIDTQALKTQVMRGLSIPTGIPLPDPAGSALPASMEK
                     RPPYDPAAAKRLLAEAGYPAGFGFTLTCPNNRYINDEKICIALAGMWARAGLDVKVDS
                     LPRAQFFPKALKLDVSAFLWGWGSDSPDAIFTLKPVLHGRSASGAGNNNVGDYRNAAL
                     DQLIDAAATEMDARKRQEMVNRAIAIVQDEVLVIPLHRQVIPWLSRAGVSVVHRPNNM
                     LYLPWVKVP"
     gene            complement(863..1264)
                     /locus_tag="VF309_01635"
     CDS             complement(863..1264)
                     /locus_tag="VF309_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465313.1"
                     /translation="MTTIIPALALAGALLGAPAVATPGGDAVPAIAPTRTVNLDRPGA
                     LEAVAAANPGHYRKLVEILRVSGEIDCIAMPAKLYVSFGATGSCTAPNLLMTSFPAKR
                     HVRLLLEDTAYEANVVIRSSPGELVPAEGPR"
     gene            1366..1770
                     /locus_tag="VF309_01640"
     CDS             1366..1770
                     /locus_tag="VF309_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX7465314.1"
                     /translation="MKLDISATPALRTICFIGFLGMTAQLLVFYEPNFAVQIVEATWD
                     KAIHVLYFGTMAFLLWIVTDRRWPLAVCLVVAFIGAADETLQAYTPGRTSDLDDWIAD
                     AIGAAAALLIARRITNRGGARAAPVASRTGDE"
BASE COUNT          302 a          646 c          609 g          297 t
ORIGIN      
        1 ctcgacttcg tcctcgaccc tccggtgcag gacatcccgc gccttcgcga ggacgcgacg
       61 ctcaaggtgt gggaaggctt cgagacgcgc atcgtcttcc tcggcttcga ccaggctcgc
      121 gacgagctcc tctattcgaa tgtgaagggg cgcaatccct tcaaggaccg gcgcgtgcgc
      181 atggcgctgt ggcaggcgat cgacacgcag gcgctcaaga cccaggtgat gcgcggcctt
      241 tcgataccca ccgggatccc gctgcccgat cccgcgggaa gcgccttgcc cgcgtcgatg
      301 gagaagcgcc cgccgtatga ccccgcggcg gcgaagcgcc tgcttgccga ggccggatac
      361 ccggccggat tcggcttcac cctcacctgc cccaacaacc gctacatcaa cgacgagaag
      421 atctgcatcg cgctcgccgg aatgtgggcg cgcgccggcc tcgacgtgaa ggtcgactcg
      481 ctgccgcgcg cgcagttctt ccccaaggcg ttgaagctcg acgtgagcgc gttcctctgg
      541 ggatggggga gcgattcgcc cgacgccatc ttcacgctga agccggtgct gcacgggcgc
      601 agcgcctcgg gcgcgggaaa caacaacgtg ggcgactatc gcaacgccgc gctcgaccag
      661 ctcatcgacg cggccgccac cgagatggac gcgaggaagc gccaggagat ggtgaaccgc
      721 gcgatcgcga tcgtgcagga cgaggtgctg gtgatcccgc tgcatcgcca ggtgattccg
      781 tggctgtcgc gcgccggggt gagcgtcgtc caccggccca acaacatgct ctacctgccg
      841 tgggtgaagg tgccctgagg cgctacctgg gcccttcggc gggaaccaac tcgccgggcg
      901 agcttcgaat caccacgttc gcctcgtaag cggtgtcttc gagcagcagc cggacgtgcc
      961 gtttcgcggg aaagctcgtc atcagcaggt tgggcgcggt gcacgagccc gtggcgccga
     1021 agctgacgta cagcttcgcg ggcatcgcga tgcaatcgat ctcgcccgat acgcgaagga
     1081 tttccacgag cttgcggtag tgcccgggat tcgcggccgc gacggcttcg agcgcgccgg
     1141 gccggtcgag gttgacggtg cgtgtgggcg cgatcgccgg aacggcatcg cctccgggcg
     1201 tggccacggc tggtgcgcca agcagcgcgc cggcgagggc gagcgccgga atgatcgtgg
     1261 tcatgatccc tcccgcacgt gatggcgatg gcgcccattg tgcccctcag ccgctgcggt
     1321 aacaatgcgc atgaactcct gcctgccaaa ggaaaccgcg acccgatgaa gctcgacatc
     1381 tccgccactc ccgcgctgcg gacgatctgc ttcatcggct ttctcggcat gacggcccag
     1441 ctgctcgtct tctacgagcc caacttcgcg gtgcagatcg tcgaggcgac gtgggacaag
     1501 gcgatccacg tcctctactt cgggaccatg gccttcctgc tgtggatcgt tacagacagg
     1561 cgctggccgc tcgcggtgtg cctcgtcgtc gccttcatcg gcgccgccga cgagaccctg
     1621 caggcctata cacccgggcg cacgtccgac ctcgacgact ggattgcaga cgccatcggc
     1681 gccgcggcgg cgctactcat cgcgcgacgc atcacgaatc gcggcggcgc ccgcgccgcg
     1741 ccggtcgcct cgagaaccgg agacgaatag tcagccctcg tacccgtcca cgagctgcgc
     1801 cggcccggtc gaggcgcacg tcactcccgt gcccgtgatc gacagaaccg ctcc
//
LOCUS       DASVII010000128         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_6523, whole
            genome shotgun sequence.
ACCESSION   DASVII010000128 DASVII010000000
VERSION     DASVII010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6523"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..976)
                     /locus_tag="VF309_01645"
     CDS             complement(38..976)
                     /locus_tag="VF309_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465315.1"
                     /translation="MLLKTVTRTQGNNEALKDGTVKPRGFSFDFVEVDPLIDAFRRMV
                     RAREFDVSEMALTTYITARAHGKRITGLPIFLMRAFHHGAVKHNVKSGIVSPKDLEGR
                     QVGVNRGYTVTTGLWARSILAHQYGVDLDKITWVLSGDEHVAEYRPPANVVPIEKGKK
                     MGDMLASGELGAAIGVDIDHPDVKPLIAKPREAGFEALRATGHYPINHLMVVKDELLA
                     DHPALAAEIFEAFAEAKRLYVERLKAGKIAQPTAVDDMHKRVMEITGKDPLPYGIEPN
                     RAMLEEVTRSVLEQKIVTRPVTVEELFPRSTHALAG"
     gene            complement(967..1302)
                     /locus_tag="VF309_01650"
     CDS             complement(967..1302)
                     /locus_tag="VF309_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465316.1"
                     /translation="MSLWADTGASGAGFGAARKGPRHLDAVERLKDWTRGRFALAGEE
                     TVLVSEIASALPGFPPLETIVAFWTLDGTRHHFRVFKPVEGVDEDDLPPAWLKDSLAL
                     SQGVECACC"
     gene            complement(1324..>1523)
                     /locus_tag="VF309_01655"
     CDS             complement(1324..>1523)
                     /locus_tag="VF309_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465317.1"
                     /translation="MTLKSARGHSYKACELALQQLASKRFPLEKVTTHTFGLADVDLA
                     IKSVGGNGVPDVIHASLMPWK"
BASE COUNT          215 a          501 c          528 g          279 t
ORIGIN      
        1 tccgtgacgg tcattgcgaa tcctcctgtc ggtattatca acctgcgagg gcatgggtgc
       61 tgcgcgggaa cagctcctcg accgtgaccg gccgggtgac gatcttctgc tcgagcacgc
      121 tgcgcgtcac ttcctcgagc atcgcgcgat tgggctcgat gccgtagggc agggggtcct
      181 tgccggtgat ctccatcacg cgcttgtgca tgtcgtcgac cgccgtgggc tgcgcgatct
      241 tccccgcctt gaggcgttcc acgtaaaggc gcttggcctc ggcgaatgcc tcgaagattt
      301 ccgccgcgag cgcgggatgg tcggcgagga gctcgtcctt caccaccatg aggtggttga
      361 tcgggtagtg gccggtggcg cgcagcgcct cgaaccccgc ctcgcgcggc ttggcgatga
      421 gcggcttcac gtcgggatgg tcgatgtcca cgccgatcgc cgcgccgagc tctcccgagg
      481 cgagcatgtc gcccatcttc ttccctttct cgatgggcac cacgttggcc ggcggccggt
      541 actcggccac gtgctcgtcg cccgagagca cccaggtgat cttgtcgagg tccaccccgt
      601 attggtgggc gaggatcgag cgcgcccaga gcccggtggt gacggtgtag ccgcggttca
      661 cgccgacctg ccggccctcg aggtccttcg gcgacacgat ccccgatttc acgttgtgct
      721 tgaccgcgcc atggtgaaag gcgcgcatga ggaagatcgg cagcccggtg atgcgcttgc
      781 cgtgggcccg cgcggtgatg tacgtggtca gcgccatctc ggacacgtcg aactcgcgcg
      841 cgcgcaccat gcgccggaag gcgtcgatca acgggtcgac ctcgacgaaa tcgaacgaga
      901 agccgcgcgg cttgacggtg ccgtccttga gcgcctcgtt gttgccctgt gtgcgggtga
      961 cggttttcag cagcatgcgc attcgactcc ttgggacagt gccagcgaat ccttgagcca
     1021 ggcgggcggc aggtcgtcct cgtccacgcc ttccaccggc ttgaagaccc tgaagtggtg
     1081 ccgcgtgccg tcgagggtcc agaaggcgac gatcgtctcc agcggcggga agccgggcag
     1141 cgcgctcgcg atctcggaga cgagcacggt ctcttccccg gcgagcgcga agcgcccgcg
     1201 cgtccagtcc ttgaggcgct cgacggcgtc gaggtggcgg gggcccttgc gcgcggcgcc
     1261 gaaccccgcg cccgaagcgc ccgtgtcggc ccagaggctc atggcgtcat tccggcgcgc
     1321 gttctacttc cagggcatga gcgacgcgtg gatcacgtcg ggcacgccgt tgccgccgac
     1381 cgacttgatg gcgaggtcca cgtccgcgag gccgaaggtg tgggtcgtga ccttttcgag
     1441 cgggaagcgc ttggaggcga gctgctgcaa cgcgagctcg cacgccttgt agctgtgccc
     1501 gcgcgcgctc ttcagcgtca tga
//
LOCUS       DASVII010000129         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_42447, whole
            genome shotgun sequence.
ACCESSION   DASVII010000129 DASVII010000000
VERSION     DASVII010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42447"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..660
                     /locus_tag="VF309_01660"
     CDS             142..660
                     /locus_tag="VF309_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX7465318.1"
                     /translation="MIYQLGDRRPTFHGDYFVADNAAVIGSVVMGANASVWWNVTIRG
                     DNDLITLGENVNVQDGSVVHTDSGVPVVLERNVSVGHLVMLHGCTIGENSLIGIGAIV
                     LNNARVGKNCLIGAGATVTEGKVIPDGSLVLGVNKIVRSLTPEEIATNTWIAEHYVER
                     AAAYRKGLKPLK"
     gene            657..1700
                     /locus_tag="VF309_01665"
     CDS             657..1700
                     /locus_tag="VF309_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX7465319.1"
                     /translation="MTPSRYRGSLAVWALLIAYASLYPFVPLRLPSMDAVLGYFSRPR
                     YVTAFDVAVNVLAYAPLGTLACLYFRQADATARAILKAIACCAAFSFAMEFLQLFVVG
                     RVASMYDTLANAAGALWGALVFADPFYSLVTRPLGERREQLIVPGAWGDAGLALVMLW
                     MIAQLNPALPFFGAGNIVGAGADPGDLVALQWAAVAASIWGFGLFVSSLLRGEHGALR
                     TTLVLLSVALWLKFVAATFMLQPHFAEEWLSVGRVLGLIAGIAMLVPSRRFGRAVRIY
                     LALMLVLAGALFSKIVGSYSPLDDFLRLFRWPYGQLASFATLTRFIHEAWPFMALVFL
                     IALFLRVRREAMP"
     gene            1710..2018
                     /locus_tag="VF309_01670"
     CDS             1710..2018
                     /locus_tag="VF309_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX7465320.1"
                     /translation="MSYYKHHVFFCLNKRDAPEACCACHDAEGMREYAKKRVKELKLS
                     GPGQVRINASGCLDRCEEGPVIVVYPEEVWYTYVDKSDIDEIIDEHLVKGRVVERLRI
                     "
     gene            2019..>2422
                     /locus_tag="VF309_01675"
     CDS             2019..>2422
                     /locus_tag="VF309_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7465321.1"
                     /translation="MPAATGRVLLEGASGKIEVVSSLPEAPRSIAVIAHPHPLFGGTM
                     DNKVVTTLARAFFEGGAATFRFNFRGVGASGGAHDEGRGETDDMLRVIAHAREFAGAL
                     PLQLAGFSFGGAVATRASATADFAQLVLVAPG"
BASE COUNT          351 a          820 c          802 g          449 t
ORIGIN      
        1 gtcgtctcgc gcatctactc ccacctgttc aaacagatgt gaagaaaatt ggtgtcaggt
       61 acgaatttcg gatgcatcgg ctcgccgatg ctctccgccg aaattcgtac ctgacaccaa
      121 ttttccgccg ataatatttc catgatctac cagctcggcg accgcaggcc caccttccac
      181 ggcgattact tcgtggccga caacgccgcg gtgatcggct cggtggtgat gggcgccaac
      241 gcgagcgtct ggtggaacgt gacgatccgc ggcgacaacg atctcatcac gctgggcgag
      301 aacgtgaacg tgcaggacgg ctccgtggtg cataccgaca gtggcgtccc ggtcgtgctc
      361 gaaaggaacg tgagcgtggg gcacctggtg atgctgcacg gctgcaccat cggcgagaat
      421 tcgctgatcg gcatcggcgc gatcgtgctc aacaacgcgc gggtcgggaa gaactgcctc
      481 atcggcgcgg gcgcgacggt gaccgaaggc aaggtgattc ccgacggctc gctggtgctc
      541 ggcgtgaaca agatcgtgcg ctcgctcacc cccgaggaga tcgcgaccaa cacctggatc
      601 gccgagcact acgtcgagcg tgccgcggca taccgcaagg gcctgaagcc gttgaaatga
      661 cgcccagccg ctaccgcggc tcgctcgcgg tctgggcgct gctcatcgcc tatgcgagcc
      721 tctacccgtt cgtgccgctg cggctgccgt cgatggatgc ggtgctcgga tacttctcgc
      781 ggcctcgcta tgtgacggcc ttcgacgtgg ccgtcaacgt gctcgcgtac gcgcccctcg
      841 gcacgctcgc ctgcctctac tttcgccagg ccgacgccac ggcgcgcgcc atcctcaagg
      901 ccatcgcctg ctgcgcggcg ttcagcttcg cgatggaatt cctgcagctc ttcgtggtgg
      961 gccgcgtcgc ctcgatgtac gacacgctcg ccaacgccgc gggcgcgctg tggggggcgc
     1021 tcgtcttcgc ggatccgttc tattcgctcg tcacgcgccc gttgggcgag cggcgcgagc
     1081 agctcatcgt gcccggcgca tggggcgacg cgggcctggc gctcgtcatg ctgtggatga
     1141 tcgcgcagct caatcccgcg ctgccgttct tcggcgcggg caacatcgtg ggcgccggcg
     1201 ccgacccggg cgacctcgtc gcgctgcaat gggccgcggt cgcggcgagc atctggggat
     1261 tcggcctctt cgtatcgtcg ctgctgcgcg gcgagcacgg cgccttgcgc acgacgctgg
     1321 tgctcctgag cgtcgccctc tggctcaagt tcgtcgccgc gaccttcatg ctgcagccgc
     1381 acttcgccga ggagtggctc agcgtgggac gcgtcctggg gttgatcgcc ggcatcgcga
     1441 tgctcgttcc gtcgcgccgc ttcggccgcg ccgtgcgcat ctacctcgcg ttgatgctgg
     1501 tgctcgccgg ggcgctcttc tccaagatcg tcggctccta cagcccgctc gacgatttcc
     1561 tgcgcctctt ccgctggccc tacgggcagc tcgcgagctt cgcgaccctc acgcgcttca
     1621 ttcacgaggc gtggccgttc atggcgctgg tgttcctgat cgcgctcttc ctgcgggtgc
     1681 gccgcgaggc catgccataa tcgcggccga tgagctacta caagcaccac gttttcttct
     1741 gcctgaacaa gcgcgatgcg cccgaggcgt gctgcgcctg ccacgacgcc gagggcatgc
     1801 gcgaatacgc caagaagcgc gtgaaggagc tgaagctgag cggcccggga caggtgcgca
     1861 tcaacgcctc gggctgcctc gaccgctgcg aggaaggccc cgtcatcgtg gtctatcccg
     1921 aggaggtctg gtacacgtac gtcgacaagt cggacatcga cgagatcatc gacgagcacc
     1981 tcgtgaaggg ccgcgtcgtc gagcgcctga ggatctagat gcccgccgcg accgggcgcg
     2041 tgctgctcga gggcgcgtcg gggaagatcg aggtcgtatc ttcgcttccc gaggctccgc
     2101 gctcgatcgc cgtgatcgcg catccccatc cgctcttcgg cgggaccatg gacaacaagg
     2161 tcgtgacgac gctcgcccgc gcattcttcg agggaggtgc ggcgacgttt cgcttcaact
     2221 ttcgcggcgt gggcgcgtcc ggcggcgccc atgacgaggg ccgcggcgag accgacgaca
     2281 tgctgcgcgt gatcgcgcac gcccgggaat tcgcaggcgc gttgccgttg cagctcgcgg
     2341 gcttttcctt cggcggcgcg gtggcgacgc gcgcgagcgc caccgcggat ttcgcgcagc
     2401 tcgttctggt ggcgcccggc tt
//
LOCUS       DASVII010000130         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_473863,
            whole genome shotgun sequence.
ACCESSION   DASVII010000130 DASVII010000000
VERSION     DASVII010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473863"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VF309_01680"
     CDS             complement(<1..936)
                     /locus_tag="VF309_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465322.1"
                     /translation="MRDTKQPLDLSPLNTDYDIIGELGATGVGRSFFGTRKDAAAKRR
                     DDNTGVLIEVLLPPEGDEGNALSHMASDAQTLMRTSHRRLVPVIEGRWIGKDVLAVVT
                     RRTTEPTLAQLLATGERFTNPRAAAILRDVNGLLEWAREQKIVHRGLTPHRIYLEPKT
                     DRVRATFEVSPIPRAQRDDAADVDARTIGRLAMAMLTGSMDPKAYEGRTLEELRPDLP
                     EQLIEETDALLTGKATNVDVAAYLALVGMADPLAAGESEAERVRAEVLEEQRVEREKL
                     ANERAEFERLMEEERQKLAREGEELRQKLAAEGEEL"
     gene            complement(1062..1529)
                     /locus_tag="VF309_01685"
     CDS             complement(1062..1529)
                     /locus_tag="VF309_01685"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEX7465323.1"
                     /translation="MLRIVLAVLHLLALGIGLGAIYSRARALQRVGASALALQRAFAA
                     DSWWGAAAALWLITGLWRAIAGTEKSPGYYLTNHIFYAKMGLLVLVLLLEIWPMATLI
                     RWRIAASRRTLPEGVVLASTGRRLARVSDVQTLLIVAMVVAAVLMARGYGAAG"
BASE COUNT          256 a          563 c          553 g          296 t
ORIGIN      
        1 cagctcttca ccctcggcgg ccagcttctg ccggagctct tccccttccc tcgccagctt
       61 ctggcgttct tcctccatca accgctcgaa ctcggcgcgt tcgttggcga gcttctcgcg
      121 ctccacgcgc tgctcctcca gcacctcggc gcgaacgcgc tcggcctccg attcgccggc
      181 ggcgagagga tcggccattc cgacgagcgc cagatatgcc gcgacgtcca cgttggtggc
      241 cttgccggtg agcagggcgt ccgtctcctc gatgagctgt tccggaagat cggggcgcag
      301 ctcttcgagc gtcctcccct cgtatgcttt cggatccatg cttcccgtga gcatcgccat
      361 ggcgagccgt ccaatcgtgc gcgcgtcgac gtcagcggca tcgtcgcgtt gagcgcgtgg
      421 aatgggcgat acctcgaagg tcgcgcgcac gcgatccgtc ttcggctcga gatagatgcg
      481 atgcggcgtg agaccgcgat gcacgatctt ttgctcgcgc gcccactcga gcagtccgtt
      541 gacgtcgcgg agaatggcgg cggcacgcgg attggtgaag cgctcgccgg tggcgaggag
      601 ctgtgcgagt gtgggctccg tggtgcgtct cgtcaccacg gcgagcacgt ccttgccgat
      661 ccaccgcccc tcgatcaccg gcaccaaccg ccgatgcgac gtccgcatca gcgtttgcgc
      721 gtcggacgcc atgtgcgaga gcgcatttcc ctcgtcgccc tccggcggaa ggaggacctc
      781 gatgagcacc ccggtgttgt catcgcgccg ttttgccgcg gcatccttcc tggtcccgaa
      841 aaagctgcgc cccacccccg tcgcccccaa ttcgccgatg atgtcgtaat cggtgttgag
      901 aggagagagg tcgagcggct gcttcgtatc gcgcatggag tgctccgtaa ccatcaatcg
      961 agaagggcgt gccgtgccgg cttcctattg cgcgaatgat gccgcgggct cagccggcca
     1021 gaggcggact catgcagcgg gccggagcac ctcgccgctc ttcaacccgc ggcgccgtac
     1081 cctcgcgcca tcagcaccgc ggccacgacc atggcgacga tcaggagcgt ctgcacgtcg
     1141 ctcacacgtg caagccggcg gccggtggag gcgagcacca ctccctccgg cagggtgcgc
     1201 cggctggcgg cgattctcca tcggatcagg gtggccatcg gccagatctc gagcaggaga
     1261 acgaggacga ggagtcccat cttggcgtag aagatgtgat tggtcaggta gtagccgggc
     1321 gatttttccg tgccggcaat tgcgcgccag agcccggtga tcagccagag cgccgccgcc
     1381 gcgccccacc aggagtcggc ggcgaatgcg cgctgcaagg cgagggcgga ggcgcccaca
     1441 cgctgtagcg cccgcgcgcg cgaatagatg gcgccgaggc cgatgccgag cgcgaggagg
     1501 tggaggactg cgaggacgat tcgtagcatg gttatccgcc gctcaccgcg ggtacgatgg
     1561 tgatggtgtc gccgtcgtcg acggcggtgg ccaggccgtt catgaagcga atgctctcct
     1621 ctcccacgaa gacgttgatg tggcgccgga gctcgccctg ctccgtca
//
LOCUS       DASVII010000131         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_617532,
            whole genome shotgun sequence.
ACCESSION   DASVII010000131 DASVII010000000
VERSION     DASVII010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617532"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF309_01690"
     CDS             complement(<1..317)
                     /locus_tag="VF309_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465324.1"
                     /translation="MAAPPQSPVGEPADLGFGTVVGGAKQRRLVNRDGTFTSRRVGFG
                     ALSYLNGYHALLTIGWPRFLAIVSVSYAAVNALFAGAFLMCGSDALRGLAPREMGGEF
                     LRA"
     gene            457..>1720
                     /locus_tag="VF309_01695"
     CDS             457..>1720
                     /locus_tag="VF309_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7465325.1"
                     /translation="MDPLLDRATLPEATRSRPRFIITALGIGYTVAYLLWAQFGGASA
                     SARAVVSESVLLPLTVTLILLFRAAAHVPGMGAGARRALALLSTFGVALLAGNLIGLY
                     YLLRDGRVPSGSIADALYLGGCVLVFASLLSVTGGRRTSSRWKLLCDTGMMLSGAGVA
                     LWFFAVRVSAAEAVNGRALLMAFMYPLGDLLLLVGTVNLIFRRSAEGHRRALTWLGLG
                     TAIGMVADLAFNLRIARGGVRASLVIDALYLAHYIAFLASVEFFLLGPVPETEGRIMR
                     AVGRARASLPWAAAGATYLLLLVTAVRNWSVPLSGVAVGAIAVSVFLGARQVLAFRRN
                     AELMAEAAVRASEARFRSLVQNSSDLILLLDEQGVVQFASSSAARLIGYEADALVGVD
                     SGALAHADDAALVSGYFDLARRFPGVLPA"
BASE COUNT          234 a          564 c          590 g          332 t
ORIGIN      
        1 aatgcgcgca ggaactctcc acccatctca cgtggcgcga gcccgcgcaa cgcgtccgaa
       61 ccacacatca gaaaggcgcc ggcaaacagc gcgttcacgg ccgcgtacga cacgctgacg
      121 atcgcgagaa agcgcggcca cccgatagtg agcagcgcgt gatacccgtt gagatacgac
      181 aacgcaccaa atcccacgcg gcgcgacgtg aaggtgccgt cgcggttgac caggcggcgt
      241 tgcttcgcgc cgccgacgac ggtgccgaag ccgagatccg ccggctcgcc gactggcgat
      301 tggggcggcg ctgccatgac gccctgcgaa ccgctctgat ttgttacagt gctctcagtc
      361 atcttatgag aaggttaaac aacggcgcgc ttgcgtcaaa cgcgcgtgtt gcgccacctt
      421 cataggtccg gattgtcgcc caccggccca ttcatgatgg atcctctcct cgaccgggcg
      481 acgctgcccg aagccacgcg ttcgcgcccg cgtttcatca tcacggcgct tggcatcggc
      541 tacaccgttg cgtacctgct ctgggcgcaa ttcggtggtg cctcggcgtc agcgcgtgcg
      601 gtggtcagcg aaagtgtgct gctgccgctc accgtcacgc tcatcctgct ctttcgcgcc
      661 gcggctcatg tgcccggcat gggcgccggc gcgcggcggg cgctggcgct gctgtcgacg
      721 ttcggtgtcg cgctgctcgc gggtaatctg atcggcctgt actacctgct ccgcgacggc
      781 cgcgtgccga gcggatccat cgccgacgcc ttgtatcttg gcggctgcgt gctggtgttc
      841 gccagccttc tctcggtaac cggcggacgc cggacgtcga gccgatggaa gcttctgtgc
      901 gacacgggga tgatgctctc cggcgccggc gtggcgctct ggttcttcgc ggtgcgtgtg
      961 agcgccgcgg aagcggtgaa tgggcgcgcg ctcctgatgg cgttcatgta tccgcttgga
     1021 gatctcctcc tgctcgtggg cacggtgaat ctgatctttc ggcggtccgc cgaagggcac
     1081 cgccgagccc tgacctggct tgggctaggc acggcgatcg gcatggtggc ggatctcgca
     1141 ttcaatctgc gaatcgcgcg cggcggcgtg cgggcttcac tggtcatcga tgcgctgtac
     1201 ctggcgcact acatcgcctt tcttgcgagt gtggagttct tcctgctcgg gcctgtaccg
     1261 gagacggagg ggcggatcat gcgtgcggtc ggccgcgcac gggcatctct cccatgggca
     1321 gccgccggcg cgacgtatct actgttgctc gtgacggccg ttcggaactg gtcggtgcca
     1381 ctcagcggcg tggccgtggg cgccattgcg gtgtccgtct ttctcggcgc gcggcaggtg
     1441 ctggcgttcc ggcggaatgc ggagctcatg gcggaggcgg ccgttcgcgc cagcgaagcg
     1501 cgctttcgct cgctggtcca gaactcgtcc gacctgatcc tgctcctcga tgagcagggg
     1561 gtggtgcagt tcgccagctc gtcggcggcg cgcctgatcg gctatgaggc ggacgcgctc
     1621 gttggcgtgg acagcggagc gctcgcgcac gccgacgacg cggcgctcgt gagcggctac
     1681 ttcgatctcg cgcgacgctt tcccggtgtg ctgccggcgg
//
LOCUS       DASVII010000132         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_689569,
            whole genome shotgun sequence.
ACCESSION   DASVII010000132 DASVII010000000
VERSION     DASVII010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689569"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..742
                     /locus_tag="VF309_01700"
                     /pseudo
     CDS             44..742
                     /locus_tag="VF309_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
     gene            776..1393
                     /locus_tag="VF309_01705"
     CDS             776..1393
                     /locus_tag="VF309_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX7465326.1"
                     /translation="MTRLAARELRDGFYVNLGIGIPTLVANYIPAGMTVHLQSENGLL
                     GIGPFPAEDQVDPDLINAGKQTITTLPGSSFFSSADSFGMIRGGHVDLSILGGLEVAE
                     NGDLANWMVPGKMVKGPGGAMDLVSGVKRVVVLMEHTSKDGASKVLKRCTLPLTGKGV
                     VNKIITDLCVFEVEPGEGLVLTELHPGVTLEDVKAKTAADFRVAL"
     gene            complement(1594..>1775)
                     /locus_tag="VF309_01710"
     CDS             complement(1594..>1775)
                     /locus_tag="VF309_01710"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX7465327.1"
                     /translation="VLKRCTLPLTGKGVVNKIITDLCVFEVEPGEGLALTELHPGVAL
                     EDVKAKTAADFRVAL"
BASE COUNT          343 a          569 c          571 g          292 t
ORIGIN      
        1 tcgtttgccg ctacaattcc cccatccatt tttggggaac gcaatgagca aagtctatcc
       61 ggacgccaag tccgccctgc aaggcctgct gaaggacgac atcaccatcg ccgccggcgg
      121 cttcgggctt tgcggcattc acgagaacct gatcgccgcg ctggtggaaa gcggcgtgaa
      181 ggggctcacc atctgcggca acaacgcggg cgtggacgac ttcggcatgg ggctcttact
      241 gaagacgcac caggtgaaga aggtgatcgc ctcctacgtg ggcgagaaca aggaattcga
      301 acgccaggtg ctggcgggcg agctcgaatt gcagctcacg ccgcagggca cgctcgcgga
      361 gaaattccgc gccggcggcg ccggcatccc gggcttctac acgcgaaccg cgtacggcac
      421 gcgcctcgcc gagaagcacg acacccatgt cttcgacggc cgcgagtacg tgctcgagga
      481 ggcgatccac gccgatgtgt ccatcgtcaa ggcgtggaag ggcgacaagc tcggcaacct
      541 cgtctatcgc aagacctcgc gcaacttcaa ccccatgatc gccacctgcg gcaaggtgac
      601 ggtggccgag gtcgaggagc tcgtggaggt gggcgagctc gaccccgaca acatccacac
      661 gccgggcatc tacgtcgacc gcatcatcca gggcgcgaag tacgaaaagc gcatcgagtt
      721 tcgcaccgtg tcgggcgggg cggcctcgaa gaaggattcg ccgatccgcg atcttatgac
      781 gcggctcgcc gccagggaat tgcgcgacgg cttctatgtg aacctcggca tcggcattcc
      841 gacgctcgtc gccaactaca ttccggccgg catgaccgtg cacctgcagt cggaaaacgg
      901 gctcctcggc atcgggccct tcccggccga ggaccaggtc gaccccgatc tcatcaacgc
      961 gggcaagcag accatcacca cgctgcccgg atcgagcttc ttctccagcg ccgattcctt
     1021 cggcatgatc cgcggcggcc acgtggacct gtcgattctc ggcggtctcg aagtcgccga
     1081 gaacggcgac ctcgccaact ggatggtgcc gggcaagatg gtgaagggcc cgggcggcgc
     1141 gatggacctg gtgtccggcg tgaagcgcgt ggtggtgctg atggagcaca cctcgaagga
     1201 cggcgcgtcg aaggtcctca agcgatgcac gctgccgttg accggcaagg gcgtggtcaa
     1261 caagatcatc acggaccttt gcgtattcga ggtggagccg ggcgagggac tggtgctgac
     1321 ggagctgcat ccgggcgtga cgctcgagga tgtgaaggct aagacggccg cggatttccg
     1381 cgtggcgctg tagctctggc ggagttggct atcgcctcat tgaagtgttg gggtgaagct
     1441 cgagaggcgc gcgtcaccgg cggtcaatcg agagaagacg ccaacagttc gagcggaatg
     1501 ggttgcagcg agatccctcc cctcccaagg aacgatcgcg ccggctcgag cttcaccccc
     1561 acttcaatga ggcgatagcc aactccgctc gagctacaaa gccacgcgga aatccgcggc
     1621 cgtcttggcc ttcacatcct cgagcgcgac gcccggatgc agctccgtca gcgccagccc
     1681 ctcgcccggc tccacctcga agacgcacag gtccgtgatg atcttgttga ccacgccctt
     1741 gccggtcaac ggcagcgtgc atcgcttgag gacct
//
LOCUS       DASVII010000133         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_42509, whole
            genome shotgun sequence.
ACCESSION   DASVII010000133 DASVII010000000
VERSION     DASVII010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42509"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1079
                     /locus_tag="VF309_01715"
     CDS             189..1079
                     /locus_tag="VF309_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7465328.1"
                     /translation="MKVVVPIKDVLSNPYQARKTMNREAIRNLAEEIKASGLWPGSLR
                     GRMKGSHVELCYGHRRLAALKHLGWDKVEVEVDELTDEEMALQGLAENFQREGLSDIE
                     KAQGLHMMVQLLIKQKVSESDAMKRVSKFVGLSEAWIRDLLSMLDMEGPVQRAIRDRK
                     IAGRTALEAHRFGGKQMVATAVIHKLPVHKISAIAKRVRSIPDADVRDSIRKDVLRGR
                     IVEPEKVEEKARKMLKGRKIKAPEDLDRVLADWTYLVKHWNEKIDEVLIYRRFFADRN
                     VASLQSEASKLSKKLQKLVE"
     gene            complement(1146..>1698)
                     /locus_tag="VF309_01720"
     CDS             complement(1146..>1698)
                     /locus_tag="VF309_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465329.1"
                     /translation="LDAYIAEGQAQGLHQNYKCRTRKPWYSVRRQLPPPDLLLGYLVK
                     RRPRLADNPGGVHTTNNLHRIYLKKPWVGSSALLAAASLNAATMLTVELLGRIGAAGV
                     LKIEPGDASKIRLVPPEALGAVRGAAESVTAIDRALREGRDDDAFAIADDLASRALRW
                     NPREMSKLRRAQVALRDARLKPA"
BASE COUNT          309 a          553 c          563 g          273 t
ORIGIN      
        1 ccgatttcct ttcggcgggg tcgttcgttt cacggcaaag tcgagtgaac cggttttttt
       61 cctgcgtttt ctcggcgatg acgccgctcg cgcgctgcgg ctcgattgat tgacagctcg
      121 agcgggtcgt gatagaacca acacgtcatc gcacgcacga acagctgggg cataggagga
      181 ggggtcgtat gaaagtggtc gttcccatca aggacgttct gtccaatccg taccaggccc
      241 gcaagaccat gaaccgcgag gccatccgca acctcgcgga agagatcaaa gcctccggcc
      301 tctggcccgg ctcgctccgc ggccgcatga aaggctcgca cgtcgagctc tgctacggcc
      361 accggcgcct cgccgccctc aagcacctcg gctgggacaa ggtcgaggtc gaggtcgacg
      421 agctcaccga cgaggagatg gccctgcagg gcctcgccga gaacttccag cgcgaagggc
      481 tgagcgacat cgagaaggcg cagggtcttc acatgatggt ccagctcctc atcaagcaga
      541 aggtcagcga aagcgacgcg atgaagcgcg tctcgaaatt cgtcggcctc tccgaggcgt
      601 ggatccgcga tctgctgtcc atgctcgaca tggaaggccc ggtccagcgg gcgatccgcg
      661 accgcaagat cgccggtcgc accgcgctgg aggcccaccg cttcggcggc aagcagatgg
      721 tcgccaccgc cgtcattcac aagctccccg tccacaagat cagcgcgatc gccaagaggg
      781 tccggtcgat ccccgacgcc gacgtgcgcg actcgatccg caaggacgtc ctgcgcggac
      841 gcatcgtcga gccggagaaa gtcgaggaga aggcgcggaa gatgctcaag ggccgcaaga
      901 tcaaggcgcc cgaggatctc gaccgcgtgc tggccgactg gacctatctg gtcaagcact
      961 ggaacgagaa gatcgacgag gtcctcatct acaggagatt cttcgccgac cgcaacgtcg
     1021 cctcgctgca gtccgaagcg tcgaagctct cgaagaagct gcagaagctg gtggaatagc
     1081 ggatccccgg aactcttcaa acccgcaagg atgcgaaagc cccttgcggg ttttttgttt
     1141 tccggtcagg ccggcttgag ccgcgcgtcg cggagcgcga cctgcgcgcg gcgcagcttc
     1201 gacatctcgc ggggattcca gcggagcgcg cgggaggcca ggtcgtccgc gatcgcgaac
     1261 gcgtcgtcgt cgcgtccctc gcggagcgcg cggtcgatcg ccgtcacgct ttccgccgcg
     1321 cccctcaccg cgccgagggc ctcggggggc accaggcgga tcttggacgc gtcgcccggc
     1381 tcgatcttca gcacgcccgc ggcgccgatg cggccgagca gctcgaccgt cagcatcgtc
     1441 gcggcattca aggaggcggc ggcgagcagc gccgaagagc cgacccaggg cttcttcagg
     1501 tagatgcgat gcaggttgtt cgtggtatgg acgccgcccg ggttgtcggc gagacgcgga
     1561 cgccgtttca ccagataccc gagcagcagg tcgggcggcg gaagctggcg ccgcacggag
     1621 taccagggct tccgggtgcg gcacttgtag ttctggtgca gcccctgcgc ctggccctcg
     1681 gcgatatagg cgtccagc
//
LOCUS       DASVII010000134         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_258124,
            whole genome shotgun sequence.
ACCESSION   DASVII010000134 DASVII010000000
VERSION     DASVII010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258124"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VF309_01725"
     CDS             <1..1146
                     /locus_tag="VF309_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465330.1"
                     /translation="QTRADLDAWFKGGATRRALPARLREFRTTKAAPRAMRAAAAPDR
                     DALAKEILASIERDTKFQGALRSAQAGLHPQRQALRMARGGGTRVSTTSLIDKEAAAR
                     LFPPVRRRTTQLSVGGWIQAAKVVADIVLRVLRRVRTGRDHGKYVTLVEEVLRELYVG
                     KIGRIGWWDRMKDDTAATFRTGEQYGGSVFLQALHDALGGAAAPRITLVGHSTGAIFI
                     GNFLKAAAQWVPDVMFDVIFEAPAATHDFLSKVIDEHGSRIRNLRLFGMSDAREQADV
                     LVPLIYPASLLYFVSGLLEDEPDQPIVGMQRFLANASTFDPDGFPNVDACRKFFGRFN
                     DALVWSPQLKPPGAASDGRHHQDFDDVDPPTLQSVAFILRNGYATNP"
     gene            1208..2011
                     /locus_tag="VF309_01730"
     CDS             1208..2011
                     /locus_tag="VF309_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465331.1"
                     /translation="MRELSGVAAALQKQATRDGAPIWQLETTVTAFEKLEDLPLDYWP
                     VIVKDDIGDPQAAGYHEDKNGQPFALVEYSKGWTLTASHEVVEMLVDPFGRRLVAGQS
                     PVQKQGRVRFLVEVCDPSEAEAYAYRVNGIQVSDFYTPHFFDPMKSAGVRYSFTNAIT
                     EPKQVLKGGYLSWYDSETKEWWQRSWFGAKASDEALTGFKATNGNLREALDRYRELER
                     AKVMGRVEESTGKRTAMARRGGFQDNDMLQAPGRAAMLRETIAQVSRRR"
BASE COUNT          372 a          698 c          735 g          318 t
ORIGIN      
        1 cagacccgcg ccgatctcga cgcctggttc aagggcgggg ccacgcgcag ggcgctgccg
       61 gcgcggttgc gcgagttcag gacgacgaag gcggcgcccc gcgcgatgcg cgccgccgcc
      121 gcacccgatc gcgacgcgct cgcgaaggag atcctcgcga gcatcgagcg cgacacgaag
      181 ttccagggcg cgctgcgctc ggcgcaggcg gggcttcatc cgcaacgcca ggcgctgcgc
      241 atggcgcgcg ggggcggcac gcgcgtgtcg accacctcgc tcatcgacaa ggaggccgcg
      301 gcgcgcctct ttccgccggt gcgccgcagg accacgcagc tttccgtagg aggatggatc
      361 caggcggcga aggtcgtggc cgacatcgtg cttcgggtgt tgcgccgggt ccgcacgggc
      421 cgcgaccacg ggaagtacgt gacgctcgtc gaggaggtgc tgcgcgagct ctacgtcgga
      481 aagatcggcc gcatcggctg gtgggaccgg atgaaggacg acaccgcggc tacgttcagg
      541 acgggcgagc aatacggcgg cagcgtcttc ctgcaggcgc tgcacgacgc gctcggcggc
      601 gcggccgcgc cgcgcatcac gctcgtcggc cacagcacgg gtgcgatctt catcggcaat
      661 ttcctcaagg ccgccgcgca gtgggttccc gacgtgatgt tcgacgtgat cttcgaggcg
      721 cccgcggcga cccacgactt tctctcgaag gtgatcgacg agcacgggag ccgcatccgc
      781 aacctgcggc tcttcggcat gagcgatgcg cgcgagcagg ccgatgtcct cgtgccgttg
      841 atctacccgg catcgctcct ctatttcgtg tcgggccttc tcgaggacga gcccgaccag
      901 ccgatcgtcg gcatgcaacg cttcctcgcg aacgcctcga ccttcgatcc cgacggcttc
      961 ccgaacgtgg acgcctgccg caagttcttc ggccgcttca acgacgcgct cgtgtggtcg
     1021 ccgcaattga agccgccggg cgccgcgtcg gacggcaggc atcaccagga tttcgacgac
     1081 gtcgatcccc cgacgctgca gagcgtcgca ttcatcctgc gcaacggtta cgccaccaac
     1141 ccctgaggga ggaagcgcca tgttgatgcg tcacgtagca ttggtttcgg aagaatcgaa
     1201 gatcacgatg cgcgagctga gcggggtcgc cgccgcgctg cagaagcagg cgacgcgcga
     1261 cggcgcgccg atctggcagc tcgagaccac ggtgacggcc ttcgagaagc tcgaggacct
     1321 gccgctcgac tattggccgg tcatcgtcaa ggacgacatc ggcgatccgc aggccgcggg
     1381 ctaccacgag gacaagaacg ggcagccctt cgccctcgtg gagtattcga aggggtggac
     1441 cctgacggcg agccacgaag tggtggagat gctggtggat cccttcggtc gccgcctcgt
     1501 cgcgggccag tcgccggtgc agaagcaggg gcgcgtgcga tttctcgtcg aggtctgcga
     1561 tcccagcgag gcggaggcct atgcctatcg cgtgaacggc atccaggtga gcgatttcta
     1621 cacgccgcac tttttcgacc cgatgaaatc ggcgggcgtt cgctacagct tcaccaacgc
     1681 gatcaccgag cccaagcagg tgctgaaggg cggctacctc agctggtacg actcggagac
     1741 gaaggagtgg tggcagcgca gctggttcgg cgccaaggcg agcgacgagg cgctgacggg
     1801 attcaaggcg accaacggca acctgcgcga agccctcgat cgctaccgcg agctcgagcg
     1861 cgcgaaggta atggggcgcg tcgaggaatc cacgggcaag cgcaccgcga tggcgcgacg
     1921 cggcggcttc caggacaacg atatgttgca agcgcccggg cgagcggcca tgctgcgtga
     1981 aaccattgcg caggtatcgc gtcgaaggta ggtcatgccg aagaaaccga aggcccccgc
     2041 ttttcccgag ccctcacccg cgcagaagga gcgggcggcg ggccttcgaa gcctcatcaa
     2101 ggacatcgcc tcgggcgtga agc
//
LOCUS       DASVII010000135         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_473917,
            whole genome shotgun sequence.
ACCESSION   DASVII010000135 DASVII010000000
VERSION     DASVII010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473917"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF309_01735"
     CDS             complement(<1..269)
                     /locus_tag="VF309_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465332.1"
                     /translation="MNFKLKTLAAAAAMAFAAAGAQAQISGNVVKIGVLNDQSGLYAD
                     LGGQGSVTAAKMAIEDFKAKEKGLNVEVIFADHQNKADVAGAKAR"
     gene            complement(298..1005)
                     /locus_tag="VF309_01740"
     CDS             complement(298..1005)
                     /locus_tag="VF309_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465333.1"
                     /translation="MADEVLRISGLQAWYGESHVLHGVDLAVNKGEVVTLLGRNGAGR
                     TSTLKSILGLVGRRTGSIVVNGVETIDWPTYRIAHLGIGYCPEERGIFSSLSVEENLM
                     LPPAIGANGMSVQEIYEMFPNLGERRNTSQGTKLSGGEQQMLAMARILRTGANLLLLD
                     EITEGLAPVIVQALGRIIIKLKERGFTIVLVEQNFRFAAPLADRHYVVEHGRIAESFP
                     ASELEAKTAMLHEYLGV"
     gene            complement(998..1774)
                     /locus_tag="VF309_01745"
     CDS             complement(998..1774)
                     /locus_tag="VF309_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465334.1"
                     /translation="MGALLPSMTGDTILETRGLTKEFRGFVAVDHVSLAVRRGAIHAL
                     IGPNGAGKTTTFNLLTKFLQPTAGTILFNGLDITRERPADVARRGIIRSFQISAVFPN
                     LTVLENVRIALQRAVGTSFHFWKREKTLEVLNGRAMELLEQVGLEGFARVNAGELAYG
                     RKRTLEIATTLAMEPELMLLDEPTAGMGHEDVDRVKDLIKEVAVGRTVLMVEHNMSVV
                     SGICDTITVLQRGTVLAEGSYAQVSGNPQVMEAYMGTAHG"
     gene            complement(1940..>2123)
                     /locus_tag="VF309_01750"
     CDS             complement(1940..>2123)
                     /locus_tag="VF309_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465335.1"
                     /translation="PIQGIPNPTPLVIGCTPSCGAGATGSFDGFFAGRTGGRAGMMYN
                     LGGNQGAIAFGRPGGP"
BASE COUNT          338 a          705 c          699 g          381 t
ORIGIN      
        1 tcgcgcgcct tggcgccggc gacgtcggct ttgttctggt ggtcggcgaa gatcacctcg
       61 acattgaggc ccttctcctt cgccttgaag tcctcgatcg ccatcttggc ggccgtgacc
      121 gagccctgcc cgccgagatc ggcgtaaagc cccgactggt cgttcagcac gccgatcttc
      181 accacgttgc ccgagatctg ggcttgggcg cccgcggcgg cgaacgccat cgccgcggcg
      241 gcagccaatg tcttcagctt gaaattcatg ctcgttgctc ctctagtttt cgatgaacta
      301 cacacccagg tactcgtgaa gcattgccgt cttcgcttcg agttccgacg ccgggaacga
      361 ctcggcgatc ctgccgtgct ccacgacgta atgccggtcc gcgagcggtg ccgcgaaccg
      421 gaaattctgt tccaccagga cgatggtgaa gccgcgctcc ttcagcttga tgatgatgcg
      481 cccgagagcc tggacgatca ccggcgcgag gccctcggtg atctcgtcga gcaggagcag
      541 gttcgcgccc gttcgcagga tgcgcgccat ggcgagcatc tgctgctcgc cgcccgagag
      601 cttggtgccc tgggaagtgt tgcggcgctc gcccaggttg ggaaacatct cgtagatctc
      661 ctgcacgctc atgccgttcg cgccaatcgc gggcgggagc atgaggttct cctcgaccga
      721 gaggctcgag aagatgccgc gctcctcggg gcagtagccg atgccgaggt gcgcgatcct
      781 gtaggtgggc cagtcgatcg tctccacgcc gttcacgacg atggagcccg tcctgcgccc
      841 gacgaggccc aggatcgact tgagcgtcga agtgcgcccg gcgccgttgc gccccaggag
      901 cgtgacgacc tcgcccttgt tcaccgcgag atccacgccg tgcagcacgt gggactcgcc
      961 gtaccaggcc tgcaggcccg agatgcgcag gacttcgtca gccatgggcc gttcccatat
     1021 aggcttccat cacctgcgga ttgcccgaga cttgcgcata ggagccctcg gcgaggaccg
     1081 tgccccgctg cagcaccgtg atcgtgtcgc agatgccgga gacgacgctc atgttgtgct
     1141 cgaccatgag caccgtgcgc ccgacggcga cttccttgat gaggtccttc acgcgatcga
     1201 cgtcctcgtg gcccatcccc gcggtcggct cgtcgaggag catgagctcg ggctccatcg
     1261 cgagcgtggt cgcgatctcg agcgtgcgct tgcgcccgta ggcgagctcg cccgcgttga
     1321 cccgcgcgaa gccctcgagc cccacctgct cgaggagctc catcgcgcgg ccgttcagca
     1381 cttcaagcgt cttctcccgc ttccagaaat gaaagctcgt gccgacggcg cgctgcagcg
     1441 cgatgcgcac gttttcgagc accgtgaggt tcgggaacac cgccgatatc tggaacgagc
     1501 ggatgatgcc gcggcgggcg acgtcggcgg gccgctcgcg cgtgatgtcg aggccgttga
     1561 aaagaatcgt ccccgccgtg ggctgcagga acttggtgag caggttgaaa gtggtcgtct
     1621 tgccggcgcc gttgggcccg atgagcgcgt ggatcgcgcc gcggcgcacc gcgaggctca
     1681 catggtcgac cgcgacgaag ccccggaatt ccttggtgag gcctcgcgtt tcgagaatgg
     1741 tgtcgccggt catcgagggc aggagcgcgc ccaacgtcac cgcgacgcgg tgcgtgggct
     1801 gctatggaaa ttgtccgccg atgctacccg gcgcggccga catgggcaag gagtcggaag
     1861 aagaaatctc ccgattaatt cgccatttac ctccgaagtg tccggcaatc cggacacccc
     1921 gcgaggaagt cttcggcgtt cagggtcccc cggggcggcc gaaggcgatg gccccctggt
     1981 tgccgccaag gttgtacatc attcccgcgc ggccgccggt gcggccggcg aagaagccgt
     2041 cgaagctccc cgtcgcgccg gcgccgcaac tgggcgtgca gccgatgacg agcggcgtgg
     2101 gattcgggat accctgaatc ggc
//
LOCUS       DASVII010000136         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_437950,
            whole genome shotgun sequence.
ACCESSION   DASVII010000136 DASVII010000000
VERSION     DASVII010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437950"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="VF309_01755"
     CDS             complement(<1..1577)
                     /locus_tag="VF309_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7465336.1"
                     /translation="MIEATYEVGRPTVFSMIIIIAAHIPIFTLQRHEGRIFSPMAWTV
                     TSALVGSLIFSLTLVPLLCAKLLKKDLAHGDNRLVEWIKSHYEPLLRKTICRPGLITG
                     IAVGALVAAFALASQLGSEFLPELNEGTTWVNLTLPPSVSPAEAQEQLRAVRAALHTV
                     PEVNTVISKAGRPDDGMDPKIFNSAEIFVDFVPESKWRPGKGKDDFIREMDTAVSAIP
                     GMEPSFSQPIRDNVLESISQVDGQIVIKLRGDDLDQINADAAQVLEHISGVPGVVRAF
                     IDRAGSLPQYIIDIDRLAASRYGINVGDIQDLVETALAGKATSELWEGEKHFSVVVRL
                     KPAERDLGDLPKLLVASPNGAQVPLSQLARFRSVSGAMDIARENGRRVVSIGVFIRDR
                     DMGSVVKDMKERVKSDVKLAPGEEVSWSGEFENQERAMKRLSWIVPLSIALIFVLLFD
                     AFGSFRSAFLIIANIPFAMIGGILALYLLGIPLSVSAAIGFIALFGQAVLNGVVMVTY
                     FNQLQRAGATPAEAVIKG"
BASE COUNT          268 a          524 c          543 g          252 t
ORIGIN      
        1 cccttgatca cggcttcggc cggcgttgcc ccggcgcgct ggagctggtt gaaataggtc
       61 accatcacga cgccgttcag caccgcctgg ccgaagagcg cgatgaagcc gatcgccgcc
      121 gacaccgaga gcggaatgcc cagcaggtac agcgcgagga tccctccgat catcgcgaac
      181 gggatgttgg cgatgatgag gaacgcgctc ctgaaggatc cgaacgcgtc gaagaggagc
      241 acgaagatga gcgcgatcga cagcggcacg atccacgaca ggcgcttcat cgcgcgctcc
      301 tggttctcga actctcccga ccacgacacc tcttcgccgg gcgcgagctt cacgtcgctc
      361 ttcacgcgct ccttcatgtc cttcacgacg ctgcccatgt cgcggtcgcg gatgaagacg
      421 ccgatggaga ccacgcgccg cccgttctcg cgggcgatgt ccatcgcgcc cgacaccgac
      481 cggaagcgcg cgagctgcga cagcggcacc tgtgcgccgt tgggcgaggc gacgaggagc
      541 ttcggcaggt cgcccaggtc gcgctccgcg ggcttgaggc gcaccacgac gctgaaatgc
      601 ttctcgcctt cccagagctc ggacgtcgcc ttgccggcga gcgccgtctc gacgaggtcc
      661 tggatgtcgc ccacgttgat gccgtagcgc gacgccgcca ggcgatcgat gtcgatgatg
      721 tactgcggaa gggagcccgc gcggtcgatg aacgcgcgca ccacgccggg cacgcccgag
      781 atgtgctcga ggacctgggc cgcgtcggca ttgatctggt cgaggtcgtc gccgcgcagc
      841 ttgatcacga tctgcccgtc cacctgcgag atgctctcga gcacgttgtc gcggatcggc
      901 tgcgagaacg agggctccat gcccgggatc gcggagaccg cggtatccat ctcgcggatg
      961 aagtcgtcct tccccttccc cgggcgccac ttcgattcgg gaacgaagtc gacgaagatc
     1021 tcggcggagt tgaagatctt cgggtccatg ccgtcgtcgg ggcggcccgc cttcgagatc
     1081 accgtgttca cctcgggaac cgtgtgcagc gccgcgcgca cggcgcgcag ctgctcctgc
     1141 gcctcggcgg gcgacaccga cgggggcagc gtgaggttca cccaggtcgt gccttcgttc
     1201 agctccggaa ggaactcgct gccgagctgc gaggcgaggg cgaaggccgc cacgagggcg
     1261 ccgaccgcga ttcccgtgat cagcccgggc ctgcagatgg tcttgcgcag cagcggctcg
     1321 tagtgcgact tgatccactc gacgaggcgg ttgtcgccgt gcgcgagatc cttcttgagg
     1381 agcttcgcgc agaggagcgg cacgagcgtg agggagaaga tgagcgagcc caccagcgcg
     1441 gaggtcacgg tccaggccat cggcgagaag atgcggccct cgtggcgctg cagcgtgaag
     1501 atcgggatgt gcgccgcgat gatgatgatc atcgagaaga ccgtcggccg gccgacctcg
     1561 taggtggcct cgatcaccgc gccgtag
//
LOCUS       DASVII010000137         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_186263,
            whole genome shotgun sequence.
ACCESSION   DASVII010000137 DASVII010000000
VERSION     DASVII010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186263"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1058)
                     /locus_tag="VF309_01760"
     CDS             complement(117..1058)
                     /locus_tag="VF309_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465337.1"
                     /translation="MLSFLLKRLAAIIPTLFFVSVLIFGLQQLLPGDPAIAMAGEERD
                     PNVIAYLRGKFHLDDPLPVRYWYWVSGVFHGDLGDSVRIQKPVVELIGEKLPVTIELA
                     VLAMTIALTIGISAGIVSAVLKDTKWDYAANIFALWGLSTPNFWLGILMILLFAVKLG
                     WLPASGYVSPFEDLKANLAAMIMPAFVLGNAIAAVLMRHTRSAMLQVLSSDYVRTARA
                     KGLDERVVVLRHALRNALIPIVTLGALQFGELLGGAVLTEQVFSIPGFGKLIVDAVFN
                     RDYSVVQGVVLFTATAYITLNLLADIAYFAINPRMRA"
     gene            complement(1059..>2179)
                     /locus_tag="VF309_01765"
     CDS             complement(1059..>2179)
                     /locus_tag="VF309_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465338.1"
                     /translation="VVDPATVRLNLSAPFAPLLAILTDRAGMMVSPKAAQSAGEKFGA
                     KPVCSGPFKFAERVAQDRIVLERFPNYWNKGAIHFDKVVYQPITDSTVLLANLKSGQL
                     DMIERMAPSDVPALQSDKRFKVAKVVEIGYQGITFNIGKSELSQKNPAGKDPRVREAF
                     ELSLDRDAIVQVAMSGEALPGNQWVAPTNSWYAKNVPIPKRDLARAKALLKEAGVPNA
                     SFKLMTPTTSDAQRVAQVVQAMAREAGFDVKIESTEFSTTLDLADKGQYEAFVLAWSG
                     RPDPDGNLFSHLACKQPLDYSGNCRADMDDLINKSRATVDPAARLKLFEQIAAMEAKD
                     RPIVYLYHRHWLWAYTTRLSGFRTVPDGMIRLQDLKLN"
BASE COUNT          379 a          702 c          740 g          358 t
ORIGIN      
        1 gaccagcgcg aggccgacca tcgcgccgcg gcggcgcacg aggcgcttca gcgcgcgctg
       61 cgccggcgtg agctctgcgc gcgccgcggg aatgggaatc gcgatcgccg ccacgctcat
      121 gccctcatgc gcggattgat cgcgaagtac gcgatgtcgg cgagcaggtt cagggtgatg
      181 taggccgtcg cggtgaagag caccacgccc tgcacgacgg agtagtcgcg gttgaagacc
      241 gcatcgacga tgagcttgcc gaacccggga atggagaaca cctgctcggt cagcaccgcg
      301 ccgccgagga gctcgccgaa ctgcagggcg ccgagcgtca cgatcgggat caacgcgttg
      361 cgaagcgcgt ggcgcagcac caccacgcgc tcgtcgagac ccttggcgcg cgccgtgcgc
      421 acgtagtccg acgacagcac ctgcagcatg gcgctgcgcg tgtggcgcat cagcaccgcg
      481 gcgatcgcgt tgccgagcac gaaggcgggc atgatcatcg cggcgaggtt cgccttgagg
      541 tcctcgaagg ggctcacgta tcccgacgcg ggcagccacc cgagcttcac cgcgaagagc
      601 aggatcatga ggatgccgag ccagaagttg ggcgtggaaa gcccccacag cgcgaagatg
      661 ttcgccgcgt aatcccactt cgtgtccttc agcaccgccg agacgattcc cgccgagatt
      721 ccgatggtga gcgcgatcgt catcgcgagg accgcgagct cgatggtgac gggaagcttc
      781 tcgccgatca gctcgaccac cggcttctgg atgcgcaccg aatcgccgag gtcgccgtgg
      841 aagacgccgc tcacccagta ccagtagcga accggcagcg gatcatccaa atggaacttg
      901 ccgcgcaggt acgcgatgac gttcggatcg cgctcctcgc ccgccatcgc gatcgccgga
      961 tcgccgggca gcagctgctg caggccgaag atcaggaccg acacgaagaa gagcgtcggg
     1021 atgatcgccg cgaggcgctt gaggaggaaa ctgagcattc agttcagctt caggtcctgc
     1081 agccggatca tcccgtcggg caccgtgcgg aatccggaaa gcctggtcgt gtaggcccac
     1141 agccagtgcc gatggtagag gtagacgatg ggccggtcct tcgcctccat cgccgcgatc
     1201 tgctcgaaga gcttcaggcg cgccgcggga tccaccgtgg cgcgcgactt gttgatgaga
     1261 tcgtccatgt cggcgcggca gttgcccgag taatcgagcg gctgcttgca agcgaggtgg
     1321 ctgaagagat tgccgtcggg gtccgggcgc ccgctccacg cgagcacgaa cgcctcgtac
     1381 tgtcccttgt cggcgagatc gagcgtagtc gagaactcgg tggactcgat cttcacgtcg
     1441 aagcccgcct cccgcgccat cgcctgcacc acctgcgcga cgcgttgcgc atccgacgtg
     1501 gtcggcgtca tgagcttgaa gctcgcgttg gggaccccgg cctctttcag cagcgccttc
     1561 gcgcgcgcca gatcgcgctt cgggatcggg acgttcttcg cgtaccagga gttcgtgggc
     1621 gcgacccatt ggtttcccgg aagcgcttcg ccgctcatcg cgacctgcac gatcgcgtcg
     1681 cggtcgaggg acagctcgaa ggcctcgcgc acgcgcggat ccttcccggc gggattcttc
     1741 tgcgagagct cgctcttgcc gatgttgaag gtgatgccct ggtagccgat ctcgacgacc
     1801 ttcgcgacct tgaagcgctt gtcggactgc aatgccggca cgtcggaggg cgccatgcgc
     1861 tcgatcatgt cgagctggcc gctcttcagg ttggcaagga gcaccgtaga atcggtgatc
     1921 ggctggtaga ccaccttgtc gaagtggatc gcgcccttgt tccagtagtt cgggaagcgc
     1981 tcgaggacga tgcggtcctg cgccacccgc tcggcgaact tgaacggtcc cgagcacacg
     2041 ggcttcgcgc cgaatttttc acctgcggac tgcgccgcct tgggcgacac catcatgccg
     2101 gcgcgatcgg tgaggatcgc gaggagcggc gcgaagggcg cggagagatt gaggcgcacc
     2161 gtggccgggt cgacgacgt
//
LOCUS       DASVII010000138         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_474126,
            whole genome shotgun sequence.
ACCESSION   DASVII010000138 DASVII010000000
VERSION     DASVII010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474126"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VF309_01770"
     CDS             <1..572
                     /locus_tag="VF309_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7465339.1"
                     /translation="IRMPEMDGLEAARHIAGMADPPAIIFTTAFDSYAIKAFEVNAID
                     YLLKPIRVERLLVALGKTRAAPPVSRQALDAAANQPRRHLSVHERGKIILVPVADILY
                     LRAELKYVTVKTVEREHLVEESLTHLEEEFAESFVRIHRNCLVARAAIAGFERNAEES
                     ESGWAVVIKGTNEKLPVSRRQHGVVKQFR"
     gene            complement(596..1498)
                     /locus_tag="VF309_01775"
     CDS             complement(596..1498)
                     /locus_tag="VF309_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7465340.1"
                     /translation="MDRFKELTTFVEVAQRGSLSAAAREEGITPAMVGRRIDQLEERL
                     GVKLFKRSTRKVTLTPEGSTFYEDCHRILVELRSAEDSLTVGAKNASGRLIVTAPTAF
                     GRKHIAPHLSAFIAEHPNLAITLHLSERLVDLKNERFDLAIRIADLKSADLIAAKLAR
                     NHRVVCGSPSYFKRAGKPRVLADLAKHNCLVTSTEDGVADTWSFLDKGKTVAVKVGGN
                     LQCNDGEVLTRWAIAGEGLAWRSAWEVSEEVKRARLVTVLDDFAFPGNNIYAVYPERR
                     LLPAKVKFFIEFLRNTFGDPPYWE"
     gene            complement(1671..2090)
                     /locus_tag="VF309_01780"
     CDS             complement(1671..2090)
                     /locus_tag="VF309_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7465341.1"
                     /translation="MTALNRPMLATLLLLATGLCSAEIFRCTARDGGVTYQQDPCAAS
                     SNGGAVSIPTSYPDYSDERERLARREAALDARLLKRLEIEANERIARDDRIAREKEAQ
                     AALELAQAQQAYPVYGIARPLQVPRRLPHRPLPAGVH"
     gene            complement(2141..3088)
                     /gene="lipA"
                     /locus_tag="VF309_01785"
     CDS             complement(2141..3088)
                     /gene="lipA"
                     /locus_tag="VF309_01785"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7465342.1"
                     /translation="MTDPDLKLQQKRGLKTSRIPIKVVQSEPLRKPEWIRVRAGGGER
                     FTEIKKILREANLHTVCEEASCPNIGECFGHGTATFMIMGDLCTRRCPFCDVAHGRPQ
                     PLDRDEPRHLGETIAKMGLTYVVITSVDRDDLRDGGAEHFVECIRAVRSLSPLTKIEI
                     LTPDFRGRLDRALAILGASPPDVMNHNLETVPRLYRQARPGSDYAHSLQLLRDFKARH
                     PRVPTKSGLMVGLGETPGEILEVMRDLRAHDVDMLTIGQYLQPSRHHLPVARFAHPDE
                     FRMFGEEAARMGFVNAACGPMVRSSYHADRQAKEAGATG"
     gene            complement(3081..3710)
                     /gene="lipB"
                     /locus_tag="VF309_01790"
     CDS             complement(3081..3710)
                     /gene="lipB"
                     /locus_tag="VF309_01790"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7465343.1"
                     /translation="MKARNLGLVAYQPAWRAMQAFNQARDAQTPDELWLVEHPPVFTL
                     GLAGRREHVLDAGGIPVVATDRGGQVTYHGPGQAIAYVLLDLRRNGLGVKELVRRIEG
                     AVIDTLGEYGIDGARRAGMPGVYVNEAKIAAIGLRIARGCSYHGVALNVDADLAPFAR
                     IDPCGYPGLASARLADLGVRDSINAVQQRLAASLSRCLAPRPAKQLHDD"
     gene            complement(3731..4012)
                     /locus_tag="VF309_01795"
     CDS             complement(3731..4012)
                     /locus_tag="VF309_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEX7465344.1"
                     /translation="MSLPEPAGNESLLTFPCVFPIKVMGRREDGFAQTVCDIVGKHAA
                     DFHPGTIEMRASKNGRYLSLTVTLNARSRGQLDALYAELSKHPMVMMVL"
     gene            complement(4018..>4256)
                     /locus_tag="VF309_01800"
     CDS             complement(4018..>4256)
                     /locus_tag="VF309_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEX7465345.1"
                     /translation="ARLAADGALKLEIRPVSEAQLRNADEVWLSSSTKEVLAVTTLDG
                     KPVGTGRPGPVFRRMHSLYQDYKSKLRKARPVAA"
BASE COUNT          670 a         1412 c         1444 g          730 t
ORIGIN      
        1 acatccgcat gcccgagatg gacggcctgg aggccgcgcg ccacatcgcg ggcatggccg
       61 acccgcccgc gatcatcttc acgaccgcct tcgactccta cgcgatcaag gccttcgagg
      121 tgaacgccat cgactacctc ctgaagccga tccgggtgga gcgactgctg gtcgcgctcg
      181 gcaagacgcg cgcggcgccg ccggtgtcgc gccaggccct cgatgcggcg gccaaccagc
      241 cgcgccgcca cctctcggtc cacgagcgcg gcaagatcat cctcgtcccg gtcgccgaca
      301 ttctctatct cagggccgag ctcaagtacg tgaccgtgaa gaccgtcgag cgcgagcacc
      361 tggtcgagga gtccctcacg cacctcgagg aggagttcgc cgagtccttc gtgcgcatcc
      421 accgcaactg cctcgtcgcg cgcgccgcga tcgcgggctt cgagcgcaac gccgaggaaa
      481 gcgaatcggg atgggccgtg gtgatcaagg gcaccaacga gaagcttccc gtcagccggc
      541 gacagcacgg ggtggtgaag caattccggt gaaaaatggt ctctgacacc agttttcact
      601 cccagtaggg agggtcgccg aaagtattgc gaaggaactc gatgaagaac ttgaccttcg
      661 ccggcaggag gcgccgctcg gggtacaccg catagatgtt gttgcccgga aacgcgaagt
      721 cgtcgaggac ggtcaccagg cgagcgcgtt tcacctcctc gctcacttcc caggcgcttc
      781 gccacgcgag gccctcgccg gcgatcgccc atcgggtcag cacctcgccg tcgttgcact
      841 gcaggttgcc gccgaccttg accgcgacgg tctttccctt gtcgaggaag ctccatgtgt
      901 cggcgacacc gtcctcggtg ctcgtcacga ggcagttgtg cttcgcgagg tcggcgagga
      961 cccggggctt gccggcgcgc ttgaagtagg agggcgagcc gcacacgacg cgatggttgc
     1021 gcgcgagctt cgcggcgatc aggtcggcgc tcttcaggtc ggcgatgcgg atcgcgaggt
     1081 cgaagcgctc gttcttcagg tcgacaaggc gctccgacag gtgcagcgtg atcgcgaggt
     1141 tggggtgctc cgcgatgaac gccgaaaggt gcggcgcgat gtgcttgcgc ccgaaggccg
     1201 tcggggcggt cacgatgagc cgtccggagg cgttcttcgc gcccaccgta agcgagtctt
     1261 ccgcggaacg cagctcgacg aggatgcgat ggcaatcctc gtagaacgtc gagccttcgg
     1321 gcgtaagcgt gaccttgcgc gtcgagcgct tgaagagctt gacgccgagg cgctcttcga
     1381 gctggtcgat gcgtcgcccg accattgccg gcgtgatgcc ttcctcgcgc gccgccgccg
     1441 ataggctgcc gcgctgggcc acctcgacga aggtggtgag ctccttgaat cggtccattt
     1501 cccctcgaaa gtatcaaccg cttcgctgtt ttttgttatt gatggtatgc gagttgggta
     1561 atgaactgca agctccggcg cgaaaggcgc cttcgattca tcggcgcgat gcccgcgcgg
     1621 cgccgcgcga agaagggcgc gccgccgcgg cgatcgatcg atttcgcggc ttagtgaacg
     1681 ccggccggca atgggcgatg cggaagccgg cgcggcactt gcagcggacg cgcgatcccg
     1741 tagaccgggt aagcctgttg ggcctgggca agctcgagcg cggcctgcgc ttccttctcc
     1801 cgcgcgatgc ggtcgtcgcg cgcgatgcgc tcgttggctt cgatctcgag gcgcttgaga
     1861 agccgcgcat cgagcgccgc ctcgcggcgc gcaagacgct cgcgctcgtc ggagtaatcg
     1921 ggatagctcg tcgggatgct cacggcgccg ccgttgctcg acgcggcgca cggatcctgc
     1981 tggtaggtaa cgccgccgtc gcgcgccgtg cagcggaaga tctccgccga gcacaggccc
     2041 gttgcgagga gcagcagcgt tgcgagcatc ggtcgattca gggcggtcat ggtctgccct
     2101 ccttgcgcga acaccaagat actccgggga attcccggcg tcatcctgtc gcgcccgcct
     2161 ctttcgcctg gcggtcggcg tggtagctcg atcgcaccat cggcccgcag gccgcgttca
     2221 cgaagcccat cctggccgcc tcctccccga acatcctgaa ctcgtccggg tgcgcgaagc
     2281 gcgccacggg aaggtggtgc ctcgacggct gcaggtattg gccgatggtg agcatgtcga
     2341 cgtcgtgcgc tcgcaggtcg cgcatgacct cgaggatctc cccgggcgtc tcgcccaatc
     2401 cgaccatgag ccccgacttg gtcggcacgc gaggatgacg cgccttgaag tcgcgcagca
     2461 gctgcagcga atgcgcgtaa tccgagcccg gccgcgcctg gcggtagagg cgcgggaccg
     2521 tctcgaggtt gtggttcatg acatcgggcg gcgaggcgcc gagaatggcg agcgcgcgat
     2581 cgaggcggcc ccggaagtcg ggcgtcagga tctcgatctt cgtgagcggc gagaggctcc
     2641 ggaccgcgcg gatgcattcg acgaaatgct ccgcgccgcc atcgcgaaga tcgtcgcggt
     2701 cgacgctcgt gatcacgacg taggtgaggc ccatcttcgc gatggtctcg ccgaggtggc
     2761 gcggctcgtc gcgatcgagg ggctggggcc ggccgtgggc cacgtcgcag aaggggcagc
     2821 gccgcgtgca aagatcgccc atgatcatga acgtggccgt gccgtggccg aagcactcgc
     2881 cgatgttcgg gcacgaggct tcctcgcaaa cggtgtgcag gttcgcctcg cgcaggatct
     2941 tcttgatctc ggtgaagcgt tcgccgccgc cggcgcgcac gcgaatccac tcgggcttgc
     3001 gcaagggctc ggattgcacg accttgatcg ggatgcggga ggtcttgagg ccgcgcttct
     3061 gctggagctt gagatccgga tcagtcatcg tggagttgtt tcgcaggtcg cggcgcgagg
     3121 caccgcgaga gcgatgcggc aaggcgttgc tgcactgcat taatgctatc acgcacgccg
     3181 aggtcggcga ggcgcgccga ggcgaggccc ggatagccgc acgggtcgat gcgcgcgaag
     3241 ggcgcgaggt cggcgtcgac gttcagcgcg acgccgtgat agctgcaccc gcgcgcgatg
     3301 cgaaggccga tcgcggcaat cttcgcctcg ttcacgtaga cgccgggcat tcccgctcgc
     3361 cgcgcgccgt cgatgccgta ctctccgagg gtatcgatca ccgcgccctc gatccttcgc
     3421 accagctcct tcacgccaag gccgtttctc cgcaggtcga gaagcacgta ggcgatcgcc
     3481 tgtcccggcc cgtgataggt gacctgcccg ccgcgatcgg tcgcgaccac cggtatcccg
     3541 cccgcgtcga gcacgtgctc gcgccggccc gcgagcccca gcgtgaacac cggcggatgc
     3601 tcgacgagcc acagctcgtc cggcgtttgc gcgtccctcg cctggttgaa cgcctgcatc
     3661 gcccgccacg cgggctggta cgcgaccagc cccaggttgc gtgccttcat cgcgccgcgc
     3721 ctcgcttccc tcacaggacc atcatgacca tcggatgctt cgacagctcc gcgtacaggg
     3781 cgtcgagctg cccgcgcgag cgcgcgttga gcgtcaccgt gagcgagaga taccggccgt
     3841 tcttcgaggc tcgcatctcg atcgttccgg gatggaaatc cgcggcgtgc ttccccacga
     3901 tgtcgcatac ggtttgcgcg aaaccatcct cgcgccttcc catcaccttg atgggaaaca
     3961 cgcaggggaa cgtcagcagc gattcgttcc cagccggttc cgggaggctc atcggcatca
     4021 cgctgcgacg ggacgggcct ttcgcagctt ggacttgtag tcctggtaga gcgaatgcat
     4081 gcggcggaat accggcccgg gcctgccggt tccgaccggc ttgccgtcga gcgtggtcac
     4141 cgcgagaacc tccttcgtcg acgaggagag ccagacttcg tcggcgttgc gcaattgcgc
     4201 ttcgctgacg ggccggattt cgagcttcag ggcgccgtcc gcggccaggc gcgcga
//
LOCUS       DASVII010000139         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_114735,
            whole genome shotgun sequence.
ACCESSION   DASVII010000139 DASVII010000000
VERSION     DASVII010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114735"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1817)
                     /gene="kefC"
                     /locus_tag="VF309_01805"
     CDS             complement(39..1817)
                     /gene="kefC"
                     /locus_tag="VF309_01805"
                     /inference="COORDINATES: protein motif:HMM:NF002924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HEX7465346.1"
                     /translation="MHEALIFLAAAVICVPIAARLGLGSVLGYLIAGVAIGPWGFAFI
                     GNVDSTQNLAELGVVLMLFVIGLELEPKRLMSMRSTVFGGGTLQLAVSGAALTGALMA
                     LGLVWQAALAAGLALALSSTAISTQAMTERGELDTPTGRAAFGILLFQDIAAIPLIAL
                     VPLLARVETPGGNPLWMRVGLAVAAIAGVVVLGRYFIRPALRLIARIDIRELFTAFAL
                     LLVIGVAELMSLAGLSMAMGAFLAGVLLASSEFRHALESDIEPFKGLLLGLFFISVGM
                     TIDFGLLASRPATVLLLVVGFVALKYASLWAVARMLDICRERWLFAALLAQGGEFAFV
                     VFGVARESKLFSAEWEALLTITVALSMALTPLLLLLHDRLAARATKTERAADTIDAQA
                     PVIIAGFGRFGQIVGRLLLANDIRAVVLDHDPDQVESLRKFGYRVFYGDATRLDLLEA
                     AGARRARLLVNAIDDVESSIALVDRVRANFPDLPIVSRARNVSHYFELKLRGVEVAER
                     ETFEAALRVGRSALERLGIDRFRARELADAFRRHNIASVDATLPFYKDEARRMSIAKQ
                     GREELEQQFARDRERFEREHGGAGWK"
     gene            complement(1810..2382)
                     /gene="kefF"
                     /locus_tag="VF309_01810"
     CDS             complement(1810..2382)
                     /gene="kefF"
                     /locus_tag="VF309_01810"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HEX7465347.1"
                     /translation="MAVAAAVAAVILVILAHPYPRRSRACAALVEAIRDLPEVEIRSL
                     YDLYPDFDVDPAAEQAALARARLVVWLHPLFWYAAPGLMKHWFDEVLVRGFAYGPGGD
                     ALAGKDVLWATTTGGDEWAFSEAGRHHRPFDAFVPVIEQTARFCGLNWLAPFVVHGAH
                     EIADEALRDAGRRLRERLVAWRATMEARNA"
     gene            complement(2352..3242)
                     /gene="ubiA"
                     /locus_tag="VF309_01815"
     CDS             complement(2352..3242)
                     /gene="ubiA"
                     /locus_tag="VF309_01815"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX7465348.1"
                     /translation="MNKVLARIDAYEKLMRLHRPIGILLLLWPTLWALWLAGHGVPKP
                     LVLLLFVVGTVLMRSAGCALNDYADRAFDAQVERTRDRPLAAGLIQPWEALAVAVACA
                     LLAFAIVLRFNELTVKLSFAALAVAAVYPFLKRFFWMPQAWLGIAFGFGIPMAYAALL
                     DSIPGVAWALLAANIFWTIAYDTEYAMVDRDDDARIGIRTSAILFGKHDVAAVMGCYA
                     LFIATMAAIGAWQRYGPFYFAGLGLALSICLAHYGWIRGRAREGCFRAFLGNNWVGAA
                     VFLGIVLDNRPWQSLQRWLP"
     gene            complement(3239..>4364)
                     /locus_tag="VF309_01820"
     CDS             complement(3239..>4364)
                     /locus_tag="VF309_01820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX7465349.1"
                     /translation="WQAALMAPTEILAEQHYLKLSHWLEPLGVRIAWLAGSLKARDRK
                     KAVEAIASGETQLAIGTHALFQEGVEFAKLGLAIVDEQHRFGVEQRLALAAKSKARTE
                     PHQLMMSATPIPRTLSMSYYADLDVTVIDELPPGRTPVVTKLVSEEGRDEVISRIRES
                     CREGAQAYWVCPLIEESEALQLQTAIETFETMKSTFPDLAVGLVHGRLKPVEKAQVMA
                     DFKARKIQLLVATTVIEVGVDVPNATLMVIEHAERMGLAQLHQLRGRVGRGSKQSLAI
                     LLYYQPLSELARQRLKVIYESTDGFEIAREDLRLRGPGEYLGARQSGAPLLRFADLER
                     DIDLIEAARAAAEDLLRAKSPAVVKHLDRWLGSRQFYLRA"
BASE COUNT          719 a         1512 c         1547 g          586 t
ORIGIN      
        1 gacgcttctt cccgcgcctc gcgcctcgtg cctcgccgct atttccatcc cgcgcctccg
       61 tgctcccgtt cgaaccgctc gcggtcgcgg gcgaactgct gctcgagctc ctcgcgcccc
      121 tgcttggcga tcgacatgcg ccgcgcctcg tccttgtaga acggcagggt cgcatcgacg
      181 ctcgcgatat tgtgccgccg gaacgcatcg gcaagctcgc gcgcgcggaa ccggtcgatg
      241 cccagccttt ccagcgccga gcgtcccacc cgcaacgccg cctcgaaggt ctcgcgctcg
      301 gcgacctcga cgccgcgcag cttcagctcg aagtagtgcg acacgttgcg cgcgcggctc
      361 acgatcggca ggtccgggaa gttggcgcgg acacggtcga ccagggcgat gctcgactcg
      421 acgtcgtcga tcgcgttcac caggagccgc gcccggcgcg cgcccgccgc ctctagcaga
      481 tcgaggcgcg tcgcgtcgcc gtagaacacc cggtagccga acttgcgcag cgactccacc
      541 tggtcgggat cgtggtcgag caccaccgca cggatgtcgt tggcgaggag gaggcgcccc
      601 acgatttgcc cgaagcggcc gaagcccgcg atgatgaccg gcgcctgcgc gtcgatggtg
      661 tccgccgcgc gctcggtctt cgtagcgcgc gccgcgaggc ggtcgtgcag cagcaggagc
      721 agcggcgtga gtgccatcga cagggcgacg gtgatggtga ggagggcctc ccactccgcg
      781 gagaagagct tcgactcgcg cgccacgccg aacacgacga aggcgaactc gccgccctgc
      841 gcgaggagcg cggcgaagag ccaccgctcg cggcagatgt cgagcatgcg cgccaccgcc
      901 cagagcgagg cgtacttgag cgcgacgaag cccacgacga gcagcagcac cgtcgcggga
      961 cggctcgcga ggaggccgaa gtcgatggtc attcccaccg agatgaagaa gagcccgagg
     1021 agcaggccct tgaacggctc gatgtcgctc tcgagcgcgt ggcggaattc cgagctcgcg
     1081 aggagcacgc ctgcgaggaa cgcgcccatc gccatcgaaa ggccggcgag cgacatgagc
     1141 tccgccacgc cgatcacgag gagcagcgcg aacgccgtga agagctcgcg gatgtcgatg
     1201 cgcgcgatga gccgcagcgc cgggcggatg aagtagcggc ccaggaccac cacgcccgcg
     1261 atggccgcga ccgcgagccc gacgcgcatc cacagcggat tgcctcccgg cgtctcgacg
     1321 cgcgcgagga gcggcacgag cgcgatgagc ggaatggcgg cgatgtcctg gaagaggagg
     1381 atgccgaagg cggcgcggcc cgtcggcgtg tcgagctcgc cgcgctcggt catcgcctgg
     1441 gtcgagatcg ccgtggacga cagagccagc gcaagcccgg cggcgagggc ggcctgccag
     1501 acgagcccga gcgccatgag cgcgccggtc agcgccgcgc cgctcacggc gagctggagc
     1561 gtgccgccgc cgaatacggt ggagcgcatc gacatgagcc gtttcggctc gagctcgagg
     1621 ccgatcacga agagcatgag gaccacgccg agctcggcga ggttctgcgt cgagtcgacg
     1681 ttgccgatga aggcgaatcc ccacggcccg atggcgacac cggcaatgag gtagccgagg
     1741 acggagccca gcccgagccg cgccgcgatc ggcacgcaga tcacggccgc cgcgaggaag
     1801 atcaacgcct catgcattgc gcgcctccat cgttgcgcgc catgccacga ggcgctcgcg
     1861 caggcggcgc cccgcgtcgc gaagcgcctc gtcggcaatc tcgtgcgctc cgtgcacgac
     1921 gaagggcgcg agccagttga gcccgcagaa gcgcgccgtc tgctcgatca ccgggacgaa
     1981 cgcatcgaac gggcggtggt ggcggcccgc ttcgctgaac gcccactcgt cgccgcccgt
     2041 cgtcgtcgcc cacagcacgt ccttgccggc gagggcgtct ccgccggggc cgtaggcgaa
     2101 gccgcgcacc agcacctcgt cgaaccagtg cttcatgagg cccggggcgg cgtaccagaa
     2161 gagcggatgc agccacacca cgaggcgcgc gcgagcgagc gcggcctgct cggcggccgg
     2221 gtcgacgtcg aagtcgggat acaggtcgta gagcgagcgg atctcgacct cgggcaggtc
     2281 gcgaatcgct tcgaccagcg cggcgcacgc gcgcgagcgg cgcggatacg ggtgcgcgag
     2341 gatgacgagg atcacggcag ccaccgctgc agcgactgcc acggccggtt gtcgagaacg
     2401 atgccgagga agaccgcggc gccgacccag ttgttcccga gaaaggccct gaagcagccc
     2461 tctcgcgcgc gcccgcggat ccagccgtag tgcgcgaggc agatcgagag cgcgaggccg
     2521 agccccgcga agtagaaggg cccgtagcgc tgccacgcgc cgatcgcggc catcgtcgcg
     2581 atgaagagcg cgtaacaacc catcaccgcc gcgacgtcgt gctttccgaa gaggatcgcg
     2641 gacgtcctga tgccgatcct cgcgtcgtcg tcccggtcga ccatcgcgta ttccgtgtcg
     2701 taggcgatgg tccagaagat gttggccgcg aggagcgccc acgcgacgcc cggtatcgag
     2761 tccagcagcg cggcgtaggc catcgggatg ccgaacccga acgcgatccc gagccacgcc
     2821 tgcggcatcc agaagaagcg cttgaggaac gggtagaccg ccgcgacggc gagcgccgcg
     2881 aaggaaagct tgacggtgag ctcgttgaac ctcagcacga tcgcgaaggc caggagcgcg
     2941 cacgccacgg cgaccgcgag cgcttcccac ggctggatga gcccggcggc gaggggccga
     3001 tcgcgggtgc gctccacctg cgcgtcgaaa gcgcggtcgg cgtagtcgtt gagcgcgcac
     3061 cccgccgagc gcatgagcac ggtgcccacg acgaagagaa gcagcacgag gggtttcggc
     3121 acgccgtggc ccgcgagcca cagcgcccag agcgtcggcc acagcagcag caggatgccg
     3181 atgggccgat gcagccgcat cagcttctcg tacgcgtcga tgcgcgcgag gactttgttc
     3241 atgcccgcag gtagaactgg cgcgagccga gccagcggtc gaggtgcttc acgaccgccg
     3301 gcgacttcgc cctgaggaga tcctcggcgg cggcgcgcgc ggcctcgatg agatcgatgt
     3361 cgcgctcgag gtcggcgaag cgcagcagcg gcgcgccgct ctggcgcgcg ccgagatact
     3421 cgccggggcc gcgcaggcgc aggtcctcgc gggcgatctc gaagccgtcg gtggactcgt
     3481 agatcacctt gagccgctgg cgcgcgagct ccgagagcgg ctggtagtag aggaggatcg
     3541 cgaggctctg cttcgagccg cgcccgacgc gcccgcgcag ctggtgcagc tgcgcgaggc
     3601 ccatgcgctc ggcgtgctcg atcaccatga gcgtcgcgtt gggcacgtcg acgcccacct
     3661 cgatcaccgt cgtcgcgacg aggagctgga tcttgcgtgc cttgaagtcg gccatcacct
     3721 gcgctttctc gacgggcttc aggcgcccgt gcaccaggcc cacggcgagg tcggggaagg
     3781 tgctcttcat cgtctcgaag gtctcgatcg ccgtctgcag ctgaagagcc tccgattcct
     3841 cgatgagcgg gcacacccag taggcctgcg cgccctcgcg gcagctctcg cggatacggc
     3901 tgatcacttc gtcgcggccc tcctccgaca cgagcttcgt gaccacgggc gtgcgccccg
     3961 gcggcagctc gtcgatcact gtcacgtcaa ggtccgcgta gtagctcatg gagagcgtgc
     4021 gcgggatggg agtggcgctc atcatgagct ggtgcggctc ggtcctcgcc ttgctcttgg
     4081 cggcgagggc gagccgctgc tcgacgccga agcggtgctg ctcgtcgacg atcgcgaggc
     4141 ccagcttcgc gaactccacg ccctcctgga agagcgcatg ggtgccgatc gcgagctgcg
     4201 tctcgccgct tgcgatcgcc tccacggcct tcttgcggtc gcgcgccttg aggcttcccg
     4261 cgagccacgc gatcctgacg cccagcggct cgagccagtg ggagagcttg aggtaatgct
     4321 gctcggcgag gatctccgtg ggcgccatga gcgccgcctg ccag
//
LOCUS       DASVII010000140         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_617926,
            whole genome shotgun sequence.
ACCESSION   DASVII010000140 DASVII010000000
VERSION     DASVII010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617926"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VF309_01825"
     CDS             complement(<1..446)
                     /locus_tag="VF309_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX7465350.1"
                     /translation="MKNYPHPIIAREGWPYLAASLVAAILVTWLFGWWSIPFWIVYLF
                     ILQFFRDPPREIPQGANLVLCPADGRVLLVGNMRDPYRNVEALKVSVFMNVFNVHSNR
                     SPVDAAVKDVVYNKGLFVNASFDKASSDNERNALILRMDDGTELACV"
     gene            complement(469..1485)
                     /gene="ilvC"
                     /locus_tag="VF309_01830"
     CDS             complement(469..1485)
                     /gene="ilvC"
                     /locus_tag="VF309_01830"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX7465351.1"
                     /translation="MKVYYDKDADLSLIKGKKVTIIGYGSQGHAHAQNLTDSGVKVTV
                     GLRPGGASWDKVKKAKITVKEVGEAVKGADFVMILLPDENHAQVYKDSIEPNIKKGAT
                     LAFAHGFNIHFRQIMPRADLDVIMIAPKGPGHLVRQTYTQGGGVPALIAIHQDASGKA
                     KDLALSYAAAIGGTRGGVIETNFREETETDLFGEQTVLCGGIVELIKAGYETLVEAGY
                     APEMAYFECLHETKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPKVIGDEARKAMKE
                     CLARIQSGEYARDFILENRAGAPTLNARRRLLAEHDIEKVGEKLRAMMPWIKKNKLVD
                     TSRN"
     gene            complement(1512..2003)
                     /gene="ilvN"
                     /locus_tag="VF309_01835"
     CDS             complement(1512..2003)
                     /gene="ilvN"
                     /locus_tag="VF309_01835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX7465352.1"
                     /translation="MRHILSILVENEAGALSRVAGLFSARGYNIETLTVAPTEDLTMS
                     RMTIVTAGSDEVVEQITKQLNKLVDVVKVVDLSEGKHIERELMLVKVRAGGKDREEIK
                     RTADIFRGRIIDVTDKSYTIELTGTTEKLDAFLEALDRAAILETVRTGVSGIGRGEWV
                     LKV"
     gene            complement(2091..>2550)
                     /locus_tag="VF309_01840"
     CDS             complement(2091..>2550)
                     /locus_tag="VF309_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7465353.1"
                     /translation="GLGTMGVGLPYAMGVKLAFPDADVACVTGEASIQMCIQELSTCK
                     QYHLPVKIVNLNNRYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAEAYGHVGMKIEK
                     PADIEGALREAFAMKDRLVFLDFITDQTENVFPMVPGGKGISEMILSEEL"
BASE COUNT          410 a          820 c          795 g          525 t
ORIGIN      
        1 acgcaggcga gctccgtgcc gtcgtccatg cgcaggatca gggcgttgcg ctcgttgtcg
       61 gacgacgcct tgtcgaaaga ggcgttcacg aacaggccct tgttgtacac gacgtccttc
      121 accgccgcgt ccaccggcga acggttcgag tgcacgttga agacgttcat gaagacgctc
      181 accttcagcg cctcgacgtt gcgatacgga tcgcgcatgt tgcccaccag cagcacgcgg
      241 ccgtcggcgg ggcacagcac gaggttcgcg ccctgcggga tttcgcgggg cggatcgcgg
      301 aagaactgga ggatgaagag gtagacgatc cagaacggga tcgaccacca gccgaagagc
      361 caggtgacga gaatcgcggc aacgagagac gcggcgaggt agggccaccc ttccctcgcg
      421 atgatcggat gcgggtagtt tttcatctcg ccctctcgtg cctcgtgcct agttcctgct
      481 cgtgtccacc agcttgttct tcttgatcca cggcatcatg gcgcgcagct tctcgcccac
      541 cttctcgatg tcgtgctcgg cgagcaggcg ccgcctcgcg ttgagcgtcg gtgcgccggc
      601 gcggttctcc aggatgaagt cgcgcgcgta ctcgcccgac tggatgcgcg ccaggcactc
      661 cttcatcgcc ttcctcgcct cgtcgccgat caccttcggc ccggtcacgt actcgccgta
      721 ttcggcgttg ttcgagatgg agtagttcat gttcgcgatg ccgccctcgt agatcaggtc
      781 cacgatgagc ttggtctcgt gcaggcactc gaagtacgcc atctcgggag cgtaacccgc
      841 ctccaccagc gtttcgtatc ccgccttgat gagctcgacg atgccgccgc agagcacggt
      901 ctgctcgccg aagaggtcgg tctcggtttc ctcgcggaaa ttggtctcga tcacgccgcc
      961 gcgggtcccg ccgatcgccg ccgcgtagga aagggccagg tccttcgcct tgccggacgc
     1021 gtcctggtga atcgcgatga gcgcgggcac gcctccgccc tgcgtgtagg tctggcgcac
     1081 caggtggccg ggccccttcg gcgcgatcat gatcacgtcg agatcggcgc gcggcatgat
     1141 ctggcggaag tggatgttga agccgtgcgc gaaggcgagc gtagcgccct tcttgatgtt
     1201 gggctcgatg gaatccttgt agacctgcgc gtggttctcg tcgggcagca ggatcatcac
     1261 gaagtcggct cccttcaccg cctcgccgac ttccttcacc gtgatcttcg ccttcttcac
     1321 cttgtcccac gatgcgccgc cgggacgcag cccgacggtg accttcaccc ccgaatcggt
     1381 gaggttctgc gcatgcgcgt ggccctgcga gccgtagccg atgatcgtga ccttcttgcc
     1441 cttgatgagc gaaaggtccg cgtccttgtc gtagtacacc ttcatgttcg ttcccctgtt
     1501 tgcggtttgg gtcagacctt cagcacccac tcgccgcgcc cgatgccgga tacgccggtg
     1561 cggacggttt ccaggatcgc ggcccgatcg agcgcttcga ggaaggcgtc gagcttctcg
     1621 gtggtgccgg tgagctcgat cgtgtaggac ttgtcggtga cgtcgatgat gcgcccgcga
     1681 aagatgtccg cggtgcgctt gatctcctcg cggtccttgc cgccggcgcg gaccttcacg
     1741 agcatgagct cgcgctcgat gtgcttgccc tccgaaaggt cgaccacctt cacgacatcg
     1801 accagcttgt tcaactgctt cgtgatctgc tcgaccactt catcggagcc cgcggtgacg
     1861 atcgtcatgc gcgacatcgt gaggtcctcg gtgggtgcca ccgtgagcgt ctcgatgttg
     1921 tagccgcgag cggagaaaag cccggccacg cgcgacagcg cgccggcttc gttttcgacg
     1981 aggatggaaa ggatatgtct catggcgcgc agttactcga ggaaggcgcg aggcacgagg
     2041 cgcgagaagc ttcgcttgtt ctcgtgcctc ttgcctgcaa ccctaggttc ttatagttct
     2101 tcggaaagga tcatctccga gatgcccttg ccgccgggca ccatcgggaa gacgttctcg
     2161 gtctggtcgg tgatgaagtc gaggaagacg aggcggtcct tcatcgcgaa cgcctcccgc
     2221 agcgcgccct cgatgtcggc aggcttctcg atcttcattc ccacgtggcc gtaggcttcg
     2281 gcgagcttca cgaaatcggg cagcgcgtcc atgtacgact ccgcgtagcg gttgccgtgg
     2341 aagaactcct gccactggcg caccatcccc atgtagcgat tgttcaggtt cacgatcttc
     2401 accggcaggt gatattgctt gcaggtggaa agctcctgga tgcacatctg gatcgacgcc
     2461 tcgcccgtca cgcatgcgac gtcggcatcg ggaaacgcga gcttgacgcc catggcgtac
     2521 ggaagcccca cccccatggt cccgaggccg
//
LOCUS       DASVII010000141         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_546246,
            whole genome shotgun sequence.
ACCESSION   DASVII010000141 DASVII010000000
VERSION     DASVII010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546246"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..265
                     /locus_tag="VF309_01845"
     CDS             44..265
                     /locus_tag="VF309_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465354.1"
                     /translation="MTNKTLFSIAALAGFVSYCFYRGGRTAHAVRREAKHDLHRWEGE
                     GGNVPQVATVSPVPVPPGPRPGNGAAASH"
     gene            complement(305..1702)
                     /gene="fumC"
                     /locus_tag="VF309_01850"
     CDS             complement(305..1702)
                     /gene="fumC"
                     /locus_tag="VF309_01850"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX7465355.1"
                     /translation="MSGMRIEKDTFGPIEVPDERLWGAQTQRSLEHFRISGERMPAEI
                     IQALAWVKRACATVNHSLGGLTAEKAGAISQAADEVLAGKWPDEFPLVVWQTGSGTQT
                     NMNVNEVLANRASEMLGGARGEGRLVHPNDDVNRGQSSNDVFPTAMHVAAVVSIRRRL
                     LPALETLRATLAAKSEQFARIVKIGRTHLQDATPLTLGQEFSGYAAQLDHSRRHVESA
                     LPHLSELALGGTAVGTGLNAPPRFGELAAAEISRLTGCEFVTAPNKFEALATHDAMVH
                     AHGSLKGLAASLMKIANDVRLLASGPRSGIGEITIPENEPGSSIMPGKVNPTQCEALT
                     MCACQVMGNDVAVNFGGAQGHLELNVFKPVIAFDFLMSVRLLGDAMKSFEEHCARGIE
                     PNRERIDALVRGSLMLVTALAPHIGYDKGAAIAKKAHKEGTTLREAALALGHVTAEDY
                     DKWVRPEQMVGPMGR"
     gene            1780..2220
                     /locus_tag="VF309_01855"
     CDS             1780..2220
                     /locus_tag="VF309_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7465356.1"
                     /translation="MKSAFAAVILAATAVAVQAQQVKLEDQVKYRRAAYTLMSLNFGS
                     LAAMAQDKKPFNRDEAIRNADFVAMLSTVPKLYFGEGTDKDTKAKPEIWTNRADFDAK
                     MDKMAGEAQKLPAAVRAGDMAAFKKQVGDVGSACKACHDEYRLK"
     gene            2217..>2744
                     /locus_tag="VF309_01860"
     CDS             2217..>2744
                     /locus_tag="VF309_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7465357.1"
                     /translation="MIETGERIRVWDLPTRIFHGALAVLVVFSFVTGKIGGGWMEWHV
                     KSGCTILTLLVFRLAWGIVGSRTSRFTGFVTGPGAAIRYARETFAKRHPFTPGHNPLG
                     GWMVVFMIAILLLQAATGLFADDEIATQGPLAAKVSDALVSRLTTIHKYNVWLIAAAV
                     GLHLVAVSTYQWGLKV"
BASE COUNT          454 a          924 c          897 g          469 t
ORIGIN      
        1 gcggggtaat ttcgatcggg taacccacga ggagtaacga tccatgacca acaagaccct
       61 tttctcgatc gcggcgctcg ccggcttcgt gagctactgc ttctatcggg gcggccgcac
      121 cgcccacgcc gtgagacgcg aggcgaagca tgacctccat cgctgggagg gcgaaggcgg
      181 caatgtgccg caagtcgcga cggtatcgcc cgtcccggtt cccccgggcc cgcgtcccgg
      241 aaatggcgcg gcagcgagcc actgagcgct agccgggaac gccttccggg gggaatcccg
      301 ggcgctagcg acccatgggc ccgaccatct gttccggccg cacccacttg tcgtaatcct
      361 ccgcggtgac gtggcccagc gcgagcgcgg cttcgcgcag cgtggtgccc tccttgtgcg
      421 ccttcttcgc gatcgcggcc cccttgtcgt agccgatgtg gggcgccagc gcggtgacga
      481 gcatgaggga gccgcgcacg agggcgtcga tcctctcccg gttgggctcg atgccgcgcg
      541 cgcaatgctc ctcgaagctc ttcatggcgt cgccgagcag acgcacgctc atgaggaaat
      601 cgaatgcgat gacgggcttg aacacgttca gctcgagatg tccctgagcg ccgccgaaat
      661 tcacggccac gtcgttgccc atcacctggc acgcgcacat cgtgagtgcc tcgcattgcg
      721 tcggattcac cttgcccggc atgatcgagc ttccgggctc gttctcggga atcgtgatct
      781 cgccgatgcc gctgcgcggc ccgctcgcaa ggagccggac gtcgttcgcg atcttcatga
      841 gcgacgccgc gagccccttc aggctgccat gcgcatgcac catcgcatcg tgggtcgcga
      901 gcgcctcgaa cttgttgggc gcggtgacga actcgcaacc cgtgaggcgc gagatctcgg
      961 ccgccgccag ctctccgaag cgaggcggcg cgttgagccc cgtgcccacc gccgttccgc
     1021 cgagcgcgag ctccgacagg tggggaagcg ccgattcgac gtggcggcgc gagtgatcga
     1081 gctgcgcggc gtagcccgag aactcctggc ccagcgtgag cggggtcgca tcctgcaagt
     1141 gcgtgcggcc gatcttcacg atgcgcgcga actgctccga cttcgccgcg agcgtcgcac
     1201 gcagcgtctc cagcgccggc agcaggcgcc gccggatcga aacgaccgcc gccacgtgca
     1261 tcgcggtggg gaatacgtcg ttggagctct ggccgcggtt cacgtcgtcg ttcggatgca
     1321 ccaggcgccc ttccccgcgc gccccgccga gcatctcgct cgcgcgattg gcgagcacct
     1381 cgttcacgtt catgttggtc tgggtgcccg atccggtctg ccataccacc agcggaaatt
     1441 catcgggcca cttgccggcg agaacttcgt ccgcggcctg tgagatggcc ccggcctttt
     1501 ccgctgttaa cccgccgagg gagtggttca ccgtggcgca ggcgcgcttc acccacgcga
     1561 gcgcctggat gatctccgcg ggcatgcgct cgccggagat cctgaagtgc tcgagggagc
     1621 gctgcgtctg ggcgccccac aaacgctcgt cgggcacctc gatggggccg aaggtatcct
     1681 tctcgattct catgccggac atggtggctc tccgaagctg cagcccgatt ttcccgtaaa
     1741 gttgccgttc accaaaacaa cgaaaggaag cgcgcaatga tgaaatcagc gttcgccgcc
     1801 gtcattctgg cggccaccgc ggtcgccgtc caagcccagc aggtcaagct cgaggaccag
     1861 gtcaagtacc gcagggcagc ttataccctc atgtccctca acttcggcag cctcgccgcc
     1921 atggcccagg acaagaagcc gttcaacagg gacgaggcga tccggaacgc cgatttcgtc
     1981 gcgatgcttt cgacggtgcc gaagctgtac ttcggcgaag ggaccgacaa ggatacgaaa
     2041 gcgaagcccg agatctggac caaccgcgcg gatttcgacg cgaagatgga caagatggcg
     2101 ggcgaggccc agaagcttcc cgcggcggtg cgcgcggggg acatggccgc cttcaagaag
     2161 caggtaggcg acgtgggctc cgcgtgcaag gcgtgccacg acgagtatcg cctcaagtga
     2221 tcgagacggg cgagcgcatc cgggtgtggg atctccccac ccggatcttt catggggcgc
     2281 tcgcggtgct cgtggtgttt tcgttcgtca cgggcaagat cggcggcggg tggatggaat
     2341 ggcacgtgaa gagcggatgc acgatcctca cgctcctcgt cttccgcctc gcctggggaa
     2401 tcgtcggcag ccgcacatcg cgattcaccg gattcgtgac ggggccgggt gcggcgattc
     2461 gctatgcgcg agagacgttc gcgaagcgcc accccttcac gccgggccac aatcccctcg
     2521 gcggatggat ggtcgtcttc atgatcgcga tcctcctcct gcaggcggcg acgggcctgt
     2581 tcgccgacga cgagatcgcg acccagggtc ccctcgccgc caaggtgtcc gatgcgctcg
     2641 tgagccggct cacaacgatc cacaaataca acgtgtggct catcgccgcg gccgtcggcc
     2701 tgcacctcgt cgcggtctcg acctatcagt ggggcctcaa ggtc
//
LOCUS       DASVII010000142         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_294638,
            whole genome shotgun sequence.
ACCESSION   DASVII010000142 DASVII010000000
VERSION     DASVII010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294638"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VF309_01865"
     CDS             <1..591
                     /locus_tag="VF309_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX7465358.1"
                     /translation="MRRGDAGVIALGDLAMQGMHNAANAMAAHALLCAIEAPAAPLAR
                     AMREFKGLPHRVQLVAEANGIGFYDDSKGTNVGATVAALAGFRTPLVLIAGGDGKGQD
                     FAPLAPAVKSRARAAVLIGRDGARIAAALAGCGVPLARAGSLEEAVENAFALASPGDA
                     VLLSPACASFDMFRDYRHRGEMFAAAARAIAMRKGR"
     gene            591..1742
                     /gene="ftsW"
                     /locus_tag="VF309_01870"
                     /pseudo
     CDS             591..1742
                     /gene="ftsW"
                     /locus_tag="VF309_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411696.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
BASE COUNT          222 a          604 c          611 g          338 t
ORIGIN      
        1 atgcggcgcg gcgacgctgg cgtgatcgcg ctcggcgacc tggcgatgca gggaatgcac
       61 aacgccgcga acgcgatggc ggcgcacgcg ctgctctgcg cgatcgaggc gcccgcggcg
      121 ccgctcgcgc gggcgatgcg cgaattcaag ggattgcccc atcgcgtgca gctcgtcgcc
      181 gaggcgaacg gcatcggctt ctacgacgac tcgaagggga ccaacgtcgg cgccacggtc
      241 gccgccctcg caggcttccg cacgccgctg gtgctgatcg cgggcggcga cggcaaggga
      301 caggatttcg ccccgctcgc cccggccgtg aagtcgcgcg cccgcgctgc ggtgctgatc
      361 ggacgcgatg gcgcgcgcat cgccgcggcg ctcgccggat gcggcgtgcc gctcgctcgc
      421 gcaggctccc tggaggaagc ggtcgagaac gcattcgcgc tcgcgtctcc cggcgacgcg
      481 gtgcttctct cgcccgcgtg cgcgagcttc gacatgttcc gcgactatcg ccaccgcggc
      541 gagatgttcg ccgccgcggc gcgcgccatc gcgatgcgca aggggcgctg atgctctaca
      601 accccgctcg ccgccaatcg agcctcgacc cggcattgct gtggtcggcg ctcatgctcg
      661 gcgcgatcgg gctcgtcatg gtgtattcgg cctcgatcgc gatggccgag gcggagcgct
      721 tcacgggctt ccgcaccagc tactttctcg tgcgccactc gatctatctt ctggtcgggt
      781 tggcgtttgc cgcggcgctg tttcgcattc ccctctggct ctggcagagg gcgtcgccgt
      841 ggctttttct cgccggcacc gcgatgttgg tcgcggtgct ccttcccgga ttcggccgcg
      901 aggtgaacgg cgcgcggcgc tggatctcgc ttggcatcgg gaccttccag ccctcggagc
      961 tcatgaagct cttcgtggtc ctgtacgcgg cggactacac cgtgcgcaag gccgcgttca
     1021 tggacgacgt caagaaggga ttcctgccga tgttcgcggt gatgacgacc atcgcggcgc
     1081 tgctgctgcg cgagcccgac ttcggcgcgc tcgtcgtggt gaccgcgatc gcgatggcgg
     1141 tgctcttcct cggggggctc aactggaggc tcttcgccgg cctcgcggcg ctgctgggcg
     1201 tcgccttcgc ggtgctcatc gtctcgtcgc cctatcgcct gcaacgcatc ctcggcttca
     1261 tggatccgtg gaacgattcg tacggcaagg gctaccagct ctagcactcg ctgatcgcct
     1321 tcggccgcgg cgaatggttc ggcgtgggct tgggcgcgag cgtcgagaag ctcttctacc
     1381 ttcccgaggc gcacaccgat ttcctgctgg cggtaatcgc cgaggagctc ggcttcgtcg
     1441 gggtgctcgc ggtgatcctg ctgttcctgt tcctcgtgca tcgcgcgttc gcggtgggac
     1501 ggcaagccgc gagcctcgag cgctatttcg cggcgctcgt cgcgcaaggt atcggcgtgt
     1561 ggatcgcggg ccaggcgttc atcaacatgg gcgtgaacat ggggcttctc ccgacgaagg
     1621 gactgacgct cccgctcctg tcgttcggcg gcaccggcat cgtcgtgaac tgcatggcgc
     1681 tcgcgatcct gatgcgcatc gactgggaaa accgctacct gatgcgcggg ggttcgctgt
     1741 gagccgcacc atcctcatca tggccgcagg ttccg
//
LOCUS       DASVII010000143         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_366655,
            whole genome shotgun sequence.
ACCESSION   DASVII010000143 DASVII010000000
VERSION     DASVII010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366655"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VF309_01875"
     CDS             complement(<1..493)
                     /locus_tag="VF309_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX7465359.1"
                     /translation="MTRLVYVNGRYVPYAMAAVHAEDRGFQFADAVYEVCQVRGGHLV
                     DEARHMARLERSLAALEIARPMSAAALANVMRQTIKKNRVRDGLVYLQVTRGAGPREF
                     SYPAPDVPRTVVCLARSMSAASQEANALTGIAVKTMPDIRWRRSDIKTVMLLPACLAK
                     NAAQ"
     gene            complement(535..>1620)
                     /locus_tag="VF309_01880"
     CDS             complement(535..>1620)
                     /locus_tag="VF309_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7465360.1"
                     /translation="KPFKADRLILVTLRALEAFQLKREVKELRERSSFSAEMIGKSSA
                     INQLRNALDRVAPTNSRILIRGPSGAGKELAARVIHVKSARADSPFVVLNAAAMAPDR
                     VEEEMFGTEDRSGAPRKVGALEEAHGGTLYIDEVADMPMETQGKVLRVLVEQKFLRLG
                     GSQKVAVDVRIISSTSRDLEREISEGRFREDLYHRLNVVPLRVPSLAERREDIPDLIQ
                     YFVGQLSSASGLAPRRIGEDAIAVLQAHDWPGNVRELRNNIERLMILAGGDPNTVITA
                     EMLPEEIGSNVPLPVNGGAEHLMSLPLREAREIFEREYLLAQINRFGGNISRTAEFVG
                     MERSALHRKLRALGVSSEHRSPEARQA"
BASE COUNT          270 a          533 c          542 g          275 t
ORIGIN      
        1 gctgggctgc gttcttggcg agacaggctg gcagcagcat caccgtcttg atgtcgctgc
       61 ggcgccagcg gatgtcaggc atcgtcttga ccgcgatgcc ggtaagggcg ttcgcctcct
      121 gggaagcggc cgacatcgag cgcgcgaggc acaccaccgt gcgcggaaca tcgggcgctg
      181 gatacgagaa ctcgcgtggc ccagcgccgc gtgtaacttg caggtacacg agaccgtccc
      241 tgacgcggtt tttcttgatc gtctggcgca tcacgttggc gagcgcggct gcgctcatcg
      301 ggcgggcgat ctcgagagca gcgagcgaac gctcgagccg cgccatgtgc cgcgcctcat
      361 cgacaaggtg cccgccgcgc acctggcaca cctcatacac ggcatcggcg aactggaagc
      421 cgcggtcctc cgcgtgcacg gcggccatcg cgtaaggcac gtagcggccg ttgacataga
      481 cgagacgggt cacgacgccc tcagatgaca gctagaccgc gcgctttgcg ccattcacgc
      541 ttggcgcgcc tcggggctac ggtgctccga tgagacgccg agcgcccgca gcttgcggtg
      601 cagcgcggag cgctccatgc cgacaaactc cgcggtgcgc gatatgttgc ccccgaagcg
      661 gttgatctgt gccagcaaat attcgcgctc gaagatttcg cgcgcctcgc gcagaggcag
      721 cgacatcaga tgctcggcgc cgccgttgac gggcagcggc acgttcgagc cgatttcctc
      781 gggcagcatc tcagccgtaa tgacggtgtt gggatcgccg ccggcgagga tcatgagccg
      841 ctcgatgttg ttcctcaatt cgcgcacgtt gcccggccag tcgtgcgcct gcagcaccgc
      901 aatggcatcc tcgccgatgc ggcgcggagc aagccccgag gcgctgctca gctgacccac
      961 gaaatactgg atcaaatccg ggatgtcctc gcgccgctcg gcgaggcttg gaacccgcag
     1021 cggcacgaca ttcaagcgat ggtagagatc ctcgcggaag cggccctcgc tgatctcgcg
     1081 ttcgagatcg cggctggtcg aggagatgat gcgcacgtcg accgccactt tctgcgagcc
     1141 gccgaggcgc aggaatttct gctcgacgag gacgcggagc accttgccct gtgtctccat
     1201 cggcatgtcg gcgacctcgt cgatgtagag cgtcccgccg tgcgcttcct cgagcgcgcc
     1261 aaccttgcgc ggcgcaccgg agcgatcctc ggtgccgaac atctcctcct cgacgcggtc
     1321 gggcgccatc gcagcagcgt tgagcacgac gaagggcgaa tctgcgcgcg cggacttcac
     1381 gtgaatgacg cgcgcggcga gctccttgcc ggcacccgac ggcccgcgga tgagaatgcg
     1441 gctgttggtt ggggcgacgc ggtcgagcgc gttgcgcagc tggttgatcg cggaggattt
     1501 gccgatcatc tcggccgaga agctcgaacg ctcgcgcagc tccttgacct cgcgcttcaa
     1561 ctggaacgct tccagcgcgc gcagagtgac gaggatcagg cggtcggcct tgaacggctt
//
LOCUS       DASVII010000144         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_582311,
            whole genome shotgun sequence.
ACCESSION   DASVII010000144 DASVII010000000
VERSION     DASVII010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582311"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..475
                     /locus_tag="VF309_01885"
     CDS             155..475
                     /locus_tag="VF309_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7465361.1"
                     /translation="MTPPKSTEPCPVARTLDIVGDRWTILILRELLTNPSRRFQDLET
                     SLRGISPNTLSARLKNLEEHAIVTRRLYEEHPPRAEYVLTPKGRELGPALQALLVWGR
                     KHTG"
     gene            479..2143
                     /locus_tag="VF309_01890"
     CDS             479..2143
                     /locus_tag="VF309_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7465362.1"
                     /translation="MGTPARAGSVSAALAVRVAFGCALSALSAGMLTLAFAPHRAWYL
                     VWIGFVPMLVAQHRVLPGRLSALAPGIAVGGFVAGCFGGVFPERAAWYMKQLPPLVGV
                     IIALSSLGGPTGRARAGYGYWPLQGAIGWVAVELVRSLIPALGTWGFLGYALYRQTWL
                     LQPVAIFGTFGLDLLVVLVSYALALLLFAWLDRRRGRNRNDPLDGERAFVPMRLAVRW
                     CAGVAAACVLWGALSVAMLPRGARAPTVRVAALQPGYRWKANVHSREERDRAILEALA
                     DQTRRAAAQGARFIVWPESALHADPQIVYREPLAALAKEVSAYLFVGYLLDTPAGRRN
                     EVVTIAPQDGFLGAYGKDHPVGFLGEASITRGTYPTYATPFGVVGAVICADIDFTDTP
                     RKLARNGAKILFVPSADWPKISTKHYVLAVFRALETGSAVVKSEYGRDSAIVDGYGAM
                     LASVVTPEGSEAVLVADVAARPGLPLAARWGDWVGWLCVAALVVRGLARLRGRGTPKL
                     SAAGGAAAARRGGGGAGGERAAECGEHGRRASPVPRPPRGAPTVRA"
BASE COUNT          280 a          757 c          769 g          360 t
ORIGIN      
        1 gagggcgctc cgccctccaa ccgcgcgggt agcacgcaaa tgaaccagcg cgcgtgggcc
       61 tcttcgcgga aggtgttgat ggacaagctc ggcgcgagct tcgagcagta aacgaccatt
      121 tactatcgaa ggtgtagttg ctataactag aatagtgaca cctccgaaga gcaccgagcc
      181 gtgccctgtc gcgcgcacgc tcgacatcgt cggcgaccgc tggacgatcc tcatcctgcg
      241 tgagctgctg acgaacccgt cgcgccgctt ccaagatctc gagacgtccc tccgcggcat
      301 cagcccgaac acgctctcgg cgcggctgaa gaacctcgag gagcacgcga tcgtcacgcg
      361 tcgtctctac gaggagcacc ctccgcgcgc cgagtacgtc ctcacgccga aggggcgcga
      421 gctcggtccg gccctccagg cgctcctcgt ctggggacga aagcacacgg gttgagccgt
      481 gggcacgcct gctcgcgccg gctccgtctc cgccgccctg gcggtgcgcg tcgccttcgg
      541 gtgcgcgctc tcggcgctga gcgcggggat gctcacgctc gcgttcgcgc cgcaccgcgc
      601 gtggtacctc gtgtggatcg gcttcgtgcc gatgctcgtc gcgcagcatc gcgtcctgcc
      661 cgggcgtctg tcggcgctcg cacccggtat cgccgtcgga ggcttcgtcg ccgggtgctt
      721 cggcggcgtc ttccccgaga gggcggcctg gtacatgaag cagcttccgc cgctggtggg
      781 cgtgatcatt gcgctctcga gcctcggcgg acctacgggt cgcgcgcgcg cgggatatgg
      841 ctactggccg ctgcaagggg cgatcggttg ggtcgccgtc gagctcgtgc gctcgctcat
      901 ccccgcgctc gggacgtggg gcttcctcgg ctacgcgctt tacaggcaga cctggctcct
      961 gcaaccggtc gcgatcttcg gcacgttcgg gctcgacctt ctcgtcgtgc tcgtgagcta
     1021 cgcgctcgcc ctgctgctct tcgcgtggct cgatcgacgg cgaggtcgca atcgcaatga
     1081 ccctctggac ggcgaacgcg cgttcgtccc gatgcggctc gccgtgcgtt ggtgcgcggg
     1141 cgtcgccgcc gcgtgcgtgc tgtggggagc gctgagcgtc gccatgcttc cgcgcggggc
     1201 gcgcgcgccc acggtgcggg tcgcggcgct gcaacccggc tatcgatgga aggcgaatgt
     1261 ccactcgcga gaggagcgcg atcgcgcgat ccttgaggcg ctcgccgatc agacacggcg
     1321 cgccgccgcg caaggggcgc gattcatcgt ctggcccgag tcggcgctgc acgccgatcc
     1381 tcagatcgtc taccgcgagc cgctcgctgc gctcgcgaag gaggtctctg cctatctctt
     1441 cgtcggctac ttgctcgaca cgcccgcggg tcgtcgcaac gaggtcgtca ccatcgcccc
     1501 ccaagacggg ttcctcggcg cctacggcaa ggatcacccg gtcggcttcc tcggcgaggc
     1561 cagcattacg cgcgggacct accctacgta cgcgacgccg ttcggcgtgg tcggcgccgt
     1621 catctgcgcc gacatcgact tcaccgacac gccgcgcaag ctcgcgcgca acggcgcgaa
     1681 gatcctcttc gtaccgtcgg cggattggcc caagatctcg accaagcact acgtcctcgc
     1741 ggtcttccgc gcgctcgaga cgggttccgc ggtcgtgaag tccgagtatg gacgcgactc
     1801 cgccatcgtc gacggctatg gagcgatgct cgcgagcgtc gtcacgcccg aggggagcga
     1861 ggccgtgctc gtcgccgacg tcgccgctcg accgggcctg ccgctcgccg cgcgctgggg
     1921 ggactgggtg ggttggctgt gcgtggctgc gctcgtcgtg cgcgggctcg cgcggctgcg
     1981 gggtcgcggg acgccgaagc tgagcgcggc gggaggcgca gcggcagcga ggcggggggg
     2041 cgggggggcg gggggtgagc gcgcggcgga gtgcggagag cacgggcgtc gagcatcgcc
     2101 ggttcctcgc ccgccccgag gcgcaccgac cgttcgcgcg tagcgccata ttcgagcgcg
     2161 ccggcg
//
LOCUS       DASVII010000145         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_474407,
            whole genome shotgun sequence.
ACCESSION   DASVII010000145 DASVII010000000
VERSION     DASVII010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474407"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..857
                     /locus_tag="VF309_01895"
     CDS             177..857
                     /locus_tag="VF309_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465363.1"
                     /translation="MKSICTRRCAEHSHPEIRLEYDSALALTADALWLSDALEMQVAA
                     GERYEEGETFQVGWALTRFRKDPDGFLSLEEPDMTSMPIQWQRGVTRTLAHLRRQRDV
                     VASVLGTDVPAFPSLLQSCLVCTRVTHRDASVMMAREVPEGNDSGWYIGCMDARHDHN
                     DPAQLKRVSLYEAIVAHAPQAVGYLALPPGATAIVGKARPVIRVDNKELVFIPGSYLA
                     TRYDTKGG"
     gene            complement(861..>1671)
                     /locus_tag="VF309_01900"
     CDS             complement(861..>1671)
                     /locus_tag="VF309_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465364.1"
                     /translation="QQSNIIFVAMYTYDASGNPVWYVVSNCPILAGGCTGSLFRVRGG
                     TQPTRPWSGSGIAVSTVGSMTLAFSDVNNATMSFSIDGVNGFKTITRQIFVAPTPPVN
                     TNLAKSTQLVGGAWTYVYTIVSTFTDTMRFTSVDTTPSATGTYYAHGTDQFGGLVIGG
                     YDPTVSTWLVVDPGVIIDQAYVFNFSDNNDVTGCYYQINPPGSANLSRCYSLFGFRSP
                     AQADAPAAKTRADAYARELQVSSEVMMDRKAGVAADPRVVESYLEARKSIR"
BASE COUNT          275 a          523 c          571 g          302 t
ORIGIN      
        1 cttcggtgtc caccaggagg atcggcattg cgaggcagct cgcgagctgg cgggcgagga
       61 tgatctggat gggtttcgag gaggccatgg cgctttgcta tggtcggcgc tgcgtggtcg
      121 cttgtctgtc cgatgccgca cataggggcc aggctcggcc ccgttatcat tgggggatga
      181 agtccatctg cacgcggcga tgcgccgagc attcccatcc cgagatccgc ctcgagtacg
      241 acagcgcgct cgcgctcacg gccgatgccc tgtggctctc ggacgcgctg gaaatgcagg
      301 tcgcggcggg cgagcgctac gaggagggcg agaccttcca ggtcggctgg gcgctgacgc
      361 gcttccggaa ggatccggac ggcttcctga gcctcgagga acccgacatg acctcgatgc
      421 ccatccagtg gcagcgcggg gtgacgcgga ccctcgcgca cctgcgccgg cagcgcgacg
      481 tggtcgcgag cgtgctgggc accgacgtgc ccgcgtttcc ctcgctcctg cagtcgtgcc
      541 tcgtatgcac ccgggtcacg caccgcgacg cgagcgtgat gatggcccgc gaggttcccg
      601 agggcaacga ctcgggctgg tacatcggct gcatggatgc gcggcacgat cacaacgacc
      661 cggcgcagtt gaagcgggtg tccctctacg aggcgatcgt cgcccatgcg ccgcaggccg
      721 tgggatacct cgcgctgccg ccgggcgcga cggcgatcgt cgggaaggcg cgccccgtca
      781 tccgcgtgga caacaaggag ctcgtgttca ttccgggctc ctacctcgcg actcggtacg
      841 acaccaaggg cggctgagtc ctagcgaatg ctcttcctcg cctcgaggta gctctcgacc
      901 acgcgagggt cggcggcaac gccggctttt cgatccatca tcacctccga cgaaacttgc
      961 agctcgcgcg cgtaagcgtc ggcccgggtc ttggcagccg gggcatcggc ctgcgcaggc
     1021 gacctgaatc cgaagaggct atagcagcgg ctcaggttcg cgcttcccgg cgggttgatc
     1081 tggtagtagc acccggtgac gtcattgttg tcggagaagt tgaatacgta ggcctggtcg
     1141 atgatgaccc ccggatcgac caccagccac gtcgagacgg ttggatcgta tccaccgatc
     1201 accagtccgc cgaactgatc ggtcccgtga gcgtagtacg ttcccgtggc gctcggcgtc
     1261 gtgtccacgc tcgtgaaccg catggtgtcg gtaaacgtgc tgacgatcgt gtagacgtag
     1321 gtccacgcgc ctccgacaag ctgcgtgctc tttgcgaggt tggtgtttac cggcggcgtc
     1381 ggcgcgacga agatttgtcg cgtgatggtc ttgaagccgt tgacgccgtc gatcgagaaa
     1441 gacatggtgg cgttgttgac atccgagaag gcgagggtca tcgatcccac cgtggagacc
     1501 gcgatgccgg agccgctcca tggccgcgtg ggctgcgtgc cgccgcgcac ccggaagagc
     1561 gagcccgtgc atccgccggc gaggatcgga caattggata cgacgtacca caccggattt
     1621 cccgatgcgt cgtaggtgta catggccacg aagatgatgt tcgactgctg a
//
LOCUS       DASVII010000146         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_510221,
            whole genome shotgun sequence.
ACCESSION   DASVII010000146 DASVII010000000
VERSION     DASVII010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510221"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF309_01905"
     CDS             <1..240
                     /locus_tag="VF309_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7465365.1"
                     /translation="PAELFEREQTEAMNSRGLSAALQSLDERSRRIVRARWLAESDPK
                     TLHELAVEFNVSAERIRQIEAKAMQKMKKQMATLH"
     gene            complement(320..910)
                     /locus_tag="VF309_01910"
     CDS             complement(320..910)
                     /locus_tag="VF309_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7465366.1"
                     /translation="MRSSALALACAAFLAAACDLAGEARTPFKGIDVTGSPMGRQLRL
                     TDHNGQPRSLEDFRGKVVVVTFGYTQCPDVCPTTLADLSSAVKKLGADASRVQVLFVT
                     IDPKRDKPELLKQYVPAFNPAFLGLYGDAAATKAATSEFKVYAQERAGKTPETYTMDH
                     SAQIFVLGKDGRLRLVEAFGATPDAMASDLRVLLDS"
     gene            complement(916..>1723)
                     /gene="cyoE"
                     /locus_tag="VF309_01915"
     CDS             complement(916..>1723)
                     /gene="cyoE"
                     /locus_tag="VF309_01915"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7465367.1"
                     /translation="AVIGMFLATPGLPPPALVAWATLGIALVAGAAAAFNCLVEQKID
                     AAMARTRGRPTVMGTISSSQVVIFAGIMGGLGLAILWHAVNPLTMWLTLGTFVGYAVI
                     YTVILKPATPQNIVIGGASGAMPPVLGWAAITGDVTYQPLLLFLIIFAWTPPHFWALA
                     LYRRNEYARAGVPMLPVTHGEAFTRLHLLLYTFLLTAATLLPFASGMAGFFYLGVALV
                     LDAAFLGYSVAIWRHYSDALAKRTFAYSIFYLAMLFAALMVDHYLAVFAG"
BASE COUNT          321 a          557 c          604 g          241 t
ORIGIN      
        1 cccgccgagc tgttcgagcg cgagcagacc gaggcgatga acagccgcgg gctttccgcg
       61 gcgctgcaga gcctcgatga gcgcagccgc cgaatcgtgc gggcacgctg gctcgcggag
      121 tcggatccga agacgctgca cgagctcgcc gtcgaattca acgtatcggc cgagcgtatc
      181 cgccagatcg aagcgaaggc gatgcagaag atgaagaagc agatggcgac gctccactga
      241 ggtcgccggc gagccattcg caaacggggc ttcggccccg ttcttgtttg gggatggctc
      301 cgcctcgaag cgttcgctcc taggagtcga gcaggaccct caagtccgag gccatcgcat
      361 cgggcgtcgc gccgaaggct tccacgagcc gcaggcgccc atcttttccg agcacgaaga
      421 tctgcgcgct gtgatccatc gtgtacgtct cgggcgtctt ccccgcccgc tcctgggcgt
      481 agaccttgaa ctcgctcgtc gccgccttgg tcgccgcggc gtcgccatag agccccagga
      541 aggccggatt gaacgccggc acgtattgct tgaggagctc cggcttgtcg cgcttcggat
      601 cgatcgtcac gaacaggacc tgcacgcgcg aggcgtcggc gcccagcttc ttcaccgccg
      661 aggacagatc cgcgagcgtc gtgggacaaa cgtccgggca ttgggtatag ccgaacgtga
      721 cgacgacgac cttgccgcga aaatcctcga gcgatcgggg ctggccgttg tgatcggtca
      781 ggcgcaactg ccttcccatc ggggagccgg tgacgtcgat ccccttgaag ggcgtgcgcg
      841 cttccccggc caggtcgcac gccgcggcga ggaacgcggc gcaagcgagc gcgagcgcgg
      901 acgagcgcaa gcgcctcacc cggcgaacac cgccaggtag tggtcgacca tgagcgcggc
      961 gaagagcatg gcgaggtaga agatcgagta ggcgaacgtg cgcttggcaa gggcatcgct
     1021 gtagtggcgc cagatcgcga ccgagtagcc gaggaacgcc gcgtcgagca cgagcgcgac
     1081 gccgaggtag aagaagcccg ccatgcccga cgcgaacggc agcagcgtgg cggccgtgag
     1141 gaggaacgtg tacagcagca ggtgcagcct cgtgaacgcc tcgccgtggg tgacgggaag
     1201 catcggcacg ccggcacgcg cgtactcgtt gcgccggtag agcgcgagag cccagaagtg
     1261 cggcggcgtc cacgcgaaga tgatgaggaa gaggagcagc ggctggtagg tcacgtcgcc
     1321 cgtgatcgcg gcccagccga gcaccggagg catggcgccc gaagcgccgc cgatcacgat
     1381 gttctgcggc gtcgcgggct tgaggatcac cgtgtagatc acggcgtatc cgacaaaggt
     1441 gccgagcgtg agccacatcg tgagcggatt caccgcgtgc cacaggatcg cgagcccgag
     1501 gccgcccatg atgcccgcga agatcacgac ctgcgaagac gagatggtgc ccatgacggt
     1561 ggggcggccc cgcgtgcgcg ccatagcggc gtcgatcttc tgctcgacga ggcaattgaa
     1621 ggcggccgcc gctcccgcca ccagcgcgat tcccagtgtc gcccacgcca ccagcgcggg
     1681 cggaggcaac ccgggagttg cgaggaacat cccgatcacc gcc
//
LOCUS       DASVII010000147         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_330816,
            whole genome shotgun sequence.
ACCESSION   DASVII010000147 DASVII010000000
VERSION     DASVII010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330816"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..819)
                     /locus_tag="VF309_01920"
     CDS             complement(43..819)
                     /locus_tag="VF309_01920"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7465368.1"
                     /translation="MSVANPKASLSARDQVSKDEWEARVNLAAAYRLVALYGWDDLIF
                     THISARVPGPDHFLINPYGWMFEEITASSLVKVDLHGSKVMPSAYDVNPAGFTIHSAV
                     HAAREDAICVMHTHTPNGVAVSAQKNGLLPISQQSLGVLSSLGYHDYEGLALNEDEKP
                     RLVRDLGDKNFLMLRNHGLLTAAASIAEAFAMMYFFEVSCMVQLRAQSGGSELIPIPK
                     PVLDGMRIAVNKVTRGAGPGALAWPALLRKLDRLDPSFRD"
     gene            1026..>1845
                     /locus_tag="VF309_01925"
     CDS             1026..>1845
                     /locus_tag="VF309_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7465369.1"
                     /translation="MRYLFEDCVFDTDRRELRRGVDAVPVAPQVFDMLAYLIRNRERV
                     VSKDDLIAAIWDGRIVSDAAMTTRLNVVRGAIGDSGEEQRLIKTLPRKGIRFVGTVRE
                     EGESASPVAAGASAEAPGTGLTLPDKPSLAVLAFANLSSDPEQEYLADGIVEDIITEL
                     SRFSELFVIARTSSFQYKGKAADVRQVGRELGVRYVLEGSVRCRGDRLRISAQLIDAM
                     TGGHRWAEHFDRTLEDVFAVQDEVVRAIVAILAAHVRKAETERTRVRPPNSWQAY"
BASE COUNT          344 a          556 c          598 g          347 t
ORIGIN      
        1 caatagcctt tcctctggcc acctgcacgc aatacgcagt agctaatccc tgaatgacgg
       61 atccagccga tccagcttgc gaaggagtgc tggccaggcg agtgcgccag ggccggcccc
      121 gcgagtgact ttgttcaccg cgatccgcat gccatcgaga acgggtttgg ggatcgggat
      181 cagttcgctg ccaccggatt gcgcgcggag ctggaccatg cagctcactt caaaaaagta
      241 catcattgcg aaggcctcgg cgatggaggc ggccgcggtg agcaggccgt gattgcgcag
      301 catgaggaag ttcttgtcgc cgagatcgcg cacgaggcgc ggtttttcat cctcgttgag
      361 cgcgaggccc tcgtagtcgt gatagccgag tgaagaaagc acgcccagcg actgttgcga
      421 gatcggcagc aggccgttct tctgcgcgga caccgcgaca ccattgggtg tatgagtgtg
      481 catcacgcat atcgcgtcct cgcgcgcagc gtgaaccgcg ctgtgaatgg tgaagccggc
      541 aggattgacg tcgtatgccg agggcatcac cttgctccca tggaggtcca ctttcaccag
      601 cgaagacgcg gtgatctcct cgaacatcca gccgtacgga ttgatgagaa agtggtctgg
      661 tcccggcacg cgagctgaga tgtgggtaaa gatgagatca tcccagccgt agagcgcgac
      721 gaggcggtag gcagcagcga ggttgacgcg cgcctcccat tcatccttgc tgacttgatc
      781 gcgggcgctg agggaggcct tagggttagc gacgctcatg gcagtctcct tcttgctgaa
      841 gtgtgcaccg ggggtctcat cgcgatacgt tcggcggacg ttctcacgtg ggatcaggat
      901 gccctgaagc ggacgggcct gcctaaccgg cggcttacat tttcctcacc ggcgcctgct
      961 tttcgtgttc caagcccgtg ctaaggtggg ccggacgatc cgatacccaa aataggaacg
     1021 gcggcttgcg ttatctcttc gaggattgcg tgttcgacac cgaccggcgg gagctgcgtc
     1081 gcggggtgga cgcagttccc gttgcgccgc aggtgttcga catgctcgct tacctgatcc
     1141 gtaaccgcga gcgcgtggtc agcaaggacg accttatcgc cgccatctgg gacgggcgca
     1201 tcgtctccga cgcggcgatg accacccgtc tcaacgttgt tcgaggcgcg atcggcgaca
     1261 gcggcgagga acagcgcctg atcaagacac tgccgcgcaa gggtatccgg ttcgtgggta
     1321 ccgtgcggga agagggcgag tccgcaagcc cggtagccgc cggcgcatcg gcggaagccc
     1381 cggggaccgg cctgaccctc ccggacaagc cctccctcgc cgtgctcgcc tttgccaatc
     1441 tgagctccga tcccgagcag gagtatcttg ccgacggcat cgtcgaagac atcatcaccg
     1501 agctgtcgcg tttctccgag ctctttgtca tcgcccgcac ttccagcttc caatacaagg
     1561 gcaaagcagc cgatgtccgc caggtggggc gtgagctggg cgtgcgctac gtgctggagg
     1621 gcagcgtacg gtgcagaggc gatcgcttgc gcatttcggc tcagctgatc gacgccatga
     1681 caggaggaca ccgatgggca gagcacttcg accgcacgct cgaggacgtc ttcgccgtcc
     1741 aggatgaggt ggtgcgcgcg attgttgcaa ttctcgccgc acacgtccga aaggccgaga
     1801 ccgaacgcac gcgcgtcagg ccgcccaata gttggcaggc atatg
//
LOCUS       DASVII010000148         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_115078,
            whole genome shotgun sequence.
ACCESSION   DASVII010000148 DASVII010000000
VERSION     DASVII010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115078"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VF309_01930"
     CDS             complement(<1..247)
                     /locus_tag="VF309_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465370.1"
                     /translation="MDPTALKCGCPGCDGRACAVAAPVAWKTRESREHAWLDRLFLHP
                     RWGLVGSMLVFGGVLFVVFQGSAWRDAMTTARLADALS"
     gene            complement(292..>1611)
                     /locus_tag="VF309_01935"
     CDS             complement(292..>1611)
                     /locus_tag="VF309_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX7465371.1"
                     /translation="AAVSMPGMMETILDVGLNDATARGLLRLTGNPRLAWDSYRRLVQ
                     SFAEVVHGVPAARFAAALDERLRASRVDSARDLDFQALKSLTNDYLEVFEGAAGIPFP
                     QNPLDQLDAAVAAVWRSWSGAKAAAYRGMNALSDDVGTAVTVQSMVYGNAGGTSGAGV
                     AFTRDPASGEPRLYLDFLFNAQGEDVVSGRLGAPDAERMRHMLPALGEELARVASRLE
                     QEFRDMQEFEFTIQDGRLHILQARTGKRSSWAQLRIAIDQVDEGLIDEASAVKRLAHL
                     EAEKVARARVVRSADARVLCKAVIANQGVAVGEIVFDPAKAAEVAGAGRSVILVRGNT
                     ATEDIVGIAAAAGILTATGGRTSHAAVVARQLDKVCLVGCEALVMDAGGNGCTIAGER
                     FREGDVISLDGHSGEVIAGEAQMELERPVEGLARLRQWREGSAEPPV"
BASE COUNT          242 a          596 c          550 g          223 t
ORIGIN      
        1 cggagagcgc gtcggccagg cgtgcggtgg tcatggcatc gcgccacgcg ctcccctgga
       61 agacgacgaa cagcacgccg ccgaagacca gcatgctgcc gaccaggccc cagcgcggat
      121 gcaggaagag ccggtcgagc caggcatgct cgcgggactc gcgggtcttc cacgcaacgg
      181 gcgcggccac ggcgcaggcc cggccgtcgc agccggggca gccgcatttc aaggcggtcg
      241 gatccatcgc tccactttgg ccgcaaaggc gcccgcaccg cttgattcgg gtcaaacggg
      301 gggctcggcg ctcccctcac gccattgccg aagccttgcc agcccctcga ccgggcgctc
      361 gagctccatc tgcgcctcgc cggcgatcac ctcccccgag tgcccgtcga gcgagatcac
      421 gtcgccttcg cgaaacctct cgcccgcgat ggtgcagccg ttgcccccgg cgtccatgac
      481 cagggcctcg cagcccacca ggcagacctt gtcgagctgc cgcgcgacga ccgcggcgtg
      541 cgaagtgcgc ccgccggtcg cggtgaggat gccggcggcc gccgcgatac cgacgatgtc
      601 ctcggtcgcg gtgttgccac gcaccaggat cacggaacgg ccggcaccgg cgacttccgc
      661 ggccttggcg ggatcgaaga cgatctcgcc cacggccacg ccctggttcg cgatcaccgc
      721 cttgcacagg acgcgcgcat ccgccgaacg aacgacgcgc gcgcgcgcga ccttttcggc
      781 ctccaggtgc gcgaggcgct tcaccgcgct cgcctcatcg atgagccctt cgtccacctg
      841 gtcgatcgcg atcctcagct gggcccagga gctgcgcttg ccggtgcgcg cctggaggat
      901 gtgcaggcga ccgtcctgga tcgtgaactc gaattcctgc atgtcgcgga attcctgctc
      961 gagccgggag gcgacgcgcg cgagctcttc gccgagcgcc gggagcatgt ggcgcatgcg
     1021 ctcggcgtcg ggtgcgccga gccgtcccga cacgacgtcc tcgccttgcg cgttgaaaag
     1081 gaagtcgagg tagagccgcg gctcgccgct cgccggatcg cgcgtgaagg ccacgcccgc
     1141 gccggacgtt ccgcccgcat ttccgtagac catcgactgc accgtcaccg ccgtcccgac
     1201 gtcgtcggag agcgcgttca tcccgcggta agccgccgcc ttcgcgccgc tccacgatcg
     1261 ccacaccgcg gcgaccgcgg cgtcgagctg gtcgagcgga ttctgcggaa agggaatgcc
     1321 ggccgcgccc tcgaagacct cgaggtaatc gttggtgagg ctcttcaacg cctggaagtc
     1381 gagatcgcgt gcggagtcga cgcgcgaggc gcgcaggcgc tcgtcgaggg ccgccgcgaa
     1441 gcgcgcggcc ggcacgccgt gcaccacctc ggcgaacgac tgcacgaggc gccgatacga
     1501 gtcccacgcg agccgcggat tgccggtgag gcgcaggagc ccgcgcgcgg tcgcatcgtt
     1561 caggcccacg tcgaggatgg tctccatcat gccgggcatc gagaccgcgg c
//
LOCUS       DASVII010000149         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_78929, whole
            genome shotgun sequence.
ACCESSION   DASVII010000149 DASVII010000000
VERSION     DASVII010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78929"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..2675)
                     /gene="lon"
                     /locus_tag="VF309_01940"
     CDS             complement(252..2675)
                     /gene="lon"
                     /locus_tag="VF309_01940"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7465372.1"
                     /translation="MGDEKQTKDKAAGKGLYPVLPLRDIVVFPYMIVPLFVGRDKSIN
                     ALEEVMRSDKHILLAAQKNAGDDDPATDAIYPVGTLASVLQLLKLPDGTVKVLVEGTS
                     RARIARYTENPDYFEAEVEQVPEETGAKDEIEALARSAVTQFESYVKLNKKISPEVLG
                     NVAQIEDYSKLADTIASHLAIKIADKQEILETATISERLERVYTLMESEISVLQVERK
                     IRSRVKRQMEKTQREYYLNEQMKAIQKELGDSDERDDIAEFEEKIENTKLSKEARDKA
                     LAELKKLKQMSPMSAEATVVRNYLDWLLSIPWGVKGKVKKDLDEAQQTLDAEHYGLDK
                     VKERIVEYLAVQARTNKLKGPILCLVGPPGVGKTSLGKSMANATGREFVRMSLGGVRD
                     EAEIRGHRRTYIGSMPGKVIQSMRKAKRVNPLFLLDEIDKMGSDFRGDPAAALLEVLD
                     PEQNSTFNDHYLEVDYDLSNVMFVTTANTLNIPPALMDRMEIIRLAGYTEDEKVEIAK
                     RHLIPGQRLAHGLEADEWTIEDAALLTIVRRYTREAGVRSLEREIAKLARKAVKEILT
                     NTKKTVVVTAENLEGYLGVTKYRYGEAELEDQIGIVTGLAWTEVGGELLTIEGVMMPG
                     KGKMTVTGNLRDVMKESIQAANAYVRSRSIDFGIKPTLFAKRDIHVHVPEGATPKDGP
                     SAGVAMVTTIVSVLTANPVRRDVAMTGEVTLRGRVLPIGGLKEKLLAALRGGIKTVII
                     PEENVKDLTEIPVEIKNKIDIVPAARVDDVLKAGLVRVPERIIWDEAAEAQAVKNEDG
                     STGSRITAH"
BASE COUNT          426 a          882 c          860 g          556 t
ORIGIN      
        1 ggatggggtc ttggggggtc ttaaggcggc cggaaggccc taaatacggg ctctgcagcc
       61 tcaacagagg tggacaaaag cgcccaataa aacgcctcgt caagggggaa atcgcgcgaa
      121 attgcccgaa aaacctggga aaaacgcttg cttcgcgcac ggagcgggaa caaaaacccc
      181 gcaccttgcg acgcggggta tctgctggaa aaactggcag gcgccttagg ttgcgcctgc
      241 accaaattgt gtcagtgggc ggtgatccgc gagccggtcg atccgtcctc gttcttgacg
      301 gcctgggctt cggccgcctc gtcccaaatg atccgctccg ggaccctgac gagtccggcc
      361 ttgaggacgt cgtccacacg cgcggccgga acgatgtcga tcttgttctt gatctccacc
      421 gggatctcgg tcaggtcctt aacgttctct tcaggaataa tcacggtctt gatcccaccg
      481 cgcagggccg ccagcagctt ctccttcaag ccgccgatcg gcagcacgcg gccgcgcagg
      541 gtgacctcgc cggtcatggc gacgtcgcgg cgcaccggat tggcggtcag gaccgagacg
      601 atcgtggtca ccatggcgac gcccgcactt ggcccgtcct tcggcgtggc gccttccggc
      661 acgtggacgt ggatgtcgcg cttggcgaac agggtcggct tgatgccgaa gtcgatggag
      721 cgagagcgca cgtaggcgtt cgcggcctgg atcgactcct tcatcacgtc gcgcagattg
      781 cccgtgaccg tcatcttgcc cttgccgggc atcatgactc cttcgatggt cagcagctcg
      841 ccgccgacct cggtccaggc gagacccgtg acgatgccga tctggtcctc gagctcggcc
      901 tcgccgtaac ggtacttcgt cacgccgagg taaccctcga ggttctcggc ggtgacaacc
      961 accgtcttct tggtgttggt caggatctcc ttgaccgcct tgcgggcgag cttggcgatc
     1021 tcgcgctcga gcgagcgcac accggcctcc cgcgtgtagc ggcgcacgat ggtgagcagc
     1081 gcggcatcct cgatggtcca ctcgtccgcc tccaggccgt gggccaggcg ctgtccggga
     1141 atcaagtggc gcttcgcgat ctcgaccttc tcgtcctcgg tgtagccggc gagacggatg
     1201 atctccatgc ggtccatgag ggccggcgga atgttgagcg tgttcgccgt cgtcacgaac
     1261 atcacgttcg acaggtcgta gtcgacctcg aggtaatgat cgttgaacgt cgagttctgc
     1321 tcggggtcca gcacctcgag gagggctgcc gcgggatcgc cgcggaagtc cgaacccatc
     1381 ttgtcgatct cgtccaagag gaacagcggg ttgacgcgct ttgccttgcg catcgactgg
     1441 atgaccttgc cgggcatcga gccgatgtag gtgcgccgat ggccgcgaat ctcggcctcg
     1501 tcgcgcacgc cgccaaggct catgcgcacg aactcgcgcc ccgtggcgtt cgccatcgac
     1561 ttgccaagcg aggtcttgcc cacgccggga ggaccgacga ggcacaggat cgggcctttc
     1621 agcttattgg tgcgtgcctg caccgcgagg tactcgacga tgcgctcctt gaccttgtcg
     1681 agcccgtaat gctcggcatc gagcgtctgc tgcgcctcgt ccaagtcctt cttgaccttg
     1741 cccttgacgc cccacggaat cgagagcagc cagtcgagat agttgcgcac gacggtcgct
     1801 tccgccgaca tcgggctcat ctgcttgagc ttcttcagct cggccagcgc cttgtcgcgc
     1861 gcttctttcg agagcttggt gttctcgatc ttctcctcga actcggcgat gtcatcgcgc
     1921 tcgtcggagt cgccgagctc cttctggatc gccttcatct gctcgttcaa gtagtactcg
     1981 cgctgcgtct tctccatctg gcgcttcacg cgcgagcgga tcttgcgctc gacctgcagc
     2041 accgagatct cgctctccat gagcgtgtag acgcgctcga gccgctcgga gatcgtcgcg
     2101 gtctcgagga tctcctgctt gtcggcgatc ttgatcgcga ggtgcgaggc aatcgtgtcg
     2161 gcgagcttcg agtagtcctc gatctgcgcg acgttgccca ggacctcagg cgagatcttc
     2221 ttgttgagct tcacgtagct ctcgaactgg gtgacggccg agcgcgcgag cgcctcgatc
     2281 tcgtccttgg cgcccgtctc ctcgggcacc tgctccacct cggcctcgaa gtagtccggg
     2341 ttctcggtgt agcgggcgat gcgcgcgcgc gaggtgccct cgaccagcac cttgacggtg
     2401 ccgtcgggca gcttcaggag ctgcagcacg gaggcgagcg tgccgacggg atagatggcg
     2461 tcggtcgccg gatcgtcgtc gccggcgttc ttctgcgcgg caagcaggat gtgcttgtcg
     2521 gagcgcatca cctcttcgag cgcgttgatc gacttgtcgc gcccgacgaa gagcggaacg
     2581 atcatgtacg ggaacaccac gatgtcgcgc agcggcagaa ccgggtagag ccccttgcca
     2641 gcggccttat ctttcgtctg cttctcgtcg cccatgtcgt atccactccg gtgtggccgc
     2701 ttgcgatcgt ctgcccgccg cccg
//
LOCUS       DASVII010000150         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_115131,
            whole genome shotgun sequence.
ACCESSION   DASVII010000150 DASVII010000000
VERSION     DASVII010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115131"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1237)
                     /gene="mraY"
                     /locus_tag="VF309_01945"
                     /pseudo
     CDS             complement(146..1237)
                     /gene="mraY"
                     /locus_tag="VF309_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
     gene            complement(1227..>1841)
                     /locus_tag="VF309_01950"
     CDS             complement(1227..>1841)
                     /locus_tag="VF309_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX7465373.1"
                     /translation="AMVRASVTDRDVRFVTPRGEFTVKLQVRGEHNVRNAMAACAAAF
                     ALGIPFTAMRAGLAGFGGVPGRQQRRRAPGGSLVIDDSYNANPESMKAATQVLAQEQG
                     RKVLVMGDMGELGDEAAAMHAEVGAFARDAGIDALMALGEASRHAVQAFGKGAAHFDD
                     IDALTKAAQREAAGGAAILVKGSRFMRMERVADALAGKGDGHAV"
BASE COUNT          323 a          635 c          616 g          267 t
ORIGIN      
        1 gtatcggcag cacgcacgcg cgcgccacgc gccgcgagcc aacgaacgct cgaaagcccc
       61 gtgtcaccca ggccgagaac caggacattc ttcccttgcc attcattcgt cacctcgttc
      121 ggttctggtt gaaaggattc aaggatcagc gcagcttcaa ggtcgaaagg ccgaagagga
      181 ccagaatcat cgtgatgatc cagaagcgca ccacgacctg gttctccttc cagcccttca
      241 attcgtagtg gtggtgcagc ggcgccatgc ggaagatgcg cttgccggtc agcttgaagg
      301 aggcgacctg gatcatcacg gagagggtct cgaccacgaa cacgccgccc atgatgatga
      361 gcaccacctc ctggcggacg atgagcgcga tcatcccgat cgccgcgccc agggcgagcg
      421 cgccgacgtc gcccatgaac acctcggccg gataggcgtt gaaccagagg aaggcgaggc
      481 ccgccccgga gatcgcgccg cagatgatgg cgagctcgcc cacgcccgcg atgtacggaa
      541 acgcgaggta gcgcgcgaac accacgttgc cggagacgta cgcgaacacg gcgagcgcgg
      601 aggcgatcat caccgtgggc atgatggcga ggccgtcgag gccgtcggtg aggttcaccg
      661 cgttggagaa tcccaccacc atgaagtagc cgatgacgat gaagcccacg gcgcccagcg
      721 gaatcgccac cgtcttgaag aagggcagga tcagctcggt ctgctgcggc agcttcgcgc
      781 tgtaggcgag gaaggagatc gccgcgatgc cgatcaccga ctgccaggcc atcttggcct
      841 tcgccgagag gcccttcgga ttgcggtgca cgaccttgcg ccagtcgtcc acccagccca
      901 cggcgccgaa gccgagcgtg gtgacgagca cgatccacac gtagcggttg gcgagatccg
      961 cccagaggag cgtcgtcacc gcgaccgaga ccaggatcag cgcgccgccc atggtcggcg
     1021 tgccgctctt cacgaggtgc gtctgcgggc cgtcgtcgcg caccgcctgg ccgatcttgt
     1081 aggcggtgag cttgcggatc atcgcgggcc cgacgacgaa ctagatggcg agcgcggtca
     1141 acgcggcgag caccacgcgc agcgtgatgt aggtgaagac gttgaaggcg cgcagcttcg
     1201 catccaccga cagccactgc gcgagctcat acagcatggc cgtccccctt gcccgcgagc
     1261 gcgtcggcga cgcgttccat gcgcatgaag cgcgagccct tgaccaggat cgcggcgccg
     1321 cccgcggcct cgcgctgtgc ggccttggtg agcgcgtcga tgtcgtcgaa atgcgctgcg
     1381 cccttgccga aggcctgcac cgcgtggcgg ctcgcctcgc cgagcgccat cagcgcgtcg
     1441 atgcccgcgt cgcgcgcgaa ggcgccgacc tccgcgtgca tcgcggccgc ctcgtcgccc
     1501 agctcgccca tgtcgcccat caccaggacc ttgcggcctt gctcctgcgc gaggacctgc
     1561 gtcgcggcct tcatcgactc gggattggcg ttgtagctgt cgtcgatcac cagcgagccg
     1621 ccgggcgcgc gccggcgctg ctggcggccg gggacgccgc cgaagcccgc gagccccgcg
     1681 cgcatcgcgg tgaacggaat gccgagcgcg aaggcggcgg cgcatgcggc catggcgttg
     1741 cgcacgttgt gctcgccgcg cacctgcagc ttcaccgtga actcgccgcg cggcgtgacg
     1801 aagcgcacgt cgcgatcggt caccgaagcg cgcaccatgg c
//
LOCUS       DASVII010000151         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_330983,
            whole genome shotgun sequence.
ACCESSION   DASVII010000151 DASVII010000000
VERSION     DASVII010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330983"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF309_01955"
     CDS             complement(<1..720)
                     /locus_tag="VF309_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7465374.1"
                     /translation="MAVLTYPGSPFRLHQPFSPAGDQPQAIEKLVDGINDGLAYQTLL
                     GVTGSGKTFTMANVIAQTGRPTLVIAPNKTLAAQLYSEFREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLYIEKDSSINEHIEQMRLSATKALLERPDSIIVATVSAIYGIGDPSDY
                     HSMILHLRENEKITQREAIKRLTDMQYERNDVEFKRGTFRVRGDVLDVFPAENSETAI
                     RVSLFDDEVENLSVFDPLTGHI"
     gene            825..2042
                     /locus_tag="VF309_01960"
     CDS             825..2042
                     /locus_tag="VF309_01960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7465375.1"
                     /translation="MSLVANRLKVIKPSPTLAVSAKAAKLKPEGKDVIAMGAGEPDFD
                     TPDFIKEAAIAAIRKGLTKYTAAGGTPSLKKAIIAKYKRENDLDYTEKQILVSVGGKQ
                     TSFNLCMALLNSGDEAIVPAPFWVSYPDMAMMADGKPVFIPADIDQGFKINAQQLERA
                     ITPRSRLIWINSPSNPTGAVYTADELKSLGEVLRKHPNIIIATDDMYEHILLDGSKFV
                     NIVNVCPDLYPRTVVMNGVSKAYSMTGWRIGYCGGPQELIEAMENVQSHSTSNPTSIS
                     QYAAEAALNGDQSCIDPMVAAFKERAKVITDGLNRIPGVKCLMPTGAFYSFPDCREAI
                     RKLHAAGKIAAPTDLALCDYLLAQEKAVAAVPGSAFGAEGYLRISFATSMENIKKAVE
                     RMASAMAEAPVTA"
     gene            complement(2032..>2351)
                     /locus_tag="VF309_01965"
     CDS             complement(2032..>2351)
                     /locus_tag="VF309_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEX7465376.1"
                     /translation="ALDENAARYRGVVLGAFQQVEDNLALLNHYRTAAQAERAAVDAA
                     QRSLDFATSRYRDGAASYLEVVTSQAAALQAQRDALDLDTRQRRASVQLVRALGGGWS
                     ATP"
BASE COUNT          431 a          757 c          745 g          418 t
ORIGIN      
        1 aatatgcccc gtcagcggat cgaacaccga aaggttctcg acctcgtcgt caaagaggct
       61 cacgcgtatc gcggtttccg agttttccgc gggaaacacg tccagcacgt cgccgcgcac
      121 gcgaaaagta ccgcgcttga attcgacgtc gttgcgctcg tactgcatgt cggtcagccg
      181 cttgatcgcc tcgcgctggg tgattttttc gttctcgcgc aggtgcagga tcatgctgtg
      241 gtagtcggaa ggatcgccga tgccgtaaat cgccgacacg gtggcgacga tgatcgaatc
      301 cgggcgctcg agcaatgcct tggtcgccga caagcgcatc tgctcgatgt gctcgttgat
      361 cgacgagtcc ttctcgatgt agaggtcgcg cgagggcacg taggcctcgg gctggtagta
      421 gtcgtagtac gagacgaagt actcgaccgc gttctccggg aagaactcgc ggaactccga
      481 gtagagctgg gcggcgaggg tcttgttggg cgcgatgacc agcgtcggcc gcccggtctg
      541 cgcgatcacg ttcgccatcg tgaacgtctt tccggagccc gtgacgccga ggagcgtctg
      601 gtaggcgagg ccgtcgttga ttccgtccac cagcttttcg atcgcctggg gctggtctcc
      661 cgcgggggaa aacggctggt gcagccggaa ggggctgccg ggataggtca ggaccgccat
      721 ggggttcggg gctttcggag cgaaacagac gattttatca attccagaag gtaaaatagg
      781 aattggtcca tcctttttcc accgttcgtt ccccggagaa gtccatgtcg ctcgtcgcaa
      841 accgcctcaa ggtcatcaag ccctcgccca ccctcgccgt ctcggccaag gcggccaagc
      901 tcaagccgga aggcaaggac gtgatcgcca tgggcgcggg cgagccggac ttcgacacgc
      961 cggatttcat caaggaagcg gcgatcgcgg cgatccgcaa gggcctcacc aagtacaccg
     1021 cggccggcgg gaccccgtcc ctgaagaagg cgatcatcgc gaagtacaag cgcgagaacg
     1081 acctcgacta caccgagaag cagatcctcg tctcggtcgg cggcaagcag acttcgttca
     1141 acctctgcat ggcgctcctc aattccggcg acgaggcgat cgtgcccgcc cccttctggg
     1201 tctcgtaccc cgacatggcg atgatggcgg acggcaagcc cgtcttcatt ccggcggaca
     1261 tcgaccaggg attcaagatt aacgcgcagc agctggagcg tgcgatcacg ccgcgctcac
     1321 gccttatctg gatcaacagc ccttcgaatc cgacgggcgc ggtctacacg gccgacgagc
     1381 tgaagtcgct cggcgaggtg cttcgcaagc acccgaacat catcatcgcg accgacgaca
     1441 tgtacgagca catcctgctc gacggctcga agttcgtgaa catcgtgaac gtgtgtcccg
     1501 acctctatcc gcgcaccgtc gtgatgaacg gcgtctcgaa ggcgtactcc atgaccggct
     1561 ggcgcatcgg ctattgcggc ggcccgcagg agctcatcga ggcgatggag aacgtgcagt
     1621 cccacagcac ctcgaacccg acttccatct cgcagtacgc cgccgaagcc gcgttgaacg
     1681 gggaccagtc ttgcatcgat ccgatggtcg ccgccttcaa ggagcgcgcc aaagtaatta
     1741 ccgatggctt gaaccgcatc cccggcgtga agtgcctgat gcccaccggg gcgttctact
     1801 cgtttccgga ttgccgcgaa gcgatcagga agctgcacgc cgcgggcaag atcgcggcgc
     1861 cgacggatct cgcgctctgc gactatctgc tcgcgcagga gaaagcggtc gccgcggttc
     1921 cgggctccgc cttcggcgcg gagggctatc tcaggatctc gttcgcgacc tcgatggaga
     1981 acatcaagaa agcggtcgag cggatggcaa gcgcgatggc cgaggctccg gttacggcgt
     2041 agccgaccac cccccgccca gcgcgcgcac gagctgcacg ctcgcgcgcc gctggcgcgt
     2101 gtcgagatcc agcgcgtcgc gctgcgcctg cagcgcggcg gcctgcgacg tcaccacttc
     2161 caggtagctc gcggctccat cgcggtagcg gctggtcgcg aaatcgagcg agcgctgcgc
     2221 cgcgtccacg gcggccctct ccgcctgcgc cgccgttcgg taatggttca ggagcgcgag
     2281 gttgtcctcg acctgctgaa atgcgccgag taccacaccg cggtagcgcg cggcgttctc
     2341 gtccagcgcc g
//
LOCUS       DASVII010000152         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_438960,
            whole genome shotgun sequence.
ACCESSION   DASVII010000152 DASVII010000000
VERSION     DASVII010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438960"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VF309_01970"
     CDS             <1..334
                     /locus_tag="VF309_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX7465377.1"
                     /translation="FSFRGPGFEIESVVTRAQFEGWIAGELEAIEAALEQVLVNSSIA
                     DGDVDRVFLTGGSSFVPAVRRIFVKRFAAERVEPGNEFVSIANGLATIGLREDIDSWV
                     VTPETAKK"
     gene            331..1692
                     /locus_tag="VF309_01975"
     CDS             331..1692
                     /locus_tag="VF309_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX7465378.1"
                     /translation="MSAPIIAEELAAAVARVRATPLPDRVRKAVSMMLVDIGGLAVAA
                     RNAPYVRAAIDSLEANGGSTAIGHARALDAAGAAFVNGTAAHGEDFDDTFEGGPVHAG
                     AVIIPALLAAAERHGIEGADMFFGMAVGAEVMCRASLVAPKRIHNAGFHPTAVLGAMG
                     AAAGVAAALRVDEKTFASAQGIAGSMASGIIEYLAEGASTKRMHPGWAAQSGLRAVEL
                     ARAGFEGPRTVWEGEHGLLHGFANTTGGSWERLTGDFGDRWVVRTIAFKPYACGTMIH
                     PYIDCARRLSRQGVETDEVTRIVCTTAEGIVHRLWEPLATKRSPPNAYAAKFSMPFCI
                     AYGILHGTVGLEAFTEENAHDPQTVALAKKVSYEVDPDDPYPEEFTGHVRIHMRDGRV
                     LEERQPHLRGGRSEPLTPADIEEKFRANCAYGGWDAPRIEAWLAFAANAFAGPVDLAP
                     FRG"
     gene            1697..2449
                     /locus_tag="VF309_01980"
     CDS             1697..2449
                     /locus_tag="VF309_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7465379.1"
                     /translation="MNAGNELAGRVALVTGASRNIGRAIALALADAGAAVVVNAKSSQ
                     EEAREVVKAIEAAGGKAFASLADVADECSVGAMVKSALDRYGRLDILVNNAAVRDVAP
                     IDEIDYAAWKRITGIILDGAFLVTKASLAALRKSDAAAIVNIGGMSGHTGAAGRPHVV
                     AAKLGLVGLTRALAHDLAPDGITVNCVVPGLIATRRGASSGSKTAHLHEPLLGRRGRV
                     DEVAAAVRFIAGPNARYITGQELHLNGGAYLG"
BASE COUNT          383 a          851 c          915 g          354 t
ORIGIN      
        1 gttctcgttc cgcggcccgg gcttcgagat cgagagcgtc gtcacgcgcg cgcagttcga
       61 gggctggatc gccggcgagc tcgaggcgat cgaggcggcg ctcgagcagg tcctcgtcaa
      121 ttcgtcgatc gccgatggcg acgtggaccg cgtgttcctc accggcggca gctccttcgt
      181 gccggcggtg cgccgcatct tcgtgaagcg cttcgccgcc gagcgcgtcg agccgggcaa
      241 cgagttcgtc tcgatcgcca acgggctcgc gacgatcggg cttcgcgaag acatcgattc
      301 gtgggtggtc acgccggaga ctgcgaaaaa atgagcgcac cgatcatcgc cgaggagctt
      361 gcagccgccg tcgcgcgcgt gcgcgcaacg ccgcttccgg acagggttcg caaggccgtg
      421 tcgatgatgc tggtcgacat cgggggcctc gcggtcgccg cgcgaaacgc gccctacgtc
      481 cgcgccgcca tcgacagcct cgaggcgaac ggcggatcca ccgccatcgg ccacgcgcgt
      541 gccctcgacg ccgcgggcgc ggcgttcgtg aacggcaccg cggcccacgg cgaggatttc
      601 gacgacacgt tcgagggcgg gccggtgcac gcgggcgccg tgatcatccc cgcgctcctc
      661 gccgcggcgg agcgccacgg gatcgagggc gccgacatgt tcttcgggat ggcggtgggc
      721 gccgaggtga tgtgccgcgc gagcctcgtc gcgccgaagc gcatccacaa cgcgggcttc
      781 catcccacgg cggtgctggg cgcgatgggc gcggccgcgg gagtcgccgc cgcgctgcgg
      841 gtcgacgaga agacgttcgc ctcggcgcaa ggcatcgccg gatcgatggc gagcggcatc
      901 atcgaatatc tcgccgaagg cgcctcgacg aagcggatgc atccgggctg ggccgcgcaa
      961 tcggggctgc gggcggtcga actcgcgcgc gcgggattcg agggcccgcg aacggtgtgg
     1021 gagggcgagc acggcctcct gcacggcttc gccaacacca cgggcggctc gtgggagcgc
     1081 ctcaccggcg acttcggcga tcgctgggtg gtgcgcacca tcgcgttcaa gccctacgcg
     1141 tgcggcacga tgatccatcc gtacatcgat tgcgcgcggc gcctgtcgcg ccagggcgtg
     1201 gagaccgacg aagtcacgcg catcgtctgc acgaccgccg agggcatcgt gcaccggctg
     1261 tgggagcccc tcgcgacgaa gcgctcgccg cccaacgcgt acgccgcgaa gttcagcatg
     1321 cccttctgca tcgcgtacgg gatccttcac ggcaccgtgg gcctcgaggc cttcaccgag
     1381 gagaacgccc atgatcccca gaccgtggcg ctcgcgaaaa aggtgagcta cgaggtcgat
     1441 cccgatgatc cctatcccga ggagttcacg ggccacgtgc gcatccacat gagggacggg
     1501 cgcgttctcg aggagcggca gccgcatttg cgcggagggc gcagcgagcc gctcacgccc
     1561 gccgacatcg aggagaaatt ccgcgccaac tgcgcctacg gcggctggga tgcgccgcgg
     1621 atcgaggcct ggctcgcgtt cgcggcgaac gccttcgcgg gacccgtgga cctcgcgcca
     1681 ttcaggggat gaggacatga acgcaggcaa tgagctcgcg ggccgcgtgg cgctcgtgac
     1741 cggcgcgagc cgcaacatcg ggcgcgcgat cgcgctcgcg ctcgcggacg ccggcgccgc
     1801 cgtcgtcgtg aacgcgaagt cgtcgcagga agaagcgcgc gaggtggtga aggcgatcga
     1861 ggcggcgggc ggcaaggcct tcgcgagcct cgccgacgtg gcggacgagt gctccgtggg
     1921 cgcgatggtg aaatcggcgc tcgaccgcta cggccggctc gacatcctcg tgaacaacgc
     1981 cgcggtgcgc gacgtggcgc cgatcgacga gatcgactat gcggcgtgga agcgcatcac
     2041 gggaatcatt ctcgacggcg cattcctcgt caccaaggcg agcctcgcgg cgctgcgcaa
     2101 gagcgatgcg gccgccatcg tgaacatcgg cggcatgtcg ggccacaccg gggccgccgg
     2161 ccgcccccat gtcgtcgcgg cgaagctggg gctcgtaggc ctcacgcggg cgctcgcgca
     2221 cgacctcgcg cccgacggga tcacggtcaa ttgcgtggtc ccgggattga tcgcgacgcg
     2281 gcgcggcgcc tcgagcggat cgaagaccgc gcacctgcac gagcccttgc tcggacggcg
     2341 cggccgcgtc gatgaagtgg cggccgccgt gcgcttcatc gcgggcccga acgcgcgcta
     2401 catcacgggc caggagctgc atctgaacgg gggcgcgtat ttgggttagc ccgtcgccgc
     2461 gcgtcgacgg cgatattcgg agtggtggac cgcttcaatt cgc
//
LOCUS       DASVII010000153         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_331251,
            whole genome shotgun sequence.
ACCESSION   DASVII010000153 DASVII010000000
VERSION     DASVII010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331251"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF309_01985"
     CDS             complement(<1..294)
                     /locus_tag="VF309_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465380.1"
                     /translation="MLALTSAASAAPTVTFKAKAVPIPGFPHTGNILGAGAAIESEFT
                     IKGNEYGGFPPPLIGVTVFLPAGVKLHKAGFPNCPQAFIEAKEPEKCPKKSSAG"
     gene            complement(489..1613)
                     /locus_tag="VF309_01990"
     CDS             complement(489..1613)
                     /locus_tag="VF309_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7465381.1"
                     /translation="MTDQGATYRAAGVDYDALDAGKRLAMAKALSTSSLLGARGGRAL
                     DASRGEPAFVFELGEQTFAFVVEGLGTKSIVARHVLEAQGIDRFADVAYDTVAAILND
                     LCCVGALPLVVNAYFATGASEWYEQLERSASLLEGWRSACADAGCVWGGGESPSLPGL
                     LASEDIELAGAAVGAVPAGHRAILGEDLGPGDEIVLVASSGLHANGASLARLVAGRLS
                     DGYATPLADGTTFGEALLAPSVMYVGLVAALLEQEAPLTYISHVTGHGLLKLMRPPRP
                     LTYRIERLPDVPPVLDFLVEQAGLDTAAAYSTFNMGSGFALYCPAGEGPRIVAVAEGL
                     GLRAMVAGRVEEGPRQVLLEPVGVRFDGAQLELSANGSNA"
     gene            1676..>1990
                     /locus_tag="VF309_01995"
     CDS             1676..>1990
                     /locus_tag="VF309_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7465382.1"
                     /translation="MDFEPSQRCTEYKERLSAFMDEHIYPAEAVYERQLRESGDPHHQ
                     PAIMEELKVRAREAGLWNMFLPDADDGAGLSNADYAPLAEILGRSHIASEVCNCSAPD
                     TGN"
BASE COUNT          339 a          761 c          599 g          291 t
ORIGIN      
        1 gcccgcggag gacttcttcg ggcacttttc gggttccttc gcttcgatga acgcctgagg
       61 acagttcggg aagccggctt tgtgcagctt cacgcccgcg ggcaggaaga ccgtcacgcc
      121 gatcagcggc ggcggaaacc cgccgtattc gttgcccttg atcgtgaatt cgctttcgat
      181 cgccgcgccg gcgccgagaa tgttgccggt gtgcgggaag ccgggaatcg gcacggcctt
      241 agctttgaac gtgaccgtcg gcgccgccga tgccgcgctt gttagcgcca acatggccag
      301 gcctagaacg gcaacacagg tggatgctct gcgcatcgga ctctctccct cttgtgattg
      361 acggacccaa ccttatccgg cgcaagcagc gcgttcaagt ttgaaccatc cggaacggtt
      421 gagattttct cctgcatacg tcctctaaac ctcgaatcgc gccagagtcg cgaactctaa
      481 ggctctactc aagcgttaga tccattcgcg gacagctcca gctgtgcgcc atcgaagcgc
      541 acgccgaccg gctcgagcag cacctggcgc ggaccctcct ccacgcgccc cgcgaccatc
      601 gcccgcagcc cgagcccctc ggccaccgcc acgatccgcg ggccctcacc cgccgggcag
      661 tacagggcga aacccgagcc catattgaaa gtcgaatacg ccgccgccgt gtccaacccc
      721 gcctgctcga cgaggaagtc gagcaccggc ggcacgtccg gcaggcgctc gatccggtac
      781 gtcagcggcc gcggtgggcg catcagcttg agcaggccat gaccggtcac gtggctgatg
      841 tacgtcagcg gcgcctcctg ctccagcagc gccgccacca accccacgta catcaccgac
      901 ggcgccagca gtgcctcgcc gaacgtcgtg ccgtccgcca acggtgtcgc gtagccgtcg
      961 ctcaacctcc ccgcgactag gcgcgcgagc gacgcgccgt tggcgtgcag cccactcgag
     1021 gcgacgagca cgatctcatc gcccggcccc agatcctcgc cgaggatcgc cctgtgcccc
     1081 gcgggcaccg cgcccaccgc cgcgcccgcc agctcgatgt cctcggaggc gagcaacccc
     1141 ggtagcgatg gcgactcgcc tcccccccac acgcagccgg cgtccgcgca cgcgctgcgc
     1201 cacccctcca gcagcgaggc ggagcgctcg agctgctcat accactccga ggcgcccgtc
     1261 gcgaagtacg cgttcaccac cagcggcagc gctcccacgc agcagaggtc gttgaggatc
     1321 gccgccaccg tgtcatacgc cacgtccgcg aagcggtcga ttccctgcgc ttcgagcacg
     1381 tggcgcgcca cgatcgactt cgtgcccagg ccctccacca cgaacgcgaa cgtctgctcg
     1441 ccgagctcga acacgaacgc cggctcaccc cgcgacgcgt ccagcgcgcg gccacctctg
     1501 gcgccgagca gcgaggaggt cgacagcgcc ttcgccatcg ccaggcgctt gcccgcgtcg
     1561 agcgcgtcgt agtccacacc cgccgcgcga taggtggcgc cctgatcggt catgtggcgc
     1621 cctgatcggt catccgcgat gatcctaagc cactgccgcc gccgagggag gaccgatgga
     1681 cttcgaaccc agccagcgct gcaccgaata caaggagcgc ctcagcgctt tcatggacga
     1741 gcacatctac cccgccgagg cggtctacga gcgccagctg cgcgagtccg gcgacccgca
     1801 ccaccagccg gcgatcatgg aggagctcaa ggttcgcgcc cgcgaggcgg ggctgtggaa
     1861 catgttcctg ccagacgccg acgacggcgc cggactctcg aacgccgact acgccccgct
     1921 cgcggagatc ctcggccgct cgcacatcgc ctccgaggtc tgcaactgct ccgcgcccga
     1981 caccggcaac
//
LOCUS       DASVII010000154         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_474960,
            whole genome shotgun sequence.
ACCESSION   DASVII010000154 DASVII010000000
VERSION     DASVII010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474960"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..659
                     /locus_tag="VF309_02000"
     CDS             291..659
                     /locus_tag="VF309_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465383.1"
                     /translation="MRIFFFLAIAAVLAALPNMAGAADTKPALELRPKAALMAAVPSN
                     SAPFVAPASGPELRYAPPRDSAPAQSRSSCNADRDLCYDANSGHIVFKPARQFMPDLP
                     GMTPENISVKRDRIVFRYSF"
     gene            769..1404
                     /locus_tag="VF309_02005"
     CDS             769..1404
                     /locus_tag="VF309_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7465384.1"
                     /translation="MIRLLIVDDHDVVRMGLKQVFSGRVDMEVAGEARTGEEAIAQVR
                     ANPFDVALVDLSLSDMSGVDVLNRSKAIRPEMAVLIVSGYPEDQYAINLLKAGASGFV
                     AKDAPSENLVSAVVAAAQGRRYVSPRIADKLAQGLAGPAADQPSHTQLSEREFQIFCK
                     LASGQSVSEIAKELFLSVKTVSTYRSRILEKMNFKSNADITYYAVKNQLIA"
BASE COUNT          289 a          493 c          493 g          252 t
ORIGIN      
        1 gggatcggac ggcgcggcga aggccggcgg cgagacgatc gcgagcgcta taaacaagtt
       61 gcggaaggtg tggttcatcg cttctcccaa gtggttgcga ggcacaaggc ccatgcagac
      121 cacgataggg acatcgacgc ggccgcgaaa tgagcgcatg ccgcgagctt cggtggggtt
      181 tgtcctgcgg gaggtgggac gcgtcctaca ccgcgtgctg cggtatcggg acagcgcgat
      241 tcggccgccg cctgcatctg cgcgcgggac ggcggcgcac gatggtttcc atgagaattt
      301 ttttcttcct cgccatcgct gccgtcctcg ccgccctacc gaacatggcg ggcgccgcgg
      361 acaccaagcc cgcgctggag ctcaggccga aggccgcgct gatggccgcc gtgccgagca
      421 actcggcgcc cttcgtcgcg cccgcgtccg gtcccgagct gcgctacgcg ccgccacgcg
      481 acagcgcgcc ggcgcagagc cgatcatcgt gcaacgccga tcgcgacctg tgctacgacg
      541 ccaactcggg ccacatcgtc ttcaagccgg cccggcagtt catgccggac cttcccggca
      601 tgacgccgga aaacatttca gtgaagaggg atcgaatcgt cttccgctac tcgttctgac
      661 ggtgagagta ggatttgtcc tacgtgcaaa tccgtcctta accacgggcc cagcccgggg
      721 cagcatgctg taataagtac ctgaacccaa tcgccaggaa aacgaacaat gatccggcta
      781 ctgatcgtcg atgaccacga tgtcgtgcgg atggggctga agcaggtctt ctccggacgc
      841 gtcgacatgg aggtcgcggg cgaagcgcgt actggcgaag aggccatcgc gcaggttcgc
      901 gccaatcctt tcgatgtcgc actggtggac ctttccctct cggatatgtc gggtgtcgac
      961 gtgctgaacc gaagcaaggc gatccgtccg gagatggcgg tgctcatcgt gagcggctat
     1021 cccgaggacc agtacgcgat caacctgctg aaggcgggtg cctcgggctt cgtcgcgaag
     1081 gacgcgccgt cggaaaacct ggtctcggcg gtagtcgccg cggcgcaggg ccggcgctac
     1141 gtgagcccgc gcatcgccga caagctcgcg cagggcctcg cgggcccggc ggccgaccag
     1201 ccttcgcata cgcagctttc cgagcgcgag ttccagatct tctgcaagct cgcttccggg
     1261 cagagcgtgt ccgagatcgc caaggagctc ttcctcagcg tgaagaccgt gagcacctat
     1321 cgctcgcgca tcctcgagaa gatgaacttc aagtcgaacg cggacatcac atattacgca
     1381 gtgaaaaacc agctcatcgc ctgagagtga aatgcgggat cgacaatcca cggccgaaag
     1441 cacccggcaa cgagcaagcg cggcgcccat gccttccccg cctccccagg agagcgggcc
     1501 gcagggaagg acgcggcccc tggagtt
//
LOCUS       DASVII010000155         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_223183,
            whole genome shotgun sequence.
ACCESSION   DASVII010000155 DASVII010000000
VERSION     DASVII010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223183"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VF309_02010"
     CDS             <1..743
                     /locus_tag="VF309_02010"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7465385.1"
                     /translation="ADPATDIEGVDTAHKLTILAAIAFGIPMQFDKVYTEGITKLTAV
                     DLKYAEGLGYRIKLIGLTRRTGKGIELRVHPTLVPARRLIANVEGVMNAVLVKGDAVG
                     PTLFYGRGAGAEATASAVVADLVDVTRMATSDPEHRVPHLAFQPDRLSDAPILAMEDI
                     ETAYYLRLRALDRPGVLADVTRICADLSISIDAIFQKEAGEGEDQVDVVILTHRTVER
                     NINAAIRRIEALSTIPAPVVRVRLEELL"
     gene            781..>2072
                     /gene="thrC"
                     /locus_tag="VF309_02015"
     CDS             781..>2072
                     /gene="thrC"
                     /locus_tag="VF309_02015"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7465386.1"
                     /translation="MKYVSTRGHAASQSFTDILLEGLAPDGGLMVPEQWPRIMPVKFQ
                     AMRDLAYPQLAFEILRFFAADYPGAELKQAAFKTYTAEAFGAPEITPLRTLEPNLHVL
                     GLSNGPTLAFKDIAMQLLGNLFEQVLERQDRTLNILGATSGDTGSAAEHALKGKARLN
                     VFMLSPHGRMSPFQRAQMYSLADANVFNIAIEGMFDDCQDIVKQVSSDLAFKEAHRLG
                     AVNSINWARVVAQVVYYFKGYFAATRSNDEQVSFAVPSGNFGNIFAGYVAKRIGLPIR
                     RLVLATNENDVLDEFFRTGRYRPRKAADTHATSSPSMDISKASNFERFIWDAVDGDGD
                     KVKWLWKQVEEDGGFDLSTDKAWEKIEEDSGFVSGRSTHEDRVKTIRLVKEKYGQVID
                     PHTADGVKIAMRMREPRYPMICLETALPAKFDATIVEAL"
BASE COUNT          370 a          704 c          682 g          316 t
ORIGIN      
        1 aggccgaccc ggcgaccgac atcgagggcg tcgacaccgc gcacaagctc accattctcg
       61 cggcgatcgc gttcggcatc ccgatgcagt tcgacaaggt ctacaccgag ggcatcacga
      121 agctgaccgc ggtcgacctg aagtacgccg agggcctcgg ctaccgcatc aagctgatcg
      181 ggctcacgcg gcgcacgggc aagggcatcg agctgcgcgt gcatccgacg ctcgtccccg
      241 cgcggcggct catcgcgaac gtggagggcg tgatgaacgc ggtgctggtg aagggcgacg
      301 cggtggggcc cacgctcttc tacgggcgcg gcgcgggagc ggaggcgacc gcgagcgccg
      361 tcgtcgccga cctcgtcgac gtgacgcgca tggcgacgtc ggaccccgag catcgagtcc
      421 cgcacctcgc gttccagccg gaccgcctct ccgacgcgcc gatcctcgcc atggaggaca
      481 tcgagacggc gtattacctg cggctgcgcg ccctcgaccg tcccggcgtg ctcgccgacg
      541 tgacgcgcat ctgcgccgac ctctcgatct cgatcgacgc catcttccag aaggaggcgg
      601 gcgagggcga ggaccaggtc gacgtcgtga tcctcacgca ccgcacggtc gagaggaaca
      661 tcaacgccgc gatccggcgc atcgaggcgc tttcgaccat tccggcgccc gtggtgcgcg
      721 taaggctcga ggagctgctt tgaaaggtca ttcccgcgaa ggcgggaatc gaatgacgtc
      781 atgaaatacg tcagcacccg gggccacgcg gcatcccagt ccttcaccga cattctcctc
      841 gaaggcctgg cgcccgatgg cgggctcatg gtgccggagc agtggccgcg catcatgccc
      901 gtcaagttcc aggcgatgcg cgacctcgcc tatccgcagc ttgcgttcga gatcctgcgc
      961 ttcttcgccg cggactatcc cggcgcggag ctgaagcagg cggcgttcaa gacgtacacc
     1021 gcggaggcct tcggcgcccc cgagatcacg ccgctgcgca cgctcgagcc caacctgcac
     1081 gtcctcgggc tctcgaacgg gccgacgctc gcgttcaagg acatcgcgat gcagctcctc
     1141 gggaacctct tcgagcaggt cctcgagagg caggaccgca cgctcaacat cctcggcgcc
     1201 accagcggcg acacgggctc cgcggcggag cacgcgctga agggcaaggc gcgcctgaac
     1261 gtgttcatgc tttcgccgca cgggcgcatg agcccgttcc agcgcgcgca gatgtattcg
     1321 ctcgccgacg ccaacgtctt caacatcgcg atcgagggca tgttcgacga ctgccaggac
     1381 atcgtgaagc aggtctcgtc ggacctcgcc ttcaaggagg cccatcgcct cggcgccgtg
     1441 aactcgatca actgggcgag ggtcgtggcg caggtcgtct actacttcaa gggatacttc
     1501 gccgcgacgc ggtccaacga cgagcaggtg tccttcgccg tgccctcggg aaatttcggc
     1561 aacatcttcg cgggatacgt cgccaagcgc atcggcctgc cgatccgccg gctggtgctc
     1621 gccaccaacg agaacgacgt cctcgacgag ttcttccgca cgggccgcta ccgtccccgc
     1681 aaggcggcgg acacccatgc gacgtcgagc ccgtccatgg acatctcgaa ggcgtcgaat
     1741 ttcgagcgct tcatctggga tgcggtcgac ggcgacggcg acaaggtcaa gtggctctgg
     1801 aagcaggtcg aggaggacgg cgggttcgac ctttccaccg acaaggcgtg ggagaagatc
     1861 gaggaggact cgggcttcgt ctcggggcgc agcacgcacg aggacagagt gaagacgatc
     1921 cgcctcgtga aggagaagta cgggcaggtg atcgacccgc acaccgccga tggggtgaag
     1981 atcgcgatgc gcatgcgcga gccgcgctac ccgatgatct gcctcgagac ggcgcttccc
     2041 gcgaagttcg acgcgaccat cgtcgaggcg ct
//
LOCUS       DASVII010000156         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_187153,
            whole genome shotgun sequence.
ACCESSION   DASVII010000156 DASVII010000000
VERSION     DASVII010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187153"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF309_02020"
     CDS             <1..316
                     /locus_tag="VF309_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465387.1"
                     /translation="APAHQHFGTLLVSCEHADLRALPGGVMIYQNGTARLDALPPPGV
                     PRAEVIDELREAVLENRAPLHDGAWGLATLEVCVAMLRSARESREVRLARQVALHGII
                     RA"
     gene            313..900
                     /locus_tag="VF309_02025"
     CDS             313..900
                     /locus_tag="VF309_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7465388.1"
                     /translation="MKSDSAADAKLPTREILQHWREAVPGDRLAHLVKDATRALVRAL
                     QMRLVAHGVPFGHWAFLRILWERDGLTQRELSGRAGVMEPTTFSALKAMEGIGYVKRR
                     RLPENRKNIYVFLTAKGRALKEKLVPLAEDVNRVAVAGLADEDISRTREVLLAIIDNL
                     ARDEERGADGARRVPSTRELARLVAAGTKARRSRA"
     gene            908..2146
                     /gene="hemW"
                     /locus_tag="VF309_02030"
     CDS             908..2146
                     /gene="hemW"
                     /locus_tag="VF309_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7465389.1"
                     /translation="METKPRPAAIALAELPPLSLYVHIPWCLRKCPYCDFNSHEKRDD
                     LPEDEYVDALVADLESSLPRVWGRRLHTIFIGGGTPSLFAPRSIDRLLTAVRTLIMAD
                     PGTEITMEANPGTFERERFRGFRDAGVNRLSLGVQSFDDAKLAAIGRVHGADEARRAL
                     EAALDIFPTVNTDLMYALPGQELEQALADVREAIAFGVPHVSAYHLTLEPDTNFFRFP
                     PALPDDDVAANMQEAIEDALAGEGYGHYETSAFAKPGARARHNLNYWTFGDYLGIGAG
                     AHGKISFRDRITREERSRKPLTYLRGALGGDGIRESREVAANELPFEFMLNAMRLIEG
                     FPVALFAQRTGLAITAVDKPLRAVEAAGLIERDHATIRPTPRGQRFLNDLLEPFLPGT
                     QARTRVVPINIASPGTSRPS"
BASE COUNT          345 a          799 c          758 g          318 t
ORIGIN      
        1 ggcgccggcg caccagcatt tcggcaccct gctggtgagc tgcgagcatg ccgacctgcg
       61 cgcgcttccg ggcggcgtca tgatctatca gaacggcacg gcgcggctcg acgcactgcc
      121 accgcccggc gtgccgcgcg ccgaggtgat cgacgagctg cgcgaagccg tgctcgaaaa
      181 ccgcgcgccg ctgcacgatg gcgcatgggg gctcgcgacg ctggaagtct gcgtcgcgat
      241 gctgcgctcg gcgcgcgaat cccgcgaggt gcggctcgcg cggcaggtgg cgctgcatgg
      301 gataattcgc gcgtgaaatc cgacagcgcc gccgacgcga agcttccgac ccgcgagatc
      361 ctgcagcact ggcgcgaagc ggttcccggg gatcgcctcg cccatctcgt gaaggacgcg
      421 acgcgggccc tggtgcgcgc gctgcagatg cgcctggtcg cgcacggcgt cccgttcggg
      481 cactgggcgt tcctgcgcat cctctgggag cgcgacggcc tcacgcagcg cgagctctcg
      541 gggcgggcgg gcgtcatgga gcccaccacg ttctccgcgt tgaaggcgat ggagggaatc
      601 ggctacgtga agcgccgccg gctccccgag aaccgcaaga acatctacgt gttcctcacc
      661 gcgaagggcc gggcgctgaa ggaaaagctc gtgccgctgg ccgaggatgt gaaccgcgtc
      721 gcggtggcgg gcctcgccga cgaggacatc tcaagaaccc gcgaggtcct cctcgcgatc
      781 atcgacaatc tcgcgcgcga cgaggagcgt ggcgccgacg gcgcgcgccg cgtgccctcg
      841 acgcgggagc tcgcgcggct ggtcgcggcg ggaacgaagg cgcggcgctc gcgcgcctga
      901 tcctcccgtg gaaacgaaac cccgtcccgc cgcgatcgcg ctcgcggagc tgccgccgct
      961 gtcgctctac gtgcacattc cgtggtgcct tcgcaaatgc ccgtactgcg acttcaattc
     1021 ccacgagaag cgcgacgacc ttcccgaaga cgaatacgtg gacgcgctcg tcgccgacct
     1081 cgagtccagc ctgccgcgcg tctggggacg gcgcctgcat acgatcttca tcggcggcgg
     1141 cacgccgagc ctgttcgcgc cgcgatcgat cgaccggctg ctcaccgcgg tgcgcacgct
     1201 catcatggcc gatcccggca ccgagatcac gatggaagcg aaccccggaa cgttcgagcg
     1261 cgagcgcttc cgcggcttcc gcgacgccgg cgtgaaccgc ctgtcgctcg gcgtgcagag
     1321 cttcgacgac gcgaagctcg cggcgatcgg ccgcgtccac ggcgccgacg aggcgcggcg
     1381 cgcgctcgag gcggcgctcg acatcttccc caccgtcaac acggacctca tgtacgcgct
     1441 tccgggccag gagctcgaac aggcgctggc cgacgtgcgc gaggcgatcg cgttcggcgt
     1501 tccgcacgtc tccgcctacc acctcacgct cgagcccgac acgaatttct tccgcttccc
     1561 gccggccctg cccgacgacg acgtggcggc gaacatgcag gaagccatcg aggacgcgct
     1621 cgcgggggaa ggctatggcc actacgagac gtcggctttc gcgaagcccg gcgcgcgggc
     1681 gcgccacaat ctcaactact ggaccttcgg cgactacctg gggatcggcg cgggcgccca
     1741 cggcaagatc tcgtttcgcg atcgcatcac gcgcgaggag cgcagccgca agccgctcac
     1801 ctaccttcgc ggcgcgctcg gcggcgatgg gatccgcgaa tcgcgcgaag tcgccgcgaa
     1861 cgagctgccg ttcgagttca tgctgaacgc gatgcgcctc atcgagggat ttccggtcgc
     1921 gctcttcgcg cagcgcaccg ggctcgcgat cacggccgtc gacaagccgc tgcgcgcggt
     1981 cgaggcggcg ggactcatcg agcgcgacca cgcgacgatc cggccgacgc ccaggggaca
     2041 gcgattcctc aacgacctgc tcgagccctt ccttccggga actcaggcgc ggacgcgggt
     2101 cgtccccatc aatattgctt cgccgggtac gtcccgccct tcatgaacgg cggctcgcgg
     2161 ccgacgagcg cctcgagcat cacgagctcc gcatcggtgt gattgaggaa ggggcccggc
//
LOCUS       DASVII010000157         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_79490, whole
            genome shotgun sequence.
ACCESSION   DASVII010000157 DASVII010000000
VERSION     DASVII010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79490"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VF309_02035"
     CDS             <1..1648
                     /locus_tag="VF309_02035"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7465390.1"
                     /translation="EAGYHVPALAVPGAREAVTCESLARYESVRLFVDRAVAVQPGFR
                     VTDANCASIARICRHLDGIALAIELAAARVQSLPVEQIAARLDNCFRLLTGGDKTALP
                     RQQTLRACIDWSYDLLSIPERELFRRLAVFAGGWTMESAEAVCAGGDVGRDDVMDLLA
                     MLVDKSLVFIDASSERYRLLDTVRQYAHELLENSGEGDAVRSRHLAFYEALAERTRDE
                     IVGPNQGAWMLRLDHERENLLCAHDWCGSAPDGARIGMSLARGTKLYWINRGFLGVGH
                     SLTVEALARPGAQVRDERRCRALFDAGQLAFYMGRYGEAQRYLEESLDIARELGDKAR
                     IAAALQPLGMASLGQGHLGTARSHLEEALSLARELGNKRNLAAAMNALAQLYRIEGAL
                     DEAEVLYERALALARELDDSESVAVGLLNLAMVSICRRSPDKARDMLLQAIAIAQAIG
                     SQRVGLSGVEVSAGLAALCEDWGNAARFFGAAEAHSGSTGLHRDPADEAFLSPLMAKC
                     RGALDPAAFAADEARGRALAYDEVMAQARDWLAAAQPTRR"
     gene            complement(1635..2102)
                     /locus_tag="VF309_02040"
     CDS             complement(1635..2102)
                     /locus_tag="VF309_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465391.1"
                     /translation="MKNRALLSAFTFAFAAAGCAMHSPVAEGGPGKRPSKTCDEKGVK
                     CDVVVSVASCTPDGITLDHEVLGVAKGSRDVEIRWTISTPGYDFAKKDGIKFKTGDWP
                     KEFDLPEGNRNRYKWRDRNNLDTGTRAYDYSVTVVKSDGTACATKDPTVINDA"
     gene            complement(2197..2337)
                     /locus_tag="VF309_02045"
     CDS             complement(2197..2337)
                     /locus_tag="VF309_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465392.1"
                     /translation="MKSEPTKDTVFNSLVLFAAWMVVISTCITDDIDTGTQVVRIAYR
                     AQ"
BASE COUNT          351 a          803 c          836 g          415 t
ORIGIN      
        1 cgaggcgggc tatcacgttc cggcgctcgc agtgcccggg gcgcgcgagg cggtaacctg
       61 cgagagcctg gcgcgctacg agtcggtgcg actcttcgtc gaccgcgctg tcgcggtgca
      121 gcccgggttc cgcgtcacgg acgcgaactg cgccagcatc gcgcggatct gccgccacct
      181 cgacggcatc gccctcgcga tcgagcttgc cgcggcgcgc gtccaatcgc tgcccgtgga
      241 gcagatcgcc gcccgcctgg acaactgctt ccgcctcctc acggggggcg acaagacggc
      301 gttgccacgg cagcagaccc tgcgcgcctg cattgactgg agctacgacc tgctttccat
      361 tccggagcgc gagctattcc gccgcctcgc ggtcttcgcg gggggctgga cgatggagtc
      421 cgccgaggcg gtctgcgccg gtggcgacgt gggcagggac gacgtgatgg acctccttgc
      481 gatgctcgtc gacaaatcgc tcgtgttcat cgatgcctcg agcgagcgct accgcctcct
      541 cgacaccgtg cgccaatatg cccacgagct cctggagaat tccggggaag gcgacgcggt
      601 gaggagccgg cacctggcgt tctacgaggc gctggctgag cgcacgcgcg atgagatcgt
      661 cggcccgaat caaggtgcgt ggatgctgcg cctcgaccac gagcgcgaga acctgttatg
      721 cgcgcacgat tggtgcggga gcgcacccga cggggcgcga atcggcatga gtctcgcgcg
      781 cggcaccaag ctctactgga tcaaccgcgg attcctgggt gtcggccaca gcctcacggt
      841 ggaagcgctg gcgcgcccgg gcgcgcaggt gcgcgatgaa cggcgctgcc gcgcgctctt
      901 cgatgccggc cagctcgcgt tctacatggg acgatacggc gaggcgcagc gctatctcga
      961 ggagagtctc gacattgccc gcgagctggg ggacaaggcg cgcatcgccg cggcgttgca
     1021 gccccttggc atggcgtcgc tcgggcaagg acacctgggg actgcgcgtt cgcacctgga
     1081 ggaagccctc tccctcgcgc gcgaattggg taacaagcgc aacctcgccg cggcgatgaa
     1141 cgccctcgcg cagctctacc gcatcgaggg cgccctcgac gaggccgagg tcctctacga
     1201 gagggcgctc gcgctcgccc gcgagctcga cgacagcgag agcgtggcgg tggggctcct
     1261 caacctcgcg atggtgtcga tctgcaggcg cagcccggac aaggcgcgcg acatgctgct
     1321 gcaggcgatc gcgatcgcgc aggcgatcgg ctcgcagcgc gtcgggctga gcggcgtcga
     1381 ggtctcggcg ggcctggccg cgctatgcga ggactgggga aatgccgcgc gcttcttcgg
     1441 cgccgccgag gcgcacagcg gcagcaccgg gctgcatcgc gatcccgcgg acgaggcgtt
     1501 cctctcgccc ctcatggcga aatgccgcgg cgcgctcgat ccggcggcgt tcgcggccga
     1561 cgaggcccgc gggcgcgctc tcgcctacga cgaggtgatg gcgcaggcgc gcgactggct
     1621 ggccgccgcg cagcctacgc gtcgttgatg accgtcgggt ccttcgtcgc gcacgccgtg
     1681 ccgtccgact tcacgaccgt gacgctgtaa tcgtaggcgc gcgtgcccgt gtcgaggttg
     1741 ttgcgatcgc gccatttgta cctgttcctg ttgccctcgg gcaggtcgaa ctccttcggc
     1801 cagtcgcccg tcttgaactt gatgccgtcc ttcttcgcga aatcgtagcc gggggtcgag
     1861 atcgtccatc ggatctcgac gtccctcgat cccttcgcga caccgagcac ctcgtgatcg
     1921 agggtgatgc cgtcgggcgt gcacgacgcg accgagacga cgacgtcgca tttgacccct
     1981 ttctcgtcac acgtcttgct gggcctcttt ccgggtccgc cctccgccac gggcgagtgc
     2041 atcgcgcatc cggcggcggc gaatgcgaaa gtgaatgcgg acaacagtgc gcggtttttc
     2101 attttcgagc tctcccgttc gttcttggtt tgtacagaga ggcaggcggt gcggcgggac
     2161 ctgcgatccg gcgcctatgg taatacgatg cgccggtcac tgcgcccggt aggcgatgcg
     2221 caccacctgc gtgccggtgt cgatgtcgtc ggtgatgcat gtcgagatca cgaccatcca
     2281 cgcggcgaac aggacgaggg aattgaagac cgtgtccttg gtgggttccg atttcatggc
     2341 ggttccttgc gagtcagggg aggtgactgt ctcgcaaccg ggggccgccc gtcctttagc
     2401 cgttc
//
LOCUS       DASVII010000158         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_151235,
            whole genome shotgun sequence.
ACCESSION   DASVII010000158 DASVII010000000
VERSION     DASVII010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151235"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VF309_02050"
     CDS             complement(<1..1444)
                     /locus_tag="VF309_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7465393.1"
                     /translation="MDILSGLMQGFHVALTPANLLFCVVGVVLGTVVGVLPGLGPPAT
                     IAMLLPLTFKLDPTGAMIMLAGIYYGAKYGGSTTSILLNVPGESSSVVTCIDGYQMAR
                     KGRAGPALGMAAIASFIAGTFGVVALMLVAPALAKVSLAFSAPEYFALMVLGLTLVVL
                     LAGESLTKALLAMLVGLWIASMGTDLFTATSRYTFGQMELLGGIDFTIVAIGIFAIGE
                     VLANMEPTQPAEMLPVPKGLRNLMPTWDDMKRCRFAFVNGSVIGFLIGLLPGAGATIA
                     SFMSYGIEKAVSKHPEEFGQGAVEGVAAPEGANNAETGGALVPLLTLGIPGSGTTAIL
                     LAALVLWGFKPGPLFIQDSPQLFWGLVASMYIGNVLLLILNLPLVAVFAQLLKMPGYA
                     MYPLILGVSIVGVYTVSASMFHLWLLAGFGLLGYAMRKLEFPTAPLVLGLVLGDQMEK
                     ALRQSLMMSQGDLSILLRPIPSVLLGVAALL"
     gene            complement(1447..>1765)
                     /locus_tag="VF309_02055"
     CDS             complement(1447..>1765)
                     /locus_tag="VF309_02055"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7465394.1"
                     /translation="AMIVLSLALVATTVLRGAPEPSRSISPWQDLGRALACWAAFVAS
                     VALMPVVGFPIAFAFLTWFIVAVMARQPQRTAWAVAIGGSALFYAIFEFGLDLSLPRG
                     MLY"
BASE COUNT          341 a          574 c          625 g          225 t
ORIGIN      
        1 gcagcagcgc cgcgacgccg agcagcacgc tcggaatggg gcgaagcaat atcgacaggt
       61 cgccctgcga catcatgagc gactggcgca gcgccttctc catctggtct ccgaggacca
      121 gccccagcac cagcggcgcc gtcgggaact cgagcttgcg catcgcgtag cccaacaatc
      181 cgaagcccgc gaggagccac aggtggaaca tgctcgccga aaccgtgtac acgccgacga
      241 tcgacacgcc gaggatcagc gggtacatcg catagccggg catcttgagc agctgcgcga
      301 agacggcgac gagcggcagg ttgaggatca ggaggagcac gttgccgatg tacatgctcg
      361 cgacgagccc ccagaagagc tgcgggctgt cctggatgaa gagcggcccg ggcttgaagc
      421 cccacagcac gagcgccgcg aggaggatcg cggtggtgcc gctgccggga attccgagcg
      481 tgaggagcgg caccagcgcg ccgcccgtct cggcgttgtt cgcgccctcg ggagcggcga
      541 cgccctcgac cgcgccctgg ccgaactctt cgggatgctt cgagaccgcc ttctcgatgc
      601 cgtagctcat gaacgaggcg atggtcgcgc ccgcgccggg cagcaggccg atgaggaagc
      661 cgatcacgga gccgttcacg aacgcgaacc ggcagcgctt catgtcgtcc cacgtcggca
      721 tgaggttcct gagtcccttc ggaacaggaa gcatctcggc gggctgcgtg ggctccatgt
      781 tcgcgagcac ctcgccgatg gcgaagatgc cgatggcgac gatggtgaag tcgatgccgc
      841 cgagaagctc catctgcccg aaggtgtagc gcgaggtcgc ggtgaagaga tcggttccca
      901 tgctcgcgat ccacaggccc acgagcatgg cgaggagcgc cttggtgagg gattctccgg
      961 cgaggagcac caccagcgtg aggccgagca ccatcagcgc gaaatattcg ggcgcgctga
     1021 aggcgagcga gactttcgcc agcgcgggcg ccaccagcat cagcgcgacg acgccgaagg
     1081 ttcccgcgat gaacgaggcg atcgcggcca tgccgagggc gggcccggcg cggcccttgc
     1141 gcgccatctg gtagccgtcg atgcaggtga cgaccgacga cgattcgccg ggaacgttga
     1201 gcaggatcga ggtggtcgag ccgccgtact tcgcgccgta gtagatcccc gcgagcatga
     1261 tcatcgcccc ggtgggatcg agcttgaacg tgagcggcag cagcatcgcg atggtcgccg
     1321 gcggcccgag tcccggaagc acgccgacca cggtgccgag caccacgccc acgacgcaga
     1381 agagcaggtt cgccggcgtg agcgcgacgt ggaagccctg catgagcccc gagaggatgt
     1441 ccatgcctag tagagcattc cgcggggcag cgacaggtcg agcccgaact cgaagatcgc
     1501 gtagaagagc gccgatccgc cgatcgcgac ggcccacgcg gtgcgctgcg gctggcgcgc
     1561 catcaccgcg acgatgaacc acgtgaggaa cgcgaaggcg atggggaatc cgacgacggg
     1621 catcagcgcg acgctcgcga cgaaggcggc ccagcacgcg agcgcccggc ccaggtcctg
     1681 ccatggcgag atcgaacgcg acggttcggg cgcgccgcgc agcaccgtcg tcgcgacgag
     1741 ggcgagggac agcacgatca tggcg
//
LOCUS       DASVII010000159         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_151242,
            whole genome shotgun sequence.
ACCESSION   DASVII010000159 DASVII010000000
VERSION     DASVII010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151242"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VF309_02060"
     CDS             <1..1212
                     /locus_tag="VF309_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX7465395.1"
                     /translation="DQFPDKKTAAQVAGYVSIGSDVFLRLIKMLIGPLVFSTLVVGIA
                     HMGDAASVGRVFAKALGWFVTASLVSLVLGLILANLMQPGHNLGLPLPDIGSSANLAT
                     SKFTLKDFVSHLVPKSFAEAMANNEILQIVVFSMFFGVALASLGEKAKTLVAAIDELA
                     HAMLKITGYVMKLAPIAVFCAMAATVAVNGLAILLKFAVFMGDFYVGLVLLWVVLVGA
                     GFAVLGPRVFKLLVLIKEAFMLSFATASSEAAYPKILDALDRFGVKRKISSFVMPMGY
                     SFNLDGSMMYCTFATLFIAQAYNIELSIGTQVTMLLILMLTSKGMAGVPRASLVVIAA
                     TLNQFNIPEAGLLLILGVDTFLDMGRSATNAIGNSIATAAVAKWEGELLPETAAREYA
                     QELDAAIAARA"
     gene            1222..>1585
                     /locus_tag="VF309_02065"
     CDS             1222..>1585
                     /locus_tag="VF309_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7465396.1"
                     /translation="MRFPLPCVGAWLVLAAGAALAQSEAPLELTGTLAKVRASGAVTI
                     AHRESSIPFSYLSSRGEPIGYSIELCRKIVEAMSETVGRDLEIKWLPVTSETRIEAIA
                     TGRADLECGSTTSNLERQK"
BASE COUNT          245 a          536 c          516 g          288 t
ORIGIN      
        1 gaccagttcc ccgacaagaa gacggcggcg caagtcgcgg gctacgtgtc gatcggctcg
       61 gacgtgttcc tgcgcctcat caagatgctc atcgggccgc tggtgttctc cacgctggtg
      121 gtcggcattg cgcacatggg cgacgcggcc tcggtcgggc gcgtattcgc gaaggcgctc
      181 ggatggttcg tcaccgcctc gctggtgtcg ctggtgctgg gcctcatcct cgccaacctg
      241 atgcagcccg gccataacct cggcctgccg ctgccggaca tcggctcctc ggccaatctc
      301 gccacctcga aattcacgct gaaggatttc gtgagccacc tggtgcccaa gtcgttcgcc
      361 gaggcgatgg ccaacaacga gatcctgcag atcgtcgtgt tctcgatgtt cttcggcgtg
      421 gcgctcgcct cgctcggcga gaaggcgaag acgctggtcg ccgcgatcga cgagctcgcc
      481 cacgccatgc tcaagatcac cggctacgtg atgaagctgg cacccatcgc ggtgttctgc
      541 gccatggccg ccaccgtcgc ggtgaacggg ctcgcgatcc tgctcaagtt cgcggtgttc
      601 atgggcgact tctacgtcgg cctggtgctg ctctgggtgg tcctggtcgg cgccggtttc
      661 gcggtcctgg ggccgcgggt cttcaagctg ctggtgctca tcaaggaggc cttcatgctg
      721 tcgttcgcga cggcgagctc ggaagcggcc tatccgaaga tcctcgacgc gctcgaccgc
      781 ttcggagtga agcgcaagat ctccagcttc gtgatgccga tgggctactc gttcaacctc
      841 gacgggtcga tgatgtactg caccttcgcg acgctgttca tcgcgcaggc ctacaacatc
      901 gagctgtcga tcggcaccca ggtcaccatg ctcctcatcc tcatgctcac ctccaagggc
      961 atggcgggcg tgccgcgcgc ctcgctcgtg gtgatcgccg cgacgctgaa ccagttcaac
     1021 atcccggaag cgggcctgct cctcatcctg ggcgtcgata ccttcctcga catggggcgc
     1081 tcggcgacca acgcgatcgg caactcgatc gcgacggccg cggtcgcgaa gtgggaaggc
     1141 gagctgctgc cggagacggc ggcgagggaa tacgcgcaag agctcgacgc ggccatcgcc
     1201 gctcgcgcct gacgaggccg cgtgaggttc ccattgccgt gcgtcggggc gtggctggtg
     1261 ctcgcggccg gcgccgcgct cgcccaaagc gaagccccgc tggagctcac cggcacgctc
     1321 gccaaggtac gcgcgagcgg tgcggtcacc atcgcccacc gcgaatcgtc gattccgttc
     1381 tcctaccttt cgtcgcgcgg cgagccgatc ggctactcga tcgagctgtg ccggaagatc
     1441 gtcgaggcga tgtcggagac cgtcgggcgc gacctggaga tcaagtggct gccggtcacg
     1501 tcggagacgc gcatcgaggc gatcgccacc ggccgcgccg acctcgaatg cggctccacg
     1561 accagcaatc tcgagcgcca gaagc
//
LOCUS       DASVII010000160         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_79512, whole
            genome shotgun sequence.
ACCESSION   DASVII010000160 DASVII010000000
VERSION     DASVII010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79512"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VF309_02070"
     CDS             complement(<1..1235)
                     /locus_tag="VF309_02070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061702.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7465397.1"
                     /translation="MNQEIVNLFGPQTQQQNFDQIRIAIASPEKILSWSYGEIKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRMKYKGVICEKCGVEVTLAKVRRER
                     MGHIELAAPVAHIWFLKSLPSRIGLLLDMTLKDLERVLYFENYIVLEPGLTSLKPLQL
                     LSEEDYMKAQEDFGEDSFTASIGAEAIRELLKGLDLERLAADLRVEIAESTSELKPKK
                     LAKRLKVVEAFIQSGNKPEWMILTVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLMELRAPDIIIRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLK
                     GKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYNKREERGL
                     VATIKQAKEMVELQRPEVW"
     gene            1290..>1552
                     /locus_tag="VF309_02075"
     CDS             1290..>1552
                     /locus_tag="VF309_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465398.1"
                     /translation="MAIRLRYSAAGRGSRALTGVSSGRGCTSSTFSPSARIFGPIKDY
                     ECLCGKYKRMKFRGITCEKCGVEVTLTKVRRERMGHIELASPVA"
BASE COUNT          276 a          503 c          471 g          302 t
ORIGIN      
        1 tcccacactt ccggacgctg gagctcgacc atctccttcg cctgcttgat cgtggcgacg
       61 agaccgcgtt cctcgcgctt gttgtagatg aacggcttga acagctcgag cgccatcttc
      121 ttcggcaggc cgcactgatg gagcttcagc tccggcccga ccacgatgac ggaacgtccc
      181 gagtagtcca cgcgcttgcc gagcagattc tgccggaagc ggccctgctt gcccttcagc
      241 atgtcggcaa gcgacttcag tggacgcttg ttggcgccgg tgatgacgcg tccgcgccgt
      301 ccgttgtcga acagcgcatc gaccgcctcc tgaagcattc gcttctcgtt gcggatgatg
      361 atgtccggcg cacggagctc catcagccgc ttcagacgat tgttgcggtt gatgacgcgg
      421 cgatagagat cgttcaggtc cgaggtcgcg aatcggccgc catcgagcgg caccagcgga
      481 cgaagctccg gcggaatgac cggaaccacg gtcaggatca tccactcggg cttgttgccc
      541 gactggatga acgcctcgac caccttcagg cgcttggcga gcttcttggg cttgagctca
      601 gaggtcgact cggcaatctc gacgcggaga tccgccgcca gccgctcgag gtcgagaccc
      661 ttgagcagct cgcggatcgc ctcggccccg atcgaggccg tgaacgaatc ctcgccgaaa
      721 tcctcctgcg ccttcatgta gtcctcttcg gacaggagct ggagcggctt gagcgaggtc
      781 aaccccggct cgagcacgat gtagttctcg aaatagagca cacgctccag gtccttcagc
      841 gtcatgtcga gcagaaggcc gatgcgcgag ggcagcgact tcaagaacca gatatgggcg
      901 accggcgcgg cgagctcgat atggcccatg cgctcgcgcc ggaccttggc cagcgtcacc
      961 tcgacgccgc acttctcgca gatgacgccc ttgtacttca tgcgcttgta cttgccgcac
     1021 aagcactcat agtccttcac cggaccgaag atgcgcgcgc agaacagacc gtcccgctcc
     1081 ggcttgaagg tgcggtagtt gatcgtctcc ggcttcttga tctccccata cgaccaggac
     1141 agaatctttt ccgggctcgc gatggcgatg cggatctgat cgaaattctg ctgctgggtc
     1201 tgaggaccga agagattgac gatttcctgg ttcattcttt aggtctccca ccgggcgcta
     1261 acggcgccct acggttggtc gcaatgcgtt tggcaatccg tctccgctat tcggccgccg
     1321 ggcgcggcag ccgcgccctg accggggtct cttccggccg cggctgcacc agctcgacat
     1381 tcagcccgag cgcccgcatc ttcggaccga tcaaggacta cgagtgcctg tgcggcaagt
     1441 acaagcgcat gaagttccgc ggcataacct gcgaaaagtg cggcgtcgag gtgacgctca
     1501 ccaaggtgcg gcgcgagcgc atgggccaca tcgagctggc ctcgccggtg gc
//
LOCUS       DASVII010000161         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_331433,
            whole genome shotgun sequence.
ACCESSION   DASVII010000161 DASVII010000000
VERSION     DASVII010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331433"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1311)
                     /locus_tag="VF309_02080"
     CDS             complement(163..1311)
                     /locus_tag="VF309_02080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7465399.1"
                     /translation="MAEALPAKFGRFEVIDELGRGAMGVVYRAHDSALGRTVAIKTIA
                     LTGSAAERDVHEARFLQEARAAGSIGHPAIITIYDVGREGDTAFIAMELLEGQDLREL
                     IRGVSLMPSRSVAIAAAVADGLAYAHERGVVHRDIKPGNIMVLPDSRVKIMDFGIARL
                     HEPTVKTQTGVLLGSPQYMAPEQIAGQPFDHRADIFSLGLVLYEMLTGVKPFQGEDIP
                     ELMFKVANMPATPPSHIAPDLPPVIDYIVARALKKRANERYASAGELAKDLREAAAEV
                     AAGEAAGLERADAKTAPNATPSLQGAGTASAMAREEPIELRASPRFDSTEGLARLSVL
                     PAEADATSSRAGWTVPANRERRKIDPAHAWLAVAYVVALIAAIAIVMA"
     gene            complement(1311..2201)
                     /locus_tag="VF309_02085"
     CDS             complement(1311..2201)
                     /locus_tag="VF309_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465400.1"
                     /translation="MTPAATPIHANVAFLRIPQFATLPVSEQAALKERLESRARSAIS
                     ALAADQRIVLDADDGLAIIVFGEPALALDVAQALHGSAPESPLQAGLNYGPLALTSGG
                     ADARVFGDGLTAAAAAARFASPEKLLVTHDFAKALQSRDPARAADLATAGDFTDTRVR
                     LHSFYTPDASLGSSHRRRLLAYGVGGVATILLLGLAGREAGKRLFPALPAVVRFNVRP
                     RGEIVVDGVSRGVTPPLREVEVGAGHRVVHVRNPGFPTLEVALDLKPGEHTTIAHVFA
                     RPAPPKQDLWRDFKKKFGGS"
     gene            complement(2277..>2488)
                     /locus_tag="VF309_02090"
     CDS             complement(2277..>2488)
                     /locus_tag="VF309_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX7465401.1"
                     /translation="ALSEFAGTVLCISHDRWFLDRICTHILAAEGDSQWVLYPGNYHE
                     YEEDKHERLGDEAAAPHRLRFKALK"
BASE COUNT          368 a          858 c          892 g          370 t
ORIGIN      
        1 cccgtgccga aggtgatgct gtcgccgaac gcaagcacga tcgcgccgcg atcgagcttt
       61 gcgatcttcg gcgtcgatcc gccgcacgcg cccaggacca gcgcggcggc gatcgacgcg
      121 acaagggccc tcaccgcgga gtcgacgcgc ggcacggcgc cgtcacgcca tcacgatcgc
      181 gatcgcggcg atgagcgcca cgacataggc cacggccagc cacgcgtgcg ccggatcgat
      241 cttgcgccgc tcgcgattcg cgggaacggt ccagcccgcg cgcgacgacg tggcatcggc
      301 ttccgcggga agcaccgaca agcgcgccag gccctcggtc gaatcgaagc gcggcgaggc
      361 ccgcagctcg atcggctctt cgcgggccat cgcgctcgcg gtgccggcgc cctgaagcga
      421 cggcgtggca ttcggcgcgg tcttcgcgtc cgcgcgctcg aggcccgcag cctctcccgc
      481 ggccacttcc gccgcggctt cgcgcaggtc cttcgcgagc tcgccggcgc tcgcgtagcg
      541 ctcgttcgcc ctcttcttga gcgcgcgcgc gacgatgtag tcgatgaccg gcggaaggtc
      601 gggcgcgatg tggctgggcg gggtggctgg catgttcgcg accttgaaca tgagctccgg
      661 gatgtcctcg ccctggaacg gcttcacgcc cgtgagcatc tcgtagagca ccaggcccag
      721 cgagaagatg tcggcgcgat ggtcgaaggg ctggccggcg atctgctcgg gcgccatgta
      781 ctgcggagag ccgaggagca cgcccgtctg cgtcttcacg gtgggctcgt gcagccgcgc
      841 gatgccgaaa tccatgatct tcacgcggct gtcggggagc accatgatgt tgcccggctt
      901 gatgtcgcga tgcacgacgc cgcgctcgtg cgcgtaggcg agcccgtcgg cgaccgcggc
      961 ggcgatcgcc accgagcggc tcggcatgag gctcacgccg cggatgagct cgcgcaggtc
     1021 ctgcccctcg agaagctcca tcgcgatgaa cgccgtgtcg ccttcgcggc ccacgtcgta
     1081 gatggtgatg atcgcgggat ggccgatgct tcccgcggcg cgcgcctcct gcaggaaacg
     1141 cgcctcgtgc acgtcgcgct ccgccgcgct gccggtgagc gcgatggtct tgatggcgac
     1201 ggtgcgcccg agcgccgagt cgtgggcccg gtacacgaca cccatcgcgc cgcgcccgag
     1261 ctcgtcgatc acctcgaaac gcccgaactt cgccggaagc gcttcagcca tcacgagcct
     1321 ccgaacttct tcttgaagtc gcgccacagg tcctgcttcg gcggcgcggg acgggcgaat
     1381 acgtgggcga tcgtcgtgtg ctcgccgggc ttcaggtcga gcgcgacctc gagcgtcggg
     1441 aatcccgggt tgcgcacgtg caccacgcgg tggcccgccc cgacttcgac ctcgcgcagc
     1501 ggcggcgtga ccccgcgcga gacgccgtcg acgacgatct cgccgcgcgg ccgcacgttg
     1561 aaccgaacca cggcgggcag cgccggaaag aggcgcttgc cggcttcacg cccggcgagg
     1621 cccaggagca ggatggtcgc cacgccgccc acgccatagg cgagcagccg ccggcgatgg
     1681 gacgatccga gcgacgcgtc gggcgtgtag aacgagtgca ggcgcacgcg ggtgtcggtg
     1741 aaatcgccgg ccgtcgcgag atccgcggcg cgggcgggat cgcggctttg cagcgccttg
     1801 gcgaaatcgt gcgtgacgag tagcttctcg ggcgaggcga agcgcgccgc cgccgccgcg
     1861 gcggtgagcc catcgccgaa cacgcgggcg tcggcgccgc ccgaggtgag cgccagcggg
     1921 ccgtagttga ggccggcctg caacggcgac tccggggcgc tcccgtgcag cgcctgcgcg
     1981 acgtccagcg cgagcgccgg ctcgccgaac acgatgatcg cgagcccgtc gtccgcatcc
     2041 agcacgatgc gctgatcggc ggcgagcgcg gaaatggcgg accgtgcgcg gctttcgagg
     2101 cgctccttca gcgccgcctg ctcggacacc ggcagcgttg cgaactgcgg gatgcgcagg
     2161 aacgcgacat tggcgtggat gggcgtggct gccggagtca ttgcagcagt gtaattggtg
     2221 tcaggtacga attatcccag aggagtcgct cctcgataat tcgtacctga caccaattac
     2281 ttcagcgcct tgaagcgcag gcggtggggt gcggcggcct cgtcgccgag gcgctcgtgc
     2341 ttgtcttcct cgtactcgtg gtagttgccc ggatagagca cccactgcga gtcgccctcg
     2401 gcggcgagga tgtgcgtgca gatgcggtcg aggaaccagc ggtcgtggga gatgcaaagc
     2461 accgtgcccg cgaactcgct caacgcgt
//
LOCUS       DASVII010000162         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_690929,
            whole genome shotgun sequence.
ACCESSION   DASVII010000162 DASVII010000000
VERSION     DASVII010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690929"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VF309_02095"
     CDS             <1..416
                     /locus_tag="VF309_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7465402.1"
                     /translation="GLAGLRVGYGLAHPEIAELMNRVRQPFNVNHLAMVAACAALDDG
                     EFIERSRAVNAAGLAQLRAGFERLALDFIPSFGNFITVRVGNAARIYQAMLAEGVIVR
                     PIAGYGMPEHLRVTVGLPAHNERFLAALERALARK"
     gene            421..1311
                     /locus_tag="VF309_02100"
     CDS             421..1311
                     /locus_tag="VF309_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX7465403.1"
                     /translation="MRRLKTLAVIGVGLIGGSFAMALRRAGAVTGVVGYDRDSSAMQL
                     ATELGVIDTAADSVSEAASGADLVVVAVPVRATATVLRDVGISIDAGAVITDVGSTKG
                     EVVRTARNELRERFARFVPGHPIAGRETSGVESANADLFRGARVVLTPDEATAVDAVE
                     LVRGVWEAIGGRVAVMAPEEHDRVFAAVSHLPHLLAFALVSELAAREDAAELLGFAGG
                     GFRDFTRIAASSPEMWRDIALQNRAALLEELDRYGAQLAVFRALIDKGDAPGLERLMN
                     EARASRESLKAGMRRGSPLE"
     gene            1313..>1614
                     /locus_tag="VF309_02105"
     CDS             1313..>1614
                     /locus_tag="VF309_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7465404.1"
                     /translation="MQFIDLPPATRAAGTLRLPGSKSISNRTLLLAALARGTTELCAL
                     LDSDDTRVMRAALRTLGVRIESLGSEERLLVHGLGDARAFPVKEASLSLGNAGTAFR"
BASE COUNT          221 a          565 c          584 g          244 t
ORIGIN      
        1 tcggccttgc cggactgcgc gtgggctacg gcctcgccca cccggagatc gcggagctga
       61 tgaaccgcgt gcgccagccc ttcaacgtga atcacctcgc gatggtcgcg gcatgcgcgg
      121 cgctggacga cggggagttc atcgagcgca gccgcgcggt gaatgccgcg ggcctggcac
      181 agctgcgcgc gggcttcgag cgcctcgcgc tcgacttcat cccgtccttc ggcaacttca
      241 tcaccgtgcg cgtcggcaac gccgcgagaa tttaccaggc gatgctcgcg gaaggcgtga
      301 tcgtgcggcc catcgcgggc tacggcatgc ccgagcacct gcgagtcacc gtcggattgc
      361 ccgcgcacaa cgagcgcttc ctggcggcgc tcgagcgcgc gctcgcgagg aaatgagccc
      421 atgcgcaggc tgaagacgct cgcggtcatc ggcgtcggcc tcatcggcgg atccttcgcg
      481 atggcgttgc gccgtgcggg cgccgtgacg ggcgtggtgg gttacgaccg cgactccagc
      541 gcgatgcagc ttgcaaccga gctcggcgtc atcgataccg cggccgattc cgtttccgag
      601 gccgcgagcg gcgcggacct ggtcgtggtg gcggtgccgg tgcgcgcgac cgcgacggtg
      661 ctgcgcgatg tcgggatttc catcgacgcc ggcgccgtga tcaccgacgt aggcagcacc
      721 aagggcgagg tggtgcgcac cgcgcgcaac gagcttcgcg agcgcttcgc gcgcttcgtt
      781 cccgggcatc cgatcgccgg gcgcgagacg agcggcgtgg aaagcgcgaa cgcggacctc
      841 ttccggggcg cgcgcgtggt gctcacgccc gacgaagcaa ccgccgtcga cgccgtggag
      901 ctcgtgcgcg gcgtctggga ggcgatcggc gggcgcgtcg ccgtgatggc gcccgaggag
      961 cacgaccgcg tcttcgccgc ggtgagccac ctgccgcacc tgctcgcgtt cgcgctggtc
     1021 tccgagctcg ccgcgcgcga ggacgccgcg gagctcctcg gattcgccgg cggcggattc
     1081 cgcgatttca cgcgcatcgc ggcgagctcg ccggagatgt ggcgcgacat cgcgctgcag
     1141 aaccgcgcgg ccctcctgga ggagctcgat cgctacggcg cgcagctcgc ggtcttccgc
     1201 gcgctcatcg acaagggcga cgcgcccggc ctcgagcggc tcatgaacga agcccgcgcc
     1261 agccgcgaat cgctcaaggc gggcatgcgg cgcggatccc cgctcgaata gcgtgcagtt
     1321 catcgatctt ccgccggcga cacgtgccgc gggcaccctc aggcttccgg gttccaagag
     1381 catctcgaac cggacgctcc tgctggcggc gctcgcgcgc ggcacgacgg agctgtgcgc
     1441 actccttgat tccgacgaca cgcgcgtgat gcgcgcggcg cttcgcacgc tcggcgtgcg
     1501 catcgaatcg ctgggtagcg aggagcgcct tctcgtgcac ggcctgggcg atgcgcgcgc
     1561 gttcccggtg aaggaggcaa gcctttccct cggcaatgcg gggacggcgt ttcg
//
LOCUS       DASVII010000163         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_654985,
            whole genome shotgun sequence.
ACCESSION   DASVII010000163 DASVII010000000
VERSION     DASVII010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654985"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VF309_02110"
     CDS             <1..757
                     /locus_tag="VF309_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465405.1"
                     /translation="GRSFVYNIPAIRLILEKLPATLELAVSAMVIAVVIGIPLGLVAG
                     LKPDTWLGRGIMAGSIVGFSLPTFWVGLVLIMVFSVQLGWLPSNGRGETVLLLGVPVS
                     FLTLDGLAHLALPALNLALFKLSLLVRLTRAGTREALLQDYVKFARAKGLGPGRVIGV
                     HVLKNILIPIVTVIGLELGSVIAFAIVTESIFAWPGSGKLLIDSINQLDRPVIVAYLM
                     VTVTIFIVINFVVDVLYSLLDPRVRLGGAAPAH"
     gene            763..1707
                     /locus_tag="VF309_02115"
     CDS             763..1707
                     /locus_tag="VF309_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465406.1"
                     /translation="MGIAAPEAPQTAATSAGPPSMAGVESPWRRFVSEFAESGLALAG
                     LVLLVALVLVAIAAPYISPQNPYDLSQLDVLDSRLAPGEHSSTSGKPYWLGTDDQGRD
                     MLSGIFYGLRISLAVGVASTAIALAIGLVLGLAAAYFGGWVDGVIMRIADIQLSFPAI
                     LIALILLAILGQGVGKIIAALVTIQWAYYARTVRSAALVERQKEYVEAAKVLALSQPR
                     IVFRHLLPNCLPPLIVVATVQIAHAISLEATLSFLGLGLPITEPSLGLLIANGFQYLL
                     SGKYWISFYPGLALLLTIVSINLVADQLRDVLNPRLRK"
     gene            1719..2705
                     /locus_tag="VF309_02120"
     CDS             1719..2705
                     /locus_tag="VF309_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465407.1"
                     /translation="MSHAATRSGAALLEVEGLRMHFFTKAGVVKAVDDVSFRVERGRI
                     LGLVGESGSGKSMTGYSILGLVDAPGRIVAGSIRLNGTDITRLGEPAWRKLRGERIAM
                     IFQDPMMTLNPVLRIDTQMMEAVRAHHDVSRGAARETCREALARVGIASPEERLRNYP
                     HQFSGGMRQRVAIAIALLNRPDLIIADEPTTALDVTIQGQILFEMQKLCRETGAGLIW
                     ITHDLSVISGLADEVCVMYAGRIVESGTVDEILDRPLHPYTRGLLDSVPSRNVPGRPL
                     AQIRGMTPSLASLDEGCSFRERCARALDECRIAPETRAPSAGRTLRCRNPMP"
     gene            2702..>2920
                     /locus_tag="VF309_02125"
     CDS             2702..>2920
                     /locus_tag="VF309_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465408.1"
                     /translation="MSAATASLLELRAVSKRFVRGLDLAARIARALGAKASEAIVHAV
                     DHVDLAVREGEVVGLVGESGCGKSTLGRM"
BASE COUNT          428 a         1025 c          934 g          533 t
ORIGIN      
        1 cggccgatcg ttcgtctaca acattcccgc gatccggctg atcctcgaaa agcttccagc
       61 cacgctcgag ctcgccgtgt cggcgatggt catcgccgtc gtgatcggca tcccgctggg
      121 gctcgttgcg ggactcaagc ccgacacatg gcttggccgc ggcatcatgg ccggctccat
      181 cgtcggattc agcctgccga ccttctgggt gggcctcgtg ctcatcatgg tcttctcggt
      241 gcagctcgga tggctgccgt cgaacggccg cggcgagacc gtgctgcttc tgggcgttcc
      301 ggtgagtttc ctcacgctcg acgggctcgc gcacctcgcg ctgccggcgc tgaacctggc
      361 gctcttcaag ctctcgctgc tggttcggct cacgcgcgcg ggcacgcgcg aagcgctcct
      421 gcaggactac gtcaaattcg cgcgcgccaa gggtctcggc ccgggacgcg tgatcggcgt
      481 gcacgttctg aagaacatcc tcatccccat cgtcaccgtg atcggcctcg agctgggaag
      541 cgtgatcgcg ttcgccatcg tgaccgaatc gatcttcgcg tggcccggca gcggcaagct
      601 tctcatcgac tcgataaacc agctcgaccg cccggtgatc gtcgcctacc tcatggtgac
      661 ggtgacgatc ttcatcgtca tcaacttcgt ggtcgatgtt ctctactcgc tgctcgaccc
      721 gcgcgttcgc ctcggcggcg cggcgccggc gcactgacgg ccatgggcat cgcggctccc
      781 gaagcgcccc aaaccgcggc gaccagcgcc gggcctccct ctatggccgg cgtcgaatcg
      841 ccctggcgcc gcttcgtttc ggaattcgcc gagagcggcc ttgcgttggc cggtctcgtc
      901 ctgctcgtcg cgctcgtact ggtggcgata gccgctccct acatctctcc gcagaatcca
      961 tacgacctct cgcaactcga cgtcctggac tcgcggctcg cccccgggga acactcgtcc
     1021 acctccggga agccgtactg gctcggaacc gacgaccagg ggcgcgacat gctctccggg
     1081 atcttctacg gcttgcgcat ctcgctcgcc gtcggcgtgg ccagcacggc gatcgcgctc
     1141 gccatcggcc tcgtgctcgg gctcgcggcc gcgtacttcg gcggctgggt cgatggcgtg
     1201 atcatgcgca tagccgacat ccagctgtcc ttccccgcga tcctcatcgc tctcatcctt
     1261 ctcgcgatcc tgggccaggg ggtgggaaag atcatcgcgg cgctggtcac catccagtgg
     1321 gcgtattacg cgcgcacggt acggtcggct gcgctggtcg agcggcagaa ggaatatgtc
     1381 gaggcggcga aagtgctcgc cctttcgcag ccgcggatcg tgtttcgcca cctcctgccg
     1441 aactgcctgc cgcccttgat agtcgtcgcc accgtgcaga tcgcgcacgc catctcgctg
     1501 gaggcgaccc tttccttcct cgggctgggg ctgccgatca ccgagccgtc gctgggcctt
     1561 ctcatcgcga acggctttca gtacctcctc tcgggcaagt actggatcag cttctatccg
     1621 ggcctcgcgc tgctgctgac gatcgtctcg atcaacctcg tcgccgacca gttgcgcgac
     1681 gtgttgaatc ctcgcctcag gaaataggag gcgcgacaat gtcccatgca gccacgcgtt
     1741 cgggcgccgc gcttctcgag gtcgaggggc tgcgcatgca tttcttcacc aaggcgggcg
     1801 tcgtgaaagc ggtcgacgac gtgagctttc gggtcgagcg cggccgcatc ctggggctcg
     1861 tgggcgagtc ggggtccggc aagtcgatga cgggctattc gatcctcggc ctcgtcgacg
     1921 cccccgggcg aatcgtggcg ggaagcatcc ggctcaacgg caccgacatc acgaggctcg
     1981 gcgagccggc ctggcgcaag cttcgcggcg agcgcatcgc gatgatcttc caggacccga
     2041 tgatgacgtt gaatccggtg ctgcgcatcg atacgcagat gatggaagcc gtgcgcgcgc
     2101 accatgacgt ctcccgcggc gcggcgcgcg agacgtgccg cgaggcgctc gcacgcgtcg
     2161 gaatcgcatc gcccgaggag cgcctgcgca actatcccca ccagttctcc ggcgggatgc
     2221 gccagcgggt cgcgatcgcg atcgcgcttc taaaccggcc cgacctcatc atcgccgacg
     2281 agccgacgac ggcgctcgac gtgaccatcc aagggcagat actcttcgag atgcagaaac
     2341 tctgccgcga aacgggggcc ggcctcatct ggatcacgca cgatctttcc gtgatctcgg
     2401 ggctcgccga cgaggtctgc gtgatgtacg ccggccgcat cgtcgaatcc ggcacggtgg
     2461 acgagatcct ggatcgcccg ctgcaccctt acacgcgcgg actgctcgac tccgtgccca
     2521 gccgcaacgt ccccggccgc ccgctcgcgc agatccgcgg catgacgcca tcccttgcca
     2581 gcctggacga ggggtgctca ttccgcgagc gctgcgcgcg cgccctcgac gagtgccgca
     2641 tcgcgcccga gacgcgcgcg ccatcggcgg gtcgtacgct gcgctgccgc aatccgatgc
     2701 catgagcgcc gcgaccgcgt ccctgctgga gcttcgcgcc gtgtcgaagc gtttcgtgcg
     2761 tggcctcgac ctcgcggcgc gaatcgcgcg cgccctgggc gcgaaagcaa gcgaagcgat
     2821 cgtgcatgcg gtcgaccacg tcgacctcgc ggtgcgcgaa ggagaggttg tcggcctcgt
     2881 cggagagtcg gggtgcggga aatcgacgct gggacgcatg
//
LOCUS       DASVII010000164         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_223461,
            whole genome shotgun sequence.
ACCESSION   DASVII010000164 DASVII010000000
VERSION     DASVII010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223461"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..438)
                     /locus_tag="VF309_02130"
     CDS             complement(70..438)
                     /locus_tag="VF309_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465409.1"
                     /translation="MDRQTQVCVEDGVVRVTVRGEVKLAAGGAAIAQASRVAQDAGSN
                     LLLFDITQAQSREYHAVAMHHARDANRTGIVKFRIAILGKAGDAKLSFLENVARNRGI
                     RARSFTAEREAVRWLKASSP"
     gene            complement(497..1249)
                     /locus_tag="VF309_02135"
     CDS             complement(497..1249)
                     /locus_tag="VF309_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465410.1"
                     /translation="MKARIQAPLATIEVVGTADPTLSWDEVWGLIQRFFDAEREDVEE
                     LIKHHGFVALFRAADDRRLVGLTALDVYPTEFEGRKVAVIFTSHSMLDEAFRGQNLMQ
                     RLGWRTFLRTRLRFPFHAIYWFYDSFSSNGYLLLPRNFRDFWPRRDAPTPAWEQGLMN
                     HLAVQMYREAWQPSLGIVRRTGHKRLRAETARPQAEYDRDPDLAFYARANPGHAEGDM
                     LVCLCPLTLVNLVTLGVRARQRRLRQEAAGDR"
     gene            complement(1246..1659)
                     /locus_tag="VF309_02140"
     CDS             complement(1246..1659)
                     /locus_tag="VF309_02140"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX7465411.1"
                     /translation="MPAARCTYDYAVIRVVPRVEREEFVNAGVIVSCPEMRFLEARIE
                     LDEARLVALDPAIDLDAVRAHLAAIPAICAGGADAGAIGLMPQKERFRWLVAPRSTVV
                     QTSPAHAGRCENPGALLEHLLATMVRPPKPARELA"
     gene            complement(1634..>2222)
                     /locus_tag="VF309_02145"
     CDS             complement(1634..>2222)
                     /locus_tag="VF309_02145"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX7465412.1"
                     /translation="LPVPEIVLIELDVELARAEPDPEIQELIKASAGLNLALDYLPGS
                     VTFDPVADSVDATLASKIVWFDAFVTNIDRSPRNTNMLLWHRKLWLIDHGAALYAHHG
                     SGDLAGRAREPFARVKDHVLLPIASELGEVDAGLAARFTPGLLREIVELVPAEWLGDA
                     PEARRAAYTGYLAARLERPRPFLEEAIRARSALHV"
BASE COUNT          358 a          754 c          777 g          333 t
ORIGIN      
        1 cggcgcatcc tgcggattca tgggcgaatt atacagcgcg cccgcgccca cttcagcccg
       61 tgggcgcgat catggggacg acgccttgag ccacctcacg gcttcgcgct cggcggtgaa
      121 gctgcgggcg cgaatgcccc gattgcgcgc gacgttctcg agaaacgaga gcttggcgtc
      181 gcccgccttg ccgaggatcg cgatgcggaa cttgacgatg cccgtgcgat tggcatccct
      241 cgcgtggtgc atcgcgacgg cgtgatattc gcgcgactgg gcctgggtga tgtcgaagag
      301 gagcaggttg ctgcccgcgt cctgcgcgac acgcgaggcc tgggcgatcg ccgcgccgcc
      361 ggccgcgagc ttcacctcgc cccgtaccgt gacccgcacg accccgtctt ccacgcacac
      421 ctgcgtttgg cggtccatga acccatggtg cggcagtcga tccccgagaa tccccacgct
      481 tccatgtggg agccgatcaa cggtcgcccg ccgcctcctg gcgcagacgg cgttggcgag
      541 cgcgcactcc gagcgtcacc aggttcacca gcgtgagcgg gcagaggcaa accagcatgt
      601 cgccttcggc atgacccggg ttggcccgcg catagaaggc gagatccgga tcgcggtcgt
      661 actcggcctg cgggcgcgcc gtttcggcac gcaggcgctt gtgtcccgtg cggcgcacga
      721 tgccgagcga gggttgccac gcttcgcgat acatctggac cgcgagatgg ttcatgagtc
      781 cctgctccca ggccggcgtg ggagcgtcgc gccgcggcca gaagtcgcgg aagttgcgcg
      841 gcagcagaag gtagccgttg gagctgaacg agtcgtagaa ccagtagatc gcgtgaaacg
      901 ggaagcgcag gcgcgtgcgc aggaacgtgc gccagccgag gcgctgcatc aggttctggc
      961 cgcgaaaggc ctcgtcgagc atcgagtggg aagtgaagat cacggcgacc ttgcgcccct
     1021 cgaactcggt cggatacacg tcgagagcgg tgaggccgac gagacgcctg tcgtccgccg
     1081 cgcggaagag cgcgacgaag ccgtggtgct tgatgagctc ctccacatcc tcgcgttccg
     1141 cgtcgaagaa gcgctggatg aggccccata cctcgtccca ggaaagcgtc gggtcggccg
     1201 tccccaccac ttcgatcgtg gcgagaggcg cttggatcct cgccttcaag cgagctcccg
     1261 agccggcttc ggcggccgca ccatcgtcgc gagcaggtgc tcgaggagcg cgcccggatt
     1321 ctcgcagcgc cccgcgtgcg cgggcgacgt ctgcaccacg gtgctcctgg gcgccacgag
     1381 ccagcgaaag cgttccttct gcggcatgag gccgatcgcg cccgcgtccg cgccgcccgc
     1441 gcaaatcgcc ggaatcgccg ccaggtgcgc gcgcaccgcg tccaggtcga tcgccggatc
     1501 gagcgccacg aggcgcgcct cgtcgagctc gatgcgcgcc tcgaggaagc gcatttcggg
     1561 gcacgacacg atgacgccgg cattcacgaa ctcctcgcgc tcgacgcgcg ggacgacgcg
     1621 aatcaccgcg tagtcatacg tgcagcgcgc tgcgggcacg gatcgcctcc tcgaggaacg
     1681 ggcgcgggcg ctcgagccgc gccgcgagat agccagtgta agccgcgcgg cgcgcttcgg
     1741 gcgcgtcgcc gagccattcc gcgggcacga gctccacgat ctcgcgcagc agccccgggg
     1801 tgaagcgcgc ggcgaggccg gcgtcgactt ccccgagctc gctcgcgatc gggagcagca
     1861 cgtgatcctt cacgcgcgcg aacggctcgc gcgcgcgccc ggcgagatcg ccgctgccgt
     1921 gatgcgcata gagcgcggcg ccgtggtcga tgagccacag cttgcgatgc cacagcagca
     1981 tgttggtgtt gcgcggcgag cggtcgatgt tggtgacgaa cgcgtcgaac cagacgatct
     2041 tcgaggcgag cgtcgcatcg acgctatcgg cgaccgggtc gaaggtgacg gatcccggca
     2101 ggtagtcgag cgcgaggttc aatccggcgc tcgccttgat gagctcctgg atctccggat
     2161 cgggctcggc gcgcgccagt tcgacatcca actcgatcag gacgatctcg ggcaccggaa
     2221 gg
//
LOCUS       DASVII010000165         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_475241,
            whole genome shotgun sequence.
ACCESSION   DASVII010000165 DASVII010000000
VERSION     DASVII010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475241"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VF309_02150"
     CDS             <1..938
                     /locus_tag="VF309_02150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7465413.1"
                     /translation="ARRARRILPAYYAQLVLLFAVVLPLLRGLDYWRSDLYVYVYNLV
                     THATFLHNTTPLSSGSMQVNGALWTLAVEVQFYLLVPLVAPLVVRWPGRALAVSFALA
                     IAWRLATDDGLGPLVAFEIALGRPWGWTEGVVRYLLLHQLPSYCGHFVLGMVLGGAWL
                     SWREGEHSRVARRALDAGLVLALALLYWAIAIDGRVAGSLAWAIPPLCLGFLLWRAAL
                     GGRAARILASGPLAFAGRVSYSAYLLHLPMLFLWNRGAATLAPWLSLPLYLAAVFSLS
                     WLSWRFIERPFLRRGSARSGPRVPMNDEMPLANRP"
     gene            complement(950..>2173)
                     /locus_tag="VF309_02155"
     CDS             complement(950..>2173)
                     /locus_tag="VF309_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7465414.1"
                     /translation="MSAANPAAASVRQDATVISVVGFAHGTSHFFHFVIPPLFLWLMR
                     DFSLNFTQVGAIMTTFYVVSGIGQALAGFAVDRFGGMRVLCGGVALLSLGGLAYASAQ
                     SYGMLLGAAMVAGLGNSVFHPADYTIMNRRVSTQRLGHAFSVHGLSGTLGWAAAPVFV
                     LAIAGTMGWRAACVGASIVGFLALGVLLSCRGVLDDSSRHLPQIAATREGGSFAFLGV
                     GVVWMCFAFFLISVMAFGALQNFAPPLLERAYGVSLAFATSALTAYLLGSAAGTATGG
                     FFATGGEREDRRVAISLGAAALCAVALASGSVPSWSVVALMGCMGFSVGFIGPSRDML
                     VRRAATSTFGTGAFGRVYGFVYSGIDAGLALAPLAFGPLMDSGHYTQVLWGVALLQVL
                     AIGTALGVGSRARSQ"
BASE COUNT          348 a          767 c          743 g          315 t
ORIGIN      
        1 acgcgcgccg cgcgcgccgc atcctgcccg cctactacgc gcagctcgtg cttctcttcg
       61 cggtggtgct gcccctcctg cgcggcctgg actactggcg atcggacctg tacgtgtacg
      121 tgtacaacct cgtcacccac gcgaccttcc tgcacaacac gacgccgctc tcgagcggct
      181 cgatgcaggt gaacggcgcg ctgtggacgc ttgcggtcga agtgcagttc tacctgctcg
      241 tgcccctggt cgcgccgctc gtggtgcgtt ggcccggccg ggcccttgcc gtgtccttcg
      301 ctctggcgat cgcatggcgg ctcgccacgg acgacggact cgggccgctc gtcgcgttcg
      361 agatcgcgct gggccgtccc tggggatgga ccgagggcgt ggttcgatac ctgctcctgc
      421 accagctgcc gagctattgc ggccacttcg tcctcgggat ggtcctcggg ggcgcatggc
      481 tatcgtggcg cgagggcgag cattcgcgcg tggcgcggcg cgcgctcgac gcggggctcg
      541 tgctcgctct cgccctcctc tattgggcga tcgcgatcga cggccgcgtc gcaggcagcc
      601 tcgcctgggc gattcccccc ttgtgccttg gattcctgct ctggcgagcc gcgctgggcg
      661 gccgcgcggc gaggatcctc gccagcggac cgctcgcgtt cgcggggcgc gtgagctact
      721 ccgcctatct gttgcacctg ccgatgttgt tcctctggaa ccgcggcgcg gcgacgcttg
      781 caccgtggtt gtcgttgccg ctctatctcg cggcggtgtt ctccctgtcg tggttatcgt
      841 ggcgtttcat cgagcgcccg ttcctgcgcc gcgggagcgc ccgcagcgga ccccgcgtgc
      901 ccatgaacga cgagatgccg ctggccaacc ggccctaggg cgcgcctcgc tactgcgagc
      961 gagcacgcga gcccacgccg agcgcggtgc cgatggcgag gacctgcagc aacgcgacgc
     1021 cccagaggac ctgggtgtag tgtcccgaat ccatgagcgg accgaaggcg agcggcgcga
     1081 gcgcgagtcc cgcgtcgatg cccgagtaca cgaagccgta gacgcggccg aaggcgccgg
     1141 tgccgaaggt gctcgtggcg gcgcgccgca ccagcatgtc gcgcgaaggc ccgatgaatc
     1201 ccacgctgaa tcccatgcat cccatgagcg cgaccacgct ccagctcggg accgacccgc
     1261 tggcgagcgc caccgcgcag agcgccgcgg ctcccagcga tatcgcgacg cgccggtctt
     1321 cgcgctcgcc acccgtcgcg aagaatccgc cggtcgcggt tcccgccgcg cttcccagga
     1381 ggtaggccgt cagggccgac gtcgcaaacg cgagcgacac gccataggcg cgctcgagaa
     1441 gcggcggcgc gaaattctgc agcgccccga acgccatcac cgagatcagg aagaaggcga
     1501 agcacatcca cacgacgccc acgcccaaga acgcgaacga gcccccctcg cgcgtcgccg
     1561 cgatctgcgg caggtgacgc gacgaatcgt cgaggacgcc ccggcaggaa aggagaacgc
     1621 ccaacgcgag gaatcccacg atcgaagccc cgacgcacgc ggcgcgccac cccatcgtcc
     1681 cggcgatggc gagaacgaag accggcgccg cggcccagcc cagcgtgccc gagagcccgt
     1741 gcaccgagaa tgcgtggccg aggcgctggg tcgacacgcg gcggttcatg atggtgtagt
     1801 cggcgggatg gaagacgctg ttgccgagcc ccgcgaccat cgcggcgcct aggagcattc
     1861 cgtagctttg cgccgaggcg taggcgaggc ctcccaggga aagcagcgca acgcccccgc
     1921 aaaggacgcg catgccgccg aaacgatcga ccgcgaatcc cgcgagcgcc tgccctatcc
     1981 cggaaacgac gtagaacgtc gtcatgatcg cgccgacctg ggtgaaattg agcgagaaat
     2041 cgcgcatcag ccagaggaag aggggaggaa tgacgaaatg gaagaaatgc gacgtgccgt
     2101 gggcgaatcc caccacggaa atgacggtcg cgtcctggcg gacgctcgcg gcggcgggat
     2161 tggcggcgga cat
//
LOCUS       DASVII010000166         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_7996, whole
            genome shotgun sequence.
ACCESSION   DASVII010000166 DASVII010000000
VERSION     DASVII010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7996"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VF309_02160"
     CDS             <1..682
                     /locus_tag="VF309_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7465415.1"
                     /translation="EVNPARRYQSAAHLAFDLRNPGQVELTARATKSYREGVIAQAAR
                     WWKARDIGVTPGPARDEPPVAPIVMVAVDTLHPDDSRQPELQRATRRILSLSREFRLI
                     CVSVVPSGLDAKAAKGAGDAQVEHRIRLRQWIHPLRIETSRVSLHVLESGDAAGALVD
                     FARRNNVDLIVLGAPAPGERTFAWWRSVASSVTANAPCSVHVVRRTGEGADPPGLDLD
                     QLPPPATG"
     gene            692..>1765
                     /locus_tag="VF309_02165"
     CDS             692..>1765
                     /locus_tag="VF309_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7465416.1"
                     /translation="MDTIDVDVVIIGGGGAGLRAAIAAAESNPALTIALVSKVYPMRS
                     HTVAAEGGAAAVTLAEDSLEHHFNDTVSGGDWLCDQDAVEYFVEHCREEMVQLEHWGC
                     PWSRREDGRVNVRAFGGMKIERTWFAADLTGFHMLHTLFQTSMKYPSIRRFDEHFCCD
                     LLVDEGRVQGVVAIDIATGEFTMILARAVIIATGGAGRVFRENTNGGIVTGDGMALAY
                     RRGVPLRDMEFVQYHPTCMPGTGLLFTEACRGEGGILVNKDGYRYLQDYGLGPPDPWP
                     RKKAMELGPRDRLSQAFWHEQRKGRTIETPHGPAVHLDLRKLGERKLRERLPQICELA
                     RDFLGIDPAQTPIPVRPSVHYTMG"
BASE COUNT          275 a          625 c          608 g          257 t
ORIGIN      
        1 cgaggtcaat cccgcacggc gctaccagtc ggcggcgcac ctggccttcg acctgcgcaa
       61 tcccggccag gtcgagctta cggcgcgcgc gacgaagtcg taccgcgagg gagtgatcgc
      121 ccaggccgcg cgctggtgga aggcgcgcga catcggcgtg actcccggtc cggcgaggga
      181 cgagcccccg gtggcaccca tcgtgatggt ggcggtcgac acgctccacc ccgacgactc
      241 ccgccagccg gagctgcagc gcgcgacgcg gcgcatcctc tcgctcagcc gcgagttccg
      301 gttgatctgc gtgtcggtgg tgccctcggg gctcgacgcg aaggcggcga agggcgcagg
      361 cgatgcgcag gtcgagcatc gcatccgcct gcgccagtgg atccatccgc tgcggatcga
      421 gacgagccgc gtctcgctcc acgtcctcga gagcggggac gcggcgggcg ccctggtgga
      481 cttcgcccgg cgcaacaacg tcgacctcat cgtgctgggg gccccggcgc ccggggaaag
      541 gaccttcgcc tggtggcgct cggtggcctc gtcggtcacc gccaacgcgc cgtgcagcgt
      601 gcatgtcgtg cgccgcacgg gcgaaggcgc agacccgcct ggacttgacc tcgatcaatt
      661 gccacctccc gccacgggct agtttccgta gatggatacg atcgacgtgg acgtcgtcat
      721 catcggaggg ggcggcgcgg ggctgcgcgc ggcgatcgcc gcggccgaat ccaatcccgc
      781 gctgaccatc gcgctcgtct cgaaggtcta cccgatgcgc agccacacgg tcgccgcgga
      841 aggcggggcc gcggccgtca cgctcgccga agacagcctc gagcaccact tcaacgacac
      901 cgtctccggc ggcgactggc tctgcgacca ggacgcggtg gagtatttcg tcgagcattg
      961 ccgcgaggag atggtccagc tcgagcactg gggctgcccc tggagccggc gcgaggacgg
     1021 ccgcgtcaac gtgcgcgcct tcggcggcat gaagatcgag cgcacctggt tcgccgcgga
     1081 cctcaccggc ttccacatgc tgcacacgct cttccagacg tccatgaaat atccctccat
     1141 ccggcgcttc gacgagcact tctgctgcga tctgctggtg gacgaggggc gcgtccaggg
     1201 cgtggtcgcc atcgacatcg ccacgggcga gttcaccatg atcctcgcgc gcgccgtgat
     1261 catcgccacg ggcggcgcgg gacgcgtgtt ccgggagaac accaacggcg gcatcgtgac
     1321 cggggacggc atggcgctcg cctatcgccg cggcgtgccg ctgcgcgaca tggagttcgt
     1381 gcaataccat ccgacctgca tgcccggcac gggactgctc ttcaccgagg cgtgccgcgg
     1441 cgagggcggc atcctcgtga acaaggacgg ctaccgttac ctgcaggact acggcctcgg
     1501 cccgccggat ccgtggccgc gcaagaaggc gatggagctg ggcccgcgag atcgactctc
     1561 gcaggcgttc tggcacgagc agcgcaaggg ccgcaccatc gaaacgcccc atgggcccgc
     1621 ggtgcatctc gacctgcgca agctcggaga acgaaagctg cgcgagcgcc tgccgcagat
     1681 ctgcgagctc gctcgcgact tcctcggcat cgatcccgca cagacgccga tcccggtgcg
     1741 cccttcggtg cactacacga tgggg
//
LOCUS       DASVII010000167         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_115913,
            whole genome shotgun sequence.
ACCESSION   DASVII010000167 DASVII010000000
VERSION     DASVII010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115913"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>2104
                     /locus_tag="VF309_02170"
     CDS             17..>2104
                     /locus_tag="VF309_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465417.1"
                     /translation="MSKVPSPAPKIATPTAASSPREVPALESLVPGFADNKLAFVDVK
                     DGDTLAYAQSNVRVKGVAGSTFKLTVNGAELPDSRVGKRSVLAEKEVQAWEFIGVELK
                     PGANTLTVVQVDSFGNARGSETIRVLAPGKLARLVIELPAAGGIADGKTPVKVVVTLA
                     DANGVPVTVRTAVTLEASKGRWQVEDLDANEPGVQVFVEGGRGEFLLTPPLEAGESQV
                     YASSGRLKAQARLDFLPELRNMIAAGVIEGIVNVRNINTRALVPARASDAFEQEIRQL
                     SRDWNGGKSQAGARAAFYLKGKIKGEYLLTAAYDSDKDTQERLFRDIQPDEFYPIYGD
                     SAVRGFDAQSTSKLYVRVDKGRSYLLFGDFTTNSPTEARKLSNYNRSLTGIKEHYENS
                     RVSVNAFAARDTTRQVIEELRANGTSGPFQLGTQGALVNSETVEIIARDRNQPAIIVS
                     SVPQTRFSDYEIEALTGRILFKAPVPSVDQNLNPVFVKVTYEVDQGGPQFWVAGVDAQ
                     VKLGDRVEVGGVYVKDKNPLAPFTLGGANMTVKLGAATYIITEVARSETGLDDVKGNA
                     ERIEFKHDSKDLKATAYVARTDLGFENPGAYLTQGRSEAGGKLDYKISDRLTLRAEAL
                     RTADLATSSTRDGEAVSITYQIAEKLSFELGVRHASEKDGATAASPIPVVDGQPAPTP
                     NPGDVTSVRARLTG"
BASE COUNT          376 a          738 c          701 g          289 t
ORIGIN      
        1 ggtggctccc gcggtggtgt cgaaggttcc gagcccggcg ccgaagatcg cgacgcccac
       61 cgccgcgagc tcgccgcgcg aggtgccagc gctggagagc ctcgtccccg gcttcgccga
      121 caacaagctc gccttcgtcg acgtgaagga tggcgacacg ctcgcgtacg cgcagtccaa
      181 cgtgcgcgtg aagggtgtcg ccggcagcac cttcaagctc accgtcaacg gcgccgagct
      241 tcccgattcg cgcgtcggca agcgctcggt gcttgccgag aaggaagtgc aggcgtggga
      301 attcatcggc gtcgagctga agcccggcgc caacacgctg acggtcgtgc aggtggattc
      361 gttcggcaac gcgcgcggct ccgagacgat ccgcgtgctc gcccccggca agctcgcgcg
      421 gctcgtgatc gagcttcccg ccgcgggcgg catcgccgac ggcaagactc cggtgaaagt
      481 cgtcgtgacg ctcgccgacg cgaatggcgt gccggtcacc gtgcgcacgg cggtcacgct
      541 cgaagcgtcc aaaggccgct ggcaggtcga ggacctcgac gccaacgagc ccggcgtgca
      601 ggtgttcgtc gagggcgggc gcggcgagtt cctccttacc ccgccgctcg aggcgggcga
      661 gagccaggtc tacgcttcga gcggtcgcct caaggcgcag gcgcgcctcg acttcctgcc
      721 ggagctgcgc aacatgatcg ccgccggcgt gatcgagggc atcgtcaacg tgcgcaacat
      781 caacacgcgc gcgctcgtgc cggcgcgcgc ctccgacgcc ttcgagcagg agatccgcca
      841 gctgtcgcgc gactggaacg gcggcaagag ccaggcgggc gcgcgcgcgg ccttctacct
      901 caagggcaag atcaagggcg aatacctcct caccgcggcc tacgattccg acaaggacac
      961 gcaggagcgc ctcttccgcg acatccagcc cgacgagttc tacccgatct acggcgactc
     1021 ggcggtgcgc ggattcgacg cgcagtcgac gagcaagctc tacgtgcgcg tggacaaggg
     1081 ccgctcgtac ctcctgttcg gcgacttcac gaccaactct cccacggagg cccgcaagct
     1141 cagcaactac aaccgctcgc tcaccgggat caaggagcac tacgagaatt cccgcgtgag
     1201 cgtgaacgcg ttcgccgcgc gcgacacgac gcgccaggtg atcgaggagc tgcgcgccaa
     1261 cggcacctcg ggcccgttcc agctcggcac ccagggcgcg ctcgtgaaca gcgagacggt
     1321 cgagatcatc gcgcgcgacc gcaaccagcc ggcgatcatc gtctcgagcg ttccgcagac
     1381 gcgtttctcc gactacgaga tcgaggcgct cacgggacgc atcctcttca aggcgccggt
     1441 gccttcggtc gaccagaacc tgaacccggt cttcgtgaaa gtcacctacg aagtcgacca
     1501 gggcggcccg cagttctggg tcgcgggagt cgacgcgcag gtgaagctcg gcgaccgcgt
     1561 cgaggtgggc ggcgtctacg tgaaggacaa gaacccgctc gcgccgttca cgctcggcgg
     1621 cgccaacatg acggtgaagc tgggcgcggc gacctacatc atcaccgagg tggcgcgcag
     1681 cgaaaccggc ctcgacgacg tgaagggcaa cgccgaacgc atcgagttca agcacgactc
     1741 gaaggacctg aaggcgacgg cgtacgtcgc gcgcaccgac ctcggcttcg agaatccggg
     1801 cgcgtacctg actcaaggac gcagcgaagc gggcggcaag ctcgactaca agatctccga
     1861 tcgcctcacg ttgcgcgccg aggccctgcg caccgcggac ctggcgacga gctcgacgcg
     1921 cgacggcgaa gcggtgtcga tcacatacca gatcgccgaa aagctttcct tcgagctggg
     1981 cgtgcgccac gcctcggaaa aggatggcgc tacggccgcc tcgccgatcc cggtggtcga
     2041 cggccagccg gccccgactc cgaaccccgg cgatgtcaca tcggtgcgcg cgcgcctcac
     2101 cggc
//
LOCUS       DASVII010000168         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_691138,
            whole genome shotgun sequence.
ACCESSION   DASVII010000168 DASVII010000000
VERSION     DASVII010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691138"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VF309_02175"
     CDS             complement(<1..229)
                     /locus_tag="VF309_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7465418.1"
                     /translation="MSPTLVRKKPIYLDYSATTPVDPRVAEKMIPWLTEQFGNPASRS
                     HGYGWEAEEAVENARVEVAKLVNADAKEIVWT"
     gene            complement(234..701)
                     /gene="iscR"
                     /locus_tag="VF309_02180"
     CDS             complement(234..701)
                     /gene="iscR"
                     /locus_tag="VF309_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEX7465419.1"
                     /translation="MRLTTKGRFAVTAMLDLALHGASGPVTLAGISERQKISLSYLEQ
                     LFGKLRRRELVESVRGPGGGYHLARDAGEVTVADIICAVEEPIDSTQCGGRENCHDSH
                     RCMTHDLWEDLNTAVYGFLAGVTLRQLVEHQRTKPVSLVRSHSKIREPHLLSS"
     gene            complement(813..1526)
                     /gene="cysE"
                     /locus_tag="VF309_02185"
     CDS             complement(813..1526)
                     /gene="cysE"
                     /locus_tag="VF309_02185"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX7465420.1"
                     /translation="MFSRIREDIQCVFERDPAARSVWEVITCYPGFHALQMHRFSHAL
                     WRMGLRWLARFTSHIARFLTGVEIHPGAAIGRRVFIDHGMGVVVGETAEIGDGCTLYH
                     GVTLGGVSWNKGKRHPTLGKNVVVGAGAKILGPFIVGEGAKIGSNSVVVKAVPAGATV
                     VGIPGRIVEAGPGHADAGRMAFDAYAVSADIDDPLNKVLLALGTRTEDFDGRLAEILA
                     RLEGLESSSNAADLRRKTG"
     gene            1637..>2150
                     /locus_tag="VF309_02190"
     CDS             1637..>2150
                     /locus_tag="VF309_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7465421.1"
                     /translation="MKRIFQRYVEFIRQPDVARLLLVALLARMPVGMVGFAMLMFLRD
                     ALGNFALAGSAVGIHFISMAAAAPVIGRIIDRHGPRTLLLVTGIVQPIALLGILACAN
                     LRLPYAAIAGCAALAGAFATPITTLTRTSWRHRFESEEDRRTAFALDSVMIEINFTLG
                     PAIVAGILATL"
BASE COUNT          351 a          742 c          677 g          380 t
ORIGIN      
        1 aggtccagac gatctccttc gcgtcggcgt tcacgagctt ggcgacctcg acgcgcgcgt
       61 tctcgacggc ctcctccgct tcccagccgt agccgtggct gcgcgatgcc ggattgccga
      121 actgctccgt cagccacggg atcatcttct ccgccacgcg cggatcgacc ggcgtcgtcg
      181 cggaatagtc gaggtagatc ggcttcttgc gcacgagggt ggggctcatg gggtcaggac
      241 gataagaggt gcggttcgcg aatcttcgaa tggctgcgga cgagggaaac gggcttggtg
      301 cgctggtgct cgacgagctg gcgcaacgtc acgcccgcga ggaacccgta caccgcggtg
      361 ttcaaatcct cccacaggtc gtgcgtcatg cagcgatggc tgtcgtggca gttctcgcgg
      421 ccgccgcatt gcgtcgaatc gatgggctcc tcgaccgcgc agatgatgtc cgcgacagtg
      481 acctcgcccg cgtcgcgcgc gaggtggtag ccgccgcccg ggccgcgcac gctttcgacc
      541 agctcgcgcc ggcgcagctt gccgaagagc tgctcgagat aggagaggga gatcttctgg
      601 cgctcgctga ttccggccag cgtgaccgga cccgaggcgc catggagcgc gagatcgagc
      661 atcgcggtca ccgcgaagcg tcctttggtc gtcagtctca tgggttcctc ggctgggaac
      721 gcccgggaat ttcaatcccg agcgattcgc tcaagtatac aaagcccgac tgaattagtc
      781 aactaatggc ccctggcaag cgcctaagcc aatcaaccgg tttttcgccg aagatcggcg
      841 gcgttcgacg aactttcgag cccttccaga cgggccagga tctccgcgag gcgcccgtcg
      901 aagtcctcgg tgcgggtccc gagcgccagc aagaccttgt tcagcgggtc gtcgatatcg
      961 gccgatacgg catacgcgtc gaaagccatg cgaccggcat cggcatggcc gggtcccgcc
     1021 tcgacgatgc gccccggaat cccgaccacc gtcgcgccgg ccgggaccgc cttgaccacg
     1081 accgagttgg agccgatctt cgcgccctcg cccacgatga acgggcccag gatcttcgcc
     1141 ccggccccga cgaccacgtt ctttccgagc gtcggatggc gcttgccctt gttccaggaa
     1201 acgcctccca gggtcacgcc gtgatagagc gtgcagccgt cgccgatctc cgcggtctcg
     1261 ccgacgacca cccccatgcc gtggtcgatg aagacgcgcc ggccgatggc cgcgcccgga
     1321 tggatctcga cgcccgtgag gaagcgcgcg atgtgcgagg tgaagcgcgc gagccagcgc
     1381 agccccatcc tccacagcgc gtgcgagaag cgatgcatct gcagcgcgtg gaagccggga
     1441 tagcacgtga tcacctccca cacggagcgg gcggcgggat cgcgttcgaa aacgcactgg
     1501 atgtcttcgc gaatgcggct gaacatttgg agtccgcgga gtctgcggtc gcgttgtatg
     1561 gcgtacaacg cagtttatta ttcccgagta aaattatcaa ctattcaccc ggtggggtga
     1621 gcccgaatcc cttccggtga aacgcatttt ccaacgctac gtcgagttca tccgacagcc
     1681 cgacgtcgcc cgactcctgc tcgtggcgct cctcgcgcgc atgcccgtcg gcatggtggg
     1741 cttcgcgatg ctcatgttcc tgcgcgacgc gctcggcaac ttcgcgctcg ccgggagcgc
     1801 ggtcggaatc cacttcatct cgatggccgc cgccgcgccc gtcatcggcc gcatcatcga
     1861 tcgccacggg ccgcgcaccc ttcttctcgt gacgggaatc gtccagccca tcgcgctcct
     1921 cggaatcctc gcgtgcgcca atctgcgcct gccgtacgcg gcgatcgcgg gctgcgcggc
     1981 gctcgccggc gcattcgcca cgcccatcac caccctcacc cgcacctcct ggcgccaccg
     2041 gttcgaaagc gaggaggatc gccgcaccgc gttcgcgctc gactcggtga tgatcgagat
     2101 caatttcacc ctcggtcccg cgatcgtggc gggcatcctc gcgacgctgg
//
LOCUS       DASVII010000169         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_44043, whole
            genome shotgun sequence.
ACCESSION   DASVII010000169 DASVII010000000
VERSION     DASVII010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44043"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF309_02195"
     CDS             <1..343
                     /locus_tag="VF309_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7465422.1"
                     /translation="HVNKTDSAVRITHLPTNIVVSSQNDRSQHRNRAEAMAMLKSKLY
                     ELELRKKTEAKQKMEDAKTDIGWGHQIRSYVLDQSRIKDLRTNVEVGNTQSVLDGDLD
                     QFISASLKQGV"
     gene            347..>1611
                     /gene="lysS"
                     /locus_tag="VF309_02200"
     CDS             347..>1611
                     /gene="lysS"
                     /locus_tag="VF309_02200"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7465423.1"
                     /translation="MANDKNETPPGEPPQDENQIIAERRGKLARLREGGPAFPNDFRR
                     KHLAAELQLAHGAKTKEQLEGEKPQAGVAGRMMLKRVMGKASFATLQDMSGQIQIYIN
                     NDVTGAEIHEAFKHWDLGDILGVEGTVFRTNKGELSVHAAKLRLLAKAVRPLPDKFHG
                     LADMEQRYRQRYVDLIVNPESREVFIKRSKIVQAIREFFVARGYLEVETPMMHPIPGG
                     ASARPFKTHHNALDMELYLRVAPELYLKRLTVGGLEKVFEINRNFRNEGISTRHNPEF
                     TMLEFYEAYQDYHYLMDLTEALLKEVAQKVLGTTTLAYQGAAMDLGRKFDRLTMAEAI
                     AKHNPRYPLAELSKPEYLKVALAPFDIEVFPSDGVGLLQLKLFEATTEEKLVDPTFIV
                     AHPVDVSPLARANDANPAVTDRFELFINGR"
BASE COUNT          337 a          549 c          499 g          226 t
ORIGIN      
        1 gcacgtgaac aagaccgact cggcggtgcg catcacgcac cttcccacca acatcgtggt
       61 ctcctcgcag aacgaccgct cgcagcacag gaaccgggcc gaggcgatgg cgatgctcaa
      121 gtcgaagctc tacgagctgg agcttcgcaa gaagaccgaa gccaagcaga agatggaaga
      181 cgccaagacc gacatcggct ggggccacca gatccgctcc tacgtgctcg accagtcgcg
      241 catcaaggac cttcgcacca acgtggaagt gggcaacacg cagtcggtcc tcgacggcga
      301 cctcgaccaa ttcatcagcg caagcctcaa gcaaggggtc tagaacatgg ccaacgacaa
      361 gaacgagact ccaccgggcg agccgcccca ggacgagaac cagatcatcg ccgagcggcg
      421 cggcaagctc gcgcggctgc gcgagggcgg gcccgcgttt ccgaacgact tccgccgcaa
      481 gcacctcgcc gccgagctgc agctcgccca tggcgcgaag acgaaggagc agctcgaggg
      541 cgagaagccc caggccggag tcgcgggccg catgatgctc aagcgcgtga tggggaaggc
      601 ctcgttcgcg acgctgcagg acatgagcgg ccagatccag atctacatca acaacgacgt
      661 gacgggcgcc gagatccacg aggcgttcaa gcattgggac ctgggcgaca tcctcggcgt
      721 cgagggcacg gtgttccgca ccaacaaggg cgagctctcg gtccacgcgg cgaagctgcg
      781 gctcctcgcc aaggcggtgc ggccgctgcc ggacaagttc cacggcctcg cggacatgga
      841 gcagcgctac cgccagcgct acgtggacct catcgtgaac ccggagtccc gcgaggtgtt
      901 catcaagcgc tcgaagatcg tgcaggcgat ccgcgagttc ttcgtggcgc gcggctatct
      961 cgaggtcgag acgccgatga tgcatccgat cccggggggt gcctcggcgc ggcccttcaa
     1021 gacgcaccac aacgcgctcg acatggagct ctacctgcgc gtcgcgccgg agctctatct
     1081 caagcgcctc accgtgggag ggctcgagaa ggtcttcgag atcaaccgca acttcaggaa
     1141 cgagggcatc tcgacgcgcc acaatcccga gttcacgatg ctcgagttct acgaggcgta
     1201 ccaggactac cactacctga tggacctcac cgaggcgctg ctgaaggagg tcgcgcagaa
     1261 agtgctcggc acgaccaccc tcgcctacca gggcgcggcc atggacctgg gcaggaagtt
     1321 cgaccggctc acgatggccg aggcgatcgc caagcacaac ccccgctatc cgctcgccga
     1381 gctctcgaag cccgagtacc tcaaggtagc gctcgcgcca ttcgatatcg aggtctttcc
     1441 gtccgacggc gtggggctgc tgcagctgaa gctcttcgaa gcgaccacgg aagaaaagct
     1501 ggtcgatccc accttcatcg tcgcccaccc ggtcgacgtc tcgccgctcg cgcgcgccaa
     1561 cgacgccaac cccgccgtga ccgaccgctt cgagcttttc atcaacgggc g
//
LOCUS       DASVII010000170         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_511185,
            whole genome shotgun sequence.
ACCESSION   DASVII010000170 DASVII010000000
VERSION     DASVII010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511185"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..249
                     /gene="ffs"
                     /locus_tag="VF309_02205"
     ncRNA           151..249
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF309_02205"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            268..1887
                     /gene="dnaX"
                     /locus_tag="VF309_02210"
     CDS             268..1887
                     /gene="dnaX"
                     /locus_tag="VF309_02210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7465424.1"
                     /translation="MTYQVLARKWRPRVFEEIVGQSHVVTALTNALDSKRLHHAYLFT
                     GTRGVGKTTLARILAKALNCETNGISSHPCGKCRACVDIDAGRFPDLLEVDAATNTKV
                     DEMRELLDTAQYMPVAGRFKVYIIDEVHMLSRHAFNSMLKTLEEPPEHVKFILATTDP
                     QRLPVTILSRCLQFNLKPLTPALIAGHLKHVLTAEGIAFEEPALAQLAKAASGSLRDS
                     LSLLDQAIAHGGGKVAAAQVAEMLGSLGGDLVWQLLERIAEGDGKGTIAEAERIASRS
                     LSLDSALGDLAAILHRIALAQAGAIPADDDPDTQRIGDMSQRIDAGRVQVMYQIALLG
                     RRDLALAPDELAGFTMALLRMLSFAGPANQERPRSEVKVASPRAAPAAAPRANAPSPP
                     APSVASTGPVAFDGDWPALVERLRLTGMAGMAARHAELDSFQNNHLELVVPETDRVYA
                     EKAYQDKLKADLAAHFGPGFRLSVRVGATAGASVAAAKSRETALKQASAAEAIEEDPF
                     VRDLVRDLGAEVVPSSIRPADGAGKQSSDKR"
     gene            1895..2218
                     /locus_tag="VF309_02215"
     CDS             1895..2218
                     /locus_tag="VF309_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7465425.1"
                     /translation="MKGNLGALMKQAQQMQENLKKAQEELAKIEVEGQSGAGLVKIVI
                     TCKFNVKKVAIDPSLVGEDKDMLEDLVMAAMNDAVAKAEAATQAKMAGLTAGLNLPPG
                     MGLPF"
     gene            2226..>2417
                     /gene="recR"
                     /locus_tag="VF309_02220"
     CDS             2226..>2417
                     /gene="recR"
                     /locus_tag="VF309_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381171.1"
                     /note="involved in a recombinational process of DNA
                     repair, independent of the recBC complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEX7465426.1"
                     /translation="MDKPAPLEELIQSLRCLPGVGPKSAQRMAYHLLQRDQKGAERLA
                     RGLGDALSRIRHCARCNTFT"
BASE COUNT          408 a          803 c          830 g          376 t
ORIGIN      
        1 tacgtgcttc gcggcggaag cttcgccacc gtgccgcgcc tcaggtacgc gcgctaccgc
       61 aatttctacc ttccggggcg cggcgacatg ttcgcgggat ttcgcacgtg cgcgctcgaa
      121 gcgcggcaag gctgtgtcat acttcattcc ggcgggcctc cccgcattga agagcgatga
      181 acctggtcag gtcgggaacg aagcagccat agtcgattcc ttcaagtgcc gggggtcagg
      241 ctcgcctccc ccaatttcat ctcgccgatg acgtatcaag ttctcgcccg caagtggcga
      301 ccccgggttt tcgaggagat cgtcggccag tcgcacgtcg tgacggccct cacgaacgcc
      361 ctcgattcca agcgcctcca ccacgcgtat ctcttcaccg ggacgcgcgg cgtgggcaag
      421 accacgcttg cccgtatcct cgccaaggcg ctcaactgcg agacgaacgg catctcctcg
      481 catccctgcg gaaaatgccg cgcgtgcgtg gacatcgacg cgggacgctt cccggatctc
      541 ctcgaagtcg acgcggccac gaacaccaag gtcgacgaga tgcgcgagct tctcgacacc
      601 gcgcagtaca tgccggtggc cgggcgcttc aaggtctaca tcatcgacga ggtgcacatg
      661 ctgtcgcggc atgcgttcaa ctcgatgctg aagacgctgg aagagccgcc cgagcacgtg
      721 aagttcatcc tcgcgacgac ggatccgcag cgcctgccgg tgacgatcct gtcgcgctgc
      781 ctgcagttca acctgaagcc gctcacgccc gcgctcatcg cggggcacct gaagcatgtg
      841 ctgacggccg agggcatcgc cttcgaggag cccgcgctcg cgcagctcgc gaaggcggcg
      901 tccgggagcc tgcgcgacag cctgagcctg ctcgaccagg cgatcgctca tggcggcggc
      961 aaggtcgccg cggcgcaggt cgcggagatg ctcggctccc tcggcggcga tctcgtgtgg
     1021 cagttgctcg agcgcatcgc cgaaggcgac ggcaagggca cgatcgcgga ggcggagcgc
     1081 atcgcttcgc gaagcctgtc gctcgattcg gcattgggcg atctcgcggc gatcctgcac
     1141 cgcatcgcgc tcgcgcaggc cggggcgatt cccgcggatg acgatcccga tacccagcgc
     1201 atcggcgaca tgtcgcaacg catcgacgcg gggcgggtgc aggtcatgta ccagatcgcg
     1261 ttgctgggcc ggcgcgacct ggcgctcgcg cccgatgagc tcgcgggctt cacgatggcg
     1321 ctattgcgga tgctttcgtt cgcgggcccg gcgaatcagg agaggccccg ttcggaggtc
     1381 aaggttgcat cgccgcgtgc ggcgccggcg gccgcgcctc gcgcaaacgc gccctcgccg
     1441 ccggcgccct ctgttgcgtc gacggggccc gtcgctttcg atggcgactg gcccgcgctg
     1501 gtcgagcgcc tgcgcctcac ggggatggcg ggcatggccg cgcgccacgc cgagctcgat
     1561 tcgttccaga acaaccatct cgagctcgtc gtgccggaga ccgatcgcgt ctatgccgag
     1621 aaggcgtacc aggacaagct gaaggcggat ctcgcggcgc acttcgggcc cggattccgg
     1681 ctgagcgttc gcgtcggcgc cacggcgggc gcgagcgtgg cggcggcgaa gtcgcgcgaa
     1741 accgccctga agcaggcgag cgcggccgag gcgatcgagg aagacccgtt cgtgcgcgac
     1801 ctcgtgcgcg atctcggcgc cgaggtcgtc ccttcctcca tccgtcccgc cgacggcgcg
     1861 ggcaaacaat cgagcgacaa gaggtgactc catgatgaag ggaaatttgg gcgcgctgat
     1921 gaagcaggcg cagcagatgc aggagaacct gaagaaggcc caggaagagc tcgcgaagat
     1981 cgaggtcgag ggccagtcgg gcgcgggcct ggtgaagatc gtgatcacct gcaagttcaa
     2041 cgtgaagaag gtggcgatcg atcccagcct cgtcggcgag gacaaggaca tgctcgagga
     2101 cctggtgatg gccgcgatga acgacgcggt ggcgaaggcc gaggccgcga cgcaggcgaa
     2161 gatggcgggc ctcacggcgg gcctgaacct gccgccggga atgggattgc cgttctaggg
     2221 aatcgatgga caagcccgcg cctctcgagg agctgatcca gtcgttgcga tgcctgcccg
     2281 gcgtgggacc gaagtcggcg cagcgcatgg cctatcacct cctgcagcgc gaccagaagg
     2341 gcgcggagcg gctcgcgcgc ggcctgggcg atgcgctctc ccgcattcgc cactgcgcgc
     2401 gctgcaatac cttcacc
//
LOCUS       DASVII010000171         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_44070, whole
            genome shotgun sequence.
ACCESSION   DASVII010000171 DASVII010000000
VERSION     DASVII010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44070"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VF309_02225"
     CDS             <1..321
                     /locus_tag="VF309_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX7465427.1"
                     /translation="DPAWQRAALSRPLEERVELAKGMRLQSEGAKQGKAMDIMDVSAS
                     AVERAFEESGCDVLIHGHTHRPGRHSYQVGGRERIRWVLPDWYDGGGYLEASPAGLRA
                     IDAV"
     gene            complement(273..1220)
                     /locus_tag="VF309_02230"
     CDS             complement(273..1220)
                     /locus_tag="VF309_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7465428.1"
                     /translation="MNRFRGAILACLALALALAGCDVTPRVAKEREPRFAQPAGPAPR
                     LALVLGSGGPRGFAHIGVLKALDDAGVKPDLIIGSSVGSMVGALYASGMSARDLERLA
                     YEINVLEFFEFRSLRGGMATGGAIQNYVDAKVGGLAIEQLKVPFVAAATRMSDGKMAL
                     FNRGDTGLAVRASGASPGQFEPVRIGDDLYVDGDESSPVPIEAARRLGARVVIAVDVS
                     AYVEDTPNSAPREWIAKDERRARQVAAEAKDADVMIHPNIGYYAGMTEPYRRQVIAIA
                     ERVAREKIPAIQAALARAGVAAPPQTASMARRPAGDASR"
     gene            complement(1220..1663)
                     /locus_tag="VF309_02235"
     CDS             complement(1220..1663)
                     /locus_tag="VF309_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7465429.1"
                     /translation="MRRIALLALVALSLPAAAQVYKWVDEKGVAHYSETPPPDGKAAK
                     IELKPPTGTPPPVPETDWKQKELDARQQRIQKEQKEQQRDAQEHNQAAARYNKCLEAR
                     RRLDIMQRPAAVYHLNEKGEKVYYDDAQREREIAGLQANAKTYCD"
     gene            complement(1660..>2321)
                     /locus_tag="VF309_02240"
     CDS             complement(1660..>2321)
                     /locus_tag="VF309_02240"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX7465430.1"
                     /translation="VRLVLPQPGSPDMVFTANAGLVKSKRFVVSRFRYPERQAEEPYF
                     ADWFMDRGYDVSLMPRDVPFEGAGDALFDRGDGCLWMAFGHRSIPAAREILAERLGLD
                     VVVLKLVDQRFYHLDTCFCPLEGGYVMYYPAAFDDVSRESIERRVAADRRIAVEEKDA
                     LEFACNAVNVDSTVVVNRASTGFVKALARRGFEVVQTPLSEFMKAGGSAKCLTLRLDE
                     P"
BASE COUNT          356 a          828 c          767 g          370 t
ORIGIN      
        1 gatcccgcct ggcaacgcgc cgcgctctcg cgcccgctcg aagagcgcgt cgagctcgcc
       61 aagggcatgc gcctgcagag cgaaggcgcc aagcagggca aggcgatgga catcatggac
      121 gtgtcggcca gcgccgtgga gcgcgccttc gaggagtcgg gctgcgatgt gctgatccac
      181 ggccacacgc atcggcccgg gcggcattcg taccaggtcg gcgggcgcga acgcattcgc
      241 tgggtgctgc ccgattggta cgacggcggc ggctacctcg aagcgtcgcc cgcgggcctg
      301 cgcgccatcg acgcagtctg aggcggcgcg gccacgcccg cgcgcgcaag ggccgcctgg
      361 atcgcgggaa tcttctcgcg cgcgacgcgc tcggcgatcg cgatcacctg ccgccgatac
      421 ggctcggtca tgcccgcgta gtagccgatg ttgggatgga tcatgacatc ggcatccttg
      481 gcctccgccg cgacctggcg cgcgcgccgc tcgtccttgg cgatccactc gcgcggcgcc
      541 gaattcggcg tgtcctcgac gtaggccgac acgtccacgg cgatcaccac cctcgcgccg
      601 agccttcgcg ccgcctcgat cggaacgggg ctcgactcgt cgccatccac gtagaggtca
      661 tcgccgatcc gcaccggctc gaactgtccc gggctagcgc ccgatgcgcg caccgcgagc
      721 ccggtgtcgc cgcggttgaa gagcgccatc ttgccgtcgc tcatgcgcgt ggccgccgcg
      781 acgaatggaa ccttcagctg ttcgatcgcg agcccgccga ccttggcgtc gacgtagttc
      841 tggatggcgc cgccggtggc catcccgccg cgcagcgagc ggaattcgaa gaactcgaga
      901 acgttgattt cgtatgcgag ccgctccagg tcgcgcgcgc tcatgcccga cgcgtaaagc
      961 gcacccacca tggatcccac gctcgagccg atgatgaggt cgggcttcac gccggcatcg
     1021 tcgagcgcct tcagcacgcc gatgtgcgcg aatccgcggg ggccgccgga accgagcacc
     1081 agcgccagcc gcggagcggg gcccgccggt tgcgcgaagc gcggctcccg ttccttcgcg
     1141 acgcgcggcg tgacgtcgca tccggcgagc gcgagggcga gggcgaggca ggcgaggatc
     1201 gcgccccgga aacgattcat cagtcgcagt acgtcttcgc gttcgcctgc aacccggcaa
     1261 tctcgcgttc gcgctgcgcg tcgtcgtaat acaccttctc gcccttttcg ttcaggtggt
     1321 acacggccgc gggcctctgc atgatgtcca accggcgccg cgcctcgagg catttgttgt
     1381 accgcgccgc cgcctggtta tgctcctgcg catcacgctg ctgttccttc tgctccttct
     1441 ggatgcgctg ctgccgggcg tcgagctctt tctgcttcca gtcggtctcc ggcaccggcg
     1501 gcggggttcc ggtcggcggc ttgagctcga tcttcgccgc cttgccgtcg ggcggcggag
     1561 tctccgagta gtgggcgaca cccttctcgt cgacccattt gtacacctgc gccgccgcgg
     1621 ggagcgacag cgcgaccaac gccagcagcg cgatccgtct catggctcgt ccagtctcaa
     1681 ggttaggcac ttggccgatc cgcccgcctt catgaactcg gagagcggcg tttgcacgac
     1741 ctcgaacccc cgccgtgcca gggccttcac gaacccggtc gaagcgcgat tcaccaccac
     1801 cgtcgaatcg acgttcaccg cgttgcacgc gaactcgagc gcatccttct cctcgacggc
     1861 aatgcgcctg tccgcggcga cccgcctctc gatcgattcg cgcgagacat cgtcgaacgc
     1921 cgcggggtag tacatcacgt agccgccctc gagcgggcag aagcaggtgt cgaggtgata
     1981 gaagcgctgg tccaccagct tcagcaccac gacgtcgagg ccgaggcgct cggcgagaat
     2041 ctcgcgcgcc gcgggaatgg agcggtggcc gaacgccatc cacaggcacc cgtcgccacg
     2101 atcgaagagc gcgtctcccg cgccctcgaa gggcacgtcg cgcggcatca gcgagacgtc
     2161 gtagccgcga tccatgaacc agtcggcgaa gtaaggctcc tcggcctggc gctccgggta
     2221 gcggaagcgc gacacgacga agcgcttcga tttcaccagc cccgcgttgg cggtgaacac
     2281 catgtcggga gagccgggct gcggcagcac aaggcgaacg g
//
LOCUS       DASVII010000172         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_295789,
            whole genome shotgun sequence.
ACCESSION   DASVII010000172 DASVII010000000
VERSION     DASVII010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295789"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1421
                     /gene="der"
                     /locus_tag="VF309_02245"
     CDS             27..1421
                     /gene="der"
                     /locus_tag="VF309_02245"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7465431.1"
                     /translation="MKPTVVLVGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGQ
                     GRLGDKQFIVVDTGGFEPVAKDGIMSEMARQAREAIAEADVVLFIVDGRAGLSPQDKR
                     IAQQLRESGASVVLVVNKTEGMRLEMVTAEFHELGLGMPSAISAEHGDGVRSLIDDAL
                     APFPVEEPEEQLQDHPRVAIVGRPNVGKSTLVNRLLGEERMIAFDQPGTTRDAIEVEL
                     EWEGKRYTLVDTAGIRRKGKVFESVEKFSVVKTLQAIERANVVVLVVDAESDIAEQDA
                     HVGGYILESGRSLVVAVNKVDAIDAEKRKEVKTDLERKLFFLDFAEFVPISARMGTGI
                     KPLLKAVDVAFAAAMAKIPTPKLTRALQAAVEQQQPPMSGRFRPKMRYAHQGGQNPPT
                     VVIHGSGLDGVKEPYKRYLESTFRKHFKLKGTPLRIELRSSTNPFEGRHKPLTEKQDA
                     QRRRRKIVSKRRYG"
     gene            1568..>1718
                     /gene="hfq"
                     /locus_tag="VF309_02250"
     CDS             1568..>1718
                     /gene="hfq"
                     /locus_tag="VF309_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX7465432.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNT"
BASE COUNT          353 a          524 c          582 g          259 t
ORIGIN      
        1 tctgtggttc aagacttaag gcgctagtga aacccacggt agtgctggta ggaaggccga
       61 acgtcggcaa gtcgacgctc ttcaaccgcc tcacgcgctc gcgggacgcg ctggtcgcgg
      121 accttccggg cctcacgcgc gaccgccact atggacaggg ccggctgggc gacaagcagt
      181 tcatcgtcgt cgacaccgga ggattcgagc cggtcgcgaa ggacggcatc atgtcggaga
      241 tggcgcgcca ggcgcgggag gcgatcgccg aggccgacgt ggtgctgttc atcgtcgatg
      301 gccgcgccgg gctctcgccg caggacaagc gcatcgcgca gcagcttcgc gaatcgggcg
      361 cctccgtggt cctcgtggtc aacaagacgg agggcatgcg cctcgagatg gtgacggcgg
      421 agttccacga gctcggcctc gggatgccaa gcgccatttc cgcggagcac ggcgatggcg
      481 tgagaagcct catcgacgat gcgctcgcgc cgtttcccgt ggaggagccc gaggagcagc
      541 tgcaggacca cccgcgcgtc gcgatcgtcg gccggcccaa cgtcggcaag tccacgctcg
      601 tgaaccgcct cctcggcgag gagcggatga tcgccttcga ccagccgggc accacccgcg
      661 acgcgatcga ggtcgagctc gagtgggagg gcaagcgcta cacgctcgtc gacaccgcgg
      721 gaataaggcg caagggaaag gtgttcgagt cagtggagaa gttctccgtg gtgaagacgc
      781 tgcaggcgat cgagcgcgcg aacgtggtgg tcctggtggt cgacgccgag agcgacatag
      841 ccgagcagga cgcgcacgtc ggcggctaca tcctcgaatc gggacgctcg ctggtggtcg
      901 cggtcaacaa ggtggatgcc atcgacgccg agaagcgcaa ggaggtgaag acggacctcg
      961 agcgcaagct cttcttcctg gacttcgcgg agttcgttcc gatctcggcg cgcatgggca
     1021 cgggcatcaa gccgctgctg aaggcggtcg acgtcgcgtt cgccgcggcg atggcgaaga
     1081 tcccgacgcc gaagctcacg cgcgcgttgc aggcggccgt ggagcagcag cagccaccga
     1141 tgtcggggcg tttccgcccg aagatgcgct acgcgcacca gggcgggcag aatccgccca
     1201 cggtcgtgat ccacgggtcg gggctggacg gcgtgaagga gccctacaag cgctacctcg
     1261 aaagcacatt tcgcaaacat ttcaagctca agggaacgcc gctgcgcatc gagctgcgct
     1321 cgtcgacgaa tcccttcgag ggccgccaca agcccttgac ggaaaagcag gatgcgcagc
     1381 ggcggcggcg caagatcgtc tccaagcgcc gctacggata ggcgggaaac cgcgcctcag
     1441 gaactcggcc aagagagcgt tgtcccttgg tttggatgga taaaaatgct gctttgcagt
     1501 aaagtccatt tgcaggcaac ggcccggcgt agaccgggaa acccgaagag agaaaagggg
     1561 caaaacaatg agcaacaagg gccaattgct acaagacccg ttcctcaaca cgttgcgcaa
     1621 agagcatgta ccggtctcga tctacctggt gaacggcatc aagctgcagg gccagatcga
     1681 atctttcgac caatacgtcg tgctcctcaa gaacaccg
//
LOCUS       DASVII010000173         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_367928,
            whole genome shotgun sequence.
ACCESSION   DASVII010000173 DASVII010000000
VERSION     DASVII010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367928"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1602
                     /locus_tag="VF309_02255"
     CDS             <1..>1602
                     /locus_tag="VF309_02255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028899.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7465433.1"
                     /translation="LRAYDSRQIEIQASVKVRIKELERSADGAIVEKWTRYDTTVGRA
                     LMSEILPAGLPFSIINKPLKKKEISKLINEAFRRCGIRETVIFADKLMYTGFSFATRA
                     GLSICVDDLLIPAQKAGIIDESENQVKEIERQYTSGLVTQGERYNKVVDIWGRAGDVV
                     AKAMMDQLGNQKVTDRKGKEVNQESFNSIYMMADSGARGSAAQIRQLAGMRGLMAKPD
                     GSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVV
                     IEDDCGTNTGMAMKALVEGGEVVEALRERILGRVTAADVFNTENQELLLPAGSLLDED
                     SVERIEALGIDEVKVRTPLTCDTRYGLCAACYGRDLGRGVRVNVGEAVGVIAAQSIGE
                     PGTQLTMRTFHIGGAASRTAVASSVESKSNGVAHFAPTMRHLTNARGEIIAISRSGEI
                     IISDDTGRERERHKVPYGATLTVADGAKVKAGQSLANWDPLTRPIITEYAGETRFENV
                     EEGVTVAKQIDETTGLSSLVVIDPKR"
BASE COUNT          306 a          525 c          545 g          226 t
ORIGIN      
        1 gctgcgtgct tacgacagcc gtcagatcga gatccaggcg agcgtcaagg tgcgcatcaa
       61 ggaactggag cgtagcgccg acggcgccat agtggaaaag tggacccgtt atgacacgac
      121 ggttggccgc gcgctcatgt cggagatcct gccggcgggg ctgcccttct cgatcatcaa
      181 caagccgctc aagaagaagg agatctccaa gctcatcaac gaggcgttcc gccgctgcgg
      241 aatccgcgag acggtgatct tcgccgacaa gctcatgtac acgggcttca gcttcgccac
      301 gcgcgcgggc ctgtcgatct gcgtcgacga cctgctgatc ccggcccaga aggccggcat
      361 catcgacgag agcgagaacc aggtgaagga gatcgagcgc cagtacacct cgggcctggt
      421 gacccagggc gagcgctaca acaaggtggt ggacatctgg ggccgcgccg gcgatgttgt
      481 cgccaaggcc atgatggacc agctgggcaa ccagaaggtc accgaccgca agggcaagga
      541 agtcaaccag gagtcgttca actccatcta catgatggcg gactcgggcg cgcgcggctc
      601 ggcggcgcag atccggcagc tcgccggcat gcgcggcctc atggcgaagc cggacggctc
      661 catcatcgag acgccgatca cggccaactt ccgcgaaggc ctgaacgtgc tgcagtactt
      721 catctcgacg cacggcgcgc gtaaaggcct ggccgatacc gcgttgaaga cggcgaactc
      781 gggatacctc acgcgccgcc tggtggacgt gacccaggac ctcgtggtca tcgaggacga
      841 ctgcggcacc aacaccggca tggcgatgaa ggcgctggtc gagggcggcg aggttgtcga
      901 ggcgctgcgc gagcgcatcc tcggccgcgt taccgcggcc gacgtgttca acaccgagaa
      961 ccaggagctc ctgctgccgg cgggctcgct cctcgacgag gattcggtcg agaggatcga
     1021 ggccctcggc atcgacgagg tcaaggtgcg cacgccgctc acctgcgaca cgcgctacgg
     1081 cctgtgcgcg gcctgctacg gccgcgacct gggccgcggt gtgcgcgtga acgtgggcga
     1141 ggcggtgggc gtcatcgcgg cccagtccat cggcgagccg ggcacgcagc tcaccatgcg
     1201 gaccttccac atcggcggcg cggcatcgcg caccgcggtg gcgagctcgg tcgagtcgaa
     1261 gtccaacggt gtcgcgcact tcgcgcccac catgcgccac ctcaccaacg cgcgcggcga
     1321 gatcatcgcc atctcacgca gcggcgagat catcatctcg gacgacaccg gtcgcgagcg
     1381 cgagcgccac aaggttccgt acggcgcgac gctcaccgtg gccgacggcg cgaaggtgaa
     1441 ggcgggccag tcgctcgcca actgggatcc gctcacgcgt cccatcatca ccgagtacgc
     1501 gggcgagacg cggttcgaga acgtcgagga aggcgtgacc gtggccaagc agatcgacga
     1561 gaccacgggc ctgtcgagcc tggtcgtgat cgatccgaag cg
//
LOCUS       DASVII010000174         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_403602,
            whole genome shotgun sequence.
ACCESSION   DASVII010000174 DASVII010000000
VERSION     DASVII010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403602"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VF309_02260"
     CDS             complement(<1..886)
                     /locus_tag="VF309_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7465434.1"
                     /translation="MNRKAVLVVDDEADIRELLVLTLSRMGVDADTASTVKEAEEALK
                     LRQYDLALTDMRLPDGDGLMVLRYIAEHHGNTPVAVITAYGSTENAVAALKAGAFDYV
                     AKPIKLEQLRPLVMSALKLPKPVQSRRVATGEVAQPGLPRLLGESPPILRAREMIAKL
                     ARSQAPVYITGESGSGKEVAARLIHSGSSRADAPFVAVNCGAIPENLMESEFFGYKKG
                     AFTGADTDKMGYLKAAEKGTLFLDEVGDLPLGMQVKLLRVIQEKKVRRVGDTVEDPID
                     VRIISATHRNLSEQVDAGQ"
     gene            complement(886..2514)
                     /locus_tag="VF309_02265"
     CDS             complement(886..2514)
                     /locus_tag="VF309_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7465435.1"
                     /translation="MSSVAEPVAIAQAVPSQRSVLIVEADDTTWRTLFVFCAYRVVLA
                     ILVGGAFAFLNRFFNFGIVSPGSVLPTVVSYTIASLVLLVPARLREPSLMLQVTAGVL
                     VDVIAIVMLMYASGGVRSGLGVMLLVSLAAAGLITRGRLAFFHAALAALAILLEQTFQ
                     MWRYDAGMQDFVQAGLTSAAFFATAGLASRLARYARTSEQIAEERKVDLANLSQINEL
                     VIRDMQDGILVVDATGRIRQSNPRAMQLLGAMPQGRWPSLGDYSPEIAGLLEAWRRGS
                     ETTFMQLRGPRSTGELQVHFVPIGVGNPAPTVIFVEDVGRMRQQAQQLKLVALGRLTA
                     SIAHEIRNPLSSIGHAAELLEEDKGLGEPEQRLLEIIRGNVTRLDRIVQEVLYLNRRD
                     RAQPEAIDAFAYLAQFAQEFCATEKAPAGSLDVQVRTSQRLMFDKQHLDQVLWNLARN
                     AWRHSRKLAGSVRIIASPSPVPGRLQIEIIDDGPGPSREAQAHLFEPFFTTEAHGTGL
                     GLYIARELCEGNGARIEYLEGSGHGHFRIILKGA"
     gene            complement(2511..>2782)
                     /locus_tag="VF309_02270"
     CDS             complement(2511..>2782)
                     /locus_tag="VF309_02270"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEX7465436.1"
                     /translation="IAFAVNFAYSGDKNLARIVLLLVIGFVIYLALRGFLRAQARGDE
                     KQPDRAAKGEDMVACARCGVHLPRSEARTEAGIFYCQDNPRCRAP"
BASE COUNT          450 a          927 c          972 g          433 t
ORIGIN      
        1 actggccggc gtcgacctgc tccgagaggt tgcggtgggt ggccgagatg atgcgcacgt
       61 cgatgggatc ctccacggtg tcgccgacgc ggcgcacttt cttctcctgg atcacgcgca
      121 gcagcttcac ctgcatcccg agcggaaggt cgccgacctc gtcgaggaag agcgtgccct
      181 tctccgccgc cttgagatat cccatcttgt ccgtgtcggc gccggtgaat gcccccttct
      241 tgtagccgaa gaactcgctc tccatcaggt tctcgggaat cgcgccgcag ttcaccgcga
      301 caaatggagc atccgcgcgg gaggatcccg aatggatgag ccgcgcggcg acttccttgc
      361 cgctgccgga ttcgcccgtg atgtacaccg gcgcctggga gcgcgcgagc ttcgcgatca
      421 tctctcgcgc gcgcaggatc ggcggggact cgccgagaag gcgcggcaag cccggttgcg
      481 cgacctcgcc cgtggcgacg cgccgcgatt gcacgggctt cggcaacttc aacgcgctca
      541 tcacgagcgg gcgcagctgc tcgagcttga tcggcttggc gacgtagtcg aaggccccgg
      601 ccttcaacgc ggcgaccgcg ttctcggtcg agccgtaggc cgtgatcacg gcgacgggcg
      661 tgttgccatg gtgctcggcg atatagcgaa gcaccatgag gccgtcgccg tcgggaaggc
      721 gcatgtcggt gagcgcgagg tcgtactggc gcagcttcag cgcctcctcg gcctccttca
      781 cggtcgacgc ggtgtcggcg tccacaccca tgcgcgagag cgtgagcacc agcagctcgc
      841 ggatgtcggc ttcgtcatcg acgacgagca cggctttgcg gttcatcaag cgcctttcag
      901 gatgatgcgg aagtgtccat ggccggaccc ctcgaggtat tcgatgcggg cgccgttgcc
      961 ttcgcaaagc tcgcgggcga tgtagaggcc gaggcccgtg ccgtgcgcct cggtcgtgaa
     1021 gaacggctcg aagaggtgcg cctgcgcttc gcgcgaaggc ccgggcccgt cgtcgatgat
     1081 ctcgatctgc aggcgccctg gcaccggcga cggcgaggcg atgatgcgca ccgaccccgc
     1141 gagcttgcgg ctatggcgcc aggcgttgcg cgcgaggttc cacaggacct ggtcgaggtg
     1201 ctgcttgtcg aacatgaggc gctgcgaggt gcgcacctgc acgtcgaggc tgccggcggg
     1261 cgccttctcc gtggcgcaga actcctgggc gaactgggca aggtacgcga atgcgtcgat
     1321 cgcctcgggc tgcgcgcggt cgcggcggtt gaggtagagc acctcctgca cgatgcgatc
     1381 cagccgcgtc acgttgccgc ggatgatctc gaggagccgc tgctccggct cgcccaggcc
     1441 cttgtcctcc tcgagaagct ccgccgcgtg gccgatggag gaaagcgggt tgcggatctc
     1501 gtgcgcgatc gaggcggtga ggcgccccag cgcgacgagc ttcagttgct gcgcctgctg
     1561 gcgcatgcgc cccacgtcct cgacgaagat caccgtgggc gccgggttgc cgacgccgat
     1621 cggcacgaag tgcacctgca gctcgcccgt cgagcgcggg ccgcgaagct gcatgaacgt
     1681 ggtctccgat ccgcgccgcc acgcctcgag aagacccgcg atctcgggag agtagtcgcc
     1741 cagcgagggc cagcggccct gcggcatcgc tcccaatagc tgcatggccc gcgggttgct
     1801 ctggcggatc cgtcccgtgg cgtccaccac gaggattccg tcctgcatgt cgcggatcac
     1861 gagctcgttg atctgcgaaa gattggcgag gtcgaccttg cgctcctcgg cgatctgctc
     1921 gctggtgcgc gcgtaacggg ccagtcgcga ggcgagcccc gcggtcgcga agaatgccgc
     1981 gctggtgagc cccgcctgca cgaagtcctg catgcccgcg tcgtagcgcc acatctggaa
     2041 cgtctgctcg agcaggatcg cgagcgccgc gagcgccgcg tggaagaagg cgaggcggcc
     2101 ccgggtgatg aggccggcgg cggcgagcga cacgaggagc atcaccccga gcccgctgcg
     2161 cacgcccccg ctcgcgtaca tcagcatcac gatcgcgatc acgtcgacca ggacgcccgc
     2221 ggtcacctgc agcatgaggc tcggctcgcg aaggcgcgcc ggaacgagca gcacgaggga
     2281 ggcgatcgtg tacgagacca cggtgggcag gacggagccc ggcgagacga tcccgaagtt
     2341 gaagaagcga ttcaggaagg cgaacgcgcc gcccacgagg atcgcgagca cgacccggta
     2401 ggcgcagaag acgaagagcg tgcgccatgt ggtgtcgtcg gcctcgacga tgagcaccga
     2461 ccgctgcgac ggcacggcct gggcgatggc gaccggctcg gccacggagc tcatggcgcg
     2521 cggcagcgcg ggttgtcctg gcaatagaag attccggcct cggtgcgcgc ctcgctgcgc
     2581 ggcaagtgca cgccgcaacg cgcgcacgcg accatatcct cgcccttcgc cgcgcgatcg
     2641 ggctgcttct catcaccgcg cgcctgcgcg cgaaggaacc cgcgcaacgc gaggtagatg
     2701 acgaatccga tgacgagcag caggacgatg cgggccaagt tcttgtcccc cgaatacgcg
     2761 aagtttacgg caaaggcaat tc
//
LOCUS       DASVII010000175         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_547717,
            whole genome shotgun sequence.
ACCESSION   DASVII010000175 DASVII010000000
VERSION     DASVII010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547717"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VF309_02275"
     CDS             <1..791
                     /locus_tag="VF309_02275"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465437.1"
                     /translation="IFVHRMQEETGATPEEVTRAYILTRDIFGLDALWTEIDALDNKG
                     PALLQYQMLIDMGRLVVRSTLWFLRRRREKLPIAQVIDIFGAGLVALRELLPDVLSPA
                     DRVAFDASVTRLMGEGVPPELAGRFAELEGLYAVLDATEVAVEQKKAIELVGALYFAL
                     VGELDLRWIGEKITQLPTDTSWQALARNALRDDLASQQRALTSTVARLSPEASDPARM
                     LLAWKERYSPAITRYRNMVEELKRLPSLDLAVLSVLLRELRTLA"
     gene            complement(798..1169)
                     /locus_tag="VF309_02280"
     CDS             complement(798..1169)
                     /locus_tag="VF309_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX7465438.1"
                     /translation="MKIERDEAQGRNTFTGYGEGYVEVNLKRYPSSLVVAGDRLITSW
                     PAASLESLTADHLAAIVQLAPEIVLLGTGSAFSFPEPQLLAPLRKAGIGVEVMDTPAA
                     CRTYNILLGEGRNVVAALIVP"
     gene            1211..>1888
                     /locus_tag="VF309_02285"
     CDS             1211..>1888
                     /locus_tag="VF309_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7465439.1"
                     /translation="MNLNDASKPMIPEFSRLKRLPPYVFSITNELKMAARRAGEDIID
                     LSMGNPDGPTPRHIVDKLVEVAQREDTHGYSVSKGIPKLRRAICQWYKRRWDVDLDMD
                     REAIVTIGSKEGLAHLMLATLDRGDTVLVPNPSYPIHIYGAIIAGADIRSIRMTPGVD
                     FFEEAERAIRELFPKPKMMIIGFPSNPTAMCVELDFFERVIALAKQYGILVVHDLAYA
                     DITFDGWK"
BASE COUNT          314 a          646 c          619 g          309 t
ORIGIN      
        1 gcatcttcgt gcaccgcatg caggaggaga ccggcgcgac tcccgaggag gtgacgcgcg
       61 cctacatcct cacgcgcgac atcttcgggc tcgatgcgct gtggacggag atcgatgccc
      121 tcgacaacaa gggccccgcg ctgctccagt accagatgct gatcgacatg ggccggctcg
      181 tggtgcgctc gacgctgtgg ttccttcgcc ggcgccgcga gaagctgccc atcgcgcagg
      241 tgatcgacat cttcggcgcg gggctcgtgg cgctgcgcga gctgctgccc gatgtcctct
      301 cgcccgccga tcgcgtcgcc ttcgacgcct cggtgacgcg cctcatgggc gagggcgtgc
      361 cgcccgagct cgccggacga ttcgccgagc tcgaggggct ctacgcggtc ctcgacgcga
      421 cggaggtggc cgtcgagcag aagaaggcga tcgagctcgt cggggcgctc tacttcgccc
      481 tcgtgggcga gctcgacctt cgctggatcg gggagaagat cacccagctg cctaccgaca
      541 cctcgtggca ggcgcttgcg aggaacgccc tgcgcgacga cctcgcgagc cagcaacgcg
      601 ccctcacgtc gacggtcgcc aggctttcgc ccgaggcgag cgatccggcg cgaatgctcc
      661 ttgcgtggaa ggaacgctat tcgcccgcga tcacccgcta ccggaacatg gtcgaggagc
      721 tgaagcgcct gccctcgctc gacctcgccg tcctctcggt cctgctgcgc gagctacgca
      781 ctctcgccta ggcgcgctca ggggacgatg agggcggcaa cgacattgcg gccctcgccg
      841 aggaggatgt tgtaggtgcg gcaggcggcg ggggtgtcca tcacctcgac gccgatcccg
      901 gccttgcgca gcggcgcgag aagctggggc tcggggaagc tgaaggcgct tcccgtcccg
      961 agaaggacga tctccggggc gagctgcacg atcgccgcga ggtggtccgc ggtgagcgat
     1021 tcgagcgagg ccgccggcca gctggtgatc agccgatcgc ccgcgaccac gaggctcgac
     1081 gggtagcgct tgaggttcac ctcgacgtat ccctcgccgt atccggtgaa cgtgttgcgg
     1141 ccctgggcct cgtcgcgctc gattttcacg ctagaatcat agcgtcttgc agcgccgcat
     1201 cacaacgaaa atgaacctga acgacgcctc gaagcccatg attcccgaat tctcgcgctt
     1261 gaagcgcctg cccccgtacg tcttcagcat caccaacgag ctgaagatgg ccgcgcggcg
     1321 cgcgggcgag gacatcatcg acctgtcgat gggcaatccc gacgggccca cgccgcgcca
     1381 catcgtcgac aagctggtgg aggtcgccca gcgcgaggac acccacggct actcggtttc
     1441 gaaggggatc ccgaaactgc gccgcgccat ctgccagtgg tacaagcgcc gctgggacgt
     1501 ggacctcgat atggatcgcg aggcgatcgt gaccatcggg tcgaaggagg gcctcgcgca
     1561 cctgatgctc gcgaccctcg accgcggcga cacggtgctc gtgcccaatc cgagctatcc
     1621 gatccacatc tacggggcga tcatcgccgg cgccgacatc cgctcgattc gcatgacgcc
     1681 cggcgtggac ttcttcgagg aggccgagcg cgcgatacgc gagctcttcc ccaagccgaa
     1741 gatgatgatc atcggctttc cgtcgaaccc gaccgccatg tgcgtcgagc tcgatttctt
     1801 cgagagggtg atcgcgctcg ccaagcagta cggcatcctc gtggtccacg accttgccta
     1861 cgccgacatc accttcgacg gctggaag
//
LOCUS       DASVII010000176         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_151912,
            whole genome shotgun sequence.
ACCESSION   DASVII010000176 DASVII010000000
VERSION     DASVII010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151912"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF309_02290"
     CDS             <1..365
                     /locus_tag="VF309_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX7465440.1"
                     /translation="HRVRTEFWGYAAGESLSSDEMIAEKYAGIRPAPGYPACPDHTEK
                     GDLFRLLDAGKNADMRLTESFAMLPTAAVSGFYFSHPQARYFAVGKIERDQVADYAHR
                     KGMDLAQAERWLAPALNY"
     gene            complement(367..>1534)
                     /locus_tag="VF309_02295"
     CDS             complement(367..>1534)
                     /locus_tag="VF309_02295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX7465441.1"
                     /translation="SLAPPAATRSSRASAADDSSEGGFWPSALKYAIPVAVVVLAIWG
                     WKRHKAPPPGPIATVVSVTRIETPADPVPGPSAIPEGTRGDAQTLGSARNAENIFAEI
                     SPSIVSVHSADGSGRQTKQGSGVVTGSGRVITNCHVVSGADQITVASAGETRSASLYV
                     SDEEFDLCSLDVTGLNAPAVTIRGIGGLRTGQRVFAIGAPLGLELTISEGIVSSLRDT
                     ASGKVIQTTAPVSPGSSGGGLFDVDGKLVGIITFQQRSGQNLNFAVPADWIFEMRNRS
                     SSSSRASDAPAAPPSAPAQTAEPTVPQMVVGRWWCFGSLSGRNGEYNYGPDGILRIAS
                     SDGTKGEGPYTVSGRRILYQGANEVFAFDIDSISAERMVQVVGEGRRLVCERRG"
BASE COUNT          241 a          542 c          526 g          225 t
ORIGIN      
        1 accatcgcgt gcgcaccgaa ttctggggat acgcggccgg cgagagcctt tcgagcgacg
       61 agatgatcgc ggagaagtac gcgggaatcc gcccggcgcc cggatacccg gcatgccccg
      121 accacacgga aaagggcgac ctcttcaggc tgctcgacgc ggggaagaat gccgacatgc
      181 ggctcaccga gtccttcgcg atgctgccca cggccgcggt gagcggcttc tacttctccc
      241 atccccaggc gcgctacttc gcggtcggca agatcgagcg cgaccaggtc gccgactacg
      301 cgcatcgcaa ggggatggac ctcgcgcagg ccgagcgctg gctcgcgccc gcgctcaact
      361 actgattcag ccgcgccgct cgcacacgag gcggcgtccc tcgcccacca cctgcaccat
      421 gcgctccgcc gagatggagt cgatgtcgaa ggcgaacact tcattcgcgc cctggtagag
      481 gatgcgccgg ccggagacgg tgtaggggcc ctcccccttg gtgccgtccg aggaggcgat
      541 tcgcaggatc ccgtcggggc cgtagttgta ctcgccgttg cgccccgaga gcgatccgaa
      601 gcaccaccac cggcccacca ccatctgcgg caccgtcggc tcggccgttt gcgccggcgc
      661 gctcggcggc gcagcggggg cgtcgctcgc acgcgatgac gaggagctgc gattgcgcat
      721 ctcgaagatc cagtcggccg gaaccgcgaa attcaggttc tggccggagc gctgctggaa
      781 ggtgatgatg ccgacgagct tcccgtcgac atcgaagagg cccccgccgc tcgatcccgg
      841 cgagaccggc gccgtcgtct ggatcacctt gcccgaggcc gtgtcgcgca acgacgagac
      901 gattccctcc gagatcgtga gctccaggcc gagaggggcg ccgatggcga agacgcgctg
      961 cccggtgcga agtcctccga tgccgcggat cgtcaccgcc ggagcgttga gcccggtgac
     1021 gtcgaggctg cacaggtcga actcctcgtc cgagacatac agcgacgcgg atcgcgtctc
     1081 cccggcgctc gcgaccgtga tctggtcggc gccgctgacg acgtggcagt tggtgatgac
     1141 gcggcccgac cccgtgacga cgccgctgcc ctgcttggtc tggcgcccgc tcccatcggc
     1201 ggaatgcacg ctcacgatgc tcggggatat ttccgcgaaa atgttctcgg cgttgcgcgc
     1261 ggagccgagc gtctgcgcgt cgccgcgcgt gccctcgggg atcgcgctcg ggccggggac
     1321 gggatccgcg ggcgtctcga tgcgggtgac cgacacgacc gtggcgatcg gtcccggggg
     1381 cggagccttg tggcgcttcc atccccagat cgcgagcacc acgacggcca ccggaatggc
     1441 gtacttgagt gccgaaggcc agaatccgcc ctcgctcgag tcatccgccg ccgaggcccg
     1501 cgaagaccgc gtcgccgcgg gcggcgccag cgac
//
LOCUS       DASVII010000177         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_8593, whole
            genome shotgun sequence.
ACCESSION   DASVII010000177 DASVII010000000
VERSION     DASVII010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8593"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VF309_02300"
     CDS             <1..517
                     /locus_tag="VF309_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7465442.1"
                     /translation="IAVRGEAAPAPAPLAPLAARAPSKASSGVLVLGVNNIATLVAKC
                     CKPVPPDPIIGFITRTRGVMVHRLDCPNITGLAEGQRDRLMPADWGKESAAAFAAEIE
                     VVAVDRQGLLRDISEAIARERINVTAVNTLSRGAHAFMRFTLQVASADAIQRVLRQVK
                     DVPGVDTARRL"
     gene            554..>1642
                     /locus_tag="VF309_02305"
     CDS             554..>1642
                     /locus_tag="VF309_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7465443.1"
                     /translation="MKIATLKDLESRLHEPLPVHERVRLLNEISEQLYEREPARGAAL
                     AREAIELARATGDTPGEAQALYCLGRNLYSQADYPAVLETQGTALSLFSAMGDAEGEA
                     RCCNLLGITHRQLADYGRALEMYDAALKGFRDAGDLKWQARVISNIGAVEIQLGNHTA
                     ALELFDQALDLRRETDDNEGAGFDLNNAAFGHVQRALQHRAAQDEVSCRAEAQNALRL
                     LDRALAIARQYGYKRLEAFCLQTMGEAYQAMSRPAVALGLADQFLKLAHDSDDKWIEA
                     YGLACIGEIRHQLGQHADGVALLKRAQHMFEALGSRDETARVLRNLSQAHEAAGELAE
                     ALACLRRAGMIEQSLKSEDTERRARALSA"
BASE COUNT          253 a          572 c          595 g          222 t
ORIGIN      
        1 gatcgcggtg cgcggcgagg ccgctcccgc gcccgcgccg ctcgcgcccc tggccgctcg
       61 cgcgccctcg aaggcttcgt cgggggtgct ggtcctcggc gtgaacaaca tcgcgacgct
      121 ggtggccaag tgctgcaagc cggtgccgcc cgatccgatc atcggcttca tcacccgcac
      181 gcgcggcgtg atggtgcatc ggctcgattg cccgaacatc acggggctcg cggagggcca
      241 gcgcgaccgc ctcatgcccg cggactgggg caaggagagc gccgcggcgt tcgccgccga
      301 gatcgaggtg gtcgcggtcg accgccaggg gttgctgcgc gacatctcgg aggcgatcgc
      361 gcgcgagcgc atcaacgtga ccgcggtgaa cacgctctcg cgcggcgccc acgcgttcat
      421 gcgcttcacg ctgcaggtcg cctcggccga cgcgatccag cgcgtgctgc ggcaagtgaa
      481 ggacgttccc ggcgtggaca cggcgaggcg gttgtgaaga tcgccagccg tcgatgaaga
      541 tcgcgtcccg tccatgaaga tcgctacgct caaggacctc gaaagccggc tgcacgagcc
      601 gttgccggtg cacgagcgcg tgcggctgct gaacgagatt tccgagcagc tctacgagcg
      661 cgagccggcg cgcggcgctg cgctcgcgcg cgaggcgatc gagctcgcgc gcgcgaccgg
      721 ggacacgccg ggcgaagccc aggcgctcta ttgcctcggg cgcaatctct attcgcaggc
      781 ggactacccc gcggtgctcg agacgcaagg cacggcgctg tcgctcttca gcgccatggg
      841 cgacgccgaa ggcgaggcgc gctgctgcaa tctcctcggc atcacccatc ggcagcttgc
      901 cgattacggg cgcgcgctcg agatgtacga cgccgcgctg aaggggttcc gcgacgcggg
      961 cgacctcaag tggcaggcgc gcgtgatctc caacatcggg gccgtcgaga tccagctggg
     1021 caaccacacg gcggcgctcg agcttttcga ccaggcgctc gacctgaggc gcgagaccga
     1081 cgacaacgag ggcgcgggct tcgacctcaa caacgccgcg ttcgggcacg tgcagcgcgc
     1141 gctgcagcat cgcgccgcgc aggacgaggt gagctgccgg gcggaagcgc agaacgcgct
     1201 gcgccttctc gatcgcgccc tcgcgatcgc gcggcaatac ggctacaagc gcctcgaggc
     1261 gttctgcctg cagacgatgg gcgaggcgta ccaggcgatg tcgcggcccg cggtggcgct
     1321 ggggctcgcg gaccagttcc tcaagctcgc gcacgattcc gacgacaagt ggatcgaggc
     1381 gtacggcctc gcgtgcatcg gcgagatccg ccaccagctc ggccagcatg ccgacggcgt
     1441 cgcgctcctg aagcgggcgc agcacatgtt cgaggcgctg ggctcgcgtg acgagaccgc
     1501 ccgggtgctg cgcaaccttt cgcaggccca cgaagccgcg ggcgagctcg ccgaggcgct
     1561 cgcgtgcctg cggcgcgccg gcatgatcga gcagagcctg aagagcgagg acaccgagcg
     1621 gcgcgcgcgg gcgctctcgg cg
//
LOCUS       DASVII010000178         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_224335,
            whole genome shotgun sequence.
ACCESSION   DASVII010000178 DASVII010000000
VERSION     DASVII010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224335"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>2300
                     /locus_tag="VF309_02310"
     CDS             12..>2300
                     /locus_tag="VF309_02310"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF003467.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX7465444.1"
                     /translation="MTHLYPSENPSDSEHMAIVATGGYGRGLLAPGSDIDLLFLLPYK
                     QTAWGESVAEGILYCLWDMGLKVGHATRSVDECIRQAKADMTIRTALLEARYLLGDRA
                     LFDEFVTQFDNKVVRGSAAQFVAAKLAEREERLRRAGQSRYLVEPNVKEGKGGLRDLH
                     TLFWIAKYVYRVREPEELIAHGVFDREEYKQFRRAEDFLWAVRCHMHFVMKRAEERLA
                     FDIQREIAVRLGYTAHPGMRDVERFMKHYFLVAKDVGDLTAILCAGLEEREAKSMPVL
                     DRVIARFKPRPRRVVLDSDDFTVDHNRISISDPDAFRRDPVNLIRLFRLAQKHNLAFH
                     PEAIRAAKRSLKLIDQKLREDKIANGLFFEILTSEGAEVVLRRMNEAGVLGRFVRAFG
                     RIVAMMQFNMYHHYTVDEHLLRCIGILADIDAGGNDEFVLASELMRTIQKPHRALLYV
                     ALFLHDIAKGRVEDHSIAGARVARRFCPRLGLSAAETDTVAWLIEQHLVMSSIAQSRD
                     LSDRKTIENFANVVQSLERMKLLLILTTADIRAVGPGVWNSWKAQLLRTLYYETEPVL
                     TGGFSEVNRQKRVAMAQAEFRAEMKDWGKEALDAYITRHYPAYWLKVDLPHKVSHANF
                     LRSAQAAGKAVATGVGFDTARGVTELTVLAPDHPRLLSVIAGACAAAGANIVDAQIFT
                     TTDGLALDTIAVSREFERNEDEARRAVRIADSIEKALRGELRLPDVMAKRTARKARLK
                     AFAIEPEVMLNNQWSNRYTVVEV"
BASE COUNT          401 a          737 c          757 g          405 t
ORIGIN      
        1 tcgagtttgt cgtgacacat ctctacccgt cggaaaatcc ttccgactcc gagcacatgg
       61 cgatcgtggc gaccggcggc tacggccgcg gcctgctcgc gcccggctcc gacatcgatc
      121 tgctttttct cctgccctat aagcagaccg cgtggggcga gtcggtcgcc gaaggcattc
      181 tttattgcct ctgggacatg gggctgaagg tcggccacgc gacgcgctcg gtcgacgaat
      241 gcatccgcca ggccaaggcg gacatgacca tccgcaccgc gctgctcgag gcgcgctatc
      301 tgctcggcga tcgcgcgctg ttcgacgaat tcgtcacgca gttcgacaac aaggtggtgc
      361 gcgggagcgc ggcgcaattc gtcgccgcca agctcgccga gcgcgaggaa cggctgcgcc
      421 gagccggaca gtcgcgctat ctggtcgagc ccaacgtcaa ggaaggcaag gggggcttac
      481 gcgatctgca cacgctgttc tggatcgcga aatatgtcta ccgcgtgcgc gagcccgaag
      541 agctgatcgc gcacggcgtg ttcgaccgcg aggaatacaa gcagttccgc cgcgcggagg
      601 attttctctg ggcggtgcgc tgccacatgc acttcgtgat gaagcgcgcc gaggagcggc
      661 tcgctttcga catccagcgc gaaatcgcgg tccgcctcgg ctacacggcg catcccggca
      721 tgcgcgacgt cgagcgcttc atgaagcact atttcctggt cgccaaggat gtcggcgatc
      781 tgaccgcgat cctgtgcgcg gggctggagg agcgcgaggc aaagtccatg ccggtgctcg
      841 accgcgtgat cgcgcgcttc aagccgcgtc cgcgccgcgt ggtgctcgat tcggacgatt
      901 tcacggtcga ccacaatcgc atcagcattt cggaccctga cgcgttccgg cgtgatccgg
      961 tcaacctgat ccgtctgttt cgactggcgc agaagcacaa tctggcgttc cacccggaag
     1021 cgatccgcgc cgcgaagcgc tcgctcaagc tgatcgacca gaagctgcgc gaggacaaga
     1081 tcgcgaacgg cctgttcttc gagatcctta ccagcgaggg cgccgaggtc gtgctgcgcc
     1141 gcatgaacga ggcgggcgtg ctcggccgtt tcgtgcgcgc cttcgggcgc attgtcgcga
     1201 tgatgcagtt caacatgtat caccactaca cggtggatga gcatctgttg cgctgcatcg
     1261 gcatccttgc cgacatcgac gcgggtggaa acgacgagtt cgtgctcgcg agcgagctga
     1321 tgcgcacgat ccagaagcca catcgtgcgc tgctttacgt cgcgctgttc ctgcacgaca
     1381 ttgccaaggg acgcgtggag gaccattcga tcgcgggcgc gcgcgtcgcg cgtcgcttct
     1441 gcccgcggct cggcctctcg gcggccgaga ccgatacggt tgcctggctg atcgagcagc
     1501 acctggtgat gtcgtcgatc gcgcaatcgc gcgatctctc agaccgcaag acgatcgaga
     1561 attttgcaaa tgtcgtgcaa tcgctcgagc gcatgaagct gctgctgatc ctcaccacgg
     1621 cggatattcg cgcggttggc cccggcgtgt ggaattcctg gaaggcgcag ctcttgcgca
     1681 cgctctatta cgagaccgag ccggtgctga ccggcggttt ctcggaagtg aaccggcaga
     1741 agcgggtcgc gatggcgcag gccgagttcc gcgccgagat gaaggactgg ggcaaggagg
     1801 cgctcgacgc ctacatcacg cgtcactacc cggcctactg gctcaaggtc gacctgccgc
     1861 acaaggtcag ccacgcgaac ttcctgcgct ccgcgcaggc cgccggcaag gcggtcgcga
     1921 ccggcgtcgg gttcgacacg gcgcgcggcg tcaccgaatt gaccgtgctg gcgcccgatc
     1981 atccgcggct gttgtcggtg atcgcggggg cgtgcgcggc ggcgggcgcc aacatcgtcg
     2041 atgcgcagat cttcaccacc accgacgggc ttgcgctcga caccatcgcg gtgtcgcgcg
     2101 agttcgagcg caacgaggac gaggcgcgcc gcgcggtgcg catcgcggat tcaatcgaga
     2161 aggcgctgcg cggcgaattg cgcctgcccg acgtgatggc aaagcgcacc gcgcgcaagg
     2221 cgcgcctgaa agccttcgcg atcgagccgg aggtgatgct caacaaccag tggtccaatc
     2281 gctatacggt cgtcgaagtc
//
LOCUS       DASVII010000179         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_116634,
            whole genome shotgun sequence.
ACCESSION   DASVII010000179 DASVII010000000
VERSION     DASVII010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116634"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1683
                     /gene="pheT"
                     /locus_tag="VF309_02315"
     CDS             <1..>1683
                     /gene="pheT"
                     /locus_tag="VF309_02315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7465445.1"
                     /translation="EVLGLDDVYFTVKLTPNRGDCLSMFGIARDLAAMVKVAFEPPAA
                     PVVAVSSDDSRPVGISEPNACGAYFGRVIRGIDPAAKTPAWMARRLERAGLRVIRPLV
                     DITNYVMIERGQPMHAFDNAKLQGAIGVRLMQPGERVRLLNEQEVEYRPGLLAITDER
                     GPVALGGVMGGADTMVGDATTEVFLEAAFFHPEAVQGKARDLGLTSDAAYRYERGVDF
                     GATRAALERATELALSICGGSAGPVTEAKGNIPARKATRVRTARVVELLGYPIADGEM
                     AGILRSLSCAVAVESNAMSATPPTWRFDLEIEEDFVEEIARIHGYEHVPAEAPRSRLA
                     MLPLREGERDRFDLRHSLAALGYQEVVNYSFVPPEWEADFAGNSSPVRLANPIASQMS
                     VMRSSLIGGLVQSLRSNLNRGEERVRVFEIGRCFEGIQPDLAVQPERIAGLAFGLRWP
                     EQWGEKGTLVDFFDAKGDLQALAAGRRLDFVPGSHPAFHPGRCALVRASGEALGAIGE
                     MHPRLQQKYELPSPPVMFEVATGPLLQGDFPRFAGLSRMPVVRRDLALVVDD"
BASE COUNT          249 a          567 c          597 g          270 t
ORIGIN      
        1 gcgaggttct cgggctcgac gacgtttact tcaccgtgaa gctcacgccc aaccgcggcg
       61 attgcctctc catgttcggg atcgcccgcg atctcgcggc gatggtgaag gtggcgttcg
      121 agccgcccgc ggcgcccgtc gtcgccgtct cgtcggacga ttcgcgcccc gtgggcattt
      181 ccgagccgaa cgcgtgcggc gcatatttcg gtcgcgtgat ccgcggcatc gatcccgcgg
      241 cgaagacgcc ggcgtggatg gcgcggcgac tcgagcgcgc cggcttgcga gtcatccggc
      301 cgctcgtgga catcaccaac tacgtgatga tcgagcgcgg ccagccgatg cacgcgttcg
      361 acaacgcgaa gctgcaggga gcgatcggcg tgcgcctgat gcagccgggc gaacgcgtga
      421 ggctcctcaa cgagcaggag gtcgaatatc gccccggtct tctcgccatc accgatgaga
      481 gaggtccggt cgcgctcgga ggggtgatgg gcggcgccga cacgatggtc ggcgacgcga
      541 ccacggaggt cttcctcgag gcagcgtttt tccatcccga ggcggtgcag ggcaaggcgc
      601 gcgaccttgg gctcacgagc gacgcggcct atcgctacga gcggggcgtt gatttcggcg
      661 cgacgcgcgc cgcgctcgag cgcgcgaccg aactcgcgct ttcgatctgc ggcggatcgg
      721 cgggcccggt gaccgaggcg aagggaaaca ttcccgctcg caaggccaca cgcgtgcgca
      781 ccgcgcgcgt cgtcgagctg ctcggctatc ccatcgcgga cggcgaaatg gccggcatcc
      841 tgcgaagcct ctcatgcgcc gtcgcggtcg agtcgaacgc gatgagcgcg acgccgccca
      901 cgtggcgatt cgatctcgag atcgaggagg atttcgtcga agagatcgcg cgcatccacg
      961 gctacgagca cgtgcctgcc gaggcgccgc gctcgcgcct agcgatgctg cccctgcgcg
     1021 aaggcgagcg cgatcgcttc gacctgcgcc attcgctcgc ggctctggga taccaggagg
     1081 tcgtgaacta cagcttcgtg ccgcccgaat gggaagcgga cttcgccggc aacagcagcc
     1141 cggtgcgcct cgccaacccc atcgcgagcc agatgagcgt catgcgctcg agcctgatcg
     1201 gcggcctggt gcaaagcctg cgctccaacc tgaatcgcgg cgaggagcgc gtgcgggtct
     1261 tcgagatcgg ccgctgcttc gaaggaatcc agcccgacct cgcggtccag cccgagcgca
     1321 tcgcggggct tgcgttcggc cttcgctggc ccgagcagtg gggcgagaag gggaccctgg
     1381 tcgacttctt cgacgccaag ggcgacctcc aggcgcttgc agccgggcgc aggctcgatt
     1441 tcgtgccggg gtcgcacccg gccttccacc cgggccgctg cgcccttgtc agggcgtcgg
     1501 gagaggcgct gggcgcgatc ggcgagatgc atccgcggct gcagcagaag tacgagctgc
     1561 cttccccgcc ggtcatgttc gaggtggcca cggggccgct gctgcagggt gatttcccgc
     1621 gtttcgcggg cctgtcgcgg atgcccgtgg tgcgtcgcga cctggctctc gtcgtggacg
     1681 acg
//
LOCUS       DASVII010000180         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_44706, whole
            genome shotgun sequence.
ACCESSION   DASVII010000180 DASVII010000000
VERSION     DASVII010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44706"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VF309_02320"
     CDS             <1..1091
                     /locus_tag="VF309_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7465446.1"
                     /translation="QKFDKPGKSPYMDMELVPVYGDDGNAAAGVNVSAQLAQNLGVRT
                     AVAEMASLESSVEAVGAIAFDERSVAIVQSRTAGYVEELFVRAPLDPVRQGQPLLRLF
                     VPDWAGAQHEYLALKSGGSEGSADLARAARNRLALLGMSEAQIAAVDRDGVPVTHVTL
                     AAPIDGVVAELGARQGMNVMPGATLFRINGLGSVWVNVEVPETQAAIVRAGEAVVATV
                     PAYPGERFPGRISAVLPEVNAATRTVRARVELANPGAKLKPGMFATVAIAPKKSREAV
                     LVPTEAVIVTGERSVVVVDRGEGRFEPVDAVIGREEHGRTEILKGVEAGARVVVSGQF
                     LIDSEASLRGAGRRMSPEAPIAPAGEKK"
     gene            1088..>1874
                     /locus_tag="VF309_02325"
     CDS             1088..>1874
                     /locus_tag="VF309_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7465447.1"
                     /translation="MIARLIRWSIANRFLVLLASVGVAAWGAWSMARTPLDALPDLSD
                     VQVIIRTPYPGQAPRIVEDQVTYPLTTTMMSVPGAKAVRGYSFFGDSFVYVLFEDGTD
                     LYWARSRVLEYLNQVQSRLPAQAKASLGPDATGVGWVYEYALVDRTGRHDLGELRAIQ
                     DWFLKYELKSVPDVAEIATVGGMVREYQVVLDPARLRAYGISHGRVVAAIEGANREAG
                     GSVLEMGEAEYMVRSRGYLKTLEDFRSIPLMVGESSTPVTLGDV"
BASE COUNT          280 a          631 c          680 g          283 t
ORIGIN      
        1 agcagaagtt cgacaagccc gggaagtcgc cctacatgga catggagctc gtgccggtct
       61 acggcgacga cggcaacgcc gcggcgggag tcaacgtgag cgcgcagctc gcgcagaacc
      121 tcggcgtgcg aaccgccgtc gccgaaatgg cgagcctcga atcctccgtg gaggcggtgg
      181 gcgcgatcgc gttcgacgag cgctcggtcg cgatcgtgca atcgcgcacc gcgggctacg
      241 tggaagagct cttcgtgcgc gcgcccctcg atcccgtgcg ccaggggcag ccgctcctgc
      301 ggctcttcgt gccggattgg gccggggccc agcatgaata tctcgcgctg aaatccggcg
      361 gctcggaagg atcggccgat ctcgcgcgcg ccgcgcgaaa ccgcctcgcg ctcctcggca
      421 tgagcgaggc gcagatcgcg gccgtggacc gcgacggcgt gcccgtgacc cacgtcaccc
      481 tcgccgcgcc catcgacggc gtcgtcgccg agctgggagc gcgccaggga atgaacgtga
      541 tgccgggcgc gacgctcttc cgcatcaacg gcctcggcag cgtgtgggtc aacgtcgagg
      601 tgcccgagac gcaggcggcg atcgtgcgcg cgggcgaagc cgtggtggcg acggtgcccg
      661 cgtatcccgg cgagcgcttc cccggacgaa tctccgcggt gcttcccgag gtgaacgcgg
      721 ccacgcgaac cgttcgggcg cgcgtcgagc tcgcgaatcc cggggcgaag ctcaagcccg
      781 gcatgttcgc gacggtcgcg atcgcgccga agaagtcgcg cgaggcggtg ctcgtgccca
      841 cggaagcggt catcgtcacg ggcgagcgca gcgtggtcgt cgtggatcgc ggcgaaggcc
      901 gcttcgagcc ggtcgacgcg gtcatcggcc gcgaagagca cgggcgcacc gagatcctca
      961 agggcgtcga ggcgggggcg cgcgtcgtcg tctcgggaca attcctcatc gattccgaag
     1021 cgagcctgcg cggcgccggt cggcgcatgt cgcccgaagc gccgatcgct cccgccgggg
     1081 agaagaaatg atcgcgcggc tcattcgctg gtcgatcgcg aaccgcttcc tcgtgctcct
     1141 cgcgtccgtg ggcgtcgcgg cctggggcgc gtggtcgatg gcgcgcacgc cgctcgacgc
     1201 cctgccggac ctctccgacg tgcaggtgat catccgcacg ccctatcccg gacaggcgcc
     1261 gcgcatcgtc gaggaccagg tcacctatcc gctcacgacg acgatgatgt cggttccggg
     1321 cgccaaggcc gtgcgcgggt actcgttctt cggggactcg ttcgtctacg tgctcttcga
     1381 ggacggaacc gatctctatt gggcgcgctc gcgcgtgctc gagtacctca accaggtgca
     1441 gtcgcgcctt cccgcgcagg cgaaggcttc cctcgggccc gatgcgacgg gcgtgggctg
     1501 ggtctacgag tacgcgctgg tggaccgcac gggccgccac gatctcgggg agctgcgcgc
     1561 gatccaggac tggttcctca agtacgagct gaagagcgtg cccgacgtgg ccgagatcgc
     1621 caccgtcggc ggcatggtgc gcgagtacca ggtcgtcctc gatcccgcga ggctgcgggc
     1681 ctatggaatc tcccatgggc gcgtggtcgc ggcgatcgaa ggggcgaatc gcgaagcggg
     1741 cggctcggtc ctcgagatgg gagaagccga gtacatggtg cgctcgcgcg gctacctgaa
     1801 gaccctggaa gacttccgct cgatccccct gatggtgggc gaatcgtcca cgcccgtgac
     1861 gctgggagac gtgg
//
LOCUS       DASVII010000181         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_80435, whole
            genome shotgun sequence.
ACCESSION   DASVII010000181 DASVII010000000
VERSION     DASVII010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80435"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..>1656
                     /locus_tag="VF309_02330"
     CDS             10..>1656
                     /locus_tag="VF309_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX7465448.1"
                     /translation="MNATLAGTRAAHRLDVDATMPRDTRVRASFQGGLDAKSPALSWN
                     GRVESLAMTGRGAFALVAPATLSLGASKAELGDATLRGEWGEAHLAVTRWTPRALDVK
                     GATSGLQIQNLARSFRLGTVPRSDLVIAGHWDIHSAETFEGTIDVKRVSGDLRLGEPP
                     LPLGLTDLTVHAEASQGRARATVNATGERVGRLSGEGSGLIVRGDSGWEFARSSPVEG
                     KVVASIPDLEALAAWLGPDAKLGGRLDANVAISGTGADPRFDGQARAASLVVREPQSG
                     FEIAEGEVALRLEGRKLSIERFVAKTPWRASPEALERIRRGEIPPAGGNITAEGSIDL
                     AARHGAIVIKADKVPVTQLPKRFVAVSGEAKLEAGAKGLDATGAFKVDAGWIGALAEA
                     LPSPSEDVVVIRASQPPALDEPAKKEPMRLDLKIALNDRLSFQGRGLDTRLTGDVRIT
                     GEVGSTLRATGVIRTTGGTYEGYGQKLVIERGVLSFSGPLDNPQLNVLALRKGLPVEA
                     GVEIVGTTTRPRVRLVSSPDVPEPEKLSWLVLGRGASDSAP"
BASE COUNT          232 a          591 c          607 g          226 t
ORIGIN      
        1 gcggacacct tgaacgcgac gctcgcggga acgcgcgccg cgcaccgcct cgatgtggac
       61 gcgacgatgc cgcgcgatac acgcgttcgc gcatccttcc agggcggcct cgatgcgaag
      121 agcccggcgc tctcgtggaa cgggcgcgtg gaatcgctcg ccatgacggg gcgcggggcc
      181 ttcgcgctcg tcgcgcctgc aacgttgtcc ctcggcgctt cgaaggccga gctcggcgac
      241 gcgacgttgc gcggcgagtg gggcgaggcg cacctcgccg tcacgcgctg gacgccgcgc
      301 gcgctcgacg tgaagggtgc gacgtcggga ttgcagatcc agaacctcgc gcgcagcttc
      361 cggctcggca ccgtgccgcg ctccgacttg gtgatcgcgg gccattggga catccactcc
      421 gccgaaacct tcgaaggcac gatcgacgtg aagcgcgtgt cgggcgacct gcgcctgggc
      481 gagccgccgc tgccgctcgg cctcacggat ctcacggtgc atgccgaggc ctcccagggc
      541 cgggcgcgcg ccacggtgaa tgcgaccggc gagcgcgttg gccgcctctc gggggaggga
      601 agcgggctca tcgtgcgtgg cgacagcggc tgggagttcg cgcgttcctc gcccgtggag
      661 ggcaaggtcg tcgcgtccat cccggacctc gaagcgctcg ccgcgtggct cgggcccgac
      721 gccaagcttg gcgggcgcct cgatgcgaat gtcgcgatct ccggcaccgg cgccgatccg
      781 cgcttcgacg gccaggcacg cgccgcgagc ctcgtggtgc gcgagccgca gagcggcttc
      841 gagatcgcgg aaggcgaggt ggcgctgcgc ctcgaaggac gcaagctctc catcgagcgc
      901 ttcgtcgcga agacgccgtg gcgagcctcg cccgaggcgc tggagcgcat ccggcgcggc
      961 gagatcccgc ccgcgggcgg caacatcacc gccgagggct cgatcgacct ggccgcgcgc
     1021 cacggcgcga tcgtgatcaa ggcggacaag gtgccggtca cgcagcttcc caagcgcttc
     1081 gtcgcggtct ccggcgaagc gaagctcgag gcgggcgcga aggggctcga cgcgaccggg
     1141 gccttcaagg tcgacgcggg atggatcggc gcgctcgccg aggcgctgcc ttcgccgtcc
     1201 gaggacgtcg tcgtgatacg cgcgtcccag cccccggcgc tggacgagcc cgcgaagaag
     1261 gagccgatgc gcctcgacct gaagatcgcc ctgaacgatc gcctgtcgtt ccagggccgc
     1321 gggctcgaca cgcgccttac cggcgacgta cgcatcaccg gcgaggtcgg cagtacgctg
     1381 cgcgctaccg gcgtgatccg cacgaccggc gggacctacg agggctacgg ccagaagctc
     1441 gtgatcgagc gcggcgtcct cagcttcagc ggcccgctcg acaacccgca gctgaacgtc
     1501 ctcgcgttgc gcaagggctt gccggtggag gcgggcgtcg agatcgtcgg cacgacgact
     1561 cgcccgcgcg tgcgcctcgt gtcgtcgcct gacgtgcccg agcccgagaa gctctcgtgg
     1621 ctcgtgctgg gacgcggcgc ctcggactcc gctccc
//
LOCUS       DASVII010000182         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_224457,
            whole genome shotgun sequence.
ACCESSION   DASVII010000182 DASVII010000000
VERSION     DASVII010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224457"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..681
                     /gene="coaE"
                     /locus_tag="VF309_02335"
     CDS             55..681
                     /gene="coaE"
                     /locus_tag="VF309_02335"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228308.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7465449.1"
                     /translation="MGRPLSGVIVIGLTGGIGSGKTTVAGLLAELGASVVDTDAIAHS
                     LTGAGGGAIEGIRKAFGAGFIGADGALDRPAMRDLAFEDPAARARLESILHPAIRAAA
                     DRALAEASGAYAVIVVPLLFETRGYLDRVARTLAVDCAEHLQAERTARRSGLAAGQVR
                     RIMDAQWPRWRRLQMADDTVWNGAGEDALDPQCERLHRLYAALGAMAA"
     gene            736..1491
                     /gene="zapD"
                     /locus_tag="VF309_02340"
     CDS             736..1491
                     /gene="zapD"
                     /locus_tag="VF309_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX7465450.1"
                     /translation="MITYEYPLNERVRTLLRLEDLYDRVRYFLSESDPNGHHAALTSM
                     FEILEVASRADLKSDLLQELDRQRTFLEALRSNPAISEDKLNGVLKEIDSAFANLHAT
                     SGKTGQSLRENEWLMAIKQRVGIPGGTSEFDLPSYHHWLHHPVERRRADLEEWLKPLL
                     PIHAALSIVLRVLRESGRTVSLIAFQGVYQQTPAEKPAQMLRLALEDGLACVPEISAN
                     KYALNVRFLLPEGVQRTRVYEHDVPFELAFCNL"
     gene            1488..1709
                     /locus_tag="VF309_02345"
     CDS             1488..1709
                     /locus_tag="VF309_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX7465451.1"
                     /translation="MTDSPLSGRILACPACGKATVFAPSNRWRPFCSERCRMTDLGAW
                     ATESYRVPAKAAEDEGDATPDASSSDTEG"
     gene            1778..2245
                     /locus_tag="VF309_02350"
     CDS             1778..2245
                     /locus_tag="VF309_02350"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX7465452.1"
                     /translation="MFLEAESPQYIHHSVPNRSLGPAGRRIFLALMAVTTLGIGMLAV
                     VVGAWPVMPFAGAEVALVFFAFHLVRLHDADFERLEIGPHEVRVESRDAESLTRFVAH
                     RPWARVIVRERGARCTLGLAYAGRTVQLGRLMSDEGRRKLAEELRGRIAVTAK"
     gene            2271..3455
                     /gene="coxB"
                     /locus_tag="VF309_02355"
     CDS             2271..3455
                     /gene="coxB"
                     /locus_tag="VF309_02355"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7465453.1"
                     /translation="MLLKHLRALAVLAATFIASLAAHADYHVDILPPATPVSREIYDL
                     HFGILWVCVAIFVVVFGAMFWSVFKHRRSAGAKASQFHENTTIEVIWTIVPFIILIGM
                     AYPATRTVLNMKDTSNADMSIKVTAYQWKWEYDYQQDGVKFLSNLATPRDQIEERDGK
                     VGAPKNENYLLEVDNPMVVPVGKRVRLLITSNDVIHGWYVPQLGINQYGIPGFIKDAW
                     FEIDKAGTYRGQCSQICGKEHGYMPIVVVAKSPEEYAAWVKDAKAKMPPPATVAAATP
                     EAPAGATPPAADPNKKWTLDELKSAGEKLYAANCAACHQPTGKGMPPAFPALDGSKVA
                     TGPKAAHLATVLKGRPGTAMAPFAQLADADLAAIITYERNAWGNHTGEAIQPAEIAAA
                     RK"
BASE COUNT          625 a         1260 c         1191 g          549 t
ORIGIN      
        1 cctcgcgctg ggaggcatcg ccgcgctcct ctggggacgc gaagccgtca ccgcgtgggt
       61 cggccgcttt ccggcgtgat cgtcatcggg ctcacgggcg gaatcggctc gggcaagacc
      121 accgttgccg gcctcctcgc ggagctgggg gcatcggtcg tcgacaccga cgcgatcgcg
      181 cactccctca ctggcgcggg gggcggggcg atcgagggga ttcgcaaggc attcggcgcc
      241 gggttcatcg gggccgacgg cgcgctcgat cgccccgcga tgcgcgatct cgcgttcgag
      301 gatccggccg cccgcgccag gctcgaatcg atcctgcacc cggcgatccg cgcggccgcc
      361 gatcgcgccc tcgccgaggc atccggcgcc tacgcggtga tcgtggtgcc cctcctcttc
      421 gagacccgcg gctacctcga tcgcgtggcg cgcacgctgg cggtcgattg cgccgagcac
      481 ctgcaggccg agcgcacagc caggcgttcg ggactcgcgg ccgggcaggt ccgccggatc
      541 atggacgcgc agtggccgcg ctggagacgc ctgcagatgg ccgacgacac ggtctggaac
      601 ggcgccgggg aggacgcgct ggatccgcaa tgcgagagat tgcaccgtct ctacgcggcg
      661 cttggcgcga tggcggccta gatttgtcaa ggcgatgaca ttgccccaca atccggccca
      721 tccgggggcg ggtccgtgat cacctacgaa tatcctctca acgagcgcgt caggaccctc
      781 ctcaggctcg aggaccttta cgatcgcgtg cgctatttcc tctccgagag cgatcccaac
      841 gggcatcacg cggccctcac cagcatgttc gagatcctcg aggtcgcgag ccgcgccgac
      901 ctgaagagcg acctgctcca ggagctcgac cgccagcgca ccttcctcga ggcgctgcgc
      961 agcaacccgg cgatctccga ggacaagctg aacggcgtcc tgaaggagat cgacagcgcc
     1021 ttcgcgaacc tccacgcgac ctcgggcaag accggtcaga gcctgcggga gaacgagtgg
     1081 ctgatggcga tcaagcagag ggtgggaatt ccgggcggca cgagcgaatt cgacctgccc
     1141 tcgtaccatc actggctcca ccatccggtc gagcgccgcc gcgccgacct cgaggaatgg
     1201 ctcaagcccc tcctgccgat ccatgcggcg ctctcgatcg tgctgcgcgt gctgcgcgag
     1261 agcggccgca cggtgtccct catcgcgttc cagggcgtgt accagcagac gcccgcggaa
     1321 aagcccgcgc agatgctgcg cctcgcgctg gaggacgggc ttgcctgcgt ccccgagatc
     1381 agcgcgaaca agtacgccct caacgtgcgc ttcctcctcc cggagggcgt gcagcgcacg
     1441 cgcgtgtacg agcacgacgt ccccttcgag ctcgctttct gcaacctgtg accgattcgc
     1501 cgctttccgg ccggatcctg gcctgccccg catgcggcaa ggcgacggta ttcgccccct
     1561 cgaaccgctg gcgcccgttc tgcagcgagc gctgccggat gaccgacctc ggcgcatggg
     1621 cgacggagtc ctatcgcgtg cccgcgaagg ccgcggaaga cgaaggggac gcaaccccgg
     1681 acgcgtcatc cagcgatacg gaagggtgaa aggcgcacgc gcttttgcgc gccgagcgcc
     1741 gctcggctaa aatagccccg cttggagcgg ctccccgatg ttccttgaag cggaatcccc
     1801 gcagtacatt caccattcgg tgcccaaccg ttcgctgggc ccggctggca ggcgcatctt
     1861 tctcgcgctg atggcggtga ccacgctggg gatcgggatg ctcgccgtcg ttgtcggggc
     1921 atggcccgtg atgccgttcg cgggggccga ggtcgccctg gtcttttttg cgtttcacct
     1981 cgtgcgactg cacgacgccg atttcgagcg cctcgagatc gggcctcacg aggtgcgtgt
     2041 ggagtcgagg gacgccgaat cgctcacgcg cttcgtggcc caccggccct gggcgcgcgt
     2101 catcgtgagg gagcgaggcg cgcggtgcac cctcggcctc gcatatgcgg ggcgtacggt
     2161 gcagctgggg aggctcatgt cggacgaggg gcggcgcaag ctcgcggaag agctccgcgg
     2221 gcggatcgca gtcaccgcga aatagaaact cgtaaaaaaa aggtctggcc atgcttttga
     2281 aacatctgcg cgccctcgcc gttctcgcgg cgaccttcat cgcttcgctc gcggcgcacg
     2341 ccgattacca cgtcgacatc cttccgcccg ccactccggt ctcgcgagag atctacgacc
     2401 tccacttcgg gatcctctgg gtgtgcgtgg ccatcttcgt ggtcgtgttc ggcgcgatgt
     2461 tctggtcggt gttcaagcac cgccgcagcg ccggcgccaa agcctcgcag ttccacgaga
     2521 acacgacgat cgaggtcatc tggacgatcg tccccttcat catcctcatc ggcatggcct
     2581 acccggcgac gaggaccgtc ctcaacatga aggacacgtc caacgccgac atgtcgatca
     2641 aggtgacggc ctaccagtgg aaatgggaat acgactacca gcaggacggc gtgaagttcc
     2701 tttcgaacct cgccacgccg cgcgaccaga tcgaggagcg cgacggcaag gtgggcgcgc
     2761 cgaagaacga gaattacctc ctcgaggtcg acaatccgat ggtggtgccg gtgggcaaga
     2821 gggtgcgcct tctcatcact tcgaacgacg tgatccacgg ctggtacgtg ccgcagctcg
     2881 gcatcaacca gtacggcatc ccggggttca tcaaggacgc gtggttcgag atcgacaagg
     2941 cgggcacgta ccgcggccag tgcagccaga tctgcggcaa ggagcacggc tacatgccga
     3001 tcgtcgtcgt cgccaagtcg cccgaggaat acgcggcctg ggtgaaggac gccaaggcga
     3061 agatgccgcc accggcgacg gtcgcggccg cgacgccgga agcgcccgcc ggcgccacgc
     3121 ccccagccgc cgaccccaac aagaagtgga cgctcgacga gctgaagtcg gccggcgaga
     3181 agctctatgc ggcgaattgc gcggcgtgcc accagccgac cggcaagggc atgcccccgg
     3241 cgttcccggc cctcgacggc tcgaaagtcg cgaccggacc gaaggctgcg catctcgcga
     3301 cggtcctcaa gggccggccg ggaaccgcga tggccccgtt cgcgcagctt gccgacgccg
     3361 acctcgccgc aatcatcacg tacgagcgca acgcctgggg caaccacacg ggcgaagcga
     3421 tccagccggc cgagatcgcg gccgcgcgca agtagcgaat cccagaaagc ggagcaaaca
     3481 tgagcacagc gagccacacc cacgttcccg gggctctcga cgaccacggt cacgccgagg
     3541 cccatgacca ccacggcccg cccgcgggct tcacccgctg gatgtacacg acgaaccaca
     3601 aggacatcgg cacgatgtac ctctg
//
LOCUS       DASVII010000183         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_619895,
            whole genome shotgun sequence.
ACCESSION   DASVII010000183 DASVII010000000
VERSION     DASVII010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619895"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..>1789
                     /gene="rpoB"
                     /locus_tag="VF309_02360"
     CDS             312..>1789
                     /gene="rpoB"
                     /locus_tag="VF309_02360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881213.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7465454.1"
                     /translation="MTYTFTEKKRIRKSFAKRAAVLDVPFLLATQINSYAEFLQSEIP
                     GDTRREMGLQSAFTSVFPISSHSGNARLEFVSYSLGEPPFDVVECQQRGLTFASPLRA
                     KVRLTIMDREASKPTVKEVKEQEVYMGEIPLMTRNGSFIINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDYLYFRVDRRRKMPVTILLKALG
                     YNPEQILTHFFSFDTFHITKKGIEFEVVPERLKGEIAKFDIVTKAGKVIVQKDKRITV
                     KHIREMEAAGMKKLTVEPDFLVNRTLAKNLVNKETGEVVANVNEEITEAALKKILDAG
                     VEEVKTLYTNDLDHGPYISQTLRVDESTDALNAQVAIYRMMRPGEPPTEDAVKTLFAG
                     LFFNEDRYDLSDVGRMKFNRRVGRDELTGPMTLSNEDIVAVIKILVELRNGRGEIDDI
                     DHLGNRRVRSVGELAENQFRSGLVRVERAVRERLSQAESENL"
BASE COUNT          350 a          594 c          557 g          288 t
ORIGIN      
        1 gaagaaaatg gaagaggcgg gtgcgaaggt cgaggtcaag taaatccgga cctcgcggta
       61 gaaatgcagc ggggcggtcg ttggagcgga cagttcctgc cgctcaacga cgcgtagcag
      121 agtgcaaagc caagcctctc cgggggcttg tctgtgtcct ctgaagcgac tgcggaggac
      181 gggtttggcc gggccatgcg ttccagcatg gctgtccgcc agcggctcgt agtggcgagc
      241 caccaggcca tcagtgccag tcgggtgcaa tgcggcccat ggagacccat ggccgtgatt
      301 tcggagctcc catgacctac acgttcaccg agaagaagcg catccgcaag agcttcgcga
      361 agcgcgcggc cgtcctcgac gtgcctttcc tcctcgccac gcagatcaac tcgtacgccg
      421 agttcctgca gtccgagatc cccggcgaca cgcgccgcga gatgggcctg cagtcggcgt
      481 tcacctccgt gttcccgatc tcgagccact cgggcaacgc ccgtctcgag ttcgtgagct
      541 actcgctcgg cgagccgccg ttcgacgtgg tcgagtgcca gcagcgcggc ctcacctttg
      601 cgagcccgct gcgcgccaag gtgcgcctca ccatcatgga ccgcgaggcc tccaagccca
      661 cggtgaagga ggtgaaggag caggaggtgt acatgggcga gatcccgctc atgacccgca
      721 acggctcctt catcatcaac ggcaccgagc gcgtgatcgt ctcgcagctg catcgctcgc
      781 ccggagtgtt cttcgagcac gaccgtggca agacgcactc ctcgggcaag ctcctgttct
      841 cggcgcgcgt gattccctat cgcggctcgt ggctcgactt cgagttcgac ccgaaggact
      901 acctctattt ccgcgtcgac cgccgccgca agatgccggt cacgatcctg ctgaaggcgc
      961 tcggctacaa ccccgagcag atcctcacgc atttcttctc gttcgacacg ttccacatca
     1021 ccaagaaggg catcgagttc gaggtggtgc ccgagcgcct gaagggcgag atcgccaagt
     1081 tcgacatcgt caccaaggcg ggcaaggtca tcgtccagaa ggacaagcgc atcaccgtga
     1141 agcatatccg cgagatggaa gccgcgggca tgaagaagct cacggtcgag ccggatttcc
     1201 tcgtgaaccg caccctcgcg aagaacctgg tcaacaagga gaccggtgaa gtcgtcgcga
     1261 acgtgaacga ggagatcacc gaggccgcgc tgaagaagat cctcgacgcc ggcgtcgagg
     1321 aagtgaagac cctctacacg aacgacctcg accacggccc gtacatctcg cagacgctgc
     1381 gcgtcgacga gtcgaccgac gcgctgaacg cgcaggtcgc gatctaccgc atgatgcgcc
     1441 ccggcgagcc gcccacggaa gacgcggtga agaccctctt cgcgggcctc ttcttcaacg
     1501 aggatcgcta cgacctttcc gacgtcggcc gcatgaagtt caaccgccgc gtcgggcgcg
     1561 acgagctcac cggcccgatg accctctcca acgaggacat cgtcgcggtg atcaagatcc
     1621 tggtcgagct gcgcaacggc cgcggcgaga tcgacgacat cgaccacctc ggcaaccgcc
     1681 gcgtgcgttc cgtgggcgag ctcgccgaga accagttccg ctcgggcctg gtgcgcgtcg
     1741 agcgcgcggt gcgcgagcgc ctctcgcagg ccgagagcga aaacctcat
//
LOCUS       DASVII010000184         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_116787,
            whole genome shotgun sequence.
ACCESSION   DASVII010000184 DASVII010000000
VERSION     DASVII010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116787"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..522
                     /gene="ybeY"
                     /locus_tag="VF309_02365"
     CDS             55..522
                     /gene="ybeY"
                     /locus_tag="VF309_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7465455.1"
                     /translation="MSGRPLVSSRRRPGPNLELLIQRASRAAHIPADRALRKWVRAAF
                     ARPATVTVRYVGEAEGRRLNREFRGKDHATNVLSFAYEAKPLAGDIVICAPVVAREAR
                     AQGKALDAHHAHLVIHGALHLQGYDHERSAREAAAMEKRERTLLAKLGFPDPY"
     gene            553..1386
                     /locus_tag="VF309_02370"
     CDS             553..1386
                     /locus_tag="VF309_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX7465456.1"
                     /translation="MNDPPKPSLLERLSTMLLREPEDRDQLVGLLHSAFERRLLDAEA
                     LSMIEGVLQVSETQVRDVMIPRSQMDMIDVADSPDKFIPYVIETSHSRFPVFEDNRDN
                     VIGILLAKDLLRFYAEEEFNVREMLRPAVFVPEAKRLNVLLKEFRANRNHIAIVVDEY
                     GGVSGLITIEDVIEQIVGDIEDEYDFDETEDNILVDRSGRYRVKAITEIADFNSHFDS
                     KFSDEDYDTVGGLVVGRFGRLPKRGESIAIDGFNFQVLRADSRKVHSLLVERPKAKEP
                     K"
     gene            1383..>1569
                     /locus_tag="VF309_02375"
     CDS             1383..>1569
                     /locus_tag="VF309_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465457.1"
                     /translation="MTRESPPAPPAVPAARVLARGVLLPAVAGGACVLGFAPFGAWPV
                     PIVALAALFAVWVRSTSP"
BASE COUNT          258 a          546 c          521 g          244 t
ORIGIN      
        1 gctggtgcag gccatcgtcg acgcgtacga ccgccgggtc agagtgaaga agtcgtgagc
       61 ggcaggccgc tcgtgtcgtc ccggcgaagg ccgggaccca atctcgagct tctcatccaa
      121 cgcgcctcgc gcgccgccca cattcccgct gaccgtgcgc tgcgcaaatg ggtccgcgcc
      181 gcgttcgcgc ggccggcgac cgtcacggtg cgctacgtcg gcgaggccga gggccgccgc
      241 ctcaatcgcg agttccgcgg caaggaccat gcgaccaacg tgctgagctt cgcctacgaa
      301 gcgaaaccgc tcgcgggcga catcgtgatc tgcgcgccgg tggtcgcgcg ggaggcgaga
      361 gcacagggca aggcgctcga tgcgcaccac gctcacctcg tgatccacgg ggcgctccac
      421 ctgcagggat acgaccacga gcgaagcgcg cgcgaggccg ccgcgatgga aaagcgcgag
      481 cgtacgctcc tcgcaaagct ggggtttccc gatccgtatt agagtatcgg cgtctccccc
      541 cgctttcccc gcatgaacga cccgcccaag ccgagcctgc tcgagcgcct ttccaccatg
      601 ttgctgcgcg agcccgagga ccgggaccag ctggtggggc tgctgcattc ggcgttcgag
      661 cgccgcctcc tcgacgctga ggcgctctcg atgatcgagg gggtgctgca ggtttccgag
      721 acccaggtcc gcgacgtcat gatcccgcga tcgcagatgg acatgatcga cgtcgccgac
      781 agccccgaca agttcatccc gtacgtgatc gagacgtcgc actcgcgctt tccggtcttc
      841 gaggacaacc gcgacaacgt gatcggcatc ctcctcgcca aggacctgct gcgcttctac
      901 gccgaggaag agttcaacgt gcgcgagatg ctgcggcccg cggtcttcgt ccccgaggcg
      961 aagcgcctca acgtgctcct caaggaattc cgcgccaacc gcaaccacat cgcgatcgtc
     1021 gtcgacgagt acggcggggt gtcggggctc atcaccatcg aggacgtgat cgagcagatc
     1081 gtgggcgaca tcgaggacga gtacgacttc gacgagaccg aggacaacat cctcgtcgac
     1141 aggagcgggc gctaccgcgt gaaggcgatc accgagatcg ccgatttcaa cagccacttc
     1201 gactcgaagt tctccgacga ggactacgac accgtgggcg gcctcgtcgt cgggcgcttc
     1261 gggcggctgc ccaagcgggg cgaatcgatc gcgatcgacg ggttcaactt ccaggtcctg
     1321 cgcgccgaca gccgcaaggt gcactccctc ctcgtcgagc gcccgaaggc gaaggagccg
     1381 aagtgacccg cgagtcgccg ccggcgccgc ccgcggtgcc ggcggcgcga gtgcttgcgc
     1441 gcggcgtgct gctacccgcg gtggcgggcg gcgcctgcgt tctcggtttc gcgcccttcg
     1501 gcgcatggcc cgtgccgatc gtcgcgctgg cggcgctctt cgcagtctgg gtgcggagta
     1561 cctcgcccc
//
LOCUS       DASVII010000185         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_224525,
            whole genome shotgun sequence.
ACCESSION   DASVII010000185 DASVII010000000
VERSION     DASVII010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224525"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /gene="ggt"
                     /locus_tag="VF309_02380"
     CDS             complement(<1..1610)
                     /gene="ggt"
                     /locus_tag="VF309_02380"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7465458.1"
                     /translation="MLASLLCAVTFAVAQERPSQPEPPSGWNAKKLAHAKKYMVAAAN
                     PLAVDAGVKMLERGGSAIDAMIATQLVLNLVEPSSSGLGGGAFLLFYDAKAKAIRAID
                     ARETAPAGATPALFTKPDGTQMAFLDARVGGRSVGTPGTPRLLEVAHARYGKLPWATL
                     FEPAIELAEKGFPVSPRFNRLIGEDAGLANEPAARAYFFDDAGKPRAVGAIVRNPAFG
                     ATLRAIAAKGVDAYYTGDIAKDIVAAVHAHRNAGTLSLEDLAAYRVREVESLCGTYRT
                     WKLCGMPPSSSGGIAVLQILGELEPRDMSKVRPNSVEAVHVVSEAERLAFADRNRYVA
                     DDRFVDVPVKGLVDRGYTASRGQLIRPEVSMGKAQAGTPPGVKVALADDPVDEVMGTT
                     HISVVDGDGNAVSMTTTIESFFGSRIMVRGFLLNNELTDFNFLPVEEGAQVANSVAPG
                     KRPRSSMAPFLVFDRAGALDMTIGSPGGSLIIGYMSKTLVGILDWGLDVQSAIDLPNF
                     GSRNGPTEIEKGSALEGTQAALKAMGHDV"
BASE COUNT          288 a          608 c          618 g          319 t
ORIGIN      
        1 acgtcgtggc ccatggcttt caacgcggcc tgggtgccct cgagcgcgct gcctttctcg
       61 atctccgtgg gcccgttgcg gctgccgaag ttgggaaggt cgatcgcgga ctgcacgtcg
      121 agcccccaat cgaggatgcc cacgagcgtc ttcgacatgt agccgatgat gaggctgccg
      181 ccagggctgc cgatggtcat gtcgagcgcg ccggcgcgat cgaagacgag gaagggcgcc
      241 atcgagctcc tcgggcgctt gcccggggcg acgctgttgg cgacctgcgc tccctcctcc
      301 accgggagga aattgaagtc ggtgagctcg ttgttgagca ggaacccgcg aaccatgatg
      361 cggctgccga agaaggattc gatggtcgtg gtcatcgaca ccgcgttgcc gtcgccgtcg
      421 acgacggaga tgtgcgtcgt gcccatcacc tcgtcgacgg gatcgtcggc gagcgcgacc
      481 ttcacgcccg gcggcgtccc cgcctgcgcc ttgcccatcg acacttcggg acggatgagc
      541 tgcccgcgac tcgcggtgta gccgcgatcg acgaggccct tcacgggaac gtcgacgaag
      601 cgatcgtcgg cgacgtagcg attgcggtcg gcgaatgcga ggcgctcggc ttccgagacg
      661 acgtgcaccg cctcgacgga gttgggccgc accttcgaca tgtcgcgcgg ctcgagctcg
      721 cccaggatct gcagcaccgc gatgccaccc gacgacgagg gcggcatgcc gcagagcttc
      781 cacgtccgat acgttccgca cagcgattcc acttcgcgaa cgcgatacgc ggcgagatcc
      841 tcgagcgaga gcgtgccggc gttgcgatgc gcatgcaccg cggcgacgat gtccttcgcg
      901 atgtcgccgg tgtagtacgc gtcgacgccc ttcgccgcga tcgcgcgcaa cgtcgcgccg
      961 aacgcgggat tcctgacgat cgccccgacc gcccggggct tgccggcatc gtcgaagaag
     1021 taagcgcgcg cggcgggctc gttggcgagc cccgcgtcct ctccgatcag gcgattgaag
     1081 cgcggcgaga ccgggaagcc cttttcggcg agctcgatcg cgggctcgaa tagcgtggcc
     1141 catggaagct ttccatagcg cgcgtgcgcg acttcgagga ggcgcggcgt cccgggcgtg
     1201 ccgaccgagc ggccgccgac gcgcgcatcg aggaacgcca tctgcgtgcc gtcgggcttc
     1261 gtgaagaggg cgggtgtcgc gcccgcgggc gcggtctcgc gcgcgtcgat cgcgcggatc
     1321 gccttcgcct tcgcgtcgta gaagagcagg aacgcgccgc cgcccaggcc cgaggagctc
     1381 ggctcgacga ggttcaacac gagctgcgtg gcgatcatcg cgtcgatggc gctgccgccg
     1441 cgctcgagca tcttcacccc cgcgtccacc gcgagcgggt tcgccgccgc caccatgtac
     1501 ttcttcgcgt gcgcgagctt cttcgcgttc cagccgctgg gcggctccgg ctgcgacggc
     1561 ctttcctgcg ctaccgcgaa cgtgactgcg cagaggagtg atgcgagcaa aattctttga
     1621 atccgcagat aaacgcagat aaacgctgta ccccgactgc aagtatccgg gagtctcgtg
     1681 tctgcgtttg gcaatggggc ctggtcgctc gagaatctac tcgccaagct ctgagccaag
     1741 gggtctaccg cgttcatctg cgtttatctg cggatagtcg ctttactgaa tcccgccgat
     1801 ggcgatgtac ttgatctcga ggaagtcctc gat
//
LOCUS       DASVII010000186         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_332625,
            whole genome shotgun sequence.
ACCESSION   DASVII010000186 DASVII010000000
VERSION     DASVII010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332625"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VF309_02385"
     CDS             complement(<1..799)
                     /locus_tag="VF309_02385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7465459.1"
                     /translation="MAELGEGGMGEVYRARDTRLGREVAIKILRADRIDDRRRFEREA
                     RTIAGLDHPNLLTIFDVGTEGGTDYLVTELLRGETLRARLDRTGRLSWIERARIAEAI
                     ARGLAGAHARGIVHRDIKPENVFLTDDSRVKILDFGLAHVATPIDAATMNQTAPGALL
                     GTVPYMAPEQACGDPVDARTDLFGLGAVTYECLTGVSPFRRDTIMATFAAVIGDTPRS
                     LTTDGVRDDLASIVARCLAKLPNERPESASMVADLLARALASQSGSGS"
     gene            complement(849..1307)
                     /locus_tag="VF309_02390"
     CDS             complement(849..1307)
                     /locus_tag="VF309_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465460.1"
                     /translation="MNDEVLVRGRPAVILLAEDNDNDVELTRLGFRRAKFAVELHHVS
                     NGEDCLTFLRKQGPYADVPTPDIILLDLNMPRMDGIEVMQEINKDEQLKHLVTVVMTS
                     SKADEDVMRSYKLRCSSYLVKPINFDAFAKMIESLGDYWFTLVMLPPTGK"
     gene            complement(1304..>1929)
                     /locus_tag="VF309_02395"
     CDS             complement(1304..>1929)
                     /locus_tag="VF309_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7465461.1"
                     /translation="RSIASFVDLLRSTYAETLDAQAKDWLSRTTQSVDHLQTLIRDLL
                     EYSRVDVEPRPFEKVSMREVFERASSLLDAAVRDAAATITCDELPEVTGDRSQLVQLL
                     LNLLGNAIKYRGTEAPRVHVTGQTRADDWLFEVRDNGIGIAEKHHQQVFEVFKRLHDQ
                     KQYPGTGIGLAICRRVLDRHGGKIWVESKLGEGSAFFFTIAKGETQR"
BASE COUNT          310 a          629 c          640 g          350 t
ORIGIN      
        1 cgctgccgga cccgctctgc gatgcgagcg cgcgcgccag caaatccgca accatcgacg
       61 cggactccgg ccgttcattc gggagcttcg cgaggcaacg cgcgacgatg ctcgcgaggt
      121 cgtcgcgcac accgtcggtg gtgagcgagc gaggcgtgtc gccgatcacc gcggcgaatg
      181 tcgccatgat ggtgtcgcgg cgaaatggcg agacgccggt caggcactcg tacgtgaccg
      241 cgccgagccc gaacagatcg gtgcgcgcgt cgacgggatc gccgcacgcc tgctccgggg
      301 ccatgtacgg cacggtgccg agtagcgcgc cgggcgcggt ctggttcatg gtcgcggcgt
      361 cgatcggggt agccacgtgg gcgaggccga agtcgaggat cttcacgcgg ctgtcgtccg
      421 tgaggaacac gttctcgggc ttgatgtcgc ggtgcacgat gccgcgcgcg tgcgcgcccg
      481 cgagtccccg cgcgatcgcc tcggcgattc gcgccctctc gatccacgac aggcggccgg
      541 tccggtcgag ccgcgcgcgc agcgtctcac cgcgcagcag ctcggtcacg aggtagtcgg
      601 tgccgccctc ggtgccgacg tcgaagatcg tcaggaggtt cggatgatcg agcccggcga
      661 tcgtgcgcgc ctcgcgctcg aagcgacgcc ggtcatctat ccgatccgcg cgcaggatct
      721 tgatcgcgac ttcgcggccg agccgggtgt cacgcgcacg gtagacctcg cccatgccgc
      781 cctcaccgag ctctgccaca acctcatacg gtccgacgtg atcgccctgt gagaagcgca
      841 tccgcgtgtt atttcccggt cggcggcagc atgacgagcg tgaaccagta gtcgccgagt
      901 gactcgatca tcttcgcgaa cgcatcgaag ttgatcggct tgacgaggta cgagctgcag
      961 cgaagcttgt acgagcgcat gacgtcttcg tcggccttcg acgacgtcat cacgacggtc
     1021 acgaggtgct tgagctgctc gtccttgttg atctcctgca tcacctcgat gccgtccatc
     1081 cgcggcatgt tgagatcgag caggatgatg tcgggcgtcg ggacgtcggc gtacgggcct
     1141 tgcttgcgca agaaggtgag gcagtcctcg ccgttggaca cgtgatggag ctcgaccgca
     1201 aacttggctc gccgaaatcc aagtctggtc agctcgacgt cgttgtcgtt gtcctctgcg
     1261 aggaggatca ccgcgggtcg tccccggacc agtacttcgt cgttcatcgc tgagtctcgc
     1321 cttttgcgat cgtgaaaaag aacgcgcttc cctcgcccaa cttggattcc acccagatct
     1381 taccaccgtg gcgatcgagg acgcggcggc agatcgccag cccgatgcca gtgccgggat
     1441 actgcttctg atcgtgcagg cgcttgaaca cttcgaacac ctgctggtgg tgcttctccg
     1501 cgatcccgat gccgttgtca cgaacttcga acagccagtc gtcggcgcga gtctgccccg
     1561 tgacgtggac gcgcggcgct tccgtgccgc ggtacttgat cgcgttgccc aagaggttca
     1621 ggagcagttg cacgagctgc gagcgatcgc cggtgacctc gggcagctcg tcgcacgtga
     1681 tggtcgccgc cgcgtcgcgc accgcagcgt cgagcagcga gctcgcgcgc tcgaacacct
     1741 cgcgcatcga gaccttctcg aacggtcgcg gctcgacatc gacgcgcgag tactctagta
     1801 gatcgcggat cagcgtctgc agatggtcga ccgactgggt cgtgcgcgat agccagtcct
     1861 tggcctgggc atcgagcgtc tcggcatagg tcgaccgcag gaggtcgacg aagctcgcga
     1921 tgctgcgca
//
LOCUS       DASVII010000187         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_404392,
            whole genome shotgun sequence.
ACCESSION   DASVII010000187 DASVII010000000
VERSION     DASVII010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404392"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VF309_02400"
     CDS             complement(<1..908)
                     /locus_tag="VF309_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX7465462.1"
                     /translation="MANRYILPLETLRMTDVPRVGGKNASLGELISQLAARGVRVPGG
                     FATTAEAFNDFLAHNKLAPAIDAMLAKVDVENMASLAGAGMQIRALVEKGALQPELEK
                     QIRDDYARVSAGAPNASFAVRSSATAEDLPDASFAGQQETYLNIRGVENVLEAVRRVY
                     ASLYNDRAIAYRVHKGYSHGQIAISAGVQRMVRSDLGTAGVMFTLDTESGFREVVFIT
                     ASYGLGETVVQGAVNPDEFYVSKLCLFNGKPAILRRSLGTKAIKLIFDPAAQAGKSVT
                     EVEVPQADRDRFSLSDAEVEELARYAV"
     gene            982..>1792
                     /locus_tag="VF309_02405"
     CDS             982..>1792
                     /locus_tag="VF309_02405"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX7465463.1"
                     /translation="MSQRKRVFFVSDRTGITVEALGSSLLTQFADVDFDRLTLPFIDS
                     VDKARDVVAQVNAALRESGQRPLVFSSIVDDAVRTEINKADGLVLDVFERFIVPLENE
                     LGTKSTHAVGKSHSVSDVKDYNHRIEAINYTLSHDDGVTHRGLAEADIVLVGVSRSGK
                     TPTCLYLAMQFGIKAANYPLIPEDLEANRLPPSLLPLKQKVWGLSISPERLHHIRTER
                     RPDSTYAALENCRYEVAAAEKLMRQAGIPFMDSTTKSIEEIATHMLHEAHLV"
BASE COUNT          306 a          631 c          563 g          292 t
ORIGIN      
        1 accgcgtagc gcgcgagctc ctcgacctcg gcatcggaga gcgagaagcg gtcgcggtcc
       61 gcctgcggca cctcgacctc ggtcacggat ttccccgcct gcgccgcggg gtcgaagatg
      121 agcttgatcg ccttggtgcc gaggcttcgc ctgaggatcg cgggcttgcc gttgaagagg
      181 cagagcttgg agacgtagaa ctcgtccgga ttcaccgcgc cctgcaccac cgtctcgccg
      241 agcccgtagc tcgcggtgat gaagaccacc tcgcggaagc ccgattccgt gtccagggtg
      301 aacatcacgc ccgcggtccc gagatcgctc ctcaccatgc gctgcacgcc ggcggagatc
      361 gcgatctgcc catgggagta acccttgtgc acgcgatagg cgatggcgcg atcgttgtag
      421 agcgatgcgt acacgcggcg cacggcctcc agcacgttct cgacgccgcg aatgttgaga
      481 taagtctcct gctgtcccgc gaaggacgcg tcgggaaggt cctcggcggt cgccgaggag
      541 cgcaccgcga acgaggcgtt gggcgctccc gccgaaaccc gcgcgtagtc gtcgcggatc
      601 tgcttctcga gctccggctg cagcgcgccc ttctcgacga gcgcacggat ctgcatgccc
      661 gcgccggcga gggaggccat gttctcgacg tccaccttcg ccagcatcgc gtcgatcgcg
      721 ggggcgagct tgttgtgggc gaggaaatcg ttgaacgcct cggcggtcgt ggcgaatccc
      781 ccgggcaccc gcacccccct cgccgcgagc tggctgatga gctccccgag ggaggcgttc
      841 ttgccgccca cccggggaac gtcggtcatg cgcagcgttt cgagcgggag gatgtagcgg
      901 ttcgccatgg ggaatcgatt cggatgtggc aagctggagg ggataagcaa ttataacgaa
      961 cggttggcat ccactccccc catgtcccag cgaaaacgcg tcttcttcgt ctccgaccga
     1021 accggcatca ccgtggaagc gctcggctcg tccctcctca cccagttcgc cgacgtggat
     1081 ttcgaccggc tgacgctccc cttcatcgat tccgtggaca aggcgcgcga tgtcgtggcg
     1141 caggtgaacg cggcgctgcg cgagtcgggg cagcgccccc tcgtcttcag ctcgatcgtg
     1201 gacgacgcgg tgcgcaccga gatcaacaag gccgacgggc tggtgctcga cgtcttcgag
     1261 cgcttcatcg tccccctcga gaacgagctc ggcaccaagt cgacgcacgc cgtgggcaag
     1321 tcccacagcg tctccgacgt gaaggactac aaccatcgca tcgaggcgat caactacacg
     1381 ctctcccacg acgacggcgt gacgcaccgc ggcctcgcgg aggcggacat cgtgctggtg
     1441 ggcgtctcga ggagcgggaa gactcccacc tgcctctacc tcgcgatgca gttcggcatc
     1501 aaggccgcga actatccgct gatccccgag gacctcgagg ccaatcgcct gccgccctcg
     1561 ctcctgcccc tgaagcagaa ggtctggggc ctgtcgatct cgcccgagcg cctccaccac
     1621 atccgcaccg agcggcgccc cgactcgacc tacgccgcgc tcgagaactg ccgctacgaa
     1681 gtcgccgccg ccgaaaagct catgcgccag gccgggatcc ccttcatgga ctccaccacc
     1741 aagtccatcg aggaaatcgc cacccacatg ctgcacgagg cgcacctcgt cc
//
LOCUS       DASVII010000188         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_188560,
            whole genome shotgun sequence.
ACCESSION   DASVII010000188 DASVII010000000
VERSION     DASVII010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188560"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF309_02410"
     CDS             <1..275
                     /locus_tag="VF309_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7465464.1"
                     /translation="DLGDASGAESAFARAVAVDPAHARGWNNLAHARLDQGRLDEAEQ
                     ALKRALAAQPDHALAFLNLARVERSRGNAELARKYDGLAEGLGKAP"
     gene            complement(272..1372)
                     /locus_tag="VF309_02415"
     CDS             complement(272..1372)
                     /locus_tag="VF309_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7465465.1"
                     /translation="MNVGILALAVLLGAAASCHAQSKLPIDRIKVPPGFEVTVFAEGV
                     ENARSMALGEKGTLFVGSTGAGNVYAIKHDGRKATQVTKIATRLNTPNGVAVKDGALY
                     VAEIDKVWRYDDIESTLEAPRRTLVYGKYPTDRQHGWKFIRFGPDGWLYVPVGAPCNI
                     CDREAPYASITRLAPDGSAMEVFARGIRNSVGFDWDPDTKELWFTDNGRDMMGDDVPS
                     DELNHAPKAGMHFGYPYCHEGDVADPEFGARRKCTEFTPPARKLGAHVASLGMRFYTG
                     SMFPAEYRNRIFIAEHGSWNRSKKSGYRVVTVKIEGGKAVEHTVFAEGFLDTASDKAW
                     GRPVDVQVMPDGALLISDDTANAIYRIAYRSR"
     gene            complement(1369..>1679)
                     /locus_tag="VF309_02420"
     CDS             complement(1369..>1679)
                     /locus_tag="VF309_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX7465466.1"
                     /translation="YDGPVEVRDWAAVIHQKSPQAPPEPDRVTMYSTDWCPHCKNARN
                     YFTAKGIRFTEYNVETSETGRKEYEALGGGGVPVILVGSKVMRGFSPRSFEALARKSS
                     "
BASE COUNT          244 a          560 c          583 g          292 t
ORIGIN      
        1 tggacctggg cgatgcgagc ggcgcggaga gcgcgttcgc gcgcgccgtc gcggtcgacc
       61 ccgcccacgc gcgtgggtgg aataacctcg cccacgcgcg gctcgaccag ggccgcctcg
      121 acgaagccga acaggcgctc aagcgcgcgc ttgccgcgca gcccgaccat gccctcgcct
      181 tcttgaacct ggcccgcgtc gaacgctcgc ggggcaatgc cgagctggcg cgcaagtacg
      241 acgggcttgc cgagggcctc gggaaggcgc cctagcggct tcgataggcg atgcggtaga
      301 tcgcgttggc ggtgtcgtcc gagatgagga gcgccccgtc gggcatcacc tgcacgtcca
      361 ccgggcggcc ccacgccttg tcgctcgcgg tgtcgaggaa gccctcggcg aagaccgtgt
      421 gctcgaccgc cttgccgccc tcgatcttca ccgtcaccac gcggtagccg ctcttcttcg
      481 agcggttcca cgagccgtgc tcggcgatga agatgcggtt gcggtattcg gcggggaaca
      541 tcgagcccgt gtagaagcgc atgcccaggg aggccacgtg cgcgccgagc ttccgcgcgg
      601 gcggcgtgaa ctccgtgcac ttgcggcgcg cgccgaattc cggatcggcg acgtcgccct
      661 catggcagta gggatagccg aagtgcatgc cggccttcgg cgcgtgattc agctcgtcgg
      721 acggcacgtc gtcgcccatc atgtcgcggc cgttgtcggt gaaccagagc tccttcgtat
      781 ccggatccca gtcgaatccc acgctgttgc ggatgccgcg cgcgaacact tccatcgccg
      841 agccgtcggg cgcgaggcgc gtgatgctcg cgtagggcgc ttcgcggtcg cagatgttgc
      901 agggcgcgcc caccgggaca tagagccagc cgtcggggcc gaagcggatg aatttccagc
      961 cgtgctgcct gtcggtcggg tacttgccgt agacgagcgt gcgcctgggc gcctcgaggg
     1021 tggactcgat gtcgtcgtag cgccagacct tgtcgatctc ggccacgtag agcgcgccgt
     1081 ccttcaccgc gacgccgttg ggcgtgttga ggcgagtcgc gatcttcgtg acctgcgtgg
     1141 ctttccttcc atcgtgcttg atcgcgtaga cgttgcccgc gcccgtggag cccacgaaga
     1201 gggtgccctt ctcgccgagc gccatggagc gcgcgttttc cacgccctcg gcgaacaccg
     1261 tcacctcgaa gccgggcggc accttgatgc gatcgatcgg gagcttcgac tgcgcgtggc
     1321 aggaggccgc ggcgcccagc aggaccgcga gggcgaggat gccaacgttc atgaggattt
     1381 tcgtgcgagc gcctcgaaag acctgggaga aaaaccgcgc atgactttcg agcccaccag
     1441 gatcaccggg acgccgccgc cgccgagggc ttcgtattcc ttgcgcccgg tttccgaggt
     1501 ctcgacgttg tactcggtga atcggatgcc cttcgcggtg aaatagttcc gcgcgttctt
     1561 gcagtgcggg caccagtcgg tggagtacat ggtgacgcgg tcgggctcgg gcggcgcctg
     1621 cggcgatttc tggtggatca ccgccgccca gtcgcgcact tccaccgggc cgtcgtatg
//
LOCUS       DASVII010000189         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_440490,
            whole genome shotgun sequence.
ACCESSION   DASVII010000189 DASVII010000000
VERSION     DASVII010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440490"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VF309_02425"
     CDS             <1..595
                     /locus_tag="VF309_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7465467.1"
                     /translation="YGHILTVEDPIEFVHESKKCLMNQREVGPHTLSFSNALRSALRE
                     DPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTVDRIIDVFPAAEKEMIR
                     AMLSESLRAVISQTLCKTKDGTGRVAAHEIMIGTPAIRNLIREAKVAQMYSSIQTGQA
                     LGMQTLDQNLTEMVKRNLISMPEARNKAANKDNFVGG"
     gene            624..1763
                     /locus_tag="VF309_02430"
     CDS             624..1763
                     /locus_tag="VF309_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7465468.1"
                     /translation="MEREQSLKFMHDLLRALIARRGSDLFITAGFPPAMKVDGKVTPV
                     SQQSLSPMHTQELARSIMTDKQAAEFEATNECNFAISPSGIGRFRVNAFVQQGRSGLV
                     LRTITTAIPKFDELGLPHVLKDVAMTKRGLVIFVGGTGSGKSTSLASMIGHRNENSYG
                     HIITIEDPVEYVHEHKNCIITQREVGVDTDTWFAALKNTLRQAPDVILIGEIREKETM
                     EYGIAFAETGQLCMSTLHANSTNQALDRIINFFPEERRQQLLMDLSLNLKAIISQRLI
                     PKKGSKGRVAAIEIMLNSPLMSDLIFKGEVHEMKALMTRSRELGMQTFDQALFDLFEE
                     DMITFEDALRNADSVNDLRLKIKLESKNAKNRDLGSGLEHLEIVK"
BASE COUNT          387 a          613 c          561 g          272 t
ORIGIN      
        1 atacggccac atcctcacgg tcgaggatcc gatcgagttc gtgcacgaat cgaagaagtg
       61 cctcatgaac cagcgcgagg tcgggccgca caccctgtcg ttctccaacg cgctgaggag
      121 cgcgctgcgc gaggacccgg acatcatcct cgtgggcgag atgcgcgacc tggagacgat
      181 ccgcctcgcg atgaccgcgg cggagaccgg ccacctcgtg ttcggcacgc tgcatacgtc
      241 gtcggccgcc aagaccgtcg accgcatcat cgacgtcttc cccgcggcgg aaaaggagat
      301 gatccgcgcc atgctttcgg aatcgctgcg cgcggtgatc tcgcagacgc tttgcaagac
      361 caaggacggg accggccggg tcgccgcgca cgagatcatg atcggcacgc ccgccatccg
      421 caacctgatc cgcgaggcga aggtcgcgca gatgtactcg tcgatccaga ccgggcaagc
      481 gctcggcatg cagacgctcg accagaacct caccgagatg gtgaagcgca acctcatctc
      541 tatgcccgag gcgcgcaaca aggccgccaa caaggacaat ttcgtcggcg gctaacgcgg
      601 gcgcctaacc agaggacacg cgaatggaac gcgaacaatc actgaaattc atgcacgacc
      661 tgctgcgtgc gctcatcgcg cgcaggggct ccgacctctt catcaccgcg ggcttccctc
      721 cggcgatgaa ggtcgacggc aaggtcacgc cggtctccca gcagagcctg tcgccgatgc
      781 acacgcagga gctcgcccgc tcgatcatga ccgacaagca ggcggccgag ttcgaggcga
      841 ccaacgagtg caacttcgcc atcagccctt cgggcatcgg gcgcttccgc gtgaacgcgt
      901 tcgtgcagca gggccgctcg gggctggtgc tgcgcaccat caccaccgcg atcccgaagt
      961 tcgacgagct gggcctgccc cacgtgctga aggacgtggc gatgaccaag cgagggctcg
     1021 tgatcttcgt gggcggcacg ggctcgggca agtcgacctc gctcgcctcg atgatcggcc
     1081 accgcaacga gaacagctac ggccacatca tcacgatcga ggacccggtc gaatacgtgc
     1141 acgagcacaa gaactgcatc atcacgcagc gcgaggtggg cgttgacacc gacacctggt
     1201 tcgccgcgct gaagaacacg ctgcgccagg cgcccgacgt gatcctcatc ggcgagatcc
     1261 gcgagaagga gacgatggaa tacgggatcg ccttcgcgga gaccggccaa ctgtgcatgt
     1321 cgacccttca cgcgaactcc accaaccagg cgctcgaccg catcatcaac ttcttccccg
     1381 aggagcggcg ccagcagctg ctgatggacc tgtcgctcaa cctcaaggcg atcatctcgc
     1441 agcgcctcat cccgaagaag ggaagcaagg ggcgcgtcgc cgcgatcgag atcatgctca
     1501 actcgccgct catgtccgac ctcatcttca agggcgaggt gcacgagatg aaggcgctca
     1561 tgacccgctc gcgcgagctc ggcatgcaga ccttcgacca ggcgctgttc gacctcttcg
     1621 aggaagacat gatcactttc gaagacgcgc tgaggaacgc cgactcggtg aacgacctgc
     1681 gcctcaagat caagctcgag agcaagaacg cgaagaaccg cgacctgggc tcgggcctcg
     1741 agcacctcga gatcgtcaag tgatcgggcg gtagcgggcc aagcgatccg tttgcgcggt
     1801 catccccgcg aaggcgagga tccaatccag gaa
//
LOCUS       DASVII010000190         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_9367, whole
            genome shotgun sequence.
ACCESSION   DASVII010000190 DASVII010000000
VERSION     DASVII010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9367"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VF309_02435"
     CDS             <1..356
                     /locus_tag="VF309_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7465469.1"
                     /translation="SCRTLGVPARYVSGYLAADRNAQLASHAWVEAWVPNEGWQGYDI
                     ANNTKPGVRHVRVAVGLDYLDACPVRGYRRGGRGESMAVEVQVFDGRRARQAKQSQQS
                     QESKQQAQQAQQSQQ"
     gene            complement(366..1430)
                     /locus_tag="VF309_02440"
     CDS             complement(366..1430)
                     /locus_tag="VF309_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7465470.1"
                     /translation="MKAFGVGLVGYGLAGKAFHGLLIKATKGLEVRAISTSSAERSAQ
                     ARADFPDARVVAGFDELLQVAGLDAVVLGTPHDTHKDLALRALGRGLHVVTDKIMCLG
                     VREADQMIEAALRAGKMLSVFHNRRWDSDFLTLRACLAERLVGEPCAIESAVLRAGPD
                     ATPAPPPEQRPWRYRADKGGGPFRDWGAHLMDQAMILGGGMPESVYCDLQFRRPKLDV
                     ETLATCTMRFASGLRVRIEAGAHSWIQRPRWYVQGTEGTLKMEGLDPQEAWLRKGEVV
                     ARTERAKFDAAHTEVVKVAGTAPLEIVPGDYLAFYENIAAVLAGEAALAVTPESCRDV
                     LRVYEAAFESARRNEVVRIS"
     gene            complement(1427..>1875)
                     /locus_tag="VF309_02445"
     CDS             complement(1427..>1875)
                     /locus_tag="VF309_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7465471.1"
                     /translation="FDAVRIIGDALLLLRHDLRAKNCEMSFEHPGGGVDLSGVPGRLV
                     QVVSNLVGNAIDASAGRGGGPIRVELTPHEQGVDLRISDSGSGISSHVLPKIFEPMFT
                     TKPFGEGTGLGLTIVHDIVTGEFGGTIDVTSREGQGTTFILRFPRP"
BASE COUNT          320 a          643 c          612 g          300 t
ORIGIN      
        1 cgtcttgtcg aacgctgggc gtgcccgcgc gctacgtgag cgggtacctc gccgccgatc
       61 gcaacgcgca gctcgcgagc cacgcctggg tcgaagcgtg ggtccccaac gaaggatggc
      121 agggctacga catcgccaac aacacgaaac ccggcgtgcg ccacgtgcgt gtcgccgtcg
      181 ggctcgacta cctcgacgct tgccccgtgc gcggctaccg gcgcgggggc cgcggcgaat
      241 ccatggccgt cgaggtgcaa gtgttcgacg gccgccgcgc gcgtcaggcg aagcagtccc
      301 agcagtcgca agaatcgaag cagcaggccc agcaggcgca acagtcacag caatgagacc
      361 gcgggtcagc tgatccggac cacctcgttg cggcgcgccg attcgaaggc tgcctcgtag
      421 acgcgcagca catcgcggca gctttcgggt gtcaccgcaa gcgccgcttc cccggcgagc
      481 accgcggcga tgttctcgta aaacgcgagg tagtcgccgg gcacgatctc gagcggcgcc
      541 gtgcccgcga ccttgaccac ctcggtgtgg gcagcgtcga acttggctcg ctccgttcgt
      601 gcaacgacct cgcccttgcg cagccacgcc tcttgcggat cgaggccttc catcttgagc
      661 gtgccctcgg tgccctgcac gtaccagcgc gggcgctgga tccacgaatg tgcgcccgcc
      721 tcgatgcgca cgcgcagccc gctcgcaaag cgcatggtgc acgtggcaag ggtttcgacg
      781 tcgagcttcg ggcgccgaaa ttgcagatcg cagtagaccg attcgggcat gccgccgccg
      841 aggatcatcg cttgatccat gaggtgtgct ccccaatcgc gaaacgggcc tcctcccttg
      901 tcggcccggt atcgccaggg ccgctgctcg ggaggagggg cgggcgtcgc gtcaggtccc
      961 gcgcgcagaa cggcgctttc gatggcgcag ggctcgccca cgagccgctc ggcgaggcac
     1021 gctcgcaggg tcaaaaaatc gctgtcccag cggcggttgt gaaaaacgct cagcatcttg
     1081 ccggcccgca gcgccgcctc gatcatctgg tcggcctctc ggaccccgag gcacatgatc
     1141 ttgtcggtga cgacgtggag gcctctgccg agagcacgca gggcgaggtc cttgtgcgta
     1201 tcgtgcggcg ttccgagaac gacggcatcg agccccgcta cctggagcag ctcgtcgaaa
     1261 cctgcaacca cacgagcatc cgggaagtcg gcgcgcgcct gggcgctgcg ctccgcggaa
     1321 gacgtggaga tggcccgcac ctcgagcccc ttcgtcgcct tgatcaagag cccgtggaac
     1381 gccttgcctg ccaacccgta cccgaccaac ccgactccaa acgctttcat ggtcgcggaa
     1441 accgcaggat gaacgtcgtc ccctgccctt cccggctcgt gacgtcgatc gttcccccaa
     1501 actcgccggt gacgatgtcg tggacgatgg tgaggccgag cccggttccc tcgccgaacg
     1561 gcttggtggt gaacatgggc tcgaagatct tgggcaagac gtgggagctg attcccgatc
     1621 cgctgtcgga gatgcggagg tcgacacctt gttcgtgggg ggtcagctcg acacgaatcg
     1681 gacctccccc gcgaccggcg ctggcatcga tggcgtttcc gaccaggtta ctcaccacct
     1741 ggaccagacg accgggaacc ccggacaagt cgaccccgcc gccagggtgc tcgaagctca
     1801 tttcgcagtt tttcgcccgg agatcgtggc gtaggagcag aagcgcgtcc ccgatgatgc
     1861 gaacggcatc gaagc
//
LOCUS       DASVII010000191         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_548688,
            whole genome shotgun sequence.
ACCESSION   DASVII010000191 DASVII010000000
VERSION     DASVII010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548688"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VF309_02450"
     CDS             complement(<1..814)
                     /locus_tag="VF309_02450"
                     /inference="COORDINATES: protein motif:HMM:NF004957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX7465472.1"
                     /translation="MLNIDLSGRRALVAGVADDGGFGFAIAKSLAEAGASVVVGTWPP
                     ALNIFLNLLERGKMDESRQLGDGSLLQFEKIYPLDAVYDTLDSAPEELRTSKRYKEVG
                     DFSIDGMASQLVKDFGERPLDIVIHSLANGPEVKKPLLETSRNGYLAAVSASAYSLVA
                     MVARLGPLMRAGGSVASLTYMASERVIAGYGGGMSSAKAALESDTRTLAYEAGRKWGI
                     RVNTVSAGPYASRAASAIGIIGRMVEYVGKNAPLTDHLTAEEVGATMAFLSSS"
     gene            900..>1529
                     /locus_tag="VF309_02455"
     CDS             900..>1529
                     /locus_tag="VF309_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX7465473.1"
                     /translation="MEADLPAARSTAVGARAEHSASAVPADERRLTPPGSGPAFARGL
                     WRIAAADVSSGNSVELLHDGPATFDAMCAAIATARETVVLEAYILRSDETGHRIANAL
                     IDAAGRGVRIRVLSDWIGMRGIKSSYLHRLGKAGVEHRVFSPPGFRPWFGLIPRDHRK
                     LLVIDSAVGITGGVGIGDEWVHGKTKRNRKHWRDTVVRIEGPAALDMQQA"
BASE COUNT          233 a          517 c          539 g          240 t
ORIGIN      
        1 gcgacgagct gaggaacgcc atcgtggcgc caacctcctc ggcggtgagg tgatcggtga
       61 gcggcgcgtt cttccccacg tactccacca tcctgccgat gatgccgatg gcgctcgccg
      121 cgcgcgacgc ataggggccg gcggacacgg tgttcacgcg aattccccat ttccgccccg
      181 cttcgtaggc gagcgtgcgg gtgtcgctct cgagcgcggc cttggcgctg gacatcccgc
      241 cgccgtagcc ggcaatgact cgctcgcttg ccatgtacgt gagcgacgcc acgctgccgc
      301 cggcgcgcat gagtgggccg agcctggcca ccatggccac cagcgagtag gccgaggcgc
      361 tcaccgcggc gagatagccg ttgcggctcg tctcgaggag cggcttcttc acctccgggc
      421 cattggccag cgaatggatg acgatgtcga gcggccgctc gccgaagtct ttcacgagct
      481 gggaggccat cccatcgatg gagaagtcgc ccacctcctt gtagcgcttg ctcgtgcgca
      541 gctcctcggg tgcggagtcg agcgtgtcgt acacggcgtc gagggggtag atcttctcga
      601 actgcaggag cgagccgtcg ccgagctggc gggactcgtc catctttcca cgttccagaa
      661 ggttgaggaa gatgttgagc gctggaggcc aggtgccgac cacgacactg gccccggcct
      721 cggcgagcga tttggcgatg gcgaagccga agccgccgtc gtcggcaacg ccggcgacga
      781 gcgcgcggcg tccggagaga tcgatgttga gcatcgcgaa taatgacccc gcgcgcccgg
      841 atgcgggagg acggcccgcc aagtaacctt cacgtgtgcg cacaccgctt gaccccgccg
      901 tggaagccga ccttcccgcc gcgcgctcca ccgccgtagg ggcgcgcgcc gagcactccg
      961 cttcggccgt accggccgac gagcgccggc tcacgccgcc gggcagcggg ccagcctttg
     1021 cgcgcggcct ctggcgcatt gccgcggcgg acgtgtccag cggcaattcc gtggagctgc
     1081 tgcacgacgg gcccgccacc ttcgatgcca tgtgcgcggc catcgccacg gcgcgcgaga
     1141 cggtggtgct ggaggcgtac atcctccgct ccgacgagac gggccaccgc atcgcgaatg
     1201 cgctcatcga tgcggcggga cgcggggtgc ggatccgcgt gctctcggat tggatcggga
     1261 tgcgcggcat caagagcagc tatctccacc ggctcggcaa ggcgggggtg gagcaccggg
     1321 tgttcagtcc gcctggcttt cgcccctggt tcgggctcat tccgcgcgac caccgcaagc
     1381 tgctggtcat cgattccgcg gtggggatca ccggcggtgt gggcatcggc gacgagtggg
     1441 tgcacggcaa gacgaagcgc aaccgcaagc actggcgcga tacggtggtt cgcatagagg
     1501 gacccgccgc gctcgacatg cagcaggcg
//
LOCUS       DASVII010000192         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_117258,
            whole genome shotgun sequence.
ACCESSION   DASVII010000192 DASVII010000000
VERSION     DASVII010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117258"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF309_02460"
     CDS             <1..273
                     /locus_tag="VF309_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7465474.1"
                     /translation="LQLLKHFNIDPILWGTLVFVNLQAAFLSPPVAMSAFYLKGVSPK
                     HVTLNQIFAGMMPYMLIVILCMVIMYLWPGMTLWLPNYLYGNAGGS"
     gene            complement(329..733)
                     /locus_tag="VF309_02465"
     CDS             complement(329..733)
                     /locus_tag="VF309_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465475.1"
                     /translation="MSTLFGVGIFVGFTVGIWFSYRLFMRGDTYAEKVARLLPPDFKP
                     DVFHRKGDTYVGYEKGRNRLVLVDWPHASVLSPNEVVSIDPVHESTLGVTHHWVSVGV
                     PGAVFSPYRIWFQFRRDKRDAWLGQLATICGK"
     gene            827..>1530
                     /locus_tag="VF309_02470"
     CDS             827..>1530
                     /locus_tag="VF309_02470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7465476.1"
                     /translation="MRKILITRAVFPEVVAALSQRFEVVHNAEDRPWPPEELARRLEG
                     VSGAMATVMDRFDEPILAQCAQLKVISNIAVGYNNIDVPACTRRGIRVTNTPGVLDDT
                     TADLTWSLLMAAARRIAEADAYVRRGDWKIAFGVQYFLGQDIHHATLGIIGMGRIGQA
                     IARRASGFDMRVLYNNRSRLPEADEKRLDATRVERDELLAQSDFVVVMVPYSPATHHM
                     IGAAEIAKMKPNAILV"
BASE COUNT          277 a          510 c          472 g          271 t
ORIGIN      
        1 ctgcagctgc tcaagcactt caacatcgac ccgatcctct ggggcacgct cgtgttcgtg
       61 aacctgcagg cggcgttcct gtccccgccc gtggcgatgt cggccttcta tctcaagggc
      121 gtctcgccga agcacgtgac gttgaaccag atcttcgcgg gcatgatgcc ctacatgctg
      181 atcgtgatcc tgtgcatggt gatcatgtac ctgtggcccg gcatgacatt gtggctgccc
      241 aactacctct acgggaacgc gggcggcagc tgaacgacgg aaaaacaggc gcggagacgc
      301 gcctgttttt cgcggctact tcccgaagct atttcccgca gatggtcgcg agctgcccca
      361 gccacgcgtc gcgcttgtcg cggcggaact ggaaccagat ccggtacggc gagaacaccg
      421 cgcccggaac tcccacggag acccaatgat gcgtcacgcc cagcgtcgat tcgtgcacgg
      481 gatcgatcga cacgacttcg ttgggcgaaa ggacgctcgc gtggggccag tcgacgagca
      541 cgaggcggtt gcgccccttc tcgtatccca cgtaggtgtc gcccttgcga tggaagacgt
      601 cgggcttgaa atccggcggc aggaggcggg cgaccttctc cgcgtaggtg tcgccgcgca
      661 tgaacaagcg atatgaaaac cagatgccga cggtgaaccc gacgaagatt ccgactccga
      721 acaatgtgct catcatgccc gccacccttt ccgtgtcaat tgaccatgct aagctgaacc
      781 cgcctcaggc gctcgggtat ttccggcgct tccgggtcct cattccatgc gcaagatcct
      841 gatcacgcgt gccgtgtttc ccgaggtcgt cgcggcgctt tcgcagcgct tcgaggtcgt
      901 ccacaacgcc gaggatcgcc cgtggccgcc ggaagagctc gcgcggcgcc tcgagggcgt
      961 gagcggcgcg atggccacgg tgatggaccg cttcgacgag cccatcctcg ctcaatgcgc
     1021 gcagctcaag gtgatctcca acatcgcggt cgggtacaac aacatcgacg ttccggcgtg
     1081 cacgcggcga ggcattcgcg tgacgaacac acccggcgtc ctcgacgaca ccaccgccga
     1141 tctcacgtgg tccctgctga tggccgcggc gcgccgcatc gcggaggccg acgcgtacgt
     1201 gcgccggggc gattggaaga tcgctttcgg cgtgcagtac ttcctcgggc aggacattca
     1261 ccacgcgacg ctcggcatca tcggcatggg gcgcatcggc caggcgatcg cgcggcgcgc
     1321 atcgggattc gacatgcgcg tgctctacaa caatcgctcg cgccttcccg aagccgacga
     1381 gaagcgcctc gacgcgacgc gcgtcgagcg cgacgagctc ctcgcgcagt cggacttcgt
     1441 cgtggtgatg gttccctact cgccagcgac ccatcacatg atcggcgccg ccgagatcgc
     1501 gaagatgaaa cccaatgcga tcctcgtgaa
//
LOCUS       DASVII010000193         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_440645,
            whole genome shotgun sequence.
ACCESSION   DASVII010000193 DASVII010000000
VERSION     DASVII010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440645"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VF309_02475"
     CDS             <1..372
                     /locus_tag="VF309_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7465477.1"
                     /translation="AVDYVGVANLFTFMNTIPPYWRPFLPKFQDMVGHPEKDAERMRA
                     TSPVLHADRIKTPLFIAQGARDPRVNKAESDQVVEALRKRGVEGQYMVKDNEGHGFRN
                     EENQFDFYAAMEKFFAEHLHP"
     gene            471..>1562
                     /locus_tag="VF309_02480"
     CDS             471..>1562
                     /locus_tag="VF309_02480"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7465478.1"
                     /translation="MRLLAAALALLTTTAAFAQPLPTAPPEQLGFSSEGLARIDRFFA
                     REIAQNRVPGAIVAIARDGKLVYFKSFGYLDKAAGTPMPLDAIFNLASMTKPMTAVAG
                     LTLNEEGRLPLKSRLDEYFPAFSDMKVGVAGADGKAEKAKPIFVQDLFRHTTGLTYGA
                     RGDTAVHKLYPPGSAPAAARYSGAEFAAQLATLPLLYQPGTVWDYGFSIDMLGLVIEK
                     ITGERLGQYLQAAVWDKVGMPDTTFQVPAQKRKRLARPLPNDPLTGKEQKIAILDEPV
                     KFDCAGSCAFSTVSDYLRFGQMLLDGGAIDGHRVLSPKTVAMMTSDQLGPGIKSQVGG
                     TEQQREGYGFGYGVAVRVHEGIASVPGSVG"
BASE COUNT          279 a          541 c          512 g          230 t
ORIGIN      
        1 gcggtggact acgtcggcgt cgcgaacctc ttcacgttca tgaacaccat cccgccgtac
       61 tggcgcccgt tcctgccgaa attccaggac atggtgggac accccgagaa ggacgccgag
      121 cgcatgcgcg ccacttcgcc cgtgctgcac gccgatcgca tcaagacccc gctgttcatc
      181 gcgcagggcg cgcgcgaccc gcgcgtgaac aaggcggaga gcgaccaggt ggtcgaggcg
      241 ctgcgcaagc gcggcgtcga ggggcagtac atggtgaagg acaacgaggg ccacggcttt
      301 cgcaacgagg agaaccagtt cgacttctac gcggcgatgg agaaattctt cgcggagcac
      361 ctgcatccct agcggttcaa gacggtcacg ccgccacgcc gcgtgatcgt cttgtcagcg
      421 ccagcggcaa gagtcaataa tcggggaata aaccacacga ggagaacccg atgcgtttgc
      481 tcgcggccgc actggcattg ctgaccacca cggcggcgtt cgcgcagccc cttcccacgg
      541 cgccgccgga gcagctcggc ttctcgagcg aggggctcgc gcgcatcgac cgcttcttcg
      601 cgcgcgagat cgcgcagaac cgcgtccccg gcgcgatcgt cgccatcgcg cgcgacggca
      661 agctcgtcta cttcaagtcg ttcggatacc tcgacaaggc cgcgggcacg ccgatgccgc
      721 tcgacgcgat cttcaacctt gcctcgatga cgaagccgat gaccgcggtg gcgggcctca
      781 cgctgaacga ggaaggacgc ctgccgctca agtcgcggct cgacgagtat ttccccgcgt
      841 tctccgacat gaaggtcggc gtggcgggcg ccgacggcaa ggcggagaag gcgaagccca
      901 tcttcgtcca ggatctcttc cgccacacga ccggcctcac ctacggcgcg cgcggcgaca
      961 cggccgtgca caagctctac ccgccgggat ccgctcccgc ggccgcgcgc tattcgggcg
     1021 cggaattcgc cgcgcagctc gccacgctcc cgctccttta ccagcccggc accgtatggg
     1081 actacggctt ctcgatcgac atgctgggac tcgtgatcga gaagatcacg ggagagcggc
     1141 ttggccaata cctgcaggcc gccgtgtggg acaaggtcgg catgcccgat acgacgttcc
     1201 aggttccggc gcagaagcgc aagcgcctcg cgcgcccgct ccccaacgac ccgctcacgg
     1261 gcaaggagca gaagatcgcg atcctcgacg agccggtgaa gttcgactgc gccggctcgt
     1321 gcgccttcag caccgtgagc gattacctgc gcttcggcca gatgctcctc gacggcggcg
     1381 cgatcgacgg gcatcgcgtg ctgagtccga agaccgtggc gatgatgacg agcgaccagc
     1441 tcggcccggg catcaagagc caggtcggcg gcacggagca gcagcgcgag ggctacggct
     1501 tcggctacgg cgtcgcggtg cgcgtgcacg aaggcatcgc ctcggtcccc ggcagcgtcg
     1561 gg
//
LOCUS       DASVII010000194         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_369114,
            whole genome shotgun sequence.
ACCESSION   DASVII010000194 DASVII010000000
VERSION     DASVII010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369114"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VF309_02485"
     CDS             <1..117
                     /locus_tag="VF309_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX7465479.1"
                     /translation="HTAIQMDGYKSLKEAQRVSFEVRTGPKGLQAADIKAID"
     gene            202..1533
                     /locus_tag="VF309_02490"
     CDS             202..1533
                     /locus_tag="VF309_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX7465480.1"
                     /translation="MTDRAADAARWRTALASNPNDRIAWHNLASAQGDLGRAAEAEAA
                     ARRAIELGIAAPETRLVLARALQAQRRLDEAEKAFDEAISLRPDYLDAQRDLAQLVWM
                     RTARSDLALARIDRGLRSAPRDAGLYLIRSIVLEIAGDRDAAFAAVVTGLNAAPRDVP
                     LLLQAAHLCAEMNEAARALQFARQADKIAPTNIAAGFGLCEALLAAGELDEADRIAAV
                     LCAAQPRNQYAIALQATAWRLGGDPRYGELYRYDTLVEARKLDTPAGWKSLDDFLHEL
                     KRELDAMHSYTSHPLQQSVRGGSQLPLNAPELEHPLVAALFASISGAVGRYIAGTGPK
                     GFAISGAWSVRLRSGGYHTDHVHPEGWISSACYIALPESVTSGTHFAGWLRFGKPGIA
                     TKPALAPDHYVKPEPGLLVLFPAYMWHGVEPFESPEPRLTVAFDVVPEDPR"
     gene            1530..>1718
                     /locus_tag="VF309_02495"
     CDS             1530..>1718
                     /locus_tag="VF309_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX7465481.1"
                     /translation="MIRILLAVIALLLSSAVGRYIAGAAARPFAISGAWSVRLRSGGF
                     HTAHVHPEGWISSACYIAL"
BASE COUNT          267 a          635 c          550 g          266 t
ORIGIN      
        1 cacaccgcga tccagatgga cggctacaag tccctcaagg aggcccagcg cgtttcgttc
       61 gaagtgcgca ccggccccaa gggactccag gctgccgaca tcaaggcgat cgactgatcg
      121 tcattggatt cccgtctaca cgggaatgac gactgaaagg gcccggcaca tgccgggcct
      181 ttttcattgc agaatgccgc catgaccgat cgagccgcgg acgccgcgcg ctggcgcacg
      241 gcgctggcct ccaacccgaa cgaccgaatc gcgtggcata acctcgcctc cgcgcagggc
      301 gacctcggcc gtgccgccga ggccgaagcc gccgcgcgcc gcgccatcga gctggggatc
      361 gcggcgcccg agacgcgcct tgttctcgcg cgcgctctac aggcgcagcg ccgcctggac
      421 gaggccgaga aggccttcga cgaggcgatc tcgctgcggc ccgactacct cgacgcgcaa
      481 cgcgatctcg cgcagctcgt ctggatgcgc accgcccgca gcgacctcgc cctcgcccgc
      541 atcgaccggg ggctacgctc cgcgccgcgc gatgccggcc tctacctcat ccgctcgatc
      601 gtgctcgaga tcgccggcga tcgcgacgcc gcgttcgccg ccgtggtgac gggcctgaac
      661 gccgcgccga gagacgtgcc cttgttgctg caggccgcgc atctctgcgc ggaaatgaat
      721 gaagcggcgc gcgcgctcca gttcgcgcgc caggccgaca agatcgctcc cacgaacatc
      781 gcggcgggat tcggcctgtg cgaggcgctg ctggccgcgg gcgagctcga cgaggccgac
      841 cgcatcgccg cggtcctttg cgcggcgcag ccccgcaatc aatatgcgat cgcgttgcag
      901 gcgacggcgt ggcggctggg cggcgatccg cgctacggcg agctctatcg ctacgacacg
      961 ctggtcgagg cgcgcaagct cgacacgcct gccggatgga agagcctcga cgacttcctt
     1021 cacgagctca agcgcgagct ggacgccatg cacagctata cgagccaccc gcttcagcaa
     1081 tcggtgcgcg gcgggagcca gcttccgctc aatgcaccgg agctcgagca tccgctggtg
     1141 gccgcactct ttgcgagcat ctcgggcgcg gtcgggcgat acatcgccgg gaccggcccc
     1201 aaaggcttcg ccatctccgg cgcgtggagc gtgcggctgc gctccggcgg ctatcacacc
     1261 gaccacgtgc atcccgaggg atggatctcg tcggcttgct acatcgcctt gccggaaagc
     1321 gtcaccagcg ggacgcactt cgcgggatgg ctgcgcttcg gcaagcccgg catcgcgacc
     1381 aagcccgcgc tcgctcccga ccactacgtg aagcccgagc cgggactcct cgtcctcttc
     1441 cccgcgtaca tgtggcatgg cgtggaaccc ttcgaaagtc ccgagccgcg cctcaccgtg
     1501 gcattcgatg tcgtcccgga ggatccccga tgatccgcat ccttcttgcc gtgatagcgc
     1561 tcttgctctc gagcgcggtc gggcgataca tcgccggggc cgccgccagg cccttcgcca
     1621 tctccggcgc atggagcgtg cgcctgcgct ccggcggttt ccacaccgcc cacgtgcatc
     1681 ccgaaggctg gatctcatcg gcgtgctaca tcgccctt
//
LOCUS       DASVII010000195         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_404739,
            whole genome shotgun sequence.
ACCESSION   DASVII010000195 DASVII010000000
VERSION     DASVII010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404739"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF309_02500"
     CDS             complement(<1..236)
                     /locus_tag="VF309_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEX7465482.1"
                     /translation="MERTLASPATLVEALLRPACYPHPVDRVEVLETHISYVLLAGEF
                     AYKIKKPVRLAFLDFSSLQARRFYCEEEVRLNRRT"
     gene            complement(239..544)
                     /locus_tag="VF309_02505"
     CDS             complement(239..544)
                     /locus_tag="VF309_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465483.1"
                     /translation="MKSRDEYVAKLKTDLDRWNVEAAKWEAQAASARADLKKGYTKQL
                     ETLRAQREATLYKLKLLEGASATAWTELERGADEAWASMKEAMAAAKSHFEKAHTAK"
     gene            648..1298
                     /locus_tag="VF309_02510"
     CDS             648..1298
                     /locus_tag="VF309_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7465484.1"
                     /translation="MYFDDRTDAARQLADALRKYRGRDPLVLAIPRGAVPMGCIIADA
                     LEGDFDVVLVRKLQAPSSGELAVGSVDESGWTYVADFARDLGVDDEYLEEEKRVQLDV
                     LKRRRALYTPWRRPLDPSGRIAIVIDDGLATGATMIAALHAVRSRHPATLVCAIPVAS
                     GESLVRIASLADDIVCLETPVGFQSVGQFYHAFDQVSDDTVTRILREREAVAISSP"
     gene            1295..>1951
                     /locus_tag="VF309_02515"
     CDS             1295..>1951
                     /locus_tag="VF309_02515"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7465485.1"
                     /translation="MSARPAIAAPAENEYVADRLREAAHLLEAQGASAYRVLAYRGAG
                     EAVAAQGRSVREIFESEGVRGLDAIPRVGLGIAAAIAEMLTTGRWSLLERLRGTTEPA
                     TLFQTVPGMGPALAARIHDRLHIDSLEALEAAAHDGRLRTVPGVGERRAAAWRAALDS
                     MLGRLRGGRPFAIADTAAEPAVATILDVDREYREKARAGKLRTIAPRRFNPEGHAWLP
                     L"
BASE COUNT          274 a          712 c          644 g          321 t
ORIGIN      
        1 gtgcgccgat tcaggcgcac ctcctcctcg cagtagaagc gccgcgcttg cagggacgag
       61 aaatcgagga aggcgaggcg aaccggcttc ttgatcttgt aggcgaattc tccggcgagc
      121 agcacatacg agatgtgcgt ctccagcacc tcgacccgat cgacgggatg cggatagcag
      181 gcaggccgca gcagcgcctc gaccagggtg gccggcgaag cgagggtccg ttccacggct
      241 acttcgcggt gtgcgccttc tcgaagtgcg acttcgccgc cgccatcgct tccttcatgc
      301 tcgcccacgc ctcgtcggcg ccgcgctcca gctcggtcca cgcggtagcc gatgcaccct
      361 ccagcagctt cagcttgtag agcgtcgcct cgcgctgcgc gcgcagggtc tccagctgct
      421 tggtgtagcc cttcttcagg tccgcgcgcg cgctcgcggc ctgcgcttcc catttcgcgg
      481 cctcgacgtt ccagcggtcc aggtcggtct tcagcttcgc tacatactcg tcgcggcttt
      541 tcatggcttc ctccagggtc gattcacacc tccattctcg atgcacggcc cgccgcgggc
      601 ttgattccgg tcaaaggccg ccccatggcg cgaccgacaa tggttccatg tacttcgacg
      661 accgcaccga cgctgccagg cagctggccg atgccctgcg caagtaccgc gggcgcgacc
      721 ccttggtcct ggccataccg cgcggcgccg tgccgatggg atgcatcatc gccgacgccc
      781 tcgaaggcga tttcgacgtc gtgctggtga ggaagctcca ggcgccctcg agcggggagc
      841 tcgccgtggg ttccgtcgac gaaagcggct ggacctacgt cgccgacttc gcgcgcgacc
      901 tgggtgtcga cgacgagtac ctggaggaag agaagcgcgt ccagctcgac gtgctcaagc
      961 gccgccgcgc gctctacacg ccttggcggc gccccctcga tccttcgggg cgcatcgcca
     1021 tcgtgatcga cgacgggctc gcgaccggcg ccaccatgat tgccgctctc cacgccgtgc
     1081 gctcgcgcca tcccgcgacg ctcgtttgcg cgattccggt cgcttccggc gaaagcctcg
     1141 tgaggatcgc gtccctcgcc gacgacatcg tctgcctcga gaccccggtc ggcttccagt
     1201 cggtggggca gttctaccat gccttcgacc aggtttccga cgacaccgtg acgcgcatcc
     1261 tgcgggagcg cgaggcggtg gcgatctctt ccccatgagc gcgcgccccg ccatcgcggc
     1321 gcccgccgag aacgaatacg tcgccgacag gctgcgcgag gcggcgcacc ttctcgaagc
     1381 ccagggcgcg agcgcctatc gcgtgctcgc gtatcgcggc gccggcgaag cggtggcggc
     1441 ccagggccgc agcgttcgcg agatcttcga gtccgagggc gtgcgcgggc tcgacgcgat
     1501 tccgcgcgtc ggattgggaa tcgcggcggc gatcgccgag atgctcacca ccggccgctg
     1561 gtcgctgctc gaaaggctgc gcggaaccac cgagcccgcc acgctgtttc aaacggtccc
     1621 cggcatgggc cccgccctcg ccgcgcgcat ccacgaccgc ctgcacatcg attcgctcga
     1681 ggctctcgag gccgcggctc acgatggccg gctgcgcacc gtgcccggcg tgggcgagcg
     1741 gcgcgccgcc gcgtggcgcg ccgcgctcga ttccatgctg ggaaggcttc gcggcgggcg
     1801 gcccttcgcg attgcggaca ccgcggccga gcctgcggtc gccacgatcc tcgacgtcga
     1861 ccgcgagtac cgggagaagg cgcgcgcggg gaagctgcgc accatcgcgc cgcgacgatt
     1921 caatcccgag ggccatgcgt ggcttccgct g
//
LOCUS       DASVII010000196         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_332986,
            whole genome shotgun sequence.
ACCESSION   DASVII010000196 DASVII010000000
VERSION     DASVII010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332986"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF309_02520"
     CDS             complement(<1..376)
                     /locus_tag="VF309_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465486.1"
                     /translation="MFSSTWPARLPLSSGLLAGKLSRATAFAAADHRGFNRGGAQFDR
                     GETFSGLDYRVGLEAVEALRPLVPAGQTLPQMAIRWILMNPAAQCWLAYRAQALRSYA
                     LATQRCAPSARAEGDSRAARGAC"
     gene            complement(939..1466)
                     /locus_tag="VF309_02525"
     CDS             complement(939..1466)
                     /locus_tag="VF309_02525"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7465487.1"
                     /translation="MKASPWVAAIAAAWIGAQAQAAANDPPFLIQCRLMQPNVPAVAP
                     IDAPFSRIVESASRAHGVEGRLVHALIWAESSYDPKAVSSSGAEGLMQLMPATAKRYG
                     VRDAFDPKQNIDGGVKHLRELLDQFDGDLELVVAAYNAGANAVIRAGNRIPPYPQTAA
                     YVPKVIAYYHHLAAR"
BASE COUNT          264 a          525 c          531 g          250 t
ORIGIN      
        1 cgcacgcgcc cctcgcggcg cgcgagtcac cttccgcgcg cgcggacgga gcgcaacgct
       61 gcgtcgcgag cgcgtacgac cgcaacgcct gcgcgcgata cgcgagccag cactgcgccg
      121 ccggattcat caggatccat cggatcgcca tctgcgggag cgtttgtccc gcgggaacga
      181 gcgggcgcag cgcctccacc gcctccaggc ccacgcgata gtcgaggccc gagaaggtct
      241 cgccgcggtc gaactgcgcg ccgccgcgat tgaagccgcg gtggtcggcg gcggcgaagg
      301 cggtcgcgcg cgaaagcttg ccggccagca gccccgagga gagcggcaag cgcgcgggcc
      361 aggtcgagga gaacatggcg gcggcggagt tgcccgcgct ttccgcgagc gcgatggcgg
      421 cgatcggcgc cgtgtacgac gactacgcgc gttcgcgcgt ccacgcgctc tggtgagagc
      481 cggcactcgt atcgacgcca tccatcgagg ccgggatgaa gcccgagccg gcgcggtcgt
      541 tcctggatgg gggagggagc tcgctactac ccatgtcgct cggattcgtt gagcttcttc
      601 ttccctgtgg ccgacagtgg gacgcgcctc tcgggcttca tcccaagggc tcgatggatc
      661 gctctgcgcg cacgcatcga ctgtcccaca agctttgaat ggactcgccc agaataacgg
      721 cgatgcgcga cgcccgtcat cgcaagcccc gaccagcaga gcctcattgg aagtgtaggg
      781 gtgaagctcg agaggaccgt cccactgccg gttccacggg agaagaagcc caacgaattc
      841 gagcggcatg ggtagtagcg agctctctcc cctatccagg aacgatcggg ccggctcgag
      901 cttcaccccc acttccaatg aggcgatagc ccagagaatc agcgagcagc gaggtggtgg
      961 tagtacgcga tgaccttcgg aacgtacgcc gccgtctgcg gatacggcgg aatgcgattg
     1021 cccgcccgga tcaccgcgtt ggcgcccgcg ttgtatgccg ccacgaccag ctcgaggtct
     1081 ccgtcgaatt ggtcgaggag ctcgcgcagg tgcttcacgc cgccgtcgat gttctgcttc
     1141 ggatcgaagg catcacgcac gccgtagcgc ttcgcggtcg ccggcatgag ctgcatcagc
     1201 ccctcggccc cggacgacga gaccgccttc gggtcatagg acgactcggc ccagatgagc
     1261 gcgtgcacca ggcgtccttc gacgccgtgg gctcggctgg cactctccac gatgcgtgaa
     1321 aatggcgcgt cgatgggcgc gacggccgga acgttcggct gcatcaggcg gcactggatg
     1381 agaaagggag gatcgttcgc ggcggcctgg gcctgcgctc cgatccacgc ggcggcgatc
     1441 gccgcgaccc acggggatgc tttcatggcg gacttccttt ttgctgcact gcacaaccca
     1501 ccagtttccg ggctttgccg aggagggtca acccacgatg caccagttat tttgtgccgt
     1561 gcagcagccc
//
LOCUS       DASVII010000197         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_548962,
            whole genome shotgun sequence.
ACCESSION   DASVII010000197 DASVII010000000
VERSION     DASVII010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548962"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1841
                     /locus_tag="VF309_02530"
     CDS             <1..>1841
                     /locus_tag="VF309_02530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380117.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7465488.1"
                     /translation="EAFNKSGIKPDWMVMEVLPVLPPDLRPLVPLDGGRFATSDLNDL
                     YRRVINRNNRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKS
                     LADMIKGKSGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKLMALELFKPFIFHK
                     LEIMGLATTIKAAKRMVEAQEPVVWDILEDVIREHPVLLNRAPTLHRLGIQAFEPVLI
                     EGKAIQLHPLVCAAFNADFDGDQMAVHVPISLEAQMEARTLMLSSNNVLHTAHGEPVI
                     VPSQDIVLGLYYATRERVNARGEGSFFVNISEVSRAYESHGVELHARITVRIKESRKN
                     DDGTLTEVVNRYETTVGRALMSEILPAGLPFSIINKPLKKKEISKLINEAFRRCGIRE
                     TVIFADKLMYTGFSFATRAGLSICVDDLLIPDEKAGIIEESEKQVKEIERQYTSGLVT
                     QGERYNKVVDIWGRSGDKVAKAMMDQLGNQKTTDRKGKEVTEESFNSIYMMADSGARG
                     SAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKT
                     ANSGYLTRRLVDVAQDAIIAEYDCGTMDGISLGALVEGGEIIEAMSER"
BASE COUNT          363 a          613 c          593 g          272 t
ORIGIN      
        1 gaggccttca acaagtcggg aatcaagccc gactggatgg tgatggaagt gctgcccgtg
       61 ctgcctccgg acctgcgtcc gctggtgccg ctcgacggcg gccgcttcgc gacctccgac
      121 ctgaacgacc tctatcgccg cgtgatcaac cgcaacaacc gcctgaagcg tctcctcgag
      181 ctgaaggccc ccgagatcat cgtgcgcaac gagaagcgca tgctgcagga agccgtcgat
      241 tcgctcctcg acaacggccg ccgcggcaag gcgatgacgg gcgcgaacaa gcgcgcgttg
      301 aagtcgctcg ccgacatgat caagggcaag agcggccgct tccgccagaa cctcctcggc
      361 aagcgcgtcg actactcggg ccgctcggtg atcgtcgtcg gacccacgct gaagctgcac
      421 cagtgcggcc tgccgaagct gatggcgctc gagctcttca agcccttcat cttccacaag
      481 ctcgagatca tgggcctcgc gaccaccatc aaggccgcca agcgcatggt cgaagcgcag
      541 gagcccgtgg tgtgggacat cctcgaggac gtgatccgcg agcacccggt gctgctgaac
      601 cgggcgccca cgctccaccg cctcggcatc caggcgttcg agccggtgct gatcgagggc
      661 aaggcgatcc agctgcatcc gctcgtctgc gccgcgttca acgcggactt cgacggcgac
      721 cagatggcgg tccacgttcc catctcgctc gaagcgcaga tggaagcgcg caccctcatg
      781 ctgtcgtcga acaacgtgct gcacaccgcg cacggcgagc cggtcatcgt gccctcgcag
      841 gacatcgtgc tgggcctgta ttacgccacc cgcgagcgcg tgaacgcgcg cggcgagggc
      901 tcgttcttcg tgaacatctc ggaagtgagc cgcgcgtacg agtcgcacgg cgtcgagctg
      961 cacgcgagga tcacggtgcg catcaaggag tcgcgcaaga acgacgacgg cacgctcacc
     1021 gaagtggtca accgctacga gacgacggtc ggccgcgcgc tcatgtcgga gatcctgccg
     1081 gcggggctgc ccttttcgat catcaacaag ccgctcaaga agaaggaaat ctccaagctc
     1141 atcaacgagg cgttccgccg ctgcggaatc cgcgagacgg tgatcttcgc cgacaagctg
     1201 atgtacacgg gattcagctt cgccacgcgc gcggggctgt cgatctgcgt cgacgacctg
     1261 ctgatcccgg acgagaaggc cggcatcatc gaggagagcg aaaagcaggt gaaggagatc
     1321 gagcgccagt acacctcggg cctcgtgacc cagggcgagc gctacaacaa ggtggtcgac
     1381 atctggggac gctcgggcga caaagtcgcc aaggcgatga tggaccagct cggcaaccag
     1441 aagaccaccg accgcaaggg caaggaggtc accgaggaat cgttcaactc catctacatg
     1501 atggccgact cgggcgcgcg cggctcggcg gcgcagatcc gccagctcgc gggcatgcgc
     1561 ggcctcatgg cgaagccgga cggctcgatc atcgagacgc cgatcaccgc caacttccgc
     1621 gagggcctga acgtcctgca gtacttcatc tcgacgcacg gcgctcgtaa gggcctggcg
     1681 gacaccgcgt tgaagacggc caactcgggc tacctgacgc gccgcctggt cgacgtcgcg
     1741 caggacgcca tcatcgccga gtacgactgc ggcaccatgg acggcatctc gctgggcgcg
     1801 ctggtcgaag gcggcgagat catcgaggcg atgagcgagc g
//
LOCUS       DASVII010000198         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_548978,
            whole genome shotgun sequence.
ACCESSION   DASVII010000198 DASVII010000000
VERSION     DASVII010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548978"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /gene="hemA"
                     /locus_tag="VF309_02535"
     CDS             <1..1227
                     /gene="hemA"
                     /locus_tag="VF309_02535"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7465489.1"
                     /translation="EVREKLAFPADRQGEALADLARQPGVAEAVLVSTCNRTEIYFRG
                     DESGAAGAWLEREAGKTGLSLADHLYVHTEEAAVRHAFRVASGLDSMVVGEPQILGQV
                     KQSVRNAESVGALGPTLGRLFQKTFAVAKQVRTETALGAQSISMAAAACRLAQNVFGD
                     LSRTKVCLIGVGEMVELAATYFAAQRPEALVVANRTLARGEEFAQRFAAKAIALAELP
                     SRLAEFDIIVTGPASTLPILGKGLIERALKARRRKPVFIVDFAVPRDVEAEVSTLEDV
                     FLYTIDDLGAIVQQGAESRRGAATEAEAIVDRHVVAFREWQDSRAAAPVIVELRRRAD
                     QYREMELAKAKGRLARGDDPAAVLESLAKGLANKFLHHPSQALSRAPVEERESLARAI
                     ETLYPDLEGRDPTSDP"
     gene            1224..>1878
                     /locus_tag="VF309_02540"
     CDS             1224..>1878
                     /locus_tag="VF309_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEX7465490.1"
                     /translation="MKASIAAKLASLDSRLREIDSRLSDPQVVDDLDNFRKLSQERSE
                     IEPVVALFNEHRRAEGDVAAAEDMARDPKMRAFAEDEIKSGRERLAALEADLQKMLLP
                     RDPNEGKNLFLEIRAGTGGDESALFAGDLLRMYARFAERNRWQVEIVSESLGEAGGYK
                     EVIARIVGDGAYSKLKFESGTHRVQRVPATEAQGRIHTSACTVAILPETDEIDSIEIN
                     "
BASE COUNT          298 a          649 c          667 g          264 t
ORIGIN      
        1 gaggtccgcg agaagctcgc cttccccgcg gaccggcagg gcgaagcgct cgcagacctt
       61 gcccgccagc ccggcgtggc cgaggcggtg ctcgtctcga cctgcaaccg caccgagatc
      121 tactttcgcg gcgacgagtc cggcgccgcg ggcgcgtggc tcgagcgcga agcggggaag
      181 acgggcctgt ccctcgcgga ccatctctac gtgcacaccg aagaggcggc cgtgcgccat
      241 gcattccggg tcgcctcggg cctcgattcg atggtcgtcg gcgagccgca gatcctcggc
      301 caggtgaaac aatcggtccg caacgccgag agcgtgggcg cgctcggccc caccctgggg
      361 cggctcttcc agaagacctt cgcggtcgcc aagcaggtgc gcacggagac cgccctcggc
      421 gcgcagtcga tctcgatggc cgcggccgcg tgccgcctcg cgcagaacgt gttcggcgat
      481 ctttcgcgca cgaaggtatg cctcatcggc gtcggcgaaa tggtggagct cgcggcgacc
      541 tatttcgcgg cgcagcgtcc cgaggcgctg gtcgtcgcga atcgcacgct cgcgcgcggc
      601 gaggagttcg cgcagcgctt cgcggcgaag gcgatcgcgc tggccgagct tccgtcgcgc
      661 ctcgcggagt tcgacatcat cgtcaccggc cccgcgtcga cgctgccgat tctcggcaag
      721 gggctcatcg agcgggcgct caaggcgcgg cgcaggaagc ccgtgttcat cgtcgacttc
      781 gccgtgccgc gcgacgtcga ggccgaggtg tcgacgctcg aggacgtgtt cctctacacg
      841 atcgacgatc tcggcgcgat cgtgcagcag ggcgccgaga gccggcgggg cgccgcgacc
      901 gaggccgagg cgatcgtcga ccggcacgtg gtggcgtttc gcgaatggca ggactcgcgc
      961 gccgcggcgc ccgtcatcgt cgagctgcgc cgccgcgccg accagtatcg ggagatggag
     1021 ctcgccaagg cgaaaggcag gctcgcgcgg ggcgacgatc ccgcggcggt gctcgagtcg
     1081 ctcgccaagg ggctggcgaa caagttcctg caccatccgt cgcaggcgct ttcgcgcgcg
     1141 cccgtggaag agcgcgaatc gctcgcgcgc gcgatcgaaa cgctctaccc ggatctcgaa
     1201 gggcgcgatc cgacttccga tccatgaagg cgtcgatagc ggcgaaactc gcgagcctcg
     1261 actcgcggct gcgcgagatc gactcgcgcc tgtcggatcc ccaggtcgtc gacgacctgg
     1321 acaatttccg caagctctct caggagcgct ccgagatcga gcccgtggtc gccctcttca
     1381 acgagcaccg cagggcggag ggcgacgtcg ccgccgccga ggacatggcg cgcgacccga
     1441 agatgcgcgc gttcgcggag gacgagatca agtcgggccg cgagcggctc gcggcgctcg
     1501 aggcggacct gcagaagatg ctcctgccgc gcgatcccaa cgagggcaag aacctcttcc
     1561 tcgagatccg cgccggcacc ggcggcgacg agagcgcgct cttcgcgggg gatctcctgc
     1621 gcatgtacgc gcgcttcgcg gagaggaacc gctggcaggt cgagatcgtc tccgagagcc
     1681 tcggcgaggc gggcggctac aaggaagtca tcgcgcggat cgtcggcgac ggcgcctatt
     1741 cgaagctcaa gttcgagtcg ggcacgcatc gcgtgcaacg cgtgccggcc acggaggcgc
     1801 agggccgcat ccacacctcc gcctgcaccg tggcgatcct gccggagacc gacgagatcg
     1861 acagtatcga gatcaacc
//
LOCUS       DASVII010000199         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_45529, whole
            genome shotgun sequence.
ACCESSION   DASVII010000199 DASVII010000000
VERSION     DASVII010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45529"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..663
                     /locus_tag="VF309_02545"
     CDS             190..663
                     /locus_tag="VF309_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7465491.1"
                     /translation="MQTITPFLWFNDNAEEAANLYLSIFKDGKIHNVARYGEAGPGPA
                     GSCMTIDFELRGQRFLGLNGGPHFKFTEAVSFVVSCETQPEIDEYWERLSAGGSKGQC
                     GWLKDKFGLSWQIVPRILGGLASSKEPKKAQSMMRAMMTMTKLDIAELQKAYAAG"
     gene            complement(674..>1604)
                     /locus_tag="VF309_02550"
     CDS             complement(674..>1604)
                     /locus_tag="VF309_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465492.1"
                     /translation="QMTTTIGGHCADAAVHDPRIRAVVGYVPFGGWSFLPAFCEGQGG
                     AASVNKPYLAISGTADTTAPLTQMKQALSLFQSSRYMVELLDGKHEFRPEDAGDLFTW
                     MVTFFNAYLDVQADPGAMGRLIRMQGVNGGRVDNLTVDVHIPFATDGDAQVRELYNSR
                     PNHFYVTADATEIQFILAGGAGDGWSLTGQGFKAWTSLPPNAFMTAVPVCRFELVPRG
                     RPWSTFYTSSASDCASLKAGRGWLYTGTPWYIQPVNAQLACPDGYIGVNRAYNQGAKR
                     NDSNHRFSTSDSTMHDMERVGWVYEATVMCSRP"
BASE COUNT          279 a          510 c          552 g          263 t
ORIGIN      
        1 gtggcgagct gcgcaaacag cagcgaggcc acggtgacga gcgcggtcag gaagcgcatc
       61 ttgcgaatca tggacccagt ataggggggc tttcggggcg atttcttgat gttcggcaag
      121 gatgcggtgt cgaatcgggg ccccgccatt cgactacgca ggcacatcca accgaggagc
      181 acgcgcgcca tgcagaccat caccccattc ctgtggttca acgacaacgc ggaagaggcg
      241 gccaatctct acctctccat cttcaaggac ggaaagatcc acaacgtcgc gcgctacggc
      301 gaggcgggtc cggggccggc cggatcctgc atgacgatcg acttcgagct ccgcgggcag
      361 cgcttcctcg gcctgaacgg cgggccgcac ttcaagttca ccgaggccgt ctcgttcgtc
      421 gtgtcctgcg agacccagcc cgagatcgac gagtattggg agaggctctc cgccggcgga
      481 tcgaagggcc aatgcggatg gctgaaggac aagttcggcc tgtcgtggca gatcgtgccg
      541 cgcatcctgg gcgggctcgc atcgtcgaag gagccgaaga aggcccagag catgatgcgg
      601 gcgatgatga cgatgacgaa gctcgacatc gccgagcttc agaaggcgta cgcggcgggt
      661 tgaaggcgtc ccgctagggc cgcgagcaca tcaccgtcgc ctcgtacacc cagcccacgc
      721 gctccatgtc gtgcatcgtg gaatcgctgg tgctgaagcg atggttcgag tcgttgcgtt
      781 tggcgccctg gttgtacgcg cggttcacgc cgatgtatcc atccgggcac gcgagctgcg
      841 cgttcaccgg ctggatgtac cacggcgttc ccgtgtacaa ccagccgcgg cccgccttca
      901 gcgacgcgca atcgctcgcg ctggacgtat agaacgtgct ccacgggcgc ccgcgcggca
      961 cgagctcgaa gcggcatacg ggcacggcgg tcatgaacgc gttcggggga agcgacgtcc
     1021 acgccttgaa gccctgcccg gtgagcgacc agccgtcgcc cgcgccgccc gcgaggatga
     1081 actggatctc ggtcgcgtcg gcggtcacat agaagtggtt gggccgcgag ttgtagagct
     1141 cgcgcacctg cgcgtcgccg tcggtggcga acgggatgtg cacgtcgacg gtgaggttgt
     1201 ccacgcgccc gccgttgacg ccttgcatgc ggatgagccg gcccatcgcg ccgggatcgg
     1261 cctgcacgtc gagataggcg ttgaagaacg tcaccatcca cgtgaagagg tcgcccgcat
     1321 cctcggggcg gaactcgtgc ttgccgtcga ggagctcgac catgtagcgc gacgactgga
     1381 agaggctcag cgcctgcttc atctgcgtga ggggcgcggt cgtgtccgcg gtgccggaga
     1441 tcgcgagata gggcttgttc acgctcgcgg cgccgccctg gccctcgcag aacgcgggca
     1501 ggaacgacca tccgccgaag ggcacgtatc ccaccacggc cctgatcctc gggtcgtgga
     1561 cggcggcatc ggcgcagtgg ccgccgatgg tcgtcgtcat ctgc
//
LOCUS       DASVII010000200         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_297203,
            whole genome shotgun sequence.
ACCESSION   DASVII010000200 DASVII010000000
VERSION     DASVII010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297203"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /gene="ubiG"
                     /locus_tag="VF309_02555"
     CDS             <1..388
                     /gene="ubiG"
                     /locus_tag="VF309_02555"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX7465493.1"
                     /translation="EAFAEESPGAFDVVTCMEMLEHVPEPPSTVRACAKLVKRGGLVV
                     FSTLNRNAKSFLLAIVGAEYVLQLLPKGTHEYARFIRPSELSRACRDAGLAVTDLTGM
                     TYNPFTKTYSLGRDVDVNYLLATRRD"
     gene            395..1081
                     /gene="gph"
                     /locus_tag="VF309_02560"
     CDS             395..1081
                     /gene="gph"
                     /locus_tag="VF309_02560"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966645.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX7465494.1"
                     /translation="MIDAVLFDLDGTLADTAPDLARTVNMMLAKRGLAPVTLEKVRPH
                     VSQGARGMIMSAFAIGAGHPDFPAMRDEFLDLYAGNLCIDSRLFPGMESLLAHLEDES
                     IAWGVVTNKFERFAKPLIEALGLGKRCAVVVGGDTCARAKPFPDPLLFAAASMGIEAT
                     RILYVGDDERDVQAARAARMPVVVAGYGYLGDGPVPALWGADAVMDSPAALEGWIREA
                     ARKRPAPVTQ"
     gene            1078..>1508
                     /locus_tag="VF309_02565"
     CDS             1078..>1508
                     /locus_tag="VF309_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465495.1"
                     /translation="MSAPSGARRRLLQALAAAPLAASWPALAAGPSANRLLVLVFLYG
                     GNDAYNTCVPYTDRLYYRVRPTIAVPRDTVLKITESHGFHPSLAALMPSWQARELAIV
                     QGLGYAAGTQQHFRDEDTAFTGCDGEEYSRDGWVTRALGSSA"
BASE COUNT          218 a          544 c          492 g          254 t
ORIGIN      
        1 ggaagcgttc gcggaggaat cgcccggcgc gttcgacgtc gtcacttgca tggagatgct
       61 cgagcacgtg cccgagccgc cgagcacggt gcgggcctgc gcgaagctcg tgaagcgcgg
      121 cggcctcgtc gtcttctcga ccctcaatcg caacgcgaag tcgttcctcc tggcgatcgt
      181 cggcgccgaa tacgtgctcc agctcctgcc gaaaggcacg cacgagtacg cgcgcttcat
      241 ccgcccctcg gagctctcgc gcgcatgccg cgacgcgggg ctcgccgtaa ccgacctcac
      301 cggcatgacc tacaatccgt tcacgaagac ttattcgctc ggacgcgacg tggacgtgaa
      361 ctatctgctg gcgactcgcc gtgattgact cgccgtgatc gatgccgtcc ttttcgatct
      421 cgacggcacg ctcgccgaca ccgcgcccga cctggcgcgc acggtgaaca tgatgctcgc
      481 caagcgcggc ctcgccccgg tgaccctgga gaaagtgcgc ccccacgttt cccagggcgc
      541 gcgcggcatg atcatgtcgg ccttcgcgat cggcgccggc catcccgatt ttcccgcgat
      601 gcgcgacgag ttcctcgacc tgtacgcggg gaatctctgc atcgattcgc gcctctttcc
      661 gggcatggag agccttctgg cccatctcga ggacgagtcg atcgcctggg gcgtggtcac
      721 caacaagttc gagcgcttcg cgaagcctct catcgaggcg ctgggcctcg gcaagcgctg
      781 cgccgtggtg gtgggcggcg atacgtgcgc gcgcgccaag ccctttcccg atccgctgct
      841 gttcgccgcc gcctcgatgg gcatcgaagc cacgcgcatc ctctacgtcg gcgacgacga
      901 gcgcgacgtg caagcggcgc gcgccgcgcg aatgccggtg gtggtcgcgg gctatggata
      961 cctgggcgac ggacccgtgc ccgccctctg gggggccgat gccgtcatgg actctccggc
     1021 cgcgctggaa ggctggattc gcgaggcggc gcgcaagcgc cccgcgcctg tgacgcaatg
     1081 agcgcgccgt cgggcgcgcg gcgccggctt ctgcaagctc tcgcggcggc cccgctcgcg
     1141 gcctcttggc cggcgcttgc cgccggtccg tccgcgaacc gcctgctggt actggtgttc
     1201 ctttacggcg gcaacgatgc ctacaacacg tgcgtgccgt acaccgaccg actctactat
     1261 cgcgtgcgac ccaccatcgc ggtgccccgc gacacggtgc tgaagatcac cgaaagccac
     1321 ggcttccatc cgtccctcgc ggccctcatg ccctcctggc aggcccgcga gctcgcgatc
     1381 gtccagggcc tgggctacgc cgcgggcacg cagcagcatt ttcgcgacga ggatacggcg
     1441 ttcaccggat gcgacggtga ggaatactcc cgtgacggat gggtcacgcg cgcgctcggc
     1501 tcgagcgc
//
LOCUS       DASVII010000201         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_153199,
            whole genome shotgun sequence.
ACCESSION   DASVII010000201 DASVII010000000
VERSION     DASVII010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153199"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF309_02570"
     CDS             <1..491
                     /locus_tag="VF309_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465496.1"
                     /translation="DGLDIPLLTQFNSTFREDHVARKPPESAYDGLIHFPYERAREAL
                     FALARSGAPDSHLGHVLRYQDPKRGGWAMPTIASMIRLLQKGFATRPYRSSDSMVFAA
                     VEGRGTLEVGGTKFDLAPHDVAVVPGWMPYTLQASEDWVVFSFSDRSAQEKLGFFRDE
                     RL"
     gene            532..906
                     /locus_tag="VF309_02575"
     CDS             532..906
                     /locus_tag="VF309_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7465497.1"
                     /translation="MNVSSRRFANAVVLRVEGRLDQDTCAAFTTDLMKSVDTAAAEGG
                     AVVLDLSGLEYISSAGLRCFMLASRQAKSQHAKIAVAALQPMVAEIFQISHFNLVFQV
                     FPTVREALGAVSAEAAAAFEKG"
     gene            910..1866
                     /locus_tag="VF309_02580"
     CDS             910..1866
                     /locus_tag="VF309_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7465498.1"
                     /translation="MRVRFWGTRGSIPVALTAADVRDKLAQALVQASGKVFNSYEEAH
                     AYASSELDFNLTHTFGGHTPCVEIEIPGDEYYACDLGSGARPFGVHILAKQARKPATI
                     NVFMSHVHWDHIMGFPLFGPAYVPGTKIRIHGCHDVLEQAFRKQHSAPCFPVEWEQLG
                     ATIEFVKLEPGEPFLINGMTVTPHLQLHGGDSYGYRFEYEGKVVVYTTDSEHKLEDRA
                     EAEKFAKFFHNADVVIFDAMYALAEAISVKADWGHSSNIVGVELCQMAHAKHLVLFHH
                     EPANNDATLEGLLKEARRFEELTRGDQALKVSAAYDGLEIDL"
     gene            1899..>3161
                     /locus_tag="VF309_02585"
     CDS             1899..>3161
                     /locus_tag="VF309_02585"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX7465499.1"
                     /translation="MLLAALSLFLLFAPAANTLRNVVFDGYQQLFPLERTTDPVAIVV
                     IDEEAIAHYGQWPWPRTRIAELITRISEAQPASIGLDLFFSEPDRFSPAAMADEMPIL
                     PTNLAKALREMPSNDEQLAKAIEGRNVVLGIGTTSDVDPRFRGPPRAAPVVTSGAREA
                     EMPNWPGYIGSLDVIDAAAAGHGLMNSGPQDQVVRVVPIVERIQGVIVPTLGVESVRV
                     AEGAGLRLDDAPGGLLLLRLAGVSMPLQRDGTAWIRYSRHDESRMVSAVEVLSGKLDP
                     ERLRQKVVLVGVTGLGVLDHKSTPLGEFVPGVEIHAQIVENLVNGVALVRPTFATWIE
                     GAVFILCGFVLIWFIPRLSALQGINLVVFLVLLLLCIGLIAFRHFHMLLDFAWPALGT
                     IAVFGSTVVGTLSEAERQRRVLRDQAAHM"
BASE COUNT          495 a         1084 c         1050 g          532 t
ORIGIN      
        1 tggacgggct cgacatcccg ttgctcacgc aattcaacag caccttccgc gaggaccacg
       61 tcgcccgcaa gccccccgaa tcggcatacg acggcctcat ccattttccc tacgagcgcg
      121 cccgcgaagc gctcttcgcc cttgcgcgaa gcggcgcgcc cgactcgcac ctcgggcacg
      181 tccttcgata ccaggacccc aagcgcggcg gctgggcgat gccgaccatc gcgagcatga
      241 tccggctcct gcaaaaggga ttcgcgacgc gcccctatcg ctcgagcgac agcatggtct
      301 tcgccgccgt ggaagggcgc ggaacgctcg aggtgggcgg gacgaaattc gacctcgcgc
      361 cccacgacgt ggccgtcgtt cccgggtgga tgccctacac attgcaggcg agcgaggact
      421 gggtggtttt ctcgttttcc gaccgctcgg cccaggagaa gctcggtttc ttccgcgacg
      481 agcgcctgtg acacaatagg cgcgcccttc cgcccgccgc taactccaac aatgaacgtc
      541 tcctcccgga ggttcgccaa cgccgtcgtt ctccgcgtgg agggccggct cgaccaggac
      601 acctgcgccg cgttcaccac cgacctcatg aagtccgtcg acacggccgc ggccgagggc
      661 ggcgcggtgg tgctggacct ttccgggctc gagtacatct ccagcgcggg cctgcgttgc
      721 ttcatgctcg cctcgcggca ggcgaagtcg cagcacgcga agatcgcggt ggcggcgctg
      781 cagccgatgg tcgcggagat cttccagatt tcccatttca acctcgtgtt ccaggtgttc
      841 ccgacggtgc gcgaggcgct gggcgcggtg tcggccgaag ccgccgccgc gttcgaaaag
      901 gggtagccgg tgcgcgtgcg cttctggggc acccgagggt cgatcccggt ggcgttgacc
      961 gcggcggacg tgcgcgacaa gctcgcccag gcgctggtgc aggcctcggg aaaggtcttc
     1021 aacagctacg aggaagcgca cgcctacgcc tcgagcgagc tcgacttcaa cctcacgcat
     1081 accttcggcg gccatacgcc gtgcgtcgag atcgagatcc cgggcgacga gtattacgcg
     1141 tgcgacctgg gcagcggcgc gcggccgttc ggcgtgcaca tcctcgccaa gcaggcgcgc
     1201 aagcccgcga ccatcaacgt cttcatgtcc cacgtgcact gggaccacat catgggattc
     1261 ccgctcttcg ggccggcgta cgtgccgggg acgaagatcc gtatccacgg ctgccacgac
     1321 gtgctcgagc aggccttccg caagcagcat tccgcgccgt gcttcccggt cgagtgggag
     1381 cagctgggcg ccacgatcga gttcgtgaag ctcgagccgg gagagccgtt cctgattaac
     1441 ggcatgacgg tcacgccgca cctgcagctg cacggcggcg actcgtacgg ctatcgcttc
     1501 gagtacgagg gcaaggtcgt ggtgtacacg accgattcgg agcacaagct cgaggaccgc
     1561 gccgaggccg agaaattcgc gaagttcttc cacaacgccg acgtggtgat cttcgacgcg
     1621 atgtacgcgc tcgccgaggc gatcagcgtg aaggccgact ggggtcactc gagcaacatc
     1681 gtgggcgtgg agctgtgcca gatggcgcat gccaagcacc tcgtgctctt ccaccacgag
     1741 ccggcgaaca acgacgccac cctcgagggc ctgctcaagg aggcgcgccg cttcgaggag
     1801 ctgactcgcg gcgaccaggc gctcaaggtg tccgccgcgt acgacggcct ggagatcgat
     1861 ctctgagcac gctgcgaggc ctgcgggttc tcgccctggt gctcctcgcc gcgctgtcgc
     1921 ttttcctgct cttcgctccc gccgccaaca cgctgcgcaa cgtcgtcttc gacggctatc
     1981 agcagctctt cccgctggag cgcaccacgg accccgtcgc catcgtcgtg atcgacgagg
     2041 aggcgatcgc gcactacggg cagtggccct ggccccggac gcgcatcgcc gagctcatca
     2101 cccgcatctc ggaggcgcag cccgcgtcga tcggcctgga tctcttcttc tccgagcccg
     2161 accgcttctc gcccgcggcg atggccgacg agatgccgat cttgcccacg aacctcgcca
     2221 aggcgctgcg cgagatgccg tccaacgacg agcagctcgc gaaagcgatc gaaggtcgca
     2281 atgtagtgct cggcatcggc accacgagcg acgtcgaccc gcgctttcgc gggccgccgc
     2341 gcgcggcccc ggtggtcacg tcgggcgcgc gcgaagcgga gatgccgaac tggccgggct
     2401 acatcggcag cctcgacgtg atcgacgcgg cggcggcggg acacgggttg atgaactccg
     2461 gcccgcagga ccaggtggtg cgcgtggtgc cgatagtcga gcggatccag ggcgtgatcg
     2521 tgcccacgct cggcgtcgag tcggtgcgcg tcgcggaggg agcgggcctg cgcctggacg
     2581 atgcgcccgg aggcttgctc ttgcttcgcc ttgcgggcgt gagcatgccg ctccagaggg
     2641 acggcaccgc gtggattcga tacagcaggc acgacgagag ccgcatggtc tccgctgtcg
     2701 aggtgctgag cggcaagctc gatcccgaac gcctgcgcca gaaggtggtg ctggtgggcg
     2761 tgacgggcct gggcgtgctc gaccacaaat cgacgccgct cggcgagttc gtccccggcg
     2821 tcgagatcca tgcccagatc gtggagaacc tcgtgaacgg cgtggccctg gtgcgtccga
     2881 cattcgcgac gtggatcgag ggcgcggtgt tcatcctgtg cggattcgtc ctcatctggt
     2941 tcattccgcg gctctcggcg ctgcagggca tcaatctcgt ggtgttcctg gtgctgctcc
     3001 tgctgtgcat cggcctcatc gccttccggc atttccacat gctcctcgat ttcgcctggc
     3061 ccgcgctggg cacgatcgcc gttttcggca gcaccgtggt cggcacgctt tcggaagccg
     3121 agcgccagcg ccgggtgttg cgcgaccagg cggcgcacat g
//
LOCUS       DASVII010000202         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_620773,
            whole genome shotgun sequence.
ACCESSION   DASVII010000202 DASVII010000000
VERSION     DASVII010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620773"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..811
                     /locus_tag="VF309_02590"
     CDS             191..811
                     /locus_tag="VF309_02590"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX7465500.1"
                     /translation="MKKTLLAGALLSSALVPGVVLAQAYGDTARVISATPLYDRVAEP
                     RRECHDEQVTAYEERRGVRPASEARESGGVGAGTVIGAIVGGVIGHQFGGSSAGRDHG
                     TAAGAIVGGLVGNQVDRDNDANATRDVVVERVPVTRNVQRCSEIADTRDVVIGYDVRY
                     EYNGHELRARLPYDPGPQMPVNVDVRPPAPPAPIADPRMPRYRGTY"
     gene            868..1461
                     /locus_tag="VF309_02595"
     CDS             868..1461
                     /locus_tag="VF309_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX7465501.1"
                     /translation="MKKTLVAVAVASAFVSLGAFAQSFTDSAPVIGVHAITERIPVNR
                     EECWSEQRRGYEERTVTRQDNGAQIGAGTVIGAIAGGVLGHQMGSSSGGRDRGTAAGA
                     IVGGLVGNQIDRSNNAGPDTVEVERRPVNREVERCRTIQEVREVASGYDVRYRYGNRE
                     FVTRMPFDPGRRIRIAVDIRPVVEPDRGGPGAPEYRR"
BASE COUNT          265 a          561 c          542 g          224 t
ORIGIN      
        1 gaagcgaact acatggacga aatatctcct tacacattcc gcgaggccaa ggccggcgcg
       61 atccgcccga tccttcgcga gcagctcgag atcgcgacgc gctgggcgat ggagcggccg
      121 gggcgtcaac ggggggccgc atcgcgcgtt taacccatca ggacccgttg tccccatggg
      181 agaagaacgc atgaagaaga ccctcctcgc cggcgcgtta ttgtcgtcgg ccctcgtgcc
      241 gggcgtcgtg ctcgcgcaag cttacggcga caccgcgcga gttatttccg cgacacccct
      301 ctacgaccgc gtcgccgaac cgcgacgcga atgccatgac gagcaggtga ccgcctacga
      361 agagcgccgc ggcgttcgcc ccgcgtccga ggcgcgcgaa tcgggcggcg tcggcgcggg
      421 caccgtgatc ggcgcgatcg tgggcggcgt gatcggccac cagttcggcg gcagcagcgc
      481 cgggcgcgac cacggcacgg ccgcgggcgc catcgtgggc ggcctcgtcg gcaaccaggt
      541 cgatcgcgac aacgacgcga atgcgacccg cgacgtcgtc gtcgagcgcg tgccggtcac
      601 gcgcaacgtg cagcgctgca gcgagatcgc cgacacgcgc gacgtcgtga tcggatacga
      661 cgttcgctat gagtacaatg gacatgaatt gcgcgcgagg ctgccctacg acccgggccc
      721 gcagatgccg gtgaacgtgg acgtgcgccc gcccgccccg cccgcgccga tcgcggatcc
      781 gcgcatgccc cgctaccgcg gcacctattg aactttcgcg atgccccgcg gccccagaac
      841 aatccactac ccctcgagag gttgcacatg aagaagaccc ttgtcgcagt cgccgtcgct
      901 tcggcgttcg tttcgctggg cgcgttcgcc cagtctttca ccgactccgc tccggtgatc
      961 ggcgtgcacg cgatcaccga gcgcattccc gtcaatcgcg aggagtgctg gagcgagcaa
     1021 cgccgcggct atgaagaacg caccgtcact cgccaggaca acggcgcgca gatcggggcc
     1081 ggcaccgtca tcggcgcgat cgccggcggc gtgctgggcc accagatggg atcgagcagc
     1141 ggtggacgcg atcgcggcac cgcggcgggc gccatcgtcg gcggcctcgt cggcaaccag
     1201 atcgaccgca gcaacaacgc gggccccgac accgtcgagg tcgagcgccg ccccgtgaat
     1261 cgcgaggtgg agcgttgcag gaccatccag gaagtgcgcg aggtcgcctc cggctacgac
     1321 gtgcgctacc gctacggcaa ccgcgagttc gtcacgcgca tgcccttcga tccgggccgg
     1381 cgcattcgca tcgcggtcga catccgcccg gtggtggaac cggatcgcgg cggcccgggc
     1441 gccccggagt accgtcgcta ggacgcgaca tccggcgaac gaacggcgcc gcgaggcgcc
     1501 gttttctttt caacatcccg gtgatcggcc gggcgtcgaa ggggcggttc ttctcgaccc
     1561 gggggacctc ggcctctcga cggggagaga tc
//
LOCUS       DASVII010000203         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_549197,
            whole genome shotgun sequence.
ACCESSION   DASVII010000203 DASVII010000000
VERSION     DASVII010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549197"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..728)
                     /locus_tag="VF309_02600"
     CDS             complement(114..728)
                     /locus_tag="VF309_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX7465502.1"
                     /translation="MTDPDTIIACRECDLLQREVTPPLHGEAQCARCGAELFRNKPRS
                     LEHTLAFLFAAAVFFVIGNSFPVMSLDAQGVRTTTTLFGASHALFLSGDQALAVLVFM
                     TTILFPGLEIAAMLYLLLGLKGARLPRAFAPTFRLVEAIKPWGMISVFVLGTLVSLVK
                     LHNIATVIPGLALYALGALILMLTASEAAFEPRELWARARAVRA"
     gene            771..>1527
                     /locus_tag="VF309_02605"
     CDS             771..>1527
                     /locus_tag="VF309_02605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX7465503.1"
                     /translation="MEATTFDPGIGREFIDRAQSLARHSDSPAHYTRTYLTPAHRAAS
                     RQIAEWMRAAGLAVRLDAVGSVIGRYEAAAPGAKTLLMGSHFDSVRNGGKYDGVLGIL
                     VPIACVGELQRRGERLAVAIEIAAFADEEGARFQTSFLASRAMIEGSASLSPLLDRRD
                     ADGVVLREAMAGAGLEPARADADRIDPESLAAYVEVHIEQGPVLLNGGHALGVVTSIA
                     AGTRHLLRVKGEAGHAGTVPMAMRRDAMTAAAEM"
BASE COUNT          251 a          510 c          563 g          203 t
ORIGIN      
        1 gtgcagcgcg gcgccgcagc gcgcgcatcc catgcgcgaa aggccgccgc acgggcgctg
       61 gatctgcgca caggcatggc agaccagcag ccccgagcgc gcggcggtgg ccgtcatgcc
      121 cgcacggcgc gcgcgcgcgc ccagagctcg cgcggctcga acgccgcctc gctcgcggtg
      181 agcatgagga tcaacgcgcc cagcgcatag agcgcgaggc ccgggatcac ggtggcgatg
      241 ttgtgcagct tcaccagcga caccagcgtg cccagcacga agaccgagat catgccccag
      301 ggcttgatcg cctcgaccag gcggaaggtg ggcgcgaagg cccgcggcaa ccgggcgccc
      361 ttgaggccca ggaggagata gagcatcgcg gcgatctcca gcccgggaaa gaggatggtg
      421 gtcatgaaca ccagcacggc gagcgcctgg tcgccgctca ggaaaagcgc gtgcgaagcg
      481 ccgaacaggg tggtcgtggt gcgcacgccc tgcgcgtcga gcgacatcac cggaaacgag
      541 ttgccgatca cgaagaagac cgccgcggcg aagaggaagg cgagcgtatg ttcgaggctc
      601 ctcggcttgt tgcggaagag ctccgccccg caacgcgcgc actgggcctc gccatgcagc
      661 gggggcgtga cctcgcgctg caggaggtcg cattcacggc aggcgatgat ggtgtccggg
      721 tctgtcattg ttctgggcta cactccaccg cgctctcgaa gactgcatgc atggaagcca
      781 cgacattcga tcccggcatc ggccgggagt tcatcgaccg ggcgcaatcc cttgcccggc
      841 acagcgactc gcccgcgcat tacacacgaa cttaccttac tccggcgcat cgcgcggcgt
      901 cgcggcagat cgccgaatgg atgcgcgcgg cgggcctcgc ggtgcgattg gacgccgtgg
      961 ggagcgtgat cggccgctac gaggccgccg cccccggggc gaagacgctc ctcatgggct
     1021 cgcacttcga ttcggtgcgc aatggcggca agtatgacgg agtgctggga atcctcgtgc
     1081 cgatcgcgtg cgtgggcgag ctccagcggc gcggcgagcg cctggcggtg gcgatcgaga
     1141 tcgccgcctt cgccgacgag gagggcgcgc gcttccagac gagcttcctc gcgagccgcg
     1201 cgatgatcga aggctcggca tcgctctcgc ctctcctcga ccgccgcgat gccgacggcg
     1261 tcgtgctgcg cgaagcgatg gccggggcgg ggctcgaacc cgcgcgcgcc gacgcggatc
     1321 gcatcgatcc ggagagcctg gcggcgtatg tcgaggtcca tatcgagcag ggcccggtgc
     1381 tgctgaatgg cgggcacgcg ctgggcgttg tgaccagcat cgccgccggc acgcgccatc
     1441 tgctgcgggt gaagggcgag gccggccacg cgggcacggt gccgatggcg atgcgccgcg
     1501 acgcgatgac cgcggcggcc gagatgg
//
LOCUS       DASVII010000204         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_45770, whole
            genome shotgun sequence.
ACCESSION   DASVII010000204 DASVII010000000
VERSION     DASVII010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45770"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF309_02610"
     CDS             complement(<1..326)
                     /locus_tag="VF309_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7465504.1"
                     /translation="MARTPLLRAFQRLAEEHRAAESLGIPPAELRGRRQESAYTRRDF
                     LKRAGVTGAAAATVGPLAFAQRAGANSSPTAPRIAIVGGGIAGLSAALKLADKGVAST
                     VYEAST"
     gene            430..948
                     /locus_tag="VF309_02615"
     CDS             430..948
                     /locus_tag="VF309_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7465505.1"
                     /translation="MRSSEGQPLRLPARAEASRGPVAPARKVDDLDRGIIEELQKNGR
                     ESFRRIAARLGVSEATVRARYARLTSEGILQVVAVTNPLGLGFEQALVGIKTSGPPEN
                     VADELARWPQVDYVVVTAGQFDVIVEVVAADRRELLDLTNKMRALDGVVTTEIFFYLE
                     MWKQLYDWGTRE"
     gene            945..>2163
                     /locus_tag="VF309_02620"
     CDS             945..>2163
                     /locus_tag="VF309_02620"
                     /inference="COORDINATES: protein motif:HMM:NF005102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7465506.1"
                     /translation="MSTTSTAMSIEQLQQAARDHLWLHFTRMGGQDAPIIMRGEGCYL
                     EDSNGKRYLDALAGLFAVQVGYSYGEEMGQAALDQMRELPFYTNWSYAHPRAIELAHE
                     IAQLTPGDLNRVFFVSGGSEAVESAWKLARQYHQARGERRWKAVGRRLAYHGTTMGAL
                     SINGIAAIRAPFEPLVPDTIHVRNTNRYHRPEHETEEEFTAFLLEDLDSAIAQAGPET
                     VAMVLMEPVQNAGGSFTPPAGYWRGVREICDRYGVLLCADEVITGFGRLGSWFGSEHY
                     DIKPDLITCAKGLSSAYASIGAVVASDRVVEPFLDDKSTYAHGITFGGHPVQCAIALK
                     NLEIMKRERIVENVRDNGDAFHATLAQLLDLPIVGDLRGTGFFYALELVKDKETRETF
                     NAEECETLLRGFLS"
BASE COUNT          350 a          718 c          744 g          351 t
ORIGIN      
        1 tccgtcgacg cttcgtagac cgtggacgcg acgcccttgt ccgcgagctt gagcgcagcg
       61 ctcagccccg cgatgccgcc gccgacgatc gcgatgcgcg gcgcggtggg cgagctgttc
      121 gcgccggcgc gctgcgcgaa cgcgagcggc ccgacggtcg cggccgccgc gccggtgacg
      181 ccggcgcgct tgaggaagtc gcggcgcgtg taagcacttt cctgcctccg cccgcggagc
      241 tcggcgggcg ggatgcccag gctttcggct gcgcggtgtt cttcggcgag ccgctggaac
      301 gcgcgaagca ggggtgtgcg tgccacggtg tgaccctcct cggataggtg gcgggacgat
      361 ctcgcatctc ggcggcagaa gtcaagtcga ttgacgcaat gcgcagcaga gtggtacgaa
      421 tgccacgcgt tgcgcagttc cgagggccaa ccgcttcgtt tacccgcccg cgcggaagcc
      481 tcgcgtggac ccgtcgcccc tgcccggaag gtcgacgatc tcgaccgcgg gatcatcgag
      541 gagctgcaga agaacggccg cgagtcgttc cgccgcatcg ccgcgcggct cggcgtgtcg
      601 gaagcgacgg tgcgcgcccg ttatgcgcgc ctgacgagcg aggggattct ccaggtggtc
      661 gcggtcacga atccgctcgg gctcggtttc gagcaggctc tcgtcggcat caagacgtcc
      721 ggcccgccgg agaacgtcgc cgacgagctc gcccgctggc cgcaggtcga ctacgtcgtc
      781 gtgaccgcgg gacagttcga cgtcatcgtc gaggtcgtcg ctgcggaccg gcgcgagctt
      841 ctggatctca cgaacaagat gcgcgcgctc gacggtgtcg tgacgaccga gatcttcttc
      901 tatctcgaaa tgtggaaaca gctctacgac tgggggacgc gcgaatgagc accacgagca
      961 ccgccatgag catcgagcag ctgcagcagg ctgcgcgcga ccacctctgg ctgcatttca
     1021 cgcgcatggg cggtcaggat gcgccgatca tcatgcgggg ggagggttgc tacctcgagg
     1081 actcgaacgg aaagcgatat ctcgatgcgc tcgcgggcct cttcgcggtg caggtcggct
     1141 attcgtacgg cgaggagatg gggcaggccg cgctcgacca gatgcgggag ctgcccttct
     1201 acacgaactg gtcgtacgcc catccgcgcg caatcgagct cgcgcacgag atcgcccagc
     1261 tgacgccggg cgacctcaac cgcgtcttct tcgtctccgg cggctcggaa gcggtcgagt
     1321 cggcctggaa gctcgcgcgc cagtaccacc aggcgcgcgg cgagcggcgc tggaaggccg
     1381 tggggcgtcg cctcgcctac cacgggacga ctatgggcgc gctctcgatc aacggcatcg
     1441 ccgccatccg cgcgccgttc gagccgctcg tccccgacac gattcacgtg cgcaacacca
     1501 accgctacca ccgaccggag cacgagaccg aggaggagtt cacggcgttc ctgctcgagg
     1561 atctcgactc ggcgatcgcg caggcgggtc ctgagactgt ggcaatggtc ttgatggagc
     1621 ctgtgcagaa cgccggcggc tcattcaccc cgcccgcggg gtactggcga ggcgttcgcg
     1681 agatctgcga ccgctacggc gtgctgctct gcgccgacga agtgatcacc ggcttcgggc
     1741 ggctcggctc ctggttcggc tcggagcact acgacatcaa gccggacctg atcacctgcg
     1801 cgaaggggct ttcgtccgca tacgcgtcga tcggtgcggt cgtcgcatcg gaccgcgtcg
     1861 tcgaaccgtt cctcgacgac aagtcgacgt acgcgcacgg gatcacattc ggcggacatc
     1921 ccgttcagtg tgcgatcgcg ctcaagaacc tcgagatcat gaaacgcgag cgcatcgtcg
     1981 agaacgtgcg ggacaacggt gacgcgttcc acgcgaccct ggcgcagttg ctcgacctgc
     2041 cgatcgtcgg cgacctgcgc gggacagggt tcttctacgc gctcgagctc gtgaaagaca
     2101 aggagacgcg cgagacgttc aacgccgagg agtgcgagac gctgcttcgc ggctttctct
     2161 cgc
//
LOCUS       DASVII010000205         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_153471,
            whole genome shotgun sequence.
ACCESSION   DASVII010000205 DASVII010000000
VERSION     DASVII010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153471"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF309_02625"
     CDS             <1..300
                     /locus_tag="VF309_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7465507.1"
                     /translation="ATPGGIVSDPETLELLLAHCYTVWLQASAEEHMQRVVAQGDLRP
                     MAASTEAMEDLRRILAGRTPFYAKADAAVDTSGAALDASFAALRSQARSALQLPE"
     gene            306..1973
                     /gene="boxC"
                     /locus_tag="VF309_02630"
     CDS             306..1973
                     /gene="boxC"
                     /locus_tag="VF309_02630"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEX7465508.1"
                     /translation="MNDSSAAPGRVDYRTEPSRYRHWKLSYDGAVATLAMDVAEDGGI
                     RPGYKLKLNSYDLGVDIELADALNRIRFEHPEVRTVVVTSAKERIFCSGANIFMLGTS
                     SHAWKVNFCKFTNETRNGIEDSSRNSGLRFVAAVNGTCAGGGYELALACDEIVLVDDR
                     SSAVSLPEVPLLGVLPGTGGLTRLTDKRRVRHDLADVFCTTAEGVRGQRALDWKLVDA
                     IVKPGQFARAVEDRAQAIAAASARAAAKPVALTPLAKEYEGDLLDYEFVKVAIDRARR
                     VARITVTAPAAGQPRDLAGIEAAGAHWWPLAMARELDDAILTLRTNELDIGTWILATA
                     GDSEAVLACDAALRAHADNGFVREVTGMLRRALARLDVSSRTLFALIEPGSCFAGTLA
                     ELAFAADRTYMLALPGEPQRAPAITLSEMNFGAYPMVTGRARLARLFHDDAGELGAAR
                     AAIGRPLDAKAASALGLVTAAPDDIDWDGEVRIALEERAAMSPDALTGLEANLRFGGT
                     ETLETRIFGRLSAWQNWIFSRPNAVGDKGALKVYGKGEKAAFDWTRV"
BASE COUNT          334 a          747 c          750 g          301 t
ORIGIN      
        1 gcgacaccgg gcggcatcgt ctcggacccg gagacgctcg agctccttct cgcacattgc
       61 tacacggtgt ggctgcaggc ctctgccgag gagcacatgc agcgcgtcgt ggcgcaaggc
      121 gacctgcggc cgatggcggc gagcaccgag gcgatggaag acctgcgccg catcctcgcg
      181 ggacgcacgc ccttctacgc caaagcagac gccgccgtgg acaccagcgg cgcggcgctc
      241 gatgcgagct tcgccgcgct gcgctcgcag gcgcgctccg ccctgcagct tccggagtga
      301 ccgacatgaa cgactcgagc gccgcacccg gccgcgtcga ctatcgcacc gagccctccc
      361 gctaccggca ctggaagctc tcctacgacg gcgcggtcgc gacgctcgcg atggatgtcg
      421 ccgaagacgg cggcatccgt cccggctaca agctgaagct gaattcatac gacctcggcg
      481 tcgacatcga gcttgccgac gcgctgaacc gtatccgctt cgagcacccc gaggtgcgca
      541 cggtggtcgt gacgagcgcg aaggagcgca tcttctgctc gggcgccaac atcttcatgc
      601 tcgggacatc gagccacgcg tggaaggtga acttctgcaa gttcaccaac gagacccgca
      661 atggcatcga ggactcttcg cgaaactcgg gactgcgctt cgtcgccgcg gtgaacggaa
      721 cctgcgcggg cggcggctac gagctggcgc tcgcgtgcga cgagatcgtg ctcgtggacg
      781 accgctcttc ggccgtgtcg ttgcccgagg tgccgctgct gggcgtgctt ccgggcacgg
      841 gcggactcac gcgcctcacg gacaagcgcc gcgtgcgcca cgatctcgcc gacgtcttct
      901 gcaccaccgc cgaaggcgtg cgcgggcagc gggcgctcga ctggaagctg gtggatgcga
      961 tcgtgaagcc ggggcagttc gcgcgggcgg tcgaggatcg ggcgcaggcg atcgcggccg
     1021 caagcgcgcg cgcggcagcc aagcccgtgg cgctcacgcc gctcgcgaag gaatacgagg
     1081 gcgacctcct cgactacgaa ttcgtgaagg tcgcgatcga ccgcgcgcgc cgcgtggcgc
     1141 gaatcacggt gacggcgcct gcggccgggc aaccgcgcga cctcgcgggc atcgaggcgg
     1201 cgggcgcgca ctggtggccg ctcgcgatgg cgcgagagct cgacgacgcg atcctcacgc
     1261 tgcgcaccaa cgagctcgac atcggcacct ggatcctcgc gaccgcgggc gattccgagg
     1321 cggtgctggc ctgcgacgcc gcgctgcggg cccatgcgga caacggcttc gtgcgcgagg
     1381 tgaccggcat gctgcgccgc gcgctcgcgc gcctcgacgt gtcgtcgcgc accctcttcg
     1441 cgttgatcga gccgggctcg tgcttcgcgg gcacgctcgc cgagctcgcc ttcgccgcgg
     1501 accgcaccta catgctggcg cttcccggcg agccgcaacg cgccccggcg atcactctct
     1561 cggagatgaa tttcggcgcg tatccgatgg tgaccgggcg cgctcgcctg gcgcggcttt
     1621 tccacgacga cgccggggag cttggcgcgg cgcgcgcggc catcggacgt cctctcgacg
     1681 cgaaggcggc aagcgcgctc ggcctcgtca ccgccgcgcc cgacgacatc gactgggacg
     1741 gcgaggtgag gatcgccctc gaggagcgcg ccgcgatgtc acccgacgcg cttaccgggc
     1801 tggaggcgaa cctgcgcttc ggcggcacgg agaccctgga gacgcgcatc ttcgggcgcc
     1861 tcagcgcgtg gcagaactgg attttctcgc gcccgaacgc ggtgggcgac aagggcgccc
     1921 tcaaggtgta cggcaagggc gagaaggccg ctttcgattg gacccgggta tgagcgccat
     1981 cgactacgca cagaaaattc ccaacaacgt ggacctcgcg ggcgaccgca cgctgcagcg
     2041 cgcgctcgag caatggcatc ccggctacac gcagtggtgg aaggagatgg ggcccgaggc
     2101 cagcgacggg ttcgacgtct acctgcgcac gg
//
LOCUS       DASVII010000206         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_10148, whole
            genome shotgun sequence.
ACCESSION   DASVII010000206 DASVII010000000
VERSION     DASVII010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10148"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VF309_02635"
     CDS             <1..776
                     /locus_tag="VF309_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7465509.1"
                     /translation="GTEKDTVEQLRIGGLDMMRINAAALNSIAPETIVTVMPFLFRSP
                     EHMHKVLDGPIGDEILASLGSEGIVGLAFYDSGARSFYTAKKPIRTLADMKGLKVRVP
                     QSDLFVALIEALHANPTPMPYGEVYTALKTGIVDAAENNWPSYESSRHFESARFYTLS
                     EHSMAPEVLAFSRIVWDKLSKEDQALIRKAAKDSVPYMRKLWDEREMKSRKVVEAAGT
                     QVITIANKQEFIDAMKPVYAKFADTPRLKDLVKRIQDTK"
     gene            776..1270
                     /locus_tag="VF309_02640"
     CDS             776..1270
                     /locus_tag="VF309_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7465510.1"
                     /translation="MRLLTRVNARIARACMYFSVTGLFVIVAIVAFQVFGRYVMNDSP
                     TWTENLALVLILYVTLLGAAVGVRDAGHIGMESLLVLVPEAIRNKLEIVIHILVAIFG
                     GYMVYNGWVLGASVWAYKLPNINLSEGVRYVPLIISGTLIVLFCIEHVIAAVRGEEVK
                     PSWH"
     gene            1261..2550
                     /locus_tag="VF309_02645"
     CDS             1261..2550
                     /locus_tag="VF309_02645"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7465511.1"
                     /translation="MALTILAVTFTLLLLLGVPVAFAIGLSSVCTILYEGLPVAVLFQ
                     QMMSGMNIFSFLAIPFFVFSGELMLHGGIADKIVTLARNLVGHVRGGLGMANVLACTL
                     FGGVSGSPVADVSAMGAVMIPMMKREGYGTDYAVNVTTHASLVGALMPTSHNMIIYSL
                     AAGGTVSIGALIAAGVVPAIVLCLCMLVAAYLVAVKRGYPSGVFPGWNQVWRSLAAAI
                     PGLLVVVIILAGILSGVFTATESASIAVVYSITLTFFIYRTMTWENFLKAAARAVKTT
                     GIVLLLIGVSAMFQYLMALYSVAELAGGWLSAVSSNPWTIFFLINLILFILGTFMDMA
                     ATILICTPIFLPIAMKFGMDPVQFGMVMLINCALGLNTPPVGTTQFVGCAIGGISVGE
                     VMRTILPFYSALLAALFLVTYIPGFSMWLPHLLLGTR"
BASE COUNT          421 a          873 c          843 g          498 t
ORIGIN      
        1 tcggcaccga gaaggacacg gtcgagcagc tgcgcatcgg cggcctggac atgatgcgca
       61 tcaacgccgc ggcgctgaac agcatcgccc ccgagaccat cgtcacggtg atgccgttcc
      121 tgttccgctc gcccgagcac atgcacaagg tgctcgacgg ccccatcggc gacgagatcc
      181 tcgcctccct ggggtccgag ggcatcgtgg gcctcgcctt ctacgacagc ggcgcacgct
      241 cgttctacac ggccaagaag ccgatccgca cgctcgccga catgaagggg ctcaaggttc
      301 gcgtgccgca atcggacctg ttcgtggccc tcatcgaggc gctgcacgcg aatcccacgc
      361 cgatgccgta cggcgaggtc tatacggcgc tgaagaccgg gatcgtggac gccgcggaga
      421 acaactggcc gtcctacgaa tcgtcgcgcc acttcgagtc ggcccggttc tacacgctga
      481 gcgagcactc gatggcgccg gaagtcctcg cgttctccag gatcgtctgg gacaagctct
      541 cgaaggagga ccaggcgctg atccgcaagg ccgccaagga ctcggtgccc tacatgcgca
      601 agctctggga cgagcgcgag atgaagtcgc gcaaggtcgt cgaggccgcg ggcacgcagg
      661 tgatcaccat cgcgaacaag caggagttca tcgacgcgat gaagccggtg tacgcgaagt
      721 tcgccgacac gccgcggctg aaggacctgg tgaagcgcat ccaggacacg aagtagtgcg
      781 gctcctgacg cgagtgaacg cgcgcatcgc gcgcgcgtgc atgtattttt ccgtgacggg
      841 gctcttcgtg atcgtcgcga tcgtcgcctt ccaggtgttc ggccgctacg tgatgaacga
      901 cagccccacc tggacggaga acctcgcgct ggtgctgatc ctctacgtga cgctcctcgg
      961 cgccgccgtg ggcgtgcgcg acgcgggcca catcggcatg gaatcgctgc tggtgctggt
     1021 ccccgaggcg atccgcaaca agctcgagat cgtgatccac atcctcgtgg ccatcttcgg
     1081 cggatacatg gtctacaacg gctgggtgct gggcgcctcg gtctgggcgt acaagctgcc
     1141 caacatcaat ctctcggaag gcgtgcgcta cgtgccgctg ataatctcgg gcacgctcat
     1201 cgtcctcttc tgcatcgagc acgtgatcgc cgcggtgcgc ggcgaggaag tgaagccctc
     1261 gtggcactga cgatcctcgc ggtcaccttc accctcctgc tgctgctggg cgtcccggtg
     1321 gcgttcgcga tcggcctgtc ctcggtgtgc accatcctct acgagggcct gccggtcgcg
     1381 gtgctcttcc agcagatgat gtccgggatg aacatcttct cgttcctggc gatcccgttc
     1441 ttcgtcttca gcggcgagct gatgctgcac ggcggcatcg ccgacaagat cgtcacgctc
     1501 gcgagaaacc tggtgggcca cgtgcgcggc ggcctgggca tggcgaacgt gctcgcgtgc
     1561 acgctcttcg gcggagtctc gggctcgccc gtcgccgacg tctcggccat gggcgcggtg
     1621 atgatcccga tgatgaagcg cgagggctac ggcaccgact acgccgtgaa cgtgaccacg
     1681 cacgcctcgc tcgtcggcgc gttgatgccc accagccaca acatgatcat ctactcgctc
     1741 gccgcgggcg ggacggtgtc gatcggcgcg ctcatcgcgg cgggcgtggt gcccgcgatc
     1801 gtgctgtgtc tttgcatgct ggtggccgcc tacctggtgg cggtgaagcg cggctacccg
     1861 tcgggcgtct ttcccggatg gaaccaggtc tggcgatcgc tcgccgcggc catcccgggc
     1921 ctgctggtgg tcgtcatcat cctcgcgggg atcctctcgg gcgtcttcac ggcgaccgaa
     1981 tcggcgtcga tagccgtcgt gtactcgatc acgctcacgt tcttcatcta ccgcaccatg
     2041 acctgggaga acttcctcaa ggcggcggcg cgggcggtga agaccaccgg catcgtgctg
     2101 ctgctgatcg gcgtctcggc gatgttccag tacctgatgg cgctctattc ggtcgcggag
     2161 ctggcgggcg gctggctctc ggcggtgtcc tcgaacccgt ggacgatctt cttcctgatc
     2221 aacctcatcc tcttcatcct cggcaccttc atggacatgg cggccaccat cctcatctgc
     2281 acgccgatat tcctgccgat cgcgatgaaa ttcggcatgg acccggtgca gttcggcatg
     2341 gtgatgctga tcaactgcgc gctcggcttg aatacgccgc ccgtgggaac cacgcagttc
     2401 gtcggctgcg ccatcggcgg gatctcggtg ggcgaggtga tgcgcaccat cctgcccttc
     2461 tactcggcgc tcctcgcggc gctattcctc gtgacctaca tcccgggctt ttcgatgtgg
     2521 ctgccgcacc tgctgctggg gactcgctga agcgcgatcg agaattcggt ggtagaagta
     2581 agcaatgacc ccagtcgacg atcccgacag gacacgctac ggcgcgctcg cccag
//
LOCUS       DASVII010000207         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_513119,
            whole genome shotgun sequence.
ACCESSION   DASVII010000207 DASVII010000000
VERSION     DASVII010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513119"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF309_02650"
     CDS             complement(<1..379)
                     /locus_tag="VF309_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465512.1"
                     /translation="MKRLEPAIDVAVVIAAIVFAWGLTRLALYPALGIPDTAPAILRP
                     ILGFLAAWAMLWKRGERWASLGLARPRSWGVAIGGAVALYLVDMALSQWVVPAIADIF
                     KPQPQVSFLEYVRGNVGGFVLWAA"
     gene            complement(394..831)
                     /locus_tag="VF309_02655"
     CDS             complement(394..831)
                     /locus_tag="VF309_02655"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7465513.1"
                     /translation="MSEDNPLVRKHSGDFRWQRVDVLAYKDDGAAPFKDVTRQVLFEG
                     RELPAQLRYFEVAPGGYTTLERHEHVHAVMVIRGKGQVLVGDKASDIGLHDLVNVPPM
                     TWHQFHAAKDEPLGFLCLVAADRDRPQLPAEHEREAIAKPLRK"
     gene            complement(859..>1662)
                     /locus_tag="VF309_02660"
     CDS             complement(859..>1662)
                     /locus_tag="VF309_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7465514.1"
                     /translation="DVRFPAGIENAFPGPRFGIEGIRAITGVRGRPLTCTALKPQGSS
                     VEYLAHLARTFALAGIDVIKDDHGIANQAQHPFAERVAAVQKAIAEANRETGGNTIYA
                     PTFSGGGRSLMDQARIAQEHAVRMALVAPMLVGIPAFVEMRADLDIALMAHPAFAGGN
                     RIAAPVLLGKLFRLFGADATIFPNHGGRFSYPRETCLEIARAAREPWADVRPTLPVPA
                     GGMGVERVAEMLATYGSDTMLLIGGGLLSARDRLLERSREFVRAVHALR"
BASE COUNT          264 a          579 c          580 g          239 t
ORIGIN      
        1 tcgccgccca aagcacgaag ccgcccacgt tgccgcgcac gtactcgagg aacgacacct
       61 ggggctgcgg cttgaaaata tcggcgatcg ccggcactac ccactgcgaa agcgccatgt
      121 cgacgagata gagcgcgacc gcgcccccga tcgccacgcc ccagctccgg ggacgcgcga
      181 gcccgagcga agcccaacgc tcgccgcgct tccacagcat cgcccacgcc gcgaggaagc
      241 ccaggatcgg gcgcaggatc gccggagcgg tgtcgggaat gccgagcgcc ggatagagcg
      301 cgaggcgagt gaggccccag gcgaatacga tcgccgcgat cacgacggcg acgtcgatcg
      361 cgggctcgag cctcttcacc gctcgagcgc agcctacttg cgaagcggct tcgcgatcgc
      421 ctcgcgctcg tgctccgcgg gaagctgcgg gcgatcgcgg tcggcggcga cgaggcacag
      481 gaacccgagc ggctcgtcct tcgcggcgtg gaactggtgc cacgtcatcg gcggcacgtt
      541 caccaggtcg tgcagcccga tgtcgctcgc cttgtcgccg acgaggacct ggcccttgcc
      601 gcggatcacc atcacggcat gcacgtgctc gtggcgctcg agcgtggtgt agccgcccgg
      661 cgcgacttcg aaatagcgca gctgcgcggg gagctcacgg ccttcgaaga gcacttgccg
      721 cgtcacatcc ttgaatggcg cggcgccgtc gtccttgtag gccagcacgt cgacgcgctg
      781 ccagcggaag tcgccggaat gcttgcggac caggggattg tcctcgctca tcgaggaatc
      841 ttaccttttc gctcgcggct agcgcagcgc gtgcacggcg cggacgaatt cgcggctgcg
      901 ctcgaggagc cggtcgcgcg ccgacaggag gcccccgccg atgaggagca tcgtgtcgct
      961 gccgtaggtc gcgagcatct cggcgacgcg ctcgacgccc atgccgccgg cgggcaccgg
     1021 cagcgtgggg cgaacgtccg cccacggctc gcgcgcggcg cgggcgatct cgaggcaggt
     1081 ctcgcggggg tagctgaagc gcccgccgtg gttggggaag atcgtcgcgt cggcgccgaa
     1141 gagccggaag agcttgccca gcagcacggg cgcggcgatc cgattgccgc ccgcgaaagc
     1201 cggatgcgcc atcaacgcaa tatcgaggtc ggcccgcatc tcgacgaaag ctggtatgcc
     1261 cacgagcatc ggtgcgacca acgccatcct caccgcatgc tcctgcgcaa tcctcgcctg
     1321 gtccatgagc gagcgtccgc cgccggagaa ggtcggcgcg tagatcgtgt tgcctcccgt
     1381 ttcgcgattg gcctcggcaa tggccttctg caccgccgcg acgcgctcgg cgaacggatg
     1441 ttgcgcctgg ttcgcgatgc cgtggtcgtc cttgatgacg tcgatgcccg cgagcgcgaa
     1501 ggtgcgcgcg aggtgcgcga gatactccac cgacgagccc tggggcttca gcgccgtgca
     1561 cgtgagcggc cgtccgcgca ccccggtgat ggcgcggatc ccctcgatgc cgaagcgcgg
     1621 gccggggaat gcgttctcga tccccgcggg gaagcgcacg tc
//
LOCUS       DASVII010000208         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_585354,
            whole genome shotgun sequence.
ACCESSION   DASVII010000208 DASVII010000000
VERSION     DASVII010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585354"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VF309_02665"
     CDS             complement(<1..847)
                     /locus_tag="VF309_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7465515.1"
                     /translation="MKKALKVVGYVVGGIVVILLVLYGFFAYSRISTIHAARNKMGRP
                     ADTLRVGGFAFRDLNKNGKLDPYEDRRQPTAVRVENLLGQMTLDEKAGTMFHTFVWPG
                     KNGEIAGPLQVLNLLPIEVALFDKRITFFNLFGLGTAAETAHWLNAVQRLAERTRLGI
                     PVTISSDPRHSAALQKSVAAFAMPEFSQWPEPIGFAAIGDSALVVEFGRFAAQDYRAI
                     GIQVALHPMADVATEPRWGRIRGTFGEDANLVAKLTAAYVRGMQGDSLSPTSVATVTK
                     HFAGGG"
     gene            931..1845
                     /locus_tag="VF309_02670"
     CDS             931..1845
                     /locus_tag="VF309_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465516.1"
                     /translation="MSRPVGVTASAVVAILGSILALLFAGIMVATPYIAPAQAQPPNG
                     AQFIIAAAGMFAGLAAIGIWTSVGLFRLHSWARTSMLVFAGFLAAVSIFALMGTMAVP
                     MPPEYTAATVRTFHRTIALMFGIPFAVGVWWLIQFNTQSTKAAFASPIAEAASLRPLS
                     ITIIAWVSIAGGAFCLFPMLAGMPAFLFGASFNGWIAGVIYAFFGALSLYIGKGLLDL
                     RERARLIAIGWFGFSLVHAGAVTLVPTLRQRLLESQRALTQNQPNPIPFDQGMMLNMI
                     FAFAAIVAAVTIWFLIRNRAAFVRAEIR"
     gene            1951..>3002
                     /locus_tag="VF309_02675"
     CDS             1951..>3002
                     /locus_tag="VF309_02675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7465517.1"
                     /translation="MLARDLSLERPVALKLLPAMMAAQPVLRERFLREARTAAGLSHP
                     HIVPIHAVEAHEVIVFIAMAYVDGETLADRVRRTGPLPPHEVARILREVAWALAYAHG
                     RGIVHRDIKPENILIERGSGRAMVTDFGIARARNGSDVANRLTLEGQLLGTAAFMSPE
                     QGADEPVDGRSDLYALGGVGYFALVGRAPFEATTLEAMLVARYTRAAPPVASARPDVP
                     PALAAAIDRCLAREPHNRPASAEVVAEALSEATGIRGTQYIALPVRSFLRAAEQSVWL
                     ATLVVVFTLIYGLPGTRNLLALIAGIVFGVALLSIDLVRRARELQGEGFGAADVRRGF
                     EVERATHAEELRQLFDA"
BASE COUNT          469 a          993 c          976 g          564 t
ORIGIN      
        1 gaccgccgcc cgcaaaatgc ttggtaaccg tggcgacgct ggtcggcgag agcgagtcgc
       61 cctgcatgcc gcgaacgtac gccgccgtca atttcgcgac gaggttggcg tcctcaccga
      121 acgtcccgcg gatgcgcccc cagcgcggct cggtcgcaac gtccgccatc gggtgcagcg
      181 cgacctgaat gccgatcgcg cgatagtcct gcgccgcaaa gcgaccgaac tcgacgacca
      241 gcgcgctgtc gccgatcgcc gcgaacccga tcggctccgg ccactgtgag aactcgggca
      301 tcgcgaacgc ggcgaccgac ttctggagcg cggcggagtg cctggggtcg gagctgatcg
      361 tgacggggat gcccagacgc gtccgctccg ccagtcgctg caccgcgttg agccagtgcg
      421 cggtctccgc ggcggtgccg agcccgaaca ggttgaagaa cgtaatcctc ttgtcgaaga
      481 gcgcgacctc gatcggcagc aggttcaaca cctggagcgg gcccgcgatc tcaccgttct
      541 ttcccggcca cacgaaggtg tggaacatcg tgccggcctt ctcgtcgagg gtcatctgac
      601 ccagcaggtt ctccacgcgg acggctgtcg gctgccggcg atcctcataa ggatccagct
      661 ttccgttctt gttcaggtcg cggaacgcga agccgcccac ccgaagcgtg tcggcgggtc
      721 gacccatctt gttgcgcgcg gcgtgtatcg tggagatcct gctgtaggcg aagaatccgt
      781 acagcacgag tagaatgacg acgattcccc ccacgacata gccgactact ttcagtgcct
      841 ttttcatact cgcgccgcgc ctcggtcaga tagcgtggtc agaaattcga gagctcgggc
      901 aaattgtcaa taaagaaata gattattcgc atgagcagac ccgtcggcgt caccgcgagc
      961 gcggtagtag cgatcctcgg aagcatcctc gcgctgctgt tcgcggggat catggttgca
     1021 accccgtaca tcgcgccggc gcaggcccaa ccgcccaacg gtgctcagtt tattatcgcg
     1081 gctgctggga tgtttgccgg gctcgccgcc attggcattt ggacttccgt cggacttttc
     1141 cgcctccact catgggcgcg cacatcgatg ctggtcttcg ccggtttcct cgcggcggtc
     1201 tccattttcg ccctgatggg gacaatggcc gtgcccatgc ctccggaata caccgccgcc
     1261 acggttcgca cttttcaccg aacgatcgcg ttgatgttcg gaattccgtt cgcagttggc
     1321 gtgtggtggc tcatacagtt caacacgcag tcgacgaaag ccgcctttgc ttcccccata
     1381 gccgaggccg cgtctctgag gccgctgagc atcacgatca tcgcctgggt gagcatcgcc
     1441 ggaggcgcgt tctgcctctt tccgatgctg gcgggcatgc ctgcgttttt gttcggggcg
     1501 agtttcaatg gttggatcgc gggcgtcatc tacgctttct tcggagccct atcgctgtac
     1561 atcgggaagg gtttgctcga tctgcgggag cgggctcggc ttatagcgat cgggtggttc
     1621 gggttctcgc ttgttcatgc gggcgccgtc acactcgtgc cgactttgcg ccagcgactg
     1681 ctcgagtcgc aacgagcgct cactcaaaat cagccgaacc cgattccatt cgatcagggc
     1741 atgatgctga acatgatatt tgccttcgct gcgattgtgg cagcagtcac catctggttc
     1801 ctgatacgaa atcgcgcggc gttcgtgcgg gcggagatcc gctaaggccg ggtgatcgag
     1861 accagcccgg accttcagac tctcgagcga gcggtcgcgg gccgctacgc gctcgagcgc
     1921 gagcttggcc gcggcggcat gggtgtcgtc atgctcgccc gcgatctctc gctcgaacga
     1981 cccgtggcgc tcaagctgct ccccgcgatg atggcggcgc agcccgtgct ccgcgagcga
     2041 ttcctgcgcg aggcacgcac cgcggccggc ctctcgcatc cgcacatcgt gccgatccat
     2101 gccgtcgagg cgcacgaggt gatcgtgttc atcgcgatgg cctacgtcga cggcgagacg
     2161 ctcgccgacc gcgtgcggcg tacaggtccg ctgccgccgc acgaggtggc gcgcatcctc
     2221 cgcgaggtcg cctgggcgct ggcgtatgcg catggccgtg gcatcgtgca tcgcgacatc
     2281 aagcctgaga acatcctgat cgagcgcggc agcggtcgcg cgatggtcac cgacttcggg
     2341 atcgcgcgtg ccaggaatgg ctccgacgtg gccaatcggc tcacgctcga ggggcagctg
     2401 ctcggcaccg cggcgttcat gagtcccgaa cagggtgcgg acgagcccgt cgatggacgc
     2461 agcgatctct acgcactcgg cggagtcggc tacttcgcgc tcgtcgggcg ggcgccgttc
     2521 gaggcgacga cgctcgaggc gatgctcgtc gcgcgctaca cgcgcgcggc gccgccggtc
     2581 gcatcggcac gacccgacgt gccacccgcg ctcgccgccg cgatcgaccg ctgcctcgcg
     2641 cgggaaccgc acaacaggcc cgcctccgcg gaagtggtcg ccgaggcgct gtcggaggcg
     2701 actggaataa ggggcaccca gtacatcgcg ctccctgtgc gcagcttcct tcgcgccgcc
     2761 gagcagagcg tctggctcgc cacgctcgtc gtggtgttca cgctgattta cgggcttccc
     2821 gggacgcgga atcttctcgc gctcatcgcg ggcatcgtct tcggcgtcgc gctgctctcg
     2881 atcgacctcg tgcggcgtgc gcgcgagctc cagggcgaag ggttcggcgc cgcggatgtg
     2941 cgtcgcggat tcgaggtcga gcgggcgacg cacgcggaag agctgcgcca gctgttcgat
     3001 gc
//
LOCUS       DASVII010000209         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_549690,
            whole genome shotgun sequence.
ACCESSION   DASVII010000209 DASVII010000000
VERSION     DASVII010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549690"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1419
                     /locus_tag="VF309_02680"
     CDS             130..1419
                     /locus_tag="VF309_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX7465518.1"
                     /translation="MSNRIDRRRFVLAAASSLAAATLPAAVRAAMGPNDKFDLVIRGG
                     EVLDPSQRLRGVRDIGIKNGIVEAVEVGIPAERALRTIDATGKLVTPGLIDLHAHTYP
                     YGSAIGIPADELVPFQGTTTAISAGDAGANNFAAFRRFIVGSTRTRLYAFVHIANIGL
                     AGFPIAELYNMDYARTEDAARTLAENADIAIGIKVRMSENVIAKNGLEPLKRAIKACE
                     LSGVAGAKVMCHIGGVENRKLMAEILDTLRPRDILTHCYSGAPNLHDDFTNIVQDGKI
                     LPAALEAKRRGVHFDVGHGGGSFDYTIAEAAIAAGASPDTISSDMHVFSGNTPGMPYL
                     TWVMSKFLNMGFTLEQVIEMTTIAPSRAIGKIAKLGTLQVGAPGDVTILEVVNGPVEF
                     VDTRNNKRKGNVFIRPVQTVNGGIAFGRPFSAPFSVR"
     gene            1447..>1680
                     /locus_tag="VF309_02685"
     CDS             1447..>1680
                     /locus_tag="VF309_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7465519.1"
                     /translation="MAESVAFILNGRSAQSNAKGSTPLVYVLRGEMGLMGARIGCGEG
                     QCGSCTVIVDGKAVTSCDLPLEAVAGKSVETVEG"
BASE COUNT          297 a          565 c          544 g          274 t
ORIGIN      
        1 cgttttggta gtggctttca tggtgatgcg gcgatcaaag gtgtcgattc taacgttctt
       61 gacctcgcgc ggtcgccggt tcaccatcgg ccggcgccaa aaaaacaaaa cttccccgga
      121 ggaaacacca tgagcaaccg gatcgatcgc cgccgcttcg ttctcgccgc cgcttcctcg
      181 ctcgccgccg cgacgctgcc ggccgccgtg cgcgccgcga tggggcccaa cgacaagttc
      241 gacctcgtga tccggggcgg cgaggtcctc gatccgagcc agcgcctgcg cggcgttcgg
      301 gacatcggca tcaagaacgg gatcgtcgag gcggtcgagg tgggaattcc cgcggagcgc
      361 gcgctgcgaa ccatcgacgc gaccggaaag ctcgtgacgc ccgggctcat cgaccttcac
      421 gcgcacacct atccctacgg ctccgccatc ggcattcccg ccgacgagct cgtgccgttc
      481 caggggacga cgacggcgat ctccgcgggt gacgcgggcg cgaacaattt cgccgcgttc
      541 cggcgcttca tcgtgggctc gacgcgcacg cgcctctacg ctttcgtgca catcgccaac
      601 atcggcctcg cgggcttccc gatcgccgag ctctacaaca tggactacgc gcgcacggaa
      661 gacgccgcgc gcacgctcgc ggagaacgcc gacatcgcga tcgggatcaa agtgcgcatg
      721 tcggagaacg tgatcgcgaa gaacggcctg gagccgctca agcgcgccat caaggcctgc
      781 gagctctcgg gcgtggccgg cgccaaggtg atgtgccaca tcggcggcgt cgagaaccgc
      841 aagctcatgg cggaaattct cgatacgctt cgcccgcgcg acatcctcac gcactgctac
      901 tcgggggcac ccaacctcca cgacgacttc accaacatcg tccaggacgg gaagatcctc
      961 ccggcggcgc tcgaggccaa gcggcgcggc gtgcacttcg acgtggggca cggcgggggc
     1021 tccttcgact acaccatcgc cgaggccgcg atcgccgcgg gcgcttcgcc ggacacgatc
     1081 tcctcggaca tgcacgtctt ctcgggtaac acgcccggca tgccttacct cacgtgggtg
     1141 atgagcaagt tcctgaacat gggatttacg ctggagcagg tgatcgagat gacgaccatc
     1201 gcgccgtcgc gcgccatcgg caagatcgcg aagctcggca cgctgcaggt gggcgctccc
     1261 ggggacgtca ccatcctcga ggtggtcaac gggcccgtgg agttcgtcga cacgcgcaac
     1321 aacaagcgca agggcaacgt gttcattcgc cccgtgcaga cagtcaacgg cggcatcgcg
     1381 ttcggacgcc ccttttcggc gccgttcagc gtaaggtagt ctttcaatat ccaaaggagc
     1441 gtcgcggtgg ccgagtccgt tgccttcatc ctgaatggcc gttccgcgca gtcgaacgcg
     1501 aagggctcga cgcccctcgt ctacgtgctg cgcggcgaga tgggcctcat gggcgcgcgc
     1561 atcggctgcg gcgagggtca atgcgggtcg tgcacggtga tcgtcgacgg gaaggcggtc
     1621 acatcgtgcg acctgccgct cgaggcggtg gccggaaaat ccgttgagac cgtcgaggga
//
LOCUS       DASVII010000210         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_549736,
            whole genome shotgun sequence.
ACCESSION   DASVII010000210 DASVII010000000
VERSION     DASVII010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549736"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1033
                     /gene="ntrB"
                     /locus_tag="VF309_02690"
     CDS             104..1033
                     /gene="ntrB"
                     /locus_tag="VF309_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HEX7465520.1"
                     /translation="MNAINRSLDPARPDDASRRIVAHPSALRAKAQMTIPAPSPIAPM
                     NRVTSNRAPLPTWFVRPLAMALGILCFVALWALIAKAGRIPDPPSVFKEALRLFANPF
                     YSNGPNDQGIGWNVLRSLGRVAIGFGLAAVVGIPLGFMIGRFRFLADMAAPIIALLKP
                     VSPLAWLPIGLLVFKAANPASLYVIFFCSLWPMIVNTAVGVRQIPQDYLNVARVLNLS
                     EWKVFTKILLPAVLPYMLTGVRLSIGVAWLVIVAAEMLTGGVGIGFWVWDEWNNLKVD
                     HILIAIFVIGMVGLALEQALIFVAKRFSHGVAA"
     gene            1043..>1792
                     /locus_tag="VF309_02695"
     CDS             1043..>1792
                     /locus_tag="VF309_02695"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465521.1"
                     /translation="MDKYLRVESVGMVFDTRGKRFEALRDISLTVRRGEFVSLIGHSG
                     CGKSTLLNLVAGLLAPTEGLLLLNNKEIPGPGPERGVVFQNHSLLPWLTCAGNVHLAV
                     ERVFGENESKARLAERTLAALALVGLTQAAGKYPDEISGGMKQRVGIARALAMEPKIL
                     LLDEPFGALDALTRAHLQDELMAIVANTGSTVVMVTHDVDEAVLLSDRIVMMTNGPAA
                     TIGEILRVDLQRPRNRVELAEDSDYTHFRKAV"
BASE COUNT          275 a          611 c          596 g          310 t
ORIGIN      
        1 gctgatgcgc acctcgaagc tgatggacgg cgtgatatgg gacggcaagg acccggtgaa
       61 gtacgcgggc aacttcaagg tgaaggtcgc ctgaggcgcc gggatgaacg cgatcaaccg
      121 ctccctcgac cccgcgcgcc ccgacgacgc ttcgcggcgc atcgtggccc atccttccgc
      181 gctgcgggcg aaggcgcaga tgacgattcc cgcgccctcc ccgatcgcgc ccatgaatcg
      241 cgtcacgtcg aaccgcgcgc cgctgcccac atggttcgtg cgtccattgg cgatggccct
      301 gggcatcctg tgcttcgtcg cgctgtgggc cctcatcgca aaggcaggcc gcattcccga
      361 tccaccctcg gtcttcaagg aggcgctgcg gctcttcgcc aatcccttct acagcaacgg
      421 ccccaacgac cagggcatcg gatggaacgt gcttcgctcg ctcgggcgcg tcgcgatcgg
      481 tttcgggctc gccgccgtgg tggggattcc gctgggcttc atgatcgggc gttttcgatt
      541 cctcgccgac atggcggcgc ccatcatcgc cctcctcaag cccgtgagcc cgctcgcgtg
      601 gctgcccatc gggctcctcg tgttcaaggc cgcgaacccc gcgtcgctct acgtgatctt
      661 cttctgctcg ctctggccga tgatcgtgaa caccgccgtc ggcgtgcgcc agatccccca
      721 ggactacctc aacgtcgcgc gcgtgctgaa tctttccgag tggaaggtgt tcacgaagat
      781 cctgctgccc gcggtgcttc cctacatgct cacgggcgtg cgcctttcga tcggggtggc
      841 gtggctggtg atcgtcgccg cggagatgct caccggcggc gtgggcatcg gcttctgggt
      901 gtgggacgag tggaacaacc tgaaggtcga ccacatcctc atcgcgatct tcgtgatcgg
      961 catggtggga ctcgcgctcg agcaggctct catcttcgtg gcgaagcgct tcagccacgg
     1021 cgtcgcggcg taggcggcgg ccatggacaa atacctgcgg gtcgagtcgg tcggcatggt
     1081 gttcgacacg cgcggcaagc gcttcgaagc gctgcgcgac atctcgctca ccgtgcggcg
     1141 cggcgaattc gtgagcctca tcgggcactc ggggtgcggg aagtcgacgc tcctgaacct
     1201 cgtcgcgggc cttctcgcgc ccacggaggg cctgctcctg ctcaacaaca aggaaatccc
     1261 ggggcccggc cccgagcgcg gcgtcgtctt ccagaaccat tcgctgctgc cgtggctcac
     1321 ctgcgcgggc aacgtgcacc tcgccgtcga gcgcgtcttc ggcgagaacg agtcgaaagc
     1381 gcggctcgcc gagcgcacgc tcgcggcgct cgcgctggtc gggctcacgc aggccgcggg
     1441 caagtatccc gacgagatct ccggaggaat gaagcagcgc gtcggcatcg cgcgcgccct
     1501 cgcgatggag ccgaagatcc tgctcctcga cgagcccttc ggagcgctcg acgcgctcac
     1561 gcgcgcgcac ctgcaggacg agctgatggc gatcgtggcg aacaccggat cgacggtggt
     1621 gatggtgacg cacgacgtcg acgaggcggt gctgctgtca gaccggatcg tgatgatgac
     1681 gaacggcccg gcggcgacga tcggcgagat cctgcgcgtc gacctgcagc ggccgaggaa
     1741 ccgcgtcgag ctcgccgagg acagcgacta cacgcacttt cgcaaggccg tg
//
LOCUS       DASVII010000211         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_298017,
            whole genome shotgun sequence.
ACCESSION   DASVII010000211 DASVII010000000
VERSION     DASVII010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298017"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VF309_02700"
     CDS             <1..976
                     /locus_tag="VF309_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7465522.1"
                     /translation="AFVCGMALAASAVAQVGEHTFRFTTANPAGHPIVTGAEKFAQLV
                     SEKSGGKMTVKLFPGGVLGGDVQVLSAVQGGTIDVTSMNSGILQGQVKEFAIVDLPFL
                     FNNPREADAVLDGPIGKALADKLPEKGLIHLAYYELGFRNLTNSKRPVKSADDIAGLK
                     IRVIQSPIYIDTFSALGANPVPMPFPEVYTALEQKTIDGQENPFSVIEANKFAEVQKY
                     LSITRHVYNPQTLMFSKKAWDRLSKDEQAIIAAAASESATYERKVARDASDQSLANLK
                     KTMEVNEVSPAELAKIREKLKPVIDKYSAQVGPEFVARLYAEIAKARK"
     gene            977..1813
                     /locus_tag="VF309_02705"
     CDS             977..1813
                     /locus_tag="VF309_02705"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX7465523.1"
                     /translation="MTISGKTTLIAHIGYPTEAFKAPLIYNPWFEKRGIDAVVVPMGV
                     KVEDYPAAVMALFKLTNIRGALITMPHKVVTGRLVNEITRAVEVAGACNAILKRPDGT
                     LVGNMFDGAGFTRGLVHKGFSCKGAKCLIVGCGGVGSAIAASLAEEGARSVALFDLDA
                     EAMTQLATRLRRYYPGTAIVEGSRDPAGYDLAVNCTPLGMKAGDPLPMDATRLAPSTF
                     VGEVVMSMEITPLLAAARERGCRIQVGIDMLFEMIPAYLEFFGFGAATADELRAVAKI
                     TY"
BASE COUNT          367 a          591 c          633 g          283 t
ORIGIN      
        1 agcattcgtc tgtggcatgg cgctcgcggc gagtgccgtg gcgcaggtgg gagagcacac
       61 gttccgcttc acgaccgcga accccgccgg gcacccgatc gtgaccgggg cggagaaatt
      121 cgcgcagctg gtgagcgaga agagcggcgg caagatgacg gtcaagctct tccccggtgg
      181 agtcctcggc ggagacgtcc aggtgctttc cgcggtgcag ggcgggacca tcgacgtgac
      241 ctcgatgaac tcgggaattc tccagggaca ggtcaaggaa ttcgcgatcg tcgacctgcc
      301 gttcctcttc aacaacccga gggaggcgga cgcggtgctg gacggcccca tcggcaaggc
      361 gctcgccgac aagctgcccg agaagggcct catccacctc gcgtactacg agctgggctt
      421 ccgcaacctc accaacagca agcgcccggt gaagagcgcc gatgacatcg cggggctgaa
      481 aatccgcgtg atccagtcgc cgatctacat cgacaccttc agcgccctgg gcgcgaaccc
      541 ggtgcccatg ccgtttcccg aggtctacac cgccctcgag cagaagacca tcgacggcca
      601 ggagaatccg ttcagcgtga tcgaggccaa caagttcgcc gaggtgcaga agtacctgag
      661 catcacgcgg cacgtctaca atccgcagac cctgatgttc tcgaagaagg cctgggaccg
      721 gctgtcgaag gacgagcagg cgatcatcgc ggcggcggcg agcgaatcgg ccacatacga
      781 gcgcaaggtc gcgcgcgacg cgagcgacca gtcgctcgcg aacctgaaga agaccatgga
      841 agtgaacgag gtctctcccg cggagctggc gaagattcgc gagaagctca agcccgtcat
      901 cgacaagtac agcgcgcagg tcgggccgga gttcgtcgcg cggctctatg cggagatcgc
      961 gaaggcccgc aagtaaatga ccatcagcgg caagaccact ttgattgcgc acatcggcta
     1021 cccgaccgag gcgttcaagg cgccgctcat ctacaacccg tggttcgaga agcgcggcat
     1081 cgacgcggtc gtcgtgccga tgggcgtgaa ggtcgaggac tacccggccg cggtgatggc
     1141 gctcttcaag ctcaccaaca tccgcggggc gctcatcacg atgccgcaca aggtcgtcac
     1201 gggaaggctg gtcaacgaga tcacgcgggc ggtggaagtg gcgggggcct gcaacgcgat
     1261 cctgaagcgc cccgacggca cgctggtggg caacatgttc gacggcgcag gattcacgcg
     1321 cggcctggtg cacaagggct tttcgtgcaa gggcgccaag tgcctgatcg tcggatgcgg
     1381 cggcgtggga tcggcgatcg cggcctcgct cgcggaggag ggcgcgcgat cggtcgcgct
     1441 cttcgacctc gacgcggagg ccatgacgca gctcgccacg cgcctgcggc gatactatcc
     1501 gggcaccgcg atcgtcgaag gatccaggga cccggcgggc tacgacctcg ccgtcaactg
     1561 cacgcctctc ggaatgaagg ccggcgatcc cctgccgatg gacgcgacgc gcctcgcgcc
     1621 ctcgactttc gtgggcgaag tggtgatgag catggagatc acgccgctcc tggccgcggc
     1681 gcgggagcgc ggatgccgga tccaggtcgg catcgacatg ctcttcgaga tgatcccggc
     1741 gtacctcgaa ttctttgggt tcggcgcggc caccgccgac gagctgcgcg cggtggcgaa
     1801 gatcacctac tgaggaaaag ggcatgaaga agatcctggt gctccatggc gtgaacctca
     1861 acatgttcgg caag
//
LOCUS       DASVII010000212         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_153891,
            whole genome shotgun sequence.
ACCESSION   DASVII010000212 DASVII010000000
VERSION     DASVII010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153891"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1039)
                     /locus_tag="VF309_02710"
     CDS             complement(50..1039)
                     /locus_tag="VF309_02710"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7465524.1"
                     /translation="MKKPVRVAVTGAAGQIGYSLLFRIASGEMLGKEQPVILQLLEIP
                     DEKAQKALKGVMMELDDCAFPLLQSMVAASDPKVAFRDVDVALLVGARPRGPGMERKD
                     LLEANGAIFVPQGKALDEVASRDVRVLVVGNPANTNSLIAMKNAPSLKPQQFTAMMRL
                     DHNRALTQVAQKLGKPVTAVKKMTIWGNHSATQYPDLFQAEAEGKKVWPMINDQAWLE
                     NTFIPTVQKRGAAIIEARGLSSAASAANAAMDHVRDWVNGTPAGDWVSMGIPSDGSYG
                     IPEGLMFGYPTTCSGGRYEIVKGLDLGDFARGKIAATHKELQEERAAIQHLLK"
     gene            complement(1162..2067)
                     /locus_tag="VF309_02715"
     CDS             complement(1162..2067)
                     /locus_tag="VF309_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7465525.1"
                     /translation="MNPQQRQSRLVGALLVAAGAVAFSGKGVIIKLAYRHGVDAVTLL
                     ALRMLLSAPFFAALGLWAARRGDVEPLSWADRRTLVVLGLIGYYLASYFDFLGLQYVT
                     AALERLLLFVHPTFVVLFSAMLFGRRITRRDVLAIALSYLGIFVVFGNDLATQPGNVA
                     LGSFWVLLSALFYAAYLIGSGRLVGRVGTLRFACYAGLISSVAVVTHFALTRDVALLF
                     AQAAPVYWLALLMALASTVLPIVMTMEGIRRIGAAHASMVASVGPMATIFLGAIFLGE
                     RITAVQVAGALLVLAGVLSISLRRK"
     gene            complement(2077..3084)
                     /locus_tag="VF309_02720"
     CDS             complement(2077..3084)
                     /locus_tag="VF309_02720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7465526.1"
                     /translation="MTAIVRVAVIVVTLALFLGSSLNAITGHRDLAVLFALATPLGIS
                     AWGFARAGHNEAAVALLCCVLITVVTMVLVLNPLGVRDVAITAYGGIVLVGALLLSRR
                     SFFALTALLLIAASTAFLMDLNGFTRSEIARYTDWSSFAEFLVIIGVFAALGRYGSEV
                     LFGSLGDAHRASSDDAVSGVANRSGFFTLAAAKLKALQGSPTCSVLVLVDLDGFRRVN
                     VVIGHRAADGVLLETALRMRKAIGAGHVLARIGDDEFAALGLGLRDEALAHEFARAVH
                     EAVQFEFSGVSVRASTGYARFPRDAHGLESLLLAAEGSLARAKDHVHEVDRFTGPADR
                     I"
BASE COUNT          599 a         1111 c         1119 g          467 t
ORIGIN      
        1 atcgccgtcg ataccgactg aaatccattt cgccgcagcc tgcacccggt cacttcagca
       61 ggtgctggat tgcggcgcgc tcttcctgca gctccttgtg ggttgcggcg atctttcccc
      121 tggcgaagtc gccgagatcc agccccttca cgatctcgta gcgcccgccc gagcaggtgg
      181 tggggtagcc gaacatcagg ccctcgggaa tgccatagct gccgtccgaa gggattccca
      241 tcgagaccca gtctcccgca ggcgtgccgt tcacccaatc gcgaacgtgg tccatcgccg
      301 cattggccgc cgacgccgcc gacgagagcc cgcgcgcctc gatgatcgcg gcgccgcgct
      361 tctgcacggt cggaatgaag gtattttcca gccaggcctg gtcgttgatc atcggccaga
      421 ctttctttcc ttccgcttcg gcctgaaaca ggtcggggta ctgggtcgcc gaatggttgc
      481 cccagatggt catcttcttc acggcggtca ccggcttgcc gagcttctgc gcgacctgcg
      541 tgagcgcgcg gttgtgatcg agccgcatca tcgcggtgaa ctgctggggc ttgagcgacg
      601 gcgcgttctt catcgcgatg agcgagttgg tgttggcggg attgccgacc acgagcaccc
      661 gcacgtcgcg ggaagccacc tcgtcgagcg ccttgccctg cggcacgaag atcgcgccgt
      721 tcgcctcgag gagatccttc ctctccatgc ccgggccgcg gggccgcgcg ccgacgagga
      781 gcgcgacgtc cacgtccctg aacgcgacct tcggatcgga agccgcgacc atcgactgca
      841 gcagcggaaa ggcgcaatcg tcgagctcca tcatcacgcc cttcagcgcc ttctgcgcct
      901 tctcgtcggg gatctcgagc agctgcagga tcaccggctg ttccttgccg agcatctcgc
      961 cgctggcgat ccggaacaac aggctgtagc cgatctggcc ggcggcgccg gtcacggcga
     1021 cgcgtacggg cttcttcatt gcaactcctt gggagtcggg acggaattcg agccgcgatt
     1081 ataaaccggg gacccacccg cgaaattcgc accgcgaaat tcgtaccggg tacgaattcg
     1141 aaaatgcgta cctgacacga attatttcct ccgcagcgag atcgacagga cgccggcgag
     1201 cacgagcaat gcgccggcca cctgcaccgc ggtgatgcgc tcgccgagga agatcgcgcc
     1261 gaggaagatg gtcgccatgg ggccgaccga ggcgaccatc gaggcgtggg cggcgccgat
     1321 gcggcggatg ccttccatcg tcatcacgat cggcaacacg gtcgaggcca gcgccatcag
     1381 cagcgcgagc cagtagaccg gcgcggcctg cgcgaacagg agggcaacgt cgcgcgtgag
     1441 cgcgaagtgc gtcaccaccg ccaccgagga gatcagcccc gcgtagcagg cgaagcgaag
     1501 cgtcccgacg cggcccacca gccgcccgct tccgatcagg taggccgcgt agaagagcgc
     1561 cgacagcagc acccagaagg atccgagcgc gacgtttccc ggctgggtcg cgaggtcgtt
     1621 gccgaacacg acgaatatgc ccaggtacga aagcgcgatc gcgagcacgt cgcggcgcgt
     1681 gatgcgccgc ccgaagagca tcgccgagaa gagcacgacg aaggtcggat gcacgaacag
     1741 cagcaggcgt tcgagcgccg ccgtcacgta ctgcagcccg aggaagtcga agtagctcgc
     1801 gaggtagtag ccgatgagcc ccagcaccac gagcgtgcgc cggtcggccc acgagagggg
     1861 ttccacgtcg ccgcggcgtg ccgcccacaa ccccagggcc gcgaagaacg gcgcgctcag
     1921 cagcatgcgc agcgccagca gggtgacggc gtcgacgcca tggcgatagg cgagcttgat
     1981 gatgacgccc ttgccggaaa aggcgacggc gccggcggcg acgaggagcg cgccgacgag
     2041 gcgcgattgg cgttgctgcg gattcaacta ggaagcctag atgcggtcgg cgggacccgt
     2101 gaagcgatcg acctcgtgca catggtcctt cgcgcgggcg agcgagcctt cggcggcaag
     2161 gagcagcgat tctagcccgt gggcgtcgcg cgggaagcgc gcgtagccgg tcgacgcgcg
     2221 caccgacacg ccggagaatt cgaactgcac cgcctcgtgc accgcgcgcg cgaactcatg
     2281 ggcgagcgcc tcgtcgcgca ggccgaggcc gagcgccgcg aactcgtcgt cgccgatccg
     2341 cgcgagcacg tgccccgcgc cgatcgcctt cctcatccgc agcgccgtct ccagcagcac
     2401 cccatcggcc gcgcgatggc cgatcaccac gttcacgcgc cggaagccgt cgaggtcgac
     2461 gagcaccagc acgctgcagg tgggcgagcc ctgcagggcc ttcagcttcg ccgcggcgag
     2521 cgtgaagaag ccgctgcgat tggcaacgcc gctcacggca tcgtcgctcg aggcgcggtg
     2581 cgcgtccccg aggcttccga agagcacctc ggagccgtag cgcccgagcg cggcgaagac
     2641 cccgatgatc accaggaact ccgcgaagct cgaccagtcc gtgtagcggg ctatctcgct
     2701 gcgagtgaag ccgttcaggt ccatcaggaa agcggtggac gcggcgatga gcagcagcgc
     2761 cgtgagcgcg aagaaggagc ggcgcgacag gagcagcgcc cccaccagga cgatgccgcc
     2821 ataggccgtg atcgccacgt cgcgcacgcc gagcggattc aggaccagca ccatcgtgac
     2881 cacggtgatg agcacgcagc acaggagcgc gacggccgcc tcgttgtggc cggcgcgagc
     2941 gaatccccac gccgagatgc cgaggggcgt ggcgagcgcg aacagcaccg cgaggtcgcg
     3001 gtgccccgtg atcgcgttga gcgaggagcc gaggaaaagc gcgagagtga cgacgatgac
     3061 cgcgacgcga acaatcgcgg tcatcggcgc gctcgccgaa tggtcgtgct ggagcggcat
     3121 ttagggagct acgcggacgg tgtagaggac tgtaccgctt ccgcccgccg cgagcgagcc
     3181 ggtcaacgtc cacacgacgg atcccgatcc attcaccgcc ggctgagtcg tcagggcaca
     3241 cgccgtgatg ttgccgggca gcggcgcgcg gcagctcgcg gcctggaagc tcgtga
//
LOCUS       DASVII010000213         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_621521,
            whole genome shotgun sequence.
ACCESSION   DASVII010000213 DASVII010000000
VERSION     DASVII010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621521"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..807)
                     /locus_tag="VF309_02725"
     CDS             complement(184..807)
                     /locus_tag="VF309_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX7465527.1"
                     /translation="MDALATRVALHRTLPPEEAARGDFYALFARLLHSGPDRALLGSI
                     AGADPIPGTSALGAAWRDLVDASCAMDADAAQEEFEALFVGVGKAEVSLYAGFYTGAP
                     AIDHPRVRIQADLAAFGLARREDAPEPEDHFAGLMDAMRVLVAGGAGRASATLADQRG
                     FYEAHIGPGIASFFEALARSKKANYYRKVAALGTAYFALETESFSLD"
     gene            complement(807..>1754)
                     /locus_tag="VF309_02730"
     CDS             complement(807..>1754)
                     /locus_tag="VF309_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7465528.1"
                     /translation="LLSAYRHAGGEDACIVFHNGHDGRELLAAWASSGTGLPARALPL
                     ETWHVSAIGLDLLLPAIAFGANQVVVISAGSEDVEYREALRSQMAIAEAILAGLGYGG
                     THFALVEASDTGRLEQAFARLSPAQVPPSSATFLLGNDKRTAIEFAVEHLAKLAPVKA
                     DVIALPAGSPFGEVVVDKARCTMCLSCVGACPVSALMDGVDKPLLKFLERNCVQCGLC
                     EATCPEDAISLAPRLLLTPQAREARVLNEAQPFHCVSCAKPFGTKQMVEAMLGRLAGH
                     SMFSSDGALRRLQMCADCRVVDMMSNKNEASVLRLGADS"
BASE COUNT          282 a          597 c          621 g          254 t
ORIGIN      
        1 tacgcggtcg tcgtcgattt ctcgcgggct tccgcgacgg gcgcggcctc cttcgccgtg
       61 cgcgacccgg tcacgacggc ggcggcgact ccggcgccgc ccagcccggc ggccagcagg
      121 aacttgcggc ggtgggtcgg cttggtggtc ttcatcgtgg gcctcctgtg gcgagacatg
      181 cgtttaatcg agggagaacg attcggtttc gagcgcgaaa tacgccgtgc cgagcgcggc
      241 gaccttgcgg tagtagttgg ccttcttcga gcgcgcgagc gcctcgaaga agctcgcgat
      301 gccgggcccg atgtgagcct cgtagaaccc gcgctggtcc gcgagcgtcg ccgaggcgcg
      361 gccggcgccg ccggccacca gcacgcgcat ggcatccatg agaccggcga aatgatcctc
      421 gggttccggc gcgtcctcgc ggcgcgcgag gccgaatgcg gcgaggtcgg cctggatgcg
      481 cacccgcgga tggtcgatcg ccggggctcc ggtatagaag ccggcgtaga gggagacctc
      541 ggccttgccc acgccgacga agagcgcctc gaactcttcc tgcgcggcat cggcatccat
      601 cgcgcacgac gcgtcgacca ggtcgcgcca cgcggcgccc aacgcgctcg tgcccgggat
      661 cggatcggcg ccggcgatgc ttccgaggag cgcccgatcg ggcccgctgt gcagcaggcg
      721 cgcgaagagc gcatagaaat cgccgcgggc cgcctcctcg ggcggcagcg tgcggtggag
      781 cgcgacgcgt gttgcgagcg catccatcag gaatcggctc ccagcctcag caccgaggcc
      841 tcgttcttgt tcgacatcat gtcgacgacg cggcagtcgg cgcacatctg cagccggcgc
      901 agcgccccgt cgctggagaa catcgaatgg cccgcgagcc ttcccagcat cgcctcgacc
      961 atctgcttcg tgccgaaggg ctttgcgcag ctcacgcagt ggaaaggctg ggcctcgttc
     1021 agcacgcggg cctcgcgcgc ctgcggagtg aggagaagcc gcggcgcgag actgatcgca
     1081 tcctcgggac aggtcgcttc gcacaggccg cattgcacgc aattgcgctc gaggaacttg
     1141 aggaggggct tgtccacgcc gtccatcagc gccgacacgg ggcatgcccc gacgcacgag
     1201 aggcacatcg tgcaccgggc cttgtccacc acgacctcgc cgaagggcga gcccgcggga
     1261 agggcgatca cgtcggcctt caccggcgcg agcttcgcga ggtgctcgac cgcgaattcg
     1321 atcgcggtgc gcttgtcgtt gcccaggagg aacgtggcgg aagaaggagg aacctgcgcc
     1381 ggcgaaagcc ttgcgaacgc ctgctcgagc cgaccggtgt cgctcgcttc gacgagagcg
     1441 aaatgcgtgc cgccgtaacc cagacccgca aggatcgctt ccgcgatcgc catctgcgag
     1501 cgcagcgcct cgcggtactc gacgtcttcg gagcccgcgc tgatcaccac cacctggttc
     1561 gcgccgaacg cgatcgcggg aagcagcagg tcgagaccga tcgcggacac atgccaggtt
     1621 tcgaggggca gcgcgcgcgc gggaaggccg gttcccgacg aggcccaggc ggcgaggagc
     1681 tcgcgcccgt catggccatt gtggaagacg atgcacgcgt cttccccgcc ggcatggcga
     1741 taggcggaaa ggag
//
LOCUS       DASVII010000214         5395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_46436, whole
            genome shotgun sequence.
ACCESSION   DASVII010000214 DASVII010000000
VERSION     DASVII010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46436"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1031
                     /locus_tag="VF309_02735"
     CDS             66..1031
                     /locus_tag="VF309_02735"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX7465529.1"
                     /translation="MKTSFLDFEQPIAELEQKIEELRFVQDDSAVDISAEIERLSKKS
                     RDLTKEIYAKLTAWQISKVARHPQRPYTLDYVNTICTDFEEMHGDRSFADDPAIVGGL
                     ARFNGQSVMVIGHQKGRDTNDKIFRNFGMPRPEGYRKALRLMKLAEKFAIPLITFIDT
                     PGAYPGIGAEERGQSEAIGKNLYAMAGLRVPVVACVIGEGGSGGALAIAVGDVTLMMQ
                     YSTYSVISPEGCAAILWKSADNAGEAADALAITSTRLKALGVVDKVVTEPLGGAHRDH
                     ADAAKSLKKAIQDSLRSLQDLSADALLERRQERWVNYGKFKELAE"
     gene            1069..2358
                     /gene="tilS"
                     /locus_tag="VF309_02740"
     CDS             1069..2358
                     /gene="tilS"
                     /locus_tag="VF309_02740"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7465530.1"
                     /translation="MGFSGGLDSTVLLDLMARHGRETGRAVSAVHVHHGLSPNADAWA
                     DSCAAFCAARGVPLTIERVRVDRDSAEGLEGAARAARYAVYARRDEPVVALAHHLDDQ
                     AETVLLQLLRGTGLKGVSAMPEMRALAGSGVRIYRPFLAFSRAALLEHAKAAGLGWIE
                     DESNVSSVHDRNYLRSELAPLLDARFPAWREALARFSRHAASADALLEELALEDGPDA
                     ASTEADVGAEPMFIHRPLADARRANALRSFLDRNALPMPATARLEEMARQVYDARDDA
                     QVRIEHGGVTLVRHRNRVYIESRPWAGEPWRVEWRGESHVELGGERGQVRFDAVVGKG
                     IDAARAAQGAWHFGSRSGGERIRLAPGRPTRTLKNLLQEQRFTPWQRQNFPCLFHEGR
                     LVWMSGIGIAAEYACPEGARGLDPRWIVARGARAVLK"
     gene            2395..2820
                     /gene="ndk"
                     /locus_tag="VF309_02745"
     CDS             2395..2820
                     /gene="ndk"
                     /locus_tag="VF309_02745"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7465531.1"
                     /translation="MALERTLSIIKPDAVGNNVIGEIYSRFEKAGLKIIAAKMVHLSR
                     NDAEGFYAVHKGRPFYKDLVEFMISGPVMIQVLEGPGAIGKNRDLMGATDPKKADKGT
                     IRADFARSIDANAVHGSDAPETAATEIAFFFPQSQVYSR"
     gene            2843..4033
                     /locus_tag="VF309_02750"
     CDS             2843..4033
                     /locus_tag="VF309_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7465532.1"
                     /translation="MSDKTNLLDFTLPAMTEWFGAQGEKPFRARQVFQWIHQRGVADF
                     GAMTDLAKSLREKLNAIAEVRAPAILSEHRSEDGTVKWLFDVGVGNGIETVYIPEDDR
                     GTLCVSSQVGCALDCKFCSTGRQGFNRDLGVAEIIGQVWVANRRLEEMEREGLASRLE
                     PRTSRLPAPNPDGAPRPGGEGESRSPRPVSNVVMMGMGEPLNNFEPVVAAMRIMLDDF
                     AYGLSRRRVTLSTSGVVPQIRKLAEALPVALAVSLHAPNDELRSRIVPLNKTYPIAEL
                     LAACREYLEVSPRDFITFEYVMLQGVNDAPDHAKELARLLKKVPSKVNLIPFNGFPDS
                     GFERSAVDRIKRFQQVLLDAGFIATIRRTRGEDIDAACGQLAGQVVNRMKKKTVRIHS
                     HPGH"
     gene            4033..4806
                     /gene="pilW"
                     /locus_tag="VF309_02755"
     CDS             4033..4806
                     /gene="pilW"
                     /locus_tag="VF309_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX7465533.1"
                     /translation="MRNALAAAILAAALAGCSSQSTVETKPVTDNRAADGHRRAEVHT
                     ALAGEYYARGNFTVALAEVRLALKDDSTYFPAYNMQGLVYMELHEDAQAREAFNQALL
                     MSPNNAEVLNNFGWFLCLRNDTQRGLEMIQRAATDNFYPTPEKAFLSAGLCLRRMGRT
                     QEAEDQLRRAVLIRPDMIGALFNLSVITFERGALKDSETYLLRYMRLTSSPSLEAITM
                     GVKIARGLNDSGAEQSYLQQLRRRFPDAPEALELLERRP"
     gene            4813..>5395
                     /locus_tag="VF309_02760"
     CDS             4813..>5395
                     /locus_tag="VF309_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7465534.1"
                     /translation="MESVESNADTASARTLGQTLASERERQGLSRSDAAQRLHMSAYQ
                     IEAIETGDYTRLPKGTFLRGFVRNYAKVLGLRAEDALALLPEHAPRDQKPGIVVPTQN
                     IRFDPLGDRLQNPYVKAASLAVVALVIGFAAMYWWLFIKPTPPGAAGRKATETQVAVA
                     SPAAQPESIAKAPAALAQPAKPEAAKIEPAKPEP"
BASE COUNT          907 a         1859 c         1824 g          805 t
ORIGIN      
        1 gcgattggcc atggaggcga attgtagctg ctatcattcg gccatcccat ggtttaccca
       61 gctacgtgaa aaccagcttc ctggatttcg agcagcccat cgcggaactc gaacagaaga
      121 tcgaggagct tcgcttcgtg caggacgact ccgcggtcga catctccgcg gagatcgagc
      181 gcctgtcgaa gaagagccgc gacctcacca aggagatcta cgcgaagctg accgcgtggc
      241 agatctcgaa ggtggcgcgc catccgcagc gcccctacac gctcgactac gtgaacacga
      301 tctgcaccga cttcgaggag atgcacggcg accgctcgtt cgccgacgat cccgcgatcg
      361 tgggcggcct cgcgcgcttc aacggccaga gcgtgatggt gatcggccac cagaaggggc
      421 gcgacaccaa cgacaagatc ttccgcaact tcggcatgcc gcgccccgag gggtaccgca
      481 aggcgctgcg cctcatgaag ctcgccgaga aattcgcgat cccgctcatc accttcatcg
      541 acacgccggg ggcgtacccg ggaatcggcg ccgaggagcg cggccagtcg gaggcgatcg
      601 gcaagaacct ctacgcgatg gcggggctgc gcgtgccggt cgtggcgtgc gtgatcggcg
      661 agggcggctc cggcggcgcg ctcgcgatcg cggtgggcga cgtgacgctc atgatgcagt
      721 actcgaccta ctcggtgatc tcccccgaag gctgcgcggc gatcctctgg aagagcgccg
      781 acaacgcggg cgaggcggcc gacgccctcg ccatcacctc gacgcggttg aaggcgctcg
      841 gtgtcgtcga caaggtggtg accgagcccc tgggcggcgc ccatcgcgac cacgcggatg
      901 ctgcgaagag cctgaagaag gcgatccagg acagcctgag aagcctccag gacctctcgg
      961 ccgacgcgct cctcgagcgg cgccaggaac gctgggtcaa ctatgggaag ttcaaggaac
     1021 tcgccgagta gcggcttcgc gagcctgccc ccgcgcgccg cggtctgcgt ggggttctca
     1081 ggcggcctcg attccacggt gctgctcgac ctgatggcgc gccacggccg cgaaacggga
     1141 cgggccgttt ccgcggtgca cgtccaccac ggactgagcc ccaacgcgga tgcctgggcc
     1201 gattcctgcg cggcgttctg cgccgcgcgc ggcgtgccgc tcacgatcga acgcgtgcgc
     1261 gtcgatcgcg attccgccga aggcctcgag ggcgcagccc gcgccgcgcg ctacgccgtg
     1321 tacgcgagac gcgacgagcc ggtggtcgcg ctcgcccacc atctcgacga ccaggcggaa
     1381 acggtgttgc tgcagctgct gcgcgggacg gggctgaagg gcgtctccgc gatgccggag
     1441 atgcgcgcgc ttgcggggtc cggcgtgcgg atctaccgcc cgttcctcgc gttttcgcga
     1501 gcggcgctgc tcgagcacgc gaaggccgcg ggcctcgggt ggatcgagga cgagtcgaac
     1561 gtctcgagcg tgcacgatcg caactatttg cgaagcgagc tcgcgccgct cctcgacgcg
     1621 cgttttcccg cgtggcgcga ggcgcttgcg cgcttctcgc gccacgcggc gagcgccgac
     1681 gcattgctcg aggagctcgc gctcgaggac ggcccggacg cagcctcgac ggaagccgac
     1741 gtcggcgccg agccgatgtt catccaccgc cccctggccg acgcgcgccg cgccaacgcg
     1801 ttgaggtcgt tcctcgatcg caacgcgctg ccgatgcccg ccaccgcgcg cctcgaggag
     1861 atggcgcgcc aggtctacga cgcgcgcgac gacgcgcagg tgcggatcga gcatgggggc
     1921 gtcacgctcg tgcgccatcg caaccgcgtt tacatcgaaa gccgcccgtg ggccggcgag
     1981 ccgtggcggg tggagtggcg gggcgaatcg cacgtcgagc tcggaggcga gcgcgggcag
     2041 gtccgcttcg acgcggtcgt gggcaagggc atcgatgccg cgcgcgcggc gcagggcgca
     2101 tggcacttcg gatcccgcag cgggggcgag cgcattcgcc tcgcgccggg ccggcccacg
     2161 cgcaccttga agaacctgct ccaggagcag cggtttacac cctggcagcg gcaaaatttt
     2221 ccgtgccttt tccacgaggg ccggctggtt tggatgagcg gcatcggcat cgccgccgaa
     2281 tacgcgtgcc cggagggcgc gagggggctc gatccgcgct ggattgtggc gcgcggcgcg
     2341 cgcgctgtgt taaaatgaaa agctaaactt ttcatcaatt tagcggagat tttcatggcg
     2401 ttggagcgca ccctatcgat catcaaaccc gacgccgtgg gcaacaacgt gatcggcgag
     2461 atctactccc gattcgagaa ggcgggcctg aagatcatcg cggcgaagat ggtccacctg
     2521 tcgcgcaacg acgccgaggg cttctatgcc gttcacaagg gccgcccgtt ctacaaggac
     2581 ctggtcgaat tcatgatctc gggcccggtg atgatccagg tgctcgaggg accgggcgcc
     2641 attgggaaaa atcgcgacct catgggcgca accgatccta agaaggccga caagggcacc
     2701 attcgcgccg atttcgcgcg gtcgatcgac gcgaacgcgg tgcacggctc ggatgcgccg
     2761 gagacggccg ccaccgagat cgcgttcttc tttccgcaga gccaggtcta cagcaggtag
     2821 ggcgcgagcc ccgaggcgcg tgatgagcga caagaccaac cttctcgatt tcaccctgcc
     2881 cgcgatgacc gagtggttcg gcgcgcaggg ggagaagccg tttcgcgcgc gccaggtgtt
     2941 ccagtggatc catcagcgcg gcgtcgccga tttcggcgcg atgacggacc tcgcgaaatc
     3001 gctgcgcgag aagctgaacg cgatcgccga agtgcgcgcg cccgcgatcc tctcggagca
     3061 ccgctccgag gacggcaccg tgaagtggct cttcgacgtc ggcgtgggca acggcatcga
     3121 gaccgtgtac atccccgagg acgatcgcgg cacgctctgc gtgtcttcgc aggtcggctg
     3181 cgcgctcgac tgcaagttct gctcgacggg ccgccaggga ttcaatcgcg acctgggcgt
     3241 cgcggagatc atcggccagg tgtgggtcgc caaccgccgc ctcgaggaga tggagcgcga
     3301 gggactggct tcgcgcctcg agccgcgcac ctcgcgcctg cccgccccga acccggatgg
     3361 cgcgccgcgc ccgggcgggg aaggcgaatc gcgctcgccg cggcccgtga gcaacgtcgt
     3421 gatgatgggc atgggcgagc cgctgaacaa tttcgagccc gtggtcgccg cgatgaggat
     3481 catgctcgac gatttcgcct acgggctttc gcgccgccgc gtgacgctct cgacttcggg
     3541 cgtcgtgccg cagatccgca agctcgccga ggccttgccg gtggcgctcg cagtaagcct
     3601 gcatgcgccc aacgacgagc tgcgcagccg catcgtgccg ctgaacaaga cctatccgat
     3661 cgccgagctc ctcgccgcgt gccgcgagta tctcgaggtc tcgccgcgcg acttcatcac
     3721 cttcgagtac gtgatgctcc agggcgtgaa cgacgcgccc gaccacgcga aggagctcgc
     3781 gcgcctgctg aagaaggtgc cgagcaaggt gaacctcatc ccgttcaacg gcttccccga
     3841 ttcgggcttc gagcgctccg ccgtggatcg catcaagcgc ttccagcagg tcctgctcga
     3901 cgccggcttc atcgcgacca ttcgccgcac gcgcggcgag gacatcgacg cggcgtgcgg
     3961 ccagctcgcc ggccaggtgg tgaaccgcat gaagaagaag accgtccgca tccactcaca
     4021 tccgggacac tgatgcgcaa cgcactcgcc gccgcaatcc tcgccgccgc gctcgccggc
     4081 tgctcctccc agagcacggt cgagacgaag cccgtcaccg acaacagggc ggccgacggc
     4141 catcgccgcg ccgaagtgca cacggccctc gccggcgagt actacgcgcg cggcaatttc
     4201 acggtggcgc tcgccgaggt gcgcctcgcg ctgaaggacg attcgaccta ctttcccgcc
     4261 tacaacatgc agggcctcgt ctacatggag ctgcacgagg acgcgcaggc gcgcgaggcg
     4321 ttcaaccagg cgctgctgat gtcgcccaac aacgccgagg tgctcaacaa cttcggctgg
     4381 ttcctgtgcc tgcgcaacga cacgcagcgc gggctcgaga tgatccagcg cgccgccacc
     4441 gacaatttct accccacgcc ggagaaggca ttcctctcgg cgggcctttg cctgcgccgc
     4501 atggggcgca cgcaggaagc cgaggaccag ctgcgccgcg cggtgctcat ccggcccgac
     4561 atgatcggcg cgctcttcaa cctttcggtg atcacgttcg agcgcggagc gctcaaggat
     4621 tcggagacgt acctgcttcg ctacatgcgc ctcaccagct cgccgagcct cgaggcgatc
     4681 acgatgggcg tgaagatcgc ccggggcctg aacgacagcg gcgccgagca aagctacctg
     4741 cagcagctgc gccggcgctt ccccgatgcg ccggaagcgc tcgagcttct cgagaggcgc
     4801 ccgtaggttg ccgtggaatc cgtcgaatcc aacgccgaca ccgcctcggc ccgcacgctg
     4861 ggacagacgc tcgcgtcgga gcgtgagcgc caggggctgt cgcgctccga cgccgcgcag
     4921 cgccttcaca tgagcgccta ccagatcgag gcgatcgaga ccggcgacta cacgcgcctg
     4981 ccgaagggca cgttcctgcg cggcttcgtt cgcaactacg cgaaggtcct cgggcttcgc
     5041 gcggaagacg cgctcgcgct ccttcccgag catgcgccgc gcgaccagaa gcccggcatc
     5101 gtggtgccga cgcagaacat ccgcttcgat ccgctcggcg atcgcctgca gaatccctac
     5161 gtgaaggcgg cgagcctcgc ggtggtcgcg ctggtgatcg ggttcgcggc gatgtactgg
     5221 tggctcttca tcaagcccac gccgcccggc gccgcggggc gcaaggccac cgagacccag
     5281 gtcgccgtgg cgtccccggc ggcgcagccg gaatcgatcg cgaaggcgcc cgcggcgctg
     5341 gcccagcccg cgaagcccga ggccgcgaag atcgagcccg cgaagcccga gccgc
//
LOCUS       DASVII010000215         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_189819,
            whole genome shotgun sequence.
ACCESSION   DASVII010000215 DASVII010000000
VERSION     DASVII010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189819"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VF309_02765"
     CDS             <1..773
                     /locus_tag="VF309_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7465535.1"
                     /translation="QFEATSKDGTKIPYFVVMREGTKLDGSNPTILYGYGGYEISMLP
                     SYSGTIGAAWLEKGGIWVLANIRGGGEFGPAWHTTAIREGRHKTHDDFIAVAEDVIKR
                     GITSSKHLGIMGGSQGGLLVGAAFTQRPDLFKAVVCQVPLLDMRRFNKLLAGASWQGE
                     YGNPDVPADWEFISKYSPYQNVVKEKKYPPVLFYTSPRDDRVHPGHARKMVAKMEEQG
                     HDVLYYENTEGGHAAGSTPAQQAFMWALTYTYFLNELR"
     gene            complement(785..>1669)
                     /locus_tag="VF309_02770"
     CDS             complement(785..>1669)
                     /locus_tag="VF309_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEX7465536.1"
                     /translation="ADLVLEITLGLVWSQLSRKHRDVPRFAVIAYGKLGGKELGYGSD
                     LDIIFLYDPAAAREPPEKLARIAQRMVTWLTSHTAAGVLYDADLRLRPDGASGLMVSS
                     LESFRDYQAKRAWTWEHQALTRARFSAGDAALGRRFEAARDEILATPRDRARLFEEIQ
                     AMRRKMRAENRSEAQELKQIEGGIIDLEFAVQALVLAEGPAHPGLRENKGNHTLLHRA
                     GDLGLVDRTIASDAAGAYLAMRKRTHEAARLADELRARGHQVSVEPLMSGAGYIRRAG
                     NGWIGAADPRRGGNASGE"
BASE COUNT          267 a          572 c          567 g          263 t
ORIGIN      
        1 gccagttcga ggcgacgtcg aaggacggca cgaagatccc ctacttcgtg gtgatgcgcg
       61 agggcacgaa gctcgacggc tccaatccca cgatcctcta cggatacggc ggctacgaga
      121 tctcgatgct gccgagctac agcggcacca tcggagcggc gtggctcgag aagggcggca
      181 tctgggtcct cgccaacatt cgcggcggcg gcgagttcgg tcccgcctgg cataccaccg
      241 cgattcgcga agggcgccac aagacccacg atgatttcat cgcggtggcc gaggacgtca
      301 tcaagcgcgg catcacgtct tcgaagcacc tcggcatcat gggcggcagc cagggcggtc
      361 tgctcgtggg cgccgcgttc acccagcgcc ccgatctctt caaggcggtc gtgtgccagg
      421 tgccgctcct cgacatgcgc cgtttcaaca agcttctcgc gggcgcctca tggcagggcg
      481 agtatggcaa tcccgacgtt cccgcggact gggagttcat ctcgaagtat tcgccctacc
      541 agaacgtcgt gaaggagaag aagtatccgc ccgtgctctt ctacacgtcg ccccgcgacg
      601 atcgcgtgca cccgggccat gcgcgcaaga tggtcgcgaa gatggaagag caagggcacg
      661 acgtcctgta ctacgagaac accgaaggcg gccacgccgc gggctcgacg cccgcgcagc
      721 aggccttcat gtgggccctc acgtatacgt acttcctcaa cgagcttcgc tgacgccaat
      781 ttcatcactc gcctgacgcg ttgccgccgc ggcgaggatc ggcggcgccg atccagccgt
      841 tgccggcgcg cctgatgtag cccgcgccgg acatgagcgg ctcgaccgag acctggtggc
      901 cgcgcgcgcg cagctcgtcg gcgaggcgcg cggcctcgtg cgtgcgcttg cgcatcgcga
      961 ggtaggcgcc cgcggcgtcg gaggcgatcg tcctgtcgac aagtcccagg tcgcccgcgc
     1021 gatgaagcag cgtgtggttg cccttgttct cgcgaagccc gggatgcgcg gggccctcgg
     1081 cgaggaccag cgcctgcacc gcgaactcga ggtcgatgat gccgccctcg atctgcttca
     1141 gctcctgcgc ctcggagcgg ttctccgcgc gcatcttgcg gcgcatcgcc tggatctcct
     1201 cgaagagcct ggcgcgatcg cggggcgtgg cgaggatctc gtcgcgtgcc gcctcgaagc
     1261 gccggccgag cgcggcgtcg cccgccgaaa agcgcgcgcg cgtgagcgcc tggtgctccc
     1321 acgtccacgc gcgcttcgcc tggtagtcgc gaaacgactc gagcgagctc accatgaggc
     1381 ccgaggcgcc gtcggggcgc agccgcaggt ccgcgtcgta gagcacgccc gccgcggtgt
     1441 ggctggtgag ccacgtgacc atgcgctgcg cgatcctcgc gagcttctcg ggcggctcgc
     1501 gcgcggccgc gggatcgtag aggaagatga tgtcgaggtc cgagccgtag ccgagctcct
     1561 tgccgccgag cttgccgtag gcgatcaccg cgaagcgcgg aacgtcgcgg tgtttgcggc
     1621 tcaactgcga ccacaccagc ccgagcgtga tctcgagaac cagatcggc
//
LOCUS       DASVII010000216         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_46509, whole
            genome shotgun sequence.
ACCESSION   DASVII010000216 DASVII010000000
VERSION     DASVII010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46509"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VF309_02775"
     CDS             <1..840
                     /locus_tag="VF309_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7465537.1"
                     /translation="TEVTGDAAPVEEDAALEAGEERDAGEVEAGLSRHCPKDMVNVAG
                     RLCIDRYEDSLVDDVTGERLSPHYPPEPGVPLRMFAKWQEERLNVGPPWARAMPLPIV
                     PEIEQADDFRPRAVSIRGVYPQGYISGRVAGEACARAKKRLCTHDEWAFACRGEKQTK
                     YPYGSEYQPGVCNVFRSSHPAQRLHDNASAGHSDPRLGLVKGEDGPLLRTTGSTKECV
                     SRWGKDFIYDMVGNEDEWIDDPDGTFVGGFFSRSTKEGCDSIVTVHPITHWDYSIGLR
                     CCR"
     gene            845..>1637
                     /locus_tag="VF309_02780"
     CDS             845..>1637
                     /locus_tag="VF309_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7465538.1"
                     /translation="MNEIRWGIIGCGDVTEVKSGPGLQRAPGSRLVAVMRRNGALAED
                     YARRHGVEAWYDDARALIDDPRVDAVYVATPPGSHLEYALLACAARKPVYVEKPMARC
                     HAECIEMVRAFSEARVPLFVAYYRRALPRFQKARDLVISGKLGAITGVSYHLAQPFHR
                     KVDARDVPWRVRAEEAGGGLFVDVGCHTLDILDYIFGPLENVNGTAANVRSPYDVEDS
                     VSMRFALPSGALGVAQWNFASAAETDEIVIQGELGEVRLSTFGREP"
BASE COUNT          285 a          522 c          554 g          276 t
ORIGIN      
        1 acggaggtga cgggcgacgc cgcccccgtg gaggaggatg ccgcgctcga agcaggcgaa
       61 gaacgcgacg ccggcgaagt cgaggcgggt ttgagccgcc attgcccgaa ggacatggtc
      121 aacgtcgccg gccgcctttg catcgatcgc tacgaggatt cgctggtcga cgacgtcacg
      181 ggagaacgct tgtcgcccca ctacccgccc gagcccggcg tgcccttgcg catgttcgcc
      241 aagtggcagg aagagcgcct caacgtggga ccgccgtggg cgcgcgcgat gccgttgccg
      301 attgttccgg agatcgagca agcagacgat ttcaggccgc gggcagtgtc gattcgcggg
      361 gtgtatcccc aggggtacat cagcgggcgt gtggccggcg aagcctgcgc gcgcgccaag
      421 aagcgcctct gcacgcacga tgagtgggcg ttcgcctgtc gcggcgagaa acaaaccaag
      481 tacccctatg gcagcgagta ccagcccggc gtctgcaacg tctttcgctc gtcgcacccc
      541 gcccagaggc tgcacgacaa cgcctcggcc ggacattccg atccgcgcct tggcctcgtg
      601 aagggtgaag acggaccatt gctccgaacg accggctcga ccaaggagtg cgtaagccgg
      661 tggggcaagg acttcatcta cgatatggtg ggcaacgagg acgaatggat cgacgatccc
      721 gatggaacct tcgtgggagg attcttctcg cggtcgacca aggaagggtg cgactcgatc
      781 gtgaccgttc accccatcac acactgggac tactcgattg gtctccgatg ttgccggtga
      841 gctcatgaac gaaatccgct ggggcatcat cggttgtggc gacgtcacgg aagtgaagag
      901 cggtcctggt ttgcagcgtg cgccggggag ccgcctcgtc gccgtcatgc gccgcaatgg
      961 cgcgctcgcc gaggactacg ctcggcgaca cggcgtcgag gcctggtacg acgacgcccg
     1021 ggcactcatc gacgaccctc gtgtcgacgc cgtttacgtc gcgacgccgc ccggatcgca
     1081 cctcgagtac gccctcctgg cttgcgcggc aagaaagccc gtctacgtcg agaaaccgat
     1141 ggctcgttgc cacgccgagt gcatcgagat ggtgcgcgcg ttctccgagg cgcgggtacc
     1201 gctcttcgtc gcctactacc gtcgggcgct gccgagattc cagaaggcgc gcgatctcgt
     1261 catatcgggc aagctgggag ccatcacggg cgtgagctac cacctggcgc agccgttcca
     1321 ccgcaaggtc gatgcgcgcg atgttccgtg gcgcgtgcgc gccgaggaag ctgggggcgg
     1381 gctgttcgtc gatgtcggct gtcacacgct cgacattctc gactacatct tcggtccgct
     1441 cgagaacgtg aacggaaccg ccgccaacgt gcgctcgccc tacgacgtcg aggattcggt
     1501 cagcatgcgg ttcgcgctcc cctcgggagc tctcggggtc gcgcagtgga actttgcgag
     1561 cgctgccgaa accgatgaga tcgtgatcca gggcgagctg ggcgaggtgc gcctttcgac
     1621 gttcggtcgc gagcccg
//
LOCUS       DASVII010000217         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_334164,
            whole genome shotgun sequence.
ACCESSION   DASVII010000217 DASVII010000000
VERSION     DASVII010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334164"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..958)
                     /locus_tag="VF309_02785"
     CDS             complement(26..958)
                     /locus_tag="VF309_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7465539.1"
                     /translation="MALAAAPALAQLSWPSKPVTIVLPYPPGGSNDTFARELGKKLSE
                     AWKIPVIVDNRPGAGGSIGAALVSKASPDGYTLCLLSSSFTTNAAVQPSLPFDPIAGF
                     TPVAMVAKGPMILTVSNKLPAKTTLELFDLARRNPGKLNYASSGQGSTNHFATELLME
                     AAHIKMTHVPYKGMAPAVTDLIGGHVDVLVASAPSIYQHVKAGSVRGLGVTSKGRSPV
                     VPDLQPLATMGVPNYSFELWWGILAPPGTSPDIAIQVNIDVNRILASPEMKEVLLREG
                     AEPAPMSVAQFAATIRNEIAGWKKVAASAGIKPE"
     gene            complement(1045..1926)
                     /locus_tag="VF309_02790"
     CDS             complement(1045..1926)
                     /locus_tag="VF309_02790"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7465540.1"
                     /translation="MRSKLFVPGSRPELFAKAMAGEADGLSFDLEDAVDESMKDEARR
                     ELAKFLRTLAPGHGKVIIVRVNGLATPHFAADIEAVAGAGVDLVNLPMIESPDDVREC
                     AEAIERAEREASVGPIGILANIETPKAYRLAAEIASASPRVKGLQAGWGDLIEPLDID
                     RYNAPMIESLLLHIRIAAGEGGVWAYDGAYANFKDPEGYKREAEAARRLGYLGKSCIH
                     PSQVALANAAFRPSDAEIAHSLRVVEAAREAAAKGIGAFTVNGRMVDAPFVRRAEAVL
                     ALAEKLRLIAPDERTVQ"
     gene            complement(1931..>2357)
                     /locus_tag="VF309_02795"
     CDS             complement(1931..>2357)
                     /locus_tag="VF309_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7465541.1"
                     /translation="KGKFALDTVIIDACAMKLVLNPWQFDVLVSTNLFGDILSDLNAG
                     LVGGLGMAPAGNIGADAAIFEAVHGSAPDIAGKGVANPLALMLSAAMMLDHVGHQDKA
                     KRVRDAIDAVLLKDKVRTGDLGGKASTKQFTKAVASRLG"
BASE COUNT          362 a          790 c          804 g          401 t
ORIGIN      
        1 gtagtcgcgc ttcttcagca cgcgctcatt ccggcttgat gcccgcgctc gccgcgacct
       61 tcttccatcc ggcgatctcg ttcctgatgg tcgccgcgaa ttgcgcgacg gacatcggcg
      121 cgggctccgc gccctcgcgc aggagcactt ccttcatctc cgggctggcg aggatccggt
      181 tcacgtcgat gttgacctgg atcgcgatgt cgggcgacgt gcccggcggc gcgaggattc
      241 cccaccagag ctcgaagctg tagttcggca cgcccatcgt cgcgaggggc tgcaggtcgg
      301 gaacgacggg cgagcggccc ttgctcgtca cgccgaggcc gcgcacgctg cccgccttca
      361 cgtgctggta gatcgagggc gcgctcgcga cgagcacgtc gacgtggcct ccgatgaggt
      421 cggtcaccgc gggcgccatc cccttgtagg gaacgtgcgt catcttgatg tgcgccgcct
      481 ccatcagcag ctcggtcgcg aaatgattgg tgctgccctg ccccgaggag gcgtagttga
      541 gcttgcccgg gttgcgccgc gcgaggtcga agagctcgag tgtcgtcttc gcgggaagct
      601 tgttcgagac ggtgaggatc atcggcccct tggcgaccat cgcgaccggc gtgaagcccg
      661 cgatgggatc gaagggaaga ctcggctgga cggccgcgtt ggtggtgaag gacgacgaga
      721 gaaggcacag cgtatagcca tcgggcgacg ccttcgacac cagggccgcg ccgatcgagc
      781 cgcccgcacc cggacggttg tcgacgatca ccgggatctt ccacgcctcg gagagcttct
      841 tcccgagctc gcgcgcgaaa gtgtcgttcg atccgcccgg gggatacgga aggacgatcg
      901 tgaccggctt cgacggccac gagagctgcg cgagcgcggg cgccgccgca agcgccaagg
      961 ccgcgaaaat tggtgtcagg tacgcatttc ggcagtgagc accggcgagc cggtgcaacc
     1021 gaaatgcgta cctgacacca attttcattg aaccgtccgc tcgtcgggag caatcagccg
     1081 caatttctcg gcgagggcga gcaccgcctc ggcgcggcgc acgaagggcg cgtcgaccat
     1141 ccggccgttc acggtgaagg cgccgatgcc cttcgcggcg gcttcccggg ccgcctcgac
     1201 cacccgcagc gaatgcgcga tctccgcgtc gctcgggcgg aacgcggcat tggcgagcgc
     1261 cacctggctc ggatggatgc agctcttgcc gaggtagccc aggcgccgcg cggcttcggc
     1321 ctcgcgcttg tagccttccg gatccttgaa gttggcgtag gcgccgtcgt aggcccacac
     1381 gccgccctct cccgcggcga tgcggatgtg caggaggagc gactcgatca tcggcgcatt
     1441 gtagcgatcg atgtcgagcg gctcgatgag gtcgccccaa ccggcctgca gccccttcac
     1501 gcgtgggctc gccgaggcga tctcggcggc gaggcggtac gccttgggag tctcgatgtt
     1561 ggcgaggatg ccgatgggtc cgacgctcgc ctctcgctcg gccctttcga tcgcctccgc
     1621 gcactcgcga acgtcatcgg gcgattcgat catgggcagg ttcacgagat cgacgccagc
     1681 gccggcgacg gcttcgatgt cggcggcgaa atgcggcgtc gccaatccat tgacgcgcac
     1741 gatgatcacc ttgccgtggc ccggggccag cgtgcgaaga aatttcgcga gctcgcgccg
     1801 cgcctcgtcc ttcatcgatt cgtcgacggc gtcctcgaga tcgaaggaca atccgtcggc
     1861 ctcgcccgcc atcgccttcg cgaagagctc cggccgcgag ccgggcacga agagcttcga
     1921 gcgcatgggc tcagccgagg cggctggcga ccgccttcgt gaactgcttg gtgctcgcct
     1981 tgccgccgag gtcgccggtg cgcaccttgt ccttcagcag caccgcgtcg atcgcatcgc
     2041 gcacgcgctt cgccttgtcc tggtggccga cgtggtcgag catcatcgcc gcggagagca
     2101 tcaacgcgag cgggttggcc acgcccttgc cggcgatgtc gggcgccgac ccgtgaaccg
     2161 cctcgaagat cgcggcgtcg gcgccgatgt tgccggcggg cgccatcccc aggccgccca
     2221 cgaggcccgc gttcaggtcc gagaggatgt cgccgaagag attggtggat accaggacgt
     2281 cgaattgcca ggggttgagc accagcttca tggcgcatgc atcgatgatg accgtatcca
     2341 gcgcgaactt gcccttg
//
LOCUS       DASVII010000218         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_334213,
            whole genome shotgun sequence.
ACCESSION   DASVII010000218 DASVII010000000
VERSION     DASVII010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334213"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /gene="livM"
                     /locus_tag="VF309_02800"
     CDS             <1..1254
                     /gene="livM"
                     /locus_tag="VF309_02800"
                     /inference="COORDINATES: protein motif:HMM:NF008450.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEX7465542.1"
                     /translation="PQTTPLAKADDAAAASQLVPALRTAIVSGLVAFGLCFPIISYHA
                     ESNINNELILTGRWPLTFIITAIVFVFVFLRQMANADWRIWFDRFGSIVAGGMGGALV
                     QAAREGEGVAPPSRARVAFGRYVGPFMLGLVIVFPLIMLGLLGPAGSLKWINNYGVQI
                     MIYVMLGWGLNIVVGLAGLLDLGSVAFYAVGAYSYALLSTTFGLSFWICLPLAGLLAA
                     MWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWVDLTNGYAGISGIPHVSFFGIP
                     FNDEDNGFAAIFHIPYSPIYRTIFLFYLILALALLTNFVTLRLRRLPIGRAWEALRED
                     EIACRSLGINTTTTKLTAFALGAMFGGFAGSFFSVRQGFVSPESFTFIESATILAIVV
                     LGGMGSQLGVAIAAAVMIGVPELMR"
     gene            1309..1446
                     /locus_tag="VF309_02805"
     CDS             1309..1446
                     /locus_tag="VF309_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465543.1"
                     /translation="MLLFGAAMVIMMIWKPRGLVSTRAPSVFLKEQKAVSGDLVKEGH
                     G"
BASE COUNT          231 a          563 c          474 g          336 t
ORIGIN      
        1 ccacagacca cgccgctcgc caaggccgac gacgcggcgg ccgcctcgca actcgtcccc
       61 gcgcttcgca ccgcgatcgt ctcggggctg gtcgccttcg ggctctgctt tccgatcatc
      121 agctatcacg ccgaatcgaa catcaacaac gagctcatcc tcaccgggcg ctggcccctc
      181 accttcatca tcacggcgat cgtctttgtc ttcgtcttcc tcaggcagat ggcgaacgcc
      241 gactggcgca tctggttcga ccgcttcggg tcgatcgtcg ccggcggaat gggcggggcg
      301 ctggttcagg cggcgcgcga aggcgaaggc gtcgcgccgc cgtcgcgggc gcgcgtcgcg
      361 ttcgggcgct atgtcggccc gttcatgctg ggcctcgtca tcgtgtttcc cctcatcatg
      421 ctgggcctgc tggggcccgc cggctcgctg aagtggatca acaattacgg cgtccagatc
      481 atgatctacg tcatgctggg ctggggactg aacatcgtgg tcggcctcgc cggcctgctc
      541 gaccttggct ctgtcgcctt ctacgccgtc ggcgcctatt cctatgcgct gctctcgacg
      601 accttcggcc tctcgttctg gatttgcctg ccgctcgccg ggctattggc ggcgatgtgg
      661 ggcgtcatac tcgggttccc ggtcctgagg ctgcgcggcg actatctcgc catcgtgacg
      721 ctcgccttcg gcgagatcat ccgcctcgtc ctcatcaact gggtcgacct gaccaacggc
      781 tacgccggca tctccggcat cccgcacgtc agctttttcg gcattccctt caacgacgag
      841 gacaacggct tcgccgcgat cttccatatc ccctattcgc cgatctaccg cacgatcttt
      901 ctcttctatt tgatcctggc gctggcgctc ttgacgaatt tcgtgaccct gcgcctgcgg
      961 cgcctgccga tcgggcgcgc ctgggaggcg ctgcgcgagg atgagatcgc ctgccgctcg
     1021 ctgggcatca acaccaccac cacgaagctc accgcctttg cgctcggcgc gatgttcggc
     1081 ggcttcgccg gctccttctt ttccgtgcgc cagggcttcg tcagccccga aagcttcacc
     1141 ttcatcgaat cggcgacgat tctcgccatc gtcgtgctcg gcggcatggg ctcgcagctc
     1201 ggcgtcgcca tcgccgccgc ggtgatgatc ggggtgcccg aactgatgcg ctaactcgac
     1261 tggctcaagg cgattttcgg ccaggacttc gatccgacgc aataccggat gctgttgttc
     1321 ggcgcagcga tggtcatcat gatgatctgg aagccgcgcg gcctcgtgtc gacgcgagcc
     1381 ccatcggtct ttctcaagga gcagaaggcc gtgtcgggcg atctcgtcaa agagggccac
     1441 ggctaatgaa ctggctgacc agcccgctgc tcgccgtcga gcacctgacc atgcgcttcg
     1501 gcgggctgat cgcgatcgac gatctctcct tcagcgccaa ccagggcgag atcaccgcca
     1561 tcatcggccc taacggcgcc ggcaagacca cggtcttcaa ctgc
//
LOCUS       DASVII010000219         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_154042,
            whole genome shotgun sequence.
ACCESSION   DASVII010000219 DASVII010000000
VERSION     DASVII010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154042"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2224)
                     /locus_tag="VF309_02810"
     CDS             complement(<1..>2224)
                     /locus_tag="VF309_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7465544.1"
                     /translation="FISIDDMEGPEWNYAAMSPAKHELATALLYRLRKRFAPGGLLHF
                     GQGKWYPGEPLPRWALSCFWRKDGVALWEREDEDARPPEGGKNTEEDARRLATELAQA
                     LELSPEYVLPAYEDPWRTLRDEANLPVNVDLFAEDTASEPARRRLAGKIAGGLGNPVG
                     YVLPLKPVARGKASHPTTWQSSPWPLRREKVFLIEGDSPLGYRLPLASLPEVLPEDDE
                     PSIPQDPFDRRDALGKRKAAKSPLGAKHAKPKALPPGAPREVIKRALAVEVRKGALHV
                     FLPPVDILEDFISLIGAIEATAQALGVRVRLEGYPPPDDPRLGRFAVTPDPGVIEVNI
                     HPAGNWEELKHHVETLYEEARATRLGTEKFMLDGRHTGTGGGNHVTLGGATAPDSPLL
                     RRPDLLQSLVTYWQNHPALSYLFSGMFIGPTSQSPRVDEARSDTLYELEIAFEQLERE
                     FKPGEPNEKPWLVDRILRNFLVDLTGNTHRAEFSIDKLYAPGTATGRLGLLEFRAFEM
                     PPHARMSLLQILLLRALVARFWSTPYREKLVHWGTRLHDEFMLPHFVAQDMRDVVDDL
                     KAAGYPFQFEWFAPFHEFRFPRYGSVSYDGIGVELRQAIEPWHVLGEQVAQTGTSRYV
                     DSSIERLQVKAMRFNEERHAITVNGRYVPMTSTGRAGEFVAGVRYRAWAPPSALHPTI
                     GIHAPLVFDLVDLWNQRSLGGCTYHVSHPGGRNYSTFPVNALEAEARRVARFQAMGHT
                     P"
BASE COUNT          338 a          758 c          779 g          349 t
ORIGIN      
        1 ctggagtatg tcccatcgcc tggaagcgcg cgacgcggcg cgcctcggcc tcgagcgcgt
       61 tcaccggaaa ggtcgagtag ttgcgcccgc ccggatgcga cacgtgatag gtgcagccgc
      121 cgagggagcg ctggttccat agatcgacga ggtcgaacac cagcggcgcg tggatgccga
      181 tggtgggatg cagcgccgaa ggcggcgccc acgcgcggta gcgcacgccg gcgacgaact
      241 ctcccgcgcg ccccgtcgag gtcatcggca cgtagcgccc gttcaccgtg atcgcgtgcc
      301 gctcctcgtt gaagcgcatc gccttgacct gcaggcgctc tatcgacgaa tcgacgtagc
      361 gcgaggttcc cgtctgcgcg acctgctcgc ccagcacgtg ccacggctcg atcgcctgcc
      421 tcagctcgac cccgatgccg tcgtaggaga ccgatccgta gcgcggaaag cggaactcgt
      481 ggaacggcgc gaaccactcg aactggaagg gatagcccgc ggccttgaga tcgtcgacca
      541 cgtcgcgcat gtcctgggcg acgaagtggg gcagcatgaa ctcgtcgtga agccgcgtgc
      601 cccagtgcac cagcttctcg cgatacggcg tgctccagaa gcgcgcgacc agcgcgcgca
      661 gcaacaggat ctgcagcagg ctcatgcggg cgtggggcgg catctcgaag gcgcggaatt
      721 ccaggagccc caggcgcccg gttgccgtgc ctggagcgta gagcttgtcg atcgagaatt
      781 ccgcgcggtg ggtattgccc gtgaggtcca ccaggaaatt gcgcaggatg cggtcgacga
      841 gccacggctt ctcgttcggc tcgcccggct tgaactcccg ctcgagctgc tcgaaggcga
      901 tctcgagctc gtagagcgtg tcgctgcgcg cctcgtccac gcgcggcgac tggctcgtcg
      961 gcccgatgaa cattcccgag aagagatacg aaagcgcggg atggttctgc cagtaggtga
     1021 cgaggctttg caggaggtcg gggcggcgca ggagcgggct gtcgggcgcg gtggccccgc
     1081 cgagcgtcac gtggttgccg ccgccggtgc ccgtatggcg gccatcgagc atgaacttct
     1141 cggtgccgag gcgcgtcgcg cgcgcttcct cgtagagcgt ctcgacgtga tgcttgagct
     1201 cctcccagtt gccggccgga tggatgttca cctcgatcac gccgggatcg ggcgtcaccg
     1261 cgaagcgccc gaggcgcgga tcgtccggcg gcggatagcc ttcgagccgc acgcgcacgc
     1321 cgagcgcctg cgcggtcgcc tcgatcgcgc cgatgagcga gatgaagtcc tcgaggatgt
     1381 cgacgggcgg aaggaagacg tggagcgcgc ccttgcgcac ctcgacggcg agcgcgcgct
     1441 tgatcacctc gcgcggcgct ccgggcggaa gcgccttcgg cttcgcgtgc ttcgccccca
     1501 gcggcgactt cgcggccttc ctcttcccca gcgcgtcgcg gcgatcgaac ggatcctgcg
     1561 gaatgctcgg ctcgtcatcc tcgggaagga cctcggggag cgaggcaagc ggcaggcgat
     1621 agccgagcgg cgaatcgccc tcgatgagga acactttctc gcggcgcaac ggccagggcg
     1681 agctttgcca ggtcgtcgga tggctcgcct tgccgcgcgc gacgggcttc aacggcagca
     1741 cgtagcccac ggggttgccg agcccccccg cgatcttccc ggcgaggcgg cggcgcgcgg
     1801 gctcgctcgc cgtgtcctcc gcgaagagat ccacgttcac gggcaggttc gcctcgtcgc
     1861 ggagcgtccg ccacggatcc tcgtaggccg gcagcacgta ttccggcgaa agctcgagcg
     1921 cctgcgcgag ctcggtggcg aggcgccgcg catcttcctc cgtgttcttg ccgccttccg
     1981 ggggcctcgc gtcctcgtct tcgcgctccc acaacgcgac cccgtccttg cgccagaagc
     2041 acgacagcgc ccagcgcggc agcggctcgc cgggatacca cttgccctgg ccgaagtgca
     2101 gcagaccgcc cggcgcgaag cgcttgcgca gcctgtacag gagcgcggtg gcgagctcgt
     2161 gcttcgccgg cgacatcgcg gcgtagttcc actcggggcc ttccatgtcg tcgatggaga
     2221 tgaa
//
LOCUS       DASVII010000220         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_262180,
            whole genome shotgun sequence.
ACCESSION   DASVII010000220 DASVII010000000
VERSION     DASVII010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262180"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="murD"
                     /locus_tag="VF309_02815"
     CDS             complement(<1..966)
                     /gene="murD"
                     /locus_tag="VF309_02815"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7465545.1"
                     /translation="MTNVEWQGKSVLVLGLGDTGLSSVRWLARRGARVRAADTRKAPP
                     ALGALRAQFPTVPVELGAFRTALLEGVDAVVASPGVALREPVLRAALERGIEVVGDVE
                     VFAREVARRSPGARVLAITGTNGKSTVTALAGAMGRAAGYETIVAGNIGLPVLDALDS
                     PQAERAALYVLELSSYQLETTSSLALDAAAMLNLTQDHMDRYDSMDDYARAKERIFRH
                     ASKRVVNRDDPRSAAMGGASAFSFGLGVPANDRQWGLDASCTWMRRGDAGVIALGEMA
                     VQGMHNAANAMAAHALLCAIDAPAAPLAQAMREFKGLPHRVELVAE"
     gene            complement(1021..>1909)
                     /gene="mraY"
                     /locus_tag="VF309_02820"
     CDS             complement(1021..>1909)
                     /gene="mraY"
                     /locus_tag="VF309_02820"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7465546.1"
                     /translation="IKTGTPTMGGALILVSVSVTTLLWADLANRYVWIVLLTTLGFGL
                     VGWVDDWRKVVHRNPKGLPARSKFFWQSVIAVFAVSYLAYSARLPQQTELIVPFFKTI
                     AIPLGTVGFVILGYFVVVGTSNAVNLTDGLDGLAIMPTVMIAGALAVFAYVTGNAVFA
                     KYLGFPLIPGAGELAVICGAMTGAGLAFLWFNAYPAEVFMGDVGALALGAALGMIALV
                     VRQEIVLLIMGGVFVVETLSVIIQVASFKLTGRRVFRMAPLHHHYELKGWKENQVVVR
                     FWIITMMLVLFGLSTLKLR"
BASE COUNT          338 a          664 c          626 g          281 t
ORIGIN      
        1 ctcggcgacg agctcgacgc ggtggggcag gcccttgaac tcgcgcatcg cctgcgcgag
       61 cggcgcggcg ggcgcgtcga tcgcgcagag cagcgcgtgc gccgccatcg cgttcgcggc
      121 gttgtgcatt ccctgcaccg ccatctcgcc gagcgcgatc acgcccgcgt cgccgcgccg
      181 catccacgtg cacgacgcat cgagtcccca ttgcctgtcg ttcgccggca cgccgaggcc
      241 gaacgagaag gcgctcgcgc cgcccatcgc ggcgcttcgc ggatcgtcgc gattgacgac
      301 gcgcttcgac gcgtggcgga agatccgctc cttggcgcgc gcatagtcgt ccatggagtc
      361 gtagcgatcc atgtggtcct gcgtgaggtt gagcatcgcc gcggcgtcga gggcgaggct
      421 cgaggtcgtc tcgagctggt agctcgacag ctcgagcacg tacagcgcgg cgcgctcggc
      481 ctgcggcgaa tcgagcgcat cgagcaccgg caagccgatg ttgcccgcga cgatcgtctc
      541 gtagcccgcg gcgcggccca tggcgccggc gagggccgtc accgtggact tgccgttggt
      601 cccggttatt gccagcacgc gagctcccgg agagcgccgc gcgacttcgc gggcgaagac
      661 ctcgacgtcg ccaaccacct caatgccgcg ctcgagcgcg gcgcgcagga ccggctcgcg
      721 tagcgcaacg cccggactcg caacgaccgc gtccacgcct tccaggagcg ccgtgcgaaa
      781 ggctccgagc tcaacgggaa ccgtggggaa ttgcgcgcgg agcgctccca gggccggagg
      841 cgcctttcgc gtatcggcag cacgcacccg cgcaccgcgc cgcgcgagcc agcgaacgct
      901 cgatagcccc gtatcaccca ggccgagaac gaggacgctt tttccttgcc attccacatt
      961 cgtcacctcg cgcggttctt gttgaaagga ttcaatggac gctctgcatg gaaagtgaat
     1021 tcatcgcagc ttcagcgtgg aaaggccgaa gagcaccagc atcatcgtga tgatccagaa
     1081 gcgaacgacg acctggttct ccttccagcc cttcaattcg tagtggtgat gcaggggcgc
     1141 catgcggaag acgcgcctgc cggtcagctt gaacgaggcg acctggatga tcaccgagag
     1201 cgtctccacc acgaagacgc cgcccatgat cagcagcacg atctcctggc gcacgacgag
     1261 ggcgatcatc ccgagcgccg cgcccagcgc gagcgccccc acgtcgccca tgaagacttc
     1321 cgcgggatag gcgttgaacc acaggaaggc gagccccgcg ccggtcatcg cgccgcagat
     1381 caccgcgagc tcccccgcgc cggggatgag cggaaagccg agatacttcg cgaacacggc
     1441 gttccccgtc acgtacgcga acaccgcgag cgccccggcg atcatcacgg tcggcatgat
     1501 cgcgaggccg tcgaggccgt cggtgaggtt caccgcgttc gaggtcccga ccacgacgaa
     1561 gtagccgagg atcacgaagc cgaccgttcc cagcgggatc gcgatcgtct tgaagaacgg
     1621 cacgatcagc tcggtctgct gcggcagcct cgcgctgtac gcgagatagg acaccgcgaa
     1681 caccgcgatc accgattgcc agaagaattt cgatcgggcc gggagccctt tcggattgcg
     1741 gtgcacgacc ttgcgccagt cgtcgaccca gccgacgagt ccgaagccca gcgtcgtgag
     1801 gagcacgatc cacacgtagc ggttggcgag gtccgcccag aggagcgtcg tgaccgacac
     1861 cgagacgagg atcagcgcgc cgcccatcgt cggcgttccc gtcttgatg
//
LOCUS       DASVII010000221         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_10818, whole
            genome shotgun sequence.
ACCESSION   DASVII010000221 DASVII010000000
VERSION     DASVII010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10818"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2387
                     /gene="putA"
                     /locus_tag="VF309_02825"
     CDS             <1..2387
                     /gene="putA"
                     /locus_tag="VF309_02825"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX7465547.1"
                     /translation="PGISIKLSALHPRYEFAQRERVRAELAPRVLALVERARRGDVGV
                     TLDAEEADRLDLSLDIFEPVFAAPALAGWEGLGLAVQAYQKRAPFMIDWLADLARRER
                     RRIMVRLVKGAYWDSEVKRAQMQGLAGYPVYTRKANTDVSYLACAGKILAAPDAFYGQ
                     FATHNAHTVATILERAAAGQPFEFQRLHGMGEDLYEQVIAQGYACRVYAPVGSHEDLL
                     PYLVRRLLENGANTSFVNRIADAAIPIESVIADPVAIAASQPAANPKIPLPAALYGDR
                     ANSAGFVFADESFAAPLLEQFTALAARTDFAAAPLVSGKDVAGLRREARDPSSGRTIG
                     RVDDATAQIARLALAAASGARIPEAGERAAILERAADALEANRVELLALLAREAGKTL
                     PDGLGEVREAADFCRYYAMLARRHFARPEELAGPTGESNRLQLTGRGTFVCVSPWNFP
                     LAIFVGQVAAAFAAGNAVVAKPAEQTPLVAYRAVKILHGAGVPVDALHLVPGDGGTVG
                     AALVSDPRIAGVAFTGSTETARAINRALAARDGPIVPFIAETGGLNAMFVDSSALAEQ
                     VVSDVIASAFNSAGQRCSALRILCLQEEIAPRVIEILAGAMAELKLGDPARLDTDVGP
                     VIDAEALAMLEAHAARMDREAKLLCKAAMPVDRNAGMFFAPRAYEIANLDAIDREVFG
                     PILHVVRYGSADLGSIVDAVNAKGYGLTMGIHSRIEDTVEFVRRRACAGNLYVNRNMI
                     GATVGVQPFGGEGLSGTGPKAGGPHYLFRFANERTFTVNTAAAGGNASLIAGTE"
BASE COUNT          348 a          872 c          866 g          371 t
ORIGIN      
        1 ggcccggcat ctcgatcaag ctctcggcgc tgcatccgcg ctacgaattc gcgcagcgcg
       61 agcgcgtgcg cgcggagctc gcgccgcgcg tcctcgccct ggtcgagcgc gcgcgccgcg
      121 gcgacgtcgg ggtcacgctc gacgccgagg aggcggaccg cctcgacctg tcgctcgaca
      181 tcttcgagcc cgtgttcgcc gcgccggcgc tcgcgggctg ggagggcctg ggactcgcgg
      241 tccaggcgta ccagaagcgc gcgcccttca tgatcgactg gctcgcggat ctcgcgcggc
      301 gcgagcgccg ccgcatcatg gttcgcctgg tgaagggcgc gtactgggat agcgaggtga
      361 agcgcgcgca gatgcagggg ctcgcgggct atcccgtcta cacgcgcaag gcgaacaccg
      421 acgtttcgta tctcgcctgc gccgggaaga tcctggcggc gcccgacgcg ttctacggcc
      481 agttcgcgac ccacaacgcg catacggtgg cgacgatcct cgagcgcgcc gccgccgggc
      541 agccgttcga gttccagcgc ctgcacggca tgggcgagga tctctacgag caggtgatcg
      601 cgcagggata cgcgtgccgc gtctacgcgc ccgtgggaag ccatgaagac ctccttccgt
      661 atctcgtgag gcgattgctc gagaacggcg cgaacacttc gttcgtgaac cgcatcgccg
      721 atgcagcgat tcccatcgag agcgtgatcg ccgatccggt ggcgatcgcg gcttcccagc
      781 cggcggcgaa tccgaagatc ccgctgcccg cggcgctcta cggcgatcgc gccaattcgg
      841 cgggcttcgt tttcgccgat gaatcgttcg cggcgccgct cctcgagcaa ttcaccgcgc
      901 tagccgcgcg caccgacttc gccgccgcgc ccctcgtgtc cggcaaggac gtcgcgggcc
      961 tgcggcgaga ggcgcgcgat ccctcgagcg gccggacgat cgggcgggtc gacgacgcaa
     1021 ccgcgcagat cgcgcggctc gcgctcgccg ccgcgagcgg cgcgcgcatt cccgaggcgg
     1081 gcgagcgcgc cgcgatcctc gagcgcgcgg ccgatgcgct cgaggccaat cgcgtcgagc
     1141 tcctcgcgct cctcgcgcgc gaggcgggca agacgcttcc cgacggccta ggcgaggttc
     1201 gcgaggccgc cgacttctgc cgctactacg ccatgctcgc gcggcgtcat ttcgcccggc
     1261 ccgaggagct cgccggcccc acgggcgagt ccaatcgcct gcagctcacc gggcgcggga
     1321 ctttcgtttg cgtgagcccg tggaattttc ccctggccat cttcgtgggg caggtggccg
     1381 cggcgttcgc cgcgggcaac gcggtggtcg cgaagccggc ggagcagacg ccgctcgtcg
     1441 cgtatcgcgc ggtgaagatc ctccatgggg cgggcgttcc cgtggatgcg ctccatctcg
     1501 tgccgggcga cggcgggacg gtcggcgcgg cgctggtgag cgatccacgc atcgcgggcg
     1561 ttgccttcac cggctcgacc gagaccgcgc gggcgatcaa ccgcgcgctc gccgcgcgcg
     1621 acgggcccat cgttcccttc atcgccgaga ccggcgggct gaacgcgatg ttcgtcgact
     1681 cgagcgcgct tgccgagcag gtcgtctccg acgtgatcgc ctcggccttc aatagcgccg
     1741 gccagcgctg ctcggcgctg cgcatcctgt gcctgcagga ggagatcgcg ccccgggtga
     1801 tcgagatcct cgccggcgcc atggcggagc tgaagctcgg cgatcccgca cgcctcgaca
     1861 ccgacgtggg gcccgtgatc gacgccgagg cgctcgcgat gctcgaggcg cacgcggcga
     1921 ggatggatcg cgaggcgaag ctgctctgca aggcggcgat gcccgtcgac cggaacgcgg
     1981 gaatgttctt cgcgccgcgc gcgtacgaga tcgcgaacct cgacgcgatc gatcgcgagg
     2041 tgttcgggcc gatcctgcac gtggtgcgct acgggtcggc cgatctcggc tcgatcgtcg
     2101 acgccgtgaa cgccaagggc tacgggctca ccatgggcat ccacagccgc atcgaggaca
     2161 ccgtcgaatt cgtgcgccgc cgcgcgtgcg cgggcaatct ctacgtgaat cgcaacatga
     2221 tcggcgcgac cgtgggcgtg cagcccttcg gcggcgaggg cctgtcggga accggtccca
     2281 aggccggcgg gccgcactat cttttccggt tcgcgaacga gcgcaccttc acggtgaaca
     2341 ccgccgccgc gggcggcaac gcctcgctca tcgccggcac cgaataagag tgatcttcga
     2401 caggaagctc gtcgaagggc gcctgatccg gcgctacaag cgattcctcg cggacgt
//
LOCUS       DASVII010000222         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_262240,
            whole genome shotgun sequence.
ACCESSION   DASVII010000222 DASVII010000000
VERSION     DASVII010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262240"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..689
                     /gene="gmk"
                     /locus_tag="VF309_02830"
     CDS             57..689
                     /gene="gmk"
                     /locus_tag="VF309_02830"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7465548.1"
                     /translation="MESPVDAQMRGNLFVVVAPSGAGKTSLVSALLEQEPNIKLSISY
                     TTRAPRSGEEHARDYHFVPREDFEKMIAAGDFLEHASVYGNYYGTSRRWIDNELNGDH
                     DVLLEIDWQGAKQVRKLFAHMVGIFILPPSLAELRKRLESRGKDSPETIARRTASTRE
                     DISHVLEFEYIIVNDRFEAALTDLRSIVRAARVSRTQQSVRLASLLDEFK"
     gene            704..925
                     /gene="rpoZ"
                     /locus_tag="VF309_02835"
     CDS             704..925
                     /gene="rpoZ"
                     /locus_tag="VF309_02835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7465549.1"
                     /translation="MARITVDDCMKEFTNRFELTLAATNRARQIALGATPLVEPNKDK
                     PTVIALREIASGKLGRELLNPPAPTPPVL"
     gene            967..>2324
                     /locus_tag="VF309_02840"
     CDS             967..>2324
                     /locus_tag="VF309_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX7465550.1"
                     /translation="MPALGVQSVASGAKVTGTSLPGPGPDELTAVLGYLKPKDVQHIE
                     EAYEVARAAHAGQYRQSGEPYITHPLAVAKILAEWHLDPQALMAALLHDVVEDTPTTK
                     TEIAKRFGKAVAELVDGVSKIDRIEFATLQHAQAENFRKMLLAMARDVRVILIKLADR
                     LHNMRTLEAVPPEKQERIARETLDIYAPIANRLGLNALYYELEDLGFHFLHPNRFRVL
                     DKALKKARGNRRDAVGKIQEAIQARLKEFKVEAQVTGREKHISSIYKKMQEKNISFSE
                     VFDIYGFRIIVPDVPSCYLALGALHTLYKPIPGKFKDYIAISKANGYQSLHTTLFGPF
                     GMPIEIQIRTPEMHKVSEAGVASHWMYKSAEDQKDVQLKTHQWLQSLLEIQSESGDAL
                     EFLEHIKVDLFPDEVYVFSPKGKIFALPKGATPIDFAYSVHTDVGNHCIAAKVDGEPV
                     PLG"
BASE COUNT          462 a          792 c          715 g          355 t
ORIGIN      
        1 cgaatagatg agcgcgctcc ggaaaatcgg caacgggaac gccgttggac cggacgatgg
       61 aatcgccggt cgacgcccag atgcgcggta accttttcgt cgtcgtcgcg ccgtcgggcg
      121 cgggcaagac gagcctcgtc agcgcgctcc tcgagcagga gcccaacatc aagctgtcga
      181 tctcgtacac cacgcgcgcg ccgcgctccg gcgaggagca cgcgcgcgac taccacttcg
      241 tgccgcgcga ggatttcgag aagatgatcg cggcgggcga ttttctcgag cacgccagcg
      301 tctacggcaa ctactacggc accagccggc gctggatcga caacgagctg aacggggacc
      361 acgacgtcct cctcgagatc gattggcagg gcgcgaagca ggtgcgcaag ctctttgcgc
      421 acatggtcgg gatcttcatc ctgcctccct ccctcgccga gctgcgcaag cgcttggaat
      481 cacggggaaa agactctccc gagacgatcg ccaggcgcac cgcgagcacc cgcgaagata
      541 tctcgcatgt tcttgaattt gaatatataa ttgtcaatga ccgcttcgag gcggcattga
      601 cggacctgag atcgatcgtg cgagcggcca gagtgtcgcg cacgcagcag tccgttcgtc
      661 tcgcttcgct gctcgacgaa ttcaagtaac tggagaccgc gccatggccc gcatcaccgt
      721 tgacgactgc atgaaggaat ttaccaaccg cttcgagctc acgctcgccg cgaccaatcg
      781 cgcgcgccag atcgccctcg gcgcgacgcc gctggtcgag cccaacaagg acaagcccac
      841 cgtgatcgcg ctgcgtgaga tcgcgagcgg caagctcggc cgcgagctgc tcaacccccc
      901 ggcgcccacg ccgccggtgc tctgacgatc cggtagcacg acgggttgtc cgagccgaac
      961 gcgatcatgc ctgcgctcgg tgtccaatcc gtcgcaagcg gtgccaaggt cacggggact
     1021 tcgctccccg gcccgggccc cgacgagctg acggcggtcc tcggttacct caagccgaag
     1081 gacgtccagc acatcgagga agcgtacgag gtcgcccggg cggcccacgc ggggcagtat
     1141 cgccagagcg gcgagccgta catcacccac ccgctcgcgg tggcgaagat cctcgccgaa
     1201 tggcacctcg acccgcaggc gctgatggcc gcgctcctgc acgacgtggt cgaggacacg
     1261 ccgaccacca agacggagat cgccaagcgc ttcggcaagg cggtggcgga gctggtcgac
     1321 ggcgtctcga agatcgatcg catcgagttc gcgacgctcc agcacgcgca ggccgagaac
     1381 ttcaggaaga tgctgctcgc gatggcccgc gacgtgcgcg tgatcctcat caagctcgcc
     1441 gatcgcctgc acaacatgcg cacgctcgaa gcggtgcctc cggagaagca ggagcgcatc
     1501 gcccgcgaga cgctcgacat ctacgccccg atcgcgaacc ggctcgggct gaacgcgctc
     1561 tactacgagc tcgaggacct cggcttccac ttcctgcatc ccaaccgctt ccgcgtcctc
     1621 gacaaggcgc tgaagaaggc gcgcggcaac cgccgcgacg cggtcggcaa gatccaggag
     1681 gcgatccagg cgcgcctgaa ggaattcaag gtcgaggcgc aggtcaccgg ccgcgagaag
     1741 cacatctcct cgatctacaa gaagatgcag gagaagaaca tctcgttctc ggaggtgttc
     1801 gacatctacg gcttccgcat catcgttccc gacgtgccgt cgtgctacct cgcgctgggc
     1861 gcgctgcaca cgctctacaa gccgatcccc ggcaagttca aggactacat cgccatctcg
     1921 aaggcgaacg gctaccagtc cctgcacacc acgctcttcg gcccgttcgg catgccgatc
     1981 gagatccaga tccgcacccc cgagatgcac aaggtctcgg aggccggggt ggcgtcccac
     2041 tggatgtaca agagcgccga ggaccagaag gacgtgcagc tgaagacgca tcaatggctg
     2101 cagtcgctcc tcgagatcca gagcgaatcg ggcgacgcgc tcgaattcct cgagcacatc
     2161 aaggtcgacc tcttccccga cgaggtatac gtcttctcgc cgaagggcaa gatattcgcg
     2221 ctgccgaagg gcgccacgcc gatcgacttc gcctactcgg tgcacaccga cgtcggcaac
     2281 cactgcatcg cggcgaaggt cgacggggag ccggtcccgc tggg
//
LOCUS       DASVII010000223         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_370445,
            whole genome shotgun sequence.
ACCESSION   DASVII010000223 DASVII010000000
VERSION     DASVII010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370445"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF309_02845"
     CDS             complement(<1..245)
                     /locus_tag="VF309_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX7465551.1"
                     /translation="MCSRYFLDADGNVIAYTFRVPAQPVRKRFNIAPTQAAPVIRVRE
                     GAREMAMLRWGLVPYWAKDLAVGTKMINARSEGVEAKP"
     gene            311..841
                     /locus_tag="VF309_02850"
     CDS             311..841
                     /locus_tag="VF309_02850"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7465552.1"
                     /translation="MKIIQWTLAVLGALVLLLVAGGFFIPSTFKVERSTVINAAPRKV
                     YDLVVEPKQWTKWSVWNQRDPNMKITYKGPPFGMGAKWEWVSKTEGNGSMEFTRVEPD
                     RVVEYALFFPDYNMRSAGAITLEPAGASTRVTWSNAGDVGGNPVKHYLTLTMDRMVGP
                     DFAAGLANLKALAEQP"
     gene            838..1587
                     /locus_tag="VF309_02855"
     CDS             838..1587
                     /locus_tag="VF309_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX7465553.1"
                     /translation="MTDSLARAPATGRACPSCGAAMRQESYERRLEGRVDLDFCDACQ
                     AIWFDAYESTQLTPGAVMKVFEAIARRREVQPRALADVCRCPACRRALALTHDIQRAN
                     RITYYRCPEGHGRFTTYYQFLREKNFVRSLTVPEIEKLKAVVKQVRCTSCGAPVSLER
                     DPQCPFCGAPISMLDPDAATRALAELASEEQKRRAVDPHAAIDALLAGQRSARATGAW
                     RPYDFQPVSAGWTSGAVDLIGEAVDFFMNLD"
BASE COUNT          273 a          543 c          553 g          247 t
ORIGIN      
        1 ggcttcgcct ccacgccctc ggagcgcgcg ttgatcatct tggtgccgac ggccaggtcc
       61 ttcgcccagt agggcaccag gccccagcgc aacatcgcca tctcgcgcgc gccctcgcgc
      121 acgcggatca cgggcgcggc ctgcgtgggc gcgatgttga agcgcttgcg caccggctgc
      181 gccggcacgc ggaacgtgta ggcgatgacg ttgccgtcgg cgtcgaggaa atagcggctg
      241 cacatgggaa gaaattggga gccggtcgcg gtccggtccc cttatactaa ccgcgggagg
      301 gtcgaaaaaa atgaaaatca tccaatggac gctggcggtg ctcggggcgc tggtgctgct
      361 gctggtggcg ggcggcttct tcatcccgtc gaccttcaag gtggagagga gcaccgtcat
      421 caatgccgcg ccgcgcaagg tctacgacct ggtcgtcgag ccgaagcaat ggacgaagtg
      481 gagcgtgtgg aaccaacgcg accccaacat gaagatcacc tacaagggcc cgcctttcgg
      541 catgggcgcg aagtgggagt gggtgagcaa gacggaaggc aacggcagca tggagttcac
      601 ccgcgtcgag cccgatcgcg tcgtcgagta cgcgctcttc ttccccgact acaacatgcg
      661 ctcggcgggc gcgatcaccc tcgagcccgc gggcgcctcc acgcgggtca cgtggagcaa
      721 cgcgggcgat gtcggcggca atccggtcaa gcactacctc acgctcacca tggatcgcat
      781 ggtcgggccg gatttcgccg cggggctcgc caacctgaag gcgctcgccg agcagccgtg
      841 accgacagcc tcgcgcgcgc gcccgcgacg gggcgagcct gtcccagctg cggcgcggcg
      901 atgcgccagg agagctatga gcgccgcctc gagggacgcg tcgacctcga tttctgcgat
      961 gcgtgccagg ccatctggtt cgacgcctac gaaagcactc agctcacgcc cggcgcggtg
     1021 atgaaggtct tcgaggcgat cgcccggcgc cgcgaggtcc agccgcgcgc gctggccgac
     1081 gtgtgccgct gtcccgcctg ccggcgcgcg ctcgccctca cccacgacat ccagcgcgcc
     1141 aaccgcatca cctactatcg ctgtcccgaa ggccacgggc ggttcacgac ctactaccag
     1201 ttcctgcgcg agaagaactt cgtgcgctct ctcacggtgc ccgagatcga gaagctgaag
     1261 gcggtggtga agcaggtgcg ctgcacgagt tgcggagcgc cggtgagcct ggagcgcgac
     1321 ccccaatgcc cgttctgcgg ggcgccgatc tcgatgctcg accccgatgc ggcgacccgc
     1381 gccctcgccg agctggcgag cgaggagcag aagcgccgcg cggtcgatcc tcatgcagcg
     1441 atcgacgcgc tcctcgccgg gcagcgctcg gcgcgcgcga ccggcgcgtg gcgcccgtat
     1501 gatttccagc cggtctccgc ggggtggacg agcggcgcgg tagaccttat cggagaagcg
     1561 gtcgattttt tcatgaacct cgattgacgg gagacggacg atgaatcgca tcctgg
//
LOCUS       DASVII010000224         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_10913, whole
            genome shotgun sequence.
ACCESSION   DASVII010000224 DASVII010000000
VERSION     DASVII010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10913"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VF309_02860"
     CDS             <1..642
                     /locus_tag="VF309_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEX7465554.1"
                     /translation="RGTALRHIEFFGLSEKRVLLIIVASDGEVQNRVLVTDRSYTPSQ
                     LVEAGNYINQNYAGVEFDQLRQKVRSELMDLHRDISGLMQAAIQTGSDAMRPIAGDGV
                     VISGGRNLLEVNELSGDMQRLRRLFDLFESKTQLLDILETSERAEGVKIFIGGESHLV
                     PLDECSIVTAPYEVDGVVVGTVGVIGPTRMAYERVIPIVDVTARLVSSALSQH"
     gene            643..1623
                     /gene="hemH"
                     /locus_tag="VF309_02865"
     CDS             643..1623
                     /gene="hemH"
                     /locus_tag="VF309_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7465555.1"
                     /translation="MSEARATGVLLVNLGTPDAPTPAAVRRYLREFLWDPRVVQIPRP
                     LWWLILNFAILPFRPAKAARKYATIWREDGSPLRVYLGRQVQLLRGYLGEQLGRPVPV
                     AGAMRYGKPSIATGLGELAARERVRIVVLPLYPQYAGSTTGSSKDAVARALNKMRPAP
                     TLKWVEDFHAHPAYVKAIAKNVNEYWMKHGRPDRLVMSFHGLPRAVVAAGDPYERQCR
                     ESARLIATELGWNDARTIVSFQSRFGAQEWLQPYTAQTLAALGRDGTARVDVICPGFA
                     ADCLETLEEIAQEGRDTFRNAGGKEFHYIPTTNDTPSWMTAIATIVTENL"
     gene            1623..1973
                     /locus_tag="VF309_02870"
     CDS             1623..1973
                     /locus_tag="VF309_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7465556.1"
                     /translation="MLMLLARWIVNAAALVLVAYIYPGVHVDSFMAALFAALVLGLVN
                     AVIRPLLVILTLPVTLLTLGLFLFVINALCFWLVAEMVRGFTVGGFGPALVGSILYSL
                     VTLVTSWLLFSRRK"
     gene            2145..2393
                     /gene="grpE"
                     /locus_tag="VF309_02875"
     CDS             2145..2393
                     /gene="grpE"
                     /locus_tag="VF309_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7465557.1"
                     /translation="MESLREGVELTARQLDSAFDKFAIRAISPAGERFDPHRHQAIGQ
                     VESDAEPNTVANVLQKGYLLNDRVLRPALVMVTKPRSR"
     gene            2476..>3721
                     /gene="dnaK"
                     /locus_tag="VF309_02880"
     CDS             2476..>3721
                     /gene="dnaK"
                     /locus_tag="VF309_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7465558.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGGKPKVIENSEGARTTPSVVAYSD
                     SGEILVGAPAKRQAVTNAKNTIYAVKRLIGRTFEEKEVQKDIGLMPYRITKADNSDAW
                     VEVRGEKIAPQQVSAEILRKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVRRIINEPTAGALAFGLDKKEGDRKIAVYDLGGGTFDISIIEIAAVEGEHQFEV
                     LSTNGDTFLGGEDFDQRLMDYVADEFKREQGVDLRKDILALQRLKEAAEKAKIELSAT
                     QQTEVNLPYITMDQSGPKHLVMKITRAKFESLVEELIEKTVAPCLTAIKDSGVKLSDI
                     SDVILVGGQTRMPKVQDKVKEFFGKEPRKDVNPDEAVAVGAAIQGGVMQGDVKDVLLL
                     DVTPLSLGIETLGGVMTKLIKKN"
BASE COUNT          688 a         1247 c         1205 g          581 t
ORIGIN      
        1 cgcggaacgg cgctgcgcca catcgagttc ttcgggcttt ctgagaagcg cgtgctgctc
       61 atcatcgtcg ccagcgacgg cgaggtgcag aaccgcgtac tcgtcaccga tcgcagctac
      121 acgccttcgc agctcgtcga ggccgggaac tacatcaacc agaactacgc cggggtggag
      181 ttcgaccagc tgcgccagaa ggtgcgcagc gagctgatgg acctgcaccg cgacatctcg
      241 ggcctcatgc aggccgcgat ccagacggga agcgacgcga tgcgcccgat cgccggcgac
      301 ggcgtcgtca tctcgggcgg caggaacctc ctcgaggtga acgagctgtc cggcgacatg
      361 cagcgcctgc gtcgcctctt cgacctcttc gagtcgaaga cgcagctcct cgacatcctc
      421 gagacgagcg agcgcgccga gggcgtgaag atcttcatcg gcggcgagtc ccatctggtg
      481 ccgcttgacg agtgctcgat cgtgaccgcg ccctacgagg tcgacggcgt ggtggtgggc
      541 accgtgggcg tgatcggtcc cacgcgcatg gcctacgagc gcgtgatccc tatcgtggac
      601 gtgaccgccc ggctcgtttc ctccgcgctt tcgcagcact gaatgagcga ggctcgcgcg
      661 acgggcgtgc tgctcgtgaa ccttggcact cccgacgcgc ccacgcccgc ggccgtgcgc
      721 cggtacctgc gcgaattcct gtgggacccg cgcgtggtgc agatcccgcg cccgctgtgg
      781 tggctcatcc tcaacttcgc gatcctgccg tttcgcccgg cgaaggcggc ccggaaatac
      841 gcgaccatct ggcgggagga cggctccccg ctgcgggtct acctcgggcg gcaggtgcag
      901 ctcctgcgcg gctacctcgg cgagcaactc gggaggccgg tgcccgtcgc cggggcgatg
      961 cgctacggaa agccttcgat cgccacgggc ctgggggaac tcgcggcgcg cgagcgtgtc
     1021 cggatcgtcg tgctgccgct ctatccgcag tacgcgggca gcacgaccgg cagcagcaag
     1081 gacgcggtgg cgcgcgcgct gaacaagatg cgcccggcac cgacgttgaa gtgggtcgag
     1141 gatttccacg cgcaccccgc atacgtgaag gcgatcgcga agaacgtgaa cgaatactgg
     1201 atgaagcacg ggcgccccga ccgcctggtg atgagcttcc acggcttgcc ccgcgcggta
     1261 gtcgcggccg gcgacccgta cgagcggcaa tgccgcgaga gcgcgcgcct catcgcgacg
     1321 gagctcggat ggaacgacgc ccgcacgatc gtgagcttcc agtcgcgctt cggcgcccag
     1381 gagtggctgc agccctacac cgcgcagacc ctcgcggcgc tggggcggga cggcacggcg
     1441 cgcgtggacg tgatctgccc cggcttcgcg gccgattgcc tcgagacgct cgaggagatc
     1501 gcgcaggaag gccgggacac tttccgaaat gcgggcggca aggagttcca ttacatccct
     1561 accacgaacg acaccccttc ctggatgacc gcgatcgcaa cgatcgtcac ggagaacctc
     1621 tgatgctgat gctcctcgca cgctggatcg tgaacgccgc cgcgctggtg ctggtggcct
     1681 acatctaccc cggagtccat gtggacagct tcatggccgc gctcttcgcg gccctggtcc
     1741 tcgggctggt gaacgcggtg atccggccgc tcctcgtgat cctcacgctt cccgtcactc
     1801 tcctgaccct gggcctcttc ctcttcgtca tcaacgcgct gtgcttctgg ctggtcgccg
     1861 agatggtccg cggcttcacc gtcggcggat tcggcccggc cctggtgggg tcgatcctct
     1921 acagcctcgt cacgctcgtc acgagctggc tcctgttttc ccggcgaaag taggcgcgcc
     1981 cggccaaaaa aacgccccga tcggggcgat agtgggcgca accgcttgaa atgccccgcg
     2041 acgatcccca tttttggtca agcagttgtt tcccaacgag gattttcccg tgacagacgc
     2101 tgatttcgca ggctcccccg gctccgcgcc ggcccagccc gtcgatcgaa tcgctgaggg
     2161 aaggcgtcga gcttacggcc cgccagctcg actccgcgtt cgacaagttc gcgatccggg
     2221 ccatctcgcc ggcgggcgaa agattcgatc cgcaccgtca ccaggcgatc ggccaggtcg
     2281 agagcgacgc agagcccaat accgtggcca acgtcctcca gaagggctac ctgctaaacg
     2341 accgagtgct gcgcccggcg ctcgtgatgg tcaccaagcc gcgctcgcga tgatttcgcc
     2401 cagccccttg aagccgcgcc ccatgcaccc aattttccaa tcaggtcaaa aacaccctca
     2461 ctgaagggaa cgaacatggc aaaaatcatc ggaatcgacc tcggcaccac caactcctgc
     2521 gtcgcagtga tggagggcgg caagcccaag gtcatcgaga acagcgaagg cgcgcgcacc
     2581 acgccttccg tggtcgcgta ctcggatagc ggcgagatcc tcgtcggcgc gcccgccaag
     2641 cggcaggccg tcaccaacgc gaagaacacc atctacgcgg tgaagcgcct catcgggcgc
     2701 acgttcgagg agaaagaagt ccagaaggac atcggcctca tgccgtacag gatcaccaag
     2761 gcggacaact ccgacgcctg ggtcgaggtg cgcggcgaga agatcgcgcc gcagcaggtt
     2821 tccgccgaga tccttcgcaa gatgaagaag accgccgagg actacctcgg cgagccggtg
     2881 accgaagcgg tcatcacggt tcccgcctac ttcaacgatt cgcagcgcca ggcgaccaag
     2941 gacgcgggac gcatcgcggg cctcgaggtg aggcgcatca tcaacgagcc caccgccgga
     3001 gcgctggcgt tcggcctcga caagaaggaa ggcgaccgca agatcgcggt ttacgacctg
     3061 ggcggcggca ccttcgacat ctcgatcatc gagatcgcgg cagtcgaggg cgagcaccag
     3121 ttcgaggtgc tctccaccaa cggtgacact ttcctgggcg gcgaggactt cgaccagcgc
     3181 ctgatggact acgtcgcgga cgagttcaag cgcgagcagg gcgtcgatct gcgcaaggac
     3241 atcctggcgc tgcagcgcct gaaagaggca gccgagaagg ccaagatcga gctgtccgcc
     3301 acccagcaga ccgaggtcaa cctgccctat atcaccatgg accagtcggg gccgaagcac
     3361 ctggtcatga agatcacccg ggccaagttc gagagcctgg tggaggagct gatcgagaag
     3421 accgtcgcgc cctgcctcac cgcgatcaag gattcgggcg tgaagctctc cgacatcagc
     3481 gacgtgatcc tggtcggcgg ccagacgcgc atgccgaagg tgcaggacaa ggtgaaggag
     3541 ttcttcggca aggagccgcg caaggacgtg aaccccgacg aggcggtggc ggtcggcgcg
     3601 gcaatccagg gcggagtgat gcaaggcgac gtgaaggatg tcctcctcct cgacgtcacg
     3661 ccgctctcgc tgggcatcga gacccttggc ggcgtcatga cgaagctcat caagaagaac
     3721 a
//
LOCUS       DASVII010000225         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_154210,
            whole genome shotgun sequence.
ACCESSION   DASVII010000225 DASVII010000000
VERSION     DASVII010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154210"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF309_02885"
     CDS             complement(<1..331)
                     /locus_tag="VF309_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465559.1"
                     /translation="MLREIPETRQIRGEPRRRWFNSEWMDLYVWYDTAGAPLGFQLCY
                     GKPLDEKALTWFRPDDFSHMAVDHGGPAGRGKETPLLVLDGLFLPGPVSTEFVREAGE
                     LPEDVAQL"
     gene            complement(324..1487)
                     /gene="solA"
                     /locus_tag="VF309_02890"
     CDS             complement(324..1487)
                     /gene="solA"
                     /locus_tag="VF309_02890"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX7465560.1"
                     /translation="MYDLIIVGLGAVGAASAYQAAKRGAKVLGIDRFAPPHALGSSHG
                     DTRITRIAIGEGEHYTPIVRRSHQIWRDIEAKTGEELLVVTGGLIISSSARRATTHVA
                     NFFENTLAAARRFGVEHELLDAGNLRELFPQFNVSDNEVGYYEPGAGYLRPEACIRAE
                     LALAEENGAELRRNERVDDIADEGGAVRVRTEAAEYRARQVIVAAGAWLPQLLDADLA
                     RHFRVTRQVLYWFETAAPIADFTAPHFPIWIWELQDREHVIYGFPAIGGMEAGIKIAT
                     EQYARQAHPDESSRAVTEDETREMRRNLVAPYLPGVGARCIKAVSCLYTATPDFHFAI
                     GRHPRMPNVIVASACSGHGFKHSAALGEALAQLSCEARSTIDLAPFSLERFHA"
     gene            complement(1488..>2152)
                     /locus_tag="VF309_02895"
     CDS             complement(1488..>2152)
                     /locus_tag="VF309_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7465561.1"
                     /translation="YAKLPYDPIKDFEPISNLVLTPQVIVVGSQTPYKTLKDLLDAAR
                     AKPGEIAYGSLGTGSTSHLTMEALQQAAGVKMNHIPFKGGAEAQTQIMGGVLPAMSDT
                     ITGVLAQVRAGKLRALGVAILKRSPYLPDVPTIAEQGFPGFESVGWIGLCAPAKTPAA
                     ILDKLNAEVLKMLGSPDVKAKIEDLAFTPVGDSRAQFAAWIRSEIAKWSKIAKDSGAR
                     AD"
BASE COUNT          322 a          725 c          753 g          352 t
ORIGIN      
        1 cgagctgggc cacgtcctcg gggagctcgc cggcttcccg gacgaattcc gtcgacaccg
       61 ggccggggag gaagaggccg tcgaggacga ggagcggcgt ttccttgccg cggcccgccg
      121 gcccgccgtg atcgaccgcc atgtggctga agtcgtcggg gcgaaaccac gtgagggcct
      181 tctcgtcgag gggcttgccg tagcagagct ggaagccgag cggcgcgccg gcggtgtcgt
      241 accagacgta gaggtccatc cactccgaat tgaaccagcg ccggcgaggc tcgccgcgaa
      301 tctggcgcgt ctcgggaatc tccctaagca tggaagcgct ccagcgagaa tggagcgagg
      361 tcgatggtgc tgcgcgcctc gcaggagagc tgcgcgagcg cttcgccgag cgcggccgaa
      421 tgcttgaacc cgtgccccga gcatgcggac gcgacgatga cgttcggcat gcgcggatgg
      481 cgcccgatcg cgaagtggaa gtccggcgtc gcggtataga ggcaggagac cgccttgatg
      541 cagcgcgcgc ccactcccgg aagatagggg gcgacgaggt ttcgccgcat ctcgcgagtc
      601 tcgtcctcgg tcacggcgcg gctcgactcg tcgggatgcg cctgcctcgc gtactgctcg
      661 gtggcgatct tgatgcccgc ctccatgccg ccgatcgccg gaaatccgta gatcacgtgc
      721 tcgcggtcct gcagctccca gatccagatc gggaaatgcg gcgccgtgaa atcggcgatg
      781 ggcgccgccg tctcgaacca gtagagcacc tggcgcgtca cgcggaagtg ccgcgcgaga
      841 tccgcgtcga gtagctgcgg aagccatgcg ccggcggcga cgatgacctg gcgcgcgcga
      901 tactcggcgg cctcggtgcg cacgcgcacg gcgccgccct cgtcggcgat atcgtcgacg
      961 cgctcgttgc ggcgcagctc cgcgccgttc tcctcggcga gcgcgagctc ggcgcggatg
     1021 cacgcctcgg gacgcaggta gcccgcgccg ggctcgtagt agccgacctc gttgtcgctc
     1081 acgttgaatt gcgggaaaag ctcgcgcagg ttgccggcat cgagcagctc gtgctcgacg
     1141 ccgaagcggc gcgccgcggc gagcgtgttc tcgaagaagt tcgcgacgtg cgtcgtcgcc
     1201 cgccgcgcgc tgctcgagat gatgaggccg ccggtgacga cgaggagctc ctcgcccgtc
     1261 ttcgcctcga tgtcgcgcca gatctggtgc gagcggcgca cgatcggcgt gtagtgctcg
     1321 ccctcgccga tcgcgatgcg cgtgatgcgc gtgtcgccgt gggacgaccc gagcgcgtga
     1381 ggcggcgcga agcggtcgat gccgagcacc ttcgcgccgc gcttcgcggc ctggtacgcg
     1441 ctcgccgcgc ccaccgcccc caatccgacg atgatgaggt cgtacaacta gtcggcccgg
     1501 gcgccgctat ccttggcgat cttcgaccac ttcgcgattt ccgacctgat ccacgcggcg
     1561 aactgcgcgc gcgaatcccc gaccggagtg aacgcgaggt cctcgatctt tgccttcacg
     1621 tcgggagagc cgagcatctt cagcacctcg gcgttcagct tgtcgaggat cgcggccggc
     1681 gtcttcgcgg gggcgcagag cccgatccag ccgacgctct cgaagcccgg gaaaccctgc
     1741 tccgcgatgg tgggcacgtc cggcaggtag ggcgagcgct tcaggatcgc gacacccagg
     1801 gcgcggagct tgcccgccct cacctgcgct agcacgccgg tgattgtgtc agacatcgcg
     1861 ggaagcaccc cgcccatgat ctgcgtctgc gcctcggcgc cgcccttgaa ggggatgtga
     1921 ttcattttca cgcccgccgc ctgctgcaac gcttccatgg tgaggtggct cgtcgatccc
     1981 gtgccgagcg acccgtaagc gatttcgccg ggcttcgccc tcgccgcgtc gagcaggtcc
     2041 ttcagggtct tgtacggcgt ctgcgagccg accacgatga cctgcggcgt gagcacgagg
     2101 ttgctgatcg gctcgaagtc cttgatggga tcgtagggaa gcttcgcgta ga
//
LOCUS       DASVII010000226         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_513923,
            whole genome shotgun sequence.
ACCESSION   DASVII010000226 DASVII010000000
VERSION     DASVII010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513923"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VF309_02900"
     CDS             <1..603
                     /locus_tag="VF309_02900"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX7465562.1"
                     /translation="TADNPRGAQYKAHDIRHIVALRGDMPSGIATGGELRYASDLVRF
                     IREETGDWFQIEVACYPECHPQARKPQHDLAAFKAKIDAGANSAITQYFYNADAYFHF
                     VDAAEAMGIDVPIVPGVMPINNFSQLARFSDMCGAEIPRWIRTKLEGFGDDTASIKAF
                     GLDVVTELCDRLLQGGAPGLHFYTMNMAGPSSTIWQRLGL"
     gene            661..1893
                     /locus_tag="VF309_02905"
     CDS             661..1893
                     /locus_tag="VF309_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX7465563.1"
                     /translation="MKTAASPPSEAVPQSSMLASRIRSLPTDEAAALIERAVPGESIE
                     ALLQLNPAMVQAILRELESGPRRFITASAPPEVAAQWKRNASYPSESIGQLMEPSLSV
                     FSPDMTVGEAIEALRPLVRSAFVTYVYVVDSEGKLGGLVTMRDLLFNDRGARLADVML
                     ANPFSLAPELTVAEAMKLTANRHYPQYPVTDADGRLVGLVRGAALFELEAFEITAQAG
                     AMVGVEKEERVATSIAQSFKFRNPWLLINLLTAFAAGGVVALFQGTLDKLVILATFLP
                     VLAGQAGNTGCQALAVTVRGITLGDIKPGGGGALTSKEALLGFANGAVTGVLCGIAMY
                     LYALAQSSPHAILLGVVVTLAMVGSCCISGISGALVPLALKRFGADPATASSIIVTTA
                     TDVTSMGLLLGLAAVMIR"
     gene            1914..2441
                     /locus_tag="VF309_02910"
     CDS             1914..2441
                     /locus_tag="VF309_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465564.1"
                     /translation="MGLRTAFAIILVALASAAGAQEQAFTNRSTELKDRGASDARTLA
                     TLPENTSVKVLARGGGWTRVEAAGQSGWLNVFHLRFAAAVDSSGSSGGAFGGLTSFLG
                     GGRNTSQKANIATTGIRGLSPEDIKNASPDPAALAKAQSYRADKPAAERFAREAKLAA
                     VSIDYVGDSAKGGRR"
     gene            2438..>3034
                     /locus_tag="VF309_02915"
     CDS             2438..>3034
                     /locus_tag="VF309_02915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7465565.1"
                     /translation="MRFALLTAIALACTCANAQFGSLLDLGKKALDKKQQLETANKEF
                     TTDEEIALGEGIASAFLGAAPLHHDQNLQRYVNRVGRWVAMHGERPDLPWTFGVIDTE
                     TINAFAFPGGTVIVSSGLLKRLNSESELAGALAHEVAHVVKRHQIQAIQSQASTDFWA
                     GLGQDVVSDRVARSGGGAVTHMVNQKLVDMGANLVKSGF"
BASE COUNT          488 a         1059 c         1013 g          474 t
ORIGIN      
        1 accgccgaca acccccgtgg cgcccagtac aaggcgcacg acatccggca catcgtggcg
       61 ctgcgcggcg acatgccttc gggcatcgcg actggcgggg agctgcgcta cgcgtcggac
      121 ctggtgcgct tcatccgcga ggagaccggc gactggttcc agatcgaggt cgcgtgctat
      181 cccgagtgcc acccgcaggc gaggaagccg cagcacgatc tcgccgcgtt caaggcgaag
      241 atcgacgcgg gcgcgaactc cgcgatcacg cagtatttct acaatgccga cgcgtacttc
      301 cacttcgtcg acgcggccga ggcgatgggc atcgacgtcc ccatcgtgcc cggcgtgatg
      361 ccgatcaaca acttctcgca gctcgcgcgc ttctccgaca tgtgcggcgc cgagatcccg
      421 cgctggatcc gcacgaagct cgagggtttc ggggacgaca cggcctcgat caaggccttc
      481 ggcctcgacg tcgtgacgga gctgtgcgat cgcctgctgc agggcggggc gccgggcctg
      541 cacttctaca ccatgaacat ggcgggcccc agctcgacca tctggcagcg cctcgggctg
      601 tagcgaaggg gctaaccgcg ggcgctacaa tcgctccaca ataatcaggg gagccgcgcc
      661 ttgaagaccg ccgccagtcc gcccagcgag gccgtgcccc agagctcgat gctcgcatcg
      721 cgaatccgct cgctgccgac cgacgaggcg gcggccctca tcgagcgcgc ggtgcccgga
      781 gagtcgatcg aggcgctgct gcaattgaat cccgcgatgg tgcaggcgat cctgcgcgag
      841 ctcgaatcgg ggccgcgtcg cttcatcacg gcttcggcgc ctcccgaggt cgcggcccag
      901 tggaagcgca acgcgagcta tccgagcgaa tccatcgggc agctgatgga gccgtcgctc
      961 tcggtcttca gccccgacat gaccgtgggc gaggccatcg aggcgctgcg cccgctcgtg
     1021 aggagcgcgt tcgtgaccta cgtgtacgtg gtcgactccg aggggaagct cggcggcctc
     1081 gtcaccatgc gcgacctgct cttcaacgat cgcggggcgc gcctcgccga cgtgatgctc
     1141 gccaatccct tctcgctcgc gccggagctg accgtggccg aggcgatgaa gctcacggcg
     1201 aaccggcact atccccagta tcccgtcacc gatgccgatg ggcggctcgt ggggctggtg
     1261 cgcggcgcgg cgctcttcga gctggaagcc ttcgagatca ccgcccaggc cggcgcgatg
     1321 gtcggcgtgg aaaaggagga gcgcgtcgcg acttccatcg cgcagagctt caagttccgc
     1381 aacccgtggc tgctcatcaa cctgctcacc gcgttcgccg cgggcggcgt ggtcgcgctc
     1441 ttccagggca cgctcgacaa gctcgtgatc ctcgcgacgt tcctcccggt gctcgcgggg
     1501 caggcgggca acaccggatg ccaggccctc gccgtcaccg tgcgcggcat cacgctgggc
     1561 gacatcaagc ccggaggcgg cggcgcgctc acctcgaagg aggcgctcct cggcttcgcc
     1621 aacggcgcgg tgacgggcgt gctgtgcggc atcgcgatgt acctgtatgc cctggcgcaa
     1681 agctcgcccc atgcaatcct cctcggcgtc gtggtgaccc tcgcgatggt cgggagctgc
     1741 tgcatcagcg ggatctccgg cgccctggtg ccgctcgcgc tgaagcgctt cggcgccgac
     1801 cccgccacgg cctcgagcat catcgtcacg acggcgaccg acgtgaccag catgggcctg
     1861 cttctcggcc tcgccgccgt gatgattcgc tgaggaaggg ttatcattcg cgcatgggac
     1921 tccggacggc cttcgccatc attctcgtcg cgctcgcttc tgccgccggc gcgcaggagc
     1981 aggcattcac caaccgctcg accgagctga aggatcgcgg cgcgagcgac gcgcgcaccc
     2041 tcgcgacgct tcccgagaac acttcggtga aggtgctcgc gcgcggcggc ggctggacgc
     2101 gcgtcgaggc tgccggccag agcggctggc tcaacgtctt ccacctgcgc tttgccgcgg
     2161 cggtcgatag ctcgggctct tcgggcggcg cgttcggggg cctgacgtcg ttcctcggcg
     2221 gcgggcgcaa cacgagccag aaagccaata tcgcgaccac cggcatccgc ggcctgtcgc
     2281 ccgaggacat caagaacgcg agccccgatc ccgcggcgct cgcgaaggcg cagtcttatc
     2341 gtgccgacaa gcccgccgcg gagcgcttcg ctcgcgaagc caagctcgcg gcggtttcga
     2401 tcgattacgt cggcgattcc gccaagggag gacgtcgatg aggttcgcat tgctcaccgc
     2461 gatcgcgctc gcgtgcactt gcgcgaacgc gcaattcggc agcctcctcg atctcggcaa
     2521 gaaggcgctc gacaagaagc agcagctcga gaccgccaac aaggaattca ccaccgacga
     2581 ggagatcgcg ctgggcgagg ggatcgcctc cgccttcctc ggcgcggcgc cgctgcatca
     2641 cgaccagaac ctgcagcgct acgtgaaccg cgtgggacgc tgggtcgcga tgcacggcga
     2701 gcggcccgac ctgccgtgga ccttcggcgt catcgatacc gaaacgatca atgcgttcgc
     2761 gtttccgggc ggcaccgtga tcgtctcgtc cgggctgctc aagaggctca acagcgagtc
     2821 ggagctcgcc ggcgccctcg cccacgaagt cgcccacgtc gtgaagcgcc accagatcca
     2881 ggcgatccag tcccaggcga gcacggattt ctgggcggga ctcggccagg acgtcgtctc
     2941 tgaccgtgtt gcacgcagcg gcggcggcgc ggtcacgcat atggtgaacc agaagctcgt
     3001 cgacatggga gcgaacctgg tgaagagcgg cttc
//
LOCUS       DASVII010000227         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_298403,
            whole genome shotgun sequence.
ACCESSION   DASVII010000227 DASVII010000000
VERSION     DASVII010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298403"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VF309_02920"
     CDS             <1..940
                     /locus_tag="VF309_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX7465566.1"
                     /translation="AVLYGVKPAVVAIVLAAAWRIGSRTLRNAALWAIAVAAFVSLFA
                     FSAPFPLVILVAAILGAAGGRIAPRLFALSKHGAGPATHASIIDDDTPTPAHARFSAA
                     KLAAIVAAFLALWAAALGALALALGWQDALTQMGWFFTKAALLTFGGAYAVLPYVLQG
                     AVEHYGWLTAPQMIDGLALGETTPGPLIMIVAFVGYLGAATAGAIVATFFTFLPSFLF
                     ILAGGPLVERTRDDVKLTAPLTAITAAVVGVIVNLAVFFGWHVFWPGGFDIAACAIAT
                     IATVVLVTNRAQVIPVIAASGAAGLALWALGLQGAV"
     gene            complement(925..1404)
                     /gene="moaC"
                     /locus_tag="VF309_02925"
     CDS             complement(925..1404)
                     /gene="moaC"
                     /locus_tag="VF309_02925"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX7465567.1"
                     /translation="MTEKLTHFDERGAARMVDVAGKSETHRVAVAAGRIRMLPATLAI
                     VLAGTAKKGDVLGVARIAAIQAAKRTSELIPLCHPVPLTSVAVDFTPDEKSSAIECRA
                     TVECTARTGVEMEALTAVQVGLLTIYDMCKAVDRGMTMEAIRLVEKRGGKSGTYTAP"
     gene            1438..>1645
                     /locus_tag="VF309_02930"
     CDS             1438..>1645
                     /locus_tag="VF309_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HEX7465568.1"
                     /translation="MTLRPVIASLLMALVALPAPAQTLPDLGDASSAALSDAQEKTIG
                     NRIMREVRIDPSVIDDPEITDYISS"
BASE COUNT          204 a          585 c          556 g          300 t
ORIGIN      
        1 ggccgtgctc tacggcgtga agcccgcggt ggtggcgatc gtgctcgcgg cggcgtggcg
       61 catcggatcg cgcacgctgc gtaacgccgc gctgtgggcg atcgcggtcg cggcgttcgt
      121 ctcgctcttc gctttctcgg cgcccttccc gctggtgatc ctggtggctg cgatcctcgg
      181 cgcggccgga gggcgcatcg cgccgcggct cttcgcgctt tcgaagcacg gggcgggacc
      241 ggccacgcac gcgtcgatca tcgatgacga cactcccacg ccggcgcacg cgcgcttttc
      301 cgcggcgaag cttgccgcga tcgtggccgc tttcctcgcg ctatgggccg cggcgctcgg
      361 cgcgctcgcc ctcgcgctcg gctggcagga cgccctcacg caaatgggct ggttcttcac
      421 caaggccgct ctcctcacct tcggcggtgc gtacgcggtg ctgccctacg tgctgcaggg
      481 cgccgtggag cactacggct ggcttaccgc gccgcagatg atcgacggcc tcgcgctggg
      541 cgagaccacg ccgggcccgc tcatcatgat cgtcgccttc gtgggctacc tgggcgcggc
      601 caccgcgggc gcgatcgtgg cgaccttctt cacgttcctg ccgtcgtttc tcttcatcct
      661 cgccggcggg ccgctggtgg agcgcacacg cgacgacgtg aagctcaccg ctccgctcac
      721 cgcgatcacc gcggcggtgg tgggcgtgat cgtgaacctc gcggtgttct tcgggtggca
      781 cgtgttctgg cccggaggct tcgacatcgc ggcatgcgcg atcgcgacga tcgccaccgt
      841 ggtgctggtc accaaccgcg cgcaggtgat cccggtgatc gccgcgagcg gcgcggccgg
      901 cctcgccctc tgggccctgg gacttcaggg cgccgtgtag gtcccggact tcccgccgcg
      961 cttttccacg aggcggatcg cctccatcgt catgccgcgg tccacggcct tgcacatgtc
     1021 gtagatggtg aggagcccca cctggacggc ggtgagcgcc tccatctcga cgccggtgcg
     1081 cgccgtgcat tccacggtag cgcggcattc gatcgcggag ctcttctcgt cgggcgtgaa
     1141 atccacggcc accgaggtga gcgggaccgg gtggcagagc gggatcagct cggaagtgcg
     1201 cttggcggcc tggatcgcgg cgatgcgcgc gacgcccagg acgtcgccct tcttcgcggt
     1261 cccggcgagg acgatggcga gcgtggcggg caacatgcgg atccttcccg cggccaccgc
     1321 cacgcgatgg gtctcggatt tgcccgcgac gtcaaccatc cgcgcggcgc cccgttcgtc
     1381 gaagtgggtg agtttttccg tcataattga tattagcagt tcgaaacctt cctcctagtg
     1441 accctacgcc ccgtcatcgc ctcgctcctg atggccctcg tggcgctccc cgcgcccgcg
     1501 cagaccttgc ccgatctggg cgacgcgtcc tcggcggcgc tctcggatgc gcaggaaaag
     1561 accatcggca accgcatcat gcgcgaggtg cgcatcgatc cgtcggtgat cgacgatccg
     1621 gagatcaccg actacatttc ctcga
//
LOCUS       DASVII010000228         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_82672, whole
            genome shotgun sequence.
ACCESSION   DASVII010000228 DASVII010000000
VERSION     DASVII010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82672"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VF309_02935"
     CDS             complement(<1..1085)
                     /locus_tag="VF309_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7465569.1"
                     /translation="MAWIALVGPEQEENLSLRYLAASLVEGGFESRIVVFNGEADFVS
                     AITAVTKAEEPPLLVGVSLAFQWRAPDFLALIVGLRETGYRGHVTVGGHFATFAAEDL
                     LRDFPELDSVCRQEAEETLVALGRALEAGAPWQDIPGVVSRDDAGEARFAPMPEVPEL
                     DRLPHPVRGEPARCFDHGIAPLVSSRGCYANCTFCCIAAWHEQTLPGKRYRLREPADV
                     AAEMAEMRRDQGVDIFVFHDDNFFIPNHGKSLARLHALADAIEAEGLRDFATVVKARP
                     NDVHPEVFRVLIERLGCIRCYVGIETDADQGLVTLRRWGKSSQNHRAIDVARSLDLFV
                     CFNILMFDPDTTLASVRKNLDFIEYAS"
     gene            1149..>1569
                     /locus_tag="VF309_02940"
     CDS             1149..>1569
                     /locus_tag="VF309_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465570.1"
                     /translation="MARILIIDDNETMREGLGATVRRMGHEAVLAPSGAEGLTHVTPA
                     GGRPGVDFVITDLKMDGTGGLEVVKQVRERDPDCPVMIVTAFGTVETAVEAMRLGAFD
                     FLQKPFAPEVVRLKVARAIELRDERRALERAQAENAAL"
BASE COUNT          253 a          554 c          543 g          219 t
ORIGIN      
        1 tcggaggcgt actcgatgaa atccaggttc ttgcgcacgc tcgccagcgt cgtatccgga
       61 tcgaacatga ggatgttgaa gcagacgaac aggtccagcg accgcgcgac gtcgatggcg
      121 cggtggttct ggctcgactt gccccacctc cgcagcgtca ccagcccctg atcggcgtcg
      181 gtctcgatgc cgacgtagca gcggatgcaa ccgagccgct cgatcagcac ccggaacacc
      241 tcggggtgga cgtcgttggg acgcgccttc accacggtcg cgaagtcgcg cagcccctcg
      301 gcctcgatgg cgtcggcgag cgcgtgcagg cgggccaggc tcttgccgtg gttggggatg
      361 aagaagttgt cgtcgtgaaa cacgaagatg tcgacgccct ggtcgcggcg catctcggcc
      421 atctccgccg cgacgtcggc cggctcgcgc aggcggtagc gctttccggg cagggtctgc
      481 tcgtgccagg cggcgatgca gcagaaggtg caattcgcgt agcagccgcg gctcgacacc
      541 agcggcgcga tgccgtggtc gaagcagcgc gcgggctcgc cgcgcacggg gtggggcaac
      601 cggtccagct cgggcacctc cggcatgggc gcgaaccgcg cctcccccgc gtcgtcgcgc
      661 gacacgacgc cggggatgtc ctgccagggg gcgcccgcct ccagcgcgcg gccgagggcg
      721 acgagggtct cctccgcctc ctggcggcaa acggagtcga gctcggggaa gtcgcgcagc
      781 aggtcctccg cggcgaaggt cgcgaagtgc ccaccgacgg tgacgtgccc ccggtagccg
      841 gtctcgcgca gcccgacgat caacgccagg aagtccggcg cccgccactg gaaggccagc
      901 gacacgccca ccagcaaggg cggctcctcg gccttcgtga cggccgtgat cgccgacacg
      961 aagtccgcct cgccattgaa cacgacgatg cggctttcga accctccctc caccaggctg
     1021 gcggcgaggt accggaggct caggttctct tcctgctcgg ggccaacgag cgcaatccac
     1081 gccatggcaa ccctccctca agtcaagcta tcgacttcac gatagcacga ggcgacaatc
     1141 ggaggcagat ggcccgcatc ctcatcatcg acgacaacga aacgatgcgc gagggcctcg
     1201 gcgcgacggt gcgccgcatg ggccacgagg cggtgctcgc cccctcgggc gccgaggggc
     1261 tcacccacgt cacccccgcg ggcgggcggc cgggcgtgga cttcgtcatc acggacctca
     1321 agatggacgg caccggcggc ctcgaggtgg tcaagcaggt gcgcgagcgc gatcccgact
     1381 gcccggtcat gatcgtcacc gccttcggca cggtcgagac ggcggtggag gcgatgcgcc
     1441 tcggcgcctt cgattttctg cagaagccct tcgcgcccga ggtagtccgg ttgaaggtcg
     1501 cgcgcgccat cgagctgcgc gacgagcgcc gcgccctcga gcgcgcccag gccgagaacg
     1561 cggccctgc
//
LOCUS       DASVII010000229         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_442268,
            whole genome shotgun sequence.
ACCESSION   DASVII010000229 DASVII010000000
VERSION     DASVII010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442268"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF309_02945"
     CDS             complement(<1..336)
                     /locus_tag="VF309_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465571.1"
                     /translation="MPEAGRPLLEVRGLSRDFDVSRPWLNRVIEGTSRQILRAVDGVS
                     FTINRGETLALVGESGCGKSTVARLICGLYAPSAGSILFDGKDLAKIADRTEMLAMRR
                     RFQMIFQDPY"
     gene            complement(329..1366)
                     /locus_tag="VF309_02950"
     CDS             complement(329..1366)
                     /locus_tag="VF309_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465572.1"
                     /translation="MPAATPDAKPLLQVKNLRVEFNTRRGTLVAIDDVSFDIAPGEVL
                     GVVGESGAGKSITGTAIIGLLEPPGRIAGGEILLEGMRIDNLPAEEMRKIRGRRIGAI
                     FQDPLTSLNPLYTIGHQLTETIQTHLAMSMAQARARAIELLAEVGIPAPERRIDHYPH
                     QFSGGMRQRVVIALALCANPQLIIADEPTTALDVSIQAQIITLLKRLCRDNGTAVMLV
                     THDMGVIAETADRVAVMYAGRIAEIGPVQEVIHNPQHPYTAGLMASIPALGQEVERLS
                     QIEGSMPRLNAIPTGCAFNPRCPRVFGRCTVERPSLLPAGKSLAACWLHATGSAPLDL
                     NKDWSEGAVHA"
     gene            complement(1367..>1612)
                     /locus_tag="VF309_02955"
     CDS             complement(1367..>1612)
                     /locus_tag="VF309_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465573.1"
                     /translation="VDDVNSMNNRSVITPGLHKVTIDLPPRKGFPATQATFDLETKPC
                     VRYYVAAELKSETLQEWAPVVRHEDPIGECRKKFGVS"
BASE COUNT          242 a          532 c          570 g          268 t
ORIGIN      
        1 gtacggatcc tggaagatca tctggaagcg ccttcgcatc gcgagcatct cggtgcgatc
       61 cgcgatcttc gcgaggtcct tgccgtcgaa gaggatcgat cccgcgctcg gcgcgtaaag
      121 gccgcagatg aggcgcgcaa cggtggactt gccgcagccc gattcgccga cgagcgcgag
      181 cgtctcgccg cggttgatgg tgaacgagac gccgtcgacc gcgcgcagga tctgccgcga
      241 cgtgccctcg atcacgcgat tgagccatgg gcgcgagacg tcgaagtcgc gcgacaggcc
      301 gcgcacctcg aggagcggtc gcccggcctc aggcatgcac cgccccctcg ctccaatcct
      361 tgttcaggtc gaggggggcc gatcccgtcg cgtgcagcca gcaagccgcg agggattttc
      421 ccgcgggaag aaggctcggg cgctcgaccg tgcagcgacc gaagacgcgc gggcaccgcg
      481 gattgaaggc gcaccccgtc ggaatcgcgt tgaggcgcgg catggagccc tcgatctgcg
      541 agaggcgctc gacctcctgg cccagcgccg gaatggacgc catgaggccc gccgtgtagg
      601 ggtgctgcgg attgtggatc acttcctgga cggggccgat ctccgcgatg cgtcccgcgt
      661 acatcaccgc gacgcgatcg gcggtctccg cgatgacccc catgtcgtgg gtcacgagca
      721 tcaccgccgt gccgttgtcg cggcacaggc gcttcagcag cgtgatgatc tgcgcctgga
      781 tcgagacgtc gagggcggtg gtgggctcgt cggcgatgat gagctgcgga ttcgcgcaga
      841 gcgccagggc gatcacgacg cgctggcgca tcccgcctga gaactggtgc gggtagtgat
      901 cgatgcgccg ctccggcgcc ggaatgccca cctcggcgag gagctcgatc gccctggcgc
      961 gagcttgggc catgctcatg gccaggtgcg tctggatggt ctccgtgagc tggtgcccga
     1021 tcgtgtagag cggattcaac gacgtgagcg gatcctggaa gatcgcgccg atgcgccggc
     1081 cgcggatctt gcgcatctcc tccgcgggga ggttgtcgat gcgcatgccc tcgaggagga
     1141 tctcgccgcc cgcgattcgc cccgggggct cgagaagccc gatgatcgcg gtgccggtga
     1201 tcgacttgcc cgcgcccgac tcccccacca cgcccagcac ctcgccgggc gcgatgtcga
     1261 agctcacgtc gtcgatggcg acgagcgttc cccggcgcgt gttgaattcc acgcgcaggt
     1321 tcttcacctg caggagcggc ttcgcgtcgg gagtcgccgc gggcatctag ctcacgccga
     1381 acttcttgcg gcactcgccg atcggatcct cgtggcggac gacgggcgcc cactcctgca
     1441 gggtctccga cttgagctcg gcggcgacgt agtagcgcac gcacggcttg gtctcgaggt
     1501 cgaaggtcgc ctgcgtcgcg gggaagccct tgcgcggcgg caggtcgatg gtcaccttgt
     1561 gcagcccggg cgtgatcacc gagcggttgt tcatcgagtt gacgtcgtcg ac
//
LOCUS       DASVII010000230         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_226770,
            whole genome shotgun sequence.
ACCESSION   DASVII010000230 DASVII010000000
VERSION     DASVII010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226770"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VF309_02960"
     CDS             complement(<1..247)
                     /locus_tag="VF309_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7465574.1"
                     /translation="MKHRNFFVERPIFSGVIAIIITLAGLAASQVLGVAQYPEIAPPT
                     VTITANYPGASAETLARTVAAPIEEQLSGIENLIYFNS"
     gene            complement(244..1368)
                     /locus_tag="VF309_02965"
     CDS             complement(244..1368)
                     /locus_tag="VF309_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7465575.1"
                     /translation="MKPFPGLRPLVAAVAVSLAALAGCNTKGVAPAAAPAAVPARVLE
                     VQPRSVPIVVEGVGQVEGSKEVEVRAKVSGTLKKTLYKEGEVVTPGTTLFQIDPEPFE
                     IALVQARSQLAQEAARNEQMRREAARMKELIGQRAISQKELDDAQSNAKLSDASLQSA
                     QSRVKEAELNLSYTKVDAPVGGVTGRSQHSEGSLISTSGADGLLTTLSQMDPVWVRFS
                     LSESDLAKLPGGRLTRNSNPDIRLVLPDGSIYPVTGRLNFTASQIDPKLATMQLRAEF
                     PNGKSGLLPGQFVRVQLVAGKRDNVFLVPQIAVTQTETGYLLFVLDAEGKAQLRPVKV
                     GDWVGKDWTILEGLAKGDKVVVDNLMKLRPGAAVTPVTGS"
     gene            1447..2061
                     /locus_tag="VF309_02970"
     CDS             1447..2061
                     /locus_tag="VF309_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX7465576.1"
                     /translation="MRRSKEDSLKTRRSIVLAARRVFARRGVSQTSMEQIACAAGVTR
                     GAVYGHFKDKGALFQTMREQVRLPLMDVLNEAMLHSPGDPLAGVERYLLAVVEAVRND
                     PATRDTFQILIFKCEYVGDFAADIKRQSVRLAELAGELDRVYRAAARLGQLRKGVVPR
                     LAALETCAFLMGTMRLWLLDRDGTLVRGDAAKLMRTHMANLRAA"
BASE COUNT          334 a          712 c          770 g          356 t
ORIGIN      
        1 tcgagttgaa gtagatgagg ttctcgatgc cggacagctg ctcctcgatg ggagccgcca
       61 cggtgcgcgc gagggtctcg gcgctcgcgc ccggatagtt cgccgtgatg gtcacggtgg
      121 gaggcgcgat ctcggggtac tgcgccacgc ccagcacctg ggacgccgca aggccggcaa
      181 gcgtgatgat gatcgcgatc acgcccgaga agatcgggcg ctcgacgaag aagttgcggt
      241 gcttcatgag ccggtcacgg gcgtcaccgc cgcgcccggc cgcagcttca tgaggttgtc
      301 gacgacgacc ttgtcgccct tggcgaggcc ttcgaggatc gtccagtcct tgccgaccca
      361 gtcgcccacc ttcacggggc gaagctgcgc cttgccctcg gcgtcgagca cgaagagcag
      421 gtagccggtc tcggtctggg tgacggcgat ctgcggcacg agaaagacgt tgtcgcgctt
      481 gccggcgacg agctgcacgc ggacgaactg ccccgggagc aggcccgact tcccgttggg
      541 gaactcggcg cgaagctgca tcgtcgccag cttcggatcg atctgcgagg cggtgaagtt
      601 gagccggccc gtgaccgggt agatcgaacc gtcggggagc accaggcgga tgtcgggatt
      661 ggagttgcgc gtgaggcgcc cgcccggaag cttcgcgaga tccgattcgg agaggctgaa
      721 gcgcacccac acggggtcca tctggctcag cgtcgtgagc aggccgtccg ccccgctggt
      781 ggagatgagg ctgccctcgg aatgctgcga gcggccggtc acgcccccca cgggcgcgtc
      841 aaccttcgta taggagaggt tgagctcggc ttccttcacg cgggactgcg ccgattgcag
      901 cgacgcgtcg gagagcttcg cgttggactg cgcgtcgtcg agctccttct ggctgatggc
      961 gcgctgcccg atgagctcct tcatgcgcgc cgcctcgcgg cgcatctgct cgttgcgcgc
     1021 cgcttcctgc gcgagctgcg agcgcgcctg gacgagcgcg atctcgaaag gctccgggtc
     1081 gatctggaag agcgtggttc ccggggttac cacctcgcct tccttgtaga gggtcttctt
     1141 gagggtgccg gaaaccttgg cgcggacctc gacttccttc gagccctcga cctgccccac
     1201 gccctcgacc acgatgggga cgctgcgcgg ctgcacctcg aggaccctgg cggggacggc
     1261 ggcgggggcc gcggcgggcg ccacgccctt ggtattgcag ccggcgagcg cggcgagcga
     1321 gacggcaacg gcggcgacga gcgggcgaag cccgggaaaa ggtttcatgg gatggaacgc
     1381 gatcgcctct gctggggaag acgattatat atacaatcat gactgtatcc aaatatgggg
     1441 ccggccatgc gccgctcgaa ggaagactcc ctcaagacgc gccgcagcat cgtgctcgcc
     1501 gcgcgccgcg tgttcgccag gcgcggcgtt tcgcagacga gcatggagca gatcgcgtgc
     1561 gccgcggggg tgacgcgcgg ggcggtctac ggccacttca aggacaaggg cgccctcttc
     1621 cagacgatgc gcgagcaggt tcgcctgccg ctgatggacg tgctgaacga ggcgatgctc
     1681 cactctcccg gggacccgct cgcaggcgtc gagcgctacc tcctggcggt ggtcgaggcg
     1741 gttcgcaacg accccgcgac gcgcgacacc ttccagatcc tcatcttcaa gtgcgaatac
     1801 gtgggcgact tcgcggccga catcaagcgc cagtccgtgc gcctcgccga gctcgccggg
     1861 gagctcgacc gcgtctaccg cgccgccgcg cgcctcgggc agctgcgcaa gggagtcgtg
     1921 ccccgcctcg cggcgctcga gacctgcgcc ttcctcatgg gaacgatgcg cctgtggctc
     1981 ctcgacaggg acggcacgct ggttcgcggg gacgcggcga agctcatgcg cacgcacatg
     2041 gcgaacctcc gcgcggcata ggcgcgaagt ggcgttgatc gcatccctgg gcatcgccca
     2101 gatcatttcg tggggttcgc tctactactc gatcggcgtg ctcgggcccg cgatgcgcgg
     2161 cgacctggga gt
//
LOCUS       DASVII010000231         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_694153,
            whole genome shotgun sequence.
ACCESSION   DASVII010000231 DASVII010000000
VERSION     DASVII010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694153"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VF309_02975"
     CDS             <1..1472
                     /locus_tag="VF309_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7465577.1"
                     /translation="PVGYLYTIGNPRTDWCFRTESEPGLNGRSLGYARGKVLGGCSSI
                     NAMVYMRGQKVDYDHWESLGNRGWSWADVLPYFKRAERHFGGANEHHGGDGELFVEEP
                     RVKWEILDAWRDAAAECGIPKVEEFNRGDNFGNAYFHMNQRGGRRWSATNAFLRPALG
                     RANLTLITRAHTRKVLVDTRDGVKRSTGIELVVEGRGEGVARARKAVILAAGSIGSPQ
                     ILQLSGIGPSNLLQQFSIPVVHESPGVGDNLHDHLQIRMVWKVKGPTLNERSNRWLGK
                     VAMGLEYLLFKTGPLTMPPSQLGAFAKSDPREATPDVEWHVQPLSLEKFGDPLHSFPA
                     ITPSVCNLRPTSRGAVRIRSGDPMAAPEIRLNYLSTPEDQAVAARSMRMTRRIMESAA
                     LARYSPEEWRPGAAAQSDAKLVEAAGDLGTTIFHPVGTCRMGRDERAVVDDRLRVHGI
                     EGLRVVDASIMPRITSGNTNAPTYMIAERGAELILADSR"
BASE COUNT          252 a          536 c          525 g          237 t
ORIGIN      
        1 ttcccgtggg ttacctctac accatcggca acccgcgcac cgactggtgc tttcggacgg
       61 agagcgagcc gggcctcaac ggccgcagcc tcggctacgc gcgcggcaag gtgctcggcg
      121 gatgctcctc gatcaacgcg atggtgtaca tgcgcgggca gaaggtcgac tacgaccatt
      181 gggagtcgct cggcaatcgc ggctggtcgt gggcggatgt cctgccgtac ttcaagcgcg
      241 cggagcggca tttcggcggg gccaacgagc accacggcgg cgacggcgag ctcttcgtgg
      301 aggagccgcg cgtgaagtgg gaaatcctcg acgcatggcg cgacgcggcc gcggagtgcg
      361 gcattcccaa ggtcgaggaa ttcaatcgcg gcgacaactt cggcaacgcg tatttccaca
      421 tgaaccagcg gggcgggcgc cgctggagcg cgaccaacgc cttcctgcgc ccggccctcg
      481 ggcgcgcgaa cctcaccctc atcacgaggg cgcacacccg gaaggtcctg gtcgacaccc
      541 gggatggcgt gaagcggtcc acgggcatcg agctcgtcgt ggaaggccgg ggcgagggcg
      601 tcgcccgggc gcgcaaggcg gtgatcctcg ccgcgggctc gatcggctcg ccgcagatcc
      661 tgcagctctc cggcatcggg ccgagcaatt tgttacaaca attctcgatc cccgtcgtcc
      721 acgagtctcc gggcgtcggc gacaatcttc acgaccactt gcagatccgg atggtgtgga
      781 aggtgaaggg cccgacactg aacgagcgct ccaatcgctg gctcggcaag gtcgcgatgg
      841 ggctcgaata cctgctcttc aagacgggac cgctcacgat gccgccctcg cagctcggcg
      901 ccttcgcgaa gagcgacccg cgcgaagcca cgcccgacgt cgagtggcac gtgcagccgc
      961 tgtcactcga gaaattcggc gatccgctgc attcttttcc ggcgatcacg ccttcggtgt
     1021 gcaacctgcg cccgacttcg cgcggagccg tgcgcattcg ctcaggcgat cccatggccg
     1081 cgcccgagat ccgcctgaac tacctctcga ctcccgagga ccaggccgtc gccgcgcgct
     1141 cgatgcgcat gacgcgtcgc atcatggagt cggcggcgct cgcgcgctat tcccccgagg
     1201 agtggcggcc cggcgccgcc gcgcagtccg acgcgaagct ggtcgaggcc gcgggcgatc
     1261 tcggaacgac gatcttccac ccggtcggaa cctgcaggat ggggcgcgac gagcgggcgg
     1321 tcgtcgacga tcgactgcgc gtgcacggga tcgagggcct tcgagtggtc gatgcatcca
     1381 tcatgccgcg catcacctcg ggcaatacga acgcgccgac gtacatgatc gcggagcgcg
     1441 gcgccgagct catcctggcc gacagccggt gaagcatcgc gctcccgccc tcatcctcct
     1501 cgccgtggcg acgctcgccg gcgcgtggct gctggtgcgc ccaccggagc
//
LOCUS       DASVII010000232         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_514220,
            whole genome shotgun sequence.
ACCESSION   DASVII010000232 DASVII010000000
VERSION     DASVII010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514220"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF309_02980"
     CDS             complement(<1..359)
                     /locus_tag="VF309_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465578.1"
                     /translation="MTPHSDAEDDLDFTAPQKATPPPPPEKVASARKEASTGSPQLQK
                     TGFYVAMARNAGGRVTPRNGSRHSVFIIEDDKALTELVGEVLAKSGFLTRFARNRTEI
                     NAEFNKPPLPDVVLLDV"
     gene            complement(378..986)
                     /gene="plsY"
                     /locus_tag="VF309_02985"
     CDS             complement(378..986)
                     /gene="plsY"
                     /locus_tag="VF309_02985"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7465579.1"
                     /translation="MTAFLYALGGYLAGSIPFGVIVSRVMAVPDPRTYGSGNIGATNV
                     LRSGHRVAALLTLMGDAGKAWAALLVARIAGASVELLAIVAIAAFLGHLFPVWLGFRG
                     GKGVATAAGVLIALDWRLGLAVIVAWLIIAVATRYSSLAAIGAALVAPAAAWYLAGLG
                     PVLGAVVAISVVLVARHRDNIGKLLRGEESRIGEKTTAASTN"
     gene            1011..1373
                     /locus_tag="VF309_02990"
     CDS             1011..1373
                     /locus_tag="VF309_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX7465580.1"
                     /translation="MDTIFIREFRVDAWVGIYEWEKLRAQTLEMDIEIGIPGNAVGRT
                     DNIHDTVHYGDVVERIAAELAARKFKLLEALAEHVCAIVTSEFKAPWVRLSVAKLGHI
                     RNVRKVGVVLERRRDPGE"
     gene            1380..1574
                     /locus_tag="VF309_02995"
     CDS             1380..1574
                     /locus_tag="VF309_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX7465581.1"
                     /translation="MLKWLLVLAVGVAVIALATPWLQRHGLGRLPGDVTINWRGRRVY
                     VPITTTILLSLALTLLARLV"
     gene            complement(1571..>1752)
                     /locus_tag="VF309_03000"
     CDS             complement(1571..>1752)
                     /locus_tag="VF309_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465582.1"
                     /translation="RNFIPFLLYGIVMLVLCVAAVIPIGLGLLVWIPLSVTSTYAAYR
                     DIFTKDSRPAASVAV"
BASE COUNT          301 a          607 c          574 g          270 t
ORIGIN      
        1 tgcacatcga ggagcaccac gtccggaagc ggcggcttgt tgaactccgc gttgatctcg
       61 gtgcggttgc gcgcgaagcg cgtgaggaat ccggatttcg caagcacctc gcccaccagc
      121 tccgtcagcg ccttgtcgtc ctcgatgatg aagaccgaat ggcgcgagcc gttgcgcggc
      181 gtgacgcgcc cgccggcgtt gcgcgccatg gcgacgtaga agccggtctt ctggagctgg
      241 gggctcccgg tgctggcctc cttgcgcgcg ctcgcgacct tctccggcgg cggaggagga
      301 gtcgccttct gcggggccgt gaagtcgagg tcgtcctcgg cgtcggaatg gggagtcatt
      361 tacgaggtac ctcttcttca gttggtgctt gccgccgtcg tcttctcgcc gatcctgctt
      421 tcctcgccgc gcagcagctt gccgatgtta tcgcggtgcc tcgcgacgag caccacgctt
      481 atcgccacca ccgcgccgag caccgggccc aagccggcca ggtaccacgc cgccgccgga
      541 gcgacgagcg ccgcgccgat cgccgccagc gaagaatacc gcgtggcaac cgcgattatc
      601 agccacgcga cgatcaccgc caagccaagc ctccagtcga gcgcgatcag caccccggcg
      661 gccgtcgcga cgcccttgcc tccgcgaaag cccagccaca cgggaaatag gtgcccgagg
      721 aaggcggcga tcgcgacgat cgcgagcaac tcgaccgagg cgccggcaat ccttgccacg
      781 agcaacgccg cccacgcctt gccggcatcg cccatcagcg tgagcagcgc cgcgacacgg
      841 tggcccgagc gcagcacgtt ggtcgcgccg atattccccg agccgtaggt tcgcgggtcc
      901 ggcaccgcca tcacgcggct cacgatcacg ccgaacggga tcgagccggc gagatatccg
      961 cccagcgcgt acaggaacgc ggtcatggtc atactctaca atacgaggcc atggacacga
     1021 ttttcatccg cgaattccgc gtcgacgcct gggtgggaat ctacgagtgg gaaaagctgc
     1081 gcgcgcagac gctcgagatg gacatcgaga tcgggattcc gggcaacgcc gtgggccgca
     1141 ccgacaacat ccacgacacg gtccactacg gcgacgtcgt cgagcgcatc gccgccgagc
     1201 tcgccgcgcg caagttcaag ctgctcgagg cgctcgccga gcacgtgtgc gcgatcgtca
     1261 cgagcgaatt caaggccccg tgggtgcggc tctccgtcgc gaagctgggc cacatccgca
     1321 acgtgcgcaa ggtgggcgtc gtcctcgagc ggcgccgcga tcccggcgaa tagcgcgcga
     1381 tgctcaagtg gttgctggtg ctggcggtcg gcgtcgcggt tatcgcgctc gcgaccccgt
     1441 ggcttcagcg ccatggcctg ggacggcttc cgggagacgt caccatcaac tggcgcggcc
     1501 ggcgcgtcta cgtgccgatc accaccacga tcctgctgtc cctcgcactg accctcctcg
     1561 cgcgcctcgt ctagacggcg accgacgcgg ccgggcgcga atccttggtg aagatgtcgc
     1621 gatacgccgc gtaggtgctg gtgacgctga gcgggatcca cacgaggagc ccgaggccga
     1681 tcgggatcac ggcggcgacg cacagcacca gcatcacgat tccatagagc aggaacggga
     1741 tgaaatttcg ca
//
LOCUS       DASVII010000233         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_11438, whole
            genome shotgun sequence.
ACCESSION   DASVII010000233 DASVII010000000
VERSION     DASVII010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11438"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF309_03005"
     CDS             <1..606
                     /locus_tag="VF309_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX7465583.1"
                     /translation="IVEATDVPVSADLEKGFGDSPEDAAETIREAAKVGLVGGSIEDA
                     SGDERQPIYALAHATERIAAAAEAAHALAYPFLLTARAENFLHGKTDLDDTIRRLQAF
                     EKAGADVLFAPALPDLAAVQAVCAAVTKPVNFMVAIRGKSWSVAELEAAGVRRISLAS
                     SLWRAAMTGLVEAGREVREKGTFGYLDHALPTAEVSRFMRD"
     gene            632..>2134
                     /gene="alaS"
                     /locus_tag="VF309_03010"
     CDS             632..>2134
                     /gene="alaS"
                     /locus_tag="VF309_03010"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7465584.1"
                     /translation="MQTRDIRRKFIDYFVSKGHTHVPSSPLVPANDATLLFTNAGMVQ
                     FKDVFLGLEKRPYNRAVTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDGIKYAWELLTVEYGLPKEKLWTTVYLTDDEAYDIWTKEIGVPPERCIRIGDKPGGR
                     KFESDNFWQMADTGPCGPCSEIFYDHGPEVEGGPPGSPDAEGDRYIEIWNLVFMQFNR
                     DEAGTMHPLPKPSVDTGMGLERLTAVLQGVHSNYEIDLFQSLIKAAARATGTKDLDSP
                     SLRVIADHIRACAFLICDGVIPMNEGRGYVLRRIARRAMRHGYKLGQKKPFFYTLVDD
                     LAKEMGEAYPELVKAEGRVTATLKQEEERFAETLETGMAILEGALAKNAKRLDGEVAF
                     KLYDTFGFPVDMVADIGRERGFAIDMAGFDAAMAAQQERSRSASKFKAGAQLEYNGAK
                     TTFRGYDTLGEEGRVVALYRGGSPVKSLSAGEEGIVVLDRTPFYAESGGQV"
BASE COUNT          376 a          704 c          729 g          325 t
ORIGIN      
        1 atcgtggaag cgaccgatgt cccggtctcg gccgatctgg agaagggatt cggcgattcg
       61 cccgaggatg cagccgaaac gattcgcgag gccgcgaagg tggggctggt gggcggctcg
      121 atcgaggatg cgagcggcga cgagcgccag ccgatctacg cgctcgccca cgcgaccgag
      181 cgcatcgccg cggcggcgga agccgcgcac gcgctcgcgt acccgttcct cctcacggcg
      241 cgcgcggaga acttcctcca cggcaagacg gacctcgacg atacgattcg tcggctgcag
      301 gcgttcgaga aggcgggcgc cgacgtcctg ttcgcgccgg cgcttcccga tctcgcggcc
      361 gtgcaggcgg tctgtgcggc ggtcacgaag cccgtcaact tcatggtggc cattcgtgga
      421 aaatcctgga gcgtggcgga gctcgaagcg gcgggcgtgc gccgcatcag cctcgcttca
      481 tcgctctggc gcgcggcgat gacgggactc gtcgaggcgg gccgcgaggt ccgcgagaag
      541 gggaccttcg gctatctcga ccacgcgctt cccacggccg aggtgagccg cttcatgcgg
      601 gattgaactc ggcgtttggt atccttaggc gatgcaaacc agggatatcc gcaggaagtt
      661 catcgactac ttcgtatcga agggccacac gcacgtgccc tcgagcccgc tcgtcccggc
      721 gaacgatgcg acgctcctct tcaccaacgc gggcatggtg cagttcaagg acgtgttcct
      781 cgggctcgag aagcgtccct acaaccgcgc ggtgacggcg cagcgttgcg tgcgcgccgg
      841 cggcaagcac aacgacctcg agaacgtggg ctacacggcg cgccaccaca cgttcttcga
      901 gatgctcggc aacttcagct tcggcgacta cttcaagcgc gacggcatca agtacgcgtg
      961 ggagctgctc acggtcgagt acgggctgcc aaaggaaaag ctctggacca ccgtctacct
     1021 gaccgacgac gaggcctacg acatctggac gaaggagatc ggcgttcctc ccgagcgctg
     1081 cattcgcatc ggcgacaagc cgggcgggcg caagttcgag agcgataact tctggcagat
     1141 ggccgacacc ggcccgtgcg gcccgtgctc ggaaatcttc tacgaccatg gccccgaggt
     1201 cgaaggaggc cccccgggct cgcccgatgc ggagggcgat cgctacatcg agatctggaa
     1261 cctcgtcttc atgcagttca accgcgacga ggcgggcacg atgcacccgc tgcccaagcc
     1321 gtccgtggac acgggcatgg gcctcgagcg cctgaccgcg gtgctgcagg gcgtgcattc
     1381 caactacgag atcgatctct tccagtcgct catcaaggcg gcggcgcgcg ccacgggcac
     1441 gaaggatctc gattcgccgt cgctgcgcgt catcgcggac cacattcgcg cgtgcgcctt
     1501 cctcatctgc gacggcgtga tcccgatgaa cgagggccgc ggctacgtgc tgcggcgcat
     1561 cgcgcggcgc gccatgcgcc acggctacaa gctcggccag aagaagccct tcttctacac
     1621 cctcgtggac gacctcgcga aggaaatggg cgaggcatac cccgagctcg tgaaggccga
     1681 agggcgcgtc accgccacct tgaagcagga ggaggagcgc ttcgcggaga cgctcgagac
     1741 cggcatggcg atcctcgagg gcgccctcgc gaagaacgcg aagcgcctcg acggcgaggt
     1801 cgcgttcaag ctctacgaca cgttcggctt tccggtggac atggtggccg acatcggccg
     1861 cgagcgcggc ttcgcgatcg acatggcggg cttcgacgcc gcgatggccg cgcagcagga
     1921 gcgctcgcgc tcggcgagca agttcaaggc cggggcgcag ctcgagtaca acggcgccaa
     1981 gaccacgttc cgcggctacg acacgctggg cgaggaaggg cgcgtcgtgg cgctctatcg
     2041 cggtggcagc cccgtgaaga gcctctccgc gggcgaggag ggcatcgtgg tcctcgatcg
     2101 cacgccgttc tacgcggagt ccggcggaca ggtc
//
LOCUS       DASVII010000234         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_550766,
            whole genome shotgun sequence.
ACCESSION   DASVII010000234 DASVII010000000
VERSION     DASVII010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550766"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VF309_03015"
     CDS             <1..989
                     /locus_tag="VF309_03015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX7465585.1"
                     /translation="AGTSAEAAAAAAASSVLVKLFPGQAQPVEAAYAASLAAIADGEG
                     KTAGVSLGRSVAVACLSMRDKDGTGVTGSYRPRTAPGVYVMTMMPAATEWPKVKPWFM
                     KSGAQFRPGPPPPLASAQWARDYEEIRVVGERQSPARSAQQTEVARFWTVVGAPSWNP
                     VVRSLALSSHLSLVENARLFALVNMAASDAFVAVFDAKYAHHFWRPITAIRNGDLDGN
                     DATTLDADWVPLVDTPPHPEYPCAHCITAGAIGEVLESQFGKGEVPAITMTSPTAPGA
                     THRWTRISDYVQEVDNARVWGGIHYRNSTEVGEAMGRRIGALAVRDFLRPVE"
     gene            1088..1819
                     /locus_tag="VF309_03020"
     CDS             1088..1819
                     /locus_tag="VF309_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7465586.1"
                     /translation="MQNEETYVSIKVWDLPVRIFHWAIFLLLAFQLVSGKMGGSMMAW
                     HAYSGYGMLVLVIFRVLWGFAGSTHARFASFLAGPAKTLRFARRLFSREAVPQVGHNP
                     LGGWMVIALLASLALQAMSGLFANDGAEFAGPLAVTAGFDLSTALTHFHRWNLRVLLV
                     LVGLHIAAVFFHLLVKREDLLGTMFTGMKRVPEAAVHERREGLRATPPRRVASRESAA
                     AHFVSPWRALALFVLALVLVSFVVR"
BASE COUNT          343 a          705 c          730 g          342 t
ORIGIN      
        1 ccgccggaac atcggcggaa gcggccgccg ccgcggccgc atcgtcggtg ctcgtgaagc
       61 tcttccccgg ccaggcgcag ccggtggagg ccgcctacgc ggcgagcctc gccgcgattg
      121 ccgatggcga ggggaagacc gcgggagtct cgctcggcag gagcgtggcg gtggcgtgcc
      181 tgtccatgcg cgacaaggac gggaccgggg tgaccggcag ctatcggccg aggaccgcgc
      241 ccggcgtgta cgtgatgacc atgatgccgg ccgcgaccga atggccgaag gtgaaaccct
      301 ggttcatgaa gtcgggcgcg cagtttcgcc ccggcccgcc gccgcctctg gcgagcgcgc
      361 aatgggcacg cgactacgag gagatcaggg tcgtcggcga gaggcaaagc ccggcacgca
      421 gcgcgcagca gaccgaagtc gcgcgtttct ggaccgtggt cggcgcgccc agctggaacc
      481 cggtggtgcg ctcgctggcg ctctcgagcc atctcagcct ggtcgagaac gcgcggctct
      541 tcgcgctcgt caacatggcc gcgtcggacg ctttcgtcgc ggtgttcgac gcgaaatacg
      601 cgcatcactt ctggcgcccg atcaccgcga tccgcaacgg cgacctcgac ggcaacgacg
      661 cgacgacgct ggacgccgat tgggtgccac tcgtggatac ccctccgcat ccggaatacc
      721 cgtgcgcgca ctgcatcacg gcaggcgcga tcggcgaggt gctcgagtcg caattcggca
      781 aaggcgaggt gcccgccatc acgatgacga gcccgacggc gcccggagcc acgcatcgct
      841 ggacgcgcat ctccgattac gtgcaggagg tcgacaatgc ccgcgtgtgg ggcggaatcc
      901 actaccgcaa ttcgaccgag gtcggcgaag cgatggggcg caggatcggc gcgctggcgg
      961 tgcgggactt cctgaggccg gtcgagtagc aaacgacgca ctcccggagg ttcccgggtg
     1021 cccggcgcgg aaggcacgct agaatgccgg acccctagcg ctcgccgcct tcggcacgaa
     1081 cggagcaatg cagaacgagg agacgtatgt ctcgatcaag gtctgggacc tgcccgttcg
     1141 catcttccac tgggccatat tcctgttgct cgccttccag ctggtgagcg gcaagatggg
     1201 cggcagcatg atggcctggc atgcctactc cgggtacggc atgctggtgc tggtcatctt
     1261 ccgcgttctc tggggcttcg ccggcagcac gcatgcgcgc ttcgcgagct ttctcgccgg
     1321 ccccgccaag accctgcggt tcgccaggcg cctcttctcg cgcgaggcag tgccgcaggt
     1381 gggccacaac ccgctcggcg gctggatggt gatcgccctc ctcgcgtcgc tcgcgctgca
     1441 ggcgatgtcg ggcctcttcg cgaacgacgg cgcggaattc gcggggccgc tcgcggtgac
     1501 ggccggattc gacctgtcga ccgccctcac gcacttccat cgctggaacc tcagggtcct
     1561 tctcgtcctc gtgggactgc acatcgctgc cgtgttcttc catttgctgg tgaaacggga
     1621 ggatctcctg ggcacgatgt tcacggggat gaagcgggtc ccggaagccg ccgttcacga
     1681 gcggcgcgag ggcctgcgcg ccaccccgcc ccgccgggtc gcctcgcggg aaagcgcggc
     1741 ggcccatttc gtgagcccct ggagagcgct cgccttgttc gtgctggcgc tcgtccttgt
     1801 ttcatttgtc gtgcgatgac catccccgaa tgtgtcgcag ttcataggag gcgcaagggg
     1861 gcaaaactag agtgcggtga ccaaggggag ggaccggaat gacatcgtgg ctgcgaagca
     1921 agcaggcgta tgcgcgcggc gcgcatttgc tgctgttggc catcggcctc ttctccttcc
     1981 acacggcctt cgcgaatccg ccggaggcca agtacgtcgg cgagaagacc tgcatcaagt
     2041 gccacgacgt ggaggccaag cacttcgggc acacgcagca cgcgaaggcc ttccggcaga
     2101 acccgaaaaa cgagctcgag
//
LOCUS       DASVII010000235         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_622398,
            whole genome shotgun sequence.
ACCESSION   DASVII010000235 DASVII010000000
VERSION     DASVII010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622398"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VF309_03025"
     CDS             <1..546
                     /locus_tag="VF309_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7465587.1"
                     /translation="EARDYGNDMRATIEAIDRCRHPVVAQIHGICVGGGLEIAGLADI
                     RICGKSSRFGVPINKLGLVMAYAEIGSLIALAGEATALEILLEGRVFDAKEAKEKGLV
                     TRVVPDAEVETEARATAQRIADGAPLVARWHKKFARRLRDPRPLTPEEYDEGFACFGT
                     EDFRIGYRAFLAKAKPAFEGK"
     gene            543..1745
                     /locus_tag="VF309_03030"
     CDS             543..1745
                     /locus_tag="VF309_03030"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7465588.1"
                     /translation="MNTGALKGIKVIELAQIMAGPTCGMLLADMGADVIKVEKLPGGD
                     DTRSYTEPSIRGESAAFMILNRNKRGIAVNLKKPGGLEVVKRLLADADVVTENYRKGT
                     LEKLGLGYDVLQALNPRLIYCAVSGYGRTGPYADKGGFDLIAQGFAGIMSITGEPGGP
                     PAKSGTSIADINAGILAALGIVSALVSRATTGRGQMVETSLMEAAIQQTYWQSAIFFA
                     TGVNPGPTGSAHILTAPYQAFPTKDGWINVGGANQSNWERIVKVIGRPELAEDARFRT
                     NGDRMKNLAALTPLIAERLLSRPSAEWILEFEAVGVPAGPVNKIGEMLGDPQVAARAM
                     VLEVDHPRAGRVKTLGTPIKFSATPGEVTRAAPVLGEHTREVLEGLGYTSAEIDHLRN
                     EGAVLVAE"
BASE COUNT          338 a          649 c          672 g          279 t
ORIGIN      
        1 gaggcgcgcg actacggcaa cgacatgcgc gccaccatcg aggccatcga ccgctgccgc
       61 cacccggtgg tcgcgcagat ccacggcatc tgcgtgggcg gcggcctcga gatcgccggg
      121 ctcgccgaca tccgcatctg cggcaaatcg agccgcttcg gcgtgccgat caacaagctg
      181 gggctggtga tggcgtatgc cgagatcgga tcgctgatcg cgctcgcggg agaagcgacc
      241 gcgctcgaga tcctcctcga ggggcgcgtg ttcgatgcga aggaagcgaa ggagaagggc
      301 ctggtcacgc gcgtggtgcc ggacgccgag gtcgaaaccg aagcgcgcgc caccgcgcag
      361 cgcatcgccg acggcgcgcc gctggtcgcc cgctggcaca agaagttcgc gcgccgcctg
      421 cgcgatcccc gcccgctcac ccccgaggaa tacgacgagg gtttcgcctg cttcgggacc
      481 gaggacttcc gcatcggcta tcgggcgttt ctcgccaagg ccaaacccgc tttcgaaggc
      541 aaatgaacac cggggcgttg aagggaatca aggtcatcga gctcgcgcag atcatggcgg
      601 ggccgacgtg cgggatgctg ctcgccgaca tgggcgccga cgtgatcaag gtcgagaagc
      661 ttccgggcgg cgacgacacg cgcagctaca cggagccctc gatccggggc gagtcggcgg
      721 cgttcatgat cctgaaccgc aacaagcgcg ggatcgcggt gaacctgaag aagccgggcg
      781 gattggaagt cgtgaagcgc ctcctcgccg atgcggacgt cgtaaccgag aattaccgca
      841 agggcacgct cgagaaactc ggcctcggat atgacgtgct gcaggcgctg aatcctcgct
      901 tgatctactg cgcggtttcg gggtacggcc gcacgggccc ctacgcggac aagggcggct
      961 tcgacctcat cgcgcagggc ttcgcgggaa tcatgagcat cacgggcgag cccggcggcc
     1021 ctcccgcgaa gagcgggacg tcgatcgcgg acatcaacgc gggaatcctc gcggcgctcg
     1081 gcatcgtgtc ggcgctcgtg tcgcgcgcga cgaccggccg cgggcagatg gtggagacat
     1141 cgctcatgga agcggcgatc cagcagacct actggcagtc ggcgatcttc ttcgccacgg
     1201 gcgtgaatcc cggccccacc ggctccgcgc acattctcac cgcgccctac caggcgtttc
     1261 ccacgaagga cgggtggatc aacgtcggcg gcgcgaacca gtcgaactgg gagcgcatcg
     1321 tcaaggtgat cgggcgcccg gagctcgcgg aagatgcgcg cttcaggacc aacggcgatc
     1381 gcatgaagaa cctcgcggcg ctgacgccgc tcatcgcgga gcgcctgctc tcgcggccct
     1441 ccgcggaatg gatcctcgag ttcgaagcgg tgggcgtgcc ggccggcccg gtcaacaaga
     1501 tcggcgagat gctgggggat ccgcaggtcg cggcacgcgc gatggttctc gaggtcgatc
     1561 acccgcgcgc gggacgggtg aagaccctcg gcacgccgat caagttctcg gctacgcccg
     1621 gcgaggtcac ccgcgccgcg cccgtcctcg gcgagcacac tcgcgaggtc ctcgaggggc
     1681 tcgggtacac atcggccgag atcgatcatt tgcgcaacga aggcgcggta ctcgtcgccg
     1741 agtagaagcc actcgaacca gtttctactt gaattccacc gcggtagtgt tcgcgccgca
     1801 gaggagcacg cccaccgtgc tgccgcgctc gggctgccat gcgccgctga taagcgcggc
     1861 gagcgccgcg gccccgccgg gctcggccac gatgcgaagc gtcttccaca acgcgcgctg
     1921 ggcttcgcgg atcgcgtc
//
LOCUS       DASVII010000236         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_406904,
            whole genome shotgun sequence.
ACCESSION   DASVII010000236 DASVII010000000
VERSION     DASVII010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406904"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..779
                     /gene="uppS"
                     /locus_tag="VF309_03035"
     CDS             12..779
                     /gene="uppS"
                     /locus_tag="VF309_03035"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX7465589.1"
                     /translation="MSDFTSSTKVVPETGIVPRHVAIIMDGNGRWAKKRFLPRVAGHR
                     RGVEAVRETVKACAQCGVEYLTLFAFSSENWRRPPDEVSFLMQLFLRALEQEVGKLHK
                     NGIRFKVVGDLAPFDARVLEHIREGEALTRDNTKLTLTIAANYGGRWDILQAAERCRA
                     EDAAAPVTEERLAGFLSMAYAPEPDLFIRTGGEQRVSNFLLWQLAYTELHFTDALWPD
                     FGAQALEEAFDSYRSRERRFGRTSEQLKEGEPVKEAG"
     gene            787..1632
                     /locus_tag="VF309_03040"
     CDS             787..1632
                     /locus_tag="VF309_03040"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7465590.1"
                     /translation="MLRTRVITAVAILVVLIGMLFFASAIAWSLFTLAIALVACWEWS
                     RLSGLGARAQPAFLFASGAVGASFWLLYARDAQGAFTSAASAAFILSTAFWVIAAPAW
                     LATKARPSAGLCAAAGWLVVWPTWFAFVVLRDASPWLLLAIAALVWVADIAAYFAGRR
                     FGKGKLAPAISPGKTWEGVYGALAGVAVYGLVLSWISHGHDTPVSAIFADALGLPAIA
                     CMLVLVALSVVGDLLESWMKRGAGMKDSSALLPGHGGVLDRIDALTSTLPVAALALSL
                     RAGIP"
     gene            1629..2840
                     /gene="ispC"
                     /locus_tag="VF309_03045"
     CDS             1629..2840
                     /gene="ispC"
                     /locus_tag="VF309_03045"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7465591.1"
                     /translation="MKRLAILGSTGSVGRSTLDVAARHPDRFAVTALAAGRNDALLLE
                     QCIAHRPAHAVLADADAAERLRKAIAARGLATQVHCGRDALAAIVALEEVDTVMAAIV
                     GAAGLPATLAAARAGKQVLLANKEALVMSGALFMKAVAEGNATLLPIDSEHSAIFQSL
                     PRGFVCGRAGELERAGVRRILLTASGGPFRATPADRLSRVTPQQAVAHPNWVMGPKIS
                     VDSATMMNKGLEVIEAHWLFGAPLEAIQVVVHPQSVIHSMVEYADGSVVAQLGAPDMR
                     TPIACALAFPERIESGAAPLDFTTLGSLDFERLDAAKFPCVALAREAMRQGGTAPAAL
                     NAANEIAVEAFLGGRLSFTGIAEVIENVLGEVHVEPLESLEQVYAADIEARGLASRFI
                     ARRVPQSSLSV"
     gene            2837..>3018
                     /locus_tag="VF309_03050"
     CDS             2837..>3018
                     /locus_tag="VF309_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7465592.1"
                     /translation="MNWLTIPSFIVTVGVLVVIHEFGHYLAARLMDVKILRFSVGFGK
                     PLLASRRGADRTEWVLS"
BASE COUNT          399 a         1078 c         1041 g          500 t
ORIGIN      
        1 gcggtttagc gatgagcgac ttcaccagct cgaccaaagt cgtccccgaa acgggcatcg
       61 ttccgcgcca cgtcgcgatc atcatggacg gcaacgggcg ctgggcgaag aagcgcttcc
      121 tgccgcgcgt ggcgggacat cgccgcggcg tggaggcggt gcgcgagacg gtgaaggcgt
      181 gcgcgcaatg cggcgtcgag tacctgacgc tcttcgcgtt ctcgagcgag aactggcggc
      241 gcccgcccga cgaagtctcg ttcctgatgc agctcttcct gcgggcgctc gagcaggagg
      301 tcggcaagct gcacaagaac ggcatccgct tcaaggtcgt gggggacctc gcgcccttcg
      361 acgcgcgcgt gctcgagcac atccgcgagg gcgaggcgct cacgcgcgac aacaccaagc
      421 tcaccctcac catcgccgcc aactacggcg ggcgctggga catcctgcag gccgcggagc
      481 gctgccgcgc ggaagacgcc gcggcgccgg tcaccgagga gcgcctcgcg ggcttcctgt
      541 cgatggccta cgcgcccgag ccggacctct tcatccgcac cggcggcgag cagcgggtct
      601 ccaatttcct gctgtggcag ctcgcctaca ccgagctcca tttcaccgac gccctctggc
      661 cggacttcgg ggcgcaagcg ctcgaagagg ccttcgactc ctaccgcagc cgcgagcgcc
      721 gcttcggccg cacgagcgag cagctgaaag agggcgagcc ggtgaaagag gccggctagc
      781 ccgcccttgc tccgcacccg cgtcatcacc gcggtcgcga tcctggtggt tctcatcggg
      841 atgctcttct tcgcgtccgc gatcgcgtgg tcgctcttca cgctcgccat cgcgctggtc
      901 gcgtgctggg agtggtcgcg cctgagcggc ctcggggcgc gcgcccagcc ggcgttcctc
      961 ttcgcgagcg gcgccgtggg cgcatcgttc tggctcctct acgcgcgcga tgcgcaaggc
     1021 gcgttcacct ccgccgcctc ggccgcgttc atcctatcca ccgcgttctg ggtcatcgcc
     1081 gcgcccgcgt ggctcgcgac gaaggcccgg ccttccgcgg ggctgtgcgc ggccgcgggc
     1141 tggctcgtgg tctggccgac gtggttcgcg ttcgtcgtgc tgcgcgatgc gagcccgtgg
     1201 ctcctcctcg cgatcgcggc cctcgtgtgg gtcgcggaca tcgccgcgta tttcgcggga
     1261 cggcgcttcg gcaaggggaa gctcgccccg gcgatcagcc cgggcaagac gtgggagggc
     1321 gtttacggag cgcttgcggg cgtggccgtg tacggcttgg tgttgtcctg gatttcccat
     1381 gggcacgaca cgccggtctc cgcgatcttc gcggacgcgc tcgggctccc ggcgatcgcg
     1441 tgcatgctgg tcctcgtcgc gttgagcgtc gtcggcgatc tcctggagtc gtggatgaag
     1501 cgcggcgcgg ggatgaagga ttcgagcgcg ctcctgccgg ggcacggcgg ggtcctcgac
     1561 cgcatcgacg ccctcacgtc caccttgccg gtcgcggcgc tcgcgctgtc gctgcgcgcg
     1621 gggatcccat gaagcgcctc gcgatcctgg gctccacggg ctcggtgggc aggagcacgc
     1681 tcgatgtcgc ggcgcgccat ccggatcgtt tcgcggtgac ggcgctcgcc gcgggccgca
     1741 acgacgcgct cctcctcgag caatgcatcg cgcatcggcc ggcgcacgcg gtgctcgccg
     1801 acgccgacgc ggccgagcgc ctgcgaaaag ccatcgcggc gcgcgggctt gcgacgcagg
     1861 tgcattgcgg ccgcgacgcg ctcgccgcga tcgtcgcgct cgaagaggtc gacacggtga
     1921 tggcggccat cgtcggcgcc gcgggacttc ccgcgacgct cgccgccgcg cgcgccggca
     1981 agcaggtgct cctcgccaac aaggaggcgc tcgtcatgtc gggcgcgctc ttcatgaagg
     2041 cggtcgccga aggcaacgcg acgctccttc ccatcgacag cgagcattcc gcgatcttcc
     2101 agtcgctgcc gcgcgggttc gtgtgtggcc gcgcgggcga gctcgagcgc gcgggcgtgc
     2161 gccgcattct gctcaccgcc tcgggcggcc cgtttcgcgc gacgccggcg gaccggctat
     2221 cgcgcgtcac gccgcagcag gcggtcgcgc atcccaactg ggtgatgggc ccgaagatct
     2281 ccgtcgattc cgcgaccatg atgaacaagg gcctcgaagt gatcgaggcc cattggctct
     2341 tcggcgcgcc gctggaggcg atccaggtgg tcgtgcatcc gcagagcgtg atccattcca
     2401 tggtcgagta cgccgacggc tccgtcgtgg cccagctcgg cgctcccgac atgcgcacgc
     2461 ccatcgcgtg cgcgctcgcg tttccggagc gcatcgagtc gggcgccgcg cccctcgact
     2521 tcacgactct cggcagcctc gatttcgagc gcctcgacgc cgcgaaattc ccgtgcgtcg
     2581 cgctcgcccg ggaggcgatg cgccagggcg gcaccgcccc cgcggcgctg aacgccgcca
     2641 acgagatcgc ggtcgaagcg tttctcggcg ggaggctttc cttcaccgga atcgccgaag
     2701 tcatcgaaaa tgtgctggga gaagtccacg tggagccgct ggagagcctc gaacaggtct
     2761 atgctgcgga tatcgaagca cgcggcctcg cctcccgatt catcgcgcgc cgcgtcccgc
     2821 aatcctccct cagcgtatga actggctcac catcccctcg tttatcgtga cggtcggcgt
     2881 tctcgtggtg atccacgagt tcggccacta cctcgccgcg cgcctcatgg acgtgaagat
     2941 cctgcggttc tccgtgggct tcgggaaacc gcttctcgcg agccgccgcg gggccgaccg
     3001 caccgagtgg gtcctttc
//
LOCUS       DASVII010000237         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_550906,
            whole genome shotgun sequence.
ACCESSION   DASVII010000237 DASVII010000000
VERSION     DASVII010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550906"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /gene="aceE"
                     /locus_tag="VF309_03055"
     CDS             <1..1447
                     /gene="aceE"
                     /locus_tag="VF309_03055"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX7465593.1"
                     /translation="AAQGRMTGHQHKKLDTDQLIAFRDRFALPFADAQVEACEFVRPP
                     REAPEMAYLHARREKLGGYLPMRSTKAESLATPDAAAFAKFALEANGKEMSSTVAYVR
                     MVGALLKDPVLGKRIVPIIADEARTFGMADLFRQIGIYSPIGQLYQPEDQHQLSYYKE
                     STDGQILEEGINEAGALASWVAAATSYSAHGYGMLPMYIYYSMFGFQRVGDEIWAAAD
                     SRSRGFLFGATAGRTTLSGEGLQHQDGTSHLIASTVPNCRAYDPCFAYELAVILEDGM
                     RRMLGEQEDVFYYVTLMNESYAMPSMPGDAREGILRGMYKARGTRGEKARVRLLGAGT
                     ILNEALAAAEMLDKEWGVPAEVFSVTSFTELRRDGMEVSRHHRAGGKAAKPPWVERQL
                     PKTGVPVVAASDYVSAVADLIRPWIADPYTALGTDGFGRSDTRANLRKFFEVDRHAIA
                     VAALHSLGDKRVAEAMGRYGIDPAAAPPWAR"
     gene            complement(1459..>1588)
                     /locus_tag="VF309_03060"
     CDS             complement(1459..>1588)
                     /locus_tag="VF309_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX7465594.1"
                     /translation="FLDIASDAAWGRPVDVQVMPDGALLVSDDQAGAIYRIAYRAP"
BASE COUNT          269 a          529 c          556 g          234 t
ORIGIN      
        1 cgcggcgcag gggcgcatga ccgggcacca gcacaagaag ctcgacaccg accagttgat
       61 cgctttccgc gaccgcttcg cgctgccatt cgccgatgcg caggtcgaag cgtgcgagtt
      121 cgtccgcccg ccgcgcgagg cgcccgagat ggcgtacctg catgcgcggc gggagaaatt
      181 gggcggctac ctgccgatgc gctcgaccaa ggcggaatcg ctcgccacgc ccgacgcggc
      241 cgcattcgca aagttcgcgc tcgaggcgaa cggcaaggag atgtcctcga cggtggcgta
      301 cgtgcgcatg gtgggcgcgc tcctcaagga ccccgtgctc ggcaagcgca ttgtccccat
      361 catcgccgac gaggcgcgca cgttcggcat ggcagacctc ttccgccaga tcggcatcta
      421 ctcgccgatc ggccagctct accagcccga ggaccagcac cagctctcgt actacaagga
      481 gtccaccgac gggcagatcc tcgaggaagg catcaacgag gcgggcgcgc tcgcttcgtg
      541 ggtcgccgcg gcgaccagct actcggcgca cggctacggg atgctgccga tgtacatcta
      601 ctattcgatg ttcggcttcc agcgcgtggg cgacgagatc tgggccgcgg ccgactcgcg
      661 ttcccgcggc ttcctcttcg gcgcgaccgc cgggcgcacc acgctttccg gcgagggatt
      721 gcagcaccag gacggcacga gccacctcat cgcgtcgacc gtgccgaact gccgtgcgta
      781 cgatccgtgc ttcgcctacg agctcgcggt gatcctcgag gacggcatgc gccgcatgct
      841 cggcgagcag gaagacgtct tctactacgt gacgctcatg aacgagagct acgcgatgcc
      901 gtcgatgccc ggggacgcgc gcgagggcat cctgcgagga atgtacaagg cgcgaggcac
      961 gaggggcgag aaggcgcgag tgcggttgct gggcgcaggg acgatcctca acgaagcgct
     1021 tgccgcggcc gagatgctgg acaaggaatg gggagtaccg gctgaggtgt tttccgtgac
     1081 cagcttcacg gaattgcggc gcgatggcat ggaggtttca cgccaccatc gcgcgggcgg
     1141 aaaagcggcc aagcccccgt gggtggaacg gcagctgccg aagaccggcg tcccggtggt
     1201 ggcggcgagc gactacgtga gcgccgtcgc cgacctcatc cgcccttgga tcgcggaccc
     1261 gtacacggcc ctgggaaccg acggcttcgg gcgaagcgac acgcgcgcga acctgcgcaa
     1321 gttcttcgag gtggatcgcc acgcgatcgc cgtggcggca ttgcattcgt tgggagacaa
     1381 gcgcgtcgcg gaagcgatgg gccggtacgg catcgacccg gccgccgcgc cgccctgggc
     1441 gcgctgacgc gccggtgcct agggcgcgcg ataggcgatg cggtagatcg cacccgcctg
     1501 gtcgtcggag acgaggagcg ctccgtcggg catgacctgc acgtccacgg ggcgtcccca
     1561 cgcggcgtcg ctcgcgatgt ccaggaac
//
LOCUS       DASVII010000238         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_299024,
            whole genome shotgun sequence.
ACCESSION   DASVII010000238 DASVII010000000
VERSION     DASVII010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299024"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VF309_03065"
     CDS             <1..1345
                     /locus_tag="VF309_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX7465595.1"
                     /translation="LWKWSPWVVGLVIGSMFVVDFMFLASNAAKFLSGGWFPVLMGAV
                     FLALTGGEALYADMGHFGKRPIRIAWLSLVFPALLLNYFGQAAFVLANPAGIKNPFYL
                     MVPEWGLYPMVVLATAATVIASQAAISGAFSMTRQAIQLGYIPRLEILHTSERSIGQI
                     YVPFVNWSLFVAVVLLVLGFQSSENLANAYGIAVAATMVIECILAMVVARLLWKWSPW
                     VVGLVIGSMFVVDFMFLASNAAKFLSGGWFPVLIGTAIFSLLMTWKRGRTLMFRRLSE
                     QGIPLKPFLEGLEAHPPVKVQGTAIFMTANVDTVPHALLHNLKHNKILHEQTLFLTVI
                     SHDVPSVPKEDRVQLERLVNGFFKLEAWYGFKEQPDIDEILNACRVRYGLAFDVMDTS
                     FFLSRETVIPTSDVPGMALWRDHVFAWMTRNATRATDFFNIPANRVVELGTHIEI"
     gene            1398..>1684
                     /locus_tag="VF309_03070"
     CDS             1398..>1684
                     /locus_tag="VF309_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465596.1"
                     /translation="MSSQIDSARSHPAVAAAVVVGGIAFTAGALLVIAYVLDWLPSKP
                     QLPNSPTSMANPGQQVAGSAPDVALLPGETLVAPADAPPTPEPAKPAAPSAA"
BASE COUNT          242 a          575 c          550 g          317 t
ORIGIN      
        1 gctgtggaag tggtcgccgt gggtggtcgg cctcgtgatc ggcagcatgt tcgtggtcga
       61 cttcatgttc ctcgcctcga acgcggcgaa gttcctctcc ggcggctggt tcccggtgct
      121 catgggcgcc gtgttcctcg cgctcacggg aggcgaggcg ctgtacgcgg acatgggcca
      181 cttcggcaag cggccgattc gcatcgcctg gctgagcctg gtgtttccgg cgctcctcct
      241 gaattacttc ggccaggcgg cgttcgtgct cgccaatccc gcgggcatca agaatccgtt
      301 ctacctcatg gtgccggaat ggggactcta tccgatggtg gtcctcgcca cggcggccac
      361 ggtgatcgcg tcccaggccg ccatctcggg ggcgttctcg atgacgcgcc aggcgatcca
      421 gctgggatac atcccgcgcc tcgagatcct gcacacgtcc gagcgctcca tcgggcagat
      481 ctacgtcccc ttcgtgaact ggtcgctctt cgtggcggtg gtcctgctgg tgctcggctt
      541 ccagagctcc gaaaacctcg ccaatgccta cggcatcgcg gtggcggcga ccatggtgat
      601 cgagtgcatc ctcgccatgg tggtggcgcg cttgctgtgg aaatggtcgc cgtgggtggt
      661 cggcctcgtg atcggcagca tgttcgtggt cgacttcatg ttcctcgcct cgaacgcggc
      721 caagttcctc tccggcggct ggttcccggt gctcatcggc accgcgatct tcagcctcct
      781 catgacgtgg aagcgcggcc gcacgctcat gttccggcgc ctctcggagc agggaatccc
      841 gctgaagccg ttcctcgagg gcctcgaggc ccatccgccc gtgaaggtgc aggggacggc
      901 gatcttcatg acggcgaatg tcgacaccgt tccccatgcc ctgctgcaca acctcaagca
      961 caacaagatc ctccacgagc agacgctctt cctcaccgtc atttcccacg acgtgccgtc
     1021 ggtcccgaag gaggaccgcg tgcagctgga gcgcctcgtg aacggcttct tcaagctcga
     1081 ggcgtggtac gggttcaagg agcagcccga catcgacgag atcctcaacg cgtgccgcgt
     1141 gcgctatggg ctcgcgttcg acgtgatgga cacgtcgttc ttcctttcgc gcgagacggt
     1201 gattccgacg agcgacgtgc cgggcatggc gctctggcgc gaccacgtgt tcgcgtggat
     1261 gacgcgcaac gcgacgcgcg cgacggactt cttcaacatt cccgcgaacc gcgtggtgga
     1321 actgggcacg cacatcgaaa tctaacggcg gtcccctggg catccaggcc ccggggcgcg
     1381 catggagtcg tcccggcatg agctcgcaga tcgattcggc ccgctcccat cccgccgtcg
     1441 ccgccgccgt ggtggtcggc ggcatcgcgt tcacggcggg cgcgctcctc gtcatcgcct
     1501 acgtgctcga ctggcttccc tcgaagccgc aactgccgaa ctcgccgacc agcatggcga
     1561 atccggggca gcaggttgcg ggaagcgcgc cggatgtcgc gctgctgccg ggcgaaacgc
     1621 tggtggcgcc cgcggacgcg ccgccgacgc ccgagcccgc gaagcccgcg gcgccgtcgg
     1681 ctgc
//
LOCUS       DASVII010000239         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_299074,
            whole genome shotgun sequence.
ACCESSION   DASVII010000239 DASVII010000000
VERSION     DASVII010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299074"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF309_03075"
     CDS             complement(<1..357)
                     /locus_tag="VF309_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7465597.1"
                     /translation="MRKLLWALPLLAALTLSGCGYNDFQTRDEQVKAAWSEVLNQYQR
                     RADLIPNLVATVQGFAAQEKDVLIGVTQARARATGVTVSPETLNDPAAMQKFQQAQGE
                     LSGALGRLLAVAENYPQ"
     gene            complement(425..1036)
                     /locus_tag="VF309_03080"
     CDS             complement(425..1036)
                     /locus_tag="VF309_03080"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7465598.1"
                     /translation="MYGGRAPGDEVARPVAEMDWDALREAVRACTACGLCRQRKQAVF
                     GVGAESATWLFIGEGPGADEDELGEPFVGQAGKLLDSMLAAIGIKRGREAYIANVVKC
                     RPPGNRTPAAEEAGACAPFLDRQIELVKPKIIVALGKTAAARLLDTEASLGSLRGKAH
                     GYKGTPLVVTYHPAYLLRSLPEKAKAWEDLVFARSTLAGATAA"
     gene            complement(1084..>1516)
                     /gene="rimI"
                     /locus_tag="VF309_03085"
     CDS             complement(1084..>1516)
                     /gene="rimI"
                     /locus_tag="VF309_03085"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX7465599.1"
                     /translation="YRRMRAADLAEVAQLEKSLYGFPWSLGNFRDSVNAGYDCWTVTH
                     GERVIGYAILMIALDEAHLLNIAIAGEWQNQGIGGDFLEHMIRVAKAAACQIVYLEVR
                     PSNLAARHLYRTMGFQQIAIRPEYYPAVAGREDALFLGLTL"
BASE COUNT          224 a          518 c          506 g          268 t
ORIGIN      
        1 ctgcggatag ttttccgcga ccgcgagaag gcgcccgagc gccccggaga gctcgccctg
       61 tgcctgctgg aatttctgca tcgccgcggg atcgttcaag gtctcgggcg agacggtcac
      121 cccggttgcg cgcgcgcgcg cctgcgtaac gccgatgagt acgtcctttt cctgcgcggc
      181 aaagccttgc acggtggcga cgagattggg gatgagatcc gcgcggcgct gatactggtt
      241 cagcacctcg gaccaggcgg ccttcacctg ctcgtcgcgc gtctggaagt cgttgtagcc
      301 gcaacctgaa agcgtcagcg ccgcgagaag cggcagggcc cacagaagct tgcgcattgt
      361 tctatctccc gggtacggcc cgcgatcggg ccagcgctga ggatgggggc gggatcgggt
      421 gttttcaagc cgccgtcgcg ccggcgagcg tgcttctcgc gaatacgagg tcttcccacg
      481 ccttggcttt ctccggaagg ctgcgcagga ggtaggcggg atggtaggtc acgacgagcg
      541 gcgtgccctt gtagccgtgc gccttgccgc gcaggctccc gaggctcgcc tccgtgtcca
      601 gcaggcgggc cgccgcggtc ttgccgagcg cgacgatgat cttcggtttc acgagctcga
      661 tctgccggtc gaggaagggc gcgcatgcgc cggcctcctc cgccgccggg gtgcgattgc
      721 ccggcgggcg gcacttcacg acgttcgcga tgtaagcctc gcggccgcgc ttgatgccga
      781 tcgcggcgag catgctgtcg agcagcttcc ccgcctggcc cacgaacggc tcgcccagtt
      841 cgtcctcgtc ggcgccgggg ccttctccaa tgaagagcca cgttgccgat tcggcgccca
      901 cgccgaacac cgcttgcttg cgctgcctgc atagtccgca agcggtgcac gcccgcaccg
      961 cttccctcag ggcgtcccag tccatttcgg ccaccggcct cgcgacctcg tcccccgggg
     1021 ctcgcccccc gtacaggcga acgacgttcg cgagctcgag ctccttcagg attcgctctt
     1081 gcgtcacaac gtcagcccga ggaagagcgc gtcttcgcgg cccgccacgg ccgggtagta
     1141 ctcgggccgg atcgcgatct gctggaagcc catcgtgcga tagaggtgcc gcgccgccag
     1201 gttcgagggg cgcacctcga gatagacgat ctggcaggcc gcggctttcg ccacgcggat
     1261 catgtgctcc aggaaatcgc cgccgatgcc ctggttctgc cattcgccgg cgatcgcgat
     1321 gttgagcaga tgcgcctcgt cgagcgcgat catgaggatc gcgtagccga tcaccctctc
     1381 gccatgagtc acggtccagc agtcatatcc cgcgttcacc gagtcgcgga aattgccgag
     1441 gctccaggga aagccgtaaa gcgacttctc gagctgcgcg acctcggcga ggtcggcggc
     1501 gcgcatgcgt cgatag
//
LOCUS       DASVII010000240         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_694740,
            whole genome shotgun sequence.
ACCESSION   DASVII010000240 DASVII010000000
VERSION     DASVII010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694740"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VF309_03090"
     CDS             complement(<1..246)
                     /locus_tag="VF309_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465600.1"
                     /translation="MSPITSAIEGPLHPGRWWHRLDDARVQCDLCPRDCRLHEGQRGA
                     CFVRARVGDAMVLTTYGRSSGFCVDPIEKKPLNHFLPG"
     gene            complement(243..788)
                     /gene="amrA"
                     /locus_tag="VF309_03095"
     CDS             complement(243..788)
                     /gene="amrA"
                     /locus_tag="VF309_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX7465601.1"
                     /translation="MPEVIERGTVLLAIAREAIADPGEPCGEFTRGHEWLRSPAASFV
                     TLRAEGELRGCIGSIDARRPLGDDVARNAHAAAYRDPRFPPLQPGERASVAIEVSVLT
                     PRVPLAAASEGEALAILRPGEDGIYLQYLDTSATFLPQVWENLPDPMDFLAELRRKAG
                     LPARFWHPDIRLSRYTVEKYR"
     gene            complement(781..1563)
                     /gene="amrB"
                     /locus_tag="VF309_03100"
     CDS             complement(781..1563)
                     /gene="amrB"
                     /locus_tag="VF309_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX7465602.1"
                     /translation="MAGSFYPGDPSALAAEVAAYLAEASTGGTRSQAPKAIIAPHAGY
                     IYSGAVAASIYARLAPLRGIVRRVVLAGPAHRVYVRGAAIPGVDAFASPLGAVRLDLP
                     ALARLRELDFVEVSDAAHALEHSLEVHLPFLQSVLGEVALVPIVVGDATPREMARLFG
                     LLWGGDETLIVVSSDLSHYLPYDAARGRDRDTARAIVGLEASLVPEEACGAAPINGLL
                     EAARERGLRAEMVDLRNSGDTAGGLDRVVGYGAFAFTEARHA"
     gene            complement(1649..>2076)
                     /locus_tag="VF309_03105"
     CDS             complement(1649..>2076)
                     /locus_tag="VF309_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465603.1"
                     /translation="LLLNNGVIEQQGTPQQMYAEPQTLFTADFMGSNNRLAGQVSELQ
                     GDAALLTGEGWQLRGRAKPSAKMGAAGTGLIRLERVRVADGPGDNRVQPELKTSMFLG
                     DKWEHVFNLGATMLRAHGPRALSPGKHWVELPREDFWIF"
BASE COUNT          332 a          729 c          731 g          284 t
ORIGIN      
        1 ccccggcagg aaatgattga gcggtttctt ctcgatggga tcgacgcaga atcccgagga
       61 ccgtccgtac gtcgtgagca ccatcgcatc gcccactcgc gcgcgcacga agcacgcgcc
      121 gcgctggcct tcgtgcaggc ggcaatcgcg cgggcagagg tcgcactgga cgcgcgcatc
      181 gtcgagccgg tgccaccacc gccccggatg cagcgggcct tcgatcgcgc tcgtgatcgg
      241 gctcaccggt acttctccac cgtgtagcgc gacagccgga tgtcggggtg ccagaagcgc
      301 gccggcagtc ccgccttgcg ccgcaattcg gcgaggaagt ccatcggatc gggaaggttc
      361 tcccagacct gcggaaggaa cgtcgcgctc gtgtcgaggt actgcaggta gatgccatcc
      421 tcgccgggac gcaggatcgc aagcgcctcg ccctcgctcg ccgccgccag cggcacgcgc
      481 ggcgtgagca ccgagacctc gatcgcgacc gaggcgcgtt cgcccggctg caacggtgga
      541 aaccgcggat cgcgatacgc ggccgcgtgc gcgttgcgcg cgacgtcgtc gcccagcggg
      601 cggcgcgcgt cgatcgatcc gatgcatccg cgcagctcgc cctcggcgcg cagcgtgacg
      661 aagctcgccg ccggcgagcg cagccactcg tgtccgcgcg tgaactcgcc gcacggctcg
      721 ccggggtcgg cgatcgcctc gcgggcgatc gcgagcagca ccgtgccgcg ctcgatcacc
      781 tcaggcatgc cgcgcctcgg tgaaagcgaa cgcgccgtat ccgacgacgc ggtcgagccc
      841 gcccgcggtg tctccggaat tgcgcaggtc caccatctcg gcacgaagcc cacgctcgcg
      901 cgccgcctcg agaagcccgt tgatcggcgc cgcgccgcac gcctcctcgg ggaccaggct
      961 cgcttcgagg ccgacgatgg cgcgcgcggt gtcgcgatcg cggccgcgcg ccgcatcata
     1021 aggcaggtag tgggaaagat cggagctcac cacgatgagc gtctcgtcgc ctccccacag
     1081 caggccgaag agccgcgcca tttcgcgcgg tgtcgcatcg cccaccacga tcggaacgag
     1141 cgcgacctcg ccgagcaccg actggagaaa gggcagatgc acttcgaggg aatgctcgag
     1201 cgcgtgcgcc gcgtcgctca cctcgacgaa atcgagctca cgcaaccgtg cgagcgccgg
     1261 gagatcgagg cgcacggcac cgagcggcga agcgaacgcg tcgacgccag gaatggcggc
     1321 gccgcgcacg tagacgcgat gcgcgggacc cgcgagcacc acacgtcgaa cgattccgcg
     1381 aaggggcgcc aggcgcgcgt agatcgatgc ggcgaccgcg cccgagtaga tgtagcccgc
     1441 gtggggcgcg atgatcgcct tcggcgcctg tgagcgcgtg ccgccggttg aggcttcggc
     1501 aagataggcc gccacctcgg cggcgagggc cgaaggatca cccggataga aggaccctgc
     1561 cacggcggcg ggtcgaatgg cggacatgac tcacctgcgt gcgacgccga ccttccagcc
     1621 tagcacttcc cgacgccgag ttgcggcgct agaagatcca gaagtcctcg cgggggagct
     1681 cgacccagtg cttgcccggg gaaagggcgc gtgggccgtg ggcgcgcagc atcgtggcgc
     1741 ccaggttgaa gacgtgctcc cacttgtcgc cgaggaacat cgaagtctta agctccggct
     1801 ggacgcggtt gtcgccgggg ccgtcggcta cgcgcacgcg ctcgaggcgg atgagtcccg
     1861 tacccgccgc gcccatcttc gcgctgggct tggcgcgccc gcgaagctgc cagccctcgc
     1921 ccgtgaggag cgccgcatcg ccctgcagct ccgagacctg gcccgcgagg cggttgttgc
     1981 tgcccatgaa gtcggcggtg aagagcgtct gcggctcggc gtacatctgc tggggcgtgc
     2041 cctgctgctc gatgaccccg ttgttgagga ggagga
//
LOCUS       DASVII010000241         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_442993,
            whole genome shotgun sequence.
ACCESSION   DASVII010000241 DASVII010000000
VERSION     DASVII010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442993"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VF309_03110"
     CDS             complement(<1..845)
                     /locus_tag="VF309_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7465604.1"
                     /translation="MAKMSGAQALADMLQGYGVTHVFMVPAVLRRTFAEMERRTQIQR
                     IHCHGEKAAAYMADGYARASGKPGVCMAQVVGALNLAAGLRDAWLAHSPVIAFTGGRD
                     AKTKFRKVYQEVDDVPSFEPVTKWNATVDAVERIPDMVRQAFRAAVSGAPGPVHLQFR
                     GNEGQIDQEEFDVEPFWESQFSQVPPFRPEPDAASVRAVLALLQQAQKPIFVAGGGVR
                     ASGAARELVALAEKLQIPVATSLNAKDAIPGTHPLSVGVVGSYSRESANRAVAAADLV
                     CFIGT"
     gene            complement(854..1825)
                     /locus_tag="VF309_03115"
     CDS             complement(854..1825)
                     /locus_tag="VF309_03115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7465605.1"
                     /translation="MAAAKLTRKDLALAAEKYKSWGKWGPADEIGTLNNTTAEDIVAA
                     ARLVRKGKVISLALNFDQHGPQGAKSKYPSLGRINPLHTMLRTGTDAYSGVLDHRGIR
                     AADDMVTMPLQCGTQWDGLGHIFYENYMWNGYDCREVTSAGAQKCGIEKTKAKMVGRG
                     VFLDVPRALGKDVLEDGHGITCAELDATAKKQGVEVKKGDFVVVRTGQMETKLKAGSW
                     DGYPGGDAPGFAFETLEWVKRTELAALASDTWGCEVRPNETEPGINQPWHWITIPMMG
                     MTMGEIFYVKELAEDCAADGIYEFLFVAPAIPITGAVGSPTNPLAIK"
     gene            1963..2373
                     /locus_tag="VF309_03120"
     CDS             1963..2373
                     /locus_tag="VF309_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465606.1"
                     /translation="MNRKPSLKQVAGFCVLAVALHGAAIAAEARLTRLVCTVRAGSGD
                     EHQIDIRNSASQVLAKDTIINYGVKWARPTMPGQLSGCFAIDKDLAANVSVSQAIKLG
                     PGAVPQSCTAFVSSTIPLATHGKDGSTEVRCSDS"
BASE COUNT          371 a          874 c          789 g          430 t
ORIGIN      
        1 tcggtgccga tgaagcacac gagatcggcc gcggccaccg cgcgattggc gctttcgcgc
       61 gaatacgagc ccaccacgcc caccgagagc ggatgcgtgc cggggatcgc gtccttcgcg
      121 ttcaatgacg tcgccacggg aatctgcagc ttctcggcga gcgccaccag ctcgcgcgcc
      181 gcacccgagg cgcgcacgcc gccacccgcg acgaagatcg gcttctgtgc ctgctgcagc
      241 agcgcgagca ccgcgcgcac gctcgccgca tcaggctcgg ggcggaatgg cggcacttgc
      301 gagaactggc tctcccagaa gggctcgacg tcgaactcct cctggtcgat ctgcccctcg
      361 ttgccgcgga actgcaggtg gaccggcccc ggcgcgccgc tcaccgccgc gcgaaacgcc
      421 tggcgcacca tgtccggaat gcgctccacc gcgtcgacgg tcgcgttcca tttggtcacc
      481 ggctcgaagg acggcacgtc gtcgacttcc tggtagacct tgcggaactt ggtcttcgca
      541 tcgcgcccgc cggtgaaggc gatcaccggc gagtgcgcga gccacgcgtc gcgcaatccc
      601 gcggcgaggt tcagcgcgcc cacgacctgc gccatgcaca cgccgggctt ccccgaggcg
      661 cgcgcgtagc catcggccat gtacgccgcc gccttctctc cgtggcagtg gatgcgctgg
      721 atctgcgtgc ggcgctccat ctcggcgaac gtgcggcgca gcaccgcggg caccatgaac
      781 acgtgggtca cgccataacc ctgcagcatg tcggcgagcg cctgcgcgcc gctcatcttg
      841 gccatggcgt tccttacttg atcgcgaggg gattggtggg cgagccgacc gcgccggtga
      901 tcgggattgc gggcgcgacg aagagaaact cgtagatgcc gtcggcggcg caatcctcgg
      961 cgagctcctt cacgtagaag atctcgccca tggtcatgcc catcatcggg atcgtgatcc
     1021 agtgccacgg ctggttgatg ccgggctcgg tctcgttggg gcgcacttcg cagccccagg
     1081 tgtcgctcgc gagcgcggca agctcggtgc gcttgaccca ctcgagcgtc tcgaaggcga
     1141 agccgggcgc gtcgccgccg ggatagccgt cccagctgcc cgccttgagc ttcgtttcca
     1201 tctgcccggt gcgcaccacg acgaagtcgc ccttcttcac ttccacgccc tgcttcttcg
     1261 ccgtcgcgtc gagctcggcg caggtgatgc cgtggccgtc ctcgaggacg tccttgccga
     1321 gcgcgcgcgg cacgtcgagg aacacgccgc gccccaccat cttcgccttg gtcttctcga
     1381 tgccgcactt ctgcgcgccc gcggaagtca cctcgcggca gtcgtagccg ttccacatgt
     1441 agttctcgta gaagatgtgg cccaggccat cccactgcgt gccgcactgc agcggcatcg
     1501 tcaccatgtc gtcggcggcg cggatgccgc gatggtcgag cacgcccgag tacgcgtcgg
     1561 tgccggtgcg cagcatcgtg tgcagcgggt tgatgcgccc gagcgacgga tacttgctct
     1621 tcgcgccttg cgggccgtgc tggtcgaagt tcaacgcgag cgagatgacc ttgcccttgc
     1681 gcaccaggcg cgcggccgcg acgatgtcct cggccgtggt gttgttgagc gtgccgatct
     1741 cgtcggcggg accccacttg ccccagctct tgtatttctc ggccgcgagg gccaggtcct
     1801 tgcgcgtgag cttcgctgcc gccatcactt cctccttgtc gtttttctcg tcgccattct
     1861 atgcctaacg tcgccggact cgacctttcg ccggcgattg cggatatgat tccgcggaag
     1921 cgcgccagcc ccgcgccgtc attcatgaaa gaggaggcgc cgatgaacag gaagccatcg
     1981 ttgaagcagg tcgccggatt ctgcgtcctg gccgtcgcgc tccacggcgc ggcgatcgcc
     2041 gcggaagcaa ggttgacacg cctcgtctgc accgtgcgcg caggaagcgg cgacgagcac
     2101 cagatcgata tccgcaactc ggcgagccag gtccttgcga aggacaccat catcaactat
     2161 ggcgtgaaat gggcgaggcc gaccatgccg ggccaactct ccggctgctt cgcgatcgac
     2221 aaggatctcg cggcgaatgt ctccgtcagc caggcgatca agctcggtcc cggcgcggtg
     2281 ccgcaatcgt gcactgcctt cgtctcgagc acgatcccct tggccaccca cggcaaggac
     2341 gggagcaccg aagtccgctg ctcggacagc tagtcccggg cgccctcggc gggatggccg
     2401 ctcgcgcgcc agcggatcat tccgccggcg agattcgcca ccttctcgaa tcccaatcgc
     2461 tgca
//
LOCUS       DASVII010000242         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_658991,
            whole genome shotgun sequence.
ACCESSION   DASVII010000242 DASVII010000000
VERSION     DASVII010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658991"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF309_03125"
     CDS             <1..303
                     /locus_tag="VF309_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465607.1"
                     /translation="VFYGARGVRDVCGNAELAALPGFGTRITFTPVLSEPQAGPVETW
                     QGATGFVHEIARDFVGERWTEFEYYFAGPPPMAKAVQQMLIEKRVPYPQVHFDSFY"
     gene            325..1317
                     /locus_tag="VF309_03130"
     CDS             325..1317
                     /locus_tag="VF309_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465608.1"
                     /translation="MSKLRLSLGCWNYDRTRGLLEGRIQPDGIDLTYLNMPVEETFFR
                     MLRHREFDVAEMSLSSYTVSLFKEKKDFIAIPVFPSRFFRHSCIYVNANAGIREPKDL
                     IGKKVGNPEYQMTAPVWIRGILQDEYGVPIDSVTYCTGGEEEPGRSEKIKLDLPPRIK
                     VQPIGERDTLAAMLRDGGIDALYTARMPSTYRTGGGKVKRLFESYMEVEQAYYRKTRI
                     FPIMHTVAIRRSLYEKHPWVAMSLYKAFVQAQRETYDDLYVTAALKAMLPWLTKHVED
                     ARALMGDDFWPYGFAPNRETLATFLRYHHEQGLSKKLLAPEELFAPETMESFKI"
     gene            1317..>1817
                     /locus_tag="VF309_03135"
     CDS             1317..>1817
                     /locus_tag="VF309_03135"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX7465609.1"
                     /translation="MAEPLGTKKHTLGDHLMGEGGLHAHAEGEYDHDHDHDDLGEHDL
                     ENDALWQQDHITLVSVGIDIGSSGTQVIFSRIYMRRLAEALTSRYFVVKRETLYQSPV
                     ALTPYQSETRIDERAVGDIVDAAYTAAGIHPDQIDTGAVILTGEALRRENAKAIADVI
                     AEMGGEF"
BASE COUNT          346 a          600 c          585 g          286 t
ORIGIN      
        1 gtgttctacg gcgcgcgcgg cgttcgcgac gtgtgcggca acgcggagct cgcggcgctt
       61 cccgggttcg gcacgcgcat cactttcacg ccggtgttgt cggagcccca ggccggtccc
      121 gtggagacct ggcagggggc gaccgggttc gtgcacgaga tcgcgcgcga cttcgtcggc
      181 gagcgctgga ccgaattcga gtactacttc gccggcccgc cgccgatggc gaaagccgtg
      241 cagcagatgc tgatcgaaaa gcgcgtgcca tacccgcaag tccacttcga cagtttttac
      301 taggcacgag gctcgaggcg cgagatgtcc aaacttcggc tttcactggg atgctggaac
      361 tacgaccgga ctcgcgggtt gctcgaaggg cgcatccagc ccgacggcat cgacctcacg
      421 tacctgaaca tgccggtgga ggagaccttc ttccggatgc tgcgccaccg ggagttcgac
      481 gtcgccgaga tgtcgctgtc gtcgtacacg gtgtcgctct tcaaggagaa gaaggacttc
      541 atcgcgatcc ccgtattccc gtcgcgcttt ttccggcact cgtgcatcta cgtgaacgcc
      601 aatgcgggga tccgcgaacc gaaagacctc atcgggaaga aggtcggcaa ccccgagtac
      661 cagatgacgg cgcccgtctg gatccgcggc atcctgcagg acgagtacgg cgttcccatc
      721 gacagcgtca cgtattgcac cggaggcgag gaggagcccg gccgttccga gaagatcaag
      781 ctcgatctcc cgccgcgcat caaggtgcag ccgatcggcg agcgcgacac gctcgcggca
      841 atgcttcgcg acggcggcat cgatgcgctc tacaccgcgc gcatgccgtc cacgtatcgc
      901 acgggcgggg gcaaggtgaa gcgcttgttc gagagctaca tggaggtcga gcaggcgtac
      961 taccgcaaga cgaggatctt tccgatcatg cacacggtcg cgatccgcag gagcctctac
     1021 gagaagcatc catgggtggc gatgtcgctc tacaaggcct tcgtgcaggc gcagcgcgag
     1081 acctacgacg atctctacgt gacggcggcc ctgaaggcga tgctgccgtg gctcacgaag
     1141 cacgtggaag acgcgcgcgc gctgatgggc gacgacttct ggccctacgg cttcgcgccc
     1201 aaccgcgaga cgctcgccac gttcctgcgc taccaccacg agcagggcct ttcgaagaag
     1261 ctcctcgcgc ccgaggagct gttcgcgccc gagacgatgg aaagcttcaa gatctgatgg
     1321 cggagcccct gggcaccaag aagcacacgc tcggcgacca tttgatgggc gagggcggcc
     1381 tgcacgcgca cgccgagggc gagtacgacc acgaccacga tcacgacgac ctgggcgagc
     1441 acgacctcga gaacgacgcg ttgtggcagc aggaccacat cacgctggtc tcggtcggca
     1501 tcgacatcgg ctcatcgggc acgcaagtca tcttctcgcg catctacatg cggcggctgg
     1561 ccgaagcgct caccagccgc tacttcgtcg tgaagcgcga gacgctctac cagtcgcccg
     1621 tcgccctcac gccttaccag agcgagacgc gcatcgacga gcgcgccgtg ggcgacatcg
     1681 tcgacgcggc gtacaccgcc gcgggcatac accccgacca gatcgacacc ggcgccgtga
     1741 tcctcaccgg agaggcgctg cgccgcgaga acgccaaagc catcgccgac gtgatcgccg
     1801 agatgggcgg cgagttc
//
LOCUS       DASVII010000243         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_659070,
            whole genome shotgun sequence.
ACCESSION   DASVII010000243 DASVII010000000
VERSION     DASVII010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659070"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..945
                     /locus_tag="VF309_03140"
     CDS             85..945
                     /locus_tag="VF309_03140"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7465610.1"
                     /translation="MRELKGRTAVVTGAGSGIGRELALACAREGMGVMLADIDESGLR
                     DTRAAVEALGVRAESIRCDVSNPLEVEALAERAWERFGAVHLLFNNAGVAVAGPTWTA
                     TLEDWKWTLGINLMGVVHGVRSFVPRMLAQGGECHVVNTASVAGLVDIPGSAVYCVSK
                     HGVVALSECLHHDLRVAGAAIGVSVLCPAYVNTAIGESARHRPSELAATNPLAAPYEE
                     AVRHALLHGKITAADVARATLDAVKSGRFYILTHEKIKGAIEARMRDILEDRTPTDVS
                     RPVPGAGARG"
     gene            967..1971
                     /locus_tag="VF309_03145"
     CDS             967..1971
                     /locus_tag="VF309_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7465611.1"
                     /translation="MSTTNRQVRLAARPTGLPKRSDWNFTEEAAGDPPDGQMLVKVTH
                     LSLDPAMRGWMNDGKSYIRPVAIGEVMRAGGAGKVVASKHPDYRVGDEVAGNFGVQQY
                     ALSDGKGVRKVDTRFAPLTTYLGALGMPGMTAYFGLLDIGKPKAGETVVVSGAAGAVG
                     SVVGQIAKIKGCRVIGIAGGADKCRYLTEKLHFDAAIDYKSEDVKAGLKTHCPDGIHV
                     YFDNVGGEILEAALGRIALHARIVICGAISQYNSTTPPKGPANYLSLLVNRASMTGMI
                     VFDYADRYAEGGREMAQWIAQGKLATKEDVVEGGIDAFPETLLKLFKGENFGKLVLAL
                     "
BASE COUNT          350 a          692 c          724 g          303 t
ORIGIN      
        1 cgacaaccat ccggatgccg aggaacgcat caaggccgcg gaagacgcgg cccgctagga
       61 cgcaacccag attggcggtg aagaatgcgt gaattgaaag gcaggacggc ggtcgtcacc
      121 ggcgcgggaa gcggcatcgg ccgcgagctg gcgctggcgt gcgcgcggga aggcatgggc
      181 gtcatgctcg ccgatatcga cgagtcgggc ctgcgcgaca cccgcgccgc ggtcgaggcg
      241 ctcggcgttc gcgcggaatc gattcgttgc gacgtttcga atccgctcga ggtcgaggcg
      301 ttggccgagc gggcatggga gcgcttcggc gccgtgcatc tcctgttcaa caacgcgggc
      361 gtggccgtgg ccggacccac atggaccgcg acgctcgagg actggaagtg gacgctcggc
      421 atcaacctga tgggtgtcgt ccacggagtg cgctccttcg tgccgcggat gctcgcgcag
      481 ggcggcgagt gccacgtcgt caacaccgcg tcggtcgcgg gcctcgtgga cattcccggc
      541 agcgccgtct actgcgtgag caagcacggc gtcgtggcgc tttccgagtg cctgcatcac
      601 gacttgcgcg tcgcgggtgc cgcgatcggc gtctcggtgc tgtgcccggc gtacgtgaat
      661 accgccatcg gggaatccgc gcgccatcgc ccgagcgagc tcgccgcgac caacccgctc
      721 gccgcgccct acgaggaagc ggtgcgccac gcgctgctcc atggaaagat caccgccgcc
      781 gacgtcgcgc gcgccacgct cgatgcggtg aagtccggcc gcttctatat cctcacccat
      841 gagaagatca agggcgcgat cgaggcgcgc atgcgcgaca ttctcgaaga tcgcacgccc
      901 accgacgtct cgcgtcccgt gcccggcgcg ggcgcccgag gctagcccac gcaaacagga
      961 ggatccatga gcaccacgaa tcgccaagtg cgcctcgccg cacgcccgac gggactgccc
     1021 aagcgcagcg actggaattt caccgaggaa gccgcgggcg atccgcccga cgggcagatg
     1081 ctggtgaagg tgacgcacct gtcgctcgac ccggcgatgc gcgggtggat gaacgatggc
     1141 aagtcgtaca tccgtcccgt cgcgatcggc gaggtgatgc gcgcgggcgg cgcgggcaag
     1201 gtcgtcgcct cgaagcatcc ggactatcgc gtgggcgacg aggtcgcggg caacttcggc
     1261 gtgcagcaat acgcgctctc cgacggcaag ggcgtgcgca aggtggacac gcgcttcgcg
     1321 ccgctcacca cttacctcgg ggcgctcggc atgccgggca tgaccgcgta cttcgggctc
     1381 ctcgacatcg gcaagccgaa ggccggcgag accgtggtcg tttccggggc ggccggcgcc
     1441 gtcggctcgg tcgtggggca gatcgcgaag atcaagggct gtcgcgtgat cggcatcgcg
     1501 ggcggcgcgg acaagtgccg ctatctcacg gagaagctcc acttcgacgc cgccatcgac
     1561 tacaagagcg aggacgtgaa ggccgggctc aagacccatt gccccgatgg catccacgtg
     1621 tacttcgaca acgtgggcgg cgagatcctc gaggccgcgc tcgggcgcat cgcgctgcac
     1681 gcgcgcatcg tcatctgcgg cgcgatctcg cagtacaaca gcacgacgcc gcccaagggg
     1741 ccggcgaact atctttcgct cctcgtgaac cgcgccagca tgaccggcat gatcgtgttc
     1801 gactacgccg atcgctacgc cgagggcggg cgcgagatgg cgcagtggat cgcgcagggc
     1861 aagctcgcca cgaaggagga cgtggtcgag ggcggcatcg acgcctttcc cgagacgctc
     1921 ctcaagctct tcaaggggga gaatttcggc aagctggtgc tcgcgctcta ggcctgcgat
     1981 gagacggatc ttcctggcca tgctcctcgc cgcgggcgcg agcgctctcg cgcaggcgcc
     2041 cgcgtacccc gacaagcccg tgaggatca
//
LOCUS       DASVII010000244         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_227752,
            whole genome shotgun sequence.
ACCESSION   DASVII010000244 DASVII010000000
VERSION     DASVII010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227752"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="dctP"
                     /locus_tag="VF309_03150"
     CDS             <1..422
                     /gene="dctP"
                     /locus_tag="VF309_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7465612.1"
                     /translation="QALQTGVVDGTENPPSNFFTQKMNEVQKFLTLSDHHYLGYAVIA
                     NKKFWDGLPPDIRKTLEGAMADATKYADDIAKKENDDALEAVKKAGKTEIITLTPAEK
                     LAWKRALVKVHKESESRVGKDLIESIYKETVFDPNKL"
     gene            433..1032
                     /locus_tag="VF309_03155"
     CDS             433..1032
                     /locus_tag="VF309_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7465613.1"
                     /translation="MLKVLDRLEEILIASLIAGATILIFVAVTHRYALDMSAKFHFDA
                     LYDALFKINLSWAQELCIYMFVWMAKFGAAYGVRTGIHVGVDVVINHLPPRWRFISVM
                     FGLLAGAFFTAVVGTLGVHFVYELSKTDQTSPDMEMPMWVVYLAIPCGSYLMSFRFLQ
                     VAWGFFRTGELPHHDAAHVEGVTEDAAIAPISAPIGATR"
     gene            1029..>1607
                     /locus_tag="VF309_03160"
     CDS             1029..>1607
                     /locus_tag="VF309_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7465614.1"
                     /translation="MSALPLPPSMTTKQAATALSLVAIMLLAVASLGNAGIIFAILVA
                     MMLTGMPISIALGLTVLTFLFTMTKVPIEAVGLKLFTGIEKFEIMAVPFFILAGNFLT
                     HGGVARRMIDFATSTIGHFHGGLALAGIVACAMFALVCGSSVATVVAIGSIILPEMVR
                     NGYPMRFGAGVITVAGSLGILMLPSIPKVIYAV"
BASE COUNT          273 a          531 c          508 g          295 t
ORIGIN      
        1 accaggcgct gcagacgggc gtggtcgacg gcaccgagaa cccgccgtcg aacttcttca
       61 cgcagaagat gaacgaggtg cagaagttcc tcacgctttc ggaccatcac tatctcggct
      121 acgcggtgat cgcgaacaag aaattctggg acggcctgcc gcctgacatc cgcaagaccc
      181 tcgaaggcgc catggccgat gccaccaagt acgccgacga catcgccaag aaggaaaacg
      241 acgacgcgct cgaagcggtg aagaaagccg gcaagaccga gatcatcacg ctgactcccg
      301 cggagaagct cgcgtggaag cgcgcgctcg tcaaggtcca caaggagagc gagtcacgcg
      361 tcggcaagga cctgatcgag tcgatctaca aggaaaccgt gttcgatccg aacaagctgt
      421 agggaggccg ccgtgctcaa ggtgctggac cgcctcgagg agatattgat cgcgtcgctc
      481 atcgcgggcg cgacgatcct catcttcgtg gcggtcacgc accgctatgc gctcgacatg
      541 agcgccaagt tccacttcga cgcgctgtac gacgcgctct tcaagatcaa cctgtcctgg
      601 gcgcaggagc tttgcatcta catgttcgtg tggatggcga agttcggcgc ggcctacggc
      661 gtgcgcaccg gcatccacgt gggcgtggac gtggtcatca accacctgcc gccgcgctgg
      721 cgtttcatct ccgtcatgtt cggcctcctc gcgggcgcct tcttcacggc cgtggtcggc
      781 acgctgggcg tgcacttcgt ctacgagctt tcgaagaccg accagacctc gcccgacatg
      841 gagatgccga tgtgggtcgt gtacctcgcg atcccctgcg gctcgtatct catgtcgttc
      901 cgcttcctgc aggtcgcgtg gggcttcttc cgcacgggcg agctgccgca ccatgacgcc
      961 gcgcacgtcg agggcgtcac cgaggacgcg gccatcgcgc ccatttcggc gcccatcgga
     1021 gcgacgcgat gagcgcgctg ccccttccgc cctccatgac cacgaagcag gccgcgacgg
     1081 cgctgtcgct cgtcgcgatc atgctgctcg cggtggcctc gctcggcaac gccgggatca
     1141 tcttcgccat cctcgtggcg atgatgctca ccgggatgcc gatctccatc gcgctcggcc
     1201 tcacggtgct caccttccta ttcacgatga cgaaggtgcc gatcgaggcc gtgggcctga
     1261 agctcttcac cggcatcgag aagttcgaga tcatggcggt gccgttcttc atcctcgcgg
     1321 gcaacttcct cacccacggg ggcgtggcgc ggcggatgat cgacttcgcg acctccacca
     1381 tcggccactt ccacggcggc ctcgcgctcg ccgggatcgt ggcgtgcgcc atgttcgcgc
     1441 tggtgtgcgg ctcgagcgtc gcgacggtgg tggcgatcgg ctccatcatt cttccggaga
     1501 tggtgaggaa cggctacccg atgcgcttcg gcgcgggcgt gatcacggtg gcggggtcgc
     1561 tgggcatcct gatgctgccc tcgatcccga aggtgatcta cgcggtg
//
LOCUS       DASVII010000245         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_659176,
            whole genome shotgun sequence.
ACCESSION   DASVII010000245 DASVII010000000
VERSION     DASVII010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659176"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VF309_03165"
     CDS             <1..1176
                     /locus_tag="VF309_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX7465615.1"
                     /translation="AQRRLVEQGFELRAWWLREMLVTPSPVTERMTLFWHNHFATSQQ
                     KVRLGQLMYRQNALLRREALGNFATMLHAVAKDPAMLVYLDNAGSRKQAPNENFAREV
                     MELFTLGEGHYGEQDVKEAARAFTGWSLDRETGEFTYRRAWHDYGEKTVLGKSGRFDG
                     DDVLDVLLARPEAGQFIASKLWREFVSPNPDPREVARWASIFREAHYEVKPLLRAILT
                     SDAFWSAENRGALIKSPVELVVGTLRTCGISPFDLRPAVFAAAALGQNPFSPPNVKGW
                     PGGDAWITSSTLLGRKQWLERVFRGSDPATMAMTEHGDDAKAAAPGERYRRMLERGMS
                     DYAFDVERFDRAIASAPGPARIDRLLLAADAVNALPDGLDSAERVRMLVADPAYQLR"
     gene            1173..2324
                     /locus_tag="VF309_03170"
     CDS             1173..2324
                     /locus_tag="VF309_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7465616.1"
                     /translation="MTMDRRAFLKLASLCPFAGAASVAWAAASPARKLFILVELKGGN
                     DGLNMVVPYADPEYARIRPRLAIPRDQVVQLSEQSGLHPSLAPLRAAWDEHRLAIVQG
                     VGYPQPNLSHFRSIEIWDTASKSDEYLDAGWLARAFAVSPSPDSYAADGVVVGPGAMG
                     PLAGPGARAIALTNPEQFLRNARLARAEGESRNAALAHILRVERDVMLSAERLHVGGK
                     LATEFPRNPFGNAANTAVQLASNEAGIAVVRLSLGSFDTHANQLGTHANLLKQLGEGL
                     AALREGLMEIDRWDSTVIATYAEFGRRPHENQSGGTDHGTSSAHLVLGGAVKGGFYGA
                     RPDLARLDGNGNLPFAVDFRGYYATFLERWWGIDSTKVLGARHAKLDFI"
BASE COUNT          382 a          805 c          784 g          376 t
ORIGIN      
        1 gcgcagcgcc gcctcgtcga gcagggcttc gagctgcgcg cctggtggct gcgggagatg
       61 ctcgtcactc cgagcccggt cacggagcgc atgacgctct tctggcacaa ccatttcgcc
      121 accagccagc agaaggtgcg cctcggccag ctcatgtacc ggcagaacgc gctgctgcgc
      181 cgcgaggcgc tcggtaattt cgcgaccatg ctccacgccg tcgccaagga tccggcgatg
      241 ctcgtctatc tcgataacgc cggcagccgc aagcaggcgc ccaacgagaa cttcgcccgc
      301 gaggtgatgg agctgttcac gctcggcgaa ggacattacg gagaacagga cgtgaaggag
      361 gcggcgcgcg ccttcaccgg gtggagcctc gaccgcgaga ccggggaatt cacctatcgg
      421 cgcgcgtggc acgactacgg cgagaagacc gtgctcggca agagcgggcg cttcgatggg
      481 gacgacgtgc tcgacgtgct cctcgcgcgc ccggaggccg ggcaattcat cgcgagcaag
      541 ctgtggcgcg agttcgtctc ccccaatccc gatccccgcg aagtcgcgcg ctgggcctcg
      601 atctttcgcg aggcgcatta cgaggtgaag ccgcttctcc gggcgattct cacgtcggat
      661 gcattctggt cggcggagaa tcggggcgcg ctcatcaagt cccccgtgga gctcgtagtg
      721 ggaacgctgc gaacctgcgg catcagcccg ttcgatctcc ggcccgcggt gttcgccgcg
      781 gccgcgctgg gccagaaccc cttctcgccg ccgaacgtga agggatggcc cggaggcgac
      841 gcgtggatca cttcctcgac gctcctcggg cggaagcaat ggctcgagcg cgtgtttcgc
      901 ggctccgacc cggcgaccat ggcgatgacc gagcacggcg acgatgccaa ggcggccgcg
      961 cccggcgagc gctatcgccg catgctcgaa cgcggcatgt ccgactatgc cttcgacgtg
     1021 gagcgcttcg accgcgcgat cgcgagcgcg ccgggccccg cgcggatcga tcgacttctc
     1081 cttgccgccg atgccgtgaa cgcgcttccc gacggcctcg attccgccga gcgggttcga
     1141 atgctcgtcg ccgaccccgc gtaccagctt cgatgaccat ggaccggcgc gcattcctga
     1201 agctcgcatc gctctgtcct ttcgccggcg cggcaagcgt cgcatgggcg gccgcatcgc
     1261 ccgcgcgcaa gcttttcatc ctggtggagc tgaagggcgg caacgacggc ctcaacatgg
     1321 tcgtccccta cgccgatccc gagtacgcgc ggatccgtcc gcgtctcgcg atcccgcgcg
     1381 accaggtcgt gcagttgagc gagcagtccg ggctgcatcc gtcgctcgcg ccgctgcgcg
     1441 cggcgtggga tgagcaccgg ctcgcgatcg tgcagggcgt gggttacccg caaccgaacc
     1501 tctcgcactt ccgctcgatc gagatctggg acaccgcgtc gaagagcgac gagtacctcg
     1561 acgcgggatg gctcgcgcgc gccttcgccg tgtcgccgtc acccgatagc tacgcggccg
     1621 acggcgtggt cgtcggaccc ggggcgatgg gaccgctcgc cggtccgggc gcgcgcgcca
     1681 tcgccctcac caatcccgag caattcctgc gcaatgcgcg ccttgcgcgg gccgaaggcg
     1741 agtcgcgcaa cgcggcgctg gcgcacatcc ttcgcgttga gcgcgacgtc atgttgtccg
     1801 ccgagcggtt gcacgtagga gggaagctcg cgacggagtt tcccaggaac cccttcggca
     1861 acgcggcaaa taccgcggtg cagctggcgt ccaacgaggc gggcatcgcc gtggtgaggc
     1921 tttccctggg aagcttcgac acgcacgcga accagctggg cacccacgcg aacctgctga
     1981 agcagctcgg cgaggggctt gcggcacttc gcgaggggct catggagatc gatcgctggg
     2041 actcgacagt gatcgcgacc tacgcggaat tcggccggcg cccccacgag aaccagagcg
     2101 gcggcaccga tcacgggact tcgagcgccc acctcgtgct gggcggtgcg gtgaaaggcg
     2161 gcttctatgg cgcgcgtccc gacctggcgc ggctcgacgg caatggcaac ctgcctttcg
     2221 ccgtcgattt ccgcggctac tacgccacat tcctggagcg ctggtgggga atcgattcca
     2281 ccaaggtgct cggtgcgcgg cacgccaaac tagacttcat ttaaaacatt agatatatac
     2341 aataacg
//
LOCUS       DASVII010000246         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_120041,
            whole genome shotgun sequence.
ACCESSION   DASVII010000246 DASVII010000000
VERSION     DASVII010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120041"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VF309_03175"
     CDS             complement(<1..854)
                     /locus_tag="VF309_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7465617.1"
                     /translation="MLARHLRFMLLLELAAYAGIAEWLHLRFGWSYGALAFLGIGAAL
                     AWRFAMVCVGASIAHAARSPRAAEHRIGPIGGLKLLLREWGAVVATSLFRFPWERYAL
                     RRDPEPGPATAIPIILVHGYLANRGYFASLVRRLESRGVAPIFTPNLPSSFATIERYA
                     EELGAEIERIAGAGGAQVVVIAHSMGGLGARAYLCAHGARRVRKLITIASPHHGTVHA
                     RFGAGASARQMSPGSRFLEELREREGARGPGCPVTSIYSPHDNLVAPQETSVLPWAKN
                     IAIPGRGHV"
     gene            947..>1808
                     /locus_tag="VF309_03180"
     CDS             947..>1808
                     /locus_tag="VF309_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465618.1"
                     /translation="MPLHAATVFLSAFLLFLVQPILAKQILPWFGGAAIVWTLCMVFF
                     QLVLLLGYAYAHWLASRVDGRRQAWIHVALLVASLAFLPVTPDASWKPVGGETPVWRI
                     LALLFATVGLPYFLLSSTSPLVQSWFARAFPGSSPYRLFALSNFASMIALLGYPFLFE
                     PWFTNQDQSLWWSVGYALFAMCCAALAVKCRALPALTVEGGQIAATALEPAPGARQIA
                     LWLALAAMGSVTLLGVSNHLTQNISSIPLLWVIPLAVYLLTFILCFEGRNWYRRDIYL
                     GSLAWVLCVMA"
BASE COUNT          236 a          649 c          610 g          313 t
ORIGIN      
        1 acgtggccgc ggccgggaat ggcgatgttc ttcgcccagg gcaggacgct cgtctcctgc
       61 ggcgcgacca ggttgtcgtg cggcgagtag atcgaagtca cgggacagcc tggcccccgg
      121 gcaccctccc tttcccgcag ctcctcgagg aagcggctgc cgggcgacat ctgccgcgcg
      181 ctggcgcccg cgccgaagcg cgcatgcacg gtgccgtggt gcgggctcgc gatggtgatg
      241 agcttgcgca cgcgccgggc gccatgggcg cagagatagg cccgcgcgcc gagccctccc
      301 atgctgtgcg cgatcacgac gacctgcgcg ccgcccgcgc cggcgattcg ctcgatctcg
      361 gcgcccagct cctcggcgta tcgctcgatg gtggcgaagc tcgaggggag attgggcgtg
      421 aagatgggcg ccacgccgcg cgactcgagg cgtcgcacca ggctcgcgaa gtacccgcga
      481 ttggcgagat agccgtgcac caggatgatc ggaatcgccg tggccggtcc cggctcgggg
      541 tcgcggcgca gggcgtagcg ttcccatggg aagcggaaaa ggctcgtcgc gacgacggcg
      601 ccccattcgc gaagcagcag tttcagcccg ccgatcggac cgatgcgatg ctcggccgcg
      661 cgcggcgacc tcgcggcgtg cgcgatggag gcgccgacgc aaaccatcgc gaagcgccag
      721 gcgagcgccg cgccgatgcc gaggaacgcc aacgccccgt agctccatcc gaaccgcaga
      781 tgcagccatt cggcgatccc cgcgtaggct gcgagttcga ggagcagcat gaaacgcagg
      841 tggcgcgcga gcatcgtccg ccagtgtagc gccgcgcggc gggctcgaag gcccgtgcta
      901 acattccgcg cacttccgcg ccccgcccac tccccgcatc ttccgcatgc cgcttcacgc
      961 cgcaaccgtc ttcctcagcg cattcctgct cttcctcgtg cagccgatcc tcgccaagca
     1021 gatcctgccc tggttcggcg gcgcggcgat cgtctggacg ctgtgcatgg tgttcttcca
     1081 gctcgtgctg ctgctgggat acgcctacgc gcactggctc gcgagccgcg tcgacggccg
     1141 gcgccaggcc tggatccacg tggcgctcct cgtcgcgagc ctcgctttcc ttcccgtgac
     1201 gccggacgcg tcgtggaagc cggtgggagg cgagacgccc gtgtggagga ttctcgcgct
     1261 cctcttcgcg acggtggggc tcccctactt cctcctgtcg tcgaccagcc cgctcgtgca
     1321 gtcctggttc gcgcgcgcct ttcccggctc gagcccgtac cggctcttcg cgctctcgaa
     1381 cttcgcttcg atgatcgcgc tcctgggcta tccgttcctc ttcgagccct ggttcacgaa
     1441 ccaggaccag tcgttgtggt ggtccgtggg ctacgcgctc ttcgcgatgt gctgcgccgc
     1501 gctcgcggtg aagtgccgcg cgcttcctgc cctgacggtg gaaggcggcc agatcgccgc
     1561 gaccgccctg gagccggcgc cgggcgcgcg gcagatcgcg ctctggctgg cgcttgcggc
     1621 gatgggctcg gtcacgctcc tcggggtctc caaccatctc acgcagaaca tctcgtcgat
     1681 tccgctcctg tgggtgatcc cgctcgcggt gtacctcctc acgttcatcc tgtgcttcga
     1741 gggccgcaac tggtaccggc gcgacatcta ccttggaagc ctcgcgtggg tcctgtgcgt
     1801 gatggcgt
//
LOCUS       DASVII010000247         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_83704, whole
            genome shotgun sequence.
ACCESSION   DASVII010000247 DASVII010000000
VERSION     DASVII010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83704"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF309_03185"
     CDS             complement(<1..258)
                     /locus_tag="VF309_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEX7465619.1"
                     /translation="MSDGARMNSELRGLLKHDEPMSKHVSWRAGGMARTFYQPADVLD
                     LATFLRTRASNEPVMFLGLGSNLLVRDGGFKGSVVFTHHALI"
     gene            complement(268..1686)
                     /gene="murC"
                     /locus_tag="VF309_03190"
     CDS             complement(268..1686)
                     /gene="murC"
                     /locus_tag="VF309_03190"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7465620.1"
                     /translation="MRHKVKRIHFVGIGGSGMSGIAEVFSSLGYTVSGSDLSENPAVR
                     RLRQMGIRIVIGHAAENVASADAVVVSSAVKADNPEVIRAREAHIPVVPRAMMLAELM
                     RFKQGIAIAGTHGKTTTTSLVAAVLGEAGLDPTCVIGGRLNSIGTNARLGKGEFLVAE
                     ADESDASFLYLQPVISVVTNIDADHMETYEQDFEKLKATFVEFLGHLPFYGLAVICKD
                     DPHVRAVMPAITRPMLTYGKTSDADLRAEGVRWDAGRMRFRAVATRGGFAPLEVLLNL
                     PGEHNVLNALAAIAVAREVGASDAAIAKGLAEFTGVGRRFQRYGDVPLLGGGLYTVVD
                     DYGHHPAEMAATLAAARGAFPGRRIVLAFQPHRYTRTRDLFEDFVKVLSTVDELVLAE
                     VYPAGEAPIVGADGRSLVRSVRLAGKVEPIFVEKTSEMRDAVLARVRDGDVVVTMGAG
                     SIANLAPELAIPAPGALAAVRS"
     gene            complement(1676..2788)
                     /gene="murG"
                     /locus_tag="VF309_03195"
     CDS             complement(1676..2788)
                     /gene="murG"
                     /locus_tag="VF309_03195"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX7465621.1"
                     /translation="MNRTILIMAAGSGGHIFPGLAIAKELVSRGWKVHWMGTPAGMEA
                     RLVKQAGYPMVTVNMSGVRGKGIAAWLALPLKILVAFWQSTVAIFRIRPDVVMSMGGY
                     VAFPGGMMAALWGKPLVVHEPGAVAGITNRVLAPVAERVIVGMEGAFERRVAQRWANL
                     LPAPRRVAWLGTPVRDEIAAVPAPEERYAGRSGPLRLLVVGGSLGAQTMNDLVLAAVA
                     SMPPGDRPEIVHQSGEKLFEGLRSGYARESVRAEVLPFVEDMAARYAWCDVLVCRSGA
                     ITVAEITAAGVASILFPLPWFVADEQAANARFLAGRDAGIALKQLETQPAQLAQILRG
                     LDRNRLMAMARNARALGKPDATRRCADVCMELAHAA"
BASE COUNT          472 a         1020 c          975 g          421 t
ORIGIN      
        1 gatcaaggca tggtgagtga agaccaccga gcccttgaac cctccatcgc gcacgaggag
       61 gttgcttccg aggccgagga acatcaccgg ttcattcgaa gcgcgcgtgc gcaggaacgt
      121 cgcgaggtcg agcacgtcgg cgggctggta gaacgtgcgc gccattccgc cggcgcgcca
      181 gctcacgtgc ttcgacatcg gctcgtcgtg cttcaagagt ccccgcagct ccgaattcat
      241 tcgcgcgccg tcgctcatgt ccatccccta gctccgcacc gcggccagcg cgcccggcgc
      301 cggaatcgcc agctcgggcg cgaggttcgc gatcgagccc gcgcccatcg tgacgacgac
      361 gtcgccgtcg cggacccggg cgagcaccgc gtcgcgcatc tcggaagtct tctcgacgaa
      421 gatcggctcg accttgccag caaggcgcac cgaccggacc agggagcgtc catcggcccc
      481 gacgatcggc gcctcccccg cgggatacac ctccgcgagg accagctcgt cgacggtcga
      541 gagcaccttc acgaaatcct cgaagagatc gcgcgtgcgc gtgtagcggt gcggctggaa
      601 ggcgagcacg atgcgccgcc ccggaaacgc gccgcgcgcg gccgcgaggg tcgccgccat
      661 ttccgccggg tggtggccgt agtcgtcgac gaccgtgtag aggccgccgc cgaggagcgg
      721 cacgtcgccg tagcgctgga agcgtcgccc cacgccggtg aattcggcga gccccttcgc
      781 gatcgccgcg tcgctcgcgc cgacctcgcg cgccacggcg atcgccgcca gggcattcag
      841 cacgttgtgc tcgcccggaa ggttcagcag cacctcgagc ggcgcgaagc cgccgcgcgt
      901 cgctaccgcg cggaagcgca tgcgccccgc gtcccagcgc acgccttccg cgcgcaggtc
      961 ggcatcgctc gtcttgccgt aggtgagcat cgggcgcgtg atcgccggca tcacggcgcg
     1021 cacgtgcgga tcgtccttgc agatcaccgc gaggccgtag aacggcaggt ggccgaggaa
     1081 ttcgacgaag gtcgccttga gcttctcgaa atcctgctcg taggtttcca tgtgatcggc
     1141 gtcgatgttg gtgacgacgg aaatcaccgg ctggagatag aggaacgagg cgtccgactc
     1201 gtcggcttcg gcgacgagaa actcgccctt gccgaggcgc gcattggtgc cgatggagtt
     1261 gagccgcccg ccgatcacgc acgtcggatc gagcccggct tccccgagca ccgcggcgac
     1321 gagcgaagtc gtcgtcgtct ttccatgggt gcccgcgatg gcgatgccct gcttgaagcg
     1381 catcagctcc gcgagcatca tcgcccgcgg cacgaccggg atatgggcct cgcgggcccg
     1441 gatcacttcg gggttgtcgg ccttcaccgc cgaggagacg acgaccgcgt ccgccgaggc
     1501 gacgttctcc gcggcatggc cgatcacgat gcggatgccc atctggcgca gcctgcgcac
     1561 cgcggggttc tccgacaggt ccgagcccga caccgtatag ccgaggctgg agaacacctc
     1621 cgcgatcccg gacatgcccg agccgccgat gccgacgaaa tggatgcgct taaccttatg
     1681 ccgcatgggc aagctccatg cacacgtcgg cgcagcgccg cgtcgcgtcg ggttttccga
     1741 gggcgcgcgc gttgcgcgcc atggccatga ggcggtttct atcgagtcca cgaaggatct
     1801 gggcaagttg ggcgggttgg gtttcgagct gcttcaatgc gattcccgcg tcgcgcccgg
     1861 cgaggaagcg cgcgttcgcg gcctgctcgt cggcgacgaa ccacggcagg ggaaagagga
     1921 tcgaggcgac gccggcggcg gtgatctcgg cgacggtgat cgcgcccgag cgacagacga
     1981 gcacgtcgca ccacgcgtat cgcgccgcca tgtcctcgac gaagggcagc acctcggcgc
     2041 gcacgctttc cctcgcgtag cccgagcgca gtccctcgaa gagtttttcg cccgactggt
     2101 ggacgatctc cgggcgatcg ccgggaggca tcgaggccac ggcggcgagg acaaggtcgt
     2161 tcatcgtctg cgcgccgagg cttccgccga cgacgaggag ccgcagcggc cccgagcgtc
     2221 ccgcatagcg ctcctcgggc gccggcaccg cggcgatctc gtcgcgcacc ggcgtgccga
     2281 gccaggcgac gcggcgcggt gcgggcagga gattggccca gcgctgggcg actctccgct
     2341 cgaacgcgcc ttccatcccg acgatcaccc gctccgcgac gggagcgagc acgcgattgg
     2401 tgatgcccgc gacggcgccg ggctcgtgca ccacgagggg cttgccccag agcgccgcca
     2461 tcatgccgcc ggggaacgcg acgtagccgc ccatggacat caccacgtcg ggacggatgc
     2521 ggaagatggc gaccgtcgac tgccagaacg ccaccaggat cttcaacggc aacgcgagcc
     2581 acgcggcgat gcccttgccg cgcacgccgc tcatgttcac cgtgaccatg ggatagcccg
     2641 cctgcttcac gagcctcgcc tccatgcccg cgggagtgcc catccagtgc accttccagc
     2701 cgcgcgagac gagctccttc gcgatcgcga gccccggaaa gatgtgcccg ccggagcccg
     2761 ccgccatgat gagaatggtt cgattcacag cgaacccccg cgcatcaggt agcgattttc
     2821 ccagtcgatg cgcatgagga tgcccagcgc catgcaggtg accacgatgc cggtgccgcc
     2881 gaacgaca
//
LOCUS       DASVII010000248         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_48054, whole
            genome shotgun sequence.
ACCESSION   DASVII010000248 DASVII010000000
VERSION     DASVII010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48054"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1771
                     /locus_tag="VF309_03200"
     CDS             <1..>1771
                     /locus_tag="VF309_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7465622.1"
                     /translation="RAEQTNLGRTVAVKIIHPHLVGEENAAARFITEARAASRLNHPH
                     SVGVIDFGKTDDGQLYLVMEFLRGRDLARVAYEDGPLPIRRILDVLRQALAALAEAHH
                     IGIVHRDLKPENIILEPLRSGGDFVKVVDFGLAKMVEGPRQPAITTPGIICGTPDYMA
                     PEQGRGAVIDARTDLYAVGVILFQLLTGRLPFEADTPTQVVLMHLSIPAPDPRQVAPE
                     RNIPDALAKIVAKALAKEPEDRWTTADDLSEALLSVQGEIEIKSRSASMLPQMNCPSC
                     NEPVPRGQKFCGECGARVSIPPLSRPPPSSRGERAAVQVAPKLPLPLTHREEDVAFLE
                     HYRSELRSSLSAVRIVGEAGVGKTRLLREFLARARNAGDRVVETGPDPWWAEVGYYAA
                     RRAIAALAELAPNGGEERDWSGASAEARRGLIEVFGRVDALAATLSPSERRYTAAEAL
                     RWALTRAAAKAGKAKVILAIDDLNRVDGASRNAFADALAEPPLVPVLIVAAHAPPFDP
                     GWGGSAVRPIAGLPASKAAELVRSYGVGGERLSLLPGDHAPSSPPVLPLYLDQLVRFT
                     TEGGGDPPARLADLVALRIERLEP"
BASE COUNT          277 a          652 c          563 g          279 t
ORIGIN      
        1 cgcgccgagc agacgaacct cggtcgcacc gtcgccgtca agatcatcca tccccacctc
       61 gtcggcgagg agaacgcggc ggcccggttc atcacggagg cgcgcgcggc gagccgcctg
      121 aaccacccac actcggtggg cgtcatcgac ttcggcaaga ccgacgacgg ccagctttac
      181 ctggtcatgg agttcttgcg cgggcgcgat ctggcacgtg tcgcctacga agatggtccg
      241 ctccccatcc gccgcatcct cgatgtgctc cgtcaggcgc tcgccgccct cgccgaagcc
      301 caccacatcg gcatcgtgca ccgcgatctc aagccggaga acatcatcct cgagccgctt
      361 cgctcgggag gagacttcgt caaggtggtg gacttcggtc tcgccaagat ggtcgagggg
      421 ccgcgccagc ccgccatcac gacgcccggc atcatctgcg gcacgcccga ttacatggcc
      481 cccgagcaag ggcgtggtgc cgtcatcgac gcgcgcaccg atctctacgc ggtgggcgtc
      541 atcctttttc agctcttgac cggaagattg cccttcgaag ccgacacgcc gacacaggtg
      601 gtgctcatgc acctctcgat ccccgcgccc gatccgcggc aggtcgcccc ggagcgcaac
      661 atccccgacg cgctcgccaa gatcgtcgcc aaggcgcttg ccaaggagcc cgaggatcgt
      721 tggacaacag ccgacgacct cagcgaagcg ctcctgtcgg tgcagggcga gatcgagatc
      781 aagagtcggt cggcctcgat gctgccgcag atgaattgcc cgtcgtgcaa cgagccggtg
      841 cctcgcgggc aaaagttttg cggagaatgc ggggcgcgcg tcagcattcc gccgctgtcg
      901 aggccgccgc cctcctcacg aggtgagcgc gccgcggttc aggttgcgcc caagctccct
      961 ctgccgctca cccaccgcga agaggacgtc gcatttctcg agcactaccg cagcgagctg
     1021 cgttcctcct tgtctgcggt gcgcatcgtg ggcgaagccg gggtcggcaa gaccaggctc
     1081 cttcgcgagt tcctcgctcg agctcgcaac gcaggcgatc gggtcgtcga aacaggcccc
     1141 gacccgtggt gggcggaagt cggctactac gccgcgcggc gcgcgattgc cgcactcgcc
     1201 gagctcgcgc ccaatggtgg cgaagaacga gactggagcg gcgccagtgc cgaagcccgt
     1261 cgcggactca tcgaagtatt cgggcgcgtc gatgcgctcg ctgcgacgct ctcgccgagc
     1321 gagcgccggt acaccgccgc cgaggcgctg cgctgggcgc tcacccgtgc ggcagcaaaa
     1381 gccggcaagg ccaaagtgat cctcgccatc gacgacctga atcgcgtcga cggcgcgagc
     1441 cgcaacgcct tcgccgatgc cctcgccgaa cctccgctcg ttcccgttct catcgttgcg
     1501 gctcacgcgc cgccgttcga cccagggtgg ggaggcagcg cagttcgacc cattgccggt
     1561 ctccccgcgt ccaaggcggc ggagctcgtg cgctcttacg gcgtcggggg cgagcgcctc
     1621 tcgcttctgc ccggcgacca cgcgccgagc tcgcctcccg tgcttcccct ctacctcgac
     1681 cagctggttc ggttcaccac cgagggcggc ggtgatccgc cggcgcgcct ggccgacctc
     1741 gtcgccctgc gcatcgagcg tctcgagccc g
//
LOCUS       DASVII010000249         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_515256,
            whole genome shotgun sequence.
ACCESSION   DASVII010000249 DASVII010000000
VERSION     DASVII010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515256"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF309_03205"
     CDS             complement(<1..720)
                     /locus_tag="VF309_03205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7465623.1"
                     /translation="MHPMLNTAVKAARKAGSIISRASSDLDRLTVRSKRQNDFVSEVD
                     HAAEEAIIAVLREAYPSHGILAEESGSRDADAEYVWVIDPLDGTTNFLHGFPQYCVSI
                     GLLHKGAPQQAVVFDPNRNELFTATKGVGAYLNDRRVRVSKTDRLENALMGTGFPFRE
                     VGHLDDYLRMFKNVTLNCAGVRRPGDAALDLAWVAAGRIDGFWEIGLSPWDIAAGALI
                     VSEAGGMVGRIDGAPYDTSVID"
     gene            819..1550
                     /locus_tag="VF309_03210"
     CDS             819..1550
                     /locus_tag="VF309_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7465624.1"
                     /translation="MDEFPLHERLAVVLSRPSHPGNIGGAARAMKTMGLSDLRVVAPK
                     RFPDPEAVALASGAADVLEGARVFERLEDAIADSILAVGFSSRPRDLSHPPLALREAA
                     PAIVDACARGRVALVFGNETFGLTNEELARCQMLATIPANPAYASLNLAAAVQVACYE
                     VAVTATLHAPAQGVMHESATGAEIEALFAHWEAAMVASGFLDPAQPKRLMERMRRLFA
                     RAGLEREEVKLLRGMLAAFEKGRGG"
     gene            complement(1547..>2494)
                     /locus_tag="VF309_03215"
     CDS             complement(1547..>2494)
                     /locus_tag="VF309_03215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7465625.1"
                     /translation="VQPIALLGILACANLRLPFAAIAACATLAGAFATPITTLTRTSW
                     RYRFESEEDRRTAFALDSVMIEINFTLGPAIVAGILATLGAAVAFGFAIAVVVISFVI
                     FFASPALRYFKRSGPVVRSLLGPLAEPRLVLLFVATFGNALCYGLLEVGYPAYGTSLG
                     LPALAGILLAVNSLGSALGGALFGGLHFKASVERQFACVMALMAVPVLLHAFVSTPLA
                     FGVVAFLAGALIAPSIAAQSVLVSRLAPSHYATEAFTWSSTFIVSGLGSGMAPGGFLV
                     ETSSLASVWVTGASVVGTMAAVAFVVTAPRAAKSARAGP"
BASE COUNT          412 a          823 c          913 g          346 t
ORIGIN      
        1 gtcgatgacc gaggtgtcgt agggggcacc gtcgatgcgc cccaccatcc cgccggcctc
       61 ggagacgatg agcgcgcccg cggcgatgtc ccacggcgac aggccgattt cccagaagcc
      121 gtcgatgcgt ccggccgcga cccacgcgag atcgagcgcc gcgtcgccgg gacgccgcac
      181 gcccgcgcag ttgagggtga cgttcttgaa catgcgcagg tagtcgtcga ggtgccccac
      241 ttcgcggaac ggaaatcccg tgcccatgag cgcgttctcg aggcggtccg tcttgctcac
      301 gcgaacgcgc cggtcgttga gataggcgcc cacgcccttg gtcgcggtga agagctcgtt
      361 gcgattgggg tcgaagacga ccgcttgctg cggcgcgccc ttgtgcagga gcccgatcga
      421 cacgcagtac tgcggaaagc cgtggaggaa gttggtggtg ccgtcgagcg gatcgatcac
      481 ccagacgtat tcggcgtccg catcgcgcga gccgctttcc tcggcgagga tcccgtgcga
      541 gggataggcc tcgcgcagca ccgcgatgat cgcctcctcc gccgcgtgat cgacctcgga
      601 gacgaaatcg ttctggcgct tgctgcgcac cgtgagcctg tcgagatcgg aggaggcgcg
      661 gctgatgatg ctgcccgcct tgcgcgcggc cttgacggcc gtgttgagca tgggatgcat
      721 ggaaggggcg tggggctcga ggcggccgca gggcttgcgc ggcgcgatgc ggcgatttta
      781 ctcgggatcg gggattcgat tccggcacaa tcgcccccat ggatgaattt cccctgcacg
      841 agcgcctcgc cgtcgtgctt tcgcgcccga gccatccggg caacatcggc ggcgccgcgc
      901 gcgcgatgaa gaccatggga ctgtcggacc ttcgcgtggt ggcgccgaag cgctttcccg
      961 atcccgaagc cgtggcgctc gcgagcggcg ccgcggacgt gctcgaaggc gcgcgcgtct
     1021 tcgaaaggct cgaggatgcg atcgcggatt cgatcctcgc cgtggggttc agctcgcggc
     1081 cgcgcgatct ctcgcacccg ccgctcgcgc ttcgcgaggc ggcgcccgcg atcgtcgacg
     1141 catgcgcgag agggcgcgtg gctctcgtct tcggcaacga gacctttggc ctcaccaacg
     1201 aagagctggc gcgctgccag atgctcgcga cgattcccgc caatcccgcc tatgcgtcgc
     1261 tcaatctcgc cgcggcggtc caggtcgcat gctacgaagt ggcggtcacc gccacgctgc
     1321 acgctcccgc gcagggagtc atgcacgaaa gcgccacggg cgcggagatc gaagcgctct
     1381 tcgcgcattg ggaggcggcg atggtggcga gcggcttcct cgatcccgcg cagcccaagc
     1441 ggctcatgga gcgcatgcgc aggctcttcg cccgcgccgg cctggaacgc gaggaagtga
     1501 agctgctgcg cggcatgctc gccgcgttcg agaaaggccg cggcggctag gggccggccc
     1561 gcgcggattt cgcggcgcgc ggcgccgtga cgacgaaggc gaccgccgcc atcgtcccca
     1621 cgaccgacgc gccggtcacc cacacggatg ccaggctgga ggtctcgacg aggaagccgc
     1681 cgggcgccat gcccgagccg agcccgctca cgatgaaggt cgaggaccac gtgaacgctt
     1741 ccgtcgcgta gtgggacggc gcgaggcgcg acaccagcac cgattgggct gcgatcgacg
     1801 gcgcgatgag cgccccggcg aggaacgcca ccacgccgaa cgcgagaggc gtgctcacga
     1861 atgcgtggag cagcaccggg accgccatga gcgccatcac gcaggcgaac tggcgctcga
     1921 cgctcgcctt gaaatgcagg ccgccgaaaa gagccccgcc gagggcgctg ccgagcgagt
     1981 tcacggcgag gaggatgccg gcgagcgcgg gcagccccag cgacgtgccg taggcggggt
     2041 agccgacctc gaggagaccg tagcaaagcg cgttgccgaa agtcgcgacg aagagcagga
     2101 cgagccgggg ttcggcgagc gggcccagga ggctgcgaac gaccgggccg cttcgcttga
     2161 agtagcgcag cgccggcgac gcgaagaaga tcacgaacga gatgacgacg accgcgatcg
     2221 cgaagccgaa ggccacggcc gcgccgagcg tcgcgaggat gcccgccacg atcgcggggc
     2281 cgagggtgaa gttgatctcg atcatcaccg agtcgagcgc gaacgccgtg cggcgatcct
     2341 cctcgctttc gaaccggtag cgccaggagg tgcgggtgag ggtcgtgatg ggcgtggcga
     2401 aagctccggc gagcgtcgcg caggccgcga tcgcggcgaa tggcaggcgc aggttcgcgc
     2461 aggcgaggat tccgaggagc gcgatgggct ggac
//
LOCUS       DASVII010000250         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_83799, whole
            genome shotgun sequence.
ACCESSION   DASVII010000250 DASVII010000000
VERSION     DASVII010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83799"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1918
                     /locus_tag="VF309_03220"
     CDS             <1..>1918
                     /locus_tag="VF309_03220"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7465626.1"
                     /translation="LVLAGDYPEALAVARQQVESGAQVIDINMDEAMLDSAAAMTRFL
                     QLAAAEPDISRVPVMIDSSKWSVIEAGLKCVQGKSVVNSISLKAGEAEFIHQAKLARR
                     YGAAVVVMAFDEKGQADSLKRKTEICARCYRILTEDVGFPPEDIIFDPNIFAVATGIE
                     EHNTYGRDFIEAVGWIHRSLPHAKTSGGVSNFSFSFRGNDPVREAMHTAFLYHATKAG
                     MTMGIVNAGQLGVYADIPAELLERVEDVILNRRPDATERLVEFAESFKGKKKDAVEDL
                     AWRKGTVRERLVHALVRGVTQHVIEDTEEARLQYERPLQVIEGPLMDGMNVVGDLFGS
                     GKMFLPQVVKSARVMKQAVAHLIPYIEEEKRRLGTAPKPKGKVVMATVKGDVHDIGKN
                     IVGVVLQCNNFDVVDLGVMVACDTILETARKERADMIGLSGLITPSLEEMSHVAKEME
                     RLGFTMPLLIGGATTSRTHTAVKIEPHYSGPTVWVPDASRAVGVASALASEDNRDKYM
                     VDVRADYVKVREAHAKKTGQKSVTLEAARANAAKIDWRGYGPPKPRQPGLASLKNYPL
                     EKLVPFIDWAPFFQTWDLAGKYPDILEDPVIGEAARNVFRDGKAMLDRVVKGRWLTAS
                     GVFGLWPANSVGDDI"
BASE COUNT          356 a          623 c          648 g          291 t
ORIGIN      
        1 ctcgtcctcg cgggcgacta tcccgaggcg ctcgccgtcg cgcgccagca ggtcgaaagc
       61 ggcgcgcagg tgatcgacat caacatggac gaggcgatgc tcgattcggc cgcggccatg
      121 acgcgcttcc tgcagctcgc cgccgccgag cccgacatct cgcgcgtgcc ggtgatgatc
      181 gacagctcca agtggtcggt catcgaagcg ggcctgaagt gcgtgcaggg caagtcggtg
      241 gtgaactcga tctcgttgaa ggcgggcgag gcggaattca tccaccaggc gaagctcgcg
      301 cgccgctacg gcgccgcggt ggtggtgatg gcgttcgacg agaagggcca ggccgattcg
      361 ctcaagcgca agaccgagat ctgcgcgcgc tgctacagga tcctcaccga ggatgtcggc
      421 ttcccgcccg aggacatcat attcgatccc aacatcttcg cggtcgccac cggcatcgag
      481 gagcacaaca cctacgggcg cgacttcatc gaggcggtcg gctggatcca ccggagcctc
      541 ccgcacgcga agacttccgg cggcgtgtcg aacttctcgt tctcgttccg cggcaacgat
      601 ccggtgcgcg aggcgatgca caccgcgttc ctctaccacg cgaccaaggc cggaatgacg
      661 atgggtatcg tcaacgcggg ccagctcggc gtgtatgccg acattcccgc ggagctcctc
      721 gagcgcgtgg aggacgtgat cctcaaccgg cgccccgatg cgaccgagcg cctggtcgaa
      781 ttcgccgagt cgttcaaggg aaagaagaag gacgccgtcg aggatctagc gtggcgcaag
      841 ggcacggtgc gggagcggct cgtccacgcg ctcgtccgcg gcgtgacgca gcacgtgatc
      901 gaggataccg aagaggcgcg gctccagtac gagcgcccgc tccaggtgat cgaaggcccg
      961 ctcatggacg gcatgaacgt cgtgggcgac ctcttcggct cggggaagat gttcctcccg
     1021 caggtcgtca agtcggctcg cgtcatgaag caggcggtgg cgcatctcat cccgtacatc
     1081 gaggaagaga agaggcgcct cggcaccgcg cccaagccca agggcaaggt cgtcatggcg
     1141 accgtgaagg gcgacgtgca cgacatcggc aagaacatcg tcggcgtcgt tctccaatgc
     1201 aataacttcg acgtcgtcga cctgggagtg atggtcgcgt gcgacaccat cctcgagacc
     1261 gcgaggaagg agcgcgccga catgatcggc ctgtcggggc tcatcacgcc ttccctcgag
     1321 gagatgtcgc acgtcgcgaa ggagatggag cggctcgggt tcacgatgcc gctcctcatc
     1381 ggaggcgcga ccacctcgcg cacccacacc gccgtcaaga tcgagcccca ttattccgga
     1441 ccgacggtct gggtcccgga cgcttcgcga gccgtgggcg tcgcatcggc cctggcgagc
     1501 gaagacaacc gagacaagta catggtggac gtgcgcgccg actacgtgaa ggtgcgtgag
     1561 gcgcacgcga agaagaccgg ccagaagagc gtgacgctcg aagcggcgcg cgccaacgcg
     1621 gcgaagatcg actggagagg atacggcccg ccgaagccgc ggcagccggg cctcgcgtcg
     1681 ctgaagaact atccgctcga gaagctcgtc cccttcatcg actgggcgcc cttcttccag
     1741 acgtgggacc tcgcgggcaa gtatcccgac atcctcgagg accccgtcat cggcgaggcc
     1801 gcgcgcaacg tcttccgcga cggcaaggcg atgctcgatc gcgtcgtcaa gggccgctgg
     1861 ctcacggcga gcggcgtctt cgggttgtgg cccgcgaatt cggtcggcga cgacatcg
//
LOCUS       DASVII010000251         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_299664,
            whole genome shotgun sequence.
ACCESSION   DASVII010000251 DASVII010000000
VERSION     DASVII010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299664"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VF309_03225"
     CDS             complement(<1..419)
                     /locus_tag="VF309_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7465627.1"
                     /translation="MFSFVSKYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     GGPNGKKTIQAVGLAAKQMLGRTPGNITAIRPMKDGVIADFTITEQMLKYFIKKIHDN
                     QWFRPSPRIVICVPYGSTQVERRAIKESAVGAGASQV"
     gene            522..806
                     /gene="gatC"
                     /locus_tag="VF309_03230"
     CDS             522..806
                     /gene="gatC"
                     /locus_tag="VF309_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7465628.1"
                     /translation="MLSLEEIHRLARLARLELSAAEADDMRTQINGILAMVDEMRAVD
                     TTGVAPMSHPQEVTQRLREDRVTETDQRELFHSLAPEVEDGLYLVPRVIE"
     gene            838..>1742
                     /locus_tag="VF309_03235"
     CDS             838..>1742
                     /locus_tag="VF309_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7465629.1"
                     /translation="MHNATLTELSRRLHAREVSSVEVAGCFLERIRAANAQLNAFITV
                     DEEKTLAQARIADARIAQGGAHRLAGIPIAHKDLFCAKGWRTTCGSKILENFVAPYDA
                     HVIERFNAAGAVILGKTNMDEFAMGSSNENSHFGPVKNPWDARAVPGGSSGGSAAAVA
                     SRIAPAATGTDTGGSIRQPASFTGVSGLRPTYGRVSRYGMVAFASSLDQGGALAKSAE
                     DLAMMLDVMAGFDERDSTCMDRAKDDYVGGLARGVKGLRIGVPREYFTKDLARDVGDP
                     ILAAIEAFKGQGAIVVEVSLPNQHLS"
BASE COUNT          306 a          560 c          592 g          284 t
ORIGIN      
        1 aacacctgcg acgcgccggc gccaacggcg ctttccttga tcgcgcgccg ctcgacctgc
       61 gtggacccgt agggcacgca gatcacgatc cgcgggctcg ggcggaacca ctggttgtcg
      121 tggatcttct tgatgaagta cttgagcatc tgctcggtga tcgtgaagtc ggcgatcacg
      181 ccgtccttca tcgggcggat ggcggtgatg ttcccgggcg tgcgcccgag catctgtttc
      241 gcggcgaggc ccacggcttg aatggtcttc ttgccgttgg gtccgccttc ctgccgtatt
      301 gccacgaccg agggttcgtc gaggacgatc cccttgccgc gaacgtagat cagggtgttg
      361 gccgtgccga ggtcgatcgc gaggtcggtg gaaaagtact tggaaacgaa ggaaaacatc
      421 ttgatccgta aggagtttcg gtttgcggcg ggtccccgag gaggggctgc aatataatag
      481 cccaaaatcg cgctgaatta aggccttagc agcactttcc gatgctatcc ctcgaagaaa
      541 tccaccgcct cgcccgcctg gcgcgactcg agctttcggc cgccgaggcc gacgacatgc
      601 gcacgcagat caacggcatc ctcgcgatgg tcgacgagat gcgcgccgtc gacacgacgg
      661 gcgtcgcgcc gatgtcgcat ccgcaggaag tcacgcaacg gctgcgcgaa gaccgcgtga
      721 ccgagaccga ccagcgcgag cttttccatt cgctcgcgcc cgaagtggag gacggcctct
      781 acctggttcc cagggtgatc gaatagaaga agccaggcac ggggcgcgag acaagagatg
      841 cacaacgcaa cattgaccga gctgtcgcgg cgccttcacg cgagggaagt ctcgagcgtg
      901 gaggtcgcgg gatgctttct cgagcggatc cgcgcggcca acgcgcagct gaacgcgttc
      961 atcaccgtgg acgaggagaa gacgctcgcg caggcgcgca tcgcggacgc gcgcatcgcg
     1021 cagggcggcg cgcaccggct cgccggcatt ccgatcgcgc acaaggacct cttctgcgcg
     1081 aagggatggc gcaccacctg cggctcgaag atcctcgaga atttcgtcgc gccctacgat
     1141 gcgcacgtga tcgagcggtt caacgccgcc ggcgcggtga tcctcggcaa gaccaacatg
     1201 gacgagttcg ccatgggctc gtcgaacgag aactcgcact tcggccccgt gaagaatccc
     1261 tgggacgcga gggcggtgcc gggcgggagc tcgggcggtt ccgccgccgc ggtcgcctcg
     1321 cgcatcgcgc cggccgcgac gggcaccgac accgggggct cgattcgcca gcccgcgtcg
     1381 ttcaccggcg tgagcggcct gcgccccacg tatggccgag tttcgcgcta cggcatggtc
     1441 gccttcgcct cgtcgctcga ccagggcgga gcgctcgcca agagcgccga agacctcgcg
     1501 atgatgctcg acgtgatggc gggcttcgac gagcgcgatt cgacctgcat ggaccgcgcc
     1561 aaggacgact acgtgggagg cctcgcgcgc ggcgtgaagg gcctgcggat cggcgtgccg
     1621 cgcgagtact tcacgaagga cctcgctcgc gacgtgggcg atccgatcct cgccgcgatc
     1681 gaggcgttca agggacaggg cgcgatcgtg gtggaggtga gcctgcccaa ccagcacctg
     1741 tc
//
LOCUS       DASVII010000252         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_335809,
            whole genome shotgun sequence.
ACCESSION   DASVII010000252 DASVII010000000
VERSION     DASVII010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335809"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1108)
                     /locus_tag="VF309_03240"
     CDS             complement(152..1108)
                     /locus_tag="VF309_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465630.1"
                     /translation="MRWIAIAFWMLAGASIPAFAQDSKAWLESQGLEAVDSKSFNEFE
                     AVVARPKGGGEQHALIFGKGGKPAWQSNPKETDPGSKWTIQSIGRDLAGNGQPTVHFS
                     SNTGGANCCTTHHVYQLKPQVKRLAVYSAGSMGGGDFIEVPGRKAPVMVSADDSSANA
                     FAPYANSYFPLLILEVGPKGRLQFAQDLMQSRLPGQPPPVCAQPLASANPWLKERCAE
                     YTSARRQARTAEIKSRLNTIKANRSADKLKWEDYFENGVLAGVSAEINRYTYTGHGAA
                     GFNWLETVWPGNDAVKAKFVATLRQTQAKSVFAEDLKSIATN"
     gene            complement(1160..>1904)
                     /locus_tag="VF309_03245"
     CDS             complement(1160..>1904)
                     /locus_tag="VF309_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7465631.1"
                     /translation="LPLGTVDIIYTGTAFAGRAYPPISIAAGPFIFRDYDHWQKFRNG
                     PVFKDLARGYEEKTGGAKILGYTYYGLRHLTANKMVTKPEDMKGMKLRVPDAPLFVMF
                     PRAVGANPTPIAFAEVYLALQNGTVDAQENPLPTIDAKKFYEVQKYIILTGHITESLV
                     TVMGAGTWAKLNDADRAIFSEVLWEASSRATQEIYDAENRLVTEFEKRGKTVVKVDRR
                     PFMQAVQKAVTSPDAPWPRELYDRVEAIR"
BASE COUNT          285 a          662 c          604 g          353 t
ORIGIN      
        1 ccgaaggcgt cgagaccgag gagcagctgg aggagctcaa gcgcctcggc tgccaccgtg
       61 cccagggctt ccttctcgcg cggcccatga ccggcgacgc catcgcgcgc ttcctcggcc
      121 gcgccgcctc cgccgccgcc gaatagcttt cctagttcgt ggcaatgctc ttcaggtctt
      181 cggcgaagac gctcttcgcc tgggtctggc gcaacgtcgc gacgaatttc gccttcaccg
      241 cgtcgttgcc gggccatacg gtctcgagcc agttgaagcc cgcggcgccg tgccccgtgt
      301 aggtgtagcg gttgatctcc gcggagacgc cggcgagcac gccgttctcg aagtagtcct
      361 cccacttcag cttgtcggcg gagcggttcg ccttgatggt gttcagccgc gacttgatct
      421 ccgccgtgcg cgcctggcgc cgcgcgctcg tgtactcggc gcaacgctcc ttgagccacg
      481 ggttggcgct cgcgagcggc tgcgcgcaca cgggcggcgg ctggcccggc aggcgcgact
      541 gcatcaggtc ctgcgcgaac tggagcctgc ccttggggcc cacctcgaga atcagcagcg
      601 gaaagtacga gttcgcgtag ggcgcgaacg cgttggccga cgaatcgtcc gccgacacca
      661 tcaccggagc cttgcgcccc ggcacctcga tgaaatcccc gccgcccatg ctgcccgccg
      721 aatagacggc gaggcgcttc acctgcggct tcagctggta gacgtgatgc gtggtgcagc
      781 aattcgcccc gcccgtgttg ctcgaaaaat gcaccgtggg ctggccgttg cccgcgaggt
      841 cgcggccgat cgactggatg gtccacttgg acccgggatc ggtctccttg ggattcgact
      901 gccacgcagg cttgccgccc ttgccgaaga tgagcgcatg ctgctcgccg ccgcccttgg
      961 gacgcgccac gaccgcctcg aactcgttga agctcttcga gtccaccgcc tccaggccct
     1021 gcgactcgag ccatgccttc gaatcctggg cgaacgcggg aatcgaagcc ccggccagca
     1081 tccagaacgc gatcgcgatc cagcgcatgg ggtccccctt ccgatgatga ttcccgcgta
     1141 aattaccacg ccgcgcggtt tacctgatcg cctccactcg gtcatagagc tcccgcggcc
     1201 acggcgcgtc gggcgaggtc accgccttct gcaccgcctg catgaagggc cggcgatcta
     1261 ccttcaccac ggtcttgccg cgtttctcga actccgtgac gagccggttc tcggcgtcgt
     1321 agatctcctg ggtggcgcgc gatgaggcct cccacaggac ttccgagaag atcgcgcggt
     1381 cggcgtcgtt cagcttcgcc caggtgcccg cccccatgac cgtcaccagc gactcggtga
     1441 tgtggccggt gaggatgatg tacttctgca cctcgtagaa cttcttcgcg tcgatcgtgg
     1501 gcagcggatt ttcctgcgcg tcgacggtgc cgttctgcag cgcgaggtac acctccgcga
     1561 acgcgatcgg cgtaggattc gcgcccaccg cgcgcgggaa catcacgaag agcggcgcgt
     1621 cgggcacgcg cagcttcatg cccttcatgt cctcgggctt ggtcaccatc ttgttcgccg
     1681 tgaggtggcg cagcccgtag taggtgtatc ccaggatctt cgccccgccc gtcttctcct
     1741 cgtagcccct ggccagatcc ttgaagaccg gcccgttgcg gaatttctgc caatggtcgt
     1801 agtcgcggaa gatgaaaggc cccgccgcga tcgagatcgg cggataggcg cgtcccgcga
     1861 aggcggtgcc cgtgtagatg atgtcgaccg tgccgagcgg caat
//
LOCUS       DASVII010000253         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_587545,
            whole genome shotgun sequence.
ACCESSION   DASVII010000253 DASVII010000000
VERSION     DASVII010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587545"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VF309_03250"
     CDS             complement(<1..1056)
                     /locus_tag="VF309_03250"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7465632.1"
                     /translation="MPRGHRGALATFLKFTVLVLAVVAASVSFAAEKIVSSRVWPAQE
                     YTRVTLESARPIRHQFFFVNNPERLVVDLEGVELDDELKGLPAKVGANDPYIQSVRVG
                     VNRPNVVRVVFDLKTEVKPSVFPLAPAGEYKNRLVLDIYPAKPADPLLALFAPKTDPI
                     GEIASAPMSQATAEAPALTLAALSPSAPAPAAPAAALPPTTLPTVKLDPNKPREPATR
                     LAARPKIDRLVIIAIDAGHGGEDSGARGRHGTREKDVTLAIARLLRSRIDQEPNMRAV
                     LVRDGDYFIPLMQRVSKSRRIQADLFVSIHADAWVRPDARGSSVFALSERGATSTAAR
                     MLAQRENESDLIGGVNLG"
     gene            complement(1017..1493)
                     /gene="tsaE"
                     /locus_tag="VF309_03255"
     CDS             complement(1017..1493)
                     /gene="tsaE"
                     /locus_tag="VF309_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7465633.1"
                     /translation="MVSLKTTLPDEQATLALGGALAQSLEPGLVIYLRGELGAGKTTL
                     ARGTLRALGFEGAVKSPTYALVEVYELSRLHLHHFDFYRFHDPREWTDAGFRESFNGR
                     TVSLIEWPEKAAGQLPPADLEIVLGLLEAGRSAELISNSPAGEKCLAGIEARWRRS"
     gene            1500..>2117
                     /gene="queG"
                     /locus_tag="VF309_03260"
     CDS             1500..>2117
                     /gene="queG"
                     /locus_tag="VF309_03260"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX7465634.1"
                     /translation="MQTAVQEAGVAARVKGWGRELGFDAIAIAGTDLAEEEARLLEWL
                     GRGWHGEMDYMARHGARRARPAELVPGTVSVITARLDYTPANARDSWEVLGDPASAFV
                     SRYALGRDYHKVLRAKLQALADRMTGEMGPFQYRVFTDSAPVMEVSLAARSGLGWRGK
                     HTLLLSRDAGSLFFLGELFVGLDLGKDAPVSAHCGTCERCIEICPT"
BASE COUNT          333 a          694 c          751 g          339 t
ORIGIN      
        1 gccgaggttc acgccgccga tgaggtcgga ctcgttctcg cgttgcgcga gcatgcgcgc
       61 cgcggtggag gtcgcgcccc gctcggacaa cgcgaacacg gaagagccgc gcgcgtcggg
      121 cctcacccat gcatccgcgt ggatcgagac gaagagatcg gcctggatgc gccgcgactt
      181 gctcacgcgc tgcatgagcg gaatgaagta gtcgccgtcg cgcaccagca ccgcgcgcat
      241 gttcggctcc tggtcgattc gcgacctgag gaggcgcgcg atcgcgagcg tcacgtcctt
      301 ctcgcgggtg ccgtggcggc cccgcgcgcc ggaatcctcg ccaccgtgtc ccgcgtcgat
      361 cgcgatgatg acgaggcgat cgatcttggg cctcgcggcg agccgcgtcg cgggctcgcg
      421 gggcttgttc ggatcgagct tgactgttgg aagcgtggtg ggcggaagcg cagccgcagg
      481 cgctgccggc gcaggtgccg agggtgagag cgcggcaagc gtgagcgcag gcgcctcggc
      541 ggtcgcctgc gacatcggcg cgctcgcgat ctcgccgatg gggtcggtct tcggcgcgaa
      601 cagcgcgagc agcgggtccg ccggcttggc cggatagatg tcgagcacca gccgattctt
      661 gtattcgccg gcgggcgcca gcggaaacac cgagggcttc acctccgtct tcaggtcgaa
      721 gaccacgcgg accacgttgg gacggttcac gcccacgcgc accgactgga tgtaggggtc
      781 gttcgcgccg accttcgccg gcaagccctt cagctcgtcg tcgagctcca cgccttcgag
      841 gtcgaccacg aggcgctccg ggttgttcac gaagaagaac tggtggcgta tcggccgcgc
      901 ggattcgagc gtcacgcggg tgtactcctg cgcgggccag acgcgcgagg acacgatctt
      961 ctccgcggcg aacgagacgc tcgcggcaac caccgcgagc accagcaccg tgaatttcag
     1021 gaacgtcgcc agcgcgcctc gatgcccgcg aggcatttct cccccgccgg ggagttcgaa
     1081 atgagttcgg cactgcgtcc cgcttcgagc aatcccagga cgatttccag atccgcgggc
     1141 ggcagttggc cagcggcctt ttccggccat tcgatcaggc tcacagtgcg cccgttgaag
     1201 ctctcccgaa agccggcgtc ggtccattcc ctcgggtcgt ggaacctata aaaatcaaag
     1261 tgatgcaagt gtaacctcga aagctcataa acttcaacaa gggcataggt cgggcttttg
     1321 acggccccct cgaagcccag ggcgcgaagc gtcccgcgcg cgagcgtggt cttgccggcg
     1381 ccgagctcgc cgcgcaggta gatcacgagg ccgggctcga gcgactgcgc gagcgcgccg
     1441 cctagtgcaa gtgtcgcctg ctcgtcggga agggtggttt tcagcgatac catcgggaaa
     1501 tgcaaacggc agttcaggaa gcgggcgtcg cggcacgcgt gaagggctgg ggacgcgagc
     1561 ttggattcga tgcgatcgcg atcgcgggga ccgacctcgc cgaggaagaa gcgcggcttc
     1621 tcgagtggct cggccgcggt tggcacggcg agatggatta tatggcacgc cacggcgcgc
     1681 gccgcgcgcg gcccgcggag ctcgtcccgg gaacggtgtc ggtgatcacg gcgcggctcg
     1741 actacacgcc ggcgaacgcg cgcgattcgt gggaggtgct cggcgatccg gcgagcgcgt
     1801 tcgtctcgcg ctacgcgctc gggcgcgact atcacaaggt cctgcgcgcc aagctgcagg
     1861 cgctcgccga ccggatgacg ggcgagatgg gcccctttca atatcgggtg ttcaccgaca
     1921 gcgcgcccgt gatggaggtg tccctcgcgg cgaggagcgg cctcggctgg cgcggcaagc
     1981 acacgctgct gctgtcgcgc gacgcgggct cgctcttctt cctgggcgag ctcttcgtgg
     2041 gcctcgacct cgggaaggac gcgccggtct ccgcccattg cggcacgtgc gagcgctgca
     2101 tcgagatctg ccccacg
//
LOCUS       DASVII010000254         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_336033,
            whole genome shotgun sequence.
ACCESSION   DASVII010000254 DASVII010000000
VERSION     DASVII010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336033"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF309_03265"
     CDS             <1..186
                     /locus_tag="VF309_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465635.1"
                     /translation="EKLGEAISLATPPVRDPGGTEQWIERSRAMLDCIVAGIQQIDDD
                     AKGARTDKRSAGSVDLF"
     gene            202..564
                     /locus_tag="VF309_03270"
     CDS             202..564
                     /locus_tag="VF309_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465636.1"
                     /translation="MKTILAVDDSATMREMVAFVLESAGYRVIEAEDGAQGLDRATST
                     SCDLVITDQNMPNMDGLTLVRSLRELRDYKSVPILLLTPESSDSMKAQGRAAGATGWL
                     VKPFDPATLLEVVQKVLR"
     gene            576..2366
                     /locus_tag="VF309_03275"
     CDS             576..2366
                     /locus_tag="VF309_03275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX7465637.1"
                     /translation="MAIDLNRFIATFFDEAQEHLESIEEKAMALGSGAADAETLNSIF
                     RAAHSIKGGSGTFGFTQLSDATHEMETLFDGLRKGKGEADEGTVRVLLDACDAFKAHL
                     ARLKAGERGFDPAMETIRIQLAGFRGADAAQPAAGPGAPAEESFFDAPAASAAPAKGE
                     AYGLFEEPAGEAPASPAAAEKYGLFETDGSLEQNRRKGDQSSIRVSVEKIDRIVNLVG
                     ELVIAQAMMQQSVATLEAKQDERLTHSLATLDRNTRDLQQAVMSIRMMPMEFVFSRFP
                     RLVYDLSSRLAKKVVLRTQGHETELDKELIELLVDPLTHVVRNAIDHGIESPEERRKA
                     GKPESGTVGMRATHRGGSVIIEVTDDGRGLDRERILAKARELGMAIDDSWTDAQAWGL
                     IFEAGFSTAKEVTNLSGRGVGMDVVRRNITSLGGSVSVTSRKGEGTTIAIQVPLTLAV
                     LDGMIVGVGDEQYIVPLEFVAEAFKPAEGDIKTVVNQASLVAVRGEHLPIVRLEDIVQ
                     LPAAGRDAGRRPDPLCLVVEVDQRRTALLVDSLIGQQQLVVKSLDTNLHPVPGLAGAT
                     ILGDGRVALILDVSAFTRATSARAMARVAA"
     gene            2363..>2671
                     /locus_tag="VF309_03280"
     CDS             2363..>2671
                     /locus_tag="VF309_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465638.1"
                     /translation="MMATTNETPTAASREFRFSAEHFRLISERVYEFSGIRLPEAKRE
                     MVYARLARRLRALGIGSFDDYVRFLELEPAEWEHCTNALTTNVTAFYREEHHFTVLAE
                     L"
BASE COUNT          456 a          926 c          909 g          380 t
ORIGIN      
        1 gagaagctcg gcgaggcgat ctcgctcgcc actccgccgg tgagggaccc gggggggacc
       61 gagcaatgga tcgaacgctc gcgcgcgatg ctcgactgca tcgtcgcggg catccagcag
      121 atcgacgacg acgccaaggg cgcgcgcacc gacaagcgca gcgccggcag cgtcgacctt
      181 ttctagaaga cgggcggcaa aatgaagacg atcctggcag tggacgattc cgcgacgatg
      241 cgggagatgg tcgcgttcgt cttggagagc gccggctacc gcgtgatcga ggccgaggac
      301 ggcgcgcagg ggctcgaccg ggccaccagc acctcgtgcg acctggtgat caccgaccag
      361 aacatgccga acatggacgg gctcacgctc gtgaggagcc tgcgcgagct gcgcgactac
      421 aagagcgtgc cgatcctgct gctcaccccc gaatcctccg acagcatgaa agcccagggc
      481 cgcgccgcgg gggcgaccgg ctggctggtg aagcccttcg accccgcgac ccttctcgag
      541 gtcgtgcaga aagtcctgcg ctagaagcgc gcgccatggc gatcgacctg aatcgtttca
      601 tcgcgacgtt cttcgacgag gcccaggagc acctggagtc catcgaggag aaggcaatgg
      661 cgctcggctc gggagccgcg gacgcggaga ccctcaactc gattttccgc gcggcccatt
      721 cgatcaaggg cggcagcggc accttcggct tcacgcagct ttccgacgcg acccacgaga
      781 tggagacgct cttcgacggc ctgcgcaagg gcaagggcga ggccgacgaa ggcaccgtgc
      841 gcgtgctcct ggacgcctgc gacgccttca aggcgcacct cgcgcggctg aaggccggcg
      901 agcgcggctt cgacccggcc atggagacca ttcgcatcca gctcgccggg tttcgcggcg
      961 cggacgccgc gcagcccgcc gcgggcccgg gcgcgcccgc cgaggaatcc ttcttcgatg
     1021 cgccggccgc gagcgcggcc cctgcgaagg gcgaggccta cggcctgttc gaggagccgg
     1081 cgggcgaagc gcccgcgagc ccggccgcgg ccgagaagta cggcctgttc gagaccgacg
     1141 gcagcctcga gcagaacaga cgcaagggcg atcagtcctc gatacgcgtg agcgtcgaga
     1201 agatcgatcg catcgttaac ctggtcggcg agctcgtgat cgcgcaggcg atgatgcagc
     1261 agtcggtggc gacgctcgag gccaagcagg atgagcgcct cacccacagc ctcgcgacgc
     1321 tcgaccgcaa cacgcgcgac ctgcagcagg cggtgatgtc gatccgcatg atgccgatgg
     1381 agttcgtctt cagccgcttc ccgcggctcg tctacgacct ctccagccgg ctggcgaaga
     1441 aagtggtgct gcgcacccag ggccacgaga ccgagctcga caaggaattg atcgagcttc
     1501 tcgtcgatcc gctcacccac gtggtgcgca acgcgatcga ccatggcatc gagtcgcccg
     1561 aggagcgccg caaggcgggc aagcccgagt cggggaccgt cggcatgcgg gcgacgcatc
     1621 gcggcggcag cgtgatcatc gaggtgaccg acgacggccg cggcctcgat cgcgagcgca
     1681 tcctcgccaa ggcgcgcgag ctcggcatgg cgatcgacga ctcgtggacc gacgcgcagg
     1741 cctggggcct catcttcgag gcgggcttct ccacggcgaa ggaggtcacc aatctctccg
     1801 ggcgcggcgt cggcatggac gtcgtgcgcc gcaacatcac ctccctcggc ggcagcgtct
     1861 cggtgacgtc caggaagggc gagggcacca cgatcgcgat ccaggtgccc ctcacgctcg
     1921 cggtcctcga cggcatgatc gtcggcgtgg gcgacgagca gtacatcgtg cctctcgaat
     1981 tcgtggcgga agcgttcaag cccgccgaag gcgacatcaa gaccgtcgtg aaccaggcct
     2041 cgctggtcgc ggtgcgcggc gagcacctgc cgatcgtgcg gctcgaggac atcgtccagc
     2101 tgcccgcggc cgggcgcgac gccgggcggc gccccgatcc cctgtgcctc gtcgtcgagg
     2161 tggatcaacg ccgcaccgcg ctcctggtcg acagcctcat cggccagcag cagctcgtgg
     2221 tgaagtccct cgacacgaac cttcatccgg tgccgggcct cgcgggggcg accattctcg
     2281 gcgacgggcg cgtcgccctc atcctcgacg tgagcgcgtt cacgcgcgcc acgtcggcgc
     2341 gggcgatggc gcgagtggcg gcatgatggc cacgacgaac gagaccccga ccgccgcgag
     2401 ccgggagttc cgcttcagcg cggagcactt tcgcctgatc tccgagcgcg tctacgagtt
     2461 ctctggcatt cgccttcccg aagcgaagcg cgaaatggtc tatgcgcgcc ttgcgcggcg
     2521 cctgcgcgcc ctgggcatcg gctcttttga cgactacgtt cgcttcctcg agctcgagcc
     2581 cgccgaatgg gagcattgca ccaacgcgct caccaccaac gtgaccgcct tctaccgcga
     2641 ggagcaccac ttcaccgtgc tcgccgagct c
//
LOCUS       DASVII010000255         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_155840,
            whole genome shotgun sequence.
ACCESSION   DASVII010000255 DASVII010000000
VERSION     DASVII010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155840"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF309_03285"
     CDS             complement(<1..231)
                     /locus_tag="VF309_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7465639.1"
                     /translation="MLIFRQLADPPSSTYTYLLGDRGSREALLIDPVFEQALRDAALV
                     EELGLKLKMTLDTHTHADHVTGVIFSLRPSSST"
     gene            296..751
                     /locus_tag="VF309_03290"
     CDS             296..751
                     /locus_tag="VF309_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7465640.1"
                     /translation="MATIRRIGARDERRWRELFAQYTRFYEREPDEAITRHVWSRIMD
                     PAAPVYAIVAEDAAAGVVGIANYLLHESTGSLAPVCYLQDLFVDPAMRGGGVGKALID
                     WLVAETAAQGWSRLYWTTRENNYRARALYDKYTPHSGFLRYALSPKKTS"
     gene            complement(771..>1834)
                     /locus_tag="VF309_03295"
     CDS             complement(771..>1834)
                     /locus_tag="VF309_03295"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7465641.1"
                     /translation="FSVIPRPADLPIARMKEAEAIAALSKRADDAARADLFSGAVLVA
                     RDGKVLLERAWGFADRVRLAMASPSTKFRLGSMNKMFTAVAILQLVEAGKVALTDPVA
                     KYLPDYPNRELAAKVTVRDLLTHTAGMGDIFGPEFFQKRLTLKEHADYVKLYGAREQE
                     AGAGTFHYSNYGYILLGSIIEKASAMSYYDYVQKNVFEPAGMASTGSLPENVPVVNRA
                     RGYMRRQGHWVSNAATLPWRGTAAGGGYSTVGDLYRFATALSSGKLLSKAMLAEATRA
                     QATPPVYGYGFFLQGEGTLQHYGHNGGAPGMNGELRIYPQLGYVIVSLSNVDPPGATR
                     MADFIEARMPIAAAKAERP"
BASE COUNT          291 a          615 c          628 g          300 t
ORIGIN      
        1 cgtcgaagag ctcggcctca agctgaagat gacgcccgtc acgtgatcgg cgtgggtgtg
       61 cgtgtcgagc gtcatcttca gcttgaggcc gagctcttcg acgagggcgg cgtcgcgcag
      121 cgcctgctcg aacacgggat cgatcagcaa cgcttcgcgg ctcccgcgat cgccgaggag
      181 gtacgtgtac gtcgacgacg gaggatcggc gagttgccgg aagatcagca cgcgggggtc
      241 tctttcgcga tggccaagcc tgatcgtaca ctccggattc gggaggagga cggccgtggc
      301 aacgatacga agaatcgggg cgcgcgacga gcggcgctgg cgcgagctct tcgcgcaata
      361 cacgcgtttc tacgagcgcg agccggacga ggcgatcacg cggcacgtgt ggtcgcggat
      421 catggatccc gcggcgcccg tctacgccat cgtcgcggag gacgcggccg cgggcgtggt
      481 cggcatcgcc aactacctcc tgcacgagag cacgggctcg ctcgcgcccg tctgctacct
      541 gcaggatctc ttcgtcgatc ccgcgatgcg cggcggcggc gtcggcaagg cgctcatcga
      601 ttggctcgtg gcggagaccg cggcgcaggg gtggtcgcgc ctgtactgga ccacgaggga
      661 gaacaactac cgggcgcggg cgctgtacga caaatacacg ccccacagcg gtttccttcg
      721 ctatgcgctg tcgcccaaga agacgagctg agacgccgcg cgcccggcgc tcagggccgc
      781 tcggccttcg ccgccgcgat gggcatcctc gcctcgatga agtccgccat gcgcgtggcg
      841 cccgggggat cgacgttgct cagcgacacg atcacgtagc cgagctgggg gtagatgcgc
      901 agctcgccgt tcattcccgg cgcgccgccg ttgtggccgt agtgctggag cgtgccttcg
      961 ccttgaagga agaagccgta tccgtacacc ggcggcgtcg cctgggcgcg cgtcgcctcg
     1021 gcgagcatcg ccttcgagag gagcttgccc gacgagaggg ccgtcgcgaa ccggtagaga
     1081 tcgccgacgg tggaatagcc gccgccggcc gccgttcccc tccacggcag cgttgccgcg
     1141 ttcgacaccc aatgcccctg tcggcgcatg tagccgcgcg cgcggttcac gacgggcacg
     1201 ttctcgggaa gcgagcccgt cgaggccatg cccgcgggct cgaacacgtt cttctgcacg
     1261 tagtcgtagt acgacatcgc gctcgccttc tcgatgatcg acccgagaag gatgtagccg
     1321 tagttcgagt agtggaaggt gccggcgccc gcttcctgct cgcgcgcgcc gtagagcttc
     1381 acgtagtccg cgtgctcctt cagcgtcagg cgcttctgga agaattccgg cccgaagatg
     1441 tcgcccatcc ctgcggtgtg cgtcagcagg tcgcgcacgg tgaccttcgc cgcgagctcc
     1501 cggttgggat agtcgggaag atatttcgcg accgggtcgg tcagcgccac cttgcccgcc
     1561 tcgacgagct gcaggatcgc caccgccgtg aacatcttgt tcatcgagcc caggcggaat
     1621 ttcgtgctcg ggctcgccat cgcgagcctc acgcgatccg cgaagcccca cgcgcgctcg
     1681 agcagcacct ttccgtcgcg cgccacgaga accgcgcccg agaagagatc ggccctggcg
     1741 gcatcgtcgg cgcgcttcga caacgcggcg atcgcctccg cctccttcat gcgcgcgatg
     1801 ggaagatccg cggggcgcgg gatcacgctg aaag
//
LOCUS       DASVII010000256         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_515788,
            whole genome shotgun sequence.
ACCESSION   DASVII010000256 DASVII010000000
VERSION     DASVII010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515788"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1080
                     /locus_tag="VF309_03300"
     CDS             82..1080
                     /locus_tag="VF309_03300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7465642.1"
                     /translation="MQTPSLMRLPEVSVVIPVYNEEQCLAALFTRLYPALDDMQRATG
                     LDYEVVFVNDGSRDRSAALLREQFQKRPEVTRVILFNGNFGQHMAILAGFEHVRGGRI
                     VTLDADLQNPPEEIAKLLAKMDEGHDYVGSIRRMRQDAAWRRWASKAMNRMRERITHI
                     KMTDQGCMLRAYDRPIIDAIRASREVSTYIPALAYSFSQNPTEIEVEHEERQAGESKY
                     SLYKLIRLNFDIVTGFSVMPLQLFSLFGIFISLVSLGTYVVVIIQRIVSGTADGLWVI
                     WDRDILQFFLTGMVLFGLGLIGEYVGRIYQQVRERPRFLIQAILERPAPEEGKNGL"
     gene            1070..>1696
                     /locus_tag="VF309_03305"
     CDS             1070..>1696
                     /locus_tag="VF309_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX7465643.1"
                     /translation="MASDTSFSGVEGPYTAVVFAYHDVGVRCLSVMLAHGVRIPLVVT
                     HRDDPEENVWFESVEKLARDHDLPVATPDDPNAPRFLQRLADISPDFIFSFYYRFMLS
                     QAVLATAMRGAFNMHGSLLPKFRGRAPVNWAVLRGETSTGATLHEMVEKPDAGRIVDQ
                     FPVPILPDDRAIDVFRKVTVAAELLLDRALPGVLDGMAPLITQDLAQGG"
BASE COUNT          266 a          590 c          539 g          301 t
ORIGIN      
        1 gcgcagcatc ctcacgctgc cgctttatcc cgccctgcgt tcgggcgatg tcgagcgggt
       61 ctgtcttggt ctggccgaag catgcaaacg ccttctctga tgcgcctccc cgaagtctcg
      121 gtcgtgatcc ccgtgtacaa cgaggaacag tgccttgcgg cgctgttcac gcgcctgtac
      181 ccggcgctgg acgatatgca gcgcgccacc gggctggact acgaagtggt gttcgtgaac
      241 gacggcagcc gcgaccgctc ggcggcgctg ctgcgcgagc agttccagaa gcgccccgag
      301 gtgacgcgcg tcatcctctt caacggcaac ttcggccagc acatggcgat cctcgccggc
      361 ttcgagcacg ttcgcggcgg ccgcatcgtg acgctggacg cggacctgca gaacccgccc
      421 gaggagatcg cgaagctcct cgccaagatg gacgagggtc acgactacgt gggctcgatc
      481 cggcgcatgc gccaggacgc cgcctggcgg cgctgggctt cgaaggcgat gaaccgcatg
      541 cgcgagcgca tcacgcacat caagatgacc gaccaggggt gcatgctgcg cgcctacgac
      601 cggcccatca tcgacgcgat ccgcgcctcg cgagaggtga gcacctatat ccccgcgctc
      661 gcgtattcct tctcgcagaa tcccacggag atcgaggtcg agcacgagga gcgccaggcg
      721 ggcgaatcga agtactcgct ctacaagctg atccgcctga acttcgacat cgtcaccggc
      781 ttctcggtga tgccgctgca gctcttctcg ctcttcggca tcttcatctc gctcgtctcg
      841 ctgggcacct acgtcgtggt catcatccag cgcatcgtgt cgggcaccgc ggacggcctg
      901 tgggtgatct gggaccggga catcctgcag ttcttcctca ccggcatggt gctcttcggg
      961 ctgggcctaa tcggcgagta cgtggggcgc atctaccagc aggtgcgcga gcggcctcgc
     1021 ttcctcatcc aggcgatcct ggaaaggcct gcgccggaag aggggaaaaa tggcctctga
     1081 caccagtttc tccggcgtgg aggggcccta cacggcggtc gtgttcgcct atcacgacgt
     1141 gggcgtgcgc tgcctgtcgg tgatgcttgc ccatggcgtg cgcattcccc tggtcgtcac
     1201 gcaccgcgac gatcccgagg agaacgtctg gttcgagagc gtggagaagc tcgcccgcga
     1261 ccacgatctc ccggtggcga cgcccgacga cccgaacgcg ccgcgattcc tccagcgcct
     1321 tgccgacatc tcgcccgatt tcatcttctc gttctactac cggttcatgc tctcgcaggc
     1381 ggtgctcgcc acggcgatgc gcggcgcgtt caacatgcac ggctccctcc tgccgaagtt
     1441 ccgcggccgc gctccggtca actgggcggt gctgcgcggc gagacctcga ccggcgcgac
     1501 gctgcacgag atggtcgaga agcccgacgc cgggcgaatc gtcgaccagt tcccggtgcc
     1561 gatccttccc gacgaccgcg cgatcgacgt cttccgcaag gtgaccgtgg ccgcggagct
     1621 gctcctcgat cgcgcgctgc ccggagtcct cgacggcatg gcgccgctca tcacccagga
     1681 cctcgcgcag ggcggg
//
LOCUS       DASVII010000257         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_336255,
            whole genome shotgun sequence.
ACCESSION   DASVII010000257 DASVII010000000
VERSION     DASVII010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336255"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VF309_03310"
     CDS             complement(<1..575)
                     /locus_tag="VF309_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7465644.1"
                     /translation="MKGSVLETESLRILDDALGALASGFASLPAAAAHGDAEAMARVL
                     HEVAERLQDNYPYFHPLYAGQMLKPPHPMARVAGALAAWINPNNHALDGGRASSQMEK
                     EAVAAIARMFGWDTHLGHLCSGGTMANLEALWIARELRPGTKVVASQQAHYTHRRLSA
                     VLAIDFESVACDGAGRMDIAALERILAAGNVG"
     gene            complement(661..939)
                     /gene="rpmA"
                     /locus_tag="VF309_03315"
     CDS             complement(661..939)
                     /gene="rpmA"
                     /locus_tag="VF309_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7465645.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGLKAYGGELVNAGSILVRQRGTT
                     FHPGAHVGIGKDHTLFAMKTGKVKYSEHGPKSRSTVSIEPASLQPKQA"
     gene            complement(962..1270)
                     /gene="rplU"
                     /locus_tag="VF309_03320"
     CDS             complement(962..1270)
                     /gene="rplU"
                     /locus_tag="VF309_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7465646.1"
                     /translation="MYAVIKTGGKQYRVAPGEKIKIEQIPADVGAQIVLDQVLMVADG
                     DAVKLGNPLVSGATVSATVIAHGRGVKIKIFKLRRRKHYQKTQGHRQNYTEIRVDAIA
                     "
     gene            1400..>1622
                     /locus_tag="VF309_03325"
     CDS             1400..>1622
                     /locus_tag="VF309_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEX7465647.1"
                     /translation="MSLEVIRAPVADDLKHVDEVIRRRLDSEVVLIRTIASYIVASGG
                     KRLRPALVLLASKAFGREAGARHGLAAVVE"
BASE COUNT          252 a          541 c          525 g          304 t
ORIGIN      
        1 cccacgttgc ccgcggcgag aatccgctcg agcgcggcga tgtccatgcg ccccgcgccg
       61 tcgcaggcga cggattcgaa atcgatcgcc aggaccgcgc tcaggcgccg atgcgtgtag
      121 tgcgcctgct gcgaggcgac gaccttcgtg ccgggacgca gctcgcgcgc gatccacaac
      181 gcctcgaggt tcgccatcgt cccgccgctg cacaggtggc ccaggtgggt gtcccagccg
      241 aacatgcgcg cgatcgcggc gacggcttcc ttctccattt gcgagctcgc gcgccccccg
      301 tcgagcgcgt ggttgttggg attgatccac gcggcgagcg cgcccgcgac gcgcgccatc
      361 ggatgcggcg gcttcagcat ctggcccgcg tagagcggat ggaagtaggg atagttgtcc
      421 tggaggcgct cggcgacttc gtgaagcacg cgcgccatcg cctcggcatc gccgtgcgcc
      481 gcggcggccg gcagggaagc gaagccgctc gcgagcgcgc ccagcgcgtc gtcgagaatg
      541 cgcagcgact ccgtttccag caccgagccc ttcatcggcc gcctcccacg cgcgtctgtt
      601 tcggaaaaga aaaaagcccc gtgcgaagac ggggctcttt cgtgcagcgg aaaagagccg
      661 tcaggcctgc ttcggttgga gcgaagcagg ctcgatcgac acggtcgacc tgctcttcgg
      721 gccgtgctcg gagtacttca ccttgcccgt cttcatcgcg aagagcgtgt gatccttgcc
      781 gatgcccacg tgggcgcccg ggtggaacgt cgtgccgcgc tggcgaacga ggatcgagcc
      841 cgcgttcacc agctcgccgc cgtatgcctt gaggccgagg cgcttcgact cggaatcgcg
      901 gccgttgcgg gaagacccgc ctgctttctt gtgtgccatt gctgtgtcct tgaactcgag
      961 ctcaggcgat cgcgtccaca cggatttccg tgtagttctg gcgatggccc tgcgtcttct
     1021 ggtagtgctt gcggcgacgc agcttgaaga tctttatctt cacgccgcgc ccgtgagcga
     1081 tgacggtggc ggagactgtc gctccggaca ccaggggatt accgagcttc acggcgtcgc
     1141 cgtccgcgac catcaagacc tgatcgagga caatctgagc gccaacgtct gccggtatct
     1201 gttctatttt gattttttcg ccgggggcaa cgcggtactg cttgccgccg gtttttatga
     1261 ccgcgtacat tgtggactcc attgcgtgga aacgtgtctc cctcagcctt ctatcgaccg
     1321 atggcagaga gaactcgcta taatacgcga aatctcccat aaggtcaaag acttgtccct
     1381 gcgtcatccg ctggctccgg tgagcctcga agtcatccgc gcacccgttg ccgacgacct
     1441 caagcacgtc gacgaagtca tccgccggcg cctcgactcc gaggtggtcc tcatccgcac
     1501 catcgcgagc tacatcgtcg cgagcggcgg caagcggctg cgcccggccc tggtgctgct
     1561 cgcgtcgaag gccttcgggc gcgaggccgg cgcgcggcac gggctcgcgg cggtcgtcga
     1621 gt
//
LOCUS       DASVII010000258         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_300160,
            whole genome shotgun sequence.
ACCESSION   DASVII010000258 DASVII010000000
VERSION     DASVII010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300160"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF309_03330"
     CDS             <1..375
                     /locus_tag="VF309_03330"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465648.1"
                     /translation="NRYKFHFVAVTWHETLGRVLTVMSCEGDYPDGRMTFPLGAPIRL
                     PDSGAIGLAAEVNGATLQLFYDAGGGWRDAGPMLDASVISDEGGRGAHGSFTGAFIGM
                     LAFDTSGAACPANFHHFDYSPR"
     gene            complement(360..1403)
                     /locus_tag="VF309_03335"
     CDS             complement(360..1403)
                     /locus_tag="VF309_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7465649.1"
                     /translation="MRPFGWAVVGPGNIARRFAQAVQGLEGAALVAVQGRDPGRCAVF
                     ARKWARDGASMDVASDIASLACNERVDAIYVATPHAFHAAAIRECIAAGKPVLCEKPL
                     VAYHCEGLAVVALARRHRVFLMEAVWTRFLPIYDVVRAWLRGGRIGRVRAIQSSFCFN
                     SPFDPSSRNYDPAQAGGSLLDLGVYNLTMTRWTLEAALGRCPPLQSLQASGVVGPSGV
                     DHRASATLEFPGGIVSQLTCGFEMSADNTFRIAGELGSIVLPQFWQATDAELRVAGKD
                     AVAAHRPHRINGFEGEIEEAMAMIRRGDIESPRISHDETLATLEWMDRIREQIGVRYP
                     FETDSGVRAQRGE"
     gene            complement(1420..1680)
                     /locus_tag="VF309_03340"
     CDS             complement(1420..1680)
                     /locus_tag="VF309_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465650.1"
                     /translation="MKSNRLLFPVCMATLAALCGCAGPVIHAPDRGRFGDAVQKCVAS
                     AHDRNACARLANQRCGAGFEVFEMDAVEEDGVERRAFHFRCL"
     gene            complement(1714..>2261)
                     /locus_tag="VF309_03345"
     CDS             complement(1714..>2261)
                     /locus_tag="VF309_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7465651.1"
                     /translation="RHYGVSVEKVEEGLKAIEFPGVQSVQVVYNIFRQRPAERLFDEA
                     QRRGVGILARLPLSSGLLAGKLTSASTFASDDHRQFNREGAAFDRGETFSGLDYRTGL
                     DAVEALRPWVPPGQSMARMALRWIQMHPAVTCSIPGCRRPAHVADNMAAADLPPLPAE
                     AMAAIDEVYRSRVKNHVHQRW"
BASE COUNT          371 a          791 c          754 g          345 t
ORIGIN      
        1 aaccgctaca agttccattt cgtcgccgtc acctggcacg agacactggg acgcgtgctc
       61 accgtgatgt cgtgcgaggg cgactatccc gacggccgaa tgaccttccc gctgggcgcg
      121 ccgattcggc tcccggattc cggcgccatc ggtctcgccg ccgaagtgaa cggggcgacg
      181 ctgcagctct tctacgacgc gggaggcggc tggcgcgacg cgggcccgat gctggacgcc
      241 agcgtcatct ccgacgaagg ggggcgaggc gcccacggct cgttcaccgg cgcgttcatc
      301 ggcatgctcg ccttcgacac gtcgggcgcc gcgtgccccg ccaacttcca ccacttcgac
      361 tactcgccgc gctgagcgcg aacgccggaa tcggtttcga agggatagcg cacgccgatt
      421 tgctcgcgga tgcgatccat ccactcgagc gtcgccaggg tctcgtcatg cgagatgcgc
      481 gggctctcga tgtcgccgcg gcggatcatg gccatcgcct cctcgatctc gccttcgaag
      541 ccgttgatgc ggtgcggcct gtgcgcggcg acggcgtcct tgccggcgac gcgcagctcg
      601 gcatcggtcg cctgccagaa ttgcgggagg acgatgctgc cgagctcgcc ggcgatgcga
      661 aaggtgttgt ccgcgctcat ctcgaatccg caggtgagct gcgagacgat gcctccggga
      721 aactcgagcg tcgcgctcgc gcgatggtcg accccgctcg gcccgacaac gccggaagcc
      781 tgcagtgact gcagcggcgg gcagcggccc agggccgctt cgagcgtcca gcgagtcatc
      841 gtgaggttgt agaccccgag gtcgaggagc gacccgccgg cctgcgccgg atcgtagttg
      901 cggctcgagg ggtcgaaggg ggaattgaag cagaagctcg actggatcgc gcgaacgcgc
      961 ccgatgcggc cgcctcgcag ccacgcccgc accacgtcgt atatcggcag gaagcgcgtc
     1021 cataccgctt ccatgaggaa cacgcgatgc cggcgcgcca atgccacgac ggcgaggcct
     1081 tcgcagtggt aggccacgag cggcttctcg caaaggacgg gcttcccggc ggcgatgcat
     1141 tcgcgaatcg cggccgcgtg gaaggcatgc ggcgtggcca cgtagatcgc gtcgacgcgc
     1201 tcgttgcacg cgagcgaagc gatatcgctc gccacgtcca tcgaggcgcc atcgcgcgcc
     1261 catttgcgcg cgaacacggc gcagcgcccc gggtcgcggc cctgaaccgc gacgagggcc
     1321 gcgccctcca agccctgcac ggcctgcgcg aagcgtcggg cgatgtttcc gggaccgacg
     1381 acggcccagc cgaacggtct catgtaaccc ctccggaatt cacaagcacc tgaagtggaa
     1441 ggcgcgccgt tcgacgccat cctcctcgac ggcgtccatc tcgaacacct cgaatccggc
     1501 gccgcagcgc tggttcgcga ggcgcgcgca ggcgttgcga tcgtgcgcgc tggcaacgca
     1561 cttctgcacc gcgtcgccga agcggccgcg atcgggcgca tgaatgacag ggcccgcgca
     1621 gccgcaaagc gccgcgagcg tcgccatgca cacagggaag agcaggcggt tcgatttcat
     1681 ggaattctcc ttcggtatcg tcccgagaat ttcctaccag cgctggtgca cgtggttctt
     1741 cacgcggctg cggtacacct cgtcgatcgc cgccatcgcc tccgccggca atggcggcaa
     1801 atcggccgcg gccatgttgt cggcgacgtg cgcgggacgc ctgcagcccg gaatgctgca
     1861 ggtgacggcc gggtgcatct ggatccagcg caacgccatc cgcgccatgc tttgccccgg
     1921 cggcacccag gggcgaagag cctcgaccgc atcgaggccc gtccgatagt ccaggcccga
     1981 gaaggtctcg ccccgatcga aggcggcacc ctcgcgattg aactggcggt gatcgtcgga
     2041 cgcgaaggtg ctcgcgctcg tgagcttgcc cgcgagcaat cccgaagaaa gcggcaagcg
     2101 cgcgagaatg cccacgccgc gccgttgcgc ctcgtcgaag agccgctccg cgggccgctg
     2161 gcgaaagatg ttgtagacga cctgcaccga ctgcactccc gggaactcga tcgccttcag
     2221 gccctcctcc accttctcga cgctcacgcc gtagtgccga a
//
LOCUS       DASVII010000259         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_300292,
            whole genome shotgun sequence.
ACCESSION   DASVII010000259 DASVII010000000
VERSION     DASVII010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300292"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF309_03350"
     CDS             complement(<1..414)
                     /locus_tag="VF309_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465652.1"
                     /translation="MSEARARIAAMFLFLGSLALASCASEPTSKDAFTFAVMGDTPYS
                     EREEAPFLAAIERMNGEPLAFVVHVGDIKAGSNSRCSDELFEKRKAQFNLSKHPFIYT
                     PGDNEWTDCRRASNGGADPLERLAKLREVFFSDGRS"
     gene            complement(407..2974)
                     /gene="mutS"
                     /locus_tag="VF309_03355"
     CDS             complement(407..2974)
                     /gene="mutS"
                     /locus_tag="VF309_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7465653.1"
                     /translation="MKLEAPGSAHTPMMQQYLALKAEYPDMLLFYRMGDFYEMFHADA
                     ERAAKLLDITLTTRGQSAGQPIRMAGVPYHSVEHYLAKLVKAGESVAICEQVGDPATA
                     KGPVERRVMRVVTPGTLTDSALLPASRESLLVAAHAAGDVAGIAWISLASGRLTVSEV
                     PRARLRAELERLQPAELLASHDLAIELDPAPAMKRLDPWQFDAAAAARELSRQFGTAD
                     LSGYGCDGMDRAIAAAGALLAYCRHTQQSTLPHVTGLRVERDGEFVTMDAPTRRNLEI
                     TETLSGAESPTLFSLLDRCATSMGSRRLRHWLHHPLRDTSVLARRHAAIAALAHPSSR
                     LRHDRLAQLFAHWSDVERITARVALRTARPRDLAGLRDTLATLPRLHAALAESGEAGL
                     EGAAHELRPASDLHAALAASLMDEPAAVIREGGVIRDGRDPLLDELRAIQKDCGAFLL
                     DMEKRERERTGIANLRVEFNRVHGFFIEVTQAQLDKVPIEYKRRQTMKNAERFITPEL
                     KAFEDKALAAGERALALEKSLYEALLDELNSHIARLQSIASAAAELDALCALAAGAET
                     LGLSRPEFTREDRIDILEGRHLVVESQVDDFIPNDTSFTRSRQLLLVTGPNMGGKSTY
                     MRQVAQIALLAYCGSFVPAKSATLGPVDRIFTRIGASDDLAGGRSTFMVEMTEAAAIL
                     NNATPTSLVLVDEIGRGTSTFDGLALAYAIARHLAEEVRCYTLFATHYFELTQLAAQL
                     PNVANVHLDAVEHKDRIVFLHRLEAGPADRSYGIHVAHLAGIPKDVVRAAKKHLGELE
                     AQLRPAGAQPDLFAQPRAAEASAVSPALAALESVKPDELSPKEALETLYRLRKLLDE"
     gene            complement(3075..>3386)
                     /locus_tag="VF309_03360"
     CDS             complement(3075..>3386)
                     /locus_tag="VF309_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7465654.1"
                     /translation="QTAHQQASHLVRTIERRLAGAAPRPYRYRDFGSLVSLGGYDTIG
                     QLMGFISSEKFRVEGWLAKLFYLSLYRQHIWALFGFWRMALDTLARWIRRQTEPKVKL
                     H"
BASE COUNT          500 a         1108 c         1241 g          537 t
ORIGIN      
        1 cgaccggcca tcggagaaga acacttcgcg cagcttcgcg agtcgctcga ggggatcggc
       61 gccgccgttg gaggcgcggc ggcagtcggt ccactcgttg tcgcccggcg tgtagatgaa
      121 cggatgcttc gagaggttga actgggcctt gcgcttctcg aagagctcgt cgctgcatcg
      181 cgagttgctg ccggccttga tgtcgcccac gtgcaccacg aatgcgagag gctccccgtt
      241 catgcgctcg atcgccgcga ggaagggcgc ctcctcgcgc tcgctgtagg gcgtgtcgcc
      301 catcacggcg aaagtgaatg cgtccttcga ggtgggctcg ctcgcgcagc tcgccagcgc
      361 gagcgagccc aggaaaagga acatcgcggc gatccgggcg cgcgcttcac tcatcgagga
      421 gcttcctcaa gcggtagagc gtctcgagcg cttccttggg cgagagctcg tcgggcttca
      481 cgctttcgag cgccgccagg gcgggtgaaa cggccgatgc ctcggccgcg cggggttggg
      541 cgaagagatc gggctgcgcg ccggcgggac gcagctgcgc ctccagctct cccaggtgct
      601 tcttcgccgc gcgcacgaca tccttcggga tgcccgcgag gtgcgcgacg tggattccgt
      661 aggagcgatc cgcgggaccg gcctccagcc ggtgcaggaa cacgatgcga tccttgtgct
      721 ccaccgcgtc gaggtgcacg ttggcgacgt tcggcagctg cgccgcgagc tgcgtgagct
      781 cgaagtagtg cgtcgcgaac agcgtgtagc agcgcacctc ctcggcgagg tggcgcgcga
      841 tcgcgtaggc gagggcgagg ccgtcgaagg tggaggtgcc gcggccgatc tcgtcgacca
      901 gcacgaggct cgtcggcgtc gcgttgttca agatcgccgc ggcctcggtc atctcgacca
      961 tgaaggtcga gcgcccgccc gcgaggtcgt cggaggcccc gatgcgcgtg aagatgcggt
     1021 cgacggggcc cagcgtcgcg gacttcgcgg gaacgaagct tccgcaatac gcgagaagcg
     1081 cgatctgcgc gacctgccgc atgtaggtcg atttgccgcc catgttcggc cccgtgacga
     1141 ggaggagctg gcgcgagcgc gtgaagctcg tgtcgttcgg gatgaaatcg tcgacctggc
     1201 tttcgaccac gaggtggcgg ccctcgagga tgtcgatgcg gtcctcgcgc gtgaattcgg
     1261 gccgggagag gccgagggtc tcggcgcccg cggcgagcgc gcagagcgca tcgagctcgg
     1321 ccgcggcgga ggcgatcgac tgcagccgcg cgatgtggga attgagctcg tcgaggagcg
     1381 cctcgtacag gctcttctcg agcgcgaggg cccgctcgcc cgcggcgagc gccttgtcct
     1441 cgaaggcctt cagctccggc gtgatgaagc gctcggcgtt cttcatcgtc tggcggcgct
     1501 tgtactcgat tggcaccttg tcgagctgcg cttgcgtcac ctcgatgaag aaaccatgca
     1561 cgcggttgaa ttccacgcgc aggttggcga tgccggtgcg ctcgcgctcg cgcttctcca
     1621 tgtcgaggag gaacgcgccg caatccttct ggatcgcgcg cagctcgtcg agcaggggat
     1681 cgcggccgtc gcggatcacg ccgccttcgc ggatcacggc ggccggctcg tccatgaggc
     1741 tcgccgcgag ggccgcgtgc aggtcgctcg ccgggcgcag ctcgtgggcc gcgccttcga
     1801 gccccgcttc gccgctttcc gcaagcgcgg cgtgcagccg agggagcgtg gccagcgtgt
     1861 cgcgcaaccc ggcgaggtcg cgggggcgcg cggtgcgcag cgcgacgcgc gcggtgatgc
     1921 gctcgacgtc cgaccagtgc gcgaagagct gcgcgaggcg atcgtggcgc aggcgcgaag
     1981 acggatgcgc gagcgccgcg atagccgcgt ggcggcgcgc gagcaccgac gtgtcgcgca
     2041 gcgggtggtg cagccaatgg cgaagcctgc ggcttcccat cgacgtcgcg cagcgatcga
     2101 ggagcgagaa gagcgtgggc gattccgctc ccgacagggt ctcggtgatc tcgaggttgc
     2161 ggcgtgtggg cgcatccatc gtcacgaact cgccgtcgcg ttcgacgcgc agtcccgtca
     2221 cgtgaggcaa cgtcgattgc tgcgtgtggc ggcaataggc gaggagcgct cccgccgcgg
     2281 cgatcgcgcg atccatgccg tcgcagccgt agcccgaaag atccgcggtg ccgaactgcc
     2341 gcgagagctc gcgcgccgcg gcggcggcgt cgaactgcca cgggtcgagg cgcttcatcg
     2401 cgggcgccgg gtcgagctcg atcgcgaggt cgtggctcgc caggagctca gcgggctgca
     2461 ggcgctcgag ctcggcgcgc aggcgcgcgc gcggcacttc gctcaccgtg agcctgccgc
     2521 tcgcgagcga gatccacgcg attcccgcga cgtcgcccgc ggcgtgcgcc gcgacgagga
     2581 ggctctcgcg cgaggcgggg agcagggccg aatcggtcag cgtccccggc gtcacgacgc
     2641 gcatcacgcg ccgctccacg ggtcccttcg ccgtcgccgg gtcgcccacc tgctcgcaga
     2701 tcgcgaccga ctcgcccgcc ttcacgagct tcgcgaggta gtgctcgacc gagtggtacg
     2761 gcacgcccgc catgcggatc ggctgccccg cggattggcc gcgcgtggtg agcgtgatgt
     2821 cgaggagctt cgccgcgcgc tcggcatcgg catggaacat ctcgtagaaa tcgcccatgc
     2881 ggtagaagag cagcatgtcg gggtactccg ccttgagggc gaggtactgc tgcatcatcg
     2941 gggtgtgggc ggagccgggc gcttcgagct tcatcggcgc ctattgtaag tcgctcccga
     3001 tcccactcgc gctggtgggg tgagtcccgg atgaggcagg catcatcccc agcgggagca
     3061 aacccactaa tccactaatg cagcttcact tttggctcgg tctggcggcg gatccagcgc
     3121 gcgagcgtat cgagcgccat ccgccagaat ccgaagagcg cccagatatg ctgccggtag
     3181 agcgacaggt agaagagctt cgcgagccat ccctcgacgc ggaatttttc gctcgagatg
     3241 aatcccatca gctgcccgat ggtgtcgtag ccgccgagcg agacgaggga cccgaagtcg
     3301 cgatagcggt aggggcgcgg ggccgcgccc gcgaggcgcc gctcgatcgt gcgcacgaga
     3361 tggctcgcct gctggtgcgc ggtctg
//
LOCUS       DASVII010000260         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_13093, whole
            genome shotgun sequence.
ACCESSION   DASVII010000260 DASVII010000000
VERSION     DASVII010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13093"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VF309_03365"
     CDS             complement(<1..247)
                     /locus_tag="VF309_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465655.1"
                     /translation="MPSELAPELQALDRRFVLAKVSVAVTGGDISLETPRSADELINE
                     IDFERDERLPYWADVWPSSTALASAVSALDGKGKRALE"
     gene            complement(237..1499)
                     /gene="glp"
                     /locus_tag="VF309_03370"
     CDS             complement(237..1499)
                     /gene="glp"
                     /locus_tag="VF309_03370"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7465656.1"
                     /translation="MEKPPGERAPLQVAEAVARILAEVRALGPVKRPLLDAYNSVLAA
                     DAVAPVTLPPWDNSAMDGYAVRSADLARLPATLRVIETVAAGQSPTRRVGAGEATRVM
                     TGAPVPEGIDSVVRQEDTDGGTATVEIRDARDTGKNVRPRGEDIVEGHVAIAAGTPIG
                     AAQIGVLASLGFAEVEVVRAPRVAILTSGDELVELDGFAQVRAGKKIVSSNGYTLQTL
                     VRAAGAEPVYLGIAHDTPASLRALLEKARSCDMIITSAGISVGEHDHMRGVLKAMGAE
                     MKFWRVRMRPGAPIGFGLLDGKPWIGLPGNPVSTMVTFELFARPAIHKLRGHRKLFRR
                     PVQVVLDEAITTNADLTHFLRAIVTVRGGIHTARLTGPQSSGILTSMSNANALLVVPP
                     DRRSVAAGETLNAILIGDDAELASDFAL"
BASE COUNT          237 a          531 c          525 g          248 t
ORIGIN      
        1 gttcgagcgc gcgtttgccc tttccgtcga gcgctgagac ggcgctcgcg agcgccgtcg
       61 acgacggcca gacgtcggcc caatacggca ggcgctcgtc gcgctcgaag tcaatctcgt
      121 tgatcagttc atccgcgctg cgcggcgtct cgagggaaat gtcgccgccg gtgaccgcga
      181 cactcacctt cgcgagcacg aagcgccggt cgagcgcctg cagctcgggc gcgagttcag
      241 agggcaaagt cgctcgcgag ttccgcgtcg tcgccaatca gaatggcgtt cagtgtttcg
      301 cccgcggcca cgctccggcg atcgggcgga accacgagca gcgcgttcgc gttcgacatc
      361 gacgtgagaa tccccgagct ctgcgggccg gtgagacgcg ctgtatggat tccgccgcgc
      421 accgtcacga tcgcgcgcag aaaatgcgta aggtcggcgt tcgtcgtgat cgcctcgtcg
      481 agcaccacct gcacagggcg ccggaacagt ttgcggtgtc cgcgcagctt gtggatcgcg
      541 gggcgcgcga acaactcgaa cgtcaccatc gtcgagacgg ggttgcccgg cagcccgatc
      601 catggcttgc cgtcgagcag tccgaatccg atcggcgcgc cgggccgcat gcgcacgcgc
      661 cagaacttca tctcggcgcc catcgccttc agcacgccgc gcatgtggtc atgttcaccg
      721 acggagattc ccgcggaggt gatgatcatg tcgcacgaac gcgccttctc gagcagcgcg
      781 cgcagtgatg caggtgtgtc gtgcgcgatg ccgaggtaca ccggttcggc gccggccgcg
      841 cgcacgagcg tctgcagcgt gtaaccgttg gacgaaacga tcttctttcc cgcgcgaacc
      901 tgagcgaagc cgtcgagttc gacgagctcg tcacccgacg tgagaattgc aacgcgcggc
      961 gcgcgcacga cttcgacttc cgcgaagccg agcgaagcga gcacgccgat ctgcgcggcg
     1021 cctatcggcg ttcccgccgc gatcgccacg tggccttcga cgatatcttc gccgcgcggc
     1081 cgcacattct tgccggtatc gcgcgcgtcg cggatctcca cggtcgcggt gccgccgtcg
     1141 gtgtcttcct gccgcaccac ggaatcgatg ccctcgggaa ccggcgcgcc ggtcatgacg
     1201 cgcgtcgctt cgcccgcgcc gacgcgccgt gtgggcgact gtcccgcggc cacggtctcg
     1261 atcactcgca gggtcgcggg caggcgggcg agatccgccg aacggacggc atatccgtcc
     1321 atcgcggagt tgtcccaggg cggtagcgtc accggcgcga cggcgtcggc agcgaggacc
     1381 gagttgtacg cgtcgaggag cggccgcttg accgggccga gcgcacggac ctcggcgagg
     1441 attcgcgcga cggcctcggc gacctgcagc ggggcacgct cgcccggcgg tttctccaag
     1501 agtcagcgtc tcccggtgtt gctcgacgcg agcagcacga g
//
LOCUS       DASVII010000261         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_264427,
            whole genome shotgun sequence.
ACCESSION   DASVII010000261 DASVII010000000
VERSION     DASVII010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264427"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VF309_03375"
     CDS             <1..721
                     /locus_tag="VF309_03375"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916341.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEX7465657.1"
                     /translation="VGPVAVAKHLEKFLPGHRFLGSGGQSIGAVTAAPFGSASILPIS
                     WMYLEMMGDAGLTAASEVAILNANYIARRLAPHYPVLYSGPRGLVAHECILDLRALKE
                     ASGIQVEDVAKRLIDYGFHAPTMSFPVAGTLMVEPTESESKAEIDRFCDAMIAIREEI
                     RAVEEGRMDREDNPLKTAPHTAEVVSADAWDRKYSRELAAYPLKSLRAEKYWAPVGRA
                     DNVYGDRNLFCSCIPVSDTKE"
     gene            769..1512
                     /gene="surE"
                     /locus_tag="VF309_03380"
     CDS             769..1512
                     /gene="surE"
                     /locus_tag="VF309_03380"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX7465658.1"
                     /translation="MRILLSNDDGYFAPGLAALAEALAPLGQITVVAPERDRSGASNS
                     LTLDRPLMIAKAHNGFHFVNGTPTDCVHMAVTGFLDFEPDVVISGINSGSNMGDDTLY
                     SGTVAAATEGYLLGIPSIAVSLVGKEFAHYATAARVAHDLVKRLHASPFGAPVLLNVN
                     VPDMAFESLAGIEVTRLGRRHKAQPVVAGKNPRGETVYWVGPAGAAREAGPGTDFHAL
                     ERGAVSVTPLQVDLTHARQIPLVSKWIVA"
     gene            1509..>1982
                     /gene="pcm"
                     /locus_tag="VF309_03385"
     CDS             1509..>1982
                     /gene="pcm"
                     /locus_tag="VF309_03385"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028466.1"
                     /note="catalyzes the methyl esterification of
                     L-isoaspartyl residues that are formed in damaged
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX7465659.1"
                     /translation="MSGRDGIGMTSERTRMRMIEALRADKIVNERVLGAMAEIPRHLF
                     VDEGIASRAYEFNTALPIGHGQTISAPIIVALMTQLLLEKSPMNKVLEIGTGCGYQTA
                     VLAKLVKEVYTLERIAPLMDKARRHLRDLRFYNVRYKHADGHDGYPEGAPYDGILM"
BASE COUNT          335 a          683 c          650 g          314 t
ORIGIN      
        1 cgtgggaccg gtggcggtgg cgaagcacct cgagaaattc ctgccgggac atcgcttcct
       61 cggctcgggc gggcaatcca tcggcgcggt gacggcggcg cccttcggca gcgcctcgat
      121 cctcccgatc tcctggatgt acctggagat gatgggcgat gcgggcctca cggcggcgag
      181 cgaggtcgcg atcctcaacg ccaattacat cgccaggcgg cttgcgcccc actatcccgt
      241 gctctactcg gggccgcgag ggctggtcgc ccatgagtgc atcctcgacc tgcgcgcgct
      301 caaggaagcc tcgggaatcc aggtggagga cgtcgccaag cgcctcatcg actatggctt
      361 ccacgcgccc acgatgtcgt tccccgtcgc gggcacgctg atggtcgagc ccaccgagag
      421 cgagtcgaag gccgagatcg accgcttctg cgacgccatg atcgccatcc gcgaggagat
      481 ccgcgcggtc gaggaaggac gcatggaccg cgaggacaac ccgctgaaga ccgcgccgca
      541 caccgcggag gtggtcagcg ccgacgcgtg ggatcgcaag tattcgcgcg agctcgccgc
      601 gtatccgttg aaaagcctgc gcgcggagaa gtattgggct cccgtcggcc gcgcggacaa
      661 cgtctacggc gaccgcaacc tcttctgctc ctgcattccg gtcagcgaca ccaaggagtg
      721 accgcaaagg ggcgctggca cctcgctcaa gctataattt tgcctcgcat gcgcatcctc
      781 ctctcgaacg acgacggcta tttcgcccca ggactcgccg ccctcgccga ggccctcgcg
      841 ccgcttgggc agatcaccgt ggtcgcgccc gagcgggatc gcagcggcgc gtcgaactcc
      901 ctcacgctcg atcgtccgct gatgatcgcc aaggcccaca acggcttcca tttcgtgaac
      961 ggcacgccga ccgactgcgt ccacatggcg gtcacgggat ttctcgattt cgagccggac
     1021 gtagtgatct cggggatcaa cagcggctcc aacatgggcg atgacacgct ctactcgggg
     1081 accgtggccg cggcgaccga gggctatctt ctcggcatcc cgtcgatcgc ggtgtcgctc
     1141 gtcggcaagg agttcgcgca ctacgcgacc gcggcacgcg tcgcccacga cctggtgaag
     1201 cggctgcacg cctcgccctt cggcgctccc gtgctcctca acgtgaacgt tcccgacatg
     1261 gccttcgagt cgctcgcggg catcgaggtc acgcgcctgg gccgcaggca caaggcgcag
     1321 cccgtcgtgg cgggcaagaa tccgcgcggc gagaccgtgt actgggttgg tcccgcgggg
     1381 gcggcgcgcg aagcgggacc cggcaccgat ttccacgcgc tcgagcgcgg cgccgtgtcg
     1441 gtgacgccgt tgcaggtgga cctcacccac gcccggcaga ttcccctggt atcgaaatgg
     1501 atcgtggcgt gagcgggcgc gacggcatcg gcatgacctc cgagcgcacg cgcatgcgca
     1561 tgatcgaggc gctgcgcgcc gacaagatcg tgaatgagcg cgtgctgggc gcgatggcgg
     1621 aaatcccgcg ccacctgttc gtcgacgagg gcatcgcctc gcgcgcctac gagttcaaca
     1681 ccgcgcttcc catcggccac ggacagacga tctctgcgcc catcatcgtc gccctcatga
     1741 cgcagctgct gctggagaaa tcgccgatga acaaggtgct cgagatcggc acgggctgcg
     1801 gctaccagac ggcggtgctg gcgaagctcg tgaaggaggt ctacaccctc gagcggatcg
     1861 cgccgctcat ggacaaggcg cggcgccacc ttcgagacct gcgtttctac aacgtgcgct
     1921 acaagcacgc cgacgggcac gacggctatc ccgagggcgc gccgtacgac ggtatcctga
     1981 tg
//
LOCUS       DASVII010000262         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_588104,
            whole genome shotgun sequence.
ACCESSION   DASVII010000262 DASVII010000000
VERSION     DASVII010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588104"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF309_03390"
     CDS             complement(<1..190)
                     /locus_tag="VF309_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465660.1"
                     /translation="MWRKVETVGEGKGAYFIRSGNPEEVKFHTRAMMRCAIDTKVNQQ
                     ESSLAVTPAARRLDHEDVA"
     gene            233..1249
                     /gene="dusB"
                     /locus_tag="VF309_03395"
     CDS             233..1249
                     /gene="dusB"
                     /locus_tag="VF309_03395"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX7465661.1"
                     /translation="MKIGSIQLKNNLIVAPMAGVTDRPFRSLCKKLGAGMAVSEMVAS
                     NSLLWGSEKTIRRGNHEGEVEPKVIQIAGSDPAMMAEAAKYNVDSGAHIIDINMGCPA
                     KKICNVFAGSALLQDEPLVARIVDAVVRAVNVPVTLKIRTGWNKDNRNALRIAKIAED
                     HGIQSLAMHGRTRACMYTGDAEYDTIRAVKQSVKIPVVANGDVRTPEKAKFVLEYTGA
                     DAVMIGRAAQGRPWIFREIQYFLETGAHLPPPTVAEIREVLVGHLHDLYSFYGYERGT
                     RVARKHISWYTKGLKNSAVFRARMNTLESSELQLAAVASFFGELTAHSARLEYVEDEV
                     ALAA"
     gene            1317..1511
                     /locus_tag="VF309_03400"
     CDS             1317..1511
                     /locus_tag="VF309_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7465662.1"
                     /translation="MRQYFKDLDGEKASGIYDMVVLAVEKPMLEVVMFQAHGNQTRAA
                     ELLGMNRNTLRKKLQQHGLD"
     gene            1517..>2933
                     /gene="purH"
                     /locus_tag="VF309_03405"
     CDS             1517..>2933
                     /gene="purH"
                     /locus_tag="VF309_03405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7465663.1"
                     /translation="MKIQRALISVSDKTGVVELARALLTHGVQILSTGGTAQLLRLNG
                     VVVTEVAEHTGFPEMMEGRVKTLHPKVHGGILARRDSPSHLQSMREHDIAPIDMVVVN
                     LYPFAQTVAKPGCTLEDAIENIDIGGPAMVRAAAKNHEFVAVVTDPADYPSIIAKLAS
                     GRGKLDFAERFRLAAKAFSHTAEYDGMISNWLTARSPQGVAREFPDRLNLQMQLSQAL
                     RYGENPHQSAAFYVERDPAPGTLARFKQLQGKELSYNNIADSDAAWECCRTFEVPACV
                     IVKHANPCGVAVADSPLAAYRNAFLTDPTSAFGGIIAFNRAVDGATAEAVARQFVEVV
                     IAPSYTPEALAAFGAKKNVRVLSIGIARDLNRLDMKRVGGGLLVQSSDAFDVAELRIV
                     TKRDPTKDEMNDLVFAFRVAKFVKSNAIVFARHGMTLGVGAGQMSRVDSTRIAAIKAK
                     NAGLSLRGSVAASDAFFPFRDG"
BASE COUNT          540 a          982 c          941 g          470 t
ORIGIN      
        1 cggcgacgtc ttcgtgatcg agacgccggg cggcgggggt tacggcgagg cttgattctt
       61 gctggttgac ttttgtgtca atcgcgcaac gcatcatcgc gcgtgtgtga aatttcactt
      121 cctcgggatt tccgctgcgt ataaaatagg cgcctttccc ttcgccgaca gtctcgactt
      181 ttcgccacac ggtctcgacg agacgccaca ccaaaagagc ggcgcagcca acttgaaaat
      241 cggttccatc cagctcaaga acaacctgat cgtcgcgccc atggcgggcg tgaccgatcg
      301 acctttccgc agcctctgca agaagctggg cgcgggaatg gccgtgtccg agatggtcgc
      361 gtcgaattcg ctgctgtggg gctcggagaa gaccatccgc cgcggcaacc acgagggcga
      421 agtcgagccc aaggtgatcc agatcgccgg atcggacccc gcgatgatgg ccgaggccgc
      481 gaaatacaac gtcgacagcg gcgcccacat catcgacatc aacatgggct gccccgccaa
      541 gaagatctgc aacgtgttcg cgggctcggc gctattgcag gatgagccgc tcgtggcacg
      601 catcgtggac gcggtggtgc gcgcggtgaa cgtgcccgtc accctcaaga tccgcaccgg
      661 ctggaacaag gacaaccgca acgcgctgcg catcgcgaag atcgccgagg accacggcat
      721 ccaatcgctc gccatgcacg ggcgcacgcg cgcctgcatg tacacgggcg atgcggaata
      781 cgacaccatc cgcgcggtga agcagtcggt gaagatcccg gtcgtcgcca acggcgacgt
      841 gaggactccc gagaaggcga agttcgtcct cgagtacacc ggcgccgacg cggtgatgat
      901 cggccgcgcc gcccagggcc gcccctggat cttccgcgag atccagtatt tcctcgagac
      961 cggcgcgcac ctcccgccgc cgacggtcgc ggaaatccgc gaggtgctgg tgggccacct
     1021 gcacgacctg tattccttct acggctacga gcgcggcacg cgggtggcgc gcaagcacat
     1081 cagctggtac acgaaaggac tcaagaactc cgccgtgttc cgtgcccgca tgaacaccct
     1141 cgagagctcc gaattgcagc tcgccgccgt ggcatcgttc ttcggcgagc tcaccgccca
     1201 ttcggcgcgc ctcgaatatg tcgaagacga ggtggctctt gcagcctaga aacagatccg
     1261 acgcgagagc ggacgccgtt cgcgagaacg agctttcctc cacggtgcgc aaggtgatgc
     1321 gccagtactt caaggacctc gacggggaga aggcctcggg catctacgac atggtggtcc
     1381 tggccgtcga gaagccgatg ctcgaagtcg tgatgttcca ggcccacggc aaccagacgc
     1441 gcgccgcgga gctgctgggg atgaaccgca acaccctgcg caagaagctg caacagcacg
     1501 ggctggacta gtcacgataa agatccagcg cgccctcatc agcgtctccg acaagaccgg
     1561 cgtggtcgag ctggcgcggg cgctgctcac gcacggcgtg cagatcctct ccacgggcgg
     1621 caccgcgcag ctcctcaggc tgaacggcgt cgtcgtcacc gaagtcgcgg agcacacggg
     1681 ctttcccgag atgatggaag gccgcgtgaa gacgctgcac ccgaaggtgc acggcggcat
     1741 cctcgcgagg cgcgacagcc cgagccacct gcagtcgatg cgcgagcacg acattgcgcc
     1801 gatcgacatg gtggtcgtga acctctatcc gttcgcgcag accgtggcca agcccggctg
     1861 cacccttgaa gacgcgatcg agaacatcga catcggcggc cccgcgatgg tgcgcgcggc
     1921 ggcgaagaac cacgagttcg tggcggtggt gaccgatccg gcggactatc cgtcgatcat
     1981 cgcgaagctc gcgtcgggca ggggcaagct cgatttcgcc gagcgcttcc gcctcgccgc
     2041 gaaggccttc agccacacgg ccgagtacga cggcatgatc tccaactggc tcacggcgcg
     2101 cagcccccag ggcgtggcgc gggaattccc cgaccggctc aacctgcaga tgcagctttc
     2161 ccaggcgctt cgttacggag agaatccgca ccagtcggcg gcgttctacg tcgagcgaga
     2221 tcccgcgccc ggcacgctcg cccgcttcaa gcagctgcaa ggcaaggagc tcagctacaa
     2281 caacatcgcc gactccgacg cggcgtggga gtgctgccgc acgttcgagg tgccggcgtg
     2341 cgtcatcgtg aagcacgcca atccgtgcgg cgtcgcggtg gcggattcgc cgctcgcggc
     2401 ctatcgcaac gcgttcctca ccgatccgac ttccgcgttc ggcggcatca tcgcgttcaa
     2461 ccgcgcggtg gacggcgcga ccgccgaggc ggtcgccagg cagttcgtcg aggtggtgat
     2521 cgccccttcg tacacgcccg aggccctcgc ggccttcggc gcgaagaaga acgtgcgcgt
     2581 gctctccatc gggatcgcgc gcgacctcaa tcggctcgac atgaagcggg tcggcggcgg
     2641 cctcctggtg cagtcttccg acgccttcga cgtggccgag cttcgcatcg tcacgaagcg
     2701 cgatccgacg aaggacgaga tgaacgacct cgtcttcgcg ttccgcgtgg cgaagttcgt
     2761 gaagtccaac gcgatcgtct tcgcgcgcca cggcatgacg ctcggcgtgg gcgcgggcca
     2821 gatgagccgc gtcgattcca cgcggatcgc ggccatcaag gcgaagaatg ccggcctttc
     2881 tctgcgcggc tccgtggccg catccgacgc gttctttccg ttccgcgacg gcc
//
LOCUS       DASVII010000263         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_444224,
            whole genome shotgun sequence.
ACCESSION   DASVII010000263 DASVII010000000
VERSION     DASVII010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444224"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VF309_03410"
     CDS             complement(<1..920)
                     /locus_tag="VF309_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX7465664.1"
                     /translation="MSQADGLAHLVEEGIIDAVHARLMSGKEATVYVVEREGHLGAAK
                     VYKTRDERTFKKTASYVEGRNQTRNTRDRRAMQNKSAYGRGLIEDNWQDMEFHALRGA
                     FDAGVRVPEPYFLYENVLFMELIVDAAGAPASRLADFTFTSEEATRLHQEVFLQVKTL
                     LGTGRIHGDLSAYNVLMAAKGPTLIDMPQIVEVSGNNNARQILRRDLRNVTEHLARFD
                     ARLLRFADCGDGLFHHMMRGTLDMVAGPEVGAPQARGGGGGGGRRGRDAERGRAGRDK
                     GQRPGLNAGANPARGGGAAMARPGGGGSPA"
     gene            complement(951..1157)
                     /locus_tag="VF309_03415"
     CDS             complement(951..1157)
                     /locus_tag="VF309_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465665.1"
                     /translation="MKDNLFTEVYLSTSVSTPIQLKGWVNQANQFERHGQRWHLYLSR
                     VEILRRLATRNRKNLASSVESRPA"
     gene            complement(1282..>1881)
                     /locus_tag="VF309_03420"
     CDS             complement(1282..>1881)
                     /locus_tag="VF309_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7465666.1"
                     /translation="LYELFMGEFELPKPWDPRAIEGVNRFLKRAWRLCEEWDAARAPA
                     GDPHLRLRHKTIRRVTADLERMQFNTAISALMEYVNEMVSGGATREDLLTLIKLVGPY
                     APHLGDEAWERLGERGFLLDAAWPTFDQALTIDAQVTVGVQVDGKLRGSVELPRDASE
                     DDARAAALAIPNVAKHLEGRVMKKFIYKPGRIIGIVTGP"
BASE COUNT          265 a          639 c          640 g          337 t
ORIGIN      
        1 ctcgccggcg acccgccgcc gcccggcctc gccatcgccg caccgccgcc cctcgccgga
       61 ttcgcgccag cgttcaagcc cggccgttgc cccttgtcgc gacccgcgcg tccccgctcc
      121 gcgtcgcgtc cccgccgccc accgccaccg ccaccgcccc gagcctgcgg cgcgccgact
      181 tcggggcccg cgaccatgtc cagcgttccg cgcatcatgt ggtggaacaa tccgtcgccg
      241 cagtcggcga agcggagcag ccgcgcgtcg aatcgcgcca ggtgctcggt cacgttgcgc
      301 agatcgcggc gcaggatctg gcgggcgttg ttgttgcccg acacctcgac gatctggggc
      361 atgtcgatga gggtcggccc cttcgccgcc atgaggacgt tgtaggcgga cagatcgccg
      421 tggatgcgcc cggtgccgag cagcgtcttg acctgcagga acacctcctg atgcagccgc
      481 gtcgcctcct cggaggtgaa ggtgaaatcg gccagccgcg aggcgggcgc gcccgccgcg
      541 tcgacgatca gctccatgaa gaggacgttc tcgtagagga agtacggctc cgggacgcgc
      601 acgccagcgt cgaacgcgcc gcgcagcgcg tggaactcca tgtcctgcca gttgtcctcg
      661 atgaggccgc ggccgtaggc gctcttgttc tgcatcgcgc ggcggtcgcg ggtgttgcgg
      721 gtctggttcc gcccctccac gtagctggcg gtcttcttga acgtgcgctc gtcgcgcgtc
      781 ttgtagacct tggccgcgcc gaggtgccct tcgcgctcca cgacgtagac cgtcgcttcc
      841 ttcccgctca tcagacgcgc gtggacggcg tcgatgatcc cttcttcgac gagatgggcg
      901 aggccgtcag cttgggacat tcgtcgccgg tgaaggcctc gggtcgttcc tcaggcggga
      961 cgactctcga cgctcgacgc gaggttcttc cggttgcgcg tggccaaccg tcgcaagatc
     1021 tcgacgcgcg acaggtaaag gtgccagcgc tggccgtgcc gctcgaactg gttggcctgg
     1081 ttcacccagc ctttgagctg gatcggcgtg ctcaccgacg tcgagaggta gacctccgtg
     1141 aagaggttgt ctttcatggc gcgcttcgcc tcggcgtaac tctcgaaaaa attgtcgggc
     1201 atggcgaggc ttctaccaga atttcgcacg ccgatgtagg tttatgcata caggcataga
     1261 tttacgtacg cgcgcgtacg tttacgggcc ggtgacgatt ccgatgatcc gccccggctt
     1321 gtagatgaat ttcttcatca cgcgcccctc gaggtgcttg gcgacgttcg ggatcgcgag
     1381 cgccgctgcc cgcgcgtcgt cctcggaggc gtcgcgcggg agctcgacgc tcccgcgcag
     1441 cttgccgtcg acctggacgc cgacggtgac ctgcgcgtcg atggtcagcg cttggtcgaa
     1501 ggtgggccag gccgcgtcga gcaagaagcc gcgctcgccg aggcgctccc aggcctcgtc
     1561 gcccaggtgg ggcgcgtagg gaccgaccag cttgatcagc gtcaggagat cctcgcgcgt
     1621 ggcgccgccc gagaccatct cgttgacgta ttccatgagg gcggagatgg cggtgttgaa
     1681 ctgcatccgc tccagatccg cggtcacgcg gcggatcgtc ttgtggcgta ggcgcaggtg
     1741 cggatcgcca gcgggcgcgc gggcggcgtc ccactcctcg cagaggcgcc aggcgcgctt
     1801 gaggaagcgg ttgacccctt cgatggcgcg cgggtcccag ggcttgggga gctcgaactc
     1861 gcccatgaag agctcgtaga g
//
LOCUS       DASVII010000264         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_516216,
            whole genome shotgun sequence.
ACCESSION   DASVII010000264 DASVII010000000
VERSION     DASVII010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516216"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VF309_03425"
     CDS             complement(<1..1036)
                     /locus_tag="VF309_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7465667.1"
                     /translation="MANPLLSRWSGPHGGVPPFDKVTVEHFRPALEAAMEECLAEIDR
                     IAGDPAPPDFENTIAAFERAGHTLDRVRRVYSVFSSTLRSPAFQQVEREMEPKMAAFR
                     DRILQNAKLFERISAVYATRASSQLTAEQQRLAWLKYTDFARGGARLEPGAKQRLSEI
                     NQRLASLYTQFSQNVLADEGTALILDSAADLAGLPESVRSAAAEAAGERGQPGKWAIL
                     NTRSSMEPFLAYSGRRDLREKVWRAYYSRGDNGDARDNKNIIAEIVRLRGERATLLGY
                     QSHAHWRLEVSMAKTPERATQLMEAVWRPAVKRVAEEVADMQAEADREGAGLPGGLST
                     RITIEPWDYRY"
     gene            complement(1046..1972)
                     /locus_tag="VF309_03430"
     CDS             complement(1046..1972)
                     /locus_tag="VF309_03430"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7465668.1"
                     /translation="MRLESSAKGVYVIAPTPFHPDGRIDEGSIDRMTDFFVGAGVNGM
                     TVLGQLGEAPKMEHGESVGIVKRVIGRTTVPVIVGVSAPGFAAMRALTQDVMAAGAAG
                     VMIAPPNTLRTDDSIVQYYRQASEAIGADVPFVIQDYPLTFSVQMAPGVIRRIVMENP
                     ACKVLKHEDWPGLEKISQLRAWEKDGSMRRISILTGNNALFLDFEMERGADGANTGYC
                     FPDMLVDVVRMSAAGERDAAHDLFDAHLPLIRYEQQPGPGLAVRKYVMMRRGIIASDT
                     LRKPGAALTPAARAEVDYLLSRLAQKDRRAKV"
BASE COUNT          340 a          729 c          736 g          379 t
ORIGIN      
        1 agtagcggta gtcccacggc tctattgtga tgcgggtcga aaggcccccc gggagaccgg
       61 cgccttcgcg gtcggcctcg gcctgcatgt cggcgacttc ctccgcgacg cgcttcaccg
      121 cgggacgcca caccgcctcc atgagttgcg tggcgcgctc cggcgtcttc gccatgctca
      181 cttccaggcg ccagtgggcg tggctctggt agccgagaag cgtcgcgcgc tcgcctcgca
      241 ggcgcacgat ctcggcgatg atgttcttgt tgtcgcgcgc gtcgccgttg tcgccccggc
      301 tgtagtacgc gcgccagacc ttctcgcgca ggtcgcggcg tcccgaatag gcgaggaagg
      361 gctccatgct cgagcgcgtg ttgaggatcg cccattttcc cggctgcccg cgctccccgg
      421 cggcctcggc cgcggcggag cgcaccgact cgggcaagcc cgccaggtcc gccgccgaat
      481 cgaggatcag cgccgtcccc tcgtcggcga gcacgttctg gctgaactgc gtatagagcg
      541 acgcgagccg ctggttgatc tccgaaaggc gctgcttcgc gccgggctcg aggcgcgcgc
      601 cgccgcgggc gaagtccgtg tacttgagcc aggcgaggcg ctgctgctcc gccgtgagct
      661 gcgagctcgc acgcgtcgcg tagaccgccg agatgcgctc gaaaagcttc gcgttctgca
      721 ggatgcgatc gcggaacgcg gccatcttcg gctccatctc gcgctcgacc tgctggaacg
      781 cgggcgagcg cagggtcgag ctgaagacgc tgtagacgcg cctgacgcga tcgagcgtgt
      841 ggcccgcgcg ctcgaaggcg gcgatggtgt tttcgaaatc gggcggcgcg ggatcgccgg
      901 cgatgcggtc gatctccgcg aggcactcct ccatcgccgc ctcgagcgcc ggcctgaagt
      961 gctcgaccgt caccttgtcg aacgggggga ctccgccgtg gggaccgctc cagcgggaaa
     1021 gaaggggatt cgccatcgct cgcctctaga ctttcgcgcg ccgatccttt tgggccaggc
     1081 gcgataaaag atagtcgacc tcggcgcgcg ccgccggggt gagggccgcg ccgggctttc
     1141 ggagtgtatc ggatgcgatg atgccacggc gcatcatcac gtacttgcgc accgcgaggc
     1201 cggggccggg ctgctgctcg tagcggatga gcggcaggtg cgcgtcgaaa aggtcgtggg
     1261 cggcgtcgcg ctcgcccgcg gccgacatcc gcacgacgtc caccagcatg tcggggaagc
     1321 aatagccggt gttggccccg tcggcgccgc gctccatctc gaaatcgagg aagagggcgt
     1381 tgttgcccgt gaggatcgag atgcggcgca tcgagccgtc cttctcccag gcgcgcagct
     1441 gcgagatctt ctcgaggccg ggccagtcct cgtgcttgag caccttgcac gcggggttct
     1501 ccatgacgat gcgccggatc acgccgggcg ccatctgcac cgagaacgtg agcggatagt
     1561 cctggatcac gaaaggcacg tccgcgccga tcgcttccga cgcctggcgg tagtactgca
     1621 cgatcgagtc gtcggtgcgc agcgtattcg gcggcgcgat catcacgccc gcggcgcccg
     1681 cggccatcac gtcctgcgtg agcgcgcgca tggccgcgaa gcccggggcg gagaccccca
     1741 cgatgaccgg cacggtcgtg cgcccgatga cgcgcttcac gatgccgacg gactcgccgt
     1801 gctccatctt cggcgcctcg ccgagctggc ccagcacggt catgccgttc acgccggccc
     1861 cgacgaagaa gtccgtcatc cggtcgatcg agccttcgtc gatgcgcccg tcgggatgga
     1921 acggcgtcgg cgcgatcacg tacacgccct tcgcgctgct ctccagtctc attccatttc
     1981 tcttaaagga ctcaagatct atccgcagat aaacgcagat gaacgcggta agagcaaagg
     2041 catatcggaa tttcgtgcga cgggcccagt atctcgaaat ttcaatcggt tttggggttg
     2101 ccgcgttcat ctgcgtctat ctgcggatag gccttttagc catttgaacc aaatgcaatg
     2161 aaactcgaga gcaacgcgaa gggc
//
LOCUS       DASVII010000265         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_480295,
            whole genome shotgun sequence.
ACCESSION   DASVII010000265 DASVII010000000
VERSION     DASVII010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480295"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VF309_03435"
     CDS             complement(<1..1276)
                     /locus_tag="VF309_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX7465669.1"
                     /translation="MEAFLEQTFGTIPELVRMHAARAPRHPALIQDERSIDFGALDAL
                     MDRIAAAFQRDGLEPGRAVAICAANSIEYAAVFLGALRAGLAVAPLAPSSTPDAIAAM
                     AADAGASHLFLDEAAAKAMAPVLAQMTARAIALGGLSQWIAGESTPRAIEAAPGDPFN
                     IIYSSGTTGIPKGIVQPNAMRWAHVVRSAVYGYGPEAITLVSTPLYSNTTLVSFFPTV
                     ALGGTAVLMAKFEAAAYLALAERHRVTHTMLVPVQYQRIMALADFARFDLSSFRVKFS
                     TSAPFAAALKADIVARWPGALYEYYGMTEGGGTCVLFAHEFPKKLHTVGRPAPGVDLR
                     LIDEEGREVAPGEVGEVVGHSVAMMTGYHRRPEMTSSAEWFDSEGKRFIRTGDIGRFD
                     DDGFLVLLDRKKDLVISGGFNVYPSDLEAVLAR"
     gene            complement(1280..2059)
                     /locus_tag="VF309_03440"
     CDS             complement(1280..2059)
                     /locus_tag="VF309_03440"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7465670.1"
                     /translation="MSLKALFDLSGKSALVTGGSRGLGLQMAEALGELGAKVALTARK
                     RDELDEAVAHLASMGVKAIPIVCDMSQPNAIAPAVEQAIAALGPIDILVNNAGTTWGA
                     ATIDHPLDAWQKVINLNLTAMFVVSQEVGKRCMVPRRSGKVINIASILGLVGGGDPGR
                     PGTIAYNTSKGGVVNFTRSLAAEWAQYNINVNAIAPGFFPTKMTKGLMEIMGDEVANK
                     APLKRVGGPEDLKGIAALFASDACAFITGQIVAVDGGVTAI"
     gene            complement(2076..>2325)
                     /locus_tag="VF309_03445"
     CDS             complement(2076..>2325)
                     /locus_tag="VF309_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7465671.1"
                     /translation="GGPPIEGSTWVLFLAPAGTPREIVNRISSETAKIVATPEMRARF
                     EQLGIEPVGSTPAEAGRFLDDEIAKWAKVINIAGVKPE"
BASE COUNT          355 a          780 c          816 g          374 t
ORIGIN      
        1 ggcgggcgag caccgcttcg agatcgctcg ggtacacgtt gaagccgccg gagatgacga
       61 ggtccttctt gcggtcgagg aggacgagga agccgtcgtc gtcgaagcgc ccgatgtcgc
      121 ccgtgcggat gaagcgcttg ccctcgctat cgaaccattc ggcgctcgac gtcatctcgg
      181 gccgccggtg gtagccggtc atcatcgcca ccgagtggcc gacgacttcg cccacttctc
      241 ccggcgccac ctcgcggccc tcctcgtcga tgaggcgaag gtccacgccc ggcgcggggc
      301 gtcccaccgt gtgcagcttc ttcggaaact cgtgggcgaa taggacgcag gtgccgccgc
      361 cctcggtcat cccgtagtac tcgtagaggg ccccgggcca gcgcgcgacg atgtcggcct
      421 tgagcgccgc ggcgaaggga gcgctggtgg agaacttgac gcggaaggag gagaggtcga
      481 agcgcgcgaa atcggcgagc gccatgatgc gctggtactg caccgggacc agcatggtgt
      541 gcgtgacgcg atggcgctcg gcgagcgcga gatacgccgc ggcttcgaac ttcgccatca
      601 gcacggcggt cccgcccagg gccacggtcg gaaagaacga gacgagcgtc gtgttggagt
      661 agagcggcgt cgagacgagc gtgatcgcct cgggaccgta gccgtacacg gcgctcctca
      721 ccacgtgcgc ccagcgcatc gcgttgggct gcacgattcc cttcgggatc ccggtggttc
      781 ccgacgaata gatgatgttg aagggatcgc cgggcgcggc ctcgatcgcg cgcggcgtgc
      841 tctcgcccgc gatccattgc gacaggcccc cgagcgcgat cgctcgcgcc gtcatttgcg
      901 cgagcacggg ggccatcgcc ttcgccgcgg cctcgtcgag aaagaggtgc gaggccccgg
      961 cgtcggcggc catcgcggcg atcgcatccg gggtcgagga cggcgcgagc ggcgccaccg
     1021 cgaggcccgc gcgcagcgcg ccgaggaaga ccgcggcgta ctcgatcgag ttcgccgcgc
     1081 agatcgcgac ggcgcgcccg ggctcgagcc cgtcgcgctg gaacgcggcg gcgatgcgat
     1141 ccatcagcgc gtcgagcgcc ccgaagtcga tcgagcgctc gtcctggatc aacgccggat
     1201 ggcggggcgc gcgcgcggcg tgcatgcgca ccagctcggg aatcgtcccg aaggtctgct
     1261 cgagaaacgc ctccaccgac tagatcgcgg tcacgccgcc gtcgaccgcg acgatctggc
     1321 cggtgatgaa cgcgcacgcg tccgaggcga agagcgcggc gattcccttg aggtcctcgg
     1381 gcccaccgac gcgcttgagg ggagccttgt tcgcgacctc gtcgcccatg atctccatga
     1441 ggcccttggt catcttcgtg ggaaagaagc ccggggcgat cgcattcacg ttgatgttgt
     1501 actgcgccca ttccgcggcg agcgagcgcg tgaagttcac gacgccaccc ttgctcgtgt
     1561 tgtacgcgat ggtgccgggg cgcccgggat cgccgccgcc gacgaggccg aggatcgagg
     1621 cgatgttgat caccttgccg ctgcgccgcg gcaccatgca gcgctttccc acctcctggc
     1681 tcaccacgaa catcgcggtg agattgaggt tgatcacctt ctgccacgcg tcgagcgggt
     1741 ggtcgatggt ggccgcgccc cacgtggtcc cggcgttgtt cacgaggatg tcgatggggc
     1801 cgagcgccgc gatcgcctgc tcgacggcgg gcgcgatcgc gttcggctgc gacatgtcgc
     1861 acacgatggg aatcgccttc acgcccatgg acgccaggtg cgccacggct tcgtcgagct
     1921 cgtcgcgctt gcgcgcggtg agcgccacct tcgcccccaa ttcgcccagc gcctccgcca
     1981 tctgcaggcc caggccacgg gatccgccgg tgacgagggc ggattttccg gaaagatcga
     2041 acagtgcttt caagctcatt gaaggattcc agttttcatt cgggtttcac gcccgcgatg
     2101 ttgatgacct tcgcccactt ggcgatctcg tcgtcgagga agcgccccgc ctcggcgggg
     2161 gtgctgccga cgggctcgat gccgagctgc tcgaagcggg cgcgcatctc gggagtggcg
     2221 acgatcttcg cggtctcgct ggagatgcgg ttcacgatct cgcgaggcgt tccggcgggc
     2281 gcgagaaaga gcacccacgt cgatccctcg atcggcgggc cgccg
//
LOCUS       DASVII010000266         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_49034, whole
            genome shotgun sequence.
ACCESSION   DASVII010000266 DASVII010000000
VERSION     DASVII010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49034"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="argC"
                     /locus_tag="VF309_03450"
     CDS             complement(<1..811)
                     /gene="argC"
                     /locus_tag="VF309_03450"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7465672.1"
                     /translation="MIKVGVVGGTGYTGVELLRLLAGHGDVEVVAIPSRAEAGVKVAD
                     HFPSLRGHYDLAFTDPATAPLKGCDCVFFATPNGVAMSQARTLLDAGSRVVDFSADFR
                     LKDRATWEKWYKVSHASPELFSEAVYGLPEINRERIREARLVANPGCYATAVQLALLP
                     LVETNHVDHAHLVADGKSGVSGAGRKAEVDLLFSEASDNFKPYAVKGHRHHPEIIESL
                     TSLEGASGRKLGLTFVPHLVPMVRGILATIYARITSEADFQAIFEKRYAKEP"
     gene            complement(928..1320)
                     /gene="rpsI"
                     /locus_tag="VF309_03455"
     CDS             complement(928..1320)
                     /gene="rpsI"
                     /locus_tag="VF309_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7465673.1"
                     /translation="MIGKYNYGTGRRKNAVARVFMKPGKGDIVVNDKPVDVFFSRETG
                     RMIVRQPLELTNHVQTFDIKVNVFGGGESGQAGAVRHGITRALIEYDPTLKPALSKAG
                     LVTRDAREVERKKVGLHKARRRKQFSKR"
     gene            complement(1332..>1520)
                     /locus_tag="VF309_03460"
     CDS             complement(1332..>1520)
                     /locus_tag="VF309_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HEX7465674.1"
                     /translation="PGGIYETNFTKLQARHPDRVLKLAVKGMLPKGRLGYAMIKKLKI
                     YAGPAHPHTAQQPKNLEI"
BASE COUNT          258 a          489 c          486 g          287 t
ORIGIN      
        1 agggctcctt cgcgtagcgc ttctcgaaaa tcgcctggaa atcggcctcg gacgtgatcc
       61 tcgcgtagat cgtggcgagg atgccacgca ccatcgggac caggtgcggc acgaaggtga
      121 gccccagttt gcgtcccgaa gcgccctcga ggctcgtgag gctctcgatg atttcaggat
      181 ggtggcgatg gcccttcacg gcatatggct tgaagttgtc ggacgcctcg gaaaagagga
      241 ggtcgacctc ggccttgcgt cctgcgccgc tcacgcccga cttcccgtcg gcgaccaaat
      301 gcgcgtggtc gacatggttg gtctcgacca gcggcagtaa cgcgagctgc accgcggtcg
      361 catagcatcc ggggttcgcg acgagccgcg cctcgcggat gcgctcgcgg ttgatctccg
      421 gcaatccgta gacggcctcg gagaagagct cgggagacgc gtgggacacc ttgtaccact
      481 tctcccacgt ggcgcgatcc ttcaagcgaa aatccgcgga gaagtcgacg acgcgcgaac
      541 ccgcgtccag cagcgtgcgg gcctggctca tggcgacgcc gttgggcgtg gcgaagaaca
      601 cgcaatcgca tcccttgagg ggcgcggttg cgggatccgt gaacgcaagg tcgtagtgtc
      661 cgcgcagcga cggaaaatga tccgcgacct tcaccccagc ctcggcgcgc gaggggatcg
      721 ccacgacttc gacgtcgcca tggccggcga gcagccgcag cagctcgacg cccgtgtaac
      781 cggtaccgcc tacgactccc accttgatca ttcgatcatt ccttccaggc ccatatgcca
      841 ggcgcaagac ggccattccc agaacgacaa agccgctgcg aggcggcttt gttcgtcaca
      901 gctcttgcaa acgagcgagc accagcctca acgcttcgag aactgcttgc ggcgtcgcgc
      961 cttgtgaagg ccgaccttct tgcgctcgac ttcgcgggcg tcgcgcgtga cgagcccagc
     1021 cttggaaagc gcgggcttca aggtcggatc gtattcgatg agcgcccgcg tgatgccgtg
     1081 gcgcaccgcg cccgcttgcc ccgattcgcc gccgccgaac acgttcacct tgatgtcgaa
     1141 ggtctgcacg tggttggtga gctcgagggg ctggcgcacg atcatgcgtc ccgtctcgcg
     1201 ggagaagaac acgtcgacgg gcttgtcgtt caccacgatg tcgcccttgc cgggcttcat
     1261 gaagacgcgc gccacggcgt tcttgcggcg gcccgtcccg taattgtatt tgccgatcat
     1321 tgtgtggctc cttagatctc gaggttcttg ggctgctgcg ccgtatgcgg atgcgcggga
     1381 cccgcgtaga tcttcagctt cttgatcatc gcgtagccga ggcgtccctt gggcaacatg
     1441 cccttcaccg cgagcttcag cacgcgatca ggatggcgcg cctggagctt ggtgaagttc
     1501 gtctcgtaga ttccgcccgg
//
LOCUS       DASVII010000267         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_480347,
            whole genome shotgun sequence.
ACCESSION   DASVII010000267 DASVII010000000
VERSION     DASVII010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480347"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF309_03465"
     CDS             complement(<1..677)
                     /locus_tag="VF309_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX7465675.1"
                     /translation="MTTDAKPAPRILVKQDTGTHYFFAPCPRGLSGALAEELGELGAG
                     AIEPAEAGVAFSGAFDLVYIANLHSRIASRILWRVAQFPYKSEDDVYNGAAGIRWNSH
                     FNADRTFKIDTNAHRSPVKSLDFITLRVKDAVADQFRAAAGRRPSVESRDPDVRIHVF
                     LDAKMCTLYVDTSGDSLFKRGKRDHVGEAPLKKNLAAGILRLAKWRPGVPLLDPMCGA
                     GTFLSEAA"
     gene            complement(679..1092)
                     /gene="hemJ"
                     /locus_tag="VF309_03470"
     CDS             complement(679..1092)
                     /gene="hemJ"
                     /locus_tag="VF309_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEX7465676.1"
                     /translation="MLWLKALHIIFVVAWFAGLFYLPRLFVYHALSSDAASNERFKVM
                     ERKLYRGIMTPCMALTLVLGTWLWLGYGFTGGWLRAKLVLVVLLVGYHLWLGRLAMDF
                     AADANRRSHVFYRWINELPLVLLAAIVILVVVKPF"
     gene            1112..>1580
                     /locus_tag="VF309_03475"
     CDS             1112..>1580
                     /locus_tag="VF309_03475"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX7465677.1"
                     /translation="MFTLSPVLAAAFAAIALLGSPASRAATLVKTEHVEADLVADKAS
                     AQPGKPLRVGLRLRMEPQWHTYWRNPGDSGLPTKIQWILPEGWKAGEIQWPFPQPLPV
                     GPLMNYGYEDEVVLLAELTPPSSAPAGNASIKARADWLVCKDICVPEKGELDFV"
BASE COUNT          277 a          503 c          546 g          254 t
ORIGIN      
        1 gccgcctccg aaaggaacgt cccggcgccg cacatcggat cgaggagcgg cacgccgggg
       61 cgccacttcg ccaggcgcag gattccagcg gcgagattct tcttgagggg agcctcgccc
      121 acgtggtcgc gcttgccgcg cttgaagagc gagtcgccgc tggtgtcgac gtagagcgtg
      181 cacatcttcg cgtcgaggaa cacatggatg cgcacgtcgg gatcgcgcga ttcgaccgag
      241 ggccgccttc cggcggctgc gcggaactgg tcggcgacgg cgtccttcac ccgcagcgtg
      301 atgaagtcga ggctcttcac cgggctgcgg tgcgcgttgg tatcgatctt gaacgtgcga
      361 tcggcgttga agtggctgtt ccagcgtatt cccgccgcgc cattgtagac gtcgtcttcg
      421 ctcttgtagg ggaactgcgc cacgcgccac aggatgcggc tcgcgatgcg cgaatgcagg
      481 ttcgcgatgt acacgagatc gaacgcgccg ctgaaggcga cgccggcttc ggcgggctcg
      541 atggcgcccg cgccgagctc ccccagctcc tcggcgagcg cgcccgaaag gccgcgcggg
      601 cacggggcga agaaataatg cgtgcccgtg tcctgtttaa ccaggatgcg cggcgcgggc
      661 ttcgcgtcgg tggtcatttc agaagggctt caccacgacg aggatgacga tggcggcaag
      721 cagcaccagg ggcagctcgt tgatccagcg gtagaagaca tggctgcggc gattcgcgtc
      781 ggccgcgaag tccatggcga ggcgccccag ccacaagtga tagcccacga ggaggaccac
      841 gaggaccagt ttcgcgcgga gccaaccgcc ggtgaagccg tagccgagcc ataaccaggt
      901 cccgagcacg agcgtgagcg ccatgcaggg cgtcatgatg ccgcgataga gcttgcgctc
      961 catcaccttg aagcgctcgt tggaagcggc atccgatgat agggcgtgat agacgaagag
     1021 gcgcggcagg tagaacaggc ccgcgaacca cgcgacgacg aagatgatgt gcagcgcttt
     1081 gagccagagc ataagactat aattttacgt gatgttcaca ctctctcccg ttctcgccgc
     1141 ggctttcgcc gcgattgccc tcctcggctc gcccgcttcg cgcgcggcga ccctggtgaa
     1201 gaccgagcac gtcgaggccg atctcgtcgc cgacaaggcg agcgcgcagc ccggcaagcc
     1261 gctgcgcgtg gggttgcgcc tgcgcatgga gccgcagtgg cacacgtact ggaggaaccc
     1321 cggcgactcg gggttgccga cgaagatcca gtggatcctt cccgagggct ggaaggcggg
     1381 cgagatccag tggccgttcc cgcagccgct gcccgtgggc ccgctcatga actacggcta
     1441 cgaggacgag gtggtgctcc tcgcggagct gacgccgccc tcctcggcgc cggcgggcaa
     1501 cgcctcgatc aaggcgcgcg ccgattggct cgtctgcaag gacatctgcg ttcccgagaa
     1561 gggcgagctc gatttcgtcc
//
LOCUS       DASVII010000268         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_49062, whole
            genome shotgun sequence.
ACCESSION   DASVII010000268 DASVII010000000
VERSION     DASVII010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49062"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VF309_03480"
     CDS             complement(<1..578)
                     /locus_tag="VF309_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7465678.1"
                     /translation="MKLKVLNPATGEVAALLDADDARTVKLKYREARTAQPAWARTPM
                     RKRLAAIAAFRERVVAEADSLAKILTTEVGKPISQSRNELKGVLPRIDFFLAETARTL
                     KPEKVFADAQGSMEERISHEPLGVVANISAWNYPWFVGANVFVPALLAGNAVLYKPSE
                     YASLTGLEIARLLHESGVPSDVFIPLIGAGPVG"
     gene            complement(575..1393)
                     /locus_tag="VF309_03485"
     CDS             complement(575..1393)
                     /locus_tag="VF309_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179871.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX7465679.1"
                     /translation="MGILKIGVSARLLYPDSSRTFLPTKSVQYLEQSVANWIMSGDVL
                     AFMIPEMSLASPHGPNHLKVKNYVDALDGLLLQGGADMSPASYGEQPINPLWAGDTVR
                     DAYEIDLFHEFVTQGKPVFGICRGHQVINVALGGKLYQDIATQCPDMGSHRDEAKYEN
                     HFHDMRILPNTWLARLYPQVETRRINSIHHQGVKTLGEGLVPEAVSEPDGIVEAIRWE
                     GHSFVVGVQWHPEFIDPKEPSLIDSRPLLRAFLAACESRKRTGKASPVMASKAA"
BASE COUNT          246 a          510 c          541 g          287 t
ORIGIN      
        1 cccaccgggc ccgcgccgat gagcgggatg aagacgtcgc tcggcactcc ggactcgtgc
       61 agtaggcgcg cgatctcgag gccggtgagc gacgcgtact ccgagggctt gtagagcacg
      121 gcgttgcctg cgaggagcgc gggaacgaac acgttcgccc ccacgaacca cgggtagttc
      181 cacgcggaaa tgttggcgac gacgccgagg ggctcgtggg agatcctttc ttccatcgag
      241 ccctgcgcgt cggcgaacac cttctcgggc ttgagcgtcc tcgcggtctc ggcgaggaag
      301 aagtcgatgc gcgggagcac tcccttcagc tcgttgcgcg attgcgagat gggcttgccg
      361 acttccgtgg tgaggatctt cgcgagggaa tcggcttcgg cgaccacgcg ctcgcgaaac
      421 gcggcgatgg cggcgaggcg cttcctcatc ggggtgcgtg cccacgcggg ctgtgcggtg
      481 cgggcttcgc gatacttcag cttgacggtg cgcgcgtcgt ccgcgtcgag gagcgccgcg
      541 acctcgcccg tcgcgggatt gaggaccttc agcttcacgc cgccttgctg gccatcacgg
      601 gcgacgcctt gccggtgcgc ttgcgcgact cgcacgcggc gaggaacgcg cgcaacagcg
      661 ggcggctgtc gatgagcgaa ggctccttcg gatcgatgaa ttccgggtgc cactgcacgc
      721 cgaccacgaa gctgtggcct tcccagcgga tcgcttccac gatgccgtcg ggctcgctca
      781 ccgcctcggg caccagccct tcgccgagcg tcttcacgcc ctggtggtgg atggagttga
      841 tgcgccgtgt ctcgacctgc gggtagaggc gcgcgagcca ggtgttgggc aggatgcgca
      901 tgtcgtggaa gtggttctcg tacttggcct cgtcgcgatg cgagcccatg tccgggcact
      961 gcgtcgcgat gtcctggtag agcttgccgc cgagcgcgac gttgatcacc tggtggccgc
     1021 ggcagatgcc gaacacgggc ttgccctgcg tcacgaattc gtggaagagg tcgatctcgt
     1081 aggcgtcgcg caccgtatcc cccgcccaca gcggattgat cggctgctcg ccatagctcg
     1141 cgggagacat gtccgcgccg ccctgcagga gcagcccgtc gagcgcatcg acgtagttct
     1201 tcaccttgag gtggttgggc ccgtgcggcg aggcgaggct catctcggga atcatgaacg
     1261 cgagcacgtc gcccgacatg atccagttgg cgaccgattg ctccaggtac tgcaccgact
     1321 tggtcggcag gaaggtgcgg ctcgagtcgg gatagagcag ccgcgccgag acgccgatct
     1381 tcaggattcc catcccaata ttatccccta gaacaggtgt cgcgtgatcg ccttggtggc
     1441 gctcacgccg aacgccgcct agagcgcctg cgtaaagatc cgctcgaaat ccgcagccga
     1501 cacgggcttc gggttggtct ggtggcatat gtcgttcacg gcgatctcca ccaggcgcgg
     1561 aatatcgctc gcccgcaccc ctct
//
LOCUS       DASVII010000269         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_336840,
            whole genome shotgun sequence.
ACCESSION   DASVII010000269 DASVII010000000
VERSION     DASVII010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336840"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VF309_03490"
     CDS             <1..264
                     /locus_tag="VF309_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX7465680.1"
                     /translation="ALGTPLLSLLGAACAALTLGARGAGMLLALLALPLFVPVLIFGA
                     GAAEAQSSGLSPSPHLSLLGAGLIVGAMVLPAAACAAIRIALD"
     gene            261..983
                     /gene="ccmC"
                     /locus_tag="VF309_03495"
     CDS             261..983
                     /gene="ccmC"
                     /locus_tag="VF309_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX7465681.1"
                     /translation="MSVNFFYFASPSTFYRLAGILAPWCFAVAAVLGAAGLYVGLVQA
                     PTDAQQGEVYRVIFIHVPAAWMSMFLYFVMACYGAMSLTFNTRLAAMMARALAPTGAM
                     FTFVALWTGAFWGRPTWGAYWVWDARLTSELVLLFLYFGFIALGNAFEDPRRGDRAAA
                     LLALIGVVNLPIIYFSVLWWNTLHQGSSVNFKGTSMHVSMLTGMLLMAFSFWSYSIGT
                     ALARVRTIILERERRTRWVRAL"
     gene            965..1120
                     /gene="ccmD"
                     /locus_tag="VF309_03500"
     CDS             965..1120
                     /gene="ccmD"
                     /locus_tag="VF309_03500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX7465682.1"
                     /translation="MGARLMNHDFYIWMSYGMTALAIAVEIAWLRLHRAQALRRIEEE
                     RDLEAQD"
     gene            1104..1571
                     /gene="ccmE"
                     /locus_tag="VF309_03505"
     CDS             1104..1571
                     /gene="ccmE"
                     /locus_tag="VF309_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX7465683.1"
                     /translation="MKPRTRRAIWIVSGLAALGIASALVLNAFQSNLVFFFTPSQVAA
                     HEAPSGRAFRIGGLVEAGSVVHEKDALTVHFRVTDMAQTIPVVFTGHLPDLFREGKGV
                     VAQGKLLGDGTFHASDVLAKHDENYMPPEAAEALRKAGHPIDKAAFAGQKGGK"
     gene            1568..>1782
                     /locus_tag="VF309_03510"
     CDS             1568..>1782
                     /locus_tag="VF309_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmF"
                     /protein_id="HEX7465684.1"
                     /translation="MIAELGHFSLVVALALAVAQVGLSFAGARTGRASWLATARPIAV
                     GQFVFVAGAFAALAYSFVSNDFSVLNVA"
BASE COUNT          224 a          600 c          606 g          352 t
ORIGIN      
        1 gccctgggca cgccgctgct gtcgctgctg ggagccgcat gcgcggccct cacgctaggc
       61 gcgcgcggag cgggcatgct gctcgcactc ctggcgctgc ccctgttcgt gccggtgctc
      121 atcttcgggg cgggcgcggc cgaggcgcag tcttcggggc tttcgccctc tccacacctc
      181 tcgcttctcg gcgcgggact catcgtcggc gcgatggtgc tgcccgcggc ggcgtgcgcc
      241 gcgattcgca tcgcactgga ctgagcgtga acttcttcta cttcgcctcg ccctcgacct
      301 tctaccgcct cgcgggaatc ctcgcgccgt ggtgcttcgc ggtcgcggcg gttctcggcg
      361 ccgcgggcct ctacgtcggc ctcgtccagg cgccgaccga cgcgcagcag ggcgaggttt
      421 atcgcgtcat cttcatccac gttcccgccg cgtggatgtc gatgttcctc tacttcgtga
      481 tggcctgcta cggcgccatg tcgctcactt tcaacacgcg gctcgccgcg atgatggcgc
      541 gcgcgctcgc gcccaccggg gcgatgttca ccttcgtcgc cctgtggacg ggcgccttct
      601 ggggccgccc gacgtggggc gcctattggg tgtgggatgc ccggctcacc tcggagctgg
      661 tgctgctctt cctctacttc gggttcatcg cgctggggaa cgcgttcgaa gacccgcgcc
      721 gcggcgaccg cgccgcggcg ctgctcgcgt tgatcggagt cgtgaacctg cccatcatct
      781 acttctcggt gctatggtgg aatacgcttc accagggatc gagcgtgaac ttcaagggca
      841 cgtcgatgca cgtctcgatg ctcaccggca tgctgctgat ggctttctcg ttctggagct
      901 attcgatcgg gaccgcgctt gcgagggtcc ggacgatcat cctcgagcgg gagcgccgga
      961 cgcgatgggt gcgcgcctta tgaatcatga tttctacatc tggatgtctt acggcatgac
     1021 ggcgctggcg atcgcggtgg agatcgcgtg gctgcgcctg catcgcgcgc aagcgctgcg
     1081 ccgcatcgaa gaggagcgtg accttgaagc ccaggactag gcgggcgatc tggatcgtgt
     1141 cgggactcgc cgcgttgggg atcgcgtcgg cgctggtgct gaacgcgttc cagtcgaacc
     1201 tcgtattctt tttcactccg agccaggtgg cggcccacga ggctccgtcc ggacgggcct
     1261 ttcgcatcgg cggcctcgtc gaggccggga gcgtcgtgca cgagaaggac gcgcttaccg
     1321 tccatttccg cgtgaccgac atggcgcaaa ccatcccggt cgtgttcacg ggccatctac
     1381 cggatctctt ccgcgaggga aaaggcgtcg tggcgcaggg caagctcctg ggcgacggca
     1441 ccttccacgc gagcgatgtt ctcgcgaagc acgacgagaa ctacatgccg cccgaagcgg
     1501 cggaggcgct gcgcaaggcg ggccatccca tcgacaaggc cgccttcgcg ggccagaagg
     1561 gcggcaaatg atcgcggagc tcgggcattt ctcgctggtg gtggcgctgg cgctagcggt
     1621 ggcgcaggtg ggtctctcct tcgcgggcgc gcgcaccggg cgcgcctcgt ggctcgccac
     1681 cgcgcgcccg atcgccgtcg ggcagttcgt gttcgtcgcg ggcgccttcg ccgcgctcgc
     1741 gtattcgttc gtctcgaacg acttctcggt cctgaacgtg gc
//
LOCUS       DASVII010000270         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_336859,
            whole genome shotgun sequence.
ACCESSION   DASVII010000270 DASVII010000000
VERSION     DASVII010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336859"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VF309_03515"
     CDS             <1..862
                     /locus_tag="VF309_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7465685.1"
                     /translation="LVRGSTPLAIDVNFVDDCAEATVLGDPTRMHQLLMNLCTNAIHS
                     MGNGGTLELRVACEEFTEPRKVRSGEIAPGEYVRISVKDTGHGIAPEVIDRIFEPFFT
                     TKPAGRGTGLGLALVHSVVTEHKGFIEVASELGRGTTFSVWMPQIHAEEGAGDELASL
                     PMGGGQVILAVDDELDVLHALEEMLAQLGYEPVGFGNSREALEAMRANPRRFDAVVSD
                     EVMPELIGTQLANELRRINPTLPIVIASGYGGAGFEARALSAGVNRVLRKPYRMSEIA
                     EALAIFFAKS"
     gene            973..>1654
                     /locus_tag="VF309_03520"
     CDS             973..>1654
                     /locus_tag="VF309_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7465686.1"
                     /translation="MREGMHAAAEELVRASGRANLDWEGASLTTHFQPIYCVRRESSI
                     GFEALVRARDEHGAMIAVEDLFARTADSSRSLLDWACRAMHLRNYATVDPGDRTLFVN
                     IHPEAAVRDAGRTREFAELVRYYGLTPKRVCIEILEAPCSSEKLLRDAVEGYRDLGAT
                     IAMDDFGVGCSNFDRVTSLRPDVVKIDRSILAGAAGKDKARRMLPVMIELLHEFHAKV
                     AVEGIETQA"
BASE COUNT          281 a          564 c          571 g          238 t
ORIGIN      
        1 cctggtgcgc ggctcgacgc cgctcgcgat cgacgtgaac ttcgtcgacg actgcgccga
       61 ggccaccgtg ctgggcgacc ccacgcgcat gcaccagctc ctcatgaacc tctgcaccaa
      121 cgcgatccat tccatgggca atggcggcac gctcgagctg cgcgtcgcgt gcgaggagtt
      181 caccgagccg cgcaaggtga ggagcggcga gatcgcgccg ggcgaatacg tgcgcatctc
      241 ggtgaaggac accggccacg gcatcgcgcc ggaagtgatc gaccgcatct tcgagccgtt
      301 cttcaccacc aagcccgcgg ggcgcggcac cggcctcggg cttgcgctgg tgcattccgt
      361 ggtgaccgag cacaagggct tcatcgaggt cgcgagcgag ctgggacgcg gcaccacctt
      421 cagcgtgtgg atgccgcaga tccacgccga ggagggcgcc ggcgacgagc tcgcctcgct
      481 gccgatgggc ggggggcagg tgatcctcgc cgtggacgac gagctcgacg tgctgcacgc
      541 gctggaggag atgctcgcgc agctgggcta cgagccggtg ggcttcggca acagccgcga
      601 agcgctcgag gcgatgcgcg cgaacccgcg gcgcttcgac gccgtggtga gcgacgaggt
      661 gatgccggag ttgatcggca cccagctcgc caacgagctg cgccgcatca atccgacgct
      721 ccccatcgtc atcgcgagcg gctacggcgg cgcgggcttc gaggcgcgcg cgttgtccgc
      781 gggcgtgaac cgcgtgctgc gcaagcccta tcgcatgagc gagatcgccg aggcgctggc
      841 gatcttcttc gccaagtcgt gactacaaac gcaaccatcc ggggcggcac cgtaaccccg
      901 ctgtaacccg ccgccgccaa agtggcatga agccgggaat cgtccggcct cgaccggggt
      961 ggggaaacaa tcatgaggga aggaatgcac gccgccgctg aagagctggt gcgcgcaagt
     1021 ggccgtgcga acctcgattg ggagggcgcg agcctcacga cccatttcca gccgatctat
     1081 tgcgtgcgcc gcgagtccag catcggcttc gaggccctgg tgcgcgccag ggacgagcac
     1141 ggcgccatga tcgccgtgga ggatctcttc gcgcgcactg ccgattccag ccgctcgctc
     1201 ctcgactggg catgccgcgc gatgcacctg cgcaactacg ccaccgtcga tccgggcgac
     1261 cgcacgctct tcgtcaacat ccacccggaa gcggcggtgc gcgacgcggg ccgcacgcgc
     1321 gaattcgccg agctggtgcg ctactacggc ctcacgccca agcgcgtctg catcgagatc
     1381 ctcgaagcgc cgtgctccag cgagaagctg ctgcgcgacg cggtcgaagg ctaccgcgac
     1441 ctgggagcca cgatcgccat ggacgacttc ggcgtgggct gctccaattt cgaccgcgtg
     1501 acgagcctgc gccccgatgt ggtgaagatc gaccgctcga tcctcgccgg cgccgcgggc
     1561 aaggacaagg cgcggcgcat gctgccggtg atgatcgagc tgctgcacga attccacgcg
     1621 aaggtcgcgg tggaaggcat cgagacccag gccg
//
LOCUS       DASVII010000271         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_264733,
            whole genome shotgun sequence.
ACCESSION   DASVII010000271 DASVII010000000
VERSION     DASVII010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264733"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF309_03525"
     CDS             complement(<1..577)
                     /locus_tag="VF309_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7465687.1"
                     /translation="MEFPTALVLNAIIAGLLLGGFYAAVSVGVSISFGMLDVVNIAHP
                     AFVILGSYIAYIVNDRLGLDPIAVSIVLSPLFFLLGMALYRIYYLCFEKRGQESLRGL
                     AFFFGILFITEVALVLIFGVDYRMARTAYADTTWRLGPVDFPMRLVVPFLVSVTMVVG
                     LQLYFSHSFFGRAVLAVAQDALALRLMGVDTF"
     gene            complement(628..1878)
                     /locus_tag="VF309_03530"
     CDS             complement(628..1878)
                     /locus_tag="VF309_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7465688.1"
                     /translation="MKRVTAWIKGGVATVLVLLAIPVLAQQPLKIGFGMSLTGPLAGN
                     GKAALIAMDIWKDDVNAKGGILGRKVEFVYYDDQTNPSTVPSIYSKLLDVDKVDLVVS
                     GYGTNVIAPAMPIMMQRNMTFMGLFGLNVNSKFDYDRYFQIMPAGPDPAIGWTQGYFD
                     TAMSLNPKPQTIALVGADAEYPGLALDGARAQVKRTGLKIVYDKSYPPNTVDFSPIIR
                     AIQATNPDLVFVASYPPDSAGMIRAANEVGLKTRMFGGGMVGPQFAALKTQLGPLLNG
                     LVNYESYAPDVADKFPSIDQFLVKYQARAAKEGVDVLGFYLPPFAYAMMEVLEQSIKA
                     VGSLDQAKLADYMHKAEFSTYVGKVKFAKNGEWAQPRMLTVQYHGITGNDLDQWRKPG
                     HVTVLTPAPYKTGSVMTPYQDSRK"
     gene            2042..3721
                     /locus_tag="VF309_03535"
     CDS             2042..3721
                     /locus_tag="VF309_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7465689.1"
                     /translation="MNLQSFRINTRLLVGFAIVLILVGAGSLVGVWQLKSLEGVVERL
                     ATEEIAKVTLAELWISTIATNVVRLEAALALDPKDEIAERLRRDMDGTVKQVAERDKA
                     LETLVTAPEGKRLMENISVIRKRYKDYKAQMIERRAKGEDVREDLKTTFAPLARDYLA
                     AIQLFVDWERAQLDDARKNAAAAAHSARTAIMITAACGLLGGLAIALVLSRSIVAPIN
                     RARDSARLIASGDLNGAIELSGHDEAAAMLRDLAEMQKRLRDIVDKVRLGAEAVTVTS
                     GQIAAGNADLSSRTEQQASSIEETAASLEELASTVDQNAENAKKADKLAHSASQVADR
                     GGAVVGKVVSTMDDIRKSSRKISEIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFA
                     VVAAEVRSLAQRSAAAAKEIKGLITDSAKTVDSGSRLADEAGATMQEVVESVQRVSEV
                     ISQIAVATQEQNAGIGQVNTAVTELDRVTQQNAALVQESAAASESLKEQAARLTQSVA
                     AFKHDGRRAPPQPELSRQVFATARRATAPAASRALEVIAAAKDKDEEWKEF"
BASE COUNT          691 a         1178 c         1264 g          610 t
ORIGIN      
        1 gaaacgtatc gacgcccatg aggcgcaggg cgagcgcgtc ctgcgccacg gcgagcaccg
       61 cgcgcccgaa gaaggagtgg gagaaataga gctgcaggcc gaccaccatc gtgaccgaca
      121 ccaggaaggg gaccaccagc cgcatcggga agtcgaccgg gccgaggcgc cacgtcgtgt
      181 ccgcgtaggc ggtgcgcgcc atgcggtagt cgacgccgaa gatgagcacg agggcgacct
      241 cggtgatgaa gaggatgccg aagaagaagg cgaggccgcg cagcgactcc tgcccgcgct
      301 tctcgaagca aaggtagtag atgcgataga gcgccatccc cagcaggaag aagagcggcg
      361 agagaacgat ggagaccgcg atgggatcga ggcccagccg gtcgttcacg atgtaggcga
      421 tgtacgagcc gaggatgacg aacgccgggt gcgcgatgtt gaccacgtcc agcatgccga
      481 acgagatcga aacgcccacg ctgacggcgg cgtagaagcc gccgagcaaa agcccggcga
      541 tgatggcgtt gagcaccaac gccgtcggga attccattat tcggtgtcta cgctagcgaa
      601 ggcgtgtccc ttgtttcgcg ccgctcccta tttcctgctg tcctggtacg gcgtcatcac
      661 gctgcccgtc ttgtagggcg cgggcgtgag caccgtcacg tggccaggct tgcgccactg
      721 gtcgaggtcg ttgccggtga tgccgtggta ctggaccgtg agcatgcgcg gctgggccca
      781 ctcgccgttc ttggcgaact tcaccttgcc cacataggtg ctgaattcgg ccttgtgcat
      841 gtagtcggcg agcttggcct ggtcgaggct tcccaccgcc ttgatcgact gctcgagcac
      901 ttccatcatc gcgtaggcga agggcggcag gtagaagccg aggacgtcga cgccttcctt
      961 cgccgcccgc gcctggtact tcaccaggaa ctggtcgatg gaaggaaact tgtcggcgac
     1021 atcgggcgcg tagctctcgt agttgacgag gccgttgagc agcgggccga gttgcgtctt
     1081 gagtgctgcg aactgcgggc ccaccatgcc gccgccgaac atgcgcgtct tcaggccgac
     1141 ctcgttcgcc gcgcggatca tgccggcgga atcgggcgga taggacgcga cgaagaccag
     1201 gtccggattg gtcgcctgga tcgcgcggat gatcggcgag aagtccacgg tgttgggcgg
     1261 gtagctcttg tcgtagacga tcttcagccc ggtgcgcttc acctgcgcgc gagccccgtc
     1321 gagagcgagg ccgggatact cggcatcggc gcccacgagg gcgatcgtct gcggcttggg
     1381 attcaggctc atcgccgtgt cgaaatagcc ctgcgtccag ccgatcgccg gatccgggcc
     1441 cgctggcatg atctggaagt agcggtcgta gtcgaacttg gagttcacgt tcaggccgaa
     1501 gaggcccatg aaggtcatgt tccgctgcat catgatcggc atcgcgggcg cgatcacgtt
     1561 ggtgccgtag cccgacacca cgaggtccac cttgtcgacg tcgaggagct tcgaatagat
     1621 gctcggcacc gtcgagggat tcgtctggtc gtcgtagtag acgaattcca ccttgcgccc
     1681 gaggatcccg cccttcgcat tgacgtcgtc cttccagatg tccatcgcga tgagcgccgc
     1741 cttgccgttg cccgcgaggg gcccggtgag gctcatgccg aagccgatct tgaggggctg
     1801 ctgcgcgagg accggaatcg cgagcaacac caggacggta gcgaccccgc ccttgatcca
     1861 cgccgtcacc cttttcattt gctgctcccc ccttttttat tcgtggcttg gcgatgctac
     1921 accagccaca ttgcgtacgt aacttcgccg tagcttcggg gtagctcgca tctgtcacac
     1981 tccgttcagg catccgctgc cttttcgccg gagccaagag acgttacaga ggagttcggc
     2041 aatgaacctg cagtctttcc gcatcaacac gcgcctgctc gtgggtttcg ccatcgtgct
     2101 catcctcgtg ggcgccggtt cgctggtggg ggtctggcag ctgaagagcc tcgaaggggt
     2161 tgtcgaacgc ctggccacgg aggaaatcgc caaggtcacg cttgccgagc tctggataag
     2221 caccatcgca accaacgtcg tgcgcctcga ggcggcgctc gcgctcgatc ccaaagacga
     2281 gattgccgag cgcctgcgca gggacatgga cggcaccgtg aagcaggtcg ccgagcgcga
     2341 caaggcgctg gagacccttg tcacggcccc ggaaggcaag aggctcatgg aaaacatttc
     2401 cgtgatccgc aagcgctaca aggattacaa ggcgcagatg atcgagcgcc gcgcgaaggg
     2461 cgaggacgtc cgggaggacc tgaagacgac attcgccccg ctcgccaggg actacctcgc
     2521 cgcgatccag ctgttcgtgg attgggagcg ggcgcagctc gacgacgcgc gcaagaacgc
     2581 cgccgccgcc gcgcactccg cgcgcaccgc catcatgatc accgcggcgt gcggcctgct
     2641 gggcggcctc gcgatcgcgc ttgtgttatc gcgttccatc gtggcgccca tcaatcgcgc
     2701 ccgggacagc gcccgcctca tcgcatcggg ggacctgaat ggcgccatcg agctgtccgg
     2761 ccatgacgag gccgccgcga tgttgcgcga cctcgcggaa atgcaaaaga ggctgcggga
     2821 catcgtggac aaggtgcgcc tgggcgccga agcggtgacg gtgacctcgg ggcagatcgc
     2881 cgccggcaat gcggacctgt cgtcgcgcac cgagcagcag gcttccagca tcgaggagac
     2941 cgccgcgtca ctggaggagc tcgcctcgac ggtcgaccag aacgccgaga atgcgaaaaa
     3001 ggccgacaag ctcgcccatt ccgcctccca ggtcgccgat cgcggcggcg cggtggtggg
     3061 caaggtggtg agcaccatgg acgacatccg caagagcagc aggaagattt cggagatcat
     3121 cggcgtgatc gacggcatcg cgttccagac caacatcctc gcgctcaacg ccgccgtcga
     3181 agcggcgcgc gcaggcgagc aggggcgcgg cttcgccgtg gtcgcggcgg aggtgcgcag
     3241 cctcgcccag cgctcggccg cggcggccaa ggagatcaag ggactcatca ccgactcggc
     3301 gaagaccgtg gattcgggct cgaggctcgc cgacgaggcc ggcgcgacca tgcaggaagt
     3361 ggtcgaaagc gtgcagcggg tctccgaggt catcagccag atcgcggtgg ccacgcagga
     3421 gcagaacgcg ggcattggcc aggtcaacac cgcggtcacc gagctcgatc gcgtgacgca
     3481 gcagaatgcc gcgctggtgc aggaatcggc ggccgcgtcc gagagcctca aggagcaggc
     3541 tgcgcgcctc acccaaagcg ttgcggcgtt caagcacgac ggtcgccgcg cgcccccgca
     3601 gcctgagctt tcgcggcagg tctttgccac ggcgcgtcgc gctactgcac cggcggccag
     3661 ccgggcgctg gaagtgatcg cggccgcgaa ggacaaggat gaagaatgga aggagttcta
     3721 gtccactatt ccgtcttcat gtt
//
LOCUS       DASVII010000272         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_228995,
            whole genome shotgun sequence.
ACCESSION   DASVII010000272 DASVII010000000
VERSION     DASVII010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228995"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..464)
                     /locus_tag="VF309_03540"
     CDS             complement(39..464)
                     /locus_tag="VF309_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7465690.1"
                     /translation="MAMNVGSGAGGDPEVMVDINTTPLIDVMLVLLIMLIITIPIQTH
                     AVKLNMPVGNPPPPLEPPVVVTIEVDFDGTILWNGDQVDRPTLDAKLTNAAAMPVQPE
                     FHLRPNKLVAYKYVAHVMAAAQRLGVTKIGLIGAEQFLD"
     gene            complement(484..927)
                     /locus_tag="VF309_03545"
     CDS             complement(484..927)
                     /locus_tag="VF309_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7465691.1"
                     /translation="MAMTVGQDVAEGEQEVMAAINTTPLVDVMLVLLIIFLITIPAIV
                     QQVPLVLPKERNVPTQTKPENIVIAINKDGDVFWGMEYLKHSKALVERLKKVAVMVPQ
                     PEVHIRADGDARYESVGKVVATCQRASILKIGFITEPPERALAGG"
     gene            complement(935..>1616)
                     /locus_tag="VF309_03550"
     CDS             complement(935..>1616)
                     /locus_tag="VF309_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7465692.1"
                     /translation="LEALWRGGDFVSRGTLIILIIMSMGSWYIMFVKVYEQAKIFGQY
                     RQVQAKFWNAGSIPEGIQELKDGTPYRYIAESGIKSTQHHEGKLLEQIDLNTWVTMSL
                     QRAVENVSSRLQDGLAFLATVGSTSPFVGLFGTVWGIYHALTAIGIAGQASIDKVAGP
                     VGEALIMTAFGLAVAVPAVLGYNWLVRRNKVANEQLRAFGADLHMVLVSGGGSAKRAP
                     APATAAAR"
BASE COUNT          254 a          524 c          538 g          300 t
ORIGIN      
        1 gcaccgcgtg cgggcgtccg aatttcatcg cgtatctcct agtccaggaa ctgctcggcg
       61 ccgatcaatc cgatcttcgt gacgccgagg cgctgcgccg cggccatcac gtgcgcgacg
      121 tacttgtacg cgaccagctt gttcgggcgg aggtggaact cgggctgcac cggcatcgcc
      181 gcggcgttcg tgagctttgc gtcgagcgtg ggacggtcga cctggtcgcc gttccacagg
      241 atcgtgccgt cgaagtccac ctcgatcgtg accaccaccg gcggctccag cgggggcggc
      301 ggattgccca ccggcatgtt gagcttcacg gcgtgcgtct ggatcgggat cgtgatgatc
      361 agcatgataa ggagcaccag catcacgtcg atcaagggcg tcgtgttgat gtcgaccatg
      421 acctcgggat cgccgcctgc gccactgcct acgttcattg ccattgcgtg tttcctcgtt
      481 cgcctagccg cccgcgaggg cgcgctcggg aggctcggtg atgaagccga tcttgaggat
      541 cgacgcgcgc tggcaggtgg ccaccacctt gccgaccgac tcgtagcgcg catcgccgtc
      601 ggcgcggatg tggacctcgg gctgcggcac catcaccgcg accttcttca gcctctcgac
      661 gagcgccttg gaatgcttca ggtattccat gccccagaag acgtcgccat ccttgttgat
      721 cgcgatgacg atgttctcgg gcttggtctg cgtcgggacg ttgcgctcct tcggcagcac
      781 cagcggcacc tgctgcacga tcgccgggat cgtgatgagg aagatgatga gcagcaccag
      841 catcacgtcg accagcggcg tggtgttgat ggcggccatg acctcctgtt cgccctcggc
      901 gacgtcctgg ccaacggtca ttgccatgag cgatttacct tgccgcggcc gtcgcgggcg
      961 ccggcgcgcg cttcgcgctg ccgccgccgc tcacgagcac catgtgcaga tccgcgccga
     1021 acgcgcgcag ctgctcgttg gcgaccttgt tgcggcgcac gagccagttg tagccgagca
     1081 ccgccggcac cgcgaccgcg aggccgaagg ccgtcatgat gagcgcttcg cccacgggac
     1141 ccgcgacctt gtcgatcgag gcctgtcctg cgatgccgat ggcggtcagc gcgtggtaga
     1201 tgccccacac cgtgccgaag aggccgacga agggagaggt cgagccgacc gtcgcgagga
     1261 aggcgaggcc gtcctggagg cgcgaggaga cgttctcgac ggcgcgctgc agcgacatcg
     1321 tgacccaggt gttcaggtcg atctgctcga ggagcttgcc ctcgtggtgc tgcgtggact
     1381 tgatgccgct ctcggcgatg tagcgatagg gcgtgccgtc cttgagctcc tggatgcctt
     1441 cggggatcga gcccgcgttc cagaacttgg cctgcacctg gcggtactgg ccgaagatct
     1501 tggcctgctc gtagaccttc acgaacatga tgtaccacga gcccatcgac atgatgatga
     1561 ggatgatgag cgtgccgcgc gacacgaagt cgccgcccct ccacagggcc tcgagg
//
LOCUS       DASVII010000273         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_229016,
            whole genome shotgun sequence.
ACCESSION   DASVII010000273 DASVII010000000
VERSION     DASVII010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229016"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VF309_03555"
     CDS             complement(<1..153)
                     /locus_tag="VF309_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7465693.1"
                     /translation="MTYVVTESCVKCKYTDCVDVCPVDCFREGPNFLVIDPDECIDCT
                     LCVAECP"
     gene            340..669
                     /gene="trxA"
                     /locus_tag="VF309_03560"
     CDS             340..669
                     /gene="trxA"
                     /locus_tag="VF309_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX7465694.1"
                     /translation="MAEKSIVHITDDTFESEVLQSQTPVLVDYWAEWCGPCKMIAPAL
                     DQIAAEYTGRLKVAKLNIDENQSTPPKYGIRGIPTLMLFKNGAVEATKVGALSKTQLA
                     AFIDSNI"
     gene            840..>1741
                     /gene="rho"
                     /locus_tag="VF309_03565"
     CDS             840..>1741
                     /gene="rho"
                     /locus_tag="VF309_03565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7465695.1"
                     /translation="MHLSDLKLKPITELVEMAAVNELEGANRLRKQELVFALLKHQAK
                     KGEAIFGDGTLEVLQDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIIGEIR
                     TPKDGERYFALVKVDTVNGEPPENAKTKILFENLTPLHPDEPMRLERDIKAEENLTGR
                     ILDIIAPIGKGQRGLIVSPPKAGKTVLMQHMAHAITANHPEAVLIVLLIDERPEEVTE
                     MSRTVRGEVVASTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDSNALQRPKRF"
BASE COUNT          351 a          597 c          515 g          278 t
ORIGIN      
        1 cggacactcg gcgacacaca gcgtgcagtc gatgcattcg tcgggatcga tgacgaggaa
       61 attgggacct tcgcgaaagc agtcgaccgg gcacacgtcg acgcagtcgg tgtacttgca
      121 cttcacgcag gactcggtca ccacgtacgt catgaaaggc ctttcctgag gctgcggcgc
      181 gggggcgaaa ggcggccatt ttatcagacg ccccgcatcc catcccaacc cggcgctttg
      241 cccggaacgc ccgttttggt ctacgattgc aaccatagcg ccgccccccg gttcccacga
      301 acccccttcc ccgggcgaga cgaatccaag cgaggaaaca tggctgagaa atcgatcgtt
      361 cacatcacag acgacacgtt cgagtccgag gtgctgcagt cgcagacccc ggtgctcgtg
      421 gactactggg cggaatggtg cggcccgtgc aagatgatcg cgcccgcgct ggaccagatc
      481 gcggcggagt acacgggccg gctcaaggtc gcgaagctga acatcgacga gaaccagtcg
      541 acgccgccga agtacgggat ccgcggcatc ccgacgctca tgctcttcaa gaacggcgcg
      601 gtcgaggcga ccaaggtcgg cgcgctatcg aaaacccagc tcgcggcatt cattgacagc
      661 aatatctgaa cccgataacc tgaatcaatc ctggctgtcc gctcgcgccc cgtttgcaat
      721 aacgcccgcg tccagctttc ttcccgcggc caggtccttc ccaacatttc ttcgtttcac
      781 cggcccgtga ggtcttccgg gcccgttcac caaaactccc tccaggcagg ttccgcggca
      841 tgcacttatc cgacctcaag ctgaagccca tcaccgagct cgtcgagatg gcggccgtca
      901 acgagctcga aggcgccaac cgcctgcgca agcaggagct cgtcttcgcg ctcctcaagc
      961 accaggcgaa gaagggcgag gccatcttcg gcgacggcac cctcgaagtc ctccaggacg
     1021 gcttcgggtt cctgcgctcg ccggacacgt cctatctcgc cgggcccgac gacatctacg
     1081 tgtcgccctc gcagatcagg cgcttcaacc tgcatacggg cgattccatc attggcgaga
     1141 tccgcacgcc gaaggatggc gagcgctact tcgcgttggt gaaggtcgac acggtcaacg
     1201 gcgagccgcc cgagaacgcg aagaccaaga tcctcttcga gaacctcacg ccgctgcacc
     1261 ccgacgagcc gatgcggctc gagcgcgaca tcaaggccga ggaaaacctg accggccgca
     1321 tcctcgacat catcgcgccc atcggcaagg gccagcgcgg cctgatcgtc tcgcccccca
     1381 aggcgggcaa gacggtcctg atgcagcaca tggcccacgc catcaccgcg aaccatccgg
     1441 aagcggtgct gatcgtgctc ctcatcgacg agcgtcccga ggaagtgacc gagatgagcc
     1501 gcacggtgcg cggcgaagtc gtggcgtcca ccttcgacga accggcgacg cgccatgtgc
     1561 aggtcgccga gatggtgatc gagaaggcga agcgcctggt ggagcacaag aaggacgtgg
     1621 tgatcctgct cgattcgatc acgcgcctcg cacgcgccta caacaccgtg gtgcccgcct
     1681 cgggcaaggt gctcacgggc ggcgtcgatt ccaacgccct gcagcgcccg aagcgcttct
     1741 t
//
LOCUS       DASVII010000274         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_192620,
            whole genome shotgun sequence.
ACCESSION   DASVII010000274 DASVII010000000
VERSION     DASVII010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192620"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VF309_03570"
     CDS             <1..252
                     /locus_tag="VF309_03570"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7465696.1"
                     /translation="PLSTFVDVIRIAFLGYCVWLTLSLMQKIGSQRMAIIDWPIGIVY
                     GFVAFGFALMTWRAVGVARDNWKRGYSVLEVPELADEMR"
     gene            255..1661
                     /locus_tag="VF309_03575"
     CDS             255..1661
                     /locus_tag="VF309_03575"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7465697.1"
                     /translation="MGPGSFKMLFLGLMASGIPVAMAMAGASLVYVMATGNIPDYVII
                     HRMVGGLDSFPLLAVPFFILAGNLMNSAGITNRIYNFALALVGWMKGGLGHVNVVGSV
                     IFAGMSGTAIADAAGLGTIEIKAMQDHGYSTEFSVGVTAASATLGPIIPPSLPFVIYA
                     LFANVSVGALFLAGILPGAVMAILMMLTVAFYAHKNKWGADVPFQWPRVLKALTELLI
                     VLGWPTLVWIGVDRFDLDAQWTVLLGIVLLFVADWRFKFAAVLPIMTPVLLIGGMTTG
                     VFTATEGAIAACVWALFLGLAWYRTLSWKMLVKASMDTIETTSAVLFIVAAASIFGWM
                     LTATRTTEMIAAWVLSVTSTPAGFLLLANLLMLFVGCFLEPTAAITILVPILLPIVRK
                     LGIDPVHFGLVMVLNLMIGLLHPPMGLVLFVLARVAKLSVERTTMAILPWLVPLLISL
                     VIVTYIPSISLWLPNQFK"
     gene            1672..>2638
                     /locus_tag="VF309_03580"
     CDS             1672..>2638
                     /locus_tag="VF309_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX7465698.1"
                     /translation="MLRETPLYLRLNPADDVVIACRELERGTNLLQENVVCLERIPAG
                     HKVATRAVAKGEPVRRYSQIIGFASQPIAAGAHVHVHNLEVRDFARDYAFGEAYRPTQ
                     FASEPAMFKGIVRPDGRVATRNYVGILSTVNCSATVAKHVAKAFEGEALASYPNVDGV
                     VALTHSAGCGMDASGEGMDVLRRTLAGYARHANFAGVLVIGLGCEGNQMDALFFTQGL
                     AEGPLLRAMTIQDKGGTAKAVAEGIAIVKEMLPVANQVERSPVPASAIVVGLQCGGSD
                     GYSGITANPALGAAVDLLVRHGGGAILSETPEIYGAEHLLTRRAVT"
BASE COUNT          376 a          895 c          880 g          487 t
ORIGIN      
        1 ccgctgtcca ctttcgtcga cgtgatccgc atcgccttcc tcgggtactg cgtgtggctc
       61 accctctccc tgatgcagaa gatcggctcc cagcgaatgg cgatcatcga ctggcccatc
      121 ggcatcgtgt acggcttcgt cgcgttcggc ttcgcgctga tgacgtggcg cgcggtcggg
      181 gtcgcgcggg acaactggaa gcgcggctac tcggtgctcg aagtgcccga gctcgccgac
      241 gagatgaggt gatcatggga cccggaagct tcaagatgct tttcctgggc ctcatggcct
      301 cgggaattcc cgtcgcgatg gcgatggcgg gcgcctccct cgtctacgtg atggcgacgg
      361 gcaacattcc cgactacgtg atcatccatc gcatggtggg cgggctcgac tcctttccgc
      421 tcctcgccgt gccgttcttc atcctcgccg gcaacctcat gaattccgcg gggatcacca
      481 accgcatcta caacttcgcg ctcgcgctgg tgggatggat gaagggcggc ctgggccacg
      541 tgaacgtggt gggctcggtg atcttcgcgg ggatgtcggg cacggcgatc gcggacgcgg
      601 cgggcctggg cacgatcgag atcaaggcga tgcaggacca cggctactcg acggagttct
      661 ccgtcggcgt gaccgccgcc tcggccaccc tcggcccgat catcccgccg tcgcttccct
      721 tcgtgatcta cgcgctcttc gcgaacgtgt ccgtgggcgc gctcttcctc gccggcatcc
      781 tgccgggcgc ggtgatggcg atcctcatga tgctcacggt cgccttctac gcgcacaaga
      841 acaagtgggg cgccgacgtg ccgttccagt ggccgcgggt gctgaaggcg ctcaccgagc
      901 ttctcatcgt cctcggctgg cccacgctcg tctggatcgg cgtggaccgg ttcgatcttg
      961 acgcgcagtg gacggtgctc ctgggtatcg tgcttctctt cgtcgccgac tggaggttca
     1021 agttcgcggc ggtgctgccg atcatgacgc cggtgctcct catcggcggc atgacgaccg
     1081 gcgtcttcac cgcgaccgaa ggcgcgatcg ccgcgtgcgt gtgggcgctc ttcctcgggc
     1141 tcgcgtggta ccgcacgctc agctggaaga tgctggtgaa ggcctcgatg gacacgatcg
     1201 agacgacttc cgcggtgctc ttcatcgtcg ccgccgcgtc gatcttcggc tggatgctca
     1261 ccgcgacgcg caccaccgag atgatcgccg cgtgggtgct ctcggtcaca tcgacgcccg
     1321 cggggttcct gctgctcgcg aacctcctga tgctcttcgt cggctgcttc ctcgagccca
     1381 ccgcggcgat cacgatcctc gtgccgatcc tgctgccgat cgtgcgcaag ctcggcatcg
     1441 accccgttca cttcgggctg gtgatggtgc tgaacctgat gatcggcctg ctgcacccgc
     1501 cgatgggcct ggtgctcttc gtcctcgcga gggtcgccaa gctctcggtc gagcgcacca
     1561 ccatggcgat cctgccgtgg ctcgtgccgc tgctgatcag cctcgtgata gtgacgtata
     1621 ttccctcgat cagcctgtgg ctgccgaacc agttcaagta ggaattccgc catgttgaga
     1681 gagacaccgc tctacctgcg cctgaacccc gccgacgatg tcgtcatcgc gtgccgcgag
     1741 ctcgagcgcg gcaccaacct cctgcaggag aacgtggtgt gcctggagcg catccccgcg
     1801 ggccacaagg tcgcgacgcg cgcggtcgcg aagggcgagc ccgtgcggcg ctacagccag
     1861 atcatcggct tcgcgtcgca gccgatcgcc gcgggcgcgc acgtccacgt gcacaacctc
     1921 gaggtgcgcg atttcgcccg cgactacgca ttcggcgagg cttaccggcc gacccagttc
     1981 gcgagcgagc ccgcgatgtt caagggcatc gtgcggcccg atgggcgcgt ggccacgcgc
     2041 aactacgtcg gcatcctctc gacggtgaac tgctcggcga cggtcgcgaa gcacgtggcg
     2101 aaggcgttcg aaggcgaggc gctcgcgtcc tatcccaacg tcgacggcgt cgtcgcgctc
     2161 acgcactcgg cgggttgcgg catggatgcg agcggcgaag gcatggacgt gctgcgccgc
     2221 acgctcgccg gctatgcgcg gcatgcgaac ttcgcgggcg tgctcgtcat cggcctcggc
     2281 tgcgagggca accagatgga cgcgctcttc ttcacgcagg gcctcgcgga ggggccgctg
     2341 ctgcgcgcga tgaccatcca ggacaagggc ggcacggcga aggcggtcgc cgaaggcatc
     2401 gcgatcgtga aggagatgct tcccgtcgcg aaccaggtcg agcgctcgcc ggttcccgcg
     2461 agcgccatcg tggtgggcct gcagtgcggc ggctccgacg gctactcggg catcacggcc
     2521 aatccggcgc tgggggccgc ggtcgacctg ctggtgcgcc acggcggcgg cgcgatcctg
     2581 tcggaaacgc cggaaatcta tggcgccgag cacttgctca cgcgccgcgc ggtcacgc
//
LOCUS       DASVII010000275         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_49259, whole
            genome shotgun sequence.
ACCESSION   DASVII010000275 DASVII010000000
VERSION     DASVII010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49259"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..868
                     /locus_tag="VF309_03585"
     CDS             107..868
                     /locus_tag="VF309_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7465699.1"
                     /translation="MRFTSLGSGSSGNALVVESGRTRVMMDCGFSTADTKARLERAGL
                     SPADISAIVVTHEHDDHIGGVARFAQRYAIPVYLTRGTAQWLPPEFPAVLVHRIESHR
                     PFAVNELAVDPFPVPHDAREPVQFVFSDGDARLGVVTDLGTITQHVVEKLSGCHALVI
                     ECNHDLDMLMTGPYPDGLKRRVSGRFGHLCNADSGRLVAALDRGKLRHLIAAHLSQQN
                     NTPALAIAALARAAGCEDEWIGVATQDAGFAWRDA"
     gene            complement(947..1225)
                     /locus_tag="VF309_03590"
     CDS             complement(947..1225)
                     /locus_tag="VF309_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465700.1"
                     /translation="MKSRLILAAVVAFGIAGCTKTEVTPVPTTTTKVEVKPAEAPAAA
                     PASSTTTTTAPVDASGASSTTTTTTPPAPAAAPTTTTTTTAPADAPKK"
     gene            complement(1367..1879)
                     /locus_tag="VF309_03595"
     CDS             complement(1367..1879)
                     /locus_tag="VF309_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465701.1"
                     /translation="MSEGSDSTKPAEPVYRQIFGIAESLAGIDEVIATARQTIRVFDI
                     SLSNRGFNSPARATLLREFFVRGRAHRLHIALHDTDLLERECPRLLVLLRQFPMSIEI
                     HRTIAQARNASDPCVIADDHSVWHQLHFEQPRAVLALHSVTDALPIRQRFDEIWDLSE
                     PAVSATTLGL"
     gene            complement(1876..3027)
                     /locus_tag="VF309_03600"
     CDS             complement(1876..3027)
                     /locus_tag="VF309_03600"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7465702.1"
                     /translation="MTRKHPTSLFGGLIGGLDIEAFMLRHWQREPLLVRGAFPGFADP
                     LTPREVLALATDADASSRLVRHRGASWSLEHGPFSPSRFKQLPRRDWTVLVQDTNHFS
                     ARADELLGCFDFIPHARIDDVMVSYAVPGGSVGPHVDSYDVFLLQGHGKRRWQISGQK
                     DHSFVPGLDLKILERFIPDEEWVLEAGDMLYLPPGIAHHGVAESECLTRSIGFRAPTD
                     AELVTGFLDFLRDRLAPAGQYRDPGAAPARHPGEIPPGLIAHAASAMESIHWNGALLR
                     EFTGRFLSEPKAHVYFTPPKRPLTRAQFAREAARRGVALDPRSRLMFSGTMFFLNGEA
                     AGVPAKARAMLQSLADGRSIAGPVEAPREFWDVTHGWYLQGYAHAGETP"
     gene            3074..3556
                     /locus_tag="VF309_03605"
     CDS             3074..3556
                     /locus_tag="VF309_03605"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7465703.1"
                     /translation="MLHVGQPAPSFQLPDASMEMMSLSSYRGKKNVVLYFYPKDGTPG
                     CTIQAIGFSDRENEFNKLDTVVFGVSRDDVLSHQAFRDEHGLSVQLLADTEEEVCRLY
                     QVIQDKIVDGHPKACLLRSTFIIDKKGVVRHILQDVNPRAHVEEVLKVCRSLDFAHAN
                     "
BASE COUNT          611 a         1126 c         1267 g          586 t
ORIGIN      
        1 cgggctcctc tacgaacagc ttaagtagga agcgctgcgt gaggagcacc agcgggaacg
       61 gcgttggagc ggacggttcc cgccgctcaa cgccaagagg cggatgatgc gtttcacgtc
      121 gctgggcagc ggcagctccg ggaacgcgct cgtcgtcgag agcggccgca cgcgcgtcat
      181 gatggactgc ggcttctcca ccgcggacac caaggcgcgc ctcgagcgcg ccggcctctc
      241 gcccgcggat atctcggcga tcgtggtcac ccatgagcac gacgatcaca tcggcggggt
      301 cgcgcgcttc gcgcagcgct acgcgattcc ggtctatctc acgcgcggca ccgcgcaatg
      361 gctgccgccg gaatttcccg cggtgctcgt tcaccgcatc gaatcgcacc ggccgttcgc
      421 cgtgaacgag ctcgcggtcg atccgttccc ggtgccccac gacgcgcgcg agcccgtgca
      481 gttcgtgttc tccgatggcg acgcgcggct gggcgtggtc accgaccttg gaaccatcac
      541 gcagcacgtg gtggagaagc tctccggttg ccatgcgctc gtgatcgagt gcaaccacga
      601 cctcgacatg ctgatgaccg ggccgtaccc cgacggcctc aagcgccgcg tgtcgggacg
      661 cttcgggcac ctgtgcaacg ccgattcggg gcggctcgtc gccgcgctcg accgcggcaa
      721 gctgcgccac ctcatcgcgg cgcatctttc gcagcagaac aacacgcccg cgctcgcgat
      781 cgccgcgctc gcccgggcgg cgggctgcga agacgaatgg atcggcgtcg ccacgcagga
      841 tgcgggcttc gcctggcggg atgcctagca gaaacgaaac aagtaaaaaa gggccaggct
      901 caaattgagc ctggcccctt ttttacttgt tgctgcgtga aacgaactac tttttcgggg
      961 cgtcggccgg ggccgtcgtc gtcgtggtgg tggtcggagc ggcagccggg gccggcgggg
     1021 tcgtggtcgt ggtggtgctg ctcgcaccgc tcgcgtcgac gggtgccgtc gtggtcgtgg
     1081 tgctgctcgc gggggctgcg gcgggcgcct cggcgggctt cacttcgacc ttggtggtgg
     1141 tcgtgggcac gggagtgact tcggtcttgg tgcagccggc gatgccaaag gcgacgacgg
     1201 cggcgaggat caggcgcgat ttcatcgttc gaattccttc agcgggtggt tgttcggtaa
     1261 gaggcgtgag ctgctccggg attacgcagg cgtttgatgc actgcgatgg cagcggttcc
     1321 cgaggctaat gccagcgcaa cattatacgg aatttgctgc ggagcatcaa agccccaggg
     1381 tagtcgccga gacggccggc tccgagagat cccagatctc gtcgaaacgc tggcggatcg
     1441 gcagcgcatc ggtgaccgag tgcagcgcga gcacggcgcg cggctgctcg aaatggagct
     1501 ggtgccacac gctgtggtcg tcggcgatca cgcaggggtc cgacgcattg cgcgcctggg
     1561 cgatggtgcg gtggatctcg atcgacatcg ggaactggcg cagcaggacg agcagccgcg
     1621 ggcattcgcg ctcgaggaga tccgtgtcgt gcagcgcgat gtggagccga tgggcgcggc
     1681 cgcgcacgaa gaactcgcgc agcagggtcg cgcgcgcggg ggagttgaag ccgcggttgc
     1741 tgagggagat gtcgaagacg cggatggtct gccgggcggt ggcgatgacc tcgtcgatgc
     1801 cggccaggga ttccgcaatg ccgaagatct gccggtagac cggttcggcg ggcttcgtgg
     1861 agtcggatcc ttcgctcatg gtgtctcccc tgcgtgcgcg tagccttgca gataccagcc
     1921 gtgggtcacg tcccagaatt cgcgcggcgc ctcgacaggg cccgcgatgc tgcgcccgtc
     1981 ggcgaggctt tgcagcatcg cgcgggcctt cgcgggcacc ccggcggcct ccccgttcaa
     2041 gaagaacatc gtgccagaaa acatgaggcg cgagcgcgga tcgagcgcga cgccccggcg
     2101 cgcggcttcg cgcgcgaatt gggcgcgagt gagcggcctc ttcggcgggg tgaagtaaac
     2161 gtgcgccttc ggctccgaga ggaagcgccc ggtgaactcg cgcagcaacg caccattcca
     2221 gtgaatcgac tccatcgcgc tcgccgcgtg cgcgatgagg cccggcggaa tctcgcccgg
     2281 atgccgcgcg ggcgccgcgc ccggatcgcg gtactggccg gcgggcgcga ggcggtcgcg
     2341 caggaaatcg aggaagcccg tgacgagctc cgcgtcagtc ggagcgcgaa agccgatgga
     2401 ccgtgtgagg cattccgact cggcgacgcc gtggtgcgcg atgccgggcg gcaggtagag
     2461 catgtccccg gcctcgagca cccactcctc atcggggatg aagcgctcga ggatcttcag
     2521 gtccaggccg ggaacgaacg aatggtcctt ctgccccgaa atttgccagc gccgcttgcc
     2581 gtgcccctgc aggaggaaga cgtcgtagga gtccacgtgc gggccgacgc tgccgcccgg
     2641 gacggcgtag ctcaccatca cgtcgtcgat gcgcgcgtgc gggatgaagt cgaagcagcc
     2701 gagcagctcg tcggcgcgtg ccgagaagtg gttggtgtcc tgcaccaaca cggtccagtc
     2761 ccggcgtgga agttgcttga accgcgaggg agaaaacggc ccgtgctcga gcgaccacga
     2821 tgcaccgcga tgacgcacga ggcgcgacga tgcatcggcg tcggtcgcga gcgcgaggac
     2881 ctcgcgtgga gtgagcggat cggcgaagcc gggaaacgcg ccgcgcacga ggagcggctc
     2941 gcgctgccaa tggcgcagca tgaacgcctc gatatcgagg ccgccgatga ggccgccgaa
     3001 gagggatgtg gggtgcttgc gtgtcatcaa aatggattag aatcgattcg acagtcgagc
     3061 gattgcgggt gccatgctac acgtaggcca accagcacca tccttccagc tccccgacgc
     3121 cagcatggag atgatgtctc tctccagcta ccggggaaag aagaacgtcg tcctttattt
     3181 ctacccgaag gacggcacgc ccggctgcac catccaggcg atcggcttca gcgatcgcga
     3241 gaacgagttc aacaagctgg atacggtggt gttcggtgta tcgcgcgacg acgtgctttc
     3301 ccaccaggcg tttcgcgacg agcacggcct gtcggtgcag ctcctcgccg acaccgagga
     3361 ggaagtctgc cgcctctacc aggtgatcca ggacaagatc gtcgacggcc accccaaggc
     3421 gtgcctgctg cggtccacct tcatcatcga caagaagggc gtggtgcgcc atatcctcca
     3481 ggacgtgaat ccgcgcgccc acgtggagga agtcctgaaa gtgtgcaggt ccctagattt
     3541 cgcgcatgcc aattgaccgc ggcacggtgg tcacgctcga atacgagctg
//
LOCUS       DASVII010000276         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_49266, whole
            genome shotgun sequence.
ACCESSION   DASVII010000276 DASVII010000000
VERSION     DASVII010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49266"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF309_03610"
     CDS             <1..225
                     /locus_tag="VF309_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7465704.1"
                     /translation="TNVGISLPGIFVGSFLLEVFFSIPGLGREIITAVNRSDFTVIKA
                     VTVYLAVITMFINLLVDVMYKVVDPRVSFK"
     gene            235..1305
                     /locus_tag="VF309_03615"
     CDS             235..1305
                     /locus_tag="VF309_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465705.1"
                     /translation="MSAVLQNPPLPPMQESPGLWVLAWRRMRQDTVGMVSLVIVAIFI
                     AMMAGSYAGLIAKDWNKEKGVSYANPTFLAGAENLEAKSMAAKDATAKAPPVDLSDID
                     PLAPRYKEWEERAARIAVTETKRAEVLPFGGDKWGRDVLQKAIKGSQISIFVGLSAAL
                     LATFIGTMLGAAAGYWGGWANDVLEWIYNVFTSIPYILLILAFAAVATATGKFGKGIL
                     PIVLILSLTGWTGIYRLMRAEYIKHSNREYVKAAQAIGASNWSRMFIHILPNVSHVAL
                     VQLSQHVVGFIKAEVILSFLGFGVPVDMVSWGTMLAEAQNELVIGKWWQLFAAGASMA
                     VLVTAFSLLTDSLRDALDPKLK"
     gene            1302..>1645
                     /locus_tag="VF309_03620"
     CDS             1302..>1645
                     /locus_tag="VF309_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465706.1"
                     /translation="MTNILQIQDLDIAFRIDRKSEPFRAVKAISFDVPTNTTVALVGE
                     SGSGKSVTAMSILGLLPQNAIVAPSGRITYFGSRLGSSGGRDLLKSSRNELQNIRGKD
                     ISVIFQEPMTSL"
BASE COUNT          277 a          551 c          511 g          306 t
ORIGIN      
        1 accaacgtcg gcatctcgct cccgggcatc ttcgtgggct cgttcctgct ggaagtgttc
       61 ttctcgatcc cgggcctcgg ccgcgagatc atcaccgccg tgaaccgcag cgactttacc
      121 gtcatcaagg cggtgacggt ctacctcgcg gtcatcacca tgttcatcaa cctgctggtc
      181 gacgtgatgt acaaggtcgt cgacccgcgc gtgtcgttca aatagggagc attcatgagc
      241 gcagtcctcc agaatcctcc cttgccgccg atgcaggaat cgccgggcct ctgggtcctc
      301 gcgtggcggc gcatgcgcca ggacacggtc ggcatggtct cgctcgtgat cgtggcaatc
      361 ttcatcgcga tgatggccgg gtcgtacgcg gggctcatcg cgaaggactg gaacaaggag
      421 aagggcgtgt cgtacgccaa tccgacgttc ctcgccggcg ccgagaacct cgaggcgaag
      481 tcgatggccg cgaaggacgc gacggcgaag gcgccccccg tggacctctc cgacatcgat
      541 ccgctcgcgc cgcgctacaa ggagtgggag gagcgcgccg cgaggatcgc ggtgaccgag
      601 accaagcgcg ccgaagtgct gcccttcggc ggcgacaagt ggggccgcga cgtcctgcag
      661 aaggcgatca agggctccca gatctccatc ttcgtggggc tctccgcggc gctgctcgcg
      721 accttcatcg gcaccatgct gggcgcggcc gcgggttact ggggcggctg ggcgaacgac
      781 gtcctcgagt ggatctacaa cgtcttcacg tccattccct acatcctgct gatactcgcg
      841 ttcgccgcgg tcgccacggc cacgggcaag ttcggcaagg gcatcctgcc gatcgtcctc
      901 atcctctccc tcaccggctg gaccggcatc tatcgcctga tgcgcgccga gtacatcaag
      961 cactcgaacc gcgagtacgt gaaggcggcg caggcgatcg gcgcctcgaa ttggtcgcgc
     1021 atgttcatcc atatccttcc caacgtgagc cacgtggcgc tggtgcagtt gtcgcagcac
     1081 gtggtcggct tcatcaaggc cgaggtgatc ctctccttcc tgggattcgg cgttcccgtc
     1141 gacatggtgt cgtggggcac gatgctcgcc gaggcgcaga acgagctcgt catcggcaag
     1201 tggtggcagc tcttcgccgc cggggcctcc atggcggtcc tcgtcaccgc gttctccctc
     1261 ctcaccgatt cgctgcgcga cgcattggac ccgaagctga aatgaccaac atcctccaga
     1321 tccaggacct cgatatcgcc ttccgcatcg accggaagtc ggagccattc cgcgcggtga
     1381 aggcaatctc cttcgacgtc ccgaccaaca ccaccgtggc gctggtcggc gagtccggga
     1441 gcggcaagag cgtcaccgcg atgtcgatcc tggggctcct tccgcagaat gcgatcgtcg
     1501 cgccctcggg ccgcatcacc tatttcggct cgagactcgg ctcctcgggc gggcgcgacc
     1561 tgctgaagtc ctcgcgcaac gagctgcaga atatccgcgg caaggacatc tcggtgattt
     1621 tccaggagcc gatgacgtcg ctgaa
//
LOCUS       DASVII010000277         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_300900,
            whole genome shotgun sequence.
ACCESSION   DASVII010000277 DASVII010000000
VERSION     DASVII010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300900"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VF309_03625"
     CDS             complement(<1..1283)
                     /locus_tag="VF309_03625"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7465707.1"
                     /translation="MKKNPQSGLRKGLTSYGDSEFSLFLRKAFIKGAGYTDDALERPI
                     VGIANTASAYNPCHGNSAQLIEAVKRGVMLAGGLPMDFPTISIHESFASPTSMYLRNL
                     MSIDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAGSAGIPAVQLVTGSMLTGSHEGVR
                     VGACTDCRRYWGHYRAEEIDSDEISRVNNQLVASVGTCSVMGTASTMACVAEALGIAL
                     PGSASPPAVTADRIRIAERSGATAVELAKGKLTPDVILTAKAFENALRVLLAIGGSTN
                     GIIHLTAIAGRLGVAIDLDRVDAIGRETPVLVDLKPSGQHYMEDFHRAGGVPTLLREL
                     KPLLHLDAMTVTGRTLGEEMGRGAQAFDQDVVRTRANPIFPQGGIAVLRGNLAPGAAL
                     IKQSAANPALMEHEGRAVVFENLEDLAQRIDDDAL"
     gene            1324..>1635
                     /locus_tag="VF309_03630"
     CDS             1324..>1635
                     /locus_tag="VF309_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7465708.1"
                     /translation="MKIDSNARDVYVIAPTPFHPDGRIDDSSVDRMTDFFIRAGVDGM
                     TVLGQLGEAPKMEHNESVGIVKRVIQRAGMPIIVGVSAPGFAAMRALTHDAMAAGAAG
                     VM"
BASE COUNT          244 a          537 c          582 g          272 t
ORIGIN      
        1 tcgagcgcgt cgtcgtcgat gcgctgtgcg aggtcctcga gattctcgaa cacgacagca
       61 cggccttcat gctccatgag cgcggggttc gcggcggatt gcttgatgag ggccgcaccc
      121 ggcgctaggt tgccgcgcag caccgcgatg ccgccctgcg ggaagatggg attggcgcgg
      181 gtgcgcacca catcctggtc gaaagcctgc gcgccgcgcc ccatctcctc gcccagagtg
      241 cgcccggtga cggtcatggc gtccaggtgc aggagcggct tcagctcccg cagcagtgtc
      301 ggcacgccgc ccgcgcggtg gaagtcctcc atgtaatgct ggcccgaggg cttcaggtcc
      361 acgaggaccg gcgtctcgcg cccgatggcg tccacgcgat cgaggtcgat cgcgacgccg
      421 agtcgtccgg cgatcgccgt gagatggatg atcccgttgg tggagccgcc gatcgcgagg
      481 agcacgcgca gcgcgttctc gaaggccttc gcggtgagga tcacgtcggg ggtgagcttt
      541 cccttcgcga gctcgaccgc cgtcgccccg gatctttccg cgatgcgaat gcggtcggcg
      601 gtgaccgcgg ggggcgacgc gctccccggc aatgcgatcc cgagcgcctc ggccacgcac
      661 gccatggtgc tcgcggtgcc catcaccgag caggtgccca cgctcgcgac gagctggttg
      721 ttcacccgcg agatctcgtc ggagtcgatc tcctcggcgc ggtaatgccc ccagtagcgg
      781 cggcaatcgg tgcaggcgcc gacgcgcacg ccttcgtggg agccggtgag catcgagccg
      841 gtgacgagct gcaccgcggg gatgccggcc gagcccgcgc ccatcagctg cgcgggcacc
      901 gtcttgtcgc agccgccgat cagcaccacc gcgtccatgg gctgggcgcg gatcatctcc
      961 tcggtgtcga tggacatgag gttgcgcagg tacatcgacg tgggcgacgc gaagctctcg
     1021 tggatcgaga tcgtcgggaa atccatcggc aggccgcccg cgagcatcac gccgcgcttc
     1081 accgcctcga tgagctgcgc ggagttgccg tggcacgggt tgtaggcgct cgccgtgttg
     1141 gcgatgccga cgatggggcg ctcgagcgca tcgtcggtgt agcccgcgcc cttgatgaag
     1201 gccttgcgca ggaagagcga gaactcgctg tcgccgtagc tcgtgaggcc cttgcgcagc
     1261 ccggattgcg gattcttctt catggcgtga tgttagcggg aatcgggatg ctacgattcg
     1321 cgcatgaaaa tcgacagcaa cgccagggac gtctacgtga tcgcgccgac gccgttccat
     1381 ccggacggtc gcatcgacga ttcgtcggtc gaccgcatga ccgatttctt catccgtgcc
     1441 ggcgtggacg ggatgacggt gctcggccag ctgggcgaag cgccgaagat ggagcacaac
     1501 gaatccgtgg ggatcgtgaa gcgcgtgatc cagcgcgcgg ggatgcccat catcgtcggc
     1561 gtctcggcgc ccggcttcgc cgcgatgcgc gcgctcacgc acgacgccat ggccgcgggc
     1621 gccgcggggg tgatg
//
LOCUS       DASVII010000278         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_552883,
            whole genome shotgun sequence.
ACCESSION   DASVII010000278 DASVII010000000
VERSION     DASVII010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552883"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1547)
                     /locus_tag="VF309_03635"
     CDS             complement(39..1547)
                     /locus_tag="VF309_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7465709.1"
                     /translation="MKLFRKYADCCAGGKSEYRDPNARGLLSILAMIAAALASAALLI
                     LGGCASSAGIRSVAQTIAPKTAGLDSRAKTPELAADWWRSFDEPALNDLVERALAGNP
                     TLKVAEARMTRALAAVAGAQAAAGPQVNGELDVTRERLSATSIYPPPLGGSIVTLANG
                     QISGSWELDFFGRNRAAIDAAIGTQRAAQADVQAARILLASSIARAYGQLGRLIGQRE
                     IAERTLKQREDMLSLTRQRVQSGLDTNVELRQSEGALPDTRQQIEQLDEQIALTRHAL
                     AAFTAQPPNALDGLTVTLRSVKPIPVPAVVPADLLGRRADITAARWRIEAATSDMKSA
                     KAQFYPNINLTAFIGLNSIGLSRFLKSDSEQYGVGPALRLPIFDSGRLRANLSGKAAD
                     LDVAIESYNVAVIDAAHDTADQLASLRSVAFQQAQQASAQAAAESAYDLATQRYKAGI
                     TNYLTVLNAESTVLSQRRQFVDLKARELDVQIALIRALGGGYVPDAQRVAQH"
     gene            complement(1552..2037)
                     /locus_tag="VF309_03640"
     CDS             complement(1552..2037)
                     /locus_tag="VF309_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7465710.1"
                     /translation="MPKISIPADFYRPSAYCAEESVGYLMKRVQMSIVYQVDKRLAEH
                     GLTSAQWGPLMRMQIAGGGSTVAELARWMQVDAGAMTRLLDRLEKKGLCKRVRSTEDR
                     RVVRVELTGKGKTAMAEVPAVLAEVMNAHLAGFSKTEWNALKTYLQRMVETGDALREK
                     G"
BASE COUNT          294 a          695 c          794 g          391 t
ORIGIN      
        1 aattgggtgc gggggacgcc atggtgtatc tccgggactc agtgttgcgc gacacgttgc
       61 gcgtcgggga cgtagccgcc gccgagggcg cggatcaatg cgatctgcac gtcgagctcg
      121 cgtgccttca ggtcgacgaa ctggcggcgc tggctgagca ccgtggactc ggcattcagg
      181 accgtgaggt agttggtgat gcccgccttg tagcgctgcg tcgcgaggtc gtaggccgat
      241 tccgccgcgg cttgcgcgct cgcctgctgc gcctgctgga aggcgacgga gcgcagcgat
      301 gcgagctggt cggccgtgtc gtgcgcggca tcgatgaccg cgacgttgta gctctcgatc
      361 gcgacgtcca ggtccgcggc cttgccgctg aggttcgcgc gcaggcgccc cgagtcgaag
      421 atcggcaggc gaagcgcggg tcccacgccg tactgctcgc tgtccgactt gaggaaccgg
      481 ctcaagccga tgctgttgag gccgatgaag gcggtgaggt tgatgttcgg atagaactgc
      541 gccttcgcac tcttcatgtc gctggtcgcg gcctcgatgc gccagcgcgc ggcggtgatg
      601 tccgcgcggc ggcccaggag atcggcgggc acgacggcgg gaacgggaat cggtttcacg
      661 cttcgcagcg tcacggtcag gccgtcgagc gcgttgggtg gctgcgcggt gaacgcggcg
      721 agggcatggc gggtgagggc gatctgctcg tcgagctgct cgatctgctg gcgagtgtcg
      781 ggaagcgcgc cctcgctctg gcgcagctcc acgttcgtgt cgaggccgct ttgcacgcgc
      841 tggcgcgtga gggagagcat gtcctcgcgc tgcttcagcg tgcgctccgc gatttcccgc
      901 tgcccgatga ggcggccgag ctgcccgtag gcgcgcgcga tgctgctcgc aaggaggatg
      961 cgcgcggcct ggacgtcggc ttgtgccgcg cgctgcgtgc cgatcgcggc atcgatcgcc
     1021 gcgcggttgc ggccgaagaa atcgagctcc cacgagccgc tgatctgccc gttcgcgagc
     1081 gtcacgatcg agccgccgag cggcggcggg tagatgctgg tcgcgctcag tcgctcgcgc
     1141 gtcacgtcga gctcgccgtt gacctgcggg cccgcggcgg cctgcgcgcc cgcaacggcg
     1201 gcgagggcgc gagtcatgcg tgcctccgcg accttgaggg tcgggttgcc ggcgagcgct
     1261 cgctcgacga ggtcgttcaa cgcgggctcg tcgaagctgc gccaccagtc ggccgcgagc
     1321 tcgggcgtct tcgcccggga atcgaggccc gcggttttgg gagcgatcgt ttgcgcgacg
     1381 gacctgattc cggcgctgct cgcgcaaccg cccaggatga gaagcgccgc tgaggcgagg
     1441 gcggcggcga tcatcgccag gatcgaaagg agcccgcgcg cattcggatc gcggtattcg
     1501 ctcttgccgc cggcgcagca gtccgcgtac ttccggaaga gtttcatggc atcagccttt
     1561 ttcgcgcagc gcgtcgccgg tctcgaccat gcgctggagg tatgtcttga gggcattcca
     1621 ttcggtcttg gaaaagccgg cgagatgcgc gttcatcact tccgcgagca ccgcaggcac
     1681 ctcggccatc gccgtcttcc ccttgccggt gagctcgacg cgcaccacac gccggtcctc
     1741 ggtcgagcga actcgcttgc acaggccctt cttctcgagg cggtcgagga gccgcgtcat
     1801 ggcgccggca tcgacctgca tccaccgcgc gagctccgcc accgtcgagc cgcctccggc
     1861 gatctgcatt cgcatgagcg gcccccactg cgcgctcgtg aggccgtgtt cggcgagccg
     1921 cttgtccacc tggtacacga tggacatctg cacgcgcttc atgaggtagc cgacgctttc
     1981 ctcggcgcaa taggcgctgg ggcggtagaa gtcggcgggg atggagatct tgggcatggc
     2041 gtcctcgggg gaggtttgcc atgactatat ttgcctaggc aacacttgtc aaggctgttg
     2101 ttgtgcggca gcctggtccc gcggcggccg gcggggccgt tgcggggccg tggaacatcg
     2161 cgtgcgctaa gata
//
LOCUS       DASVII010000279         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_660637,
            whole genome shotgun sequence.
ACCESSION   DASVII010000279 DASVII010000000
VERSION     DASVII010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660637"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VF309_03645"
     CDS             complement(<1..641)
                     /locus_tag="VF309_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7465711.1"
                     /translation="MRDRPSPHHKLEWPGGVVPEWARPQQNSNKRRGGKGKGPGGGNE
                     RQGGSSPSKRERLQKVMAQSGHGSRRNIEILIAQGHITINGQVAKLGDSVGPGDKVKL
                     DGKLLGLRFGEALPRVLLYHKTAGEIVTRDDPEKRPTVFDKLPKVHGGRWVSVGRLDF
                     NTEGLLIFTTSGELANRLTHPRFEVEREYAVRVMGELSEEQKTKLLTGVDIEG"
     gene            complement(631..1398)
                     /gene="scpB"
                     /locus_tag="VF309_03650"
     CDS             complement(631..1398)
                     /gene="scpB"
                     /locus_tag="VF309_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7465712.1"
                     /translation="MNEQTNPEQLPDAEERDEAPLPPDTDLAAQEAPEIDIGHVKRVL
                     EAALLSTAEPLTVQQLKRLFAGEVDADNIRKVLDQLKDEWAERSIELTAVASGWRFRV
                     KPDYQKFLDRISSEKPPRYSRAVLETLAIIAYRQPVTRGDIEEVRGVAVSPPTINALM
                     ERGWVEIVGTRETPGRPAIFATTRKFLDDLNLRSLEELPPLEDLQSALEPASASLLAL
                     EAASSPLQYVPQEEESPEERSTDEPQTPESSEQAHAG"
     gene            complement(1423..2277)
                     /locus_tag="VF309_03655"
     CDS             complement(1423..2277)
                     /locus_tag="VF309_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX7465713.1"
                     /translation="MSTQAAHDSVPGLAQHGLDLALPIAKIRGEPMTEMPADLYIPPE
                     ALSVFLEAFEGPLDLLLYLIRRHSLDILDIPMADLTRQYMSYVEMMRTTQLELAADYL
                     LMAAVLIEIKSRMLLPRPKREDENEPDDPRAELVRRLLEYERMKKAAYAISDIPVAGR
                     DFSLVEVYVEQALAGALPDVQVADLSEAWRSILARARMTKHHRITREQLSVRAHMSRI
                     LKALAPGAFVEFSELFEPERGVALLVVSFLACLELARESMIEITQQTPFDPIYVKIKA
                     ERATLTIA"
BASE COUNT          330 a          797 c          873 g          432 t
ORIGIN      
        1 tcgccctcga tgtcgacgcc cgtcagcagc ttcgtcttct gctcctcgct cagctcgccc
       61 atcacgcgca ccgcgtattc gcgctcgacc tcgaagcgcg ggtgcgtgag gcggttcgcg
      121 agctcgccgc tcgtcgtgaa gatgagaagc ccctcggtgt tgaagtcgag gcggccgacg
      181 gacacccagc gcccgccgtg caccttcggc agcttgtcga acaccgtggg gcgcttctcc
      241 gggtcgtcgc gcgtgacgat ctcgcccgcg gtcttgtgat agagcagcac gcgcggcagc
      301 gcctcgccga agcgcagccc gaggagcttg ccgtcgagct tcaccttgtc gccggggccg
      361 accgagtcgc cgagcttcgc cacctggccg ttgatggtga tgtggccctg ggcgatgagg
      421 atctcgatat tgcgccgcga gccgtggccc gactgcgcca tgaccttctg cagccgctcg
      481 cgcttcgagg ggctcgagcc tccctggcgc tcattgccgc cgccgggccc cttgcccttg
      541 ccgccgcggc gcttgttgga attctgttgc ggacgggccc attccggcac gacgccgccg
      601 ggccattcga gcttgtggtg gggggagggt ctatcccgca tgggcttgct ccgaggactc
      661 tggggtttgc ggctcgtccg ttgatctttc ttcgggcgat tcttcttcct gcggtacgta
      721 ttgcagcggc gacgaggccg cctcgagcgc gaggaggctg gccgaggccg gctcgagcgc
      781 cgactgcagg tcctccaggg gcggaagctc ctcgagcgat cgcaggttca ggtcgtcgag
      841 gaatttccgg gtggtggcga agatggcggg ccggccgggc gtttcgcgcg tgccgacgat
      901 ctcgacccag ccgcgctcca tgagcgcgtt gatggtgggc ggggagacgg cgacaccgcg
      961 cacctcctcg atgtcgccgc gcgtgaccgg ctggcggtac gcgatgatgg cgagcgtctc
     1021 cagcaccgcg cgcgaatagc gcggcggctt ctcgctggag atgcggtcga ggaacttctg
     1081 gtagtccggc ttcacgcgga agcgccagcc cgaggcgacc gcggtgagct cgatggatcg
     1141 ctcggcccac tcgtccttca gctggtcgag caccttgcgg atgttgtcgg cgtccacctc
     1201 gcccgcgaag agccgcttca gctgctgcac cgtgagcggc tccgccgtgg aaaggagcgc
     1261 ggcctcgagc acgcgcttca cgtgcccgat gtcgatctcg ggcgcctctt gcgccgcgag
     1321 atcggtgtcg ggaggaagcg gcgcctcgtc gcgctcttcg gcgtcgggga gctgctcggg
     1381 attcgtctgt tcgttcatgg gggtctcggg caattacctg gcctaggcga tggtgagcgt
     1441 cgcgcgctcg gccttgatct tcacgtagat ggggtcgaag ggcgtctgct gcgtgatctc
     1501 gatcatcgac tcgcgcgcga gctcgaggca cgcgaggaag ctcacgacga gcagcgcgac
     1561 gccgcgctcg ggctcgaaga gctcggagaa ctcgacgaac gcgccgggtg cgagggcttt
     1621 caggatgcgg ctcatgtgcg cgcgcaccga cagctgctcg cgcgtgatgc gatggtgctt
     1681 cgtcatccgg gcgcgcgcga gtatcgagcg ccacgcttcc gacagatcgg ccacctggac
     1741 gtcggggagc gccccggcga gcgcctgctc gacgtagacc tccaccagcg agaagtcgcg
     1801 ccccgccacg gggatgtcgg agatcgcgta cgcggccttc ttcatgcgct cgtactcgag
     1861 caggcgccgc acgagctcgg cgcgcggatc gtcgggctcg ttctcgtcct cgcgcttcgg
     1921 ccgcggcagc agcatccgcg acttgatctc gatgaggacg gcggccatca gcagatagtc
     1981 ggcggcgagc tcgagctgcg tcgtgcgcat catctcgacg tacgacatgt actggcgcgt
     2041 gaggtccgcc atcgggatgt cgaggatgtc gagcgagtgg cggcggatga ggtagagcag
     2101 caggtcgagc gggccctcga aggcctcgag gaacaccgac agcgcctcgg gcgggatgta
     2161 gagatccgcc ggcatctcgg tcatcggctc gccgcggatc ttcgcgatcg ggagcgcgag
     2221 atcgagcccg tgctgcgcga gcccggggac ggaatcgtgg gcggcctgcg tgctcatggc
     2281 ttcttgaaca tcgcgtcggc ggcgagcttg ctcatcgctt cgccgttgtg cgcggcctcg
     2341 gggacttcga cgagctccca cttgaggggc acgccgagat ccgcggcggc ggccgtggcc
     2401 gccttgaaga agctctccgc gcggtcgaca cg
//
LOCUS       DASVII010000280         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_337196,
            whole genome shotgun sequence.
ACCESSION   DASVII010000280 DASVII010000000
VERSION     DASVII010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337196"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VF309_03660"
     CDS             <1..495
                     /locus_tag="VF309_03660"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7465714.1"
                     /translation="RERVAARLAERVAALPEYAAARSVLATMAIGSEWSTRPFLDRAR
                     ADGKTIVLPRVTTPPRHLEIHAVENLDRDLVTGVWDIPEPDPERCRRLEFAEVDFALV
                     PALAVDSAGYRLGYGAGYFDRLLAGRGARPFCLTALPPAFLVASLPHEAHDVPVDRVL
                     SLAP"
     gene            492..1373
                     /gene="folD"
                     /locus_tag="VF309_03665"
     CDS             492..1373
                     /gene="folD"
                     /locus_tag="VF309_03665"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX7465715.1"
                     /translation="MTARIIDGKAIAAEYRAEYAIRVERLKDHGIVPGLAVVIVGDDP
                     ASKIYVRNKALASGAIGMHSEVHAFAAEVTQAQLIAFVHRLNANDAIHGILVQLPLPK
                     HIDSRAVIEAIAPDKDVDGFHYFNVGSLVAGEPGFYPCTPWGVMKMLEHEDIAIEGRH
                     AVVVGRSSIVGKPMALLLLNAGATVTICHSKTRDLGAMTRQADILVAAVGRAGLVTGD
                     MVKPGAVVIDVGINRLPDGKLTGDVDFPGASQVAWRITPVPGGVGPMTIAMLLGNTVK
                     SAERVAGLAQLAETRIA"
     gene            1387..>1689
                     /locus_tag="VF309_03670"
     CDS             1387..>1689
                     /locus_tag="VF309_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7465716.1"
                     /translation="MNPYAKGLERNAANYTALTPVSFLAKAASVYPGRIAVVHGAVRR
                     TWKEVYERSRRLASALAKHGVKRGETVAAMLPNVPAMVELHFGPAMFGAVLNTLNTR"
BASE COUNT          267 a          580 c          581 g          261 t
ORIGIN      
        1 cgtgagcgcg tcgccgcgcg cctcgccgag cgcgtcgcgg cgcttcccga atatgccgcc
       61 gcgcgctcgg tcctcgcgac catggcgatc ggcagcgaat ggagcacgcg gccgttcctc
      121 gatcgcgcgc gcgccgacgg caagacgatc gtgttgccac gggtgacgac gccgccgcgc
      181 cacctggaga tccatgcggt cgagaacctc gatcgcgacc tcgtgaccgg cgtatgggac
      241 attcccgagc ccgatcccga gcggtgccgc aggctcgaat tcgccgaggt ggatttcgcg
      301 ctcgtcccgg cgctcgccgt cgattccgcg ggctaccggc tcggatacgg cgcgggctat
      361 ttcgatcggc tgctcgcggg gcgcggcgcg cggcccttct gcctgacggc gcttcccccg
      421 gcgttcctcg tcgcaagcct cccgcacgaa gcccacgacg tgccggtcga tcgcgtgttg
      481 agcctcgcgc catgacggcg cgcatcatcg atggaaaggc gatcgccgcg gaatatcgcg
      541 ccgagtacgc gattcgcgtc gagcgcctga aggaccacgg catcgtcccg gggctcgcgg
      601 tcgtgatcgt cggcgacgat cccgcgagca agatctacgt gcgtaacaag gcgctcgcct
      661 cgggcgccat cggcatgcat tcggaagtcc acgcattcgc cgcggaggtc acgcaagcgc
      721 agctcatcgc cttcgtgcat cggctgaacg ccaatgacgc gatccacggc atcctcgtgc
      781 agctgccgct gccgaagcac atcgacagcc gcgccgtgat cgaggcgatc gcgcccgaca
      841 aggacgtgga cggcttccac tacttcaacg tcggctcgct cgtcgccggc gagccgggct
      901 tctacccgtg cacgccgtgg ggcgtcatga agatgctcga gcacgaggac atcgcgatcg
      961 aggggcggca tgccgtcgtg gtggggcgct cgagcatcgt ggggaagccc atggcgcttc
     1021 tccttctcaa cgcgggagcc acggtgacca tctgccattc gaagacccgc gacctgggag
     1081 ccatgactcg ccaggccgac atcctcgtcg ctgccgtggg aagggcgggg ctcgtgacgg
     1141 gcgacatggt gaagcccggc gcggtcgtga tcgacgtggg aatcaatcgc cttcccgacg
     1201 gcaagctcac gggcgacgtc gatttccccg gcgcatcgca ggtcgcctgg cgcatcacgc
     1261 cggtgcccgg cggcgtgggc ccgatgacga tcgccatgct gctgggcaat acggtgaagt
     1321 cggcggaacg cgtcgcggga ctcgcgcaac tcgccgagac gcgcatcgca tgaaggggag
     1381 ggtcgaatga atccatacgc gaaggggctc gagcgcaacg ccgccaacta cacggcactc
     1441 accccggtgt cgttcctcgc caaggccgcc agcgtctatc ccgggcgcat cgcggtggtt
     1501 cacggcgcgg tgcgccgcac gtggaaggaa gtctacgagc gctcgcggcg cctcgcgtcg
     1561 gcattggcga agcacggcgt aaagcgtggg gagaccgtcg cggcgatgct gcccaacgtg
     1621 cccgccatgg tcgagcttca cttcgggccg gcgatgttcg gagcggtgct gaatacgctg
     1681 aacacgcgc
//
LOCUS       DASVII010000281         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_409004,
            whole genome shotgun sequence.
ACCESSION   DASVII010000281 DASVII010000000
VERSION     DASVII010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409004"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1853
                     /locus_tag="VF309_03675"
     CDS             147..1853
                     /locus_tag="VF309_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX7465717.1"
                     /translation="MFRTQYLDYAELTQQLDRWARSHPGLVRISSLGKTAEGRDIPLV
                     TIGRDPGEARPAVWVDGNMHATEVCGSSVALAIAEDVINIHLDSRLHGNDTLPPHMAD
                     AIKEALFYVVPRISPDGAEAVLKSGRYVRSSPIDARLHKGHAYWEAHDFDGDGTIGFM
                     RQACDDGELVELTDHPGVMVPRMPEDPPPYFKLYPEGRIVNFDGRRIPDPYFLSDNQY
                     DFNRNFPYSWAPDHEQEGAGHYPGSAPETRAIMDFATAHPEIMAWLNLHTFGGVLIRP
                     LGDRPDDKMDQQDLAIYRQVEAWMTEFTGYPTVSGFHEFLYQPEKPLKGDLTDYAYHQ
                     RGALAYVIELWDLFTQIGIERKKPFVDVYSRFDRKDFIALAKWDREVNKGRVFGKWRK
                     VKHPQLGDVEVGGLDTRVGISNPPYEKIAEVCASQSRAFLRVAALIPRVAIEIVKQER
                     LGADLTRIELRIVNRGYLGTYGLNSAKKLPLSEPMRLTAEGSGVKVAAPAEAVIEIGH
                     LDGWGQGLYNGANVFFPWTRGNVHEKFVTLVVEGKGKLKVKVGSVRVGYLEKEIAVPA
                     AS"
BASE COUNT          349 a          640 c          620 g          304 t
ORIGIN      
        1 ggatcgcaag gccttttaca aggggcgttc gcggagcggg cggtatagta ggcaccgaat
       61 ctcgtatgcc gcttccgacg ccgagcttgc cgaggcgatg acgaggcttt cgcgcgcttg
      121 cgtccaactc cgatagacga gaatccatgt tccgaaccca ataccttgac tacgcagagc
      181 tcacccaaca actcgaccgc tgggccagga gccatcccgg cctcgtgcgc atctcgtccc
      241 tcggcaagac cgccgagggc cgcgacattc cgctcgtcac catcggccgc gaccccggcg
      301 aagcgcgccc cgcggtgtgg gtcgacggca acatgcacgc gaccgaagtg tgcggctcga
      361 gcgtggcgct cgccatcgct gaagacgtga tcaatatcca cttggattcc cgtcttcacg
      421 ggaatgacac cctgccaccg cacatggccg acgccatcaa ggaggcgctc ttctacgtgg
      481 tgccgcgcat ctcgcccgac ggcgccgagg cggtgctgaa gagcgggcgc tacgtgcgct
      541 cgagccccat cgacgcgcgc ctgcacaagg gccacgcgta ctgggaggcg cacgacttcg
      601 acggcgacgg caccatcggc ttcatgcgcc aggcgtgcga cgacggcgag ctcgtggagc
      661 tcacggacca tccgggcgtg atggtgccgc gcatgcccga ggatccgccg ccctacttca
      721 agctctatcc cgaggggcgc atcgtcaact tcgacggccg gcgcatcccg gacccgtatt
      781 tcctctcgga caaccagtac gacttcaacc gcaacttccc ctacagctgg gcgccggatc
      841 acgagcagga aggcgccggc cactatccgg gcagcgcgcc ggagacgcgc gccatcatgg
      901 acttcgcgac cgcccatccg gaaatcatgg cgtggctgaa tctccacacg ttcggcggcg
      961 tgctcattcg tcccctgggc gacaggcccg acgacaagat ggaccagcag gacctcgcga
     1021 tctatcgcca ggtcgaggcg tggatgaccg agttcacggg atatcccacg gtgagcggct
     1081 tccacgaatt cctctaccag cccgagaagc cgttgaaggg ggatctcacc gactacgcgt
     1141 accaccagcg cggggcgctc gcgtacgtga tcgagctctg ggacctgttc acgcagatcg
     1201 ggatcgagcg caagaagccc ttcgtcgacg tgtattcgcg cttcgaccgc aaggatttca
     1261 tcgcgctcgc gaagtgggac cgcgaggtca acaagggccg cgtcttcggg aagtggcgca
     1321 aggtgaagca cccgcaattg ggagacgtcg aagtgggcgg cctcgacacg cgcgtgggca
     1381 tctccaatcc tccctacgag aagatcgccg aggtgtgcgc ttcgcaaagc cgcgccttcc
     1441 tgcgcgtggc ggcgctcatt ccgcgcgtcg cgatcgagat cgtgaagcag gagaggctcg
     1501 gcgccgatct cacgcgcatc gagctgcgga tcgtcaaccg cggctatctc ggcacctacg
     1561 ggctcaactc cgcgaagaag ctgccgcttt cggagccgat gcgcctcacg gcggaaggat
     1621 ccggcgtgaa ggtggccgcg ccggccgagg cggtaatcga gatcggccac ctcgacggct
     1681 ggggccaggg gctctacaac ggcgccaacg tgttcttccc gtggacgcgc ggcaacgtgc
     1741 acgagaaatt cgtgaccctc gtcgtcgaag gcaagggcaa gctcaaggtg aaggtcggca
     1801 gcgtgcgcgt gggctacctc gagaaggaga tcgcggttcc ggccgcgtca tgatcgcttc
     1861 gcggggcgcg gcattcatgc tcgcgctcgt agctgcgctg gcacgcgccg cgg
//
LOCUS       DASVII010000282         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_301017,
            whole genome shotgun sequence.
ACCESSION   DASVII010000282 DASVII010000000
VERSION     DASVII010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301017"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF309_03680"
     CDS             complement(<1..424)
                     /locus_tag="VF309_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX7465718.1"
                     /translation="MGGPIEERNVGSVAVVFSRGLAGLDAPLVRVEAHVGGGLPQFHI
                     VGLPETEVKEARDRVRAALSTARFDFPAGRVTVNLAPADLPKESGRFDLPIAIGILAA
                     TGQLPSRGLGELEFAGELALSGELRPIRGALAMTFRAHR"
     gene            complement(453..698)
                     /locus_tag="VF309_03685"
     CDS             complement(453..698)
                     /locus_tag="VF309_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX7465719.1"
                     /translation="MNENLLSELAARLAAMAAANPAKDLEKNMRGLLASALTRLDLVS
                     REEYDVQTQVLSRAREKLVALETRVAELEARAAAKQP"
     gene            913..1719
                     /locus_tag="VF309_03690"
     CDS             913..1719
                     /locus_tag="VF309_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX7465720.1"
                     /translation="MKNLPKVLLLAGCAGLAMAVGIARAEDKPADAAAAPAAPTPEHT
                     FTGKAALYSEYEYRGISQTSEKPALQLNLDYAHSSGLYLGTFLSNIKWLKDTASANGF
                     STSAGIEWDIYGGYKFEVVKDVTLDIGYLRYEYPSSSEFSPKPNTDEVYIGASYGPVS
                     AKYSYSFNDTFGVPNSKGSDYIEVDYNQPLAMISEKLTLNAVLGHQSYKHNNFLAYTV
                     WKLGATWDFGGGWNAGAYYKGTDAESAAYTVKGKDWSKDRLVGFVTYSFP"
     gene            1751..2089
                     /gene="glnK"
                     /locus_tag="VF309_03695"
     CDS             1751..2089
                     /gene="glnK"
                     /locus_tag="VF309_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7465721.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVTGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAIKTELVDRVIEAIEKSANTGKIGDGKIFVFELEQVVRI
                     RTGETGADAL"
     gene            2107..>2849
                     /locus_tag="VF309_03700"
     CDS             2107..>2849
                     /locus_tag="VF309_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX7465722.1"
                     /translation="MKRSLAHIALATALLVAGAAFAQDKKAEPAPAPAAAAAPADAKA
                     APPAAAAPADAKAAPAAAGAPAAAPAAAPAPTPNKGDTAWMLVSTALVLMMSVPALAL
                     FYGGMVRSKNMLSMLMQVFVVFSLVTVLWCIYGYSIAFTEGNAFFGGFDRIFLKGTFD
                     AMADSQFSLGGTFSKATPMYELVFVVFQATFAAITCCLILGAFAERAKFSAVLLFIVL
                     WFTFSYLPIAHMVWFWPGPDAYTAANVVDA"
BASE COUNT          515 a          937 c          926 g          471 t
ORIGIN      
        1 cgcgatgggc gcggaaggtc atcgcaagcg cgccgcgtat cgggcgcagc tcgccggaca
       61 atgcgagctc gcccgcgaac tcgagctcgc cgaggccccg cgatggaagc tgcccggtgg
      121 cggcgaggat gccgatcgcg atcggaaggt cgaagcgtcc gctttccttg ggaaggtcgg
      181 cgggcgcgag gttcacggtg acgcggcccg cgggaaagtc gaagcgcgcg gtgctcaacg
      241 cggcgcgcac gcgatcgcgc gcttccttca cttcggtttc gggcaggccg acgatatgga
      301 attgcgggag gccgccgccc acgtgcgctt cgacgcgcac cagcggcgca tcgagccctg
      361 cgagaccgcg gctgaagacg acggcgacac tacccacgtt gcgctcctcg atgggaccgc
      421 ccactccctc ggggaacgcg ggccttccgg tgctagggct gcttcgccgc ggcgcgcgct
      481 tcgagctcgg ccacgcgcgt ttcgagcgcg acgagcttct cgcgtgcccg cgagagcact
      541 tgcgtctgca cgtcgtattc ctcgcggctc acgaggtcga ggcgcgtcaa ggcgctcgca
      601 agcaggccgc gcatgttctt ctcgaggtcc ttcgcgggat ttgcggcggc catcgccgcg
      661 aggcgcgcgg cgagctcgct cagcaggttc tcgttcatct cgatcgcttt ctcaacactt
      721 cgatggtcgg tccggttgac ccggaaatgg gcgcacccgg atggtgcggc gcaccttgcg
      781 cgtgcacagg ggctgggcgc ggcgggggcg caacggaatc gagggctgga caaacaatag
      841 cttagccaat ggcacgcttg ctgctattcc cgtccggcaa tcgtcaattc cacagcccat
      901 aaggatacca agatgaaaaa tctgcctaaa gtccttctcc tggccggatg cgcgggactc
      961 gcaatggccg tgggtatcgc acgcgccgaa gacaaacctg ccgacgcggc tgcagcacct
     1021 gccgcaccga cgcccgagca caccttcacg ggcaaggccg cgctctacag cgagtacgaa
     1081 taccgcggca tctcgcagac ctcggagaag cccgcgctgc agctgaacct cgactacgcg
     1141 cacagcagtg gactgtacct cggcacgttc ctgtcgaaca tcaagtggct gaaggacacc
     1201 gcctcggcaa acggcttctc gacgagcgcg ggcatcgaat gggatatcta cgggggctat
     1261 aagttcgagg tggtcaagga cgtgacgctc gacatcggct acctgcgtta cgagtacccg
     1321 agctcgagcg agttctcgcc caagcccaac accgacgagg tctacatcgg cgcctcgtac
     1381 gggccggtgt cggcgaagta ctcgtactcc ttcaacgaca cgttcggggt gcccaacagc
     1441 aagggctcgg actacatcga ggtcgactac aaccagcccc tcgcgatgat cagcgagaag
     1501 ctgacgctca acgccgtcct cggccaccag tcgtacaagc acaacaactt cctcgcgtac
     1561 accgtgtgga agctgggcgc cacctgggat ttcggcggcg gatggaacgc cggcgcgtac
     1621 tacaagggca ccgatgcgga aagcgctgcc tacaccgtga agggcaagga ctggagcaag
     1681 gaccgcctcg tcggcttcgt cacctattcg tttccctgaa cgaagcgcgc acccaaaagg
     1741 agaggaaaaa atgaaactgg tcacggcaat catcaagccg ttcaagctcg acgaggtgcg
     1801 tgaagcgctc tcggcgatcg gcgtgacggg aatcaccgtc acggaagtga aggggttcgg
     1861 ccggcagaag ggccacacgg agctctatcg cggcgcggaa tacgtcgtcg acttccttcc
     1921 gaaggtgaag gtggaggccg cgatcaagac cgagctggtc gaccgcgtca tcgaggcgat
     1981 cgagaagagc gccaacaccg gcaagatcgg cgacgggaag atcttcgtct tcgagctcga
     2041 gcaagtcgtc cgcatccgca ccggcgagac cggcgcggac gcactttgac caaggggaaa
     2101 tcaagaatga aacgctcact ggcccacatc gcgctcgcga ccgcgctcct cgtcgccggc
     2161 gcagcctttg cgcaggacaa gaaggccgaa ccggcgcccg cccccgcggc ggctgccgct
     2221 ccagcggatg cgaaggcggc acctcccgcg gctgccgctc ccgccgatgc caaggcggcg
     2281 cccgccgccg ctggcgctcc cgcagcggca ccggccgcgg cgcccgcgcc gacgcccaac
     2341 aagggcgaca ccgcgtggat gctcgtctcc acggcgctcg tcctcatgat gagcgtgccg
     2401 gccctggcgc tcttctacgg cggcatggtg cgctccaaga acatgctgtc gatgctgatg
     2461 caggtgttcg tggtgttctc gttggtcacg gtgctgtggt gcatctacgg ctacagcatc
     2521 gcgttcacgg aggggaatgc cttcttcggg ggcttcgacc gaatattcct caagggcacc
     2581 ttcgacgcga tggcggactc gcagttctct ctgggaggga ccttctcgaa ggcgacgccg
     2641 atgtacgagc tcgtcttcgt cgtgttccag gcgacgttcg cggccatcac ctgctgcctg
     2701 atcctgggcg ccttcgccga gcgcgcgaag ttctcggcgg tgctgctgtt catcgtcctg
     2761 tggttcacgt tctcgtacct gccgatcgcg cacatggtgt ggttctggcc cggccctgat
     2821 gcgtataccg ccgcgaacgt ggtcgacgc
//
LOCUS       DASVII010000283         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_373422,
            whole genome shotgun sequence.
ACCESSION   DASVII010000283 DASVII010000000
VERSION     DASVII010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373422"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF309_03705"
     CDS             <1..282
                     /locus_tag="VF309_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465723.1"
                     /translation="FAGAPRPDIILLDLNLPKKDGREVLQDIKNDDKLKRIPVVILTT
                     SKAEEDVLKSYNLHANCYVTKPVDLEQFIVVVKSIDMFWLTVVTLPNGK"
     gene            291..>1867
                     /locus_tag="VF309_03710"
     CDS             291..>1867
                     /locus_tag="VF309_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7465724.1"
                     /translation="MNNGDRHSVLLIEDNPGDARLIREMIAEDPEAPFELHCAERLSL
                     GLDFLANRPAGLVLLDLSLPDSLGLETFAKVFAHSPAVPIIVLTGTDDATVALKAVQG
                     GAQDYLVKGKLDRELLVRAMQYSIERKRYQVQIEHQANYDALTGLPNRTLLHDRLRQA
                     VIAQRHVRAIAVVFIDLDHFKFVNDSLGHSAGDELLKQMAERLRSVLRDGDTVARLGG
                     DEFVLILSDQTREDVIFRTMRRLIAKVSDPIMIGERELNITCSAGISLYPQDGPDVQT
                     LLKNADAAMYRAKSQGRNTFQFFTAEMNELANERLSMEQSLRRAIDRDELLLHYQPRV
                     NLATGAVDGVEALVRWNHPVRGLVLPDRFIPLAEETGLIVPIGEWVLRTACEQGEAWR
                     RAGFQPVISVNLSARQLWGGGLVRLVGDVLAATGMADHLELELTESMVMHDAENVIAT
                     MQALKALGVRLSVDDFGTGYSSLSYLKRLPLTALKIDGSFVRDITSGGPDEGLIAKAI
                     ISLGHSLHLKVVAEGVES"
BASE COUNT          330 a          630 c          624 g          283 t
ORIGIN      
        1 ttcgcgggcg cgccgcgccc ggacatcatc ctgctcgacc tcaatctgcc gaagaaggac
       61 ggccgcgagg tgctgcagga catcaagaac gacgacaagc tgaagcgcat cccggtggtg
      121 atcctgacca cttccaaggc cgaggaggac gtactgaaga gctacaacct gcacgccaac
      181 tgctatgtca ccaagccggt ggacctggag cagttcatcg tggtggtgaa atcgatcgac
      241 atgttctggc tcaccgtggt gacgttgccg aacggcaaat aaacgggcaa gtgaacaacg
      301 gcgaccgtca cagcgtactg ctcatcgagg acaacccggg cgacgcgcgc ctgatccgcg
      361 aaatgatcgc ggaggatccc gaggcgccct tcgagctgca ctgcgccgag cggctgtcgc
      421 tcggcctgga cttccttgcc aaccggcccg ccggactggt actgctggac ctgtcgctgc
      481 ccgacagcct gggcctggag accttcgcca aggtgttcgc gcactcgccg gcggtgccga
      541 tcatcgtgct caccggcacc gacgatgcca ccgtcgcgct gaaggcggtg cagggcggcg
      601 cgcaggacta cctggtcaag ggcaagctcg accgcgagct gctggtgcgc gcgatgcagt
      661 actcgatcga gcgcaagcgc taccaggtgc agatcgaaca ccaggccaac tacgacgcgc
      721 tgaccgggct gcccaaccgc acgctgctgc acgaccggct gcgccaggcc gtgatcgcgc
      781 agcgccacgt gcgcgcgatc gcggtggtgt tcatcgacct ggaccatttc aagttcgtca
      841 acgacagcct ggggcacagc gcgggcgacg agctgctcaa gcagatggcc gagcgtctgc
      901 gctcggtgct gcgcgacggc gacacggtgg cgcgcctggg cggcgacgag ttcgtgctga
      961 tcctctccga ccagacgcgc gaggacgtga tcttccggac gatgcggcgc ctcatcgcca
     1021 aggtgagcga cccgatcatg atcggcgagc gcgagctgaa catcacctgc agcgcgggca
     1081 tcagcctcta tccgcaggac ggtcccgacg tgcagacgct cctcaagaac gcggacgcgg
     1141 cgatgtaccg cgccaagtcc cagggccgca atacgttcca gttcttcacc gccgagatga
     1201 acgagctcgc caacgagcgc ctgtcgatgg agcagagcct gcgccgcgcg atcgaccgcg
     1261 acgagctcct gctgcactat caaccgcgcg tgaacctcgc gacgggcgcg gtcgacggcg
     1321 tcgaggcgct ggtgcgctgg aaccacccgg tgcgcggcct cgtgcttccc gaccgcttca
     1381 tcccgctcgc cgaggaaacg gggctcattg ttcccatcgg ggagtgggtg ctgcgcacgg
     1441 cctgcgagca gggcgaggct tggcggcgcg cgggattcca gccggtgatc tcggtgaacc
     1501 tctccgcgcg ccagctatgg ggcgggggcc tggtgcgcct ggtgggcgac gtgctcgcgg
     1561 cgaccggcat ggccgaccac ctcgagctcg agctgaccga gagcatggtg atgcacgacg
     1621 cggagaacgt gatcgcgacc atgcaggcgc tgaaggcgct cggcgtgcgc ctttcggtgg
     1681 acgacttcgg caccggctac tcgagcctgt cgtacctgaa gcgcctgccg ctcaccgccc
     1741 tcaagatcga cggctccttc gtgcgcgaca tcaccagcgg cggccccgac gagggcctca
     1801 tcgccaaggc catcatctcg ctcggccaca gcctgcacct gaaggtggtc gccgagggcg
     1861 tggagtc
//
LOCUS       DASVII010000284         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_696602,
            whole genome shotgun sequence.
ACCESSION   DASVII010000284 DASVII010000000
VERSION     DASVII010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696602"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF309_03715"
     CDS             <1..208
                     /locus_tag="VF309_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465725.1"
                     /translation="TGPITLTISGTSAALLARLASDPSLGGDAGAVMMRALGLFDLAL
                     KAKREGKKLGFYDPERDTLSEVAF"
     gene            324..1430
                     /locus_tag="VF309_03720"
     CDS             324..1430
                     /locus_tag="VF309_03720"
                     /inference="COORDINATES: protein motif:HMM:NF003711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX7465726.1"
                     /translation="MSLRIDQDHRRFREIVRGKIKQNLRKYITQGEMLGKRGKELVSI
                     PLPQIDIPHFRYGPKQSGGVGQGTGNPGDALGQGPPQPGQGKAGDRPGEHLLEVDVTM
                     EELAQILGEELRLPRIEPKGKERIVARKDRYTGVRNTGPESLRHFRRTFKQALRRQIA
                     SGTYNPKNPLIVPVREDKRYRSWRSDPLPQSNAVIIYMMDVSGSMGDEQKEIVRIESF
                     WIDTWLRSQYQGIESRYIIHDAMAKEVDRETFFRTRESGGTMISSAYKLCSKLITEEY
                     PPSEWNIYPFHFSDGDNWSVDDTVTCIEILKSNILPSVNLFCYGQVESPYGSGQFIKD
                     LQEHFDDDGNLTTSEIKGKESIMDSIRDFLGKGK"
BASE COUNT          297 a          461 c          511 g          251 t
ORIGIN      
        1 caccgggccc atcacgctca ccatcagtgg cacgtcggcg gcgttgctgg ctcggcttgc
       61 atcggatccg tccctgggcg gcgatgcggg cgcggtcatg atgcgcgccc tgggtctctt
      121 cgatctggcg ctcaaggcca agcgcgaagg caagaagctc ggcttctacg atcccgagcg
      181 cgacacgctg agcgaggtcg cgttctagat gagccgcctg gccggaggtc catatcgatg
      241 agcgagcgag agcgagcgaa tttgagcgag gcccgtaggg cccgcggatc gactgcttgc
      301 tcctggccgc acggagtgcg gacatgagtc tgcggatcga tcaagatcac cgccgcttcc
      361 gcgagatcgt gcgcggcaag atcaagcaga acctgcgcaa gtacatcacg cagggggaga
      421 tgctcggcaa gcgcggcaag gagctcgtct ccatcccgct gccgcagatc gacatcccgc
      481 actttcgcta cggccccaag cagtcgggcg gcgtcggcca gggcaccggc aaccccggcg
      541 acgccctcgg gcagggcccg ccgcagccgg gccaaggcaa ggcgggcgat cggccgggcg
      601 agcatctgct cgaggtcgac gtgacgatgg aggagctggc gcagatcctc ggcgaggagc
      661 tgcggctgcc gcgcatcgag cccaagggca aggagcgcat cgtcgcgcgc aaggatcgct
      721 acaccggcgt gcgcaacacc gggcccgagt cgctgcgcca cttccggcgc acgttcaagc
      781 aggcgctccg gcggcagatc gcgtcgggca cgtacaatcc gaagaacccg ctcatcgtgc
      841 cggtgcgcga ggacaagcgc tatcgctcgt ggcgatcgga tccgctgccg cagtcgaacg
      901 ccgtcatcat ctacatgatg gacgtgtcgg gctcgatggg cgacgagcag aaggagatcg
      961 ttcgcatcga gagcttctgg atcgatacct ggctgcgctc gcagtatcag gggatcgaga
     1021 gccgctacat catccacgac gcgatggcca aggaggtcga tcgcgagacc ttcttccgca
     1081 cgcgcgagag cggcggaacg atgatttcct cggcatataa gctgtgcagc aaattgatta
     1141 ccgaggaata tccgccgagc gaatggaaca tctatccgtt ccatttctcc gacggtgaca
     1201 attggtcggt cgatgacacg gtgacgtgca tcgaaatcct gaaatcaaat attctgccca
     1261 gcgtaaacct gttttgctat gggcaggtgg aatcaccgta cggctcggga cagttcatca
     1321 aggacctgca ggagcatttc gacgacgacg gcaatctgac caccagtgag atcaagggaa
     1381 aggagtcgat catggactcg atccgcgatt tcctcgggaa gggcaagtaa tgctgccggt
     1441 agttgcgaag gtcgcgagcg cgcgcgggtt gcgcgcgggt gcgttcgtcg ccgcggcgat
     1501 ttgcgtcggc ggcgcgctgg
//
LOCUS       DASVII010000285         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_49507, whole
            genome shotgun sequence.
ACCESSION   DASVII010000285 DASVII010000000
VERSION     DASVII010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49507"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VF309_03725"
     CDS             complement(<1..976)
                     /locus_tag="VF309_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7465727.1"
                     /translation="MAGASGPYDALIVGGGHNGLVCASYLAAGGLRVCVVEKRAAVGG
                     AAVTEEFHPGFRNSTASYTVSLLNPKVIRDLRLADHGLRIVERPFANFLPLPDGRFIK
                     VGGGVEATQREIAKFSKRDAERLPEYYARLEVVSDVLRGLLLETPPNVGGGVAEIFSA
                     WKAGRRLNRLPLAAKRDVLDLFTVSATEWLERWFESDPIKACFGFDAVVGNFASPHSP
                     GSAYVLLHHVFGEVNGKMGSWGHAIGGMGAITQAMRLEAEARGVEIRTGKGVARILVR
                     EGRARGVALDDGAQIAARCVVSNAHPRLLLDLLGEEGGEWREAFGHYQS"
     gene            complement(981..1817)
                     /locus_tag="VF309_03730"
     CDS             complement(981..1817)
                     /locus_tag="VF309_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7465728.1"
                     /translation="MREEQVRCLDPHGFHFMRYTEWGDAKNPRVLLCVHGLTRNARDF
                     DFIAERLSDAYRVVCVDIAGRGRSDWLRDPSDYTYAVYGGDVTTLIASLHAETVDWLG
                     TSMGGIIGMIMASLPGTPVRKLAMNDVGLLIPRASLVRIGEYVGREATYDSLDALESA
                     MRGVSPFGNLDGAQWRHLALHVAKQDEDGRWKFRYDPGIGKNFHAAPPVDIDLRPFWN
                     AVRGPALVIRGEHSDLLLPETLEEMRMRPHTETHVVPDTGHAPMLMDDSQVGVVRRFL
                     LG"
BASE COUNT          342 a          673 c          653 g          324 t
ORIGIN      
        1 ccgattgata atggccgaag gcttcgcgcc attcgccgcc ctcctcgccg aggagatcga
       61 gcagcaggcg cggatgcgcg ttcgagacca cgcagcgggc ggcgatctgc gcgccatcgt
      121 cgagtgccac gccgcgcgcg cgaccctcgc gaacgaggat gcgcgccacg cccttccccg
      181 tgcgaatctc tacgccgcgc gcttcggcct cgagccgcat ggcctgtgtg atcgcgccca
      241 tgccgccgat cgcgtggccc cagctgccca tcttcccgtt gacctcgccg aacacgtgat
      301 gcagcagcac ataagcggat cccggggaat ggggactcgc gaaattcccg acgacggcat
      361 cgaaaccgaa gcacgccttg atgggatcgg actcgaacca gcgctcgagc cattcggtgg
      421 cggagaccgt gaacaggtcg agcacatcgc gcttcgccgc gagcggcaat cgattcagcc
      481 gccttcccgc cttccatgcg ctgaagatct cggcgactcc gccgccgacg ttggggggcg
      541 tctcgagcag gaggccgcgc agcacgtcgg agacgacttc gagcctcgcg tagtactccg
      601 gcaagcgctc cgcgtcgcgc ttcgagaatt tcgcgatctc gcgctgcgtg gcttccacgc
      661 cgccgccgac cttgatgaac ctgccgtcgg gcagcggcag gaaattcgcg aacggccgct
      721 cgacgattcg caggccgtgg tccgcgagcc gcaggtcgcg aatgaccttg ggattgagga
      781 ggctgaccgt gtagctcgcg gtcgagttgc gaaagcccgg atggaattcc tcggtgaccg
      841 cggcgccgcc cacggcggcg cgcttctcca cgacgcaaac gcgcaagccc cccgcggcca
      901 ggtacgacgc gcagacgagc ccgttatgac cgcccccgac gatcagcgcg tcgtagggcc
      961 cggatgcacc cgccatcgca tcagccgagc aggaagcggc gcaccacgcc gacctgcgaa
     1021 tcgtccatca gcatcggcgc atggcccgtg tcgggaacga cgtgggtctc cgtatggggg
     1081 cgcatgcgca tctcctcgag cgtctccggc aggagcaggt ccgagtgctc gccgcggatc
     1141 acgagcgccg gcccgcgcac ggcgttccag aaggggcgca ggtcgatgtc cacgggcggc
     1201 gccgcgtgga agttcttgcc gatgcccggg tcgtagcgga atttccagcg gccgtcctcg
     1261 tcctgcttcg cgacgtgcag cgcgagatgg cgccattgcg cgccgtcgag gtttccgaac
     1321 gggctcacgc cgcgcatcgc gctctcgagc gcgtccaggg aatcgtaggt cgcctcgcgg
     1381 cccacgtact cgccgatgcg caccagcgac gccctgggaa tgagcaggcc cacgtcgttc
     1441 atcgcgagct tgcgcaccgg cgtccccgga aggctcgcca tgatcatgcc gatgatgccg
     1501 cccatggagg tgccgagcca atcgaccgtc tcggcgtgga ggctcgcgat gagggtggtc
     1561 acgtcgccgc catagacggc atacgtgtag tccgatggat cgcgcagcca gtcgctcctc
     1621 ccgcgcccgg cgatgtcgac gcacacgacg cggtaggcgt ccgagagcct ctcggcgatg
     1681 aaatcgaaat cgcgcgcgtt gcgcgtgagg ccgtgcacgc aaagcagcac tcgcgggttc
     1741 ttcgcgtcgc cccactcggt atagcgcatg aagtggaacc cgtgcggatc caggcagcgc
     1801 acctgttctt cgcgcatggt ggtcatggat ggagaaaggc tttgtatccg cagataaacg
     1861 cagatgaacg cagatgagcc tctcgatatt gtgtcttgag caccgttgcg accatcgtca
     1921 aagtgagcac gaatgactcg taggatgttc gggggttaac cgcgttcatc tgcgtttatc
     1981 tgcggatcca aa
//
LOCUS       DASVII010000286         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_265189,
            whole genome shotgun sequence.
ACCESSION   DASVII010000286 DASVII010000000
VERSION     DASVII010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265189"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /gene="gshA"
                     /locus_tag="VF309_03735"
     CDS             <1..1245
                     /gene="gshA"
                     /locus_tag="VF309_03735"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX7465729.1"
                     /translation="ESLPAIERWFRLEWMEHTPPFYTSVDLRNAGFKLAPVDTNLFPG
                     GFNNLNPEFLPLCVQAAMSAVQRICPDTRRFLIVPENHTRNLHYLQNVAVLRRILEGA
                     GFEVRIGSLIPELSSPMEVEASGGDKLLLEPLQRRGNRIGVEGFDPCAVLLNNDLSAG
                     IPAILRGIEQPVVPPLVSGWSTRRKSHHFHVYNRVAEDFAKLIGIDPWVINPAFSQCG
                     KVNFNEKEGEDCLAANVEYVLNEVREKYAHYGITEQPFCIVKADAGTYGMGIMTVKSV
                     DDVRNLNRKTRNKMSVIKEGQPVHEVIIQEGVYTFESVDGAVAEPVVYMMDQFVVGGF
                     YRVHTERGIDENLNSPGAKYVPLAFETNCHTPDCAGKPGDPPNRFYTYGVVARLANLA
                     AAIEVEELVERNSALEVRAANA"
     gene            1242..2213
                     /gene="gshB"
                     /locus_tag="VF309_03740"
                     /pseudo
     CDS             1242..2213
                     /gene="gshB"
                     /locus_tag="VF309_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014947.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
     gene            complement(2210..>2664)
                     /locus_tag="VF309_03745"
     CDS             complement(2210..>2664)
                     /locus_tag="VF309_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465730.1"
                     /translation="FNYTVEAWTLVDEMLGAVRVQRPATASKGIAIGRLVAIRMGDAG
                     PFYLGALSELASETDGRIIATVTLFPGKPETICVRAGDARNRANAHWTQGLSLPALEK
                     LNIPSSLVVPSGMGTRGRGIEMWSEGVRESTVEEVLQRGADFDRVTTF"
BASE COUNT          458 a          905 c          891 g          410 t
ORIGIN      
        1 gagtcgctgc cggcgatcga gcgctggttt cgcctcgagt ggatggagca cacaccgccg
       61 ttctacacgt cggtcgatct gaggaacgcc ggcttcaagc tcgcccccgt cgacaccaac
      121 ctctttcccg gcggcttcaa caatctgaat ccggagttcc tgccgttatg cgtgcaggcg
      181 gcgatgtccg cggtgcagcg catctgtccc gacacgcggc gcttcctcat cgtcccggag
      241 aaccacacgc gcaacctgca ctacctgcag aacgtggcgg tgctgcgccg catcctcgag
      301 ggcgcgggat tcgaggtgcg gatcggaagc ctcattccgg agctctcctc gccgatggag
      361 gtcgaggctt cgggcggcga caagctcctg ctcgagccgc tgcaacggcg cggcaaccgc
      421 atcggcgtcg agggcttcga tccttgcgcg gtgcttctca acaacgacct gtccgcgggc
      481 atccccgcga tcctgcgggg catcgagcag ccggtcgtgc cgcccctggt ctccggatgg
      541 tccacgcgcc gcaagtcgca tcacttccac gtctacaacc gggtcgccga ggacttcgcg
      601 aagctgatcg gcatcgatcc gtgggtgatc aaccccgcct tcagccaatg cggcaaggtg
      661 aacttcaacg agaaggaagg cgaggattgc ctcgccgcga acgtggagta cgtcctcaac
      721 gaggtgcgcg agaagtacgc gcactacggc atcaccgagc agccgttctg catcgtgaag
      781 gccgacgcgg gcacctacgg catgggcatc atgaccgtga agagcgtcga cgacgtgagg
      841 aacctcaatc gcaagacgcg caacaagatg tcggtgatca aggaagggca gccggtccac
      901 gaggtgatca tccaggaagg cgtctacacc ttcgagagcg tcgacggcgc cgtcgccgag
      961 ccggtcgtct acatgatgga ccagttcgtg gtgggcggct tctatcgcgt gcacaccgag
     1021 cgcggcatcg acgagaacct gaactcgccg ggcgccaagt acgtgccgct cgccttcgag
     1081 accaactgcc acacgccgga ttgcgcggga aagcccggcg atccgcccaa tcgcttctac
     1141 acctacggcg tggtggcgcg cctcgccaac ctcgccgccg cgatcgaggt cgaggagctc
     1201 gtcgagcgca attccgcgct ggaggtgagg gcggcgaacg catgaagctc gcgttcgtgc
     1261 tggacccgct ggaaaccctc aaggcctaca aggactcgag cgtcgcgatg atgcgcgagg
     1321 cgcggcgccg cgggcacgag gtgcacgcgc tcgaggcgcg ctccatgtat ttgcgctagg
     1381 gccgagtgat ggcctcggtg cgcaagctct cggtgagcga cgacgaccac gcgtggtacc
     1441 gcgccgacgc ggcgcgcgac gacgtgcttt ccgggttcga cctcgtcctg atgcgcaagg
     1501 acccgccgtt cgacctcgag tactacaacg cgacgctcct cctcgatcgc gtcgaggccg
     1561 agggcgcgtg cgtcgtgaac cggccgcgcg cgcttcgcga ctggaacgag aagctgggga
     1621 tcctcgcctt cgcgtccctc gcgccgccga cgctcgtcgc gagcgagatg gcgcgcatcc
     1681 acgccttcat cgacgagcac cgcgacgtcg tcgtgaagaa gctggacggc atgggcgggg
     1741 ccatgatctt ccgcgtgaaa tccgacgacg tgaaccgcaa cgtgatcgtc gaggcgatca
     1801 ccgacctggg cgctcgcacc gtgatggcgc agcgctacat ccccgagatc gcgaagggcg
     1861 acaagcgcgt gctggtgatc gacgggaaac ccttctccca ttgcctcgcg cgcatcccga
     1921 aggcgggcga gacgcgcggc aacctcgccg ccgggggccg gggcgtggcg cagccgatct
     1981 cggcgcgcga cctcgagatc gccgagacga tcgggccgca cctggtgaag gcgggcatcg
     2041 tcttcgcggg gctcgacgtg atcggcgatt gggtgacgga gatcaacgtg acgagcccga
     2101 cctgcatcgt cgagatcgcg cagcagaccg ggcgcaacgc cgcggtggac ctgctcgacg
     2161 ccctggagcg cgctcgcgca gcgaccccct ccccgaccgc gaatcgctcc tagaaagtcg
     2221 tgacgcggtc gaagtcggcg ccgcgctgga gcacttcctc caccgtcgat tcccggacgc
     2281 cctcgctcca catctcgatg ccgcggcccc gcgtgcccat gccgctggga accacgaggg
     2341 aggaaggaat gttgagcttc tcgagcgcgg gaagcgaaag cccctgcgtc cagtgcgcgt
     2401 tggcgcgatt gcgcgcgtcg cccgcgcgca cgcagatggt ctcgggcttg cccggaaaga
     2461 gcgtaaccgt ggcgatgatg cgcccgtcgg tctcgctggc gagctcgctc agcgcgccga
     2521 ggtagaaggg cccggcatcg cccatgcgga tggcgacgag gcgcccgatc gcgatcccct
     2581 tcgacgcggt cgccgggcgc tgcacgcgaa ccgcgcccag catctcgtcg acgagcgtcc
     2641 atgcctcgac cgtgtagttg aagt
//
LOCUS       DASVII010000287         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_624730,
            whole genome shotgun sequence.
ACCESSION   DASVII010000287 DASVII010000000
VERSION     DASVII010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624730"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VF309_03750"
     CDS             complement(<1..410)
                     /locus_tag="VF309_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEX7465731.1"
                     /translation="MTPLREQVAREGFALVGAARMRPLLGAADALPDWDRFAASWNDL
                     PLDTQLPEGHRYRRRRHATLSARAGESGWRLEAHQPHYQSLDYNPLVGGIERWFEPIR
                     PEIVEGASFDRIVGFCLRLFGPMRPDADWHIECH"
     gene            complement(460..900)
                     /locus_tag="VF309_03755"
     CDS             complement(460..900)
                     /locus_tag="VF309_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465732.1"
                     /translation="MRNILATTTAAFAFAFACGSATAAPMTKDEYKAAKKKILADYTV
                     ERQKCGSRGGNALALCVAHAHGERDVAKAELEAQYKPSARTNYNAAIARSKSAYTIAR
                     DECNEHSGPEKKACLKEAVDVRKAAEADALAKRTAQRAEEAAKH"
     gene            982..>1882
                     /locus_tag="VF309_03760"
     CDS             982..>1882
                     /locus_tag="VF309_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7465733.1"
                     /translation="MRSLLALAALCLPVTAAFAHAPNEPPHQLFALGDFKLESGEAIR
                     DFSVSYVTHGTLDASKSNAILMVTAIGGNHHRIDHLIGPGKALDTSKYFVIAVDAIAN
                     GLTTSPSNSRSQPGMQFPKFNIRDMVNSAQRLVRDKFGIEKLVTVVGASMGGMQALQF
                     AVSYPDMTRSVVAIVPLYRTTPWTTDVLELLRQGIMTDAAWNNGNYTAEAPPEKGMRL
                     WSRMLNGVIVRTPDALQADFPKSNMDVIAWQQGLADSGWKRMDARDWIYQSWAYDQHN
                     VGTTPGFNGDHERALKSIKARTLI"
BASE COUNT          325 a          636 c          614 g          307 t
ORIGIN      
        1 tggtggcact cgatgtgcca atcggcgtcg gggcgcatcg gaccgaagag ccgcaggcag
       61 aatccgacga tgcgatcgaa cgacgcgccc tcgacgatct cggggcgaat cggctcgaac
      121 caccgctcga tgccgccgac gagcgggttg tagtcgaggc tctgatagtg gggctgatgc
      181 gcttcgaggc gccatccgct ctcccctgcg cgcgcggaca gcgtcgcgtg gcgacgtcgc
      241 cgatagcgat ggccttcggg aagctgcgtg tcgaggggaa gatcgttcca gctcgcggcg
      301 aagcggtccc aatccggcag cgcatcggcg gcgcccagca gcgggcgcat gcgcgccgcg
      361 cccacgagcg cgaacccctc ccgcgcgacc tgctcgcgca agggagtcat tccgacgatc
      421 gaatcgggag gcattggagc gaaagcgaat cgaatgcggc tagtgcttcg cggcctcctc
      481 ggcgcgctgc gcggtgcgct tcgccagcgc atcggcctcg gcggccttgc gcacgtcgac
      541 ggcttccttg aggcaggcct tcttttccgg gccgctgtgc tcgttgcact cgtccctcgc
      601 gatcgtgtac gcggacttcg atcgcgcgat ggcggcattg tagttggtgc gggcgctggg
      661 cttgtactgg gcctcgagct cggccttcgc cacgtcgcgt tcgccatggg catgggcgac
      721 gcacaaggcg agcgcgttgc cgccccgcga gccgcacttc tggcgctcga ccgtgtagtc
      781 ggcgaggatc ttcttcttcg cggccttgta ctcgtccttc gtcatcggcg cggccgtcgc
      841 gctgccgcac gcgaaggcga aggcgaatgc ggctgtcgtg gttgcaagga tgttgcgcat
      901 ggagatctcc tggtcgagcg gggatgatgc atgataaccg cagcgccaga cgccacgaaa
      961 aacagcctcc ggaggatgtc catgcgatcc ctgctcgccc ttgccgcact ctgcctgccc
     1021 gtcaccgccg ccttcgccca cgcgcccaac gagccgcccc accagctctt tgcgctcggc
     1081 gacttcaagc tggagagcgg cgaggcgatc cgcgatttct cggtctcgta cgtgacgcac
     1141 ggaacgctcg acgcatcgaa gtccaacgcg atcctgatgg tcaccgcgat cggcggaaac
     1201 caccaccgca tcgatcacct gatcgggccc ggcaaggcgc tcgacacctc gaaatacttc
     1261 gtgatcgcgg tggatgcgat cgccaacggc ctcaccacct cgccctcgaa cagcaggagc
     1321 cagccgggaa tgcagttccc gaaattcaac atccgcgaca tggtgaattc ggcgcagcgc
     1381 ctggtgaggg acaagttcgg catcgagaag ctcgtcaccg tcgtcggcgc ctccatgggc
     1441 ggcatgcagg cgctgcagtt cgccgtgagc tatcccgaca tgacgcgaag cgtcgtcgcg
     1501 atcgtgcccc tctaccggac gacgccatgg accaccgacg tgctcgagct cctgcgccag
     1561 gggatcatga ccgacgccgc gtggaacaat ggcaactaca ccgcggaagc gccgcccgag
     1621 aaaggcatgc gcctctggtc gcgcatgctg aacggcgtga tcgtgcgcac gcccgacgcg
     1681 ctgcaggccg acttccccaa gagcaacatg gatgtgatcg cgtggcagca gggcctcgcc
     1741 gacagcggct ggaagcgcat ggacgcgcgc gactggatct accaaagctg ggcctacgac
     1801 cagcacaacg tggggaccac tcctggattc aacggcgacc atgagcgcgc gctgaaatcc
     1861 atcaaggcgc gaacgctgat cc
//
LOCUS       DASVII010000288         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_121773,
            whole genome shotgun sequence.
ACCESSION   DASVII010000288 DASVII010000000
VERSION     DASVII010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121773"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..814)
                     /locus_tag="VF309_03765"
     CDS             complement(155..814)
                     /locus_tag="VF309_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX7465734.1"
                     /translation="MDGARDVFMTGATGYMGRALAARLIARGHRVRALVRPGSESRLP
                     AGCEPVRGDALDASTFAAGIRAADTFVQLVGVAHPSPAKAAQFRSIDLASARAGIEAA
                     RAAGVMHFVYVSVAHPAPVMKAYIAARTEAEALLRASALPATILRPWYVLGPGHRWPV
                     VLAPAYWILERFAPTREIARRLGLVTLEQMVAALAASVENPASGVRVLEVADIRRAAT
                     A"
     gene            862..1833
                     /locus_tag="VF309_03770"
     CDS             862..1833
                     /locus_tag="VF309_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7465735.1"
                     /translation="MNYRRLGRSGLKVSELSLGSWVTYGNQLDTGAARECMAAAWDRG
                     VNFFDNAEVYAAGKSEEIMGEALKKLAWPRLKYIVSTKFFWGITEGPNEKSTLNRKYL
                     MNAIDGSLARLQLDYVDLVYCHRADPSTPMEETVWAMHDMIDRGKALYWGTSEWSAAQ
                     IVEAWQVADRHHLRKPVMEQPEYNLFHRSRVEREYAKLYDDIGLGLTTWSPLASGLLT
                     GKYRAGVPSDSRGAMPRMKFLVEGLTDKKRNDAVAKLESVAKDLGCSLAQLAIAWCAR
                     NPHVSTVITGASKVSQVEENLRAADFIEKLTPEVMQRIAGLTAALAE"
     gene            complement(1836..2723)
                     /locus_tag="VF309_03775"
     CDS             complement(1836..2723)
                     /locus_tag="VF309_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HEX7465736.1"
                     /translation="MTPPQPISLDHLPSRHAFEADPPEHLFHYTDLEGVKGIFTSRTL
                     WMSKFTASNDISEILLAIEHFQSFVARKAAEVTKDEGDFLREAADQLESFRRTNICVA
                     SFCEAPDLLSQWRSYGNDGRGIALGFNSKRLVELAQRHGLRLFRCVYDQAAHERIASD
                     LVDSLLASFRASPPDGPQARRALITQFNSMFLVVAPVIKDHRFVEEREWRLVSNPVPF
                     DDPNMIAVLKRNQASVKLTVKLTGDSEGMSNMVPRVTIGPTLDPHNVSDAIDVLSQRN
                     GFHISDIAISGIPYRRVAE"
     gene            complement(2757..>3148)
                     /locus_tag="VF309_03780"
     CDS             complement(2757..>3148)
                     /locus_tag="VF309_03780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7465737.1"
                     /translation="GDVLVIRYEGPKGGPGMREMLAPTSALIGKGLGDSVGLITDGRF
                     SGGTWGMVVGHVTPEAFEGGAIALVNEGDSITIDAPRRIVQLNVPDAEIAKRRAQWKQ
                     PKPRYTTGVLGKYAKLVGTASLGAVTD"
BASE COUNT          509 a         1052 c         1110 g          477 t
ORIGIN      
        1 gcgcgagcgc ggcgttcatc gtcgcgctca cgggatgggg caacgaagcc gacaaggatc
       61 gggcgcgggc cgccggattc gatcgccacc tggtgaagcc ggtggatccc gcgcagctgc
      121 tggagatcct cgccgccgtt ccggcgttga agccctaggc cgtcgccgcg cggcggatat
      181 cggcgacttc gagcacgcgc accccgctcg cgggattctc gaccgacgcc gcgagcgccg
      241 cgaccatctg ctcgagcgtg acgaggccca gccggcgggc gatttcgcgc gtgggcgcga
      301 agcgctcgag gatccaatag gcgggcgcga ggaccaccgg ccagcgatgg cccggcccga
      361 ggacgtacca cggccgcagg atcgtggcgg gaagggcgct cgcgcgcagc agcgcctcgg
      421 cttcggtgcg cgccgcgatg tacgccttca tcaccggcgc cggatgcgcg acgctcacgt
      481 agacgaagtg catcacgccg gcggcgcgcg ccgcctcgat acccgcgcgc gccgaggcga
      541 ggtcgatgct gcggaattgc gcggctttcg ccgggctcgg gtgcgcgacg ccgacgagct
      601 gcacgaaggt gtcggcggct cgaatgcctg ccgcgaaggt ggaggcgtcg agcgcatcgc
      661 cgcggaccgg ctcgcaaccc gccggaaggc gcgactcgga tccgggccgc accagggcgc
      721 gcacgcgatg cccccgcgcg atgagccgcg ccgcgagcgc gcggcccatg tagcccgttg
      781 ccccggtcat gaaaacgtcg cgcgcgccgt ccatcgaacc gctatgctac gcgtcccggc
      841 atttccaata ttcgaagagc gatgaactat cgacgtctcg gccgctccgg cctcaaggtg
      901 agcgagctct cgctcggctc gtgggtgacc tacggcaacc agctcgacac cggcgccgcg
      961 cgcgaatgca tggccgccgc gtgggaccgc ggcgtgaact tcttcgacaa cgcggaggtg
     1021 tacgccgcgg gcaagagcga ggagatcatg ggcgaggcgc tgaagaagct cgcgtggccg
     1081 cgcttgaagt acatcgtctc gaccaagttc ttctggggaa tcaccgaggg gcccaacgag
     1141 aaatcgacgc tcaatcgcaa gtacctgatg aacgcgatcg acggctcgct cgcgcgcctg
     1201 cagctcgatt acgtggatct cgtctattgc caccgcgccg atccctccac gccgatggag
     1261 gagaccgtgt gggcgatgca cgacatgatc gaccgcggca aggcgctcta ctggggcacc
     1321 tcggagtgga gcgcggcgca gatcgtggag gcgtggcagg tcgccgatcg ccaccacctg
     1381 cgcaagcccg tgatggagca gcccgagtac aacctgttcc atcgaagccg cgtggagcgc
     1441 gaatacgcga agctctacga cgacatcggc ctggggctca cgacgtggag cccgctcgcc
     1501 tcggggctgc tgaccggaaa atatcgcgcg ggtgttccct ccgacagccg gggcgcgatg
     1561 ccgcgcatga agttcctcgt cgagggcctc acggacaaga agaggaacga cgccgtcgcg
     1621 aagctcgagt cggtcgccaa ggacctgggc tgctcgctcg cgcagctcgc catcgcgtgg
     1681 tgcgcgcgca atccccatgt ctcgaccgtg atcaccggcg cgtccaaggt gtcccaggtc
     1741 gaggagaacc ttcgggcagc ggacttcatc gaaaagctca cccccgaagt gatgcagcgc
     1801 atcgcggggc tcacggccgc cctggcggag tagttctact cggcgacgcg ccggtacggg
     1861 attcccgaga tcgcgatgtc cgagatgtgg aagccgttgc gctgcgagag gacgtcgatc
     1921 gcgtccgaga cgttgtgcgg atcgagcgtg ggcccgatgg tcacgcgcgg gaccatgttg
     1981 gacattccct ccgaatcgcc ggtgagcttc accgtgagct tcaccgacgc ctggttgcgc
     2041 ttcagcacgg cgatcatgtt gggatcgtcg aagggcaccg ggttcgagac gaggcgccac
     2101 tcgcgctcct cgacgaagcg gtggtccttg atcacgggcg ccaccaccag gaacatcgag
     2161 ttgaactgcg tgatgagggc gcggcgcgcc tgcgggccgt cgggcggcga tgcgcggaag
     2221 gacgcgagca gcgaatcgac gaggtcgctc gcgatcctct cgtgcgcggc ctggtcgtac
     2281 acgcagcgga agagcctcag gccgtggcgt tgggccagct cgacgaggcg cttggagttg
     2341 aagccgagcg cgatgccgcg cccgtcgttg ccgtaggagc gccactggct gaggaggtcc
     2401 ggcgcctcgc agaagctcgc gacgcagatg ttggtgcgcc ggaagctctc gagctggtcc
     2461 gcggcctcgc gcaggaagtc gccctcgtcc ttcgtgacct ccgcggcctt gcgcgcgacg
     2521 aagctctgga agtgctcgat cgcgagaagg atctcggaga tgtcgttgga cgcggtgaac
     2581 ttcgacatcc acagcgtgcg cgacgtgaag atgcccttca cgccctcgag atcggtgtag
     2641 tggaagagat gctcgggggg atcggcctcg aaggcgtgcc gcgagggcaa atggtccagg
     2701 ctgatcggct gcggtggcgt cacgcggacg gtgtccccgg cgtcactcga tcttcatcag
     2761 tcggtcacgg cgcccaggct cgcggtgccg acgagcttcg cgtacttgcc gagcacgccg
     2821 gtcgtgtacc tgggcttcgg ctgcttccat tgcgcgcgcc gcttcgcgat ctccgcgtcc
     2881 ggcacgttca gctgcacgat gcgccggggc gcgtcgatgg tgatcgagtc gccctcgttc
     2941 accagcgcga tcgcgccgcc ctcgaacgcc tcgggagtca cgtggccgac gaccatgccc
     3001 cacgtgcctc cggagaagcg cccgtcggtg atgagcccca cggagtcccc gagccccttg
     3061 ccgatgagcg ccgacgtggg cgcgagcatc tcgcgcatgc cgggcccgcc cttggggccc
     3121 tcgtagcgga tcaccagcac gtcgcccg
//
LOCUS       DASVII010000289         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_193179,
            whole genome shotgun sequence.
ACCESSION   DASVII010000289 DASVII010000000
VERSION     DASVII010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193179"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..868
                     /locus_tag="VF309_03785"
     CDS             98..868
                     /locus_tag="VF309_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465738.1"
                     /translation="MACFVSLAALGVHGQARAPASDGFRLTTAMHLSTLAERIAKLHA
                     QVGQGILPERGRKALHEAIREFDATLRIVSARASTAEGRDNYALLALLWQDYRDWATR
                     TPSRESARKLRPRTEEVLWVAVKGARMLQADARTVASASAVRAEGAATLAQRIAKLYL
                     WSRWDIHDEALDRELRESEDNLRRALDFLRTAPDNTPAIADELQAADIQLRFMEDAAR
                     ALKARDTTARHIEFIAKTGDHIQESMERVVSLYENAAR"
     gene            complement(1362..>1745)
                     /locus_tag="VF309_03790"
     CDS             complement(1362..>1745)
                     /locus_tag="VF309_03790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7465739.1"
                     /translation="VAVDRVGRSGGGALAQAAKQKLVDMGADLVKSGFILRPLDRALE
                     YEADRMAIVIAARSGYDPYGLVAVLQMLAQAHGDGDGASVFATHPAPGDRITELERLG
                     PSVFDRYGAQPQVEGRFKQVVGAAK"
BASE COUNT          269 a          602 c          605 g          269 t
ORIGIN      
        1 tgtcgcttgc gcgattcgtc tcggaagggg tgatcgcggg gttcacgccg cccgccgcag
       61 gcgcgtgagg gccgcggcgg ccgcttcgcg cctcgccgtg gcctgcttcg tatcgctcgc
      121 tgcgctcggc gtccatgggc aggcgcgggc gcccgcgagc gacgggtttc gcctcaccac
      181 cgcgatgcac ctgtccacgc tcgccgagcg catcgcgaag cttcacgcgc aggtgggaca
      241 gggcattctt cccgagcgcg gccgcaaggc gctccacgag gcgattcgcg aatttgacgc
      301 gacgctgcga atcgtctcgg cgcgcgcgtc caccgcggaa ggccgcgaca actacgcgct
      361 gctcgccctg ctgtggcagg actaccgcga ttgggcgacg cgcaccccga gccgcgaaag
      421 cgcgcgcaag ctgaggccgc gcacggagga agtgttgtgg gtcgcggtga agggcgcgcg
      481 gatgctgcag gcggatgcgc gcaccgtcgc cagcgcttcg gcggttcgcg ccgagggtgc
      541 cgcgacgctc gcgcagcgca tcgcgaagct gtacctgtgg tcgcgctggg acatccacga
      601 cgaggcgctc gaccgcgagc tgcgcgagtc ggaggacaac ctgcgtcgcg cgctcgactt
      661 cctgcgcacc gcgcccgaca acacgcccgc catcgccgac gagctgcagg ccgctgacat
      721 ccagctgcgc ttcatggagg acgccgcgcg ggcgctgaag gcgcgcgaca ccacggcgcg
      781 gcacatcgag ttcatcgcga agacgggcga ccatatccag gagtcgatgg agcgcgtcgt
      841 ctcgctctac gagaacgcgg ctcgctagtt atctcaacac tccggtgatc ggccaggcgt
      901 ggaaggggcg gttcttctcg acccggggga cctcggcttc tctacgggga gagatctcgc
      961 ttcaacccat tgcgctcgaa ttccttgcgc ttcttctcat ctgcggcaag cggggcaccc
     1021 cactctcgag aagaaccgca acccttccac gccaggggtt gatcgagagg cgtcgctact
     1081 gctagcggga tgcgcagtgc ctgttcgaac tgcatcgacc cggtgaatgc cgccccgatg
     1141 ggaaccaccg agtagggctt ggggaaaagc tcgagagggc gccaggtgat tgtgaaaagg
     1201 gccgctgccg ccacgcatcg tcactagagt gtcgacgcgc gtcaccgccg cccgggtcga
     1261 gcttttcccc gccctagctc ggtggtgatc gcaccaccgg gtgcgcgcgg tgaatacccc
     1321 ttgaatccca cgctcggact tgcggctgtc aaacgcgacg gctacttcgc ggcgccgacg
     1381 acctgcttga agcggccctc gacttgcggc tgggcgccgt agcggtcgaa taccgagggg
     1441 ccgaggcgct cgagctcggt gatgcgatcg cccggcgcgg gatgggtcgc gaagaccgac
     1501 gcgccatcgc cgtcgccatg ggcctgcgcg agcatctgca gcacggcgac caatccgtaa
     1561 ggatcgtagc cgctgcgcgc ggcgatgacg atcgccatgc ggtcggcctc gtattccagg
     1621 gcccgatcga ggggccgcag gatgaagccg ctcttcacca ggtccgcgcc catgtcgacg
     1681 agcttctgct tggcggcctg cgcgagcgcg ccgccgccgc tgcgcccgac gcggtcgacc
     1741 gcgac
//
LOCUS       DASVII010000290         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_589200,
            whole genome shotgun sequence.
ACCESSION   DASVII010000290 DASVII010000000
VERSION     DASVII010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589200"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF309_03795"
     CDS             <1..222
                     /locus_tag="VF309_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HEX7465740.1"
                     /translation="VDDDALPAGSEAYDELPDIAARLDLERAMATLSDGERAAISACY
                     YADLSHEEAAVALGMPVGTVKTHVLRAKG"
     gene            249..803
                     /locus_tag="VF309_03800"
     CDS             249..803
                     /locus_tag="VF309_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7465741.1"
                     /translation="MAPAAASTDAELILAILEREDRKAFAELVTRHQSKVRTVLRRLT
                     RGDVALADDLAQETFVLAWKNLARFRFEARFSTWIYRIAFNAWQSEARKKREVRLEVD
                     DDALPPGSEAYDELPDIAARLDLERAMATLSDGERAAIGACYYADLSHEEAAVALGLP
                     LGTVKTHVLRAKAKLRARLVSRKG"
     gene            800..1150
                     /locus_tag="VF309_03805"
     CDS             800..1150
                     /locus_tag="VF309_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465742.1"
                     /translation="MTMISNDGDKLDRLLRRDARAAIDDDGFTTRVMNALPLPSPARY
                     AARREWLKPVLVLGSTALGSALAWLFAPDGVNAAQGMVDLVALRGLTPAAIAMLGMTF
                     TLLIAAIVIAVDTD"
     gene            complement(1154..>1730)
                     /locus_tag="VF309_03810"
     CDS             complement(1154..>1730)
                     /locus_tag="VF309_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465743.1"
                     /translation="AVELYRKGCDLEYHQACHNLGVALRDGKGVPKNAQQGIALMETA
                     CRLGDAKACDDRATETREYERYCALWGAQSCYLAAVALTKESGETALVAEKLVILAMG
                     GVRRGNPAALNVATHLFKDDELACNTDRRNADACAFAGLGYLVGWPGPDRFTQSPEAQ
                     KARALRYLRYACDAGAKNMCAKADALQAAAK"
BASE COUNT          241 a          635 c          575 g          279 t
ORIGIN      
        1 gtcgacgacg acgccctgcc cgcgggctcc gaggcgtacg acgagcttcc ggacatcgca
       61 gcgcggctcg atctcgagcg cgccatggcg acgctcagcg acggcgagcg tgccgcgatc
      121 agcgcctgct actacgccga cctttcgcac gaggaggccg ccgtggcgct cggcatgccc
      181 gtcggaaccg tcaagactca cgtgctgcgc gcgaagggct gagacttcct ccttccttgc
      241 gatccccgat ggcccctgcc gcggcgtcca ccgacgccga gctcatcctt gcgatcctcg
      301 agcgcgagga ccgcaaggcg ttcgccgagc tcgtcacgcg ccaccagtcg aaggtgcgca
      361 ccgtgctgcg ccgcctcacc cggggcgacg tcgcgctcgc cgacgacctc gcccaggaaa
      421 ccttcgtgct cgcgtggaag aatctcgcgc gcttccgctt cgaggcgcgc ttctcgacgt
      481 ggatctatcg catcgccttc aacgcgtggc aaagcgaggc gcgcaagaag cgcgaggtgc
      541 ggctcgaggt cgacgacgac gccctgcccc cgggctccga ggcctacgac gagcttccgg
      601 acatcgccgc gcggctcgat ctcgagcgcg cgatggcgac gttgagcgac ggcgagcgcg
      661 ccgcgatcgg cgcctgctac tacgccgatc tttcgcatga ggaggctgcc gtggcgctgg
      721 gcttgcctct cggaaccgtc aagactcacg tgctgcgcgc gaaagcgaaa ctgcgcgccc
      781 ggctcgtctc caggaaagga tgaccatgat ttcgaacgac ggcgacaagc tcgatcgcct
      841 cctgaggcgc gacgcccgcg cggcgatcga cgatgacggc ttcaccacgc gggtgatgaa
      901 cgccctgccc ctgccctcgc ccgcgcgtta cgccgcgcgg cgcgagtggc tcaagcccgt
      961 gctggtgctc ggctcgaccg ccctggggag cgccctcgcg tggctcttcg ctccggatgg
     1021 cgtcaacgcc gcccagggca tggtcgatct cgtggccttg cgcggcctca cgccggccgc
     1081 catcgccatg ctgggcatga cgttcacgct gctcatcgcc gccatcgtca tcgccgtcga
     1141 taccgactga gagctacttc gcggcggcct gcagcgcgtc ggccttggcg cacatgttct
     1201 tcgcgccggc gtcgcacgcg tagcgaaggt agcgcagcgc gcgcgccttc tgcgcttcgg
     1261 gcgattgcgt gaaccggtcc ggccccggcc atcccaccag gtagccgagg cccgcgaacg
     1321 cgcaggcgtc ggcgttccgg cggtcggtgt tgcaggcgag ctcgtcgtcc ttgaaaaggt
     1381 gggtggcgac gttgagcgcg gccgggttcc cgcggcgaac gccccccatg gcgaggatca
     1441 cgagcttctc agcgaccagc gcggtctcgc cgctttcctt cgtgagggcg accgccgcca
     1501 ggtagcacga ctgggcgccc cagagcgcgc agtagcgctc gtactccctg gtttccgtgg
     1561 cgcggtcgtc gcacgccttg gcgtcaccga gccggcaggc tgtctccatg agcgcgatgc
     1621 cctgctgcgc attcttcggc acgcccttgc cgtcgcgcag cgcgacgccc aggttgtggc
     1681 acgcctggtg gtactccagg tcgcaacctt tcctgtagag ctccaccgcc
//
LOCUS       DASVII010000291         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_14299, whole
            genome shotgun sequence.
ACCESSION   DASVII010000291 DASVII010000000
VERSION     DASVII010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14299"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF309_03815"
     CDS             complement(<1..330)
                     /locus_tag="VF309_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7465744.1"
                     /translation="MQVEVKVPQLSESVSEATLVAWRKKAGEPVKRDENLIDVETDKV
                     VLELPAPADGVLVRIVKNDGESVTSGEVIAVIDTEAKAAAVAAAGDGTPAGDWAPAFA
                     GTTASLSA"
     gene            complement(346..>1505)
                     /gene="sucA"
                     /locus_tag="VF309_03820"
     CDS             complement(346..>1505)
                     /gene="sucA"
                     /locus_tag="VF309_03820"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177796.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7465745.1"
                     /translation="LTDIPSSFKLHPSVERLLAARREMGEGKQALDWGMAENLAYASL
                     VEEGHPVRLSGQDSGRGTFAHRHAVLHDQNREKWDSGVYIPLQNIKEGQANFLVIDSL
                     LSEEAVLGFEYGCSTAQPFELVIWEAQFGDFVNGAQVVIDQFIASGEAKWGRMCGLTM
                     YLPHGYEGQGPEHSSARLERFLQLCAEHNIQVCVPSNAAQFFHMIRRQVIRKMRKPLI
                     VMTPKSLLRKKEAASALQDLATGAFQVIIPETEKLTAKKVKRVVFCAGKVYYDLAAER
                     AKRAVEDVAIVRIEQLYPFPHEEFQAQIDLYPSAKSVVWAQEEPGNQGAWHRIQHYLV
                     RHMRPDQQLSYAGRASSASPAVGYMQLHAEQQKELVEAALNGSAASKKEAA"
BASE COUNT          229 a          497 c          498 g          281 t
ORIGIN      
        1 cgcggaaagg gaagcggtcg ttccggcgaa ggcaggggcc caatctccgg ccggggtgcc
       61 atcgcccgcc gcagcgactg ccgctgcctt cgcctctgta tcgatcaccg cgatcacctc
      121 gccgctggtg acgctctcgc cgtcgttctt cacgatcctc acgagcacgc cgtcggcggg
      181 agcgggaagc tcgagcacca ccttgtcggt ctcgacgtcg atcaggttct cgtcgcgctt
      241 cacgggctct cccgccttct tgcgccaggc gacgagcgtg gcttcggaaa ccgattccga
      301 aagctgggga accttgactt caacctgcat ggtcttgtcc tttggttagg cggcttcttt
      361 tttggacgcg gccgatccgt tcagcgccgc ctcgacgagc tccttctgct gctcggcgtg
      421 caactgcatg tagcccaccg cgggcgaggc cgacgacgcc cgccccgcgt acgaaagctg
      481 ctggtcgggc cgcatgtgac gcaccaggta gtgctggatg cggtgccatg cgccctggtt
      541 gcccggctct tcctgcgccc acacgacgct cttcgcgctc ggataaaggt cgatctgcgc
      601 ctggaattcc tcgtgcggga agggatagag ctgctcgatg cgcacgatcg cgacgtcctc
      661 gacggcgcgc ttggcgcgct ccgccgcgag gtcgtagtag accttgcccg cgcagaagac
      721 gacgcgcttc accttcttcg cggtgagctt ctccgtctcg gggatgatca cctggaaggc
      781 gccggtggcg agatcctgca gcgcggaggc ggcctccttc ttgcgcagca ggctcttcgg
      841 cgtcatcacg atgagcggct ttctcatctt gcgaatgacc tggcggcgga tcatgtggaa
      901 gaactgcgcc gcgttcgaag gcacgcacac ctggatgttg tgctcggcgc acagctgcag
      961 gaagcgctcg aggcgcgccg acgagtgctc gggcccctgg ccctcgtagc catggggaag
     1021 gtacatcgtg aggccgcaca tgcgccccca cttggcctcg ccggaagcga tgaactggtc
     1081 gatgacgact tgcgcgccgt tcacgaagtc gccgaactgc gcttcccaga tgacgagctc
     1141 gaaaggctgc gccgtcgagc agccgtactc gaagccgagc accgcctcct cggagaggag
     1201 cgaatcgatg acgaggaagt tcgcctggcc ttccttgatg ttctgcagcg ggatgtatac
     1261 gccggaatcc cacttctcgc ggttctggtc gtggagcacg gcgtggcggt gcgcgaaggt
     1321 gccgcgtccg ctgtcctggc ccgagaggcg caccgggtgg ccctcctcga cgagggaggc
     1381 gtaggcgagg ttttccgcca tgccccagtc gagcgcctgc ttgccctcgc ccatctcgcg
     1441 ccgcgcggcg agaaggcgct cgaccgacgg atgcagcttg aagctggagg gaatgtcggt
     1501 gagcc
//
LOCUS       DASVII010000292         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_409478,
            whole genome shotgun sequence.
ACCESSION   DASVII010000292 DASVII010000000
VERSION     DASVII010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409478"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF309_03825"
     CDS             <1..533
                     /locus_tag="VF309_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7465746.1"
                     /translation="HIFVGPWSTFSDCDRAKDFLTKSGLSFTSTTLPPDSADKVIMKI
                     GPTEVKGWNEAAVKKALADLKYPTAADKNAINWPMTLLILFIMMIYVTMVYGPIAAFL
                     VELFPTKIRYTSMSLPYHIGNGWFGGMLPLLATAMVAASGDIYYGLWYPIIVALMTVV
                     IGAIFLRDNKDRDIYQ"
     gene            611..1678
                     /gene="lptG"
                     /locus_tag="VF309_03830"
     CDS             611..1678
                     /gene="lptG"
                     /locus_tag="VF309_03830"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX7465747.1"
                     /translation="MLLRYFTREILASSFLVLAALLALFGFFDLIRELDDLGRGTYRI
                     NAMLAYVALTLPSHAYVLLPAAGLIGTLFALARMSEHSEITVMRASGLSLRQLAMHVS
                     VAGLAIALLTAAFGELVTPYTEEAAKGLRLKATSSIVAREFRSGFWVKDDRSFVNIQD
                     VTADTQLLNLRIYEFDPTFRLTAISRAEKGTYAGPNKWDLSNVELTRFEGDRAVLQRI
                     AHATWSSVLTPDILSVLKIVPERMSALNLRAYIDHLRENRQKSTRYEIAFWSKIFSPA
                     AAIVMMILAIPFALTSARTGGVGAKIVLGILIGLSFHFAGRLFAHVGLLNDWPALLSA
                     GGPALISALVATALLVRAERR"
BASE COUNT          272 a          575 c          524 g          324 t
ORIGIN      
        1 tccacatctt cgtcggcccg tggtcgacat tcagcgactg cgaccgggcg aaggacttct
       61 tgaccaaatc ggggttgtcc ttcacgtcga cgacgctgcc gccggattcg gccgacaagg
      121 tgatcatgaa gatcgggccc accgaggtga agggctggaa cgaggcggcc gtgaagaagg
      181 cgctcgccga cctcaagtac ccgaccgcgg cggacaagaa cgccatcaac tggccgatga
      241 cgctcctcat cctcttcatc atgatgatct acgtcaccat ggtgtacggg ccgatcgcgg
      301 cgttcctggt ggagctgttc cccaccaaga tccgctacac gtcgatgtcg ctgccatatc
      361 acatcggcaa cgggtggttc gggggcatgc tgccgctgct cgcgaccgcc atggtcgcgg
      421 cttcgggaga catctactac ggcctctggt acccgatcat cgtggctctc atgacggtgg
      481 tgatcggcgc gatattcctg cgcgacaaca aggaccgcga catctaccag tagaggcgca
      541 agctcacgaa gccccgccgg tttccggcgg ggcttttttt ttgagcgcgc tttatttggc
      601 acaatgcggg atgctcctgc gctatttcac ccgcgagatc ctggcttcaa gcttcctcgt
      661 gctcgcggcg ctgctcgcgc tcttcggatt cttcgacctc atccgcgagc tcgacgatct
      721 cgggcgcggc acctaccgca tcaacgcgat gctcgcgtac gtggcgctca cgctcccgtc
      781 ccacgcttac gtgctgcttc ccgcggcggg gctcatcgga acgctcttcg cgctcgcgcg
      841 catgtcggag cattcggaaa tcacggtgat gcgcgcatcg gggctctcgc tgcgccagct
      901 ggcgatgcac gtttcggtcg cgggcctcgc gatcgcgctc ctcacggccg cattcggcga
      961 gctcgtcacg ccatacaccg aggaggccgc gaagggcctg cgcctgaagg cgacgagctc
     1021 catcgtcgcg cgcgagttcc gctccggctt ctgggtgaag gacgaccgca gcttcgtgaa
     1081 catccaggac gtgaccgcgg atacgcagct tctcaacctg cgcatctacg agttcgaccc
     1141 gacgtttcgc cttacggcga tcagccgcgc cgagaagggc acctatgcgg gtcccaacaa
     1201 gtgggatctc tcgaacgtcg agctcacccg cttcgagggg gacagggcgg tactgcagag
     1261 gatcgcgcac gcgacctgga gctcggtgct cacgcccgac atcctttcgg tgctgaagat
     1321 cgttcccgag cggatgtcgg cgctcaacct gcgcgcctac atcgaccacc tgcgcgagaa
     1381 ccgccagaag tccacgcgct acgagatcgc gttctggagc aagatcttca gccccgccgc
     1441 ggcgatcgtg atgatgatcc tcgcgattcc gttcgcgctc acctcggcgc gcaccggcgg
     1501 cgtgggagcg aagatcgtgc tcggtattct catcgggctg tcgttccact tcgccggccg
     1561 gctcttcgcg cacgtgggcc tgctcaacga ctggcccgcg ctcctgtccg cgggggggcc
     1621 cgcactcatc tccgcgctcg tcgctacggc gctgctggtg cgcgccgaaa ggcgctaggg
     1681 tccggctgcc tcggc
//
LOCUS       DASVII010000293         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_337714,
            whole genome shotgun sequence.
ACCESSION   DASVII010000293 DASVII010000000
VERSION     DASVII010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337714"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF309_03835"
     CDS             <1..425
                     /locus_tag="VF309_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465748.1"
                     /translation="EPVIAAYEGKLAKLRREHAGERRVGALLEIWHRPLTTIAGRHFM
                     NEALGICGARNVFEDLEGVAPVVSMEEVYKRNPEIIVSASSAVSEEEFHAQWREHPTL
                     SAVRTGRLVFVEPDRIQRLTARTPDGIAALCAAVERVR"
     gene            complement(422..1108)
                     /locus_tag="VF309_03840"
     CDS             complement(422..1108)
                     /locus_tag="VF309_03840"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7465749.1"
                     /translation="MRLSPDAIKALAPEYVLGTLRGGARRRLEAMLRDNRALAAEVRL
                     WEDALTPVAEALAPIEPPKRVWSNIESRVASARAAPPASGFWRGFGLVAGGVAAVLFA
                     FFAWISVAPRDEPLFVAVLADTAPRMVVSMHQPDLMRVTVMKPWGGMENRSLELWVLP
                     KQGAPRSLGLVSNVPGDTTLRISPSDPRVQGANALAVSMEPPGGSPTGLPTGAVVCSG
                     MVAPMKSRRA"
     gene            complement(1108..>1721)
                     /locus_tag="VF309_03845"
     CDS             complement(1108..>1721)
                     /locus_tag="VF309_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7465750.1"
                     /translation="LAAGPRGRAEMTLDAPQARLAGLLARAALGDRAAFADLYRATSS
                     KLFAVSLRIVRERPLAEEALQDSFVNIWNHAGDYAQAKSAPTTWMTAIVRNRSLDIVR
                     RTREDPDVDDSLAATLVDESAVPASQAEARAHAHSVRECLEELESEQRQTIALAFFHG
                     LTHSELAAHLRRPLGTVKTHVRRGLAKLRDCLMRNDPDAAERG"
BASE COUNT          251 a          595 c          636 g          239 t
ORIGIN      
        1 tcgagccggt gatcgcggcc tacgagggca agctcgcgaa gcttcgccgc gagcacgcgg
       61 gcgagcgcag ggtgggagcg ctcctcgaga tctggcaccg tcccctcacc accatcgcgg
      121 gccgccactt catgaacgag gcgctcggga tctgcggggc gcgcaacgtc ttcgaggacc
      181 tggagggcgt cgcgcccgtc gtgtcgatgg aggaggtcta caagcgcaat cccgagatca
      241 tcgtgagcgc gagctcggcg gtcagcgagg aagagttcca cgcgcagtgg cgggagcatc
      301 cgacgctgtc ggcggtgcgc acgggaaggc tggtcttcgt ggagcccgat cgcatccagc
      361 gcctcaccgc gcggaccccc gacggaatcg ccgcgctctg cgcggccgtc gagcgcgtgc
      421 gctaggcgcg ccgcgacttc atcggcgcga ccattcccga gcacaccacg gcgcccgtgg
      481 gcaggccggt gggcgatcct ccggggggct ccatgctcac cgcgagcgca ttggcgccct
      541 ggacgcgggg atcgctcggg ctgattcgca gcgtcgtgtc gccgggcacg ttggagacga
      601 ggccgaggga ccggggcgcg ccttgcttcg gaaggaccca cagctcgagg ctgcgattct
      661 ccatgccgcc ccacggcttc atcaccgtca cgcgcatcag gtcgggctgg tgcatcgaga
      721 cgaccatccg cggcgccgtg tcggcgagca ccgcgacgaa cagcggctcg tcgcgcggcg
      781 cgaccgagat ccacgcgaag aacgcgaaaa gcacggccgc gacgcctccc gcgaccaggc
      841 cgaagccgcg ccagaatccg gaggcgggcg gcgccgcgcg cgcggacgcc acccgcgact
      901 cgatgttcga ccagacgcgc ttcggcggct cgatgggcgc gagcgcttcg gcgaccggcg
      961 tgagcgcgtc ctcccagagg cgcacctcgg ccgccaatgc gcggttgtcg cgcagcatcg
     1021 cttcgagccg gcggcgcgcg ccgccgcgca gcgtgccgag cacgtactcg ggagcgagcg
     1081 ccttgatggc atcgggcgag aggcgcatca gccgcgctcg gccgcgtcgg ggtcgttgcg
     1141 catgaggcaa tcgcgcagct tcgcgagccc gcggcggacg tgggtcttca ccgtgccgag
     1201 ggggcgacgc aggtgcgcgg cgagctccga atgggtgaga ccgtggaaga aggcgagcgc
     1261 gatcgtctgg cgctgctccg actcgagctc ctcgaggcat tcgcgcaccg agtgcgcatg
     1321 ggcgcgcgct tctgcctggg acgccgggac cgcggattcg tccacgaggg tcgccgccag
     1381 ggagtcgtcg acatccgggt cctcgcgcgt gcgccgcacg atgtcgaggc tgcggttgcg
     1441 cacgatcgcc gtcatccacg tcgtgggcgc gctcttcgcc tgcgcgtagt ctccggcgtg
     1501 gttccagatg ttgacgaaac tgtcctgcaa cgcttcctcc gcgagcggcc tctcacggac
     1561 tatacgaagc gagaccgcaa acagtttcga gctggtggcg cgatagaggt cggcgaacgc
     1621 ggcgcggtcg ccgagcgcgg ccctcgcgag gagcccggcg aggcgtgctt gcggggcatc
     1681 gagcgtcatt tcggctcgcc ctcgcggccc ggctgccaga t
//
LOCUS       DASVII010000294         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_85636, whole
            genome shotgun sequence.
ACCESSION   DASVII010000294 DASVII010000000
VERSION     DASVII010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85636"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..547)
                     /locus_tag="VF309_03850"
     CDS             complement(98..547)
                     /locus_tag="VF309_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7465751.1"
                     /translation="MPRPRSKVFPTPDDAETAFYDAFERADLAAMMAVWAATDEIVCI
                     HPQGPRLTGFEAVRESWAQLFSGGSQLRVRTVEARTYEGQALAVHNVIEMIAPPGTEG
                     SVTSIFATNVYELTEGGWRMVIHHAAAAPSEANPRREEPASSSHTLH"
     gene            630..1103
                     /locus_tag="VF309_03855"
     CDS             630..1103
                     /locus_tag="VF309_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7465752.1"
                     /translation="MAKFFVNGDPVDFRAAPDTPLLWALRDQLGLTGTKFGCGMAQCG
                     ACTVHVDQQPTRACVTPVSAIEGKRVVTIEGIYNTSQGATAKAVRAAWVDKDVVQCGY
                     CQSGQVMSAVALLQRNKSPSDADIDAAMSGNICRCGTYQRIREAIKSAAASLKKA"
     gene            1111..>1801
                     /locus_tag="VF309_03860"
     CDS             1111..>1801
                     /locus_tag="VF309_03860"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7465753.1"
                     /translation="MTMKSTSRRRFLQSSATLAGGLVISFWLPVGMGRKAFAQAPQPA
                     KVPPNAFLRIAKDGSVTVVVKHLEFGQGVTTSLPMILCEELDCDWTKVRYELAPAAPE
                     YAHTMFGMQMTGGSSSVSNSFEQMRTVGAQARAMLMQAAANQWKVKVSDVHTEKGFVV
                     GPGGKKLSYGQLADAAGKLPVPEKVALKDPKSFTLIGQPTRRIDSAEKVEGKATFGLD
                     VKRKDLHTAVVA"
BASE COUNT          309 a          594 c          615 g          283 t
ORIGIN      
        1 gtcactgtac ccagtatccg agaagccaca ccttgatggg gtacgttcat ctgcgctaca
       61 tcggcgttca tctgcgtttc cgcactaacg cgacagctca gtgaagggtg tgcgaggagg
      121 aagcgggctc ttcgcggcgg ggattggcct cggatggcgc ggccgcggcg tggtggatca
      181 ccatccgcca gccgccctcg gtgagctcgt agacgttggt ggcgaagatg gaggtcaccg
      241 agccctcggt gccgggcggc gcgatcatct cgatcacgtt gtgcaccgcg agcgcctggc
      301 cttcgtaggt gcgcgcctcg acggtgcgca cgcgcagctg cgatccgccc gagaatagct
      361 gcgcccagct ctcgcgcacc gcctcgaacc cggtgaggcg cgggccctgc ggatggatgc
      421 agacgatctc gtcggtggcg gcccacaccg ccatcatcgc ggccaggtcg gcgcgctcga
      481 aggcgtcgta gaacgcggtc tcggcgtcgt cgggagtggg gaaaaccttg ctgcggggac
      541 ggggcatgag ggcgtggcga ggtgcggcgt gcgaatgata ccaaaggcat aatgatgggt
      601 cccgcttccc catattccgc gagacttcca tggccaaatt cttcgtgaac ggcgacccgg
      661 tcgacttccg cgccgccccc gatacccccc tcctctgggc gctgcgcgac cagctcggcc
      721 tcaccggcac caagttcggc tgcggcatgg cgcaatgcgg ggcgtgcacg gtgcacgtcg
      781 accagcagcc gacccgcgcc tgcgtgacgc ccgtgtccgc catcgagggc aagcgcgtgg
      841 tcaccatcga gggcatctac aacacctcgc agggcgccac cgcgaaggcg gtgcgggcgg
      901 cgtgggtgga caaggacgtc gtgcagtgcg gctactgcca gtcgggacag gtgatgagcg
      961 ccgtggccct gctgcagagg aacaagtcgc cgtcggacgc ggacatcgac gccgcgatga
     1021 gcggcaacat ctgccgctgc ggcacctacc agcgcattcg cgaggcgatc aagagcgccg
     1081 ccgcatcgct gaagaaagcg tgaggaccac atgaccatga aatcgacttc gcgccggcgt
     1141 ttcctgcaat cttccgccac cctcgcgggc ggcctcgtca tctcgttctg gctgccggtg
     1201 ggcatggggc gcaaagcctt cgcgcaggcg ccgcagcccg ccaaggtccc gcccaacgcc
     1261 ttcctgcgca tcgccaagga cggctcggtc acggtagtgg tgaagcatct cgagttcggc
     1321 cagggcgtca ccacgtcgct gccgatgatc ctttgcgagg agctcgattg cgactggacc
     1381 aaggtgcgct acgagctcgc gcccgccgcg cccgagtacg cgcacacgat gttcggcatg
     1441 cagatgaccg gtggatcgtc gtcggtttcc aactccttcg agcagatgcg caccgtcggc
     1501 gcgcaggcgc gcgcgatgct gatgcaggcg gccgccaacc agtggaaggt gaaggtctcc
     1561 gacgtacaca ccgagaaggg ctttgtcgtg ggaccgggcg gcaagaagct ttcctatggc
     1621 cagctcgcgg acgccgccgg caaattgccg gtgccggaga aagtcgcgct caaggacccg
     1681 aagagcttca cgctcatcgg ccagcccacg cggcgcatcg actccgccga gaaggtcgag
     1741 ggcaaggcga ccttcggcct cgacgtgaag aggaaggacc tccataccgc ggtggtcgcg
     1801 c
//
LOCUS       DASVII010000295         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_265584,
            whole genome shotgun sequence.
ACCESSION   DASVII010000295 DASVII010000000
VERSION     DASVII010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265584"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1729
                     /gene="aceE"
                     /locus_tag="VF309_03865"
     CDS             <1..>1729
                     /gene="aceE"
                     /locus_tag="VF309_03865"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX7465754.1"
                     /translation="RDKDGFLLKRMEDAVDGEYQKFKSRDGAYVREHFFGKYPETRAM
                     VANMSDADVWRLNRGGHDPHKVYAAYAAAVKHKGQPTVILAKTVKGYGMGAVGEGKNV
                     AHQQKKMPIEALMQFRDRFNIPIADDKLSELPYYKPAEDSPEMKYLKELRAQRGGSLP
                     QRRRKSVKLDVPKLEAFKPLLDATAEGREISTTMAFVRMLTILVKDKGVGKNVVPIVP
                     DESRTFGMEGLFRQLGIYSQVGQLYEPEDSDQLMFYKEDKSGQILQEGINEAGAMCSW
                     MAAATSYSNNNVPMIPFFIFYSMFGMQRVGDLVWASGDLRARGFLMGGTAGRTTLNGE
                     GLQHEDGHSHIISALVPNCISYDPTFAYEVAVIVHDGLRRMVENQEDVFYYITLMNEN
                     YPHPGLPEGARDGILKGMYRISDAGAATGARVQLMGSGTILREVMFAADLLKKDWGVS
                     ADVWSCPSFSELRRDGVAAARWNLLHPAEKPRVSYVEKCLAGTKGPVIASTDYVRTFA
                     DQIREFVPRRYKVLGTDGFGRSDSRENLRRFFEVNRHYVAIAALKALAEDGEIAPAKV
                     VEAIRKYGID"
BASE COUNT          331 a          561 c          574 g          263 t
ORIGIN      
        1 cgcgacaagg acgggttcct cctcaagcgc atggaagacg cggtcgacgg cgagtaccag
       61 aaattcaaga gccgcgacgg cgcctacgtg cgcgagcatt tcttcggcaa gtatccggag
      121 acgagggcga tggtcgccaa catgtccgac gccgacgtgt ggcgcctgaa tcgcggcggc
      181 cacgacccgc acaaggtcta tgccgcctat gcggccgccg tcaagcacaa gggccagccc
      241 acggtgatcc tcgccaagac cgtgaagggc tacggcatgg gcgcggtcgg cgaaggcaag
      301 aacgtggcgc accagcagaa gaagatgccc atcgaggcgc tgatgcagtt ccgcgaccgc
      361 ttcaacattc cgatcgccga cgacaagctc tccgagctcc cgtactacaa gccggcggaa
      421 gactcgccgg agatgaaata cctgaaggag cttcgcgcgc agcgcggcgg gtcgctgccg
      481 cagcgccggc gcaagagcgt caagctcgac gtgccgaagc tcgaggcgtt caagccgctc
      541 ctcgacgcga ccgccgaggg ccgtgagatc tccacgacga tggccttcgt gcgcatgctg
      601 accatcctcg tgaaggacaa gggcgtcggc aagaacgtcg tgcccatcgt ccccgacgaa
      661 tcgcgcacct tcggcatgga gggcctcttc cgccagctcg ggatctattc gcaggtgggg
      721 cagctctacg agcccgagga cagcgaccag ctcatgttct acaaggagga caagagcggg
      781 cagatcctgc aggaaggcat caacgaggcg ggcgcgatgt gctcgtggat ggcggcggcg
      841 acgtcgtact cgaacaacaa cgtgccgatg atcccgttct tcatcttcta ctcgatgttc
      901 ggcatgcagc gcgtgggcga cctcgtttgg gcctcggggg acctgcgcgc gcgcggcttc
      961 ctcatgggcg gcaccgcggg acgcacgacc ctgaacggcg agggcctgca gcacgaggac
     1021 gggcacagcc acatcatctc ggcgctcgtt ccgaactgca tctcctacga tcccacgttc
     1081 gcctacgaag tcgcggtgat cgtccacgac gggctgcgcc gcatggtgga aaaccaggag
     1141 gacgttttct actacatcac gctgatgaac gagaactacc cccaccccgg gctgcccgag
     1201 ggcgcgcgcg acggaatcct gaagggcatg taccggatct ccgatgcggg cgccgctacc
     1261 ggggcgcgcg tgcaattgat gggaagcggc acgatccttc gcgaggtgat gttcgccgcc
     1321 gacctgctga agaaggattg gggcgtgtcg gccgacgtgt ggagctgccc gagcttcagc
     1381 gagcttcgcc gcgatggcgt cgccgcggcg cgatggaacc tgctgcatcc cgccgagaag
     1441 ccgcgtgtct cgtacgtcga gaagtgcctc gccggcacca agggcccggt gatcgcctcg
     1501 accgactacg tgcgcacctt cgccgaccag atccgcgagt tcgtgccgcg ccgctacaag
     1561 gtgctcggca ccgacgggtt cgggcgcagc gattcgcgcg agaacctgcg ccgcttcttc
     1621 gaggtgaatc gccactacgt cgccatcgcc gcgctgaagg cgctcgccga ggatggcgag
     1681 atcgcgcccg cgaaggtggt ggaggccatc aggaaatacg gcatcgaca
//
LOCUS       DASVII010000296         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_85688, whole
            genome shotgun sequence.
ACCESSION   DASVII010000296 DASVII010000000
VERSION     DASVII010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85688"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VF309_03870"
     CDS             complement(<1..611)
                     /locus_tag="VF309_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7465755.1"
                     /translation="MQFIVEGYPAYAYTGGRAFDPGLPAIVFIHGAAFDHSVWQWQSR
                     YFAHHGFCVLAVDLPGHGRSPGTPRATIEDGSRWIASFIDSAALERPALVGHSMGSLI
                     ALETALRHGERISKLALVGTATPMPVGDAFLAAARDDSPAALDMEAVWGHSRQSQLAQ
                     SPVPGMNLLGASRMLNGRSRPGALAAALDACNRYRVSKEALNAL"
     gene            complement(618..1916)
                     /locus_tag="VF309_03875"
     CDS             complement(618..1916)
                     /locus_tag="VF309_03875"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006004.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX7465756.1"
                     /translation="MTHSAPKTPHPDTLAVHAGQSPDPATGSRAVPIYQTASFVFPDT
                     DTAAALFNMETPGHVYSRLSNPTVAVFEERMACLEDGVGAIATASGQAALHLAIVTLM
                     GQGGHIVASSSLYGGSHNLLAYTLPRFGITTTFVASRDLAAWRKAIKPETRLLFGESV
                     GNPSVDVLDIPAVAGLAKEHRIALLVDATVSTPSLGRPFHQGADLVMHSATKFLSGHG
                     VVIGGVLVDGGRFDWLASGKFPTLTEPYEGFHGVVFAEESTTSAFLLRARREGLRDFG
                     ACMAPMTAFQILQGLETLPLRMARHISNTERVVEFLASQKEVEKVHHPHLATHPDHAT
                     AKRLYPDGCSAILAFELKGGRDAGRKFIEALTLFSHLANIGDAKSLVIHPATTTHFRM
                     DDGALAAAGITPGTVRLSIGLEHPGDLLEDLARALRASQK"
     gene            2021..2920
                     /gene="pbpG"
                     /locus_tag="VF309_03880"
     CDS             2021..2920
                     /gene="pbpG"
                     /locus_tag="VF309_03880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX7465757.1"
                     /translation="MIRLVATLLLAALPLYAGAAAGLNSKPRAAKVATAEFTKDGEPN
                     VQSSGVLVFDPTTGQILFAKNAEQVAPIASITKLMTAMVVLDAKLAMDEPIQITTDDI
                     DLVKNTRSRLPIGTHFRRDDLMRLALMASDNRAASALGRNYPGGVAAIVVAMNDKAKA
                     LGLTQTHYVDASGLSPANVSSPADLGKLVAAAATYPVITEYTTTGAVNVTLPDSKRKM
                     SFVNTNALVRNSDWKIGLSKTGYINEAGKCLVMHAMIANQPIVIVLLDSWGRLTRIAD
                     ANRIRKWLEKNPSKLVGATLHPG"
BASE COUNT          522 a         1035 c         1021 g          482 t
ORIGIN      
        1 agcgcgttga gcgcttcctt cgatacgcga tagcgattgc acgcgtcgag agccgccgca
       61 agcgcgccgg gcctcgatcg cccgttgagc attcggctcg cgcccagcag gttcatgccg
      121 ggcacggggc tctgcgcgag ctgcgactgc cgcgaatgtc cccataccgc ttccatgtcg
      181 agcgccgcgg gagaatcgtc gcgcgccgcc gcgaggaacg cgtccccgac gggcatcggc
      241 gtcgcggtgc ccacgagcgc gagcttcgag atccgttcgc catgccggag cgccgtctcg
      301 agcgcgatga gcgatcccat gctgtggccg acgagcgccg ggcgctcgag ggccgccgaa
      361 tcgatgaacg acgcgatcca gcgcgacccg tcctcgatcg tggcgcgcgg cgtgcccggg
      421 ctgcggccat ggccgggaag atcgaccgcc agcacgcaga atccgtggtg cgcgaaatat
      481 cgcgactgcc attgccagac gctgtggtcg aacgccgcgc cgtggataaa cacgatcgcc
      541 ggaagcccgg gatcgaacgc gcgtccgccg gtgtacgcat aggccggata gccctcgacg
      601 atgaattgca tcgcggccta cttctgcgag gcgcgcaggg cccgcgcgag atcttcgagg
      661 agatcaccgg gatgctcgag gccgatcgac aggcgcaccg tgccgggcgt gattcccgcg
      721 gcggcgagcg ccccatcgtc catccggaag tgcgtcgtgg tcgcggggtg gatcaccagg
      781 gacttcgcgt ccccgatgtt cgccaggtgc gagaagagcg tgagcgcctc gatgaacttg
      841 cgccccgcgt cgcggccgcc cttcagctcg aaggcgagga tcgcgctgca tccgtcggga
      901 tagaggcgct tcgccgtcgc gtgatcggga tgcgtcgcga gatggggatg gtggaccttc
      961 tcgacttcct tctgcgacgc caggaactcg accacgcgct cggtgttgga gatgtggcgt
     1021 gccatgcgca gcggcagcgt ctcgagtccc tgcaggatct ggaacgcggt catcggcgcc
     1081 atgcaggcgc cgaagtcgcg caggccctcg cggcgcgcgc gcagcaggaa tgccgaggtg
     1141 gtcgattcct ccgcgaaaac cacgccgtgg aagccctcgt agggctcggt gagcgtcgga
     1201 aatttccccg acgcgagcca atcgaagcgg ccgccgtcga cgagcacgcc gccgatcacc
     1261 acgccatggc ccgagaggaa cttggtcgcc gaatgcatga cgagatccgc gccctggtgg
     1321 aacggcctgc cgagcgaggg cgtgctcacc gtcgcgtcca ccagcaacgc aatgcggtgc
     1381 tctttcgcga ggcccgcgac ggcggggatg tcgagcacgt ccaccgaagg attgccgacg
     1441 ctctcgccga acaggagccg cgtctcgggc ttgatggcct tgcgccacgc cgcgaggtcg
     1501 cgcgacgcga cgaacgtcgt cgtgatgccg aagcgaggca gcgtgtacgc gaggagattg
     1561 tgcgagccgc cgtacaggct cgacgaggcg acgatatggc cgccctggcc catcagcgtg
     1621 acgatggcca ggtgcagcgc ggcctgcccg ctcgcggtgg ctatcgcgcc gacgccatcc
     1681 tccaggcagg ccatgcgttc ctcgaacacg gccacggtgg gattcgaaag gcgggagtac
     1741 acgtggcccg gcgtttccat gttgaaaagc gccgccgcgg tgtcggtgtc cgggaagacg
     1801 aaagacgccg tctggtagat cggcacggcc cgggatccgg tggccggatc gggcgactgg
     1861 ccggcgtgga ccgccaaagt gtcgggatgg ggggttttgg gggccgaatg ggtcatccaa
     1921 actgatccaa atcaattgtt taggatgtaa ttttaagatc ttgtcacgaa taggggttgt
     1981 ttgctatggt gtcgcctgcc ctcctgtcac ccggaatccc gtgattcgac tcgtcgccac
     2041 ccttctgctg gccgcccttc ccttgtacgc gggggccgcc gccggcttga attccaagcc
     2101 gcgcgccgcc aaggtcgcga ccgccgaatt caccaaggac ggcgagccga acgtgcaatc
     2161 gtcgggcgtg ctcgtgttcg atccgaccac gggccagatc ctcttcgcga agaacgccga
     2221 gcaggtcgcg ccgatcgcat ccatcaccaa gctcatgacg gcgatggtgg tcctcgacgc
     2281 gaagctcgcg atggacgagc cgatccagat caccacggac gacatcgatc tcgtgaagaa
     2341 cacgcgctcg cgccttccga tcggcacgca cttccgccgc gacgacctca tgcgcctcgc
     2401 gctgatggcg agcgacaacc gcgcggcctc ggccctcggg cgcaactatc ccggcggcgt
     2461 cgcggcgatc gtcgtcgcga tgaacgacaa ggcgaaggcg ctcggcctca cccagactca
     2521 ctacgtcgac gcctcggggc tgtcgcccgc caacgtgtcg agcccggcgg acctgggcaa
     2581 gctcgtcgcc gccgcggcga cctatcccgt catcaccgag tacacgacca cgggcgcagt
     2641 caacgtgacg ctgcccgact cgaagcgcaa gatgtccttc gtgaacacca acgcgctggt
     2701 gcgcaactcc gattggaaga tcgggttgtc gaagaccggc tacatcaacg aggccggcaa
     2761 gtgcctggtg atgcacgcga tgatcgcgaa ccagccgatc gtcatcgtgt tgctcgattc
     2821 gtggggccgc ctcacgcgca tcgccgatgc caaccgcatc cgcaagtggc tcgagaagaa
     2881 cccgagcaag ctcgtcggcg ccacgctgca cccgggctga tcgcgcctgc cgcgatcacg
     2941 tcatccccgc gcaggcgggg acccaattag ataccggcgc gccataacag ggcgcgccgt
     3001 ttttctttgc accgttgcgg agcatcgccc ctcccgcgcc tcctgcccca cgcctgcatt
//
LOCUS       DASVII010000297         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_193372,
            whole genome shotgun sequence.
ACCESSION   DASVII010000297 DASVII010000000
VERSION     DASVII010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193372"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VF309_03885"
     CDS             <1..770
                     /locus_tag="VF309_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX7465758.1"
                     /translation="QWQWLEDAKTRAITTNDADQYNSLHERLIEGWKNIGKGGAVHLA
                     GDLGSAEDAGTLAYLEDTARQAGLTTTMLAMDNIGRKPNGAFVDLADKPIDLAFKLYP
                     WEWMFREQFGASLAGSPTRWLEPPWKAILSNKGILPLLWAMFPRHPNLLPAYFEDDEK
                     AAELGNSFVRKPLYSREGANVAIVVGGEAIDQDSGPYGEEGFVRQAVATLPRFDGNYA
                     AIGSWIAAGQPCGLSVREDMSPITKNTSRFLPHAIVG"
     gene            829..>1597
                     /locus_tag="VF309_03890"
     CDS             829..>1597
                     /locus_tag="VF309_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7465759.1"
                     /translation="MNFQPKFDSAALLADPVPPLPPRRDPKQTNSLILRLSVVGFIVA
                     LASRATDPIIPPIAHDIQVDPNAVALLTTAFALPFALVQPVLGPVGDMVGKVRVMIAC
                     LAVTILAMLASGVATNFTTLLVARIVAGAAAGGIFPVGIAVIGDLVPVKERQVAIARW
                     LTAGITGNLLGSSLAGVIGDLTGWRGVFFVITGLGVVALGVAIVSLREAARAKPARLS
                     LSGFSAGYASVFANPRAKVCFGAVFVEGIVIFGLFPFI"
BASE COUNT          250 a          550 c          515 g          282 t
ORIGIN      
        1 tccagtggca atggctggag gatgccaaaa cgcgggcgat cacgacgaac gatgccgacc
       61 agtacaactc gctgcacgag cggctgatcg agggctggaa gaacattgga aaaggcggcg
      121 cggttcacct cgcgggcgat ctcggcagcg cggaggatgc aggcacgctc gcctatctgg
      181 aagacaccgc gcgccaagcc ggcctcacca ccacgatgct ggcgatggac aatatcggcc
      241 gcaagccgaa cggcgccttc gtcgacctcg ccgacaagcc gatcgacctc gcgttcaagc
      301 tttacccctg ggagtggatg ttccgcgagc agttcggcgc ctcgctcgca ggctcgccga
      361 cgcgctggct cgagccgccg tggaaggcga tcctctcgaa caagggcatc ctgccgctgt
      421 tgtgggcgat gtttccgcgc catccgaatt tgctgcccgc ctatttcgag gacgacgaaa
      481 aggccgccga gctcggcaat tccttcgtgc gcaagccgct ctattcgcgc gagggcgcga
      541 acgtggcgat cgtggtcggc ggcgaggcga tcgatcagga ctccgggccc tatggcgagg
      601 agggattcgt ccgccaggcg gtcgcgacgc tgccgcgctt cgacgggaac tacgccgcga
      661 tcggctcctg gatcgccgca gggcagccat gcggcctgtc agtgcgcgag gatatgagcc
      721 caatcaccaa gaatacctcg cgattcctgc cccacgcgat tgtcgggtga gccattcccg
      781 cactgcacaa caaatcgtcg gaaaatcatg tatgggtctc gccaccccat gaatttccag
      841 cccaaattcg attccgccgc gttgctcgct gacccggttc ccccgctccc tcccaggcgc
      901 gacccgaagc agaccaacag cctgatcctg cgcctgagcg tggtcggctt catcgtggcg
      961 ctcgcctcgc gcgcgaccga cccgatcatt ccgccgatcg cccacgacat tcaggtcgac
     1021 ccgaacgcgg tcgcgctgct caccaccgcg ttcgcgctgc cattcgcact ggtgcagccg
     1081 gtgctcgggc ccgtgggcga catggtcggc aaggtgcgcg tcatgatcgc gtgccttgcg
     1141 gtcacgattc tcgccatgct ggcgagcggc gtagcgacca acttcaccac cctgctggtg
     1201 gcgcgcatcg tcgcgggcgc ggcggccggc ggcatcttcc cggtcggcat cgcggtcatc
     1261 ggagacctcg tgcccgtgaa ggagcgccag gtcgcgatcg cgcgctggct cacggcgggc
     1321 atcaccggca atctgctcgg ctcctcgctc gcgggcgtga tcggcgatct caccggctgg
     1381 cgcggcgtgt tcttcgtcat caccgggctc ggtgtcgtcg cactcggcgt cgcgatcgtt
     1441 tcgctgcgcg aggccgcgcg cgccaagccc gcgcggctca gcctctcggg ctttagcgcc
     1501 ggttacgctt ccgtgttcgc gaacccgcgc gcgaaagtgt gcttcggcgc ggtgttcgtc
     1561 gagggcatcg tgatcttcgg gctgttcccg ttcatcg
//
LOCUS       DASVII010000298         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_481339,
            whole genome shotgun sequence.
ACCESSION   DASVII010000298 DASVII010000000
VERSION     DASVII010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481339"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VF309_03895"
     CDS             <1..1109
                     /locus_tag="VF309_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465760.1"
                     /translation="NGVKIAFEECETGYATDRGVECYERLKSKSPVAVNPLPTGITYA
                     LTDKVAADKVVLFTTGYGRSESVDGGVFKYNFPLMGTYWDAADILVQHVKKKGSLKGK
                     KIALVYHDSPYGKEPIPVLEELAKREGFELLKLPVTHPGVEQKATWLQIRQQRPDYVF
                     LWGWGVMNSTAIKEAIAVSYPRLQMYGVWWAAAEPDVLPAEMAAKGYNGLALEHSSEN
                     NAASHKDMVKYLYDKGKGTAAKKEEVGSVLYNRGMLSAMLTVESIRVAQGKFGKRVMT
                     GEEVQWGAENLNIDAARIKALGVDGILQPIKTACNNHEGASKARIHTWDGSKWSYTSD
                     WYQSDAKLLRPMIDAAAKKYAAEKKIETRDCGKV"
     gene            1125..1925
                     /locus_tag="VF309_03900"
     CDS             1125..1925
                     /locus_tag="VF309_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465761.1"
                     /translation="MLEVNGIEVIYNHVILVLKGVSLAVPEGGIVALLGANGAGKTTT
                     LKAISNLLRAERGDVTKGSVEFAGARVDKLNPSELVKRGVIQVMEGRHCFAHLTIEEN
                     LMTGAYTRKDGRAGIAATLDKVYRYFPRLKERRTSQAAYTSGGEQQMCAIGRALMANP
                     KMVLLDEPSMGLAPQIVEEVFEIVKDLNQKEGVTFLLAEQNTNMALRYADYGYILESG
                     RVVMDGAAAMLRENEDVREFYLGTGAGSGERKSYRDVKSYKRRKRWLS"
     gene            1955..>2160
                     /locus_tag="VF309_03905"
     CDS             1955..>2160
                     /locus_tag="VF309_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465762.1"
                     /translation="MARADAEALKTLVPLLRQLREIKGVKEKTPGIFYGRNDAFIHFH
                     DEAGVLFADLKKAGGAGFDRYPLAT"
BASE COUNT          444 a          671 c          735 g          310 t
ORIGIN      
        1 tgaacggcgt gaagatcgcg ttcgaggaat gcgagaccgg ctacgccacc gaccgcggcg
       61 tcgagtgcta cgagcgcctc aagagcaaga gtccggtcgc cgtgaacccg ctccccaccg
      121 gcatcaccta cgctcttacc gacaaggtgg ccgccgacaa ggtcgtcctc ttcaccacgg
      181 gctacggacg ctctgagagc gtcgacggcg gcgtcttcaa gtacaacttc ccgttgatgg
      241 gcacctactg ggacgcggcc gacatcctcg tgcaacacgt gaagaagaag ggcagcctca
      301 agggcaagaa gatcgcgctg gtctatcacg attcgccgta cgggaaggaa ccgatcccgg
      361 tcctcgagga gctggcgaag cgggaaggct tcgagcttct caagctgccg gtcacgcacc
      421 cgggcgtcga gcagaaggcg acgtggctgc agatccgcca gcagcgtccc gactacgtct
      481 tcctgtgggg gtggggcgtg atgaattcca ccgccatcaa ggaggcgatc gcggtttcct
      541 atccgcgcct gcagatgtac ggcgtgtggt gggccgccgc cgagcccgac gtgctgcccg
      601 ccgagatggc ggccaagggc tacaacggcc tcgcgctgga gcactcctcc gagaacaacg
      661 ccgcgagcca caaggacatg gtcaagtacc tctacgacaa gggcaagggg accgcggcca
      721 agaaggaaga ggtcggctcg gtgctctaca accgcggcat gctgtccgcg atgctcaccg
      781 tggaatcgat ccgcgtggcg cagggcaagt tcggcaagcg cgtgatgacc ggcgaagagg
      841 tccagtgggg cgcggagaac ctgaacatcg acgcggcgcg catcaaggcg ctcggcgtgg
      901 acggcatcct gcagcccatc aagaccgcgt gcaacaacca cgagggcgcg agcaaggccc
      961 gcatccatac gtgggacggc tccaagtgga gctacacctc cgactggtac cagtccgacg
     1021 cgaagctgct gcgcccgatg atcgacgcgg cggcaaagaa gtacgcggcc gagaagaaga
     1081 tcgagacgcg cgactgcggg aaggtctagc gggcggtgaa accgctgctg gaggtgaacg
     1141 gcatcgaggt gatctacaac cacgtgatcc tcgtgctgaa aggcgtttcg ctcgcggttc
     1201 ccgagggggg catcgtcgcc ctcctcgggg ccaacggcgc cggcaagacc acgacgctga
     1261 aagcgatatc gaacctgctg cgcgcggagc gcggcgacgt gaccaagggc tcggtcgaat
     1321 tcgccggcgc gcgcgtggac aagctgaacc cctcggagct ggtgaagcgt ggcgtgatcc
     1381 aggtgatgga aggccggcac tgcttcgcgc acctgacgat cgaggaaaac ctgatgaccg
     1441 gggcctacac gcgcaaggac ggcagggcgg ggatcgcggc aacgctcgac aaggtgtatc
     1501 gctactttcc gcgtctgaag gaacggcgca cgtcgcaggc ggcctacacc tcgggcggcg
     1561 agcagcagat gtgcgcgatc ggccgggcct tgatggccaa cccgaagatg gtgctgctcg
     1621 acgagccgtc gatgggcctg gcgccgcaaa tcgtcgaaga ggtgttcgag atcgtgaagg
     1681 acctgaacca gaaggaaggc gtgaccttcc tgctcgcaga gcagaacacc aacatggcgc
     1741 tgcgctatgc cgactacggc tacatcctgg aaagcggccg cgtcgtcatg gatggcgccg
     1801 cggcgatgct gcgcgagaac gaggacgttc gcgagttcta cctcggtacc ggggccggct
     1861 cgggcgaacg caagagctac cgcgacgtga agagctacaa gcgccgcaag cggtggctct
     1921 cgtagggcct gttaacaggc cctaaggaga cgacatggca cgcgccgacg ccgaagccct
     1981 gaagaccctc gtgccgctgc tgcgccagct gcgcgagatc aaaggcgtga aggagaagac
     2041 gcccggcatc ttctacggcc gcaacgacgc tttcatccac ttccacgacg aggctggcgt
     2101 gctgttcgcc gacttgaaga aggccggcgg cgccggcttc gaccgctatc cgctggcgac
//
LOCUS       DASVII010000299         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_517299,
            whole genome shotgun sequence.
ACCESSION   DASVII010000299 DASVII010000000
VERSION     DASVII010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517299"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1426)
                     /locus_tag="VF309_03910"
     CDS             complement(83..1426)
                     /locus_tag="VF309_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465763.1"
                     /translation="MNHTMKALLVSCGALASALGVGAGTTPAHAEGKTITLCWAAWDP
                     ANALVELSKDFTTKTGIGMKFEFVPWTNYADRFLNELNSKGSLCDLIIGDSQWIGGAA
                     ENGQYVKLNDFFKKEGISMDDYMPATVVGYSEWPKNTPNYWALPAMGDAVGWTYRKDW
                     FARPDVREAFKAKYNRDLAVPKTLDELHDIAQFFQGREIDGKKVYGASIYTERGSEGI
                     TMGVSNYLYDYGFKYQDPNKPYAMEGFVNSAGAVKGLEAYKELYKCCTPPGASNSYMS
                     EGLDAFKSGQVALQMNFFAFFPGLYKDPNVGGDKIGFFSNPGATTQATQLGGQGISVV
                     AYSKNQGEALQYIKWFAGGDVQKKWWALGGYSCAKSVLNDPSFPNSAPFAGEFLKSMA
                     MVVDFWAEPSYAQLLQAEQKRVHDYVVADKGTAKEALDGLVKDWQAIFKEEGKKF"
     gene            complement(1711..>1902)
                     /locus_tag="VF309_03915"
     CDS             complement(1711..>1902)
                     /locus_tag="VF309_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465764.1"
                     /translation="SADQVRSASGSVSSAAAKLAEEVSKFFLVLRSGPMERRKADDPN
                     YGGIERRNGETRSRAGRAG"
BASE COUNT          339 a          600 c          575 g          388 t
ORIGIN      
        1 ccgggaccgc cggcgatgcg acgggttccg gtattgtcaa aagaatccgg gagcgcgatg
       61 gcgcgcgctc ccggcgattg tcctagaact tcttgccttc ttccttgaag atggcctgcc
      121 agtccttcac cagaccgtcg agcgcttcct tcgccgtgcc cttgtcggcc acgacgtagt
      181 catgaacgcg cttctgttcg gcctgcagca gctgcgcgta ggacggctca gcccagaagt
      241 cgacgaccat cgccatcgac ttcaggaact cgcccgcgaa cggcgcgctg ttcgggaagc
      301 tcgggtcgtt cagcacggac ttggcgcagg aataaccgcc gagcgcccac cacttcttct
      361 gcacgtcgcc gccggcgaac cacttgatgt attgcagcgc ctcgccctgg ttcttggaat
      421 aggccacgac ggaaatgccc tgaccgccga gctgcgtcgc ctgggtcgtc gcacccggat
      481 tggagaagaa gccgatcttg tcgccaccga cgttcgggtc cttgtagaga ccggggaaga
      541 aggcgaagaa gttcatctgc agcgccacct gccccgactt gaaggcatcg agaccttccg
      601 acatgtagga gttggaggcg ccgggcggcg tgcagcactt gtagagttcc ttgtaggctt
      661 cgagcccctt caccgcgccg gccgagttga cgaaaccctc catcgcatag ggcttgttgg
      721 ggtcctgata cttgaagccg tagtcgtaga gatagttcga cacgcccatt gtgatgcctt
      781 ccgagccgcg ttcggtatag atcgacgcgc catagacctt cttgccgtcg atctcgcggc
      841 cctggaagaa ctgcgcgatg tcgtggagtt cgtcgagcgt cttcggcacg gcgagatcgc
      901 ggttatactt ggccttgaag gcctctcgca cgtcgggacg ggcgaaccag tccttgcgat
      961 aggtccagcc gaccgcgtcg cccatcgccg gcaacgccca gtagttcggc gtgttcttcg
     1021 gccattcgga atagccgacc acggtcgccg gcatgtagtc gtccatgctg atcccttcct
     1081 tcttgaagaa atcattgagc ttgacgtact ggccgttctc ggcggcgccg ccgatccact
     1141 gcgaatcgcc gatgatcaga tcgcacaatg atcccttcga attgagttca ttgaggaagc
     1201 gatcggcata gttggtccag ggcacgaatt cgaacttcat gccgataccg gtcttggtgg
     1261 tgaagtcctt ggataactcg accagcgcgt tggccgggtc ccacgccgcc cagcacagcg
     1321 tgatcgtctt gccctcggcg tgcgcaggcg tggtgcctgc gccgacgccg agcgcggagg
     1381 caagtgctcc gcatgaaacc agaagcgcct tcatcgtgtg gttcattacg cgttccctcc
     1441 cagtggagcc ggccatcgac gtggcgcagc tttgctaccc gctcgaaggc ttcgagcaga
     1501 gcgacacagt ccttgacgag atgaatgact gacctgacgt ttcctcgcgc ccatccgaat
     1561 tcttacgatc cggaggagct cgatttgttt agtggatcat gatttactaa acattgcaag
     1621 cgcgcgctga cggctcgcgt tccggcgagc taagcttggc agagctgggc gtacccgcgg
     1681 ctcagcccag cactatcgcg gtcgcggctc ctagccggct cggcccgcgc gcgagcgcgt
     1741 ttcgccatta cggcgctcga tcccgccgta gttcggatcg tctgccttgc ggcgttccat
     1801 gggaccggaa cgcagcacca gaaagaactt gctgacctct tcggcgagct ttgcggccgc
     1861 gctcgacacc gagccggaag cggaccggac ctgatcggca ga
//
LOCUS       DASVII010000300         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_589415,
            whole genome shotgun sequence.
ACCESSION   DASVII010000300 DASVII010000000
VERSION     DASVII010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589415"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF309_03920"
     CDS             <1..380
                     /locus_tag="VF309_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7465765.1"
                     /translation="TARFSLRSVRVAEPVAAGVALVGDAAHAVHPLAGQGVNLGFQDA
                     RVLAGLVAGRSGVERPGDLRVLRRYARERREDVTAMHFVTDGLDKLFATGGEGARAFR
                     NLGLRLVDAQPWAKAALVRRAMR"
     gene            461..1216
                     /locus_tag="VF309_03925"
     CDS             461..1216
                     /locus_tag="VF309_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX7465766.1"
                     /translation="MKFTASAAALVIATFMGIPAFAQDADKSKPDTERVKAELQKRLA
                     EAPIDGVRRIPYGGLYEVTIGGDIFYTDAKAEFLVAGTIIDLKTKENITEARIRQVNM
                     IKWESLPLDRAIKIVRGNGSRKIALFEDPNCGYCKRLHRDLQGATDLTMYVFLYPILS
                     PDSMEKSKAIWCSADPGQLWLDHMVKEAAISGDTKCATPIDQNLALGQSKKITGTPTI
                     IFENGDRVPGAIPLADLEKKLADIKLAANTHAK"
     gene            complement(1224..>1729)
                     /locus_tag="VF309_03930"
     CDS             complement(1224..>1729)
                     /locus_tag="VF309_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX7465767.1"
                     /translation="YADQNGHPFRSLGRLLIERHEIAPERASMQGIKDWARRNPLKVQ
                     DFLNSNPSYVFFRELPGDLPGPIGALGVPLTAERSIAVDPRVVPLGVPVYLATTWPNA
                     QDPLNRLMVAQDTGGAIAGGVRADFFWGFGDAAGSLAGRMRQSGRMWVLLPKGYKPPQ
                     GAETGTP"
BASE COUNT          299 a          602 c          568 g          260 t
ORIGIN      
        1 cgaccgcgcg cttcagcctg cgctcggtgc gcgtcgcaga gcccgtcgct gcgggcgtgg
       61 ccctggtggg cgacgccgcc cacgcggtgc atcccctcgc ggggcagggc gtgaacctcg
      121 gattccagga tgcaagggtc ctggccggcc tcgtcgcggg ccgctcgggt gtcgagcgcc
      181 ccggcgacct tcgcgtgctg cgccgttacg cgcgcgagcg ccgcgaggac gtgaccgcta
      241 tgcatttcgt cacggacgga ctcgacaagc tcttcgcgac cggcggcgag ggcgcgcgcg
      301 ccttccgcaa cctcggattg cgcctggtcg acgcgcagcc ctgggcgaag gcggccttgg
      361 tgcgccgcgc aatgagataa tcgttaccgg tgtagcttcc gggcggggaa ccaatcccgg
      421 ccggaagcgg cccaattttc ccattacacg gtccacgccc atgaaattca ccgcatccgc
      481 cgccgcactc gtcatcgcca cgttcatggg gattcccgcg ttcgcgcagg acgccgacaa
      541 gtcgaagccg gacaccgagc gggtcaaggc cgagctgcag aagaggcttg ccgaggcgcc
      601 gatcgacggc gtgcggcgca ttccctacgg cggcctctac gaagtgacca tcggcggcga
      661 catcttctat acggacgcca aggccgagtt cctcgtggcc gggacgatca tcgacctcaa
      721 gaccaaggag aacatcaccg aggcgcgcat tcgccaggtg aacatgatca agtgggagtc
      781 gcttccgctc gatcgcgcga tcaagatcgt gcgcggcaac ggctcgcgca agatcgccct
      841 cttcgaggat ccgaactgcg gctattgcaa gcgcctgcac cgcgacctgc aaggcgcgac
      901 cgacctcacg atgtacgtct tcctgtaccc gatcctgtcg cccgactcca tggagaaatc
      961 gaaggcgatc tggtgctcgg ccgatcccgg gcagctgtgg ctcgatcaca tggtgaagga
     1021 agcggcgatc agcggcgaca ccaaatgcgc gacgcccatc gaccagaatc tcgccctggg
     1081 ccagtcgaag aagatcaccg gcacgccgac catcatcttc gagaacggcg atcgcgttcc
     1141 gggagccatt ccgctcgccg acctcgagaa gaagctcgcc gacatcaagc tcgcggcgaa
     1201 cacgcacgcg aagtgaccac ggctcagggc gtgccggtct cggcgccctg cggcggcttg
     1261 tagcctttcg gaagcagcac ccacatcctt cccgactggc gcatcctccc cgcgaggctg
     1321 cccgcggcgt cgccgaatcc ccagaagaaa tccgcgcgca cgccgcccgc gatcgcgccg
     1381 cccgtgtcct gcgcgaccat cagccggttg agcggatcct gcgcgttggg ccacgtcgtc
     1441 gcgagataca ccggcacgcc gagaggcacg acgcggggat ccaccgcgat cgagcgctcc
     1501 gcggtgagcg gcacgccgag cgcgccgatc ggtcccggca ggtcgcctgg gagctcgcga
     1561 aagaagacgt agctcgggtt cgagttgagg aagtcctgca ccttgagcgg attgcgccgc
     1621 gcccagtcct tgatgccctg catggacgcg cgctcggggg caatctcgtg gcgctcgatg
     1681 agcaggcgcc cgagcgagcg gaagggatgc ccgttctggt ccgcatagc
//
LOCUS       DASVII010000301         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_337952,
            whole genome shotgun sequence.
ACCESSION   DASVII010000301 DASVII010000000
VERSION     DASVII010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337952"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..697)
                     /gene="hisG"
                     /locus_tag="VF309_03935"
     CDS             complement(59..697)
                     /gene="hisG"
                     /locus_tag="VF309_03935"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7465768.1"
                     /translation="MITLALAKGRIFEETAPLLAAAGIRPVEDPESSRKLILATQRAD
                     LRLILVRAADVPTYVQYGAADLGVAGRDILAEHGGQGLYQPLDLGIARCRMVVAVPEG
                     FDYAAAVKQGARLRVATKYIGTAREHFAAKGVHVDLIKLYGSMELAPLVGLADAIVDL
                     VDTGNTLKANRLRVVEEIMPVSSRLIVNPASLKLKREALAPLIEAFARLGHG"
     gene            complement(694..1944)
                     /gene="murA"
                     /locus_tag="VF309_03940"
     CDS             complement(694..1944)
                     /gene="murA"
                     /locus_tag="VF309_03940"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7465769.1"
                     /translation="MQKLSIQGGVALSGEVRVAGAKNAALPILAATLLTSDAMRVTNL
                     PQLNDVRTMVKLLERMGVKASEGEHHAELRADAITEPFAPYDLVKTMRASILALGPLT
                     ARFGEARVSLPGGCAIGERPVDLHIKGLTAMGAQIAIEAGDIHATARRLKGARIFMDT
                     VTVTGTENLMMAACLADGETVLENCAREPEVVDLARCLIAMGARIQGHGADVITVQGV
                     ERLRGVEYAVMPDRIEAGTFLAAAAATRGAITLTDAPVASLDPVLQKLREAGAQITVG
                     ETTVRIAMRARPKAVSLRTAPHPGFPTDMQAQFIAMDSVAEGTSRVTETIFENRFMHV
                     QELRRLGAHIEIEGNTAMIYGVDTLKGATVMATDLRASASLVIAGLVAEGETIIDRIY
                     HLDRGYEHLESRLASLGARIARVA"
     gene            complement(1987..2223)
                     /locus_tag="VF309_03945"
     CDS             complement(1987..2223)
                     /locus_tag="VF309_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX7465770.1"
                     /translation="MVEPSSIEQSIRAGLACTHLEVSGDGAHFDAVIVSPAFMGLSRV
                     RQHQLVYAALGDRMREEIHALSMKTFSPEEWAAR"
     gene            complement(2233..2988)
                     /locus_tag="VF309_03950"
     CDS             complement(2233..2988)
                     /locus_tag="VF309_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465771.1"
                     /translation="MSGFRTLLYKEVLRFWKVSFQTVAAPVLTTLLYLLVFAHALAGH
                     VQAFAGVTYPQFLIPGLAMMAMLQNSFANSSSSLIQSKITGNIVFILLPPLSAGEIFA
                     AYLLASMVRGLVVGTGVFAATVWLIPLPVGNPAWIAAFAIVGCGMLGTMGIIAGVWSE
                     KFDQLAAFQNFLVMPLTFLAGVFYSMHSLPPFWQSVSKLNPFFYAIDGFRYGFFGQSD
                     VSPWASLAFASAAFGALSLLTLHLIGRGYKLRH"
BASE COUNT          495 a         1062 c         1090 g          470 t
ORIGIN      
        1 gaggagcgcg tcgagcgcgg cgtcgaagcc cgaatcggcg ctcgacagcc ggcgaggctc
       61 agccatggcc cagcctcgcg aacgcctcga tcagcggcgc cagcgcttcg cgcttcagct
      121 tcagcgatgc gggattgacg atgaggcggc tcgagaccgg catgatctcc tcgaccacgc
      181 gcagccggtt cgccttgagc gtgttgccgg tatcgacaag atcgacgatc gcgtcggcca
      241 atccgacgag cggcgcgagc tccatcgacc cgtagagctt gatgaggtcg acgtgcacgc
      301 ccttcgccgc gaagtgctcg cgcgcggtgc cgatgtactt ggtggcgacg cgcaggcgcg
      361 cgccctgctt caccgccgcc gcgtagtcga agccttcggg gaccgcgacc accatcctgc
      421 atcgcgcgat gccgaggtcg aggggctggt acaggccctg gccgccatgc tcggcgagga
      481 tgtcgcggcc ggccacgccc aggtccgccg cgccgtattg cacgtaggtg ggcacgtcgg
      541 ccgcgcgcac gaggatcagg cgcaggtccg cgcgctgcgt cgcgaggatg agcttgcgcg
      601 acgactccgg gtcttccacg ggcctgatcc ccgccgcggc gagcagcggc gccgtttcct
      661 cgaagatccg ccccttggcg agcgcgagcg tgatcacgcg accctcgcga tgcgcgcgcc
      721 gaggctcgcc aggcgcgact cgagatgctc gtagccgcga tccaggtgat agatgcgatc
      781 gatgatcgtc tcgccctcgg cgacgaggcc cgcgatcacg aggctcgcgg aggcccgcag
      841 gtccgtcgcc atcacggtgg ctcccttcag cgtgtcgacg ccgtagatca tcgcggtgtt
      901 gccctcgatc tcgatgtggg cgccgaggcg gcgcagctcc tggacgtgca tgaagcggtt
      961 ctcgaagatg gtctcggtca cgcgcgaggt gccctcggcg acgctgtcca tcgcgatgaa
     1021 ctgcgcctgc atgtcagtcg gaaacccggg gtggggggcg gtgcgcaggc tcaccgcctt
     1081 cggccgcgcg cgcatcgcga tgcgcaccgt cgtctcgccc accgtgatct gcgcgcccgc
     1141 ctcgcgaagc ttctggagga cgggatcgag gctcgccacg ggcgcgtcgg tgagcgtgat
     1201 ggcgccccgg gtggccgccg ccgcggcgag gaacgtcccc gcctcgatgc gatcgggcat
     1261 cacggcgtac tcgacgcccc tcaggcgctc cacgccctgc acggtgatca cgtccgcgcc
     1321 atgtccctgg atgcgcgccc ccatcgcgat gaggcagcgc gcgagatcga ccacctcggg
     1381 ctcgcgcgcg cagttctcga gcacggtctc gccgtcggcc aggcacgcgg ccatcatcag
     1441 attctcggtg cccgtcacgg tcacggtgtc catgaagatg cgcgcgccct tgaggcgccg
     1501 ggcggtcgcg tggatgtccc cggcctcgat ggcgatctgc gcgcccatcg cggtgaggcc
     1561 cttgatgtgc aggtcgacgg gacgctcgcc gatggcgcat ccgccgggca gcgacacgcg
     1621 cgcctcgccg aagcgcgcgg tgagcggccc gagcgcgagg atcgacgcgc gcatcgtctt
     1681 cacgagatcg tagggcgcga agggctcggt gatcgcgtcg gcgcgcagct ccgcatggtg
     1741 ctcgccctcg ctcgccttca cgcccatgcg ctcgaggagc ttcaccatgg tgcgcacgtc
     1801 gttcagctgc ggcaggttcg tgacgcgcat cgcatcgctc gtgagcagcg tggccgccag
     1861 gatcggcagc gccgcgttct tcgcgccggc tacgcggacc tcgcccgaaa gcgcgacgcc
     1921 gccctgaatg gatagtttct gcatggtctc tttatgtcgg ccgggccgcg gccccgcccc
     1981 ggtctttcag cgcgcggccc attcctcggg ggaaaaggtc ttcatcgaga gggcgtggat
     2041 ctcctctcgc atgcgatcgc cgagcgccgc gtagacgagc tggtgctggc gcacgcggga
     2101 aagccccatg aaggccgggc tcacgatgac cgcgtcgaaa tgggcgccgt cgccgctcac
     2161 ctcgaggtgc gtgcaggcga ggccggcgcg gatcgactgc tctatggagc tgggttcgac
     2221 catggtgggg agtcaatgcc tgagcttgta gccgcggccg atgagatgca aggtcagcaa
     2281 tgatagggca ccgaaggcgg cgctcgcaaa cgcgaggctc gcccacggcg agacgtccga
     2341 ctggccgaag aagccgtagc ggaatccgtc gatcgcgtag aagaaggggt tcagcttcga
     2401 caccgactgc cagaagggcg gcagcgaatg catcgaatag aagacgcccg cgaggaaggt
     2461 gaggggcatc accaggaagt tctggaaggc ggcgagctgg tcgaatttct ccgaccacac
     2521 gccggcgatg attcccatgg tgccgagcat gccgcagccc acgatcgcga aagccgcgat
     2581 ccaggcggga ttgcccaccg gaagcgggat cagccacacg gtcgccgcga agacgcccgt
     2641 gccgacgacg agcccgcgca ccatcgaggc gaggaggtac gcggcgaaga tctcgcccgc
     2701 cgagagcggc ggcagcagga tgaacacgat gttgccggtg atcttcgact ggatgagcga
     2761 cgaggagcta ttggcgaacg agttctgcag catcgccatc atggcgaggc ccgggatgag
     2821 gaactgcggg taagtcaccc cggcgaacgc ctgcacgtgc cccgcgagcg cgtgggcgaa
     2881 gacgagcagg tagagcagcg tcgtgagcac cggcgccgcg accgtctgga agctcacctt
     2941 ccagaagcgc agcacttcct tgtagaggag cgtgcggaac ccgctcatcc cgccgcggcc
     3001 tcttcgcgcc gcatgatctt cacgaacact tcctcgagct ctggctcggc gacctcgagg
     3061 ctcgagaccg cggcgcccgc ctcgcgcatc gtccgcagcg cgtcctccac gtgcgcg
//
LOCUS       DASVII010000302         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_230007,
            whole genome shotgun sequence.
ACCESSION   DASVII010000302 DASVII010000000
VERSION     DASVII010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230007"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF309_03955"
     CDS             complement(<1..407)
                     /locus_tag="VF309_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465772.1"
                     /translation="MTMWLQRIEAGLGRRGSRAAVVAIALGVAAFTTGLPAGRSPRTF
                     AVLIATWLFFAGIAAGALAFVAFFRIVDADWARPLAAWITRQGSFAPVALLVLVVILA
                     GARLAPWLPPAGSGTAWLSFPILAAREVGFTVAL"
     gene            complement(404..955)
                     /locus_tag="VF309_03960"
     CDS             complement(404..955)
                     /locus_tag="VF309_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7465773.1"
                     /translation="MKRGWLALLAPAALVAVWASSALIVVGCDGMLWPASMEQQPAVQ
                     ALDEARPAPRGSIPLGGVEVLEDREDDQAVTPPRPLDAASSARGAALFAIHCIACHGR
                     EGRGDGPVSAKFPPAPNLRHVSICARSDGFIYGTLTAGGRAMPTMREGTTTRDRWDLV
                     AFVRTLQREGCIGTTAAPPGGSP"
     gene            complement(952..1455)
                     /locus_tag="VF309_03965"
     CDS             complement(952..1455)
                     /locus_tag="VF309_03965"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX7465774.1"
                     /translation="MRDPLIALFADPDSAARALQALHGENVMNARVVSSAPFPAVHET
                     GHPGPWRALGWVALVGGLTGLSCAAALEVTTSRSMDLIVGGKPIVSWTAFGVVMFELT
                     MLFAGIANFTALVVLSAFTRRSVSRRARREVTTDRLVVVVPLDGCGEAREAVIRRSLS
                     GAVEVRS"
     gene            complement(1448..>1793)
                     /locus_tag="VF309_03970"
     CDS             complement(1448..>1793)
                     /locus_tag="VF309_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465775.1"
                     /translation="PIFWLVVACNSVAPLVCFSQRARRSLPILIGVSVMVTIGMWFER
                     FLFIVTTLAHNFDPFAWRNYTPTLRELAVCLGGFGWFVMLFLLFIKIFPSMSVAETMQ
                     GEHASTVGGPHA"
BASE COUNT          332 a          638 c          620 g          203 t
ORIGIN      
        1 agagcgaccg tgaacccgac ttcgcgtgcc gcgaggatgg gaaagcttaa ccaggcggtc
       61 ccgctgcccg cgggaggcag ccacggtgcc agtcgggcgc ccgcgagaat gaccaccaac
      121 acgaggaggg cgacgggcgc aaagctcccc tgacgcgtga tccacgcggc gagcggccgc
      181 gcccagtcgg cgtcgacgat ccgaaagaat gcgacgaatg ccagtgcgcc tgcggcgatt
      241 cccgcgaaga aaagccaggt cgcaatcaac acggcgaacg tacgtggcga ccgtccggcg
      301 ggaaggccgg tcgtgaacgc cgccacgccg agcgcgatgg cgacgaccgc cgcgcgtgat
      361 ccgcgccgcc cgagccccgc ctcgatccgc tggagccaca tcgtcatggc gagcctcccg
      421 gaggcgccgc cgtggtcccg atgcacccct cgcgctggag cgtgcgcacg aaggcgacca
      481 gatcccagcg atcccgcgtg gtggtgccct cccgcatcgt gggcatggcg cggcctcccg
      541 cggtgagcgt gccgtagatg aacccgtcgc tgcgcgcgca gatcgagacg tgccgcaagt
      601 tcggcgcggg cgggaacttg gccgagaccg gtccgtctcc gcgaccctca cgaccgtggc
      661 aagcgatgca gtgaatggcg aagagcgcgg cgccgcgcgc ggacgacgcg gcgtcgagcg
      721 ggcggggagg cgtcacggcc tggtcatcct cccgatcctc gaggacctcg acgccgccga
      781 gggggatcga gccgcgcggc gccggtcgtg cctcgtcgag cgcctgcacc gccggctgtt
      841 gctccatgct ggcgggccac aacatgccgt cacatccgac gacaatgagc gccgaggacg
      901 cccagaccgc gacgagggcc gcgggcgcga ggagcgcgag ccagccgcgt ttcatgaccg
      961 aacctccacc gcgccggaca gcgagcgccg gatcaccgcc tcgcgcgcct cgccgcaccc
     1021 gtcgagtgga acgacgacga ccagccgatc ggtggtgacc tcgcgccgcg cccgacgaga
     1081 cacggaccgg cgcgtgaacg ccgacaggac cacgagcgcc gtgaagttgg cgattccggc
     1141 gaagagcatc gtgagctcga acatgaccac cccgaaggcc gtccatgaca cgatcggctt
     1201 accgcccacg atcaggtcca tcgatctcga cgtcgtgacc tcgagcgccg cggcgcaact
     1261 caggccggtg aggccgccga ccagcgcgac ccatccgagc gcccgccacg gccccggatg
     1321 accggtctcg tgcacggccg ggaacggcgc cgagctgacc acgcgcgcgt tcatcacgtt
     1381 ctcgccatgc agcgcttgca acgcgcgggc ggcgctgtcg ggatcggcga acagggcgat
     1441 gagcggatca cgcatggggc cccccgaccg tggaggcgtg ctcgccctgc atggtctccg
     1501 caaccgacat cgacgggaag atcttgatga acagcaggaa caacatgacg aaccacccga
     1561 agccgcccag gcacaccgcg agctcgcgca gcgtcggcgt gtagttgcgc caggcgaagg
     1621 gatcgaagtt gtgagcgagc gtcgtcacga tgaacaggaa ccgctcgaac cacatcccga
     1681 tggtgaccat cacggacacc ccgatcagga tcggaaggct gcgtcgcgcg cgctgggaga
     1741 aacagacgag cggcgccacg ctgttgcagg ccaccacgag ccagaagatc ggc
//
LOCUS       DASVII010000303         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_338013,
            whole genome shotgun sequence.
ACCESSION   DASVII010000303 DASVII010000000
VERSION     DASVII010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338013"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1051
                     /locus_tag="VF309_03975"
     CDS             74..1051
                     /locus_tag="VF309_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX7465776.1"
                     /translation="MKFLITDHDFPDIDLEMALLQNAGMEISAAQCRTEDDVIAAGKG
                     CAGFLTQYAPMNEKVFAALPEVRVVSRMGAGFDTVNLEDAKRRGVWVANSPDYGVGEV
                     ATHALGMAISLVRHLPFYDRDVRAGNWHYTSAGRLRRVANMTIGILGLGRIGKRMAQL
                     SQPVFGRIVACDPYIAESDFPPYVKRASLEELFRDSDVVSAHVPLNDETRGMIDLRRL
                     SLMKPGSFLVNTARGSVANLGDVLKALDSGRLEAAALDVLPKEPPEADHPVLRHPRVL
                     LTPHAAFYSIEGEKELRTKAAQNLIDWARTGRPTYVVSEGEAGFPPSRE"
     gene            1054..>1512
                     /locus_tag="VF309_03980"
     CDS             1054..>1512
                     /locus_tag="VF309_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465777.1"
                     /translation="MAAVHIRDVHKHFGGTHVIRGVTVDIADGEFAVLVGPSGCGKST
                     LLRMIAGLEEIGSGEIEIGGKVVNDMQPKERDIAMVFQNYALYPHMKVRDNMAFSMLL
                     AKRPRAEIDKRVDKAATILGLADLLDRYPRQLSGGQRQRVAMGRCIVRDPQ"
BASE COUNT          263 a          487 c          509 g          253 t
ORIGIN      
        1 tgcgacctcg acttcctcgt cggcaagagc ggcgcggacg tgccgcggga caaccattga
       61 gtcgcgagca gtcgtgaaat tcctcatcac ggaccatgat ttcccggaca tcgatctcga
      121 aatggcgttg ctgcagaatg ccgggatgga aatctccgcc gcgcagtgcc gcacggagga
      181 cgacgtgatc gcggcgggga agggatgcgc cggattcctc acgcaatacg ccccgatgaa
      241 cgagaaggtc ttcgccgcat tgccggaagt gcgtgtcgtg agccgcatgg gcgcgggttt
      301 cgacaccgtg aatctcgagg atgcgaagcg gcgcggcgta tgggtcgcca attcgccgga
      361 ctacggcgtg ggcgaggtcg ccacccacgc gctcggcatg gcgatttcgc tggtgcgcca
      421 tctcccgttc tacgatcgcg acgtgcgcgc cgggaactgg cactacacct cggcggggag
      481 gctgcgccgc gtggcgaaca tgacgatcgg catcctcggc ctggggcgca tcggcaagcg
      541 catggcgcag ctttcgcaac ccgtgttcgg ccgcatcgtg gcatgcgatc cctacatcgc
      601 ggaaagcgat tttcccccat acgtgaagcg cgcttccctc gaggagctgt ttcgcgactc
      661 cgacgtggtc tcggcgcacg tgccactcaa cgacgagact cgcggcatga tcgacctgcg
      721 caggctctcc ctcatgaagc cgggttcctt cctcgtgaac accgcgcgcg gctcggtcgc
      781 gaacctcggg gacgtgctga aggccctcga ttcgggccgg ctcgaggccg cggcgctcga
      841 cgtccttccc aaggagcctc cggaggcgga tcatccggtg ctccggcatc cgcgcgtgct
      901 gctgacgccg catgccgcgt tctactcgat cgagggggaa aaggaattga ggacgaaggc
      961 tgcgcagaac ctgatcgact gggcgaggac gggaagaccc acttacgtcg tgagcgaggg
     1021 cgaggctgga ttcccgcctt cgcgggaatg accatggcag ccgtccacat ccgcgacgtt
     1081 cacaagcatt ttggcggcac ccacgtgatc cgcggggtga ccgtcgacat cgccgacggc
     1141 gagttcgcgg tgctcgtggg gccgtcgggc tgcggcaagt cgacgctcct gcgcatgatc
     1201 gcgggactcg aggagatcgg ttccggcgag atcgagatcg gcggcaaggt cgtcaacgac
     1261 atgcagccga aggagcgcga catcgcgatg gtgttccaga actacgcgct ctatccgcac
     1321 atgaaggttc gcgacaacat ggcgttcagc atgcttcttg cgaagcgccc ccgtgccgag
     1381 atcgacaagc gcgtcgacaa ggcggccacg atcctcgggc tcgccgacct tctcgaccgc
     1441 tatccgcgcc agctctccgg cgggcagcgc cagcgggtgg ccatggggcg ctgcatcgtg
     1501 cgcgacccgc ag
//
LOCUS       DASVII010000304         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_409850,
            whole genome shotgun sequence.
ACCESSION   DASVII010000304 DASVII010000000
VERSION     DASVII010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409850"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VF309_03985"
     CDS             complement(<1..922)
                     /locus_tag="VF309_03985"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX7465778.1"
                     /translation="MVTLKDIESAAEAIRGQVPATPCMHSRTLSDITGAEVYLKFENH
                     QFTASFKERGALNKLLSLDAAQRAKGVIACSAGNHAQGVAYHANRLGIPSVIVMPRYT
                     PWVKVEQTQRHGAEVILEGDNFDGAKDFALEAAVERGLTLVHPYNDEKIIAGQGTAGV
                     EILEDHPTLDALIVAIGGGGLISGISIAAKAMKPAIEVYGVEMLRFPGMYHAMKGTQA
                     QFAASTIAEGIAVKEPGALTLPIIRENVSEIFLVDEGDIEEAIVMLLEIEKTVVEGAG
                     AAPLAALLANKARFAGKRVGLVLGGGNIDPM"
     gene            complement(954..>1502)
                     /locus_tag="VF309_03990"
     CDS             complement(954..>1502)
                     /locus_tag="VF309_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7465779.1"
                     /translation="VRHGEHDHLLTADAYVVALGSYSPLLTRPIGLSLPVYPAKGYSA
                     SLPIRDASKAPTVSLTDDGAKIVITRLGNRMRIAGTAELSGYSTELNPVRCEALTRRV
                     GEMFPDAGEYDKATFWTGLRPSTPSNVPLVGATRFPNLYLNTGHGTLGWTMAAGSGRA
                     LADIISGRKPDVDFKFTVTPAK"
BASE COUNT          216 a          489 c          536 g          261 t
ORIGIN      
        1 tcatcgggtc gatgttgccg ccgccgagca ccagccccac gcgttttccc gcgaagcgcg
       61 ccttgttggc gaggagcgcc gcgagcgggg cggcgcccgc gccctccacc acggtcttct
      121 cgatctcgag gagcatcacg atcgcctcct cgatgtcgcc ctcgtcgacg aggaagatct
      181 cggagacgtt ctcgcggatg atgggcagcg tgagcgcgcc cggctccttc acggcgatcc
      241 cctcggcgat ggtcgaggcg gcgaactgcg cctgggtgcc cttcatcgcg tgatacatcc
      301 ccgggaagcg cagcatctcg acgccgtaga cctcgatcgc gggcttcatc gccttcgcgg
      361 cgatcgagat tcccgagatg agcccgccgc cgccgatcgc gacaatgagc gcgtcgagcg
      421 tgggatgatc ctcgagaatc tcgacgccgg cggtcccctg cccggcgatg atcttctcgt
      481 cgttgtacgg gtgcaccagg gtcagcccgc gctcgaccgc cgcctcgagc gcgaagtcct
      541 tggcgccgtc gaagttgtcg ccctcgagga tcacctccgc gccgtggcgc tgggtctgct
      601 cgaccttgac ccacggcgta tagcgcggca tcacgatgac cgaggggatg ccgagccggt
      661 tcgcgtgata ggcgacccct tgcgcgtggt tgccggccga gcaggcgatg acgcccttgg
      721 cgcgctgggc cgcgtcgagc gagaggagct tgttgagggc gccgcgctcc ttgaacgatg
      781 cggtgaactg gtggttctcg aacttgaggt agacctcggc gccggtgatg tcggagagcg
      841 tgcgcgaatg catgcacggc gtcgccggga cctgcccgcg gatcgcttcg gcggcgctct
      901 cgatgtcttt cagcgtgacc atgattggac ccccgcctac gcgggggtga cggttatttc
      961 gcgggggtga cggtaaattt gaagtccacg tcgggctttc gaccgctgat gatatccgca
     1021 agcgcgcgcc ccgacccggc ggccatggtc cagccgagcg tgccgtggcc tgtgttgagg
     1081 tagaggttcg ggaagcgcgt ggcgcccacg agcgggacgt tggagggcgt ggagggcctc
     1141 aagccggtcc agaacgtggc cttgtcgtac tcgcccgcgt ccgggaacat ctcgcccacg
     1201 cggcgcgtga gcgcctcgca gcgcaccggg ttcagctccg tcgagtagcc ggaaagctcc
     1261 gccgtgccgg cgatgcgcat gcggtttccg aggcgcgtga tgacgatctt ggcgccgtcg
     1321 tcggtaaggc tcacggtggg cgccttcgac gcgtcgcgga tcggcagcga tgccgaatag
     1381 cccttggcgg gatagaccgg caggctcaat ccgatcgggc gcgtgaggag cggcgagtag
     1441 ctgccgagcg cgaccacgta ggcgtcggcc gtgagcaggt gatcgtgctc gccgtggcgc
     1501 ac
//
LOCUS       DASVII010000305         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_409888,
            whole genome shotgun sequence.
ACCESSION   DASVII010000305 DASVII010000000
VERSION     DASVII010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409888"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF309_03995"
     CDS             complement(<1..359)
                     /locus_tag="VF309_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465780.1"
                     /translation="MPFISPGLCKNAASTIRVIRRAVIDPSPQPDSRYAVMRLMVTLV
                     IMTIGASGMYVVPVMLPAVQAEFGVARADASMPYSLLMIGFGVGGLLMGRLADRFGVM
                     VPLVLGAVGLGAGMVTAG"
     gene            353..715
                     /locus_tag="VF309_04000"
     CDS             353..715
                     /locus_tag="VF309_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX7465781.1"
                     /translation="MATYVDGFIVPVPKRKLAAYRAMAKRAGKVWREHGALEYRECIA
                     EDVKVGKRTSFPRSVKLKPGETVVFSWIVYKSRAQRDRIMAKVMKDPRLADMMDPKNV
                     PFDGKRMIYGGFELFVDL"
     gene            736..1815
                     /locus_tag="VF309_04005"
     CDS             736..1815
                     /locus_tag="VF309_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX7465782.1"
                     /translation="MQNKSAGRRDHRKTATRAGLVYVTDGAAGIRRKRAGSGWSYFAP
                     NGVRIRDPEKRRRINSLAIPPAWTDVWICPDPAGHIQATARDARGRKQYRYHASYREA
                     CDRSKCRHMLEFSEILPDLRERVERDLRAEDLSRRQVLATVIRLLDRTLIRVGNDEYA
                     RENQSFGLTTLRRRHVQVDGTMLRFTFRGKSGIEHSVALTDRRLARIVQRCQDLPGTE
                     IFQYLDAAGKRQSIGSDDVNTWLQEITGRHITAKDFRTWGGTMHAAIALHEMGTAATR
                     READRNIVRAIDGVSERLGNTRAVCRKYYVHPALIEAYLEGRTVRLPPPPPKTSKPAP
                     RGERAAALRRDEVAVLQFLNDELGT"
BASE COUNT          340 a          659 c          642 g          263 t
ORIGIN      
        1 cccgcggtca ccattcccgc gccgaggccc accgcgccga ggacgagcgg caccatcacg
       61 ccgaagcggt cggcgaggcg ccccatgagg aggccgccca cgccgaagcc gatcatgagc
      121 agcgaatacg gcatcgaggc gtccgcgcgc gccacgccga attccgcctg caccgcggga
      181 agcatcaccg gcaccacgta catgccgctc gcgccgatcg tcatgatgac gagcgtcacc
      241 atgaggcgca tcacggcgta gcgggaatcg ggctggggac tcgggtcgat gacggctcgg
      301 cgaatgacgc gtatcgtgga tgcggcattc ttgcacaacc cgggagaaat gaatggcaac
      361 ctacgtcgac ggtttcatcg tacctgttcc gaagaggaag ctcgccgcct atcgcgcgat
      421 ggcgaaacgc gccgggaagg tgtggcgcga gcacggcgcc ctcgaatacc gcgagtgcat
      481 cgccgaggac gtgaaggtcg ggaagcgcac ctcgtttccg cgcagcgtga agctcaagcc
      541 cggcgagacc gtggtgttct cctggatcgt gtacaagtcg cgcgcgcagc gcgaccggat
      601 catggcgaag gtgatgaagg acccgcgcct cgccgacatg atggatccga agaacgtgcc
      661 cttcgacggc aagcggatga tctacggcgg attcgagctc ttcgtcgatc tctgaccccc
      721 acggcggcga gcccgttgca gaacaagagc gccgggaggc gcgaccatcg caagaccgcg
      781 acgcgcgccg gcctcgtcta cgtgaccgac ggcgccgcgg gcatccggcg caagcgcgcg
      841 ggaagcggct ggtcctactt cgcgcccaac ggcgtgcgca tccgcgatcc ggaaaagcgc
      901 cgccgcatca actccctcgc cattccgccg gcgtggaccg acgtctggat ctgccccgat
      961 ccggcgggcc acatccaggc gacggcccgg gacgcgcgcg ggcgcaagca ataccgctac
     1021 cacgcgtcgt atcgcgaggc ctgcgaccgc agcaagtgtc gccacatgct cgagttcagc
     1081 gagatcctgc cggacctgcg cgagcgggtg gagcgggacc tccgcgccga agacctctcg
     1141 cggcgccagg tcctcgcgac ggtgatccgc ctcctcgaca ggaccctcat ccgcgtcggc
     1201 aacgacgagt acgcgcgcga gaaccaatcg ttcgggctca cgacgctacg gcgccggcac
     1261 gtgcaggtgg atggcacgat gctgcgcttc acgttccgcg gcaagagcgg catcgagcat
     1321 tccgtcgcgc tcacggaccg gcgcctcgcg cgcatcgtgc agcgctgcca ggacctgcct
     1381 ggcaccgaga tcttccagta cctcgacgcg gcgggaaagc gccagtcgat cggctccgac
     1441 gacgtgaaca cgtggctgca ggagatcacc ggccgccaca tcaccgcgaa ggactttcgc
     1501 acctggggag gcacgatgca cgcggcgatc gcgctccacg agatgggcac cgccgcgacg
     1561 cgccgcgagg ccgaccgcaa tatcgtgagg gcgatcgatg gcgtttccga gcgcctcggc
     1621 aacacgcgtg ccgtgtgccg caagtactac gtgcatccgg cgttgatcga ggcctacctc
     1681 gaggggcgca cggtgcgcct gccgcctcct ccaccgaaga cttcgaagcc cgcgccgcgc
     1741 ggcgagcggg cggcggcgct gcgccgcgac gaggtggcgg tgctgcagtt cctgaacgac
     1801 gagctcggca cgtgagggca gggctcagga ggagcgtcgc ttggcgttct tccagtgctc
     1861 tcgccgacgt gtctccgaaa cgaattcttc cagctcggga tcca
//
LOCUS       DASVII010000306         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_230172,
            whole genome shotgun sequence.
ACCESSION   DASVII010000306 DASVII010000000
VERSION     DASVII010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230172"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..697
                     /gene="adk"
                     /locus_tag="VF309_04010"
     CDS             44..697
                     /gene="adk"
                     /locus_tag="VF309_04010"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7465783.1"
                     /translation="MKLILLGPPGAGKGTQATFLTKHYGIPQISTGDMLRAAVKAGTP
                     LGLAAKQVMDGGFLVSDDIIIGLVKERIKAPDCAGGFLFDGFPRTIPQAEAMRDSGVR
                     LEHVVEIAVPDAVIIERMSGRRVHQPSGRSYHVKYNPPKNDMKDDVTGEHLIQRVDDK
                     EETVKNRLGIYHDQTEPLVEFYEKWHGAGDPGAPRYDRVDGIGSVDEVRERVFAALG"
     gene            716..>1878
                     /locus_tag="VF309_04015"
     CDS             716..>1878
                     /locus_tag="VF309_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX7465784.1"
                     /translation="MPRNAFYAQSGGVTAVINASAAGVIETARKHARQIGKVYAGRNG
                     IIGALTEDLIDTSREPAANIRALLHTPGGAFGSARYKLKGVEKGGLEYERLIEVFKAH
                     DIGYFFYNGGNDSADTCLKVSQLSEKMGYPMVAVHVPKTVDNDLPITDTCPGFGSVAK
                     YVATSMMEAVLDVESMAKTSTKVFILEVMGRHAGWITAATGLAQRGAGQGPHILLFPE
                     VPWNEEAFLARVDAMVKAHGYCAVGASEGIQDRDGKFLSESGLKDAFGHSQLGGVAPM
                     LANLVQSKLKYKQHWAVADYLQRAARHIASKTDLEQSYAVGRAAVEFAAKGMSGVMPT
                     IVRTSSRPYRWKIGVAKLTDVANVEKKMPRDFITADGFHITAKCRNYLQPLISG"
BASE COUNT          346 a          617 c          634 g          281 t
ORIGIN      
        1 tggacacgcc gcaggatctc gaggccgtgc gtaaaatatt cccatgaagc tcattcttct
       61 tgggccgccg ggcgctggca agggcacgca agcgaccttc ctgaccaagc actacggcat
      121 cccgcagatc tccaccggcg acatgctgcg agccgcggtg aaggcgggaa cgccgctcgg
      181 tctcgcggcc aagcaggtga tggatggcgg cttcctcgtc tccgacgaca tcatcatcgg
      241 actcgtgaag gagcgcatca aggcgcccga ctgcgccggc gggtttctct tcgacggctt
      301 tccccgcacg atcccgcagg cggaggcgat gcgcgattcc ggcgtgcgcc tcgagcacgt
      361 ggtcgagatc gcggtccccg acgcggtgat catcgagcgc atgtcggggc ggcgcgtgca
      421 ccagccctcg gggcgctcct atcacgtgaa gtacaacccg ccgaagaacg acatgaagga
      481 cgacgtcacg ggcgaacacc tcatccagcg cgtggacgac aaggaggaga cggtgaagaa
      541 ccgcctcggc atctaccacg accagacgga gcccctcgtc gagttctacg agaagtggca
      601 cggggccggc gatcccggcg cgccccggta cgaccgtgtc gacggcatcg ggtccgtcga
      661 cgaggtgcgc gagcgcgtct tcgcggctct cggctagggc ggggcgcgaa tcgccatgcc
      721 acgcaacgct ttctatgcgc agtccggcgg cgtgaccgcc gtcatcaacg cgagcgccgc
      781 tggagtgatc gagaccgcgc gcaagcacgc gaggcagatc ggcaaggtct acgcgggcag
      841 gaacggcatc atcggcgcgc tcaccgaaga tctcatcgac accagccggg agcccgccgc
      901 gaacatccgc gcgctcctgc acacgccggg cggcgccttc ggctcggcgc gctacaagct
      961 caagggcgtg gagaagggcg gcctcgagta cgagcgcctc atcgaggtct tcaaggcgca
     1021 cgacatcggc tacttcttct acaacggcgg caacgattcc gccgacacct gcctcaaggt
     1081 gtcgcagttg agcgagaaga tgggctatcc gatggtcgcg gtgcacgtgc ccaagaccgt
     1141 cgacaacgat ttgccgatca ccgatacgtg cccgggcttc ggctcggtgg cgaagtacgt
     1201 cgcgaccagc atgatggagg ccgtgctcga cgtggagtcg atggcgaaga cttccacgaa
     1261 ggtgttcatc ctcgaggtga tggggcgcca cgcgggatgg atcaccgcgg ccacgggcct
     1321 cgcgcagcgc ggcgccgggc aggggccgca tatcctcctc tttcccgagg tgccctggaa
     1381 cgaggaggcg ttcctcgcgc gcgtcgacgc gatggtgaag gcgcacggct actgcgccgt
     1441 cggcgcttcc gaaggcatcc aggacaggga cggcaagttc ctttcggaat cgggactgaa
     1501 ggacgcgttc ggccactcgc agctgggcgg cgtggcgccc atgctcgcga acctcgtgca
     1561 gtcgaagctc aagtacaagc agcactgggc cgtggccgac tacctgcagc gggccgcgcg
     1621 ccacatcgcc tcgaagacgg acctcgagca gtcgtacgcg gtgggcaggg ccgcggtcga
     1681 attcgccgcg aagggcatgt cgggcgtgat gccgaccatc gtgcgcacgt cgagcaggcc
     1741 ctatcgctgg aagatcggcg tcgcgaagct gaccgacgtc gccaacgtcg agaagaagat
     1801 gccgcgcgac ttcatcaccg cggacggctt ccacatcacg gcgaagtgcc gcaattatct
     1861 gcagccgctc atctcggg
//
LOCUS       DASVII010000307         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_445721,
            whole genome shotgun sequence.
ACCESSION   DASVII010000307 DASVII010000000
VERSION     DASVII010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445721"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF309_04020"
     CDS             complement(<1..259)
                     /locus_tag="VF309_04020"
                     /inference="COORDINATES: protein motif:HMM:NF037200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HEX7465785.1"
                     /translation="MSAPRLLLYCRAGFEKECAQEITATAAAGVTGFVKGRPDSGFAI
                     FQAHDDDTGAELGKHIEFRRLVFPRQLVRTGELLALAEGDRV"
     gene            complement(256..1032)
                     /locus_tag="VF309_04025"
     CDS             complement(256..1032)
                     /locus_tag="VF309_04025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7465786.1"
                     /translation="MLVDSHCHLDFPELHADLPGVMSRMSDNGVTHALTISTTLQTFP
                     AVREVARSRPNLWCTAGVHPDELRDGREASIDELLELADDPKVVGIGETGLDYFRVEG
                     DTEWQRERFRTHIRAARRCGKPLVIHTRASADDTLRIMGEEAAGEVGGVMHCFTESWE
                     VAEAAMAQGFHISFSGIVTFKNAKDLKEVARRVPLERMLVETDSPYLAPVPHRGESNQ
                     PAYVRHVAEEIARLRGITLEEVAAATTANFFRLFGHARPA"
     gene            complement(1088..>1951)
                     /locus_tag="VF309_04030"
     CDS             complement(1088..>1951)
                     /locus_tag="VF309_04030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7465787.1"
                     /translation="YDVSDEFYALWLDPRMVYSCGYFRRDDDALEAAQLQKLDHICAK
                     LRLEPGETFLDIGCGWGALVLRAAERYGVKATGITLSENQHRLANERIRAAGLEDRCK
                     VVLQDYRDAPGEGVYDKIASVGMFEHVGLRNLPVYFSTVRRLLKEGGLFLNHGITTSD
                     TRNRAMGLGAGEFIDRYVFPRGELPHLHRAVLEMSEQNLEVHDVECLRTHYAKTLELW
                     SANYERNVDAAVAASNQRTARIWRLYLAGCAYAFEQGWVSIYQVLGSKQTKPGRTSLS
                     LTREWMYRGEI"
BASE COUNT          303 a          664 c          664 g          320 t
ORIGIN      
        1 atacgcggtc gccttcggcg agagccagga gctcgcccgt gcgcacgagc tgtcgcggga
       61 acaccaggcg cctgaactcg atgtgcttgc cgagctcggc gcccgtgtcg tcgtcatgcg
      121 cctggaagat cgcgaagccg ctgtcggggc gccccttcac gaagcccgtg acgcccgccg
      181 ccgcggtcgc ggtgatctcc tgggcgcact ccttctcgaa gcccgcgcgg cagtagagga
      241 gcagccgcgg cgcgctcacg ccggccgcgc gtggccgaag aggcggaaga agtttgcggt
      301 ggtggccgcg gcgacctctt cgagcgtgat gcctcgcagg cgcgcgatct cctcggcaac
      361 gtgcctcaca taggcgggct ggttcgactc gccgcgatgc ggcacgggcg cgaggtacgg
      421 cgaatcggtc tcgaccagca tgcgctcgag gggaacgcgc cgggcgacct ccttcaggtc
      481 cttcgcgttc ttgaaggtca cgatgccgga gaaggagatg tgaaagccct gcgccatcgc
      541 cgcttcggcc acctcccacg attccgtgaa gcaatgcatg acgccgccca cctcccccgc
      601 ggcctcctcg cccatgatcc gcagcgtgtc gtcggccgaa gcacgggtgt ggatcaccag
      661 cggcttgccg caacggcgcg cggcgcggat gtgggtgcgg aagcgctcgc gctgccactc
      721 ggtgtcgccc tcgacgcgaa agtaatcgag cccggtctcg ccgatgccca ccaccttcgg
      781 atcgtccgcg agctcgagga gctcgtcgat gctcgcttcg cgcccgtcgc ggagctcgtc
      841 cggatgcacg ccggcggtgc accagaggtt gggccgcgac cgcgcgacct cgcgcacggc
      901 gggaaaagtc tgcagcgtgg tgctgatggt gagcgcgtgg gtgacgccgt tgtcgctcat
      961 tcgcgacatg acgccgggca ggtcggcgtg cagctccggg aaatcgaggt ggcagtggga
     1021 atcgacgagc atgcgtgtga ttctaccggc gcgagcagcg gccggcatcc gaaagacgcg
     1081 cgccgaacta gatctcgccg cggtacatcc actcccgtgt gagcgacagc gacgtgcgcc
     1141 cgggcttcgt ctgcttcgat ccaagcacct ggtagatcga cacccatccc tgctcgaagg
     1201 cgtacgcgca tccggcgagg tacagccgcc agatgcgcgc ggtgcgctgg ttggacgccg
     1261 cgaccgccgc gtcgacgttc ctttcgtaat tcgcgctcca gagctccagc gtcttcgcgt
     1321 agtgcgttcg caggcactcc acgtcgtgga cctcgaggtt ctgctcgctc atctctaaca
     1381 cggcccggtg caggtgggga agctcgcccc tcgggaatac gtagcggtcg atgaattcgc
     1441 cggcgccgag gcccatggcc cggttgcgcg tatcggacgt ggtgatgccg tggttgagga
     1501 agagcccccc ctccttgagc agcctgcgca ccgtgctgaa atacacgggc aggttgcgca
     1561 gccccacgtg ctcgaacatc cccacgctcg cgatcttgtc gtagacgccc tcgccgggcg
     1621 cgtcgcggta gtcctgcagg acgaccttgc agcgatcctc gagtcccgcc gcgcgaatgc
     1681 gctcgttggc gaggcgatgc tggttctcgg agagcgtgat gcccgtcgcc ttcactccat
     1741 agcgctcggc ggcgcgcagc acgagcgcac cccagccgca cccgatgtcg aggaaagtct
     1801 cgccgggctc gaggcgcagc ttggcgcaga tgtggtcgag cttctgcagc tgcgccgcct
     1861 cgagcgcgtc gtcgtcgcgg cggaagtagc cgcacgagta caccatccgg ggatcgagcc
     1921 acagggcata gaactcgtcg gagacatcat a
//
LOCUS       DASVII010000308         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_553918,
            whole genome shotgun sequence.
ACCESSION   DASVII010000308 DASVII010000000
VERSION     DASVII010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553918"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF309_04035"
     CDS             <1..291
                     /locus_tag="VF309_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7465788.1"
                     /translation="ESDRIVTIEGLAPNATHAVQRAWAEIDVPQCGYCQSGQIMAAAA
                     LLSANAHPSDADIEAAMTNICRCGTYQRIRTAVHRAAELKLAEGGSQDKAKA"
     gene            306..>2096
                     /locus_tag="VF309_04040"
     CDS             306..>2096
                     /locus_tag="VF309_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7465789.1"
                     /translation="MKKTSNTPNQSRRQFIVGSAAAGGGLALGLHVPFGLAQDARAAA
                     PAEVNLWVAIRPDETCIVRIARAEMGQGTLTGLAQLVAEELECDWSKIATEPITPGRN
                     LAAKRAWGEMGTGGSRGIRTSNDYVRQGGAAARMMLLQAAADQWQVPVGELSVEKGVI
                     RHAASGRSTTYGQVAPAAAKLSPPDAKSIKLKDARLWKVAGQPLKRLDTADKLDGSKV
                     FSIDVQLPGMLNAAIKQCPVFGGKVVSYDESKIAGRRGVKGVVKVDEGSVAVVADTWW
                     RAKKALDALPIVWDEGPSANVTSASIAEHLKGGLTATNAFALRNEGDALKAIEGAAKR
                     VEAIYSTPFLAHATMEPMNCTARIGKDRAEVWVPTQNAEASLAALSEASGLPLAQCEV
                     NRTDLGCGFGRRGGNQDYTTQATAIAKHFPGVPVKLIWSREEDVQHDFYRPISQCKLA
                     AGLDDKGNVTGMHIRISGQSINAFVNPASIADGKDKRQLQGMWAEPGDAQLGYEFPNF
                     LLEYAMRNTHVPVGPWRGVNTNQNGVYLECFMDEVARAAGKDPLEFRRQYMAKFPKHL
                     AVLNAAAEKAGWGTPLPAGHYRGIAQFMGYF"
BASE COUNT          379 a          708 c          726 g          283 t
ORIGIN      
        1 gaaagcgatc gtatcgtcac gatcgagggc ctcgcgccga acgcgaccca cgccgtgcag
       61 cgcgcttggg cggagatcga cgtcccgcag tgcggctact gccagtcggg ccagatcatg
      121 gcggccgccg cgctcctctc cgccaacgcc catccgagcg acgccgacat cgaggccgcg
      181 atgaccaaca tctgccgctg cggcacctac cagcgcattc gcaccgccgt gcaccgggcc
      241 gctgaattga agttggccga gggcggttcc caggacaagg cgaaagcctg agcgcggagg
      301 cgaccatgaa gaaaacttcc aacacgccga atcaatcccg ccgccagttc atcgtgggct
      361 ccgccgcggc gggtggaggg ctggccctgg gcctgcacgt gcctttcggg ctcgcgcagg
      421 atgcgcgcgc cgctgcgccg gccgaggtca acctgtgggt cgcgattcgt cccgacgaga
      481 cttgcatcgt ccgcatcgcg cgcgccgaga tggggcaggg caccctcacc ggcctcgcgc
      541 agctcgtcgc cgaggagctc gagtgcgatt ggagcaagat cgcgaccgag cccatcacgc
      601 ccggacgcaa cctcgccgcg aagcgcgcgt ggggcgagat gggaacgggc ggcagccgcg
      661 gcattcgcac gtcgaacgat tacgtgcgcc agggcggcgc cgccgcgaga atgatgttgc
      721 tccaggccgc agcggaccaa tggcaggttc ccgtgggcga gctcagcgtg gagaagggcg
      781 tcatccgtca cgcggcgtcg ggccgcagca ccacgtatgg ccaggtcgcg ccggccgcgg
      841 cgaagctgag ccctcccgat gcgaagagca tcaagctcaa ggacgcgcgc ctctggaagg
      901 tcgcgggcca gccgctcaag cgcctcgaca ccgccgacaa gctggacggc agcaaggtct
      961 tttcgatcga cgtgcagttg cccggcatgc tcaacgcggc catcaagcaa tgcccggtct
     1021 tcggcggcaa ggtcgtgagc tacgacgagt cgaagatcgc gggccgccgc ggcgtgaagg
     1081 gggtcgtcaa ggtcgacgag ggctccgtcg cggtcgtcgc cgacacctgg tggcgcgcga
     1141 agaaggcgct cgacgcgttg ccgatcgtct gggacgaggg gccgagcgcg aacgtcacga
     1201 gcgcgtcgat cgccgagcat ttgaagggcg gcctcaccgc caccaacgcg ttcgcgcttc
     1261 gcaacgaagg cgacgcgttg aaggcgatcg agggcgccgc gaagagagtc gaggccatct
     1321 acagcacgcc gttcctcgcc cacgccacca tggagccgat gaactgcacg gcgcgcatcg
     1381 gaaaggatcg cgccgaagtc tgggtgccga cgcagaatgc ggaagcctcg ctcgccgcgc
     1441 tttccgaggc gtccggcctg ccgctcgcgc agtgcgaggt gaatcgcacc gacctcggct
     1501 gcggcttcgg ccgccgcggc ggcaaccagg actacacgac ccaggcgacc gccatcgcga
     1561 agcatttccc tggcgtgccg gtcaagctca tctggagccg cgaagaggat gtgcagcacg
     1621 acttctatcg gccgatctcg cagtgcaagc tcgccgcggg gctcgacgac aagggcaacg
     1681 tcacggggat gcacatccgc atctcgggcc agtcgatcaa cgcattcgtg aacccggcga
     1741 gcatcgccga cggcaaggac aagcgccagc tgcagggcat gtgggcggag ccgggcgacg
     1801 cgcagctcgg gtacgagttt ccgaacttcc tgctcgagta cgcgatgcgc aacacgcacg
     1861 tgcccgtcgg tccctggcgc ggggtgaaca cgaaccagaa cggcgtctac ctcgaatgct
     1921 tcatggacga ggtcgccagg gcggcgggca aggacccgct cgaattccgc cgccagtaca
     1981 tggcgaagtt cccgaagcac ctcgcggtgc tgaatgccgc ggcggaaaag gcgggatggg
     2041 gcacgccgct tccggcgggg cactaccgcg gcatcgcgca gttcatgggc tacttc
//
LOCUS       DASVII010000309         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_374333,
            whole genome shotgun sequence.
ACCESSION   DASVII010000309 DASVII010000000
VERSION     DASVII010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374333"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1838
                     /locus_tag="VF309_04045"
     CDS             141..1838
                     /locus_tag="VF309_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX7465790.1"
                     /translation="MIAISPDIRKKASDVCPAFAAQLEGLAIHADDAVEFMQNAIHYG
                     YLDRDTAGEIIAEAIGRTHVNLGKTLFQQDLIDKVPYELASTKKVIPLYKMGAVVTVA
                     MVDPLDKKLISALELFLGSKVSPVFSFPDEIESAIVVKYHSPLEFDKLVDEFDFAPFL
                     HADLTDAHLSELMQMSELVGMSDSLILMALKERASDIHIEPKKHDLLVRFRVDGSLHE
                     RMVLPKNLALPLVSRFKIISGMDITNRRTPQDGRINFPLPIKNIDIRLSSLPTIHGEK
                     LVLRMLGSPFAGTSLNLETLDIVPSLLTRLKQVIRQPNGILFVTGPTGCGKTTTLYAA
                     LNFINRPELNVITLEDPVEYEWPSINQVQVDEKAGRSFHALLRSVLRQDPDVILVGEI
                     RDTETARIATQAAMTGHLVLTTLHTNDAIQASTRLMDMGVERFMVAPSLIGVLNQRLV
                     RRICPFCRVEHSPGEKNLARFFTWKAGAKLPPFFRGDGCEHCNRTGYLGRIAIHEFLY
                     ITHRLRDGLLGQLNYNQLLAIANSEGFHEMRYDGYKKALRGLTTLEEVIEATMPETE"
BASE COUNT          373 a          702 c          571 g          308 t
ORIGIN      
        1 ttcgccgact tcgtccgcta cgacctggag gcgctcgcgg tcgaagccgc gcaggagaag
       61 gcgttcgacg agtcggcccg cgtgcgcgtg acccagcagg acatccgcaa gctgctcgag
      121 aagcgcagga agggccacaa atgatcgcca tcagccccga catccgcaag aaggcgagcg
      181 acgtctgccc ggcgttcgcc gcgcagctcg aaggcctcgc gatccatgcc gacgacgcgg
      241 tcgaattcat gcagaacgcg atccactacg gctacctcga ccgcgacacc gcgggagaaa
      301 tcatcgccga agccatcggc cgcacccacg tgaacctcgg gaagaccctc ttccagcagg
      361 acctcatcga caaggttccc tacgagctcg cgagcaccaa gaaggtgatc ccgctctaca
      421 agatgggagc cgtcgtcacg gtcgcgatgg tcgacccgct cgacaagaag ctcatcagcg
      481 cgctcgagct cttcctcggg tcgaaggtaa gccccgtgtt cagcttcccc gacgagatcg
      541 agtcggcgat cgtcgtgaag taccactccc cgctcgagtt cgacaagctg gtggacgaat
      601 tcgacttcgc gccgttcctg cacgcggacc tcaccgacgc gcatctgtcg gagctgatgc
      661 aaatgagcga gctcgtgggc atgtcggact cgctgatcct catggcattg aaggagcgcg
      721 cctccgacat ccacatcgag ccgaagaagc acgacctcct ggtccgcttc cgcgtggacg
      781 gctccctcca cgagcgcatg gtgctgccga agaacctcgc gctccccctc gtctcgcgct
      841 tcaagatcat ctccgggatg gacatcacca accggcgcac gccccaggac gggcggatca
      901 attttccgct gcccatcaag aacatcgaca tccgcctgtc gagcctgccc accatccacg
      961 gcgagaagct ggtgctgcgc atgctcgggt cgccgttcgc cggaacctcg ctcaatctcg
     1021 agaccctcga catcgtgccc tcgcttctca cgcgcctgaa gcaggtgatc cgccagccca
     1081 acgggatcct cttcgtgacc gggccgaccg ggtgcggaaa gaccacgacg ctctacgcgg
     1141 cgctcaactt catcaatcgc ccggagctga acgtcatcac cctcgaggat ccggtcgaat
     1201 acgagtggcc gagcatcaac caggtgcagg tcgacgaaaa ggcggggcgc tcgttccacg
     1261 cgctgctgcg ctcggtgctg cgccaggacc cggacgtgat cctggtgggc gagatccgcg
     1321 acacggagac ggcgcgcatc gcgacccagg ccgcgatgac cggccacctc gtgctcacca
     1381 cgctgcacac caacgacgcg atccaggcct cgacgcgcct catggacatg ggcgtcgagc
     1441 gcttcatggt cgcgccctcc ctcatcgggg tgctcaacca gaggctggtg cgcaggatct
     1501 gcccgttctg ccgcgtcgag cattcccccg gcgagaagaa tctcgcgcgc ttcttcacct
     1561 ggaaggcggg cgccaaattg ccgcccttct tccgcggcga cggctgcgag cactgcaacc
     1621 gcaccggcta cctcggccgc atcgcgatcc acgaattcct ctacatcacg caccggctgc
     1681 gcgacggcct gctgggacag ctcaactaca accagcttct cgccatcgcc aactccgagg
     1741 gcttccacga gatgcgctac gacggctaca agaaggcgct gcgcggcctc accactctcg
     1801 aggaagtgat cgaggcgacc atgcccgaga ccgagtgatg gaacccgccg cccgcgccga
     1861 attcctgcgc ctgctcgagg tgcagcgcgc cggctacctc gaggcccttc cgctcaagct
     1921 cgacgaagtg actctcctct ggagcggcat cgcg
//
LOCUS       DASVII010000310         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_697504,
            whole genome shotgun sequence.
ACCESSION   DASVII010000310 DASVII010000000
VERSION     DASVII010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697504"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="adk"
                     /locus_tag="VF309_04050"
     CDS             complement(<1..508)
                     /gene="adk"
                     /locus_tag="VF309_04050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7465791.1"
                     /translation="MRLILLGGPGAGKGTQATFITQKYRIPQISTGDMLRAAVKAGTP
                     LGLAAKKVMDGGFLVSDDIIIGLVKERIKAPDCASGFLFDGFPRTIPQAQAMRDSGVR
                     LEHVVEIAVPDAVIIERMSGRRVHQPSGRSYHVKYNPPKNDMKDDITGEALIQRVDDK
                     EETVKNRLG"
     gene            complement(547..1317)
                     /gene="kdsB"
                     /locus_tag="VF309_04055"
     CDS             complement(547..1317)
                     /gene="kdsB"
                     /locus_tag="VF309_04055"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX7465792.1"
                     /translation="MERLAFKVVIPARMRSTRLPGKMLADIAGKPLVVWVVERALASG
                     ADEVVVATDHSDIGDAVAAHGHKVCTTSTSHETGTDRLAEAVALLGLKPDEIVVNLQG
                     DEPLIDPALIRKVASELALRPNASISTAVHPITSAQAFFDPNVVKVVIDTEGYAQYFS
                     RAPIPYARDAFAKTRDILPEGFAAYRHIGLYASRVRFLRESSSLSPTVAERFEALEQL
                     RALGHGHRIAVAFWNEPMEAGVDTREDLERVRAKLGHV"
     gene            complement(1325..1504)
                     /locus_tag="VF309_04060"
     CDS             complement(1325..1504)
                     /locus_tag="VF309_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX7465793.1"
                     /translation="MDTKLLEILVCPLCKGPLLWRKEASELICKGDRLAFPVKDDIPV
                     MLEEEARTLRPEEEP"
     gene            complement(1485..1640)
                     /locus_tag="VF309_04065"
     CDS             complement(1485..1640)
                     /locus_tag="VF309_04065"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX7465794.1"
                     /translation="MTEKDAVKCAAFADSRMWYLRVDAILPPEFDDFLLTRIAQSRRS
                     ADGHQAA"
BASE COUNT          262 a          544 c          549 g          286 t
ORIGIN      
        1 tgccgaggcg gttcttcacc gtctcctcct tgtcgtccac gcgctggatg agcgcctctc
       61 ccgtgatgtc gtccttcatg tcgttcttcg gcgggttgta cttcacgtga taggagcgcc
      121 ccgagggctg gtgcacgcgc cgccccgaca tgcgctcgat gatcaccgcg tcggggaccg
      181 cgatctccac cacgtgttcg aggcgcacgc cggaatcgcg catcgcctgc gcctgcggga
      241 tcgtgcgggg aaagccgtcg aagagaaacc cgctggcgca gtcgggcgcc ttgatgcgct
      301 ccttcaccag cccgatgatg atgtcgtcgg agacgaggaa gccgccatcc atcaccttct
      361 tggccgcgag cccgaggggc gtccccgcct tcacggcggc gcgcagcatg tcgcccgtcg
      421 agatctgcgg aatgcggtac ttctgcgtga tgaaggtcgc ctgggtgccc ttgccggctc
      481 ccggtccacc cagcaagatc aggcgcatcc gcaaccctcc gagggtcgtt tcttgtgaac
      541 ccgtgattat acgtgcccca gcttggcgcg gaccctctcc aggtcttccc gggtgtcgac
      601 tcccgcctcc atcggctcgt tccagaaggc gacggcgatc cggtgcccgt ggcccagcgc
      661 gcgcagctgc tcgagggcct cgaagcgctc cgccaccgtg ggcgaaagcg aggaagattc
      721 gcgcaggaac cgcacgcgag atgcgtagag gccgatgtgg cgataagccg cgaagccttc
      781 gggaaggatg tcgcgagtct tcgcgaacgc gtcgcgggcg tagggaatcg gcgcccgcga
      841 gaaatactgc gcgtacccct cggtgtcgat cacgaccttc acgacgttcg gatcgaagaa
      901 ggcctgcgcc gacgtgatcg gatgaacggc ggtcgagatc gacgcgttgg gacgcagcgc
      961 gagctcgctc gctaccttgc ggatcagggc gggatcgatg agcggctcgt cgccctggag
     1021 gttgaccacg atctcgtcgg gcttcaggcc gagcagcgcg accgcttcgg cgaggcgatc
     1081 ggtgcccgtc tcgtgcgacg tggaggtcgt gcacaccttg tggccgtgag cggcgaccgc
     1141 gtcgccaatg tcgctgtggt cggtcgcgac cacgacttcg tcggcgccgc tcgccagcgc
     1201 gcgctccacc acccacacca cgaggggctt gccggcgatg tcggcgagca tcttgcccgg
     1261 aaggcgcgtc gagcgcatgc gcgccgggat cacgaccttg aaggcgagcc gctccacggg
     1321 cgcttcaggg ctcttcttcg ggacgaagcg tgcgcgcttc ctcctcgagc atcaccggga
     1381 tgtcgtcctt cacggggaac gcgaggcggt cgcccttgca gatgagctcg gaagcctcct
     1441 tgcgccacag caagggcccc ttgcacagcg gacagacgag aatctcaagc agcttggtgt
     1501 ccatcggcgc tccggcggga ctgggcgatg cgggtgagaa ggaaatcatc gaactcgggc
     1561 ggcaggatcg catcgacccg caggtaccac atgcgtgaat cggcgaatgc cgcgcatttt
     1621 acggcatcct tctccgtcat g
//
LOCUS       DASVII010000311         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_517675,
            whole genome shotgun sequence.
ACCESSION   DASVII010000311 DASVII010000000
VERSION     DASVII010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517675"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1692)
                     /locus_tag="VF309_04070"
     CDS             complement(<1..>1692)
                     /locus_tag="VF309_04070"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX7465795.1"
                     /translation="DGAKPDSPSADSPPSWTIALGKLTLDGYRVTLHDATVKPALAEH
                     VNVAHLEASDLSSERGARSAMGAHIALDKSGSVDLEGAFALSPLELEAKIDARHVDIV
                     PMRRYVEQFKTVALKSGFASAKGALTIRGEGDAMRIGYAGAASVSNLATVDTTINEDL
                     LNWEAVRMTGIVLQYAAKDPLDVAIAEIGVQKAYARVVVNPDGKLNLQQLMAATPVEP
                     NAPAPAPAPGDAKPRSVRIDRITFSDSRLDFTDHFIKPNYNADVGGLAGTVTSLSSDP
                     ASRGVVDLKGSYDQVSPVTIAGTVNPLSGDLFLDIAAKGKDIELPKLSTYSLRYAGYG
                     ITKGRLTLDVRYHIEHGKLEGRNNIFLDQLTFGDHVDGPDVTKLPVLFAVNLLKNSKG
                     EINLELPVSGSLDDPQFAIGALISQVVSNLFKKALTAPFSLLTAAFGGGGSATSGEDL
                     AFVEFEPGREEIGVAGQKKLDAVAKALLDRPAIHLEMAASWDAQKDAQALKRSALLLR
                     VKEAKRTALGKSAPPLDAITFGDGEYARYLKIVYEREAPPKPASRDDATKAKEAAP"
BASE COUNT          255 a          540 c          585 g          312 t
ORIGIN      
        1 cggcgcggct tccttcgcct tcgtcgcgtc gtcccgggat gccggcttgg gcggcgcttc
       61 ccgctcgtag acgatcttga ggtagcgcgc gtattcgccg tcgccgaacg tgatcgcgtc
      121 caacgggggc gcggacttgc cgagcgcggt gcgcttggcc tccttcacgc gaagcaggag
      181 ggccgagcgc ttcagcgcct gcgcgtcctt ctgcgcgtcc cagctcgccg ccatctccag
      241 gtggatcgcg ggacgatcga ggagcgcctt cgccaccgcg tcgagcttct tctgtcccgc
      301 gacgccgatc tcttcgcgcc ccggctcgaa ttcgacgaag gcaaggtctt cgccacttgt
      361 cgcgctgccg ccgccgccga acgcggcggt gaggagcgag aagggcgcgg tcagcgcttt
      421 cttgaaaagg ttcgagacca cctgggagat gagcgcgccg atcgcgaact gtggatcatc
      481 gagggagccg ctgaccggaa gctcgaggtt gatctcgccc ttcgagttct tgaggaggtt
      541 cacggcgaac agcaccggaa gcttggtcac gtcgggcccg tccacgtgat cgccgaacgt
      601 gagctggtcg aggaagatgt tgttgcggcc ctcgagcttg ccgtgctcga tgtggtacct
      661 cacgtcgagc gtgagcctgc ccttggtgat gccgtagccg gcgtaacgca gcgaatacgt
      721 ggagagcttc ggcagctcga tgtccttgcc cttcgccgcg atgtcgagga agagatcgcc
      781 cgagagagga ttcacggtgc ccgcgatcgt cacgggggag acctggtcgt agctgccttt
      841 caaatcgacc acgccgcggc tcgcgggatc cgaggatagg ctcgtcacgg tccccgcaag
      901 gccgcccaca tccgcgttgt agttcggctt gatgaagtga tcggtgaaat cgagacggct
      961 gtcgctgaag gtgatgcgat cgatgcgcac gctgcggggc ttcgcgtcgc cgggcgccgg
     1021 cgcgggcgcg ggcgcattcg gctcgacggg ggtggcggcc atcagctgct gcaggttgag
     1081 cttgccgtcg ggattgacga ccacgcgcgc gtacgccttt tggacgccga tctcggcgat
     1141 ggcgacgtcg agcggatcct tcgccgcgta ctgcagcacg atgcccgtca tgcgaaccgc
     1201 ctcccagttg aggaggtcct cgttgatcgt cgtgtcgacg gtggcgagat tggaaacgct
     1261 cgcggcgccg gcatagccga tccgcatcgc atcgccttca ccgcgaatcg tgagcgcgcc
     1321 cttcgccgag gcgaatccgc tcttcaacgc gaccgtcttg aattgctcga cgtagcggcg
     1381 catgggaacg atgtccacgt ggcgcgcatc gatcttcgct tcgagctcca ggggcgagag
     1441 ggcgaacgca ccttcgagat cgacgctgcc ggacttgtcg agcgcgatat gcgcccccat
     1501 cgcgctgcgg gcgcccctct ccgacgagag atcgctcgcc tcgaggtgcg ccacgttcac
     1561 gtgctccgcc agggcgggct tcaccgtcgc gtcatgaagc gtgacgcggt agccgtcgag
     1621 cgtgagcttg ccgagggcga tggtccatga aggcggactg tcggcggacg ggctatcggg
     1681 cttggcgcca tc
//
LOCUS       DASVII010000312         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_445859,
            whole genome shotgun sequence.
ACCESSION   DASVII010000312 DASVII010000000
VERSION     DASVII010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445859"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /gene="malQ"
                     /locus_tag="VF309_04075"
     CDS             <1..1050
                     /gene="malQ"
                     /locus_tag="VF309_04075"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7465796.1"
                     /translation="SETEVDFHRWLQFELDRQLCRAATVARESGLGIGIYQDLAIGAA
                     PDSADVWAFGHLFLSGVSVGAPPDGYAPQGQNWGLPPIDPRQLVEDRYAFWIALIRAS
                     LRHAGALRIDHVLGLFRQFWIPAGMPGALGAYVRFPTEDMMGILALESVRHHALIVGE
                     DLGTVPPEVPGVLERWGILSSRVMYFERESDGRFRPASSYPKLSLTTADTHDMATITG
                     FWTARDIELQHMAGLIPSNEQAAAALAHRDNERRFLVERLVAEGALKSSLAESPSNSE
                     LRGGVHRFLCSTPAALVGVYLEDLVGEREPVNLPGVSPDLFRSWTRRLSRSLEGIADD
                     PEVATAFPDPVRAGR"
     gene            1070..>1722
                     /locus_tag="VF309_04080"
     CDS             1070..>1722
                     /locus_tag="VF309_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7465797.1"
                     /translation="MTIPIRATYRLQLSAAFTLDDARAIVPYLAQLGISHIYASPVLR
                     SRPGSTHGYDVVDPTRLDPELGSDASWDALILELRVHDMGLVLDIVPNHMGVGADNPF
                     WDDLFANGPASRYAGWFDIDWNAPARGLKGRVLIPILGAELAVAIEQRDFAIALDDGR
                     LRLHYFEHSFPLDPTTIPVVLELAIDDVPANVKMEDAAELAAIREALAALPPRLTSRP
                     "
BASE COUNT          250 a          647 c          526 g          299 t
ORIGIN      
        1 agcgagaccg aggtcgactt ccatcgctgg ctccagttcg agctcgatcg gcagctctgc
       61 cgcgccgcca cagttgcgcg tgagtccgga ctcggcatcg gcatctatca ggatctcgcg
      121 attggtgccg cgcccgacag cgcggacgtc tgggcgttcg gccatctctt tctgagcggc
      181 gtctccgtcg gcgcgccgcc cgacggctac gcgccgcagg gacagaactg gggactcccg
      241 cccatcgatc ctcgccagct cgtcgaggat cgatacgcat tctggatcgc gctcatccgc
      301 gcctcgctcc gccacgccgg cgcgctccgc atcgatcacg ttctcggcct cttccgccag
      361 ttctggatcc ccgccggcat gcctggcgcg ctcggcgcct acgtgcgctt tcccaccgaa
      421 gacatgatgg gcatcctcgc gctcgagagc gtgcgccatc acgcgctcat tgtcggcgag
      481 gatctcggca ccgtgccacc ggaagtgccg ggcgtgctcg agcgctgggg cattctctcg
      541 tcgcgcgtga tgtacttcga gcgcgagagc gacggccgct ttcgcccggc gtcgtcgtac
      601 cccaaactct ccctcaccac cgccgacacg cacgacatgg cgacgatcac cggcttctgg
      661 accgcgcgcg acatcgagct gcagcacatg gccggcctca tcccatcgaa cgaacaggcc
      721 gcggcagcgc tcgcgcatcg cgataacgag cgccgctttc tcgtcgagcg actggtggcc
      781 gagggcgcgc tcaagagcag cctcgcggag tcgccgagca actcggagct gcgcggcggc
      841 gtgcaccgct ttctctgctc gactcccgcg gcgctcgtcg gtgtctacct cgaggatctc
      901 gttggcgagc gcgagcccgt gaatctcccc ggtgtgagcc ccgatctctt tcgcagctgg
      961 acgcgccgcc tctcgcgttc gctcgagggc atcgccgatg accccgaggt cgcgaccgca
     1021 ttcccagatc ccgttcgcgc cggtcgctga cgcgagcgca gatgctccgg tgacgatccc
     1081 gatccgcgcg acctatcgcc tgcagctcag cgccgcgttc acgctcgacg atgcgagggc
     1141 gatcgtcccc tatctcgcgc agctcggcat ctcgcacatc tacgcgtcgc ccgtgcttcg
     1201 ctcgcgcccc ggaagcacgc acggttacga tgtcgtcgac cccacgcgcc tcgatcccga
     1261 gctcggcagc gacgcgagct gggacgcact gatcctcgag ctccgcgtgc acgacatggg
     1321 cctggtgctc gacatcgtgc ccaaccacat gggcgtcggc gccgacaatc cattctggga
     1381 cgatctcttc gcgaacggtc ccgcgtcgcg ctacgcgggc tggttcgaca tcgactggaa
     1441 cgcccccgcg cgcggcctca aaggtcgcgt cctcatcccc atactgggcg ccgagctcgc
     1501 ggtggcgata gaacagcgcg acttcgcgat cgcgttggat gacggccgcc ttcgactcca
     1561 ctatttcgag cactccttcc cgctcgaccc gacgacgatt cccgtagtgc tcgagctcgc
     1621 gatcgacgac gtgcccgcaa atgtgaagat ggaagatgcg gcggagctcg cggcgattcg
     1681 cgaggcgctc gccgcccttc ccccgcgcct cacttcgcgc cc
//
LOCUS       DASVII010000313         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_86196, whole
            genome shotgun sequence.
ACCESSION   DASVII010000313 DASVII010000000
VERSION     DASVII010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86196"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VF309_04085"
     CDS             <1..1048
                     /locus_tag="VF309_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7465798.1"
                     /translation="LRRLLGTSGAELFLYTANGHGVWEAVSENLLAPGEAALIPATGH
                     FSEQWSLLLEATGRRAIRTPYRPGYPIDPSTIEEALRNDAKREIAAVFVVHTDTASGI
                     THDVAAIRAAMDSTGHPALLVVDVVASLAAAPFAMDALGANVAVGASQKGLMSPPGLG
                     FVAVDARAAAVAARNTTPRYYWDWERRRGTQSYQKFCGTPPQNLLYGIEAALALIESE
                     GLDAIVARHASLARAVQAAVEGWATEGAVSLHCRVPEARSTSVTTVEVRAGIDVDALR
                     TVARERFQVAFAGGLGPFEGRAFRIGHLGDLNAAMILGCLAGVEAAMLAQGIPFGRDG
                     VARAVASLAKDLGR"
     gene            complement(1060..1704)
                     /locus_tag="VF309_04090"
     CDS             complement(1060..1704)
                     /locus_tag="VF309_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX7465799.1"
                     /translation="MERQRLDIAFVSDVACPWCAIGLASLDQALARLGGEGEAQVHVE
                     PFELNPDMGPEGAEVVPYLARKYGRTPEQVAQTQAMIRERGAEVGFSFGPRKHVWNTF
                     NAHRVLHWAGLEGRALELKRALLRAYHGEGRNPGATDVLVELAGTVGLDAARAKSIVQ
                     SDEFAPEVRARERYWLQRGVNGVPHVVVNDSYAIEGAQPPEYFEQALRQIARSR"
BASE COUNT          275 a          634 c          598 g          312 t
ORIGIN      
        1 cctgaggcgg ctgctcggga cttccggcgc cgagcttttc ctctatacgg cgaatggcca
       61 cggcgtttgg gaggcggtga gcgagaacct gctggctccc ggcgaggcgg cactcattcc
      121 ggcgaccggg cacttttccg agcaatggtc attgctgctc gaggcgacgg gccggcgcgc
      181 gatccgcacg ccgtatcgcc ccggctaccc gatcgaccca tcgacgatcg aggaggctct
      241 tcgcaacgat gcgaagcgcg agatcgccgc ggtcttcgtc gtgcataccg ataccgcgag
      301 cggcatcacg cacgacgtcg ccgcgattcg ggctgccatg gactcgacgg ggcaccccgc
      361 cctcctcgtg gtcgatgtcg tcgcatcgct cgccgccgcg ccattcgcga tggatgcgct
      421 tggcgccaat gtcgcggtcg gcgcttcgca gaagggactc atgtcgcccc cgggcctggg
      481 cttcgtcgcc gtggacgcgc gcgccgccgc cgtcgccgcg cgcaacacca ctccgcgcta
      541 ttactgggac tgggagcgcc gccgcgggac gcagagctac cagaagttct gcggcacgcc
      601 gccgcagaac ctgctctacg gcatcgaggc cgccttggcg ctcatcgaga gtgaagggct
      661 tgatgcgatc gtcgcgcgcc atgcgtcgct tgcacgcgcg gtgcaggcgg cggtggaagg
      721 ctgggcgacc gaaggcgctg tctccctgca ctgtcgcgtt cccgaagccc gatcgacctc
      781 cgtgacgacc gtcgaggtgc gcgcaggtat cgacgtggat gcgctccgga ccgtcgcacg
      841 tgagcgcttt caagtcgcct tcgcgggagg attgggcccg ttcgaaggac gagctttccg
      901 catcggccac ttgggtgacc tcaatgccgc gatgatcctc ggctgccttg ccggcgtcga
      961 agcggccatg ctggcgcaag gcatcccctt tggccgcgat ggcgtcgctc gcgcggttgc
     1021 cagcctcgcc aaggatttgg gccgctgact tctggagacc tagcgcgagc gcgcgatctg
     1081 ccggagcgcc tgctcgaagt actccggtgg ctgggcccct tcgatcgcgt acgaatcgtt
     1141 cacgaccacg tgcggcacgc cgttgacgcc gcgttgcagc cagtagcgtt cgcgcgcacg
     1201 gacctcgggg gcgaattcgt cgctttgcac gatcgacttc gcgcgcgccg catccaggcc
     1261 aacagtgccg gcgagctcca cgagcacgtc cgtcgcgccc gggttgcggc cttcaccgtg
     1321 ataggcgcga agcagcgctc gcttgagctc aagggcacgc ccctcgagcc cggcccaatg
     1381 cagcacacgg tgcgcgttga aggtgttcca cacgtgcttg cgaggcccga acgaaaagcc
     1441 aacctctgcc ccgcgctcgc gaatcatcgc ctgcgtctgg gctacctgct ccggcgtgcg
     1501 gccgtacttg cgagcgaggt acggcacgac ttccgcgcct tcgggaccca tgtccggatt
     1561 gagctcgaag ggctcgacat gcacctgcgc ttccccctcg ccgcccaggc gcgcgagtgc
     1621 ctggtcgagc gaggcaagcc cgatcgcgca ccaggggcag gccacatcgg agacgaaggc
     1681 gatgtcgaga cgttgtcgtt ccattttatc cttgcctcgc tagagacgaa cttccatcgg
     1741 accgtacttc ttcggatcat cgaacgagag cacgcgcctt gccggatccc acgcaatcct
     1801 ggcgccggcg agcgcctca
//
LOCUS       DASVII010000314         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_554067,
            whole genome shotgun sequence.
ACCESSION   DASVII010000314 DASVII010000000
VERSION     DASVII010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554067"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1126
                     /locus_tag="VF309_04095"
     CDS             131..1126
                     /locus_tag="VF309_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEX7465800.1"
                     /translation="MFWKTAIALLITVAAATLTKSAIVTVVTFLLTMIIVVIAEGVRI
                     VPQQNAWVIERLGKFFRVLDPGLQIIVPFVDRVAYKHSLKEYPLDVAEQVCITKDNTQ
                     LAVDGIIYFQVVDPRLASYGSSNYIQAITQLAQTALRSVIGKMELDKTFESRDEINHT
                     IVGVLDEAGRTWGIKVLRYEIKSLTPPEAILRSMQAQITAEREKRAVIATSEGKRQQE
                     INVADGSRQAAILASEGQKTAAINKAQGEAAAIQLVADATANAVRAVGSAISTPGGTS
                     AANLKVSELYIEAFKELARTNNTLIIPSNLSDIAGLVASAMTVLDRPKMTEAVVK"
     gene            1280..1954
                     /locus_tag="VF309_04100"
     CDS             1280..1954
                     /locus_tag="VF309_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX7465801.1"
                     /translation="MKKSSSSKQWLRRHVNDPYVQRSKKEGYRSRSAYKLTEIDDRDR
                     LLKAGMVVVDLGSAPGGWSQVAAKRAGPTATVIAMDLLPMEPVAGVTFLQGDFTSRAG
                     SAALRHALDGRKADVVLADMAPNMTGIAVSDQARTMELAELARDFALLHLQREGALLV
                     KIFQGAGYDEYLKMLRRAFVKVVVRKPDASRDESAEQYLLARGLRETDAPPQPGVDIG
                     RKSPIC"
     gene            2031..>2410
                     /locus_tag="VF309_04105"
     CDS             2031..>2410
                     /locus_tag="VF309_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX7465802.1"
                     /translation="MNNTIRTIMVWLVIGVILTMVFMQVGNRTSSQNSLAYSTFMEEA
                     RQGHIASVKLDNNRTLKATSRDGKTYTVNTPGIQDPWMVGDLMKFGVAVEAQPVEEQS
                     LLMSIFVSWFPMLLLIGVWIFFMR"
BASE COUNT          478 a          795 c          742 g          395 t
ORIGIN      
        1 cgtgcgcccg gacgatcgcc ccgtgcccgg caccacgctc tacatcgaag cccaggaagg
       61 caacacgctc gtcgtcgcgg ccgcaccacc cgcgcgctag aaaaccggca ccaagaaaag
      121 ggagatcaaa atgttctgga aaaccgccat cgccttactg ataaccgtcg ccgccgcgac
      181 gctcaccaag agcgcgatcg tgacggtcgt cacgttcctc ctcacgatga tcatcgtcgt
      241 gatcgccgag ggcgtgcgca tcgtcccgca gcagaacgcc tgggtcatcg agcgactcgg
      301 caagttcttt cgcgtgctcg atccgggcct gcagatcatc gtgcccttcg tcgaccgcgt
      361 ggcctacaag cactcgctca aggaatatcc gctcgatgtc gccgagcagg tgtgcatcac
      421 caaggacaac acgcagctcg cggtcgacgg gatcatttat ttccaggtgg tcgacccccg
      481 gctcgcctcg tatggctcgt cgaactacat ccaggcgatc acgcagctcg cgcagacggc
      541 gctgcgctcg gtgatcggca agatggagct cgacaagacc ttcgagagca gggacgagat
      601 caaccacacg atcgtcggcg tcctggacga ggccggccgc acgtggggca tcaaggtgct
      661 gcgctacgag atcaagagcc tcacgccgcc ggaagcgatc ctgcgctcca tgcaggcgca
      721 gatcaccgcc gagcgcgaga agcgcgcggt gatcgcgact tccgaaggca agcgccagca
      781 ggagatcaac gtggccgacg gctcgcgcca ggcggcgatc ctcgcctccg aaggccagaa
      841 gaccgccgcc atcaacaagg cgcagggcga ggccgccgcg atccagctcg tcgccgacgc
      901 gaccgccaac gcggtgcgcg cggtgggctc ggcgatctcc acgcccggag gcacgagcgc
      961 ggcgaacctg aaggtctcgg agctctacat cgaagccttc aaggagctcg cgaggacgaa
     1021 caacacgctc atcatcccga gcaacctctc cgacatcgcg ggcctcgtcg cgagcgcgat
     1081 gaccgtcctc gaccgcccga agatgaccga agccgtggtc aaatagataa gaggctttga
     1141 atccgcagat aaacgcagat gaacgcggta accccaaggc cgagacgtgc ttcgtgacgt
     1201 tggatccatc agctcgaatg aacacaggtt ttgcctttgc cgcgttcatc tgcgtttatc
     1261 tgcggatccc ggggttcgct tgaaaaaatc ctcttcgagc aagcagtggc tgcgcaggca
     1321 cgtgaatgat ccgtacgtgc agcgctcgaa gaaggagggc tatcgctcgc gctcggccta
     1381 caagctcacc gagatcgacg accgcgaccg gctcctgaag gccggcatgg tcgtcgtgga
     1441 cctgggctcg gcgccggggg gctggtcgca ggtcgcggcg aagcgcgccg ggcccacggc
     1501 gaccgtgatc gcgatggacc tgctccccat ggagccggtc gcaggcgtga ccttcctgca
     1561 gggcgacttc acctcccgcg cgggaagcgc ggcacttcgc catgcgctgg acgggcgcaa
     1621 ggccgacgtc gtcctcgccg acatggcccc caacatgacg gggatcgcgg taagcgacca
     1681 ggcgcgcacc atggagcttg ccgagctcgc gcgcgatttc gcgctcctgc acctgcaacg
     1741 cgagggcgcg ctgctggtca agatcttcca gggcgcgggg tatgatgaat acctcaaaat
     1801 gctgcgccgg gccttcgtga aagtggtggt gcgcaagccc gatgcgtctc gcgacgagag
     1861 cgccgagcag taccttctcg cgcgcggtct cagggaaacc gacgctcccc cgcagccagg
     1921 ggttgatatc ggtcgaaaga gcccgatctg ttagactagc ttcgcctcca tatccctgaa
     1981 attcaagggt ttttcccccg gcaaggattt tcacgctgaa ggatccgctc gtgaacaaca
     2041 ctattcgcac catcatggtc tggctcgtca tcggcgtgat actgaccatg gtgttcatgc
     2101 aggtcggcaa ccgcacctcg agccagaact cgctcgccta ttcgaccttc atggaggaag
     2161 cgcgccaggg gcacatcgcc agcgtgaagc tcgacaacaa ccgcacgctc aaggccactt
     2221 cgcgcgacgg caagacctac acagtgaaca cccccggcat ccaggacccg tggatggtcg
     2281 gcgacctcat gaagttcggc gtcgccgtgg aggcgcagcc ggtcgaggag cagtcgctgc
     2341 tcatgagcat cttcgtgtcg tggttcccga tgctgctgct catcggcgtg tggatcttct
     2401 tcatgcgcca
//
LOCUS       DASVII010000315         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_410162,
            whole genome shotgun sequence.
ACCESSION   DASVII010000315 DASVII010000000
VERSION     DASVII010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410162"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..959)
                     /locus_tag="VF309_04110"
     CDS             complement(159..959)
                     /locus_tag="VF309_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7465803.1"
                     /translation="MNRSVTALAALLLACALAGCSFFASKLDPKKDWQAADYYKAAKE
                     QFDAQNWDAAIKVYDELEAKFPFGRFAQQAQIEIAYAYYKQGEIAQSVTALEKFVKLH
                     PNHPNVDYALYLKALCNFKEDLGPFSRLVTQDLADRDPKAARESFEGFKDLVTRFPES
                     RYAADSRERMAYLVEALARHEVRVARYYLSRGAYLAAANRAQDAIVRFPNSPTHRESL
                     DIMVEAYDRMGMKELRDDSKRVLVKNFPEDPLGQSGKNRGGSWWKFWQ"
     gene            958..1908
                     /locus_tag="VF309_04115"
     CDS             958..1908
                     /locus_tag="VF309_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7465804.1"
                     /translation="MVNPVSRSTNYSATEVLSRELKVTIPAGMAGLRLDQALAKLLPQ
                     ESRTRLARLIEEGHVRVDGEQVPAKRKLKSGEVVEVALAPRVEESAYGPEAIALEIVH
                     EDDDVLVVNKPAGLVVHPGSGNWAGTMLNALLHHAPALEHLARAGIVHRLDKDTSGLL
                     VVAKNEPAQLALVRQLQARTVKRTYLALARGRIATPGKVDAPIGRHPVHRTRMAIVAG
                     GKAAITHYRVRESFPSHTLLECDLETGRTHQIRVHLASIGHPLEGDPVYAKRSDGLSF
                     ARQALHAWKLAFVHPRSGAPQSFEAPLPEDFRALLESLRR"
     gene            1905..>2063
                     /locus_tag="VF309_04120"
     CDS             1905..>2063
                     /locus_tag="VF309_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEX7465805.1"
                     /translation="MIPREWITPDWPAPARVRAFVTTRHGGVSAGEYASLNLGTRGGD
                     DPRDVASNR"
BASE COUNT          333 a          687 c          704 g          339 t
ORIGIN      
        1 agaagagctc aaggaaattc gagcgctatc gtaagtagcg agctctctcc ccttcgaaag
       61 gtcgaggtcg ccgggtcgag cttcaccccc acttcaatga ggcgatagcc gaggggtaac
      121 tccggattcc cgcctccgcg agaatgacga cgctccggct actgccagaa cttccaccac
      181 gagccgccgc ggttcttgcc gctctgcccc agcggatcct cggggaagtt cttcacgagg
      241 acgcgcttgg agtcgtcgcg aagctccttc atccccatgc ggtcgtaggc ctcgaccatg
      301 atgtcgagcg attcgcggtg ggtgggggag ttggggaagc gcacgatcgc gtcctgcgcg
      361 cggttggcgg cggcgagata ggcgccgcgc gagaggtagt agcgcgcgac gcgcacctcg
      421 tggcgcgcga gggcttccac gagataggcc atgcgctcgc gggaatccgc cgcgtagcgg
      481 ctttcgggaa agcgcgtgac gaggtccttg aagccttcga aggactcgcg cgcggccttg
      541 gggtcgcggt cggcgaggtc ctgggtgacg agccgcgaga aaggcccgag gtcttccttg
      601 aagttgcaga gggccttgag atagagcgcg tagtcgacgt tcgggtggtt cgggtgcagc
      661 ttcacgaact tctcgagcgc ggtgaccgac tgggcgatct cgccctgctt gtagtacgcg
      721 taggcgatct cgatctgcgc ctgctgcgcg aagcgcccga acgggaactt cgcctcgagc
      781 tcgtcgtaga ccttgatcgc cgcgtcccag ttctgcgcgt cgaactgctc cttcgccgcc
      841 ttgtagtagt ccgccgcctg ccaatccttc ttgggatcga gcttcgaggc gaaaaagctg
      901 catccggcga gcgcgcacgc gaggaggagg gctgccaatg ccgttacact tcgattcatg
      961 gtgaatcccg tttcgcgaag cacgaattat agcgcgaccg aggtcctttc ccgcgagctg
     1021 aaagtcacga ttccggccgg gatggcggga ctgcgtctcg accaggctct cgcgaagctc
     1081 cttccgcagg agtcgcgcac gcggctcgcg cggctcatcg aggagggcca cgtgcgcgtc
     1141 gacggcgagc aggtcccggc gaagcgcaag ctgaagagcg gcgaggtggt cgaggtcgcg
     1201 ctcgcgccgc gcgtcgagga atcggcctat ggccccgagg cgatcgccct cgagatcgtc
     1261 cacgaggacg acgacgtgct cgtcgtgaac aagcccgcgg gactcgtggt gcatccggga
     1321 agcggcaact gggcggggac gatgctgaac gcgctccttc accacgcgcc ggcgctggag
     1381 catctcgcgc gcgcggggat cgtgcaccgc ctcgacaagg acacgagcgg ccttctcgtg
     1441 gtcgcgaaga acgagcccgc gcagctcgcg ctcgtgcgcc agctccaggc gcggaccgtg
     1501 aagcgcacgt atctcgccct cgcgcgcgga aggatcgcca cgccggggaa ggtcgacgcg
     1561 cccatcggcc ggcatcccgt ccaccgcacg cgcatggcga tcgtcgccgg cggcaaggcc
     1621 gcgatcactc attaccgcgt gcgcgaatcg tttccgtcgc acacgctcct cgaatgcgac
     1681 ctcgagaccg ggcgcaccca ccagattcgc gtccatctcg cttccatcgg ccatcccctc
     1741 gagggcgatc cggtctatgc gaagcgcagc gacggccttt cgttcgcgcg gcaggcgctg
     1801 cacgcgtgga agctcgcctt cgtccacccg cgcagcggcg cgccgcaatc gttcgaggcg
     1861 ccgctgcccg aggattttcg cgcgctcctc gagagcctgc ggcgatgatt ccgcgcgagt
     1921 ggatcacgcc cgattggccc gcgcccgccc gcgtgcgcgc gttcgtcacg acgcgccatg
     1981 gcggcgtgag cgcgggtgag tacgcctcgt tgaacctcgg cacgcgcggc ggagacgatc
     2041 cgcgcgacgt cgcgagcaac cgc
//
LOCUS       DASVII010000316         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_590122,
            whole genome shotgun sequence.
ACCESSION   DASVII010000316 DASVII010000000
VERSION     DASVII010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590122"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF309_04125"
     CDS             complement(<1..204)
                     /locus_tag="VF309_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465806.1"
                     /translation="MSGLALTRREFLSGGGALVVSFSLAPRAAAQAGAGAKPAAKLPA
                     SLDRFPMLDSWIGIGADGRVTVFT"
     gene            complement(201..668)
                     /locus_tag="VF309_04130"
     CDS             complement(201..668)
                     /locus_tag="VF309_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7465807.1"
                     /translation="MIAIEVNGKVHEVDADPETPLLYVLRNDLALNGAKFGCGLGQCG
                     ACTVLVDGKAVFSCMTPISTLGGRRIRTVEGLGTIERPGPLQRAFIEEQAAQCGYCIP
                     GMMMRAQALLESNPAPSDADIRKALEPNLCRCGTHMRILRAVGRAAQLMKVKA"
     gene            complement(665..1930)
                     /locus_tag="VF309_04135"
     CDS             complement(665..1930)
                     /locus_tag="VF309_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7465808.1"
                     /translation="MAQLRLVMRIPAVVAAALAVSCALPPPSPLGEPGPRAFDRARIQ
                     KGAQLAAIGNCVSCHTAKGGRAFAGGYALKTPFGTVHGSNITPDPETGIGRWSEADFT
                     RALREGVSPEGHHYYPAFPYDYFTRLTDDDVGSLYAFVMTREPVRNTPPANTMIVPRF
                     AVAAWKQRYFDRAPFKPDPARGERWNRGAYLAQALAHCSACHTPRNALGAEERARFMS
                     GGEADGWHAPALNEHSSSPVAWNEESLTAYLLTGLVDAHAITAGPMEGVIDNLSRASR
                     DDVAALGTYIASLEVRDKDTLFRQSQAALLAAAKPASRGAGRGAAVYAGACGDCHDRG
                     REAEGGALQLPLAIALSLPTPANLIHVVREGIAPKPHEARPWMPEFAGALTDGEVVDL
                     VVYLRALSGKPPWTDVPAEVRKISQGESQ"
     gene            2066..2323
                     /locus_tag="VF309_04140"
     CDS             2066..2323
                     /locus_tag="VF309_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7465809.1"
                     /translation="MSHLQIGHSREIPTPLQAPSDTPSITVTVGTPLSAAARELILST
                     LQFCGGERKRTAEMLGICTKTLYNRLREYGIRVEQNQPGEL"
BASE COUNT          386 a          810 c          834 g          371 t
ORIGIN      
        1 ggtgaagacg gtgacgcggc catcggcgcc gatgccgatc cacgagtcga gcatcgggaa
       61 tcggtcgagg ctggccggga gtttcgcggc gggcttcgca cccgcaccgg cctgcgccgc
      121 ggcgcgcggc gcgagcgaga agctcaccac cagggcgccg ccgccggaaa ggaattcgcg
      181 cctcgtgagc gcgagcccgc tcatgccttc accttcatca gctgcgccgc gcgccccacg
      241 gcgcgcagga tgcgcatatg cgtgccgcac cggcaaaggt tcggctcgag cgccttgcga
      301 atgtcggcgt cgctcggcgc ggggttggat tcgaggagcg cctgcgcgcg catcatcatt
      361 cccgggatgc aatagccgca ttgcgccgcc tgctcctcga tgaaggcgcg ctgcaggggg
      421 ccgggccgct cgatcgtgcc cagcccttcg accgtgcgga tcctgcgccc gccgagagtc
      481 gagatcggcg tcatgcacga gaacaccgcc ttgccgtcga cgaggacggt gcaagcgccg
      541 cactggccga gcccgcatcc gaacttcgcg ccgttcagcg cgaggtcgtt gcgcagcacg
      601 taaaggagcg gcgtctccgg gtcggcgtcg acctcgtgca cctttccgtt cacctcgatc
      661 gcgatcattg cgactctccc tgggatatct tgcgtacctc ggcgggcacg tcggtccatg
      721 gcggcttgcc gctcagcgcg cgcagataga cgacgagatc gacgacctcg ccatcggtga
      781 gcgcgccggc gaactcgggc atccacggcc gcgcctcgtg gggcttcggc gcgatgcctt
      841 cgcgaacgac atggatgaga ttggccgggg tcggaagcga cagcgcgatc gcaagcggca
      901 gctgcagcgc cccgccttcg gcctcgcgcc cgcgatcgtg acaatcgccg cacgcgcccg
      961 cgtagaccgc agccccgcgg ccggctccac gcgacgcggg tttcgcagcc gcgagaagcg
     1021 ccgcctgcga ttgccggaag agcgtgtcct tgtcgcgcac ttccagcgaa gcgatgtagg
     1081 tgcccagggc ggccacgtcg tcgcgcgagg cacgggaaag attgtcgatg acgccttcca
     1141 tgggcccggc cgtgatcgcg tgggcgtcga cgaggccggt gagaaggtag gcggtaaggg
     1201 actcctcgtt ccaagccacc ggcgacgaag aatgctcgtt cagcgcgggg gcatgccagc
     1261 cgtccgcctc gccgccggac atgaaccgcg cgcgttcctc ggcgccgagc gcgttgcgcg
     1321 gcgtgtggca ggcgctgcag tgcgcgaggg cttgcgcaag gtaagcgccg cgattccagc
     1381 gctcaccccg cgccggatcg ggcttgaacg gcgcgcggtc gaagtatcgc tgcttccagg
     1441 ccgccaccgc gaagcgcggc acgatcatcg tgttggcggg cggcgtattg cgcaccggct
     1501 cgcgggtcat cacgaaggcg tagagcgagc cgacgtcatc gtccgtgagg cgcgtgaagt
     1561 agtcataggg aaacgcgggg tagtagtgat gcccctcggg cgacacgcct tcgcgcagcg
     1621 cgcgcgtgaa atcggcttcg ctccagcgcc cgatacccgt ttccggatcc ggcgtgatgt
     1681 tgctgccgtg caccgtgccg aaaggcgtct tcagcgcgta gccgcccgcg aaggccctgc
     1741 cgcccttcgc cgtgtggcac gagacgcagt tgccgatcgc ggcgagctgc gcgcccttct
     1801 ggatgcgcgc ccgatcgaac gcgcgaggcc cgggttcccc gaggggagat ggcggcggca
     1861 acgcgcacga aacggcgagc gccgcggcaa ccacggcggg aattcgcatc acgaggcgga
     1921 gctgtgccat ggaatgatta tgccagccgc tccgacatcg gattcacatc attaattccc
     1981 gagcaatgta ggacatggcc gacagttttc tgtgggcgcg tggttcaaca tgaaagtact
     2041 tcgcattggc aggagcgatt cacacatgtc gcatttgcag atcgggcatt cgcgcgagat
     2101 ccccacgccg ttgcaggcgc cttccgatac gccctcgatc accgtgacgg tcggcacgcc
     2161 gctctcggcc gcggcgcgcg agctcatcct ttcgacgctg caattttgcg gcggagagcg
     2221 caagcgcacc gccgaaatgc tcggcatctg caccaagacg ctctataacc gcctgcgtga
     2281 gtacggaatc cgcgtggagc agaaccagcc gggcgagctc tagggagcct agtccgaggt
     2341 gatcgtgggc gagccgcggc cggcgagtgc cgcggggccg tgcagctgcg ccaccgcatc
     2401 g
//
LOCUS       DASVII010000317         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_50879, whole
            genome shotgun sequence.
ACCESSION   DASVII010000317 DASVII010000000
VERSION     DASVII010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50879"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VF309_04145"
     CDS             complement(<1..696)
                     /locus_tag="VF309_04145"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX7465810.1"
                     /translation="MTTFLSRYHARLGVAPAAVALGLHCFAALAQAPSTAAPAAPPAA
                     SGPASGVTTSNGDEKITVNFVNADIQSVIKTVGQHTGRNFILDPRVTGTVNIVSDHPV
                     SKDMLYQILLSTLRVQGYAAVEDGGFVKIVPEAEAKTSAGPVGEQAARTPGDRIVTQV
                     FMLQNESAVQLVPVLRPLITANNFIAAYPNNNAIVITDYAENVRRIQRIIQAIDIPSS
                     GEVQVVRMQNASAI"
     gene            complement(790..1608)
                     /locus_tag="VF309_04150"
     CDS             complement(790..1608)
                     /locus_tag="VF309_04150"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX7465811.1"
                     /translation="MATLATDQRAAPGLFGNLVVAALVLLLAYQLAHWTWVFVAPAPV
                     AAVPDGAPAVNLAAVAKLFGAGPPANSPGAAASTSGLRLKGVIAPDQGPAASAIFSTG
                     AGKDLAVFVDREVQPGVKLLEVRPDYVIVSRAGERERIDLDTRRASVAATSTASRVTG
                     FRINVAKSGANNFSLSRKELDDALRDPQQLGYLGQIGMPPSGGVRMDAAPGGSLAQKL
                     GLQPGDIIKKLNGQNVASPGDLARLYQQFGTLSMIQAEVQRGSATIQLSYAIQP"
     gene            complement(1679..3430)
                     /locus_tag="VF309_04155"
     CDS             complement(1679..3430)
                     /locus_tag="VF309_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465812.1"
                     /translation="MEAADMRKVVRTASPSAESKLDPQAESDEMSGSINDQIFDSLLE
                     YDYLARPAKLKPRAAAALPEVSADGTTFTIRLKHGVYFTPDPAFRGARRELVAQDYVY
                     SIKRLLDPKLTSQWLFLVEGKIRGADALMAEAKKSQRFDYDRPVEGLAALDRYTLRIR
                     LNNTDFGFPYVLALPATAAVAREVREYYGDDFHAHPIGTGPYFLKEWVRGSRVVLEAN
                     PDFREETFESVGGDDAYSKEIAASLRGKRIPIVGRIEIYVIEEAQPRWLSFLNGEHDY
                     IRPLPEEFAQIAMPGGALAPNLRRKGLRTTPDEVAYTTYTTFNLQPEIDGRRNDVGGY
                     SPERVALRRAIAMAFRIDEQIAILDKHQSTRAHSPLHPAVAGYDPKFVSPTLEYNPAK
                     AKALLDMFGFVDRDGDGYRENPDGSPLSFEHASIPTVRERQRNELWKKSMDDIGVRVT
                     FGKVEKLPELRKQAQLGKVQSFSYGWIADYPDGENFLQLFWKASIGQTNYAMFDLPEY
                     NALYEKAKLLPDGPERNALYERMVKLILVYVPWMVETYKAQNIVMQPWLLNFKKHPFS
                     HEPWRYLDIDLSRHPPS"
BASE COUNT          565 a         1106 c         1243 g          636 t
ORIGIN      
        1 gatcgccgag gcgttctgca tgcgaacgac ctgcacctcg ccggaggagg gaatgtcgat
       61 cgcctggatg atgcgctgga tgcggcgcac gttctcggca tagtcggtga tgacgatcgc
      121 gttgttgttg ggataggccg cgatgaaatt gttcgcggtg atgagcgggc gcagcaccgg
      181 cacgagctgc accgcggact cgttctgcag catgaagacc tgcgtgacga tgcgatcgcc
      241 cggtgtgcgc gcggcctgct cgcctaccgg tcccgcgctc gtcttcgctt cggcttccgg
      301 aacgatcttc acgaagccgc cgtcttccac cgcggcgtag ccctgcacgc gcagcgtcga
      361 gaggaggatc tggtagagca tgtccttcga caccgggtga tccgagacga tgttcaccgt
      421 gccggtcacg cgcggatcga ggatgaagtt gcgcccggtg tgctggccga ccgtcttgat
      481 caccgactgg atgtcggcgt tgacgaagtt caccgtgatc ttctcgtcgc cattggaggt
      541 cgtgaccccc gaggcggggc cggaggcggc gggcggggcc gccggagcgg ccgtcgaggg
      601 cgcttgcgcg agggccgcga agcaatgcag tcccagcgcc acggcggcgg gggcgacgcc
      661 caggcgggcg tggtatcgac ttagaaacgt tgtcacgtag tggggcttct gctttgattt
      721 atctggcatt gtaagacgaa aaccggggca agaccccggc ttcgcgaccg actccacatt
      781 gaggagcgtc tagggctgga tcgcgtagga gagctggatc gtcgcgctgc cgcgctggac
      841 ttcggcctgg atcatcgaaa gcgtcccgaa ttgctggtaa aggcgcgcca ggtcgcccgg
      901 cgaggcgacg ttctgcccat tcaatttctt gatgatgtcg ccgggctgca gtcccagctt
      961 ctgcgcgagg ctgccgccgg gcgcggcgtc catgcgcacg cccccgctcg gaggcattcc
     1021 gatctggccg aggtagccca gctgttgggg atcgcgcagc gcgtcgtcga gctccttgcg
     1081 cgagaggctg aagttgttcg cgccggattt cgcgacgttg atcctgaatc ccgtcacccg
     1141 gctcgcggtg gaagtcgcgg cgacgctcgc gcggcgcgtg tcgagatcga tgcgctcgcg
     1201 ctcgccggcg cgcgagacga tcacgtagtc gggccgcacc tcgaggagct tcacgcccgg
     1261 ctgcacctcg cgatcgacga agaccgcgag gtccttgccg gctcccgtgg aaaagatcgc
     1321 cgaggccgca ggcccctggt cgggcgcgat cacgcccttc aaccgcagtc ccgaggtcga
     1381 ggcggcggcg cccggcgaat tcgcgggcgg gcccgcgccg aaaagcttcg ccaccgccgc
     1441 tagattcacc gccggcgcgc cgtcgggcac cgcggccacc ggcgcgggcg cgacgaatac
     1501 ccacgtccag tgcgcgagct ggtacgcgag gagcaggacg agcgcggcca cgacgaggtt
     1561 gccgaagagg ccgggggccg ccctttgatc cgttgccagt gtggccatcc ggaaattaga
     1621 tcatgaagcg ggctgtgagt gatgcggaca cgcggctttg aatccgcgga caaacgactt
     1681 agctcggcgg atggcgggag aggtcgatgt cgaggtagcg ccagggctcg tgcgagaagg
     1741 gatgcttctt gaagttgagg agccagggct gcatcacgat gttctgcgcc ttgtaggtct
     1801 ccaccatcca gggcacatag acgaggatca gcttcaccat gcgctcgtac aacgcgttgc
     1861 gctcggggcc gtcgggaagg agcttcgcct tctcgtagag cgcgttgtat tcgggcaggt
     1921 cgaacatcgc gtagttggtc tgcccgatgg aggctttcca gaagagctgg aggaagttct
     1981 cgccgtcggg atagtcggcg atccatccgt agctgaacga ctgcaccttg ccgagctgcg
     2041 cctgcttgcg aagctccggc agcttctcca ccttgccgaa ggtcacgcgc acgccgatgt
     2101 cgtccatcga tttcttccac agctcgttcc tctgccgctc gcgcaccgtg ggaatggagg
     2161 cgtgctcgaa cgaaaggggc gacccgtcgg gattctcgcg atagccgtcg ccgtcgcggt
     2221 cgacgaagcc gaacatgtcg aggagcgcct tcgccttcgc cgggttgtac tcgagggtcg
     2281 ggctcacgaa cttcgggtcg tatcccgcga ccgccgggtg cagcggcgag tgcgcgcggg
     2341 tcgactggtg cttgtcgagg atggcgatct gctcgtcgat gcgaaacgcc atggcgatcg
     2401 cgcgccgcag cgccacgcgc tcgggagagt agccgcccac gtcgttcctc ctgccgtcga
     2461 tctcgggctg caggttgaag gtcgtgtagg tcgtgtaggc gacctcgtcg ggcgtggtgc
     2521 gcagcccctt cctgcgcagg ttcggcgcga gcgccccgcc gggcatcgcg atctgcgcga
     2581 actcctcggg gagcgggcgg atgtagtcgt gctcgccgtt gaggaacgag agccagcgcg
     2641 gctgcgcctc ctcgatcacg tagatctcga tgcgcccgac gatcgggatt cgcttgccgc
     2701 gcaggctcgc ggcgatctcc ttcgagtacg cgtcgtcgcc gcccacgctc tcgaaggtct
     2761 cctcgcggaa atcgggattc gcctcgagca ccacgcgcga gccgcgcacc cactccttca
     2821 ggaagtaggg cccggtgccg atcgggtggg cgtggaaatc gtctccgtag tattcgcgca
     2881 cctcgcgggc gacggcggcc gtggccggca acgcgagcac gtaggggaat ccgaagtcgg
     2941 tattgttgag gcggatgcgc agcgtgtacc ggtcgagggc ggccagcccc tcgacgggcc
     3001 ggtcgtagtc gaagcgctgg ctcttcttcg cctccgccat cagcgcgtcg gcgcccctga
     3061 tctttccctc gacgaggaac agccactggc tggtgagctt cgggtcgagg aggcgcttga
     3121 tcgaatagac gtagtcctgc gcgacgagct cgcggcgcgc gccccggaac gcgggatcgg
     3181 gcgtgaagta gacgccatgc ttgaggcgga tggtgaacgt ggttccgtcg gcgctcacct
     3241 cgggaagcgc cgccgcggcg cggggcttca gcttcgcggg gcgcgctagg tagtcgtact
     3301 cgaggaggga atcgaagatc tggtcgttga tcgacccgct catctcgtcg ctctcggctt
     3361 gcgggtcgag cttgctctcc gcgctgggcg atgccgtcct caccaccttg cgcatgtcgg
     3421 ccgcttccac agccgtgatg agagacattg caaccaggca ggccaagagc tttgaatccg
     3481 cagataaacg cagataaacg cggttgaaag cgaagagcat cttgatgatc actcgcggaa
     3541 gtggaggcac
//
LOCUS       DASVII010000318         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_662166,
            whole genome shotgun sequence.
ACCESSION   DASVII010000318 DASVII010000000
VERSION     DASVII010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662166"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VF309_04160"
     CDS             complement(<1..429)
                     /locus_tag="VF309_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465813.1"
                     /translation="MRAVLAAGGALAFTQCAFAQDYPTPSFDCANVMSPQELRICRSQ
                     ELSELDGRHGSLVERAIRASSNRDETRAEMDAWLERVRNPCQTDSCLVEAYTSHIREL
                     ERIVPAVIAQPAFTAPARRDLAAAVAKAAPEPVKPAAVKTA"
     gene            complement(478..672)
                     /locus_tag="VF309_04165"
     CDS             complement(478..672)
                     /locus_tag="VF309_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465814.1"
                     /translation="MLDDIRNHDPRATEKERGWMDTSPVEAAVRVVILASVSIAIGVA
                     ASQSLEAQPHPTTAVAHASR"
     gene            complement(765..1019)
                     /locus_tag="VF309_04170"
     CDS             complement(765..1019)
                     /locus_tag="VF309_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX7465815.1"
                     /translation="MPDDSPNHAVAREARPTMKLTAVYEQVPGGFIAFVEEWCATTAQ
                     GATIEEARQNLRDAFDLTINANRLMSQWRVKGKTVFREPF"
     gene            complement(1009..1215)
                     /locus_tag="VF309_04175"
     CDS             complement(1009..1215)
                     /locus_tag="VF309_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465816.1"
                     /translation="MNSPREDTLAGIRRERLAAAAVLLAIAVSLFGAFIVVVESSSQP
                     ARKHAGAGTGPSRATLDNGEQHAR"
     gene            complement(1280..>1507)
                     /locus_tag="VF309_04180"
     CDS             complement(1280..>1507)
                     /locus_tag="VF309_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465817.1"
                     /translation="MTDGGAAPQDALPQWCWLENPSAQWAPRRSAKAPPAIAADGSGT
                     IDGAVRTERPSMPPGALRNIAIHDDTFSAED"
BASE COUNT          206 a          502 c          544 g          255 t
ORIGIN      
        1 cgcggtcttc accgccgcgg gcttcacggg ctcgggagcc gccttcgcca cggccgcggc
       61 gagatcgcgc cgcgccggcg cggtgaaggc cggctgcgca atcacggcgg gaacgattcg
      121 ctcgagctcg cggatgtgcg acgtgtaggc ctccacgagg catgaatcgg tctggcaggg
      181 attgcgcacg cgctcgagcc acgcatccat ctcggcccgg gtctcatcgc gattggaaga
      241 ggcgcggatc gcgcgctcca cgagggagcc gtggcgcccg tccagctcgg agagctcctg
      301 cgagcggcag atccgcagct cctgcggcga catcacgttc gcgcagtcga agctcggggt
      361 gggatagtcc tgcgcgaagg cgcactgcgt gaaggcgagc gctcctcccg ccgccaggac
      421 cgcccgcatt tccgaccgga ttcgcatccg gccattctcc tactttccgg gcgggcgtca
      481 gcggctcgcg tgggcgacag cggtcgtcgg gtggggttgc gcctccagcg actggctcgc
      541 ggcgacgccg atggcgatcg agacgctcgc gagaatcacg acgcgcaccg ccgcctcgac
      601 ggggctcgtg tccatccaac cgcgctcctt ctcggtggcc ctggggtcgt gattgcgtat
      661 gtcgtcgagc attttcggat ctcctgggtg accataagcc tcgcccgagg cgcgcagcaa
      721 acgaattgtt ttgtcgcgat gccgcgatgc ctttgcggcg tgcctcagaa cggctcgcgg
      781 aagacggtct tgcctttcac gcgccactgc gacatgagcc gattggcgtt gatggtgagg
      841 tcgaacgcgt cgcgcaggtt ctggcgcgcc tcctcgatcg tggcgccctg cgcggtcgtg
      901 gcgcaccatt cctcgacgaa ggcgatgaag ccgccgggca cctgctcgta gaccgccgtg
      961 agcttcatcg tgggccgcgc ctcgcgtgca acggcatggt tgggggagtc atcgggcatg
     1021 ttgttctccg ttatcgaggg ttgcgcggga tgggcccgtg ccggcgcccg catgcttgcg
     1081 cgccggctgt gacgatgatt cgacgaccac gatgaaggcg ccgaacaaac tcacggcgat
     1141 cgcgaggagc accgccgcgg cggcgaggcg ctccctgcga atgcccgcca gggtgtcttc
     1201 ccggggcgaa ttcatgatca gctcgacctg cctctggtag gcgaaggccg gcgcgcgttg
     1261 ggtggccgac ctcggcacgt cagtcttccg ccgagaaggt gtcgtcatgg atggcgatgt
     1321 ttcgcagcgc gccgggcggc atcgagggcc gctcggtcct gacggcgccg tcgatcgtcc
     1381 cggagccgtc cgccgcgatc gcggggggcg ccttggcgga acgccgcggg gcccactggg
     1441 cggacgggtt ctcgagccag caccattggg gaagcgcgtc ctgcggcgcg gcgccgccgt
     1501 cggtcat
//
LOCUS       DASVII010000319         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_518133,
            whole genome shotgun sequence.
ACCESSION   DASVII010000319 DASVII010000000
VERSION     DASVII010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518133"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VF309_04185"
     CDS             <1..245
                     /locus_tag="VF309_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465818.1"
                     /translation="LDEPAAGIPSAESVEVLGVIAALPADVTVLFIEHDMGLVFRFAE
                     RITVLVAGRVLMEGTPEEVARNDEVKAVYLGREAHG"
     gene            238..939
                     /locus_tag="VF309_04190"
     CDS             238..939
                     /locus_tag="VF309_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465819.1"
                     /translation="MAELLRLETVTSGYGESVVVEDASLAVGEGEAIALLGRNGVGKT
                     TLLMTVMGLTRLHRGAIRWRGGDVTRLESHARARAGIGWVPQERFMWKSLTVLEHLTC
                     VARPGPWTVEKVNALFPRLAERAGHRGAQLSGGEQQMLAIARALVTNPGLLLLDEPME
                     GLAPIVVQELTQVLRGLASTSTMAMIVVEQHAELALALTQRAVVMERGRIVHDGPSAA
                     LLADRARLDQWLAVA"
     gene            complement(1048..1953)
                     /locus_tag="VF309_04195"
     CDS             complement(1048..1953)
                     /locus_tag="VF309_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7465820.1"
                     /translation="MKKILKVAAIAGVALIAVGALAFAGAVELGNRKLQRYVDIRVVP
                     VPYSKDAAALKLGKYLFESRGCGECHGDDGRGIVFVDAPNGMYVKAPDITGGKGGVVS
                     DYNEGDWVRTIRHGVNPKGHPLLAMPSEDYNRMNDADFAALVAYVRSLPPVAGEIAVI
                     RLPRFVKALYGVGTIKDAAEKIDHRRPPSQAVAVAASVEHGAYVATMCVGCHGPTFAG
                     GRIPGTPPEWPPAANLTPGEGSVMPRYDTAEKFVAMMRTGRRPDATEVNKVMPFMSLR
                     NLNDTDLNAVYAYLKTLAPRKVAER"
     gene            1992..>2449
                     /locus_tag="VF309_04200"
     CDS             1992..>2449
                     /locus_tag="VF309_04200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7465821.1"
                     /translation="MMEVTSPIRTMRSHSDVPPSPWVLRWASQIRPGGCVLDVAAGGG
                     RHARLLARMGFEVDAVDRDRALFADPPPGVRLLEADIEGGAWPYEGRLFDGIVVANYL
                     HRPLLPLLVGSLEPGGMLIYETFAHGNERFGKPSNPDFLLAPGELLEAVRG"
BASE COUNT          348 a          834 c          849 g          418 t
ORIGIN      
        1 tcctcgacga gccggcggcg ggaattccgt cggccgaaag cgtcgaggtg ctcggcgtga
       61 tcgcggcact tcccgcggac gtgaccgtgc tcttcatcga acatgacatg ggcctcgtgt
      121 tccgcttcgc cgagcgcatc acggttctcg tcgccggccg cgtgctgatg gaaggcacgc
      181 ccgaggaggt tgcgcgaaac gacgaggtga aggcggtcta tctcggcagg gaggcccatg
      241 gctgagctgc tgcgcctcga aaccgtcact tcgggctacg gcgagtcggt cgtggtcgag
      301 gacgcttcgc tcgcggtcgg ggagggcgag gcgatcgcgc tcctcggccg caacggcgtc
      361 ggcaagacga cgcttctcat gacggtgatg ggcctcacgc gcctgcatcg cggcgcgatt
      421 cgctggcgcg gcggcgacgt cacgcgcctc gagtcccacg cgagggcgcg cgccggaatc
      481 ggctgggtcc cccaggagcg cttcatgtgg aagtcgctca ccgtgctcga gcatctcacc
      541 tgcgtcgcgc ggccgggacc gtggaccgtg gagaaagtga acgcgctctt tccacgcctg
      601 gccgaacgtg ccggccatcg cggcgcccag ctctccggcg gcgagcagca gatgctcgcg
      661 atcgcgcggg cgctggtgac caatccgggc ctgctcctcc tcgatgagcc catggaaggc
      721 ctcgcgccga tcgtcgtgca ggagctcacc caggtgctgc gcggcctcgc gagcacctcg
      781 accatggcga tgatcgtcgt cgagcagcac gccgagcttg cgctcgcgct cacccagcgc
      841 gcggtggtga tggagcgcgg gcgcatcgtg cacgacggcc cgagcgcggc cctcctcgcg
      901 gaccgcgcgc gcctggatca gtggctcgcg gtggcttaga aattggtgtc aggtacgcat
      961 ttccgcgcgc accggtttgc cggtgctctc cgccgaaatg cgtacctgac accaatttct
     1021 cctccggctt cattcgatgc cggagcctca gcgctcggca accttgcgcg gcgcgagcgt
     1081 cttcaggtag gcgtacaccg cgttcaggtc cgtgtcgttc aggttgcgca ggctcatgaa
     1141 gggcatcacc ttgttcacct cggttgcgtc gggccgccgg ccggtgcgca tcatcgccac
     1201 gaatttctcc gcggtgtcgt agcgcggcat gacgctgcct tcgcccggcg tgaggttggc
     1261 cgcggggggc cactccggcg gcgtgcccgg gatcctgcct ccggcaaacg tgggcccgtg
     1321 gcaacccacg cacatcgtcg cgacataggc gccgtgctcg acgctggccg cgacggcgac
     1381 ggcctgcgaa ggaggcctgc gatggtcgat cttctccgcg gcatccttga tcgtgcccac
     1441 gccgtagagc gccttcacga acctcggcag gcggatcacc gcgatctcgc ccgcgaccgg
     1501 cggaagcgag cgcacgtacg cgacgagcgc cgcgaagtcg gcgtcgttca tgcggttgta
     1561 gtcctcgctg ggcatcgcga gaagcggatg ccccttcggg ttcacgccat ggcgaatcgt
     1621 gcgcacccag tcgccttcgt tgtagtcgga gacgactccg cccttgccac ccgtgatatc
     1681 gggagccttc acgtacatgc cgttgggcgc gtcgacgaac acgatgccgc gtccatcgtc
     1741 gccgtggcat tcgccgcagc cgcgcgattc gaagagatac ttgccgagct tgagcgcggc
     1801 ggcatccttc gaatagggca cgggcaccac gcggatgtcg acgtagcgct gcagcttgcg
     1861 gttgccgagc tcgacggcgc cggcgaaggc gagggcgccg acggcgatga gcgcgacccc
     1921 ggcgatcgcc gcgaccttca ggatcttctt catcttgcgg tttccatcaa gcgccatggt
     1981 aaccgagcga tatgatggag gtgacgagcc cgatccgcac gatgcgaagc cattccgatg
     2041 ttccgccgtc gccctgggtg ctgcgctggg cgagccagat ccgccccggc ggttgcgtgc
     2101 tcgacgtcgc ggccggcgga ggacgccatg cccggcttct cgcgcgcatg ggattcgagg
     2161 tcgacgcggt cgatcgcgac cgcgcgctct tcgcggatcc gccgccgggg gtgcgcctgc
     2221 tcgaagccga catcgagggc ggcgcgtggc cctacgaggg acggttgttc gacggcatcg
     2281 tcgtcgcgaa ttacctgcac cgtccgctcc tgccgctgct ggtgggctcg ctcgagcccg
     2341 gcgggatgct gatctacgag accttcgcgc acggcaacga gcgcttcggc aagccctcga
     2401 atccggattt cctgctggcc ccgggcgagc tcctcgaggc ggtgcgcgg
//
LOCUS       DASVII010000320         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_698047,
            whole genome shotgun sequence.
ACCESSION   DASVII010000320 DASVII010000000
VERSION     DASVII010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698047"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF309_04205"
     CDS             <1..684
                     /locus_tag="VF309_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465822.1"
                     /translation="TSTVTLSMPAFVQDKKKLTMVAGGLAGLVLVLGILVAYKKPSNV
                     DVAAATQPSTASQPATQPAQAAQSAQSATPPQAEPPKPSEAPKQETKPDAKVLEQSPP
                     ASAIPAPAPAPAPGAETSEKAARDVKKEGEKAAKDKAAAEASSKGETKGETQKPALPP
                     ATLVFAITPWGEIFVNGKSRGVTPPMKSIKLDPGKYKIEIRNTTFAPHAENFDLKARD
                     EVTVRHKFQ"
     gene            690..1538
                     /locus_tag="VF309_04210"
     CDS             690..1538
                     /locus_tag="VF309_04210"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HEX7465823.1"
                     /translation="MKMTLAHRIILAAAGIVVAACQQLPGTDQKPAPASPQITEEVLR
                     ERATEQLATGVKQYEVGEYDNAVKSLTGSLEHGMLSKMQQARARKYLAFSHCVSGREA
                     LCRDEFRKAFEIYSDFSLTAAEDGHPIWGPIYRNVRTQLIAEREASVAKPLIPLGKAE
                     QMLSDGMVKYDGGDYAAAHTLLESALKEGLKKPDDQVRAMKHVAFTQCLLGKYSQCRA
                     DFLKIYDVDPNFDLTPAEAGHPSWTKTFAAAKAQAKKAQEEKEAKEARERSKGPPPTA
                     SVPKKP"
BASE COUNT          359 a          584 c          541 g          210 t
ORIGIN      
        1 acctcgacgg tcacgctcag catgccggcc ttcgtgcagg ataagaagaa actcacgatg
       61 gtcgcgggcg ggctggcggg actggtgctg gtcctcggca tcctcgtcgc gtacaagaaa
      121 ccgagcaacg tcgacgtcgc cgcggccacg cagccctcca cggcctcgca gccggcgacg
      181 caaccggcgc aggccgcgca gtccgcgcaa tcggccacgc cgccgcaggc cgagccgccg
      241 aagccctccg aagcgccgaa gcaggaaacc aagcccgatg cgaaggtgct cgagcagtcg
      301 cctccggctt cggccattcc cgcaccggca cccgccccgg cgccgggcgc ggagacttcg
      361 gaaaaggcgg cgcgcgacgt gaagaaggaa ggcgagaaag ccgccaagga caaggctgca
      421 gccgaagcat cgagcaaggg agaaaccaag ggcgagacac aaaagcccgc attgccgccc
      481 gcgaccctgg tcttcgcgat cacgccctgg ggtgaaatct tcgtcaacgg gaaatcgcgc
      541 ggcgtcacgc cgccgatgaa atcgatcaag ctggatcccg gcaagtacaa gatcgagatc
      601 cgcaacacca cctttgcgcc ccacgcggag aatttcgacc tcaaggcgcg cgacgaggtc
      661 acggtgcggc ataaattcca atgaccacca tgaaaatgac ccttgcgcac cgcatcatcc
      721 tcgccgccgc gggcatcgtc gtcgcggcct gccagcagct gccgggaacc gaccagaagc
      781 ccgcgcccgc ctcgccccag atcaccgagg aggtgctgcg cgagcgcgcc accgagcagc
      841 tcgccacggg cgtgaagcaa tacgaggtcg gcgaatacga caacgcggtg aagagcctca
      901 cgggttcgct cgagcacggc atgctctcca agatgcagca ggcgcgcgcg cgcaagtacc
      961 tcgcgttctc gcactgcgtg tcgggccgcg aggccctgtg ccgcgacgag ttccgcaagg
     1021 ccttcgagat ctattcggac ttctccctca ccgcggcgga ggacgggcac ccgatctggg
     1081 ggccgatcta ccgcaacgtt cgcacgcagc tcatcgccga gcgcgaggca tcggtcgcga
     1141 agccgctcat cccgctcggc aaggccgagc agatgctctc cgacggcatg gtgaagtacg
     1201 acggcggcga ctacgccgcg gcgcacacgc tcctcgaaag cgcgctcaag gaagggctga
     1261 agaagcccga cgaccaggtg cgcgcgatga agcacgtcgc cttcacgcaa tgcctgctcg
     1321 ggaagtactc ccagtgccgc gccgacttcc tcaagatcta cgacgtcgac cccaacttcg
     1381 atctcacgcc ggccgaggcg gggcatccgt cgtggacgaa gaccttcgcg gccgccaagg
     1441 cgcaggcgaa gaaggcgcag gaggagaagg aagcgaagga agcccgggaa cgctcgaagg
     1501 ggccgccgcc cacggcatcg gtgccgaaga agccctagcc gctcaatgat cgcgacgctc
     1561 ggacgctaca agatcgtttc tgaaatcggc cagggggcga tgggtggcgt ctacaaggcc
     1621 gtggatccga tcatcgaccg cacggtcgcg atcaagacca tcaacctcaa cctctcgcgc
     1681 caggagctcg agga
//
LOCUS       DASVII010000321         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_158600,
            whole genome shotgun sequence.
ACCESSION   DASVII010000321 DASVII010000000
VERSION     DASVII010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158600"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VF309_04215"
     CDS             <1..924
                     /locus_tag="VF309_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7465824.1"
                     /translation="LGISDETLVKSVEPQLKLQKERGSDLTVFSPRASGMAHHVGTEE
                     VGQQWARVSNDLIHRICTLLPDNFAPVGQLPQFPGAPIERSIPELERLVNELGFVGVN
                     LNPDPSGGYWKSPPLTDRYWYPIYEKLVELEVPAMIHVTSSCNPAVHATGAHYLNGDT
                     TAFMQLLQGNLFKDFPGLKFVIPHGGGAVPFHWGRYRGLAQDMKKPPLGEHLLENVFF
                     DTCVYHYPGIELMTKVVPVDNMLFASEMLGAVKGIDPETGFHFDDTKRYVDQVKALSD
                     EDRYRIFEGNARRVYPRLDRRLAQRGKPGRK"
     gene            936..1640
                     /locus_tag="VF309_04220"
     CDS             936..1640
                     /locus_tag="VF309_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465825.1"
                     /translation="MNDKTTPPLGDIRRDFERVSADIVKKASGYAASILADVAGRRGT
                     LDARIQPLARSMRMAGPAFTIEVRPGDNLMIHAAITMAKPGDILVVDGKADRTCALMG
                     SIMINACAKLGLGGVILDAAHRDTEELLELGFPVYSLGSNPNGPTKFVPGRVNWPISC
                     GGVSIQPGDLIVGDADGVVVVEREKAGSLLDAAAKKVADERARIADITAGRNLSPKWL
                     EGSLRAAGVLKEGETL"
     gene            1637..2119
                     /locus_tag="VF309_04225"
     CDS             1637..2119
                     /locus_tag="VF309_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465826.1"
                     /translation="MSALAQGARPAFGLPGRIQDANSKPRSIAVVGLGTGGAQVARTL
                     KGEGLDHLEVHVLAKHAPGGDAVAAIKAGGGELQRGLMAADMIFLVACRGDDVGLAPV
                     VAGIARSRNHPVTGLYIVPPEALEGDDETLRTLRSSVEMLVVVSDQSYVPAMIAALGS
                     "
BASE COUNT          397 a          779 c          777 g          353 t
ORIGIN      
        1 ctcggtatca gcgacgagac gctcgtgaag tcggtcgagc cgcagctgaa attgcagaag
       61 gagcgcggca gcgacctcac ggtcttctcg ccgcgcgcct cggggatggc gcaccacgtc
      121 ggcaccgaag aggtcggcca gcaatgggcg cgggtgagca acgatctcat ccatcgcatc
      181 tgcacgctgc tgccggacaa cttcgcgccc gtcggccagc tgccgcagtt tcccggcgcg
      241 ccgatcgagc gctcgatccc cgagctcgag cgcctcgtga acgagctcgg cttcgtgggc
      301 gtgaacctca acccggatcc ctccggcggc tactggaagt cgccgccgct cacggaccgc
      361 tactggtatc cgatctacga gaagctcgtg gagctcgagg tgccggcgat gatccacgtg
      421 acgtcgtcgt gcaatccggc ggtgcacgcc acgggcgcgc attacctcaa cggcgacacc
      481 accgcgttca tgcagctcct gcaaggcaac ctcttcaagg atttccccgg cctcaagttc
      541 gtgatccccc acggcggcgg ggccgtcccg ttccactggg gacgctaccg ggggctcgcg
      601 caggacatga agaagccgcc gctcggcgag cacctgctgg agaatgtctt cttcgacacc
      661 tgcgtctatc attaccccgg catcgagctc atgacgaagg tggtgcccgt cgacaacatg
      721 ctgttcgcct ccgagatgct cggcgccgtg aagggaatcg atcccgagac gggattccat
      781 ttcgacgaca ccaagcgata cgtggaccag gtgaaggcat tgagcgacga ggatcgctac
      841 aggatcttcg agggcaacgc gcgccgcgtg tatccgcgcc tcgaccgccg gctcgcccag
      901 cgcggcaagc cgggccgaaa gtgaaggagg gttcgatgaa cgacaagacc acgccgcccc
      961 tgggagacat tcgccgcgat ttcgagcgcg tatccgccga catcgtgaag aaggcctcgg
     1021 gctatgcggc gtcgatcctc gccgacgtcg ccggccggcg cggcacgctt gacgcgcgta
     1081 tccagccgct ggcgcgctcg atgcgcatgg ccggccccgc gttcacgatc gaggtgcggc
     1141 ccggggacaa tctcatgatc cacgccgcca tcacgatggc gaagcccggc gatatcctgg
     1201 tggtcgatgg aaaggccgac cgcacctgcg ccctgatggg ctcgatcatg atcaacgcgt
     1261 gcgcgaaact gggcctcggc ggcgtgattc tcgacgcggc gcatcgcgac acggaggagc
     1321 ttctggagct gggatttccc gtctactcgc tcggctccaa tccgaacggg cccaccaagt
     1381 tcgtcccggg gcgcgtcaac tggccgatct cgtgcggggg cgtctcgatc cagcccggcg
     1441 acctcatcgt gggcgacgcc gatggagtcg tggtcgtcga gcgcgagaag gccggatccc
     1501 tcctcgacgc cgcagcgaag aaggtggccg acgagcgcgc gcggatcgcc gacatcaccg
     1561 cgggcaggaa cctgagtccc aagtggctcg agggatcgct gcgcgcggcg ggagttctaa
     1621 aggaaggcga gacgctgtga gcgcgctcgc gcagggcgcg cgtcccgcgt tcggattgcc
     1681 cggccgcatc caggacgcca actcgaagcc gcgctccatc gcggtcgtgg ggctcggcac
     1741 cggcggcgcg caggtcgcgc gcacgttgaa gggcgaaggc ctcgatcacc tcgaagtgca
     1801 cgtgctcgcc aagcacgccc ccggaggcga cgcggtggcg gccatcaagg cgggcggcgg
     1861 cgagctccag cgcggcctca tggcggcgga catgatcttc ctcgtcgcgt gccgcggcga
     1921 cgacgtgggc ctcgcgcccg tagtggcggg catcgcgcgc agccgcaacc atccggtcac
     1981 gggcctgtat atagttccgc cggaggcgct cgaaggcgac gacgagacgc tgaggacgtt
     2041 gcgctcgagc gtcgagatgc tcgtggtggt ttcggaccaa agctatgtgc cggcgatgat
     2101 cgccgcgctc ggcagctgaa tcgaggagag tctgcgtgaa cgacatgacc ccgcccacga
     2161 tcgatccacc gtacctctat ccgggatacc gcagcacgct cctgcgctcg ccgaaggaaa
     2221 agctcctcga gcttccgcgg ggcgcgctcg acgttccggg accgctcgtg ccgaagggat
     2281 tcgtgcgcga cggcgacaac gacctc
//
LOCUS       DASVII010000322         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_446445,
            whole genome shotgun sequence.
ACCESSION   DASVII010000322 DASVII010000000
VERSION     DASVII010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446445"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VF309_04230"
     CDS             <1..326
                     /locus_tag="VF309_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7465827.1"
                     /translation="KQRVSIARTVLKNPPILVFDEATSALDSHTEKSIQAELAEISRD
                     RTTLVIAHRLSTIVDADEILVLEHGEIVERGNFRDLLAMNGRFAQMWRLQQEEAESEP
                     IAESR"
     gene            323..1285
                     /locus_tag="VF309_04235"
     CDS             323..1285
                     /locus_tag="VF309_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7465828.1"
                     /translation="MKKLADAVLAIALAVAAGPAPAQEPWPARPVKLVVPSSPGGGTD
                     FFARILAQALGDAMQQQFVVDNRPGASGNVGAQVVASAPRDGYTFLVASNASIAINPA
                     LFGKLAYDAERDLAPVARGVMAINVLVANPATGLKSVNDLVAQAKRQPETLAFGSAGT
                     GSSLYLGVRMIEEAGGVKFIHAPYKGVAPAYQDLLAGRLQFMYTDLASALPYVSSGRL
                     TALAADRKTPLLPGIATFAEAGWPGIDSPTSFSVMAPSGIPPAVLQKMAAEIAKALKM
                     LAPRLEQQGLVPVFDSPAEFAADLAKERASWASFIKRNGITADQ"
     gene            complement(1269..>1702)
                     /locus_tag="VF309_04240"
     CDS             complement(1269..>1702)
                     /locus_tag="VF309_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465829.1"
                     /translation="TVGEAFLAAARDDSPAALDMEAVWGHSRQSQLAQSPVPGTTLLG
                     ASRRLNGRSKAGALSAALEACNRYRVTKEALGALRVPTLVVAGKRDQMTSFKAGKAVA
                     NEIPGARFVALDAGHSMMSEAPHGLLAALKAFLAPDPTDPR"
BASE COUNT          272 a          592 c          572 g          266 t
ORIGIN      
        1 agaagcagcg cgtgtcgatc gcgcgcaccg tcctgaagaa tccgccgatc ctcgtgttcg
       61 acgaggcgac ctcggccctc gactcgcaca cggagaagtc gattcaagcg gagctcgccg
      121 agatctcgcg cgaccgcacg actctcgtca tcgcgcatcg gctgtcgacc atcgtcgacg
      181 ccgacgagat cctcgtgctc gagcacggcg agatcgtgga gcgcggcaat ttccgcgacc
      241 ttctcgccat gaacgggcgc ttcgcgcaga tgtggcgcct gcagcaggaa gaagcggagt
      301 cggagccgat agcggaatcg agatgaagaa gctggccgat gccgtgcttg cgattgccct
      361 tgccgttgcg gcggggcccg cgccggcgca ggaaccctgg cccgcgaggc ccgtcaagct
      421 cgtggtgcct tcgtcgccgg gcggcggcac cgatttcttc gcgcggatcc tcgcccaggc
      481 gctgggcgat gcgatgcagc agcagttcgt cgtcgacaat cgtcccggcg cgagcggcaa
      541 cgtcggcgcc caggtcgtgg cgagcgcgcc acgcgacggc tacaccttcc tggtcgcgtc
      601 gaacgcttcc atcgccatca atcccgcgct cttcggcaag ctcgcctacg acgccgagcg
      661 cgatctcgcc cccgtggccc ggggcgtcat ggcgatcaac gtcctggtcg ccaatcccgc
      721 caccggattg aagagcgtga acgacctggt cgcgcaggcg aagaggcagc cggaaacgct
      781 cgcgttcggt tcggcgggca ctggctcgtc tctttacctg ggtgtccgga tgatcgaaga
      841 ggcgggaggc gtgaagttca tccacgcgcc ctacaagggt gtcgcccccg cgtaccagga
      901 tctgctcgcc gggaggctcc agttcatgta caccgacctc gcgtccgcgc ttccctacgt
      961 gagctccggc aggctcacgg cgctcgcggc cgacaggaaa accccgctcc tcccgggcat
     1021 cgccacgttc gccgaagcgg gatggccggg aatcgattcg ccgacatcgt tcagcgtcat
     1081 ggcgccgagc ggaataccgc ccgcggtgtt gcagaagatg gctgccgaga tcgcgaaggc
     1141 gctgaagatg ctggcgccgc gcctcgagca gcaggggctg gtgccggtat tcgactcgcc
     1201 ggccgagttc gcggcggacc tcgcgaagga gcgcgcgagc tgggcctcgt tcatcaagcg
     1261 caacggcatc accgcggatc agtagggtcc ggggcgagga atgccttcag cgcggcgagc
     1321 agcccgtggg gcgcctcgct catcatcgaa tgccccgcgt cgagcgcgac gaatcgcgcc
     1381 ccggggatct cgttagcgac agcctttccc gccttgaacg aagtcatctg gtcgcgcttg
     1441 cctgcgacca cgagcgtcgg cacgcgcaga gcgccgagcg cttccttcgt cacgcgatag
     1501 cggttgcacg cctcgagtgc tgccgaaagc gcgccggcct tcgatcgccc gttgagccgg
     1561 cggctcgcgc ccagcagggt cgtgcccggc accgggcttt gcgcgagctg ggactggcgc
     1621 gagtgtcccc acaccgcctc catgtcgagc gccgcgggag aatcgtcgcg cgccgccgcg
     1681 agaaacgcct ccccgaccgt ca
//
LOCUS       DASVII010000323         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_410686,
            whole genome shotgun sequence.
ACCESSION   DASVII010000323 DASVII010000000
VERSION     DASVII010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410686"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VF309_04245"
     CDS             <1..951
                     /locus_tag="VF309_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7465830.1"
                     /translation="WRHTAIETRNRETVVIPNGWMLKNRFTVIGSRDDSQASWRRWVR
                     LTVDFSAPPSRVCSVLEAAVRDAEIPHVAEHPAPSAVLLEIGPRYGSYALRYWLDDPQ
                     HDDATDSHVRTHILAALARQGMKLGVQYQEQLDIKDDESHREEQRAGERGRRLAALAS
                     VELFAPLTTVERDELAGHLVYAPFVAGDVITRQGATAHWLYLMVSGEADVWIDAPAGR
                     KAVATLGAGSVFGEMGMMTGEPRHATVGARTDVVCYRLDKSGFESIVRARPDIIGAIS
                     RILAERETELESRRALRQPGAAAAGQSDIHRRIRAFFGLE"
     gene            complement(956..1348)
                     /locus_tag="VF309_04250"
     CDS             complement(956..1348)
                     /locus_tag="VF309_04250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7465831.1"
                     /translation="MFASRMEELKSARAFIEDFCEGAQVAKDSRLKANLVIEELFINT
                     VKHGNRGGSGAPVWITLSASGGEVSLIYEDRAPPFNPFARATREMLEALAETRREGGL
                     GVILAHGLTASADYSYVFGRNRIRLTLA"
     gene            1418..1879
                     /locus_tag="VF309_04255"
     CDS             1418..1879
                     /locus_tag="VF309_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX7465832.1"
                     /translation="MHIPEEKLMRKWIIAAALSVAFPAMAADVGLIKVSKGSVQIQRG
                     AEKMAAAVGSGVQASDVIVTGADGSAGITFTDNSLVSVGPNSVFAIDKYHFDTTTHQG
                     EFEGNLKQGRLAAISGKMVKQSPESMQVRTPSAIMGVRGTEFVVQVGEKTN"
     gene            1887..2489
                     /locus_tag="VF309_04260"
     CDS             1887..2489
                     /locus_tag="VF309_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7465833.1"
                     /translation="MKLSIYLALAALCAGCATAPKHVAPELFAVVPGADGHVGAIVVR
                     TEGDARVIDSAYGAERISSDGTRETTRLTAAEVRDTFGATLAALPGKPTSFVLYFLEG
                     KDELTAESKVEMEKVFSELKRRPLPDIMVIGHTDSVGSMTSNDRLSLARAERMRELMI
                     GLGIPAERIQAAGRGKRELLVPTEDSISEPRNRRVEISVR"
     gene            complement(2486..>3664)
                     /locus_tag="VF309_04265"
     CDS             complement(2486..>3664)
                     /locus_tag="VF309_04265"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7465834.1"
                     /translation="LRHKVVLVGVSGLGVLDYKSTPLGEFIPGVEIHAQVVENLFNGV
                     SLVRPALATWFEGAAFIVCGLVLIWFIPRLSALQGINLVVFLVLLLLGIGLVAFRHLH
                     MLLDFAWPALGTVAVFGTVVVGTLSEADRQRRVLRDQAAHMAGEVDAARRIQMGLLPN
                     PREALGDDRRFKLAALLEPARTVGGDFYDCFHIDSRRIFFVVADVSGKGLPAALFMAS
                     VKSHLKSAALRGGDVGEVLTRAQAEIARENPEQLFVTAFAAILDVPSGVIEFSSAGHE
                     PPFVRTPRGAPERFEIPGGPPLCVIEDFVYAPSRRKLEPGEWICCVTDGATEAMNEQR
                     EFFGVERLRASLSWMPEDIDPEGLVERLRDDVRRFTGDAEPADDITLLVLRWDGGSAA
                     "
BASE COUNT          615 a         1275 c         1258 g          516 t
ORIGIN      
        1 tggcgccaca ccgcgatcga gacgcgcaac cgcgagaccg tcgtcattcc gaatggctgg
       61 atgctcaaga accggttcac ggtgatcggc tcgcgcgacg attcgcaggc gtcctggcgg
      121 cgctgggtcc ggctcaccgt cgatttctcc gcgcctccga gccgcgtgtg ctcggtgctg
      181 gaggccgcgg tgcgggatgc cgagatcccg cacgtcgccg agcatcccgc gccgagcgcc
      241 gtgctgctcg agatcggtcc gcgctacggg agctatgcgc tgcgctactg gctcgacgac
      301 ccgcagcacg acgacgccac cgacagccac gtgcgcacgc acatcctggc ggcgctcgcg
      361 cgccagggca tgaagctggg cgtccagtac caggagcagc tcgacatcaa ggacgacgag
      421 tcgcaccgcg aggagcaacg cgccggggag cgcgggcggc gcctcgccgc gctcgcgtcc
      481 gttgagctct tcgcgccgct caccaccgtc gagcgcgacg agctcgcggg gcacctggtc
      541 tacgccccat tcgtcgcggg cgacgtcatc acgcgccagg gggcaaccgc ccactggctc
      601 tacctcatgg tctccggcga ggccgacgta tggatcgacg cgcccgccgg ccgcaaggcc
      661 gtggccacgc tcggcgcggg aagcgtgttc ggcgagatgg gcatgatgac cggcgagccg
      721 cgccacgcga ccgtgggcgc gcgcaccgac gtcgtctgct atcgcctcga caagtcgggc
      781 ttcgagtcca tcgtcagggc ccgcccggac atcatcgggg ccatttcgcg catcctcgcc
      841 gagcgcgaga ccgagctcga gtcgcggcgc gcgttgcgcc agccgggcgc ggccgcggcg
      901 gggcaatccg acatccaccg gcgcatccgg gcgttcttcg ggctcgaatg acgcttcagg
      961 cgagagtgag ccggatgcgg ttgcgcccga aaacgtacga gtagtcggcc gaggcggtga
     1021 ggccgtgcgc gaggatcacc ccgagcccgc cctcgcggcg cgtctcggcg agcgcctcga
     1081 gcatctcgcg ggtcgcgcgg gcgaacggat tgaagggcgg cgcgcggtcc tcgtagatga
     1141 gcgaaacctc cccgccgctc gccgacagcg tgatccagac gggagccccg cttccgcccc
     1201 ggttgccgtg cttcaccgtg ttgatgaaga gctcctcgat cacgaggttg gccttcagcc
     1261 gcgagtcctt ggcgacctgg gcgccctcgc agaagtcctc gatgaaggcc cgggcgctct
     1321 tcagctcctc catccgggac gcaaacatcc ccattcgcgt agtgacaagg ttcacaggca
     1381 gggctccccg gcttttggca ttatagggtg tcagaacgtg catatcccag aggagaagct
     1441 catgcgcaag tggatcatcg cggcggccct gtccgtcgca ttcccggcta tggcggccga
     1501 cgtaggtctc atcaaggtct cgaagggcag cgtgcagatc cagcggggcg ccgaaaagat
     1561 ggcggcggcc gtggggtcgg gcgtgcaggc ttccgacgtg atcgtcaccg gcgccgacgg
     1621 ctccgcgggt atcaccttca ccgacaacag cctcgtctcc gtcggaccga acagcgtgtt
     1681 cgcgatcgac aagtaccact tcgacaccac tacgcaccag ggcgaattcg agggcaacct
     1741 gaagcaaggg cgccttgccg cgatctcggg caagatggtc aagcagtcgc ccgagtccat
     1801 gcaagtccgc accccttcgg cgatcatggg cgtgcgcggc acggagttcg tggtccaggt
     1861 cggcgagaag acgaactaga caccccttga agctgtcgat ctacctcgcg cttgcggcgc
     1921 tttgcgccgg ctgcgccacg gcgccgaagc atgtggcgcc ggagctgttc gcggtggtcc
     1981 ccggcgccga cggacacgtc ggcgcgatcg tggtgcgcac ggaaggcgac gcgcgcgtca
     2041 tcgattcggc ctacggcgcc gagcgcatct cgagcgacgg cacgcgcgag acgacccgcc
     2101 tcacggccgc cgaggtgcgc gacacgttcg gcgccacgct cgccgccctt cccgggaagc
     2161 ccacgagctt cgtgctctat ttcctcgagg gcaaggacga gctcaccgcg gaatcgaagg
     2221 tcgagatgga aaaggtcttc agcgaattga agcgtaggcc gctcccggac atcatggtga
     2281 tcgggcacac ggactcggtc ggaagcatga cctcgaacga ccgcctgtcg ctcgcgcgcg
     2341 cggagcgcat gcgcgagctg atgatcggcc tgggcattcc cgccgagcgc atccaggccg
     2401 cgggccgcgg caagcgcgag ctcctcgtgc ccaccgaaga cagcatctcc gagccgcgca
     2461 accgccgcgt cgagatcagc gttcgctagg cagcggaccc cccgtcccag cgcagcacca
     2521 gcagcgtgat gtcgtccgcg ggctcggcat cgcccgtgaa gcggcgcacg tcgtcgcgca
     2581 gcctctcgac gaggccctcg ggatcgatgt cctcgggcat ccacgagagc gaggcgcgca
     2641 ggcgctcgac tccgaagaac tcgcgctgct cgttcatcgc ttcagtcgcg ccgtcggtga
     2701 cgcagcagat ccactcgccg ggctcgagct tgcgccggct cggcgcgtag acgaagtcct
     2761 cgatcacgca caggggcggg ccgcccggaa tctcgaagcg ctcgggcgct ccgcgcgggg
     2821 tgcgcacgaa cggcggctcg tggcccgcgc tcgagaactc gatgacgccg ctgggaacgt
     2881 cgaggatcgc cgcgaacgcg gtgacgaaga gctgctcggg attctcgcgc gcgatctcgg
     2941 cctgcgcgcg cgtgagcact tcccccacgt cgccgccgcg aagcgccgcg ctcttcaggt
     3001 gcgatttcac cgacgccatg aacagcgccg cgggcaatcc ctttcccgag acgtcggcga
     3061 ccacgaagaa gatgcggcgc gaatcgatgt ggaagcaatc gtagaagtcg ccgcccacgg
     3121 tgcgcgcggg ctcgagaagc gccgcgagct tgaagcgccg gtcgtcgccg agcgcttcgc
     3181 gcggattcgg caggagcccc atctggatgc gccgcgccgc gtcgacctcg cccgccatgt
     3241 gcgccgcctg gtcgcgcagc acgcggcgct ggcggtcggc ctccgagagc gtgccgacca
     3301 cgacggttcc gaacacggcc acggtgccga gcgcgggcca cgcgaaatcg agcagcatgt
     3361 ggaggtggcg gaacgcgacg agcccgatgc ccagcaggag aagcacgagg aacacgacga
     3421 gattgatccc ctgcagcgcc gaaaggcgcg gaatgaacca gatgaggacc aacccgcaga
     3481 cgatgaaggc ggcgccctcg aaccacgtgg cgagcgccgg gcgcacgagg gagacgccgt
     3541 tgaagagatt ctcgaccacc tgcgcgtgga tctcgacgcc gggaatgaat tcgccgagcg
     3601 gcgtcgactt gtagtcgagc acgcccagcc ccgagacgcc gaccaggacc accttgtggc
     3661 gcag
//
LOCUS       DASVII010000324         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_158657,
            whole genome shotgun sequence.
ACCESSION   DASVII010000324 DASVII010000000
VERSION     DASVII010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158657"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VF309_04270"
     CDS             complement(<1..436)
                     /locus_tag="VF309_04270"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7465835.1"
                     /translation="MKLAHVALDVPLEEAFDFLIPEGQLAPIGSLVVVPFGRTRRVGV
                     VVGLAEGSRVPVERLKSIESVVADIPPIASAELELYDFCARYYQRPLGEVLGTVLPPR
                     LRQVSRRQLVAPGEAGGAAPRLVEAQPLTQEQREAIASCATGS"
     gene            complement(684..1739)
                     /gene="hemE"
                     /locus_tag="VF309_04275"
     CDS             complement(684..1739)
                     /gene="hemE"
                     /locus_tag="VF309_04275"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX7465836.1"
                     /translation="MQNDTFLRAAMREATPYTPIWIMRQAGRYLPEYNETRRKAGSFL
                     ALAKTPELACEVTLQPLRRFALDAAIIFSDILTVPDAMGLGLHFVEGEGPKFANPLRA
                     ERDIMALEAPDLEKLRYVFDAISLTRKELKGRVPLIGFSGSPFTLACYMVEGGGSDDF
                     RLLKTMLYSRPDLMHRILATNARAVIEYLNAQIAAGAQAVMIFDTWGGVLADAAFHEF
                     SLAYSKQVLAGLTRTSEGRVVPRILFTKGGGAWLEAMAQSGADTLGIDWQTHLGDARR
                     RVGSSVGLQGNLDPMALFAPPEAIEREVERVLRSFGAAPGHVFNLGHGVSQYTPPEHV
                     AALVEAVHSRSLGAHAA"
     gene            complement(1793..2479)
                     /locus_tag="VF309_04280"
     CDS             complement(1793..2479)
                     /locus_tag="VF309_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7465837.1"
                     /translation="MPRGWRLENVSFAAADGTPLAGVLALPPIERPPLVIYFGGNAEE
                     VTSFAPRVADLYGERAILLVNYRGYGESGGKPGETALVADGAALFDWAVRRGDFDAAR
                     IALHGRSLGAGVAVQVAAMRPAKCVILTSPFDSALEIAKATYPWLPISLLIRHPFDSM
                     ARAPGIHAPALFLMGTADTLIPMSHSERLAAAWGGAAERVSFEGFGHNDIELNPRYAE
                     TLRSFLDRHL"
     gene            complement(2610..>2886)
                     /locus_tag="VF309_04285"
     CDS             complement(2610..>2886)
                     /locus_tag="VF309_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7465838.1"
                     /translation="TIPAQLVLLAMGYVHPVHEGLVEELGVAKDARGNVKASTDGPGS
                     YATSVAKVFAAGDMRRGQSLVVWAIREGRQCAREVDAFLMGASELPR"
BASE COUNT          512 a          923 c         1018 g          433 t
ORIGIN      
        1 cggatcccgt ggcgcacgac gcgatcgcct cgcgctgctc ctgcgtgagc ggctgggcct
       61 cgacgagtcg cggcgcggcg cctcccgctt cgcccggcgc gacgagctgc cggcgcgaca
      121 cttgccgcaa gcgtggcggc agcacggttc ccaggacttc gccaagcggc ctttggtagt
      181 agcgcgcgca gaagtcgtac agctcgagct cggcgctcgc gatcggagga atgtccgcga
      241 cgaccgattc gatcgacttc agacgttcaa cggggacgcg gctgccctcg gccagcccca
      301 ccacgacacc aacccttcgc gtgcgcccga agggcaccac cacgagcgat ccgatgggtg
      361 ccagctgccc ctcgggaatg aggaaatcga aggcctcttc cagagggaca tccagtgcga
      421 catgggcgag tttcacgcag cgggaaaagc ccggataagc ctgtggataa gtctgtgaat
      481 acgtctgccg cgcaagagcg gcaagcggct cgattcgggc gaaatcttca tggggcttgg
      541 ttcaaatccg ctgcacgcaa ttgaaatcaa acgtgatttc tgggatgtcc tacaggcatg
      601 caaaagtgac gattcgatga cgctagaacg cggggagtgt gaataagcaa tcacatcagc
      661 acaaatattt ttcttgacag agcctatgcc gcgtgggccc cgagggagcg tgaatgcacc
      721 gcctcgacga gcgccgcgac atgctcgggg ggcgtgtatt gggacacgcc atggccgagg
      781 ttgaagacgt ggcccggcgc cgcgccgaat gagcgcagca cgcgctcgac ttcccgctcg
      841 atggcctctg gaggagcgaa gagggccatc ggatcgaggt ttccctgcag gcccacgctc
      901 gagcccacgc gacgccgcgc atcgccgaga tgggtctgcc agtcgatgcc gagagtgtcg
      961 gcgccgctct gcgccatcgc ctcgagccac gccccgccgc ccttggtgaa cagaatgcgc
     1021 ggcacgacgc gcccttcgct cgtgcgcgtg aggcccgcga gcacctgctt cgagtaggcg
     1081 agcgagaact cgtggaacgc ggcatcggcg aggacgccgc cccaggtgtc gaagatcatg
     1141 accgcctgcg cgcccgcggc gatctgtgca ttcaggtatt cgatcacggc gcgggcattg
     1201 gtcgcaagga tgcgatgcat caggtcggga cgcgagtaca gcatcgtctt caggaggcgg
     1261 aagtcgtcgc tgccgccgcc ctcgaccatg tagcaggcga gcgtaaacgg gctcccggag
     1321 aatccgatga gcggaacgcg gcctttcaac tccttgcgcg tgagcgagat cgcgtcgaac
     1381 acgtagcgaa gcttctcgag gtcgggcgct tcgagcgcca tgatgtcgcg ctccgcgcgc
     1441 agcgggttcg cgaatttcgg gccctcgcct tcgacgaaat gaaggccgag ccccatcgcg
     1501 tcgggcaccg tgaggatgtc ggaaaagatg atcgccgcat cgagcgcgaa acggcgcagc
     1561 ggctgcagcg tgacctcgca ggcgagctcg ggcgtcttcg cgagcgcgag gaagcttccg
     1621 gccttgcgcc gcgtctcgtt gtattcggga aggtagcggc ccgcctggcg catgatccag
     1681 atcggcgtgt acggcgtggc ctcgcgcatg gccgcgcgaa ggaaggtgtc gttctgcatc
     1741 gggggatttt ctcacggaca ggcccggtac ccggccgagt cgcgagttcg ggtcagagat
     1801 ggcgatcgag gaacgatcgc agcgtctcgg cgtagcgggg attgagctcg atgtcgttgt
     1861 ggccgaatcc ctcgaaggac acgcgctcgg cggccccgcc ccacgccgcg gcgaggcgtt
     1921 ccgagtggct catcggaatg agcgtatcgg cggtgcccat gaggaatagc gcgggcgcgt
     1981 ggatgcccgg cgcgcgtgcc atcgagtcga aaggatggcg gatgaggagc gagatgggga
     2041 gccacggata ggtcgccttc gcgatctcga gcgcgctgtc gaaaggcgag gtgaggatca
     2101 cgcacttggc gggccgcatc gcggcgacct ggaccgcgac gccggccccg aggctgcggc
     2161 cgtgaagagc gatgcgcgcg gcgtcgaaat cgccgcgccg caccgcccag tcgaatagcg
     2221 ccgcgccatc ggcgaccagc gccgtctcgc ccggcttgcc gccgctctcg ccgtagccgc
     2281 ggtagttgac gaggagaatg gcgcgctcgc cgtagaggtc cgcgacgcgg ggcgcgaagg
     2341 aggtgacctc ttcggcattg ccgccgaagt agatgaccag cgggggacgc tcgatcggcg
     2401 gcagcgcgag cacgccggcg agcggcgtgc cgtcggcggc ggcgaacgag acattctcga
     2461 ggcgccaccc gcgaggcacg gccgggcgtt gcgcggcggg ctgcggatag aagatgaggc
     2521 gttcctggcc gagccaaacg agccccgcga tcgcgagata cccgatgacg ccggcgagga
     2581 ggaggaactt cagcgcggta aaggcgacct caacgcggca gctccgaggc gcccatgagg
     2641 aacgcgtcga cttcgcgggc gcattggcgt ccctcgcgaa tcgcccagac caccagcgat
     2701 tgcccgcggc gcatgtcgcc cgcggcgaaa accttcgcaa cggaagtcgc gtagctgccg
     2761 ggcccgtcgg tcgacgcctt cacgttgccg cgcgcgtcct tcgcgacgcc gagctcctcg
     2821 acgagccctt cgtgcaccgg gtgcacgtaa cccatcgcga ggagaacgag ctgcgccggg
     2881 atggtg
//
LOCUS       DASVII010000325         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_338921,
            whole genome shotgun sequence.
ACCESSION   DASVII010000325 DASVII010000000
VERSION     DASVII010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338921"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VF309_04290"
     CDS             <1..553
                     /locus_tag="VF309_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7465839.1"
                     /translation="DGTIPDRRGSLSIDDEGNPTTRNVLIEDGILRGYLQDRMNSRLM
                     GVPATGSGRRESYAAIPMPRMTNTFMLGGHRKAEEIIASVDRGLYAVNFGGGEVDITS
                     GKFVFSASEAYWIENGKIQYPVKGATLIGNGPDALTRVTMIGDDMCLDPGIGMCGKEG
                     QNIPVGVGQPTIRIEGLTVGGTA"
     gene            607..1086
                     /gene="msrB"
                     /locus_tag="VF309_04295"
     CDS             607..1086
                     /gene="msrB"
                     /locus_tag="VF309_04295"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7465840.1"
                     /translation="MTRRNFVATLGALFFSARAASAAGPQDPGKIEKVRLSDEDWRKR
                     LTPEQYAVLRHESTERPGSSPLNGEHRKGTFACAGCDLALFTSESKFESGTGWPSFFT
                     FIPGRLETKTDFKLVLPRTEYHCARCEGHQGHVFDDGPKPTGKRYCNNGAALKFTPA"
     gene            complement(1134..1481)
                     /locus_tag="VF309_04300"
     CDS             complement(1134..1481)
                     /locus_tag="VF309_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465841.1"
                     /translation="MNIARSALRAPLLGLALCAAAHADDAKVFSVTLLPGDMHEECLA
                     LGAGQKRGYSWKSDTPVDFNIHYHEAGKVSYPVKRDAMRGDGGTFVAKVAQDYCWMWS
                     ARDKAAKLDGKIE"
     gene            1659..>2346
                     /locus_tag="VF309_04305"
     CDS             1659..>2346
                     /locus_tag="VF309_04305"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7465842.1"
                     /translation="MTHYRTDDVRIEQGDELLAPMQVMRELASTDRSEQATFEGRKAV
                     HDILRGADDRLLVVVGPCSIHDYAAALDYAKRLRGERERHEHDLAIVMRVYFEKPRTT
                     VGWKGFINDPRLDETFDMNEGIRLARKLLLEINESGIPCGTEFLDLISPQYIADLIAW
                     GAIGARTTESQSHRQLASGLSCPVGFKNGTDGNIRIAVDAIKAAAARHHFISVTKSGH
                     VAVFKTAGNED"
BASE COUNT          441 a          812 c          725 g          368 t
ORIGIN      
        1 cgacgggacg attcccgatc gtcgcggctc gctgtcgatc gacgacgagg gcaatcccac
       61 gacgcgcaac gttctcatcg aggacggcat cctccgcggc tatctccagg accgcatgaa
      121 ctcgaggctg atgggcgtgc ccgccacggg aagcggccgg cgcgagtcgt acgccgcgat
      181 cccgatgccg cgcatgacca acaccttcat gctgggcggc catcgcaagg ccgaggagat
      241 catcgcttcc gtcgatcgcg gcctctacgc cgtgaacttc gggggcggcg aggtggacat
      301 caccagcggc aagttcgtct tctccgcttc cgaggcgtac tggatcgaga acggcaagat
      361 ccagtacccg gtgaagggcg cgaccctcat cggcaacggg cccgacgcgt tgacgcgcgt
      421 cacgatgatc ggcgacgaca tgtgcctcga cccgggcatc ggcatgtgcg gcaaggaagg
      481 ccagaacatc cccgtgggcg tcggccagcc gaccatccgc atcgagggcc tcaccgtggg
      541 cggcaccgcg taaggccacg agcccgcatc cgactcatgg gcattcgcgt acatcccctc
      601 atgaacatga cccgcagaaa tttcgtcgcg accctaggcg cgctcttctt ctccgcgcga
      661 gcagcctccg ccgccggccc ccaggatcca ggcaagatcg aaaaagtcag gctctccgac
      721 gaggactgga ggaagcgcct cacgcccgag cagtacgcgg tgctgcggca cgagtcgacc
      781 gagcggccgg gctcgagccc gctgaacggc gagcaccgca agggcacgtt cgcctgcgcc
      841 ggatgcgacc tcgcgctctt cacctccgaa tcgaagttcg agagcggcac gggctggccg
      901 agcttcttca cgttcatccc cgggcgcctc gagaccaaga ccgacttcaa gctggtcctg
      961 ccgcgcaccg aataccactg cgcgcgttgc gagggacatc aagggcacgt tttcgacgac
     1021 ggcccgaagc ccacgggcaa gcgctactgc aacaacggcg ccgccctgaa attcactccc
     1081 gcgtaaaaac tggtgtcaga gaccaaatga aaattggtct ctgacaccag tttctactcg
     1141 atcttcccgt cgagcttcgc ggccttgtcg cgcgcgctcc acatccagca gtagtcctgc
     1201 gcgactttcg cgacgaaggt cccgccgtcg ccgcgcatcg cgtcgcgctt cacggggtac
     1261 gagaccttgc ccgcctcgtg atagtggatg ttgaagtcga ccggcgtgtc ggacttccag
     1321 gagtagccgc gcttctggcc cgccccgagc gcgaggcatt cctcgtgcat gtcccccggc
     1381 agcagcgtga cggaaaacac cttcgcgtca tcggcgtggg cggcggcgca aagcgcgagc
     1441 cccaggaggg gcgcgcgcag cgccgagcgg gcgatattca tggactttat gctagcattc
     1501 gaggcatgaa tcaccgggtc ggctactttt tctggttctc ctatctcacg ccgcaggcgg
     1561 tcgagaggat ttgagccgga tacagccaac gaatccacga aaaaccgcca gcccgaaact
     1621 ggcggttttt tgttttccgc gaatgagacg atgagcgcat gacgcactac cgcaccgacg
     1681 acgtccgcat cgagcaggga gacgagcttc tcgcgcccat gcaggtgatg cgcgagctcg
     1741 cctccacgga ccgctcggag caggccacct tcgagggcag gaaggccgtg cacgacatcc
     1801 tgcgcggcgc cgacgaccgc ctcctcgtgg tggtgggccc gtgctcgatc cacgactacg
     1861 cggccgccct cgactacgcg aagcgcctca ggggcgagcg cgagcgccac gagcacgacc
     1921 tcgccatcgt gatgcgcgtc tatttcgaga agccgcgcac caccgtcggc tggaagggct
     1981 tcatcaacga tccgcgactc gacgagacct tcgacatgaa cgaggggatc cggctggcga
     2041 gaaagctcct cctcgagatc aacgaaagcg gcatcccctg cggcacggag ttcctcgacc
     2101 tcatctcgcc gcagtacatc gcggacctca tcgcctgggg cgcgatcggc gcgcgcacca
     2161 ccgagtcgca atcccatcgc cagctcgcct cggggctgtc gtgccccgtc ggcttcaaga
     2221 acggcaccga cggcaacatc cgcatcgcgg tcgacgcgat caaggccgcg gcggcgcgcc
     2281 accatttcat ctcggtcacc aagtcgggcc acgtcgcggt gttcaagacc gcgggcaacg
     2341 aggact
//
LOCUS       DASVII010000326         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_231006,
            whole genome shotgun sequence.
ACCESSION   DASVII010000326 DASVII010000000
VERSION     DASVII010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231006"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF309_04310"
     CDS             complement(<1..382)
                     /locus_tag="VF309_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX7465843.1"
                     /translation="MLAGKSSGSAGSIQPAAHTTKDLSMKKHFWIAAAFAALTLVSGC
                     KSNGHAQNSTQMRSLNAVVDAEPLDVLVDSDVKTSALAFGATSPNVQFDSGTRDVQVR
                     SSTAQTILSDKQISFSSGVNNTLLI"
     gene            580..>1862
                     /locus_tag="VF309_04315"
     CDS             580..>1862
                     /locus_tag="VF309_04315"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7465844.1"
                     /translation="MYEDLCLYIDGEFVKGGGRREQDVLDPATGDVLGKLPHATPADL
                     DRALAAAQRAFEKWRGVSPMEKSALLRRVGQMTRDRANEIGRNITLDMGKPLAEAVGE
                     VTRCADHFDWHAEECRRIYGRVIPPRSPNVRQMVLREPIGVCAAFTPWNFPYNQAIRK
                     ICAAVGAGCTIILKGPEDTPSAVVAIARMFHDAGLPPGVLNIVWGVPSEVSEHLIRSP
                     IVRKISFTGSVPVGKQLAALAGAHMKRVTMELGGHSPVIVCEDADVERAADALAGLKF
                     ANAGQVCVSPNRFYVQTKFFDRFMARFIDKAGKIKVGSGLEADTKMGPLAHERRVPAM
                     MEFVEDATQRGGRVELGGARIGAVGNFFQPTVISNPPDDSKVMTLEPFGPIAPCVPFT
                     DLEEAIRRANSLPYGLSAYAFTTSSKNALRIQNGL"
BASE COUNT          308 a          610 c          629 g          315 t
ORIGIN      
        1 agatgagcag ggtgttgttg acgccgctcg agaacgagat ctgcttgtcg ctgagaatcg
       61 tctgcgcggt cgaggagcgc acctgcacgt cgcgcgtgcc ggaatcgaat tgcacgttgg
      121 gggacgtcgc tccgaaggca agcgcgctcg tcttcacgtc gctgtcgacc agcacgtcga
      181 gcggctccgc gtcgaccacg gcgttcagcg agcgcatctg ggtcgaattc tgggcgtgcc
      241 cgttgctctt gcatcccgat accagcgtca gcgcggcgaa ggctgccgcg atccaaaaat
      301 gcttcttcat cgataggtcc ttggttgtat gggctgccgg ctgaatgctg ccggccgagc
      361 cggaagactt ccccgcaagc atgggacagc attgtgcagg gaataggtag atttgctcaa
      421 acttgtgaac caattgtttc cgggcaatag cttaccgcat cggcgggatc gggcgctttc
      481 gcgcgcccgt tttcgccgtc ccgcgcggcc agggcggatg cgcgccatgc ccgcgttaga
      541 atcggggcta ccccgatcac ggcgtcgcca ggagagtcca tgtacgaaga cctttgcctc
      601 tatatcgacg gcgagttcgt gaaaggcggt ggccgccgcg agcaggacgt cctcgatccg
      661 gcgaccggcg acgtgctcgg aaagcttccg cacgcgacgc ccgcggatct cgaccgcgcc
      721 ctcgccgcgg cccagcgggc cttcgagaag tggcgcggcg tctcgccgat ggaaaagagc
      781 gccctgttgc gccgcgtggg ccagatgacc cgcgatcgcg ccaacgagat cggccgcaac
      841 atcacgctcg acatgggcaa gcccctggcc gaagccgtcg gcgaggtcac gcgctgcgcc
      901 gaccatttcg attggcatgc cgaggaatgc cggcgcatct acggccgcgt gattcccccg
      961 cgcagcccga acgtgcgcca gatggtgctt cgcgagccca tcggcgtgtg cgcggcgttc
     1021 acgccgtgga atttcccgta caaccaggcg atccgcaaga tctgcgcggc ggtgggcgcc
     1081 ggctgcacca taatcctcaa gggacccgag gacacgccga gcgcggtggt cgcgatcgcg
     1141 cgcatgttcc acgacgcggg gcttccgccc ggcgtgctca acatcgtgtg gggcgtgccc
     1201 tccgaggtgt cggagcatct gatccgctcg cccatcgtgc gcaagatctc gttcaccggc
     1261 tcggtgccgg tgggcaagca gctcgccgcg ctcgcgggcg cgcacatgaa gcgcgtcacc
     1321 atggagctgg gcggccactc gccggtgatc gtgtgcgagg acgcggatgt ggaacgcgcc
     1381 gccgacgcgc tcgccgggct caagttcgcc aacgcgggcc aggtgtgcgt atcgccgaac
     1441 cgcttctatg tccagacgaa attcttcgac cgcttcatgg cgcgcttcat cgacaaggcg
     1501 ggaaagatca aggtgggttc gggcctggag gccgacacga agatggggcc gctcgctcac
     1561 gagcggcgcg tgccggcgat gatggaattc gtcgaggacg cgacccagcg cggcgggcgc
     1621 gtggagctgg gcggcgcgcg catcggcgcg gtgggcaact tcttccagcc gaccgtgatc
     1681 agcaatccgc ccgacgacag caaggtgatg acgctcgagc ccttcggccc gatcgccccg
     1741 tgcgtgccgt tcaccgatct cgaggaggcg atccgccgcg ccaattcgct tccctacggt
     1801 ctctcggcct acgcgttcac gacctcgtcg aagaatgcgc tgcggatcca gaacggcctg
     1861 ga
//
LOCUS       DASVII010000327         4345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_698267,
            whole genome shotgun sequence.
ACCESSION   DASVII010000327 DASVII010000000
VERSION     DASVII010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698267"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF309_04320"
     CDS             <1..629
                     /locus_tag="VF309_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7465845.1"
                     /translation="YRVGGHTPLAANVRVIAATHQELESRVKQGLFREDLYHRLNVIR
                     LRLPPLRERREDIAMLARHFLSKSAHDLGVEGKRLTDGALQFFTAQDWPGNVRQLENV
                     CHWVTVMAPGQVVDVKDLPAEVRGESAAAAAGDSDWRRLLEREVASRLARGESDIADP
                     LTRDFETALIVKALQHTGGRRIEAAGLLGIGRNTLTRKVQELKIDTKD"
     gene            complement(636..3296)
                     /locus_tag="VF309_04325"
     CDS             complement(636..3296)
                     /locus_tag="VF309_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7465846.1"
                     /translation="MTTHPQASRQEEEERIARLRAYGVLDPDQELPALDELVRRAQDV
                     AAFPMAWLSFFDGKRERLRARAGISTAYLSREQSFAFGTEPAQAPIFIEDLAATDLQT
                     HCLVAAGPRARFLGILPLIAPDGWVIGTLTVLDRRPRKLREAERTALANIASIAMARL
                     EARRESGAAAASRAATASNRPLAERLEDETRRRRAAEDGLEREREFSEAVLDSLAGAF
                     FLVSSDGVILRWNAALTAAIGYTGAEIGTMQPLDFISPRDRDHAAAAMREVFEQGREM
                     NLEAEIVDRAGNVRPYALSGKPLRVGERTYMIGVARDITLRKRTEQQMARAKERLDLA
                     LTGSRLALWDWDLRNGKVYFNESWSSLLGAPPRESTFSSDEVTQWNHPEDRDVFAAAL
                     GNAMKGVSEEFDCEYRVSNAAGEWIWVHSRGKVTQRDEAGQALRMTGTSHDISKRKRA
                     EERAEYLATRDPLTGLPNRVLLHDRLEQGVFNAARHQAGFAFMFIDLDRFKTINDSLG
                     HQVGDELLKRVAGRLTACVRATDTVARLGGDEFAVILENLEDDDDEGAQQVAEKMIAA
                     MGAPMLIDGQHLSTSCSIGISLYPNDGKDSAALMKNADVAMYYAKEKGRNNYQFFSAE
                     MNSRAQERLSVENFLRLALKRNELALHYQPRMKIATGELVAVEALIRWQHPRRGLLLP
                     EKFIDVAEDSGLIVPIGEWVIEHACAQLRAWQRIVKPDLKVSVNLSVGQVVDAERLYR
                     AVEVALARSGIEPACLELELTESHLMQNIPEKAELLHRLGQLGVGISIDDFGTGYSSL
                     SYLKTLPVDSIKIDSSFVRDIHSDANDEAIIKAILAMAHSLRLAVVAEGVETAAQFRA
                     LKELGCDQYQGFHESPALPPTEFEKRYRVK"
     gene            complement(3293..4069)
                     /gene="xth"
                     /locus_tag="VF309_04330"
     CDS             complement(3293..4069)
                     /gene="xth"
                     /locus_tag="VF309_04330"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7465847.1"
                     /translation="MRVATWNVNSLRVRLPHLLGWLAAHSPDAICLQETKCEDATFPA
                     AELAGAGYCSVHNGQRTYNGVAILSRREGSGVCRGIPRFADDQSRVIAADFEGVRIVS
                     VYVPNGQSVGSEKYAYKLRWFEALEAWLATELAIHPRIAVLGDFNVAPEDRDVHDPAA
                     WEGNVLFSLPERAALARLMGLGFVDGFRLFEQPEKSFTWWDYRMGAFRRNMGLRIDHA
                     LLSPELARACTACTIDVAPRKLERPSDHAPLVCELDIVAA"
     gene            complement(4077..>4345)
                     /locus_tag="VF309_04335"
     CDS             complement(4077..>4345)
                     /locus_tag="VF309_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7465848.1"
                     /translation="LERRGIPYTERDAQNNDADREALKKLVGSLYVPTLVVGEATTKG
                     YDEDLWQAAFDNAGYPKTRLPGQASLRVPPKAAPENTSAEPASK"
BASE COUNT          666 a         1467 c         1502 g          710 t
ORIGIN      
        1 tctatcgcgt cggcggccac acaccgctcg cggccaacgt tcgcgtcatt gccgccacgc
       61 accaggagct cgagtcgcgg gtgaagcagg ggctgttccg cgaggacctc taccatcgcc
      121 tcaacgtgat tcgcctgcgg ctgccgcccc tgagggagcg gcgcgaggac atcgccatgc
      181 tcgcgcggca tttcctgtcg aagagcgccc acgacctcgg cgtcgagggc aagcgactca
      241 ccgacggcgc gctgcagttc ttcaccgcgc aggattggcc cggcaacgtg cgccagctgg
      301 agaacgtgtg tcactgggtc acggtgatgg cgccgggaca ggtcgtcgac gtgaaggatc
      361 tccccgctga ggtgcgcggc gaaagcgccg cggccgcggc gggcgacagc gactggcggc
      421 gcctcctcga gcgcgaggtc gcgagccgcc tcgcccgcgg cgaatcggac atcgccgatc
      481 cgctcacgcg cgatttcgag accgcgctca tcgtgaaggc cctgcagcac acgggcgggc
      541 gccgcatcga ggcggcgggc ctgctcggca tcgggcgcaa cacgctcacg cgcaaggtgc
      601 aggagctcaa gatcgacacg aaggactgaa gcgcgctact tgacccggta gcgcttctcg
      661 aactccgtgg gcggcagcgc cgggctttcg tggaatccct ggtactggtc gcacccgagc
      721 tccttcagcg cgcgaaactg cgccgcggtc tcgacgcctt cggcgacgac cgcaaggcgc
      781 agcgagtgcg ccatcgcgag gatcgccttg atgatggcct cgtcgttcgc gtcggagtgg
      841 atgtcgcgca cgaaggagct gtcgatcttg atcgaatcga cggggagcgt cttcaggtag
      901 gagagcgacg agtagccggt cccgaagtcg tcgatggaaa ttcccacgcc gagctggccg
      961 aggcggtgga gaagctcggc cttctcggga atgttctgca tcaggtgcga ctcggtgagc
     1021 tcgagctcga ggcatgccgg ttcgattccg gaccgcgcca gcgccacctc gactgcgcga
     1081 tacaggcgct ccgcgtcgac cacctgcccg accgaaaggt tcacggagac cttgagatcc
     1141 ggtttcacga tgcgctgcca cgcgcgcagc tgcgcgcacg cgtgctcgat cacccattcg
     1201 cctatcggca cgatgagccc ggaatcctcg gccacgtcga tgaacttttc cggcagcagg
     1261 agtccccggc gcgggtgctg ccagcggatg agcgcctcga cggccaccag ctcgccggtc
     1321 gcgatcttca tgcgcggctg gtagtgcagc gcgagctcgt tcctcttcaa cgcgaggcgc
     1381 aggaagttct cgaccgacag gcgctcctgc gcccgcgagt tcatctcggc ggagaagaac
     1441 tgatagttgt tgcgcccctt ttccttggcg tagtacatcg ccacgtcggc gttcttcatg
     1501 agcgccgcgc tgtccttgcc gtcgttggga tacaggctga tgccgatcga gcacgaggtc
     1561 gagaggtgct ggccgtcgat cagcatcggc gcgcccatcg cggcgatcat cttctcggcg
     1621 acctgctgcg cgccctcgtc gtcgtcgtcc tccaggttct cgaggatcac ggcgaactcg
     1681 tcgccgccaa ggcgcgccac ggtgtcggtc gcgcgcacgc acgcggtgag gcgcccggcc
     1741 acgcgcttta ggagctcgtc tccgacctgg tgccccagcg agtcgttgat cgtcttgaag
     1801 cggtcgaggt cgatgaacat gaaggcgaag ccggcctggt ggcgcgccgc gttgaagacg
     1861 ccctgctcga gccggtcgtg gagcagcacg cggttcggca gccccgtgag cggatcgcgc
     1921 gtggcgaggt attcggcgcg ctcttccgcg cgcttgcgct tggagatgtc gtgggaagtg
     1981 cccgtcatcc gcagcgcctg gcccgcctcg tcgcgctgcg tcaccttgcc gcgggagtgc
     2041 acccagatcc attcacccgc ggcgttagag acccggtact cgcaatcgaa ctcctcgctc
     2101 acgcccttca tggcgttgcc gagggcggcg gcgaagacgt cgcgatcctc gggatggttc
     2161 cactgcgtca cctcgtcgct cgagaaggtc gactcgcgtg gcggcgcgcc gagaagcgac
     2221 gaccagctct cgttgaaata gaccttgccg ttcctgaggt cccagtccca cagcgcgagg
     2281 cgcgagcccg tcagcgcgag atcgagccgc tccttggccc gcgccatctg ctgctcggtg
     2341 cgcttgcgca gcgtgatgtc gcgcgcgacg ccgatcatgt acgtgcgctc tcccacgcgc
     2401 aggggctttc cgctcaacgc gtaggggcgc acattgcccg cgcggtcgac gatctcggcc
     2461 tcgaggttca tctcgcggcc ctgctcgaac acctcgcgca tggcggcggc ggcgtgatca
     2521 cggtcgcgcg gcgaaatgaa atcgaggggc tgcatggtgc cgatctccgc gccggtgtag
     2581 ccgatcgccg ccgtcagggc ggcgttccag cgaaggatca cgccatccga ggagaccagg
     2641 aagaacgctc ccgcgaggct gtcgaggacc gcctccgaga attccctttc gcgctcgagg
     2701 ccgtcctccg ccgcgcgcct gcggcgcgtc tcgtcctcta ggcgctcggc gagcgggcga
     2761 ttggacgcgg tggcggcgcg gcttgccgcg gccgcgccgg attcgcgccg cgcctcgagg
     2821 cgcgccatgg cgatcgaagc gatgttggcg agcgcggtgc gctcggcctc gcgaagcttt
     2881 cgcggccgcc ggtcgaggac ggtgagcgtg ccgatcaccc agccgtcggg cgcgatgagc
     2941 ggcaggatcc cgagaaagcg cgcccgcggg ccggcggcga ccaggcagtg cgtctgcagg
     3001 tccgtcgccg cgagatcctc gatgaagatc ggcgcctgcg ccggctcggt gccgaaggcg
     3061 aacgattgct cgcgcgacag atacgccgtg gagatcccgg cgcgcgcgcg cagccgctcg
     3121 cgcttgccgt cgaagaacga gagccacgcc atcggaaacg cggcgacatc ctgcgcgcgc
     3181 cgcaccagct cgtcgagcgc gggaagctcc tggtcgggat cgaggacgcc gtacgcgcga
     3241 agtcgcgcaa tgcgctcttc ctcttcctgc cggctcgcct gggggtgagt agtcatgcgg
     3301 cgacgatgtc gagctcgcag acgaggggcg cgtggtccga tggccgctcg agcttgcgcg
     3361 gcgcaacgtc gatcgtgcac gccgtgcaag cgcgggcgag ctccggcgaa agaagcgcgt
     3421 ggtcgattcg aagccccatg ttgcgtcgga aggcccccat ccggtagtcc caccatgtga
     3481 acgacttctc gggctgctcg aagaggcgaa agccgtcgac gaatccgaga cccatgaggc
     3541 gcgcgagcgc ggcacgctcg gggaggctga agagcacgtt tccttcccat gcggcgggat
     3601 catgcacgtc gcgatcttcg ggcgcgacat tgaagtcccc gagcacggcg atgcgcggat
     3661 ggatggccag ctcggtcgcg agccacgcct ccagggcctc gaaccagcgc agcttgtacg
     3721 cgtacttttc ggagccgacc gactggccgt tgggcacgta aacgctcacg atgcgcacgc
     3781 cctcgaagtc cgccgcgatc acgcggctct ggtcgtcggc gaatcgcgga atgccgcggc
     3841 aaacgccgct gccctcccgg cgcgaaagga tggccacgcc gttgtaggta cgctgcccgt
     3901 tgtggacgct gcagtatccc gcgccggcga gctcggccgc gggaaacgtc gcgtcctcgc
     3961 atttcgtttc ctggaggcag atcgcatcgg gagagtgcgc cgcaagccag ccgagcagat
     4021 gcggcaggcg cacccggagc gagttcacgt tccaggtcgc gactctcatg cgccgcttat
     4081 ttcgacgcgg gctccgccga ggtgttttcc ggggctgcct tcggaggaac gcgcaaggaa
     4141 gcctgtcccg gcaggcgcgt cttcggatag cccgcgttgt cgaacgcggc ctgccacagg
     4201 tcctcgtcat agcccttggt cgtggcctcg ccgacgacga gcgtcggcac gtagagggag
     4261 ccgaccagtt tcttcagggc ctcccggtcg gcgtcgttgt tctgggcgtc gcgctcggtg
     4321 tacggaatgc cacggcgctc gagga
//
LOCUS       DASVII010000328         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_86910, whole
            genome shotgun sequence.
ACCESSION   DASVII010000328 DASVII010000000
VERSION     DASVII010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86910"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..409)
                     /locus_tag="VF309_04340"
     CDS             complement(119..409)
                     /locus_tag="VF309_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX7465849.1"
                     /translation="MADIDITRAHTLGLSQARTAAESMEEHLGKKFGLKGSWDGDTLH
                     FERPGVTGSLAIAHDKLHLRIALGFMLRAMKGSIEKAVLHELDTLFGGPPAA"
     gene            complement(415..1764)
                     /locus_tag="VF309_04345"
     CDS             complement(415..1764)
                     /locus_tag="VF309_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7465850.1"
                     /translation="MSIVTLGLLFGGITLAVMLTGMPISFALGSVAVVFMYFFMPAAS
                     LNTVTQNVYEEMASITLLSIPLFILKGAAIGRSRAGRDLYAALHAWMSRVPGGLGIAN
                     VFACALFAAMAGSSPATCSAIGQAGIPEMRARGYSPGFAAGIIAAGGTLGILLPPSIT
                     MILYSVAAEVSLGRLFLAGIGPGVLLVTLFAGYAVWRFRKEYSAADAVFKSGGVKSAY
                     LDENALSLRQKVEMLPRVLPFLILLIGVMVALYGGLATPSETAGLGAVLALVLIATIY
                     AMWRPRDLAPILAATLRESTMLMLIIGMSLLYSYVMSYLHLSQSGAQWIVSLHLSKWL
                     LLAAILGLVMVLGFFLPPVSIILMTAPIILPPLKAAGFDLIWFGILMTIVMEMGLIHP
                     PVGLNIFVIRNIAPDIPLRDVIWGVMPFLGLMFLAIFVLCLVPSIATWFPDAVMGAK"
     gene            complement(1761..>2289)
                     /locus_tag="VF309_04350"
     CDS             complement(1761..>2289)
                     /locus_tag="VF309_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7465851.1"
                     /translation="APGTGPEIAPGPLAPVARLLAFVNLWIVRAGSVALLASALILTY
                     SVASRYFFKAATDWQDEAAVFCIVGAIFTASASVQSYRGHIGIELLASFLSPRANRVR
                     AILVDMASFAFCVFFSWKSWTLLHEAYVDNQTTTSSWAPPLWIPYGLMALGITLLALQ
                     IALQVAVALQERGKP"
BASE COUNT          440 a          732 c          806 g          311 t
ORIGIN      
        1 atgtcgctca tgaagatttc ctttcttgct tgtggcaatc ggggaacggg cgacacccga
       61 agcgcgcccg tcacaggcaa cattatacct gcggggacaa cgctttagcc tctggatttc
      121 aagccgccgg cgggccgccg aagagcgtat cgagctcgtg aaggaccgcc ttctcgatcg
      181 agcccttcat ggccctgagc atgaagccga gcgcgatccg caggtggagc ttgtcgtgcg
      241 cgatggcgag cgaccccgtc acccccgggc gctcgaagtg cagcgtgtcg ccgtcccagg
      301 agcccttcag gccgaatttc tttccgaggt gctcctccat gctctcggcg gcagtgcgcg
      361 cctgcgagag cccgagcgtg tgcgcgcggg tgatgtcgat gtcggccatg gcggctactt
      421 ggcgcccatc accgcgtccg gaaaccaggt ggcgatcgag ggcacgaggc agaggacgaa
      481 gatggcgagg aacatgaggc ccaggaacgg catcacgccc cagatcacgt cgcgcagcgg
      541 gatgtccggc gcgatgttgc ggatcacgaa gatgttcagg ccgaccggcg ggtggatgag
      601 gcccatctcc atcacgatcg tcatgaggat gccgaaccag atgaggtcga accccgcggc
      661 cttcagcggc ggcaggatga tgggcgccgt catgaggatg atgctcaccg gcggcaggaa
      721 gaagccgagc accatcacca gcccgaggat cgccgcgagg agcagccact tcgagaggtg
      781 cagcgagacg atccactgcg cgccgctctg gctcaggtgc aggtagctca tcacgtacga
      841 gtagaggagc gacatgccga tgatcagcat gagcatcgtc gattcgcgaa gggtggcggc
      901 gaggatgggg gcgagatccc gggggcgcca catcgcatag atggtcgcga tcagcaccag
      961 ggcgaggacg gcgcccaggc ccgcggtctc cgacggcgtg gcgaggcctc cgtacagcgc
     1021 caccatgacc ccgatgagca ggatcaggaa cggaagcacc cgcggcagca tctcgacctt
     1081 ctggcgcagc gaaagcgcgt tctcgtcgag atacgcggat ttcacgccgc ccgacttgaa
     1141 caccgcgtcg gcggcgctgt attccttgcg gaagcgccac accgcgtagc ccgcgaagag
     1201 cgtgacgagg agcacgccgg gcccgattcc cgcgaggaag agccgcccga gcgacacttc
     1261 cgccgcgacc gaatagagga tcatggtgat ggacggcggc aggagaatgc cgagcgttcc
     1321 gcccgcggcg atgattcccg ccgcgaagcc gggcgaatag ccgcgcgcgc gcatctccgg
     1381 gatgccggcc tggccgatcg cggagcaggt ggctgggctc gatcccgcca tcgcggcgaa
     1441 cagggcgcac gcgaacacgt tcgcgatgcc gaggcctccg gggacgcggc tcatccacgc
     1501 atgcagcgcc gcgtagagat cgcggcccgc gcgcgatcgc ccgattgccg cgcccttcag
     1561 gatgaagagc gggatcgaca gcagcgtgat gctcgccatc tcctcgtaca cgttctgggt
     1621 caccgtgttg agggaagccg cgggcatgaa gaagtacatg aacacgacgg cgacgctccc
     1681 cagcgcgaac gagatcggca tgcccgtgag catcacggcg agcgtgatcc cgccgaacag
     1741 caggcccagg gtgacgatgc tcacggcttg ccgcgctcct gcagggccac ggccacctgc
     1801 agcgcgatct gcagcgcgag gagggtgatg ccgagcgcca tcagcccata cgggatccac
     1861 agcggcggcg cccacgagga cgtggtggtc tggttgtcca cgtaggcctc gtgcaggagg
     1921 gtccacgact tccacgagaa gaacacgcag aaggcgaacg aggccatgtc cacgaggatc
     1981 gcgcgcacgc ggttcgcccg cggcgagagg aaagacgcga ggagctcgat gccgatgtgg
     2041 ccgcggtacg actgcacgga ggcgctcgcc gtgaagatgg cgcccacgat gcagaagacc
     2101 gccgcctcgt cctgccaatc cgtcgcggcc ttgaagaaat agcgggacgc gacgctgtag
     2161 gtgaggatca gggcggaagc gaggagcgcg accgacccgg cccgcacgat ccagaggttg
     2221 acgaacgcga gcagccgcgc gaccggcgcg agcgggcccg gcgcgatctc ggggccggtc
     2281 ccgggggcg
//
LOCUS       DASVII010000329         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_590580,
            whole genome shotgun sequence.
ACCESSION   DASVII010000329 DASVII010000000
VERSION     DASVII010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590580"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VF309_04355"
     CDS             complement(<1..451)
                     /locus_tag="VF309_04355"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HEX7465852.1"
                     /translation="MKLACVACALASGACAAAEDARWYVKIDNDVVFGTDRWYTSGVR
                     IARVKDDLELALVQDIYTPDAKHWHFGVDDRAPTAELLAVGAKHMHGPDYFQTIELAL
                     GVRGPSAVGRQATSDIHRLIPAPEVDWSRQLDDAFDGHVAFTRSQSLG"
     gene            complement(448..1137)
                     /gene="pdsR"
                     /locus_tag="VF309_04360"
     CDS             complement(448..1137)
                     /gene="pdsR"
                     /locus_tag="VF309_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEX7465853.1"
                     /translation="MARRIALVEDDPTIRQNYVDALTRAGYEVAAFGDRASAGKTLAA
                     RLPELAIIDIGLGADIDGGFALCRELRAASDRLPIIFLSARDSDFDIVSGLRLGADDY
                     LTKDVSLPHLLARIAALFRRVDATSQPAVAEDVLNRGRLALDAQRMTATWGGAPVGLT
                     VTEFWMVHSLARHPGHVKDRDQLMRDAEMVVDDGTITSHVKRIRKKFLAVDAAFAAIE
                     TVYGMGYRWIE"
     gene            1329..>3019
                     /locus_tag="VF309_04365"
     CDS             1329..>3019
                     /locus_tag="VF309_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX7465854.1"
                     /translation="MDKRKAAIGDFLKLGMSAFASGLAAAITLAVITLALAGGAQAAS
                     LTDAKTGTLMLATGTAGEFTIAPKVETEVAIQVTGMIARTRLSQVFHNPGTEFVEGVY
                     LFPLPEKAAVDHLWMRIGERVIEGQVQEKEEARRTYEKAKTEGRKAALVEQQRPNLFT
                     NAVAHIGPGENVRVTIEYQQTLAYEKGEYRLRFPLAVTPRYVPAGATDDMPDVPDATG
                     GLANPVDIAVVIDAGMPIANVVSSYHETFVQKEAGNRTAVYLSQEQEAADRDFELKWT
                     VAPGAAPQAAIFTEEIEGAAYALIMVVPPEPNAAQKVAFERVPRETILIVDTSGSMQG
                     ASMEQAKAAVHHALSTLTERDRFNVLEFNSVMHPLYASALPATPQNLANAKAWVSRLK
                     AGGGTEMAPALEFALDGRETPGYLRQVVFMTDGGVSNEDALFKLIERRLGNSRLFTVG
                     IGPAPNGHFMAQAALFGRGTFTQIGDVREVEEKMRALIARIEAPVLQDVSIRWADGSA
                     VDTFPRRVPDLYLGEPIVVSASAAQFAQAITVSGTRGTLPWSVALTSSREGDANGV"
BASE COUNT          492 a         1044 c         1034 g          449 t
ORIGIN      
        1 acccgaggga ctgcgagcgc gtgaacgcga catggccgtc gaacgcgtcg tcgagctggc
       61 gcgaccagtc gacctccggc gcgggaatga ggcgatggat gtcgctcgtc gcctggcgcc
      121 cgaccgcgga ggggccgcgc acgccgagtg ccagctcgat cgtctggaag tagtccgggc
      181 cgtgcatgtg cttcgcgccg accgcgagga gctccgcggt gggcgcccgg tcgtcgacgc
      241 cgaaatgcca gtgcttcgcg tcgggggtgt agatgtcctg gacgagggcg agctcgaggt
      301 cgtccttcac gcgcgcgatg cgcacgccgc tcgtatacca acggtcggtg ccgaacacca
      361 cgtcgttgtc gatcttcacg taccagcgcg cgtcctcggc cgcggcgcag gcgccggagg
      421 ccagcgcgca cgccacgcac gcgagcttca ttcgatccat cgatatccca ttccgtagac
      481 ggtctcgatc gccgcgaagg ccgcgtcgac ggcgaggaat ttcttgcgga tgcgcttcac
      541 gtgcgaggtg atggtgccgt cgtcgaccac catctccgcg tcgcgcatca gctggtcgcg
      601 gtccttcacg tgcccggggt ggcgcgcgag ggaatgcacc atccagaact cggtgacggt
      661 caggcccacg ggcgcgccgc cccaggtcgc cgtcatgcgc tgcgcgtcga gggcgagtcg
      721 gccgcggttc agcacgtcct cggcgaccgc gggctgcgag gtcgcgtcga cgcgccggaa
      781 aagcgcggcg atgcgcgcga ggaggtgcgg caggctcaca tccttggtga ggtagtcgtc
      841 ggcgcccagg cgcagccccg agacgatgtc gaagtccgag tcgcgcgcgg aaagaaagat
      901 gatcggcagg cgatcgctcg ccgcgcgcag ctcacggcac agggcgaatc cgccgtcgat
      961 gtccgcgccc aggccgatgt cgatgatggc gagctccggc agccgcgcgg cgagggtctt
     1021 tcccgcgctc gcccggtcgc cgaatgccgc gacctcatag cccgcgcgcg tcagcgcgtc
     1081 gacatagttc tggcggatgg tggggtcgtc ctcgaccagc gctattcggc gtgccatgga
     1141 gcgcgattat cccgaaaaag tggtgtcagg tacgactttg tcggaattcc gacgaaagtc
     1201 gtacctgaca ccactttttc gccacatttg atcggcgccc tgcccttgca tcgcccgcgc
     1261 gcgtcccccg cgcccgcgcg taatgaccct gcccactctc tcaacgggcg gaaaagggaa
     1321 gacacgggat ggacaagcgc aaggcggcga tcggtgattt tctgaagctg gggatgagcg
     1381 ctttcgcgag cgggctcgcg gcggcgatca cgctggcggt gatcacgctc gcgctcgcgg
     1441 gcggggcgca ggcggcgtcg ctcaccgacg cgaagaccgg cacgctcatg ctcgcgaccg
     1501 gcacggcggg cgaattcacg atcgcgccca aggtcgagac cgaggtcgcg atccaggtca
     1561 ccggcatgat cgcgcgcacg cgcctgtccc aggtgttcca caaccccggc accgagttcg
     1621 tggagggcgt ttacctgttc ccgctcccgg agaaggcggc ggtcgatcac ctgtggatgc
     1681 gcatcggcga gcgcgtgatc gaaggccagg tgcaggagaa ggaggaagcg cgccgcacgt
     1741 acgagaaggc gaagaccgag ggccgcaagg ccgcgttggt cgagcagcag cgccccaacc
     1801 tcttcaccaa cgccgtggcc cacatcggcc cgggcgagaa cgtgcgcgtc accatcgaat
     1861 atcagcaaac gctcgcctac gagaagggcg agtatcgcct gcgcttcccg ctcgccgtca
     1921 cgccgcgcta tgtgccggcc ggcgcgaccg acgacatgcc cgacgtcccg gacgcgaccg
     1981 gcggcctcgc caatcccgtc gacatcgccg tggtgatcga cgccggaatg ccgatcgcca
     2041 acgtcgtgag ctcctaccac gagaccttcg tgcagaagga agcgggcaat cgcaccgccg
     2101 tgtacctctc ccaggagcag gaggccgccg accgcgactt cgaattgaaa tggaccgtgg
     2161 cgccgggcgc cgcaccccag gccgcgatct tcaccgagga gatcgagggc gcggcctatg
     2221 cgctcatcat ggtcgtgccg ccggagccca atgccgcgca gaaggtcgcg ttcgaacgtg
     2281 tgccacgcga gaccatcctc atcgtcgaca cctccggctc gatgcagggc gcttcgatgg
     2341 agcaggccaa ggccgcggtc caccacgcgc tttccacgct caccgagcgc gaccgcttca
     2401 acgtcctcga attcaactcg gtgatgcatc cgctctacgc ctcggcgctc ccggcgacgc
     2461 cgcagaacct cgccaacgcg aaggcgtggg tgtcgaggct caaggccggc ggcggcaccg
     2521 agatggcgcc cgcgctggag ttcgcgctcg acgggcgcga gacgcccggg tacctgcggc
     2581 aagtggtctt catgacagac ggcggcgtct ccaacgagga cgcgctcttc aagctgatcg
     2641 agcggcgcct gggcaactcg cgtctcttca ccgtcggcat cggcccggcg cccaacggcc
     2701 acttcatggc gcaggcggcg ctcttcggac gcggcacgtt cacgcagatc ggcgacgtgc
     2761 gcgaggtcga ggagaagatg cgcgcgctca tcgccaggat cgaggcgccg gtgctgcagg
     2821 acgtctcgat ccgctgggcc gacggcagcg ccgtggacac ctttccgcgc cgcgtccccg
     2881 acctctatct cggcgagccc atcgtcgtct ccgcgtcggc cgcgcagttc gcgcaggcga
     2941 tcaccgtgtc cggcacgcgc ggcaccctac cctggagcgt ggcgctcacg tcttcgcgcg
     3001 aaggcgacgc gaacggcgt
//
LOCUS       DASVII010000330         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_231211,
            whole genome shotgun sequence.
ACCESSION   DASVII010000330 DASVII010000000
VERSION     DASVII010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231211"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1140)
                     /gene="mnmA"
                     /locus_tag="VF309_04370"
                     /pseudo
     CDS             complement(73..1140)
                     /gene="mnmA"
                     /locus_tag="VF309_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
     gene            complement(1217..1579)
                     /locus_tag="VF309_04375"
     CDS             complement(1217..1579)
                     /locus_tag="VF309_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465855.1"
                     /translation="MKKLSALIAMLFASASLSVLAQAPAAPAKPAEPAKPAVAPAPKA
                     DAAPKAEMKKDDVKKKPAKKKATKKSADLTKSGDNPPAAPVTPKAPAEPKKAAGKPAA
                     DITKAGDTPAPAKDAPKK"
     gene            complement(1693..>2056)
                     /locus_tag="VF309_04380"
     CDS             complement(1693..>2056)
                     /locus_tag="VF309_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7465856.1"
                     /translation="VASEAALEALCRGVELNDGPTLPATARAIEAPAGLWERDPPIRV
                     RKSIPTTWLEIGLREGRNRQVRRMTAAVGLPTLRLVRFRVGPWGLEGLQPGRWREVEA
                     TLAPNTRTGARNALRERD"
BASE COUNT          281 a          689 c          721 g          365 t
ORIGIN      
        1 cgacccggtg ctcaggtcgt gggcggagag tctcgagagc cgcccgtcgt tctcctcgac
       61 gcgcccgccg gcttagctga tcacggctcc gccaaggcaa acgtcgcctc gatagagcac
      121 cgccgattgg ccgggcgtga cggcccactg gggactggcg aagtcgagct cgaaggcgcg
      181 cgcgtccgcg ccgcgcaatt cgcacgagga atcggcctgg cgatagcgcg tcttcgcggc
      241 gaatccgccg tcggcgccgg gagcgctgcc gctcacccag ctcgcgtcgg cggcggcgag
      301 attcctcttc aggaggagct cgtggccgtg cccttgcacg accgtgagtg tgttgctggc
      361 aaggtccttc cccgcgacgt accagggctc tcccgcgccg tcgcgccggc ccccgatgcg
      421 aaggccctgg cgttgcccga tcgtgtagta catgagccct tcgtgctctc cgatgcgctc
      481 cccctgcgcg gtcactatcg ggccgggctc gcgcggcagg tagcgggaca ggaactcgcg
      541 gaacgggcgc tcgccgatga agcagatgcc ggtggagtcg cgcttggcgt gattggggag
      601 cccggcctcg cgcgcgatgc ggcgcacctc cggcttctcg agatggccca cggggaacag
      661 ggtgcgcgcg agctgcgcct ggttcagccg atgcaggaag tagctctggt ccttgccgcg
      721 gtcgaggccc ttcaggagaa cgaaccgtcc gtcgcgctcc tcgacgcgcg cgtagtggcc
      781 cgtggcgatg cgctccgcgc ccagggccat cgcgtggtcg aggaaagcct tgaacttgat
      841 ctcggcgttg cacagcacgt cgggattggg cgtgcgcccg gccttgtact cggcgaggaa
      901 actcgcgaac acgcggtcgc gatactcggc ggagaagttg accgcctcga tctcgatgcc
      961 gatccgctcg gcgaccgata ccgcgtcgat gaggtcctcg cgcgtcgagc aataccgatc
     1021 gtcgtcgtcc tcccagttct tcatgaagag gccgaccacg tcgtggcccg cgcgcttcgc
     1081 gaggagcgcg gcgaccgcgg agtcgactcc gcccgaaaga cctacgacga ttttcatgga
     1141 gggttgctgc ttggcgcgag cgtcaaaagc aaaaggcggg gccgaagccc cgccttccgt
     1201 cgtgcgagac gtccgattac ttcttcggcg cgtccttggc cggcgcgggg gtgtcgccgg
     1261 ccttggtgat atccgccgcg ggcttcccgg cggccttctt gggctcggca ggcgccttcg
     1321 gcgtcacggg ggcggccggc gggttgtccc cggacttggt caagtcggcc gacttcttgg
     1381 tggccttctt cttggcgggc ttcttcttca cgtcgtcctt cttcatttcg gccttcggcg
     1441 cggcatcggc cttcggcgcg ggcgcgaccg cgggcttggc gggttccgcg ggcttggccg
     1501 gggcggccgg tgcctgcgcg agcacggaca gtgaagcaga agcgaagagc atggcgatca
     1561 gggcggacag cttcttcatg atgttgttag tttcctaggt tatgttgaac aaggtatcaa
     1621 tggtttcgac tgcgctgaaa tcagcctcgt cgccaacatt aacgccgcgc attataccgg
     1681 ggttgacacg cgtcagtctc gttccctgag agcgtttcgg gccccggtgc gggtgtttgg
     1741 cgccagggtc gcctcgactt cgcgccagcg gccgggctga agcccctcga ggccccaggg
     1801 gcccaccctg aagcgcacga ggcgaagggt gggcagcccc accgccgccg tcatgcgtcg
     1861 cacctggcgg ttcctgccct cgcgcagccc tatctcgagc caggtcgtcg ggatggactt
     1921 gcgcacgcgg atgggcggat cgcgctccca gagcccggcc ggcgcctcga ttgcccgggc
     1981 cgtggccgga agggtgggtc cgtcattcag ctccacgccc cggcacagcg cctcgagggc
     2041 cgcttccgag gcgacg
//
LOCUS       DASVII010000331         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_15791, whole
            genome shotgun sequence.
ACCESSION   DASVII010000331 DASVII010000000
VERSION     DASVII010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15791"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VF309_04385"
     CDS             <1..1413
                     /locus_tag="VF309_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX7465857.1"
                     /translation="GPEWIASLDRLHDHASPVAFDALEPQLLADLGAPVARAFATFDI
                     APSAAGSIAQVHRASLPGGAQVAVKIRRPGVEASIEADVLLLEALATWWEEEQPESRR
                     YQPVEVVRQLRKSLAREVDFSAEARAQERFAESFRDSETIVVPRVHAQFTRPSLLVMD
                     WIDGIPATDLAAVDAAGLDHELLAARGADAVLKMVLVDGFFHADPHPGNVFFLADNRV
                     ALIDFGMVGWLSDKRRDELVDLMAALAARDPDAMRDVLIAWADGERVHAERFSEDIGR
                     MLHLYEHAALREIHLGTLLSEIAAVMREHHLTMPADLALLFKALITLEGLGTRLVPRF
                     RLIEHVTPYVERLVRERWEPRRAAERLSGLTRETGRALRAFPRLLEALARRLGNEDLA
                     IRLEMRELGNFGRQLESSVNRLTIGMVTAALIVGSSILMALSSSQQSFAAWFFGAVGV
                     AISFANSIWLIVSIRRSRRK"
     gene            complement(1417..>1918)
                     /locus_tag="VF309_04390"
     CDS             complement(1417..>1918)
                     /locus_tag="VF309_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEX7465858.1"
                     /translation="HSMKSERGGSRTAGREQGLRERRDDSYREDAKMPDPAACPACRA
                     TYLKGRWTWNDAPAGAASHKCPACRRVEDDFPAGYVTLRGEFLPRHRGEIVDLVMARG
                     ASGKRDHPLQRIIAVKDVAEGLLVTTTDAHLASVIARAVHDAYKGELKLTYSKDENFL
                     RASWTR"
BASE COUNT          255 a          677 c          669 g          317 t
ORIGIN      
        1 ggcccggaat ggatcgcgag cctcgatcgc ctccacgacc atgcgagccc ggtcgccttc
       61 gacgcactcg aaccgcaact gctcgcggac ctcggcgcgc cggtcgcgcg tgccttcgcc
      121 actttcgaca tcgcgccgag cgcggcgggc tcgatcgcgc aggtgcatcg cgcctcgctt
      181 cccggcggcg cgcaagtcgc ggtgaagatc cggcgcccgg gcgtcgaggc ctcgatcgag
      241 gccgatgtgc tcctgctcga agcgctcgcc acctggtggg aggaagagca gcccgagtcg
      301 cggcgctacc agccggtgga ggtcgtacgc cagctgcgca agtcgctcgc gcgcgaggtc
      361 gatttcagcg ccgaggcccg cgcccaggag cgtttcgcgg aaagcttccg cgacagcgag
      421 accatcgtgg tgccgcgcgt gcacgcgcaa ttcacgcgtc cgtcgctcct cgtgatggat
      481 tggatcgacg gcatcccggc gacggatctc gcggccgtgg acgccgcggg cctcgatcac
      541 gagctgctcg ccgcccgcgg cgccgacgcc gtgctgaaga tggtgctggt cgacggcttc
      601 ttccatgcgg atccgcatcc gggcaacgtc ttcttcctcg ccgacaatcg cgtcgcgctg
      661 atcgacttcg gcatggtggg atggctctcc gacaagcggc gcgacgagct cgtggacctg
      721 atggccgcgc tcgccgcgcg cgatcccgac gcgatgcgcg acgtgctgat cgcctgggcg
      781 gatggcgagc gcgtgcacgc ggagcggttc tccgaggaca tcgggcgcat gctgcacctc
      841 tacgagcacg cggcgctgcg cgagatccat ctcggcacgc tgctctcgga aatcgcggcg
      901 gtcatgcgcg agcaccacct gacgatgccc gccgacctgg cgctcctctt caaggcgctc
      961 atcacgctcg agggcctggg cacgcgcctg gtgccgcgct tccggttgat cgagcacgtg
     1021 acgccctacg tggagcggct ggtgcgcgag cgctgggagc cgcggcgcgc ggccgagagg
     1081 ctatcgggcc tcacgcgcga gacggggcgc gccctgcgcg catttccgcg gctgctcgag
     1141 gcgctcgcgc ggcgcctggg caacgaggac ctcgcgatcc gcctcgagat gcgcgagctg
     1201 ggaaacttcg gccgtcagct cgagagcagc gtgaaccgcc tcaccatcgg catggtcacc
     1261 gccgcgctca tcgtgggctc ctcgatcctc atggcgctgt cgagctcgca gcaatccttc
     1321 gccgcgtggt ttttcggcgc ggtgggcgtc gcgatctcct tcgccaacag catctggctc
     1381 atcgtctcca tccgccgctc gcgccgcaag tgatcgctag cgcgtccagc tggcgcgcag
     1441 gaagttctcg tccttgctgt aggtgagctt gagctcgccc ttgtaggcgt cgtgcacggc
     1501 acgggcgatg acgctggcga ggtgcgcgtc ggtggtcgtg acgaggagcc cttccgcgac
     1561 atccttcacg gcgatgatgc gctgcagcgg gtggtcgcgc ttgccgctcg ctccgcgcgc
     1621 catgacgaga tcgacgatct cgccgcgatg ccgcggcagg aattcccccc ggagcgtcac
     1681 gtagccggcg ggaaagtcgt cctcgacgcg gcggcacgcg ggacacttgt gggacgcggc
     1741 gccggcgggc gcgtcgttcc aggtccaacg ccccttcaga taggtcgcgc gacaggcggg
     1801 acaggccgcc ggatcgggca tcttcgcgtc ctcgcggtag ctgtcgtcac gccgctcccg
     1861 cagtccctgt tccctgccgg cggtccggga gccgccgcgt tcgcttttca tggaatgg
//
LOCUS       DASVII010000332         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_446825,
            whole genome shotgun sequence.
ACCESSION   DASVII010000332 DASVII010000000
VERSION     DASVII010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446825"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="VF309_04395"
     CDS             complement(<1..1791)
                     /locus_tag="VF309_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465859.1"
                     /translation="MHTVHTSALAEIDRAARAIAGKADIEVRGPRSGFDDALFAAAAG
                     RAEVAVASPVVEFEAAFADRPGALRILGIDPMRAVRLQPAFVADRAMTGAPQASALLE
                     ASTLWLSPAAASRLGAREGDTLRLVSGSSAIEWRVAGTLPAMDAAGELAVMDIAAAQA
                     RFDRVGRISHIDLRLRAGVDAERFRASLAAMLPPGVVASSAASLSGRAADISRAYRVN
                     LDALALVALATGAFLVFSTLALQAARRQQEFALLRALGVTRRGVATLLALEGALIGGL
                     GAAAGTLVGLAGSRELLARAGGDLGAGFFSAQDVAFAPDYAALAFIAALGIAMSIGGA
                     LWVARAAGRIVVAEALRDRAIDLPTTASASPWPALAFFAAGIPLLFLPPIAGLPLGGY
                     GTITLWLAASVAAVGPSCRALLRVIDPREAAIVSLALAQVRHLPGHLAASVAGIVVSA
                     SLCVAMAIMVFSFRVSLEDWLAGVVGADLYVQSSSGASAGYFSLDEQARIAALPEVRS
                     VDALRQERLALDSHGPPLSLIARPINARILAGFQAEPREMPGAGAIPVWISEAARDIY
                     GWKAGDRISLPIAGRATPVQVAGVIRDYART"
BASE COUNT          284 a          665 c          665 g          189 t
ORIGIN      
        1 cgtgcgcgcg tagtcgcgaa tgaccccggc gacttgcacc ggcgtcgcgc gccccgcgat
       61 cggcagcgag attcgatcgc ccgccttcca tccgtagatg tcgcgcgccg cctccgagat
      121 ccacaccggg atcgcgccgg ccccgggcat ctctcgcggc tcggcctgga agcccgcgag
      181 gatgcgcgca ttgatgggac gcgcgatgag gctcaggggc ggcccgtgag aatcgagcgc
      241 gagccgctcc tgccggagag cgtcgaccga tcgaacctcg ggaagcgccg cgatgcgagc
      301 ctgctcgtcg agcgagaagt aaccggcgct cgcgcccgac gacgactgca cgtagaggtc
      361 ggcgcccacc acgcccgcga gccaatcctc gagcgagacg cgaaacgaaa agaccatgat
      421 cgccatcgcg acgcaaagcg aggcgctgac gacgatgccg gcgacgctcg cggcgagatg
      481 gccgggaagg tggcgcacct gggccagcgc gagcgagacg atcgcggcct cgcgcgggtc
      541 gatgacgcgc aggagcgcgc ggcacgaggg accgaccgcc gccaccgagg cggcgagcca
      601 cagcgtgatc gtgccgtagc ctccgagcgg caatcccgcg atcggcggca ggaagagcag
      661 cggaattccc gcggcgaaga aggcgagcgc gggccatggg ctcgcgctcg ccgtggtggg
      721 caggtcgatg gcgcgatcgc gcagcgcctc cgcgacgacg atgcgcccgg cggcccgcgc
      781 gacccatagc gcgccgccga tcgacatggc gattccgagc gccgcaatga acgcgagcgc
      841 cgcgtagtcc ggcgcgaagg cgacgtcctg ggcgctgaag aatcccgcgc cgagatcgcc
      901 tccggcccgc gcgagaagct cgcggctccc cgcgagcccc accagagtgc ccgccgccgc
      961 gcccaggccg ccgataagcg cgccttccag cgcgagcagg gttgccactc cgcgccgcgt
     1021 cacgccgagg gcgcgcagca gcgcgaattc ctgctgtcgc cgcgccgcct gcagcgcgag
     1081 cgtcgagaag accaggaagg ctcccgtggc gagggcgacg agggccagcg catcgaggtt
     1141 cacgcggtac gcccgcgaga tgtcggccgc gcgcccggag agcgacgccg cgctcgacgc
     1201 gaccacgccc ggcggcagca tcgcggcgag gctcgcgcga aagcgctccg catcgacgcc
     1261 cgcgcgcagg cgcaggtcga tgtgcgagat ccggccgacg cgatcgaagc gcgcctgcgc
     1321 ggccgcgatg tccatcaccg cgagctcgcc cgccgcatcc atcgcgggca gcgtgcccgc
     1381 gacgcgccac tcgatcgccg agcttcccga gacgaggcgc agcgtgtcgc cttcgcgcgc
     1441 tcccaatcgc gacgcggccg ccggcgagag ccacagggtg ctcgcctcga gaagggcgga
     1501 ggcctgcggc gcgcccgtca tcgccctgtc cgcgacgaat gcgggctgca ggcgcacggc
     1561 gcgcatggga tcgatgccca ggatgcgcag cgcgccgggc cggtccgcga aggcggcttc
     1621 gaattcgacg acggggctcg ccaccgccac ttcggcgcgg cccgcggccg cggcgaagag
     1681 cgcgtcgtcg aagccgctgc ggggcccgcg gacctcgatg tcggcttttc cggcgatggc
     1741 gcgcgccgcg cgatcgatct cggcgagcgc agaggtatga accgtgtgca ccgctccggc
     1801 gag
//
LOCUS       DASVII010000333         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_194849,
            whole genome shotgun sequence.
ACCESSION   DASVII010000333 DASVII010000000
VERSION     DASVII010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194849"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..>1785)
                     /gene="ppk1"
                     /locus_tag="VF309_04400"
     CDS             complement(16..>1785)
                     /gene="ppk1"
                     /locus_tag="VF309_04400"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX7465860.1"
                     /translation="EILPALAKEKIRFLRRAEWTPAQQEWVRDYFFREMMPVLTPIGL
                     DPAHPFPRLLNKSLNFVVELEGKDAFGRKSSMAIVQAPRVLPRVIHLPKEMSTGPNDF
                     IFLTSILHAHVGELFAGMNVLSCCQFRVTRNSDLFVEEEEVKDLRKALQGELPQRHFG
                     DAVRLEAADNMTEGMTGFLLSQFGLEAEDLYAVAGPVNLVRLMSIPEQVNRPDLEYPP
                     FAPGVHKALAKGRDLFHVIRKSDVLLHRPFQSFAPVIEFIRQAARDPQVVAMKQTVYR
                     TGTDSVIMQTLIEAARAGKEVTVVVELMARFDEEANINWAARLEEVGAHVVYGVVGHK
                     THAKMALVVRREEGKLVRYVHLGTGNYHPRTARLYTDFDLLTCNEQICADVSEVFQQL
                     TGLGKATKLRHVWQAPFTLHQKVLEAIENETRIARTGKPARIAAKMNALLEPDVIEAL
                     YRASQAGVKVDLVIRGVCALRPGVQGISENIRVRSIIGRFLEHTRIFHFRNGGDSQVY
                     LSSADWMDRNFFRRIELSFPVIDPKLKRRVIREGLKPYLDDNTQAWEMDANGQYKRRT
                     PRRAKPHSAQLRLLAELALAAAK"
BASE COUNT          286 a          585 c          599 g          315 t
ORIGIN      
        1 cggcgtaagc gcagctcact tggcggcggc gagcgccagc tctgcgagca ggcgcagctg
       61 cgcggaatgc ggtttcgcgc gccgcggggt cctgcgcttg tactgtccgt tcgcatccat
      121 ctcccacgcc tgcgtgttgt cgtcgagata gggcttcagg ccctcgcgga tcacgcggcg
      181 cttgagcttc ggatcgatga ccgggaacga cagctcgatg cgccggaaga agttgcggtc
      241 catccagtcg gcgctcgaca ggtagacctg cgaatcgccg ccgttgcgga agtggaagat
      301 gcgcgtgtgc tcgaggaagc ggccgatgat cgagcgcacg cggatgtttt ccgagatgcc
      361 ctgcactccc gggcgcagag cgcacacccc gcggatcacg aggtcgacct tgacccccgc
      421 ctgcgaggcg cgatagaggg cctcgatcac gtcgggctcg aggagcgcgt tcatcttcgc
      481 ggcgatgcgc gcgggcttgc cggtcctcgc gatgcgcgtc tcgttctcga tcgcctccag
      541 gaccttctgg tgcaaggtga aaggcgcctg ccaaacgtgg cgcagcttgg tcgccttgcc
      601 gaggcccgtg agctgctgga acacctcgct cacgtcggcg cagatctgct cgttgcacgt
      661 gagcaggtcg aaatcggtat agaggcgcgc ggtgcgcgga tggtagttgc cggtgccgag
      721 gtggacatag cgcacgagct tgccttcctc gcgccggacc acgagggcca tcttcgcgtg
      781 ggtcttgtgg cccaccacgc cgtacaccac gtgcgcgccc acttcctcga ggcgtgccgc
      841 ccaattgatg ttcgcctcct cgtcgaagcg cgccatgagc tccaccacca ccgtcacctc
      901 cttcccggcg cgggcggcct cgatgagggt ctgcatgatg acggagtcgg tgccggtgcg
      961 atacacggtc tgcttcatcg cgacgacctg cggatccctc gcggcctggc ggatgaactc
     1021 gatcaccggc gcgaacgact ggaacggccg gtgcagcagg acgtcgctct tgcggatcac
     1081 gtggaagaga tcgcgcccct tcgccagcgc cttgtgaacc cccggggcga acggcggata
     1141 ttcgaggtcg ggacgattca cctgctcggg gatgctcatg aggcgcacga ggttgaccgg
     1201 ccccgccacc gcgtacaggt cctcggcctc gaggccgaac tgcgacagga ggaagcccgt
     1261 catcccctcg gtcatgttgt cggcggcttc caggcgcacc gcgtcgccga agtggcgctg
     1321 cggcaactcg ccctggagcg ccttgcgcag gtccttcacc tcctcctcct cgacgaagag
     1381 atcggagttg cgcgtcacgc ggaactggca acagctgagc acgttcatgc cggcgaagag
     1441 ctcgcccacg tgcgcgtgca ggatcgaggt gaggaagatg aagtcgttgg gcccggtcga
     1501 catctccttc ggcaggtgga tcacgcgcgg cagcacgcgc ggcgcctgca cgatcgccat
     1561 gctcgatttg cggccgaacg cgtccttgcc ttcgagctcg accacgaaat tgaggctctt
     1621 gttgagcagg cgcggaaacg ggtgcgcggg atcgagcccg atcggcgtga gcaccggcat
     1681 catctcgcgg aaaaaatagt cgcgcaccca ttcctgctgc gccggcgtcc attcggcgcg
     1741 cctcaggaag cggatttttt ccttcgcgag cgcgggaagg atctc
//
LOCUS       DASVII010000334         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_123552,
            whole genome shotgun sequence.
ACCESSION   DASVII010000334 DASVII010000000
VERSION     DASVII010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123552"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VF309_04405"
     CDS             complement(<1..508)
                     /locus_tag="VF309_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465861.1"
                     /translation="MRAGFIHGIRFRLVMAALVLFAIPWLAAQFISRMESFLRASQEQ
                     AIGATARAVAAALSDRPTLFAPVGAEADPEGEERRRIVALFAAADPDAAASLGNAYVP
                     SEEIERFLAIMGRRSSRLWVVDTRSRVRGLSGALREGATKPVASWLKPVAALVVPAPR
                     LPESDETRP"
     gene            complement(516..1364)
                     /locus_tag="VF309_04410"
     CDS             complement(516..1364)
                     /locus_tag="VF309_04410"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HEX7465862.1"
                     /translation="MKVVALLVACAANAHASADEAHFYTQIANDVAFGTDRWYTSGVR
                     MAREKDGIEWGLVQDIYTPDAKNWHPGKDDRAPTARLLGSGALHQRTPELWQTIEFAI
                     GVQGPAALGHQTTSAVHHFVPAPHVDWTRQPGGRLDAQVAITRSQRVVFDFAKVHYGA
                     TLGTVTTFAHAGFELRAGDTTLSSALLRFAPTPPTSTGSAYAWSAYAGMSERVMGRDE
                     LISRNYDPLGPDLKYRRPLSRFAAGLAWKWHWGALAFDLVQDAKEFNAQTAPQRFGSV
                     SILIDF"
     gene            complement(1361..>1925)
                     /locus_tag="VF309_04415"
     CDS             complement(1361..>1925)
                     /locus_tag="VF309_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7465863.1"
                     /translation="AARLPELAIIDIGLGADIDGGFSLCRELRAASERLPIIFLSARD
                     SDFDIVSGLRLGADDYLTKDVSLPHLLARIAALFRRVDATSQPATAAEVLNRGPLALD
                     AQRMMASWNGHSVGLTVTEFWMVHSLARHPGHVKDREQLMRDAEMVVDDGTITSHVKR
                     IRKKFLAIDPKFDSIDTVYGMGYRWRE"
BASE COUNT          302 a          656 c          685 g          282 t
ORIGIN      
        1 ccggacgcgt ctcgtcgctc tcgggaaggc gcggcgccgg gacgaccagc gccgcgacgg
       61 gcttcagcca actcgcgacc ggcttcgtcg ccccctcgcg caacgcgccc gagaggccgc
      121 gcacgcgcga gcgagtgtcg accacccaca ggcgcgacga gcgccttccc atgatggcga
      181 ggaagcgctc gatctcctcg gacggcacgt aggcgttgcc caggctcgcc gcggcgtcgg
      241 gatcggcggc ggcgaagagc gccacgatgc gccggcgctc ttcgccctcg ggatcggcct
      301 cggcgcccac cggcgcaaaa agcgtgggtc gatccgaaag cgccgcggcg acggcgcgcg
      361 ccgtcgcgcc gatcgcctgc tcctgggacg cgcggaggaa cgattccatg cgcgagatga
      421 attgcgccgc gagccagggg atcgcgaaga gcaccaatgc cgccatcacg agccggaagc
      481 ggatgccgtg aatgaagccg gcgcgcatca ccgcgctaga agtcgatgag gatcgagacg
      541 gagccgaagc gttgtggcgc cgtctgcgcg ttgaattcct tcgcgtcctg caccaggtcg
      601 aaggcgagcg cgccccaatg ccatttccag gccagacccg ccgcgaagcg cgacagcggg
      661 cgccggtact tgaggtcggg cccgagggga tcgtagttgc gcgagatgag ctcgtcgcgg
      721 cccatcacgc gctcgctcat cccggcgtag gcgctccagg cgtaggcgct tcccgtggag
      781 gtgggcggcg tgggcgcgaa gcgcagaagc gcgctcgata gcgtggtgtc gccggcgcga
      841 agctcgaagc ccgcgtgcgc gaaggtcgtc accgtgccga gcgtggcgcc gtagtggacc
      901 ttcgcgaaat cgaagaccac gcgttgagac cgggtgatcg cgacctgcgc gtcgagccgg
      961 cccccgggct gccgcgtcca gtcgacgtgg ggcgcgggaa cgaagtggtg gaccgcgctc
     1021 gtcgtctggt gcccgagcgc cgcggggccc tgcaccccga tcgcgaattc gatcgtctgc
     1081 cacagttccg gagtgcgctg gtgcagcgcg ccggagccga gcaggcgcgc ggtgggcgcg
     1141 cgatcgtcct tcccgggatg ccagttcttg gcgtccggcg tgtatatgtc ctgcacgagc
     1201 ccccactcga ttccgtcctt ctcgcgcgcc atgcgaacgc cgctcgtgta ccagcgatcg
     1261 gttccgaacg cgacgtcgtt cgcgatctgc gtgtagaagt gggcttcgtc cgccgacgca
     1321 tgggcattcg ccgcgcacgc gacgagcaat gccacgacct tcattctcgc catcgatagc
     1381 ccatgccgta gacggtgtcg atggaatcga acttcgggtc gatcgcgagg aacttcttgc
     1441 ggatgcgctt cacgtgcgac gtgatggtgc cgtcgtcgac caccatctcg gcgtcgcgca
     1501 tcagctgctc gcggtccttc acgtgcccgg gatggcgcgc cagcgaatgc accatccaga
     1561 actcggtcac ggtgagcccc acggaatggc cgttccagct cgccatcatt cgctgggcat
     1621 cgagcgcgag aggcccgcgg ttgagcacct cggccgccgt ggccggttgc gacgtcgcat
     1681 cgacgcggcg aaagagcgcc gcgatgcggg cgagcaggtg cggcaggctc acgtccttgg
     1741 tgaggtagtc gtcggcgccg agccgaagcc ccgagacgat gtcgaaatcc gaatcgcgcg
     1801 cggacaggaa gatgatcggc aggcgctcgc tcgccgcgcg cagctcccgg cacagcgaga
     1861 agccgccgtc gatgtccgcg cccaggccga tgtcgatgat cgcgagctcc ggcaggcgcg
     1921 cggcg
//
LOCUS       DASVII010000335         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_87182, whole
            genome shotgun sequence.
ACCESSION   DASVII010000335 DASVII010000000
VERSION     DASVII010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87182"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VF309_04420"
     CDS             complement(<1..171)
                     /locus_tag="VF309_04420"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX7465864.1"
                     /translation="MVPHLTTSLTGPLQELERTVLGERPEIERWFRTQWLEHDVPFYA
                     SVDLRNSGFKLAP"
     gene            306..659
                     /locus_tag="VF309_04425"
     CDS             306..659
                     /locus_tag="VF309_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX7465865.1"
                     /translation="MTPGQTIAATVALLVASNVFMTFAWYGHLKHLQTTSWFVAAMAS
                     WGIALFEYLLQVPANRIGFEGGITLAQLKITQEVITLTVFVPFAMLYMGQGFKWDYLW
                     AALCMVGAVYFIFKG"
     gene            684..>1797
                     /gene="mpl"
                     /locus_tag="VF309_04430"
     CDS             684..>1797
                     /gene="mpl"
                     /locus_tag="VF309_04430"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX7465866.1"
                     /translation="MHLHILGICGTFMGGLAKIAAERGHTVTGCDAQVYPPMSEQLRA
                     IGIKLHEGYDAEQLDRYPADLYVVGNAMTRGKPVIEAILDRGLAYASGPQWLSENVLR
                     DKWVLAVAGTHGKSTTASMLAWILAEGGLDPGFLIGGIARNFDASARATDSSFFVIEA
                     DEYDTAFFDKRSKFVWYRPRTAVLGNLEYDHADIFPDLAAIETQFHHLVRIIPSQGLL
                     VVNGQDAALERVLARGAWTPAERFGAGQPWSVGTIHPDDSFDVLLAGERQGTVRWTLM
                     GEHNRLNALAALAAARHVGIPVKGGIDALGRFEGVKRRMELKGTVGGVTVYDDFAHHP
                     TAVAETLAGLRAANPTSRIWAIFEPRSASSCRRIFQE"
BASE COUNT          287 a          607 c          598 g          305 t
ORIGIN      
        1 gggagcgagc ttgaagccgc tgttgcgcag gtcgaccgac gcgtagaagg gcacgtcgtg
       61 ctcgagccat tgggtgcgga accagcgctc gatctccggg cgctcgccga gcacggtgcg
      121 ctcgagctcc tgcagcgggc cggtgagcga cgtggtgagg tggggaacca tgatggactg
      181 ggccattgga tcggactttt ggatcggact ctggacaacg cgcgggaggg tcagtttaca
      241 ctcttcgcaa tgctagaatt ccccctctcg cagcccgtca ttccggggca aggacgacca
      301 tttcgatgac cccagggcaa acgatcgcgg cgaccgtggc gctcctcgtc gcctccaacg
      361 tgttcatgac ttttgcctgg tatggacacc tgaagcacct gcagacgacc tcgtggttcg
      421 tggctgccat ggcgagctgg ggcatcgcgc ttttcgagta cctgctgcag gtcccggcca
      481 accgcatcgg cttcgagggc ggcatcacgc tcgcccagct gaagatcacg caggaggtca
      541 tcaccctcac cgtgttcgtg cccttcgcga tgctctacat gggccagggc ttcaagtggg
      601 actacctttg ggcggcgttg tgcatggtgg gcgccgtgta tttcatcttc aagggctagg
      661 cggcccacgg cgaagcgcaa ggcatgcacc tccatatcct cggcatctgc ggcactttca
      721 tgggcggcct cgccaagatc gcggccgaac gcggccacac ggtcacgggc tgcgatgcgc
      781 aggtctaccc gccgatgagc gagcagctgc gcgcgatcgg catcaagctg cacgagggct
      841 acgacgccga gcagctcgac cgctatccgg cggacctcta cgtggtcggc aacgcgatga
      901 cgcgcggcaa gccggtgatc gaagcgatcc tcgaccgcgg gctcgcctat gcctcgggac
      961 cgcaatggct ctccgagaac gtgcttcgcg acaagtgggt gctcgccgtc gccggcaccc
     1021 acggcaagag cacgaccgct tcgatgctcg cgtggatcct cgccgagggc gggctcgatc
     1081 ccgggttcct gatcggcggc atcgcgcgca acttcgacgc ctcggcccgc gcgaccgact
     1141 cgtccttctt cgtgatcgag gccgacgagt acgacaccgc gttcttcgac aagcgctcga
     1201 agttcgtctg gtaccgcccg cgcaccgccg tcctcggcaa cctcgagtac gaccacgcgg
     1261 acatctttcc cgacctcgcc gccatcgaga cgcagttcca tcacctcgtg aggatcattc
     1321 cctcgcaggg gctcctggtg gtgaacggcc aggacgcggc gctcgagcgc gtgctcgcgc
     1381 gcggcgcgtg gacgcccgcc gagcgcttcg gcgcgggcca gccgtggagc gtgggaacca
     1441 tccaccccga cgactcgttc gacgtgctgc tcgcgggcga gaggcagggc acggtgcgat
     1501 ggaccctcat gggcgagcac aaccggctga acgcgctcgc cgcgctcgcc gccgcccgcc
     1561 acgtgggaat tcccgtcaag ggcgggatcg acgccctcgg acgcttcgag ggcgtgaagc
     1621 ggcgcatgga gctcaagggc accgtgggcg gcgtgaccgt ttacgacgac ttcgcccatc
     1681 atccgacggc cgtggccgag acgctggcgg gtctcagggc cgcgaacccc acgtcgcgca
     1741 tctgggccat cttcgagccg cgctccgcct cgtcgtgccg gcggattttt caggagg
//
LOCUS       DASVII010000336         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_375450,
            whole genome shotgun sequence.
ACCESSION   DASVII010000336 DASVII010000000
VERSION     DASVII010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375450"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VF309_04435"
     CDS             <1..936
                     /locus_tag="VF309_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7465867.1"
                     /translation="YALAMEEIAAGCASTATLMSVHNSVGCGPILAWGSDAQKKAWLP
                     DMASGRKVGCFCLTEPQAGSEANNLRTRAVLKDGRWVLNGNKQFVTNGKRARVAIVFA
                     VTDPDLGKKGLSAFIVPTQTRGFTPQKLENKLGIRASDTCAIVLEDCAVAEENLLGPR
                     GKGLAIALSNLEGGRIGIAAQALGIARAAFEAALAYAKDRTQFGKKLVEHAPIGGYLA
                     DMHTRINAARLLILHAARMRTEGIPCLSEASQAKLYASELAEWVCSKAIQIHGGYGYL
                     HDYPVERHYRDARITQIYEGTSEIQRLLIARNLSE"
     gene            1067..1384
                     /locus_tag="VF309_04440"
     CDS             1067..1384
                     /locus_tag="VF309_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465868.1"
                     /translation="MDPEWMRYGVYAILAAAAPALWYVRRFKRTLDADIAAANSRGGV
                     ADPQLRSELLRKLQIGGALCELPLALGALHLMAGAERRSFIAGACVSLALRLSYRPFT
                     ANR"
     gene            1384..>1976
                     /locus_tag="VF309_04445"
     CDS             1384..>1976
                     /locus_tag="VF309_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7465869.1"
                     /translation="MPRIKSRLDPGAPAFKANVAHHRALAEDLRKHVARISEGGGAAA
                     QQKHAARGKLLPRERVRALLDPGSPFLELSQLAAFGMYDDDAPSAGIITGIGRVEGRE
                     VVIVANDATVKGGTYYPMTVKKHLRAQEIALQNRLPCVYLVDSGGAFLPLQDEVFPDK
                     EHFGRIFYNQANLSALGVPQVAVVMGSCTAGGAYVPA"
BASE COUNT          315 a          718 c          651 g          292 t
ORIGIN      
        1 tacgcgctcg ccatggaaga gatcgccgcg ggctgcgcgt cgacggcgac cctcatgagc
       61 gtgcacaact ccgtcggctg cggcccgatc ctcgcgtggg gaagcgacgc gcagaagaag
      121 gcgtggcttc ccgacatggc ctcggggcgc aaggtcggct gcttctgcct caccgagccg
      181 caggcgggaa gcgaggccaa caatctcagg acgcgcgcgg tgctgaagga tggccgctgg
      241 gtgctgaacg gcaacaagca gttcgtcacc aacggcaagc gcgcccgggt cgcgatcgtc
      301 ttcgcggtga ccgatccgga cctcggcaag aagggacttt ccgccttcat cgttccgacg
      361 caaacgcgcg gcttcacgcc gcagaagctc gagaacaagc tcggcatccg cgcctcggac
      421 acctgcgcca tcgtgctcga ggactgcgcg gttgcggagg agaacctgtt gggaccgcgc
      481 ggcaagggat tggccatcgc gctctccaac ctcgagggcg gcaggatcgg catcgccgcc
      541 caggcgctcg gcatcgcgcg cgccgcgttc gaagccgccc tcgcctacgc gaaggaccga
      601 acccagttcg ggaagaagct cgtggagcac gcgcccatcg gcggctacct cgccgacatg
      661 cacacgcgca tcaacgcggc gcggctcctc atcctgcatg cggcgcgcat gcgcaccgaa
      721 ggcatcccgt gcctctcgga ggcgtcgcag gcgaagctct acgcgtccga gctggccgag
      781 tgggtatgct ccaaggcgat ccagatccac ggcggctacg gctacctgca cgactatccc
      841 gtcgagcgcc actatcgcga cgcgcgcatc acgcagatct acgaaggcac cagcgagatc
      901 cagcggctgc tcatcgcgcg caacctgtcc gagtagaacc ctggcgatcc cacccaagac
      961 ttcgccccag ccggtcctcg tctggggaac ccagctcgcg ggcaccgccg cggtcgcgat
     1021 cgccatctac ctgttcttcg gccatggctc gccggtattc gcgaacgtgg atcccgagtg
     1081 gatgcgctac ggcgtctacg cgatcctcgc ggcagccgcg ccggcgctct ggtatgtgcg
     1141 ccgcttcaag cgcacgctcg acgcggacat cgcggcggcg aattcgcgcg gcggagtcgc
     1201 ggatccgcag ctgcgctccg agctgctgcg caagctccag atcgggggcg cgctctgcga
     1261 gctgcccctc gcgctcggcg ccctgcacct catggcgggc gccgagcggc gcagcttcat
     1321 cgccggcgcg tgcgtgagcc tcgcgttgag gctctcctac cgcccgttca cggcgaaccg
     1381 ctgatgcccc gcatcaagtc gcgactcgat cccggcgctc ccgccttcaa ggcgaacgtc
     1441 gcgcaccacc gcgcgctcgc cgaggatctg cgcaagcacg tcgcgcggat ctccgagggc
     1501 ggcggcgccg cggcgcagca gaagcacgcc gcccgcggca agctccttcc ccgcgaacgc
     1561 gtgcgcgccc tcctcgaccc gggatcgccc ttcctcgagc tgtcgcagct cgcggcgttc
     1621 ggcatgtacg acgacgatgc gccgtcggcg gggatcatca cgggcatcgg ccgcgtcgag
     1681 ggccgcgagg tcgtgatcgt cgcgaacgac gcgacggtga agggcggcac gtactacccg
     1741 atgacggtga agaagcacct gcgggcgcag gagatcgcgc tgcagaatcg cctgccgtgc
     1801 gtgtacctcg tcgattccgg cggcgccttc ctgcccctcc aagacgaggt ctttcccgac
     1861 aaggagcact tcgggcgcat cttctacaac caggccaacc tctccgcgct cggcgtgccc
     1921 caggtcgccg tggtgatggg atcgtgcacc gccggtggcg cctacgttcc cgcgat
//
LOCUS       DASVII010000337         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_662850,
            whole genome shotgun sequence.
ACCESSION   DASVII010000337 DASVII010000000
VERSION     DASVII010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662850"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VF309_04450"
     CDS             complement(<1..1092)
                     /locus_tag="VF309_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7465870.1"
                     /translation="MRQHNKKSQIESSMQDSPGDPSVKFVTSAPAPAAVKGRSGSLSP
                     EEKLSGDMLMLTTGFMMCAAALWLAVYWSMGYQYSTAIPLIFQGLSVITIGLWWKFRR
                     LYTFAVIQLGLILFTPFAMQWSIGNFVNASGVSLWGLMAPVGAVTVLGTRQSMPWFFA
                     WLFMTVMAGVFDYMLSGTVHKFDLQTVAVFFVLNFAAISVMIYSLLWYFASEKQKLRL
                     QVDAQYEEIRLEKDRSERLLLNILPPAIAERLKRNEINIAQGHADVTVMFADIVGFTR
                     MTEELSPVETVKILNDVFSIFDDIADKHRVEKIKTIGDAYMACAGLDTGAQIHYADAV
                     ASMALEMIQKVDEYRKRTGERLQIRIGIGT"
     gene            1186..>2048
                     /locus_tag="VF309_04455"
     CDS             1186..>2048
                     /locus_tag="VF309_04455"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7465871.1"
                     /translation="MSMSDRDGFIWYDGKLVPWREANTHVLTHSLHYGLAVFEGVRAY
                     KTEIGTAIFRLKEHTKRMFNSAKIYMMEIPYTPEQLMDAQREVVRANKLESCYLRPLA
                     FYGSEKMGVSPVGAKVHVTVAAWPWGAYLGEDGLKKGIRVKTSSFARHHVNVTMARAK
                     VATTYANSILANLEATQHGYDEGLLLDTDGFVAEGCGENIFIVKDGRVYTPELTTALD
                     GITWRSVQSICTDLGIVVEKTRLTRDDIYICDEAFFTGTAAEVTPIRELDRRVIGAGS
                     RGPVTERIQSA"
BASE COUNT          379 a          675 c          644 g          350 t
ORIGIN      
        1 ggtgccgatg ccgatacgga tctgcaggcg ctcgccggtg cgcttgcggt actcgtcgac
       61 cttctggatc atctcgagcg ccatgctcgc caccgcgtcg gcgtagtgga tctgggcgcc
      121 ggtgtcgagg cccgcgcacg ccatgtacgc gtcgccgatg gtcttgatct tctcgacgcg
      181 gtgcttgtcg gcgatgtcgt cgaagatcga gaagacgtcg ttcaggatct tcaccgtctc
      241 caccggcgag agctcctcgg tcatgcgcgt gaaccccacg atgtcggcga acatcacggt
      301 cacgtccgca tggccctgcg cgatgttgat ctcgtttcgc ttgaggcgct cggcgatcgc
      361 cggcggcagg atgttgagga ggagccgctc gctcctgtcc ttctcgaggc ggatctcctc
      421 gtactgcgcg tccacctgca ggcgcagctt ctgcttctcg ctcgcgaagt accagagcag
      481 cgaatagatc atcaccgaga tcgccgcgaa gttgagcacg aagaagaccg cgaccgtctg
      541 caggtcgaac ttgtgcaccg tccccgacag catgtagtcg aagacgccgg ccatcaccgt
      601 catgaagagc caggcgaaga accacggcat cgactggcgc gtgccgagca ccgtcaccgc
      661 gcccaccggc gccatgaggc cccagaggct cacgcccgac gcgttcacga agttgccgat
      721 cgaccattgc atcgcgaacg gcgtgaacag gatgaggccc agctggatca ccgcgaaggt
      781 gtagaggcgg cggaacttcc accacagccc gatggtgatg accgacaggc cctggaagat
      841 cagcggaatc gcggtggaat actggtagcc catgctccag tagacggcga gccagagggc
      901 ggcggcgcac atcatgaagc ccgtggtgag catcagcatg tcgccggaaa gcttctcctc
      961 gggcgacaag gaacccgatc gccccttcac ggccgccggg gccggggcgc tggtgacgaa
     1021 cttgaccgac gggtcccctg gagaatcctg catgctcgat tcgatctgcg acttcttatt
     1081 gtgctgccgc aatgagggcg attatggcat agaacccgcg gcggctgtcc ccgtgatttg
     1141 ggctagaatt ccctctctga gcactcattt ccggagcgac attccatgtc gatgtcagac
     1201 cgcgacggct tcatctggta cgacggcaag ctcgtgccat ggcgcgaggc caacacccac
     1261 gtcctcaccc attcgctgca ctacggcctg gccgtcttcg agggcgtgcg cgcctacaag
     1321 accgagatcg gcacggcgat cttccggctg aaggagcaca cgaagcggat gttcaactcc
     1381 gcgaagatct acatgatgga gattccgtac acgcccgagc agctcatgga cgcgcagcgc
     1441 gaggtggtgc gcgcgaacaa gctcgagagc tgctacctgc gcccgctcgc cttctacggc
     1501 tcggagaaga tgggcgtgtc tccggtcgga gccaaggtcc acgtcaccgt cgccgcgtgg
     1561 ccgtggggcg cgtacctcgg cgaggacgga ttgaagaagg gcatccgcgt gaagacgtcg
     1621 tccttcgcgc gccaccacgt gaacgtcacg atggcccgcg ccaaggtcgc gaccacctac
     1681 gcgaactcga tccttgccaa cctcgaagcc acgcagcacg gctacgacga gggtctcctc
     1741 ctcgacaccg acggcttcgt cgccgagggc tgcggcgaga acatcttcat cgtgaaggac
     1801 gggcgcgtct acacccccga gctcaccacg gcgctggacg gcatcacctg gcgctcggtg
     1861 cagtcgatct gcacggacct gggcatcgtg gttgagaaga cgcgcctcac gcgcgacgac
     1921 atctacatct gcgacgaggc attcttcacc ggcaccgccg cggaagtcac tccgatccgc
     1981 gagctcgacc ggcgcgtcat cggcgcgggc tcgcgcggac ccgtgaccga gcgcatccag
     2041 tcggcgtt
//
LOCUS       DASVII010000338         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_339323,
            whole genome shotgun sequence.
ACCESSION   DASVII010000338 DASVII010000000
VERSION     DASVII010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339323"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VF309_04460"
     CDS             complement(<1..1029)
                     /locus_tag="VF309_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465872.1"
                     /translation="MSCHTATDRHTMHQNPGVILGCTDCHGGDARIEARDEGRDSAPY
                     LALLRKAHVLPRDEKAWHWPSSATPERTFTLLNRETPEFIRFMNPGDLRAAREACGAC
                     HLAVIHATERSLMATSAMFWGAAAYNNGILPYKHSILGEAYTRDGNPASIVNPVKPDT
                     FLASKGILPSLTAMPSWETIPPSDVFRVFERGGRIASTQFPEIGLPNSSGALQKLDEP
                     GRPDIRQSNRGPGTGSRIAVPVINITKTRLNDPHLWFLGTSDQPGDYRSSGCSACHSI
                     YANDRDEKHSGIYARFGHNGETATRDPAIPKSEPGHPIRHEFTRAIPSSQCMVCHMHQ
                     PNVFVNSYY"
     gene            complement(1272..>2668)
                     /locus_tag="VF309_04465"
     CDS             complement(1272..>2668)
                     /locus_tag="VF309_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465873.1"
                     /translation="PTPVGPQTSERPGSFDVFGRGRQSTLSETLIVSMALIKGDTTFK
                     PPDYEFRVVPAFNVNRSQVDEVRALRVDPREGTTRTDNHVGLQEAFADVHLRNVSPRY
                     DFDSVRVGIQPFTSDFRGFLFEDVPFGIRLFGNRDNNRWQYNLAAFRRLEKDTNSGLN
                     DVTTRMRRDYVMVANAYRQDFPWLGFTSQATAVLNVNREGAGQFYDANGFLVRPAVLG
                     DSRPHDYRVAYLGYSGDGHWGRWNFTGTAYAALGHDDRNPLAARPQEIRAGFAAAEVS
                     RDFSWVRLRLNALYQSGDKNPFDGKATGFDAILENPQFAGAETSFWMRQAVPLIGGGG
                     VSLSGRNGILASLRSSKDQGQSNFVNPGLLLLGVGADADVTPQLRAIGNVSYLRFQET
                     AVLGVLRNQAPPSREIGWDVSAAVQWRPFMSQNVVFNFSAAALMPGKGLKQLYDEGQR
                     GPQYSVLGNLLLTF"
BASE COUNT          436 a          835 c          925 g          472 t
ORIGIN      
        1 gtagtagctg ttcacgaaca cgttgggctg gtgcatgtgg cacaccatgc attgcgacga
       61 cgggatcgcc cgcgtgaact cgtggcggat cggatgaccg ggctcgctct tcggaatcgc
      121 cggatcgcgc gtcgcggtct cgccgttgtg gccgaagcgc gcgtagatgc cagagtgctt
      181 ctcgtcgcgg tcgttcgcgt agatcgagtg gcaggcgctg cagcccgagg agcggtagtc
      241 gccgggctgg tcgctcgtgc cgaggaacca caggtgcgga tcgttcaggc gcgtcttggt
      301 gatgttgatg acgggcaccg cgatgcggct tcccgtgccg ggcccgcgat tcgattggcg
      361 tatgtcggga cggccgggct cgtcgagctt ctgcagggcc ccggagctgt tgggcagccc
      421 gatctcggga aactgcgtgg acgcgatccg cccgcctcgc tcgaacaccc ggaacacgtc
      481 ggagggcgga atggtttccc acgacggcat cgccgtgagc gacggcagga tgcctttcga
      541 cgcgaggaag gtatcgggct tcacgggatt cacgatcgac gcggggttgc cgtcgcgcgt
      601 gtaggcctcc ccgaggatcg agtgcttgta gggaaggatg ccgttgttgt acgccgccgc
      661 gccccagaac atcgcggaag tcgccatgag gcttctctcc gtggcgtgga tcaccgcgag
      721 gtggcacgcg ccgcaggcct cgcgcgccgc gcgaaggtcg ccgggattca tgaagcggat
      781 gaactccgga gtttcccggt tgagcagcgt gaatgtccgc tcgggagtcg cgctcgatgg
      841 ccaatgccac gccttctcgt cccggggcag cacgtgggcc ttgcgcagga gcgcgaggta
      901 cggggcgctg tcgcgcccct cgtccctcgc ctcgatgcgc gcgtcgccgc cgtggcaatc
      961 cgtgcagccg aggatgacgc ccgggttctg gtgcatcgtg tggcgatcgg tcgccgtgtg
     1021 gcagctcatg cagccgatcg attttgcgtc cgcttgcgcc accgtctgcg ccgccggcgc
     1081 gggcggcgtt tccgctatcg ctgcacaaga catgcacacg cagaacatgg agatcgcacg
     1141 gagcacacgg aggcaaaccg cgccaaatat cgatcgtgcc cctcgatcca tgtccacgca
     1201 tttcgccatc aaggatgttc tccgtgatct ccgtgcgaac tccgtgtgct ccgtgtgatc
     1261 gtcttgaatc atcagaaggt gagcaacagg ttgccgagga cagagtattg ggggccgcgc
     1321 tggccttcgt cgtagagttg cttcaggccc ttgcctggca tgagcgcggc ggcggagaag
     1381 ttgaacacca cgttctggct catgaagggc cgccattgca ccgcggcgga aacgtcccat
     1441 ccgatctcgc gcgacggcgg cgcctggttg cgaagcaccc ccagcacggc ggtctcctgg
     1501 aagcgcaggt acgagacatt gccaatggcg cgaagctgcg gcgtgacgtc ggcatcggcg
     1561 ccgacgccga gcagcagcaa ccccggattc acgaaattgg attgcccctg gtccttcgac
     1621 gagcgcaggc tcgcgagaat tccgttgcgc cccgagagcg acacgccacc cccgccgatg
     1681 agggggacgg cctggcgcat ccagaagctc gtctcggctc cggcaaactg cggattctcg
     1741 aggatcgcgt cgaagccggt cgcctttccg tcgaaaggat tcttgtcgcc cgattggtag
     1801 agggcgttca ggcgcaggcg cacccagctg aaatcgcgcg aaacctcggc cgcggcgaag
     1861 ccggcgcgga tttcctgggg gcgcgccgcc agcggattgc gatcgtcgtg cccgagcgcc
     1921 gcgtacgccg tcccggtgaa gttccatcgt ccccaatgcc catcgccgct gtatcccaga
     1981 taggcgacgc ggtagtcgtg ggggcgcgaa tcgccgagca ccgcgggacg aacgaggaag
     2041 ccgttcgcgt cgtagaactg tccggcgccc tcgcggttca cgttcaggac cgcggtggcc
     2101 tgggaggtga agccgagcca cggaaaatcc tggcgatagg cgttcgcgac catcacatag
     2161 tcgcgtcgca tgcgcgtcgt cacgtcgttc aagcccgagt tcgtgtcctt ctcgaggcgg
     2221 cgaaacgcgg cgaggttgta ttgccagcgg ttgttgtcgc ggttgccgaa cagccttatg
     2281 ccgaacggca cgtcctcgaa gaggaatccg cggaagtcgg aggtgaaagg ctggatgccg
     2341 acgcgcaccg aatcgaaatc gtagcgcggc gagacattgc gcaagtggac gtcggcgaag
     2401 gcttcctgca gtcccacgtg gttgtcggtg cgcgtcgttc cctcgcgcgg atcgactcgc
     2461 agggcgcgca cctcgtcgac ttgcgagcgg ttcacgttga acgcggggac gacgcggaac
     2521 tcgtagtcgg gcggcttgaa cgtcgtgtcg cccttgatga gcgccatcga gacgatgagc
     2581 gtttccgaaa gcgtcgactg ccgcccgcga ccgaagacgt cgaagcttcc ggggcgctcg
     2641 ctcgtctggg gcccgaccgg cgtcggca
//
LOCUS       DASVII010000339         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_303156,
            whole genome shotgun sequence.
ACCESSION   DASVII010000339 DASVII010000000
VERSION     DASVII010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303156"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>1645
                     /gene="dnaE2"
                     /locus_tag="VF309_04470"
     CDS             30..>1645
                     /gene="dnaE2"
                     /locus_tag="VF309_04470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="DNA polymerase involved in damage-induced
                     mutagenesis and translesion synthesis. It is not the major
                     replicative DNA polymerase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX7465874.1"
                     /translation="MFERFVSPERREPPDIDVDFEHERREEVIQWIYDTYGRDRAAIC
                     ATVIRYRGRGAVRDVGKAMGLSEDTVAALAGMVWGTTSGGELPEKHVRAAGLDPADPL
                     LTAVLELTHELMGFPRHLSQHVGGFVLMRGPLVEVVPVGNAAMQDRTFIEWDKDDIAA
                     LGLLKVDVLGLGMLTCIRRAFDLIEKHEGKSLTLASLPREDAAVYDMLCRADSIGVFQ
                     VESRAQMNMLPRLKPRCFYDLVIEVAIVRPGPIQGDMVHPYLRRRSGVEPEHYPSPSP
                     AHGPKNELENILGKTKGVPLFQEQAMRIALVAAKFSDAEVNELRKAMATFRRRGTIAL
                     LEEKMVSRMVSRGYPPDFAQRCFDQIKGFGEYGFPESHAASFAHLVYVSAWIKCHYPA
                     AFAAALLNSQPMGFYAPAQIVRDAREHEVEARSADVNLSDWDCTLENMEGAGPALRLG
                     LRQIDGAQEEEIKKLMAARPFRDVHDLWLRSGVKLVTLERLAAADAFRSLGLDRRQAL
                     WEVKGLSSAVPLPLFSWSETGEAGLEPAVAL"
BASE COUNT          265 a          569 c          544 g          267 t
ORIGIN      
        1 ccgccgtaaa cccgaccgaa gtcgacctgt tgttcgagcg attcgtgtcg ccggagcgcc
       61 gcgagccgcc cgacatcgac gtcgacttcg agcacgagcg ccgcgaagaa gtcatccagt
      121 ggatctacga cacctacggc cgcgaccgcg ccgcgatatg cgccaccgtc atccgctacc
      181 gcggccgcgg cgccgtgcgc gatgtcggca aggccatggg gctatcggag gacaccgttg
      241 ccgcgctcgc cggcatggtc tggggcacga cgagcggcgg cgagctcccc gaaaagcatg
      301 tgcgcgccgc gggcctcgac cccgccgatc cactgctcac ggctgtgctc gaattgacgc
      361 acgagctcat gggcttcccg cgccacctct cccagcacgt cggcggcttc gtcttgatgc
      421 gcgggccgct ggtcgaggtc gtccccgtcg gcaacgcggc catgcaggac cgcaccttca
      481 tcgagtggga caaggacgac atcgccgcgc tcgggctttt gaaggtcgac gttctgggcc
      541 tcggcatgct cacctgcatc cgccgcgcct tcgatctcat agagaagcac gagggcaaga
      601 gcctgacgct cgcctcgctg ccgcgcgagg acgcagccgt ctacgacatg ctgtgcagag
      661 cggattcgat cggcgtgttc caggtggaaa gccgcgcgca gatgaacatg ctgccgcgcc
      721 tgaagccgcg ctgcttctac gacctcgtca tcgaggtggc gatcgtgcgc cccgggccca
      781 tccagggcga catggtgcac ccttacctgc gccggcgctc cggcgtggag cccgagcatt
      841 acccgtcgcc ttcgcccgca catgggccca agaacgagct ggagaacatc ctcggcaaga
      901 ccaagggcgt gccgctgttc caggagcagg cgatgcgcat cgcgctcgtc gcggcgaagt
      961 tctccgacgc cgaggtcaac gagctgagga aggcgatggc gaccttccgc cgccgcggca
     1021 ccatcgcgct cctggaggag aagatggtct cgcgcatggt ctcgcgcggc tacccgcccg
     1081 actttgcgca gcgctgcttc gaccagatca agggtttcgg cgaatacggc tttcccgaga
     1141 gccacgcggc gagctttgcg catctcgtct atgtttccgc ctggatcaaa tgccactacc
     1201 cagcggcctt tgccgccgcg ctgctcaact cccagccaat gggcttctac gcaccggcgc
     1261 agatcgtgcg cgatgcgcgc gagcacgagg tcgaggcgcg ctcagccgat gtgaacctct
     1321 ccgattggga ttgcacgctg gagaacatgg agggcgccgg cccggcgctg cggcttgggc
     1381 tgcgccagat cgacggcgca caggaggagg agatcaagaa gcttatggcc gcgcgtccct
     1441 tccgcgacgt gcacgatcta tggctgcgat caggcgtgaa gctcgtcacg ctggagcggc
     1501 tcgccgccgc cgatgctttc cgctcgctcg gcctcgaccg gcgccaggcg ctgtgggagg
     1561 tgaagggcct gagctcggcc gtgccgctac cgctcttttc ctggagcgag acgggcgaag
     1621 ccggcttgga gcccgccgtg gcgct
//
LOCUS       DASVII010000340         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_698684,
            whole genome shotgun sequence.
ACCESSION   DASVII010000340 DASVII010000000
VERSION     DASVII010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698684"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VF309_04475"
     CDS             <1..453
                     /locus_tag="VF309_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7465875.1"
                     /translation="DDDAMDYVVGVTLLNDLTDRDMQKREVASGSRFWTSKNAPGFGP
                     LGPEIVTMDEVGDPYDLWITCSVNGEERMRVNTGDQIWKMPDILEHFSRTIPIEPGDM
                     FSTGAPGGVAVGKANAKDLFLQPGDVVECSIEGITTLRTTIVSPSSLQ"
     gene            450..1397
                     /locus_tag="VF309_04480"
     CDS             450..1397
                     /locus_tag="VF309_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7465876.1"
                     /translation="MNVARLLARAVALAAFAFASQGALAQPFPAKPVRIIVPFPPGGG
                     ADALTRIMQPALTKMWAQPIIIENKAGASGHIGADFVAAADADGYTLLMSSTASLTEK
                     NVASFAPVTLVSASPYVVVVNPKVPARSVKELVEYAKKNPGKLAFGSSGTGAASHLAA
                     ELFKSLAGIDMLHVPYKGTGQAVTDLLAGQIDLMFAPAQSVMPHVQAGKLRALAVTSA
                     KRYSIIPDLPTVAEAGVPGYEAVGWFGLLAPVRTPAAIVGKLSADANKALGEPEVKQQ
                     MMILGAEPSGGTPAQFARFIHDDQAKWSKLMRERGIVPE"
BASE COUNT          275 a          572 c          565 g          263 t
ORIGIN      
        1 gacgacgacg cgatggatta cgtggtgggc gtgacgctcc tgaacgacct caccgaccgc
       61 gacatgcaga agcgcgaggt ggcctcgggc tcgcgcttct ggacttcgaa gaacgcgccg
      121 ggcttcggcc cgttgggtcc ggagatcgtc accatggacg aggtgggcga tccctacgac
      181 ctgtggatca cgtgctccgt gaacggcgag gagcgcatgc gcgtcaacac cggcgaccag
      241 atctggaaga tgcccgacat cctcgagcat ttctcgcgca ccattccgat cgagccgggc
      301 gacatgttct cgacgggcgc cccgggcggc gtggcggtcg gcaaggccaa cgcgaaggat
      361 ctcttcctcc agcccggcga cgtcgtcgaa tgctccatcg agggaatcac cacgcttcgc
      421 accaccatcg tctctccgtc cagcctgcaa tgaacgtcgc ccgcctcctc gcgcgcgccg
      481 tggcgctcgc ggccttcgcc ttcgcatcgc aaggcgccct cgcgcagccc ttccccgcca
      541 agcccgtgcg gatcatcgtg ccgttccctc cgggcggcgg cgccgatgcg ttgacccgca
      601 tcatgcagcc cgcgctcacg aagatgtggg cgcagcccat catcatcgag aacaaggccg
      661 gcgcgagcgg ccatatcggc gcggatttcg tcgccgcagc ggatgccgac ggctatacgc
      721 tgctcatgag ctccacggcc tcgctcaccg agaagaacgt ggcgagcttc gctcccgtga
      781 cgctcgtgtc ggcttcgccg tacgtcgtcg tcgtgaatcc gaaggtgccc gcgcgcagcg
      841 tgaaggagct cgtcgagtac gcgaagaaga atccgggcaa gctcgcgttc ggctcttccg
      901 gcaccggcgc cgcgtcgcat cttgccgcgg agctcttcaa gtcgctcgcg ggcatcgaca
      961 tgctgcacgt cccgtacaag gggacggggc aggcggtgac cgaccttctc gcggggcaga
     1021 tcgacctcat gttcgcgccc gcgcagagcg tgatgccgca cgtgcaggcg ggcaagctga
     1081 gggcgctcgc ggtgacgagc gcgaagcgct attcgatcat tcccgacctg ccgacggtcg
     1141 cggaggcggg cgttcccgga tacgaggcgg tgggctggtt cggcctgctc gcgcccgtga
     1201 ggacgccggc ggcgatcgtc gggaagctct ccgcggacgc gaacaaggcg ctcggcgagc
     1261 cggaagtgaa gcagcagatg atgatcctcg gcgcggagcc ctcgggcggc acgcccgcgc
     1321 agttcgcgcg cttcatccac gacgaccagg cgaagtggtc gaagctcatg agggagcgcg
     1381 gcatcgtgcc cgaatgacgg gtggcgggtg tcagcgctcg ccgaccccgg atcgcggcaa
     1441 gcgctggcag cgacgcgaca gcttccctcg taaccttccc caatgacccg cgcgaacgcg
     1501 cgaaacgttt cggaaggatc gaacatgaag atgcatgcac tgatttccgc gagtgtcgcc
     1561 ggcgtcgcgc tcgcgaccgc ggccgttccc gcactcgcct ggaccgtgtg gcccgatgtc
     1621 gacttcgagt ggtacgcgaa tgtgggccgc aacatgccgg gccccgccgt ggaga
//
LOCUS       DASVII010000341         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_267258,
            whole genome shotgun sequence.
ACCESSION   DASVII010000341 DASVII010000000
VERSION     DASVII010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267258"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="rfbD"
                     /locus_tag="VF309_04485"
     CDS             <1..718
                     /gene="rfbD"
                     /locus_tag="VF309_04485"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX7465877.1"
                     /translation="DRAESEEALARAVNAVAPGVIAEEAARAGALLVHFSTDYVFDGE
                     KSSPYVEDDPTHPLNAYGRTKLEGERNIAASGCAHVILRTSWVYGPQGSNFLFTMLRL
                     AATRDELRIVDDQRGAPTSSLQLARAVLALFTGAEPARPISAEDIARASGRRGLYHAT
                     AAGETTWFGFAKAIFEARARGANFKAPRLVAIATREYPTPARRPANSILSNERLERTF
                     GVRLGDWRAGLAETLSALPG"
     gene            complement(701..2359)
                     /locus_tag="VF309_04490"
     CDS             complement(701..2359)
                     /locus_tag="VF309_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7465878.1"
                     /translation="MLLSLHVALVAAPGSEFQRVWLLVHFGLFLLWQPFIAAERELEI
                     FSGVLLLAITGVTLYFLAGWMIVAWLLILLGILGGRVFVVQATQRNRFYLVAFAYVLA
                     ILLMRAVPALVLGEQSLPEPFSQIVSVLLPLALLLLAVLPVGSQENETGQVFDFFYAV
                     LVFQLGVVLVLGSIAFMRYTDDHYFASVALTVLAFGFALFVLAVLWNPLRGFGGLRTY
                     FSRYLLSVGMPFELWMRRIAELAETEPDSKRFLDEAFREIASLPWIKGAYWRSPDGEG
                     GYGETGGFATRFTYHQLDVMFHTEISLSPALSLHMRLLAQVVGEFYEGKRRENVLRHN
                     AYLQAVHETGARLTHDVKNLLQSLYALTSMAPREPEDGYGGLLQRQLPQLTKRLHATL
                     EKLRAPEVAIRELPVTSRAWWSDLERRLGDTGVVLKADVEMDVDVPAALFDSFIENAI
                     DNARAKMLREPGIELSVVFACDLQRVELSVCDTGTPVDAAVARKLFREPIERGGSLGI
                     GLFQAARQASLAAYRIELTHNRAGYVCFTLARADSPAADSPGKR"
     gene            2619..>2892
                     /locus_tag="VF309_04495"
     CDS             2619..>2892
                     /locus_tag="VF309_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7465879.1"
                     /translation="MKRQQSGFTLVEIAIVLVIIGLLLGGILKGQELINSAKVKNLAN
                     DFRVIPTYIYAYQDKFRALPGDDKGVVAHLGTTVPPVVPGDGNGVID"
BASE COUNT          535 a          960 c          995 g          402 t
ORIGIN      
        1 cgatcgcgcc gaatccgagg aagcgctcgc gcgcgccgtc aacgcggtcg cgcccggcgt
       61 gatcgcggag gaggccgcgc gtgccggcgc gctcctcgtc catttctcga ccgactacgt
      121 tttcgacggg gagaaatcgt ctccgtacgt cgaggacgat ccgacccatc cgttgaacgc
      181 ctacggccgc accaagctcg agggcgagcg caacatcgcg gcgagcggat gcgcgcacgt
      241 gatcctgcgc acgagctggg tctacggccc gcaaggctcg aactttctct tcacgatgct
      301 gcgtcttgcc gcgacgcgcg acgaattgcg catcgtcgac gaccagcgcg gggctccgac
      361 ctccagcctg cagctcgcgc gcgcggtgct ggcgctcttc accggggcgg agccggctcg
      421 ccccatcagc gccgaggaca tcgcgagagc ctccgggcgg cgaggcctgt atcacgccac
      481 ggcggcgggc gagacgacct ggttcggatt cgccaaggcc atcttcgaag cgcgcgcgcg
      541 cggcgcgaat ttcaaggccc cgcggctggt cgcgatcgcg acgcgcgaat acccgactcc
      601 cgcgcgccgg cccgcgaact cgatcctttc caacgagcgc ctcgagagga ctttcggcgt
      661 gcgcctcggc gattggcgcg cgggcctcgc ggaaacgctc tcagcgcttc ccgggtgagt
      721 ccgccgcggg ggaatccgcg cgagcgagcg tgaagcacac gtagcccgcg cggttgtgcg
      781 tgagctcgat gcgataggcc gcgaggctcg cctgccgcgc cgcctggaag agcccgatgc
      841 cgaggcttcc gccgcgctcg atcggctcgc gaaagagctt gcgcgccacc gcggcgtcca
      901 cgggcgtccc ggtgtcgcac accgacagct cgactcgctg caggtcgcac gcgaagacca
      961 cggacagctc gattcccggc tcgcgcagca tcttcgcgcg cgcgttgtcg atcgcgttct
     1021 cgatgaagct gtcgaagagc gcggcgggca cgtcgacgtc catctcgacg tcggccttga
     1081 ggacgacgcc cgtgtcgccc aggcgccgct cgagatcgga ccaccaggcg cgcgaagtca
     1141 ccggcagctc gcggatcgcg acctcggggg cgcgcagctt ctcgagcgtc gcgtgcaggc
     1201 gcttggtgag ctgcggcagc tggcgttgca gcaggccccc gtagccgtcc tcgggctccc
     1261 gcggcgccat cgaggtgagc gcgtagagcg actgcagcag gttcttcacg tcgtgcgtga
     1321 gccgcgcgcc cgtctcgtgc accgcctgca ggtatgcgtt gtggcgcagc acgttctcgc
     1381 ggcgcttgcc ctcgtagaac tcgcccacga cctgcgcgag gaggcgcatg tgcagggaaa
     1441 gcgcgggcga gaggctgatc tcggtgtgga acatcacgtc gagctggtga taggtgaagc
     1501 gcgtcgcgaa gccgcccgtt tccccgtagc cgccctcgcc gtcgggagag cgccaatacg
     1561 cgcccttgat ccagggaagc gaggcgattt ctcgaaacgc ctcgtcgagg aagcgcttcg
     1621 aatcgggctc ggtctcggcg agctccgcga tacggcgcat ccacagctcg aagggcatgc
     1681 cgaccgagag gaggtagcgc gagaaatacg tgcgcaggcc gccgaaccct cgcagcggat
     1741 tccacaggac ggcgagcacg aaaagcgcga atccgaaggc gagcaccgtg agcgcgaccg
     1801 aggcgaagta gtggtcgtcg gtgtagcgca tgaacgcgat cgagcccagc acgagcacga
     1861 cgcccagctg gaacacgagc acggcgtaga agaagtcgaa cacctggccg gtctcgttct
     1921 cctgcgagcc cacgggaagg accgcgagga ggagcagcgc gagcggcagc agcacggaga
     1981 cgatctgcga gaagggctcg ggcaggctct gctcgccgag caccagcgcg ggcaccgcgc
     2041 gcatcaggag aatcgcgaga acgtatgcga atgcgacgag gtagaagcgg ttgcgttgcg
     2101 tcgcctggac cacgaagacg cgcccgccga ggatgccgag gaggatgagg agccacgcga
     2161 cgatcatcca gccggcgagg aagtaaagcg tgaccccggt gatcgcgagg agtaggacgc
     2221 cggagaagat ctcgagctcg cgctcggcgg cgatgaaggg ctgccacagc aggaacagcc
     2281 cgaagtgcac gaggagccac acccgctgga attcgctccc gggcgcggcg acgagcgcca
     2341 catggagcga gagcagcatg gcaagcacca cccagcgccg ctgggaggcg atgcgggcag
     2401 cgaggcgctg ggcggtcgtg gtcgcagatg ccatggatgc gtcggaaagc cgacacgcgc
     2461 tgtcgggacg gcggacagat accggggtcc gcgcctcgtc gaaagtccag cgaaagccga
     2521 ataatactga aaaacagaat cttactgcgg gtggcatggg gcttgcacta ctctacccat
     2581 atcgcttgct tcacaccgct tcgaaacagg agacacaaat gaaaaggcaa caatccggat
     2641 tcaccctcgt cgagatcgcg atcgtgctcg tgatcatcgg cctgctgctg ggcgggatcc
     2701 tcaagggcca ggaactcatc aacagcgcca aggtgaagaa cctcgccaac gacttccgcg
     2761 tgattcccac ctacatctac gcctaccagg acaagttcag ggcgcttccc ggcgacgaca
     2821 agggcgtcgt ggcgcacctc ggcaccaccg tgcccccggt cgtcccgggc gacggcaacg
     2881 gcgtcatcga cg
//
LOCUS       DASVII010000342         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_303288,
            whole genome shotgun sequence.
ACCESSION   DASVII010000342 DASVII010000000
VERSION     DASVII010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303288"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VF309_04500"
     CDS             complement(<1..1270)
                     /locus_tag="VF309_04500"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX7465880.1"
                     /translation="MGSLFASDALLASGWARDVLVEWDAAGIISRVQPGSEPGKHARA
                     AGPVLPGIPNVHSHAFQRAMAGLAEFRGHPTDDFWTWREEMYRLVTRLEPEDVEAIAR
                     HLYIEMLRHGYTTVAEFHYLHNDRDGRHYEDRPEMANRIVSAAEDSGIALTLLPVLYA
                     HGGFGHKVLSSAQRRFESDPAFVIELLRELAAFHLPNPRLRLGVAPHSARAVDALMLT
                     ELVDAARRFDGTMPMHMHVSEQVGEVTECIETHGTTPLAWINDLVDVDDRWCFIHSTH
                     LTRIETRALGATGACVGLCPMTEANLGDGVFEFVPWFEARGPWSIGGDSHVSVSPFEE
                     LRALEYSQRLRHRIRNVIADEESPDVAANLWRGAASGGAQATGQPAGSIAAGRRADLV
                     VLDGSDLDFEGLDASASLGVALFSGNSNRVR"
     gene            1292..>2304
                     /gene="hutI"
                     /locus_tag="VF309_04505"
     CDS             1292..>2304
                     /gene="hutI"
                     /locus_tag="VF309_04505"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX7465881.1"
                     /translation="MTRTLLVNARIATMRGGRYSIIDDAAMRMQDGRIEWIGPARSLA
                     PRPGGGGEEAFDAQGALVTPGLVDCHTHLVYAGDRAADFEMRLAGASYADIARAGGGI
                     VSTVKATRAASNAELRAVSAKRLEALMAEGVTTVEIKSGYGLDVENELRCLRIARSLA
                     ADHGVSICTTLLGAHALPPEFAGRADDYIDAVCTQMIPRVAAEGLASAVDAFCETIAF
                     NSAQVRRVFEAARAHGLAVKLHADQLSDSGGASLAAQFGALSADHLEHAGEAGVAALA
                     AAATVAVLLPGAYYFLRETKAPPVELLRKHGVPMAIATDCNPGTSPSTSLPTMMNMAC
                     TLFR"
BASE COUNT          366 a          778 c          820 g          340 t
ORIGIN      
        1 cgcgcacccg gttcgagttg ccggaaaaga gagcgacccc gagcgaggcc gaggcgtcga
       61 gcccctcgaa gtcgagatcg gagccgtcga gcaccacgag atccgcgcgc cgtcccgcgg
      121 cgatcgatcc cgcgggctgc ccggtcgcct gcgcgccgcc gcttgccgcg ccgcgccaga
      181 ggttcgccgc gacatcgggc gattcctcgt ccgcgatcac gttgcgaatg cgatggcgca
      241 ggcgctggct gtactcgagc gcgcgcaact cctcgaaggg actcaccgag acgtggctgt
      301 cgccgccgat cgaccacggc ccgcgcgcct cgaaccaagg cacgaactcg aagacgccgt
      361 cgccgaggtt ggcttccgtc atcgggcaga ggcccacgca ggcgccggtc gcgcccaggg
      421 cgcgcgtctc gatccgcgtg aggtgcgtcg agtggatgaa gcaccaccgg tcgtcgacgt
      481 ccacgaggtc gttgatccag gcgagcggcg tcgtgccgtg ggtttcgatg cattcggtca
      541 cttcgccgac ctgctccgag acgtgcatgt gcatcggcat cgtcccatcg aagcgccgcg
      601 ccgcatcgac cagctcggtg agcatgagcg catcaacggc gcgcgccgag tgaggcgcca
      661 cgcccagccg aagccgcgga ttcggaaggt gaaaggcggc gagctcgcgc aggagctcga
      721 tcacgaacgc ggggtcgctt tcgaaacggc gctgggcgct cgaaagcacc ttgtggccga
      781 agccgccgtg ggcgtacagc accggcagca gcgtgagcgc gatgccggaa tcctccgccg
      841 ccgaaacgat gcggttcgcc atctcggggc ggtcttcgta gtggcgcccg tcgcgatcgt
      901 tgtgcaggta gtggaattcg gcgaccgtcg tatacccgtg acgcagcatc tcgatgtaga
      961 gatgcctcgc gatggcctcg acgtcctcgg gctcgaggcg cgtgacgagg cggtacatct
     1021 cctcgcgcca cgtccagaaa tcgtcggtcg gatggccgcg gaattccgcg agtcccgcca
     1081 tggcccgctg gaacgcatgg gaatgcacat tggggatgcc gggaaggacg gggcccgcgg
     1141 cgcgcgcgtg cttgccgggt tcgctgccgg gctggacccg cgagatgatt ccggccgcgt
     1201 cccattcgac aaggacgtcg cgcgcccagc cggaagcgag cagcgcgtcg gaggcgaaga
     1261 gtgagcccat gaagttctct atgataccgg catgactcga accctcctcg tgaacgcgcg
     1321 gatcgcgacg atgcgcggcg gacgctattc gatcatcgac gacgcggcga tgcgcatgca
     1381 ggacgggcgt atcgagtgga tcggaccggc gcgaagcctc gctccgcggc ccggcggcgg
     1441 cggcgaagag gcgttcgacg cacaaggggc gctcgtgacc ccgggtctcg tcgattgcca
     1501 cacgcacctt gtctacgcgg gcgaccgcgc cgcggacttc gagatgcggc ttgcgggggc
     1561 gagctatgcg gacatcgcga gggcgggcgg cggaatcgtc tccacggtga aggccacgcg
     1621 cgcggcgagc aacgcggagc ttcgcgccgt ctcggcgaag cgcctcgagg cgctcatggc
     1681 cgagggggtg acgacggtcg agatcaagtc gggatacggg ctggatgtcg agaacgagct
     1741 gcgctgcctg cggatcgcga ggagcctcgc cgcggaccac ggtgtgtcga tctgcacgac
     1801 gctcctcgga gcgcacgcgc tgccgccgga attcgccggc cgggcggacg actacatcga
     1861 cgcggtgtgc acgcagatga tcccgcgcgt cgcggccgaa gggctcgcga gcgcggtcga
     1921 tgcgttctgc gagacgatcg cgttcaacag tgcccaggtg cgccgcgttt tcgaagcggc
     1981 gcgcgcccac ggcctcgccg tgaagctgca cgccgaccag ctctccgatt ccggcggcgc
     2041 ctcgctcgcg gcgcaattcg gcgcgctttc cgcggaccac ctcgagcatg cgggagaggc
     2101 aggggtcgcc gcgctcgccg ccgcggcaac ggtcgccgtt ctattgccgg gcgcctacta
     2161 cttcctgcgc gaaacgaagg cgcccccggt ggagctcctg cgcaagcacg gcgtgccgat
     2221 ggcgatcgcg accgattgca atccggggac ctcgccttcg acctcgcttc ccaccatgat
     2281 gaacatggcc tgcaccttgt tccg
//
LOCUS       DASVII010000343         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_16182, whole
            genome shotgun sequence.
ACCESSION   DASVII010000343 DASVII010000000
VERSION     DASVII010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16182"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VF309_04510"
     CDS             complement(<1..869)
                     /locus_tag="VF309_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEX7465882.1"
                     /translation="MTNLPATGTSSRSAQGAKGAAAKKPAKEVDTGSSLANFRAPFIG
                     GKPVTTQMQILGTAATVLLVLTGALVYQDTTARTRNATFISIASQMQFHTQRLAKAAG
                     LAARGQQAAFPQVEDSRAKFEEYLKVLRNGGVALGVSLPSAESSAELTSRIEDLTKRW
                     PESSSSAGAILAARNDLVALAQNVAQVRVGAEEMANQSQELTGLMQQSGMAPGQVLRA
                     NRITFLSERLGRGAAEILGSEVIDPEVPFLIGKDTNDLRGLILALEVGNEQMGMNALR
                     DAEARAKLAELKKT"
     gene            complement(1000..1512)
                     /locus_tag="VF309_04515"
     CDS             complement(1000..1512)
                     /locus_tag="VF309_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7465883.1"
                     /translation="MSRRTGLREFQLSVAERIRTASTRTALSSKLGFQVGAESWFVAL
                     NQVNEVIPVPPTVAVPLTQSWFRGLANIRGNLYSVLDFSAFKGGDPTPAGVERRVILI
                     SDRLVGGAGLLVSRMLGLRNPEQFTAAERPADAPPWVAGAYTDAGGTRWLELDFPALV
                     REQRFLEVGA"
     gene            complement(1517..1882)
                     /locus_tag="VF309_04520"
     CDS             complement(1517..1882)
                     /locus_tag="VF309_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465884.1"
                     /translation="MAIKKIMVVDDSPTERAFMEGVLKKRGYEVLLVDSGEAAIERSK
                     SDLPDLILMDVVMPGLNGFQATRAITREEKTKHIPVIICTTKDQETDKIWGLRQGAKD
                     YVTKPIGESDLLEKIKALG"
     gene            complement(1918..2283)
                     /locus_tag="VF309_04525"
     CDS             complement(1918..2283)
                     /locus_tag="VF309_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465885.1"
                     /translation="MVIDDSNTIRRSAEIFLMQAGCQVILAENGFDALAKIADHQPDL
                     IFVDIMMPRLDGYQTCALIKKSGKHGATPVIMLSSKDSLFDRARGRMVGSDEYLTKPF
                     TKESLLRAVESHLAQRSTA"
     gene            complement(2563..2757)
                     /locus_tag="VF309_04530"
     CDS             complement(2563..2757)
                     /locus_tag="VF309_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX7465886.1"
                     /translation="MSSPAPESQPFRMWRCLACGLLYDEAEGWPDDGIAPGTRWEDIP
                     AGWCCPECGARKDDFEMVEL"
     gene            2893..>3523
                     /locus_tag="VF309_04535"
     CDS             2893..>3523
                     /locus_tag="VF309_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEX7465887.1"
                     /translation="MNESVLSLPAVLTFAATDPTSGAGLQADLLALASMGCYPLSVVT
                     ALTVQDTAGVESFYAIDPDWVADQARCILEDMPVAAFKMGMLGSTEIVTVVAEVVSDY
                     PDVPLVLDPVMGSGRGDEFAGDDMVSAIRELLVPQSTVVTPNIPELRRLAGEDDNDDV
                     SLADCAQILLDSGCEYVLVTGTHDSTADVVNTLYHRGGVLRADTWQRLPG"
BASE COUNT          563 a         1165 c         1133 g          662 t
ORIGIN      
        1 gtcttcttca gctcggccag cttggcgcgc gcctcggcgt cgcgcagcgc gttcatgccc
       61 atctgctcgt tgccgacctc gagcgccagg atcaggccgc gcaggtcgtt ggtgtccttg
      121 ccgatgagga acggcacctc cggatcgatg acctcggagc cgagaatctc cgcggcgccg
      181 cgcccgagac gctccgaaag gaacgtgatg cggttcgcgc gcagcacctg gcccggcgcc
      241 atgcccgact gttgcatgag gccggtgagc tcctgcgact ggttcgccat ctcctccgcg
      301 ccaactctca cctgcgcgac gttctgcgcg agggccacga ggtcgttgcg cgcggcgagg
      361 atcgcgccgg cggagctcga gctctcgggc cagcgcttcg tgagatcctc gatgcggctc
      421 gtgagctcgg cactcgattc cgcgctcggc aggctgacgc cgagggccac gccgccgttg
      481 cgcagcacct tgaggtattc ctcgaacttc gcgcggctgt cctccacctg gggaaacgcg
      541 gcctgctgcc cgcgcgcggc aagtcccgcc gccttggcga gacgctgcgt gtggaactgc
      601 atctgcgaag cgatcgagat gaaggtggcg tttcgcgtgc gcgccgtcgt gtcctggtag
      661 acgagagccc ccgtgaggac cagcagcacc gtggccgccg ttccgaggat ctgcatctgc
      721 gtcgtgaccg gcttgccgcc gatgaagggc gcccggaagt tcgcgagcga ggagccggta
      781 tcgacttcct tggccggctt cttcgccgcc gcgcccttgg cgccttgcgc cgatcgcgac
      841 gaggttcccg tcgccgggag gttggtcatg gtcgatgcct gtgccgcggc gcccgggcgc
      901 gtcgccggtt tctgcttcga gccagtgaat cgcctgaacc agtctccgaa cgaggatttc
      961 ggtttgatcg tctgtgcttt agccattggc ctttttctcc tacgcgccta cttccaagaa
     1021 ccgctgctcg cgaacgaggg cgggaaaatc gagctcgagc cagcgggtac cgccggcatc
     1081 cgtatatgcg cctgcgaccc atgggggcgc gtcggctggc cgctccgcgg ccgtgaactg
     1141 ttccggattg cgaagtccca gcatgcgtga aaccagcaag cccgcgcccc cgacgaggcg
     1201 atcggagatg aggatcacgc ggcgctcgac gccggcgggc gtcggatcgc cgcccttgaa
     1261 ggcggagaag tcgagcacgc tgtacaggtt gccgcgaatg ttggccaggc cgcggaacca
     1321 cgactgggtc aggggcacgg ccaccgtcgg cggaaccgga atcacttcgt tcacctggtt
     1381 cagggccacg aaccagctct cggccccgac ctggaagccc agcttcgacg agagcgccgt
     1441 gcgcgtcgag gccgtgcgaa tccgctccgc gaccgaaagc tggaattccc gaagtcccgt
     1501 gcggcgtgac atgtcgctag cccagcgcct tgatcttctc gagcaggtcg ctctcgccga
     1561 tcggcttggt gacgtagtcc ttcgcgccct ggcgaaggcc ccagatcttg tcggtttcct
     1621 ggtcctttgt cgtacagatg atcaccggga tgtgcttggt cttttcctcg cgcgtgatcg
     1681 cgcgagtggc ctggaagccg ttgaggcccg gcatcacgac gtccatcagg atgaggtcgg
     1741 gcaggtcgct cttggagcgc tcgatcgccg cctcgccgct atccacgagc aggacctcgt
     1801 atccgcgctt cttcagcacg ccctccatga aggcgcgctc cgtcggcgaa tcgtccacca
     1861 ccattatctt cttgattgcc attgagatct cttcgcagta tttggagctg attgtcgtca
     1921 ggcggtgctg cgctgtgcga gatgcgattc gacggcacgc aacaggcttt ccttggtgaa
     1981 cggcttcgtc aggtactcgt ccgagcccac catgcggcca cgcgcgcggt cgaagaggct
     2041 gtccttcgac gagagcatga tcaccggcgt cgcgccatgc ttgccgctct tcttgatgag
     2101 cgcgcacgtc tggtagccat cgaggcgcgg catcatgatg tcgacgaaga tgagatcggg
     2161 ctggtgatcg gcgatcttcg ccagcgcgtc gaaaccgttc tcggcgagga tgacctggca
     2221 gccggcctgc atgagaaata tctcagcgct gcgccgaatg gtattgctgt catcgatgac
     2281 catcacgcgt acacccgtga ggttctttga gtccgccaca cttccctcga ttcatgttcg
     2341 gctgcgtcgg gaaaatgggc ggccggaatt accggcagtc ccgggccgct cgcggtccgc
     2401 atcccctgaa agccccgcag ccgcgttcta cgcgatcttc agattattcc atttgccgat
     2461 gcaaaacaac tttttaccaa ccaaaacaac tgctaaccca gagcttctca tctattttgg
     2521 aagtttagag ggaagcgtcc cgggaacgcg gctccgaacg ggtcagagtt cgaccatttc
     2581 gaagtcgtcc ttgcgcgcgc cgcactcggg gcagcaccag cccgccggaa tgtcctccca
     2641 gcgggttccc ggcgcgatgc catcgtcggg ccagccctcg gcctcgtcgt agagcaggcc
     2701 gcaggcgagg caacgccaca ttctgaaagg ttgcgactct ggcgcgggag aagacatagg
     2761 cgaacgcgtt gacggttgag tagaatgtga ggacgcgcgc gctcacttag aagcatcgta
     2821 gcgatccccg gtcggcgcgc gcaacgcgag ccgggtttca agtgtaaccc cccctccacc
     2881 gcccgcgcgc ccatgaacga atccgtgcta tcgctccccg ccgtcttgac gttcgccgcc
     2941 accgatccca cgagcggcgc ggggctccaa gcggatctcc tcgcgctggc gagcatgggt
     3001 tgctatcccc tttcggtggt cacggcgctc acggtgcagg acaccgcggg cgtcgagtcc
     3061 ttctacgcca tcgaccccga ttgggtggca gaccaggcgc gctgcatcct cgaggacatg
     3121 cccgtcgcgg cgttcaagat ggggatgctc ggctccaccg agatcgtgac cgtggtggcg
     3181 gaggtcgttt cggactatcc cgacgtgccg ctcgtgctgg atcccgtgat gggttcgggc
     3241 cgcggcgacg agttcgcggg cgacgacatg gtttccgcga tccgcgagct gctcgtgcca
     3301 caaagcaccg tcgtcacgcc caacatcccg gagcttcgcc gcctggcggg cgaggacgac
     3361 aacgacgacg tgagcctcgc ggattgcgcg cagatcctgc tcgacagcgg ctgcgagtat
     3421 gtcctggtga cgggcaccca cgattcgacc gcagacgtcg tgaacacgct ctatcaccgc
     3481 ggcggcgtgc tgcgcgcgga tacgtggcag cggcttccgg gca
//
LOCUS       DASVII010000344         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_339693,
            whole genome shotgun sequence.
ACCESSION   DASVII010000344 DASVII010000000
VERSION     DASVII010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339693"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..763)
                     /locus_tag="VF309_04540"
     CDS             complement(44..763)
                     /locus_tag="VF309_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7465888.1"
                     /translation="MVDTKKSKLLAGASGYSFKEWKGTFYPEKMKPEEMLAYYSQRLP
                     TVEINNTFYRMPKASVLESWAATAPEGFRFGIKASRRITHMSRIKAESSAEPLGWLYT
                     NLAALGPKRGPVLFQLPPNMKKDLPRLAEFLALLPADHRAAFEFRNDTWFGDDVYDAL
                     KGAGAALVLSERQDNAPPPLVETAPWGYVRLRLETYSEDDLKAWAERLAATGWRETFV
                     YFMHEPTAPAYAASLMRYQAG"
     gene            753..>1510
                     /locus_tag="VF309_04545"
     CDS             753..>1510
                     /locus_tag="VF309_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7465889.1"
                     /translation="MSTTAFPYNSRMAKDSLAEYSSKRAFASTPEPAADLPKARSGPL
                     LFVVQLHAAQRLHFDLRLECDGVLKSWAVPKGPSLDPGEKRFAALTEDHPYEYASFEG
                     VIPPKQYGAGEMIVWDCGVYAPDEEGPARFDDREAAEREVREGFEKGKIGFELRGEKL
                     KGSFALVRMKDKKEWLLIKHKDRFVSSDDVTAKDRSVLLGAAVEDLKVVPAHRIAAST
                     LVPSGKIEAMPAKLAPMLSEVGDTAFNRGDWMWEP"
BASE COUNT          247 a          503 c          507 g          253 t
ORIGIN      
        1 gagaagcatt ggccggcggc aagcctggcg cggcgcaagg cgatcatccc gcctggtagc
       61 gcatgagcga cgcggcgtac gcgggcgccg tgggctcgtg catgaaatac acgaaggtct
      121 cgcgccagcc ggtcgcggcg aggcgctccg cccacgcctt caggtcgtct tcgctgtagg
      181 tctcgagccg caggcgcacg tagccccacg gcgcggtttc caccaggggc ggcggcgcgt
      241 tgtcctggcg ctcggagagc accagcgccg cacccgcgcc tttcaacgcg tcgtacacgt
      301 cgtccccgaa ccaggtgtcg ttgcgaaact cgaaggcggc gcggtgatcg gcgggcagga
      361 gcgcgaggaa ttccgcgagg cgcggcaggt ccttcttcat gttgggcggc agctggaaga
      421 gcacgggccc gcgcttcggt cccagcgccg cgaggttggt gtagagccag cccagcggtt
      481 ccgccgacga ttccgccttg atgcgcgaca tgtgcgtgat gcgccgcgac gccttgatcc
      541 cgaagcgaaa gccctcgggc gcggtcgcgg cccagctctc cagcaccgag gccttcggca
      601 tccggtagaa ggtgttgttg atctccaccg tcggcagccg ctgggagtaa taggcgagca
      661 tctcctcggg cttcatcttc tccgggtaga acgtgccttt ccattccttg aaggaatagc
      721 cgctcgcgcc cgccagcaat ttcgattttt tcgtgtccac cacggccttt ccctacaatt
      781 cccgcatggc gaaagactcc ctggccgaat attcctcgaa gcgcgccttc gcgtccacgc
      841 ccgagcccgc ggccgacctg cccaaggcac gcagcggccc gctcctcttc gtggtgcagc
      901 tgcatgccgc ccagcgcctg cacttcgacc tgcgcctcga gtgcgatggc gtcctcaagt
      961 cgtgggccgt gcccaagggg ccgtcgctcg atcccggcga gaaacgcttc gcggcgctca
     1021 ccgaggacca tccttacgag tacgcgtcgt tcgagggcgt gatcccgccg aaacagtatg
     1081 gcgcgggcga gatgatcgtg tgggactgcg gcgtgtatgc gcccgacgag gaggggccgg
     1141 cgcgattcga cgaccgcgag gccgccgagc gcgaggttcg tgaaggattc gagaaaggca
     1201 agatcggctt cgagctgcgc ggcgagaagc tgaagggctc cttcgcgctg gtgcgcatga
     1261 aggacaagaa ggagtggctc ctcatcaagc acaaggaccg cttcgtatcc tccgacgacg
     1321 tcaccgcgaa ggaccgctcg gtcctcctcg gcgccgcggt cgaggatctc aaggtcgttc
     1381 ccgcgcatcg catcgccgcg tcgacgctgg tgccttccgg aaagatcgaa gcgatgcccg
     1441 cgaagctcgc accgatgctc tcggaggtgg gcgacacggc tttcaaccgc ggcgactgga
     1501 tgtgggagcc
//
LOCUS       DASVII010000345         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_375923,
            whole genome shotgun sequence.
ACCESSION   DASVII010000345 DASVII010000000
VERSION     DASVII010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375923"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VF309_04550"
     CDS             <1..238
                     /locus_tag="VF309_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7465890.1"
                     /translation="AMQSLDDPEDRAAGLLALRTVAEAYGGLAAVAADAGISREALYR
                     SLSPKGNPTLKTLLAVLNVVGMRLSVEPGERAAA"
     gene            339..914
                     /locus_tag="VF309_04555"
     CDS             339..914
                     /locus_tag="VF309_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX7465891.1"
                     /translation="MFQVRIHGRGGQGVVSAAEMLSVAAFEEGRHAQAFPSFGSERMG
                     APVMAFCRIDSREIRLREPVMQPDALIIQDPTLLHQVDLFSGLAPTGYILINSTRGFE
                     ELGIAEFTKKFPPSHLCVVPASDLAMKHVGRPLPNAALLGAFAAITGEVSFDAIANAI
                     RRKFSGKVGEGNVAAARAAYDLASKKEAALA"
     gene            907..>1678
                     /gene="porA"
                     /locus_tag="VF309_04560"
     CDS             907..>1678
                     /gene="porA"
                     /locus_tag="VF309_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEX7465892.1"
                     /translation="MLKQIEGSQAIAQAIALCRPEVICAYPITPQTHIVEGLGEMVKN
                     GSLEKCEFINVESEFAALSVAIGSSAAGARSYTATASQGILYMAEAVYNAAGLGLPIV
                     MTIGNRAIGAPINIWNDHSDSMALRDAGWIQLYAETNQEAADLHIQAFRLAEELSCPV
                     MVCVDGFILTHAYERIDIPTQKQVDDYLPPFVPVQILDVDDPVSMGAMVGPEAFMEVR
                     YLQHHKQQRALALIPTLAKEFREQFGRDSGGLLRTYHCE"
BASE COUNT          290 a          563 c          542 g          283 t
ORIGIN      
        1 ggcgatgcaa tccctggacg atcccgaaga tcgggcggca ggtcttctcg ctcttcgcac
       61 tgtcgcggag gcatacggcg ggctcgcagc ggtcgctgca gatgcgggca tcagccggga
      121 ggcgctctat cgctcgctct cgccgaaggg caatcccacc cttaagactc tccttgccgt
      181 gctgaacgtg gtcggaatga ggctctcggt ggaacccggg gaacgagcgg ccgcgtagcc
      241 cttcgcgatt cgtcgatgcc gtgaccggtt gacttggatc aatcggtccc gagcccatgc
      301 gccgagaatc aatgcgagct acatcaaggg agctcgcgat gttccaggtg cgcatccacg
      361 gccgcggcgg ccagggcgtg gtctcggcgg cggagatgct gtcggtcgcc gctttcgaag
      421 agggtcgcca cgcgcaggcg ttcccgagct tcggctccga gcgcatgggc gcgcccgtca
      481 tggccttctg ccgcatcgac tcgcgcgaga ttcgcctgcg cgagccggtc atgcagcccg
      541 acgcgctgat catccaggac cccacgctcc tccaccaggt cgatctcttc agcggcctcg
      601 cgcccaccgg atacatcctc atcaactcga cgcgcgggtt cgaggagctc ggcatcgccg
      661 aattcacgaa gaagtttccg ccaagccacc tgtgcgtcgt gcccgcatcg gatctcgcga
      721 tgaagcacgt cggtcgtccg ctgcccaacg cggctctgtt gggcgcattc gccgcgatca
      781 cgggcgaggt gagtttcgat gcgatcgcca acgccatccg ccggaagttc agcggcaagg
      841 tgggcgaggg caacgtcgcc gcggcgcgcg ccgcctacga cctggcgagc aagaaggagg
      901 ccgcccttgc ttaagcagat cgagggatcg caagccatcg ctcaggccat cgcgctttgc
      961 cggccggaag tcatctgcgc ctatcccatc acgccgcaga cccacatcgt cgagggactc
     1021 ggcgagatgg tgaagaacgg ctcgctcgaa aagtgcgagt tcatcaacgt cgagagcgag
     1081 ttcgcggcgc tctcggtggc gatcggctcg tcggccgccg gggcgcgcag ctacaccgcg
     1141 accgcgagcc agggaatcct ctacatggcc gaggccgtgt acaacgccgc gggcctcggg
     1201 cttccgatcg tgatgaccat cggcaaccgc gcgatcggtg cgcccatcaa catctggaac
     1261 gatcactcgg acagcatggc gctgcgcgac gcgggctgga tccagctcta cgcggagacc
     1321 aaccaagaag cggccgatct ccatatccag gcgtttcgtc tcgccgagga gctctcgtgc
     1381 cccgtgatgg tgtgcgtcga cggcttcatc ctcacgcacg cgtatgagcg catcgacatc
     1441 ccgacgcaga agcaggtcga cgactacctg ccgccgtttg tgccggtgca gatcctcgac
     1501 gtggacgatc ccgtgtcgat gggcgcgatg gtcggccccg aagcgttcat ggaagtgcgc
     1561 tacctccagc atcacaagca gcagcgcgcg ctcgcgctca tcccgacgct cgcgaaggaa
     1621 ttccgcgagc agttcgggcg cgactcgggt gggctcctgc gaacctatca ttgcgagg
//
LOCUS       DASVII010000346         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_87746, whole
            genome shotgun sequence.
ACCESSION   DASVII010000346 DASVII010000000
VERSION     DASVII010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87746"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VF309_04565"
     CDS             <1..436
                     /locus_tag="VF309_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7465893.1"
                     /translation="IPYKGGAGPAVTGLLSGDTHFMFVTLSSAMAFIQAGRLKALGVT
                     TPKRIDSLPNVPTLVEAGYPDMVSSSWQGVFVPAGTPRAIVDKLHRALLATLGTPEMH
                     QRFAVGGAQVVTSAAPEEFASFVASEAVRWGKVARESGATVD"
     gene            449..>1708
                     /locus_tag="VF309_04570"
     CDS             449..>1708
                     /locus_tag="VF309_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7465894.1"
                     /translation="MANFGMVGLDWQQRVNWDRLRNYRLERARERMKAHGLGAMLLMY
                     DENVRYVTSTLTPGWNRLKPGLRYALLCGDDPPVLFEQGDIGAHIKRHSPWIPKENVR
                     YSYAWIKGAAGPASTQQVTKFTNAIVAEMKKSNVSGMKLGVDFIDINMIQIFKEAKID
                     WVDGMSPMMEARAVKNVDEQECMRMVGAIGDAAHWECMKFLRPGLTENQVTAHIMEFL
                     YNIPGMEDVEDVIVSSGLNTWPNWRNFSDRIIKPGDIVFMDLAALTWNGYKSCYYRTY
                     CVGKEPTNEQKEYYAIALKWLYDSIKAVKVGATTRDIALKWPSAKEAWGYKEEDQAAA
                     NLWGHGLGLAQYDPPVISRIWSLDHPIPIQEGMVFALETQHGKTHQWGVRIEEMLIVH
                     KDKIEIVSNFPVEQITVVDPMVGYSTYG"
BASE COUNT          341 a          530 c          567 g          270 t
ORIGIN      
        1 catcccctac aagggaggcg cgggccccgc ggtgacgggt ctcctctccg gcgacacgca
       61 cttcatgttc gtgacgcttt cctccgccat ggccttcatc caggcgggac ggctgaaggc
      121 gctcggcgtc acgacaccca agcgcatcga cagcctgccg aacgtgccga ccctggtcga
      181 ggcgggctat ccggacatgg tgagcagctc gtggcaaggc gtgttcgtgc ccgccgggac
      241 gccgcgcgcc atcgtcgaca agcttcacag ggcgttgctc gccacgctgg gcacgccgga
      301 gatgcaccag cgcttcgcgg tgggcggagc gcaggtggtc accagcgccg cgcccgaaga
      361 gttcgcgtca ttcgtcgcct ccgaagccgt tcgctggggc aaggtggcaa gggaaagcgg
      421 agcaaccgtc gattaactgg gagacaagat ggctaatttc ggcatggtgg gactggactg
      481 gcagcagcgc gtcaactggg accggctgcg caactaccgc ctggagcgcg cgcgggagcg
      541 gatgaaggcc cacggcctcg gcgcgatgct cctcatgtac gacgagaacg tgcgctacgt
      601 gacgagcacg ctcacgcccg gctggaaccg gctgaagccg gggcttcgct acgcgctcct
      661 ctgcggcgac gatccgccgg tcctcttcga gcagggcgac atcggcgcgc acatcaagcg
      721 ccactcgcca tggattccga aggagaacgt gcgctactcg tacgcgtgga tcaagggtgc
      781 cgcgggaccg gcctcgacgc agcaggtgac gaaattcacc aacgcgatcg tcgccgagat
      841 gaagaagagc aacgtctcgg gcatgaagct cggcgtcgac ttcatcgaca tcaacatgat
      901 ccagatcttc aaggaggcga agatcgactg ggtcgacggc atgtcgccga tgatggaggc
      961 gcgcgccgtc aagaacgtgg acgagcagga gtgcatgcgc atggtgggcg cgatcggcga
     1021 cgccgcgcac tgggaatgca tgaagttcct gaggcccggc ctcaccgaga accaggtgac
     1081 cgcgcacatc atggaattcc tctacaacat cccggggatg gaggacgtcg aggacgtgat
     1141 cgtctcctcg gggctcaaca cctggcccaa ctggcgcaac ttctccgatc ggatcatcaa
     1201 gccgggcgac atcgtgttca tggatctcgc cgcgctcacg tggaacggct acaagtcgtg
     1261 ctactaccgc acgtactgtg tcggcaagga gcccacgaac gagcagaagg agtactacgc
     1321 catcgcgttg aagtggctct acgattcgat caaggcggtc aaggtcggcg ccaccacgcg
     1381 cgacatcgcg ttgaagtggc cctcggcgaa ggaagcgtgg ggctacaagg aagaagacca
     1441 ggcggcggcc aacctgtggg gccatggcct gggcctcgcg cagtacgatc ccccggtgat
     1501 ctcgcgcatc tggtcgctcg accatccgat cccgatccag gaggggatgg tgttcgcgct
     1561 cgagacgcag cacggcaaga cgcaccagtg gggcgtgcgc atcgaggaga tgctgatcgt
     1621 gcacaaggac aagatcgaga tcgtctccaa cttcccggtg gagcagatca ccgtcgtcga
     1681 tcccatggtc ggatattcga cttacgga
//
LOCUS       DASVII010000347         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_195441,
            whole genome shotgun sequence.
ACCESSION   DASVII010000347 DASVII010000000
VERSION     DASVII010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195441"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1891
                     /locus_tag="VF309_04575"
     CDS             <1..>1891
                     /locus_tag="VF309_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7465895.1"
                     /translation="RFVAKLFAIEEEWRDLVESHHELAPLFRAKRKFVQRRAMLKIKA
                     DEAAALDGAALEAEVVALLGGAFDELVFAQNVLGWMAHEAEHAAELSVAERFAAWAAH
                     TEEGRRRFRDGVLFKPPQRVDPMHLVPVQTDASRGYSVHTLHHIRRREGFALTDPGTS
                     LAGALDEANYCIWCHEQAKDSCSKGLKEKPAADGSVAFKKSPFGVPLAGCPLEERISE
                     FHKLKTEGWAIGALAMIVIDNPTVCSTGHRICNDCMKSCIYQRQDPVDIPQAETRTLK
                     DVLELPWGFEIYSLLTRWNPLSLRRPLPAAATGKRVLVIGMGPAGFTLAHHLMNDGHA
                     VVGIDGLKIEPLEARLSGVEPDGTRVAFEPVRDLSELREPLERRVMAGFGGVAEYGIT
                     VRWDKNFLKMARLLVERRAQFAMFGGVRFGGTLTAQMAFEMGFDHIALAAGAGKPTVL
                     EMPHGLARGVRTASDFLMALQLTGAAREESIANLQIRLPIVVVGGGLTAIDTATESLA
                     YYPVQVEKFLARYETLVVETSVGSVRNEWNEEETEVAEEYLTHALAIRAERARAAAEG
                     REPHILELLQAWGGSTIAYRKRLIDSPSYTLNHEEVEKALEEGIRFAENLTPLRVEVD
                     EAGRAS"
BASE COUNT          305 a          652 c          648 g          286 t
ORIGIN      
        1 ccgcttcgtc gcgaagctct tcgcgatcga ggaggaatgg cgcgacctcg tcgagagcca
       61 ccacgagctg gctcccctct tccgcgcaaa gcgcaagttc gtgcagcgcc gcgcgatgct
      121 gaagatcaag gccgacgagg ccgcggcgct cgacggcgcg gcgctcgagg ccgaggtcgt
      181 cgcgctcctc ggcggcgcct tcgacgagct cgtgttcgcg cagaacgtcc tcggctggat
      241 ggcccacgaa gccgagcatg ccgcggagct ttccgtcgcg gagcgtttcg cggcgtgggc
      301 cgcgcatacg gaagaaggac gcaggcgctt ccgcgatggc gtcctcttca agcctcccca
      361 gcgcgtcgat ccgatgcatc tcgttccggt gcaaacagat gcctcccgcg gttattccgt
      421 gcacacgctg catcacatcc gccgccgcga aggcttcgcc ctcaccgatc cggggacgag
      481 cctcgcgggc gccctcgacg aggcgaacta ctgcatctgg tgccatgagc aggccaagga
      541 ctcgtgctcg aaggggctga aggagaagcc cgcggccgac ggcagcgtgg cattcaagaa
      601 gagccccttc ggcgtgccgc tcgccggctg tcccctcgag gagcgcatct cggaattcca
      661 caagctgaag accgagggct gggccatcgg cgcgctggcc atgatcgtca tcgacaaccc
      721 caccgtgtgc tcgacggggc atcgcatctg caacgactgc atgaagtcgt gcatctacca
      781 gcggcaggat ccggtggaca ttccgcaggc ggagacgcgc acattgaagg atgtgctcga
      841 gctgccgtgg ggcttcgaga tctattcgct gctcacgcgc tggaacccct tgagcctgcg
      901 ccgccccttg cccgccgccg ccaccggcaa gcgcgtgctc gtgatcggca tgggtcccgc
      961 gggcttcacg ctcgcgcatc acctgatgaa cgacggccat gccgtggtcg gcatcgacgg
     1021 gctgaagatc gagcccctcg aggcgcgctt gtccggcgtc gagcccgacg gcacgcgcgt
     1081 cgcgttcgag cccgtccggg atctctccga attgcgcgag cccctcgagc gccgcgtgat
     1141 ggcgggcttc ggcggcgtcg ccgagtacgg catcacggtt cgctgggaca agaacttcct
     1201 caagatggcg cggctcctgg tggagcggcg cgcgcagttc gcgatgttcg gcggcgtgcg
     1261 cttcggcggg acgctcaccg cgcagatggc gttcgagatg ggcttcgacc acatcgcgct
     1321 cgccgcggga gccggcaagc ccacggtcct cgagatgccc cacgggctcg cgcgcggcgt
     1381 gcgcaccgcg tcggatttcc tcatggcgct gcagctgacg ggcgcggcgc gcgaggaatc
     1441 gatcgccaac ctgcagatcc gcctgccgat cgtggtcgtc ggcgggggcc tcacggcgat
     1501 cgacaccgcc accgagtcgc tcgcctacta cccggtgcaa gtggaaaaat tcctcgcgcg
     1561 ctacgagacg ctggtcgtgg agaccagcgt gggctccgtg cgcaacgagt ggaacgagga
     1621 ggagaccgaa gtcgccgagg agtacctcac gcacgcgctg gccatccgcg ccgagcgcgc
     1681 gcgcgcggcg gccgagggcc gcgagcccca tatcctcgag ctcctgcagg cgtggggcgg
     1741 atcgacgatc gcctaccgca agcggctcat cgacagcccg tcgtacacgt tgaaccacga
     1801 ggaggtcgag aaggcgctcg aggaagggat ccgcttcgcc gaaaacctca cgcccctgcg
     1861 cgtcgaggtg gacgaggccg ggcgcgcgtc g
//
LOCUS       DASVII010000348         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_52260, whole
            genome shotgun sequence.
ACCESSION   DASVII010000348 DASVII010000000
VERSION     DASVII010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52260"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="VF309_04580"
     CDS             complement(<1..1915)
                     /locus_tag="VF309_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7465896.1"
                     /translation="MNEAPEIASVLEVLGARRDDLRISQELIEVLPMPVFFKGRDGRY
                     LGVNRAWEEFFGVPRGDIIGGKVVDLYPDSPLVAERHAAMDDELWRHPGSQSYEARLV
                     MRDGRVRHTIYYKATFTRPDGEVAGLIGTIVDITERKEAEQREAIEHAVARYLGASGL
                     SLGDALRGIMQVMCERLDWACAARWSLDEGSNSLHCIETWSVDDAGVKAFLEASARET
                     FVPGTSGMIRRVLSTGNSVWVADVTRKADFIRAGMAAAAGLRGAFALPISVGERVLGA
                     IEFFSREPRNPDKWLLSITVSVGNQIGQLMARRQAEEAMRESEARFRSLTELSSDWYW
                     EQDEQLRFTAMSRGITETIAMPAETYLGKTRWDVGMMGVSAREIEEHKAQLAARLPFQ
                     DFEYGRRDALGEVHYVSASGHPVFDSDGVFRGYRGVGKDITRRKRDEAALRDAHDELE
                     LRARELTRSNEELQQFAYVASHDLQEPLRMVASYTQLLERRYRDKLDGDAKEFMEFIV
                     DGAARMKQLIEDLLAFSRVGTRGRDFREIDSGAALAKALSNLRAAQEASGAVVSHDPM
                     PAVIADASQLTQVFQNLIGNAMKFRGAEPPRIHVGAETRDQVWVFTVRDNGIGLDPQY
                     SDRIFMMFQRLHNK"
     gene            complement(1955..2278)
                     /gene="fdxA"
                     /locus_tag="VF309_04585"
     CDS             complement(1955..2278)
                     /gene="fdxA"
                     /locus_tag="VF309_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX7465897.1"
                     /translation="MAFVVTESCIKCKYMDCVEVCPVDCFHEGPNFLVIDPDECIDCT
                     LCVAECPVDAIFAEDDVPADQKDFIALNAELAKGWKPLVERKPAPPDADQWKEVKAKR
                     DLIEK"
BASE COUNT          404 a          864 c          792 g          444 t
ORIGIN      
        1 ccttgttatg caggcgttgg aacatcatga agatccggtc cgaatattgc ggatcgagcc
       61 cgatgccgtt gtccctcacc gtgaacaccc agacctggtc gcgggtctcg gcgccgacat
      121 ggatgcgcgg aggctcggcg ccgcgaaact tcatggcgtt gccgatgagg ttctggaaga
      181 cctgcgtgag ctgcgaggca tcggcgatca ccgccggcat cgggtcgtgg ctcacgacgg
      241 cgccgctcgc ttcctgcgcc gcgcgaaggt tggagagcgc cttggcgagc gccgcgccgc
      301 tgtcgatctc gcggaaatcg cggccgcgag tgcccacgcg agagaacgcg agaagatcct
      361 cgatcagctg cttcatgcgc gccgcgccgt cgacgatgaa ctccatgaat tccttcgcgt
      421 cgccatcgag cttgtctcgg tagcggcgct ccaggagctg cgtatagctc gcgaccatgc
      481 gcagcggctc ctgcaggtcg tgggaggcga cgtaggcgaa ttgctgcaac tcttcgttgg
      541 agcgcgtgag ctcgcgcgcg cgcagctcga gctcgtcgtg cgcgtcgcga agcgcggcct
      601 cgtcgcgctt tcgccgcgtg atgtccttgc cgactccgcg gtagccgcgg aacacgccgt
      661 ccgagtcgaa tacggggtgc ccgctcgcgc tcacgtagtg cacttcgccg agcgcgtcgc
      721 gccggccgta ctcgaaatcc tggaagggca gccgcgccgc gagctgcgcc ttgtgctcct
      781 cgatctcgcg cgccgagacg cccatcatcc ccacatccca gcgggtcttg cccagataag
      841 tttccgccgg catcgcgatg gtttcggtga tgccgcgcga catcgccgtg aagcgaagct
      901 gctcgtcctg ctcccagtac caatccgagg aaagctcggt gaggctgcgg aagcgcgctt
      961 cgctctcgcg catcgcctcc tcggcctggc gcctcgccat gagctggccg atctggttgc
     1021 cgacgctcac cgtgatcgag agaagccact tgtcgggatt gcgcggctcg cggctgaaga
     1081 actcgatcgc cccgagaacg cgctcgccga cggagatcgg cagcgcgaag gcgccgcgca
     1141 gcccggccgc cgccgccatg ccggcgcgaa tgaagtcggc cttgcgcgtg acgtcggcga
     1201 cccacaccga gttgccggtc gaaaggaccc gccggatcat cccgctggtt ccgggaacga
     1261 atgtctcccg cgcgctcgcc tcgaggaacg ccttcacgcc ggcgtcgtcg acgctccacg
     1321 tctcgatgca gtgcaggctg ttggagccct cgtcgaggga ccagcgcgcg gcgcaggccc
     1381 agtcgaggcg ctcgcacatg acctgcatga ttccgcgcag cgcgtcgccg agcgacaggc
     1441 cgctcgcccc gaggtagcgc gcgaccgcat gctcgatcgc ctcgcgctgt tccgcctcct
     1501 tcctctccgt aatgtcgacg atcgtgccga tcagcccggc gacctcgccg tcggggcgcg
     1561 tgaaggtcgc cttgtagtag atcgtgtggc gcacccggcc gtcgcgcatg acgagccgcg
     1621 cctcgtagct ctgcgacccc ggatgcctcc agagctcgtc gtccatcgcc gcatggcgct
     1681 cggcgaccag gggcgaatcg ggatagaggt cgaccacctt gccgccgatg atgtcgccgc
     1741 gcgggacgcc gaagaattcc tcccacgcgc gattcacgcc gaggtaccgg ccgtcgcgcc
     1801 ccttgaagaa gaccggcatc ggcaggacct cgatcagctc ctgggagatg cgcaggtcgt
     1861 cacggcgcgc tcccaggacc tcgagaaccg atgcgatttc cggagcttcg ttcatggcgc
     1921 cacgctacac cttgggcgcg agccttgcca aaggctactt ctcgatgagg tcccgcttgg
     1981 ccttcacttc cttccactgg tccgcgtcgg ggggcgcggg cttgcgctcg accagcggct
     2041 tccacccctt ggcgagctcg gcattgagcg cgatgaaatc tttctgatcc gcgggcacgt
     2101 cgtcctcggc gaagatcgcg tccacggggc actcggccac gcacagggtg cagtcgatgc
     2161 actcgtcggg atcgatcacc agaaaattcg ggccttcgtg gaaacagtcg accgggcata
     2221 cctcgacgca atccatatac ttgcacttga tgcagctttc ggtgaccacg aatgccatga
     2281 cttgcccttc tattgaccga ttgacgaaaa taccattctg ccacagccgc aaagccgctt
     2341 cccgattgca tcgggacgca cgccgtcagg atttcaggcg cctgttcaat tcgacgaaat
     2401 gatggccctc gcgtcccgcg gcgcggtcct cgaaatactt gcgcaacgtg aattcgccgc
     2461 tcggaaacgc cagctgcgtc cacgggatgt ccgactcgcc gtac
//
LOCUS       DASVII010000349         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_303746,
            whole genome shotgun sequence.
ACCESSION   DASVII010000349 DASVII010000000
VERSION     DASVII010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303746"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF309_04590"
     CDS             complement(<1..369)
                     /locus_tag="VF309_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465898.1"
                     /translation="MAKDYKDAPSPRTDSRLNHPMLLGVIIGMLLGIVISLAVALWLN
                     RLSNPFVEKGKAVEPTPKIGPAQPPPPASAAKAAEDAAKGAKGAEDAKGTKSAPPKAP
                     ERPRFEFYSILPGEKEVTDKE"
     gene            complement(369..>1748)
                     /gene="argS"
                     /locus_tag="VF309_04595"
     CDS             complement(369..>1748)
                     /gene="argS"
                     /locus_tag="VF309_04595"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7465899.1"
                     /translation="VSAGARFAVIAQIHRDAEAFGRGTAGGGRRIMVEFVSANPTGPL
                     HVGHGRQAALGDAVSALLEWQGWRVTREFYYNDAGNQIANLALSVQARVREARGEKAE
                     MPKDGYLGDYIREIAREYVAAHFGDAAGNDLEQIRRFAVSYLRNEQDIDLRAFGVNFD
                     SYFLESSLYTDGKVEETLRMLEKAGHTYESEGALWLRTTDFGDDKDRVMRKSDGTFTY
                     FVPDVAYHLAKYLRGFHSAVNVQGGDHHSTVTRVRAGLQALDAGIPKGYPEYVLHQMV
                     TVMKGGEEVKISKRAGAYVTVRDLIDEVGRDAVRYFFMMRRVDSQLVFDIDVAKSQTD
                     DNPVYYVQYAHARLSSVLREWGGDTATLADADLAPLVSPYEAVLAQKLAEFPEVLARA
                     AAEFSPHLVTYYLHDDVAARLHTYYNAERFLVEDESLKRARLSLVAATRQVLANGLAV
                     LGVSAPVRM"
BASE COUNT          270 a          569 c          597 g          312 t
ORIGIN      
        1 ttccttgtcc gtcacttcct tttcgccggg aaggatcgag tagaactcga agcgcggacg
       61 ctcgggcgcc ttcggcggcg cgctcttcgt gcccttggcg tcttcggccc ccttcgcgcc
      121 cttcgcggca tcctccgcgg ccttggcggc gctcgcgggg ggcggaggct gggccggacc
      181 gatctttggc gtgggctcca cggccttgcc cttctcgacg aacggattcg aaagccggtt
      241 cagccacagc gccaccgcga gcgagatcac gatgcccagc agcatcccga tgatgacgcc
      301 cagcagcatc gggtggttga gccggctgtc ggtgcggggc gagggtgcgt ccttgtagtc
      361 cttggccatc acattctcac gggagcgctc acgccgagga cggcgaggcc gttggcgagc
      421 acttgtcgcg ttgcggcgac gagcgacagg cgcgcgcgct tcaagctttc gtcctcgacc
      481 aggaatcgct cggcattgta gtaggtgtgc aggcgtgccg ccacgtcgtc atgcagataa
      541 tacgtgacga ggtgcggcga gaattccgcc gcggcgcgcg cgagcacttc gggaaactcg
      601 gcgagcttct gcgcgagcac cgcctcgtag ggggagacga gcggcgcgag atcggcgtcg
      661 gcgagcgtgg ccgtatctcc gccccactca cgcagcacgc tcgaaagcct cgcgtgcgcg
      721 tactggacgt agtagacggg attgtcgtcg gtctggctct tcgcgacgtc gatgtcgaag
      781 acgagctgcg aatcgacgcg ccgcatcatg aagaaatagc gcaccgcgtc gcggccgacc
      841 tcgtcgatga ggtcgcgcac cgtgacgtag gcgccggcgc gcttggagat cttcacctct
      901 tcgccgccct tcatcaccgt caccatctgg tgcaggacgt attcggggta gcccttcgga
      961 atgcctgcgt ccagcgcctg gagccccgcg cgcacgcgcg tgacggtgga gtgatgatcg
     1021 ccgccctgaa cattcaccgc ggagtggaag ccgcgaaggt acttggcgag gtggtaggcc
     1081 acatcgggca cgaagtacgt gaacgtgccg tcggacttgc gcatcacgcg gtccttgtcg
     1141 tcgccgaaat cggtggtgcg cagccacagt gcgccctcgg attcgtaggt gtggcccgcc
     1201 ttctcgagca tgcgcagcgt ttcctcgacc ttgccgtcgg tgtagagaga gctctccagg
     1261 aagtaggagt cgaaattgac gccgaaggcg cgcaggtcga tatcctgctc gttgcgaagg
     1321 tagctcaccg cgaagcggcg aatctgctcg aggtcgttgc ccgcggcgtc gccgaagtgc
     1381 gccgcgacgt attcgcgcgc gatctcgcgg atgtagtcgc cgagataccc gtccttgggc
     1441 atctcggctt tttccccacg ggcttcgcga acgcgcgcct gcacggagag cgcgagattg
     1501 gcgatctggt tcccggcgtc gttgtagtag aactcgcgcg tgacgcgcca gccctgccac
     1561 tcgaggagcg ccgacaccgc gtcgccgagc gcggcctggc gcccgtgacc cacgtggagc
     1621 ggacccgtcg gattggcgga gacgaattcc accatgatcc tgcggccgcc gccggccgtg
     1681 ccgcggccga atgcctctgc atcgcggtgg atctgcgcga tcaccgcgaa gcgcgcgccg
     1741 gcggatac
//
LOCUS       DASVII010000350         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_411908,
            whole genome shotgun sequence.
ACCESSION   DASVII010000350 DASVII010000000
VERSION     DASVII010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411908"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..736
                     /gene="rnc"
                     /locus_tag="VF309_04600"
     CDS             14..736
                     /gene="rnc"
                     /locus_tag="VF309_04600"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7465900.1"
                     /translation="MTRKAARGGEVSDLAELERRLGHQFRDPDLARQALTHRSFGAPH
                     NERLEFLGDSLLNCAVATILYERFPQLPEGDLSRLRAALVNQRSLSEVAVRLGLGDRL
                     RLGEGELKSGGFRRPSILADSLEALLGAVFLDAGFEATRAVVATLLTDKLDSAADKPV
                     DKDPKTALQEHLQGRGLALPRYAVQKTEGEAHEQTFIVECRVDDLGIVANGQGTSRRA
                     AEQAAAEGVLKELEKAPRRKKT"
     gene            733..1683
                     /gene="era"
                     /locus_tag="VF309_04605"
     CDS             733..1683
                     /gene="era"
                     /locus_tag="VF309_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7465901.1"
                     /translation="MKEKDQARAARRDEKARGAAKDKAPFRTGTVAIVGRPNVGKSTL
                     LNHLVGAHVAITSRKAQTTRHRIQGILTDPVSQFIFVDTPGYQTQHGNALNRWMNRTV
                     GQVATEVDVVVFVIDAGHWDPRDEPLLGLIPEKVPVILALNKVDKVEDKAQIAETLVE
                     ANAKRDFAALVPISASKGTQLKALLGEIRSRLPQQEAIYEADEITDKSERFLAAELVR
                     ERIFRLLGDELPYGIAVDIEKFETEGNLRRISATIYVDKPAHKGMVIGEKGATLKRIG
                     TEARHAMQELFGSRVHLELWVRVKSGWADSEISLRQLGYK"
     gene            1688..>2014
                     /gene="recO"
                     /locus_tag="VF309_04610"
     CDS             1688..>2014
                     /gene="recO"
                     /locus_tag="VF309_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7465902.1"
                     /translation="MDKGYVLHTYPYRETSLILQVWTEKHGRMGLVAKGARRPKSASR
                     SVLIPFQPLSLDWFGRGELRTLKTAEPASPATPLGGSSLLSAFYMNELLLKLTTRDDP
                     HEGLFAA"
BASE COUNT          366 a          663 c          702 g          283 t
ORIGIN      
        1 tgactacctc gaagtgacac gcaaggcggc gcgcggcggc gaggtctcgg acctcgccga
       61 gctggaacgg cgcctgggac accagtttcg cgatcccgat ctcgcgcgcc aggcgctcac
      121 ccatcgcagc ttcggcgcgc cgcacaacga gcggctcgag ttcctcggcg attcgcttct
      181 caactgcgcg gtcgcgacga tcctctacga gcgtttcccg cagctgcccg agggagacct
      241 gtcgcgattg cgcgcggcgc tcgtgaacca gcgatcgctt tcggaggtgg cggtgcggtt
      301 gggtctggga gaccggctga ggctcggcga gggagagctc aagagcggag ggtttcgccg
      361 gccgtcgatc ctcgccgatt cgctcgaggc gttgctcggc gcggtcttcc tcgacgccgg
      421 gttcgaagcc acgcgcgcgg tggtcgcgac gctcctcacg gacaagctcg acagcgccgc
      481 agacaagccc gtggacaagg atccgaaaac ggcgctgcag gagcacctgc aggggcgcgg
      541 gctcgcgctg ccgcgctacg cggtgcagaa gaccgagggc gaggcgcacg agcagacctt
      601 catcgtcgaa tgcagggtgg acgacctggg catcgtggcg aacgggcagg gaacgagccg
      661 gcgcgcggcc gagcaggccg cggccgaagg cgtgctgaag gaactggaga aggcgccacg
      721 gcgcaagaag acatgaaaga aaaagaccag gcgcgagccg cgagaaggga cgagaaggcg
      781 cgcggcgccg cgaaggacaa ggcccccttc aggaccggca cggtcgcgat cgtggggcgg
      841 ccgaacgtgg ggaagtcgac gctcctcaac cacctcgtcg gcgcgcacgt ggcgatcacc
      901 tcgcgcaagg cgcagaccac gcgccaccgc atccagggca tcctcacgga tccggtctcg
      961 cagttcatct tcgtcgacac gccgggctac cagacgcagc acgggaacgc gctcaaccgc
     1021 tggatgaatc gcaccgtcgg ccaggtcgcg accgaggtcg acgtggtggt gttcgtgatc
     1081 gacgccggcc attgggatcc gcgcgacgag ccgttgctcg ggctcattcc cgagaaggtc
     1141 ccggtgatcc tcgcgttgaa caaggtcgac aaggtcgagg acaaggcgca gatcgcggag
     1201 accctcgtcg aggcgaacgc gaagcgcgac ttcgccgcgc tcgtgccgat cagcgcttcg
     1261 aagggcacgc agctgaaggc gctcctcggc gagatccgct cgcggcttcc gcaacaggaa
     1321 gcgatctacg aggccgacga gatcaccgac aagtcggagc gcttcctcgc cgcggagctg
     1381 gtgcgcgagc ggatctttcg cctgctcggc gacgagctgc cctacggaat cgccgtggat
     1441 atcgaaaagt tcgagaccga gggcaacctg cgccgcatct cggcgaccat ctacgtcgac
     1501 aagcccgccc acaagggcat ggtgatcggc gagaagggcg cgaccctgaa gcgcatcggc
     1561 accgaggcgc gccacgcgat gcaggagctc ttcggctcgc gcgtgcacct cgagctgtgg
     1621 gtgcgcgtga agagcggctg ggccgacagc gagatctcgc tgcggcagct cgggtacaaa
     1681 tgaaggcatg gacaagggtt acgttcttca cacctaccct taccgcgaga ccagcctgat
     1741 cctgcaggtg tggacggaga agcacgggcg catgggcctg gtcgccaagg gcgcgcgccg
     1801 tccgaagtcg gcgagccgca gcgtgctgat ccctttccag ccgctctcgc tcgactggtt
     1861 cggccgcggc gagctgcgca cgctgaagac ggcggaaccg gcttcacccg cgacgcccct
     1921 cgggggctcc agccttcttt ccgccttcta tatgaacgag ctcctcctca agctcacgac
     1981 gcgcgacgat ccgcacgagg ggctcttcgc ggcg
//
LOCUS       DASVII010000351         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_555833,
            whole genome shotgun sequence.
ACCESSION   DASVII010000351 DASVII010000000
VERSION     DASVII010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555833"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VF309_04615"
     CDS             <1..428
                     /locus_tag="VF309_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7465903.1"
                     /translation="IYATDINQVALATAEAGVYALDRIAEFSASYLQAGGTGSLADYY
                     TAAYNSAIFDRRLKAQIVFTEHSLATDTVFSEVELISCRNVLIYFDRALQTRAYGLFH
                     DSLAPRGFLGIGSKESLRFAKHSAAFEEFAPAERIYRKR"
     gene            425..1042
                     /locus_tag="VF309_04620"
     CDS             425..1042
                     /locus_tag="VF309_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX7465904.1"
                     /translation="MSSPRKARNKAILEIEAIAIGASAGGVDALAILFEALPAPFKPA
                     IVVVVHIPANRPSMLAELYRARCRLPVREALDKEPIEPGTIYFAPPDYHLLVEKDKSL
                     SLSHDPPVAFSRPSIDVLFESAALAYGPALLGIVLSGANSDGANGLVAVRQAGGHAWV
                     QEPGDALANAMPAAAIANAGADLVVPIRDMAERLVQLRSGRAVAI"
     gene            1054..>1525
                     /locus_tag="VF309_04625"
     CDS             1054..>1525
                     /locus_tag="VF309_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465905.1"
                     /translation="MDSDINVLVVDDVPENLVAISALLTRPGVRVLEAPSATAALEIL
                     LVEEVAMALVDVQMPLMDGFELAELIRGSERTRNTPLIFLTASVSDPGRTFRGYEAGA
                     VDFLHKPIDPSVLRGKVEVFVDLHAQKRKLARQLEELRAALRMNEVFVAVLGHDL"
BASE COUNT          225 a          565 c          488 g          247 t
ORIGIN      
        1 tcatctacgc gaccgacata aaccaggtgg cgctcgccac cgccgaggcc ggggtgtacg
       61 ccctcgatcg catcgcggag ttcagcgcga gctacctgca ggcgggcggc acgggctcgc
      121 tcgccgacta ctacaccgcc gcgtacaaca gcgcgatctt cgaccggcgc ctgaaggcgc
      181 agatcgtctt caccgagcac agcctcgcta ccgacaccgt cttttccgaa gtcgagctca
      241 tctcctgccg caacgtcctc atctacttcg accgcgcgct gcagacgcgc gcgtatggcc
      301 tcttccacga ttccctcgcg ccgcgcggct tcctcggcat cggctccaag gagagcctgc
      361 gcttcgcgaa gcactccgcc gcgttcgagg agttcgcgcc ggccgagcgc atctaccgca
      421 agcgatgagc tccccccgca aggcgcgcaa caaggccatc ctcgagatcg aggcgatcgc
      481 gatcggcgct tccgcgggcg gcgtggacgc gctcgccatc ctcttcgagg cgctgcccgc
      541 gcccttcaag cccgcgatcg tggtcgtggt ccacatcccc gcgaaccggc ccagcatgct
      601 cgccgagctc tatcgcgcgc gctgccgcct gccggtgcgc gaagcgctcg acaaggagcc
      661 tatcgagccc ggcaccatct atttcgcgcc gccggactac catctgctgg tggagaagga
      721 caaatcgctc tccctgtccc acgatccccc ggtggcattt tcccggccga gcatcgacgt
      781 gctgttcgag tccgcggcgc tcgcgtacgg gcccgcgctc ctcggcatcg tgctttccgg
      841 cgccaattcc gacggcgcca acggccttgt cgcggtgcgc caggcgggcg gccatgcctg
      901 ggtccaggag cccggtgacg cgctcgccaa tgccatgccc gcggccgcca tcgccaacgc
      961 cggggccgac ctcgtggtgc cgatacgcga catggccgag cgcctcgtgc agctgcgctc
     1021 ggggcgcgcg gtcgcgatct aatagaatgg gccatggact ccgacatcaa cgttctggtc
     1081 gtggacgacg ttcccgagaa cctcgtcgcg atctcggcgc tgctcacgcg ccccggagtg
     1141 cgcgtgctcg aggcgccctc ggccacggcc gcgctcgaga tcctcctcgt ggaggaggtg
     1201 gcgatggcgc tggtggacgt gcagatgccg ctgatggacg gcttcgagct ggcggagctc
     1261 atccgcggga gcgagcgcac gcgcaacacc ccgctcatct tcctcaccgc ttcggtgtcc
     1321 gaccccggcc gcacgtttcg cggctacgag gccggcgccg tggacttcct gcacaagccg
     1381 atcgatccca gcgtgctgcg cggcaaggtg gaggtgttcg tcgatctcca cgcgcagaag
     1441 cgcaagctcg cgcgccagct cgaggagctg cgcgcggcgc tgcgcatgaa cgaggtgttc
     1501 gtcgcggtgc tgggacacga cctgc
//
LOCUS       DASVII010000352         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_447680,
            whole genome shotgun sequence.
ACCESSION   DASVII010000352 DASVII010000000
VERSION     DASVII010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447680"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1592
                     /gene="lon"
                     /locus_tag="VF309_04630"
     CDS             <1..>1592
                     /gene="lon"
                     /locus_tag="VF309_04630"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7465906.1"
                     /translation="ILLQNSPEVDEPGPDEMHWVGTTANVLRYVTTDGANHAILKGEQ
                     RFRVLQFLEGYPFTVARVQLIEEAKVDDPEVEGRARTLRARAREILDLLPQVPAEIAT
                     AFQGVETPSLLADFIAGMMDVSAQDKQDLLETFDLRTRLDRILGMLAHRIEVLKVSRD
                     IEEKTRESLGDANRKHVLREQKRAIEKELGEGEEGSDADLAELAKAIADAKMPEEVDK
                     QARKELKRLERMPEAAAEYSMVRSYLEWLVELPWASEPEPAIDIAEARRILDEDHYGL
                     EKIKRRILEHLAVRKLNPNGKSPILCFVGPPGVGKTSLGQSIARATGRKFARVSLGGV
                     HDEAEIRGHRRTYVGALPGNIVQSLRKVETRNAVMMLDEIDKLGAGGFHGDPASAMLE
                     VLDPEQNSTFRDNYLAVPFDLSRVMFIATANVLDTIPGPLRDRMEVIQLPGYTAQEKL
                     QIARRYLVARQLKATGVSAEQVTLDDEALLAIIHDYTREAGVRNLEREIGNVIRHVAV
                     RIAEGKEQSMKIGAADLAAILGA"
BASE COUNT          292 a          537 c          540 g          223 t
ORIGIN      
        1 attctcctgc agaattcccc ggaggtcgac gagcctggcc ccgacgagat gcattgggtg
       61 ggcaccaccg cgaacgtgct tcgctacgtc acgaccgacg gcgcgaatca cgcgatcctg
      121 aagggggagc agcgcttccg cgtgctgcag ttcctcgaag gctatccgtt caccgtcgcc
      181 cgcgtgcagc tcatcgagga ggcgaaggtc gacgatcccg aggtggaggg tcgcgcccgg
      241 acgctgcgcg cgcgtgcgcg cgagattctc gatctcctcc cccaggtgcc cgcggagatc
      301 gcgaccgcgt tccagggcgt cgagaccccg tcgctcctcg ccgatttcat cgcgggcatg
      361 atggacgtga gcgcgcagga caagcaggac ctcctcgaga cgttcgacct caggacccgc
      421 ctcgaccgga tcctcgggat gctcgcgcat cgcatcgagg tgctcaaggt ctcgcgcgac
      481 atcgaggaga agacgcgcga gtcgctcggc gacgccaacc gcaagcacgt cctgcgggag
      541 cagaagcgcg ccatcgagaa ggagctcggc gaaggcgagg agggctccga tgcggatctc
      601 gcggagctcg cgaaggcgat cgccgacgcg aagatgcccg aggaggtcga caagcaggcg
      661 cgcaaggagc tgaagcgcct cgagcgcatg cccgaagccg cggccgagta ttcgatggtc
      721 cgctcgtacc tcgaatggct ggtcgagctg ccgtgggcct cggagcccga gcccgcgatc
      781 gacatcgcgg aggcgcggcg catcctcgac gaggaccact acggcctcga gaagatcaag
      841 cggcgcatcc tcgagcatct cgcggtgcgc aagttgaatc ccaacgggaa gagcccgatc
      901 ctctgcttcg tcggcccgcc cggcgtgggc aagacctcgc tcggccagag catcgccagg
      961 gcgacggggc gcaagttcgc gcgcgtgagc ctgggcggcg tgcacgatga agcggaaatc
     1021 cgcggccacc gccgcaccta cgtgggcgcc cttccgggca acatcgtcca gagcctgcga
     1081 aaggtggaaa cgcgcaacgc cgtgatgatg ctcgacgaga tcgacaagct cggcgcgggc
     1141 ggcttccacg gcgatcccgc ctcggcgatg ctcgaggtgc tcgatcccga gcagaactcg
     1201 accttccgcg acaactacct cgcggtgccg ttcgatctct cgcgcgtgat gttcatcgcc
     1261 acggcgaacg tgctcgacac catccccggc ccccttcggg accggatgga agtgatccag
     1321 ctcccggggt acaccgcgca ggagaaattg cagatcgcgc gccgttacct ggtggcgcgc
     1381 cagctcaagg cgaccggcgt aagcgccgag caagtcacgc tcgacgacga ggcgttgctc
     1441 gcgatcatcc acgactacac gcgcgaagcg ggcgtgcgca acctcgagcg cgagatcggg
     1501 aacgtgatcc gccacgtggc ggtacgcatc gccgagggca aggagcagtc gatgaagatc
     1561 ggcgccgcgg atctcgcggc catcctcggc gc
//
LOCUS       DASVII010000353         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_159885,
            whole genome shotgun sequence.
ACCESSION   DASVII010000353 DASVII010000000
VERSION     DASVII010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159885"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VF309_04635"
     CDS             complement(<1..579)
                     /locus_tag="VF309_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7465907.1"
                     /translation="MFENFETLDIETSGARIHLRKAGAGPPLLLLHGNPLTHVMWHKI
                     APRLAEEFTVVATDLRGYGDSSKPPGGEQSIGYSFRSMAVDQVEVMEKLGFDRFFVAG
                     HDRGGRVAHRMALDSPGRVEKVAFLDIVPTHHMLSNIRKTWAVDSYHWFFMAQPYDYP
                     EKMIEAYGFERYIRKKLDKPGVGLSAFTPEAMA"
     gene            complement(755..2182)
                     /locus_tag="VF309_04640"
     CDS             complement(755..2182)
                     /locus_tag="VF309_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX7465908.1"
                     /translation="MSATETSPPQAQTPALTDYVAGFVVGARAKDIPPDVARLGKRSV
                     LDGIGLALAGAASQQGAISRRYLEGLGIGSSHGSTVIGAKLRLPARFAAFANGLTIHA
                     DDYDDTQLAVAKDRVYGLLTHPTAPALPPVLALAERDARSGADMMLAYQVGVEVECKV
                     AEAIFPRHYQHGFHATATCGSIGAAAGAAKILGLNREATRRALSIGATQAGGLRENFG
                     TMTKPFHAGRAAENGVVAAELAALGFTASPNGLEADRGFFRAAGGGYSPEMIQNKLGD
                     PWTFHFPGVSIKPHPSGSLTHPGMAVMMEMIMLHGIRPRDVKRVAVGTNHNMPNALIH
                     HKPANELQAKFSMEFCMAILILERKAGLEQFTDEVVNRPDVQAMIAKVNFGVHPDAEA
                     AGFDKMTTIIEIEMNDGQVFKGQADFGKGSPANPMSDDELSEKFRQCAAWGGLDGATT
                     KKVLELVWKIEELKDVNELTKLLRR"
BASE COUNT          342 a          743 c          761 g          415 t
ORIGIN      
        1 ggccatcgcc tcgggcgtga aggcggaaag tcccacgccc ggcttgtcga gcttcttgcg
       61 gatgtagcgc tcgaagccgt aggcctcgat catcttctcg gggtagtcgt agggctgcgc
      121 catgaagaac cagtggtacg aatcgaccgc ccaggtcttc ctgatgttgc tcagcatgtg
      181 gtgcgtcggc acgatgtcca ggaacgcgac cttctcgacg cgcccgggcg aatcgagcgc
      241 catgcgatgg gcgacgcgcc cgccgcgatc gtggccggcg acgaagaagc ggtcgaagcc
      301 cagcttctcc atcacctcga cctggtccac cgccatggag cggaaggaat agccgatgct
      361 ctgctcgccg cccgggggct tggagctgtc gccgtagccg cgcagatcgg tcgcgacgac
      421 cgtgaattcc tcggcgagcc gcggcgcgat cttgtgccac atcacgtgcg tcaacggatt
      481 gccgtgcagc agcagcagcg gcggccccgc gcctgccttc cgcagatgaa tgcgtgcgcc
      541 ggaagtctcg atgtcgaggg tttcgaagtt ttcgaacatc aaagctttgg atccgcagat
      601 aaacgcagat gaacgcggta aaacccttcc tgcttccaat ggttcatgcg catcattcca
      661 tcgatcgtgc agcccatacc agcattgcct ttggggttac cgcgttcatc tgcgtttatc
      721 tgcggatcca aagcctttcg ctcgcatcac tccctcaccg ccgcaagagc ttcgtgagct
      781 cgttcacgtc cttgagctcc tcgattttcc agacgagctc gagcaccttc ttcgtggttg
      841 cgccgtcgag cccgccccac gcggcgcact ggcggaactt ctccgagagc tcgtcgtcgc
      901 tcatggggtt ggcggggctg cccttgccga agtccgcctg gcccttgaac acctggccgt
      961 cgttcatctc gatctcgatg atggtggtca tcttgtcgaa gcccgcggcc tcggcgtcgg
     1021 gatgcacgcc gaaattcacc ttcgcgatca tcgcctgcac gtcggggcgg ttcaccacct
     1081 cgtccgtgaa ctgctcgagc ccggccttgc gctcgaggat gaggatcgcc atgcagaact
     1141 ccatgctgaa cttcgcctgc agctcgttcg ccggcttgtg atggatgagc gcgttgggca
     1201 tgttgtggtt cgttcccacc gccacgcgct tcacgtcgcg gggacggatg ccgtggagca
     1261 tgatcatctc catcatcacc gccatgcccg ggtgcgtgag cgagcccgag ggatgcggct
     1321 tgatggagac gccggggaaa tgaaaggtcc acggatcgcc cagcttgttc tggatcatct
     1381 cggggctgta gccgccgccc gccgcgcgga agaagccgcg gtcggcctcg aggccgttgg
     1441 gcgaggccgt gaatcccagc gcggcgagct ccgcggcgac gacgccgttc tccgcagcgc
     1501 gtcccgcgtg gaagggcttg gtcatggtgc cgaagttctc gcgcagaccg cccgcctggg
     1561 tcgctccgat ggaaagcgcg cgccgcgtcg cctcgcggtt cagcccgaga atcttcgccg
     1621 cgcccgccgc cgcgccgatg gagccgcacg tcgcggtggc gtggaatccg tgctggtagt
     1681 ggcgcgggaa gatcgcctcg gcgaccttgc attcgacctc cacgccgacc tggtaggcga
     1741 gcatcatgtc cgcgccgctg cgcgcgtcgc gctccgcgag cgccagcacc ggcggcagcg
     1801 cgggcgccgt cgggtgcgtg agcagtccgt acacgcgatc cttggcgacg gcgagctgcg
     1861 tgtcgtcgta gtcgtcggcg tggatcgtga ggccgttggc gaacgccgcg aatcgcgccg
     1921 gcagccgcag cttggcgccg atcacggtgc tgccatggga cgatccgatg ccgaggccct
     1981 cgaggtagcg ccgcgaaatc gcgccctgct gcgacgcggc gccggcgagc gcgagcccga
     2041 tgccgtcgag caccgagcgc ttgcccaggc gcgccacgtc cggcggaatg tccttcgcgc
     2101 gcgcgccgac gacgaagccg gcgacgtagt cggtgagggc aggagtttgc gcttgcggcg
     2161 gcgaggtttc agtggcgctc atgttccctt tcggtctttg atgggttccc gcgttggcgg
     2221 gaaaggcggg aggatgcgag gcgcgcggcg aggcgaacgg c
//
LOCUS       DASVII010000354         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_447763,
            whole genome shotgun sequence.
ACCESSION   DASVII010000354 DASVII010000000
VERSION     DASVII010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447763"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF309_04645"
     CDS             <1..703
                     /locus_tag="VF309_04645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7465909.1"
                     /translation="YAPANVDAVRKLVRERYFDGSFVIRSQDNYVVQWARPEDDPRAK
                     AMAVVRLKPEFARPMDAKLPFTRLAYADTYAPEVGFSGGFPVARDPKRKETWLVHCYG
                     SVGVGRDNAADSGNGSELYAVIGQSPRNLDRNITLVGRVVQGIELFSVMPRGKAVMGF
                     YEKPSEWIAIRAIRVAADVPAAERTNLEALRTDSATFKAWVASRASRREEWFKEPVGR
                     VGVCNVPLPVRAASR"
     gene            complement(700..1365)
                     /locus_tag="VF309_04650"
     CDS             complement(700..1365)
                     /locus_tag="VF309_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX7465910.1"
                     /translation="MSNPLPALSLLETRVLGVLVEKQHTVADSYPLTLNALVSGANQK
                     TSRDPVIEATDAEVQAAVDHLKSLSLVMESSGGRVMRYAQNVGKVIGTPPQSVALLAV
                     LFLRGPQTAGELRINSDRLHKFGDILAVEGFLHELADRESGALVRELPKQPGARETRW
                     VHLMSGEPSIPEAAAPAASGADAVSVSELAAMKANIDQLQREVAELRETVARLGKELG
                     ISA"
     gene            complement(1378..1515)
                     /locus_tag="VF309_04655"
     CDS             complement(1378..1515)
                     /locus_tag="VF309_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX7465911.1"
                     /translation="MFVQIGPLLALIAGILILVMPKLLNYIVAIYLIAFGLLGLLGGR
                     I"
     gene            complement(1584..2282)
                     /locus_tag="VF309_04660"
     CDS             complement(1584..2282)
                     /locus_tag="VF309_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7465912.1"
                     /translation="MAATPVFFLPGLLEDADAFEHQIERLGKLAPCSVADLTLADSIA
                     GLAKDALRQAPPGRFCLCGHSMGGYVALEVMRQAPDRVERLALLNTHARPDTPEATEN
                     RVRLMALAEKDFPEVIRTLMPKLVAPEHLADSDTEAMITEMALSIGKEAFARQERAII
                     GRIDSRPHLAAIRCRTLVVAARLDALMPVAILEELAHGIPGAVLAIVEHSGHMASIEQ
                     PEEVAGLLASWLEG"
BASE COUNT          361 a          735 c          846 g          365 t
ORIGIN      
        1 gtacgcgccc gcgaacgtcg acgcggtgag aaagctcgtg cgcgaaagat atttcgacgg
       61 cagcttcgtg atccgctcgc aggacaacta cgtcgtgcag tgggcgcggc ccgaggacga
      121 cccgcgcgcg aaggcgatgg cggtcgtgag attgaagccc gagttcgcgc ggccgatgga
      181 cgcgaagctg ccgttcacgc gcctcgcgta tgcggatacc tacgcgccgg aggtcggatt
      241 ctcgggcgga tttcccgtcg cgcgcgatcc gaagcgcaag gaaacgtggc tcgtgcactg
      301 ctacggctcg gtgggcgtgg gccgcgacaa cgcggccgat tccggcaacg gctccgagct
      361 gtacgcggtc atcgggcaat cgccgcgcaa cctcgatcga aacatcaccc tcgtgggccg
      421 cgtcgtgcag ggcatcgagc tcttctccgt gatgccgcgc gggaaggccg tgatgggctt
      481 ctacgagaag ccgtcggagt ggatcgcgat ccgcgcgatt cgcgtggcgg ccgacgtgcc
      541 cgccgcggag cgcaccaatc tcgaggcgtt gcgaaccgac agcgcgacct tcaaggcgtg
      601 ggtggcctcg cgcgcgagcc ggcgcgagga atggttcaag gagccggtgg gacgcgtggg
      661 cgtgtgcaac gtgccgctgc ccgttcgggc ggcgtcccgc taggcggaga ttcccagctc
      721 cttgccgagg cgcgccacgg tctcgcgcag ctcggccact tcccgctgca gctggtcgat
      781 gttggccttc atcgccgcga gctcgctcac cgagaccgcg tcggcgcccg aggcggcggg
      841 cgcggcggct tcgggaattg aaggctctcc ggacatcagg tgaacccatc gcgtctcgcg
      901 tgcgcccggc tgcttgggca gctcgcgcac cagcgcgccg ctctcgcgat cggccaattc
      961 gtgcaggaat ccctcgaccg cgaggatgtc gccgaacttg tgcagccggt cgctgttgat
     1021 gcgaagctcc cccgccgtct gcggcccgcg caggaagagc accgcgagga gggccaccga
     1081 ttgcggcggc gtgccgatca ccttgcccac gttctgcgcg tagcgcatca cgcgcccgcc
     1141 gctcgactcc atcacgagcg aaagcgactt caggtgatcg accgcggcct gcacctcggc
     1201 atcggtcgcc tcgatcacgg gatcgcggct ggtcttctgg ttggcgccgg agacgagcgc
     1261 gttcagcgtg agcgggtagg aatccgcgac ggtgtgctgc ttctcgacga gcacgccgag
     1321 gacgcgggtc tcgagaaggg aaagcgcggg aaggggattc gacatcctct cagtttgtca
     1381 gattcgcccg ccgaggaggc cgaggagccc gaatgcgatg aggtagatgg cgacgatgta
     1441 gttgaggagc ttcggcatga cgaggatcag gatgccggcg ataagtgcga gaaggggacc
     1501 gatttgcacg aacatgaatg ttctccttgt ggcggtggcc tccctcagac tcgctccatc
     1561 tccgggagtt caccgccgcg ggatcagcct tcgagccacg aggcgaggag gcccgcgacc
     1621 tcttcgggtt gctcgatcga cgccatgtgg ccggagtgct cgacgatcgc gagcaccgcg
     1681 ccgggaatgc cgtgggcgag ctcctcgagg atcgcgacgg gcatcagcgc gtcgaggcgc
     1741 gcggccacga ccagcgtcct gcaacggatc gcggcgaggt gcgggcggct gtcgatgcgc
     1801 ccgatgatcg cgcgctcctg gcgcgcgaac gcttccttgc cgatgctgag cgccatctcc
     1861 gtgatcatgg cctcggtgtc ggagtcggcc aggtgctccg gggcgacgag cttcggcatc
     1921 aacgtgcgga tgacctcggg aaaatccttc tcggcgagcg ccatcagccg cacgcgattc
     1981 tcggtggcct cgggcgtgtc ggggcgcgcg tgcgtgttga ggagcgcgag ccgctcgacg
     2041 cggtcgggtg cctggcgcat cacctcgagg gcgacgtagc ctcccatcga atggccgcag
     2101 aggcagaagc ggcccggggg ggcttgccgc agcgcgtcct tcgcgaggcc ggcgatggaa
     2161 tcggcgagcg tcaggtcggc gacgctgcag ggcgcgagct tgccgaggcg ctcgatctgg
     2221 tgctcgaacg cgtcggcgtc ctcgaggagg cctggaagga agaagaccgg ggtcgcagcc
     2281 attgggagtc ggcagccggg gcgagga
//
LOCUS       DASVII010000355         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_483765,
            whole genome shotgun sequence.
ACCESSION   DASVII010000355 DASVII010000000
VERSION     DASVII010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483765"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VF309_04665"
     CDS             <1..771
                     /locus_tag="VF309_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465913.1"
                     /translation="DLGISAEGPIRAVPDLPPRAMSFVALMAKVPAMVFATIVVTFLV
                     AFPVAIAWLAWTVRREPSLAAVPVVLVMLIAIAGYSLATTAFGDGIFEAERHNWLGSL
                     ATLAAVGMLPIVVWQLTRDLVSARIAVAAAFGVMLLATGWVLWTRQGNLAIGALDRVV
                     AEQARSLQLTGWALDPWGVRRVYATVGGGPQTEGTRGIERRDLEAAYPGYPESVSAGF
                     RITLAPNAWRENEQMRVYVENSAGAITEIDRRRIRLRP"
     gene            complement(731..1174)
                     /locus_tag="VF309_04670"
     CDS             complement(731..1174)
                     /locus_tag="VF309_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465914.1"
                     /translation="MARALIVHRAESFASRHGREESESRLKSAMAGIRPRRLRFESRW
                     SEAPSGLSLHAEFHPAARTQRMLQASSLMLVALIAASAWALASDQVERTLAFVLALAT
                     VLWVLALPLVVAAFGAQREAEEAGIRRALKRALTDEDESGADRSR"
     gene            complement(1204..>1679)
                     /locus_tag="VF309_04675"
     CDS             complement(1204..>1679)
                     /locus_tag="VF309_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465915.1"
                     /translation="ISPPESPGSNAATPLAKEGRALAEGPAPFPAATVPATVNAPAVT
                     LVVPAASAYAPAPSPPASAAGALATPAEASAQSAVQASAARAAPRLKAEAADAIQPVQ
                     RAAISQGGIDRELERIAKLREEGRDADADKALEQFNRDHPGYRIADAMWQRVKPR"
BASE COUNT          214 a          585 c          619 g          261 t
ORIGIN      
        1 gacctgggca tctccgccga agggccgatt cgcgccgtgc ccgacctgcc gcctcgagcg
       61 atgtctttcg tcgcgctcat ggcgaaggtt ccggcgatgg tcttcgcgac gatcgtcgtg
      121 acgttcctcg tcgcgtttcc ggtggcgatc gcatggctcg cgtggaccgt gcgtcgcgag
      181 ccgagcctgg cggccgtgcc cgtggtgctc gtgatgctga tcgcgatcgc gggctattcg
      241 ctggcgacca cggccttcgg cgacggcatt ttcgaagccg agcggcacaa ctggctggga
      301 tcgctcgcga cgcttgccgc cgtgggcatg ctgccgatcg tcgtctggca actcacgcgc
      361 gatctcgtga gcgcgcgcat cgccgtcgcg gcggcattcg gcgtgatgct gctcgcgacc
      421 ggctgggtcc tctggacgcg ccagggaaac ctggcgatcg gcgcgctcga ccgggtagtc
      481 gcggagcagg cgcgatcgct ccagctgacc ggctgggccc tcgatccctg gggcgtgcgc
      541 cgggtctacg cgaccgtggg cggcggcccg cagaccgagg gcacgcgcgg catcgagcgc
      601 cgggatctcg aggccgcata tcccggatat cccgagtcgg tctccgcggg cttccggatc
      661 acgcttgccc cgaacgcgtg gcgcgagaac gagcagatgc gcgtctacgt ggagaacagc
      721 gccggcgcga tcaccgagat cgatcggcgc cggattcgtc ttcgtccgta agggcgcgct
      781 tcagcgccct gcggatgccg gcctcctcgg cctcgcgctg cgcgccgaag gccgcgacca
      841 cgagaggcaa cgcgagaacc cacagcaccg tcgcgagcgc gaggacgaac gcgagcgtgc
      901 gttccacttg atcggaggcg agcgcccacg cgctcgccgc gatgagcgcg acgagcatca
      961 gcgaggaggc ctgcagcatc cgctgcgtgc gcgccgcggg gtggaactcg gcgtgcagcg
     1021 acaatccgga gggcgcctcg ctccagcggc tttcgaagcg caggcgcctg ggcctgatcc
     1081 ccgccatcgc ggacttcagc cgcgattcgg attcctcgcg cccgtggcgg ctcgcgaagc
     1141 tttcggcgcg gtgcacgatg agcgcgcggg ccatccgccg catctcggag gccatcggcg
     1201 ctcctagcgg ggcttcaccc gctgccacat cgcgtcggcg atgcgatagc cgggatggtc
     1261 gcggttgaat tgctcgagcg ccttgtcggc gtcggcatcg cggccttcct cgcgcagctt
     1321 cgcgatccgc tcgagctcgc ggtcgatgcc gccttgcgaa atcgccgcgc gctggacggg
     1381 ctgtatggcg tcggcggcct cggccttgag gcgtggtgcc gctcgcgccg cggacgcctg
     1441 caccgcggat tgggccgacg cttccgccgg ggtcgcgagc gctccggcgg ccgaggccgg
     1501 gggcgatggc gcgggcgcgt acgcggacgc cgcgggcacc accagggtca ccgccggcgc
     1561 gttcaccgtc gcggggacgg tcgcagcggg aaatggcgcg ggcccttcag ccagcgcacg
     1621 tccttctttc gcaagcggcg tcgcggcgtt cgagccggga gactcgggcg gagagattc
//
LOCUS       DASVII010000356         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_699589,
            whole genome shotgun sequence.
ACCESSION   DASVII010000356 DASVII010000000
VERSION     DASVII010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699589"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /gene="lptD"
                     /locus_tag="VF309_04680"
     CDS             <1..1656
                     /gene="lptD"
                     /locus_tag="VF309_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX7465916.1"
                     /translation="FFLGMPILYLPYMTFPLDNKRRSGFLAPTFGTSGQSGFEASLPY
                     YWNIAPNMDATITPKIFTKRGIQVGTEFRYLYPKYSGQFDTEYLPNDKIREIDRYFLG
                     WRHAQQLWWPGWSFAVNAQKVSDDNYFRDLTTKIALTSQTNLPRDAILAYNNDVWSVS
                     ARVLGYQTLEDPLNQVPIPYNILPQILAFGSKQNVNGFDWQLTSELSNFRHPTLPNGQ
                     RFIAYPSVSLPMRQSYGYVTPKIGFNFTRYNMDQNTSGLESSSRSLPISSLDAGLFFE
                     RPTTWGTHAFTQTLEPRLFYLNVPFRDQSKLPNFTTAETDFNFQRIFSENRFVGGDRI
                     GDANQLTIALTSRMIESETGLERFKAGIGQVYYFQPPRVAINETPTSDKTSDILGFAS
                     SQMSKSVSLDLGWQYTPNLSRSEKVTVGARYSPQPGSILNAAYRYARGIDDPANPSLA
                     GIQQVDVSTQWPIFRGVNALARWNWSLKDRKLLEGLAGFEYNAGCWQVRAVAHRFITA
                     TQTYSTSFQIQLELSGLSRIGINPLETIRQNISGYRRSDEISP"
     gene            1653..2996
                     /locus_tag="VF309_04685"
     CDS             1653..2996
                     /locus_tag="VF309_04685"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7465917.1"
                     /translation="MIFASPRLRLALLAALGAIGLAAAAQGRIDTKLAAPPSSRVVAV
                     DRIVAVVNDEVITQNDLAERVNLVVRQLQRQGGELPSSDTLSRQILERMINDLLQVQL
                     AKESGIKVDDATLDRTIDRIAAENNLSMTDFRAALDKDGVRYPRFREDIRNEILLARL
                     REREVENGIVVTDAEVETELARETREASSESEYRLAHVLVQVPPQASTEQIEQRRRRA
                     VLALSELRRGANFAQIAATYSDAPDALQGGNLGWRPSARLPALFLETIEKLQPGEVSD
                     ILKSANGFHIVKLLEKRGKAAATGVQQTHVRHILLRAKEGLSEGEARDKLQRLRDRIA
                     SGADFAELARINSEDASASKGGDLGWVAPGDTVPEFERVMNALKDNEVSQPVQTPFGW
                     HLVQVLERRSDELSGDRKKLVARQSIRARKADEAFQDWLRQSRDRAFVENRYDER"
     gene            3020..3997
                     /gene="pdxA"
                     /locus_tag="VF309_04690"
     CDS             3020..3997
                     /gene="pdxA"
                     /locus_tag="VF309_04690"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7465918.1"
                     /translation="MGAPLLLVALTAGEPAGIGPDLCALLSREKFPGRLVIVGDRRVI
                     AQRARARGIAFDVPVYRDRDSAPAISVLDVPVRAPVTAGRLERANAAHVVAILDRALD
                     GCVGREFDAMVTAPVQKSILNDAGIPFTGHTEYLAARTGTPLVVMLLVGGGLRVALAT
                     THLALSEVPRAITRELIEAIVRILDRDLRARFGIARPRILVAGLNPHSGESGYLGREE
                     IEVITPAIEALRREGLAVTGPFPADTLFTPRVLETGDVALAMFHDQGLPVLKHAAFGE
                     AVNVTLGLPIIRTSVDHGTALERAGAGDVDAGSLREAIALAFEMAARIP"
BASE COUNT          686 a         1454 c         1261 g          613 t
ORIGIN      
        1 ttcttcctcg gcatgccgat cctctacctt ccgtacatga cgtttccgct cgacaacaag
       61 cgccgctcgg gttttctcgc gccaaccttc ggcacctcgg ggcagagcgg attcgaagcg
      121 tcgctgccgt actactggaa catcgcgccg aacatggacg cgaccatcac gccgaagatc
      181 ttcaccaaga gaggcatcca ggtcgggacg gaattccgct atctctatcc gaagtactcc
      241 ggccagttcg acaccgagta cctgccgaac gacaagatcc gcgagatcga ccgctacttc
      301 ctcggctggc gccacgcgca gcagctgtgg tggcccggct ggagcttcgc ggtgaacgcc
      361 cagaaggtct ccgacgacaa ctacttccgc gacctcacga ccaagatcgc cctcacctcg
      421 cagacgaacc tgccgcgcga cgcgatcctc gcttacaaca acgacgtttg gtcggtgtcg
      481 gcgcgcgtgc tcggctacca gaccctcgag gacccgctga accaggtgcc gattccgtac
      541 aacatcctgc cgcagatcct cgcgttcgga tcgaagcaga acgtgaacgg cttcgactgg
      601 cagctgacga gcgagctttc gaacttccgc cacccgaccc ttcccaacgg ccagcggttc
      661 atcgcgtacc cctcggtctc gctcccgatg cggcaaagct acggctacgt gaccccgaag
      721 atcggcttca acttcacccg ctacaacatg gaccagaaca cgagcggcct cgaaagctcg
      781 agccgctcgc tgccgatatc gagcctggac gcgggactgt tcttcgagcg ccccaccacg
      841 tgggggacgc acgccttcac ccagacgctc gagccgcggc tcttctacct caacgtgccc
      901 ttccgcgacc agtcgaagct gccgaacttc accaccgccg agacggactt caacttccag
      961 cgcatcttct cggagaaccg tttcgtcggc ggcgaccgca tcggcgacgc gaaccagctc
     1021 acgatcgcgc tcacgtcgcg aatgatcgag tcggagacgg gcctcgagcg cttcaaggcg
     1081 ggcatcggcc aggtctacta cttccagccg ccgcgcgtgg cgatcaacga gacgccgacg
     1141 agcgacaaga cctccgacat cctcggcttc gcctcgagcc agatgtcgaa atccgtgtcg
     1201 ctcgacctcg gctggcagta cacgccgaac ctgtcgcgct cggagaaggt cacggtcggc
     1261 gcgcgctact cgccgcagcc gggaagcatc ctgaacgccg cgtaccggta cgcgcgcggg
     1321 atcgacgatc ccgccaatcc gtcgctcgcc ggaatccagc aggtcgacgt ttccacgcag
     1381 tggccgatat ttcgcggcgt gaacgcgctg gcccgctgga actggagcct gaaggaccgc
     1441 aagctcctcg aagggctcgc gggcttcgag tacaatgcgg gctgctggca agttcgcgcg
     1501 gtggcccacc gcttcatcac ggccacgcag acgtattcga cgtccttcca gatccagctc
     1561 gagctctccg gcctctcgag gatcgggatc aatcccctgg aaaccatacg ccagaacatc
     1621 tccggctacc gccggtccga cgagatcagt ccatgatttt cgcttcgccc cgcctccgcc
     1681 tcgccctcct cgccgcgctc ggcgccatcg gcctcgccgc cgccgcgcag ggacgcatcg
     1741 acacgaagct cgccgcgccg ccttcctctc gcgtcgtcgc cgtcgaccgc atcgtagccg
     1801 tcgtgaacga cgaggtcatc acgcagaacg acctcgccga gcgcgtgaac ctcgtcgtac
     1861 gccagctgca gcgccagggc ggggagctgc cctcgtccga cactctctcg cgccagatcc
     1921 tcgagcgcat gatcaacgac ctgctccagg tccagctcgc caaggaaagc ggcatcaagg
     1981 tcgacgacgc gacgctcgac cgcaccatcg accgcatcgc cgccgagaac aatctctcga
     2041 tgacggattt tcgcgcggcg ctcgacaagg acggcgtgcg ctacccgagg ttccgcgagg
     2101 acatccgcaa cgagatcctg ctcgcgaggc tgcgcgagcg cgaagtcgag aacggcatcg
     2161 tggtgaccga cgccgaggtc gagacggaac tcgcgcgcga gacgcgcgag gcctccagcg
     2221 agtcggagta tcggctcgcc cacgttctgg tgcaggtgcc gccgcaggcg agcaccgagc
     2281 agatcgagca gcgccgccgg cgcgcggtgc tcgcgttgtc ggagctgcgc cgcggcgcga
     2341 acttcgcgca gatcgccgcc acctattccg acgcgcccga cgcgctgcag ggcggcaacc
     2401 tcggctggcg cccttccgcg cgcctgcccg cgctgttcct cgaaacgatc gagaagctcc
     2461 agccgggaga ggtgagcgac atcctgaaga gcgccaacgg cttccacatc gtgaagctgc
     2521 tcgagaaacg cggcaaggcg gcggccaccg gcgtgcagca gacccacgtg cgccatatcc
     2581 tgctgcgggc gaaggaaggg ctttccgaag gcgaagcgcg cgacaagctg cagcgcctgc
     2641 gcgatcgcat cgcgagcggc gccgatttcg ccgagctcgc gcgcatcaac tccgaggacg
     2701 cgagcgcctc gaagggcggg gacctcggct gggtggcgcc gggcgacacc gttcccgagt
     2761 tcgagcgcgt gatgaacgcg ctcaaggaca acgaggtgag ccagccggtg cagacgccgt
     2821 tcggatggca cctcgtgcag gtgctcgagc gccgctcgga cgagctttcg ggtgaccgca
     2881 agaagctcgt cgcgcgccag tcgatccgcg cgcgcaaggc cgacgaggcg ttccaggact
     2941 ggctgcgcca gtcgcgcgac cgcgcgttcg tcgagaaccg ctacgacgag cgctagcgcg
     3001 cgcggcggct gaagccgcga tgggcgcccc ccttctcctc gtcgcgctca cggccggtga
     3061 acccgccggc atcggcccgg acctgtgcgc actgctctcg cgcgagaaat tccccggccg
     3121 cctggtgatc gtcggcgacc ggcgcgtgat cgcgcagcgc gcccgggccc gcggcatcgc
     3181 gttcgatgtc ccggtgtatc gcgaccgcga ctccgccccc gcgatctcgg tgctggacgt
     3241 tcccgtgcgg gcgccggtga ccgcggggcg gctcgagcgc gcgaacgctg cgcacgtcgt
     3301 cgcgatcctc gatcgcgccc tcgacggttg cgtcgggcgc gaattcgacg cgatggtgac
     3361 cgcgccagtg cagaagagca tcctcaacga cgccggcatc cctttcaccg gccacaccga
     3421 gtatctcgcc gcgcgcaccg gcacgccgct cgtcgtgatg ctcctcgtgg gcgggggcct
     3481 gcgcgtggcc ctcgccacca cgcacctcgc gctatcggaa gtcccgcgcg cgatcacgcg
     3541 cgagctcatc gaagcgatcg tgcggattct cgatcgcgac ctgcgcgcgc gtttcggcat
     3601 cgcccggccg cgcatcctcg tcgcgggatt gaacccgcat tcgggcgaat cgggatatct
     3661 cggccgggag gaaatcgagg tgatcacacc cgcgatcgag gcgctccggc gcgagggcct
     3721 cgcggtcacc ggacccttcc ccgcggacac gcttttcacg ccacgcgtgc tcgagacggg
     3781 cgatgtagcg ctcgcgatgt tccacgacca gggattgccg gtcctcaagc acgcggcctt
     3841 cggcgaggcg gtcaacgtga cgctgggatt gccgatcatc cgcacttccg tcgatcacgg
     3901 cacggcgctc gagcgcgccg gcgcgggcga cgtcgacgcg gggagcctgc gtgaagcgat
     3961 cgcgctcgcg ttcgagatgg cggcgcgaat cccctaggag cgacagtgct gccc
//
LOCUS       DASVII010000357         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_376425,
            whole genome shotgun sequence.
ACCESSION   DASVII010000357 DASVII010000000
VERSION     DASVII010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376425"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1851)
                     /locus_tag="VF309_04695"
     CDS             complement(<1..>1851)
                     /locus_tag="VF309_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7465919.1"
                     /translation="QGVFPLRNALAMVLGIAADELDLRMDQVAAVAGDTRITPDEGFT
                     SGSFSIEHGGQSVRLAASAARALFLARAATLLNAPRSELSIKEGVVLRRGAATPHTYW
                     SLRDSVDLRVEAARHAAPKAAGERRFSGTSQARIDLPRKVAGSGFIHDLVLPGMRHGR
                     VLHPPSRHARLLTADLAALEAKHGGITTVVNGSFVGLIALRPADAVEAIAEAARRCTW
                     SVGAEAPADPVEAMWTSKSPSVVTFESGDVSAAAGRRFETSVSRPYLAHASIGPSCSV
                     ALWKDDRLTVWSHTQGVFPLRNALAMVLGIAADRIDVIHADGAGCYGHNGADDVALEA
                     ALLARAVPGSPVRVLWSRADELMHSPLGPAAVVKATATVGGDGMLAAMTLDITGQPYG
                     HRPDRDGRANLLAAEYLEGAVAHPPAGDIATGMERNSVPIYAVPSVRVTKRVASELPF
                     RTSSLRSLGAYANVFAIETLVDEIACELGKDPLAFRLAHLKDARARAVLERLAAICGW
                     PGASKEGEGLGIAFARYKNAAAYCAVAARVAADEKDVVVTHMWSVIDAGEVINPDGAV
                     NQIEGGMVQSASWTLKENVQFDGEAIASRGWADYPILTFSETPEVQVEIIA"
BASE COUNT          305 a          654 c          633 g          259 t
ORIGIN      
        1 cgcgatgatc tccacctgga cctcgggcgt ctcgctgaac gtgaggatag gatagtccgc
       61 ccagccgcgg gaagcgatcg cctcgccgtc gaattgcacg ttctccttca gggtccagct
      121 cgccgactgc accatgccgc cctcgatctg gttcacggcg ccgtcgggat tgatcacttc
      181 ccccgcgtcg atcaccgacc acatgtgcgt gacgacgacg tccttctcgt cggcggcgac
      241 gcgcgccgcc acggcgcaat acgccgcggc attcttgtat cgcgcgaagg cgatgcccag
      301 gccctcgccc tccttcgatg cgcccggcca gccgcagatc gccgcgagcc tttccagcac
      361 ggcgcgcgcg cgcgcgtcct tcaggtgcgc gaggcggaac gcgagcggat ccttgccgag
      421 ctcgcaagcg atctcatcca cgagcgtttc gatcgcgaag acattcgcat aggcgcccag
      481 cgagcgcagc gacgacgtgc ggaacggaag ctccgaggcc acgcgcttgg tgacacgcac
      541 gctggggacc gcgtagatgg gaacggagtt gcgctccatc cccgtcgcga tatcgccggc
      601 gggcgggtgc gcgaccgcgc cctcgagata ctccgcggcc aggagattgg cgcggccgtc
      661 gcgatcggga cgatggccgt agggctgacc ggtgatgtcg agcgtcatcg cggcgagcat
      721 gccgtcgccg cccacggtgg cggtggcctt caccacggca gcggggccga gcggcgaatg
      781 catgagctcg tcggcgcggc tccacaggac gcgaactgga gagccgggaa ccgcacgggc
      841 gagcaacgcg gcttcgagcg ccacgtcgtc ggccccgttg tggccgtagc agccggcgcc
      901 gtcggcatgg atgacgtcga tgcgatcggc cgcgatgccc agcaccatcg cgagcgcatt
      961 gcgcagcgga aagacgccct gggtatggct ccacaccgtg agccgatcgt ccttccacaa
     1021 cgcgaccgag cacgacgggc cgatcgaggc gtgcgcgaga tacgggcgcg acaccgacgt
     1081 ctcgaagcgc cggcccgcgg ccgcggacac atcacccgat tcgaacgtga ccaccgaagg
     1141 agacttcgat gtccacatcg cctcgacggg atccgccggc gcctcggcgc ccacgctcca
     1201 cgtgcatcgg cgcgcggcct ccgcgatcgc ctcgaccgca tcggcggggc gcagcgcgat
     1261 caacccgacg aaggaaccgt tcacgaccgt ggtgattccg ccgtgctttg cttcgagcgc
     1321 agcgagatcc gctgtgagaa gtcgcgcgtg gcgcgacggc ggatggagca ctcgcccatg
     1381 gcgcatcccc ggaagcacca ggtcgtggat gaatcccgag cccgcgacct tgcggggcag
     1441 gtcgatcctc gcctgcgaag ttccggaaaa gcgccgttcg cccgccgcct tcggcgccgc
     1501 gtgacgagcc gcttccaccc gaagatcgac ggaatcgcgc agggaccaat acgtgtgcgg
     1561 cgtcgcggct ccgcggcgca gcaccacgcc ttccttgatg gagagctccg agcgcggcgc
     1621 gttcaagaga gtcgcggcgc gggcgaggaa cagcgcgcgc gcggccgaag cggcgagacg
     1681 caccgattgc ccgccgtgct cgatcgaaaa gcttcccgag gtgaagcctt cgtcgggcgt
     1741 gatgcgggtg tcgccggcga ccgccgcgac ctggtccatg cgcaggtcga gctcatcggc
     1801 cgcgatgccc agcaccatcg cgagcgcatt gcgcagcgga aagacgccct g
//
LOCUS       DASVII010000358         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_412242,
            whole genome shotgun sequence.
ACCESSION   DASVII010000358 DASVII010000000
VERSION     DASVII010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412242"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..542
                     /gene="tolR"
                     /locus_tag="VF309_04700"
     CDS             132..542
                     /gene="tolR"
                     /locus_tag="VF309_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX7465920.1"
                     /translation="MKRTRRAMAEINVVPYVDVMLVLLIIFMVAAPLINPGQIELPQA
                     AARLEPAVAPMEVRVRANGDLALVDRNKSSDEVRVTRDELLDFVRQAQQSHPDQAIVI
                     AGDRAVRYEEVIKVLDLLQRNQVKRVGLLARPAQ"
     gene            539..1378
                     /locus_tag="VF309_04705"
     CDS             539..1378
                     /locus_tag="VF309_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7465921.1"
                     /translation="MKQKKAAAHPIEKPPQPGRIRAVFLAVLVHAAFFALIVFGVAWQ
                     SRPDVPVIAELWDKLPPAPTAKTVEPAPKAEPKAEPPKPEPEPPKPKPVKPPPTPPDE
                     KPEPPKPDPAIAKKLEREKKELEKKEKLEREKLQKQKDDEAKKKREKEETEKKKREEE
                     ARRKEDERARAEAQKAREQAAAAQKSEIDRFINDIKNKIRGKANVPDTVTGNPEVHVR
                     IRVLPGGEVLDITVTKPSGNPTYDAAIERGIRSASPLPVPAPNSELFPQFRDLNLNFR
                     HER"
     gene            1401..>1609
                     /locus_tag="VF309_04710"
     CDS             1401..>1609
                     /locus_tag="VF309_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEX7465922.1"
                     /translation="MTRYLLLLVALAFSLAARAQLTIDITTSGGRQVPIAITPMAGDS
                     SQPQSVSDVIGADLARTGLFRLVNTA"
BASE COUNT          323 a          562 c          520 g          204 t
ORIGIN      
        1 tacaaccgct acacccacga catcgaccgc ctcgcgagcc gctacgagag cttcatggag
       61 gagctgcaga acatcctcca gcggcaggcg caccttccgg acgcctcgga cacggtcgtg
      121 cagaggccgc gatgaagcgc acgcgccgcg cgatggccga gatcaacgtc gtgccgtacg
      181 tcgacgtgat gctggtgctc ctcatcatct tcatggtggc cgcgcccctc atcaacccgg
      241 ggcagatcga gctgccccag gcggcggcgc gcctcgagcc cgcggtcgcg ccgatggagg
      301 tgcgcgtgcg cgcgaacggc gacctcgcgc tcgtcgaccg caacaagtcg tccgacgagg
      361 tgcgcgtcac ccgcgacgag cttctcgact tcgtgcgcca ggcgcagcaa tcgcatcccg
      421 accaggcgat cgtgatcgcg ggcgaccgcg cggtgcgcta cgaagaggtg atcaaggtcc
      481 tcgacctgct gcagagaaac caggtgaagc gcgtggggct gctcgctcgc ccggcccagt
      541 gaagcagaag aaggcggcgg cgcacccgat cgagaagccc ccgcagccgg ggcgcatccg
      601 cgccgtgttt ctcgcggtgt tggtgcacgc cgcgttcttc gcgctgatcg tcttcggcgt
      661 cgcgtggcaa agccgccccg acgtgcccgt gatcgcggag ctctgggaca agctgccgcc
      721 cgcgcccacc gcgaagacgg tagagcccgc gccgaaagcc gagcccaagg cggagccgcc
      781 gaagcccgag cccgagccgc cgaagcccaa gcccgtcaag cctcctccga cgccgccgga
      841 cgagaagccc gagccgccca agcccgatcc cgcgatcgcg aagaagctcg agcgcgagaa
      901 gaaggagctc gagaagaagg aaaagctcga gcgcgagaag cttcagaagc agaaggacga
      961 cgaggcgaag aagaagcgcg agaaggaaga gacggaaaag aaaaagcgcg aggaagaggc
     1021 gcgccgcaag gaagacgagc gcgccagggc cgaggcgcag aaggcgcgcg agcaggccgc
     1081 tgcggcgcag aaatccgaga tcgatcgttt catcaacgac atcaagaaca agatccgcgg
     1141 caaggccaac gtgcccgaca cggtcacggg caaccccgag gtccacgtgc gcatccgcgt
     1201 gctccctggc ggcgaggtcc tggacatcac ggtcaccaag ccgagcggca atcccacgta
     1261 tgatgccgcc atcgaaaggg gcatccggag cgcttcgccg ttaccggttc ccgcaccaaa
     1321 ttcggaactt ttccctcagt ttcgggacct taacctgaac ttcaggcatg aacgctagcc
     1381 tagacccctc ctcgacaccc atgacgcgtt acctcctttt gctggtggcg ctggcgtttt
     1441 ccctcgccgc gcgcgcccag ctcaccatcg acatcaccac cagcggcggg cgccaggtgc
     1501 cgatcgccat cacgcccatg gccggcgact cgtcgcagcc gcagtcggtg agcgacgtga
     1561 tcggcgcgga cctcgcgcgc accgggctct tccgcctggt caacacggc
//
LOCUS       DASVII010000359         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_196065,
            whole genome shotgun sequence.
ACCESSION   DASVII010000359 DASVII010000000
VERSION     DASVII010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196065"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VF309_04715"
     CDS             complement(<1..781)
                     /locus_tag="VF309_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7465923.1"
                     /translation="MAAYGLEEKGRRSVYVIDGARTPFLKAKTGLGPFTGSDLAVNAG
                     RALLMRQPFEPDAFDEVIVGAAMPSADEANIARVVSLRLGCGDKVPAWTVMRNCASGL
                     QALDSAAVNIIAGRSSLVLAGGTDAMSHAPLLYGPAMVNWLAAWYAAKSMPQKAVLAA
                     RFRLAYLAPVIAILRGLTDPIVKISMGSTAEIVAKRFGITRQMMDEFAVESHRRTAFA
                     QDNGHLTEVEPMYGPDGTVYAQDDGVRRDSSVERLAKLKPVF"
     gene            complement(733..>1803)
                     /locus_tag="VF309_04720"
     CDS             complement(733..>1803)
                     /locus_tag="VF309_04720"
                     /inference="COORDINATES: protein motif:HMM:NF020876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX7465924.1"
                     /translation="MDIHGGKGIMMGPNNYLGSAYQSIPIAITVEGANILTRSLIIFG
                     QGAIRCHPFVLKEIAAAGANDLGAFDRALFGHMAFTISNAARSLYLGITGGETVTVAG
                     PPETRRYLQMMTRFSSAFALLSDISMFVIGGSLKRREKISARLGDILSLLYIASATVK
                     RFNDEGRQKADRPLLDWAMFDTFFKLQVAMDGVLDNFPNRWVAAWMRMLVFPKGLTLN
                     APFDKVGAKVAAILITPGPARDRLTAGAYIPRDVNDVIGRLEFAMEAVVKAETIEAKM
                     RKATKEGLLTQRTVEERRTVALAQGIITQADVEHLEYTDRLRRDVVKVDDFENDLGRG
                     AKSQEEPWQPTDSKKKVAEASM"
BASE COUNT          282 a          615 c          596 g          310 t
ORIGIN      
        1 cgaacacggg cttcagcttc gcgagcctct ccacgctgga atcccgtcgc acgccgtcat
       61 cctgcgcata cacggtgccg tcgggtccat acatcggctc gacttccgtg agatggccgt
      121 tgtcctgcgc gaacgccgtg cgccggtgcg attcgaccgc gaattcgtcc atcatctgtc
      181 gcgtgatgcc gaagcgcttc gcgacgatct ctgcggtgga tcccatggag atcttcacga
      241 tgggatcggt gaggccgcgc aggatcgcga tcaccggcgc gaggtacgcc agccggaagc
      301 gcgccgccag caccgccttc tgcggcatcg acttcgccgc ataccacgcg gcgagccagt
      361 tcaccatcgc cggcccgtag aggagcggcg cgtggctcat ggcgtcggtg ccgccggcga
      421 gcaccaggct cgagcgtccc gcgatgatgt tcaccgccgc ggaatcgagc gcctgcagcc
      481 ccgaggcgca gttgcgcatc acggtccatg cgggcacctt gtcgccgcag ccgagccgca
      541 gcgacacgac gcgcgcgatg ttcgcctcgt ccgccgacgg catcgccgcg ccgacgatca
      601 cttcgtcgaa ggcgtcgggt tcgaacggct ggcgcatgag gagcgcccgc cccgcgttca
      661 ccgcaaggtc cgagcccgtg aacggcccca gccccgtctt cgccttgagg aacggggtgc
      721 gagccccgtc gatcacatag acgcttcggc gacctttttc ttcgagtccg taggctgcca
      781 tggctcctcc tgcgatttcg cgccgcgccc gaggtcgttc tcgaagtcgt ccaccttgac
      841 gacgtcgcgg cgcaggcgat cggtgtattc gagatgctcg acgtcggcct gggtgatgat
      901 tccctgcgcg agcgccaccg tgcggcgctc ctccaccgtg cgctgcgtga ggaggccttc
      961 cttcgtcgcc ttgcgcatct tcgcctcgat ggtctcggcc ttcaccacgg cctccatcgc
     1021 gaattccagc cggccgatga cgtcgttcac gtcgcgcggg atgtacgcgc cggccgtgag
     1081 gcgatcgcgc gcggggcccg gcgtgatgag gatcgccgcg accttggcgc ccaccttgtc
     1141 gaacggcgcg ttcagcgtga gccctttcgg gaacacgagc atgcgcatcc aggcggcgac
     1201 ccagcgattg gggaagttgt cgagcacgcc gtccatcgcg acctgcagct tgaagaacgt
     1261 gtcgaacatc gcccagtcga ggagcggccg gtccgccttc tggcggccct cgtcgttgaa
     1321 gcgcttcacc gtcgccgagg cgatgtagag aagcgagagg atgtcgccca ggcgcgcgga
     1381 gatcttctcg cgtcgcttga ggctcccccc gatcacgaac atcgagatgt ccgagagaag
     1441 cgcgaaggcc gacgagaagc gcgtcatcat ctgcaggtag cggcgcgtct cgggcgggcc
     1501 cgccaccgtg accgtctcgc cgccggtgat gcccagatac agcgagcgcg cggcattgga
     1561 gatcgtgaag gccatgtgcc caaacagcgc gcggtcgaag gcacccaggt cattggcgcc
     1621 cgcggcggcg atctccttca gcacgaaggg atggcagcgg atcgcgccct gtccgaagat
     1681 gatgagggag cgcgtaagga tgttggcgcc ctccacggtg atcgcgatcg ggatcgactg
     1741 gtaggcgctg ccgagatagt tgttcggccc catcatgatg cccttgccgc cgtggatgtc
     1801 cat
//
LOCUS       DASVII010000360         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_160445,
            whole genome shotgun sequence.
ACCESSION   DASVII010000360 DASVII010000000
VERSION     DASVII010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160445"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VF309_04725"
     CDS             complement(<1..449)
                     /locus_tag="VF309_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465925.1"
                     /translation="MAIIETLEAALKPWADFYGHNKMVAAGVEFMHVGALLVGGGFAI
                     ASDRAALRALKAPIEHQKHVLKEFTTVHKPVIIALSIMVISGLLMLTSDIGTFLVSPV
                     YWTKMTLVTLLLINGYLVKRADEKLNADPSPGNRAWKHFKFSSIASIT"
     gene            495..>2235
                     /locus_tag="VF309_04730"
     CDS             495..>2235
                     /locus_tag="VF309_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7465926.1"
                     /translation="MSLLSLSDIAVEFGATKLFDRVTFTVGKGDRWGIVGRNGSGKTS
                     LFKIITGTLTPIRGAVARMPGLKVSLLDQHRDFGGAVTVWDAAALPYAPVLALEVSLA
                     EQAAQLAELGERAPAALLQQYGHDLETFAHEGGYTFHARVDAVLQGLAFDAEESKTRR
                     VATLSGGERGRVGLAAQLVAPADLLLLDEPTNHLDLETTAWLQEYLRELDETVIVISH
                     DRAFLDEIADHVLHLEGGTAETYRGGYSSFVQQRAERRLALERQVDQQRKMIAKEEDY
                     IRRNIAGQNTSQAKGRRKRLDRLPRLTPPPGSGGTMELRLEISERGGDRVIAAEGLTV
                     AIGDRTLVRDFSATANRGDVIALVGPNGAGKTTLIKTLLGEREAQGGAVKLGGSVSPA
                     YFRQDHDKLPLDSSLYEVIENLRPLWTRGGIQNHLGCFGFSGEEVFRSTRTLSGGERS
                     RLALAVIVLQRANMLVLDEPTNHLDVESIEAIEDAIEEYEGTVLLVSHDRALLRELAT
                     RVWAFDGDRLVDFGGTFVDWERQLRDAALAKRATNAVASDARREQEKVRARGNAAAEQ
                     KEQAARRAAKKTAD"
BASE COUNT          392 a          753 c          733 g          357 t
ORIGIN      
        1 gtgatactcg cgatggaact gaacttgaag tgcttccacg cgcggttgcc gggggagggg
       61 tcggcgttga gcttttcgtc cgcgcgcttg actaaatacc cgttgatgag caaaagcgtc
      121 accagcgtca tcttcgtcca atacacgggc gagacgagga acgttccgat gtcgctcgtg
      181 agcatcagca gccccgagat gaccatgatg ctgagcgcga tgatcacggg tttgtggacg
      241 gtcgtgaact ctttcagtac gtgcttctga tgttcgatcg gcgccttcag cgcgcgaagc
      301 gccgcgcgat ccgaggcgat cgcgaatccg ccgcccacga gcagcgcgcc cacatgcatg
      361 aactcgacgc cggccgccac catcttgttg tgcccgtaga aatctgccca cggtttcagg
      421 gccgcctcga gcgtctcgat aattgccatc gctcagcact ccaaataaag gtgtccgcca
      481 gcgatctttc gtgaatgtcc ctgctctcgc tttccgacat cgccgtcgaa ttcggcgcga
      541 ccaaactttt cgaccgtgtg acgttcacgg tcgggaaggg tgatcgctgg ggaattgtcg
      601 ggcgcaatgg cagcggcaag acgtcgttgt tcaagataat caccggcact ctcacgccga
      661 ttcgcggcgc ggtggcgcgc atgccggggc tcaaggtctc cctgctcgac cagcaccgcg
      721 atttcggcgg ggcggtcacc gtgtgggacg cggcggctct tccgtacgcg ccggtcctgg
      781 cgctcgaagt gtcgctcgcc gagcaggcgg cgcagctcgc cgaactcggt gagcgcgcgc
      841 ccgccgccct gctccagcag tacgggcacg atctcgagac gttcgcgcac gagggcggct
      901 acacctttca cgcacgcgtg gacgccgtgc tgcagggcct cgcgttcgac gccgaggaat
      961 ccaagacccg gcgcgtggcc acgctgagcg gcggtgagcg cggccgcgtc gggctcgccg
     1021 cgcagctcgt cgcgcccgcg gatctcctgc tgctcgacga gcccacgaac catctcgatc
     1081 tcgagacgac cgcgtggctg caggaatacc tgcgcgagct cgacgaaacg gtgatcgtca
     1141 tcagccacga ccgcgcgttt ctcgacgaga tcgccgacca cgtgctgcat ctcgagggcg
     1201 gcaccgcgga aacctatcgc ggcggttact ccagcttcgt gcagcagcgc gccgaacgcc
     1261 gcctcgcgct cgagcggcag gtggatcagc agcggaagat gatcgcgaag gaagaggact
     1321 acatccgccg caacatcgcc ggacagaaca cgtcgcaggc gaagggacgc cgcaagcggc
     1381 tcgaccgcct tccgcgcctc acgccgccgc cgggatcggg cggcacgatg gagctccgtc
     1441 tggagatttc cgagcgcggc ggcgatcgcg tgatcgccgc cgagggactc accgtcgcga
     1501 taggcgaccg cacgctcgtg cgcgacttct ccgccaccgc gaatcgcggc gacgtgatcg
     1561 cgctcgtcgg accgaacggc gccggaaaga ccacgctcat caagacgctg ctcggcgagc
     1621 gcgaggcgca gggcggcgcg gtgaagctcg gcggttccgt ctcccccgcg tacttccggc
     1681 aggaccacga caagcttccg ctcgactcga gcttgtacga agtgatcgag aatctccgtc
     1741 cgctctggac gcgcggcgga attcaaaatc acctcggctg cttcggattc tccggcgagg
     1801 aagtcttccg ctccacgcgc acgctttccg gcggcgagcg gtcgcgcctc gcgctcgccg
     1861 tgatcgtgct gcagcgcgcg aacatgctcg tgctcgacga gcccacgaac cacctcgacg
     1921 tcgaatcgat cgaagccatc gaggacgcga tcgaggaata cgagggaacc gtgctgctcg
     1981 tcagccatga ccgcgcgctg ctccgcgagc tcgccacgcg cgtatgggcc ttcgacggtg
     2041 accggctcgt ggatttcggc ggcacgttcg tggactggga acgccagctg cgcgatgcgg
     2101 cgctcgcgaa acgcgccacc aacgccgtcg cgtcggacgc gaggcgcgag caggagaaag
     2161 ttcgcgcgcg cggaaacgcc gccgcggagc agaaggagca ggccgcgcgc cgcgccgcga
     2221 agaagaccgc cgaca
//
LOCUS       DASVII010000361         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_88491, whole
            genome shotgun sequence.
ACCESSION   DASVII010000361 DASVII010000000
VERSION     DASVII010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88491"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="VF309_04735"
     CDS             complement(<1..1231)
                     /locus_tag="VF309_04735"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX7465927.1"
                     /translation="MKTLQATLLGVLIVVGIAAQVPNITRAQSPAAAPAPAAPARIAT
                     PSTPGASAQILPDFADLVEKYGPAVVNINTQTRAAARPQLPGLSEDDPFYEFFRRFMP
                     PEGRNNPSPNAHGNRYREPDAAPKGPLRPFGLGSGFIVSPDGFIVTNAHVVENAEELT
                     VRLTDKREFKAKVIGVDTRSDVAVVKIDATSLPTVKIGDMSKVRVGEWVIAIGSPFGF
                     ANTVTAGIVSAKSRENLSGDPNTDAVPFIQTDVAVNPGNSGGPLLNMRGEVVGINSQI
                     FSRTGSFAGISFAIPIDYAFNVADQIMKTGHVTRGRIGVSIQTVSRDLADSLGRGKAD
                     GAVVGSVEADSPASRAGLEAGDIITKIDGRGVEGSADLSRTIRSLKPGSKVMLTVWRS
                     GKTHDFPATITEFKDEDT"
     gene            complement(1334..>2039)
                     /locus_tag="VF309_04740"
     CDS             complement(1334..>2039)
                     /locus_tag="VF309_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX7465928.1"
                     /translation="EMFCYFPDAKTVRLDRRVTARFFPAVVSGPSAMVAANYEVTLGP
                     TERMLGYDCRWIRLDPRDALRYAQRLCSELNTGLVIRAKTLNEQQQVIEQYTFTDLKL
                     GAQVAKSDVKSIFEARVKRWITDARPREEARGAETGWIVASPPAGFQKINELKRTLPG
                     HGAPVSQIVYSDGVASLSVFVEPNNGPAPRTAEASTEDGTTTFFVRPMGEQLVSVLGE
                     VPLATAQQFGRNVARR"
BASE COUNT          301 a          663 c          713 g          362 t
ORIGIN      
        1 tcgtgtcctc gtccttgaat tccgtgatcg tcgccgggaa gtcgtgcgtc ttgccgctgc
       61 gccacacggt gagcatcacc ttgctgccgg gcttcaggga gcggatggtg cgcgacaggt
      121 ccgccgagcc ttccacgccg cgcccgtcga tcttcgtgat gatgtcgccc gcttccaggc
      181 ccgccctgga cgccggggaa tcggcctcga ccgagcccac caccgcgccg tcggccttgc
      241 cgcgcccgag gctgtcggcg aggtcgcggc tcacggtctg gatgctcacg ccgatgcgcc
      301 cgcgcgtgac gtggcccgtc ttcatgatct ggtcggcgac gttgaaggcg tagtcgatcg
      361 ggatcgcgaa cgagatgccc gcgaagctgc cggtgcgcga gaagatctgc gaattgatgc
      421 cgaccacctc gccgcgcatg ttgagcagcg gcccgccgga gttgcccgga ttcaccgcga
      481 cgtcggtctg gatgaaggga accgcatcag tgttcggatc gcccgaaagg ttctcgcggc
      541 tcttcgcgct cacgatgccc gcggtgacgg tattcgcgaa tccgaagggc gagccgatgg
      601 cgatcaccca ttcgcccacg cgcaccttgc tcatgtcgcc gatcttcacg gtgggaaggc
      661 tcgtcgcgtc gatcttgacc acggccacgt cgcttcgggt gtcgacgccg atcaccttcg
      721 ccttgaactc gcgcttgtcg gtgaggcgca cggtgagctc ttccgcgttc tcgaccacgt
      781 gcgcgttggt gacgatgaat ccgtcgggcg agacgatgaa tcccgagccc aggccgaacg
      841 ggcgcagcgg gcccttgggg gccgcgtcag gctcgcggta gcgattgccg tgcgcgttgg
      901 gcgacggatt gttgcggccc tccggcggca tgaagcggcg gaagaactcg tagaacggat
      961 cgtcctccga cagccccgga agctgcgggc gcgccgcggc gcgcgtctgc gtgttgatgt
     1021 tgaccaccgc cgggccgtat ttctcgacga ggtcggcgaa atcgggaagg atctgcgccg
     1081 aggcgccggg agtgctcggg gtggcgatgc gagccggggc cgccggcgcc ggcgcggcgg
     1141 ccggggattg cgcccgggtg atattgggaa cctgggccgc gatgccgact acgatcagca
     1201 cgccgagaag ggttgcctgc agggttttca tgatggtgcc tcggtcctgt tctatgggct
     1261 tgcgccggcg ggggctcgat tcaatcagat gggggcccgc ggaagtgatt cccagtgcaa
     1321 gagtaacgaa atttcagcgc cgcgcgacgt ttcggccgaa ctgctgcgcc gtcgcgagcg
     1381 gcacctcgcc cagcaccgac accagctgct cccccatggg gcgcacgaaa aacgtggtgg
     1441 tgccgtcttc ggtcgaggct tcggcggtgc gcggcgcggg gccgttgttg ggctccacga
     1501 acaccgaaag gctcgccacg ccgtcactgt agacgatctg cgaaaccggc gcgccgtggc
     1561 cgggcagcgt gcgcttgagc tcgttgatct tctggaatcc ggcgggcggg ctcgcgacga
     1621 tccagccggt ctccgcgccg cgcgcttcct cgcggggccg ggcgtcggtg atccagcgct
     1681 tcacgcgcgc ctcgaagatc gatttcacgt cgctcttggc gacctgcgcg cccagcttga
     1741 ggtcggtaaa ggtgtattgc tcgatcacct gctgctgctc gttcagggtt ttcgcccgga
     1801 tgacgaggcc cgtattcagc tcggagcaga ggcgttgcgc gtagcgaagc gcatcccgcg
     1861 gatcgagccg aatccagcgg caatcgtagc cgagcattcg ctcggtcggg ccgagcgtca
     1921 cctcgtagtt cgcggccacc atcgccgacg gcccggagac gaccgcggga aagaaccgcg
     1981 ccgtgacgcg tcggtcgagc cgcaccgttt tcgcgtcggg gaagtagcag aacatctcc
//
LOCUS       DASVII010000362         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_556487,
            whole genome shotgun sequence.
ACCESSION   DASVII010000362 DASVII010000000
VERSION     DASVII010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556487"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..780)
                     /locus_tag="VF309_04745"
     CDS             complement(13..780)
                     /locus_tag="VF309_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465929.1"
                     /translation="MSGALRTAGLSKRFGGLSAVSDVSLEIPEGARHALIGPNGAGKT
                     TLINLLTGVLEPSAGDVFLGGERITALPQHLRVKKGLTRTFQINTLFPGLTVLESVVL
                     AILERKGLAARWMRPVSSHRAEIGEATELLATFKLDRDAQRRTAELAYGRQRLVEIAL
                     ALATRPKILLLDEPAAGIPSAESVEVLGVIAALPADVTVLFIEHDMGLVFRFARRITV
                     LVAGRVLIEGTPDEIARDPQVKAVYLGKDADEARDGR"
     gene            complement(777..1757)
                     /locus_tag="VF309_04750"
     CDS             complement(777..1757)
                     /locus_tag="VF309_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7465930.1"
                     /translation="MSALPGALRGAKLIEVAFWLVPLAAFLLLPGYLVMGSQILIGAL
                     FALSLDLILGYAGIVSLGHAAFFGLGAYAAGLLAVNGWGEPLSGLLAGGAIAALAGFV
                     ASFLVVRGEDLARLMVTLGIGLLLYEAANRAAFLTGGVDGLSGVVMGKIFGVFAFDIG
                     GKTAYLYSFAVLLLVFIFLKRLTSSPFGLSLRGIRENPARMPAIGSPVRARLVTAYTI
                     GAAIAGIAGALLAQTTQFVGLDSLGFNRSADLMIMLVLGGTGRLYGAIIGAAVFMIAQ
                     DYLSGVNPVYWQFWLGLLLVVFVFFARGGILGAAEAAAKRLGRSSRERAR"
     gene            complement(1754..>2553)
                     /locus_tag="VF309_04755"
     CDS             complement(1754..>2553)
                     /locus_tag="VF309_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7465931.1"
                     /translation="LGLSVTMGLMNFVNLAHGAFAMAGGYVCVIAMTRFGVPFLATLP
                     LAFVVIAIVGAILERTLYRRLYGANHLDQVLFSIGLTFMSIAAATYFFGSSQQPMRLP
                     EFLRGQVHLGGVDFGIYRLFLVAVVAALTAVLGFLLARTRFGAQVRASVDNAQAARGL
                     GIDVDRVFSVAFALGSGLAGLGGALGIEVLGLDSSFPLKFMVYFLLVVVVGGAGTVKG
                     PLVAALLLGVLDVAGKYYVPQVGAFVIYAAMVILLIAFPAGLFGRRR"
BASE COUNT          491 a          880 c          887 g          295 t
ORIGIN      
        1 aggcgcagga gctcagcgcc catcccgcgc ctcgtcggca tccttgccga gatagaccgc
       61 ctttacctgc ggatcgcgcg cgatctcgtc gggcgtgccc tcgatcagga cgcgcccggc
      121 caccagcacc gtgatgcgcc gcgcgaagcg gaacacgagg cccatgtcgt gctcgatgaa
      181 gagcacggtc acgtccgcgg gaagagccgc gatcacgccg agcacctcga cgctttcggc
      241 cgacggaatt cccgccgccg gctcgtcgag gagcaggatc ttcggccgcg tcgcgagcgc
      301 gagcgcgatc tcgaccaggc gctggcgccc gtacgcgagc tcggcggtgc ggcgctgcgc
      361 atcgcgatcg agcttgaagg tcgcgagcag ctccgtcgcc tcgccgattt ccgcacggtg
      421 actcgacacc gggcgcatcc agcgcgcggc gaggcccttg cgctcgagga tcgcgagcac
      481 cacggactcg agcaccgtca gccccgggaa gagcgtgttg atctggaagg tgcgcgtgag
      541 ccccttcttc acgcgaagat gctgcggcag cgccgtgatg cgctcgccgc cgaggaagac
      601 gtcgcccgcg ctgggctcga gcacaccggt cagcaggttg atgagcgtcg tctttcccgc
      661 gccgttgggg ccgatcagcg catggcgcgc gccctcgggg atctcgaggc tcacgtcgct
      721 cacggcgctg aggccgccga agcgcttgga gagaccggcc gtacgcagcg cgccgctcat
      781 cgcgcgcgct cccgcgagga gcgtccgagc ctcttcgcgg cggcctcggc cgcgccgagg
      841 attccgccgc gcgcgaaaaa aacgaacacg acgagcagca gcccgagcca gaactgccag
      901 tagacggggt tcactcccga aaggtagtcc tgcgcgatca tgaacaccgc ggcgccgatg
      961 atcgcgccgt agagccggcc ggtgccgccc agcaccagca tgatcatgag atccgccgag
     1021 cggttgaagc cgagcgagtc gaggcccacg aactgggtcg tctgcgcgag gagcgcgccc
     1081 gcgatccccg cgatcgccgc gccgatggtg tacgcggtga cgagcctcgc gcgcaccggc
     1141 gagccgatgg cgggcatgcg cgcgggattc tcgcggatgc cgcgaagcga aagcccgaag
     1201 ggcgagctcg tgagccgctt caggaagatg aacaccagca ggagaaccgc gaagctgtag
     1261 aggtacgccg tctttccgcc gatgtcgaag gcgaagacgc cgaagatctt tcccatcacg
     1321 acgcccgaga gcccgtcgac gccgcccgtg aggaacgccg cgcggttggc ggcctcgtag
     1381 aggagaagcc cgatcccgag cgtgaccatc aggcgcgcga gatcctcgcc gcgcacgacg
     1441 aggaagctcg ccacgaaccc ggcgagcgcg gcgatcgcgc cgcccgcgag aaggccgctc
     1501 aagggctcgc cccacccgtt cactgcgaga aggcccgcgg cataggcgcc gagcccgaag
     1561 aacgcggcgt ggccgaggga gacgatgccc gcgtagccca ggatgaggtc gagcgatagc
     1621 gcgaagagcg cgccgatgag gatctggctt cccatcacca ggtagccggg caggagaagg
     1681 aacgcggcga ggggcaccag ccagaaggcg acctcgatca gcttcgcgcc gcgaagggcg
     1741 cccggaagag cgctcaacgg cgcctcccga agagccccgc ggggaacgcg atgagcagga
     1801 tcaccatcgc cgcgtagatc acgaatgcgc ccacttgggg cacgtagtac ttgcccgcca
     1861 cgtcgagcac tccgagaagc agcgcagcga ccagcgggcc cttgacggtg cccgcgccgc
     1921 cgaccacgac gaccagcagg aaatacacca tgaacttgag cggaaacgag gaatcgaggc
     1981 cgagcacctc gatgccgagc gcgccgccca atccggcgag ccccgagccc agcgcgaagg
     2041 cgacgctgaa gacgcggtcc acgtcgatgc cgagcccgcg cgcggcctgc gcgttgtcga
     2101 ccgaagcgcg cacctgcgcg ccaaagcgcg tgcgcgcgag gaggaatccg agcaccgccg
     2161 tgagagccgc gacgaccgcg acgaggaaca gccgatagat gccgaagtcc acgccgccga
     2221 ggtgcacctg cccgcggagg aattccggaa gccgcatcgg ctgctgggag gagccgaaga
     2281 aatacgtcgc cgccgcgatc gacatgaacg tgaggccgat cgagaaaagg acctggtcca
     2341 ggtggttcgc gccatagagg cgccggtaga gcgtgcgttc caggatcgca cccacgatgg
     2401 cgatgaccac gaacgccagc ggcagcgtcg cgaggaacgg cacgccgaag cgcgtcatcg
     2461 cgatcacgca gacatagccg cccgccatgg cgaacgcgcc atgggcgagg ttcacgaagt
     2521 tcatgaggcc catcgtgacc gaaaggccca ggc
//
LOCUS       DASVII010000363         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_52999, whole
            genome shotgun sequence.
ACCESSION   DASVII010000363 DASVII010000000
VERSION     DASVII010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52999"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /gene="carB"
                     /locus_tag="VF309_04760"
     CDS             <1..580
                     /gene="carB"
                     /locus_tag="VF309_04760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986498.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX7465932.1"
                     /translation="VPPYFSVKEAVFPFIKFPGVDTILGPEMKSTGEVMGVGQTFAEA
                     FVKSQLAAGVRLPKGGKVFLSVRNEDKERLVDIARDLVDLGFSLVATRGTAATLEKAG
                     LKVQPVNKVQEGRPHIVDMLKNREVSLIVNTVEEDRRSVQDSWSIRNSALQNRITYYT
                     TLAGARAACVGMRHLEEMRPYDIQSLHAQLSS"
     gene            577..1053
                     /gene="greA"
                     /locus_tag="VF309_04765"
     CDS             577..1053
                     /gene="greA"
                     /locus_tag="VF309_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7465933.1"
                     /translation="MNKIPITMIGAELLRNELHKLKTVERHAVIAAIAEARAHGDITE
                     NAEYEAARERQGFIEGRIIELEAKLSNCQIIDPALLDDDGRVVFGSTVELIDTSGKET
                     VSYQIVGEDEADIKLGKISYSSPIAKALIGKSEGDVVEVRTPGGLKEFEIGDVRYV"
     gene            complement(965..1426)
                     /gene="yhbY"
                     /locus_tag="VF309_04770"
     CDS             complement(965..1426)
                     /gene="yhbY"
                     /locus_tag="VF309_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEX7465934.1"
                     /translation="MPLNPSRRSELRAQAHTLSPVVIIGDKGLTDEVVAEIDRSLKAH
                     ELIKVRAATDDRGAREEWMKAICEKLGAHPVQKIGKVLVLYRENPKERIREVRETPRP
                     AKPAPRGKPTLRKSRVAQAASESRRTERPRSRTPSARPESEPRRRRPRSSR"
     gene            complement(1512..1988)
                     /gene="greA"
                     /locus_tag="VF309_04775"
     CDS             complement(1512..1988)
                     /gene="greA"
                     /locus_tag="VF309_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7465935.1"
                     /translation="MSKIPITMIGAELLRNELHKLKTVERHAVITAIAEARAHGDITE
                     NAEYEAARERQGFIEGRIIEVEAKLSNCQIIDPALLDDDGRVVFASTVELIDTKGGAT
                     VSYQIVGEDEADIKLGKISYSSPIAKALIGKSEGDVVEVRTPGGLKEFEIGDVKYL"
     gene            complement(1985..>2788)
                     /gene="carB"
                     /locus_tag="VF309_04780"
     CDS             complement(1985..>2788)
                     /gene="carB"
                     /locus_tag="VF309_04780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206292.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX7465936.1"
                     /translation="VVGLMNVQFAIQNAQNAQTAGAGHEIYVLEVNPRASRTVPFVGK
                     ATGLPLAKIAARCMVGQSLAKQGATKEVIPPYYSVKEAVFPFIKFPGVDTILGPEMKS
                     TGEVMGVGHTFAEAFVKSQLAAGVKLPRGGKVFLSVRDEDKERLVEIARDLADLGFAL
                     VATPGTAASLEKAGLKVQPVNKVQEGRPHIVDMLKNREVSLIVNTVEEDRRSIQDSWS
                     IRNSALQNRITYYTTLAGARAACVGMRHLEEMRPYDIQSLHASLPTCPT"
BASE COUNT          460 a          918 c          901 g          509 t
ORIGIN      
        1 cgtgccaccg tatttttcgg tgaaggaagc cgtgttcccg tttatcaaat ttccgggcgt
       61 cgacaccatc cttggcccgg agatgaaatc cacgggcgag gtgatgggcg tgggccagac
      121 tttcgccgaa gcgttcgtga agagccagct tgccgcgggc gtgaggctgc ccaagggagg
      181 caaggtgttc ctctcggttc gcaacgagga caaggagcgc ctcgtcgaca tcgcgcgcga
      241 cctcgtcgac ctcggcttct cgctggtggc gacgcggggg acggcggcga cgctggagaa
      301 ggccggcctc aaggtgcagc ccgtgaacaa ggtccaggaa ggccgtccgc acatcgtaga
      361 catgctgaag aaccgcgaag tgagccttat cgtgaacacc gtcgaggaag accgccgctc
      421 cgtccaggac tcttggtcga tccgcaattc ggcgctgcag aaccgcatca cgtactacac
      481 gactctcgcg ggagcgcgcg cggcctgcgt cggcatgcgc cacctcgagg aaatgcgccc
      541 ctacgacatc caatcgctgc acgcccaact gagctcatga acaagatccc catcaccatg
      601 atcggcgccg agctgttgcg caacgagctc cacaagctga agaccgtgga gcgccacgcg
      661 gtgatcgccg ccatcgccga ggcgcgagcc catggcgaca tcaccgagaa cgcggaatac
      721 gaggcggccc gcgagcgcca gggcttcatc gagggccgca tcatcgagct ggaggcgaag
      781 ctctccaact gccagatcat cgacccggcg ctgctcgacg acgacggccg cgtggtcttc
      841 ggctcgacgg tcgagctgat cgacacttcg ggcaaggaga cggtgagcta ccagatcgtc
      901 ggcgaggacg aggccgacat aaagctcggc aagatttcct actcctcccc gatcgcgaag
      961 gcgctcatcg ggaagagcga gggcgacgtc gtcgaggttc ggactccggg cgggctgaag
     1021 gagttcgaga tcggggacgt tcggtacgtc tagattcgga cgcggcctgc gcgacgcggg
     1081 acttgcgcag ggtgggcttc ccccggggcg cgggtttcgc gggccgcggc gtttcgcgga
     1141 cctcgcggat cctctccttc gggttttcgc ggtagagcac gagcaccttg ccgatcttct
     1201 gcaccggatg cgcacccagc ttctcgcaga tggccttcat ccactcctcg cgcgcgccgc
     1261 ggtcgtcggt cgccgcgcgc accttgatca attcgtgcgc cttcagcgag cggtcgatct
     1321 ccgcgacgac ctcgtcggtg aggcccttgt cgccgatgat gacgaccggg gagagggtgt
     1381 gggcttgcgc gcgcaactcg ctgcggcggg atgggttcag tggcatcggg cgagtataaa
     1441 ccgcggtttc ccgctggcgc tctcaagacg gtcacacgga gaacgcggag aacccacgga
     1501 gcgcagcgac cctagaggta ctttacgtcc ccgatctcga actccttcaa ccctcccgga
     1561 gtccgaacct cgacgacgtc gccctcgctc ttcccgatga gcgccttggc gatcggcgac
     1621 gagtaggaga tcttgccgag cttgatgtcg gcctcgtcct cgccgacgat ctggtagctc
     1681 accgtcgcgc cccccttggt gtcgatcagc tccaccgtcg aggcgaacac cacgcgcccg
     1741 tcgtcgtcga ggagcgccgg gtcgatgatc tggcagttgg agagcttcgc ctcgacttcg
     1801 atgatccggc cctcgatgaa cccctggcgc tcgcgcgccg cctcgtactc ggcgttctcg
     1861 gtgatgtcgc cgtgggccct cgcctcggcg atcgcggtga tcaccgcgtg gcgctccacc
     1921 gtcttcagct tgtgcagctc gttgcgcagc agctcggcgc caatcatcgt gatcgggatt
     1981 ttgctcatgt ggggcatgtg ggcaggctcg cgtgaagcga ttggatgtca taggggcgca
     2041 tttcctcgag gtgacgcatg ccgacgcagg ccgcgcgggc tcccgcgagg gtcgtgtagt
     2101 acgtgatccg gttctgcagc gccgaattgc ggatcgacca cgagtcctgg atggagcggc
     2161 ggtcttcctc gacggtgttc acgataaggc tcacttcccg gttcttcagc atgtcgacga
     2221 tgtgcggacg gccctcctgc accttgttca cgggctgtac cttgaggccc gccttctcga
     2281 ggctcgcggc ggttccaggc gtcgcgacga gcgcgaagcc caggtccgcc aggtcgcgcg
     2341 cgatctccac gaggcgctcc ttgtcctcgt cgcgcaccga caggaacacc ttgcccccgc
     2401 gcggaagctt cacgcccgcg gcgagctggc tcttcacgaa cgcctccgcg aatgtgtggc
     2461 ccacgcccat gacttcgccc gtcgacttca tttccggccc caggatcgtg tccactcccg
     2521 ggaacttgat gaagggaaag acggcctcct tgaccgaata gtacggcggg atcacttcct
     2581 tcgtcgcccc ctgtttcgcg aggctctgtc cgaccatgca gcgcgcggcg attttcgcga
     2641 gcggcaggcc ggtcgccttg ccgacgaaag gcacggtgcg cgaggcgcgc gggttgacct
     2701 cgagcacgta tatttcgtgc ccggcgccgg ccgtctgcgc attttgcgca ttctgtatcg
     2761 cgaactgcac gttcatcaga ccgacgac
//
LOCUS       DASVII010000364         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_448271,
            whole genome shotgun sequence.
ACCESSION   DASVII010000364 DASVII010000000
VERSION     DASVII010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448271"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1105)
                     /locus_tag="VF309_04785"
     CDS             complement(32..1105)
                     /locus_tag="VF309_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7465937.1"
                     /translation="MHELKAAQPSRHPSPTARASALEYLVFQRPDLDRAEDFLTGFGL
                     VVGSRTSDVLYMRGTLRSPYCYRIHRAPRARFVGAGFSVRTRNELERLARIHGASAIE
                     PCAHPGGGDRVSLRDPSGFTVEIVHGQQPAEERRAREPLAFNVGHERTRINSTQRPPV
                     EPPEVVRLGHYVLEVADFQATCGWYTHHLGFVPSDVQVLPDGSPAVVFMRLDLGDMPT
                     DHHTLAMVQGFMPEFNHAAFELVDADAVGMGQRVLREKGFTHAWGIGRHILGSQIFDY
                     WEDPWGNKHEHYCDGDLFTLDRPMGVYPVSREAMAQWGQPMPRAFTRPHFTPRAMLSA
                     MRNLRHSPDLTLDKVLTLARVFG"
     gene            1209..1847
                     /locus_tag="VF309_04790"
     CDS             1209..1847
                     /locus_tag="VF309_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7465938.1"
                     /translation="MTLKAQRKPDHRTRVGRERSARTETRILEAALGVFADMGPDAAK
                     IDDFVVAAGISRGTFYNHFQSVEELLEATSQWTTRELIETIEAALEGIEGPALRFGLG
                     LRLFFAKAQADPVWSRFVARVWKLGGIELPGRDLEQGFRLAVFRAPSAQAASDVLFGG
                     VREALLRIGTERTSAAYGAQMTEMCLQAIGTESRRMAAVLKHELPSFPAPLR"
BASE COUNT          324 a          604 c          643 g          317 t
ORIGIN      
        1 atgtgggtat ctccgagcaa gggaagagac atcagccgaa cacgcgggcg agagtgagca
       61 ccttgtccag ggtgaggtcg ggactgtggc gcaggttacg catggccgag agcatcgctc
      121 gcggcgtgaa gtgcggccgc gtgaacgcgc gcggcatcgg ctggccccat tgcgccatcg
      181 cttcacggct caccgggtac acgcccatcg gccgatcgag cgtgaacaaa tcgccgtcac
      241 agtagtgctc atgcttgttt ccccaggggt cttcccaata atcgaagatc tggctgccga
      301 ggatgtgacg tccgatgccc caggcgtgcg tgaagccctt ctcccgcaac acgcgctgcc
      361 ccatgccgac cgcgtcggcg tcgaccagct cgaacgccgc gtggttgaat tcgggcatga
      421 agccctggac catggccagc gtatggtggt cggtgggcat gtcgcccaga tcgaggcgca
      481 tgaacaccac ggcgggcgag ccgtcgggta ggacctgaac gtcgctcggt acgaagccaa
      541 ggtggtgcgt gtaccagccg catgtcgcct gaaagtccgc cacctcgagg acgtagtggc
      601 cgagccgcac gacctcgggc ggttccaccg gcgggcgctg tgtactgttg atgcgcgtgc
      661 gctcgtggcc gacgttgaac gccagcggtt ctcgcgcgcg ccgctcctct gcaggttgct
      721 ggccgtggac gatctcgacg gtgaaacccg acgggtccct caggctcacg cgatctccgc
      781 cgcccgggtg ggcgcagggt tcgatagcac ttgcaccgtg gatgcgcgcg aggcgttcga
      841 gctcgttgcg cgtgcgcacc gagaaccccg cgcccacgaa gcgcgcccgc ggcgcccgat
      901 ggatgcgata gcaatacggc gaccgcaagg tcccgcgcat gtacagcacg tcgctcgtcc
      961 ggctacccac cacgagtccg aagcccgtga gaaaatcttc cgcgcgatcg aggtccggac
     1021 gctggaagac gaggtattcg agcgccgacg cccgggcggt cgggctcggg tgccgcgagg
     1081 gttgtgccgc tttcagctcg tgcatcgagg atctccttca tgcccagctc gttggacacg
     1141 tatgaacatt atcatattga acgaagctgt caagttgaca aataggggca tggtggcatg
     1201 ctgctcccat gacgctcaaa gcgcagagga aaccggatca ccgcacccgc gtcggtcgcg
     1261 agcgcagcgc acgcacggag acgcgcatcc tcgaggccgc gctcggagta ttcgcggaca
     1321 tgggccccga cgcagcgaag atcgacgact tcgtcgtggc cgcgggcatc tcgcgcggca
     1381 cgttctacaa tcatttccag agcgtcgaag agctcctcga ggcgacctcc cagtggacga
     1441 cgcgcgagct catcgagacg atcgaagcgg cgctcgaggg catcgagggc ccggcgctcc
     1501 ggttcgggct cgggcttcgg ctgttcttcg cgaaggctca ggccgacccc gtctggtcgc
     1561 ggttcgtcgc gcgagtgtgg aagctcgggg gtatcgaact gccggggcgc gatctcgaac
     1621 aagggtttcg gctcgccgtc tttcgagcgc cgagcgccca ggccgccagc gatgtgctgt
     1681 tcggcggcgt gcgcgaagcg ctgctgcgca tcggaaccga gcgcacgtct gcagcgtacg
     1741 gcgcccaaat gacggagatg tgtcttcagg ccatcgggac cgagtcgcga cgaatggcgg
     1801 ccgttctgaa gcacgagctg ccgtcatttc ctgcgccgct gcggtagtgt cggctcgcgc
     1861 gaggcgcgac gcgattcgaa tcgcactc
//
LOCUS       DASVII010000365         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_53034, whole
            genome shotgun sequence.
ACCESSION   DASVII010000365 DASVII010000000
VERSION     DASVII010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53034"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF309_04795"
     CDS             complement(<1..186)
                     /locus_tag="VF309_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465939.1"
                     /translation="MAAIVLLEHRHQAQLGIHYMAYELARRWEAAGHRVHFHRGLGAA
                     PAGDLAILHHDLTVVPEA"
     gene            complement(187..600)
                     /locus_tag="VF309_04800"
     CDS             complement(187..600)
                     /locus_tag="VF309_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX7465940.1"
                     /translation="MGREIFDSFAGFDDFLVYLGVALVLLALFVAIYIRITPYREMEL
                     IRAGNMAASFSVSGSILGFIVPLASAVQHSVSLVDMVIWGVIALVVQVAAFVAVKLLI
                     PSITRDIPAGNGALGFFLGALSLGVGILSAACMSF"
     gene            complement(618..>1603)
                     /locus_tag="VF309_04805"
     CDS             complement(618..>1603)
                     /locus_tag="VF309_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX7465941.1"
                     /translation="EAATAELHARSIEAVARVVEKREFARFAIPEPFHALIERSWAGA
                     DKSLYGRFDLSWNGEGSPRMLEYNADTPTALLEASVVQWYWLQDVRKDADQFNSIHEK
                     LIERWKAIRGELPADGRVYFAGDGESVEDMGNLDYLRDTATQAGLDARAIDIADIGWD
                     GSRFVDLDARAMLALFKLYPWEWMVREAFGKNLVANAMKVIEPPWKMLLSNKAILPVL
                     WEMFPQHPNLLAASFEPGRFATDYVKKPLYSREGANVSINAGGEVVEAPGEYGAEGFI
                     WQEYKELARFGANYTVIGSWIVGEEPAGIGIREDDSPITRNSSRFVPHYFT"
BASE COUNT          274 a          538 c          542 g          249 t
ORIGIN      
        1 cgcctcgggc accaccgtga ggtcgtggtg caggatcgcc aggtcgccgg cgggagccgc
       61 gccgaggccg cgatggaagt gcacgcggtg cccggccgcc tcccagcgcc ttgccagctc
      121 gtaagccatg tagtggatgc cgagctgcgc ctggtggcga tgctcgagga ggacgatggc
      181 cgccactcag aagctcatgc acgccgccga gaggatcccc acgccgagcg agagcgcgcc
      241 gaggaagaac cccagcgcgc cgttgcccgc ggggatgtcg cgcgtgatcg acgggatcaa
      301 gagcttcacc gcgacgaagg cggcgacctg caccacgagc gcgatcacgc cccagatcac
      361 catgtccacc aggctcacgc tgtgctgcac ggcggaagcg agcggcacga tgaagccgag
      421 gatcgagccc gacacgctga acgacgccgc catgttgccc gcgcggatga gctccatctc
      481 gcggtagggc gtgatgcgga tgtagatcgc gacgaagagc gcgagcagca ccagcgcgac
      541 accgaggtag acgaggaaat cgtcgaaccc ggcgaacgaa tcgaagatct ctctacccat
      601 ggcgttgcct cctggagtca ggtgaagtag tgcgggacga aacggctgga attgcgcgtg
      661 atgggcgaat cgtcctcgcg gatgccgatg cccgcgggct cctcgcccac gatccacgag
      721 ccgatcacgg tgtagttggc gccgaagcgc gcgagctcct tgtattcctg ccagatgaag
      781 ccctcggcgc cgtattcgcc gggcgcctcc acgacttcgc cgcccgcgtt gatcgagacg
      841 ttggcgcctt cgcgcgagta gagcggcttc ttcacgtagt cggtcgcgaa gcgtcccggc
      901 tcgaagctcg ccgccagcag gttcggatgc tgcgggaaca tctcccacag gaccggcagt
      961 atcgccttgt tggacagcag catcttccac ggcggctcga tcaccttcat cgcgttggcg
     1021 acgaggttct tgccgaaggc ctcgcgcacc atccattccc acggatagag cttgaagagg
     1081 gcgagcatcg cgcgcgcgtc gaggtcgacg aagcgcgagc cgtcccagcc gatgtcggcg
     1141 atgtcgatgg cgcgcgcgtc gaggcccgcc tgggtcgcgg tgtcgcgcag gtaatcgagg
     1201 ttgcccatgt cctccaccga ctccccgtcg cccgcgaaat agactcgccc gtcggcgggc
     1261 agctcgccgc ggatcgcctt ccagcgctcg atcagcttct cgtggatgga attgaactgg
     1321 tccgcgtcct tgcgcacgtc ctgcagccag taccactgca ccacgctcgc ctcgaggagc
     1381 gcggtcggcg tgtcggcgtt gtactcgagc atccgggggc tgccctcgcc gttccaggat
     1441 aggtcgaagc gcccgtagag cgacttgtcg gcgccggccc aggagcgctc gatgagcgcg
     1501 tggaagggct cgggaatggc gaagcgcgcg aattcccgct tctcgacgac gcgtgccacg
     1561 gcctcgatcg agcgcgcgtg aagctccgcg gtcgccgcct cca
//
LOCUS       DASVII010000366         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_88582, whole
            genome shotgun sequence.
ACCESSION   DASVII010000366 DASVII010000000
VERSION     DASVII010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88582"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VF309_04810"
     CDS             <1..738
                     /locus_tag="VF309_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465942.1"
                     /translation="RGAIRLDGVSLSYPGGARPALSEVALDIRPGETVALVGPSGSGK
                     SSLIHLLARFYHPSAGRVTLDGHDLEELTLESLRRQIALVSQSVVLFNDTVAANIAYG
                     RAASVPESDIRRAAEAAHAIEFIDALPQGLATEIGENGAKLSGGQRQRIAIARAILKD
                     APILLLDEATSALDTESERAVQAALETLMRGRTTIVIAQRLATIEKADRIVVLAQGRI
                     AEIGAHRDLVAAGGVYAGLHRLQFAGA"
     gene            735..1832
                     /locus_tag="VF309_04815"
     CDS             735..1832
                     /locus_tag="VF309_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7465943.1"
                     /translation="MTLRILHTESSLGWGGQEIRVLTEARGVARAGHEVILAAPREAR
                     IFAEAPRFGVQVTAAAIARKGARGFLAMRHALAHGPFDVVNTHSSTDSWLAALACSPL
                     GKAPVLVRTRHISAPLPRNRATRWLYERSARIVTTGERLRAQVIAEAGVDASRVASIP
                     TGIDLDRYRHGDRLAARAALGLRADAKIVGIVATLRSWKGHSYLLKAIAAMRRPDVLL
                     VVVGDGPQRAALEQLAATLEIGEQVRFAGDQADVTPWLNAFDVFCLPSYANEGVPQAL
                     MQAMACELPVITTPVGSIDEIVEGEVTGIIVAPQNEVRLRVEISKLLDDAERRRELGA
                     AARERARARFGEALMVERMVAIFNDAVVHRG"
BASE COUNT          268 a          665 c          667 g          262 t
ORIGIN      
        1 cgaggcgcga tccgcctcga cggcgtctcg ctctcctatc ccgggggcgc tcgccccgcg
       61 ctgagcgaag tggcgctcga catccggccc ggcgagacgg tcgccctcgt gggcccttcg
      121 ggaagcggca agtcgagcct catccacctg ctggcgcgct tctaccatcc gagcgcgggg
      181 cgcgtcacgc tcgacggcca cgacctcgag gagctcacgc tcgagagcct gcgccggcag
      241 atcgccctcg tgtcgcagag cgtggtgctc ttcaacgaca cggtcgcggc gaacatcgcc
      301 tacgggcgcg ccgcgagcgt tccggagagc gatatccgcc gcgccgccga ggcggcgcac
      361 gcgatcgaat tcatcgacgc gctgccccag ggcctcgcga ccgagatcgg cgagaacggg
      421 gcgaagcttt cgggcgggca gcgccagcgc atcgcgatcg cgcgagcgat cctgaaggac
      481 gcgccgatcc tcctcctcga cgaagccacc tcggccctcg acaccgagag cgagcgcgcc
      541 gtgcaggccg cgctcgagac gctgatgcgc gggcgcacca ccatcgtgat agcgcaacgg
      601 ctggccacca tcgagaaggc cgaccgcatc gtggtgcttg cccagggacg catcgccgag
      661 atcggcgcgc accgcgatct cgtcgcggcg ggcggcgtgt acgcgggact ccatcgcctg
      721 caattcgcgg gcgcatgacg ttgcgcatcc tccacacgga gtcgtcgctc ggctggggcg
      781 gccaggagat ccgcgtgctc accgaagcgc gcggcgtggc gcgggcgggt cacgaggtga
      841 tcctcgccgc gccgcgcgag gcgcgcattt tcgccgaggc gccgcgattc ggagtgcagg
      901 tgaccgcggc cgcgatcgcg cgcaagggcg cgcgcggctt cctcgcgatg cggcacgcgc
      961 tcgcgcacgg accgttcgac gtcgtgaaca cccacagctc caccgacagc tggctcgcgg
     1021 cgctcgcgtg ctcgccgctc gggaaggcac cggtcctggt gcgcacgcgc cacatctccg
     1081 ctccgctgcc gcgcaatcgc gcgacgcgct ggctgtacga gcgctccgcg cgcatcgtga
     1141 cgaccggcga gcggcttcgc gctcaggtga tcgcggaggc gggcgtcgac gcctcacgcg
     1201 tggcgtcgat tccgacgggc atcgatctcg atcgctaccg gcacggggac cgcctcgcgg
     1261 cgcgcgcggc gctgggcctt cgcgccgacg cgaagatcgt cggaatcgtc gcgacgcttc
     1321 gcagctggaa gggccattcg tacctgctca aggcgatcgc ggcgatgcgc aggcccgacg
     1381 tgctcctcgt cgtcgtcggc gacggcccgc aacgcgcggc gctcgagcag ctggccgcga
     1441 cgctcgaaat cggggagcag gttcgattcg cgggtgacca ggccgacgtc acgccctggc
     1501 tgaacgcgtt cgacgttttc tgcctgccgt cctatgccaa cgaaggcgtg ccccaggcgc
     1561 tgatgcaggc gatggcgtgc gagctgccgg tgatcaccac gccggtcgga agcatcgacg
     1621 agatcgtcga gggagaagtc acgggcatca tcgtcgcgcc gcagaacgag gtgcggttgc
     1681 gtgtcgagat ctcgaagctc ctcgacgacg cggagcgccg ccgtgagctc ggcgcggccg
     1741 cgcgcgagcg cgccagggcc cgcttcggcg aggccctgat ggtcgagcgc atggtcgcga
     1801 tattcaacga cgcggtggtg catcgtgggt gagcgcttca cgacgcgctc gctcgcggtc
     1861 tc
//
LOCUS       DASVII010000367         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_88608, whole
            genome shotgun sequence.
ACCESSION   DASVII010000367 DASVII010000000
VERSION     DASVII010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88608"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VF309_04820"
     CDS             <1..1035
                     /locus_tag="VF309_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7465944.1"
                     /translation="PPTTAILLSTDMFTLIDEDAIVPFDDLAASADDKAWMKSFYPAF
                     MANSQTGGKTWGIPFQRSTIVLYWNKELFKEAGLDPTRPPATWKEMLEDAQKLTKRDS
                     SGNVTQYGVQIPSSGFPYWLFQGLTTPNGVELMNSAGTETYFDKPAVIEALAFWVDLG
                     RKHKVMPTGVGEWGTTPKDFFEKKIAMMWTTTGNLTNVRTNAKFDFGVAMLPAAKRRG
                     SPTGGGNFYVFKKAAKDQQLAAVKFAKWMTQPERAAQWGIDTGYVAVRPDAWKTAKMV
                     DYVKGFPVAAVARDQLEFSVAELSTHDNQRVTKALNDGIQAALTGAKTPEQAMKDSQA
                     EAARVLRPYK"
     gene            1073..>1827
                     /locus_tag="VF309_04825"
     CDS             1073..>1827
                     /locus_tag="VF309_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465945.1"
                     /translation="MTLACAAHADADAKPKVYALISAVGSEISYVRQRLQVGSNFEPY
                     YRNTLPVPDSTVDTAVLRGLERAILQEDPDAKRVYLRLAPTEVRDVLPYKRGDVVPER
                     ALAALARMPDRKDWDRIILVTPRFLGAAREGMGTKLHGIGVYVQPLGNGRRGNLDLEV
                     GATVDPDTVSPEGEKSRSNVYIAPYFYAQVWIIDAKTMQVLETRERYDFQRLYDPNSA
                     AIDVAKSIPVEQLAAMVQKFIERASARALNESIG"
BASE COUNT          345 a          613 c          598 g          271 t
ORIGIN      
        1 ccgccgacca ccgcgatcct gctgtccacc gacatgttca cgctgatcga cgaggacgcg
       61 atcgtgccgt tcgacgatct cgccgcgagc gcggacgaca aggcgtggat gaagagcttc
      121 tatcccgcct tcatggccaa cagccagacg ggcggcaaga cctgggggat ccccttccag
      181 cgctcgacga tcgtcctcta ctggaacaag gagctcttca aggaagcggg cctcgacccg
      241 acccgcccgc cggcgacctg gaaggaaatg ctcgaggacg cgcagaagct caccaagcgc
      301 gattcctcgg gcaacgtcac gcagtacggc gtccagatcc catcgtcggg atttccgtac
      361 tggctcttcc agggcctcac gacgcccaac ggcgtggagc tcatgaattc cgcgggcacc
      421 gagacctact tcgacaagcc cgcggtgatc gaggccctcg ccttctgggt ggacctcggc
      481 cgcaagcaca aggtgatgcc cacgggcgtg ggcgagtggg gcacgacgcc gaaggatttc
      541 ttcgagaaga agatcgcgat gatgtggacc accacgggca acctcaccaa cgtgaggacg
      601 aacgccaagt tcgacttcgg cgtcgcgatg ctgccggccg ccaagcgccg cggctctccc
      661 acgggcgggg gcaacttcta cgtcttcaag aaggctgcga aggaccaaca gctcgcggcg
      721 gtgaaattcg cgaaatggat gacgcagccc gagcgcgccg cgcagtgggg catcgacacg
      781 ggctacgtcg cggtgcgtcc cgacgcgtgg aagaccgcga agatggtgga ctacgtgaag
      841 ggcttccccg tggccgccgt tgcccgggac cagctggagt tctcggtcgc ggagctttcc
      901 acccacgaca accagcgagt caccaaggcg ctgaacgacg gcatccaggc ggcgctcacc
      961 ggtgcgaaga cgccggagca ggcgatgaag gactcgcaag cggaagcggc ccgcgtcctg
     1021 cgcccgtaca agtgacatga acgccatccg atggatactg ctggcttgct ccgtgacgtt
     1081 ggcgtgcgcc gcgcacgccg atgccgatgc gaagccgaag gtctatgcgc tgatctccgc
     1141 ggtgggctcg gagatcagct acgtgcgcca gcgcctgcag gtgggctcga acttcgagcc
     1201 gtactaccgc aatacgttgc ccgtgccgga ttcgaccgtc gacacggccg tgctgcgcgg
     1261 cctggagcgc gcgatcctgc aggaggatcc cgacgccaag cgggtctacc tgcgcctcgc
     1321 gcccaccgag gttcgcgacg tactgcccta caagcgcggc gacgtggtgc ccgagcgcgc
     1381 gctggcggcg ctcgcgcgca tgccggatcg caaggactgg gaccgcatca tcctcgtcac
     1441 gccgcgcttc ctcggcgccg cgcgcgaagg catgggcacc aagctgcacg gcatcggcgt
     1501 ctacgtgcag ccgctcggga acggccggcg cggcaacctc gacctagagg tgggcgccac
     1561 ggtcgacccg gacacggtct cgcccgaggg cgagaaatcg cgttccaacg tgtacatcgc
     1621 accgtatttc tacgcgcagg tgtggatcat cgacgcgaag acgatgcagg tgctggaaac
     1681 ccgcgagcgc tacgacttcc agcgcctcta cgatcccaac tcggctgcca tcgacgtggc
     1741 gaagtcgatc ccggtggagc aactcgccgc gatggtgcaa aagttcatcg agcgcgccag
     1801 cgcacgcgcg ttgaacgagt ccatcgg
//
LOCUS       DASVII010000368         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_125305,
            whole genome shotgun sequence.
ACCESSION   DASVII010000368 DASVII010000000
VERSION     DASVII010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125305"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VF309_04830"
     CDS             <1..824
                     /locus_tag="VF309_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7465946.1"
                     /translation="HLGGDTIRKRLPSVLEIGHNFANVDITREAIPVVPTIHYQMGGI
                     PTNYHGEVVTPAAGNPSTPVPGFYAAGECGCASIHGANRLGTNSLTDLLVIGKSSAMS
                     MIEYMKGPGKAHRDLPAGAGELSRARLARLDSQTGGEEVGDVRSEMQRIMQAHCGVFR
                     FPELLAQGVEKIKAVAERAKRTQIKDKSKVFNTARLEALELDNLIEVARATMICAQAR
                     QESRGAHDRADFHKRDDVNWLKHSLWFREGDRLDYKPVHLKPLTVESFEPKARVY"
     gene            826..1521
                     /locus_tag="VF309_04835"
     CDS             826..1521
                     /locus_tag="VF309_04835"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7465947.1"
                     /translation="MKFRIQRYDPDKDAKPYMQDYDVEMRPSDRMLLDALVRIKEHDD
                     TLALRRSCREGVCGSDAMNINGKNGLACITKLADIVQPVVIKPLPGLPIIRDLIVDMT
                     QFFNQYHSVKPYLVNAEPAPEKERLQSPREREQLDGLYECILCACCSTACPSFWWNPD
                     KFVGPAGLLNAYRFIADSRDTATNERLDDLEDPYRLFRCHTIMNCVDVCPKGLNPTLA
                     IGRIKELLVKRAV"
     gene            1548..1796
                     /locus_tag="VF309_04840"
     CDS             1548..1796
                     /locus_tag="VF309_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX7465948.1"
                     /translation="MDDRVIERLKWKSRRGLLELDIVFDRFWKGAGATMDEADVAALE
                     RLLALPDNDLLDLVMGRAEMPEARLRPVLDKLRAAEAP"
     gene            1810..>2744
                     /gene="gltA"
                     /locus_tag="VF309_04845"
     CDS             1810..>2744
                     /gene="gltA"
                     /locus_tag="VF309_04845"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7465949.1"
                     /translation="MDAKGKVTLNYGPDKNLDLPTYGGSIGPDVLDIRALYGKTGMFT
                     YDPGFMSTAACQSKITYIDGDKGVLLYRGYPIEQLAVHCDFLEVCYLLLNGELPKKAE
                     KEKFDWTVTRHTMVHEQLARLFQGFRRDSHPMAVMVGVVGALSAFYHDSLDINNPEHR
                     EISAFRLIAKLPTIVAMAYKYNMGQPFIYPKNNLGYTANFMRMMFGVPAEDYVVNDVL
                     VRALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALQ
                     MLMDIERDGGVAKVGEFIKRAKDKNSGVKLMGFGHR"
BASE COUNT          529 a          909 c          872 g          434 t
ORIGIN      
        1 ctcacctggg cggcgacacg atcaggaagc gcctgccctc ggtgctcgag atcggccaca
       61 acttcgccaa cgtcgacatc acgcgcgagg cgatcccggt cgtgccgacg atccattacc
      121 agatgggcgg catcccgacg aattaccacg gcgaggtcgt cacgccggct gcgggaaatc
      181 ccagcactcc ggtccccggc ttctacgcgg cgggcgagtg cggctgcgcg tcgatccacg
      241 gcgcgaaccg cctcggcacc aactcgctca cggacctgct cgtgatcggc aagagctcgg
      301 cgatgtcgat gatcgagtac atgaagggcc cggggaaggc gcaccgcgac cttccggccg
      361 gcgcgggcga gctttcccgc gcgcggctcg cgcgcctcga ttcgcagacg ggcggcgagg
      421 aagtgggtga tgtccgctcc gagatgcagc gcatcatgca ggcccactgc ggcgtgttcc
      481 gctttccgga gctcctcgcg cagggcgtgg agaagatcaa ggcggtcgcc gagcgcgcga
      541 agcgcacgca gatcaaggac aagtcgaagg tgttcaacac cgcgcgcctc gaggcgctcg
      601 agctcgacaa cctcatcgag gtggcgcgcg cgacgatgat ctgcgcgcag gcgcgccagg
      661 agagccgcgg cgcccacgac cgcgccgact tccacaagcg cgacgacgtg aactggctca
      721 agcactcgct gtggttccgc gagggcgacc gcctcgacta caagcccgtg cacctgaagc
      781 cccttaccgt cgaatccttc gaaccgaaag cccgggtcta ctgacatgaa attccgcata
      841 cagcgctacg atcccgacaa ggacgcgaag ccctacatgc aggactacga cgtcgagatg
      901 cgtccttcgg accgcatgct tctcgacgcc ctggtgcgca tcaaggagca cgacgacact
      961 ctcgcgctgc gccgctcctg ccgcgagggt gtgtgcgggt cggacgcgat gaacatcaac
     1021 ggcaagaacg gcctcgcatg catcaccaag ctcgccgaca tcgtgcagcc ggtggtgatc
     1081 aagccgctgc cggggctgcc catcatccgc gacctcatcg tggacatgac gcagttcttc
     1141 aaccagtacc attcggtgaa gccgtacctg gtgaacgccg agcccgcgcc cgagaaggag
     1201 cgcctgcagt cgccgcgcga gcgcgagcag ctggacgggc tctacgaatg catcctgtgc
     1261 gcgtgctgct cgacggcgtg cccgagcttc tggtggaacc ccgacaagtt cgtcggcccc
     1321 gcggggctgc tgaacgccta ccgcttcatc gccgacagcc gcgacaccgc gaccaacgag
     1381 cgcctcgacg atctcgagga tccgtatcgc ctgttccgct gccacaccat catgaattgc
     1441 gtcgacgtct gcccgaaggg cctcaatccg acgctcgcga tcggacgcat caaggagttg
     1501 ctcgtcaagc gcgcggtatg aacgacaaga gaccgattca cgcttgaatg gacgacagag
     1561 tcatcgagag attgaagtgg aagagcaggc gcggattgct ggagctggat atcgtcttcg
     1621 acaggttctg gaagggtgcc ggagccacaa tggatgaagc tgacgtggcg gcgctcgaac
     1681 gattgctcgc gctccccgac aacgatctgc tcgacctcgt gatgggacgg gccgagatgc
     1741 cggaggcacg cctccggcct gtgctggata aactgcgcgc cgccgaggcg ccataggagc
     1801 caccgaacga tggacgccaa aggcaaggtc acactcaact acggtcccga caagaatctc
     1861 gacctgccga cctatggcgg atcgatcggc ccggacgtgc tcgacatccg cgcgctctac
     1921 ggcaagacgg ggatgttcac ctacgacccg gggttcatgt ccacggcggc gtgccagtcg
     1981 aagatcacct acatcgacgg cgacaaggga gtcctcctgt atcgcggcta tccgatcgag
     2041 cagctcgcgg tgcactgcga cttcctcgag gtctgctacc tgctgctgaa cggcgagctg
     2101 ccgaagaagg ccgagaagga gaaattcgac tggaccgtga cgcgccacac gatggtgcat
     2161 gagcagctcg cgcgcctctt ccagggcttt cgccgcgact cgcacccgat ggcggtgatg
     2221 gtcggggtcg tgggcgcgct gtcggccttc taccacgact cgctcgacat caacaatccc
     2281 gagcatcgcg agatttcggc gtttcgcctg atcgcgaagc tcccgaccat cgtcgccatg
     2341 gcctacaagt acaacatggg gcagcccttc atctatccga agaacaacct cggctacacg
     2401 gcgaacttca tgcgcatgat gttcggcgtg cccgccgagg attacgtggt gaacgacgtc
     2461 ctggtgcgcg cgctcgaccg catcttcatc ctgcacgccg accacgagca gaacgcgtcg
     2521 acctccaccg tgcggctcgc gggttcctcc ggcgcgaatc cgttcgcgtg catcgccgcg
     2581 ggaatcgcgt gcctctgggg cccggcgcac ggcggcgcca acgaggcggc gctgcagatg
     2641 ctcatggaca tcgagcgcga cggcggcgtc gcgaaagtgg gcgagttcat caagagggcg
     2701 aaggacaaga attccggcgt gaagctgatg ggtttcggcc accg
//
LOCUS       DASVII010000369         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_377144,
            whole genome shotgun sequence.
ACCESSION   DASVII010000369 DASVII010000000
VERSION     DASVII010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377144"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1362)
                     /locus_tag="VF309_04850"
     CDS             complement(187..1362)
                     /locus_tag="VF309_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7465950.1"
                     /translation="MRPAFLTVYLKELKDALRDRRTAIMILVASIITGPVTLVLVAQF
                     ISGLEEKSATLKVRIVGQQHAPQLINFLQRNDVEIETAPADYEARVREGRLDAVIVIP
                     EDFDERLLAGDNAKVEIVYDDSRADSGPAIRQAERLMRAFNHETGMLRMMARGVSPDL
                     VEPVKVERVNTATPRQKGAFILFLIPMFAILSPLLGGMTVAIDSTAGERERGSLEPLL
                     ANPVPTGQIVTGKWLAAWTLATTVAVLTLTGFVIAALLHSQKKLATLMTFGTPELFQF
                     ILIVIPFAAMTSALQMLICTYGRSYREAQTYVSYLATVASFVPVIVMFSGLKDALWQL
                     AIPVLGQQMVLSRVVRGDALAPADYLLPSAVALALAAICVALVARLLREERIIFGRS"
     gene            complement(1359..>1556)
                     /locus_tag="VF309_04855"
     CDS             complement(1359..>1556)
                     /locus_tag="VF309_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465951.1"
                     /translation="LRDAGKCVLFSSHVMQEVSALCDAIVIVARGAVVAHGTKESLLA
                     QSGCTNLEDAFVRLSGEEAPA"
BASE COUNT          276 a          506 c          542 g          232 t
ORIGIN      
        1 cgcgatgagg acgccgcctg cctcgatggc ctccagcacc gcctgcgcga actcgacctg
       61 ctgctcgcga tggcgaaagc ctgcgaccgc tttttcgagc ggaccgccgg aggcgaagag
      121 gggagcgaca tccatgggag atccagttcc ggtaacgggc aacggggctg tctcgccgac
      181 ttcgcgctag gagcgcccga agatgatgcg ctcctcgcgc agcagccgcg cgacgagggc
      241 cacgcagatc gcggcgaggg ctaacgcgac cgcggagggc aggagatagt cggccggcgc
      301 gagcgcgtcg ccgcgcacca cgcgcgagag caccatctgc tggccgagca ccgggatggc
      361 cagctgccat agcgcgtcct tcagcccgga gaacatcacg atcaccggca cgaaagaggc
      421 gaccgtcgcg aggtagctca cgtaggtctg cgcctcgcgg tacgagcgtc cataggtgca
      481 gatgagcatc tgcagggcgg aggtcatcgc cgcgaaggga atcacgatca ggatgaactg
      541 gaaaagctcc ggcgtgccga acgtcatcag cgtcgcgagc ttcttctgcg agtgcagcag
      601 cgccgcgatg acgaaaccgg tcagggtgag caccgcgacg gtcgtggcga gggtccaggc
      661 cgcgagccat ttgcccgtga cgatctggcc cgtggggacg gggttggcga gcagcggctc
      721 gagcgagccg cgctcgcgct cgcccgccgt ggagtcgatc gccaccgtca tgccgcccag
      781 cagcggcgag aggatcgcga acatgggaat gagaaagagg atgaaggcgc ccttctggcg
      841 cggcgtggcg gtattcacgc gctcgacctt caccggctcg accagatccg ggctgactcc
      901 gcgcgccatc atgcgaagca ttcccgtctc gtggttgaac gcccgcatga gacgctcggc
      961 ctggcggatg gcgggccccg aatcggcgcg cgagtcgtcg tagacgatct cgaccttcgc
     1021 gttgtcgccc gcgagaaggc gctcgtcgaa gtcctcggga atcacgatca cggcgtcgag
     1081 gcggccttcg cggacgcgcg cctcgtagtc cgcgggggcg gtctcgattt ccacgtcgtt
     1141 gcgttgcagg aagttgatga gctgcggggc atgctgctgg ccgacgatgc gcaccttgag
     1201 cgtcgcggac ttctcctcca gcccggagat gaactgcgcg acgaggacca gcgtgacggg
     1261 cccggtgatg atcgaggcca cgaggatcat gatcgcggtg cgccggtcgc gaagcgcatc
     1321 cttcagctcc ttcaggtaca ccgtgaggaa tgccggcctc atgcgggcgc ctcctcgccc
     1381 gaaaggcgga cgaacgcatc ctcgaggttc gtgcacccgc tttgcgcgag caggctctcc
     1441 ttggtcccgt gagcgaccac ggctccccgc gcgacgatca cgatcgcgtc acaaagcgcc
     1501 gagacctcct gcatcacatg gctcgagaag aggacgcact tgccggcgtc gcgaag
//
LOCUS       DASVII010000370         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_341038,
            whole genome shotgun sequence.
ACCESSION   DASVII010000370 DASVII010000000
VERSION     DASVII010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341038"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VF309_04860"
     CDS             <1..1146
                     /locus_tag="VF309_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7465952.1"
                     /translation="YALEVEVGVDAEAHGDAPGALAAFVRAAVIDPSGIEALDGIRRV
                     ALAGGNPRGAAQAGMRLGTVLRTPRRAAVELRRAAVLWRQLGMPIEAKVAYFHALARE
                     PESEEVFTALRGLLRDDGDDKDLEHLLTLRLAVVSTPEARLPLLIERGLLRHDRLGKQ
                     DAAIDDFKRILKIDPAERRALRELATLAAERQCHPQAIAYLERLLAIEDAASARAALL
                     LELAEAHESAGDPSRALDILHDVIARTPSDLSARQRLVDARSRTHPSVWRFGALERQA
                     GNHRATVRRDEQTAPAHHGPRLGRARIDEVLVEARVLRERSMVLGIRRRHQEHVIGDR
                     SASQIRIRGRERRHAPASRSDRNHRDENDELPHVERTRARSRRLRYC"
     gene            complement(1137..>1536)
                     /locus_tag="VF309_04865"
     CDS             complement(1137..>1536)
                     /locus_tag="VF309_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465953.1"
                     /translation="RVLATIQKYMQGLAGIDRAANAFVIRGTGLVAAAKDPDEQESSR
                     WQFLARRALATKDPNSSHGEIVDPDAYAMSFWYDAALVVLLAEPYALDFVRHRCRQVS
                     RELCNLLPMLDPDPEAPAAIGPRPRRPTQQ"
BASE COUNT          242 a          508 c          557 g          229 t
ORIGIN      
        1 tacgcgctcg aggtcgaggt cggcgtcgac gccgaggcgc acggcgatgc cccgggtgcg
       61 ctcgccgcgt tcgtgcgcgc ggcggtgatc gatccgagcg gcatcgaggc cctcgacggg
      121 attcgacgtg tggccctcgc cggcgggaac ccgcgcgggg ccgcccaggc cgggatgcgc
      181 ctcggaacag tgctccgcac gccccggcgc gccgctgtcg agctgcggcg cgccgccgtc
      241 ctctggcgcc agctgggcat gcccatcgag gcgaaggtgg cctatttcca cgccctcgcg
      301 cgcgagccgg aatccgagga ggtgttcacg gcgctgcgcg ggctgttgcg cgacgacggt
      361 gacgacaagg atctcgaaca tctgctcacg ctgcggctgg cggtggtgag cacgcccgag
      421 gcgaggcttc cgctgctgat cgagcgggga ttgctccgcc acgatcgcct gggaaaacag
      481 gatgctgcga tcgatgactt caagcgaatc ttgaagattg atcccgccga acggcgggcg
      541 ctccgtgagc tcgccacgct ggccgccgaa cgccagtgtc atcctcaggc gatcgcctac
      601 ctcgagcgct tgctggccat cgaggacgcc gcctccgcgc gcgccgctct gctgctcgag
      661 ctggcggagg cccacgagtc ggcgggcgat ccgtcgcggg cgctcgacat cctgcacgac
      721 gtgatcgccc ggacgccctc cgacctgagc gcccgccagc ggctggtcga cgcccggtcg
      781 cggacacatc cttccgtttg gcgctttggc gcgctagaac gacaggcagg gaaccatcgc
      841 gcgaccgttc ggcgcgacga gcagacagcg ccggcgcatc acggaccacg tctcggtcgt
      901 gcacgcatcg acgaggttct cgtggaggct cgcgtactgc gcgaacggtc gatggtcctt
      961 gggatccggc ggcgacacca agagcatgtg atcggcgacc gcagcgcatc gcagatccgc
     1021 attcgcgggc gcgagcggcg gcacgcgccc gccagccgta gcgaccgcaa ccatagagac
     1081 gagaacgacg agcttccaca tgtcgagcgg acacgcgcgc gatcgcgacg gttgcgctac
     1141 tgctgagtag gccgacgcgg acgcggaccg atcgcggcgg gcgcttcggg atccggatcg
     1201 agcatgggca gcagattgca caattcgcgc gagacctggc ggcaacgatg gcgaacgaag
     1261 tcgagcgcgt agggctcggc gaggagcacg accagtgcgg cgtcgtacca gaagctcatc
     1321 gcatacgcgt cggggtcgac aatctcgccg tgcgagctgt tgggatcttt ggtggcgagc
     1381 gcacggcgcg cgaggaactg ccagcgcgag gattcttgtt cgtcggggtc tttggcagcg
     1441 gcgacgaggc cggtgccacg gatcacgaat gcgttggcgg cgcgatcgat gccggcgagg
     1501 ccctgcatgt atttttggat cgtggccagc acgcgg
//
LOCUS       DASVII010000371         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_304840,
            whole genome shotgun sequence.
ACCESSION   DASVII010000371 DASVII010000000
VERSION     DASVII010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304840"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1075)
                     /locus_tag="VF309_04870"
     CDS             complement(194..1075)
                     /locus_tag="VF309_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7465954.1"
                     /translation="MDATPHFMDILTMTNRGEAFYFRPLDQPPSGRACVLSLGIREVM
                     PPGFIRHVGLYADWGYLFMDFHGPALVDPTERPDMVERGFILWRPGVVRHYGNPDAPW
                     EHSWLNATGSAIDEAVAEHGVLLDRVMHLDVGAISLKYLHALYDEVQEHVVADDQIVE
                     GLIGIWIREIARAAGTGGVRPPARVTEARRFIEAHYAEPLELEALARRAALSVSQFSM
                     VFRAHFGVPPMQYAYRLRLRKAAQLLGDSTLAIGEVAARVGYEDPLHFSRQFRKYMGQ
                     NPRLYRASRGPKTVRKR"
     gene            1164..>1735
                     /locus_tag="VF309_04875"
     CDS             1164..>1735
                     /locus_tag="VF309_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HEX7465955.1"
                     /translation="MFARDASKPTLSRCLWVLLVGFVLAGCKGDIGDVDNVDGVDDVG
                     RATPPGGLPVDGTLGIGATGIRRMTVWEYDNTLRDVLGDTSRPGAALLPEDVRAPFDN
                     DFIAQETSKVLVEAAETLSANVAARLIADVPRRASVTGCQPSGPADTACLGAFIERLG
                     RRAFRRPPRPEESAALLDLARRFAAPPGDV"
BASE COUNT          319 a          554 c          570 g          292 t
ORIGIN      
        1 ggagatgtaa tgagcgcctc acggtaccgg ttgggggctt ggcgccagat gcgggttccc
       61 ttgggactcc tgtgaggcgc cggccgtagc ggaggtgccg ccatccaagg cggcgccgcg
      121 aagacgagga aggcacctcc ggcggccgat ccgtcagagc gcacgtcgca gctcggtcaa
      181 tggtgtggag gactcaccgc tttcgcaccg tcttcggacc gcgcgacgcc cggtaaagtc
      241 gcggattttg ccccatgtat ttacgaaact gtcgcgagaa gtggagcgga tcctcgtatc
      301 ccacgcgtgc ggcgacttcg ccgatagcga gcgtgctatc gccgagcagc tgagcagcct
      361 tccgcaagcg aaggcgatac gcgtactgca tgggcggcac gccgaagtgc gccctgaaca
      421 ccatgctgaa ttgcgaaaca gaaagcgccg cacgccgcgc gagagcctcg agctcgagcg
      481 gctctgcgta gtgcgcctcg atgaagcgac gcgcttcggt gacacgtgcg ggaggcctca
      541 ccccgccggt gccggccgct cgcgcgattt cgcggatcca gattccaatg agaccctcga
      601 cgatctggtc atcggcaacg acatgctcct gcacctcgtc gtaaagggcg tgtaggtact
      661 tgagagaaat cgcgcccacg tcgaggtgca tcacccgatc gagcagaacg ccatgttcgg
      721 caacggcttc gtcaatcgcg gatcccgtcg cgttcaacca ggagtgctcc catggcgcgt
      781 cgggattgcc atagtggcgc acgacgccgg gccgccagag aatgaatccg cgctcgacca
      841 tatcggggcg ctccgtcggg tccaccaggg ccggcccgtg aaagtccatg aacaggtagc
      901 cccagtcggc gtatagcccg acgtgacgaa tgaacccagg gggcatgacc tcgcggatcc
      961 cgagcgagag cacgcaggct cgtccgctgg gcggctggtc caatgggcgg aagtagaacg
     1021 cttcgccgcg attggtcatc gtaagaatat ccatgaaatg cggagttgcg tccataccct
     1081 tctcgagtgg atcgagatta aatcatgggt acaagatatc gcttggcgcg cgagccgcgc
     1141 acttacaccc gagaagaaca tccatgttcg caagagacgc gtccaaaccg acactctccc
     1201 gctgcctgtg ggtgctgctg gttggctttg tgctggccgg gtgcaaaggt gacatcggcg
     1261 acgtcgataa cgtcgatggc gtcgacgacg tcggtagggc cacgccgccg ggcggtctcc
     1321 cggtcgatgg cacgctcggc atcggcgcca ccggcattcg gcgaatgacg gtttgggagt
     1381 acgacaacac tttgcgggac gtgctgggag atacgagccg cccgggcgcg gccctattgc
     1441 cggaagacgt tcgcgcccct ttcgacaacg acttcatcgc gcaagaaacc tcgaaggtgc
     1501 tggtggaagc agccgagacc ctctcggcca acgtggcggc acgcctgatc gccgacgttc
     1561 cacggcgcgc atcggtgacc ggttgccagc ccagcggacc agccgacacc gcgtgcttgg
     1621 gcgccttcat cgagcggttg ggccggcgcg ccttccggcg accgcctcgc cccgaagaat
     1681 ccgccgcgct gctcgatctc gcccggcgct tcgctgcgcc gccgggtgat gtctg
//
LOCUS       DASVII010000372         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_377235,
            whole genome shotgun sequence.
ACCESSION   DASVII010000372 DASVII010000000
VERSION     DASVII010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377235"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VF309_04880"
     CDS             <1..625
                     /locus_tag="VF309_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7465956.1"
                     /translation="FDLGSKGVNERNVYVLLRTGQDLLDLPGGATALDVTVFDLWSAE
                     VIAQRITARVGLEADSWIRTNSQLFMALNAQTISNTVLRLAVGISVALGIASVLVVSV
                     VQKSREIGILRAMGASRRQVMNVFLIQGGIVGLVGSFIGSGLAAMFLFAWQSTLKNPD
                     GTPLFLLQFDPKLVAWAAAIATVTGLLAAMTPARRAAKLDPVVAIRA"
     gene            618..1352
                     /locus_tag="VF309_04885"
     CDS             618..1352
                     /locus_tag="VF309_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7465957.1"
                     /translation="MPDEVLRLEGVRKSYRSDGAAPNEVLHGIDLSLGRGEFVALMGP
                     SGSGKSTLLNVIGLLDRPDGGVLRIQGKDTSRLDEAALTKLRGHSIGFVFQYHYLIPA
                     FTAIENVMMPMLLEKGRPDVAMREKAAALLEKVGLTPWLDSRVNNLSGGQQQRVAVAR
                     ALAMDPPLVLADEPTGNLDTKSADGVFGLMREVNAASGTAFLFVTHNPALAERCDRTI
                     QLVDGRIVGDAPDRVPISEKAPALAR"
     gene            complement(1321..>1520)
                     /locus_tag="VF309_04890"
     CDS             complement(1321..>1520)
                     /locus_tag="VF309_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465958.1"
                     /translation="SEDAFDAALARMLAPRAIAARDFSGSIPEARFYFDTDHLNREGL
                     SRFVEHDLKAVLTEPAPAPSR"
BASE COUNT          231 a          500 c          537 g          252 t
ORIGIN      
        1 cttcgacctg ggctccaagg gcgtgaacga gcgcaatgtc tacgtgctgc tgcgcaccgg
       61 gcaggacctc ctcgatcttc cgggtggcgc cacggccctc gacgtgacgg tgttcgatct
      121 ctggagcgcg gaggtcatcg cgcagcgcat caccgcccgc gtggggctcg aggccgacag
      181 ctggattcgc accaacagcc agctcttcat ggcgttgaac gcgcagacga tctccaacac
      241 ggttctgcgg ctcgccgtcg ggatctcggt cgcgctcggc atcgcgagcg tgctggtggt
      301 ctcggtcgtg cagaagtcgc gcgagatcgg gatcctgcgc gccatgggcg cctcgcgccg
      361 ccaggtgatg aacgtgttcc tgatccaggg cgggatcgtc gggctggtgg gctcgttcat
      421 cggctcgggg ctcgccgcga tgttcctctt cgcctggcaa tcgacgctca agaatcccga
      481 cggcacgccg ctcttcctgc tgcagttcga tccgaagctg gtggcatggg ccgcggcgat
      541 cgcgaccgtc acgggtctcc tcgccgcgat gacgccggcg cgccgcgcgg cgaagctcga
      601 cccggtggtg gcgatccgtg cctgacgagg tcctgcgcct cgagggcgtg cgcaagtcgt
      661 atcgcagcga cggcgccgcg ccgaacgagg ttctccacgg catcgacctg tcgctcggcc
      721 gcggcgagtt cgtcgcgctc atgggcccgt cggggtccgg gaagagcacg ctcctcaacg
      781 tgatcggact tctcgaccgc cccgacggcg gcgtgctgcg catccaggga aaggacacgt
      841 cgcgcctcga cgaggcggcg ctcacgaagc tgcgcggtca cagcatcggc ttcgtgttcc
      901 agtaccacta tttgattccg gccttcaccg cgatcgagaa cgtgatgatg ccgatgctgc
      961 tcgagaaggg aaggcccgac gtggcgatgc gcgagaaggc cgccgcgctc ctcgaaaagg
     1021 tgggcctcac gccatggctc gacagccgcg tgaacaacct ttccggcggg caacagcagc
     1081 gcgtcgcggt cgcgagggcg cttgcgatgg acccgccgct ggtcctggcc gacgagccca
     1141 cgggcaacct cgacacgaaa tcggccgacg gggtcttcgg tctcatgcgc gaagtgaatg
     1201 ccgcgagcgg cacggcgttt ctgttcgtga cccacaaccc cgcgctcgcg gagcgttgcg
     1261 atcgcacgat ccagctcgtg gatggacgca tcgtggggga tgcgccggac cgcgtgccga
     1321 tcagcgagaa ggcgccggcg ctggctcggt aaggacggct ttcaggtcgt gctcgacgaa
     1381 tcgcgacagc ccctcgcggt tgaggtggtc ggtgtcgaag tagaagcgcg cctcggggat
     1441 cgagcccgag aagtcgcgcg ctgcgattgc gcgcggggcg agcattcgcg cgagggcggc
     1501 atcgaatgcg tcctcggacg
//
LOCUS       DASVII010000373         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_664548,
            whole genome shotgun sequence.
ACCESSION   DASVII010000373 DASVII010000000
VERSION     DASVII010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664548"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1649
                     /locus_tag="VF309_04895"
     CDS             <1..>1649
                     /locus_tag="VF309_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7465959.1"
                     /translation="LALLANPGTTIDELIRIVPAPDFPTAGIIYGVGDLQEGYRTGRG
                     RVIMRARTHVEDIGKGERQAIIIDELPYQVNKAQLLVKIGELVREKRIEGISDLRDES
                     DKSGMRAVIELKRGEVAEIVLNNLYKLTPMQESFGMNMVALLDGQPKLLNLKQFLDAF
                     LRHRREVVTRRTVFDLRKARDRGHILEGLAVALSNVDEVIAIIKGAATPAVAKTGLMA
                     RAWRSPLVEQMLARATAEQYRPEALPKEFGLVKDSPDAAGYRLSETQAQRILEMQLQR
                     LTGLEQDKILSEYREVIATVEDLLDILAKPERVTRIIGDELAEVKREFGDKRRSEIVV
                     NGEELSLEDLITPEDVVVTLSHAGYVKSQPLDEYRAQKRGGRGKQATTTKEDDFVEKL
                     FIANTHDHVLCFSNKGKAYWLKVYEVPQGSRTSRGKPIVNLLKMDEDERINAILPVKE
                     FREDCYVFFATQEGVVKKTSLSDYSRPRPSGIIAVDLDEGDRLIGVALTDGKHDVMLF
                     SDEGKAVRFDENDVRPMGRGARGVRGMNIAKGGKVIALLVA"
BASE COUNT          313 a          548 c          547 g          241 t
ORIGIN      
        1 tctcgccctg ctggccaatc ccgggacgac catcgacgag ctgatcagga tcgtccccgc
       61 gccggacttc cccacggcgg gaatcatcta cggggtgggc gacctgcagg agggctatcg
      121 caccgggcgc gggcgcgtga tcatgcgcgc gcgcacccac gtcgaggaca tcggcaaggg
      181 cgagcgccag gccatcatca tcgacgagct gccgtatcag gtgaacaagg cgcagctcct
      241 cgtgaagatc ggcgagctgg tgcgcgagaa gcgcatcgag ggcatctcgg acctgcgcga
      301 cgaatccgac aagtccggca tgcgcgcggt gatcgagctg aagcgcggcg aggtcgccga
      361 gatcgtgctg aacaatctct acaagctcac gccgatgcag gaaagcttcg gcatgaacat
      421 ggtcgcgctc ctcgacggcc agcccaagct cctcaacctg aagcagttcc tcgatgcgtt
      481 cctgcgccat cgccgcgagg tcgtcacgcg ccgcaccgtg ttcgacctgc gcaaggcgcg
      541 cgaccgcggc cacatcctgg aaggtctcgc cgtcgcgctc tccaacgtgg acgaagtgat
      601 cgcgatcatc aagggcgcgg cgacgccggc ggtcgcgaag accgggctca tggcgcgcgc
      661 gtggcgctcg cccctggtcg agcagatgct ggcgcgtgcc accgccgagc aataccggcc
      721 cgaggcgctg cccaaggaat tcggcctggt gaaggattcg cccgacgcgg cgggctaccg
      781 gctctccgag acgcaggcgc agcgcatcct cgagatgcag ctgcagcgct tgaccggcct
      841 cgagcaggac aagatccttt ccgagtaccg cgaagtcatc gccaccgtcg aggatctcct
      901 cgacatcctc gccaagcccg agcgcgtgac gaggatcatc ggcgacgagc tcgccgaggt
      961 gaagcgcgag ttcggcgaca agcgccgcag cgagatcgtc gtgaatgggg aggagctctc
     1021 tctcgaggat ctgatcaccc ccgaggacgt ggtcgtcacg ttgtcccatg cgggttacgt
     1081 gaagagccag cccctcgacg agtaccgcgc gcagaagcgc ggcgggcgcg gcaagcaggc
     1141 gaccaccacc aaggaagacg atttcgtcga gaagctcttc atcgccaaca cgcacgacca
     1201 cgtgctgtgc ttctccaaca agggcaaggc ctactggctc aaggtctacg aggtgccgca
     1261 gggcagccgc accagccgcg gcaagcccat cgtgaacctc ctcaagatgg acgaggacga
     1321 gcgcatcaac gcgatcctgc cggtgaagga gttccgcgag gattgctacg tgttcttcgc
     1381 gacccaggaa ggcgtcgtga agaagacgtc cctctcggac tactcgcgcc cgcgcccctc
     1441 cggcatcatc gcggtggatc tcgacgaggg cgatcgcctc atcggcgtgg ccctcaccga
     1501 cggcaagcac gacgtgatgc tcttctcgga cgaaggcaag gcggtgcgct tcgacgagaa
     1561 cgatgtgcgc cccatgggcc gcggcgcgcg cggcgtgcgc ggcatgaata tcgccaaggg
     1621 cggcaaggtg atcgcgctgc tggtggccg
//
LOCUS       DASVII010000374         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_556958,
            whole genome shotgun sequence.
ACCESSION   DASVII010000374 DASVII010000000
VERSION     DASVII010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556958"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VF309_04900"
     CDS             <1..1012
                     /locus_tag="VF309_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7465960.1"
                     /translation="QLFKGDAPDLDLYHPWGLTVEEAIEIARRTEGAALAVDERITNS
                     EGGTVSAYDTDFILANTRGFLAGYPSSKASISVGVVAEDAGGMQRDYWFATHRDPQRL
                     DNPELIGRIAGERAVRRLGGRRLPTTEAPVLFDANIAGSLIGHFVSAASGSSLYRRSS
                     FLLDRLGTPVFSPVVQIVEDPHLPGEIASSYYDAEGVITRSRVIVAAGVLEGWFLSTY
                     SARKLGLETTGNAGGNHNLIMKPGALDLEGLVKRMGRGLLVTDLMGQGVNTVTGDYSR
                     GAAGFWVEGGEIRFPVEEITIAGNLLDMFKGIEEVGRDVLVRGSKQNGSILVSRMTIA
                     GD"
     gene            1092..2183
                     /locus_tag="VF309_04905"
     CDS             1092..2183
                     /locus_tag="VF309_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7465961.1"
                     /translation="MKRRQFINTTGAGMAGILAAGVAPAFAQGAEIKWRLASSFPKSL
                     DTIYGAAEVMSKRVAACTGGKFQIQVFSAGEIVPAFGVVDAVQNGTVQAAHTAPYYFW
                     GKDPAFALDTAIPFSLNARQTNAWQIFGGGLELFRDFYKAYNIYNVPCGNTGAQMGGW
                     FRKEIKTPADWKGLKFRIGGFAGKVIEKMGGVPQLIPGGDIYPALEKGTIDAAEWVGP
                     YDDEKLGFNKIAKYYYYPGWWEGGPQLSAFFNMAEWAKLPKEYQNIVEAACWEANTWM
                     LAKYDAQNPAALKRLIGGGTQLKAFSKDVMVASYKAALEVYKETEEKSPAFKKIYKSM
                     TEFREEQLLWFRVAEKGYDDFMHTIGTIK"
BASE COUNT          432 a          770 c          816 g          374 t
ORIGIN      
        1 ccagctcttc aagggcgacg cgcccgatct cgatctctac catccgtggg gcctcacggt
       61 ggaggaggcg atcgagatcg cgcggcgcac cgagggcgcg gcgctcgcgg tcgacgagcg
      121 catcaccaac tccgagggcg ggacggtctc ggcgtacgac accgacttca tcctcgcgaa
      181 cacccgcggc ttccttgccg gctatcccag ctccaaggcg agcatcagcg taggcgtcgt
      241 cgcggaggat gccggaggca tgcaacggga ctactggttc gcgacacacc gcgatccgca
      301 gcgcctcgac aatcccgagc tcatcgggcg catcgcgggc gagcgcgccg tgcggcgcct
      361 cggggggcgc cgcctcccga cgaccgaagc gccggtgctc ttcgacgcga acattgccgg
      421 aagcctgatc ggccacttcg tttccgcggc gagcggctcg agcctgtatc gccgctcgtc
      481 gttcctgctg gaccggctcg gcacgccggt gttctcgccg gtcgtccaga tcgtcgagga
      541 tccgcacctg ccgggcgaga tcgcgagcag ctattacgac gcggaagggg tgattacgcg
      601 ctcccgcgta atcgtcgcgg cgggcgtgct cgagggctgg ttcctgtcga cgtattcggc
      661 gcgcaagctc gggctcgaga cgaccggcaa tgcgggcggc aaccacaacc tcatcatgaa
      721 gcccggcgcg ctcgacctcg aggggctggt gaagcgcatg ggccgcgggc ttctcgtgac
      781 cgatctcatg ggccaggggg tcaataccgt gaccggcgac tactcgcgcg gcgcggcggg
      841 gttctgggtg gaaggcggcg agatccgctt ccccgtcgag gagatcacga tcgccggcaa
      901 tctcctggac atgttcaagg gcatcgagga agtgggccgc gacgtgctcg tgcgcggctc
      961 caagcagaac gggtccatcc tcgtcagtcg gatgacgatt gccggcgact agcttgatat
     1021 tttgctaggc tagcggcgca tcgtgcacag gcgttcgcgc agcggacccg aacaattcca
     1081 gaggagatcg catgaaacgc aggcaattca tcaacaccac cggagccggc atggcgggca
     1141 ttctcgccgc gggcgtggcg ccggcattcg cgcagggcgc ggagatcaag tggcggctcg
     1201 catctagctt tccgaagtcc ctcgacacga tctacggcgc ggccgaggtc atgtccaagc
     1261 gcgtggcggc atgcacgggc ggcaagttcc agatccaggt gttctccgcg ggcgagatcg
     1321 tcccggcctt cggggtcgtc gatgcggtgc agaacggcac cgtgcaggcg gcccacaccg
     1381 ccccctatta cttctggggc aaggaccccg ccttcgcgct ggataccgca attccattca
     1441 gcctgaacgc gcgccagacc aacgcctggc agattttcgg cggcggcctc gagctcttcc
     1501 gcgacttcta caaggcctac aacatctaca acgtcccgtg cggcaacacg ggagcgcaga
     1561 tgggcggctg gtttcgcaag gagatcaaga cgccggcgga ctggaagggc ctgaagttcc
     1621 gcatcggggg cttcgcgggc aaggtgatcg agaagatggg cggcgtgccg cagctcattc
     1681 ccggcggcga catctacccg gcgctcgaga agggcaccat cgacgcggcg gaatgggtcg
     1741 gcccctacga cgacgagaag ctcggcttca acaagatcgc caagtactac tactaccccg
     1801 gctggtggga gggcggcccg cagctgagcg cgttcttcaa catggccgag tgggcgaagc
     1861 tccccaagga gtaccagaac atcgtcgagg cggcgtgctg ggaagccaac acctggatgc
     1921 tcgccaagta cgacgcgcag aaccccgcgg ctctgaagcg cctgatcggc gggggaacgc
     1981 agctgaaggc gttctcgaag gatgtcatgg tcgcctccta caaggcggcg ctcgaggtct
     2041 acaaggaaac cgaggagaag agcccggcgt tcaagaagat ctacaagagc atgaccgagt
     2101 tccgcgaaga gcagctgctg tggttccgcg tggccgagaa gggctacgac gacttcatgc
     2161 acaccatcgg caccatcaag tagcgaggcc gttgcgaaga aagggcccgc cgtcgcgggc
     2221 ccttttcatt ttcggggctc ggcatcgcgc tccagctgct tgtccagcat ctcgcgcagg
     2281 gcgtcgttgg aaggcgtcgc ggcggcgggg agagcccgcg ccgggcttcc ggcgtcgcgc
     2341 cacagcattc cggggaaggc gatcacgagc gcgacgacga cgagctgcgc cg
//
LOCUS       DASVII010000375         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_53490, whole
            genome shotgun sequence.
ACCESSION   DASVII010000375 DASVII010000000
VERSION     DASVII010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53490"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="hslU"
                     /locus_tag="VF309_04910"
     CDS             <1..957
                     /gene="hslU"
                     /locus_tag="VF309_04910"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX7465962.1"
                     /translation="AQNAEERLLDVLLPPAREVNIQDMQPTDSATRQKFRKMLREGKL
                     DEREVEIEVTLQAPQMEMMAPPGMEELTSQIQGMFANIGGTRRKRRTLRIREALRLLV
                     DEEALKLVNEEEIRFKALRAVEEDGIVFLDEIDKIASRGELHGADVSRQGVQRDLLPL
                     VEGTAVTTKYGMIRTDHILFIASGAFHLAKPSDLIPELQGRLPIRVELASLSVEDFER
                     ILTATDACLTRQYQALLATEGVELVYTPDGVRRLAEIAFAVNERTENIGARRLYTVMQ
                     KLLEEVSFDAGRKSARITIDAAYVDDRLGELAKSEDLARYVL"
     gene            1004..>1784
                     /locus_tag="VF309_04915"
     CDS             1004..>1784
                     /locus_tag="VF309_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7465963.1"
                     /translation="MRHASTPLALPLALLLAACANSPSAPPDPGASLAAAETEFAALS
                     VREDMRAAFLANFADDGVYVRDGWVEAKADLAKRSAPAIVLDWKPVFTEVAWSGDLGL
                     STGPWKITSKAKPDAAPSFGQFVSVWRRDPGGPWKVVVDIGIAHAGAALWDQPLATVM
                     LPGRAALTRGGIESAELDFVSDAHLKGLPAAYATYGSERFRFYRDGFEPAVGKANALA
                     SPAMTGEKLAWSIDQIEMSRARDFGYSRGSYAAVATPKTPLG"
BASE COUNT          289 a          637 c          602 g          256 t
ORIGIN      
        1 gcccagaacg ccgaagagcg gctcctcgac gtgctgctgc cgccggcgcg cgaggtcaat
       61 atccaggaca tgcagcccac ggattccgcg acgcgtcaga agtttcgcaa gatgctgcgc
      121 gaaggcaagc tcgacgagcg ggaagtcgag atcgaggtca cgctgcaggc gccgcagatg
      181 gagatgatgg cgccgccggg aatggaggag ctcacgagcc agatccaggg catgttcgcc
      241 aacatcggcg gcacgcggcg caagcgccgc acgctgagga tccgcgaggc gctgcgcctg
      301 ctcgtcgacg aggaggcgtt gaagctggtg aacgaggagg agatccgctt caaggcgctg
      361 cgcgcggtcg aggaggacgg catcgtgttc ctcgacgaga tcgacaagat cgcgagccgc
      421 ggcgagctgc acggcgccga cgtctcgcgc cagggcgtgc agcgcgacct gctgccgctg
      481 gtcgagggca ccgcggtcac gaccaagtac ggcatgatcc gcaccgacca catcctcttc
      541 atcgcgagcg gcgcgttcca cctggccaag ccctcggacc tcattcccga gctgcaggga
      601 cggcttccga tccgcgtcga gctcgcgagc ctctcggtcg aggacttcga gcgcatcctc
      661 accgccaccg acgcgtgcct cacgcggcag taccaggcgc tcctcgccac cgagggcgtg
      721 gagctggtgt acacccccga cggcgtgcgg cgcctggccg agatcgcctt cgcggtgaac
      781 gagcgcaccg agaacatcgg cgcccggcgc ctgtacacgg tgatgcagaa gctcctcgag
      841 gaagtctcct tcgacgcggg ccgcaagtcc gcgcgcatca ccatcgacgc ggcctacgtc
      901 gacgaccgcc tcggcgagct cgccaagagc gaggatctgg cgcgctacgt gctgtgaaag
      961 gtttggtaac ggtttcgccg gcgtgacggc ctagaatcgc ctcatgcgcc acgcgtccac
     1021 gcccctcgcg ctgcctctcg cgctgcttct cgcggcctgc gccaactcgc catcggcgcc
     1081 ccccgatccc ggcgcctcgc tcgccgcggc cgaaaccgag ttcgccgcgc tctcggtgcg
     1141 cgaggacatg cgcgccgcct tcctcgcgaa cttcgccgac gacggcgtct acgtgcgcga
     1201 cggctgggtc gaggcgaagg cggatctcgc gaagcgaagc gcccccgcga tcgtgctcga
     1261 ctggaagccg gtcttcaccg aagttgcgtg gtccggcgac ctcggcctgt cgacggggcc
     1321 gtggaagatc accagcaagg cgaagcccga cgccgcgcct tccttcggcc aattcgtctc
     1381 ggtctggcgg cgcgacccgg gcggaccgtg gaaggtcgtc gtcgacatcg gcatcgcgca
     1441 cgccggcgca gccctctggg accagcccct ggcgaccgtg atgcttcccg gccgcgcggc
     1501 cctcacgcgc ggcggcatcg aatccgcgga gctcgatttc gtgagcgacg cgcacctgaa
     1561 gggcctcccc gccgcgtacg cgacctacgg ctcggagcgg tttcgcttct atcgcgacgg
     1621 gttcgagccc gccgtcggaa aagccaacgc gctcgcctcg cccgcgatga ccggcgagaa
     1681 gctcgcctgg tccatcgacc agatcgagat gtcgcgcgcg agggacttcg gctattcgcg
     1741 cggcagctac gcggccgtgg caacgcccaa gacgccgctc ggat
//
LOCUS       DASVII010000376         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_520728,
            whole genome shotgun sequence.
ACCESSION   DASVII010000376 DASVII010000000
VERSION     DASVII010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520728"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VF309_04920"
     CDS             <1..951
                     /locus_tag="VF309_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465964.1"
                     /translation="IHVYDAPPIAQQGLSSARRYADIAPDAPHALHMPSHIFTRVGSW
                     TESAATNERSAAAAKNDRDPSEQLHAMDYLVYAYLQLARDADARGAIDDAMKVAGASP
                     RFTAPYAMSAMPARYALERGAWQEAANLKAPGTSYPFVEAITYFARALGAARSGDLAA
                     ARKDAEQLETFHNALLAAKNTYWATEVEIQQLAAAGWIALGEGKTDEALKFMRAAADL
                     EDRNEKHIVTPGRVVPARELLGEMLLEVKQPEAALKEFEASQRREPNRFRNYLGSARA
                     ADMAGDRTKAAAYYQKLIALAKNSDSERPELARAKKYAQS"
     gene            948..1439
                     /locus_tag="VF309_04925"
     CDS             948..1439
                     /locus_tag="VF309_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7465965.1"
                     /translation="MRGFAGLVRASLAVAAIATFTPAATFAPIAAADPFEANPDLATR
                     DEDYAAGKKAIEKKDWAEAARLFKLSERRNPDSADLQNYLGYAYRNQKKFDLAFRHYE
                     RAIELDPRHRGAHEYIGEAYLMTGNLAGAEKHLAALKEICLLPCEELKDLERAVAVYR
                     RGK"
     gene            complement(1446..1652)
                     /locus_tag="VF309_04930"
     CDS             complement(1446..1652)
                     /locus_tag="VF309_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX7465966.1"
                     /translation="MVTGVVRSFNDAKGFGLITPDLGGPPIFVHQSAIRTPGVKKLKP
                     TQRVEYEVEIRPEGPAASNLRMLA"
     gene            complement(1670..1873)
                     /locus_tag="VF309_04935"
     CDS             complement(1670..1873)
                     /locus_tag="VF309_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7465967.1"
                     /translation="MAAGTVKWFNDAKGFGFITPDDGGKDLFAHHSEIQGTGFKSLKE
                     GQKVEYEATQGAKGPAASKIRPV"
BASE COUNT          388 a          732 c          717 g          341 t
ORIGIN      
        1 attcacgtct acgacgctcc gccgatcgcg cagcaggggc tctcctccgc gcgacggtat
       61 gcggacatcg cgcccgacgc gccacacgcg ctgcacatgc cctcgcacat cttcacccgc
      121 gtcggctcat ggaccgagtc cgcagccacc aacgagcgca gcgcggcggc cgcgaagaac
      181 gaccgcgacc ccagcgagca gctgcacgcg atggactacc tggtctacgc gtacctgcag
      241 ctcgcgcgcg atgccgacgc tcgcggcgcc atcgacgacg cgatgaaggt ggccggcgcg
      301 agcccgcgct ttaccgcgcc ttacgccatg agcgccatgc cggcgcgcta cgccctcgag
      361 cgcggcgcgt ggcaggaggc ggcgaatctg aaggcgccgg gcaccagcta tcccttcgtc
      421 gaggccatca cctacttcgc gcgcgcgctc ggcgcggcgc gcagcggcga cctggccgcg
      481 gcccgcaagg atgccgagca gctcgagacc ttccacaacg cgttgctcgc cgcgaagaat
      541 acctactggg ccaccgaggt cgagatccag cagctggccg cggccggatg gatcgcgctg
      601 ggagagggca agaccgacga ggcgctgaaa ttcatgcgcg cggcggcgga tctcgaggat
      661 cgcaacgaga agcacatcgt gacgccaggc cgcgtcgttc cggcgcgcga gctgctgggc
      721 gagatgctcc ttgaggtgaa gcagcccgag gcggcgttga aggagttcga ggcctcgcag
      781 cgccgagagc ccaatcgctt ccgcaactac ctgggatccg cgcgcgccgc ggacatggcg
      841 ggcgaccgga cgaaggcggc cgcctactat cagaagctca tcgcgcttgc gaagaattcc
      901 gactccgagc ggccggaact ggcgagagcg aagaagtacg cgcagtcgtg agggggttcg
      961 ccgggctggt tcgggcgagc ctcgccgtcg cggcgatcgc gaccttcacc cctgccgcca
     1021 ccttcgcgcc gatcgccgcc gccgatccct tcgaggcgaa tcccgacctc gccacgcgcg
     1081 acgaagatta cgcggcgggc aagaaggcga tcgagaagaa ggactgggcg gaggccgcgc
     1141 ggctcttcaa gctttcggag cggcgcaatc ccgactccgc ggacctgcag aactacctcg
     1201 ggtatgcgta ccgcaaccag aagaagttcg acctcgcgtt ccggcactac gagcgcgcga
     1261 tcgagctcga tccgcgccac cggggcgcgc acgagtacat cggcgaggcc tacctcatga
     1321 cggggaacct cgcgggcgcg gaaaagcacc tcgcggcgct caaggagatc tgcctgctgc
     1381 cctgcgagga gctgaaggac ctcgagcgcg ccgtcgcggt gtaccggcgc gggaaataga
     1441 gccttttacg ccagcatgcg gaggttcgag gcggcggggc cctcgggacg gatctccact
     1501 tcgtactcca cacgctgcgt gggcttcaac ttcttcacgc cgggcgtgcg gatcgcggat
     1561 tgatgcacga agatcggcgg gccgccgaga tccggcgtga tgaggccgaa gcccttggcg
     1621 tcattgaacg acctgacgac acccgtgacg atcttcgcgg cgtcggccat cagacaggcc
     1681 ggatcttgga agcggcgggg cccttggcgc cttgcgtcgc ctcgtactcc accttctggc
     1741 cttccttgag ggatttgaag cccgttccct ggatttccga atgatgggcg aaaagatcct
     1801 tgccgccatc gtcgggggtg atgaatccga aacctttagc gtcgttgaac catttgacgg
     1861 tacctgcagc catctcgata tccttcctac tttgttcatg ggtgggaatg cgattgtacg
     1921 cgccctgcga atccctgccg ggttcgatag cgcacagatg gatttgggga acttccataa
     1981 gctggatgat cgaatccagg tccaatcgca agcgaggtat tttcgtgcac gtcattccca
     2041 ggattgccgt ggccctggcg gtaatttttt cgctcggagc ctgcaacacc cttcccaccg
     2101 ttgagcccga cgccggggcg aacgccgcgc ccgcgcgcct ggaaggcacg cgcggccccc
     2161 tgacggcgca gcagagca
//
LOCUS       DASVII010000377         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_377374,
            whole genome shotgun sequence.
ACCESSION   DASVII010000377 DASVII010000000
VERSION     DASVII010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377374"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VF309_04940"
     CDS             complement(<1..476)
                     /locus_tag="VF309_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7465968.1"
                     /translation="MKTKTLLQIQASIFSDGGESSRLASRFVSRWRADQPGADVIVRD
                     LGREPVPHLTAERFQSFLAKPDERTPAQRGVSDYSDNLIAELERADVIVLGLPMYNFG
                     VPSTLKAYFDHIARAGRTFKYTEKGPVGLLTGKKAFVFATRGGLYAGTPLDTQTSYV"
     gene            573..1499
                     /locus_tag="VF309_04945"
     CDS             573..1499
                     /locus_tag="VF309_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7465969.1"
                     /translation="MADTPRITLEQWRALQAVVEAGGYAQAAAALHKSQSTVTYAVQK
                     IESLLEVKLFEIRGRKAVLTGAGQVLYRRARTLLEEATLLERGAAAMAEAWQPEIRIA
                     VELIFPTWLLLECLAEFARERPETRIEIFETVLTGTDEMLAEGRVDIAIGSLAAGVTS
                     TALMPVRFIAVAHPGHALHRLKRPLTDRDLRRHRRIFIRDTGAQRKSDVAGVDLRWTV
                     SHKATSIRAVAMGLGFAWLPEETIQPELRDGTLKPLPMRGGSERFAQLYLSLSDPEFP
                     GRDAARLVEIIRAKVAAQCMHDRKKVRARRRG"
     gene            1496..>1648
                     /locus_tag="VF309_04950"
     CDS             1496..>1648
                     /locus_tag="VF309_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7465970.1"
                     /translation="MTARTHDVIVVGAGAAGMMCAATAGQRGRHVVLVDHYAKVGEKI
                     RISGGGR"
BASE COUNT          257 a          548 c          588 g          255 t
ORIGIN      
        1 acgtagctcg tctgggtgtc gagcggcgtg ccggcgtaga gcccgccgcg cgtcgcgaag
       61 acgaacgcct tcttgccggt gaggagcccc acgggcccct tctcggtgta cttgaaggtc
      121 ctgcccgcgc gcgcgatgtg gtcgaaatag gccttgagcg tcgagggcac tccgaagttg
      181 tacatcggca gccccagcac gatcacgtcg gcgcgctcga gctcggcgat caggttgtcg
      241 gaatagtccg acacgccgcg ctgcgccggc gtgcgctcgt cgggcttggc gaggaacgac
      301 tggaagcgct ccgcggtgag gtgcgggacc ggctcgcggc caaggtcgcg cacgatcacg
      361 tcggcgcccg gctggtccgc tctccagcgg gaaacgaagc ggctcgccag gcggctcgac
      421 tcgccgccgt cggaaaagat gctcgcctgg atctgcagga gcgttttcgt cttcatgggt
      481 atctccgtgg aatggctcat gccccaatct acggtttacc gagtcgatta aaaagcgcaa
      541 aatttgccac ttatccatcg aattaatcga tcatggcgga caccccgcgg atcaccctcg
      601 agcagtggcg cgcgctgcag gcggtcgtcg aggccggggg ctacgcccag gccgccgcgg
      661 cgctccacaa gagccagtcg accgtcacct acgcggtgca gaagatcgag tccctcctcg
      721 aggtgaagct cttcgagatc cgcggccgca aggcggtgct caccggggcg gggcaggtgc
      781 tctaccggcg cgcgcgaacg ctgctcgagg aggcgacgct tctcgagcgc ggcgcggccg
      841 ccatggccga ggcgtggcag ccggagatcc gcatcgcggt ggagctcatc ttcccgacgt
      901 ggctcctcct cgagtgcctc gcggaattcg cgcgcgagcg gcccgagacg cgcatcgaga
      961 tcttcgagac ggtgctcacg ggaaccgacg agatgctcgc ggaggggcgc gtcgacatcg
     1021 ccatcggctc gctcgcggcg ggcgtcacga gcacggcgct catgccggtg cgcttcatcg
     1081 ccgtcgccca tcccggccat gcgctgcacc ggctcaagcg cccgctcacg gatcgcgacc
     1141 tgcggcgcca ccggcgcatc ttcattcgcg acaccggcgc gcagaggaag agcgacgtcg
     1201 ccggcgtgga tctgcgatgg accgtgagcc acaaggcgac ctcgatccgc gccgtcgcga
     1261 tgggcctcgg gttcgcatgg cttcccgagg agacgatcca gccggagctg cgcgatggga
     1321 cgttgaagcc gctgccgatg cgcggcggct ccgagcgctt cgcgcaactc tatctgtcgt
     1381 tgtccgaccc ggagtttccc ggtcgcgacg ccgcgcggct ggtcgagatc attcgcgcga
     1441 aggtcgccgc gcaatgcatg cacgaccgga agaaggtccg ggcgcggcgg cgcggatgac
     1501 ggcgcgaacg cacgacgtga tcgtggtcgg cgcgggcgcc gcggggatga tgtgcgccgc
     1561 caccgcgggt cagcgcgggc gccacgtggt gctggtggat cactacgcga aggtcggcga
     1621 gaagatccgc atttccgggg gcgggcgc
//
LOCUS       DASVII010000378         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_89084, whole
            genome shotgun sequence.
ACCESSION   DASVII010000378 DASVII010000000
VERSION     DASVII010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89084"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF309_04955"
     CDS             complement(<1..216)
                     /locus_tag="VF309_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7465971.1"
                     /translation="MLAVAFATSAGATFKCVDPKGVTRIGDTPPEECGNVVVYEISAG
                     GAVIKKIEPTPTPEQVKALEEDRERKKE"
     gene            complement(267..950)
                     /gene="pyrE"
                     /locus_tag="VF309_04960"
     CDS             complement(267..950)
                     /gene="pyrE"
                     /locus_tag="VF309_04960"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7465972.1"
                     /translation="MTVAALSAEAPDRDAFRREFVDFAIRAGVLRFGEFKTKAGRLSP
                     YFFNAGLFCDGAKLGELAGFYARAALASGIRFDLIFGPAYKGITLAAATAVALAGLGH
                     NVPFSFNRKEAKDHGEGGNIVGAPLKGRVLVIDDVITDGASKREAIDLILEAGAVPAG
                     VLIALDRQERGQGELSATQEVSRDYGLPVAAIASVADIVATLRARPGEAGNVERIEAY
                     RKQYGVAQP"
     gene            1003..>1621
                     /locus_tag="VF309_04965"
     CDS             1003..>1621
                     /locus_tag="VF309_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7465973.1"
                     /translation="MLRIRDVTLRRGARVLLEGASMNVHPGQKVGLVGPNGSGKSSLF
                     ALVRGELHADAGEVDLPPRWVLSHVAQETPAVERAALEFVMDGDEELREIEREIAIAE
                     AAHEEGIHLANLHARYEEIGGYQARARGQTLLAGLGFDEAAQSRVVARFSGGWRMRLN
                     LARALMCRADLLLLDEPTNHLDLDAVLWLEDWLGAVPGAVILITHD"
BASE COUNT          249 a          546 c          561 g          265 t
ORIGIN      
        1 ctccttcttg cgctcccggt cttcctcaag cgccttcact tgctccggcg tcggagtggg
       61 ctcgatcttc ttgatgacgg cgccgcccgc gctgatctcg tagacgacga cgttgccgca
      121 ctcttccgga ggcgtgtccc cgatccgcgt gacgcccttg gggtcgacgc acttgaacgt
      181 ggccccggcc gacgtggcga atgcgaccgc caacaacaga aatattcttc tcattttctt
      241 ctctcccaac gcgcgccttg ttttcatcat ggttgagcca ctccgtactg ttttcggtag
      301 gcctcgatac gctcgacgtt gcccgcctcg ccgggacggg cgcgcagtgt cgcgacgatg
      361 tccgcgacgc tcgcgatggc ggcaacgggc aagccgtagt cgcggctgac ttcctgcgtc
      421 gccgagagct cgccctgccc gcgttcctgg cgatccagag caattagcac gccagccgga
      481 accgctcccg cctcgaggat gaggtcgatc gcctcgcgct tcgaggcgcc gtccgtgatc
      541 acgtcgtcga tgacgaggac gcgtcccttc aggggagcgc cgacgatgtt gccgccctcg
      601 ccatggtcct tcgcttcctt gcggttgaac gagaacggca cattgtgacc gagaccggca
      661 agggccaccg ccgtcgccgc cgccagcgtg atgcccttgt acgcgggccc gaagatcagg
      721 tcgaagcgga tcccggaggc cagggccgcg cgggcgtaga agccggcgag ctcgcccagc
      781 ttcgcgccgt cgcagaagag gcccgcgttg aagaagtacg gcgagaggcg ccccgccttg
      841 gtcttgaact cgccgaagcg aagcaccccc gcccggatgg cgaagtcgac gaactcgcgg
      901 cggaacgcgt cgcggtcggg ggcttcggcg gagagggcgg caacggtcat gggcgagtcc
      961 gggggtacga tagccgctat tttcgctcaa agcatctgaa gcgtgctgag aatcagggat
     1021 gtgacgttgc gccgcggcgc gcgagtgctg ctcgagggcg cgtcgatgaa cgtgcacccc
     1081 gggcagaagg tcgggctcgt ggggcccaac ggcagcggca agtcgagcct tttcgcgctg
     1141 gtgcggggcg agctgcacgc ggacgcgggc gaggtcgacc tgccgccgcg ctgggtcctg
     1201 tcccacgtgg cgcaggaaac gcccgccgtc gagcgcgcgg cgctcgagtt cgtgatggac
     1261 ggcgacgagg agctgcgcga gatcgagcgc gagatcgcca tcgcggaagc cgcgcacgag
     1321 gagggaatcc acctcgcgaa cctgcacgcg cgctacgaag agatcggcgg ataccaggcg
     1381 agggcccgcg ggcagacgct gctcgccggc ctcggattcg acgaagccgc gcagtcccgg
     1441 gtggtggcgc ggttctcggg cggctggcgc atgcggttga acctcgcgcg cgccctcatg
     1501 tgccgcgccg acctgctgct gctggacgag cccaccaacc acctcgacct cgacgcggtg
     1561 ctatggctcg aggactggct cggggcggtt cccggggcgg tgatcctcat cacccacgac
     1621 c
//
LOCUS       DASVII010000379         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_413300,
            whole genome shotgun sequence.
ACCESSION   DASVII010000379 DASVII010000000
VERSION     DASVII010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413300"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..822)
                     /locus_tag="VF309_04970"
     CDS             complement(115..822)
                     /locus_tag="VF309_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7465974.1"
                     /translation="MSLPQEQSLDPNMPDAELAKCVSGGSEPALRILMRRYNQALFRT
                     ARSILRDDAEAEDALQDAYMAAYRAMGAFRGDSKLATWLTRIVVNESLARLRKSRRGA
                     QVIPIDGDADAALAIAEIRMSEESPNPTESDAIGAQSRKLIEAKIDELPDVFRTVFVM
                     RAVEDMTIEEIAASLDIPEATVRTRFFRARGLMREALAREADRAVASAFSFAGERCDR
                     IVERVIAEFRKIQSPQG"
     gene            991..1314
                     /locus_tag="VF309_04975"
     CDS             991..1314
                     /locus_tag="VF309_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7465975.1"
                     /translation="MNRLLAAALLPLSLAALGAGEPEINVVIEGHKFTPDRIEVPAGQ
                     KVRLLVENKDATPEEFESGSLKIEKVIPGKSKATIFVGPLKAGEYKFVGEFNEKTANG
                     VIVAK"
     gene            1320..>1840
                     /locus_tag="VF309_04980"
     CDS             1320..>1840
                     /locus_tag="VF309_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX7465976.1"
                     /translation="MFATALIVFREVLEAALIVGIVAAATRGIRGRGQWIGAGIGAGL
                     AGALFVAVGAKAISQLAQGMGQELFNASILGIAVAMLAWHNIGMSIHGRELSKSARDV
                     SNEIRSGAKELSILMVVVAVAVLREGSETVLFLYGIAVSGNSSAAPMAIGAAIGLAGG
                     VAVGWFLFAGLLR"
BASE COUNT          294 a          582 c          637 g          327 t
ORIGIN      
        1 aaagcatgag gccgtggccg atgaacctgc ggcgcgcggg aagcgcgagc ggtgaatcga
       61 tgaaatcgag gaatgaggac attgggtttc tttctccttg ggtgtgtcgg acggtcagcc
      121 ctgcggcgac tggatcttgc gaaactcggc gatgacgcgc tcgacgatgc gatcgcagcg
      181 ctcgccggcg aacgagaagg cgctcgcgac ggcgcggtcc gcctcccggg ccagcgcttc
      241 gcgcatgagg ccgcgcgcgc ggaagaagcg cgtgcgaacg gtcgcctcgg ggatgtcgag
      301 gctcgccgcg atctcctcga tcgtcatgtc ctcgacggcg cgcatcacga agaccgtgcg
      361 gaagacgtcc ggaagctcgt cgatcttcgc ttcgatgagc tttcgcgatt gcgcgccgat
      421 ggcgtcgctc tcggtggggt tgggcgattc ctcgctcatg cgtatctccg cgattgcgag
      481 cgccgcatcg gcgtctccat cgatgggtat gacctgggcg ccgcgccgcg acttgcgaag
      541 gcgcgcgagg gactcgttca ccacgatccg cgtaagccag gtcgcgagct tcgaatcgcc
      601 gcggaaagcg cccatggccc gataggccgc catgtaggcg tcctgcagcg cgtcctcggc
      661 ttcggcgtcg tcgcgcagga tgctgcgcgc cgtgcggaag agcgcctggt tgtagcggcg
      721 catcaggatg cgcaacgccg gctcgctgcc gccggagacg cacttcgcga gctccgcgtc
      781 cggcatgttg gggtcgagcg attgctcctg cggcaaggac atggatgcgt ttctccctct
      841 caggccgcat tagatgaagg ccggcctcgc ggcgttcctg caggcggccg gcaggcgcag
      901 cgggtccatt gttggacccc tattgcaatt cttaataaga acgattatca tttgcctgtc
      961 gagtccacca aggactcccg gagcctcgtg atgaatcgcc ttctcgccgc cgcccttctc
     1021 ccgctctcgc tcgccgccct cggggccggc gagcccgaga tcaacgtcgt catcgagggc
     1081 cacaagttca ctccggaccg catcgaggtg cccgccgggc agaaggtgag gctcctcgtg
     1141 gagaacaagg acgcgacgcc ggaggaattc gaaagcggat cgctcaagat cgagaaggtg
     1201 atccccggca agtcgaaggc gacgatcttc gtgggtccgt tgaaggccgg cgaatacaag
     1261 ttcgtcggcg agttcaacga gaagaccgcc aacggcgtga tcgtcgcgaa ataacttcca
     1321 tgttcgccac cgcgctcatc gtcttccgcg aagtcctcga ggcggcgctc atcgtcggca
     1381 tcgtggcggc ggcgacgcgc ggcattcggg gccgcgggca atggatcggc gcgggaatcg
     1441 gggccggcct cgccggcgcg ctcttcgtgg ccgtaggcgc gaaggcgatc tcgcagctcg
     1501 cgcagggcat gggccaggag cttttcaacg cgtcgatcct cggcatagcc gtggcgatgc
     1561 tcgcgtggca caacatcggg atgtcgatcc acgggcgcga gctctcgaag agcgcgcgtg
     1621 acgtgagcaa cgagatccgc agcggcgcga aggagctctc gattctcatg gtggtcgtcg
     1681 ccgtcgccgt tctgcgcgaa ggctccgaga cggtgctctt cctctatggc atcgccgtct
     1741 cgggcaactc ctcggcggca ccgatggcga tcggggccgc gatcgggctc gcgggtggcg
     1801 tcgccgtggg ctggttcctc ttcgcggggc tactgcgcat
//
LOCUS       DASVII010000380         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_557192,
            whole genome shotgun sequence.
ACCESSION   DASVII010000380 DASVII010000000
VERSION     DASVII010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557192"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VF309_04985"
     CDS             <1..1079
                     /locus_tag="VF309_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7465977.1"
                     /translation="TESGIQLSFSVVATAEPGHHPASAVSADAGMGVAVAKFMVAAMG
                     GKLAIATRPSGDALYAFTLEFPVRPSPPTPTRATYVSLVGLPVLVVSGDPEQRLQITN
                     LLRGWRLIPLEADNAAMAMALLERLREEGSPIPLVILANRLPVQDGFLLAFRIKNDGK
                     FRSALVMMLASEGRPGDAIACRENGISAYMRYPINDRQLNEAIVAVTGASVDSDETPT
                     LVTRHSLREQRKGATILLVDPSRDSQILAAHILGREDCSVVVAQDLTEALAALDQDVY
                     DIILVDTSLAGLGGDDAAELLRSRITRDPDKASFVAASLEHSPAYRAAKTAIGFNATV
                     AKPFRRDDLLALLAAVGRLPVEAD"
     gene            1091..2680
                     /locus_tag="VF309_04990"
     CDS             1091..2680
                     /locus_tag="VF309_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465978.1"
                     /translation="MVVARWRWPFALAACVAAAGFASVASRLDVNWDLKNYHLYNAYA
                     FLNGRLGWDIAPAQLQTYHNPLLDLPFYYLVQAIPSPRVIAFVMASTAGVAAFCLLRI
                     LAALFPKGFEDRALWIALAFIAGMTGSMGRSVIGSTMNEWPPAMLMMLSLAILVAPTA
                     ARASPRARDVALASLLTGFAVGLKLTYGVFGAGLAISLAAFGTPRERLRRALLGGVFL
                     FAGFLATYGFWGATLYREFANPFFPYFNELFRSPWAEPVSFVDPNYGPREALQAIFFP
                     LYFARESRLVSEVSFRDWRLATLFVLAFLALARWLWSRRHGDAGAPRDLAATGHAWQL
                     LGAFTLASYLAWLGLFGIYRYLVPLEMLSAPLIVGCVLYIVPGRNARRALVVILVALL
                     VGTTRTASWGRLDFRGAYFDVSVPDLAPRALVIMGYAEPMSYAIPFARPDARFVYPWN
                     NFLQYDQHNLMAERARAVIGGHREAIYSMAFENSGNLAKLLDQYGLERDARSCLPIRS
                     YLDTSAIRLCRVYRKEDRHDR"
     gene            2670..>2883
                     /locus_tag="VF309_04995"
     CDS             2670..>2883
                     /locus_tag="VF309_04995"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7465979.1"
                     /translation="MTDENDAPLDGGCDCGEVRYRMTRPLFVNCCHCRWCQRETGSAF
                     AINAMIEADRVKLLKGTPEIVDTPSAS"
BASE COUNT          387 a         1048 c          918 g          530 t
ORIGIN      
        1 tgaccgaatc gggcatccag ctctcattca gcgtcgtcgc caccgccgag cccggccatc
       61 atcccgcctc cgccgtctcc gccgatgcgg gcatgggcgt cgcggtcgcg aaattcatgg
      121 tggccgcgat gggcggcaag ctcgccatcg cgacgcgccc ctcgggcgac gcgctctacg
      181 cgttcacgct ggaattcccg gtgcgcccct cgcctcccac gccgacgcgc gcgacctacg
      241 tctcgctcgt gggcctgccg gtgctcgtcg tctccggaga tcccgagcag cgcctgcaga
      301 taacgaacct gctgcgcggc tggcgcctca ttccgctcga agccgacaac gccgcgatgg
      361 cgatggcgct cctcgagcgg ctgcgcgagg aaggcagccc catcccgctg gtgatcctcg
      421 ccaaccgcct gccggtgcag gatggcttcc tcctcgcctt ccgcatcaag aacgacggca
      481 agttccgctc ggcgctggtg atgatgctcg cctccgaggg caggcccggc gacgccatcg
      541 cgtgccgcga gaacggcatc tcggcctaca tgcgctaccc gatcaacgac cggcagctga
      601 acgaggcgat cgtcgcggtc accggcgcct cggtggattc cgacgagacg ccgacgctcg
      661 tcacgcgcca ttcgcttcgc gagcagcgca agggcgcgac gatcctcctc gtggatccga
      721 gccgcgacag ccagatcctc gccgcgcaca tcctcggccg cgaggattgc agcgtggtgg
      781 tcgcccagga cctcacggag gcgctcgcgg ccctcgacca ggacgtctac gacatcatcc
      841 tcgtggacac gtcgctcgcc ggcctcggcg gagacgacgc cgccgagctc ctgcgctcgc
      901 gcatcacccg cgatcccgac aaggcgagct tcgtcgcggc gagcctcgag cactcgcccg
      961 cctaccgcgc ggcgaagacc gccatcggct tcaacgccac cgtcgccaag ccctttcgca
     1021 gggacgacct cctcgcgctc ctcgctgccg tggggcgcct ccccgtcgag gcggactgag
     1081 cttgcgagag ttggtcgttg cgcggtggag gtggccgttc gcgcttgcgg cgtgtgtcgc
     1141 ggcggcgggc ttcgcttcgg tcgcctctcg gctcgacgtc aattgggacc tcaagaacta
     1201 tcacctctac aacgcgtacg cgttcctcaa cgggcgactc gggtgggaca tcgcgcccgc
     1261 gcaacttcag acgtatcaca acccgctcct cgacctgccg ttctactacc tcgtccaggc
     1321 gattccctcg ccgcgcgtga tcgcgttcgt gatggcctcg acggcgggcg tggcggcctt
     1381 ctgcctcctg cggatcctgg cggcgctctt tccgaagggc ttcgaagatc gcgcgttatg
     1441 gatcgcgctc gcgttcatcg ccggcatgac gggctcgatg gggcgctcgg tcatcggctc
     1501 gacgatgaac gaatggccgc cggcgatgct catgatgctg tcgctcgcga tcctcgtggc
     1561 gccgaccgcg gcgcgcgctt ctccccgcgc gcgcgatgtc gcgctcgcgt cgctcctcac
     1621 gggcttcgcg gtgggcctca agctcaccta cggcgtgttc ggcgcgggac tcgcgatctc
     1681 gctcgccgcg ttcgggacgc cgcgcgagcg cctgcggcgc gcgcttctcg gcggggtctt
     1741 tcttttcgcc ggattcctcg cgacctacgg cttctggggc gcgacgctat atcgggaatt
     1801 cgcgaacccc ttctttccct acttcaacga gctcttccgc tcgccgtggg ccgagcccgt
     1861 ctcgttcgtc gacccgaact atggaccgcg cgaagcgctg caagcgatct tctttccgct
     1921 ctatttcgcg cgcgaatccc ggctcgtgag cgaggtgagc tttcgcgact ggcgcctcgc
     1981 gacgctcttc gtgctcgcgt ttctcgccct agcccggtgg ctgtggagcc gccgccacgg
     2041 ggatgccggc gcgcctcgcg atctcgccgc gaccgggcat gcgtggcagc tcctcggcgc
     2101 cttcaccctc gcgtcgtacc tcgcgtggct cgggcttttc ggcatctatc gctacctggt
     2161 gccgctcgag atgctgtccg cgccgctcat cgtgggctgc gtcctctaca tcgtgccggg
     2221 ccgcaacgcg cgccgcgcgc tggtcgtgat tctcgtggcg cttctcgtcg gcacgacgcg
     2281 cacggcgagc tggggtcggc tcgatttccg cggcgcctat ttcgacgtct cggttccaga
     2341 tcttgcgccg cgcgcgctcg tcatcatggg ctacgccgaa ccgatgtcgt atgcgatccc
     2401 gttcgcgcgc cccgatgcgc gcttcgtgta tccgtggaat aattttcttc agtacgacca
     2461 gcacaacctc atggcggagc gcgcgcgcgc ggtcatcggc gggcaccggg aagcgatcta
     2521 ctcaatggcc ttcgagaaca gcggcaacct ggcgaagctt cttgaccagt atggcctcga
     2581 gcgcgacgcg cgctcctgcc ttccgatccg ctcatatctc gatacgagcg caattcgcct
     2641 gtgccgcgtt taccgcaagg aggatcgcca tgaccgatga gaacgacgcg ccgctggacg
     2701 gtggctgcga ctgcggcgag gtgcgttacc ggatgacgcg gccgctcttc gtcaactgct
     2761 gccattgccg ctggtgccag cgcgagacgg gctcggcctt cgcgatcaac gcgatgatcg
     2821 aagcggaccg ggtgaagctg ctgaagggca cgcccgagat cgtcgacacg ccttcggcga
     2881 gcg
//
LOCUS       DASVII010000381         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_89151, whole
            genome shotgun sequence.
ACCESSION   DASVII010000381 DASVII010000000
VERSION     DASVII010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89151"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VF309_05000"
     CDS             <1..150
                     /locus_tag="VF309_05000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX7465980.1"
                     /translation="EQSARMVAMKAASDNAGNVIGELQLIYNKSRQAAITKELSEIVG
                     GAAAV"
     gene            248..1666
                     /gene="atpD"
                     /locus_tag="VF309_05005"
     CDS             248..1666
                     /gene="atpD"
                     /locus_tag="VF309_05005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7465981.1"
                     /translation="MNQGTIVQCIGAVVDVEFPRDQMPAIYDALKMDEIGLTLEVQQQ
                     LGDGIVRTIALGSSDGLRRGMTVVNTKNPILVPIGPKTLGRIMDVLGRPIDEAGPIGA
                     EKTSSIHRTAPKYEELSPSQDLLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKKHSGLSVFAGVGERTREGNDFYHEMIESGVIDKDNLQNSKVAMVYGQMNEP
                     PGNRLRVALTGLTMAEYFRDEKQASGKGRDVLLFIDNIYRYTLAGTEVSALLGRMPSA
                     VGYQPTLAEEMGRLQERITSTKKGSITSIQAVYVPADDLTDPSPATTFGHLDATVVLS
                     RDIASLGIYPAVDPLDSTSRQVDPNVIGEEHYNTTRAVQAVLQRYKELRDIIAILGMD
                     ELSPEDKLAVGRARKIQRFLSQPFHGAEVFTGSPGKYVTLKDTIKGFNMIVNGECDSM
                     PEQAFYMVGPIEEAFEKAKTLQ"
     gene            1688..>2083
                     /locus_tag="VF309_05010"
     CDS             1688..>2083
                     /locus_tag="VF309_05010"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX7465982.1"
                     /translation="MAKTLHVDIVSAEQQIFSGPAEMVIAPGEAGELGILPEHAPLLT
                     RIKPGTVRILQAGAEEEVIYVSGGMMEVQPDRVTVLADTSVRAHDLDEAKAMEAERLA
                     KEAIANRTGVMEIAKAQAELAEAAAQLAAI"
BASE COUNT          424 a          681 c          661 g          317 t
ORIGIN      
        1 gagcagagcg cgcgcatggt ggcgatgaaa gccgccagcg acaacgccgg caacgtgatc
       61 ggcgaactgc agctcattta caacaagtcg cgccaggcgg cgatcaccaa ggaactctcg
      121 gagatcgtcg gtggcgccgc agcggtatag gtttcgcctt tatgcaagat ccctcttttg
      181 tgcagatagt ttattcatct gcacaaaaga gggatccaaa cgaatctgat ctactaggaa
      241 tccagccatg aaccaaggaa caatcgttca gtgcatcggc gcggtagtcg acgtggaatt
      301 cccgcgcgac cagatgcccg ccatctacga cgcactcaag atggacgaga tcggcctgac
      361 cctcgaagtg cagcagcagc ttggcgacgg catcgtccgc accatcgcgc tcggctcgtc
      421 ggacggcctg cgccgcggca tgacggtggt caacacaaag aacccgatcc tggtcccgat
      481 cggcccgaag accctcggcc gcatcatgga cgtgctcggc cgcccgatcg acgaagccgg
      541 cccgatcggc gcggagaaaa cctcttcgat ccaccgcacg gcgcccaagt acgaagaact
      601 ctcgccttca caggacctgc tcgaaaccgg gatcaaggtg atcgacctgg tctgcccgtt
      661 cgccaagggc ggcaaggtgg gcctgttcgg cggcgccggc gtgggcaaga ccgtgaacat
      721 gatggagctg atcaacaaca tagcgaagaa acacagcggc ctgtcggtgt tcgccggcgt
      781 gggcgagcgc acgcgcgagg gcaacgactt ctatcacgag atgatcgagt cgggcgtcat
      841 cgacaaggac aacctgcaga attccaaagt cgcgatggtc tacggccaga tgaacgagcc
      901 gccgggcaac cgcctgcgcg tcgcgctcac cggcctcacg atggcggaat atttccgcga
      961 cgagaagcag gcgtccggca agggccgcga cgtgctgctc tttatcgaca acatctaccg
     1021 ctacacgctc gcgggcactg aggtgtcggc gctcctcggc cgcatgccct cggcggtcgg
     1081 ctaccagccg acgctcgcgg aggagatggg ccgcctgcag gagcgcatca cctcgaccaa
     1141 gaagggctcc atcacctcca ttcaagcggt ctacgttccc gcggacgacc tgacggatcc
     1201 gtcgcccgcg acgacattcg gccacctcga tgccacggtc gttctttcgc gcgacatcgc
     1261 gtcgctcggc atctaccctg cggtggaccc gctcgactcg acgtcgcgcc aggtggatcc
     1321 gaacgtgatc ggcgaggagc actacaacac gacgcgcgcg gttcaggcgg tgctgcagcg
     1381 ctacaaggag ctgcgcgaca tcatcgcgat cctgggcatg gacgagcttt cgcccgagga
     1441 caagctcgcg gtcggccgcg cgcgcaagat ccagcgcttc ctgtcgcagc ctttccacgg
     1501 cgcggaggtc ttcacgggct cgcccggcaa gtacgtgacg ctcaaggaca cgatcaaggg
     1561 cttcaacatg atcgtgaacg gcgagtgcga ctcgatgccg gagcaggcct tctacatggt
     1621 cggcccgatc gaggaagcgt tcgagaaggc gaagacgctc cagtaggcgc gtccagcgaa
     1681 agacaacatg gccaagacct tgcacgtcga catcgtcagc gcggaacagc agatcttttc
     1741 cgggccggcc gagatggtga tcgcgcccgg cgaggccggc gagctcggga tcctgcccga
     1801 gcacgcgccg ctcctcacgc ggatcaagcc gggaacggtg cgcatcctcc aggccggcgc
     1861 cgaggaggag gtgatctacg tctccggcgg catgatggaa gtgcagcccg accgcgtcac
     1921 ggtgctcgcg gacacgtcgg ttcgcgccca cgatctcgac gaggccaagg ccatggaggc
     1981 cgagcgcctc gcgaaggagg cgatcgccaa ccgtacgggc gtgatggaga tcgccaaggc
     2041 ccaggccgag cttgccgagg ccgccgccca gctcgccgcc atc
//
LOCUS       DASVII010000382         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_18066, whole
            genome shotgun sequence.
ACCESSION   DASVII010000382 DASVII010000000
VERSION     DASVII010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18066"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..234)
                     /locus_tag="VF309_05015"
     CDS             complement(82..234)
                     /locus_tag="VF309_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465983.1"
                     /translation="MRDWHRIESYTLTPDGKCPKCSTAIPGRFENFNASRQFGRRRIP
                     VAIGRS"
     gene            complement(410..1504)
                     /gene="amrS"
                     /locus_tag="VF309_05020"
     CDS             complement(410..1504)
                     /gene="amrS"
                     /locus_tag="VF309_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX7465984.1"
                     /translation="MPRPIEGPLHPGRWWHRLDDGRVQCDLCPRDCRLHEGQRGACFV
                     RARVGDAMVLTTYGRSSGFCVDPIEKKPLNHFLPGSAVLSFGTAGCNLACKFCQNWDI
                     SKSRDMDRLMDEASPEAIAIAAAESGCASVAFTYNDPVIFAEYAMDVADACREAGILT
                     VAVTAGYISPEPRREFYAKMDAANVDLKGFTDEFYVKLTGARLAPVLETLVYLEHETD
                     VWTEITTLLIPGRNDSDEELRAESRWIFRELGPDVPLHFTAFHPDFKMTDLPPTPAAT
                     LTRARRIAMDEGLRYVYTGNVHDKEGGTTFCHGCGAALIVRDWHRIEAYRLKANGCCP
                     DCGTKIPGRFEEFEISSQFGRRRIPVVIAR"
     gene            complement(1510..>1869)
                     /gene="amrA"
                     /locus_tag="VF309_05025"
     CDS             complement(1510..>1869)
                     /gene="amrA"
                     /locus_tag="VF309_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX7465985.1"
                     /translation="RPLGDDVARNAHAAAYRDPRFPPLQPDERASVAIEVSVLTPRVP
                     LAATSEDQALASLRPGEDGIYLQYLDMSATFLPQVWENLPEPADFLAELRRKAGLPAR
                     FWHPDLRLSRYTVEKYR"
BASE COUNT          312 a          615 c          624 g          318 t
ORIGIN      
        1 ccctatcggc gccatcttac gctgcggccc tccccttcaa cgtgtcgagc gtcaccccac
       61 ttcaatgagg cggtctgagg gctagctgcg cccgatggcg acgggaatgc gccgccgccc
      121 gaactggcgc gacgcgttga aattctcgaa ccgccccgga atcgccgtcg agcacttcgg
      181 gcactttccg tccggcgtga gcgtgtagct ttcgatgcgg tgccagtcgc gcacgatgag
      241 cgccgcgcgg ctgtcgttct accaggtgct catcgagcag caccgggagc ttcacgagca
      301 gcatccggag tggaaaaaat agcggggggt cagatcccaa ttcaagcgcg aggtcgactc
      361 gccagccgca ccggttcacg aaattgggct ctgaccccag cctcgagccc tatctggcga
      421 taacaaccgg aattcgtcga cgcccaaact ggctcgatat ctcgaactcc tcgaatcgcc
      481 ccggtatctt cgtgccgcag tccggacaac atccattcgc tttcagccgg tacgcctcga
      541 tgcgatgcca gtcgcgaacg atgagcgcgg cgccgcaacc gtggcagaac gtggtgccgc
      601 cttccttgtc gtggacgttg cccgtgtata cgtagcgcaa tccttcgtcc atcgcgatgc
      661 gccgcgcgcg cgtgagggtt gccgcgggcg tgggcggcag gtcggtcatc ttgaagtcgg
      721 gatggaacgc cgtgaaatgc aggggcacgt cggggcccag ctcgcggaat atccagcggc
      781 tctcggcgcg aagctcttcg tccgaatcgt tcctgcccgg gatgaggagc gtcgtgatct
      841 cggtccagac gtcggtctcg tgctcgaggt agacgagcgt ttcgaggacg ggagccagcc
      901 gcgcgcccgt gagcttcacg tagaactcgt cggtgaatcc cttgaggtcg acgttggccg
      961 cgtccatctt cgcgtagaac tcgcggcggg gctcgggcga gatgtagccc gcggtcaccg
     1021 ccaccgtgag gatgccggcc tcgcggcacg cgtcggccac gtccatcgcg tactcggcga
     1081 agatcaccgg atcgttgtag gtgaaggcga cgctcgcgca tcccgattcg gccgcggcga
     1141 tggcgatcgc ttccggagag gcctcgtcca tgagccggtc catgtcgcgc gatttcgaga
     1201 tgtcccagtt ctggcagaac ttgcacgcga ggttgcagcc cgccgtgcca aaggaaagaa
     1261 ccgcgctgcc gggcaggaaa tggttgaggg gcttcttttc gatgggatcg acgcagaatc
     1321 cggacgaccg tccgtaggtc gtgagcacca tcgcatcgcc cactcgcgcg cgcacgaagc
     1381 acgcgccgcg ctggccttcg tgcaggcggc aatcgcgcgg gcagaggtcg cactggacgc
     1441 gcccatcgtc gagccggtgc caccaccgcc ccggatgcag cgggccttcg atcgggcgcg
     1501 gcacggggtt caccggtact tctccaccgt gtagcgcgag agccggaggt cggggtgcca
     1561 gaagcgcgcc ggcagtcccg ccttccggcg caattcggcg aggaagtccg ccggctcggg
     1621 aagattctcc caaacctgcg gaagaaacgt cgcgctcatg tcgagatact gcaggtagat
     1681 gccgtcctcg ccgggacgca ggctcgcaag ggcctggtcc tcgctcgtag cggccagcgg
     1741 cacgcgcggc gtgagcaccg agacctcgat cgcgaccgag gcgcgttcgt ccggctgcaa
     1801 cggcggaaac cgcggatcgc gatatgcggc cgcgtgcgcg ttgcgcgcca cgtcgtctcc
     1861 cagcgggcg
//
LOCUS       DASVII010000383         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_89174, whole
            genome shotgun sequence.
ACCESSION   DASVII010000383 DASVII010000000
VERSION     DASVII010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89174"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF309_05030"
     CDS             <1..343
                     /locus_tag="VF309_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7465986.1"
                     /translation="VAFLFTHCGQLLYAPGTSEEKLMDAALEAGAQDVIAHDDGSLEA
                     VTTPHDFPAVKAALEKAGFKAELSEVTMKPVNETAMSGDDMARMQKLLDAIESLDDVQ
                     DVYTTASLGGA"
     gene            344..913
                     /locus_tag="VF309_05035"
     CDS             344..913
                     /locus_tag="VF309_05035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7465987.1"
                     /translation="MAIVTRALLLDIPEQIETPRLILAAARSGQGAMVNEAVVESAQE
                     LKPWMPWAQKPPTLEESEAYCREAQAKWAAREMLDFCFLRRSDQRLVGRGGLHTIDWS
                     IPKFEIGYWVRSSCARQGIATEATLALVELARDKLGARRLEITSDARNAPSRRVAEKS
                     GFTLEGILRRSRRDIAGELADSCMYARTF"
     gene            913..1437
                     /gene="ruvC"
                     /locus_tag="VF309_05040"
     CDS             913..1437
                     /gene="ruvC"
                     /locus_tag="VF309_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7465988.1"
                     /translation="MATIRILGIDPGLGCTGYGVLEMDRTTLVYVASGRIRSDEKLPL
                     AERLASILSGLAEVIDSYHPVEVAVEKVFVNVNPQSTLLLGQARGAAICAAVLARLPV
                     AEYTALQVKQSVVGQGHARKEQVQEMVKRLLKLPGHPSPDAADALACAICHAHGGLGA
                     LPARGKRVRAGRIS"
     gene            1434..2009
                     /gene="ruvA"
                     /locus_tag="VF309_05045"
     CDS             1434..2009
                     /gene="ruvA"
                     /locus_tag="VF309_05045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7465989.1"
                     /translation="MIGRILGKLVEKNPPQVVVVSHGVGYEIDVPMSTFYHLPRTGDD
                     VELLTHMVVREDAHLLFGFLTAAERTAFRQLLKISGVGPKVALSVLSGLSVEDLSIAV
                     ASQDAARLTKVPGVGKKTAERLVLELRDRLPAAIPTARSDASPASGDVVNALLGLGYN
                     EREAQGAMKQLPAGLPLADAIRHALKYLSKP"
     gene            2006..>2184
                     /locus_tag="VF309_05050"
     CDS             2006..>2184
                     /locus_tag="VF309_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7465990.1"
                     /translation="MIEADRLISGARTTPVEEGEERALRPKHLEEYVGQEKIREQLQI
                     FISAARNRKEALDHAL"
BASE COUNT          365 a          755 c          751 g          313 t
ORIGIN      
        1 cgtcgcgttc ctcttcaccc actgcgggca gctcctctac gcgccgggga ccagcgagga
       61 gaaactgatg gatgcggcgc tcgaagccgg cgcgcaggac gtgatcgcgc acgacgacgg
      121 ctccctcgag gcggtcacga cgccgcacga ttttcccgcg gtgaaggcgg ccctcgagaa
      181 ggcgggcttc aaggccgagc tctcggaagt gacgatgaag cccgtcaacg agaccgcgat
      241 gtcgggcgac gacatggcgc gcatgcagaa gctcctcgac gccatcgagt ccctcgacga
      301 cgtgcaggac gtctacacca cggcgtcgct gggcggagcc taagtggcga tcgtcacgcg
      361 ggcactcctc ctcgacattc ccgagcagat cgaaaccccg cggctgatcc tcgcggcggc
      421 gcgctcgggg cagggcgcca tggtgaacga ggcagtcgtg gaatccgcgc aggagctcaa
      481 gccctggatg ccgtgggcgc aaaagccgcc cacgctcgag gagagcgagg cctactgccg
      541 cgaagcgcag gcgaaatggg cggcgcggga aatgctcgat ttctgcttcc tgcgccgcag
      601 cgaccagcgc ctcgtgggcc gcggcgggct gcacaccatc gactggtcga tcccgaaatt
      661 cgagatcggc tactgggtcc gctcgtcgtg cgcgcgccag ggcatcgcca ccgaagccac
      721 gctcgcgctg gtggagctgg ctcgagacaa gctcggcgcg cgccgcctcg agatcacgtc
      781 ggacgcgcgc aatgccccga gccggcgcgt cgcggagaag agcggcttca ccctcgaagg
      841 catcctgcgc cggagccggc gcgacatcgc aggcgagctc gccgattcgt gcatgtacgc
      901 acgcacattc tgatggccac gatccggatt ctcggcatcg atcccggcct cggctgcacc
      961 gggtacggcg tgctcgagat ggatcgcacc accctcgtct acgtggcgag cgggaggatc
     1021 cgctccgacg agaagcttcc gctcgccgag cgcctcgcct cgatactctc cgggcttgcc
     1081 gaggtgatcg acagctatca tccggtcgag gtcgccgtcg agaaggtgtt cgtcaacgtg
     1141 aatccgcaat cgacgttgct tctcggccag gcgcgcggcg cggcgatctg cgccgcggtc
     1201 ctcgcccggc tgccggtcgc cgaatacacg gcgctccagg tgaagcagtc cgtggtgggg
     1261 cagggtcacg cgcgaaagga gcaggtccag gagatggtca agcggctgct gaagcttccc
     1321 ggacacccgt cgcccgacgc ggccgacgcg ctcgcgtgcg cgatctgcca cgcccatggc
     1381 gggctcggcg cgttgcccgc gcgcggcaag cgcgtgcgcg cgggtcgcat ctcgtgatcg
     1441 gccgcatcct cggcaagctc gtcgagaaga atccgccgca ggtcgtcgtg gtgagccacg
     1501 gcgtgggcta cgagatcgac gtgccgatga gcacgttcta tcacctgccg cgcacgggag
     1561 acgacgtgga gctcctcacc cacatggtgg tgcgcgagga tgcgcacctg ctcttcggat
     1621 tcctcacggc ggcggagcgc accgcgtttc gccagctcct caagatcagc ggcgtgggcc
     1681 cgaaggtcgc gctctcggtg ctctcgggac tgtcggtcga ggatctctcc atcgccgtcg
     1741 cctcccagga cgccgcgcgc ctcaccaagg tcccgggcgt cggcaagaag accgcggaga
     1801 ggctggtcct cgagctgcgc gaccgcctgc ccgccgcgat ccccacggcg aggagcgacg
     1861 cctcgcccgc gagcggcgac gtcgtcaacg cgctcctcgg ccttggctac aatgagcgcg
     1921 aggcgcaagg cgcgatgaag cagctccccg ccggcctgcc gttggccgac gccatacgcc
     1981 acgccctgaa gtacctgtcg aagccatgat cgaagccgac cgcctgatct cgggggcccg
     2041 gaccacgccg gtggaagaag gcgaggagcg ggcgctacgc cccaagcacc tcgaggagta
     2101 cgtggggcag gagaagatcc gcgagcagct gcagatcttc atctcggcgg cgcgcaaccg
     2161 caaggaagcg ttggaccacg cgct
//
LOCUS       DASVII010000384         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_233484,
            whole genome shotgun sequence.
ACCESSION   DASVII010000384 DASVII010000000
VERSION     DASVII010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233484"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="VF309_05055"
     CDS             <1..1661
                     /locus_tag="VF309_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7465991.1"
                     /translation="QTKKTVTAEWIRVGRNSSCEIHLPDPRIALEQGMIVNRSGLVYI
                     EGEAGSQDITRKSVRSVRMKPGEPLDIGPYRLEALPAPEGYDGAVAVELAHPLEIAPD
                     LRSRTERKTLASLGLSKRSAAWAGALAILVLYFLIPAARVLDLPWRQEAANAPIGDRA
                     WNPGHVILAHQPIENQCSACHEVAFQHVKNGACLECHARVASHVAPDMKPAALFAGTR
                     CTSCHTEHKGVKTTHRDDDKFCVDCHRDIGAKAPGAGSMNVADFAAAHPAFRLSIAEG
                     GATRRVRQDPRVPITQRSHLKFPHGVHVDAKGVKSPTKGRVRLECGGCHVPDASRRMF
                     EPISMAKQCQECHRLEIEPAVTARQAPHGKPADAVAMIEEFYATLALKGVPDSFQKAF
                     GVPGEGLLRRAGATEVERQDALRLATRKAQQVATDLFEVRVCKTCHEVTREPPADASA
                     PSWKIAPVRAASRWMPAARFDHRSHAQTKCADCHDVARSKSGDDVAMPGIESCRECHG
                     GSQPRSGKVTSNCLLCHHFHQSSHPWDPAFKPRAPERRAAKEAR"
     gene            1658..>2810
                     /locus_tag="VF309_05060"
     CDS             1658..>2810
                     /locus_tag="VF309_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465992.1"
                     /translation="MKAGLAAAIAFAATMAGCGGGSSGGSGAGGCASDCQGTQGFLTE
                     GDVQRVIAQAVGEAQARNVHAQIAIVDRVGNVLAVFQMPGAPATVAISSGLGVTGGLD
                     GIPAGTIPAAQAAIAKAITGAYLSSAGNAFSTRTAGQIIQEHFDPGESQQPSGPLYGV
                     QFSQLSCSDVNRNASQGSVGPKRSPLGLAADPGGLPLYKNGVVVGGIGVESDGRYGID
                     LEITDFDTAPEEIIAVAGASGFAAPADVRGDRITADGRTFHYVDSEATLSDPSHAPPF
                     ASLGGALGVVDGYNPGAIRAGTLFGSPDSGVRPDAGALASSGAWILVDAGNSARYAPR
                     DAADGTLRASDVQALLAEAIAIAKRARAQIRRPLGSSAEVSIAVVDRDGD"
BASE COUNT          445 a         1019 c          969 g          377 t
ORIGIN      
        1 cgcaaaccaa gaagaccgtc accgcggaat ggatccgcgt cgggcgcaac tccagctgcg
       61 agatccacct gccggacccg cgcatcgcgc tcgagcaggg aatgatcgtg aaccgcagcg
      121 gcctcgtcta catcgagggc gaggccggca gccaggacat cacgaggaag agcgtgcgct
      181 cggtgcgcat gaagcccggc gagccgctcg acatcggacc ctaccgcctc gaggcattgc
      241 ccgcgcccga aggctacgac ggagccgtcg ccgtcgagct cgcgcatccg ctggagatcg
      301 cgcccgacct gcgcagccgc accgagcgca agactctcgc atcgctcggg ttgtcgaaac
      361 gctccgccgc atgggcgggc gcgcttgcga tcctggtgct ctacttcctg attcccgcgg
      421 cccgcgtgct cgaccttccg tggcgccagg aggccgccaa cgcgccgatc ggcgaccgcg
      481 cgtggaaccc gggacacgtg atcctcgcgc accagccgat cgagaaccaa tgctcggcat
      541 gccacgaggt cgcgttccag cacgtgaaga acggcgcctg cctcgagtgc catgcgaggg
      601 tcgcaagcca tgtcgcgccc gacatgaaac ccgccgctct cttcgccgga acgcgctgca
      661 ccagctgcca cacggagcac aagggcgtga agaccacgca ccgcgacgac gacaagttct
      721 gcgtcgattg ccatcgcgac atcggcgcca aggcgcccgg cgccggctcg atgaacgtcg
      781 ccgatttcgc ggctgcgcat cccgcgtttc gcctctcaat cgcggagggc ggcgcgacac
      841 ggcgcgtgcg ccaggacccg cgcgtcccga tcacgcagcg ctcccacctg aagttccccc
      901 atggcgttca cgtcgatgcg aagggcgtga agtcgccgac caaggggcgc gtgaggctcg
      961 aatgcggggg ctgccacgtg cccgacgcct cgcgccgcat gttcgagccg atctcgatgg
     1021 cgaagcaatg ccaggagtgc caccgcctcg agatcgagcc cgccgtcacc gcccgccagg
     1081 cgccccatgg aaagcccgcc gacgccgtcg cgatgatcga ggagttctac gcgacgctcg
     1141 cattgaaggg cgtccccgac agcttccaga aggcgttcgg cgtgccgggc gagggactgc
     1201 tgcggcgcgc gggcgcgacg gaagtggagc gccaggacgc cttgcgcctc gcgacgcgca
     1261 aggcgcagca ggtcgcgacc gacctcttcg aggtgcgcgt ctgcaagaca tgccacgagg
     1321 tgacgcgcga gccgccggcg gacgcgtcgg cgccttcctg gaagatcgcg ccggtgcgcg
     1381 cggcgagccg ctggatgccc gcggcgcgct tcgaccaccg aagccacgcg cagacgaagt
     1441 gcgccgattg ccacgacgtg gcgcgctcca agagcggcga cgacgtcgcg atgcccggga
     1501 tcgagtcctg ccgcgagtgc cacggcggct cgcagccgcg gtcgggcaag gtgacgtcga
     1561 attgcctcct atgccaccac ttccaccaat cgagccatcc gtgggatccc gccttcaagc
     1621 cgcgcgcacc cgagcgccgc gccgcgaagg aggcgcgatg aaggcggggc tcgccgcggc
     1681 gatcgccttc gcggcaacga tggcgggctg cggcggcggc tcgagtggcg gcagtggcgc
     1741 cggcggatgc gcgagcgatt gccagggcac gcagggcttc ctcaccgaag gcgacgtgca
     1801 gcgcgtgatc gcgcaagccg tgggcgaagc gcaggcgcgc aacgttcacg cgcagatcgc
     1861 gatcgtcgat cgcgtcggca acgtcctcgc cgtattccag atgccgggcg cgcccgcgac
     1921 ggtcgcgatc tcgagcgggc tcggcgtcac gggcgggctc gacggcattc cggccggcac
     1981 cattccggcg gcgcaggcgg ccatcgccaa ggcgatcacg ggggcttacc tctcctcggc
     2041 gggcaacgcc ttctcgacgc gcaccgcggg acagatcatc caggagcatt tcgatcccgg
     2101 cgagtcgcag caaccgtcgg gtccgctgta tggcgtgcag ttctcgcagc tttcctgctc
     2161 cgacgtgaat cgcaacgcct cgcagggcag cgtcggcccg aagcgctcgc cgctcggcct
     2221 cgccgcggat ccgggaggcc tgccgctcta caagaacggc gtcgtcgtgg gcggaatcgg
     2281 cgtcgagtcc gacgggcgct acgggatcga tctcgagatc accgacttcg acaccgctcc
     2341 cgaggagatc atcgcggtgg ccggcgccag cggcttcgcc gcgccggccg acgtgcgcgg
     2401 cgatcgcatc accgccgacg gtcgaacctt ccactacgtc gattccgagg cgacattgag
     2461 cgatccgtcg cacgcgccgc ccttcgcgtc gctcggcggc gcgctcgggg tcgtcgatgg
     2521 atacaacccc ggggcgattc gcgcgggaac gctcttcggc tctcccgact cgggcgtgcg
     2581 tcccgatgcc ggagcgctcg cgtcgtccgg cgcgtggatc ctcgtcgacg cgggaaattc
     2641 cgcgcgctac gcgcctcgcg acgccgccga cggcacgctt cgcgcaagcg acgtgcaggc
     2701 gctcctcgcc gaagcgatcg ccatcgcgaa gcgcgcgcgc gcgcagatcc ggcgcccgct
     2761 cggctcgagc gccgaagtgt cgatcgcggt ggtcgatcgc gacggcgaca
//
LOCUS       DASVII010000385         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_593072,
            whole genome shotgun sequence.
ACCESSION   DASVII010000385 DASVII010000000
VERSION     DASVII010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593072"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VF309_05065"
     CDS             complement(<1..1294)
                     /locus_tag="VF309_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7465993.1"
                     /translation="MGKARAEEGSALVVATLLVVACIGLVFAIGLASPASSARADRAS
                     ERALAEAREALIAYAADRPINAVVGPGYLPCPDLDDDGWAESTCGSQAGDSGQAQRLG
                     RLPWKTLGISDLRDGYGERLWYAVSSKYKGLLNCGVSRACLDMTPGTALGTITVRDNT
                     GAIVHDGTVADAYRADAGGAVAIVIAPGHALERAGGAAQSRGCSPQDCDASGRCVTDP
                     PQRADHCNPVNYLDKASGARYADEDNADFVDRNDGAGRPRNANGFIQGPVAFPDGRVA
                     VNDRIAVVAYRDVMPRIMARVALEASHCLRRYASSPENRGRYPWATPACLQSGDVWEG
                     RRGARFGRIPDTPFADAADTAMLDRWWRWQSRAPENLLELPTANDACRIAIAPGDDGA
                     HRSLPAGNPAGEGGTAGHDANSWWSAWKPYVFYALASGF"
BASE COUNT          204 a          544 c          555 g          225 t
ORIGIN      
        1 agaagccgct cgcgagcgca tagaagacgt agggcttcca ggcgctccac cacgagttcg
       61 catcgtggcc cgcggtaccg ccttcgcccg cgggattgcc cgccggcagg ctccgatgcg
      121 cgccatcgtc gccgggcgcg atcgcgatgc ggcacgcatc gttcgcggtc gggagctcga
      181 gaagattttc cggagcgcgc gattgccagc gccaccagcg atcgagcatc gccgtgtccg
      241 cggcatcggc gaagggcgtg tcgggaatcc ggccgaatcg cgcgccgcgg cgcccttccc
      301 acacgtcccc ggattgcagg cacgcgggcg tcgcccacgg gtagcggccc cgattctcgg
      361 ggctcgacgc atagcgccgc aggcagtggc tcgcctcgag cgcgaccctc gccatgatgc
      421 gcggcatcac gtcgcgatag gccacgacgg cgatgcggtc gttcaccgcg acgcgtccgt
      481 cgggaaacgc gaccgggccc tggatgaatc cattggcgtt gcgcggccgc ccggccccgt
      541 cgttcctgtc gacgaagtcg gcgttgtcct cgtccgcata gcgcgcgccg gaggccttgt
      601 cgaggtagtt caccggattg cagtgatccg cgcgctgcgg cggatcggtg acgcagcgtc
      661 ccgacgcgtc gcagtcctgc ggactgcacc cgcgtgattg cgcggcgccg cccgcgcgct
      721 cgagcgcgtg gcccggtgcg atcacgatcg cgacggcgcc gcccgcgtcg gcccgatacg
      781 cgtcggcgac ggtgccgtcg tggacgatag cgcccgtgtt gtcgcgcacc gtgatcgtgc
      841 cgagcgcggt cccgggagtc atgtcgaggc acgcgcggct tacgccgcag ttcagcaggc
      901 ccttgtactt gctcgacacc gcgtaccaga gccgctcgcc gtagccgtcg cgcaggtcgg
      961 agatgccgag ggtcttccac ggcaggcggc cgaggcgctg cgcctggccg ctgtcgcccg
     1021 cctgcgatcc gcacgtcgat tcggcccagc cgtcgtcgtc gaggtccggg cacggaaggt
     1081 agccggggcc cacgaccgcg ttgatcgggc gatcggccgc gtaggcgatg agcgcctcgc
     1141 gcgcctcggc gagggcgcgt tcgctcgccc ggtcggcgcg cgcgctcgat gcgggagagg
     1201 cgagcccgat cgcgaacacg aggccgatgc acgcgacgac gagaagcgtg gcgaccacga
     1261 gcgcgctgcc ttcctctgcg cgcgccttcc ccatgtcccg cctccgcttt cgaatgtccc
     1321 ggctactgga tagcagcccg gcgcccggcg ggcgcacgaa aaagctccaa tgcgattcgc
     1381 ggacttcgct tgactagggc ggcggcttgc gctcgatgcg aagccggtct gcgccggggg
     1441 gcgcgccgag gatcgccggg tcgaggaggg gatcggcgcc ggagcgccgc acgacgaccg
     1501 gggtgccgtc gatccatatc gtgttgcg
//
LOCUS       DASVII010000386         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_665038,
            whole genome shotgun sequence.
ACCESSION   DASVII010000386 DASVII010000000
VERSION     DASVII010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665038"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..2013
                     /locus_tag="VF309_05070"
     CDS             178..2013
                     /locus_tag="VF309_05070"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX7465994.1"
                     /translation="MTGKGASRAGQLGAWVDGWSALLGPERVHWCDGSREEFDALTAA
                     MVEQGTLLRLDPRKRPRSFLCRSDPRDVARVESRTFICSRVRGDAGPTNNWVAPAAMQ
                     ERLRASFTGAMRGRCLYVIPFSMGPIGSPIAQIGVQLSDSPYVAASMRIMTRMGSAVL
                     DVLGESDDFVRCVHSVGAPLAPGKADVPWPCNPPETTIAHFPEERTIWSYGSGYGGNA
                     LLGKKCFALRIASVMGRDEGWLAEHMAILGLQSPTGERTYVAAAFPSACGKTNFAMMV
                     PPAGFEGWKLWTVGDDIAWIMPGRDGRLHAINPEAGFFGVAPGTNAESNPNAMQTIAH
                     DTIFTNVALTDDGDVWWEGMTREPPAHLVDWQGKDWTPGCGRVAAHPNSRFTAPLQNC
                     PSLDARWDDPRGVPVSGFIFGGRMSQDMPLVFQSFNWSHGVYSAATMASEATAAAEGL
                     PPLRRDPMAMLPFCGYNMADYFSHWLNVGRRIPNPPRIFRVNWFLRDKDGKFLWPGFS
                     QNTRILAWIVDRIMGRGFAVESPLGWMPRYEDVHWSGLDFPRERFHDLMTVSRDAAAA
                     EVRSHEELFDRFLDRVPKEFLFERELLRSRLWRSPEQWELADEVV"
BASE COUNT          292 a          705 c          688 g          330 t
ORIGIN      
        1 gctcgcccgg gccgggcgga cgcccgtcgt caggggggcc ttcggccgaa ggcggcgaca
       61 tcgcgcgcaa tctaccatgc ttcgtcttcc tgctcgtcgg tcgctctcgg gccccgcgat
      121 ccgtttcaga acggatctgc catcccgcgc gcacccgatg ccgtagattt gaggatcatg
      181 accggaaaag gtgcgagtcg cgcggggcag ctcggggcgt gggtcgacgg gtggtccgcc
      241 ctgctcggcc cggagcgagt ccactggtgc gacggcagcc gcgaagagtt cgacgccctc
      301 accgccgcga tggtcgagca gggcaccctg ctgcgcctcg acccccggaa gcggccgcgc
      361 agcttcctgt gccgctccga tccgcgcgac gtggcgcgcg tcgagagccg cacgttcatc
      421 tgttcgcggg tgcgcggcga cgccgggccc accaacaact gggtcgcgcc ggccgccatg
      481 caggagcgcc tgcgcgcctc cttcaccggc gcgatgcgcg gccggtgcct ctacgtgatc
      541 ccgttcagca tgggccccat cggatcgccc atcgcgcaga tcggcgtgca gctctccgat
      601 tcgccttacg tcgccgcgag catgcgcatc atgacccgca tgggcagcgc ggtgctcgac
      661 gtgctcggcg agagcgacga cttcgttcgt tgcgtgcact cggtcggcgc ccccctcgcg
      721 ccggggaagg ccgatgtgcc gtggccctgc aatccgcccg agaccaccat cgcccacttt
      781 cccgaggagc ggacgatctg gtcatatggc agcggctatg gcggcaacgc gctcctcggc
      841 aagaagtgct tcgcgctacg gatcgcgtcg gtgatgggcc gcgacgaggg gtggctggcg
      901 gagcacatgg ccatcctcgg cctccagtct cccaccggcg agcgcaccta tgtcgccgcc
      961 gcgttcccga gcgcatgcgg caagaccaac ttcgcgatga tggtgccgcc cgccggtttc
     1021 gagggctgga agctgtggac ggtcggcgac gacatcgcct ggatcatgcc aggccgtgat
     1081 gggcgcctgc acgccatcaa ccccgaggcc ggtttcttcg gggtggcgcc gggcaccaac
     1141 gccgagagca acccgaacgc catgcagacc atcgcccacg acacgatctt caccaacgtc
     1201 gccctgaccg acgacggcga cgtgtggtgg gagggcatga cccgcgagcc gcccgcgcac
     1261 ctcgtcgact ggcagggcaa ggactggacg cccggttgcg gccgggtcgc cgcgcaccca
     1321 aactcgcgct tcaccgcgcc cttgcagaat tgtccctcgc tcgacgcacg ctgggacgac
     1381 ccgcgcggcg tgccggtgag cggcttcatc ttcggcgggc ggatgagcca ggacatgccg
     1441 ctcgtgttcc agtcgttcaa ctggtcgcac ggcgtgtaca gcgccgcgac catggcgtcg
     1501 gaggccacgg cggcggccga aggcctgccg ccactgcggc gtgacccgat ggccatgctg
     1561 ccgttttgcg gctacaacat ggccgattat ttcagtcact ggttgaacgt ggggcgccgc
     1621 atcccgaacc cgccgcgcat ctttcgtgtc aactggttcc tgcgcgacaa ggacggcaag
     1681 ttcctgtggc ctgggttcag ccaaaatacc cgcatcctgg cctggatcgt cgatcgaatc
     1741 atggggcgcg gctttgcggt ggagagcccg ctcggctgga tgccgcgcta cgaggatgtg
     1801 cactggagcg gcctcgactt cccgcgcgag cgctttcacg atctgatgac cgtgagccgt
     1861 gacgccgccg ccgccgaggt gcgctcgcac gaggagctgt tcgaccgctt cctcgaccgc
     1921 gtgcccaagg agtttctgtt cgagcgcgag ctgctgcgtt cgcgcctgtg gcgctcgccc
     1981 gagcagtggg agctggcgga cgaagtggtg tgagc
//
LOCUS       DASVII010000387         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_341674,
            whole genome shotgun sequence.
ACCESSION   DASVII010000387 DASVII010000000
VERSION     DASVII010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341674"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VF309_05075"
     CDS             <1..974
                     /locus_tag="VF309_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX7465995.1"
                     /translation="RTPLPWMIGSLVTMAALRMLGMRLEALPAGRSVGQLVVGVSLGL
                     YFTAPVVHEVASFWPWFVFLGFAAIGLGAASALVLLSLGKVDRATAYFSSMPGGAADM
                     ATMGERHGAAMDLVAFAHSARMLLVVTVIPVSITLAGFSATDEYRPVTIAFDAAGLAA
                     LFALAAASAWMAQKLGAPTAFTMGPLFLSIALTASDISFSSVPTPLTNAAQVLLGCSL
                     GSRFDRRFLAGAPRFVAALVPSIGMMLLLAALVGWLIAFTSGVYLGTALLAAAPGGIA
                     EMAITAKVLRIGVAFVTAAHVVRYVIVVLFTIPMFHVLEGARRRVRR"
     gene            987..>1739
                     /locus_tag="VF309_05080"
     CDS             987..>1739
                     /locus_tag="VF309_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7465996.1"
                     /translation="MRNLLEKAAPMTTLKLSLMSFVFLAVQAFAVSAQPYPSKPIRII
                     VPYPPGGTSDILARSVGQKLTETLGQSVVVENKSGATGNIGAEFVAKSPPDGYTLLLA
                     DIGSLAIAPSVTASLPFDPVKDFAPVVMVAYSPHILALNPAVPANNVRELVALLKAKP
                     DSLNFAVSGVGGANHLAGIDFAARTGVKWTYIPYKGGSQAIADLMAGHAQVMFNGMLA
                     TYPAVKEGKLKAIAISSAKRFAVAPDLPTVAES"
BASE COUNT          234 a          611 c          578 g          316 t
ORIGIN      
        1 tgcgcacgcc gctgccgtgg atgatcggct cgctcgtgac catggcggcg ctgcgcatgc
       61 tgggcatgcg cctcgaggcg ctgcccgccg gacgaagcgt cgggcagctc gtggtgggcg
      121 tgagtctcgg gctctatttc accgcgcccg tggtgcacga ggtcgcgagc ttctggccgt
      181 ggttcgtgtt cctcggattc gcggcgatcg ggctcggcgc ggccagcgcg ctggtgctcc
      241 tctcgctggg aaaggtcgat cgcgcgaccg cgtacttcag cagcatgccg ggaggcgccg
      301 cggacatggc gacgatggga gagcgccacg gcgcggcgat ggatctcgtc gcgttcgcgc
      361 actcggcgcg catgctgctc gtggtgacgg tgatcccggt gtcgatcacg ctcgcgggat
      421 tctccgccac cgacgagtac cggccggtca ccattgcctt cgacgcggcg gggctcgccg
      481 cgctcttcgc gctcgcggcc gcttccgcat ggatggcgca gaaactcggc gctcccaccg
      541 cgttcaccat gggtccgctg tttctcagca tcgcgctcac ggcgtctgac atcagcttct
      601 cgtcggtgcc gacgccgctc acgaacgcgg cacaggtgct gctcgggtgc tcgctcggat
      661 cgcgcttcga ccggcgcttc ctcgcgggcg cgccgcgctt cgtcgcagcg ttggttcctt
      721 ccatcggcat gatgctgctg ctcgccgcat tggtgggatg gctcatcgcg ttcaccagcg
      781 gcgtctacct cggcaccgcg ctcctcgcgg cggctccggg cggcatcgcg gaaatggcga
      841 tcacggcgaa ggtgctgcgc atcggcgtcg ccttcgtcac ggccgcgcac gtggtgcgct
      901 acgtgatcgt ggtcctcttc accatcccga tgttccacgt gctcgagggc gcgcgccgca
      961 gggtccggcg ctgacttcga gcgagaatgc gcaatctact cgagaaggcc gcccccatga
     1021 cgactttgaa gctctccctc atgtctttcg tatttctcgc cgttcaggct ttcgcggttt
     1081 ccgcgcagcc ctacccgtcg aagcccatca ggatcatcgt gccgtatccg ccgggcggca
     1141 cctccgacat cctcgcgcgc tccgtgggac agaagctcac cgagaccctc gggcagtcgg
     1201 tcgtggtcga gaacaagtcc ggcgcgaccg gcaacatcgg cgcggagttc gtcgccaagt
     1261 cgccgcccga cggctacacg ctgctgctcg ccgacatcgg ctcgctcgcc atcgcgccga
     1321 gcgtgaccgc gtcgctgccg ttcgatccgg tgaaggactt cgcgccggtc gtgatggtcg
     1381 cgtactcgcc gcacatcctc gcgctcaatc ccgcggtgcc cgccaacaac gtgagggagc
     1441 tggtggcgct cctcaaggcg aaacccgaca gcctcaactt cgcggtctcc ggcgtcggcg
     1501 gcgcgaacca tctcgccggc atcgatttcg ccgcgcgcac cggcgtgaag tggacctaca
     1561 ttccttacaa gggcggctcg caggcgatcg ccgacctcat ggcgggacac gcgcaggtca
     1621 tgttcaacgg catgctcgcg acctacccgg cggtgaagga aggcaagctg aaggcgatcg
     1681 cgatctcgag cgcgaagcgt ttcgccgtgg cgccggacct gccgaccgtc gcggagtcg
//
LOCUS       DASVII010000388         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_449129,
            whole genome shotgun sequence.
ACCESSION   DASVII010000388 DASVII010000000
VERSION     DASVII010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449129"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VF309_05085"
     CDS             complement(<1..1161)
                     /locus_tag="VF309_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7465997.1"
                     /translation="MLTGRTAVAGEETPSARAERARAEGTKALAAGDFDRARRQFEEV
                     LAILPGDASAARDAARAAEAAQQFEYAAEVLERAHHFDEHRPDPELHYLRGEALYALN
                     RIEEARLEHRIAELEIDLRPADRMAKLWLARIYARRGELARADAVYESLWPPAPAFDA
                     EAATNQAEAHVLNRDWPGGQRVIERLLARDPKNLRARELLAYILEVRGNLDRELPVRQ
                     EVAVDQPTADRLLGWGRALERADDFRGAYDRYEDARAMSNGEANETLATSLLKMHYRI
                     TPEAGGGFVGRRDPQADGLRAQAGLALPFGARHSLTFLGWNETTRGRVLRNNVLVPGG
                     GSASGVSSTLTLADRRGLSLSLGGELRSVSPASAGMEPGFAPEHTEAGAATEV"
BASE COUNT          229 a          561 c          487 g          239 t
ORIGIN      
        1 cacctcggtc gccgctccgg cctccgtgtg ttccggcgcg aaaccgggct ccattccggc
       61 agacgcgggg gagaccgagc ggagctcgcc ccccaatgac agcgacaacc ctcgccgatc
      121 cgcgagggtc agcgtgctcg agacgccgga cgcactaccg cctccgggca cgagcacgtt
      181 gtttcgcaac accctgcccc gtgtcgtctc attccagccg aggaatgtca gactgtgccg
      241 ggccccgaac ggcagggcaa ggccggcctg ggcacgcaag ccgtcggcct gaggatcgcg
      301 gcgaccgacg aaaccaccgc ccgcttcagg cgtgatccgg tagtgcatct tcagcagcga
      361 cgtggcgagg gtttcgttcg cctccccgtt cgacatcgct cgcgcgtctt cgtagcggtc
      421 ataggcgccg cggaaatcat cggcgcgctc gagcgcgcgc ccccacccga gcaacctgtc
      481 ggcggtgggc tggtcgaccg ccacctcctg cctcacgggc aactcccgat cgagattacc
      541 ccgcacctcg aggatgtacg ccagcagctc gcgcgcgcgg aggttcttgg gatcgcgggc
      601 gagcagccgt tcgatcacgc gctgcccccc tggccagtcc cgattgagca cgtgcgcctc
      661 cgcctgattg gtcgccgcct cggcgtcgaa cgccggcgcg ggcggccaca gcgattcgta
      721 gacggcgtcg gcgcgcgcga gctcgccgcg gcgggcgtag atccgcgcca accagagctt
      781 cgccatgcga tccgccggac gcaggtcgat ctccagctcc gcgatccggt gctcgagccg
      841 cgcctcctcg attcgattga gggcgtagag ggcctccccg cgcaagtagt gcagctcggg
      901 atcggggcga tgctcgtcga aatggtgcgc cctttcgagc acctccgcgg cgtattcgaa
      961 ctgctgcgcg gcttccgccg ctcgtgccgc gtcccgcgcc gcgctggcgt cgcccggcag
     1021 gatggcgagc acctcttcga actgccggcg cgcgcggtcg aaatcgcccg ccgccaacgc
     1081 cttcgtcccc tccgcgcgtg cgcgctcggc gcgcgcggag ggggtctcct cgccggcgac
     1141 ggccgtgcgc cccgtgagca ccactgccga aagcgcacaa gcgatagtaa cgctcggaaa
     1201 gccacgactc ctcatcaccc acttcctttc gcgctcatgt tcgcgatgcg ggactgcaga
     1261 aaccgtgccc gggaatctgg ctgtcttcgg gcgcgccgcg cagctgatca cgcaattcgt
     1321 acgccactcc cggtcgtctg cgaaaaccta gcgtgacggc gccttttgcc gccgcgcctc
     1381 gtcaggagac aagtcctttc gtctgcgcgc tgaccaagcg ccgtcggaat ttctcccccg
     1441 tggcacgtcg gatgcttcag gccgaaccaa cgcgccgcct ttcaggaaga cggttcgcga
     1501 aggagaggaa cggcaa
//
LOCUS       DASVII010000389         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_701142,
            whole genome shotgun sequence.
ACCESSION   DASVII010000389 DASVII010000000
VERSION     DASVII010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701142"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1541
                     /gene="dnaE"
                     /locus_tag="VF309_05090"
     CDS             <1..>1541
                     /gene="dnaE"
                     /locus_tag="VF309_05090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7465998.1"
                     /translation="LLDGAIRLEDLFPKVLERGMKSVAITDHGNLFGALDFYETAKKY
                     GVKPIFGCETYIAPDRLDKTERRSNHLILLAKDNVGWKNLSYLNSMGYLEGFYYNPRI
                     DKKLLREHSEGLIGLSACLGGEVAQTCMRRGTEAALAVAREFDDIFGRGNFFLEVQPN
                     GLEEQEKVNGELYAMAKKTGIPLIATNDCHYLNKQDARAHEILMCVQQKKTIHDEKRL
                     HHRNEAFFVKTPAEMDAYFAHVPEAMENAARIGELCNVTFDLGHTYLPKFKVPEGFDA
                     DSYVTHLAEEGLKKRLEEAYRRGRKFNGDAYAARLALELGVIKKMEFSGYFLIVWDFI
                     RYAREHGIPVGPGRGSGAGSLVAFCLRITDIDPLEHKLLFERFLNPERVSMPDFDIDF
                     CMNRRDQVITYVTEKYGKNNVGQIITMHQIKARSGVRDIARAMALPFAEADKVAKLVP
                     EPIQGKSPPIAEAIEQEPRLKALYEENGVYRELLDVAKSLEGLNRHAGKHAAGIVIAE
                     RPLWE"
BASE COUNT          311 a          489 c          509 g          232 t
ORIGIN      
        1 gcctcctcga tggcgcgatt cggctagagg acttattccc gaaggttctg gagcggggca
       61 tgaaatcggt ggccatcacc gatcacggga acctgttcgg cgcgctcgat ttttacgaga
      121 ccgcgaagaa atacggcgtg aagccgatct tcggctgcga gacctatatc gcgcccgacc
      181 gcctcgacaa gaccgagcgg cgcagcaacc acctcatcct gctcgccaag gacaacgtcg
      241 gctggaagaa cctgtcgtac ctcaactcga tgggttatct ggaaggcttc tattacaacc
      301 cccgcatcga caagaagctg ttgcgcgagc acagcgaggg gctgatcggc ctgtcggcgt
      361 gcctgggcgg cgaggtcgcg cagacgtgca tgcgccgcgg gaccgaggcc gcccttgcgg
      421 tggcgcgcga gttcgacgac atcttcgggc gcggcaactt cttcctcgag gtacagccga
      481 acggcctgga ggagcaagag aaggtgaacg gcgagctcta cgcgatggcg aagaagaccg
      541 gcatcccgtt gatcgcgacg aacgactgcc actacctgaa caagcaggac gcgcgcgcgc
      601 acgagatcct gatgtgcgtg cagcagaaga agaccattca cgacgaaaaa cgcctccacc
      661 accgcaacga ggcgttcttc gtgaagacgc cggccgagat ggacgcgtac ttcgcccacg
      721 tgcccgaggc gatggagaac gccgcgcgca tcggcgagct gtgcaacgtc acgttcgatc
      781 tgggccacac gtacctgccc aagttcaagg tcccggaggg cttcgacgcc gacagctacg
      841 tcacgcacct cgccgaggag ggcctgaaga agcggctcga ggaggcctac cgccgcgggc
      901 ggaagttcaa cggcgacgcg tacgccgcgc gcctcgcgct cgagctcggc gtgatcaaga
      961 agatggagtt ctcgggctac ttcctgatcg tgtgggactt catccgctac gcgcgcgagc
     1021 acggcatccc ggtgggtccg gggcgcggct cgggcgccgg gtcgctggtg gcgttctgcc
     1081 tgcgcatcac cgacatcgat cccctcgagc acaagctgct gttcgagcgc ttcctgaacc
     1141 ccgagcgcgt cagcatgccg gacttcgaca tcgacttctg catgaaccgg cgcgatcagg
     1201 tgatcaccta cgtcaccgag aagtacggca agaacaacgt cgggcagatc atcacgatgc
     1261 accagatcaa ggcgcgcagc ggggtgcgcg acatcgcgcg cgcgatggcg ctgccgttcg
     1321 ccgaggccga caaggtcgcg aagctggtgc ccgagccgat ccagggcaag tcgccgccca
     1381 tcgcagaagc gattgaacaa gagccgcggc tgaaggcgct gtacgaggag aacggcgtct
     1441 accgcgagct gctggacgtc gcgaagtcgc tggagggcct gaaccgccac gcgggcaagc
     1501 acgccgccgg gatcgtcatc gccgagaggc cgctgtggga g
//
LOCUS       DASVII010000390         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_269748,
            whole genome shotgun sequence.
ACCESSION   DASVII010000390 DASVII010000000
VERSION     DASVII010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269748"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VF309_05095"
     CDS             complement(<1..235)
                     /locus_tag="VF309_05095"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEX7465999.1"
                     /translation="MDGIHDLGGRQGFGKIRYTLGATAFHAAWEVRANSLYAHAVRLR
                     IFNMDEYRHAIERMDPRHYLTASYYERSLTSLAT"
     gene            complement(238..843)
                     /gene="nthA"
                     /locus_tag="VF309_05100"
     CDS             complement(238..843)
                     /gene="nthA"
                     /locus_tag="VF309_05100"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX7466000.1"
                     /translation="MPDTDRVIQRTASVEERVDAIQAALDERGLKASEAVEELGHLAQ
                     ETWIPENGARVVARAWVDPDFRKRLLANGREAVAELGLAMPKHHRHLVVLENTPQVQN
                     VICCTLCSCTAFTIIGLPPDWYKDLEYRSRVVRESRTVLREMGLDLPPQVEIRVWDTT
                     ADTRYMVLPEQPPGTEGWSEDKLAALVTRDSMIGVARLGAP"
     gene            complement(846..>1718)
                     /locus_tag="VF309_05105"
     CDS             complement(846..>1718)
                     /locus_tag="VF309_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HEX7466001.1"
                     /translation="FEVNGALLSRAQVYVLESLKPEDLDLLIQRGFEAEKVDSTADAR
                     ARIAEFADGDGRRALNLVEQVAQAARLEGRHGVDVEFVNAIAQRAGRRFDKGGENFYD
                     QISALHKSVRGSDPDASLYWLVRMLDGGVDPRYLARRMIRMASEDIGLADPRALEVAI
                     TAADVYERLGTPEGELALAECVIYLAIAAKSNAVYTAYGAVRAFIAEDGTRPVPLHIR
                     NAPTKLMKNLGYGKDYRYAHGEEEGYAAGETYLPDGMEPQHWYQPTDRGLEAKIREKL
                     EHLRELDLKAARKK"
BASE COUNT          245 a          589 c          580 g          304 t
ORIGIN      
        1 gcgtcgcgag gctcgtgagc gagcgctcgt agtagctcgc ggtcaggtaa tggcgcgggt
       61 ccatgcgctc gatcgcgtgg cgatattcgt ccatgttgaa gatgcgcagc cgcaccgcat
      121 gggcgtagag cgagttggca cgaacctccc aggccgcgtg gaacgcggtc gcgcccagcg
      181 tgtaacgtat cttgccgaag ccctgccgcc cgccgaggtc gtggatgccg tccatggtca
      241 gggcgcgccg aggcgtgcga cgccgatcat cgaatcgcgg gtcacgaggg ccgcgagctt
      301 gtcttcgctc cagccctcgg tccccggggg ctgctcggga agcaccatgt agcgagtgtc
      361 cgcagttgtg tcccacacgc ggatctcgac ttgcggcggc aggtccagtc ccatctcgcg
      421 caatacggtc cgcgattcac gcacgacgcg cgagcggtat tcgagatcct tgtaccagtc
      481 gggcggcagg ccaatgatcg tgaacgcggt gcacgagcac aaagtgcagc agatgacgtt
      541 ctgcacctgc ggcgtgttct ccagcaccac aaggtggcga tgatgcttgg gcatcgcgag
      601 gcccagctcg gccacggcct cgcgcccgtt ggcgagcagg cgcttgcgga agtccgggtc
      661 gacccatgcc cgcgcgacga cgcgcgcgcc attctccggg atccacgttt cctgggcgag
      721 gtggcccagc tcttccacgg cttcggacgc cttgaggccg cgctcgtcga gcgccgcctg
      781 gatcgcgtcg acgcgctctt ccacgctcgc cgtgcgctgg atgactcggt cggtatcggg
      841 catggtcact tctttctcgc cgctttcagg tccagctcgc gcagatgctc gagcttctcc
      901 cggatcttcg cttccagccc gcggtcggtg ggctggtacc agtgctgcgg ctccattccg
      961 tcgggcaggt atgtctctcc cgcggcgtag ccctcctcct cgccgtgcgc gtagcgatag
     1021 tccttgccgt agcccaggtt cttcatgagc ttcgtcggcg cgttgcggat gtgcagcggc
     1081 acgggccgcg tgccgtcctc ggcgatgaag gctcgcaccg cgccatatgc agtgtagacg
     1141 gcgttggact tcgcggcgat cgcgaggtag atcacgcact ccgcgagggc gagctcgcct
     1201 tcgggagtcc cgaggcgctc gtagacgtcg gccgcggtga tggccacctc gagcgctcgc
     1261 gggtccgcga gcccgatgtc ctcgctcgcc atgcggatca tgcgacgcgc gaggtagcgc
     1321 ggatcgacgc cgccgtcgag catgcgcacc agccagtaga gggacgcgtc gggatcgctc
     1381 ccgcgcacgc tcttgtgcag cgccgagatc tggtcgtaga agttctcgcc gcccttgtcg
     1441 aagcgccgcc cggcgcgctg ggcgatggcg ttcacgaact ccacgtccac gccgtggcgc
     1501 ccctccaggc gcgcagcctg ggccacctgc tcgaccaggt tcaacgcgcg ccgcccgtcg
     1561 ccatcggcga actcggcaat gcgcgcacgg gcatccgcgg tggaatcgac cttctcggcc
     1621 tcgaagccgc gctggatgag gaggtcgaga tcctcgggct tgaggctctc cagcacgtac
     1681 acctgcgcgc gcgagagcag cgcgccgttc acctcgaa
//
LOCUS       DASVII010000391         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_665334,
            whole genome shotgun sequence.
ACCESSION   DASVII010000391 DASVII010000000
VERSION     DASVII010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665334"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF309_05110"
     CDS             <1..663
                     /locus_tag="VF309_05110"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX7466002.1"
                     /translation="WQLKRRWFWQQSHNDVIQKVLRKARKGTRVTYIAGNHDEAMRHF
                     LGLSFGGIDIRDEATHVTASGKRLLVIHGDLFDAVVQGAKWLAHAGDWAYMMSLKLNR
                     WFNHARARVGLPYWSLAQFLKHRVKNAVSYIGNFEDALAHEARRRDFDGVVCGHIHKA
                     EIRDIGGVLYCNDGDWVESLTALVELDNGELRIIDWKAIEALRRDAVAFEGAEIAHSP
                     GD"
     gene            656..1669
                     /locus_tag="VF309_05115"
     CDS             656..1669
                     /locus_tag="VF309_05115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7466003.1"
                     /translation="MTDAWAPQINGVVVTLRNTVAWLERWGHEVHVLSPEGFKTFPMP
                     TYPEIPLAIFPAREVNRRIAALAPDAIHIATEGPLGIAARAYCVKHDLSFTTAYHTCF
                     PEYVKPRFGIPLAWTYAWLRRFHGRSSALLVATPAIQALLEARGFSNIAEWSRGVDLE
                     LFKPAEHRFSDLRRPVFLYVGRVAVEKNLPAFLALDLPGTKLVVGDGPQRKELEERFP
                     DAVFVGAKTGPELASYYQRADVFVFPSRTDTFGLVLAEAMACGTPVAAFPVRGPIDVV
                     KDASAGILDDDLGAAAMGALTLDRDKVARYGSRYSWEHSTRQFLSSLVPARSVEAALA
                     EAA"
     gene            1746..2093
                     /locus_tag="VF309_05120"
     CDS             1746..2093
                     /locus_tag="VF309_05120"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX7466004.1"
                     /translation="MVRILHAFFNSIAGLADAWRHESAFRQEILLAIVLIPAACWIPV
                     SAVERALLIGTVLLVMIVELLNTSVEAAIDRISFDQHSLSKRAKDIGSAAVFMALVLL
                     AVVWALVVPPAFL"
     gene            complement(2114..>2741)
                     /locus_tag="VF309_05125"
     CDS             complement(2114..>2741)
                     /locus_tag="VF309_05125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX7466005.1"
                     /translation="LARESGPRPGDERFEEHSDHLVVRDMKSGDVVGAYHILPPRDAR
                     KAGGYFADRDFDLALLIVLRERMVEVDRPCVDPVYPFESVMTHLWSALARYLIENRFD
                     YVFGTADVTLRDGGHCAASIHRMACARFVSPEDYRVFPRHHLPLDSLSNTRPVTLPPL
                     LKGYLDLGAWVCGEPALVTAAARAEFPILLPLARMRGGDARDFLAKAT"
BASE COUNT          439 a          925 c          943 g          434 t
ORIGIN      
        1 tggcagctga agcgccgctg gttctggcag cagagccaca acgacgtgat ccagaaggtg
       61 ctgcgcaagg cgcgcaaggg cacgcgcgtc acctacatcg cgggcaacca cgacgaggcg
      121 atgcgccact tcctcggcct ctcgttcggc ggaatcgaca tccgcgacga agccacgcac
      181 gtgaccgcga gcggcaagcg gctcctcgtg atccacggcg atctattcga cgcagtggtg
      241 cagggcgcga agtggctcgc gcacgccggc gactgggcct acatgatgtc gctcaagctg
      301 aatcgctggt tcaaccatgc gcgcgcgcga gtgggcctgc cctactggtc gctcgcgcag
      361 ttcctcaagc accgcgtcaa gaacgcggtc agctacatcg ggaacttcga ggacgccctc
      421 gcgcacgagg cgcgccggcg cgatttcgac ggcgtggtct gcggccacat ccacaaggcc
      481 gagatccgcg acatcggcgg agtcctctat tgcaacgacg gcgactgggt ggagagcctc
      541 accgcgctgg tggagctcga caacggggag ctgaggatca tcgactggaa ggcgatcgag
      601 gcgttgcgcc gcgatgccgt cgccttcgaa ggagcggaaa ttgcgcattc tcctggtgac
      661 tgacgcctgg gccccgcaga tcaacggcgt ggtggtgacg ctgaggaaca ccgtggcgtg
      721 gctcgagcgg tgggggcacg aagtgcacgt gctctcgccc gagggcttca agacgtttcc
      781 gatgccgacc tatccggaga tcccgctcgc gatctttccc gcgcgcgagg tgaaccggcg
      841 catcgcggcg ctggctcccg acgccatcca catcgcgacc gagggcccgc tgggcatcgc
      901 cgcgcgggcg tactgcgtga agcacgacct ctcgttcacg accgcgtatc acacctgctt
      961 ccccgaatac gtgaagccgc gcttcggcat ccccctcgcg tggacctacg cgtggctgcg
     1021 ccgctttcac ggacgctcgt cggcgctcct ggtcgccacg cccgcgatcc aggcgctgct
     1081 cgaagcgcgc ggcttctcca atatcgccga gtggtcgcgc ggcgtggacc tggagctctt
     1141 caagcccgcg gagcatcgct tctcggacct gcggcgcccg gtattcctgt acgtgggccg
     1201 cgtcgccgtc gagaagaacc ttcccgcgtt cctcgctctc gacctaccgg gcaccaagct
     1261 cgtcgtgggc gacgggccgc aacgcaagga gctcgaggag cgcttccccg atgccgtctt
     1321 cgtgggcgcg aagaccggcc ccgagctcgc gagctactac cagcgcgctg acgtcttcgt
     1381 ctttccgagc cgcaccgata ccttcggcct cgtgctggcc gaagcgatgg cctgcggcac
     1441 gccggtggcg gcatttccgg tgcgcggccc gatcgacgtg gtgaaggacg cgtcggcagg
     1501 cattctcgac gacgatctcg gagcggccgc gatgggggcg ctcacgctgg atcgcgacaa
     1561 ggtggcgcgc tacggcagcc gctactcctg ggaacacagc acgcgccagt tcctgtcgag
     1621 cctcgtcccc gcgcgatccg tggaggccgc ccttgctgaa gccgcctgag gcgagcggtc
     1681 cggcgccgca cgtggcgcgc cccgcagggg agctgaccga gagcccgttc aagggcaaga
     1741 ccggcatggt gaggatcctc catgcgttct tcaattccat cgccgggctg gccgacgcgt
     1801 ggcgccacga gagcgcgttc cgccaggaga ttctcctcgc gatcgtgctg attcccgcgg
     1861 cgtgctggat tcccgtgagc gcggtggagc gcgcgctcct catcggcacg gtgcttctcg
     1921 tgatgatcgt cgaactcctg aacacgagcg tcgaggccgc gatcgaccgc atttcgttcg
     1981 atcagcactc gctctcgaag cgcgccaagg acatcggcag cgccgcggtc ttcatggcgc
     2041 tggtgctgct cgccgtggtg tgggcgctgg tcgtgccgcc cgcgttcctc taggccgcgg
     2101 ccgattcgcg agctcaggtc gccttcgcga gaaaatcgcg cgcgtcgccg ccgcgcatgc
     2161 gcgccagcgg aagcaggatc ggaaactccg cccgggccgc cgcggtgacg agcgcgggct
     2221 cgccgcacac ccacgcacca aggtcgaggt agcccttgag gagaggaggc agggtcaccg
     2281 gccgcgtgtt cgagaggctg tcgaggggaa gatggtggcg cgggaagacg cggtagtcct
     2341 cggggctcac gaagcgcgcg caggccatgc gatggatcga cgcggcgcag tggccgccat
     2401 cgcgaagggt gacgtccgcg gtgccgaaga catagtcgaa gcggttttcg atgaggtagc
     2461 gcgcgagcgc cgaccacagg tgtgtcatca cgctctcgaa cggatagacc gggtcgacgc
     2521 atggacgatc gacctccacc atgcgctcgc gaagcacgat gagcagcgcg aggtcgaaat
     2581 cccggtcggc gaagtagccg cccgccttgc gcgcgtcacg gggcgggaga atgtggtacg
     2641 cgccgacgac atccccggac ttcatgtcgc gcacgacgag gtggtcgcta tgctcctcga
     2701 accgctcgtc gccgggacgc ggcccgcttt cgcgggcgag g
//
LOCUS       DASVII010000392         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_341929,
            whole genome shotgun sequence.
ACCESSION   DASVII010000392 DASVII010000000
VERSION     DASVII010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341929"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF309_05130"
     CDS             <1..196
                     /locus_tag="VF309_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466006.1"
                     /translation="SRQGMGGGAEREAPGPEREGASVREGAAARDGSAGREAASAKGA
                     AKPAKAAGGKFDDFEDDIPF"
     gene            206..1081
                     /gene="htpX"
                     /locus_tag="VF309_05135"
     CDS             206..1081
                     /gene="htpX"
                     /locus_tag="VF309_05135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEX7466007.1"
                     /translation="MKRIVLFLITNIAVMLVLSITLSILGVNRYLTANGLNMGSLLVF
                     SMIVGFTGSIISLLLSKTMAKWSTGARVIDAPATPDEQWLVSAIKHLAEKAGVGMPEV
                     AIYEGGPNAFATGAFRDSSLVAVSTGLLESMTRDEVEAVLGHEIGHVANGDMVTLALI
                     QGVVNTFVIFLARVVGYFVDKTVFRTERGVGMGFYITVFVCEILFGILASLIVAWFSR
                     QREFRADAASAGLLGSPRPMVSALRRLGGLEGGALPQALQAFGIAEGESRFMSLFASH
                     PPIPERIAALENQRP"
     gene            1081..>1584
                     /locus_tag="VF309_05140"
     CDS             1081..>1584
                     /locus_tag="VF309_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7466008.1"
                     /translation="MPLALIVVTVVLAHTAYNGCRLNISLTALASGATPLTVGVLMSL
                     IAALPMLLGVPAGRLVDRIGVRVPIIAATGLLIVAVIVPGVFPGMVALHFAAVGIGSG
                     FALFHICAQHMVGEMSSDERRRDNFGWLALGFATSNFIGPTFSGFSIDTFGNRATFFF
                     LALFSACA"
BASE COUNT          215 a          547 c          534 g          288 t
ORIGIN      
        1 ctcgcgccag ggcatgggcg gcggcgccga acgtgaagcg ccggggcccg agcgcgaagg
       61 cgcgtccgtt cgcgagggcg cggccgctcg tgacggctcg gcgggtcgtg aggccgcttc
      121 cgcgaagggc gcggcgaagc ccgccaaggc cgcgggcgga aagttcgacg acttcgaaga
      181 cgacattccc ttctaggcaa gcgccatgaa aagaatcgtg ctcttcctca tcaccaacat
      241 cgcggtgatg ctggtgctgt ccatcacgct gtcgatcctg ggcgtgaacc gctacctcac
      301 ggcgaacggg ctgaacatgg gctcgctcct cgtgttctcg atgatcgtgg gtttcacggg
      361 ctcgatcatc tcgctgctgc tgtccaagac gatggccaag tggtcgaccg gggcgcgcgt
      421 gatcgatgcc ccggcaacgc ccgacgagca atggctcgtg agcgcgatca agcacctcgc
      481 ggagaaggcg ggcgtcggca tgcccgaggt cgcgatctac gagggcggcc cgaacgcttt
      541 cgccacggga gcgttccgcg attcgtcgct ggtggccgtg tcgacggggc tcctcgagtc
      601 gatgacgcgc gacgaggtcg aggcggtgct cggccacgag atcggccacg tcgccaacgg
      661 cgacatggtg accctggcgc tcatccaggg cgtcgtgaac accttcgtca tcttcctcgc
      721 gcgcgtcgtc ggatacttcg tcgacaagac ggtgttccgc acggagcgcg gcgtgggcat
      781 gggcttctac atcacggtat tcgtttgcga gatcctcttc ggcatcctcg cctcgctcat
      841 cgtcgcgtgg ttctcgcgcc agcgcgagtt ccgcgccgac gccgcgtcgg cggggctcct
      901 cggctcgccg cgcccgatgg tctcggcgct gcgccgcctc ggcggcctcg aggggggagc
      961 ccttccgcag gcgctccagg ccttcggcat cgccgagggc gagagccgct tcatgtcgct
     1021 cttcgcgagc catcccccga tccccgagcg catcgcggcg ctggagaatc agcgaccctg
     1081 atgccgttag cgctcatcgt cgtcacggtc gtactcgccc acaccgcgta caacggctgc
     1141 cgcctcaaca tctcgctcac cgcgctcgcc tcgggcgcga cgcccctcac ggtgggggtg
     1201 ctgatgtcgc tcatcgccgc gctgccgatg ctgctgggcg tgccggcggg ccgtctcgtc
     1261 gatcgcatcg gcgtgcgcgt gccgatcatc gcggcaacgg gcctgctcat cgtggcggtg
     1321 atcgtgccgg gagtctttcc gggaatggtc gcactgcatt tcgccgcggt gggcatcggg
     1381 tccggcttcg cgctcttcca catctgcgcg cagcacatgg tgggagagat gagcagcgac
     1441 gagcgccggc gcgacaactt cgggtggctc gcgctcggct tcgcgacctc caatttcatc
     1501 gggcccacct tctccgggtt ctcgatcgac acgttcggca accgcgccac gttcttcttc
     1561 ctcgcgctct tctcggcgtg cgcg
//
LOCUS       DASVII010000393         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_197492,
            whole genome shotgun sequence.
ACCESSION   DASVII010000393 DASVII010000000
VERSION     DASVII010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197492"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="VF309_05145"
     CDS             <1..1743
                     /locus_tag="VF309_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7466009.1"
                     /translation="TVFQPIFGFREGGVIGYEALVRGPEGSLIQAPAELFAAARADGV
                     ALELNLMCVQEILRAFAERKLPGSVFVNASPQLIVQRGFDQQRAGRFLESLGIAPERV
                     VIELTEDYPTLDFRLVHESLMLYRSMGFRVAIDDLGEGFASLRLWSELKPEYVKADKH
                     FVTGIAEDPVKVQFLRAIQHIAENSASQVIAEGIENAADFKVVKDIGIACGQGWFIGR
                     PSPHPDGKLPREAQLAQADARVPVIPAPRLQAGTEPRAQDFVRTVEVASPQASLASLL
                     DQFAFAPTLASIPVMGGGGIEGVVSRAWLDFVRASPSAQQMRARPCIEFADRAPIKVE
                     AQLDLAALTAILVESDARRMADGFVIVSGGRYLGMGASGDVMRSLQSSRVLAARYTNP
                     LTLLPGQVPINEHLDRLLSGRVPFTAWFIEVDQMRGLNDSEGFATGDALIHATARLVE
                     SACEHGIDFAGHVSGSRFVLLMQSDDWRERAERLLARFPEVIDLHVPAAARERGYFTV
                     KRRDGRDYVRPLPKLVIGVLPVLPGVFESRHEVVAVAKRAAQLALAQPASAMYVDEQH
                     GNAYPQSLLFGNDA"
     gene            complement(1713..>2233)
                     /locus_tag="VF309_05150"
     CDS             complement(1713..>2233)
                     /locus_tag="VF309_05150"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7466010.1"
                     /translation="RDFVARSAAPRRTLVPGCGSAWDVRFLAESGWDVLGIDFSDAAL
                     ATAAPILGPWAARARHADFFAPIAGEPFEVVYERAFLCALPRRMWTAWSKRVADLTEP
                     GGVLAGFFFFGEGERGPPFALSSRQDLSALLEPAFSRIEDSPGTDSIPVFAGKERWQV
                     WRRHASLPKRSD"
BASE COUNT          341 a          785 c          783 g          324 t
ORIGIN      
        1 acggtcttcc agccgatctt cggcttccgc gaaggcggcg tcatcggcta cgaggcgctg
       61 gtgcgaggcc cggaaggctc cctcatacag gcgccggcgg agttgttcgc cgcggcgagg
      121 gcggacggcg tcgccctcga gctgaacctg atgtgcgtcc aggagatcct gcgcgcgttc
      181 gcggaacgca agctcccggg gagcgtcttc gtcaacgcgt cgccgcagct catcgtgcag
      241 cgcggcttcg accagcaacg cgccgggcgt ttcctcgaaa gcctcgggat cgcgccggag
      301 cgcgtggtga tcgagctgac cgaggactat cccacgctcg acttccgcct cgtgcacgag
      361 tcgctgatgc tttaccgctc gatgggcttc cgcgtcgcga tcgacgatct cggcgaaggg
      421 ttcgcgagcc ttcgactgtg gtcggagctc aagcccgagt acgtgaaggc cgacaagcac
      481 ttcgtgacgg gaatcgccga ggacccggtg aaggtgcagt tcctgcgcgc gatccagcac
      541 atcgccgaga acagcgcttc gcaggtcatc gccgagggca tcgagaacgc cgccgacttc
      601 aaggtggtga aggacatcgg catcgcttgc gggcagggct ggttcatcgg gcgtccctcg
      661 ccccatccgg acgggaagct tccgcgcgaa gcgcagctcg cgcaggccga tgcgcgggtg
      721 ccggtgatcc ccgcgccgcg cctgcaggcg ggcaccgagc ctcgcgcgca ggacttcgtg
      781 cgcacggtgg aagtggcctc gccgcaggcg agcctcgcct cgcttctcga ccagttcgcg
      841 ttcgcgccga ccctcgcctc gatccccgtg atgggcggcg ggggaatcga gggcgtcgtc
      901 tcgcgggcgt ggctcgactt cgtgcgcgcg agcccgtcgg cgcagcagat gcgcgcgcgc
      961 ccctgcatcg agttcgcgga ccgcgcgccg atcaaggtgg aggcgcagct cgacctcgcg
     1021 gcgctcaccg cgatcctcgt cgagtccgac gcgcgccgca tggccgacgg gttcgtgatc
     1081 gtctccggcg ggcgctacct cggcatggga gctagcggcg acgtcatgcg ctcgctgcaa
     1141 agctcgcgcg tgctcgccgc gcgctacacc aatccgctca cgctcctgcc ggggcaggtg
     1201 cccatcaacg agcatctgga ccggctgctt tcggggcggg tgccgttcac cgcgtggttc
     1261 atcgaggtcg accagatgcg cggcctgaac gacagcgagg gcttcgccac cggggacgcg
     1321 ctcatacacg ccaccgcgcg actcgtcgaa tcggcgtgcg agcacgggat cgatttcgcg
     1381 ggccatgtct ccggaagccg cttcgtgctg ctcatgcaaa gcgacgactg gcgcgagcgc
     1441 gcggaaaggc tcctcgcccg cttccccgag gtgatcgacc tccacgtgcc cgccgcggcg
     1501 cgcgagcgcg gctacttcac cgtgaagcgt cgcgacggtc gcgactacgt gcgcccgctg
     1561 ccgaagctcg tgatcggcgt gctgccggta ttgcccggcg tattcgaatc gcgccacgag
     1621 gtggtcgcgg tcgcgaagcg cgccgcccaa ctggcgctgg cgcagcccgc gagcgcgatg
     1681 tacgtcgacg agcagcacgg caacgcctat cctcagtcgc tcctcttcgg caacgacgca
     1741 tgacgccgcc acacctgcca gcgctccttg ccggcgaaca ccgggatcga atccgtgccc
     1801 ggcgagtctt cgatgcgcga gaaggcgggc tcgaggagcg ccgagagatc ctgccgggaa
     1861 gatagcgcga agggcggccc gcgctcgccc tcgccgaaga agaagaaacc cgcgaggacg
     1921 ccgccgggct cggtgaggtc ggccacccgc ttcgaccagg cggtccacat gcggcgcggc
     1981 agcgcgcaga ggaacgcccg ctcgtagacc acttcgaaag gctctcccgc gatcggcgcg
     2041 aagaagtcgg catgcctcgc gcgcgcggcc cacggcccga ggatcggcgc ggcggtggcg
     2101 agcgccgcat cgctgaagtc gatgccgaga acgtcccagc cgctctcggc gaggaagcgc
     2161 acgtcccagg cgctgccgca tcccggaacg agcgtgcggc gcggcgccgc ggagcgcgcg
     2221 acgaagtcgc gca
//
LOCUS       DASVII010000394         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_449466,
            whole genome shotgun sequence.
ACCESSION   DASVII010000394 DASVII010000000
VERSION     DASVII010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449466"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="aceE"
                     /locus_tag="VF309_05155"
     CDS             <1..594
                     /gene="aceE"
                     /locus_tag="VF309_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919504.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX7466011.1"
                     /translation="MNENYPHPGLPASAREGILKGMYRISDGGAATGARVQLMGSGTI
                     LREVMFAADLLKKDWGVSADVWSCPSFSELRRDGIAAARWNLLHPTEKPRASYVEQCL
                     AGTKGPVIASTDYVRSFADQIREFVPRRYKVLGTDGFGRSDSRDNLRRFFEVNRHYVA
                     VAALKALAEDGEIAPAKVAEAIRKYGIDTEKPAPWTV"
     gene            625..1956
                     /gene="aceF"
                     /locus_tag="VF309_05160"
     CDS             625..1956
                     /gene="aceF"
                     /locus_tag="VF309_05160"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEX7466012.1"
                     /translation="MAAFEVRVPDIGDFKDVPVIEVLVKPGDSVKKDDSLLTLESDKA
                     TMEVPAPSSGTVKTILVKVGDKVSEGVAILTFEGDAAAKAAPAKAEAPATKAAPPQPK
                     APPAPRAAAPAAAAAQPAASAAPIAPAPALAAPDSGTPPHASPGVRRFARELGVEVAK
                     VSGSGPKGRILKDDIQNFVKQALSGGAAPSAAARAGGMSDLGLPAWPKVDFPKFGPIE
                     TKPLTRIQKISGPALTRNWVMIPHVTQFDEADITELEAFRAKVNEENAKSGVKVTPLA
                     FLIKAVVAALKKYPTLNSSLDGDNLVVKQYWNIGFAADTPNGLVVPVVKSADQKGVIE
                     IAKETSDLAAKAREGKLGPAEMQGGTFSISSLGGIGGTSFTPIVNAPEVAILGVSKAA
                     MKPVWNGKEFAPRLMMPLSLSYDHRVVDGALGARFTAFLAQVIADLRRGML"
     gene            1966..>2267
                     /locus_tag="VF309_05165"
     CDS             1966..>2267
                     /locus_tag="VF309_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7466013.1"
                     /translation="MATIEVKVPDIGDFKDVPVIEILVQPGDAVRKEDSLVTLESDKA
                     TMEVPSTHSGTVKEVKVKVGDRVAQGTLIAILDTAASADAPAPPASAAAPAQAPVQP"
BASE COUNT          411 a          771 c          775 g          310 t
ORIGIN      
        1 atgaacgaga actacccgca ccccggcctt ccggcaagcg cgcgcgaggg aatcctgaag
       61 ggcatgtacc ggatctccga tgggggcgcc gccaccgggg cacgcgtgca gttgatggga
      121 agcggcacga tccttcgcga ggtgatgttc gccgccgacc tgctgaagaa ggactggggc
      181 gtgtcggccg acgtgtggag ctgcccgagc ttcagcgagc ttcgccgcga cggcatcgcc
      241 gccgcgcgat ggaatctcct gcatccgacc gagaagccgc gcgcctcgta tgtcgagcag
      301 tgcctcgccg gcacgaaggg cccggtgatc gcctcgaccg actacgtgcg cagcttcgcc
      361 gaccagatcc gcgagttcgt gccgcgccgc tacaaggtgc tcggcaccga cggcttcggg
      421 cgcagcgact cgcgcgacaa cctgcgccgc ttcttcgagg tgaatcgcca ctacgtcgcc
      481 gtcgccgcgc tgaaggcgct cgccgaggac ggcgagatcg cgcccgcgaa ggtcgcggag
      541 gcgatcagga aatacggcat cgacacggag aagcccgcgc cctggacggt gtgattggcc
      601 acaacgaaat taaggagaag caagttggcc gcattcgaag tcagggttcc cgacatcggg
      661 gacttcaagg acgtccccgt gatcgaggtg ctggtgaagc cgggcgattc cgtcaagaag
      721 gacgactcgc tcctcacgct cgaaagcgac aaggcgacga tggaggtgcc ggcgccctct
      781 tccggcaccg tgaagacgat cctcgtgaag gtgggcgaca aggtgagcga gggcgtcgcg
      841 atcctgactt tcgaaggcga cgcggcggcg aaggctgcgc ccgcgaaggc tgaagcgccc
      901 gcgacgaagg ccgcgccccc acagccgaag gcgcccccgg ccccgcgagc cgctgcgccc
      961 gccgccgcgg ccgcgcaacc ggcggcaagc gcagcgccca ttgcgccagc acccgcattg
     1021 gcggcaccgg attccggcac tccgccgcac gcgagccccg gcgtgcgccg cttcgcacgc
     1081 gagctcggcg tcgaggtcgc gaaggtttcg ggatccggcc cgaaaggacg catcctcaag
     1141 gacgacatcc agaatttcgt gaagcaggcg ctatcggggg gcgcggcgcc gtccgcggca
     1201 gcccgcgccg gcggcatgag cgatctcggg cttcccgcat ggcccaaggt cgacttcccc
     1261 aagttcggtc cgatcgagac gaagccgctc acgcgcatcc agaagatctc gggcccggcg
     1321 cttacgcgca actgggtgat gatcccgcac gtgacgcagt tcgacgaggc ggacatcacc
     1381 gagctcgagg cctttcgcgc gaaggtgaac gaggagaacg cgaagtccgg agtgaaggtg
     1441 acgcccctcg cgttcctcat caaggcggtg gtcgcggcgc tgaagaagta tccgacgctc
     1501 aacagctcgc tcgacggcga caacctcgtc gtcaagcaat actggaacat cggcttcgcc
     1561 gccgacacgc ccaacggcct cgtcgtgccc gtcgtgaaat cggcggacca gaagggcgtg
     1621 atcgagatcg cgaaggaaac ctcggacctc gccgcgaaag cgcgcgaggg caagctgggc
     1681 cccgccgaga tgcagggcgg aacgttctcg atctcgtcgc tcggcggcat cggcggcacg
     1741 tcgttcacgc cgatcgtgaa cgcgcccgag gtcgcgatcc tcggcgtctc gaaggccgcg
     1801 atgaagcccg tgtggaacgg caaggagttc gcgccccgcc tcatgatgcc gctctcgctt
     1861 tcgtacgacc atcgcgtcgt cgacggcgcg ctcggcgccc gattcaccgc cttcctcgcc
     1921 caggtcatcg ccgacctgcg gcgcggcatg ctgtaaggga cggccgtggc gacgatcgag
     1981 gtcaaggtcc ccgacatcgg cgacttcaag gacgtgccgg tcatcgagat cctggtgcag
     2041 ccgggcgacg cggtgcgcaa ggaagattcc ctcgtcacgc tcgaaagcga caaggcgacg
     2101 atggaagtgc cctctacgca ctcgggcacg gtgaaggaag tgaaggtcaa ggtcggcgat
     2161 agggtcgcgc agggaacgct gatcgcgatc ctcgatacgg ccgcatccgc cgacgcgccc
     2221 gcgccgccgg cgagcgccgc cgcccccgcg caggcgccgg tccagcc
//
LOCUS       DASVII010000395         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_485530,
            whole genome shotgun sequence.
ACCESSION   DASVII010000395 DASVII010000000
VERSION     DASVII010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485530"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VF309_05170"
     CDS             <1..120
                     /locus_tag="VF309_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEX7466014.1"
                     /translation="GRVKISIGLAKGKKQHDKREVEKERDWQREQQQLLRRKA"
     gene            complement(184..258)
                     /locus_tag="VF309_05175"
     tRNA            complement(184..258)
                     /locus_tag="VF309_05175"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:His,seq:gtg)
     gene            complement(266..342)
                     /locus_tag="VF309_05180"
     tRNA            complement(266..342)
                     /locus_tag="VF309_05180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Arg,seq:tct)
     gene            complement(372..445)
                     /locus_tag="VF309_05185"
     tRNA            complement(372..445)
                     /locus_tag="VF309_05185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Pro,seq:tgg)
     gene            complement(472..>1867)
                     /gene="pnp"
                     /locus_tag="VF309_05190"
     CDS             complement(472..>1867)
                     /gene="pnp"
                     /locus_tag="VF309_05190"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7466015.1"
                     /translation="SDLADAYRLKSKSLRQEKLEDIKKRVLDECVVNAEVPADSNVAR
                     GILKDMEAKIVRGQILGGEPRIDGRNTRTVRPISIRPTILPRVHGSVLFTRGETQAIV
                     TTTLGTGQDEQRIDALQGEYTERVMMHYNFPPFSTGETGRVGSPKRREIGHGRLAKRA
                     LLAVLPSKEEFAYTLRVVSEITESNGSSSMASVCGGCLSLMDAGVPLKAHVAGIAMGL
                     IKDGGRFAVLTDILGDEDHLGDMDFKVAGTEQGITALQMDIKITGITREIMKVALDQA
                     KEGRMHILAIMKQAVPGPRVEMSNYAPRIIKMKINPDKIREVIGKGGSVIQALTRETG
                     TTIDIEDDGTISIACLSAEAGEAAQERIRAITAEVEVGKVYEGPVLRLLDFGAIVQLL
                     PGKDGLLHISQIAHERVNAVSDYLKEGQIVKVKVLEADDKGRVRLSRKALLDPPAPKR
                     EESPPPTPAESTSQ"
BASE COUNT          294 a          616 c          606 g          351 t
ORIGIN      
        1 ggacgcgtga agatctccat cgggctcgcc aaggggaaga agcagcacga caagcgcgag
       61 gtcgagaagg agcgcgactg gcagcgcgag cagcagcagc tcctgcgccg caaggcctag
      121 aagcctctcg cgcctcgcgc cgggactctc ggagaaaagc ccgctttcgc gggctttttg
      181 atttggggtg gctgatggga ttcgaaccca cgacgactgg aatcacaatc caggactcta
      241 ccgctgagct acagccaccg ttaactggca cgcccgacag gattcgaacc tgttaccccc
      301 ggcttagaag gccggtgctc tatccggatg agctacgggc gcattccgaa tcgaatcgtc
      361 gcacgcgagg gtcggggtgg agggatttga acccccgaca tcttgctccc aaagcaagcg
      421 cgctaccagg ctgcgctaca ccccgaattc accgaaattg tacgcggccc cttactgcga
      481 tgtcgattcg gccggtgtcg gaggcggaga ctcctcgcgc ttcggcgccg gcggatcgag
      541 gagggccttt ctggagaggc gcacgcggcc cttgtcgtcg gcctcgagca ctttcacctt
      601 cacgatctgc ccttccttca ggtagtccga gaccgcgttg acgcgctcgt gggcgatctg
      661 cgagatgtgc agcaggccgt ccttgccggg cagcaactgc acgatcgcgc cgaagtcgag
      721 gaggcgcagc accgggcctt cgtagacctt gccgacctcg acttccgccg tgatcgcgcg
      781 gatgcgctcc tgcgcggcct ctcccgcttc cgcggaaagg catgcgatgg agatcgtgcc
      841 gtcgtcctcg atgtcgatcg tcgtgcccgt ctcgcgggtc agggcctgga tcacgctgcc
      901 gcccttgccg atcacctcgc ggatcttgtc ggggttgatc ttcatcttga tgatgcgcgg
      961 cgcgtagttc gacatctcga cgcgcggtcc tggcaccgcc tgcttcatga tggcgaggat
     1021 gtgcatgcgc ccttccttgg cctggtcgag cgcgaccttc atgatctcgc gcgtgatgcc
     1081 tgtgatcttg atgtccatct gcagcgccgt gatgccttgc tcggtcccgg ccaccttgaa
     1141 gtccatgtcg cccaggtgat cctcgtcgcc gaggatgtcg gtgagcaccg cgaaacgccc
     1201 gccgtccttg atgaggccca tcgcgatgcc ggcgacgtgg gccttcagcg gcactcccgc
     1261 gtccatcagc gacaggcacc ctccgcacac cgatgccatc gacgaggagc cgttggactc
     1321 ggtgatctcg gagaccacgc gcagcgtata ggcgaactct tccttcgacg gcagcaccgc
     1381 caggagcgcg cgcttggcga gccgtccgtg gccgatctcg cggcgcttgg gcgagcccac
     1441 gcggcccgtc tcgccggtcg agaacggcgg gaagttgtaa tgcatcatga cgcgctcggt
     1501 gtactcgccc tgcagcgcgt cgatgcgctg ctcgtcctgc cccgtgccga gcgtcgtcgt
     1561 gacgatcgcc tgggtctcgc cgcgcgtgaa gagcacgctg ccgtggacgc gcggaagaat
     1621 cgtggggcgg atcgagatgg ggcgcaccgt gcgcgtgttg cgcccatcga tgcgcggctc
     1681 gccgccgagg atctgcccgc gcacgatctt ggcttccatg tccttgagga tgccgcgcgc
     1741 gacgttggag tcggccggga cctcggcatt gaccacgcac tcgtcgagca cgcgcttctt
     1801 gatgtcctcg agcttctcct ggcgcagcga cttcgacttg aggcgatagg cgtcggcgag
     1861 atcgctc
//
LOCUS       DASVII010000396         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_234005,
            whole genome shotgun sequence.
ACCESSION   DASVII010000396 DASVII010000000
VERSION     DASVII010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234005"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..870)
                     /locus_tag="VF309_05195"
     CDS             complement(115..870)
                     /locus_tag="VF309_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7466016.1"
                     /translation="MKARALIADDEAHLAEHLRARLALAWPDLEILPLAANGVEALRA
                     IGEEEPEIAFLDIRMPGLTGIELAKRIDTRTHVVFVTAFDQYAVEAFDREVVDYILKP
                     VTDERLARTVERLQRKLAAAERPPALDDVLARLARAIPGAGARLRWIRALKGELVQQI
                     AVDDVLYFQASDKYTCVVTRDGESLIRLALAELSGQLDSDVFWQIHRGTVVNMNEVAS
                     TRRDLGGRVFVKLKDGKTELAVSRAYAHLFKQM"
     gene            complement(867..>1558)
                     /locus_tag="VF309_05200"
     CDS             complement(867..>1558)
                     /locus_tag="VF309_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7466017.1"
                     /translation="SLVISTIVSLIFFGREREAVAAASLALERERTQRIEREATLANL
                     RALQAQIEPHFLFNTLANVTSLIDPDPAKARRMLESFIRFLRSSLGATRAESTTLADE
                     GELMAAYLDVLQIRMGQRLRYEIDIPPELHAFQLPPMLLQPVVENSIRHGLEPKMDGG
                     VVKVRARREDGAVVIEISDSGAGFAATTRGGVGLTNLRDRLRLLYGERASLAIGESDG
                     GGARVTMSLPA"
BASE COUNT          247 a          515 c          547 g          249 t
ORIGIN      
        1 gccagctcca tctcatggag aagctgggcg aaggtgagcg tggcggggaa ttcgcggggc
       61 accccgttca gcgtgacttg aatgcgcatg agggaattat aagcctcatg cccgctacat
      121 ctgcttgaac aggtgggcgt acgcgcggct cacggcgagc tcggtcttgc cgtccttcag
      181 cttcacgaac acgcgccctc cgagatcgcg gcgcgtcgag gcgacctcgt tcatgttgac
      241 gacggtgccg cgatggatct gccagaacac gtcggagtcg agctgtccgg agagctcggc
      301 gagcgcaagg cggatgagcg actcgccgtc gcgcgtgacg acgcacgtgt acttgtcgga
      361 ggcctggaaa taaagcacgt cgtcgaccgc gatctgctgc accagctcgc ctttcagcgc
      421 gcggatccag cgcaatcgcg ccccagcgcc ggggatggcg cgggcgagcc gggcgagcac
      481 gtcgtccagc gcgggcgggc gctccgccgc ggcgagcttt ctctgcagcc gctcgacggt
      541 cctggcgagg cgctcgtcgg tgacgggctt caggatgtag tcgacgactt cacggtcgaa
      601 agcctcgact gcatattggt cgaaggcggt cacgaagacc acgtgcgtgc gcgtgtcgat
      661 gcgtttcgca agctcgatgc ccgtgagccc cggcatcctg atgtctagga acgcgatctc
      721 gggctcctcc tcgccgatcg cgcgcagggc ttccacgccg ttcgcggcga gcggcaggat
      781 ctcgagatcg ggccaggcga gcgccaggcg ggcgcgcagg tgctcagcca ggtgcgcctc
      841 gtcgtcggcg atgagcgccc gcgccttcat gcgggaaggc tcatcgtcac ccgcgctccg
      901 ccgccatcgc tctcgccgat cgcgagcgag gcgcgctcgc catagaggag ccgcaggcga
      961 tcgcgcaggt tggtgaggcc cacgccgccc cgagtcgtcg cggcgaagcc cgcgccgctg
     1021 tcggagattt cgatgaccac cgcgccgtcc tcgcgccgcg cgcgcacctt caccacgccg
     1081 ccatccatct tgggctcgag gccgtgccgg atagaattct cgacgaccgg ctgcagcagc
     1141 atgggcggca gctggaacgc gtgcagctcg ggaggaatgt cgatctcgta gcgaaggcgc
     1201 tgacccatgc gtatctgcag cacgtcgagg taggccgcca tgagctcgcc ctcgtccgcg
     1261 agcgtggtcg attcggcccg cgtcgcgccg agggaggagc gcaggaagcg gatgaagctc
     1321 tccagcatgc gccgcgcctt ggccggatcg ggatcgatga ggctcgtcac gttggcgagc
     1381 gtattgaaca ggaaatgcgg ctcgatctgc gcctggagcg cgcgcaggtt ggcgagcgtc
     1441 gcctcgcgct cgatcctctg cgtgcgctcg cgctccaggg cgagcgaggc ggccgccaca
     1501 gcctcgcgct cgcgcccgaa gaagatgagg ctcacgatcg tcgagatgac caggctcg
//
LOCUS       DASVII010000397         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_593576,
            whole genome shotgun sequence.
ACCESSION   DASVII010000397 DASVII010000000
VERSION     DASVII010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593576"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..396)
                     /locus_tag="VF309_05205"
     CDS             complement(31..396)
                     /locus_tag="VF309_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466018.1"
                     /translation="MTCLLQLRLAGGLIDIARAMRVLKRYSISQSRVSMAREGSREVA
                     TVMGTLADPRRSVGLAAALSRIPGVLEAVVSREDESVAAFYCAPQRGSRPAQGQYKKS
                     NDRQLLLTGAAVDVEVPCT"
     gene            complement(393..1493)
                     /locus_tag="VF309_05210"
     CDS             complement(393..1493)
                     /locus_tag="VF309_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466019.1"
                     /translation="MKVALKTLAIALAAVSANAFAQVPAGYPAEYAKIVAAAKAEGKV
                     VIYSALDTKAASPLVKDFNALYPDVKVEYNDMNSTELYNRFIAEVASGQGGADVMWSS
                     AMDLQVKLVDDGQAMTYASPEVAKLPKWAVYKNQAYGTTYEPAVFIYNKRLVTGDEIP
                     QDHAAFAKLIASKVDKFKGKVTTYDIEKSGVGFMFVVQDTKYFPGMKDLERGFGATSY
                     KVYSSTGNMLEKVSSGEHLLGYNVLGSYALVRAKKDPNLGVVLPKDFTLVLSRVMFIG
                     KKSQHPNAAKLWVDYVLSQRGQKMIGGDVELFAMRDDVEAEYTAAKLNKQLGGVVKPI
                     PVGAEITEYLDSKKRLDFLAQWKATLATGGGK"
BASE COUNT          273 a          550 c          475 g          325 t
ORIGIN      
        1 cgttcttctt catgtgtagt gcctccactc ttatgtacat ggaacttcaa catcgaccgc
       61 tgccccggta aggagaagct ggcgatcatt cgacttcttg tactgccctt gtgccggtct
      121 ggagcctcgc tgcggagcgc aatagaaggc ggcaacggat tcgtcttcgc gggacacgac
      181 cgcctcgagc actcccggaa tgcgcgaaag agccgcggca aggcccacgg agcggcgcgg
      241 atccgcgagc gtccccatca ccgtcgcgac ctcccgcgag ccctcgcgcg ccatcgagac
      301 gcgcgactgc gagatgctgt aacgcttgag cacgcgcatg gctcgcgcga tgtcgatcag
      361 gccgccggcg agccgcagct gaagcaggca cgtcatttcc cgccgccggt tgcgagagtc
      421 gccttccact gggccaggaa gtcgagccgc ttcttcgaat ccaggtactc ggtgatctcg
      481 gcgccgaccg ggatgggctt caccacgccc ccgagctgct tgttcaactt cgcggcggtg
      541 tactccgcct ccacgtcgtc cctcatcgcg aagagctcca catcgccccc gatcatcttc
      601 tgcccgcgct gcgagagcac gtagtccacc caaagcttcg ccgcgttcgg gtgctggctc
      661 ttcttgccga tgaacatcac ccgcgacagg accagcgtga agtccttggg cagcaccacc
      721 cccaggttcg gatccttctt cgcgcgcacc agcgcatagg agcccagcac gttgtagccc
      781 agcaggtgct cgcccgagga gaccttctcc agcatgttgc cggtgctcga atagaccttg
      841 tacgaggtcg cgccgaagcc gcgctcgaga tccttcatgc cggggaagta cttcgtgtcc
      901 tgcaccacga acatgaaccc cacgccgctc ttctcgatgt cgtaggtggt caccttcccc
      961 ttgaacttgt ccaccttgga ggcgatcagc ttcgcgaacg ccgcgtgatc ctgcggaatc
     1021 tcgtcgcccg tcaccaggcg cttgttgtag atgaaaaccg ccggctcgta ggtggtcccg
     1081 tacgcctggt tcttgtacac cgcccacttc ggcagcttcg caacctcggg cgaggcatag
     1141 gtcatcgcct gcccgtcgtc gaccagcttc acctgcaggt ccatcgccga gctccacatc
     1201 acgtcggcgc cgccctgccc cgaggccacc tccgcgatga agcggttgta gagctcggtc
     1261 gaattcatgt cgttgtactc gaccttcacg tcgggataga gcgcgttgaa gtccttcacc
     1321 agcggtgaag ccgccttggt gtccagcgcg ctgtagatca ccaccttgcc ttcggccttc
     1381 gccgccgcca cgatcttcgc gtattcggcc ggataacccg cgggaacttg cgcgaatgcg
     1441 tttgcggaga ctgcggcgag cgcgatcgcc agggtcttga gtgcgacttt catggaattc
     1501 tcctgggatc gcacgctact tgggagcgta ggtgaggacc gtgaaggtgt ccgggaaata
     1561 gcggttcgga tagaacggcg ccacgccttt gccgatcttc ttcgcgggat cggcggtgcc
     1621 ctc
//
LOCUS       DASVII010000398         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_378260,
            whole genome shotgun sequence.
ACCESSION   DASVII010000398 DASVII010000000
VERSION     DASVII010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378260"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VF309_05215"
     CDS             complement(<1..1048)
                     /locus_tag="VF309_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX7466020.1"
                     /translation="MDTIHALAGVAALVILSWAVSENRRGVHWRLVASGIAMQVVLAA
                     LLLLVPWLRDGVFSLNTALEALERATQAGTTFVFGYLAGGPTPFAQVDPAASFVLAFR
                     ALPLVLVVSALSALLFPWRILPFVVRGFGWLLEKSMGVGGAVGLSAAANVFVGMVEAP
                     LVIKPYLLRLTRSELFIVMVCGMATIAGTVMALYGAILAPVVPEAIGHILIASIVATP
                     AAITVAALMVPGEMTRGAAVSIERQDSSAMDAVTRGTIEGVALLIQIVAMLIVLVALV
                     YLVNSALAILPAWDGEPITLQRGLGWIFAPLAWSAGVPWSEAPAAGALLGTKTALNEF
                     IAYLELAKLPPEVLS"
     gene            1189..2328
                     /gene="carA"
                     /locus_tag="VF309_05220"
     CDS             1189..2328
                     /gene="carA"
                     /locus_tag="VF309_05220"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7466021.1"
                     /translation="MPQKTPAVLVLADGSVFRGVGIGGAGQTVGEVVFNTAMTGYQEI
                     LTDPSYAGQIVTLTYPHIGNTGTNAEDVESRAVYAAGLVIRDLPLAASSWRRSEDLST
                     YLARNSIVAIGDIDTRRLTRILRSKGAQNGCIQAGAVDEKVALAAAKAAPSMAGQDLA
                     KTVSVDKPYDWNGSLWALGTGYKPDGEAKYHVVAYDYGVKHNILRNLAERGCKVTVLP
                     AMASAGDSLRLKPDGVFLSYGPGDPEPCDYAIKAIREIVGTGTPVFGICLGHQLLGLA
                     AGAKTMKMKFGHHGANHPVQDLASGRVFITSQNHGFAVDESTLPSNVRPTHRSLFDGS
                     LQGIEYTDKPAYSFQGHPEASPGPREMAYLFDKFVSMMAAKRKAG"
BASE COUNT          464 a          877 c          820 g          335 t
ORIGIN      
        1 gcgagaggac ctcgggcgga agcttggcga gctcgaggta cgcgatgaac tcgttcagcg
       61 ccgtcttggt gccgagaagc gcgcccgcgg ccggcgcctc gctccagggc actccggcgg
      121 accaggcaag cggtgcgaag atccagccga ggccgcgctg cagcgtgatg ggctcgccgt
      181 cccacgccgg caggatcgcg agcgcggagt tcaccaggta cacgagcgcc acgagcacga
      241 tcagcatcgc cacgatctgg atgaggagcg ctaccccttc gatggtgccg cgcgtgacgg
      301 cgtccatcgc gctcgaatcc tggcgctcga tcgacacggc cgcgccgcgc gtcatctcgc
      361 cgggcaccat cagcgcggcg accgtgatcg ccgcgggtgt ggcgacgatc gaggcgatga
      421 gaatgtggcc gatggcctcg ggcaccacgg gagcgaggat cgcgccgtag agcgccatca
      481 ccgttccggc gatcgtcgcc atgccgcaca ccatcacgat gaagagctcg ctgcgcgtca
      541 ggcgaaggag atagggcttg atgacgaggg gcgcctcgac catgccgacg aagacgttgg
      601 cggccgccga gagtcccacc gcgccgccca cgcccatcga cttctccagg agccagccga
      661 agccgcggac gacgaacggc aggatgcgcc aagggaagag cagcgccgag agcgcgctca
      721 ccaccagcac cagcgggagc gcgcggaagg cgagcacgaa ggaggcggcg ggatcgacct
      781 gcgcgaatgg cgtcggcccg cccgcgagat agccgaacac gaaggtcgtg ccggcctgcg
      841 tcgcgcgctc gagcgcctcg agcgcggtgt tcagcgagaa cacgccgtcg cgcagccatg
      901 gaacgaggag cagcagcgcc gcgaggacca cctgcatcgc gatgccggag gcgaccaggc
      961 gccagtggac gccgcgccgg ttctccgaga ccgcccacga gaggatgacg agggccgcga
     1021 ccccggccag tgcgtgaatg gtgtccattg gcctcgaatt atgtccgctt ttctgctaca
     1081 atcttcgacc aatgaaagcg ggacgagcca ctagagattt gcgctctccc gcattttttt
     1141 gcccgctgta ttcgccgccg atttgtaaat agacccacgg agccgttagt gccgcagaag
     1201 acccccgccg tcctggtgct cgccgacggg agtgtttttc gcggcgtcgg catcggcggg
     1261 gcgggccaga ccgtcggcga ggtggtcttc aacaccgcga tgacgggcta ccaggagatc
     1321 ctcaccgatc cctcgtacgc cggccagatc gtcaccctca cctatccgca catcggcaac
     1381 accggcacca acgccgagga tgtcgaatcg cgcgcggtct acgccgcggg cctcgtcatc
     1441 cgcgatcttc cgctcgccgc ctccagctgg cgccggagcg aggacctctc gacctacctc
     1501 gcgcgcaact cgatcgtcgc catcggcgac atcgacacgc gcaggttgac gcgcatcctg
     1561 cgcagcaagg gcgcgcagaa cggctgcatc caggccggcg ccgtcgacga gaaggtggcg
     1621 cttgccgccg cgaaggccgc gcccagcatg gcgggccagg acctcgcgaa gaccgtgagc
     1681 gtcgacaaac cctacgactg gaacggcagc ctctgggcgc tgggcaccgg ctacaagccc
     1741 gacggcgagg cgaagtacca cgtggtcgcg tacgactatg gcgtcaagca caacatcctc
     1801 aggaacctcg ccgagcgcgg ctgcaaggtg acggttctgc ccgccatggc cagcgcgggc
     1861 gactcgctga gactcaagcc cgacggcgtc ttcctctcct acggccccgg agacccggag
     1921 ccgtgcgact acgcgatcaa ggcgatccgc gagatcgtcg gcaccggcac gccggtgttc
     1981 ggcatctgcc tcggccacca gctcctcggc ctcgccgcgg gcgcgaagac catgaagatg
     2041 aaattcggcc accacggcgc gaaccacccg gtgcaggacc tcgccagcgg ccgcgtcttc
     2101 atcaccagcc agaaccacgg cttcgcggtc gacgaatcca cgctgccgtc caacgtgcgg
     2161 cccacgcacc gctcgctctt cgacggctcg ctgcagggga tcgaatacac cgacaagccc
     2221 gcgtacagct tccagggcca ccccgaggcg agccccggcc cgcgcgagat ggcctacctc
     2281 ttcgacaagt tcgtctcgat gatggccgcc aagagaaaag ccggatagcc cctcatgccc
     2341 aagcgcagcg acatcaagag catcctcatc atcggcgcgg gcccgatcgt gatcggccag
     2401 gcgtgcgagt tcgactactc gggcgcgcag gcgtgcaagg cgctgaggga cgagggctac
     2461 aaggtgatcc tggtcaactc caacccggcc acgatc
//
LOCUS       DASVII010000399         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_378278,
            whole genome shotgun sequence.
ACCESSION   DASVII010000399 DASVII010000000
VERSION     DASVII010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378278"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..968
                     /locus_tag="VF309_05225"
     CDS             84..968
                     /locus_tag="VF309_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7466022.1"
                     /translation="MAGQVILGKERQIRLAMACILARGHLLIEDVPGVGKTTLSHALA
                     RLLGLDYQRIQFTSDLLPADIIGVSIFDRDSSTFRFHAGPIFAQIILADEINRASPKA
                     QSALLEAMEEHQVTAEGETRKLPEPFFVIATQNPMHQVGTFPLPESQLDRFLMRINLG
                     YPDAKAERELLKGAERRDMIQHLDRAMSTAQLVELQKAVPQMQVSDALLDYVQQIINH
                     TRISPRFTQGLSPRAGVAILRAAQAWAMMEGRRQVLPEDVQAILPAVVGHRLHGSSDA
                     AKGTFDPARELIAGVPIP"
     gene            1175..>1627
                     /locus_tag="VF309_05230"
     CDS             1175..>1627
                     /locus_tag="VF309_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7466023.1"
                     /translation="MFADLRQSISDWIFRASAPESSPVTLVQRRIFILPTRQGYTFAL
                     TMLVLLLASINYALSLGFVLTFTLVSMAGVAMLHTWRNLAHLKLRPGRCDPVFSGEVA
                     HFGVTVETPAQTRFSIAIRRKGEEPVYADVAPAEIAAIMLPFATPRRGI"
BASE COUNT          258 a          589 c          497 g          283 t
ORIGIN      
        1 accgctgaca gggttccatt ttttccatgc ttctggatgc acgcagccgg ctctccgccg
       61 gccccaccga ggcggtcatc gcgatggccg gccaggtcat cctcggcaag gaacgccaga
      121 tccggctcgc catggcgtgc atcctggcgc gcggccacct gctgatcgag gacgtgccgg
      181 gcgtcggcaa gacgacgctt tcgcacgcgc tggcgcgcct cctggggctc gactaccagc
      241 gcatccagtt cacctccgac ctcctgcccg ccgacatcat cggcgtgtcg atcttcgacc
      301 gcgacagctc gactttccgc ttccacgcgg gcccgatctt cgcgcagatc atcctggctg
      361 acgagatcaa ccgcgcgagt ccgaaggcgc agagcgcgct tctggaagcc atggaggagc
      421 accaggtgac cgcggagggc gagacgcgca agcttcccga gcccttcttc gtgatcgcga
      481 cgcagaatcc gatgcaccag gtcgggacct ttccccttcc ggaatcgcag ctcgaccgct
      541 ttctcatgcg catcaacctg ggctatcccg acgcgaaggc cgagcgcgag ctgctgaagg
      601 gcgccgagcg cagggacatg atccagcacc tcgaccgcgc gatgagcaca gcgcagctcg
      661 tcgagctgca gaaggcggtg ccgcaaatgc aggtctccga cgcgctcctc gactacgtcc
      721 agcagatcat caaccacacg cgcatctcgc cgcgcttcac gcagggcctg tcgccgcgcg
      781 ccggcgtcgc gatcctgcgc gccgcccagg cgtgggcgat gatggagggg cgccggcagg
      841 tgcttcccga ggacgtgcag gcgatcctgc ctgcggtcgt cggccatcgc ctgcacggca
      901 gctccgacgc cgcgaagggc actttcgatc ccgccaggga gttgatcgcc ggcgttccga
      961 ttccctgatc ctgcgcgtgc cgcattcaag cgtgatccgc agatgaacgc agatgaacgc
     1021 agtcaacccc acacaggtcc ccgagagttt cgtgcctgcg cttggcgatg gcgccctgtc
     1081 attcgaagat ccactctcca aggtctcatc tgcgttcatc tgcgtttatc tgcggatcca
     1141 aagccttctg ctcgcatgac tctccggtga aaccgtgttc gccgacctga ggcagtcgat
     1201 ctcggactgg atctttcgcg cgagcgcgcc cgaaagctcg ccggtcacgc tcgtccagcg
     1261 ccgcatcttc atcctgccga cgcgccaggg atacaccttc gcgctgacga tgctggtgct
     1321 cctcctcgcc tcgatcaact acgcgctctc cctcggcttc gtgctcacct tcaccctcgt
     1381 gagcatggcg ggcgtggcga tgctgcacac gtggcgcaac ctcgcgcacc tcaagctgcg
     1441 cccggggcgc tgcgacccgg tcttctcagg cgaggtcgcg cacttcgggg tgacggtcga
     1501 gacgcccgcg cagacgcgct tttcgatcgc gatccgccgc aagggcgagg agccggtgta
     1561 cgccgacgtc gcgcccgccg agatcgccgc gatcatgctg ccgttcgcca cgccgcggcg
     1621 cggcatc
//
LOCUS       DASVII010000400         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_701605,
            whole genome shotgun sequence.
ACCESSION   DASVII010000400 DASVII010000000
VERSION     DASVII010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701605"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VF309_05235"
     CDS             <1..747
                     /locus_tag="VF309_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7466024.1"
                     /translation="PPVRVAERDAPEAAPHFRMPILFEDESILAIDKPAGLAVHGGSG
                     VAHGVIESLRSIRPEAKFLELVHRLDRDTSGVLLLAKKRSALVVLHEMMRGHDMEKRY
                     LAGVAGRFRNEKQRVRLSLSKHVNAAGEKRVSVSDAGQEAETIFHLVERGTEMSLLEA
                     ELLTGRTHQIRVHLAHLGHPVLGDDKYGDFELNHRLRKEGLKRMFLHAAKLSFAHPVT
                     GQRVDLVSPLPADLEKFRSRIKAVDADERR"
     gene            872..>1508
                     /locus_tag="VF309_05240"
     CDS             872..>1508
                     /locus_tag="VF309_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7466025.1"
                     /translation="MNYELVIFDWDGTLMDSTRVIASSLQSACSDVGVPVPSERDALY
                     VIGLNLADTFNHVAPGLDEEGRRRLSERYRHPFLAREHEMPLYDGVVEMLSDLHGKGA
                     RLAVATGKARRGLDRVLDATGLGRWFEATRCADEGFAKPHPGMLLMLMDLTGVEPRRA
                     LMVGDTTHDLELAANAGVDAVAVTYGAHDEKLLGTRPAKARCASVPELHRWL"
BASE COUNT          265 a          505 c          497 g          241 t
ORIGIN      
        1 cctcccgtgc gcgtggccga gcgcgacgcg ccggaagccg cgccgcattt ccgcatgccg
       61 atcctcttcg aggacgagtc catcctcgcg atcgacaagc cggccggact cgcggtgcac
      121 ggcggcagcg gagtcgccca cggcgtgatc gaatcgctgc gctcgatccg ccccgaggcg
      181 aagttcctcg agctggtgca ccggctcgac cgcgacacat ccggcgtgct gctgctcgcc
      241 aagaaacgca gcgccctcgt cgtcctgcac gagatgatgc gcgggcacga catggagaag
      301 cgttatctcg cgggagtcgc cgggcgcttt cgcaacgaga agcagcgcgt gcgcctttcg
      361 ctttccaagc acgtgaacgc cgcgggcgag aagcgtgtct cggtctcgga cgcgggccag
      421 gaggccgaga ccatcttcca cctggtcgag cgcggcaccg agatgagcct cctcgaggcc
      481 gagctcctca ccgggcgcac gcaccagatc cgcgtgcacc tcgcgcacct ggggcacccg
      541 gtgctgggcg acgacaagta cggcgatttc gagctgaacc accgcctgcg caaggagggg
      601 ctgaagcgca tgttcctgca cgccgcgaag ctttccttcg cgcatccggt aacgggccaa
      661 agagtcgacc tcgtttcgcc gctacccgcg gatctcgaga aattccgctc gcgcattaaa
      721 gcggttgacg cagatgaacg cagatagagc gcagatgaac gtacttcgac gggtcgttct
      781 cggcccagcg atattcgacg tggaggtacg ttcatctgcg ctctatctgc gttcatctgc
      841 gtccatgcac tagccaggag caaccaggaa attgaactac gagctggtga tcttcgattg
      901 ggacggcacg ctgatggact cgacgagggt gatcgcatcg agcctgcaaa gcgcatgttc
      961 cgacgtgggc gtcccggtcc ccagcgagcg tgacgcgctt tatgtgatcg ggttgaatct
     1021 cgccgacacc ttcaaccacg tcgctcccgg tctcgacgag gagggtcgca ggcgcctttc
     1081 ggagcgctac cgccatcctt tcctcgcgcg cgagcacgag atgccgctct acgacggcgt
     1141 ggtggagatg ctctccgacc tgcacgggaa aggagcgcgc ctcgcggtag ccaccggcaa
     1201 ggcgcgtcgc ggcctggacc gcgtgctcga cgcgaccggc ctcggacgct ggttcgaggc
     1261 cacgcgctgc gcggacgagg gattcgcaaa gccacacccc ggaatgctgc tcatgctgat
     1321 ggacttgacc ggcgtcgagc cgcggcgcgc gctcatggtg ggagacacga ctcacgacct
     1381 ggaactcgcc gccaacgccg gcgtggacgc ggtcgcggtg acctacggcg cgcacgatga
     1441 gaagctgctc ggcacgcgcc cggccaaggc gcgctgcgca agcgtgccgg agctgcaccg
     1501 atggctga
//
LOCUS       DASVII010000401         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_485728,
            whole genome shotgun sequence.
ACCESSION   DASVII010000401 DASVII010000000
VERSION     DASVII010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485728"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..818
                     /locus_tag="VF309_05245"
     CDS             147..818
                     /locus_tag="VF309_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7466026.1"
                     /translation="MKIQSFLLTADHISTWAGKAAAWLVMVLMLVVCVEVFKRYLLNA
                     PTAWIFDITNMLYGTLFMMCGAYTLAQDAHVRGDFLYGSMKPRTQATLDLILYILFFL
                     PGMMALIYAGYDYAAISWHINEHSNVSSNGPAVYPFKTIIPIAGVLVLIQGIAEIMRC
                     IVCLKTGVWPDRLKDAEEIDVIEQQLAGSTLVDDESRKRAIEGAHEIDEAAHQRIGSR
                     GAERE"
     gene            811..>1614
                     /locus_tag="VF309_05250"
     CDS             811..>1614
                     /locus_tag="VF309_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7466027.1"
                     /translation="MSDPALGLLMLTLIVVVIMLGFPTAFTLMGLGMMFGFVAFYDPS
                     HSWADNKVFDLMVERTYGAMNNDVLISIPLFVLMGYVMERGALVDKMFYSIQLAFRRV
                     PASLAVATLIVCTFWGIASGLVGAVVVLMGVIAFNPMLKAGYDVRLASGVITAGGTLG
                     ILIPPSVMIIVYAAVAGQSVVKLYASAMFPGFFLSFLYLLYIIGWALLNPKIAPRLPD
                     SELKVPVPAWIPNFQQAYSKNMLVSLLTAVVRPARALAIDVDGRAMSYGG"
BASE COUNT          259 a          542 c          494 g          319 t
ORIGIN      
        1 gtacaaccac tacttcggca aggccgcccc tgcggcgaag aaggcctgac cgccggaagg
       61 caaccagggg caccatccgg gcttcgctcc cgggtggtgc ctcgtttttc gtagcggctc
      121 attttccgga agcgtaggcg cgccccatga agatccagag tttcctgctg accgccgacc
      181 acatcagcac gtgggccggc aaggccgccg cgtggctggt catggtgctg atgctggtcg
      241 tctgcgtgga ggtgttcaag cgctacctgc tgaatgcgcc gaccgcgtgg atcttcgaca
      301 tcacgaacat gctctacggc acgctcttca tgatgtgcgg cgcctacacc ctcgcgcagg
      361 acgcacacgt ccggggcgac ttcctctacg gctcgatgaa gccgcgcacc caggcgacac
      421 tcgacctcat cctctacatc ctcttcttcc tgcccgggat gatggcgctc atctacgcgg
      481 gatacgacta cgccgccatc tcgtggcaca tcaacgagca ttcgaacgtc tcgtccaacg
      541 ggcccgcggt atacccgttc aagaccatca tcccgatcgc cggcgtcctc gtcctcatcc
      601 aggggatcgc cgagatcatg cgctgcatcg tttgcctgaa gaccggcgtc tggccggatc
      661 gactgaagga cgccgaggag atcgatgtga tcgagcagca gctcgcgggc agcacgctcg
      721 tggacgacga atcgcgcaag cgcgcgatcg agggtgccca cgaaatcgac gaggctgcgc
      781 accagcgcat cggctcgagg ggagcggaac gtgagtgatc ccgcactcgg ccttctgatg
      841 ctcacgctga tcgtggtcgt gatcatgctg ggcttcccga ccgccttcac gctgatgggc
      901 ctgggcatga tgttcggctt cgtcgccttc tacgacccgt cgcactcctg ggccgacaac
      961 aaggtcttcg acctcatggt cgagcgcacc tacggcgcga tgaacaacga cgtgctgatc
     1021 tcgattccat tgttcgtgct catgggctac gtgatggaac gaggggcgct ggtggacaag
     1081 atgttctaca gcatccagct cgcgttccgg cgcgtgcccg cgtccctcgc ggtggcgacc
     1141 ctcatcgtgt gcacgttctg gggcatcgcg agcggcctgg tcggcgcggt cgtggtgctg
     1201 atgggcgtga tcgcgttcaa cccgatgctc aaggccggct acgacgtgcg cctcgcctcg
     1261 ggcgtcatca ccgcgggcgg cacgctcggc atcctcattc cgccgtcggt gatgatcatc
     1321 gtctacgcgg cggtcgccgg gcagtcggtg gtgaagctct acgcctcggc gatgttcccc
     1381 ggcttcttcc tgtcgttcct ctatctcctc tacatcatcg gctgggcgct cctcaatccc
     1441 aagatcgccc cgcgcttgcc cgactccgag ctgaaggtgc cggtccccgc ctggatcccg
     1501 aatttccagc aggcttactc gaagaacatg ctggtgagcc ttctcaccgc ggtggttcgg
     1561 cccgccaggg ccctcgcgat cgatgtcgac gggcgcgcga tgtcctacgg cggt
//
LOCUS       DASVII010000402         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_126517,
            whole genome shotgun sequence.
ACCESSION   DASVII010000402 DASVII010000000
VERSION     DASVII010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126517"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VF309_05255"
     CDS             complement(<1..625)
                     /locus_tag="VF309_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7466028.1"
                     /translation="MADAPMVTLTVEGREVQVPAGTSILEAVKLAGVLVPHYCYHPGL
                     PVAGVCRMCLVEVEKAPKLAPACATAVADGQVVHVHSEKSLKARQGVLELLLINHPLD
                     CPICDQAGECELQDYTFREGRTEGRLGTEPKRFNPVEDFGGDVLYVPNRCILCTRCVR
                     YMDEIVQEPVLNVSERGDRAVIGKFDGHDLTNEWASNVVDLCPVGALL"
     gene            complement(625..>1671)
                     /gene="nuoF"
                     /locus_tag="VF309_05260"
     CDS             complement(625..>1671)
                     /gene="nuoF"
                     /locus_tag="VF309_05260"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7466029.1"
                     /translation="DESEPGTFKDREIMRWTPHALVEGCAIGAYAIGAEIAYVYIRGE
                     YTEALEKMEAACREAYAAGILGTNAMGTGKTVHVYVHKGAGAYICGEETALMNSLEGR
                     RGNPRIKPPFPAVSGLFNQPTTINNVETLVAVPHILNKGADWYKKFGRADNPKSTGTK
                     LFSVCGNITFPGNYEVALGFPFKDFLYDLCGGPLPGRNFKAIIPGGISVPIQTREEAE
                     ATLMDYEGFVAQGSMLGSGGVIIFDDSQCMVRHIARLARFYAHESCAQCTQCREVTAW
                     TTKILERIEAGGGTMQDFDTLLEIADNMTGKTICVLSDSCAAPVVSGIQKFKHEFEAH
                     ITGKRCPFRATAAA"
BASE COUNT          274 a          566 c          523 g          308 t
ORIGIN      
        1 agaggagcgc gcccaccgga cagagatcga cgacgttcga cgcccactcg ttggtgagat
       61 cgtgtccgtc aaacttgccg atcacggcgc gatcgccgcg ctcgctcacg ttgagcaccg
      121 gctcctgcac gatctcgtcc atgtagcgca cgcagcgtgt gcagagaatg cagcggttcg
      181 ggacgtagag cacgtcgccg ccaaaatcct cgaccggatt gaagcgcttc ggctcggtgc
      241 cgagccggcc ttccgtacgc ccttcgcgga acgtgtagtc ctgcagctcg cattcgccgg
      301 cctgatcgca gatcgggcag tcgagcggat ggttgatgag caggagctcg agcacgccct
      361 gtcgcgcctt gagcgacttc tcggagtgca cgtgcaccac ctgcccgtcg gccaccgcgg
      421 tcgcgcacgc gggcgcgagc tttggcgcct tctccacctc gacgagacac atgcggcaca
      481 cgcccgcgac cggtagcccc gggtgatagc aatagtgggg aacgagaacg cccgcgagct
      541 tcacggcctc gaggatcgag gtgccggcgg gaacctggac ctcgcgtccc tccaccgtga
      601 gcgtgaccat cggcgcgtca gccatcaggc ggccgcggtc gcgcggaatg ggcagcgctt
      661 acccgtgatg tgcgcctcga actcgtgctt gaacttctga attcccgaga cgaccggtgc
      721 cgcgcacgaa tcgctcaaca cgcagatcgt cttccctgtc atgttatcgg cgatctcgag
      781 gagcgtgtcg aagtcctgca tcgtgccgcc gcccgcctcg atgcgctcca gaatcttggt
      841 cgtccacgcc gtaacctcgc ggcactgcgt gcactgcgcg caactctcgt gcgcgtagaa
      901 gcgcgcgagt cgcgcgatgt gccgcaccat gcactgcgag tcgtcgaaga tgatcacgcc
      961 gccggagccg agcatcgagc cctgcgccac gaatccctcg tagtccatca gcgtggcttc
     1021 tgcttcctcg cgcgtctgga tgggcacgga gatgccgccc gggatgatcg ccttgaagtt
     1081 cctccccggc agcggtccgc cgcagagatc gtacaggaaa tccttgaagg gaaatccgag
     1141 ggcgacttcg tagttgccgg gaaaggtgat gttgccgcag acggagaaca acttggtccc
     1201 cgtgctcttc ggattgtccg cccggccgaa cttcttgtac cagtccgcac ccttgttgag
     1261 aatgtgcggc accgccacca gcgtctcgac gttgttgatc gtcgttggct ggttgaacag
     1321 cccgctcacc gccgggaagg gcggcttgat gcgcggattg ccgcggcgtc cttcgagcga
     1381 gttcatgagc gcggtctctt cgccgcagat gtacgcgccc gcacccttgt gcacgtacac
     1441 gtgcaccgtc ttgccggtgc ccatcgcatt ggtgccgagg atgccggcgg catacgcctc
     1501 gcggcaggcc gcctccatct tctccagcgc ctcggtgtac tcgccgcgaa tgtacacgta
     1561 cgcgatctcc gcaccgatgg cgtacgcgcc gatcgcgcag ccttccacca gcgcgtgcgg
     1621 cgtccagcgc atgatctcgc gatctttgaa ggtgccgggc tccgattcgt c
//
LOCUS       DASVII010000403         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_378453,
            whole genome shotgun sequence.
ACCESSION   DASVII010000403 DASVII010000000
VERSION     DASVII010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378453"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /gene="rplB"
                     /locus_tag="VF309_05265"
     CDS             <1..792
                     /gene="rplB"
                     /locus_tag="VF309_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX7466030.1"
                     /translation="RFRSVSDFDVITRTTPEKSLLEPIKKSGGRDNHGHISMRRIGGG
                     HKRQYRIIDFKRNKFGVVGTVKEIEYDPNRTARIALIEYADGEKRYVLHCKGLSQGDT
                     IVSGPGSDVRTGNAMPLKEVPLGTDVHNVELIPGKGGQMARSAGTSVQVVAKEGEYVT
                     LRMQSTEMRMVHGNCMATIGEVGNSEHELLSAGKAGASRWAGKRPKVRGEVMNPVDHP
                     HGGRTRGGRNVVSPWGKKEGVKTRDKKKASQRLLVRGRKRGKATQ"
     gene            817..1071
                     /gene="rpsS"
                     /locus_tag="VF309_05270"
                     /pseudo
     CDS             817..1071
                     /gene="rpsS"
                     /locus_tag="VF309_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            1207..1566
                     /gene="rplV"
                     /locus_tag="VF309_05275"
     CDS             1207..1566
                     /gene="rplV"
                     /locus_tag="VF309_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX7466031.1"
                     /translation="MSEARAIQRTARQSPYKMRLVIDQVRGMNVNEALALLKFSKKHA
                     AKQIEKVLKSAVANAEQASRNANESIDVDALFVKKAIVNEGPPLKRFMPAAMGRATPI
                     RKRTSHIEIIVGEKEGR"
     gene            1566..>1967
                     /gene="rpsC"
                     /locus_tag="VF309_05280"
     CDS             1566..>1967
                     /gene="rpsC"
                     /locus_tag="VF309_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX7466032.1"
                     /translation="MGQKTNPIGFRLGVSKQWRSKWYAKRDFPALLKEDELLRKYLKA
                     RLASAAIADITIERKPRKVVVTIHTGRPGVVIGKKGAAVEELKNELQQLSGKEVGINV
                     EEIKRPEIEAQLVADNIAAQLAQRISFRRAMK"
BASE COUNT          435 a          613 c          656 g          263 t
ORIGIN      
        1 cgcttccgct cggtctcaga cttcgacgtc atcacgcgca ccacgcctga aaagtcgctg
       61 ctcgagccga tcaagaagag cggcggccgc gacaatcacg gtcacatctc gatgcggcgc
      121 atcggcggcg gtcacaagcg ccaataccgc atcatcgatt tcaagcgcaa caagttcggt
      181 gtcgtcggaa cggtgaagga gatcgagtac gatcccaacc gcaccgcccg catcgcgctc
      241 attgaatacg ccgacggcga gaagcgttac gtgctgcact gcaagggact ctcgcagggc
      301 gacacgatcg tttccggtcc gggctctgac gtccgcacgg gcaacgcgat gccgctgaag
      361 gaagttccgc tcggcaccga cgtgcacaac gtcgagctca ttccgggcaa gggcggccag
      421 atggcgcgct cggccggcac gagcgtgcag gtcgtcgcga aagaaggcga gtacgtcacg
      481 ctgcgcatgc agagcacgga aatgcgcatg gtgcacggca actgcatggc gacgatcggc
      541 gaagtcggaa actccgagca cgagcttctg agcgcaggca aggcgggcgc gtcgcgctgg
      601 gccggcaagc gtccgaaggt gcgcggcgaa gtgatgaacc cggtggacca cccgcacggt
      661 ggccgcacac gcggcggccg caacgtggtg agcccgtggg gcaagaagga aggcgtcaag
      721 acgcgcgaca agaagaaagc ctcgcagcgg ttgttggtgc gcggccggaa gcgcggcaaa
      781 gccacgcagt aacgcgatat ctaaggactc gagactatgg ccagaagcat tcgaaagggg
      841 cccttcgttc aggacagcct gctgaagcgc gtgcagggac tgaactcgag gagcgagaag
      901 aaggtcgtca agacctggtc gcgcgcgagc accgtgcttc ccgatttcgt cgggcacacg
      961 ttcgccgtgc acaacgggaa caagttcatc ccggtgtacg tgaccgaaaa catggtcggg
     1021 cacaagctcg gcgagttcgc gccgacgcgc ctcttccgcg gtcacaccgc ggcggggtcg
     1081 aaggccgcgg cagaagcgaa ggcgacggac cagaaggcgc gcccaggcgc accggctggc
     1141 gccgccgcac ccgctgctgc tgcagcgccc gccgccgcac ccgccggcaa gggaggcaag
     1201 taacccatgt ctgaagcccg cgcgatccag cgcactgcgc gccagtcgcc gtacaaaatg
     1261 cggctggtga tcgatcaggt gcgcggcatg aacgtgaacg aggcgctcgc gctcctcaag
     1321 ttttccaaga agcacgcggc gaagcagatc gagaaggtgc tcaagagcgc tgtcgcgaac
     1381 gccgagcagg cgtcgcgcaa cgcgaacgag tcgatcgatg tcgacgcgct gttcgtgaag
     1441 aaggcgatcg tcaacgaagg cccgccgctc aagcgcttca tgccggcggc gatgggacgc
     1501 gccacaccaa tccgcaaacg caccagccac atcgagatca tcgtgggcga gaaggaaggt
     1561 cgataatggg acagaaaacc aatccgatcg gtttccgcct cggcgtctcc aagcagtggc
     1621 gctcgaagtg gtacgccaag cgtgactttc cggcgctgct caaggaagac gagctgctcc
     1681 gcaaatatct gaaggcgcgc ctcgcgagcg cggcgatcgc cgacatcacc atcgaacgga
     1741 agccgaggaa ggtcgtcgtg accatccaca cgggccgtcc gggcgtggtg atcggcaaga
     1801 agggcgccgc ggtcgaggaa ctgaagaacg agctccagca gctgagcggc aaggaagtcg
     1861 gcatcaacgt cgaggaaatc aagcgcccgg aaatcgaagc gcagctcgtc gccgacaaca
     1921 ttgccgcgca gctcgcgcag cgcatttcgt tccgccgcgc gatgaag
//
LOCUS       DASVII010000404         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_198003,
            whole genome shotgun sequence.
ACCESSION   DASVII010000404 DASVII010000000
VERSION     DASVII010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198003"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VF309_05285"
     CDS             <1..498
                     /locus_tag="VF309_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEX7466033.1"
                     /translation="IKRSNTLVIFINQIRMKIGVMFGNPETTTGGNALKFYASVRIDI
                     RRIGSIKRGEEVIGNETRVKVVKNKVAPPFKTADFDILYNEGISREGEIIEMGVNHRI
                     VEKSGAWYAYKGEKIGQGKDNTREFLRENPAMANEIEMKIREAVGIAGGMPQAKVEAE
                     AAEGD"
     gene            534..995
                     /gene="recX"
                     /locus_tag="VF309_05290"
     CDS             534..995
                     /gene="recX"
                     /locus_tag="VF309_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEX7466034.1"
                     /translation="MAWAFVAKTEPSLRARAMRFLARREHSRAELQRKLSAVAEEGDD
                     VEALLDELASKGWLSDTRFAELAVRSKARRFGPLKVAHQLRAKGVGEETIAIAFASAG
                     TDGAANLERVWRSHFSAAPADQRERGRQVRFLQGRGFPLDDILRFLKGMRT"
     gene            992..1381
                     /locus_tag="VF309_05295"
     CDS             992..1381
                     /locus_tag="VF309_05295"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX7466035.1"
                     /translation="MRVTGMFDVKLAPQASDDVGDGIPLGRMSIEKRYHGDLDATGKG
                     EMLTAMTSVKGSAGYVAMERVSGALQGRKGSFVIQHGGTMTRGAQSLSITVVPDSGRG
                     ELEGIAGKMAIVIADGKHSYDFEYTLS"
     gene            1406..1528
                     /locus_tag="VF309_05300"
     CDS             1406..1528
                     /locus_tag="VF309_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466036.1"
                     /translation="MSWKQQKILSLAAIAAAFVINCWPVQSQGPAPVHTVLAGP"
BASE COUNT          313 a          512 c          554 g          246 t
ORIGIN      
        1 atcaagcgct ccaacacgct cgtcatcttc atcaaccaga tccgcatgaa gatcggcgtg
       61 atgttcggca accccgagac caccaccggc ggcaacgcgc tcaagttcta cgcgtcggtg
      121 cgcatcgaca tccgccgcat cggctcgatc aagcgcggcg aagaggtgat cggcaacgag
      181 acgcgcgtga aggtcgtcaa gaacaaggtg gcgcccccgt tcaagaccgc ggacttcgac
      241 atcctctaca acgagggcat ctcgcgcgag ggcgagatca tcgagatggg cgtcaaccac
      301 cgcatcgtcg agaagtccgg cgcctggtac gcctacaagg gcgagaagat cggccagggc
      361 aaggacaaca cgcgcgagtt cctgcgagag aaccccgcca tggccaacga gatcgagatg
      421 aagattcgcg aggccgtggg catcgcgggc ggcatgcccc aggccaaggt cgaagcggaa
      481 gcggctgaag gcgactgaag gccggcgcgg cgaattcgct gaggtggtga cccatggcct
      541 gggcattcgt ggctaagacc gagccgtcat tacgggcccg ggccatgcgg tttcttgcgc
      601 ggcgcgagca ttcgcgcgcc gagctccagc gaaagctttc cgcggtggcg gaggaaggcg
      661 acgatgtcga ggcattgctc gacgagctgg cgagcaaagg ctggctctcg gacacgcgct
      721 tcgcggagct cgcggtgcgc tccaaggcgc gccggttcgg cccgctcaag gtcgcccacc
      781 agttgcgcgc caagggcgtg ggtgaagaga ccatcgcgat tgcctttgca agcgcgggca
      841 ccgacggcgc cgcgaacctc gagcgggtgt ggcgcagcca cttctcggcc gcccccgccg
      901 atcaacgtga gcggggccgc caggtgcggt tcctccaggg acgcggtttc ccgctcgacg
      961 acatcctgcg cttcctgaag ggaatgagga catgagagtc accggaatgt tcgacgtgaa
     1021 gctggcgccg caagcgagcg acgatgtcgg cgatggcatc ccgctgggcc gcatgtcgat
     1081 cgagaagcgc taccacggcg atctcgatgc gaccggcaag ggcgagatgc tcacggcgat
     1141 gacgtcggtg aagggctccg cgggatacgt cgccatggag agggtcagcg gcgcgttgca
     1201 gggccgcaag ggatccttcg tgatccagca tggcggcacg atgacgcgcg gcgcgcagag
     1261 cctttcgata accgtcgtcc ccgactccgg caggggagag ctggagggca tcgccggaaa
     1321 gatggccatc gtgatcgccg acggcaagca ttcatacgat ttcgaataca cgctctcctg
     1381 acctttactg gaggaaatcc gcgccatgag ctggaagcaa cagaagatcc tctccctcgc
     1441 cgccatcgcg gcggcgttcg tcatcaactg ctggcccgtg caatctcagg ggcccgctcc
     1501 cgtgcacacg gtcctggcgg gtccgtgacg atctcccagg ccgagaaggc cgcgcgcttc
     1561 aaggcgcttc acgagggtcc gcgcgcgttc gtcatcgcca atccgtggga tgggggctcg
     1621 gcgcg
//
LOCUS       DASVII010000405         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_306236,
            whole genome shotgun sequence.
ACCESSION   DASVII010000405 DASVII010000000
VERSION     DASVII010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306236"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VF309_05305"
     CDS             <1..239
                     /locus_tag="VF309_05305"
                     /inference="COORDINATES: protein motif:HMM:NF041730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase H-associated membrane protein"
                     /protein_id="HEX7466037.1"
                     /translation="AIGVASEVAVQLGALAGPTGAPLIAAGAVSRTLAALGFQLGTLI
                     FPSVVPMMLWVAMDTRLVQRIGTSLRARREEIAA"
     gene            complement(241..1374)
                     /locus_tag="VF309_05310"
     CDS             complement(241..1374)
                     /locus_tag="VF309_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7466038.1"
                     /translation="MSVLSGVKIVEVGGIGPAPFCGMLLADLGAEVILIERPEAGRMT
                     DMGAAAIFNRGKRSIALDLKSQRGAETALRLIDGADGLIEGMRPGVMERLGLGPDACL
                     ARNPRLVYGRMTGWGQSGPLANAAGHDNNYLALSGALWYAGSGGEPPVAPPTLLGDLG
                     GGALYLAVGVLAGILSARATGVGTVVDAAIVDGSAHLMNLLLAFRASGQFSMQRGKSL
                     LDGPHWFATYRCSDGNFITVGALEPQFYGLLLRKLGLDADPAFADGYDASRWPELKQR
                     FAELFATRDRDAWCALLEGTDACFAPVLTPEEAARHPHIVDRGIYGEREGMLQANPAP
                     RFSGPRAVPGPVPRRGEHADEILRAAGLSTAEIEALRAGGVIA"
     gene            complement(1388..>1611)
                     /locus_tag="VF309_05315"
     CDS             complement(1388..>1611)
                     /locus_tag="VF309_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466039.1"
                     /translation="SISVSTPGTDPTDDFSCFAMFIATGSFFLGQAKLLPESMRIFPL
                     LAVPVVLVLLLMVYWWVRVSFTKRIPQRV"
BASE COUNT          268 a          550 c          555 g          238 t
ORIGIN      
        1 ccgcgatcgg cgtggcgagc gaggtcgcgg tgcagctcgg cgcgctcgcg ggcccgacgg
       61 gagcgccgct catcgcggcg ggcgccgtgt cgagaaccct cgcggcgctc ggcttccagc
      121 tgggcacgct catctttccc agcgtcgtgc ccatgatgct atgggtggcg atggacacgc
      181 ggctcgttca acgcatcggc acgagcctgc gggcacgccg cgaagagatc gccgcgtagc
      241 tcaggcgatc acgccgccgg cgcgcaacgc ttcgatctcg gccgtggaca atccggcggc
      301 gcgaagtatc tcgtcggcat gctcgcctcg cctgggaacc gggccgggaa cggcacgcgg
      361 gccggagaat cgcggcgccg gattggcctg cagcattcct tcgcgctcgc cgtagattcc
      421 gcgatcgacg atgtgcggat ggcgcgcggc ttcctccggc gtgagcaccg gcgcgaagca
      481 cgcatcggtt ccttcgagga gcgcgcacca cgcgtcgcga tcgcgagtgg cgaagagctc
      541 cgcgaagcgt tgcttcagct cgggccagcg gctcgcgtcg tagccgtcgg cgaacgcggg
      601 atcggcgtcg aggccgagct tgcgcaggag cagcccgtag aactgcggct cgagcgcgcc
      661 cacggtgatg aagttgccgt cggagcagcg ataggtcgcg aaccagtgcg gaccgtccag
      721 caggctcttg ccgcgctgca tcgagaattg gccgctggcc ctgaatgcga gcaacaggtt
      781 catgaggtgc gcggagccgt cgacgatggc cgcatccacg accgttccga caccggttgc
      841 ccgtgcgctc agtattcccg cgagaacgcc gaccgcgaga tagagcgcgc cgccgccgag
      901 atccccgagc agcgtggggg gcgctacggg aggctcgccg ccgctgccgg cataccagag
      961 ggcgccggat agcgcgaggt agttgttgtc gtggccggcg gcgttcgcca gcggcccgct
     1021 ctgtccccaa ccggtcatgc gtccgtacac gagcctgggg ttgcgcgcga ggcacgcgtc
     1081 cggacccagc ccgaggcgct ccatgacgcc cggacgcatg ccctcgatga ggccgtcggc
     1141 gccgtcgatc aggcgcagcg ccgtttccgc gccgcgctgc gatttcaggt cgagcgcgat
     1201 cgagcgcttg ccgcgattga agatcgccgc ggcacccatg tcggtcatgc ggcccgcttc
     1261 cggacgctcg atgaggatca cctcggcgcc aagatcggcg agcagcattc cgcagaaggg
     1321 cgcgggaccg attccgccga cctccacgat cttcacgcct gacagcacgc tcatcgggct
     1381 tcctgcctca cactcgttgc gggatcctct tcgtgaacga cacgcgcacc caccagtaaa
     1441 ccatgagcag caggaccagc acgaccggga ctgcgagcaa gggaaagatg cgcatcgact
     1501 ccggcaacaa cttggcctgc cccaggaaga acgaaccggt cgcgatgaac atcgcaaagc
     1561 acgagaagtc gtccgttggg tcggttcccg gcgtcgaaac cgaaattgat c
//
LOCUS       DASVII010000406         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_485968,
            whole genome shotgun sequence.
ACCESSION   DASVII010000406 DASVII010000000
VERSION     DASVII010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485968"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VF309_05320"
     CDS             complement(<1..507)
                     /locus_tag="VF309_05320"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEX7466040.1"
                     /translation="MKGPQEAMRLLAEAEELFAADAVRAAVERVGAEISAKLKDEFPV
                     VLSVMGGAAIFTGQLLPLLPFPLEFGAIEVTRYNNDIEGREVTWRLPPRDNVRGRVVL
                     VLDDILDEGITLAAIHKRLLEMGAREVYSAVFADKAIGRPKPIAADFVGVTVPNRYVF
                     GFGMDAYGL"
     gene            complement(504..1388)
                     /gene="galU"
                     /locus_tag="VF309_05325"
     CDS             complement(504..1388)
                     /gene="galU"
                     /locus_tag="VF309_05325"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX7466041.1"
                     /translation="MPKVTKAVFPVAGLGTRFLPATKASPKEMMPIVDKPLIQYAVEE
                     AVAAGITDLIFITGRGKRAIEDHFDKAYELEAELQAHGKTKLLKEVRGLLPEGVSCAY
                     VRQAEALGLGHAVLCARHLVGDEPFAVILADDLIDAQVPVMKQMSQVHAKSGGSVIAV
                     QNVAREDTGSYGIVSSDKVSPGLSRITNIVEKPKPDDAPSTLAVVGRYILSPRIFHFL
                     ENLRPGAGREIQLTDAIGRLLREESVMAYEFQGQRYDCGSKLGYLKATVNYALKHPEV
                     ADDFRAFLGIAGAAVKKT"
     gene            complement(1393..>1859)
                     /locus_tag="VF309_05330"
     CDS             complement(1393..>1859)
                     /locus_tag="VF309_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7466042.1"
                     /translation="IRNVGEVTGKDLARHFGTLDALMDASLEELQEAPDVGPIVAQSI
                     ASFFREAHNREVIEQLRAAGVRWKEGAPPKKASAGPFTGKIVVLTGTLAAMSRDEAKD
                     RIESLGGRVTGSVSKKTDYVVAGDEAGSKLDRARSLGVAVLDEDEFLKLLGE"
BASE COUNT          273 a          647 c          616 g          323 t
ORIGIN      
        1 gaggccgtag gcgtccatgc cgaagccgaa gacgtagcgg ttcggaaccg tcacgccgac
       61 gaaatcggcg gcgatgggtt tcgggcgccc gatcgccttg tcggcgaaga cggcgctgta
      121 gacctcgcga gcgcccatct cgaggagcct cttgtggatc gccgcgagcg taatgccctc
      181 gtcgaggatg tcgtcgagca ccagcaccac ccggccgcgc acgttgtcgc gcggcggaag
      241 ccgccacgtg acctcgcggc cctcgatgtc gttgttgtag cgcgtgacct cgatcgcgcc
      301 gaactcgagc gggaacggca gcagcggcag gagctgcccg gtgaagatcg ccgctccgcc
      361 catcaccgaa agcaccaccg gaaattcgtc cttcagcttc gccgagatct cggcgcccac
      421 gcgctccacc gccgcgcgca ccgcgtcggc cgcgaagagc tcctcggcct cggcgaggag
      481 gcgcatcgcc tcctggggtc ccttcacgtc ttcttcaccg cggcgccggc gatgccgagg
      541 aacgcgcgga aatcgtcggc gacctccgga tgcttcagcg catagttcac cgtcgccttc
      601 aggtagccca gcttcgaccc gcagtcgtag cgctgcccct ggaattcgta cgccatcacg
      661 ctctcctcgc gaagcaggcg cccgatggcg tcggtcagct ggatctcgcg gcccgcgccg
      721 ggacgcaggt tctcgaggaa atggaagatg cgcggcgaga ggatgtagcg gcccacgacc
      781 gccagcgtcg agggcgcgtc gtcgggcttg ggcttttcga cgatgttggt gatgcgcgaa
      841 aggcccggcg acaccttgtc cgaggacacg atgccgtagc tcccggtgtc ctcgcgcgcc
      901 acgttctgca cggcgatcac gctgccgccg gatttcgcgt gcacctgcga catctgcttc
      961 atcaccggga cctgcgcgtc gatgagatcg tcggcgagga tgacggcgaa cggctcgtcg
     1021 cccaccaggt ggcgcgcgca cagcaccgcg tggccgaggc cgagcgcttc ggcctggcgc
     1081 acgtaggcgc agctcacgcc ctcgggaagg agcccgcgca cttccttcag gagcttggtc
     1141 ttgccgtgcg cctgcagctc ggcttcgagc tcgtaggcct tgtcgaaatg atcctcgatg
     1201 gcgcgcttgc cgcggcccgt gatgaagatg aggtcggtga tgcccgcggc gaccgcctcc
     1261 tcgaccgcgt actggatgag cggcttgtcg acgatcggca tcatctcctt cggcgaggcc
     1321 ttcgtcgccg ggaggaagcg cgtgccgagg cccgcgacgg ggaagacggc tttggtgacc
     1381 tttggcattt tcctattcgc ccagcagctt gagaaattcg tcttcgtcga gcaccgcgac
     1441 cccgaggctg cgcgcgcggt cgagcttcga tccggcctcg tcgccggcga ccacgtagtc
     1501 ggtcttcttc gacaccgacc cggtcaccct gccgccgagg gattcgatcc tgtccttcgc
     1561 ctcgtcgcgc gacatcgccg ccagcgttcc ggtgaggacc acgattttac ccgtgaaggg
     1621 gccggcgctg gccttcttcg gcggcgcgcc ctctttccag cgcactccgg cggcgcgcag
     1681 ctgctcgatc acttcgcggt tgtgggcctc gcggaagaag ctcgcgatcg actgcgcgac
     1741 gatcgggccc acgtcgggcg cttcctggag ctcctcgagg ctcgcgtcca tgagcgcgtc
     1801 cagcgtgccg aaatggcgcg cgaggtcctt gcccgtgact tcgcccacgt tgcgaatgc
//
LOCUS       DASVII010000407         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_19160, whole
            genome shotgun sequence.
ACCESSION   DASVII010000407 DASVII010000000
VERSION     DASVII010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19160"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..867)
                     /locus_tag="VF309_05335"
     CDS             complement(88..867)
                     /locus_tag="VF309_05335"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7466043.1"
                     /translation="MRLADKVAIVTGAAQGIGLACARAFAHEGARVLLTDVNEDAGRG
                     ETLRLRSDGFKASFARCDVSRRIEVDAAIAAAVNEFGRVDILVANAGIVHAAEFLDLA
                     EADFDRVIAVNLKGIFLAGQAAARQMVKQGGGGAIINMSSVNAVLAIPNQVPYVVSKG
                     AINQLTKVMALSLAAHGIRVNGIGPGTILTELAKTAVLGNREAERKILSRTPMGRMGE
                     PDEIAQVAVFLASGAASYMTGQTLYPDGGRLALNYTVPVPD"
     gene            complement(872..1303)
                     /locus_tag="VF309_05340"
     CDS             complement(872..1303)
                     /locus_tag="VF309_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466044.1"
                     /translation="MSFLRVALASAGLALGAASIACAADKPCTKADAVNGEKAIERAN
                     NWSQLYKTWQDYRHCDTGAVADGYTDSLMRLMVEWKSVDALAAAMEKDPAFKEWIYAR
                     LKSPAAKDDQPAVYSRAMASCPKAMDAFCAELAESVKPAAK"
     gene            complement(1305..3197)
                     /gene="dxs"
                     /locus_tag="VF309_05345"
     CDS             complement(1305..3197)
                     /gene="dxs"
                     /locus_tag="VF309_05345"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7466045.1"
                     /translation="MSSYPLLETVDDPALLRTLDRKQLAQLATELRSFIVDSVSSTGG
                     HLSSNLGTVELTIALHYVFNTPDDKLIWDVGHQTYGHKILTGRRKGMRTLRQKGGIAG
                     FPRREESRYDTFGTAHSSTSISAAIGMAVAMRKRGLDNRAVAIIGDGAMTAGMAFEAL
                     NNAEQSGANVIVILNDNDMSISENVGALNNYLAKLMSGRFYAATKKTAEKVLSATPTV
                     KDFAKRFEEHAKGMLTPSTMFEEFGFNYTGPIDGHNIDVLIDTLQNVTRLKGPQFLHV
                     VTRKGQGYAYAEEDPITYHGPGKFDPGVGIVKVKPPIDARPKPTYTQVFGDWLCDMAR
                     LDDRLIGITPAMREGSGLVKFSQRFPERYFDVGIAEQHAVTLAAGMACDGLKPVVAIY
                     STFLQRGYDQLIHDVALQNLPVVFALDRGGLVGADGPTHHGAFDLSYLRCIPNMTVMA
                     PADENECRQMLYTAFTLDTPTAVRYPRGAGVGVEVQREMHAIPVGRGEVRREGKRIAI
                     LAFGSMLKPAMDAGAELNATVANMRFVKPIDRDLVFKLATSHDLLVTVEENVTAGGAG
                     AAVLEALAADGLAVPVLTLGLPAEFVEHGDPHQLLVDCGLDAEGIARSVRERLARAGQ
                     RSGKAA"
     gene            complement(3213..4109)
                     /locus_tag="VF309_05350"
     CDS             complement(3213..4109)
                     /locus_tag="VF309_05350"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX7466046.1"
                     /translation="MSADEFQSWSRDVAILLEETLGGLLPPARIVPSRLHDAMRYSTL
                     NGGKRVRAMLVFGAGEVTNADLKRLQIVASAVEMIHAYSLIHDDLPCMDDDILRRGKP
                     TCHVEFDEATALLAGDALQSLAFQILAENQLTENPATQLKMMQLFSAACGSRGMAGGQ
                     AIDLDSVGKTISLPELEFMHILKTGALIRASVIMGAQCGAELSQSEQDHLDRYAKLVG
                     LAFQVVDDILDEEGDSETLGKTAGKDKADNKPTYTSLMGLAEARRFALELLADANGAL
                     GDFGPRARRLADIADFIVHRRR"
BASE COUNT          635 a         1370 c         1447 g          726 t
ORIGIN      
        1 ggtcgcgcag gcgaggagcg ctgctgcaag gatgatccgc ttcatgggtg gacagctcct
       61 gggattgatc gcgggcggta gtgtacttca gtccgggacg ggcacggtgt agttcagcgc
      121 gaggcgcccg ccgtcggggt aaagggtctg gccggtcatg tacgacgccg cgccgctcgc
      181 gaggaacacc gcgacctggg cgatctcgtc gggctcgccc atgcggccca tgggcgtgcg
      241 cgaaaggatc ttcctctccg cttcgcggtt gccgagcacc gcggtcttcg cgagctcggt
      301 gaggatggtc ccggggccaa tgccgttcac gcggatgccg tgcgccgcga gcgacaacgc
      361 catcaccttg gtgagctggt tgatcgcgcc cttggagacc acgtagggaa cctggttcgg
      421 aatcgcgagg accgcgttca cgctcgacat gttgatgatc gcgcccccgc cgccctgctt
      481 caccatctgg cgcgccgccg cctgcccggc gaggaagatc cccttgaggt tcaccgcgat
      541 cacgcgatcg aaatccgcct cggccaggtc gaggaactcg gcggcgtgga cgatcccggc
      601 attggccacc aggatgtcga cgcggccgaa ctcgttgacc gccgcggcga tcgcggcatc
      661 gacctcgatc ctgcgcgaga cgtcgcagcg cgcgaagctc gccttgaagc cgtccgagcg
      721 caatcgcagc gtctcgccgc ggccggcgtc ctcgttcacg tccgtgagca gcacccgtgc
      781 gccctcgtgc gcgaaagccc gcgcgcacgc aaggccgatg ccctgcgccg caccggtgac
      841 gatcgcaacc ttgtcggcaa gcctcacgaa gctatttcgc ggcgggcttc acggattcgg
      901 cgagctcggc gcagaaagca tccatcgcct tcggacagct cgccatcgcg cgcgaataga
      961 cggccggctg gtcgtccttc gcggcggggc tcttcagccg ggcgtagatc cactccttga
     1021 acgcgggatc cttctccatc gccgcggcaa gcgcgtccac gctcttccat tcgaccatga
     1081 ggcgcatcag agaatcggtg tacccgtcgg cgacggcgcc cgtgtcgcaa tggcgatagt
     1141 cctgccacgt cttgtagagc tggctccagt tgttggcgcg ctcgatcgcc ttctcgccgt
     1201 tcaccgcgtc ggccttggtg cagggcttgt cggccgcgca ggcaatggat gccgcgccca
     1261 acgcgaggcc tgccgatgcg agcgcgacgc gcaggaagct catgtcacgc cgcctttccg
     1321 gagcgctgtc ccgcgcgcgc gagccgctcg cgcacgctgc gcgcgatgcc ctcggcatcc
     1381 agcccgcaat cgaccaggag ctggtgcgga tcgccatgct cgacgaactc cgcgggaagc
     1441 ccgagcgtca gcaccggcac cgcgagcccg tcggcggcga gcgcttcgag caccgcggcg
     1501 cccgcgccgc ccgcggtcac gttctcctcg accgtgacca gcaggtcgtg ggaagtcgcg
     1561 agcttgaaca cgaggtcgcg atcgatcggc ttcacgaagc gcatgttggc gaccgtcgcg
     1621 ttcagctccg cgcccgcgtc catcgcgggc ttcagcatcg atccgaacgc gaggatcgcg
     1681 atgcgtttgc cctcgcgccg cacttcgccc cgcccgacgg gaatggcgtg catctcgcgc
     1741 tgcacctcga cgccgacgcc ggcgccgcgc ggatatcgaa ccgcggtggg cgtatcgagc
     1801 gtgaacgcgg tgtagagcat ctggcggcac tcgttctcgt cggcgggcgc catcacggtc
     1861 atgttcggga tgcagcgcag gtacgagagg tcgaacgcgc cgtggtgcgt cgggccatcg
     1921 gctccgacca ggccaccacg gtcgagcgcg aagacgacgg gaaggttctg cagcgcgacg
     1981 tcgtggatga gctggtcgta gccgcgttgc aggaacgtgg aatagatcgc gaccaccggc
     2041 ttcaggccgt cacaggccat gccggcggcg agcgtcaccg cgtgctgctc cgcgatgccg
     2101 acgtcgaagt agcgctccgg gaagcgctgc gagaacttca ccaggcccga gccctcgcgc
     2161 atcgcgggcg tgatgccgat gaggcgatcg tcgagccgcg ccatatcgca gagccaatcg
     2221 ccgaacacct gcgtgtaggt gggcttgggc cgcgcgtcga tcggcggctt caccttgacg
     2281 atgcccacgc ccggatcgaa cttgccgggg ccgtggtagg tgatgggatc ttcctcggcg
     2341 taggcgtagc cctggccctt gcgcgtcacc acgtgcagga actgcgggcc cttgaggcgc
     2401 gtcacgttct gcagcgtgtc gatgaggacg tcgatgttgt ggccgtcgat ggggccggtg
     2461 tagttgaagc cgaactcctc gaacatcgtg gacggcgtga gcatgccctt ggcgtgctcc
     2521 tcgaagcgct tggcgaagtc cttcacggtg ggcgtggccg aaagcacctt ctccgcggtc
     2581 ttcttggtgg cggcgtagaa gcgccccgac atgagcttgg cgaggtagtt gttgagcgcg
     2641 ccgacgttct ccgagatcga catgtcgttg tcgttcagga tcacgatcac gttggcgccc
     2701 gattgctcgg cgttgttgag cgcctcgaac gccatgccgg ccgtcatcgc gccatcgccg
     2761 ataatcgcga ccgctcggtt gtcgaggccg cgtttgcgca tcgccaccgc catgccgatc
     2821 gccgcggaga tcgaggtcga gctatgcgcg gtgccgaacg tgtcgtagcg gctttcctcg
     2881 cgccgcggga aacccgcgat gccgcccttc tggcgcagcg tcctcatgcc cttgcgccgt
     2941 cccgtgagga tcttgtggcc gtaggtctgg tggcccacgt cccagatgag cttgtcgtcg
     3001 ggcgtgttga agacgtagtg cagcgcgatg gtgagctcga ccgtgcccag gttggacgag
     3061 aggtggccgc cggtggagga gaccgagtcg acgatgaagg agcgaagctc cgtggcgagc
     3121 tgcgcgagct gcttgcggtc cagggtccgc agcagcgcgg ggtcgtcgac cgtctcgagg
     3181 agggggtatg agctcatgcg agggtttcca ttctaacgcc gccggtggac gatgaaatcc
     3241 gcgatgtcgg ccaggcgccg ggcgcggggt ccaaagtccc ctagcgcacc attggcgtcg
     3301 gccaggagct cgagcgcgaa gcggcgcgcc tcggcgaggc ccatgaggct ggtgtaggtc
     3361 ggcttgttgt ccgctttgtc cttgcccgcc gtcttgccga gggtttccga atcgccttcc
     3421 tcgtccagga tgtcgtcgac cacttgaaaa gcgaggccca cgagcttggc gtagcggtcg
     3481 aggtgatcct gctcggattg cgagagctcc gcgccgcatt gcgcgcccat gatcaccgac
     3541 gcgcggatca gcgccccggt cttgaggata tgcatgaatt cgagctcggg gagcgagatc
     3601 gtcttgccga cggaatcgag gtcgatcgcc tgcccgcccg ccatgccgcg cgagccgcac
     3661 gccgcggaga agagctgcat catcttcagc tgcgtggcgg gattttccgt gagctggttc
     3721 tcggcgagga tctggaacgc gagggattgc agggcgtcgc cggcgaggag cgcggtggcc
     3781 tcgtcgaact ccacgtggca ggtgggcttg ccgcggcgaa ggatgtcgtc atccatgcac
     3841 ggcagatcgt cgtggatgag cgagtacgcg tggatcatct cgaccgcgct cgccacgatt
     3901 tgcaggcgct tcagatcggc attggtcact tcgccggcgc cgaacaccag catcgcgcgc
     3961 acgcgcttgc ctccgttgag cgtggaatag cgcatcgcgt cgtgcagccg cgaagggacg
     4021 atgcgcgccg gaggaaggag cccgccgagc gtctcctcca ggaggatcgc cacgtcgcgc
     4081 gaccacgact ggaattcgtc cgcgctcact tcgcgtcggg cgtgaagtcg cgcaacgtgt
     4141 ctccgtcgag gatcgcgatg cgcgcctggg cgtcggtc
//
LOCUS       DASVII010000408         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_485986,
            whole genome shotgun sequence.
ACCESSION   DASVII010000408 DASVII010000000
VERSION     DASVII010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485986"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="VF309_05355"
     CDS             <1..1630
                     /locus_tag="VF309_05355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7466047.1"
                     /translation="VAALPLTQEQREAIAQCSAKSDGFLAVLLQGVTGSGKTEVYLHL
                     IAKALAAGRQALFLVPEIGLTPQFEAQVRERFPDANLVAAHSHLNEGERAAAWLASQS
                     GAAHIVLGTRLAVLMPFRRLGLIVVDEEHDLSYKQQEGLRYSARDIAVRRAQRLGIPV
                     ILGSATPSLESFANAREGRYGLASLAKRAASGSAMPAVRTVDTRADRPREGFSHALVE
                     AIRGRLARKEQSLIFVNRRGFSPVLFCRACAWHSTCARCSANLVVHLGAREMRCHHCG
                     HRSRIPAVCPSCGAADLAPIGHGTQRVEDTLLALFPEARIARVDRDSTSRKGSLRGLL
                     EKVRKGEIDILVGTQMLAKGHDYPKLTLVGVLDCDSALFSADFRASERLFSQLVQVSG
                     RAGRGDAPGEVLIQTDFPAHPLFAAVARQDYARFAEDALEERRLAGFPPFSHLALLRA
                     ESRKAGEATVFLKLAARLAKRLASRREAKDIEVFDPVPAPLERKAGYERAQLLVRAAS
                     RATLQPFLHEWRLQLVDKGGRHIRWSLDVDPQEV"
BASE COUNT          236 a          619 c          578 g          256 t
ORIGIN      
        1 cgtggcggcc ctgccgctca cgcaggaaca gcgcgaggcg atcgcgcagt gctccgcgaa
       61 atccgacggc ttcctcgccg tgctccttca aggcgtgacc ggaagcggca agaccgaggt
      121 gtacctgcac ctcatagcga aagcgctcgc cgcgggacgc caggcgctct tcctcgttcc
      181 cgagatcgga ctcacgccgc agttcgaggc gcaggtccgc gagcgcttcc ccgacgcgaa
      241 cctggtggcg gcccacagcc acctcaacga aggcgagcgc gccgccgctt ggctcgcatc
      301 gcaatcgggc gcggcgcaca tcgtgctcgg gacgcgcctc gcggtgctga tgccgttccg
      361 ccgcctcggg ctcatcgtgg tcgacgagga gcacgacctg tcgtacaagc agcaggaggg
      421 actgcgctac tcggcgcgcg acatcgcggt acgccgcgcg cagcgcctcg gaattcccgt
      481 gatcctcggc tcggccacgc cgtcgctcga aagcttcgcc aacgcgcgcg aaggccgcta
      541 cggcctcgca tccctcgcga agcgcgccgc gagcgggtcg gcgatgccgg ccgtgcgcac
      601 cgtcgatacc cgcgccgatc gcccccgcga aggcttcagc cacgcgctgg tggaggcgat
      661 tcgcgggcgc ctcgcgcgga aggagcagtc cctcatcttc gtgaaccgcc gcggcttctc
      721 gccggtgctc ttctgccgcg cctgcgcgtg gcactcgacg tgcgcgcggt gcagcgcgaa
      781 cctcgtcgtg cacctgggag cgcgcgagat gcgttgccat cattgcgggc atcgcagccg
      841 catcccggcc gtgtgcccaa gctgcggggc cgcggatctc gctcccatcg gccacggcac
      901 gcagcgcgtc gaagacaccc tgctcgcgct attccccgag gcgcgcatcg cgcgcgtgga
      961 tcgcgactcg acctcgcgca agggctcgct gcggggcctc ctcgagaagg tccgcaaggg
     1021 cgagatcgac atcctcgtgg gcacgcagat gctcgccaag gggcacgact atcccaagct
     1081 cacgctggtc ggcgtgctcg attgcgacag cgcgctcttc agcgccgact tccgggcttc
     1141 cgagcggctc ttctcgcagc tggtgcaggt ctcgggacgc gcggggcggg gcgacgcgcc
     1201 gggcgaggtg ctgatccaga cggacttccc cgcgcatccg ctcttcgccg cggtggcgcg
     1261 ccaggattac gcgcgcttcg ccgaggacgc cctcgaggag cggcgcctcg cgggctttcc
     1321 gcccttctcg caccttgcgc tgttgcgcgc cgaatcgagg aaggcgggcg aggccacggt
     1381 tttcctcaag ctcgccgcgc gcctcgcgaa acgcctcgcg tcgcggcgcg aagcgaagga
     1441 catcgaggtc ttcgacccgg tccccgcgcc gctcgagcgc aaggccggat acgagcgcgc
     1501 gcagctgctg gtgcgcgcgg cctcgcgcgc gacgctgcaa cccttcctgc acgagtggag
     1561 gctgcagctc gtcgacaagg gcgggcgcca tatccgctgg tcgctcgatg tcgatcccca
     1621 ggaagtctga ggcggcgcgc acgtgccggc ccgtataatg cggggtttgg atttcccttc
     1681 ccgctagtg
//
LOCUS       DASVII010000409         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_629830,
            whole genome shotgun sequence.
ACCESSION   DASVII010000409 DASVII010000000
VERSION     DASVII010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629830"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF309_05360"
     CDS             complement(<1..822)
                     /locus_tag="VF309_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7466048.1"
                     /translation="MPNDVKIDYKRTLNLTETPFPMRGDLARREPQWIKAWQDTKLYE
                     RIRAHARGRVKYVLHDGPPYANGDIHIGHAVNKILKDLVVKSKTLAGFDAPYTPGWDC
                     HGLPIEHVIEKKHGRNLEPNKARALCRAYAAEQIERQKADFQRLGVLGDWEHPYKTMD
                     FATEGSEIRALGRIWKTGLLYRGLKPVNWCIDCASALAEAEVEYEDKTSPAVDVAFAA
                     VDAGAVAGAFDARERPERAFAVIWTTTPWTLPGNRAIAVGADVEYELVDTERGALI"
     gene            complement(842..1759)
                     /locus_tag="VF309_05365"
     CDS             complement(842..1759)
                     /locus_tag="VF309_05365"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX7466049.1"
                     /translation="MKILRHAASPAPTAIAIGSFDGVHRGHQAMLARVGEAARERGIS
                     AAALTFEPLPREYFAPLAAPARLSSLAEKLAAIAASDIGIAFVERFGARFAALTAGEF
                     ESRLARHHRARWVMVGQDFRYGAGRKGDSASLAAAGLALGFEVEVLPSVTIEGARVSS
                     TRVREALAAGDFAAASVMLARPYAICGRVVHGARRGRVMGFPTANVRLARPKAAVQGI
                     FAVKCFGAATRGLEGVASLGINPAVRIGGPATLEAFLFDFSGDLYGARLTIEFVKKLR
                     DEAHFASLDELRAQIGRDCDAARELFRAP"
     gene            complement(1766..>2187)
                     /locus_tag="VF309_05370"
     CDS             complement(1766..>2187)
                     /locus_tag="VF309_05370"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX7466050.1"
                     /translation="PGFYAKQNIRTPVKIALATLVVTQLLNAALVPYLRHAGLTLSIS
                     LGACFNAGWLWYVMHRSGSYRPEPGWAAFLVKLAVALYLMGGALWYSMGSESSWFEIA
                     TGARALKLTLVIAAGTVTYFASLALMGFRVRDFHRHE"
BASE COUNT          333 a          761 c          760 g          333 t
ORIGIN      
        1 gatgagggcg ccgcgttcgg tatcgaccaa ttcgtattcg acgtcggcgc ccaccgctat
       61 cgcccggttc ccggggagcg tccatggcgt ggtggtccag atcaccgcga atgcgcgttc
      121 gggcctttcc cgcgcatcga atgccccggc caccgcgccc gcgtccaccg cggcgaacgc
      181 cacgtcgacc gcgggcgacg tcttgtcctc gtactcgacc tcggcctcgg cgagcgcgga
      241 ggcgcagtcg atgcaccagt tcaccggctt caatccgcgg tacaggagcc ccgtcttcca
      301 gatgcggccg agcgcgcgga tctccgagcc ttcggtcgcg aagtccatcg tcttgtacgg
      361 atgctcccag tcgccgagca cgcccaggcg ctggaaatcc gccttctgcc tttcgatctg
      421 ctcggcggcg taggcgcggc agagcgcgcg ggccttgttc ggctcgagat tgcgcccgtg
      481 cttcttctcg atcacgtgct cgatcggaag tccgtggcag tcccatcccg gcgtgtacgg
      541 cgcgtcgaag cccgcgagcg tcttcgactt cacgacgagg tccttcagga tcttgttgac
      601 cgcgtggccg atgtgaatgt cgccgttggc gtagggcggc ccgtcgtgca gcacgtattt
      661 cacgcggccg cgggcgtggg cgcggatgcg ctcgtagagc ttcgtgtcct gccacgcctt
      721 gatccactgg ggctcgcgcc gggcgaggtc gccgcgcatc gggaacggcg tctccgtgag
      781 gttcagcgtg cgcttgtagt cgatcttcac gtcgttcggc atcaggatgc tctcaccagg
      841 ttcacggcgc gcggaacagc tcccgcgccg cgtcgcaatc gcgcccgatc tgcgcgcgca
      901 gctcgtcgag cgaggcgaaa tgcgcctcgt cgcgcagctt cttcacgaac tcgatggtga
      961 ggcgagcgcc gtaaaggtcg cccgagaaat cgaagaggaa tgcttcgaga gtcgccgggc
     1021 cgccgattcg cacggccggg ttgattccga ggctcgccac tccctcgagt ccgcgagttg
     1081 ctgcaccgaa gcattttacc gcaaagatgc cttgcaccgc cgccttcggg cgggcgaggc
     1141 gcacgttggc cgtcgggaag cccatcaccc gcccgcgccg cgcgccgtgc acgacgcggc
     1201 cgcagatcgc gtaggggcgc gcgagcatga cggacgcggc ggcgaaatcg ccggccgcga
     1261 gcgcttcgcg cacgcgcgtg ctcgagacgc gcgcgccctc gatcgtcacg gagggaagca
     1321 cctcgacctc gaagccgaga gcgaggcccg cggccgcgag ggacgcgcta tcgccctttc
     1381 gtcccgcgcc gtagcggaaa tcctggccca ccatcaccca gcgcgcgcga tggtggcgcg
     1441 ccagccggga ctcgaattcc ccggcggtga gcgccgcgaa gcgcgcgccg aagcgctcga
     1501 cgaacgcgat gccgatgtcg ctcgccgcga tggccgcgag cttttccgcg aggctcgaca
     1561 ggcgcgccgg cgcggcgagc ggggcgaagt actcgcgagg cagcggctcg aaggtgagcg
     1621 cggcggcgga aatcccgcgc tcgcgcgcgg cctccccgac ccgagcgagc atcgcctggt
     1681 ggccgcgatg cacgccgtcg aagctgccga tggcgatcgc ggtcggggcg ggcgaagcgg
     1741 cgtgccggag gatcttcacg cgcgctcact cgtgccgatg gaagtcccgc acacggaacc
     1801 ccatcagcgc gagggacgcg aagtacgtga cggtgccggc ggcgatgacg agggtgagct
     1861 tcagcgcgcg cgccccggtg gcgatctcga accaggacga ctcgcttccc atcgagtacc
     1921 agagggctcc tcccatcagg tagagcgcga cggcaagctt caccaggaac gcggcccacc
     1981 ccggctcggg ccggtacgac cccgagcgat gcatcacgta ccagagccag cccgcgttga
     2041 agcatgcgcc cagcgagatc gaaagcgtga gccccgcgtg gcgcaggtag ggcacgagcg
     2101 ccgcgttcag gagctgcgtc acgacgagcg tcgcgagggc gatcttcacc ggcgtgcgga
     2161 tgttctgctt cgcgtagaag cccggcg
//
LOCUS       DASVII010000410         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_19340, whole
            genome shotgun sequence.
ACCESSION   DASVII010000410 DASVII010000000
VERSION     DASVII010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19340"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VF309_05375"
     CDS             complement(<1..743)
                     /locus_tag="VF309_05375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7466051.1"
                     /translation="MSELPFTGERFVPGAKGEIWVEHWHRYHFASRWCAGKRVLDVAC
                     GEGYGTALLARQAAHVTGVDISAEAIAHARGRYASLANAEFTEASCTALPLADASVDV
                     AVSFETIEHIAAQAEFIGELARVLKPGGVLLLSCPNKLEYSDKRGFRNEFHVKELYRD
                     ELASLVASRLPEVAWYGQQASFFSVIAPEGAVSRAEGELVETGEADASQATHALSRPL
                     YFLVVASRSAASIASLPPALSVLADRDDWV"
     gene            complement(740..>2126)
                     /locus_tag="VF309_05380"
     CDS             complement(740..>2126)
                     /locus_tag="VF309_05380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7466052.1"
                     /translation="GAALLVRRKAFDEIGGFEPRIFMYGEDVDLSWRLRAKGWRVVYR
                     PQLAVVHRTYKEAHEVKPLQVLGGVSSNLALRARYGGIVRTLEGLVMLCAEILAPPSF
                     PGRRMGLAKAGLGFLWRWPYFAWTSVWPTAQFKPHFHGWSYELRRDGAFVEFASRREN
                     PGRARPLVSILIRTVDRPAWLREALTSCANQTYENLEVVVIEDGPDKSRAIVDEFRDR
                     LAIRYRATGERTGRAHAGNLALAEARGEWLNFLDDDDLFFADHVEVLVDAVQRADVAG
                     AYALAWETQTEFLDRQDAQYEEVMHVTRHYQRFDRLTLWHHNYLPIQAVLFSRTLFEQ
                     HGGFDEEMDQLEDWNLWTRYTLEDDFVLVEKTTSKYRVPANSRIAAERQALLDLAYRD
                     ALERQRSLRVTLSPRAISEMADAYVRGQAVMMVTRNDLRRFMSSNRMLARLAAYRQPV
                     RRALRRLGLLR"
BASE COUNT          333 a          736 c          758 g          299 t
ORIGIN      
        1 acccagtcgt cgcgatcggc gagcaccgag agcgcgggcg ggagcgaggc gatcgacgcc
       61 gcgctgcggc tcgcgacgac gaggaagtag agcgggcgcg acagcgcgtg ggtcgcctgg
      121 ctcgcatcgg cctcgccggt ctcgacgagc tcgccttcgg cccgcgagac ggccccttcg
      181 ggagcgatca ccgagaagaa cgacgcctgc tgcccgtacc acgccacttc gggcaggcgc
      241 gaggcgacca ggctcgcgag ctcgtcgcgg tagagctcct tcacgtggaa ctcgttcctg
      301 aagccgcgct tgtcgctgta ttcgagcttg ttcgggcacg acagcagcag cacgccgccg
      361 ggcttcagca cgcgcgccag ctcgccgatg aattcggcct gcgcggcgat gtgctcgatc
      421 gtctcgaacg agaccgcgac gtcgacgctc gcgtcggcga gcggcagcgc ggtgcacgaa
      481 gcctccgtga actcggcgtt ggcgagcgac gcatagcggc cgcgggcatg ggcgatcgcc
      541 tcggccgaga tgtcgacgcc ggtcacgtgc gccgcctggc gcgcgagaag cgccgttccg
      601 tagccttcgc cgcacgccac gtcgagcacg cgcttgcccg cgcaccaccg cgacgcgaaa
      661 tggtagcgat gccagtgctc gacccagatc tcgcccttgg cgcccgggac gaaacgctcg
      721 ccggtgaagg gaagctcgct caccgcagga gccccaagcg gcgcagcgcg cgccgcacgg
      781 gctggcgata cgccgccagc cgcgcgagca tgcggttcga cgacatgaag cggcgcaggt
      841 cgttgcgggt caccatcatc accgcctgcc cgcgcacgta ggcgtcggcc atctcggaga
      901 tggcgcgcgg cgagagggtg acgcgcagcg agcgctgccg ctcgagcgcg tcgcggtagg
      961 cgaggtcgag gagcgcctgg cgctccgcgg cgatgcgcga attcgcgggg acgcggtact
     1021 tggacgtggt cttctccacc agcacgaagt catcctccag cgtgtagcgc gtccacaggt
     1081 tccagtcctc gagctggtcc atctcctcgt cgaagccgcc atgctgctcg aagagggtgc
     1141 gactgaacag cacggcctgg atggggaggt aattgtggtg ccacagcgtg agccggtcaa
     1201 agcgctggta atggcgcgtg acgtgcatga cctcctcgta ctgcgcgtcc tgccggtcga
     1261 ggaactcggt ctgcgtctcc caggcgagcg cgtacgcccc cgcgacgtcc gcgcgctgca
     1321 cggcgtccac gagcacctcg acgtgatcgg cgaagaagag atcgtcgtcg tcgaggaagt
     1381 tcagccactc gccgcgcgcc tccgcgaggg cgaggttgcc cgcgtgcgcg cgtcccgtgc
     1441 gctcgccggt ggcgcgataa cggatcgcga ggcggtcgcg gaattcgtcc acgatggcgc
     1501 gcgacttgtc ggggccgtcc tcgatgacca ccacctcgag gttctcgtag gtctggttcg
     1561 cgcagctcgt gagcgcctcg cgcagccacg ccggccggtc cacggtgcgg atgaggatcg
     1621 acaccagggg acgcgcgcgg ccggggttct cgcggcgcga cgcgaactcg acgaaggcgc
     1681 cgtcgcggcg cagctcgtag ctccagccgt ggaaatgcgg cttgaattgc gcggtcggcc
     1741 agacgctggt ccatgcgaag tagggccatc gccagaggaa gccgaggccc gccttcgcga
     1801 gccccatgcg ccgccccgga aacgagggcg gcgcgaggat ctcggcgcac agcatgacga
     1861 ggccttcgag cgtgcgcacg atgccgccgt agcgcgcgcg cagcgcgagg ttgctcgaca
     1921 cgccgccgag cacctgcagc ggcttcacct catgggcctc cttgtaggtg cggtgcacca
     1981 ccgcgagctg cgggcggtag accacgcgcc agccctttgc ccgcaggcgc cacgaaagat
     2041 ccacgtcctc gccgtacatg aagatgcgcg gctcgaagcc gccgatctcg tcgaacgcct
     2101 tgcggcgcac gaggagggcc gcgccg
//
LOCUS       DASVII010000411         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_126895,
            whole genome shotgun sequence.
ACCESSION   DASVII010000411 DASVII010000000
VERSION     DASVII010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126895"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF309_05385"
     CDS             complement(<1..317)
                     /locus_tag="VF309_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466053.1"
                     /translation="MKTNSLQFKVLVLMAGAMAFALAISVFALTRVYGSIQELDRISR
                     EDFETQQAIDRAAVAFKQQVQEWKDVLLRGDDPQKLDKYWSAFLEQEKAVDDMVREAR
                     SATP"
     gene            complement(331..1665)
                     /locus_tag="VF309_05390"
     CDS             complement(331..1665)
                     /locus_tag="VF309_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7466054.1"
                     /translation="MSHKPSYADAGAANDMPARIGNYEIQGILGRGQSATIYLGRELF
                     PARDVAIKVYDAHHLDPEDRKVFRSLFLKETLLARKLTHPNITQVYDAAADDDRAYIV
                     MEYAQEGSLDRYCVPEGLLKPERVAHLLEHVCDALSYAHANGIIHRDLKPANVLMSAD
                     GEAKVADFGVAFSNFAFDATRGMNVGSPAYMAPEQFERKPASMQTDVYAVGIMMYKML
                     TGALPFPLDTPAALTTRILLGNFPPPSAARPGLPPVFDAIFRRSTARDASNRYRTWEE
                     FASDLRTVSQPGADMADADQRVATMKSLPFFHDFDAASLAEAAPMARWFDVRAGSQLV
                     GEDDPGYSFFVLVRGQMRITRRGTLLAIRGAGECLAEIAFLRRSGARRFSSIAAVTDC
                     TVMEFDPDVLWLASPECTRNFHQAFLHTMADRLVAAEGALAEMLGGKNVTLF"
     gene            complement(1662..2141)
                     /locus_tag="VF309_05395"
     CDS             complement(1662..2141)
                     /locus_tag="VF309_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7466055.1"
                     /translation="MSSQADLERREFLTCHLGAESYGVDILLVQEIREVAEVTRVPHV
                     SRFVRGVVNLRGAIVPIVDLGLMFGLPEPLDLKTASAIVLNVDQRMVGLIVSSVSDVV
                     ALAPDEILPAPELAAREVSAAIAGIGSRRGAQAADSESSILLLDVEHIMRRVREERE"
     gene            complement(2138..>2927)
                     /gene="cheB"
                     /locus_tag="VF309_05400"
     CDS             complement(2138..>2927)
                     /gene="cheB"
                     /locus_tag="VF309_05400"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEX7466056.1"
                     /translation="LELGAVDFVTKPKLNEQAPDDYGQMIADKIRAAKSARLKAPRRP
                     DEPSAAEMTMPPVMKRPVPVGVKTSERIIVVGASTGGTEAIREFLVGMPPDCPGILIV
                     QHMPENFTRMFAERLNGLCKINVKEAEHNDPILPGHAYVAPGGKHLWVKREGGQMLAK
                     LSTEPPMNLHRPSVDFLFMSCAKFVGADAIGVIMTGMGRDGAKGMLEMKAAGSWNIAQ
                     DEASSVIFGMPREAIEAGAVHEVAPLARLRERALARFSKKDAAR"
BASE COUNT          441 a          987 c         1016 g          483 t
ORIGIN      
        1 ggcgtggccg agcgcgcttc gcgcaccatg tcgtccaccg ccttttcctg ctcaaggaat
       61 gcgctccagt acttgtcgag cttctgggga tcatcgccgc gaagcaggac atccttccac
      121 tcctgcacct gctgcttgaa cgcgacggcg gcgcggtcga tcgcctgctg cgtctcgaaa
      181 tcctcgcggc tgatgcggtc gagctcctgg atggatccgt acacgcgcgt gagcgcgaag
      241 accgagatcg cgagggcgaa ggccatcgcc ccggccatga gcacgagcac cttgaattga
      301 aggctgttgg ttttcatgtc gtcggcgaaa tcagaatagg gtcacgttct ttccgccgag
      361 catctcggcg agcgcgccct cggcggcgac gaggcggtcc gccatcgtgt gcaggaacgc
      421 ctggtggaaa ttgcgggtgc actcgggcga ggcgagccag agcacgtcgg gatcgaattc
      481 catcacggtg caatcggtga ccgcggcgat ggagctgaag cggcgcgcgc ccgagcggcg
      541 caggaacgcg atctccgcga ggcactctcc cgcgccgcgg atcgcaagga gcgttccgcg
      601 ccgcgtgatg cgcatctggc cgcgcacgag cacgaagaag gaatagccgg ggtcgtcctc
      661 gcccaccagc tgcgagcccg cgcgcacgtc gaaccagcgg gccatcggcg ccgcctcggc
      721 gaggctcgcg gcgtcgaagt cgtggaagaa cggcagcgat ttcatggtcg ccacgcgctg
      781 gtcggcatcg gccatgtccg cgccgggctg cgagacggtg cgcaggtccg aggcgaattc
      841 ctcccacgtg cgatagcggt tcgacgcatc gcgcgccgtg gagcggcgga agatcgcgtc
      901 gaagaccggc ggcaggcccg ggcgcgccgc cgagggcggc gggaagttcc cgagaaggat
      961 gcgcgtcgtg agggccgcgg gcgtgtcgag gggaaacggc agcgcgccgg tgagcatctt
     1021 gtacatcatg atgcccaccg cgtagacgtc ggtctgcatc gacgcgggct tcctctcgaa
     1081 ctgctcgggc gccatgtacg ccggcgagcc cacgttcatg ccgcgcgtcg cgtcgaacgc
     1141 gaagttcgag aaggcgacgc cgaagtccgc gaccttcgcc tcgccgtcgg cgctcatgag
     1201 cacgttggcc ggcttcaggt cgcggtggat gatgccgttg gcgtgggcgt agctcagggc
     1261 gtcgcacacg tgctcgagca ggtgcgcgac gcgctccggc ttgagcagcc cttcggggac
     1321 gcaatagcga tcgaggctgc cttcctgcgc gtattccatc acgatgtagg cgcgatcgtc
     1381 gtcggcggcc gcgtcgtaga cctgcgtgat gttggggtgc gtgagcttcc tcgcgaggag
     1441 cgtctccttg aggaagagcg agcggaacac cttgcgatcc tcgggatcca ggtgatgcgc
     1501 gtcgtagacc ttgatggcga cgtcgcgcgc ggggaagagc tcgcgtccca ggtagatggt
     1561 cgccgactgg ccccgcccga ggatgccctg gatctcgtag ttgccgatgc gcgccggcat
     1621 gtcgttcgcc gcccccgcgt cggcgtagct gggcttgtgg ctcattcgcg ctcctcccgc
     1681 actcgccgca tgatgtgctc gacgtcgagg agcaggatgc tcgattcgga gtcggctgcc
     1741 tgcgcgccgc ggcgcgagcc gatgccggcg atcgccgcgc tcacctcgcg cgccgcgagc
     1801 tcgggcgcgg gcaggatctc gtcgggcgcg agcgcgacca catcggatac gctcgagacg
     1861 atgagcccca ccatgcgctg gtcgacgttc agcacgatcg cgctcgcggt cttcaggtcg
     1921 agcggttccg gaaggccgaa catgaggccc aggtcgacga tcggcacgat cgcgccgcga
     1981 aggttcacga cgccgcgcac gaagcgcgag acgtggggca cgcgcgtgac ctcggccacc
     2041 tcgcgaatct cctgcacgag gaggatgtcg acgccgtagc tctcggcgcc gagatggcac
     2101 gtgaggaact cgcggcgctc gagatcggcc tgggagctca tcgcgcggcg tcctttttcg
     2161 agaatcgcgc gagcgcgcgc tcgcgcaggc gcgccagcgg cgcgacttcg tgcaccgcgc
     2221 ccgcctcgat ggcctcgcgc ggcatgccga agatcacgct cgaggcctcg tcctgcgcga
     2281 tgttccacga gcccgcggcc ttcatctcga gcatgccctt cgcgccgtcg cggcccatgc
     2341 cggtcatgat gacgccgatc gcgtcggcgc cgacgaactt cgcgcacgac atgaagagga
     2401 agtccaccga ggggcggtgc aggttcatcg gcggctcggt cgagagcttg gcgagcatct
     2461 ggcccccctc gcgcttcacc cacaggtgct tgccgcccgg cgccacgtag gcgtgacccg
     2521 ggaggatcgg atcgttgtgc tcggcttcct tcacgttgat cttgcacagg ccgttcagcc
     2581 gctcggcgaa catgcgcgtg aagttctccg gcatgtgctg cacgatgagg atgccggggc
     2641 aatccggcgg catgccgacc aggaactcgc gaatcgcttc cgtcccgccg gtcgacgcgc
     2701 cgaccacgat gatccgctcg ctcgtcttca cgcccaccgg caccggccgc ttcatcaccg
     2761 gcggcatggt catttcggcc gccgagggct cgtccggccg ccgcggcgcc ttcagccgcg
     2821 cgctcttcgc cgcgcggatc ttgtcggcga tcatctggcc gtagtcgtcg ggcgcctgct
     2881 cgttcagctt cggcttcgtc acgaaatcca ccgcgcccag ctcaagc
//
LOCUS       DASVII010000412         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_522183,
            whole genome shotgun sequence.
ACCESSION   DASVII010000412 DASVII010000000
VERSION     DASVII010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522183"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF309_05405"
     CDS             complement(<1..523)
                     /locus_tag="VF309_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7466057.1"
                     /translation="MSVAPLHFIDAGAGPAVVLVHAIGCDLRMWDELAADLVANGMRV
                     VRVDVRGHGASPVPERPYSLAELAGDVAAVLDRCGVARAHWVGLSMGGMIGQAFAIER
                     GERLARLVLANTTSSYGADGRKMWEARAKAVEDGGMAAITDLAMTRYFSDEFRARRAD
                     VVERVRARFLETNP"
     gene            complement(520..927)
                     /gene="pcaC"
                     /locus_tag="VF309_05410"
     CDS             complement(520..927)
                     /gene="pcaC"
                     /locus_tag="VF309_05410"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR02425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX7466058.1"
                     /translation="MSRDAYVKGMKTRRKVLGDEWVDRAERNKDAFNAEFQELITRYA
                     WDEIWNRKGLPHGTRRMLVIATMVALGRWEEFQLHVRAALQSGDLSRDDIKEVLLQSA
                     VYCGVPAANTAFKEARAVIGEVSKVKNGKKRGK"
     gene            complement(948..>1988)
                     /locus_tag="VF309_05415"
     CDS             complement(948..>1988)
                     /locus_tag="VF309_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX7466059.1"
                     /translation="VISAASAAAIAAACERIDVDVEGLVREAKRSATLAVPLVKALVA
                     EVSLVSKPAAVHVHFAATSQDALDTAMALCLKSCLEEADRELDAAVRALARRAREHRA
                     TPMLGRTLMQPALSITAGLKIARWAVALAHDRERIAEAQSRGLAVQFGGPVGALDALG
                     AKGPAVRHRMALQLGLADAPAWHVHRNAWVDLLDRVGQAVLTAGKIARDVSLLAQPEV
                     GEMLEALPQPGVGASSSMPHKRNPVACVHALAAATRMPGLLATLHAAGIAEHERALGG
                     WQAELALVPEIAAALGTSLDFLGTIATSLVVNPAPMKANLSEYGEAGGAKAEAVQPAV
                     DELLAALTPYLS"
BASE COUNT          281 a          722 c          686 g          299 t
ORIGIN      
        1 gcgggttcgt ctcgaggaag cgggcgcgga cgcgctcgac cacatcggcg cggcgcgccc
       61 ggaattcgtc ggagaaatag cgcgtcatcg cgagatcggt gatcgccgcc atgcctccgt
      121 cctcgaccgc cttggcgcgc gcctcccaca tcttgcgccc gtcggcgccg taggagctcg
      181 tcgtattcgc gagcacgagc ctggcgaggc gctcgccgcg ctcgatcgcg aacgcctggc
      241 cgatcatccc gcccatcgac aatcccaccc aatgggccct ggcgacgccg catcgatcga
      301 gcacggccgc gacgtctccg gcgagctcgg cgagggaata ggggcgctcg gggaccgggc
      361 tcgcgccatg gccgcgcacg tcgacgcgca cgacgcgcat gccgttcgcg acgaggtccg
      421 ccgcgagctc gtcccacatg cgcaggtcgc agccgatggc gtgcaccagc acgaccgcgg
      481 ggcccgcgcc cgcgtcgatg aaatgaagcg gcgcgacgct cacttcccgc gcttcttccc
      541 gttcttcacc ttcgagacct ccccgatcac cgcgcgcgct tccttgaacg cggtattggc
      601 cgccggcacg ccgcaataga ctgccgattg gaggagcact tccttgatgt cgtcccggga
      661 gaggtctccc gattgcagcg ccgcgcgcac atgcagctgg aactcttccc agcggccgag
      721 cgccaccatc gtcgcgatca ccagcatgcg gcgcgtcccg tgcggcaggc ccttgcggtt
      781 ccagatctcg tcccacgcgt aacgggtgat gagttcctgg aactcggcgt tgaaggcgtc
      841 cttgttgcgc tcggcccggt ccacccattc gtctcccagg accttgcggc gcgtcttcat
      901 tcccttcacg tatgcatctc ggctcatcgc gacgcgcctt ctcgaggtca ggaaagatag
      961 ggggtgaggg cggcgaggag ctcgtcgacg gcgggttgca ccgcctcggc cttggcgccg
     1021 ccggcctcgc cgtattccga gaggttcgcc ttcatgggcg cggggttcac cacgagcgag
     1081 gttgcgatgg tgccgaggaa gtcgaggctc gttccgagcg ccgcggcgat ctcgggcacc
     1141 agcgcgagct cggcctgcca tccgcccagg gcgcgctcat gctcggcgat tcccgcggcg
     1201 tgcagcgtcg cgaggagtcc cggcatccgc gtcgcggcgg cgagcgcgtg cacgcaggca
     1261 acgggattgc gcttgtgggg catcgagctc gaggcgccca cgccgggctg tggaagcgcc
     1321 tcgagcattt cgcccacttc gggctgcgcg agaagcgaca cgtcgcgcgc gatcttgccc
     1381 gcggtgagga ccgcctgccc cacgcgatcg aggagatcga cccaggcgtt gcggtgaacg
     1441 tgccacgcgg gcgcatcggc gagccccagc tgcagcgcca tgcgatggcg caccgccggg
     1501 cccttggcgc cgagcgcgtc gagcgcgccg acgggaccgc cgaactgcac cgccagtccg
     1561 cgcgactgcg cctccgcgat gcgctcgcgg tcgtgcgcga gggccacggc ccatcgcgcg
     1621 atcttcagtc ccgcggtgat cgacagcgcg ggctgcatca acgtgcgccc caacatggga
     1681 gtcgcgcgat gctcgcgggc gcggcgggcg agggcgcgca ccgccgcatc gagctcccgg
     1741 tcggcttcct cgagacagct cttcaggcaa agcgccatcg cggtgtcgag cgcatcctgg
     1801 ctggtggccg cgaagtgcac gtgcaccgcg gctggcttcg acacgagcga gacttcggcg
     1861 acgagggcct tcacgagcgg caccgcgagc gtcgcggagc gcttcgcctc gcgcaccagg
     1921 ccctcgacgt cgacgtcgat gcgctcgcat gcggcggcga tcgcggccgc gctcgcggcc
     1981 gaaatcac
//
LOCUS       DASVII010000413         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_162500,
            whole genome shotgun sequence.
ACCESSION   DASVII010000413 DASVII010000000
VERSION     DASVII010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162500"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VF309_05420"
     CDS             <1..945
                     /locus_tag="VF309_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466060.1"
                     /translation="MACPEVFQISSFAFLRLADEERDLLPRAVARAVPSMQALTPGYL
                     GTLEGATQVRASELPWWAYSPLDAAARRMPGKVFLLLVMVSILAAPIAFLAAIAWARP
                     WRGEPRAGLLLALLLGGTVAHGVVTTVFGGGLNEAARHFIPGALAMYVLLIAAIVGIP
                     GAVARWMAEPKARAFEMAAAAALVPVVVLSCAAAFRWAQVQPLAIGALELPAGREATL
                     PALKLHGWALDPVGVESVQVEIGKLKRQVQPEEDSPDLRAAYPGYPDRAKARFTVELG
                     ADALAAAGAPEELPLRITVRGVDGAVTEIDRRRLDFPK"
     gene            1074..1532
                     /locus_tag="VF309_05425"
     CDS             1074..1532
                     /locus_tag="VF309_05425"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7466061.1"
                     /translation="MKSFAAVLMVFLATAFHAVADDVSRGTPDEAKAMVKKAVAHFKK
                     VGKDKALPDLCKTDGPFVDRDLYVTAIAMDGLEVAHINPKTVGKNVMDLRDPDGKYFI
                     KERLDAAKAASSGWQEFKFFNPVTRKVEAKTAYWERVGDLVFSSGAYKPI"
     gene            complement(1557..>2836)
                     /locus_tag="VF309_05430"
     CDS             complement(1557..>2836)
                     /locus_tag="VF309_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466062.1"
                     /translation="FSIIRLQGKTSNFRVYGNGVGTPRKIDQYTVELTTPVPNPVMLE
                     MLANSLFMMSKAWCEKNNAVNTQDFTNEKEAYTARNAMGTGPFILVSRDPDVKSVFKK
                     NPNWWGLKENRFDGNVDEIVYTPIGSAGTRMAALLAGNIDFVLDPPPSDVDKLKQEKD
                     IKIYEGRENRIVFLQMDQARDELLYSNVKGKNPFKDVRVRKAFYQAIDVYAIQKAVMR
                     GLSVPTAINLPNPDKAGIPKSMDKRYPYDIAAAKKLLAEAGYPNGFETQLDCPNNRYI
                     NDEKICVAVAGMLAKIGVTIKVNAIPRAQYFPKAQRMEISFCMLGWGGATTDAIFTLQ
                     PVLHSRNDKGDGDYNWGNYKDDAFDAMIDDAKGDTDPKHRQETINKAMQYFHDHVFMI
                     PLHLQVIPWASRSNVEVVHRADNWLQTTWVKIK"
BASE COUNT          416 a          923 c          958 g          539 t
ORIGIN      
        1 atggcgtgcc ctgaggtctt ccagatttcg agcttcgcct tcctgcgcct cgcggacgag
       61 gagcgcgatc tgctgccgcg cgccgtggcg cgagccgtcc cctcgatgca ggcgctgacg
      121 ccgggctacc tcggaacgct cgaaggcgcg acgcaggtca gggcgagcga actgccgtgg
      181 tgggcgtatt ccccgctcga cgccgcggcg aggcgcatgc cgggcaaggt gttcttgctg
      241 ctcgtgatgg tgtcgatcct cgccgcgccg atcgccttcc tcgccgcgat cgcgtgggcg
      301 cggccgtggc ggggagagcc gcgcgcgggc ctgctgctcg cccttctcct cggcggaacc
      361 gtggcgcacg gtgtcgtcac caccgtgttc ggcggcgggc tgaacgaggc ggcgcgccat
      421 ttcattcccg gcgcgctcgc catgtacgtg ctcctcatcg cggccatcgt gggcattccg
      481 ggagcggtgg cgcgttggat ggcggagccg aaggcgcgcg cgttcgagat ggccgccgcg
      541 gccgccctcg tgccggtcgt ggtgctctcg tgcgcggcgg cgtttcgctg ggcccaggtg
      601 cagccgctcg caattggcgc gctcgagctt cccgcgggcc gcgaggcgac gctccctgcg
      661 ctgaagctgc acggctgggc gctcgatccc gtgggcgtcg aatcggtgca ggtcgagatc
      721 ggaaagctga agcgccaggt gcagcccgag gaagacagcc ccgacctgag ggccgcctac
      781 ccgggatacc ccgaccgcgc gaaagcccgc ttcacggtcg agctcggcgc cgacgctctt
      841 gccgccgccg gcgcgcccga ggagctgccg ctacgcatca ccgtgcgcgg cgtcgacggc
      901 gccgtgaccg agatcgaccg ccggcggctg gatttcccga agtagtcggg cccctgcgcg
      961 gatattccgc acgcgccgat tacgcctgaa atcgtccgga gtgacgccgt tcacacgcgg
     1021 cgcgccgccg catcgctatc ttctcggaag tgattcattc cccgaggtcg tcgatgaaaa
     1081 gcttcgccgc cgtgctgatg gtatttctcg caaccgcctt ccatgccgtc gccgacgacg
     1141 tctcgcgcgg cacgcccgac gaggcgaagg cgatggtgaa gaaggccgtc gcccatttca
     1201 agaaggtcgg caaggacaag gccctccccg acctctgcaa gaccgacggg cctttcgtgg
     1261 accgcgacct ctacgtgacg gcgatcgcca tggacggcct cgaggtcgcc cacatcaatc
     1321 cgaagacggt cggcaagaac gtgatggacc tgcgcgaccc cgacggcaag tacttcatca
     1381 aggagcggct cgacgccgcg aaggccgcct cgagcggctg gcaggagttc aagttcttca
     1441 accccgtgac gcgcaaggtc gaggcgaaga ccgcctactg ggaacgcgtg ggcgacctcg
     1501 tgttctcgag cggggcgtac aagcccatct agggcccgta cgccgcggcc cggcgattac
     1561 ttgatcttga cccaggtggt ctgaagccag ttgtccgcgc ggtgcacgac ctcgacgttc
     1621 gagcgcgagg cccacggaat cacctgcagg tgcagcggga tcatgaagac gtggtcgtgg
     1681 aagtactgca tcgccttgtt gatcgtctcc tggcgatgct tgggatcggt gtcgcccttg
     1741 gcgtcgtcga tcatcgcgtc gaacgcgtca tccttgtagt tgccccagtt gtagtcgccg
     1801 tcgcccttgt cgttgcgtga gtgcagcacc ggctgcagcg tgaagatggc gtcggtggtg
     1861 gcgcctcccc agcccagcat gcagaagctg atctccatgc gctgcgcctt cgggaagtac
     1921 tgggcgcgcg ggatagcgtt caccttgatc gtcacgccga tcttcgcgag catccccgcc
     1981 accgcgacgc agatcttctc gtcgttgatg tagcggttgt tggggcagtc gagctgcgtc
     2041 tcgaagccgt tcggatatcc cgcctcggcg agcagcttct tggcggcggc gatgtcgtac
     2101 gggtagcgct tgtccatcga cttcgggatc ccggccttat cgggattcgg caggttgatc
     2161 gccgtcggca ccgagaggcc gcgcatcacc gccttctgga tcgcgtacac gtcgatcgcc
     2221 tggtagaagg ccttgcgcac gcgcacgtcc ttgaaggggt tcttgccctt cacgttcgag
     2281 tagagcagct cgtcgcgcgc ctggtccatc tgcaggaaga cgatgcggtt ctcgcggccc
     2341 tcgtagatct tgatgtcctt ttcctgcttg agcttgtcga cgtcgctggg cgggggatcg
     2401 aggacgaagt cgatgttgcc ggccagcagc gccgccatgc gcgtgcccgc ggagccgatg
     2461 ggcgtgtaga cgatctcgtc gacgttaccg tcgaagcggt tctccttcag gccccaccag
     2521 ttcgggttct tcttgaagac gctcttcacg tcgggatcgc gtgagacgag gatgaaaggc
     2581 cccgtgccca tcgcgttcct cgccgtgtac gcttccttct cgttggtgaa gtcctgcgtg
     2641 ttgacggcgt tgttcttctc gcaccacgcc ttgctcatca tgaagaggct gttggcgagc
     2701 atctcgagca tcaccgggtt ggggaccggc gtggtgagct cgaccgtgta ctggtcgatc
     2761 ttccgcggcg tgccgacgcc gttgccgtag acgcgaaagt tggaggtctt gccctgcagc
     2821 cggatgatgg agaaga
//
LOCUS       DASVII010000414         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_594232,
            whole genome shotgun sequence.
ACCESSION   DASVII010000414 DASVII010000000
VERSION     DASVII010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594232"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF309_05435"
     CDS             complement(<1..416)
                     /locus_tag="VF309_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7466063.1"
                     /translation="MRRIATDILVLGAGGAGLFAALHAKRRAPALSVTIAVKGLLGKC
                     GCTRMVQGGYNVALAPGDSVERHFMDTIEGGKWLSDQDLAWTLVTKAVERIHELENEL
                     GCFFDRNPDGTVHQKAFAGQTFDRTVHKGDLTGIEI"
     gene            complement(413..2371)
                     /locus_tag="VF309_05440"
     CDS             complement(413..2371)
                     /locus_tag="VF309_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX7466064.1"
                     /translation="MPELATGGELSEAQLKKVEEFIEEEEGRFNRYGGWLATLLTAVA
                     VASSAFHLYAAYSIVRTDWLREIHVGIVLFLSYMLFPIAKRYRHRLMWWDVACALLAV
                     VVIVYMVWSGDDFMDRTTNPTRLDMFFGIALMALCIEAVRRTTGWVMPIIIGGFLLYA
                     FVGPWLPAPWTHRGYGISRLVGHMYMTLEGIFGTAVDVSATLIILFTIYGAFLDYSGA
                     GRFFLDFSFALLGGKPTSAGRAVVLASFLLGGPSGSGVATTVTIGTVAYPMLKKSGYA
                     KDAAGGLLAAGGLGAILSPPVLGAAAFIIAEYLNISYLEVIKMATIPTILYYFSLFLM
                     VELDARKYGMSEAAFAVAESAWTLAKRYWYHFLSLVSIVIFMLWGFSPTVSVFWATVT
                     AFAVSFFRRETAMVPSRVVKAMAAGTNGVLSVAATCAAAGIIVGVVTLTGLGLKFSSI
                     VLAYAGGSLALTALYVALIVWIVGLAVPVTASYIICAVIAAPAMIQLGVPDFAAHMFI
                     FYYALLSEVSPPTALSPFAAAAITGGDPYKTTMQAWKYTMPAFVVPFFFVLDPLGAGV
                     LLKLPAGATWLQVSWVVFMCFVAIAALAAGLQGWALRKTTWLERSMLVAGGLLVIAPS
                     DTFDVVGLAIAIGAIVLQVIRGRIAAPA"
BASE COUNT          489 a          867 c          864 g          355 t
ORIGIN      
        1 atgatctcga tgccggtcaa gtcgcccttg tgtaccgtgc ggtcgaaggt ctgcccggcg
       61 aaggctttct gatgcaccgt gccgtcggga ttgcggtcga agaagcagcc gagctcgttc
      121 tccagctcgt ggatgcgctc cacggccttg gtgacgagcg tccacgcgag gtcctggtcg
      181 gaaagccact tgccgccctc gatcgtgtcc atgaagtggc gctccaccga atccccgggc
      241 gcgagcgcga cgttgtatcc gccctggacc atgcgcgtgc atccgcactt tccgaggagg
      301 cccttcacgg cgatggtcac cgagagcgcg ggcgcgcggc gcttcgcgtg cagcgccgcg
      361 aaaagccccg cccctcccgc gccgagaacg aggatgtcgg tggcgatgcg cctcacgcgg
      421 gcgccgcgat ccggccgcga atcacctgca gcacgatcgc gccgatggcg atggcgagcc
      481 ccacgacgtc gaacgtgtcg ctgggcgcga tcacgagcag cccgcccgcg accagcatcg
      541 agcgctcgag ccaggtcgtc ttgcgaagcg cccacccctg cagccccgcg gcgagcgccg
      601 cgatcgcgac gaagcacatg aagacgaccc acgagacctg cagccacgtc gcgcccgcgg
      661 gaagcttgag gagcacgccc gcgccgagcg gatcgagcac gaagaagaac ggcaccacga
      721 aggcgggcat cgtgtacttc cacgcctgca tcgtggtctt gtacggatcg ccgccggtga
      781 tcgcggccgc ggcgaacggc gaaagcgccg tcggcggcga gacctccgag aggagcgcgt
      841 agtagaagat gaacatgtgc gcggcgaaat ccggcacgcc gagctggatc atcgcgggcg
      901 ccgcgatcac cgcgcagatg atgtacgagg ccgtgaccgg gaccgccaat cccacgatcc
      961 atacgatgag cgccacgtag agcgcggtga gcgcgaggct tcctcccgcg taggcgagga
     1021 cgatcgagga gaacttcagg ccgaggcccg tcagcgtgac gacccccacg atgatccccg
     1081 cggccgcgca cgtcgcggcg acgctcagca cgccgttggt gcccgcggcc atcgccttca
     1141 cgacgcgcga cgggaccatc gccgtctcgc ggcggaagaa gctcaccgcg aacgcggtca
     1201 ccgtcgccca gaagacgctc accgtgggcg agaagcccca cagcatgaag atcacgatcg
     1261 agacgagcga caggaagtgg taccagtagc gcttggcgag cgtccacgcg ctttccgcca
     1321 ccgcgaacgc cgcctcgctc atcccgtact tgcgcgcgtc gagctcgacc atgaggaaga
     1381 gcgagaagta atacaggatg gtcgggatcg tcgccatctt gatcacttcg agatacgaga
     1441 tgttcaggta ctcggcgatg atgaaggcgg ccgcgcccag caccggcggc gacaggatcg
     1501 ctcccaggcc tcccgcggcg agaagccctc cggccgcatc cttcgcatag cccgacttct
     1561 tgagcatcgg atacgcgacc gtgccgatgg tgacggtcgt cgcgactccc gagcccgagg
     1621 gcccgccgag gaggaacgag gcgagcacca ccgcgcggcc ggcgctcgtg ggcttgccgc
     1681 cgaggagcgc gaaggaaaaa tcgaggaaga agcgccccgc ccccgaatag tcgaggaacg
     1741 cgccgtagat ggtgaagagg atgatgagcg tcgccgaaac gtcgacggcg gtgccgaaga
     1801 tcccctcgag cgtcatgtac atgtggccca cgaggcggga gatgccgtag ccgcggtgcg
     1861 tccacggcgc cggcagccac gggccgacga aggcgtagag caggaacccg ccgatgatga
     1921 tcggcatcac ccatccggtg gtgcgccgca cggcctcgat gcagagcgcc atcaacgcga
     1981 tcccgaagaa catgtcgagg cgcgtgggat tggtggtgcg gtccatgaag tcgtcgccgc
     2041 tccacaccat gtagacgatg acgacgaccg ccaggagggc gcaggccacg tcccaccaca
     2101 tgaggcgatg gcggtagcgc ttcgcgatgg ggaagagcat gtacgagagg aagagcacga
     2161 tcccgacatg gatctcgcga agccagtcgg tgcgcacgat cgagtaggcg gcgtagaggt
     2221 ggaacgcgga gctcgcgacc gcgaccgccg tgaggagcgt ggcgagccag ccgccgtagc
     2281 ggttgaaccg gccctcctcc tcctcgatga attcctcgac cttcttgagc tgcgcttccg
     2341 acagctcccc gcccgtcgcc agctcaggca tcgcctcacc cctccgtgcg ccctcgtctc
     2401 ggggctcgag aattatgggg tcagatcccg attcaagcga gatacatcaa gagatacgca
     2461 gggcggccca cgaaatcggg ctctgacccc ggcttgcgtg cgcctaagcc cgagcgttca
     2521 ggatccacaa ccgcgcgagg tccgcggtgc cgtcggtgcc ctgcacggtc ttcag
//
LOCUS       DASVII010000415         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_522282,
            whole genome shotgun sequence.
ACCESSION   DASVII010000415 DASVII010000000
VERSION     DASVII010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522282"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1862
                     /locus_tag="VF309_05445"
     CDS             201..1862
                     /locus_tag="VF309_05445"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7466065.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRKIKVSLVKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFSSTRMTKANNFTTGQIYERVINKE
                     RRGDYLGGTVQVIPHITDEIKLSIKAACKDAEVGIIEIGGTVGDIESLPFLEAIRQMG
                     IEEGRTNTCYIHLTLVPYLDAVGEIKTKPTQHSVKELRGIGIQPDIVMCRSEVPIPES
                     DRRKIALFTNVDVRSVISAYDMDSIYKIPVALHKQGLDEIVCRKLDLAAYPADLTMWE
                     NIVDALENPEHQVDIAMVGKYIDLTDSYKSLTEALIHAGIHTRTKVKIHYIDSEQIEA
                     KGIETLKDMDAVLVPGGFGKRGIEGKIAAVKYARENKVPYLGICLGMQIAAIEIARDI
                     CGLEGANSTEFDPDTPHPVIALITEWQNHDGKVERRDEKSYLGGTMRLGAQPAAVKKG
                     TLAHRIYGKDTVAERHRHRYEVNNHYVGRLEAAGLNVSAKTKAEDLTEIAELADHPWF
                     VGVQYHPEFTSTPREGHPLFKSFINAALEQHKLRVPEPALKVVTK"
     gene            1859..>2621
                     /gene="kdsA"
                     /locus_tag="VF309_05450"
     CDS             1859..>2621
                     /gene="kdsA"
                     /locus_tag="VF309_05450"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX7466066.1"
                     /translation="MKLCGFEAGLDKPFFLIAGPCVVESEELQMDVAGKLKEMTAELG
                     IPFIFKSSYDKANRSSGKSFRGPGMEKGLEILAKVRKTLGVPILTDVHTEEEVPTVAA
                     IVDVLQTPAFLCRQTDFIHAVARAGKPVNIKKGQFLAPSDMKNVVDKAREASGADNIM
                     VCERGASFGYNNLVSDMRSLAIMRDTGCPVVFDATHSVQLPGGQGTSSGGQREFVPVL
                     ARAAVATGVSGVFMETHPDPSKALSDGPNAWPLGLM"
BASE COUNT          506 a          893 c          810 g          412 t
ORIGIN      
        1 cgcacctggt cgatgatgtg gaccacgccc cgggcctcgt ccgcccacag gctgcggtag
       61 tgtttttcgc cgactttcat tcgaaaatta taccgggggg ctgctcgagg agttgagcgg
      121 cggcttcgcc gtctgctcca acgatcgccc cgcttccatt cttttcccgt aaaatatgta
      181 tttccagcac ggagttcgtg atgaccaagt acgtcttcgt gaccggtggc gtcgtcagca
      241 gcctcggcaa gggcatcgct gccgcctccc tcgccgcgat cctcgaatcg cgcaagatca
      301 aagtctccct cgtcaagctc gatccctaca tcaacgtcga tccggggacg atgtcgccgt
      361 tccagcacgg cgaggtgttc gtgaccgagg acggcgccga gaccgacctg gacctcggcc
      421 actacgagcg cttctcgtcg acccgcatga ccaaggccaa caatttcacc accggccaga
      481 tctacgagcg cgtcatcaac aaggagcgcc gcggcgacta cctcggcggc acggtgcagg
      541 tgatcccgca catcaccgac gagatcaagc tgtccatcaa ggccgcgtgc aaggacgccg
      601 aggtcggcat catcgagatc ggcggcaccg tcggcgacat cgagtcgctg ccgttcctcg
      661 aggcgatccg ccagatgggg atcgaggagg gccgcaccaa cacctgctac atccacttga
      721 cgctcgtgcc ctacctcgac gccgtgggcg agatcaagac gaagcccacg cagcactcgg
      781 tgaaggagct gcgcggcatc ggcatccagc ccgacatcgt gatgtgccgc tcggaagtgc
      841 cgatccccga gagcgaccgc cgcaagatcg cgctcttcac caacgtcgac gtgcgctcgg
      901 tgatctcggc gtacgacatg gattcgatct acaagattcc cgtggcgctg cacaagcagg
      961 gcctcgacga gatcgtgtgc cgcaagctcg acctcgcggc ctatcccgcg gacctcacga
     1021 tgtgggagaa catcgtcgac gcgctcgaga accccgagca tcaagtcgac atcgcgatgg
     1081 tcggcaagta catcgacctc accgactcgt acaagtcgct caccgaggcg ctgatccacg
     1141 ccggcatcca cacgcgcacc aaggtgaaga tccactacat cgactccgag cagatcgagg
     1201 ccaagggcat cgagacgctg aaggacatgg acgcggtgct ggtcccgggc ggcttcggca
     1261 agcgcggcat cgagggcaag atcgcggcgg tgaagtacgc gcgcgagaac aaggtcccgt
     1321 acctgggcat ctgcctcggc atgcagatcg ccgcgatcga gatcgcgcgc gacatttgcg
     1381 gcctcgaggg cgccaactcc accgagttcg atcccgacac gccgcacccg gtgatcgcgc
     1441 tcatcaccga gtggcagaac cacgacggca aggtcgaaag gcgcgacgag aagtcctacc
     1501 tgggcggcac catgcgcctg ggcgcgcagc cggcggcggt gaagaagggc acgctcgcgc
     1561 accgcatcta cggcaaggac acggtcgcgg agcgccatcg ccatcgctac gaggtgaaca
     1621 accactacgt gggccggctc gaagccgcgg ggctgaacgt cagcgcgaag accaaggccg
     1681 aagacctcac cgagatcgcc gagctcgcgg accatccgtg gttcgtcggc gtgcagtacc
     1741 accccgagtt cacctcgacg ccgcgcgaag gccacccgct cttcaagtcg ttcatcaacg
     1801 cggcgctcga gcagcacaag cttcgcgtgc cggagcccgc gctcaaggtc gtcacgaaat
     1861 gaagctctgc ggtttcgaag cggggctgga caagcccttc ttcctcatcg ccggcccttg
     1921 cgtcgtcgag agcgaggagc tccagatgga tgtcgcgggg aagctgaagg agatgacggc
     1981 cgagctcggc attcccttca tcttcaagtc gtcctacgac aaggcgaacc gcagctccgg
     2041 gaaatccttc cgcgggccgg gaatggagaa gggcctcgag atcctcgcca aggtgaggaa
     2101 gaccctcggc gtgccgatcc tcaccgacgt gcacaccgag gaggaagtgc cgaccgtggc
     2161 ggcgatcgtc gacgtgctgc agactccggc gttcctctgc cgccagaccg atttcatcca
     2221 cgccgtcgcc cgcgcgggca agcccgtcaa catcaagaag ggccagttcc tcgcgccgtc
     2281 ggacatgaag aacgtggtcg acaaggcgcg cgaggcgagc ggcgccgaca acatcatggt
     2341 gtgcgagcgc ggcgcttcct tcggctacaa caacctcgtc tccgacatgc gctcgctcgc
     2401 gatcatgcgc gacaccggtt gcccggtcgt cttcgacgcg acccattccg tgcagcttcc
     2461 cggcgggcag ggcacgtctt ccggcgggca gcgcgaattc gtcccggtgc tcgcgcgcgc
     2521 cgcggtggcc acaggcgtgt cgggcgtctt catggagacc catcccgatc cgtcgaaggc
     2581 gctctccgac ggccccaacg cgtggccgct cggcctcatg c
//
LOCUS       DASVII010000416         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_522384,
            whole genome shotgun sequence.
ACCESSION   DASVII010000416 DASVII010000000
VERSION     DASVII010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522384"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VF309_05455"
     CDS             <1..702
                     /locus_tag="VF309_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466067.1"
                     /translation="KVLQADPRPPSMLDSALPKGFDSVVARALAKKADERYQTAREFQ
                     AAMLQALQGKNPAATATAMRTQRSAADETAARRAQVLAIDPAALAEVERSLSRHVGPL
                     AKILVKRTQGEAGSIGQFFRALAENIPDADEHKAFMKKMDAVKASLADPPKAEPEPDA
                     AVKTVPSGAQTVFTPEVLAAAEKRLASYVGPLARVLIKEAAGQSGNIRELYRQLAAHI
                     DEEDERQAFLASLPG"
     gene            719..1285
                     /locus_tag="VF309_05460"
     CDS             719..1285
                     /locus_tag="VF309_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7466068.1"
                     /translation="MTTIVVVRKGGNAVIAADSLTTFGTTRLAPAYDRHPQKITTYGD
                     SFLGVAGSAAHQLVLENLLVRTPNLGFHGKAAIYDSFRKLHPLLKDEAFLNPKEEEDD
                     PYESSQMTVMIANPSGIFAVYSMREVFEFDRFWAIGSGRDFALGAMFTVYEKTPTVAA
                     IAETGVLAGAEFDTGTAAPIDLHEVILK"
     gene            1357..1731
                     /locus_tag="VF309_05465"
     CDS             1357..1731
                     /locus_tag="VF309_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX7466069.1"
                     /translation="MRLDKWLWAARFYKTRALAVAAIEAGHATLNGERAKPAKLVKVG
                     DAIGVRRAPFEHAIVVKALSDKRGPAAAAALLYEETAESRARREALAARLKAEPQPRF
                     KGRPTKKTRRDYEKWLRTGDDE"
BASE COUNT          341 a          654 c          636 g          266 t
ORIGIN      
        1 aaggtgctgc aggcggatcc gcggccgccg tcgatgctcg actccgcgtt gccgaaggga
       61 ttcgacagcg tcgtcgcgcg cgcgctggcg aagaaggccg acgagcgcta ccagacggct
      121 cgcgaattcc aggcggcgat gctgcaggcg ctgcagggca agaatcccgc ggcgacggcg
      181 accgcgatgc gcacgcagcg atcggcggcg gatgagacgg cggcgcgcag ggcccaggtg
      241 ctcgcgatcg acccggcggc gctcgcggag gtggagcgct cgctctcgcg ccacgtgggg
      301 cccctcgcga agatcctagt gaagcgcacc cagggcgaag ccgggagcat cgggcaattc
      361 ttccgcgcgc tcgcggagaa catccccgac gccgacgagc acaaggcgtt catgaagaag
      421 atggacgcgg tgaaggcgag cctcgcggac ccgccgaagg ccgagcccga gcccgatgcc
      481 gccgtgaaga ccgtgccctc cggcgcgcag acggtgttca cgccggaagt cctcgccgcc
      541 gcggagaagc gcctcgcgag ctatgtcggg ccgctcgcgc gcgtgctgat caaggaggcg
      601 gccggccaat cgggcaatat cagggagctc taccgccagc tcgccgccca tatcgacgag
      661 gaagacgagc ggcaggcgtt cctcgcgtcc cttcccggct aggcctgaaa gacaactcat
      721 gaccaccatc gtcgtcgtcc gcaagggcgg caacgccgtc atcgcggcgg attccctcac
      781 cactttcggc acgacgcgcc tcgcgccggc atacgatcgc catccgcaga agataacgac
      841 ctacggcgac tcgttcctcg gcgtggcggg cagcgccgcg caccagctgg tgctcgagaa
      901 cctcctcgtg cgcacgccga acctcggctt ccacggcaag gccgcgatct acgacagctt
      961 ccgcaagctc cacccgctgc tcaaggatga ggccttcctc aatccgaagg aggaagaaga
     1021 cgatccctac gagtcgagcc agatgacggt gatgatcgcc aatcccagcg gcatcttcgc
     1081 ggtgtactcg atgcgcgaag tgttcgagtt cgaccgcttc tgggccatcg gctcgggccg
     1141 cgacttcgcc ctgggcgcga tgttcaccgt ctacgagaag acgcccaccg tggccgcgat
     1201 cgccgaaacc ggcgtcctcg cgggcgccga gttcgacacc ggcaccgccg cacccatcga
     1261 cttgcacgaa gtgatcctga aataaactgg tgtcagagac caatttccgc gcacgcaccc
     1321 acgacttgga aattggtctc tgacaccagt ttattcatga gactcgacaa atggctgtgg
     1381 gcggcgcgct tctacaagac gcgcgcgctg gcggtcgcgg cgatcgaggc ggggcacgcc
     1441 acgctgaacg gcgagcgcgc caagcccgcc aagctcgtga aggtgggtga tgcgatcggg
     1501 gtgcgccgcg cgccgttcga gcacgcaatc gtcgtgaagg cgctttcgga caagcgcggg
     1561 cccgccgcgg cggcggcgct cctctacgag gagaccgccg agagccgggc gcgccgcgag
     1621 gcgctcgccg cgcgcctgaa ggccgagccg cagccgcgct tcaaggggcg tcccaccaag
     1681 aagacgcggc gcgactacga gaagtggctt cgcacgggcg acgacgaatg agcccacccg
     1741 gttgaatccc gccgacacga tcgaaggact cgccatcgcg caagccatgc gcgatgtgcc
     1801 gttgctctac gcggcggccg agatcgcgca caccctcggc ttcgtcgtgc tcgtgggcgc
     1861 gacgctcatg ttcgacttgc gggtgctcgg cctttcc
//
LOCUS       DASVII010000417         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_379020,
            whole genome shotgun sequence.
ACCESSION   DASVII010000417 DASVII010000000
VERSION     DASVII010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379020"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VF309_05470"
     CDS             complement(<1..200)
                     /locus_tag="VF309_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466070.1"
                     /translation="MRLSLLLGLVVVVCAAARAGEQDACAECKNSALADASQCQAIAA
                     PDPALRDRCDKDFAQATRACAA"
     gene            complement(219..1571)
                     /gene="ffh"
                     /locus_tag="VF309_05475"
     CDS             complement(219..1571)
                     /gene="ffh"
                     /locus_tag="VF309_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7466071.1"
                     /translation="MLDALTQRLSSVVKTLRGHSRLTEDNVQEMLREVRLALLDADAG
                     VPVVKDFVARVKEKSLGQEVAGSLTPGQALVGIVHQELTALMGGASVPLDFATTPPAI
                     ILLAGLQGSGKTTSAGKLARLIKATTKKKVLLVSTDVYRPAAIDQLATLAKALDIEMY
                     PSDPSQKPVDIARAAVDHAKRHFHDVLIVDTAGRLAIDEAMMDEVRQLHAALDPIETL
                     FVVDSMQGQDAVNVARAFSDALPLTGIVLTKLDGDSRGGAALSVRHVTGKPIKFVGVS
                     EKMDGLEAFHPERMASRILGMGDILSLVEEAHAKVDAKEAQKLADKFKKGKDFDLEDF
                     KSQLTQMKKMGGIASLSDKLPAQLTNMAQASPELQEKALKRTEAIIDSMTPLERRKPE
                     LLKASRKRRIASGAGVQVQEVNRLLAQFEQMRKMMKMMRGGNLGKMLRNMKGMLPGMR
                     "
     gene            1616..2446
                     /gene="ccsA"
                     /locus_tag="VF309_05480"
     CDS             1616..2446
                     /gene="ccsA"
                     /locus_tag="VF309_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7466072.1"
                     /translation="MPDLLLYFSTATCWLLLSGVAWRTSRPVVAAGPEPRADLQLENV
                     LVPVALVLHAMLLYRRVIVPGGLDLGVANAVSMLVWLTVMIYWLGGLAYRGLTGILGL
                     MSPIAFTAVLLQAVVTSGHVVTYGGDPLFALHFAIAMLAYSLFIVATVHAVVMLAEEK
                     WLHRGVLPPFVRRLPPLLEMEALLFRILLAAFVLLTLTVVSGVFFSEQLFGKPLTFSQ
                     KTVFAIISWFIFGGLLAGHHFRGWRGRTAVHWTLAGFTALLLAYVGSKVVLELVLQRG
                     "
     gene            2462..>3727
                     /locus_tag="VF309_05485"
     CDS             2462..>3727
                     /locus_tag="VF309_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX7466073.1"
                     /translation="MTDNIDIGWLFVALAGLCACSAFFAMSETCLMALNRYRLKHLIR
                     EGSRGARLAGSLLDRTDELLAFILAGNTLINAAITILVAETCRRLLGTGDYVLAVATL
                     SAGFFILVFAEILPKVIGATYAEPLALGAAYLLAPLVKFTRWPMWFVNVFVKAILNVL
                     RIRPRNSDKQHLSMAELRTLVLEGGKFIPKKHQSIFLNLFELEDMTVDDTMTPRGQIE
                     HLDLKDDVEQIRNALATAHHTRLVVTEGGGLDNVVGILHVRKVVNATRREQIDVEGLR
                     AIMREPYYVPEGTALLTQLSNFQENQRHMGLVVDEYGEILGLVTMKDILEEIVGEFTS
                     QAIGSAALFHIESDGSVIADGTCSLRVLNRKAGFTFPLDGPKTINGLLLEQLEDIPES
                     GTSLIVAEHPVEILQVQNRMVKVVRILPTI"
BASE COUNT          554 a         1284 c         1216 g          673 t
ORIGIN      
        1 ctcgcggcgc atgcgcgcgt cgcctgcgcg aagtccttgt cgcaccggtc gcgcaacgcg
       61 ggatcgggag ccgcgatcgc ctggcactgg ctcgcgtcgg cgagggcgga gttcttgcac
      121 tcggcgcatg cgtcctgttc gcccgcacgc gcggcggcgc acaccacgac gaccaggccc
      181 agcaaaagac tcagccgcat cgcgaccccc caccggtttc aacgcatgcc gggaagcatc
      241 cccttcatgt tgcgcagcat cttgccgaga ttgccgccgc gcatcatctt catcatcttg
      301 cgcatctgct cgaactgcgc gaggaggcga ttgacctcct gcacctgcac gcccgcgccc
      361 gaggcgatgc ggcgcttgcg cgaagccttg aggagctcgg gcttgcgccg ctcgagcggc
      421 gtcatcgaat cgatgatcgc ctcggtgcgc ttcagcgcct tctcctgcag ctcgggcgag
      481 gcctgcgcca tgttggtgag ctgcgccggc agcttgtccg aaaggctcgc gatgccgccc
      541 atcttcttca tctgcgtgag ctgcgacttg aagtcctcga ggtcgaagtc cttgcctttc
      601 ttgaacttgt cggcgagctt ctgcgcttcc ttcgcatcga ccttggcgtg cgcctcctcc
      661 acgagcgaga ggatgtcgcc catgccgaga atgcgcgacg ccatgcgctc ggggtggaag
      721 gcctcgaggc cgtccatctt ctccgagacg ccgacgaact tgatcggctt gccggtgacg
      781 tggcggaccg agagcgccgc gccgccgcgt gaatcgccgt cgagcttggt gagcacgatg
      841 ccggtgagcg gcaacgcgtc gctgaacgcg cgcgcgacgt tcaccgcatc ctggccctgc
      901 atcgagtcga ccacgaagag cgtctcgatc ggatcgagcg ccgcgtggag ctgccggacc
      961 tcgtccatca tcgcttcgtc gatcgcgagc cgccccgccg tgtcgacgat gagcacgtcg
     1021 tggaagtggc gcttcgcgtg atccaccgcg gcgcgcgcga tgtcgacggg cttctgcgaa
     1081 gggtccgacg ggtacatctc gatgtcgagc gccttggcga gggtggcgag ctggtcgatg
     1141 gccgcggggc ggtacacgtc cgtcgagacg agcagcacct tcttcttcgt cgtggccttg
     1201 atgaggcgcg cgagcttgcc cgcgctggtg gtcttgcccg atccctgcaa gccggcgagg
     1261 aggatgatcg ccggcggcgt ggtcgcgaag tcgagcggca cgctcgcccc gcccatgagc
     1321 gcggtgagct cctggtggac gatgccgacg agcgcctgcc ccggcgtgag gcttccggcg
     1381 acctcctggc cgagcgactt ctccttcacc ctggcgacga agtccttcac caccggcacg
     1441 cccgcgtcgg cgtcgaggag cgccaggcga acctcgcgca gcatttcctg gacgttgtcc
     1501 tcggtaaggc gcgagtgtcc gcgcagggtc ttcacgaccg aggagaggcg ttgcgtcagg
     1561 gcgtcgagca tggggaattg gtaaactgat gagctatcgc gttgacggca ccgaaatgcc
     1621 tgatttgctt ttgtatttta gcaccgcaac ttgctggctc ctgctctcgg gcgttgcgtg
     1681 gcgcacatcg cgcccggtcg tggcggcggg acccgagccg cgcgccgacc tgcagctcga
     1741 gaacgtgctg gtgccggtcg cgctcgtcct gcacgcgatg ctgctctatc gccgcgtgat
     1801 cgtcccgggc ggcctcgacc tgggagtcgc caacgcggtg tcgatgctcg tgtggctcac
     1861 cgtgatgatc tactggctgg gaggcctcgc ctatcgcggc ctcacgggaa tcctcggcct
     1921 catgtcgcca atcgcgttca cggcggtgct gctgcaggcg gtggtcacgt cgggccacgt
     1981 ggtcacctac ggcggggacc cgctcttcgc cctgcacttc gcgatcgcga tgctcgccta
     2041 cagcctcttc atcgtcgcga ccgtgcatgc ggtggtgatg ctcgccgagg agaagtggct
     2101 gcatcgcggc gtgctgccgc ccttcgtgag gcgcctgccg ccgctgctcg agatggaggc
     2161 gctgctcttc cgcatcctgc tcgcggcctt cgtgctcctc acgctcaccg tggtgagcgg
     2221 cgtcttcttc tcggagcagc tcttcggcaa gccgctcacc ttctcgcaga agaccgtgtt
     2281 cgcgatcatc tcgtggttca tcttcggcgg gctcctcgcg ggacaccact tccgcggatg
     2341 gcggggccgc accgccgtgc actggacgct cgcgggattc acggcgctcc tgctcgccta
     2401 cgtgggcagc aaggtggtgc tcgagctcgt gctgcagcgc ggctagcccg cgcccctcgc
     2461 cgtgaccgac aacatcgaca tcggctggct cttcgtcgcg ctcgccggtc tgtgcgcctg
     2521 ctcggcgttc ttcgcgatgt cggagacgtg cctcatggcg ctcaaccggt accgcctgaa
     2581 gcatctcatc cgcgaaggca gccgcggcgc gcggctcgcc ggatccctcc tcgaccgcac
     2641 cgacgagctg ctggcattca tcctcgcggg caatacgctc atcaacgccg ccatcacgat
     2701 cctcgtcgcc gagacctgcc ggcgcctgct cggcaccggc gactacgtcc tcgcggtggc
     2761 gacgctctcg gcgggcttct tcatcctggt gttcgcggag atcctgccga aggtcatcgg
     2821 cgccacctac gccgagcccc tcgcgctcgg cgccgcgtac ctcctcgcgc cgctcgtcaa
     2881 gttcacgcga tggccgatgt ggttcgtgaa cgtcttcgtg aaggcgatcc tcaacgtcct
     2941 gcgcatcagg ccgcgcaatt ccgacaagca gcatctgagc atggcggagc tgcgcaccct
     3001 cgtgctcgag ggcgggaagt tcattccgaa gaagcaccag tcgatcttcc tcaacctctt
     3061 cgagctcgag gacatgacgg tcgacgacac gatgacgccg cgcggccaga tcgagcacct
     3121 cgacctgaag gacgacgtcg agcagatccg caacgcgctc gcgaccgcgc accacacgcg
     3181 cctcgtcgtg accgagggcg gcggcctcga caacgtggtc ggcatcctgc acgtgcgcaa
     3241 ggtcgtcaac gccacgcgcc gcgagcagat cgacgtcgag gggctgcgcg cgatcatgag
     3301 ggagccctac tacgtccccg aggggacggc gctgctcacg cagctcagca atttccagga
     3361 gaaccagcgc cacatgggcc tcgtggtgga cgaatacggc gagattctcg ggctggtcac
     3421 gatgaaggac atcctcgagg agatcgtcgg cgagttcacg agccaggcga tcggcagcgc
     3481 cgccctcttc cacatcgaga gcgacggcag cgtgatcgcc gacgggacgt gctcgctgcg
     3541 cgtgctgaac cgcaaggcgg gcttcacctt cccgctcgac gggccgaaga ccatcaacgg
     3601 ccttctcctc gagcagctcg aggacattcc ggagtcgggc acgagcctca tcgtcgccga
     3661 gcacccggtc gagatcctgc aggtgcagaa ccgaatggtg aaggtggtgc ggatactgcc
     3721 gacgatc
//
LOCUS       DASVII010000418         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_702281,
            whole genome shotgun sequence.
ACCESSION   DASVII010000418 DASVII010000000
VERSION     DASVII010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702281"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..777
                     /locus_tag="VF309_05490"
     CDS             4..777
                     /locus_tag="VF309_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX7466074.1"
                     /translation="MPRECRFGGAARGNVMRGSIKNLLTSVAGVDTNAVTNLRTAVGG
                     REEESLDDAKIRVPRAIRSRCRAVTADDFEYLAMQAANIKRALALPLTHPGFPGVKVP
                     GVVTVVVVPDSEAPNPIPSEGTLRSVCAYLNQARMLTTELYVVRPTYHQVRVTAEIVA
                     ENTADLLEVKTTVEQALLDYFHPLRGGESGDGWPFGGDIYFSRVYQRVSVRGAQRIER
                     MVISVDGCEAPDCTNIPVCDGDLLYSVDHDVTVTYDFGG"
     gene            781..>1515
                     /locus_tag="VF309_05495"
     CDS             781..>1515
                     /locus_tag="VF309_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX7466075.1"
                     /translation="MTPSFLPPLLQPPHDPTWLALDAQQGWRAATLDTAELSPGAGAL
                     TLAVAAGDRSMVEESGSLGGLVPPRNVAVDGDGGVMLLDLLDCVLKRYDPCSCTFLRI
                     PGVGGKGAGPRQFADPHAIAIAGGNLFVADTGNRRVSVFTLNGYQLRSTLKPPAAAGL
                     AQPWTPYALATDAEGRLYVSDPANGCVHRFSRARRWEMSISGLGTVHQLAVDCVGQLY
                     VGVAGEEPVVRLYDAHGRQVGNATRAG"
BASE COUNT          238 a          477 c          549 g          251 t
ORIGIN      
        1 atcatgccac gcgagtgtcg cttcggcggc gctgctcgcg ggaatgtcat gcgtggcagc
       61 atcaagaacc tgctcacatc ggtcgcgggt gtggatacga acgcggtgac gaatctgcgc
      121 accgccgtgg gcgggcgcga ggaagagagc ctcgacgacg cgaagatccg ggtgccgcgc
      181 gccattcgca gccggtgccg cgccgtgacg gccgacgact tcgagtatct cgcgatgcag
      241 gcggcgaaca tcaagcgcgc gctcgcgctg ccgctcacgc atccgggttt ccccggtgtg
      301 aaggtccccg gcgtggtgac ggtggtggtg gtgcccgaca gcgaggcgcc caatcccatt
      361 cccagcgagg ggacactgcg gagtgtatgc gcctacctca atcaggcgcg gatgctcacc
      421 accgagctat acgtcgtccg cccgacatac caccaggtgc gggtgacggc ggagatcgtg
      481 gcggaaaaca ccgcggatct gctcgaggtc aagacgacgg tcgagcaggc attgctcgac
      541 tatttccatc cgttgcgtgg cggcgagtcg ggcgacggat ggccgttcgg cggtgacatc
      601 tacttctcgc gcgtctacca gcgggtgtcg gtgagggggg cgcagcgcat cgagcgaatg
      661 gtgatctcgg tggatggatg cgaggcgccc gactgcacca acattcccgt gtgcgacggc
      721 gacctgctgt actccgtcga tcacgatgtg accgtcacct acgacttcgg gggctaggcg
      781 atgaccccga gctttctccc gccgctgttg caacccccgc acgatcccac ctggctggcg
      841 ctcgacgcgc agcaggggtg gcgcgccgcc acgctggata cggcggagct ctcgcccggc
      901 gcaggggcgt tgacgttggc ggttgccgcc ggcgatcgct cgatggtgga ggagagtggc
      961 agcctgggtg gactcgtgcc gccgcggaac gtcgcggtgg acggggacgg cggtgtcatg
     1021 ctgctcgatc tgctcgactg cgtgctcaag cgatacgacc cgtgcagctg caccttcttg
     1081 cgcattcccg gtgttggagg aaagggcgcg ggcccgcgcc aattcgccga cccgcacgcg
     1141 atcgccatcg ccggcggcaa cctgttcgtc gccgacacgg ggaacaggcg ggtgagcgtc
     1201 ttcacattga atggctacca gctacgcagc acgttgaagc cgcccgccgc cgcaggcctc
     1261 gcgcagccgt ggacgccata cgcgctcgcg acggacgccg agggacggct ctacgtgagc
     1321 gatccggcga atggctgtgt gcatcgcttc agccgcgcgc gccgttggga gatgagcatc
     1381 agcgggctgg gcacggtgca tcagttggcg gtggactgcg tgggtcagct ctacgtgggc
     1441 gtggcgggtg aggagcccgt ggtgcgcctt tacgatgcgc atggacgtca ggtgggcaat
     1501 gccacgcgcg cgggc
//
LOCUS       DASVII010000419         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_450404,
            whole genome shotgun sequence.
ACCESSION   DASVII010000419 DASVII010000000
VERSION     DASVII010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450404"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VF309_05500"
     CDS             <1..506
                     /locus_tag="VF309_05500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7466076.1"
                     /translation="LVYDGGLLKVHRDQVRLHDGTLAWREYVKHPGAVMVLAFVDAET
                     ILLERQFRYPKNRHFIEIPAGKLEPDEPALATAKRELIEECGFEASEWWKIATLDPSI
                     GYSTEVIHLYGARGLKHVGAALDAGEHLQVFEAKVADAIEWVRQGVITDTKTTLALLW
                     WDEWGRP"
     gene            503..>1951
                     /gene="glpD"
                     /locus_tag="VF309_05505"
     CDS             503..>1951
                     /gene="glpD"
                     /locus_tag="VF309_05505"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX7466077.1"
                     /translation="MIDLLVIGGGINGVGIARDAAGRGFSVTLVEQGDLASATSSWSS
                     KLIHGGLRYLEQYEFHLVREALQEREVLLRIAPHIIRPLMFVLPYDASMRPMWMIRAG
                     LWLYDHIGGTITLPRSKALTFPHMEFSAGLRPDIRAGFVYSDCRVDDSRLTVLNAVSA
                     RGKGATILTRTRFEGARREEGAWRVTLAPRSGAPMEVRARAIVNAAGPWVVDVLGHIE
                     GERIRERVRLVKGSHIIVPKVHSQGHALILQNPDKRVVFVIPYEHDFSLLGTTDVAVS
                     RVEEAARITEEESDYLIAAANRFLARPIAAADIVHSYAGVRPLYDDGSDNPSEITRDY
                     VIKVDHDHKTVPLLTVFGGKITTYRRLAEEALEHLAPFFANLKAPWTATEELPGGDVG
                     NFNAYRDAMHSRYPGLGRELVEGVVRRHGTRAPTILGDARRPEALGRGFGAGLTQREI
                     EFLVTDEWAETAEDVLWRRTKCGLHMTREQADA"
BASE COUNT          321 a          677 c          662 g          291 t
ORIGIN      
        1 aactcgtcta cgacggcggg ctcctcaagg tccaccgcga ccaggtgcgc ctgcacgacg
       61 ggacgctcgc gtggcgcgaa tacgtgaagc acccgggcgc ggtgatggtg ctcgcgttcg
      121 tggacgccga gaccatcctc ctcgagcgcc agttccgcta tccgaagaat cgccatttca
      181 tcgagatccc cgcgggcaag ctcgagccgg acgagccggc gcttgccacg gcgaagcgcg
      241 agctcatcga ggagtgcggt ttcgaggcct ccgagtggtg gaagatcgcg accctcgacc
      301 cctcgatcgg ctactcgacg gaagtgatcc atctctacgg cgcgcgcggc ctgaagcacg
      361 tgggcgccgc cctcgacgcg ggcgagcacc tgcaggtgtt cgaggcgaag gtggccgacg
      421 cgatcgaatg ggtgcgccag ggcgtcatca ccgataccaa gacgacgctc gcactcctgt
      481 ggtgggacga atggggacga ccgtgatcga cctcctggtc atcgggggcg gcatcaacgg
      541 agtgggaatc gcccgcgacg cggccggccg cggcttctcg gtgacgctgg tcgagcaggg
      601 cgacctcgcc tcggcgacga gctcgtggag ctcgaagctg atccacggcg ggctgcgcta
      661 tctcgagcag tacgagttcc acctggtgcg cgaggccctc caggagcgcg aggtgctgct
      721 gcgaatcgcg ccccacatca tccggcccct catgttcgtg ctgccctacg acgcgagcat
      781 gcggcccatg tggatgatcc gcgcgggact gtggctctac gatcacatcg gcggcaccat
      841 caccctgccg cgctccaagg cgctcacctt tccgcacatg gaattcagcg ccggcctgag
      901 gcccgacatc cgcgcgggat tcgtgtattc cgattgccgc gtggacgatt cgcgcctcac
      961 ggtcctcaac gcggtttcgg cgcgcggcaa gggggccacg atcctcacgc gcacgcgctt
     1021 cgagggcgcg cggcgcgagg agggcgcctg gcgcgtgacg ctcgccccgc gctcgggggc
     1081 gccgatggaa gtgcgtgcga gggcgatcgt gaatgccgcc gggccctggg tcgtggacgt
     1141 gctgggccac atcgagggcg aacgcatccg cgagcgcgtg cgcctcgtga agggcagcca
     1201 catcatcgtc cccaaggtcc actcgcaggg gcacgcgctc atcctgcaga atcccgacaa
     1261 gcgcgtggtg ttcgtgattc cctacgagca cgacttcagc ctcctcggca ccaccgacgt
     1321 tgcggtgtcg cgcgtggagg aggcggcgcg catcaccgag gaggagagcg actatctcat
     1381 cgccgcggcc aatcgcttcc tcgccaggcc gatcgccgcc gccgacatcg tgcactcgta
     1441 cgcgggcgtg cgtcccctct acgacgacgg ttccgacaac ccgtcggaaa tcacgcgcga
     1501 ctatgtgatc aaggtggacc acgaccacaa gacggtgccg ctcctcaccg tcttcggcgg
     1561 aaagatcacg acgtaccggc gactcgcgga agaagcgctc gagcacctcg cgcctttctt
     1621 cgcgaacctc aaggcgccct ggaccgcgac cgaggagctt cccggaggcg acgtcggcaa
     1681 cttcaacgcg tatcgcgacg ccatgcactc gcgctatccg ggactgggcc gcgagctcgt
     1741 ggagggcgtc gtgcgccgcc acggcacgcg cgcgccgacg attctcggcg acgcgcgccg
     1801 ccccgaggcg ctcggcaggg gcttcggcgc gggcctcacc cagcgcgaga tcgaattcct
     1861 cgtcaccgac gagtgggccg agaccgcgga ggacgtcctc tggcgccgca ccaagtgcgg
     1921 attgcacatg acgcgcgagc aggcggacgc c
//
LOCUS       DASVII010000420         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_558770,
            whole genome shotgun sequence.
ACCESSION   DASVII010000420 DASVII010000000
VERSION     DASVII010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558770"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VF309_05510"
     CDS             <1..236
                     /locus_tag="VF309_05510"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX7466078.1"
                     /translation="RKRYFKELAGPLATAAVEKLHDLAEAKRNLTLVYASRNEERNNA
                     TVLKELLEGSRKPPSSVGRSAVGGGRIRKARKQ"
     gene            316..>1509
                     /gene="ligA"
                     /locus_tag="VF309_05515"
     CDS             316..>1509
                     /gene="ligA"
                     /locus_tag="VF309_05515"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7466079.1"
                     /translation="MSAALDKKIDSLRETIRHHEYRYYVLDDPEITDAGFDKLINELK
                     ALEKEHPERVTPNSPTQRVGGKPREGFVKARHSSPILSLDNAYTEEELRDWERRVHEL
                     GGRSDVEYVCELKLDGMSLALRYTGGQLERGITRGDGNEGEDVTSNVRTVRSVPLSIP
                     KEKLKKAGIPLEFEVRGEMLMPLAGFRKMNEEREQQGLSQFANPRNATAGTVRQLEPS
                     ITAQRRLDYFPYMLLKDGRTYFDRHSETLAALEAAGFKVNPHHKLAKNLDEVWKFIQN
                     WEGKRDSLPYEIDGIVIKVDRTALQQELGFTGKAPRWAIAFKYAARGAVTQIEDILVQ
                     VGRTGKLTPVAALTPVPIGGTTVSRATLHNMDEIDRLGVRIGDWVEVERGGDVIPKVV
                     KVTEDK"
BASE COUNT          335 a          473 c          471 g          230 t
ORIGIN      
        1 tccgcaagcg ctatttcaag gagctggctg gtcccctagc gaccgccgcg gtcgagaagc
       61 tgcacgacct ggccgaagca aagcgaaacc tcacgctggt ctatgcttcc cggaatgaag
      121 aacgcaacaa cgccacggtg ctgaaggaat tgctcgaagg atcccggaaa ccgccgtcca
      181 gcgtgggacg cagcgcggtt ggcggaggcc ggatcagaaa agcgagaaag cagtagccgg
      241 cagtcagcag tcagttctct tgtgctgacg gccgatggct gaacgctgac ggctcagaac
      301 tataatcttc cctgcatgtc cgccgccctc gacaagaaga tcgactccct gcgcgagaca
      361 atccgccacc acgaataccg ctactacgtg ctcgacgatc cggaaatcac cgacgccggc
      421 ttcgacaagc tcataaacga gctcaaggcg ctggagaaag agcacccgga gcgcgtcacg
      481 cccaactctc cgacccagcg cgtcgggggc aagccgcgcg agggattcgt gaaggcccgg
      541 cattcctcgc ccatactctc gctcgacaac gcttacacgg aagaagaact gcgcgactgg
      601 gagcggcgcg tgcatgaact gggcgggcgg agcgacgtcg aatatgtgtg cgagctaaaa
      661 ctcgatggca tgtccctggc gctgcgctat accggcggcc agctcgaacg cggcatcacg
      721 cgcggcgacg gcaacgaagg cgaagacgtc acatcgaacg tgcgtacggt gcggtccgtc
      781 ccgctctcga tccccaaaga gaagctgaag aaggccggca ttccgctcga gttcgaagtg
      841 cgcggcgaga tgcttatgcc tctcgcgggg ttccgcaaga tgaacgagga gcgcgagcag
      901 caggggcttt cgcagtttgc caacccgcgc aacgccacgg ccggtacggt tcgtcaactg
      961 gagccgagca tcacggcgca gcgccgcctc gactattttc cgtacatgct gctcaaagac
     1021 ggccggacat actttgaccg ccactccgaa acgctggccg ccctcgaggc agccggcttc
     1081 aaagtgaatc cgcaccacaa gctggcgaag aatctggatg aggtctggaa gtttatccag
     1141 aactgggaag gaaagcgcga ttcgctccct tacgaaatcg acggcatcgt catcaaggtg
     1201 gaccgcaccg cgctgcagca ggagctcggc ttcacgggca aggctccgcg ctgggccatc
     1261 gcattcaaat atgcggcgcg cggcgcggtc acgcagatcg aggacatcct ggtgcaggtt
     1321 ggccgcacgg gcaagctgac gccggtggcc gcgctcacgc ccgtcccgat cggcggcacc
     1381 accgtttccc gcgccacgct ccacaacatg gacgagatcg accgcctcgg ggtaagaatc
     1441 ggcgactggg ttgaggtgga gcgcggcggc gacgtgatcc ccaaagtggt caaagtcacg
     1501 gaggataaa
//
LOCUS       DASVII010000421         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_702384,
            whole genome shotgun sequence.
ACCESSION   DASVII010000421 DASVII010000000
VERSION     DASVII010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702384"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..92
                     /locus_tag="VF309_05520"
     CDS             15..92
                     /locus_tag="VF309_05520"
                     /inference="COORDINATES: protein motif:HMM:NF036305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466080.1"
                     /translation="MGTAALEALGEDGDFVPCLHSVGMP"
     gene            110..1351
                     /locus_tag="VF309_05525"
     CDS             110..1351
                     /locus_tag="VF309_05525"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX7466081.1"
                     /translation="MPWPCNADNKYIVHYPETKEIVSFGSGYGGNALLGKKCLALRIA
                     SVMGREQGWLAEHMLILGVESPEGRKHYVTAAFPSACGKTNFAMLIPPPAFKGWKVTT
                     VGDDIAWIKPGKDGSLRAINPEAGFFGVAPGTGWDTNPNCMETIKRDTIFTNVALTPE
                     GDVWWEGMTKEPPAKLTDWTGKEWTPQSGRTAAHANARFTVGAAQCPSIDPQWDDPEG
                     VPIDAFIFGGRRSTTVPLVTEAKSWRDGVYMAATLGSETTAAIVGQVGVVRRDPFAML
                     AFCGYHIADYVAHWLKLGGALKKQPKIYLVNWFRKDANGKFIWPGYGENMRVLKWIVG
                     RVEGNADAVETPLGNVPKSLDMTGLDSIGAERIAAANAVSKAEWQKEMPLHGEMVEGK
                     LAERAPKEMMERFEELKAAFR"
     gene            complement(1344..>1789)
                     /locus_tag="VF309_05530"
     CDS             complement(1344..>1789)
                     /locus_tag="VF309_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7466082.1"
                     /translation="RLLPAGGIYFRLDESDVQRILRHGKTMIGSDGLPHDASPHPRLW
                     GAFPRVLGHYCRELGLFSLETSVHKMTGLTARNFGLRDRGVVKEGAYADLTLFDADKV
                     DEAATYAAPIAPARGIDAVIVNGAVVWRDGRPTGARPGRVLARLT"
BASE COUNT          310 a          598 c          623 g          258 t
ORIGIN      
        1 ggatcatgac ccgcatgggc accgccgccc tcgaggcgct cggcgaggac ggcgacttcg
       61 tcccctgcct gcactcggtc ggcatgccct gaccgacggc atcgaggacg tgccgtggcc
      121 gtgcaacgcc gacaacaagt acatcgtcca ttaccccgag acgaaggaga tcgtctcgtt
      181 cggctcgggc tacggcggca acgcgctcct cggcaagaag tgcctggcgc tgcgcatcgc
      241 atcggtgatg gggcgcgagc agggctggct cgccgagcac atgctgatcc tcggcgtgga
      301 atcgcccgag gggcgcaagc actacgtcac cgcggcgttc ccgagcgcct gcggcaagac
      361 caacttcgcg atgctgatcc cgccgcccgc tttcaagggc tggaaggtga ccacggtcgg
      421 cgacgacatc gcgtggatca agccgggcaa ggacgggtcg ctgcgcgcca tcaatcccga
      481 ggcgggattc ttcggcgtcg cgcccggcac cggctgggac acgaacccca actgcatgga
      541 gacgatcaag cgcgacacca tcttcaccaa cgtcgcgctc acgcccgagg gcgacgtgtg
      601 gtgggaaggc atgacgaagg agccgccggc gaagctcaca gactggaccg gcaaggagtg
      661 gacgccgcaa tcggggcgca ccgcggcgca tgccaacgcg cgcttcaccg tcggcgccgc
      721 gcagtgcccc tcgatcgacc cgcagtggga cgatcccgag ggcgtgccga tcgacgcgtt
      781 catcttcggc gggcggcgct cgacgacggt gccgctcgtc acggaagcga agagctggcg
      841 tgacggggtg tatatggccg ccacgctcgg gtccgagacc accgcggcca tcgtgggcca
      901 ggtgggcgtg gtgcggcgcg atcccttcgc gatgctcgcc ttctgcggtt accacatcgc
      961 cgattacgtc gcccattggc tgaagctcgg cggcgcgctg aagaagcagc cgaagatcta
     1021 tctcgtgaac tggttccgca aggacgccaa cggcaagttc atctggcccg gctacggcga
     1081 gaacatgcgc gtgctcaagt ggatcgtggg gcgtgtcgag gggaatgccg atgccgtcga
     1141 gacgccgctc ggcaacgtgc cgaagtcgct cgacatgacg ggcctcgatt ccatcggcgc
     1201 cgagcgcatc gccgccgcga acgcggtgtc gaaggccgag tggcagaagg agatgccgct
     1261 ccacggcgag atggtcgagg gcaagctcgc cgagcgcgcg ccgaaggaga tgatggagcg
     1321 cttcgaggag ctgaaggcgg ccttcaggta aggcgcgcca gtacccggcc gggccgcgcg
     1381 cccgtcggcc ggccgtcgcg ccagacgacg gcgccattga cgatcaccgc atcgatgccg
     1441 cgagcggggg cgatcggcgc ggcgtatgtg gcagcctcgt ccaccttgtc cgcgtcgaac
     1501 agcgtcaagt cggcataggc cccctccttc acgacgccgc ggtccctgag gccgaaattc
     1561 ctcgccgtga gcccggtcat cttgtgcacc gaggtctcca gtgaaaacaa cccgagctcg
     1621 cggcaatagt ggcccaggac gcgcgggaag gctccccaaa ggcgcggatg cggcgatgcg
     1681 tcgtgcggta ggccgtcgga gccgatcatg gtcttgccgt gccggaggat gcgctgcacg
     1741 tcgctctcgt ccagcctgaa gtagatccct cccgccggaa gcagccgcc
//
LOCUS       DASVII010000422         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_702495,
            whole genome shotgun sequence.
ACCESSION   DASVII010000422 DASVII010000000
VERSION     DASVII010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702495"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1314)
                     /locus_tag="VF309_05535"
     CDS             complement(202..1314)
                     /locus_tag="VF309_05535"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX7466083.1"
                     /translation="MPLEFTSSAALSMGVELELMVLNTHDHNLVRGAPDLLARLDKVE
                     LKGEVKPEITESMIEVNSSVNTRYEELLAELRHIRDAIVVAADRLNVAVAGGGAHPFH
                     KWNEQRIFPTERFQRVSSLYGYLAKQFTVFGQHIHIGVASGDDAVYLTHRLAPYIPHF
                     IALSASSPFSQGEDTSFETSRLHAVAAFPLSGQMPPVRTWDEFNRYFDEMKGYGIVAS
                     MKDFYWDIRPKPEYGTVEIRICDTPLTVDLAAQLAAYAQTLAADLLENRRDDDPLRMY
                     RVYGYNRFQACRFGFEGELVNPFEHTHTRLRDDLLETLERLTPAAAALGTAMPLMSIA
                     ALAARQENDSRWLRERYLETKSLPDVVRMQAARWRE"
     gene            complement(1321..>1869)
                     /locus_tag="VF309_05540"
     CDS             complement(1321..>1869)
                     /locus_tag="VF309_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7466084.1"
                     /translation="MVVAAVGLAILLKLSVILSLLAFGLFTRNDPRNYDLLNVNLAPA
                     SRLLYIVLFVITGASLPLPALVAGGAAGMAIAAARVMGKLVGVLAIAPIGGLRLRQAV
                     GLACALMPMSTIALVLHHDVTRAFPEFGADLTSAFLAMVIVMELVGPIAVQWGLRLAG
                     ESAPGGEIGVAPVRDAARVPSA"
BASE COUNT          302 a          614 c          661 g          292 t
ORIGIN      
        1 atggttggga aatgtggcgc tcccaccact tgttcgccat cgccattcac gagtgcgtca
       61 ctgccggccg ggtcaaactc ttccccgccc tcgctcgctg ttgatcgcac tcttgagtgc
      121 gtgcggtgga taccccttga atcccacgca cggacttgcg gctgttcaat cccacgcgag
      181 cctgcggatg ttgggcagag cctattcccg ccagcgcgcg gcctgcatcc tcacgacgtc
      241 ggggagggac ttggtctcga ggtagcgctc gcgaagccag cgcgagtcgt tctcctggcg
      301 tgccgcgagg gcggcgatcg acatcagcgg catcgcggtg ccgagcgccg ccgccgcggg
      361 cgtgaggcgc tcgagcgtct cgaggagatc gtcgcgcaag cgcgtgtggg tgtgctcgaa
      421 cggattcacc agctcgccct cgaagccgaa gcggcaggcc tggaagcggt tgtagccgta
      481 gacgcggtac atgcgaagcg ggtcgtcgtc gcggcggttc tcgaggagat cggcggcgag
      541 ggtctgcgcg taggcggcga gctgcgccgc gaggtccacc gtgagcggcg tgtcgcagat
      601 gcggatctcg accgtgccgt actcgggctt cgggcggatg tcccagtaga agtccttcat
      661 gctcgcgacg atgccgtagc ccttcatctc gtcgaagtag cggttgaact cgtcccacgt
      721 gcgcaccggc ggcatctgcc ccgaaagcgg aaacgcggcc accgcgtgca ggcgcgaggt
      781 ctcgaaggac gtgtcctcgc cctgcgagaa gggcgaggac gccgacaggg cgatgaaatg
      841 cggaatgtag ggcgcgaggc gatgcgtgag gtacaccgcg tcgtcgccgc tcgcgacgcc
      901 gatgtggatg tgctgcccga agaccgtgaa ctgcttggcg aggtagccgt agaggctcga
      961 cacgcgctgg aagcgctccg tcggaaagat gcgctgctcg ttccacttgt ggaacgggtg
     1021 cgcgcccccg cccgccaccg cgacgttgag ccgatccgcg gcgacgacga tcgcgtcgcg
     1081 gatgtgccgc agctcggcga ggagctcctc gtagcgcgtg ttcaccgagg agttgacctc
     1141 gatcatgctt tcggtgatct cgggcttcac ctcgcccttg agctcgacct tgtcgaggcg
     1201 ggcgagcaga tccggcgcgc cgcgcacgag gttgtggtcg tgcgtgttga gcaccatgag
     1261 ctcgagctcg acgcccatcg agagcgccgc ggaagaggtg aattcgagcg gcatcggggc
     1321 ttacgcgctc ggcacgcggg ccgcgtcgcg caccggcgca acgccgatct ctcccccggg
     1381 cgcgctctcg ccggcgagcc tgagtcccca ctggaccgcg atggggccga cgagctccat
     1441 cacgatcacc atcgcaagga aggccgaggt gaggtccgcg ccgaattccg ggaaggcgcg
     1501 cgtcacgtcg tggtgaagga cgagggcgat ggtcgacatc ggcatcaacg cgcacgcgag
     1561 ccccaccgcc tggcgcagcc tcaagccccc gatcggcgcg atcgcgagca cgcccacgag
     1621 cttgcccatc acgcgcgccg ccgcgatcgc cattcccgcg gcgcctcccg cgacgagcgc
     1681 cggcagcggc aggctcgcgc cggtgatcac gaagagcacg atgtacagga gccggctcgc
     1741 cggcgcgagg ttcacgttga gcaggtcgta gttgcgcggg tcgttgcgcg tgaagagccc
     1801 gaaggcgagg agcgacagga tcaccgagag cttgagcagg atcgcgagcc cgaccgcggc
     1861 caccaccat
//
LOCUS       DASVII010000423         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_379251,
            whole genome shotgun sequence.
ACCESSION   DASVII010000423 DASVII010000000
VERSION     DASVII010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379251"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /gene="pepN"
                     /locus_tag="VF309_05545"
     CDS             complement(<1..1682)
                     /gene="pepN"
                     /locus_tag="VF309_05545"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX7466085.1"
                     /translation="MLARPDTATDTDYMLIDRVVAHEYFHNWTGNRVTCRDWFQLSLK
                     EGLTVYRDQEYSADEYSRPVVRIQEVRDLRNRQFPEDAGPMAHPVRPESYVEINNFYT
                     ATVYDKGAEVVRLYATILGKEGFRRGMDLYFKRHDGQAVTCDDFRAAMAEANQVDLAQ
                     LGLWYSQSGTPVVECRGEYDPRGETYTLHAKQTCPPTPGQAAKKPFVIPLAVGLVDLD
                     GRDLPINIDSKSLFKGARANGSRTAVLALTAAEQSFVFHQVPENPVPSLLRGFSAPVH
                     VRYDYTDSELAHLMAYDSDAFNRWEAGQALAIRILLASIDSVREGRAMEVPPTFIDAM
                     GRVISDGSKDPAFAAEALMLPAEGYLAECMEVADPEAIHIARMRLMREIATKFRTRFE
                     GAFRHFTVAGPYSPDAAASGRRALRNTALSYVTTIDDGTSRALAFLEFRRAENMTDAM
                     AALTCLSNSAGAERERALSMFYEKWKDEALVIDKWFRGQAMSWLPGTLDRVKALTSHP
                     AFDLKNPNRARSLLHTFALENPMHFHAADGRGYQWVAEQVIALDKLNPQVASR"
BASE COUNT          254 a          536 c          612 g          292 t
ORIGIN      
        1 cgcgaggcga cctggggatt cagcttgtcg agcgcgatca cctgctccgc gacccactgg
       61 taccctctgc cgtcggcggc gtggaagtgc atcgggtttt ccagggcgaa ggtgtgcagc
      121 agcgagcgcg cgcgattcgg attcttcagg tcgaacgcag ggtgggaggt gagcgccttc
      181 acgcgatcga gggtgcccgg cagccacgac atcgcctgcc cgcggaacca cttgtcgatc
      241 accagggcct cgtccttcca cttttcgtag aacatcgaca gggcgcgctc gcgctcggcg
      301 cccgccgagt tggagaggca ggtgagcgcg gccatcgcgt cggtcatgtt ctcggcgcgg
      361 cggaactcga ggaacgcgag cgcgcgcgac gtgccgtcgt cgatggtcgt cacgtacgag
      421 agagcggtgt tgcgcagcgc gcgccggccg gacgcggcgg catcgggcga atatggcccg
      481 gccaccgtga aatggcggaa ggcgccctcg aagcgggtgc ggaacttggt cgcgatctcg
      541 cgcatgaggc gcatgcgcgc gatgtggatc gcctcggggt cggccacttc catgcattcg
      601 gcgaggtagc cttccgccgg cagcatcagc gcttccgccg cgaacgcggg gtccttggag
      661 ccgtcactga tcacgcggcc catggcgtcg atgaaggtcg gcggcacttc catggcgcgg
      721 ccctcgcgga cgctgtcgat cgaggcgagc aggatgcgga tcgcgagcgc ctgcccggcc
      781 tcccagcggt tgaaggcgtc ggagtcgtac gccatgaggt gcgcgagctc cgaatcggtg
      841 tagtcgtagc gcacgtgcac cggcgcggag aatccgcgca gcagcgaggg caccgggttc
      901 tccggtacct ggtggaagac gaacgattgt tccgccgccg tcagcgcgag cacagcggtg
      961 cgcgagccgt tggcgcgcgc gcccttgaag agcgatttcg agtcgatgtt gatcggcaag
     1021 tcgcggccgt ccaggtccac cagcccgacc gcgaggggaa tgacgaaggg cttcttcgcc
     1081 gcctggcccg gcgtgggcgg gcacgtctgc ttcgcgtgca gcgtgtaggt ctcgccccgg
     1141 gggtcgtatt cgccgcggca ttcgaccacc ggcgttcccg actgcgagta ccacaggccc
     1201 agctgcgcga ggtccacctg gttggcttcg gccatcgcag cgcggaagtc gtcgcaggtg
     1261 accgcctggc cgtcgtggcg cttgaagtag agatccatgc cgcggcggaa gccctccttg
     1321 ccgaggatgg tcgcgtacag gcgcacgacc tcggcgccct tgtcgtacac ggtggctgta
     1381 tagaaattgt tgatctcgac gtacgactcg gggcgcaccg ggtgcgccat cggacccgcg
     1441 tcctcgggga actggcggtt gcgcaggtcg cgtacttcct ggatgcgcac cacggggcgc
     1501 gagtattcgt ccgcgctgta ttcctggtcg cggtagaccg tgaggccttc cttgagcgag
     1561 agctggaacc agtcgcggca cgtcacccgg ttgccggtcc agttgtggaa gtactcgtgc
     1621 gcgaccaccc tgtcgatcag catgtagtcc gtatcggtcg cggtgtcggg gcgcgccagc
     1681 acgaagctgg tgtt
//
LOCUS       DASVII010000424         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_702610,
            whole genome shotgun sequence.
ACCESSION   DASVII010000424 DASVII010000000
VERSION     DASVII010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702610"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1782)
                     /locus_tag="VF309_05550"
     CDS             complement(34..1782)
                     /locus_tag="VF309_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7466086.1"
                     /translation="MAQPAPPRAGTSRKRTDDPALGKVIAGRYRLEARIGEGGMGIVY
                     RARHVLIDRVVAVKLIRPDLRGETHLRAWMLREARAANRVDHAHIIDIHDIGETDDSE
                     LYLVMEYLVGTPLSTELARGPLPLSRGVDILEQMGAALSRAHDLGVVHRDLKSDNILL
                     TARGGRKDFVKILDFGLAALAHDPRLAPKGAVFGTPEYMSPEQARGEQAGPQSDLYAL
                     GVLFFEMLTGQLPFRAGDRETLLEMQRTAPAPRPTTVKKDCHPVAERIVLRLLEKDPR
                     KRYRDGHHLLEELKALQRSLPSTSWDKEGGTGTSGEAPPAAQPPPPPPPKTPPVGEWA
                     TRAGLFARMVSRAYPSANAPAEVGQAVIAVWDVAAKANGLEGEVASHTRKLEALERRG
                     RALRAEIGRKVEELAHEESRSLREAAAYGEEEESARRELSRAQQIAEEKVGVADRADR
                     AGQGTRPIFEAAGAAAAMVEAQRQWLKTRETRRMAREASAADLRRQIEELRGQLARYA
                     EALEEDLASGREKVAARAREGLKFEKIFLDASAVLIGHLKGRPECRDLMNEIMAPAPE
                     EGPSTSTPGARVDGQS"
BASE COUNT          300 a          661 c          687 g          376 t
ORIGIN      
        1 caaccgggta aaaccaaacg agatcacccc gggctagctt tgcccgtcga cccgtgcccc
       61 gggcgtcgag gtacttggac cctcctccgg agcaggagcc atgatctcgt tcatgagatc
      121 gcgacattct ggccggccct tgaggtgacc gatgagcacc gcggaggcgt cgaggaagat
      181 cttctcgaat ttgaggcctt cacgggcgcg ggcggcgact ttttcgcgtc cggatgccag
      241 atcttcttcg agcgcttcgg cgtagcgcgc gagctgcccc cgcagctctt cgatttgtcg
      301 acgcagatcg gcggcgctgg cttcccgcgc catgcgccgt gtttcgcgcg tcttgagcca
      361 ctgacgctga gcctcgacca tggcggcggc ggcgcctgcg gcttcgaaaa tcgggcgcgt
      421 gccctgtccg gcacgatcgg cacggtcggc cacgcccact ttttcttcgg cgatttgttg
      481 cgcgcgtgat agctcgcgcc gcgccgactc ttcttcttcg ccgtaggcgg cggcttcgcg
      541 cagcgagcgc gactcttcgt gtgccagctc ttccaccttg cggccgattt cggcgcgcag
      601 cgcgcgaccg cgccgttcga gcgcctcgag cttgcgcgtg tggcttgcga cctcgccttc
      661 gagaccgttg gctttggccg cgacgtccca caccgcgatc actgcctgcc caacctctgc
      721 gggcgcgttg gccgaggggt aggcgcgcga caccatacgg gcgaagagcc cggcgcgcgt
      781 ggcccactcg cccaccgggg gcgtcttggg cggcgggggt ggcggcggct gcgcggcggg
      841 gggcgcttcg cccgaggtac cggttccgcc ttctttgtcc cacgaagtgc tgggtaacga
      901 acgctgcagg gccttcagct cttcgagcag atgatggccg tcgcggtagc gcttgcgcgg
      961 atctttctcg agcagcctga gcacgatgcg ctcggccacc ggatggcagt ccttcttgac
     1021 ggtcgtcggg cgcggcgccg gggcggtgcg ctgcatttcg agcaatgttt cgcgatcacc
     1081 ggctcggaac ggtaactgtc cggtgagcat ttcgaagaac aacacgccga gcgcgtacaa
     1141 gtccgattgg ggacccgcct gctcgccgcg ggcctgctcc ggcgacatgt actcgggcgt
     1201 accgaagacg gcgcctttcg gcgccagccg tggatcgtgc gccagggcgg ccagaccgaa
     1261 gtcgaggatt ttgacgaagt ctttgcggcc accgcgcgcg gtgagcaaaa tgttgtcgct
     1321 cttcaagtcg cgatgcacga caccgagatc gtgcgcgcgc gacaaggcgg cgcccatttg
     1381 ctccaagatg tcgacgccgc gactgagcgg caaaggtccg cgcgccagct ctgtggagag
     1441 cggcgtgccg accaggtact ccatgacgag gtacagctcg ctgtcgtcgg tctcgccgat
     1501 gtcgtggatg tcgatgatgt gcgcgtggtc gacgcgattg gcggcgcgcg cctcgcgcaa
     1561 catccatgcg cgcaggtgcg tttcgccgcg taagtcaggt cggatcagct tgacggccac
     1621 gacacggtcg atgagcacgt gccgggcgcg atagacgatg cccatcccgc cctcgccgat
     1681 gcgcgcctcg agccgatacc ggccggcgat gaccttgcca agcgcgggat cgtccgtccg
     1741 cttacgagat gtcccagccc gtgggggcgc tggttgtgcc atgtttccgg tccaaacctg
     1801 gggtgaggaa gttgcctgac ccgcaagctt agccacaaca cccccggatt accaccgggt
     1861 gtcgctcaga acgctacgtc tagaactggg attcgaactt cggtggcgtc gggcgcgttg
     1921 aacatcatcc gctcggcgcg gctgtaggcg tgcagcacgg cgatgtttgg aacgccgagt
     1981 gcaagtcgcg cgtctttcac gccgaacagc gccggtggtg ggag
//
LOCUS       DASVII010000425         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_702636,
            whole genome shotgun sequence.
ACCESSION   DASVII010000425 DASVII010000000
VERSION     DASVII010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702636"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /gene="obgE"
                     /locus_tag="VF309_05555"
     CDS             complement(<1..633)
                     /gene="obgE"
                     /locus_tag="VF309_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX7466087.1"
                     /translation="MKFFDEAKIEVVAGKGGDGSASFRREKFVPRGGPDGGDGGRGGS
                     IFAVADSNLNTLIDFRYTRIFRAKGGENGRGRDQYGKGAEDIVLRMPVGTVIKDAESG
                     TVIADLAADGDKALLVKGGKGGIGNLHFKSSVNRAPRQFTRGEEGEQRKLEMELRVLA
                     DVGLLGMPNAGKSTFIRAVSAARPKVADYPFTTLHPNLGVVRVAEDKSFVV"
     gene            complement(630..>1625)
                     /locus_tag="VF309_05560"
     CDS             complement(630..>1625)
                     /locus_tag="VF309_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX7466088.1"
                     /translation="NLEALWIARELRPGTKVVASQQAHYTHRRLSAVLGVDFEAVACD
                     GAGRMDMAALERILAAGNVGTVVATLGTTAIGSVDPLPRILELRERHGFRLHADAAYG
                     GYFVLAANLAENARLAFERIAEADSIVVDPHKHGLQPYGCGCVLFKDPSVGRFYKHDS
                     PYTYFSSSELHLGEISLECSRPGASAVGLWATQRLLPLEKGGEFASMLERSRAAALAL
                     FERIRADARFMTAFAPELDIVVWAPRAASAGEASALSRRIFDEAARRNLHLALADLPM
                     EFFAANAGAMRRDRPTVTCLRSVLMKPEHLDWVERIWAIVDAATQAASREAGAHP"
BASE COUNT          244 a          563 c          570 g          248 t
ORIGIN      
        1 gacgacaaag ctcttgtcct cggccacgcg caccaccccg aggttgggat gcagcgtggt
       61 gaacggatag tcggcgacct tcgggcgcgc ggccgacacg gcgcggatga aagtcgattt
      121 cccggcgttg ggcatgccga gcagacccac gtcggccagc acgcgcagct ccatctcgag
      181 cttgcgttgc tcgccttcct cgccgcgcgt gaactggcgc ggcgcgcgat tcacgctcga
      241 cttgaagtgg aggttgccta tgccgccctt gccgcccttg acgaggagcg ccttgtcgcc
      301 gtccgcggcg aggtcggcga tgacggtgcc gctttcggca tccttgatga cggtgccgac
      361 gggcatgcgc agcacgatgt cctcggcgcc cttgccgtac tggtcgcgcc cgcggccgtt
      421 ctccccgccc ttcgcgcgga agatgcgcgt gtagcggaaa tcgatgaggg tgttgaggtt
      481 ggagtccgcg accgcgaaga tgctgccgcc tcggccgccg tcgccgccgt cggggccgcc
      541 gcgaggcacg aacttctcgc ggcggaagga cgccgagccg tccccgccct tgcccgcgac
      601 gacctcgatc ttcgcttcgt cgaagaattt catggatgcg cgcccgcttc gcgcgacgcc
      661 gcttgtgtcg ccgcgtccac gatcgcccag atgcgctcga cccaatcgag gtgctcgggc
      721 ttcatgagca ccgatcgaag gcacgtcacc gtcggccggt cgcgcctcat cgcgccggcg
      781 ttggcggcga agaattccat cggcaggtcg gcgagggcga ggtggagatt ccttcgcgcg
      841 gcctcgtcga agatgcggcg cgaaagcgcc gacgcttcac cggcgctcgc ggcgcgcggc
      901 gcccagacga cgatgtcgag ctcgggcgcg aacgccgtca tgaagcgcgc gtccgcgcgg
      961 atcctctcga agagggccag cgcggcggcg cgcgagcgct cgagcatcga cgcgaactcg
     1021 ccccctttct cgagcggcag cagccgctgc gtggcccaga gcccgaccgc ggaggcgccc
     1081 gggcgcgagc actccaggct gatctcgccg agatgcagct cgctcgagct gaagtaggtg
     1141 tacggcgagt cgtgcttgta gaagcgtccc accgacggat ccttgaagag cacgcagccg
     1201 catccgtagg gctgcagccc gtgcttgtgc ggatccacca cgatcgaatc ggcctcggcg
     1261 atgcgctcga acgcgagacg ggcgttctcc gcgagattcg cggcgaggac gaagtagccc
     1321 ccgtaagcgg cgtcggcgtg cagccggaac ccgtggcgct cgcgaagctc gaggatgcgc
     1381 gggagcggat ccaccgagcc gatggcggtc gtgccgagcg tcgcgacgac ggtgcccacg
     1441 ttgcccgcgg cgagaatccg ctcgagcgcg gccatgtcca tgcgcccggc gccgtcgcag
     1501 gcgacggctt cgaaatcgac gcccagaacg gcgctcaggc gccgatgcgt gtagtgcgcc
     1561 tgctgcgagg cgacgacctt cgtgccggga cgcagctcgc gcgcgatcca caacgcctcg
     1621 aggtt
//
LOCUS       DASVII010000426         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_594759,
            whole genome shotgun sequence.
ACCESSION   DASVII010000426 DASVII010000000
VERSION     DASVII010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594759"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1325)
                     /locus_tag="VF309_05565"
     CDS             complement(123..1325)
                     /locus_tag="VF309_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7466089.1"
                     /translation="MNSTDKRSAWIVTLAAAAILMVTMGARQSLGLFVSPLNTSTGLG
                     VVTISLALAVAQFAWGAVQPVAGAVADRYGPGKVLVAGLVVLSIGTALTPFMTSGLGL
                     IVSLGLLSAIGSGAGSFSVLIGAAAKRLPPESRGNAAGVINAGGSFGQFVFAPLCQRL
                     IQGLGWMGAMWTLAVITLAALPLVRVVTPRDPPKPHPAGEAGMWPTVRGALGDRSYIL
                     LHLGFFTCGFHIAFLVTHLPQEVNLCGLPPTVASWSLAIIGLANIAGSLMAGASTQRY
                     RSKYILALMYGSRAALIGWYLAAPKTQLTFYFFAAGLGLTWLATVPPTAAIVGKLFGV
                     RYLATLFGLTLLSHQIGGFLGAYLGGIALDRNGNYQWMWYADMTLAAMAAVVNLPIRE
                     PEMRAAPA"
     gene            complement(1404..>2191)
                     /locus_tag="VF309_05570"
     CDS             complement(1404..>2191)
                     /locus_tag="VF309_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466090.1"
                     /translation="AVVGEQGLGDVVFFLRYAPLLRARGARLDFFGEPRLHGMLARTG
                     LFEKLAARSDSPRADGAIEILAGDLPLLAPEAQSPASVPAPLPLRADPARVAAIRERL
                     HALGPPPHIGIAWRSGEPRVGLFETLFKEVPLDGLGLALRERRATLVSVQRAPGPVET
                     ESVASHAQRPVHDLSALNADLEDALALMSVLDDYVGVSSTNVHLRAGAGRASRVLVPF
                     PYEWRWMREGASPWFPGTTVLRQSATGSWSDAFTQLMQGADIQ"
BASE COUNT          388 a          724 c          791 g          288 t
ORIGIN      
        1 gtgtgatcgc tgagcatcaa gccctgggca cgaaaaagca ccagtgaatc cacggtggct
       61 catgtccgct atgggtcgga agccgacctt caaactgaga tactaaagtt tccggccgga
      121 agtcaggccg gtgcggcccg catctccggc tcgcggatcg gcaggttgac gacggcggcc
      181 atggcggcga gcgtcatgtc cgcgtaccac atccattggt agttgccgtt gcggtcgagg
      241 gcgatgccgc cgagatacgc gccgaggaat ccgccgatct ggtgcgacag cagggtcagg
      301 ccgaagagcg tcgccaggta gcgcacgccg aagagcttgc ccacgatcgc cgcggtcggc
      361 gggacggtcg cgagccacgt gaggcccagg ccggcggcga agaagtagaa cgtcagctgc
      421 gtcttgggcg cggcgagata ccacccgatg agcgccgcgc gcgacccgta catgagcgcg
      481 aggatgtact tgctgcgata gcgttgcgtc gaggcgcccg ccatgaggct ccccgcgatg
      541 ttggcgaggc cgatgatcgc gagcgaccag ctcgcgacgg tgggcggcaa tccgcacagg
      601 ttcacctcct gcggcaggtg cgtcacgagg aacgcgatgt ggaagccgca cgtgaagaac
      661 ccgaggtgca gcaggatgta gctcctgtcg cccagggcgc cgcgcaccgt gggccacatt
      721 cccgcttcgc ccgcgggatg gggcttcggc ggatcccgcg gcgtgacgac gcgcaccagc
      781 ggcaacgcgg cgagcgtgat cacggcgagc gtccacatcg cgcccatcca tcccagcccc
      841 tggatgagcc tctggcagag cggcgcgaac acgaactgcc cgaaggatcc tcccgcgttg
      901 atcacgccgg ccgcgttgcc gcgcgattcg ggaggcaggc gcttggccgc cgcgccgatc
      961 agcaccgaga agctgccggc gcccgagccg atcgccgata ggaggcccag cgagacgatg
     1021 aggccgaggc cggaggtcat gaagggcgtg agcgcggtgc cgatggagag cacgacgaga
     1081 ccggcgacga gcaccttgcc gggcccgtag cgatccgcga ccgcgcccgc gaccggctgc
     1141 accgcgcccc acgcgaactg cgcgacggcg agggcgaggc tgatggtcac gacgcccagc
     1201 cccgtcgagg tgttgagcgg cgagacgaag aggccgagcg attgccgggc gcccatcgtc
     1261 accatgagga tcgccgccgc cgcgagcgtg acgatccatg cgctgcgctt gtccgtcgag
     1321 ttcatggccg acatgatgcc atcaatcccg cggcgcgcgt cacctcatgc atgagttata
     1381 cgcatgcgtg gacttgcatg cgactactga atgtcagcac cttgcatgag ctgggtgaat
     1441 gcatcgctcc agctgccggt cgccgactgg cgaaggaccg tggtgccggg aaaccacggc
     1501 gacgctcctt cgcgcatcca gcgccactcg tagggaaagg gcacgaggac gcgcgaggcg
     1561 cgcccggccc cggcgcgaag gtgcacgttg gtgctgctga cgcccacata gtcgtcgagc
     1621 acgctcatca gcgcgagcgc atcctcgaga tcggcgttca gggcggaaag atcgtggacg
     1681 gggcgctggg cgtgcgacgc gacgctttcc gtttccacgg gtccgggagc gcgctgcacg
     1741 gagaccagcg tagcccgcct ctcgcgcagc gcgaggccga gcccatcgag cggcacctcc
     1801 ttgaagagag tctcgaatag ccccacgcgg ggctctcccg agcgccatgc gatgccgatg
     1861 tgaggtgggg ggccgagggc gtgcagccgc tcgcgaatcg ccgcgacccg cgcgggatcg
     1921 gcgcgcagag gcagcggcgc gggcaccgag gccggcgatt gcgcttcggg cgcgagcagc
     1981 ggcaaatcgc ccgcgaggat ctcgatcgca ccgtccgcgc gaggcgaatc ggagcgtgcc
     2041 gcgagcttct cgaaaagccc cgtgcgcgcg agcattccgt ggagccgcgg ctcgccgaag
     2101 aaatcgaggc gggcgccgcg cgcgcgaaga agcggcgcgt agcgcaggaa gaagaccacg
     2161 tcgccgaggc cctgctcgcc gacgacggcg a
//
LOCUS       DASVII010000427         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_127474,
            whole genome shotgun sequence.
ACCESSION   DASVII010000427 DASVII010000000
VERSION     DASVII010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127474"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VF309_05575"
     CDS             complement(<1..461)
                     /locus_tag="VF309_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7466091.1"
                     /translation="MGLGYVGLPLAVQMGRAGLKVIGFEVSERVAKLVNAGESHIKDV
                     NGSDVAALRRAGKLEATTDMSRLGECDVISLCVPTPLGKTKDPDMSYIVAATEAIAER
                     LRPGQLVVLESTTYPGTTRELMLPRFESNGLTVGKDFFLCFSPERVDPGNPV"
     gene            722..>1799
                     /locus_tag="VF309_05580"
     CDS             722..>1799
                     /locus_tag="VF309_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7466092.1"
                     /translation="MPCSHNGCPLGVDAPAIASALLAGDHPRAFLLARGPNPLASVCG
                     HGCHAPCETACNRRAFGAPVAIAALEAYASGFSTPQTLPSPEPCTTAHDARSVAGLVG
                     QTPDTAFRAPRSGKRVAIIGAGAAGMGCAHDLSLLGHESTIFDESSEPGGVLTAAIPS
                     FRFPVASARAECTAILSTHAQLASNAPVTGIETLRAMLATEFDAIFLAIGASIPANGM
                     FPEQPPHPRVVDAMSVLAKHVPLSGRVVVIGDGDLALDAARVALRRPRSEEARSVATA
                     HAVLPEPLEQSSAQPAALAAALQDGVILHGGWRATRYLTDTEGTLTGVEIAHAIEGVT
                     KVLACDSLVTAARRIPARQFGAELK"
BASE COUNT          279 a          661 c          603 g          256 t
ORIGIN      
        1 acgggattgc cgggatcgac gcgctcgggc gagaagcaga ggaagaagtc cttcccaacc
       61 gtcagcccat tcgactcgaa gcgcggcagc atcagctcgc gcgtcgtgcc gggatacgtc
      121 gttgactcga gcaccacgag ctgccccgga cgaagccgtt ccgcgatcgc ctcggtcgcg
      181 gcgacaatgt acgacatgtc gggatccttc gtcttgccga gcggtgtggg cacgcaaagg
      241 gagatgacgt cgcactcgcc caggcgcgac atgtccgtcg tcgcctcgag cttgcccgcg
      301 cggcgcagcg ctgccacgtc ggagccgttc acgtccttga tgtggctctc gccggcgttg
      361 acgagcttcg ccacccgttc cgagacctcg aaaccgatca ccttgagccc ggcgcgtccc
      421 atctggacgg ccaagggcag cccgacgtag ccgaggccca tgacaccgca caccagctcg
      481 cggcggtcga ttttgtccga cagcgattcg aaagttccca caggccgatg attgggtgga
      541 gaggaagcga cgctgggagg acgccccccg cgcaacgcgg taacctcgcc ggggcgtcgg
      601 cagtctgaaa gaagagcgcg gcgcgcgcac gcctcagatg tgggaactta acggaagcaa
      661 attgaccgat caagcaaaac gctaccccct gccgctggcg ccaaccgcgc tctcggccct
      721 cgtgccgtgt tcccacaacg gctgcccgct cggcgtcgac gccccggcga tcgcgagcgc
      781 gctcctggcg ggcgaccacc cgcgagcttt cctgctcgcg cgcggtccca acccgctcgc
      841 gagtgtctgc gggcacggct gccacgcgcc ctgcgagacc gcctgcaacc gccgcgcgtt
      901 cggcgcgccg gtcgcgatag cggcgctcga ggcgtacgcg agcggcttct cgacgccgca
      961 gacgctcccc tcgcccgagc cctgcaccac cgcgcacgac gcgcgctccg ttgccggcct
     1021 cgtgggccag acgccggaca ccgcattccg cgcgccgcgt tccgggaaac gcgtggccat
     1081 catcggcgcc ggcgccgccg gcatgggatg cgcgcacgat ctctccctgc tcggccacga
     1141 gagcacgatc ttcgacgagt cgagcgagcc gggcggcgtg ctcacagctg cgatcccgtc
     1201 gttccgcttt cccgtagcga gtgcgcgcgc cgagtgcacg gcgattctgt cgacgcacgc
     1261 acagctcgca tcgaacgcgc cggtgacggg gatcgagact ctgcgtgcga tgctcgcgac
     1321 ggagttcgac gcgatcttcc tcgcgatcgg cgcgtcgata ccggcgaacg gaatgtttcc
     1381 ggagcagccg ccgcatccgc gcgtcgtcga cgcgatgtcg gtgctcgcga aacacgtgcc
     1441 gctgagcggg cgcgtcgtcg tgatcggcga cggcgatctc gcgctcgacg ccgcgcgcgt
     1501 ggcgcttcgg cgcccgcgca gcgaagaggc gcggagcgtc gcgaccgcgc acgcggtgct
     1561 ccctgagccg ctcgagcaga gctccgcaca gcccgccgca ctcgcggccg cgctgcagga
     1621 tggcgtgatc ctgcacggcg ggtggcgcgc gacgcgctac ctcacggaca ccgaaggcac
     1681 gctgaccggc gtcgagatcg cgcatgccat cgagggcgtc accaaggtgc tcgcgtgcga
     1741 ctcactcgtc accgccgcac ggcgcatccc cgcgcgccag ttcggcgcgg agctcaagc
//
LOCUS       DASVII010000428         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_20049, whole
            genome shotgun sequence.
ACCESSION   DASVII010000428 DASVII010000000
VERSION     DASVII010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20049"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VF309_05585"
     CDS             <1..254
                     /locus_tag="VF309_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466093.1"
                     /translation="ELYAEKAINTFETIGPAQAKRFSDETSFDPVAENWLGAPMDFQN
                     EVLANELRMALMAGAGIGESDLVPNPMPALRNVEMFNVA"
     gene            256..1860
                     /locus_tag="VF309_05590"
     CDS             256..1860
                     /locus_tag="VF309_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466094.1"
                     /translation="MTNALLDVDRVIGLKTVREATSRGQFLSAIRELGKDSGEFTLAR
                     LHELMRVEEHFQALLLYDQIDFLRQPASASDRDRDFALNIQRICLEAANGFQRFLRNR
                     NAWATTREALDTMFRVTGLALNSIHSFVKWGYFLNEPGRATPWKQVHALYALAEADGY
                     SQVPFVLHAPQPSYKPSVQSLYLRTLILDLLNTGNLSNIQIEIADGWFSSWCNDYSLD
                     TEYSAGNHLFFVDLASDTGMRLVRKDSQGDSMRYVRTEGLKTQIEEVQLGLRHGRLYA
                     GYGAGAVFPVEEHVALLAIIEKLYHSILAGSENRIEERTHFEDREVDVVIGIQRVMRK
                     MREAPSKATSASRQAAPSAPVAETFEISPTGMSLVANEEAAAAPFEEPADPDIERWRV
                     YDLSSKGYGLLVDRAASELVLLNGIIALRNHETGGWIVGLVVRKLANRVRGEILIGVE
                     VLAYRPLPADLVPASGGPPVQALYLPGLDTNGKFDSVIVGVGEFRTDQTYGIEAGGSK
                     YRVRLNRIIRKGADWLKARFEIESKA"
     gene            complement(1826..>2044)
                     /locus_tag="VF309_05595"
     CDS             complement(1826..>2044)
                     /locus_tag="VF309_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEX7466095.1"
                     /translation="GDAIVAAKLGERFPGIDAGALEVGIWSRVASAATVLREGDRVEL
                     YRALKADPKDARRARARLRPSTRSRSGP"
BASE COUNT          356 a          698 c          660 g          330 t
ORIGIN      
        1 acgagctcta tgccgagaag gcgatcaata cgttcgagac catcgggcct gcgcaggcga
       61 agcgcttctc ggacgagacg agcttcgatc cggtcgcgga gaactggctc ggcgctccga
      121 tggacttcca gaacgaggtg ctcgccaacg agctgcgcat ggcgctgatg gccggagcgg
      181 gcatcggcga aagcgatctc gtgccgaacc cgatgccggc gcttcgcaac gtcgagatgt
      241 tcaacgtcgc ctgacatgac caacgccctc ctcgacgtcg atcgcgtcat cgggctgaag
      301 accgtccgcg aggcgacgtc gcgggggcag ttcctctcgg ccatccggga gctcggcaag
      361 gacagcggcg aattcacgct cgcgcggctt cacgagctga tgcgcgtcga ggagcatttc
      421 caggcgctgc tcctctacga ccagatcgat ttcctgcgcc agcccgcgtc ggcttccgac
      481 agagaccgcg atttcgcgct caacatccag cgcatctgcc tcgaggccgc caacggtttc
      541 cagcggttcc tgcgcaaccg caatgcgtgg gcgacgacac gcgaagcgct cgacacgatg
      601 tttcgcgtga cggggctcgc gctgaactcg atccacagct tcgtgaagtg gggatacttc
      661 ctcaacgagc ccggccgcgc gacgccgtgg aagcaggtgc atgcgctcta cgccctcgcc
      721 gaagccgacg ggtattcgca ggtccctttc gtgctgcatg ccccgcagcc gagctacaag
      781 ccctcggtgc agtcgctcta cctgcgcacg ctgatcctcg atctgctcaa caccggcaac
      841 ctcagcaaca tccagatcga gatcgccgac ggctggttct cctcgtggtg caacgactat
      901 tcgctcgaca ccgagtactc ggcgggcaac cacctcttct tcgtagacct cgcctcggac
      961 acgggcatgc gcctcgtgcg caaggacagc caaggggatt cgatgcgcta cgtgcgcacc
     1021 gaaggcctca agacccagat cgaggaagtg cagctgggat tgaggcacgg gcgtctctac
     1081 gcgggctacg gcgcgggcgc ggtattcccg gtcgaggagc acgtggcgtt gctcgcgatc
     1141 atcgaaaagc tctaccactc gatcctcgcc ggcagcgaga accgcatcga ggagcgcacg
     1201 cacttcgagg accgcgaggt cgacgtcgtg atcggcatcc agcgcgtgat gcgcaagatg
     1261 cgcgaggcgc cgtcgaaggc gacgagcgct tcgcggcagg ccgcgccttc cgcgcccgtc
     1321 gcggagacgt tcgagatttc tccgacggga atgtcgctcg tcgccaatga agaggccgcg
     1381 gcggcgccct tcgaggagcc cgccgatccg gacatcgaac gctggcgcgt ctacgacctc
     1441 agctcgaagg gctatgggct gctggtcgat cgggccgcga gcgagctcgt gctgctgaac
     1501 ggcatcatcg cgctacgcaa tcacgagacg ggcggatgga tcgtgggcct ggtcgtgcgc
     1561 aagctcgcga atcgcgtgcg cggcgagatc ctcatcgggg tcgaagtgct ggcctaccgg
     1621 cccttgccgg ccgacctcgt gcccgcctct ggcggcccac ccgtgcaggc gctctacctg
     1681 ccggggctcg acaccaacgg caagttcgat tccgtcatcg tcggcgtggg cgaattccgc
     1741 accgaccaga cctacgggat cgaggcgggt ggctcgaaat accgcgtgcg gctgaaccgc
     1801 atcattcgca agggcgcgga ctggctcaag gcccgcttcg agatcgagtc gaaggcctga
     1861 gccgcgcccg ggcgcggcgc gcgtccttgg gatcggcctt cagcgcgcgg tagagctcca
     1921 cgcggtcgcc ttcacgaagc accgtcgcgg cgctcgccac ccgcgaccaa attcccacct
     1981 cgagggcgcc cgcgtcgatc cccggaaagc gctcgcccag cttcgctgca acgatcgcgt
     2041 cgcc
//
LOCUS       DASVII010000429         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_594851,
            whole genome shotgun sequence.
ACCESSION   DASVII010000429 DASVII010000000
VERSION     DASVII010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594851"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..175
                     /locus_tag="VF309_05600"
     CDS             101..175
                     /locus_tag="VF309_05600"
                     /inference="COORDINATES: protein motif:HMM:NF022060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7466096.1"
                     /translation="MSRLHDRIAYRRLGNSWEITRLAP"
     gene            172..804
                     /locus_tag="VF309_05605"
     CDS             172..804
                     /locus_tag="VF309_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX7466097.1"
                     /translation="MSAVRHAPPASAAKPLKPTELLASLRNALEPWEPGTPFWIFAYG
                     SLMWNPKFTWDARHVATIRGYHRSFRLWSRINRGTPDNPGLVLTLECGGSCRGLVYRI
                     APDLVQEQMQLIWKREMMFGSYRPRWLNCVAGGETIRALCFTVNRDCSGYTGEIPIEV
                     MVESLASAAGRYGPAHDYLFKTTETLHEHGIRDARVEHLAQLVKARIKTA"
     gene            complement(820..1680)
                     /locus_tag="VF309_05610"
     CDS             complement(820..1680)
                     /locus_tag="VF309_05610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7466098.1"
                     /translation="MSIARVGVVGVGAMGMGVARALIAKGFTVCVRDVVAAREEQAIA
                     AGAERISGDVDVLVSLVVDAGETRAVVEEHGGRAPAFMMCSTIAPRDAERFASALQAC
                     GVEMLDAPISGGPARAHAGTLSMMAAGSEAAFERCKPVIGAITAKCFRMGAKPGDGSR
                     MKVVNNMMAASNIAAACEAMAMASKLGIDLERAMAVVNASSGASWMFADRMPRALARD
                     YAPTAAARVLLKDVGLFVEIARSFGLSAPMAEQAQAIFRDTCERGFAEEDDAAVLKRY
                     ADEWKVDVPR"
     gene            complement(1677..>2025)
                     /locus_tag="VF309_05615"
     CDS             complement(1677..>2025)
                     /locus_tag="VF309_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7466099.1"
                     /translation="SFERTTLAALGMPPQMAMRHRLPHFSHLFSSDSYAAGYYSYLWA
                     DTLSADAFEAFTEAGGAYDKSVARKLRDHVFSAGNTVDPAEGYRAFRGRDAQIDALMR
                     RRGFGEGHASRIR"
BASE COUNT          309 a          736 c          678 g          302 t
ORIGIN      
        1 gaaggagctc ggggcgcgcc atggcgagga tcccgagcgt ccgccgcatt ggggcggcta
       61 ccgcctcgtt cccgagcaag tcgagttctg gcagggacgc ctgagccgcc tgcacgatcg
      121 catcgcgtac cgccgcttgg gcaactcgtg ggagatcacg aggctcgccc cgtgagcgcc
      181 gtgcgccacg cgccgcccgc ctccgcggcg aaaccgctca agcccacgga gctcctcgcg
      241 tcgcttcgca acgcgctcga accgtgggag ccgggaacgc ccttctggat cttcgcctac
      301 ggctcgctca tgtggaaccc gaagttcacc tgggatgcga ggcacgtcgc gaccatccgc
      361 ggctaccacc gctccttccg cctgtggtcg cgcatcaatc gcggcacgcc cgacaatccc
      421 gggctcgtcc tcacgctcga gtgcggcgga agctgccgcg gcctcgtcta tcgcatcgcc
      481 ccggacctcg tgcaggagca gatgcagctc atctggaagc gcgagatgat gttcggctcg
      541 taccgcccga ggtggctgaa ctgcgtcgcg ggcggcgaga ccattcgcgc cctgtgcttc
      601 accgtgaacc gcgattgctc cgggtacacg ggcgagatcc ccatcgaggt catggtcgag
      661 tcgctcgcga gcgcggcggg gcgctacggg ccggcgcacg actatctctt caagacgacc
      721 gagacgctgc acgagcacgg catccgcgat gcgcgcgtcg agcacctcgc ccagctcgtg
      781 aaggcgcgca tcaagacggc ttgaagcggc ggctcgtctt catcggggaa cgtcgacctt
      841 ccactcgtcc gcgtagcgct tcaggaccgc ggcgtcgtct tcctcggcga agccgcgctc
      901 gcacgtgtcg cgaaagatcg cctgcgcctg ctccgccatg ggcgcggaaa ggccgaacga
      961 gcgggcgatc tcgacgaaga ggccaacgtc cttcagcagc acgcgcgccg cggcggtggg
     1021 cgcgtagtcg cgggcgagag cgcgcggcat gcggtcggcg aacatccagc tcgcacccga
     1081 gctcgcgttc accaccgcca tcgcgcgctc caggtcgatg ccgagcttcg acgccatcgc
     1141 catcgcctcg cacgcggcgg cgatgttgga cgcggccatc atgttgttca ccaccttcat
     1201 gcgcgatccg tcgccgggct tcgctcccat gcggaagcac ttggcggtga tcgcgccgat
     1261 caccggctta cagcgctcga acgccgcctc gcttcccgcg gccatcatgc tgagcgttcc
     1321 cgcgtgggcg cgcgccgggc cgcccgaaat cggcgcgtcg agcatctcga ccccgcaggc
     1381 ttgcagcgcc gaagcgaacc tctccgcgtc gcgcggcgcg atggtgctgc acatcatgaa
     1441 ggccggcgcg cggccgccgt gctcctcgac gacggcgcga gtctcccccg catcgacgac
     1501 cagcgacacg aggacatcga catcgccgga aatgcgctcg gccccggccg cgatcgcttg
     1561 ctcctcgcgc gcggcgacta cgtcgcgcac gcagaccgtg aagcccttcg cgatgagcgc
     1621 cctggcgaca cccatgccca tcgcgccgac tccgaccaca ccgacgcgcg cgatgctcac
     1681 cggatgcgcg aagcgtggcc ttcgccgaag ccgcggcggc gcatcagcgc gtctatttgc
     1741 gcgtcgcgcc cgcgaaacgc gcggtagccc tcggcaggat cgaccgtgtt gccggcggag
     1801 aagacgtggt cgcgaagctt cctcgcgacc gacttgtcgt aggcgccgcc cgcttcggtg
     1861 aacgcctcga aggcgtcggc ggagagcgtg tcggcccaca ggtagctgta gtagccggcc
     1921 gcgtacgagt cgctgctgaa gaggtggctg aagtgcggca ggcggtggcg catcgccatc
     1981 tgcggcggca tgccgagcgc ggcgagcgtc gtgcgctcga acgac
//
LOCUS       DASVII010000430         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_307140,
            whole genome shotgun sequence.
ACCESSION   DASVII010000430 DASVII010000000
VERSION     DASVII010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307140"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..311
                     /locus_tag="VF309_05620"
     tRNA            238..311
                     /locus_tag="VF309_05620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:270..272,aa:Gly,seq:ccc)
     gene            367..1446
                     /gene="mutY"
                     /locus_tag="VF309_05625"
     CDS             367..1446
                     /gene="mutY"
                     /locus_tag="VF309_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7466100.1"
                     /translation="MARPSSARKPRAPGAFPSRVVAWQRLHGRHDLPWQATRDAYRIW
                     ISEIMLQQTQVATVIPYFERFLARFPDVAALAAASEDQVLALWSGLGYYARARNLHRA
                     AREVVARLGGMFPVRQAELVQLPGVGRSTAAAIAAFASGERCAILDGNVRRVLARHAG
                     IGGDPASAAVTARLWSEAEARLPARGIERYTQGMMDLGANVCLARAPLCLLCPVAADC
                     VARIEKRVDELPQRRVRGPVRRRRIAMLVVISRGEVLLEKRPAAGIWGGLWSLPEADA
                     EASPREALASEWGLEAARVDAIEPFEHAFTHFTLEVQPWRIELARASRVAQGKPATWV
                     ALDDLAGAALPSPIRRLLEKLVSET"
     gene            complement(1523..2140)
                     /locus_tag="VF309_05630"
     CDS             complement(1523..2140)
                     /locus_tag="VF309_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7466101.1"
                     /translation="MVLFNIFSAGRKRETLPIFPLGTVLFPGAILPLRIFEVRYMDMA
                     KACLREGTPFGVCLIREGSEVGAPAVPEPVGCTARIAECDVEELGILKVRAEGIDRFR
                     IVSREVNAQGLIVGEVEPLAAEAQVDGIDGLAESAEFLRKVILAIGAGRFNEPYRFDD
                     ATWVSFRLAEILPLRTDVKQKLLELTDATLRLAVLHRFLRQQNLL"
     gene            complement(2169..>2618)
                     /locus_tag="VF309_05635"
     CDS             complement(2169..>2618)
                     /locus_tag="VF309_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX7466102.1"
                     /translation="QRIDTSMLTPSQLRGWVQDLVVTDRTRFTITFESFGFKGGVPLD
                     ADYVFDARCILPNPHYDPALRVQTGRDPDVVAFLERETQAGILIEDIQRFLQRWVPRF
                     LLDQRSSITVAIGCTGGRHRSVYVADQLAERFASEHHVVLRHRDLKG"
BASE COUNT          415 a          889 c          921 g          393 t
ORIGIN      
        1 tccagatgaa cgtgtagagg aaatcgcgtc cgaagctgca cggaaagcgc cggaagaaag
       61 ggatgatctc cagggggtgg aaatgctcgc ccccgaaggg cgccagcgcg gggagtctgc
      121 tcatggcgca atcctacctt cgccgcgccg gcgcacggga ccggcttgcg acggacggct
      181 gccgcggggc gcgaggctcg cgggcggcgg cgcgaatggc gctagaatgc gaccgacgcg
      241 ggtgtagctc aatggtagag cagaagcttc ccaagcttac gacgagggtt cgattccctt
      301 cacccgctcc agcccctccg aaacgattgc gcctccggaa cgcctcccgc ggacaaccgc
      361 acgccaatgg cacgaccttc atccgcgaga aagccccgcg ctcccggcgc gtttccttcg
      421 cgcgtcgtcg cgtggcagcg gctccacggc cgccacgacc tgccctggca ggccacgcgc
      481 gacgcctatc gcatctggat ctccgagatc atgctgcagc agacgcaggt ggcgaccgtg
      541 atcccgtact tcgagcgctt cctcgcccgc ttcccggacg tggccgcgct tgccgccgcg
      601 tccgaagacc aagtgctcgc actctggagc ggcctgggct actacgcgcg cgcgcgcaac
      661 ctgcaccgcg ccgcgcggga agtggtcgcg cggctcgggg gcatgttccc ggtgcgccag
      721 gccgagctcg tgcagcttcc cggtgtaggc cgttcgaccg ccgcggcgat cgccgccttc
      781 gcgagcggcg agcgttgcgc catcctcgac gggaatgtgc gccgcgtgct cgcgcgccac
      841 gcggggatcg gcggcgatcc ggcgagcgcg gccgtcacgg cgcgtttgtg gagcgaggcc
      901 gaggcgcggc tgcccgcacg cggcatcgag cgctacacgc aaggcatgat ggacctgggg
      961 gcgaacgtgt gccttgcgcg cgcgccgcta tgcctcctgt gccccgtggc cgccgactgc
     1021 gtcgcgcgca tcgagaagcg cgtggacgag cttccgcagc gccgcgtgcg cgggcccgtc
     1081 cggcgcaggc gcatcgcgat gctcgtcgtg atttcccgcg gcgaggtcct gctggagaag
     1141 cgccccgccg ccgggatctg gggcgggctc tggtccctcc cggaggcgga tgcggaggct
     1201 tcgcctcgcg aggcgctcgc gagcgagtgg gggctggagg ccgcgcgcgt cgatgcgatc
     1261 gagcccttcg agcacgcctt cacccatttc acgctcgagg tgcagccgtg gcgcatcgag
     1321 ctcgcaaggg cgtcgcgagt tgcccaaggc aagcccgcga cctgggtcgc gctcgacgac
     1381 ctcgccggcg cggcgctgcc ttcgcccata cgcaggctcc ttgaaaaact ggtgtcggag
     1441 acttgaaatt ggtgtcaggt acgaatttcg gatgcatcgg cgcgccgatg cgcgctgccg
     1501 aaattcgtac ctgacaccaa tttcaaagca ggttctgctg gcgcaggaac ctgtgcagga
     1561 ccgcgaggcg cagcgtcgcg tcggtgagct cgagcagctt ctgcttcacg tcggtgcgaa
     1621 gcggcaggat ctcggcgagc cggaagctca cccaggtcgc gtcgtcgaag cggtagggct
     1681 cgttgaagcg cccggcgccg atcgcgagga tcaccttgcg aaggaactcc gcgctctcgg
     1741 cgagaccgtc gatgccgtcc acctgcgcct cggcggcgag gggctcgacc tctcccacga
     1801 tgaggccctg ggcgttcacc tcgcgcgaga cgatgcggaa gcgatcgatg ccctcggccc
     1861 tcaccttgag gatgccgagc tcctcgacgt cgcattcggc gatgcgcgcg gtgcagccga
     1921 cgggctccgg caccgcgggc gcgccgacct cgctgccctc gcggatcagg cacacgccga
     1981 acggagtgcc ttccctgagg caggccttcg ccatgtccat gtagcgcacc tcgaagatgc
     2041 gcaacggcag gatcgcgccc ggaaagagca ccgtgcccag cggaaagatg ggaagcgtct
     2101 cgcgcttgcg gcccgcggaa aagatgttga agaggaccaa ggttattcgc gcgcctggtg
     2161 cctcgcgcct agcctttcag gtcgcgatgg cgaaggacca cgtggtgctc ggaggcgaag
     2221 cgctcggcga gctggtcggc gacgtacacc gagcgatggc gccctccggt gcagccgatc
     2281 gccaccgtga tcgacgagcg ctggtcgagg aggaagcgcg gcacccagcg ctgcaggaag
     2341 cgctggatgt cctcgatgag gatccccgcc tgggtctcgc gctcgaggaa cgcgaccacg
     2401 tcgggatcgc ggcccgtctg cacgcgcagc gccggatcgt aatgcgggtt cggcaggatg
     2461 caacgcgcgt cgaagacata gtcggcgtcg agaggcacgc cgcccttgaa cccgaacgat
     2521 tcgaacgtga tcgtgaagcg cgtgcgatcg gtgaccacca aatcctgcac ccagccgcgc
     2581 agctgcgagg gcgtgagcat gctggtgtcg atgcgctg
//
LOCUS       DASVII010000431         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_702781,
            whole genome shotgun sequence.
ACCESSION   DASVII010000431 DASVII010000000
VERSION     DASVII010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702781"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..>2287)
                     /locus_tag="VF309_05640"
     CDS             complement(185..>2287)
                     /locus_tag="VF309_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7466103.1"
                     /translation="YELSRSDSVSDNSYLTPISPTSEAAEVIEAMAVPAGLRARRVML
                     DAGWWRQSGGAMIARVMDRRRVPRGPTPMTSPSLAEGTGWVALTPDLGGYRMRATDAE
                     TGKVVEWRVDESVAARLAPFAFTFYRRFDKRALGAGDMLRFAAAHGGQDLLLLLIAGF
                     AAGLVGLFTPIATGWLIDHAIPSRATQTVQSIIAGLALAGVTIVALDVLRSLAVLRFE
                     ARIGVAMQAALVDRVICAPARFFRDFSSGDLALRMGSVNTIQRTITGSTIGTFITSLF
                     LVSNVALMIAYSPSLTLAASGIVVLVIGLSTLIGLARLKVGPRIEAMDGKLSALQFEI
                     FAGIAKLRAGAAESRAFEQWYAKYHHFRNLNGVSARLTNWESVALNLLQPAATILVLF
                     LAWRLAPTSSMTTGDFVAFHAALFALLGAVYVLVSTALDLVNLKPVWDRARPILETPP
                     EDSGAAGERYDPLGAIALEKVSFAYPGGPAVLQDIDLAVRPGEFVAIVGASGSGKSTL
                     LRLLLGFESPSAGAVRFDAKDLATLDLRHLRRRLGTVLQNGKLWAGDILTNIVAAHPV
                     GVEAAWEAAKRAGLERDIEAMPMGLYTVVGEGLSTLSGGQRQRVLIARALVASPRILL
                     LDEATSALDNVSQAMVLRGLAALEATRIVIAHRLNTVRHADRIVVLDRGRIVQQGSYD
                     ELAGQSGPFRAMLARQVA"
BASE COUNT          381 a          783 c          814 g          309 t
ORIGIN      
        1 aagcgcgagc cgcagcccca gaaggtggtg aagatggaga ccatcgtcgt gagcgcgcag
       61 cgcgacaagc cggacctcgc gggcaccagg ctcgcccagg cggacagcaa gaccaagaaa
      121 gactagacct tccttcgcgg aaggctgaaa gggcctcggc gctgccgggg ctttcttttt
      181 ccggctaggc gacctggcgc gcgagcatcg cgcggaacgg cccgctctgg cccgccagct
      241 cgtcatatga gccctgctgc acgatgcgcc cgcgatcgag cacgacgatg cgatcggcgt
      301 ggcgcaccgt attgaggcga tgcgcgatga cgatgcgcgt cgcctcgagg gccgcgagtc
      361 cccgcagcac catcgcctgc gatacgttgt cgagcgcgct cgtcgcctcg tcgaggagca
      421 ggatgcgcgg cgacgcgacc agcgcgcggg cgatcagcac gcgttgccgc tgcccgcccg
      481 agagcgtgct caagccctcg cccacgaccg tgtagaggcc catcggcatc gcctcgatgt
      541 cgcgctcgag gcccgcgcgc ttcgccgcct cccacgccgc ctcgacgccg acgggatgcg
      601 cggcgacgat gttggtgagg atgtcgccgg cccagagctt gccgttctgc agcaccgtgc
      661 cgaggcgccg gcgcagatgg cgcaggtcga gtgtcgcgag gtccttcgcg tcgaagcgca
      721 ccgcgcctgc ggagggcgac tcgaaaccca gcagcagtct cagcagcgtc gacttgccgg
      781 agccgctcgc gccgacgatc gcgacgaact cgcccggccg gaccgcgagg tcgatgtcct
      841 gcagcacggc gggcccgccg ggataggcga agctcacctt ctcgagcgcg atcgcgccca
      901 gcgggtcgta gcgctcgccc gcggcgccgg agtcttccgg cggcgtctcg aggatcgggc
      961 gcgcgcggtc ccacacgggc ttgaggttca cgagatcgag cgccgtcgag accagcacgt
     1021 agacggcgcc aaggagggca aagagtgccg catggaacgc gacgaagtcg ccggtcgtca
     1081 tgctcgacgt cggcgccagg cgccaggcga ggaagagcac caggatggtc gccgcgggct
     1141 gcaggagatt cagcgcgacc gattcccagt tggtgaggcg cgcgctcacg ccgttcaggt
     1201 tgcggaagtg gtggtacttg gcataccact gctcgaaggc gcgcgactcg gcggcgcccg
     1261 cgcgcagctt cgcgatcccc gcgaagatct cgaactgcag ggccgagagc ttgccgtcca
     1321 tcgcctcgat gcgcgggccg accttgagcc tcgcgaggcc gatcagcgtc gacaggccga
     1381 tcaccagcac gacgatgccc gaggcggcga gcgtgaggga ggggctgtag gcgatcatca
     1441 gcgccacgtt ggaaacgagg aagagcgagg tgatgaatgt cccgatggtg gagccggtga
     1501 tggtgcgctg gatggtgttg accgagccca tgcgcagggc caggtcgccg ctcgagaaat
     1561 cgcggaagaa ccgcgccggc gcgcagatga cgcggtcgac gagcgcggcc tgcatggcga
     1621 ctccgatgcg cgcctcgaag cgcagcaccg cgagcgagcg cagtacgtcg agcgcgacga
     1681 tcgtcactcc cgcgagcgcg aggcccgcga tgatcgactg caccgtctgc gtcgcgcgcg
     1741 acgggatcgc gtggtcgatg agccatccgg tcgcgatcgg cgtgaagagc cccaccagcc
     1801 ccgcggcgaa tcccgcgatg agcagcaaca gcaggtcctg cccgccgtgg gccgcggcga
     1861 agcgcagcat gtccccggcg ccgagcgcgc gcttgtcgaa gcgccggtag aaggtgaacg
     1921 cgaagggcgc gaggcgcgcg gcgacggact cgtcgacgcg ccattcgacg accttgcccg
     1981 tttccgcatc ggtcgcgcgc atgcggtagc cgccgagatc cggcgtgagc gcgacccatc
     2041 ccgttccttc cgcgagcgac gggctcgtca tcggcgttgg cccccggggc acgcggcgcc
     2101 gatccatgac gcgcgcgatc atcgctccgc ccgattgcct ccaccatccc gcatcgagca
     2161 tgacgcgccg cgcgcgcaac ccggccggca cggccatcgc ctcgatgacc tccgccgcct
     2221 cggaggttgg ggaaattggt gtcaggtacg aattatcgga gacggagtcg ctcctcgata
     2281 attcgta
//
LOCUS       DASVII010000432         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_127613,
            whole genome shotgun sequence.
ACCESSION   DASVII010000432 DASVII010000000
VERSION     DASVII010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127613"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VF309_05645"
     CDS             complement(<1..673)
                     /locus_tag="VF309_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7466104.1"
                     /translation="MSRPPDTRRIEELTLNSSAPPGQLLYDGWILRLLRGKAKRARSV
                     NAVYASTVPLEQKIAYCERLYRDAALPVLFRVTPFSQPQDLDGELERRGFARFDPTAV
                     ELAPIDRGLLGEPEAQPMDLSSWVEAVGQLRQSPIAHRSAHLARLQGMPLAMRAVAVK
                     SQGRVLATGLAILEDDCVGLFDIITADEARRQGHAKRIVASLLRIAWDLGARHAYLQV
                     QEDNVA"
     gene            complement(666..1751)
                     /locus_tag="VF309_05650"
     CDS             complement(666..1751)
                     /locus_tag="VF309_05650"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEX7466105.1"
                     /translation="MAVVHLRGVRKSYDKLEVIHGIDIDIADGEFIVIVGPSGCGKST
                     LLRMIAGLETVTEGEIAIGGRVVNDVEPKDRNIAMVFQNYALYPHMSVYENMSYGLRI
                     RRMPPAEINERVQRAAKILELTGFLDRRPRQLSGGQRQRVAMGRAIVREPAAFLFDEP
                     LSNLDAKLRVQMRLEIQKLHRELKTTSIFVTHDQVEAMTLADRMIVMNAGRAEQIGRP
                     LDVYEDPASLFVAGFIGSPAMNFLPGARAGDEVDVGGGVRFAIPAPLRAAAPEAITVG
                     VRPEHLVPGAGAGGPAIRFKVQTVEALGADSMVHGEFGNGVVVARVEGHSTPKIDEAL
                     TFSVMPGKLYFFDTSTGKRMRPQSGLE"
BASE COUNT          283 a          621 c          598 g          279 t
ORIGIN      
        1 cggcgacatt gtcctcctgc acctggaggt aggcgtggcg cgcgccgagg tcccaagcga
       61 tgcgaagcag gctcgcgacg attcgtttcg cgtggccctg ccggcgcgcc tcgtcggcgg
      121 tgatgatgtc gaagagtccc acgcagtcgt cctcgaggat cgcgagcccg gtcgcgagca
      181 cgcgcccctg cgacttcacc gccacggcgc gcatcgcgag cggcatgccc tgcagccgcg
      241 cgaggtgcgc ggagcgatgc gcgatgggcg attgccgcag ctgccccacc gcctccaccc
      301 atgacgagag atccatgggc tgcgcctcgg gctcgccgag caatccccgg tcgatcggcg
      361 cgagctcgac cgccgtcggg tcgaaacggg cgaagccgcg tcgctcgagc tccccgtcga
      421 gatcctgcgg ctgcgagaac ggcgtcacgc ggaaaaggac gggcaacgcc gcgtcgcgat
      481 agagccgctc gcagtaggcg atcttctgct ccagcggcac ggtcgacgcg tacaccgcgt
      541 tcaccgagcg cgcacgcttc gccttgccgc gcagcagccg caggatccag ccgtcgtaga
      601 ggagctgccc gggcggcgcc gaggaattga gcgtgagctc ctcgatgcgc cgcgtatcgg
      661 gaggcctact caaggccgct ttgcggacgc atgcgcttcc cggtggaagt atcgaagaag
      721 tagagcttgc ccggcatgac ggaaaaggtg agcgcctcgt cgatcttcgg ggtcgagtgc
      781 ccctccacgc gcgcgacgac gactccattc ccgaactcgc catggaccat cgagtccgcg
      841 ccgagagcct ccacggtctg caccttgaag cggattgcgg gaccgcccgc gccggccccg
      901 ggaacgagat gctcgggacg cacgcccacc gtgatcgcct cgggcgcggc cgcgcgcagc
      961 ggcgcgggaa tcgcgaagcg aacgccgccg cccacgtcca cctcgtcgcc ggcgcgtgcg
     1021 cccgggagga aattcatcgc gggcgagccg atgaaccccg cgacgaagag gctcgccgga
     1081 tcctcgtaca cgtccagggg acggccgatc tgctcggccc ttccggcgtt catcacgatc
     1141 atgcggtcgg cgagcgtcat cgcctcgacc tggtcgtggg tcacgaagat gctcgtggtc
     1201 ttcagctcgc ggtgcagctt ctggatctcg aggcgcatct gcacgcgaag cttcgcgtcg
     1261 aggttggaca gcggctcgtc gaagaggaac gcggcgggct cgcgcacgat cgcgcgcccc
     1321 atcgccacgc gctggcgctg gccgccggaa agctggcgcg gccgccggtc gaggaagccc
     1381 gtgagctcga ggatcttcgc cgcgcgctgc acgcgctcgt tgatctcggc gggcggcatg
     1441 cggcggatgc gcaatccgta cgacatgttc tcgtagacgc tcatgtgcgg atagagcgcg
     1501 tagttctgga agaccatcgc gatgttgcgg tccttgggct cgacatcgtt caccacgcgt
     1561 ccgccgatcg cgatctctcc ctcggtcacg gtctccagcc ccgcgatcat tcgcagcaac
     1621 gtggacttgc cgcaacccga cggccccacg atgacgatga actcgccgtc ggcgatgtcg
     1681 atatcgatgc cgtggatgac ctcgagcttg tcgtagctct tcctgacgcc gcgcagatgc
     1741 accacggcca tgctatttct ccgtctcgac aaggccgcgc a
//
LOCUS       DASVII010000433         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_415253,
            whole genome shotgun sequence.
ACCESSION   DASVII010000433 DASVII010000000
VERSION     DASVII010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415253"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VF309_05655"
     CDS             complement(<1..202)
                     /locus_tag="VF309_05655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831244.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit A is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7466106.1"
                     /translation="MAAENLTPSEYIGHHLTFFESAHDGGFWTLNLDTLIVSAILGFV
                     SLGFFWWIVKGATTGVPSKRQAF"
     gene            complement(218..607)
                     /locus_tag="VF309_05660"
     CDS             complement(218..607)
                     /locus_tag="VF309_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX7466107.1"
                     /translation="MRPIRIVLRWQLAVTAVSAAVGMIFWGPDGAASAALGGLINVAA
                     GWVYGWRVSQGEAKSAGEALRTMFRAEALKVLLIFVGLWVVLQNYRDIVHVAFFASFV
                     ITIGVFAAAIAVRDTEEENMPRVSREE"
     gene            complement(728..1588)
                     /locus_tag="VF309_05665"
     CDS             complement(728..1588)
                     /locus_tag="VF309_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7466108.1"
                     /translation="MTTRHKGLGRGLDALLGGAPRTRGEEELSQLPIGVLRPGKYQPR
                     TRMDEVSLAELADSIRSRGVIQPIVVRPIGDSQYEILAGERRWRAARIANLERIPALI
                     REVPDEAALGIGLIENIQREDLNPIEEASGLKRLIEEFKLTHEEVARAIGRSRAGVTN
                     LLRLLELAPAVQAMVQDGAIDMGHARALLALSRTRQIEIAREIAEKGLSVRETERLVQ
                     DAHAPRKIAAPAAAVKLDPDTRRLQEELSERLGAMVRLKPRQGGKGSIVIDYASLDEL
                     EGLLGKLKRA"
     gene            complement(1585..2367)
                     /locus_tag="VF309_05670"
     CDS             complement(1585..2367)
                     /locus_tag="VF309_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7466109.1"
                     /translation="MARIFVIANQKGGVGKTSVAVNLAASLAHYGRRALLIDLDPQGN
                     ATTGSGVDKSACDKTVYGVLLGEHGLRDAIRHCEGGYDIVPSNRELAGAEVELIAMER
                     REYRLREALDGATGGYDFVVIDCPPALNMLTVNGFTAADAVIIPMQCEYYALEGLSDL
                     VGTLRKVKQNLNPRIEIEALVRTMYDPRSTLTVQVSEELKRHYGEKVFDTVIPRNIRI
                     AEAPSFGKPVLLHDPLSKGALAHLQFARELLARNGFEVAEAA"
     gene            complement(2367..>2776)
                     /gene="rsmG"
                     /locus_tag="VF309_05675"
     CDS             complement(2367..>2776)
                     /gene="rsmG"
                     /locus_tag="VF309_05675"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX7466110.1"
                     /translation="DVGTGPGLPGIPIAIALPGSRVTLLDSSHKKCAFLQQAKTELGL
                     ANVEIVCDRVENWKPREKFEAVVSRAFSDLGDFVAQAQHLLAPGGRLIAMKGVYPFDE
                     IARVPVTHRVAKVLELHVPRLDAKRHLVFVEAA"
BASE COUNT          472 a          940 c          897 g          467 t
ORIGIN      
        1 cgaaggcctg gcgcttcgat ggcacgcccg ttgtcgcgcc cttgacgatc caccagaaga
       61 acccgaggct cacgaacccc aggatcgcgg acacgatcag cgtatcgagg ttgagcgtcc
      121 agaagccacc gtcatgggcg ctctcgaaga acgtcaggtg atgcccgatg tattccgaag
      181 gggtcaggtt ctctgcggcc atcgacgagt ctttcggtca ttcctcccgg gaaacccggg
      241 gcatgttttc ttcttctgtg tcgcgcaccg cgatggcggc ggcgaacacc cctatcgtga
      301 tcacgaagct cgcgaagaac gcgacgtgca cgatgtccct gtagttctgg agcacgaccc
      361 agagcccgac gaagatcagg agcaccttga gcgcttccgc cctgaacatc gtccgcagcg
      421 cctcccctgc cgactttgcc tcaccctgcg acactcgcca gccgtacacc caccccgcgg
      481 cgacgttgat cagtcccccg agggccgcgg atgccgcgcc gtcaggaccc cagaagatca
      541 ttcccaccgc cgccgagacc gccgtgacgg caagctgcca ccgcagaacg atgcggatgg
      601 gcctcatcgg gggtttgaag acgcccaccc tgttgtcgaa cacgatgaat gcccatgaag
      661 caaaaccgct caattatagc gagggcggct acccagcgtc aaaagttgtc gcggtgcagc
      721 atcggtctca cgcgcgcttc agtttcccga gcaatccctc gagctcatcc aggctcgcgt
      781 agtcgatgac gatcgagccc ttgccgccct ggcgcggctt gaggcgcacc atcgcgccga
      841 gcctctccga gagctcctcc tgcaggcgtc gcgtgtcggg atcgagcttc accgccgcgg
      901 cgggcgccgc gatcttgcgg ggagcatgag cgtcctggac gaggcgctcg gtctcgcgaa
      961 ccgacaatcc cttttcggcg atctcgcgcg cgatctcgat ctgccgcgtc ctcgacaacg
     1021 cgagcaaggc ccgcgcgtgc cccatgtcga tcgcgccgtc ctgcaccatt gcctgaaccg
     1081 ccggcgcgag ctcgagcaag cgcagcaaat tcgtcacgcc tgcacgcgag cgcccgatcg
     1141 cgcgcgcgac ctcctcgtgt gtcagcttga actcctcgat gaggcgcttc agccccgagg
     1201 cctcctcgat gggattgagg tcctcgcgct ggatgttctc gatcaggccg attcccagcg
     1261 cggcctcgtc ggggacttcg cgaatcaacg ccggaatgcg ctcgagattc gcgatgcgcg
     1321 ccgcgcgcca gcggcgctcg ccggcgagga tctcgtactg cgagtcgccg atgggccgga
     1381 ccacgatcgg ctgtatcacg ccgcgggagc gaatcgaatc ggcgagctcg gcgagcgaga
     1441 cttcatccat ccgggtgcgc ggctggtact tgccgggacg cagcacgcct atgggaagct
     1501 gggagagctc ctcctcgccg cgagtgcggg gcgccccgcc gagaagcgcg tcgaggccgc
     1561 ggccgaggcc cttgtgccgg gtggtcatgc cgcctccgcg acttcgaagc cgttgcgcgc
     1621 cagaagctcg cgcgcgaatt gcagatgcgc gagcgcgccc ttcgacaggg ggtcgtgcag
     1681 cagcacgggc ttgccgaacg agggagcctc ggcgatgcgg atgttgcgcg ggatcacggt
     1741 atcgaagacc ttctcgccgt aatggcgctt cagctcctcg gagacctgca cggtgagggt
     1801 gctgcgcggg tcgtacatcg tgcgcacgag ggcctcgatc tcgattcgcg gattcaggtt
     1861 ctgcttcacc ttgcgaagcg tgccgacgag gtcggaaagg ccctcgagcg cgtaatactc
     1921 gcactgcatc gggatgatga cggcatcggc cgcggtgaac ccgttcaccg tgagcatgtt
     1981 gagcgcgggc gggcaatcga tcaccacgaa gtcgtagccg cccgtggcgc catcgagcgc
     2041 ttcgcgcagg cgatattcgc ggcgctccat cgcgatcagc tcgacctcgg cccccgcgag
     2101 ctcgcggttg gaaggaacga tgtcgtagcc gccttcgcaa tgccggatcg cgtcgcgcag
     2161 gccatgctcg cccagcagga ctccgtagac ggtcttgtcg cacgcgctct tgtccacgcc
     2221 gctgcccgtg gtcgcgttgc cctgtggatc caggtcgatg aggagcgcgc ggcgcccgta
     2281 atgcgcgagg ctcgcggcaa ggttcaccgc caccgaggtc ttgccgacgc cgcccttctg
     2341 gttggcgatc acgaagatgc gcgccatcac gcggcctcga cgaagacgag atggcgcttg
     2401 gcgtccaggc gagggacgtg caactcgagc accttcgcga cacggtgagt aaccggcacg
     2461 cgtgcgatct catcgaaggg gtagacgccc ttcatcgcga tgagccggcc gccgggggcg
     2521 aggaggtgct gcgcctgcgc gacgaagtcg ccgagatcgg aaaacgcgcg cgataccacg
     2581 gcttcgaatt tctcacgagg cttccagttc tccacccgat cgcaaacaat ttcgacattg
     2641 gcgaggccga gctcggtctt cgcctgctgg aggaacgcgc acttcttgtg gctcgaatcg
     2701 aggagcgtca cgcggctgcc gggcagcgcg atcgcgatgg gaatccccgg aagccccggg
     2761 cccgttccca cgtcga
//
LOCUS       DASVII010000434         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_523021,
            whole genome shotgun sequence.
ACCESSION   DASVII010000434 DASVII010000000
VERSION     DASVII010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523021"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..669
                     /locus_tag="VF309_05680"
     CDS             121..669
                     /locus_tag="VF309_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX7466111.1"
                     /translation="MKKPNAKKRQDLEARRGAPLATPTDLKKAATKDIAGAMNAILAD
                     VFALYMKTKNFHWHMSGPHFRDYHLLLDEQADQLYAMTDPIAERVRKVGGSTLRSIGD
                     IGRTQRVKDNDARYVEPVDMIAELREDNKALASRLREAHNVCDEHHDIATASLIEVWI
                     DETERRTWFLFEASRMADSSGH"
     gene            675..1484
                     /locus_tag="VF309_05685"
     CDS             675..1484
                     /locus_tag="VF309_05685"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX7466112.1"
                     /translation="MAAATSPARRGTAPALFISHGAPTFALEPGLLGPQLTKLGGQLN
                     GLAAVLVVSPHWQTPGIRVMTTAAPETIHDFGGFPRALYEMRYPARGEPAIAAEVALL
                     LREAGFDVALDEKRGLDHGTWVPLRYLLPRADVPVFQVSMPMDLDPAGAFRLGHALAP
                     LRERGVLIVGSGSLTHNLYEFRQDVKDPEYARDFAQWVAKAVVARDAERLVDYRRLAP
                     HAERAHPTDEHFLPLLVAMGASSETEIPQHIEGGMTYGTLSMDSYGWGLPS"
     gene            1542..>1765
                     /locus_tag="VF309_05690"
     CDS             1542..>1765
                     /locus_tag="VF309_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7466113.1"
                     /translation="MKDTPGGWPRISSAIYCRDPARMIDWLCDAFGFEVRIKVEGDGG
                     RIEHSELTFGEGLIMVGEARDKYPYRRAPT"
BASE COUNT          322 a          588 c          578 g          277 t
ORIGIN      
        1 catttttctc atagctgcct cctctcaggc aagatactct ttcccgatgg gatgcggtgc
       61 atcgaccgga agaaccatga atgccgacct ccaacgttgt tgatcgaaag gacgaaagcc
      121 gtgaagaaac ccaatgcgaa gaagaggcag gatctcgaag ccaggcgcgg cgcgccgctc
      181 gccaccccga ccgacctgaa gaaggcggcg acgaaagaca tcgccggcgc gatgaacgcc
      241 atcctcgcgg acgtgttcgc tctctacatg aagaccaaga atttccattg gcacatgagc
      301 gggccccact tccgcgacta ccacctgctg ctcgacgagc aggccgacca gctctacgcg
      361 atgaccgatc cgatcgccga gcgcgtccgc aaggtgggcg gatcgacgct caggtccatc
      421 ggcgacatcg gccgcacgca gcgcgtgaag gacaacgacg cgcgatacgt ggagcccgtg
      481 gacatgatcg ccgagctgcg cgaggacaac aaggcgctcg cctcgcgcct gcgcgaggcc
      541 cacaacgtct gcgacgagca ccacgacatc gccaccgcga gcctgatcga ggtctggatc
      601 gacgagacgg aacggcgcac gtggttcctg ttcgaggcga gccgcatggc ggacagctcg
      661 gggcattgag cgggatggcg gccgcgacct ccccagcgcg gcgcggcacg gcgcccgcgc
      721 ttttcatctc ccacggcgcg ccgaccttcg ccctcgagcc ggggctcctc ggcccgcaac
      781 tgacgaagct gggcgggcaa ttgaacgggc tcgcggccgt gctcgttgtt tcgccgcact
      841 ggcaaacgcc gggaatccgg gtcatgacga ccgcggcgcc cgagacgatc cacgacttcg
      901 gcggctttcc gcgcgcgctc tatgaaatgc gatatccggc ccgaggcgag cctgcgatcg
      961 ccgccgaggt cgcgctcttg ctccgcgagg ccggcttcga cgtcgcgctc gacgagaagc
     1021 gcggattgga tcacgggacc tgggtgccgc ttcgttacct gttgccgcga gcggacgtgc
     1081 cggtcttcca ggtctcgatg ccgatggacc tggatccggc cggcgccttc cggctcggcc
     1141 acgcgctcgc gccgctacgc gaacgtggcg tgctcatcgt gggctcgggc agcctcaccc
     1201 acaacctcta cgagtttcgc caggatgtga aggatcccga gtacgcgcgc gatttcgcac
     1261 agtgggtcgc gaaggccgtc gtggcgaggg acgcggagcg cctcgtggac taccgaaggc
     1321 tcgcgcccca cgccgagcgc gcccatccca ccgacgagca cttcctgccg ctcctggtcg
     1381 cgatgggcgc gagctcggaa accgagatcc cccagcatat cgagggcgga atgacctacg
     1441 ggaccctgtc gatggattcg tatgggtggg gattgccgtc ttgacaggcg cgttaattat
     1501 taacacaatc gttaataatt aacggcgatg gagggcaggc aatgaaggac actcccggcg
     1561 gctggccgcg catctcgtcg gccatttact gccgcgatcc ggcgcggatg atcgactggc
     1621 tgtgcgacgc cttcggcttc gaggtgcgga tcaaggtcga gggcgacggc gggcgcatcg
     1681 agcactccga gctcaccttc ggcgaggggc tgatcatggt cggcgaggcc cgcgacaagt
     1741 acccctaccg gcgggcgccg accca
//
LOCUS       DASVII010000435         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_163311,
            whole genome shotgun sequence.
ACCESSION   DASVII010000435 DASVII010000000
VERSION     DASVII010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163311"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF309_05695"
     CDS             complement(<1..465)
                     /locus_tag="VF309_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX7466114.1"
                     /translation="MKILFLTHAFNSLAQRLFVEIAAAGHEVSIEFDVNDAVTRQAVA
                     LAKPDAIVAPFLKRAIPEDIWSRIPCLIVHPGPPGDRGPSSLDWAIQEGERDWGVTVL
                     QAVGEMDAGPVWAHAGFPMRLASKGSLYRREVTEAAVTCIAEAVEKMARGERP"
     gene            complement(470..1519)
                     /gene="hypE"
                     /locus_tag="VF309_05700"
     CDS             complement(470..1519)
                     /gene="hypE"
                     /locus_tag="VF309_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX7466115.1"
                     /translation="MSAVRKDYVRPVDFKRGVVDMTHGSGGRAMAQLVEELFVAAFDN
                     RWLDEMNDHARLAMPPGRMVIATDSHVVSPLFFPGGDIGCLSVHGTVNDVAMSGARPL
                     YLTAGFILEEGYPLADLKRIVESMAAAAREAGVPIVTGDTKVVEKGKGDGVFINTTGV
                     GVVAEGIEISGDRARPGDAILVSGTMGDHGVAIMSLRENLTFETTIRSDTAALHGLVA
                     AMIAAVPRIHCLRDPTRGGLATTLNEIARQSKVGMRLKETAIPVKPEVSAACELLGLD
                     PLYVANEGKLVAICAADDAAKLLEAMRAHPLGRDAALVGHVVADAHHFVQMETGFGGR
                     RMVDWLAGEQLPRIC"
BASE COUNT          241 a          587 c          539 g          238 t
ORIGIN      
        1 gggacgctct ccgcgcgcca tcttctccac cgcctcggcg atacaggtca ccgccgcctc
       61 ggtcacctcg cggcgataga ggcttccctt ggacgcgagg cgcatcggga acccggcgtg
      121 ggcccacacc ggacccgcat ccatctcgcc gaccgcctgc agcaccgtga cgccccagtc
      181 gcgctcgcct tcctggatcg cccagtcgag cgacgacggc ccgcgatcgc ccggcgggcc
      241 gggatggacg atgaggcacg ggatgcgcga ccagatgtcc tcgggaatgg cgcgcttcaa
      301 gaacggcgcc acgatcgcgt cgggcttcgc cagcgccact gcctggcgcg tcaccgcgtc
      361 gttcacgtcg aattcgatgc tcacctcgtg gccggcggcg gcgatctcga cgaacaggcg
      421 ctgcgcgagc gagttgaagg cgtgcgtgag gaaaaggatc ttcatcgctt cagcagatcc
      481 gcggcagttg ctcgccggcg agccagtcca ccatgcgccg gccgccgaag ccggtttcca
      541 tctgcacgaa gtggtgcgcg tccgcgacga cgtggcccac gagggcggcg tcgcgcccga
      601 gcgggtgcgc gcgcatcgcc tcgaggagct tcgccgcatc gtccgcggcg cagatcgcga
      661 ccagcttgcc ctcgttggcg acgtacagcg gatcgagccc aaggagctcg cacgcggccg
      721 agacttccgg cttcacgggg atcgcggtct ccttcaggcg catgcccacc ttcgactggc
      781 gcgcgatctc gttcagcgtg gtcgccaggc ccccgcgcgt cggatcgcgc aggcaatgga
      841 tgcgcgggac cgcggcgatc atcgctgcga ccagtccatg cagcgccgcg gtatccgacc
      901 ggatggtggt ctcgaacgtg aggttctcgc gcagcgacat gatcgccacg ccatggtcgc
      961 ccatggtgcc ggacaccagg atcgcgtcgc cgggccgcgc gcggtcgccc gagatctcga
     1021 tgccctcggc gacgacgccc acgccggtgg tgttgatgaa gacgccatcg cccttgccct
     1081 tctcgaccac cttggtgtcg ccggtcacga tcggcacgcc cgcctcgcgc gccgccgcgg
     1141 ccatcgactc gacgatgcgc ttcaagtcgg caagcggata cccctcctcg aggatgaagc
     1201 ccgcggtgag atagagcggc ctcgccccgg acatcgccac gtcgttcacg gtgccgtgca
     1261 ccgagaggca gccgatgtcc ccgccgggga agaagagcgg cgacaccacg tggctgtcgg
     1321 tcgcgatcac catgcgcccg ggcggcatcg cgaggcgcgc gtggtcgttc atctcgtcca
     1381 gccaccggtt gtcgaaggcg gcgacgaaga gctcctcgac caactgcgcc atggcccgcc
     1441 cgccgctgcc gtgggtcatg tcgacgacgc cgcgcttgaa gtcgaccggg cggacgtagt
     1501 ccttgcgaac cgcgctcatg cggcctcctc ggcggccggc tcggagaacc ggccgtacgt
     1561 gtagtgcgcg gcgcacgccc cttccgacga caccatgcag gagcc
//
LOCUS       DASVII010000436         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_235559,
            whole genome shotgun sequence.
ACCESSION   DASVII010000436 DASVII010000000
VERSION     DASVII010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235559"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="nuoG"
                     /locus_tag="VF309_05705"
     CDS             complement(<1..885)
                     /gene="nuoG"
                     /locus_tag="VF309_05705"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX7466116.1"
                     /translation="MITLEVDGKSVQVENGKTVMDAATKLGIFVPHFCYHKKLSIAAN
                     CRMCLVQVEKVPKPLPACATPATEGMKAFTHSDSAIQAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGPSASRYTEAKRVVVNKNLGPLISTDMTRCIQCTRCVRFGQEIA
                     GVMELGMAGRGEHSEILTFVGRTVDSELSGNMIDICPVGALTSKPFRYSARAWELSRR
                     KSVSAHDSLGSNLLVQCKQERVMRVLPIESESINEVWISDRDRFAYSGLDSEERLLRP
                     MVKRAGQWHEVDWPEALEA"
     gene            complement(900..>1506)
                     /locus_tag="VF309_05710"
     CDS             complement(900..>1506)
                     /locus_tag="VF309_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX7466117.1"
                     /translation="TKLFSVTGNVEKPGNYEVKLGTPFAKLLEMAGGMRGGKKLKGVI
                     PGGSSMPVLTAEVMMGLDMDYDSIAKAGSFLGSGAVIVMDEDTCMVKAAERLAYFYFE
                     ESCGQCTPCREGTGWLYRILNRIEHGQGRMEVLDLLLNLSDNIQGRTICALGDAAAMP
                     TRAFVKVFRDEFVHHIENKCCNVKPGGYSSANPDLHKMAAD"
BASE COUNT          240 a          490 c          501 g          275 t
ORIGIN      
        1 ggcttcgagc gcctcgggcc agtcgacctc gtgccattgg ccggcgcgct tcaccatcgg
       61 gcgaagcagg cgctcctcgg agtcgaggcc gctgtacgcg aagcggtcgc ggtccgagat
      121 ccagacctcg ttgatcgact cgctctcgat gggcagcacg cgcatcacgc gttcctgctt
      181 gcattgcacc aggaggttcg agccgagcga atcgtgcgcg ctcacggact tgcggcgcga
      241 gagctcccag gcgcgcgcgg aatagcggaa cggcttcgag gtgagcgcgc ccaccgggca
      301 gatgtcgatc atgttgccgg agagctcgga atccaccgtg cgtccgacga acgtgaggat
      361 ctcggagtgc tcgccgcggc cggccatgcc gagctccatc acgcccgcga tctcctgccc
      421 gaagcgcacg cagcgcgtgc actggatgca gcgcgtcatg tcggtggaaa tcagcgggcc
      481 gaggttcttg ttcaccacga cgcgcttggc ttccgtgtag cgcgaggccg agggaccgta
      541 gcccaccgcg aggtcctgca gctggcattc gccgccctgg tcgcagatcg ggcagtcgag
      601 cggatggttg atgaggagga actccatcac gcccttctgg gcctggatcg cggaatccga
      661 gtgcgtgaag gccttcatgc cctcggtggc gggcgtggcg cacgcgggca gcggcttggg
      721 caccttctcg acctgcacca ggcacatgcg gcagttggcc gcgatcgaca atttcttgtg
      781 gtagcagaag tgcggcacga agatgccgag cttggtcgcg gcgtccatca ccgtcttgcc
      841 gttctcgacc tggacggatt tgccgtcgac ctcgagagtg atcatcttca cttcgctcat
      901 cagtcggccg ccattttgtg caagtcgggg ttggccgagc tgtatccgcc cggcttcacg
      961 ttgcagcact tgttctcgat gtggtgcacg aactcgtcgc ggaacacctt cacgaaggcg
     1021 cgcgtcggca tcgccgcggc gtcgcccagc gcgcagatgg tgcggccctg gatgttgtcg
     1081 gagaggttga ggaggagatc gaggacctcc atgcggccct ggccgtgctc gatgcgattg
     1141 aggatccggt agagccagcc cgtgccctcg cggcacggcg tgcactgccc gcacgactcc
     1201 tcgaagtaga agtacgcgag acgctcggcg gccttcacca tgcaggtgtc ctcgtccatg
     1261 acgatcaccg cgcccgagcc gaggaacgag cccgccttgg cgatcgagtc gtagtccatg
     1321 tcgaggccca tcatcacttc cgcggtgagc accggcatcg acgatccgcc ggggatgaca
     1381 cccttcagct tcttgccgcc gcgcatgccg cccgccatct cgaggagctt cgcgaacggg
     1441 gtgccgagct tcacctcgta gttgccgggc ttctcgacgt tgcccgtcac cgagaagagc
     1501 ttggtg
//
LOCUS       DASVII010000437         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_379722,
            whole genome shotgun sequence.
ACCESSION   DASVII010000437 DASVII010000000
VERSION     DASVII010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379722"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF309_05715"
     CDS             <1..278
                     /locus_tag="VF309_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="octanoyltransferase"
                     /protein_id="HEX7466118.1"
                     /translation="YRIAGKRRAGMPGVYVEGAKIAAIGLRIARGCSYHGLALNVAPD
                     LEPFTRIDPCGYPGLASTRMADHGVRDKISAVQQSLADVLSRCLTPS"
     gene            260..1195
                     /gene="lipA"
                     /locus_tag="VF309_05720"
     CDS             260..1195
                     /gene="lipA"
                     /locus_tag="VF309_05720"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7466119.1"
                     /translation="MPDSELKIQQKRSLKTARIPIKVVASEPLRKPEWIRVRAGGGER
                     FTEIKRILREASLHTVCEEASCPNIGECFGHGTATFMIMGSLCTRRCPFCDVAHGRPE
                     PLDADEPRHLGETIAKMRLKYVVVTSVDRDDLRDGGAQHFVDCIRAVRELSPATRIEI
                     LTPDFRGRLDRALAILDAAPPDVMNHNLETVPRLYRQARPGSDYAHSLRLLRDFKARH
                     PGVPTKSGLMVGLGETNDEILEVMRDMRAHGIDMLTLGQYLQPSKHHLSVARFAHPDE
                     FRMFEAKAAEMGFANAACGPMVRSSYHADRQAQDV"
     gene            1209..1487
                     /locus_tag="VF309_05725"
     CDS             1209..1487
                     /locus_tag="VF309_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7466120.1"
                     /translation="MKIRASIWSVAILLAAYAGSAGAEIYRCIAGGSVSYQEFPCPAA
                     SDAATMPIGADFPAVNLMERERADAAPYYVAARPLRPGLRRAWPIAIR"
     gene            1647..>2105
                     /locus_tag="VF309_05730"
     CDS             1647..>2105
                     /locus_tag="VF309_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7466121.1"
                     /translation="MDRFKELTTFVEVAQRGSLSAAAREEGITPAMVGRRIDQLEERL
                     GVKLFKRSTRKVTLTPEGTTFYEDCHRILGELRSAEDALTVGVKAASGRLIVTAPTAF
                     GRKHIAPHLSAFIAEHPNLAITLHLSERLVDLKNERFDLAIRIADLKSADL"
BASE COUNT          358 a          741 c          663 g          343 t
ORIGIN      
        1 gataccgaat cgccggaaag cggcgtgccg gcatgccggg cgtctacgtg gaaggcgcga
       61 agatcgcggc gatcgggctt cgcatcgcgc gcggctgcag ctatcacggg ctcgctttga
      121 acgtggcgcc ggacctcgag cccttcacgc gcatcgatcc gtgcgggtac ccgggcctcg
      181 cctccacgcg catggccgac catggagtgc gtgataaaat tagtgcggtg caacaatcac
      241 tggccgacgt cctctcgaga tgcctgactc cgagctgaag atccagcaga aacgctcgct
      301 caagacggcg cgcatcccca tcaaggtggt tgcctccgag ccgttgcgca agcccgaatg
      361 gatccgcgtg cgcgcgggcg gcggcgagcg cttcaccgag atcaagcgca tccttcgcga
      421 agctagcctg cacaccgtct gcgaggaagc gtcctgcccg aacatcggcg agtgcttcgg
      481 ccacggcacc gcgaccttca tgatcatggg gagcctgtgc acgcgccgct gccccttctg
      541 cgacgtggcg cacggccggc ccgagcccct ggacgcggac gagccgcgcc atctcggcga
      601 gaccatcgcg aagatgcgcc tcaagtacgt ggtcgtcacc agcgtcgatc gcgacgattt
      661 gcgcgatggc ggcgcgcagc atttcgtcga ttgcatccgc gccgtgcgcg agttgtctcc
      721 ggccacgcgc atcgagatcc tcacgccgga tttccgcggc cggctcgatc gcgcgctggc
      781 gatcctcgac gcggcgcctc ccgacgtgat gaaccacaac ctggagaccg tgccgcgcct
      841 ctaccggcag gcgcgccccg gctccgacta cgcccattcg ctgcggctcc tgcgcgactt
      901 caaggcgcgc caccccggag ttcccaccaa gtccgggctg atggtggggc tcggcgagac
      961 caacgacgag atcctcgagg tgatgcgcga catgcgcgcg catggcatcg acatgctcac
     1021 gctcgggcaa tacctgcagc cctcgaaaca ccacttgtcg gtggcgcgct tcgcgcaccc
     1081 ggacgagttc cggatgttcg aggcgaaggc cgcggagatg ggattcgcga atgccgcttg
     1141 cggacccatg gtgcgttcca gttatcatgc ggatcgtcag gcccaggacg tgtaaagcgg
     1201 ggggtcccat gaagattcgc gcatcgatct ggagtgtcgc cattctcctg gcggcgtatg
     1261 cggggagcgc cggcgcggaa atctaccggt gcatcgccgg cgggtccgtc agctaccagg
     1321 aatttccctg cccggccgcc tccgatgccg ccaccatgcc catcggcgcc gacttccccg
     1381 cggtgaacct gatggagcgc gagcgcgcag acgcagcccc gtactacgtg gccgcgcggc
     1441 cgttgcgccc gggcttgcga cgcgcctggc cgatcgccat tcgatagcgc ggtaagcggg
     1501 gagcgtcacg cgccccgcct cactgcattc cccatgcagt gcgcatcccc cctcggggat
     1561 cagtgagtca ggttgcagtc cattacctaa ctcgcataca ctcaatcagc taaaaactag
     1621 aatcgtttga tactccggga ggagaggtgg accgcttcaa ggagctcacg actttcgtcg
     1681 aggtcgcgca acgcggcagc ctttccgcgg ccgcgcgcga ggagggcatc actccggcga
     1741 tggtcgggcg gcgcatcgac cagctcgagg agcgcctcgg cgtgaagctc ttcaagcgct
     1801 cgacgcgcaa ggtcacgctc acgcccgaag ggaccacctt ctacgaggat tgtcatcgca
     1861 tcctcgggga attgcgctcc gcggaggacg ccctcaccgt cggcgtcaag gccgcatcgg
     1921 gccggctcat cgtcaccgct cccaccgcct tcggccgcaa gcacatcgcg ccgcaccttt
     1981 ccgcgttcat cgccgagcat cccaacctgg cgatcacgct gcacctgtcg gagcgcctcg
     2041 tcgatctcaa gaacgagcgc ttcgacctcg cgatccgcat cgcggacctc aagagcgccg
     2101 acctc
//
LOCUS       DASVII010000438         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_703082,
            whole genome shotgun sequence.
ACCESSION   DASVII010000438 DASVII010000000
VERSION     DASVII010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703082"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1970
                     /locus_tag="VF309_05735"
     CDS             39..1970
                     /locus_tag="VF309_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7466122.1"
                     /translation="MRNAACVGLVALAPAFARADAPGDADVLAARDAALRGQWKQVET
                     YRARLAGHILEAYPAYWLLVGNIERSDPRDVRAFLTRHADSPLSEGLRRDWLKALGAA
                     GSWELFRAEYPRMLGDDVEVACYSFQERLARGDTEAIAEARALFISARETGGACDPVF
                     AAAVAANAVSEADAWDRVRRLLSSGNVKEAKRAGALLPYRVSIVDKSLDGVARDAGRF
                     LTHEKLGKNPNRASQETLVFAIERLARNKPEEAAERLTAIAPRLTADAAAYAWGQVAW
                     QAALAHQPRALEWYALARNTPLTDSQIAWRARAALRAADWKEVLASIQMLSPTQARES
                     NWRYWRARALRNLGAPEAADALLKGLAGQQNFYGLIAADELGIAATPDWNGWRASEAD
                     LDRMRGIDAIQRSLALYRLGMDNEALREWLWAIRGLDDRGLLAAAELARLANEPDRAI
                     NTADRTLQIHDFAQRFPIPHREALGAAARQWDLDEAIVYGIVRQESRFMPEARSPVGA
                     RGLMQLMPATARWVARQIAISPFRPEMLVRPDVNAQMGSYYFRRVLDDLGHPILATAA
                     YNAGPGRARRWRDERPLEGAIYIETIPFNETRDYVKKVFTNAWFYRHRLTGKAASMRE
                     LLGTVPGRAGEPAVAANIP"
     gene            1972..2898
                     /locus_tag="VF309_05740"
     CDS             1972..2898
                     /locus_tag="VF309_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7466123.1"
                     /translation="MDIESLCIIGGTGFVGRSIAEQACARGLRVRVVTRSAPRARPLL
                     VLPTLEAIVADPNDEASLGRCLENMDAAVNLAGILHERRGATYESVHAELPRKIAQAC
                     RVAGVRRFLHMSALGASAEGPSAYLRSKARGEAAVREGAAARQWDLDEAIVYGIVRQE
                     SRFMPEARSPVGARGLMQLMPSTARWGARQIAITPYRPEMLVRPDVNALMGSYYFRRV
                     LDDLGHPILATAAYNAGPGRARRWRDARPLEGAIYAETIPFNETRDYVKKVFTNAWFY
                     RHRLTGKAASMRELLGTVPGRANEPAVAANIP"
BASE COUNT          439 a         1057 c         1041 g          407 t
ORIGIN      
        1 cagcggtggc tcgaatggcc ttgacttgca tcaagagggt gagaaatgcg gcatgcgtgg
       61 gactcgtggc gctcgcgccc gcgttcgcgc gcgccgacgc cccgggcgat gccgatgtcc
      121 tggccgcccg ggacgccgcg ctgaggggcc agtggaagca ggtcgagacc tatcgcgcgc
      181 gcctcgcggg gcacatcctc gaggcctatc ccgcgtactg gctcctcgtc ggaaacatcg
      241 agcgcagcga cccacgcgac gtgcgggcct ttctcacgcg ccatgccgac tcgccgctga
      301 gcgaggggct gcgccgcgac tggctgaagg cgctgggcgc cgcgggctca tgggagctct
      361 tccgcgccga gtatccgcgc atgctcggcg acgacgtcga ggtcgcgtgc tattcgttcc
      421 aggagcgcct ggcgcgaggc gacaccgagg cgatcgcgga ggcgcgcgcg ctcttcatat
      481 ccgcgcggga gaccggcggg gcctgcgatc ccgtgttcgc cgccgcggtc gccgcaaacg
      541 cggtttccga agccgacgcg tgggatcgcg tgcgaaggct cctctcgtcg ggaaacgtga
      601 aggaagccaa gcgcgccggg gcgctgcttc cataccgcgt ctccatcgtc gacaagtcgc
      661 tcgacggcgt ggcccgcgac gccggccgct tcctcaccca cgagaagctc ggcaagaatc
      721 ccaaccgcgc atcgcaggag acgctggtct tcgcgatcga gcgcctcgcg cgcaacaagc
      781 ccgaggaagc cgccgagcgg ctcacggcca tcgctccgcg ccttaccgcg gacgcagcgg
      841 cgtacgcttg gggacaagtc gcatggcaag cggcgcttgc gcaccagccg cgggcgctgg
      901 agtggtacgc actcgcgcgc aacacgccgc tcaccgattc gcagatcgcg tggagggcgc
      961 gcgccgcgtt gcgcgccgcc gattggaagg aagtgctcgc gtcgatccag atgctctccc
     1021 cgacgcaagc gcgcgagtcc aactggcgct attggcgcgc ccgcgcgttg cgcaacctcg
     1081 gcgcgcccga ggccgccgac gcgctgctga aggggctcgc cggccagcag aacttctacg
     1141 ggctcatcgc cgcggacgag ctgggaatcg cggcgacgcc cgactggaac ggctggcggg
     1201 cgagcgaggc ggatctcgat cgcatgcggg gcatcgatgc gatccagcgc tcgctcgcgc
     1261 tctaccggct cggcatggac aacgaggcgc tgcgcgaatg gctgtgggcc attcgcgggc
     1321 tcgacgaccg cggcctcctc gcggccgcgg agctcgcgcg cctcgccaac gagcccgatc
     1381 gcgcgatcaa caccgccgat cgcaccctgc agatccacga cttcgcgcag cgctttccca
     1441 tcccgcaccg cgaagcgctc ggcgcggcgg cgcggcagtg ggatctcgac gaggcgatcg
     1501 tgtacggcat cgtgcgccag gagagccgct tcatgcccga ggcgcgctcg cccgtcggcg
     1561 cgagggggtt gatgcagctg atgcccgcca ccgcgcgctg ggtcgcgcgc cagatcgcga
     1621 tctcgccgtt ccgccccgag atgctggtgc ggcccgacgt gaacgcgcag atgggaagct
     1681 actatttccg ccgcgtgctg gacgacctgg gccatccgat cctcgccacg gcggcctaca
     1741 acgccgggcc ggggcgcgcg cgccgatggc gcgacgagcg tcccctcgaa ggcgcgatct
     1801 acatcgagac cattcccttc aacgagacgc gcgactacgt gaagaaggtg ttcacgaacg
     1861 cgtggttcta ccgccaccgc ctcacgggca aggcggcaag catgcgcgag ctgctgggca
     1921 ccgtgcccgg ccgcgcgggc gagcccgccg tcgccgcgaa catcccctga catggatatc
     1981 gagagcctct gcatcatcgg cggcaccggg ttcgtgggcc gctccatcgc cgagcaggcg
     2041 tgcgcgcggg gcctgcgcgt gcgcgtggtc acgcgcagcg cgccgcgcgc gcgtccactc
     2101 ctcgtgctgc ccacgctcga ggcgatcgtg gccgatccca acgacgaggc gtcgctcggg
     2161 cgatgcctcg agaacatgga cgcggccgtc aatctcgcgg gaatcctcca cgagcgccgc
     2221 ggcgcgacgt acgagtcggt gcatgcggag ctgccgcgca agatcgcgca agcctgccgc
     2281 gtcgccggcg tgcgccgctt ccttcacatg agcgcgctcg gcgcgtcggc cgaaggaccg
     2341 agcgcgtacc ttcgctcgaa ggcccggggc gaggcggcgg tccgcgaggg cgcggcggcg
     2401 cggcagtggg atctcgacga ggcgatcgtg tacggcatcg tgcgccagga gagccgcttc
     2461 atgcccgagg cgcgctcgcc cgtgggcgcg aggggattga tgcagctcat gccttcgacc
     2521 gcgcgctggg gcgcgcgcca gatcgcgatc acgccgtatc gccccgagat gctggtgcgc
     2581 cccgacgtga acgcgttgat gggcagctac tatttccgcc gcgtgctcga cgacctcggc
     2641 catccgatcc tcgccaccgc ggcgtacaac gccgggccgg gccgcgcgcg ccgctggcgc
     2701 gacgcgcggc cgctggaggg cgcgatctac gctgagacca ttcccttcaa cgagacgcgc
     2761 gactacgtga agaaggtgtt caccaacgcg tggttctacc gccaccgcct gaccggcaag
     2821 gcggcgagca tgcgcgagct gctgggcacc gtgcccggcc gcgccaacga gccggccgtc
     2881 gccgcgaaca ttccctgaca tggacatcga gagcgtctgc ctcatcggcg gcaccggatt
     2941 cgtg
//
LOCUS       DASVII010000439         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_199296,
            whole genome shotgun sequence.
ACCESSION   DASVII010000439 DASVII010000000
VERSION     DASVII010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199296"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VF309_05745"
     CDS             <1..960
                     /locus_tag="VF309_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7466124.1"
                     /translation="ELAACVLCLAVASDARALGHSPGVWEKAANPILDSNDETHREVQ
                     GLLMQMMPTPGDYWESGQGREQLRTALEKLDEARADTAPDVRLRFDLGEVAHNLRDYA
                     RCSRALQSALAEAPDDPQATHAYFMLGICHAKLQQPEKEVTCYDEFLRRETNTLGRAQ
                     ALSNRAEAQMLMHRLPPAIADYRASLALQPDMALTHFGLAVALDRGGDAPGAMVEAKA
                     AATYDPLDQDLTRADVFFMPPYDLSFYEALNAMARAQMVDDPAASVLWWETAVAKWME
                     YVALAASSDPWIALAKAHQSSCERQLTAAKKKLARPLKTKKKG"
     gene            complement(957..>1960)
                     /locus_tag="VF309_05750"
     CDS             complement(957..>1960)
                     /locus_tag="VF309_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466125.1"
                     /translation="FAILVATALHVPAGGLILFAGWSATLALALRYGPAIWRSEVEYL
                     VTDKHVMWKRGRIRRTIDRGAISYARIHWHPSIPGLGDLELVRAVPTGALRRRLSLLL
                     PGGMGPDRLWAIIRGVPTSGGAGDGHRPLAQRLDEGERVLWSSRPISSWREWLPGGTR
                     QILTAAIGVLVGVAFVRELMSAIPAAKNVLAAGLPSRSAAFISLVMAVALTAILLGCI
                     AVGLGYVALVRPARLVQKTRYLITDRRVLIQRGHEELCLDRGRIVDVIDSPGQRGLHD
                     IFLVLDGPRARALAASGAFGERDVDGLVPVFHAVADAEEVSQILRLPGSPPPIHDAA"
BASE COUNT          352 a          634 c          699 g          275 t
ORIGIN      
        1 gagctcgcgg cgtgcgttct gtgcctcgcc gttgcgagcg atgcccgtgc cctcggtcat
       61 tccccgggcg tctgggagaa agccgccaat cccattctcg attcgaacga cgaaacgcat
      121 cgcgaggtcc aggggttgct catgcagatg atgcccacgc cgggcgacta ctgggaatcg
      181 gggcagggaa gggagcagct tcgaacggcg ctcgagaagc tcgacgaggc gcgcgccgac
      241 acggcgcccg acgttcgcct gcggttcgac ctgggtgagg tcgcccacaa tctccgagac
      301 tacgctcggt gtagccgggc gctgcagagc gcgctggccg aggcgcccga cgaccctcag
      361 gccacccacg cctatttcat gctgggaatc tgccacgcga agttgcagca gccggaaaag
      421 gaggtgacgt gttacgacga gttcctgcgc cgtgagacga acacacttgg tcgcgctcag
      481 gcgctctcca accgtgccga agcgcagatg ctgatgcacc gtcttccgcc cgcgatcgcc
      541 gactatcgcg catcgctggc gctccagccc gacatggcgc tcacgcattt tggtctcgcg
      601 gtggcgctcg atcgcggggg agatgcgccg ggggcgatgg tcgaggcgaa ggccgcggcg
      661 acgtacgatc cgctcgacca ggacctcacg cgcgccgacg ttttcttcat gcctccgtac
      721 gatctttcgt tctacgaggc cctcaacgca atggcgcgcg ctcaaatggt cgacgacccc
      781 gccgcgtccg ttctgtggtg ggagaccgcg gttgccaagt ggatggagta cgttgcgctg
      841 gcggcctcga gcgacccatg gatcgcgctg gccaaagcgc accaatcctc gtgcgagcga
      901 cagctcacgg cggccaagaa aaagctggct cgcccgctca agaccaaaaa aaagggctag
      961 gccgcgtcgt ggatgggcgg ggggctgccc ggtaggcgca ggatctggct gacctcttcg
     1021 gcatccgcaa ccgcgtggaa gacgggcacg aggccatcga cgtcgcgctc gccgaacgcg
     1081 ccactggcgg cgagagcgcg agcgcggggg ccatcgagca cgagaaagat gtcgtgcaaa
     1141 ccgcgctgac ccggactgtc gatgacgtcg acgatgcgtc cgcgatcgag gcacagctct
     1201 tcgtgacctc gctggatgag cacgcggcga tcggtaatga ggtagcgcgt cttctggacg
     1261 agccgggcgg gccgcacgag ggcgacgtag ccgagcccca cggcgatgca gccaagcaag
     1321 atcgcggtca acgccacggc catcaccagc gagatgaacg ccgccgatcg actggggagc
     1381 ccggcggcga gcacgttttt ggcggccgga atcgcggaca tgagctcgcg tacgaaggcc
     1441 acgcccacca gaacgccgat cgcggcggtg aggatctggc gcgtgccgcc ggggagccac
     1501 tcccgccagc tcgaaatggg gcgcgacgac cagagcacgc gttcaccttc gtcgaggcgt
     1561 tgcgccagcg gccgatgccc gtcgcccgcg ccgcccgagg tggggacacc gcgaatgatg
     1621 gcccacaggc ggtcgggccc catgccgccc ggcaagagga gcgaaaggcg acgccggagc
     1681 gcacccgtcg ggacggcccg gacgagctcg agatcgccca ggccggggat gctgggatgc
     1741 cagtggatgc gcgcatagct gatggcaccg cgatcgatgg tgcgacggat gcggccgcgc
     1801 ttccacatga cgtgcttgtc ggtgacgagg tactcgacct cggatcgcca gatggcaggc
     1861 ccatagcgca gggccagggc gagcgtggcg gaccagccgg cgaagaggat gaggccccct
     1921 gcgggcacgt ggagggcggt ggcgacgagg atggcgaagg
//
LOCUS       DASVII010000440         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_163506,
            whole genome shotgun sequence.
ACCESSION   DASVII010000440 DASVII010000000
VERSION     DASVII010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163506"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF309_05755"
     CDS             <1..234
                     /locus_tag="VF309_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEX7466126.1"
                     /translation="ATWIRPEEGIVLASGAGALGARLGEPIPLGATLVDRPALGTGEL
                     PREDALASLEGLLWRALILWLIVFALAAALQLA"
     gene            268..1668
                     /locus_tag="VF309_05760"
     CDS             268..1668
                     /locus_tag="VF309_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX7466127.1"
                     /translation="MELTFDRFYRYDDLTAILEGFAARNPDLFKVESLGKSHEGRDIW
                     VVTATRASKGAAADKPAFWVDGNIHAAELAASTACLYYLHTLERGYGTDADVTRLLDT
                     RTLYVCPRINPDGAEWALADKPKYIRSSTRPYPFDEEPVDGLNVEDVDGDGRILSMRI
                     RDPNGAYKAHAADPRLMTPREPAEAGGEYFRVIPEGTLANYDGVEIRINRDKQGIDLN
                     RNFPSGWRQEFQQLGAGPYPTSEPEVRAVVDFITRHANICGGVSFHTHSGVILRPFAS
                     EPDDNMPAEDLWLYKLFGKKSTELTGYPNISIFHDFKYHPKQVITGGFDWIYEHLGQF
                     FWTVEIWAPVKEAGVETYHFIEWYREHPAEDDLKLLAWSDKELAGEGYVDWYPFEHPQ
                     LGPVEIGGWNKLQVFRNPPKTISSSSRGATGSSRARATSTGIRSTIRSWAASSSADGT
                     SCRCSAIRRRNISSAR"
     gene            1626..>1918
                     /locus_tag="VF309_05765"
     CDS             1626..>1918
                     /locus_tag="VF309_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466128.1"
                     /translation="MFRNPPKKYLEREVKRFPGWLTWQALTLPKLELLAAEVESLGNE
                     TWRVRLAVQNTGYLPSYVTKRALERKMTRGVVYEIELPQGAALVAGKPRAEGA"
BASE COUNT          336 a          650 c          639 g          293 t
ORIGIN      
        1 gccacctgga tccggcccga agagggcatc gtgctcgcga gcggcgccgg ggcgctcggc
       61 gcgcggctgg gcgagccgat tcccctcggc gcgacgctcg tcgatcgccc cgcgctcggg
      121 acgggagagc tcccgcgcga ggacgcgctc gcgagcctcg agggcctgct gtggcgcgcg
      181 ctcatcctct ggctcatcgt cttcgccctg gcggccgcgt tgcagctcgc ctaggcttcc
      241 ccatacaacc ggaaagggca cgcatccttg gagctcacct tcgatcgttt ctaccgctac
      301 gacgacctca ccgcgatcct cgaggggttc gccgcgcgca atccggatct gttcaaggtc
      361 gaaagcctcg gcaagagcca cgaagggcgc gacatctggg tggtgaccgc gacccgggcg
      421 tcgaagggcg cggcggcgga caagcccgcc ttctgggtcg acggcaacat tcatgcggcg
      481 gagctcgcgg cctcgacggc gtgcctctac tacctgcaca cgctcgagcg gggctacggc
      541 accgatgccg acgtcacgag gctcctcgac acgcgcacgc tctacgtgtg cccgcgaatc
      601 aatcccgacg gcgcggaatg ggcgctcgcc gacaagccga agtacatccg ctcgtcgacg
      661 cgtccctatc cgttcgacga ggagccggtc gacgggttga acgtcgagga tgtcgacggc
      721 gacgggcgca tcctctcgat gcgcattcgc gaccccaacg gcgcctacaa ggcgcacgcc
      781 gccgatccgc gcctcatgac cccgcgcgag cccgccgagg cgggcggcga gtatttccgc
      841 gtcattcccg agggcacgct cgccaactac gacggcgtcg agatccgcat caatcgcgac
      901 aagcagggca tcgacctcaa tcgcaacttt ccctcgggat ggcgccagga gttccagcag
      961 ctcggcgcgg ggccctatcc gacctccgag ccggaagtgc gcgccgtggt ggatttcatc
     1021 acgcggcacg cgaacatctg cggcggcgtg agcttccaca cgcacagcgg cgtgatcctc
     1081 aggcccttcg cgagcgagcc cgacgacaac atgccggcgg aggacctgtg gctctacaag
     1141 ctcttcggca agaagtcgac ggagctcacg ggctatccga acatctcgat cttccacgac
     1201 ttcaagtacc acccgaagca ggtgatcacc ggcggcttcg actggatcta cgagcacctc
     1261 gggcagttct tctggaccgt ggagatctgg gcgccggtga aggaagcggg cgtggagacc
     1321 taccatttca tcgagtggta tcgcgagcat cccgccgaag acgacctgaa gttgctcgcg
     1381 tggagcgaca aggagctcgc gggcgagggc tacgtcgact ggtatccgtt cgagcatccg
     1441 cagctgggcc cggtcgagat cggcggatgg aacaagctgc aggtgttccg caacccgccg
     1501 aagacgatct caagctcctc gcgtggagcg acagggagct cgcgggcgag ggctacatcg
     1561 actggtatcc gttcgaccat ccgcagctgg gccgcgtcga gctcggcgga tggaacaagc
     1621 tgcaggtgtt ccgcaatccg ccgaagaaat atctcgagcg cgaggtgaag cgctttcccg
     1681 gctggctcac gtggcaggcg ctcacgctgc cgaagctcga gctgctcgca gccgaggtcg
     1741 aatccctggg caacgaaacg tggcgcgtgc ggctcgccgt gcagaacacg gggtacctcc
     1801 cgtcctatgt caccaagcgc gcgctcgagc gcaagatgac gcgcggcgtg gtctacgaga
     1861 tcgagcttcc gcagggcgcc gcgctcgtgg cggggaagcc gcgcgcggaa ggcgcgca
//
LOCUS       DASVII010000441         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_91538, whole
            genome shotgun sequence.
ACCESSION   DASVII010000441 DASVII010000000
VERSION     DASVII010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91538"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VF309_05770"
     CDS             <1..553
                     /locus_tag="VF309_05770"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX7466129.1"
                     /translation="VVDFWFGASLARGTARPEWFRKDVKFDAEIRRLFVALHASAALG
                     EREAWRSQPRSMLALVIVLDQFSRNLYRGDGRAFAQDAHALACSREALARGDDAGLLP
                     VERQFLYMPFEHSEDLADQDRAVELMRSLGAFEQTRGLEEWAERHRAVIRRFGRFPHR
                     NAALGRASTPQEIEFLKEPGSAF"
     gene            complement(554..685)
                     /locus_tag="VF309_05775"
     CDS             complement(554..685)
                     /locus_tag="VF309_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEX7466130.1"
                     /translation="MGRGDKRTFKGKVFKGSHGKTRLKNRANAKAKAAAIAATAKKK"
     gene            complement(730..>1915)
                     /locus_tag="VF309_05780"
     CDS             complement(730..>1915)
                     /locus_tag="VF309_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466131.1"
                     /translation="VDAPPGVLSQFHVAAAEDAHAQAEAWKRWSEDFTRDMRTSMGTL
                     FSGRMASGRIVKGAPYSAEVITETNQTLADGNVISRKTSGRVYRDGEGRSRQESGTAG
                     DMNVFINDPVASRHVMLYPGPKRAIVAPGSFGMARAGTHSKQVIRVDGTEEVRVEDGK
                     VFLNGKEVAGGGLVIKAKSGKEVKVENGKLTVDGKEVDSGEGTGSHVHVTREVVDGVP
                     REEVRVKVVRMGDDPLPPVPPIPPMPPELPGSPRAAVPPIPPVPPVPPLPPMPGLDSF
                     HFESTAGLGKGVTTSLGTKEFDGVKAEGTSTVWTIPAGRIGNRNPINVTKESWYAHDL
                     QVLVYARHNDPRTGESIYRLASIRRAEPAADLFKVPEGYTVQGRDRRGNAPEAKPASP
                     PG"
BASE COUNT          251 a          657 c          643 g          364 t
ORIGIN      
        1 cgtcgtggac ttctggttcg gggcgtcgct ggcgcggggg acggcgcgtc cggagtggtt
       61 ccggaaggac gtgaagttcg acgcggagat ccggcgtctc ttcgtggcgc tgcacgcgag
      121 cgccgcgctt ggcgagcgcg aggcctggcg ctcgcagccg cgctcgatgc tcgccctggt
      181 gatcgtcctc gaccagtttt cccgcaacct ctatcgaggg gatggacgcg ccttcgcgca
      241 ggacgcgcac gccctcgcgt gctcgcgcga agcgctcgcg aggggagacg acgcgggtct
      301 gctcccggtc gagcggcagt tcctgtacat gccgttcgag cacagcgagg atctcgccga
      361 ccaggaccgc gccgtggagc tgatgcgctc gctgggcgcg ttcgagcaga ctcgcgggct
      421 cgaggagtgg gccgagcgcc accgcgccgt catccggcgc ttcggcaggt tccctcaccg
      481 caacgccgcc ctgggccgcg cctcgacgcc gcaggaaatc gagttcctga aggaacccgg
      541 ctcggcgttc tgactacttc ttcttggccg tggccgcgat cgccgcggcc ttcgccttcg
      601 cgttcgcccg gttcttcagc cgcgtcttgc cgtggctgcc cttgaagacc ttgcccttga
      661 aagtgcgctt gtcgcccctg cccatggtgc cgttccttcg gtggataagg gtcgattata
      721 gaatgctcgt caacccggag ggctcgcagg cttcgcttcg ggtgcatttc cgcgccgatc
      781 gcgtccctgc accgtatagc cctcggggac cttgaagaga tccgccgcgg gctcggcgcg
      841 cctgatcgag gcgaggcggt agatcgattc tccggtgcgc ggatcgttgt ggcgcgcgta
      901 caccagcact tgcaggtcgt gcgcgtacca ggactccttc gtgacgttga tggggttgcg
      961 gttgccgatg cggcccgcgg gaatcgtcca gaccgtggac gttccctcgg ccttcacgcc
     1021 gtcgaattcc ttcgtgccca gcgacgtcgt cacgcccttg ccgagtcccg cggtgctctc
     1081 gaaatgaaac gagtcgagtc cgggcatggg cgggagcggt ggcacgggcg gcaccggggg
     1141 tattggcggc acggccgcgc gcggcgaccc agggagttcg gggggcatcg gcggaatggg
     1201 cggaaccggt gggagcgggt cgtcacccat ccgcaccacc ttcacgcgca cttcctcgcg
     1261 cggcacgccg tcgacgactt cgcgcgtgac atggacatgg gagccggtgc cctcgccgct
     1321 gtcgacttcc ttgccgtcga cggtgagctt gccgttctcg accttcacct ccttgccgct
     1381 cttcgccttg atgacgagcc ctccgcccgc gacttccttg ccgttgagga agaccttgcc
     1441 gtcctcgacg cgcacctcct ccgtgccgtc caccctgatc acctgcttgc tgtgagtgcc
     1501 ggcgcgggcc atgccgaacg agccgggggc cacgatggcg cgcttcggtc ccggatacag
     1561 catgacgtgc cggctcgcga cgggatcgtt gatgaagaca ttcatgtcgc ccgcggtgcc
     1621 cgactcctgg cgggatcgac cctcgccgtc gcgatacacg cgaccggacg tcttcctgct
     1681 gatgacgttc ccgtcggcga gcgtctggtt cgtttccgtg atcacctcgg cgctataggg
     1741 cgctcccttc acgatccttc ccgacgccat gcgcccgctg aagagggttc ccatcgaggt
     1801 tcgcatgtcg cgggtgaaat cctcggacca gcgtttccat gcttcggctt gcgcgtgcgc
     1861 atcctcggcc gccgcgacat ggaattgcga aagcacgccc ggaggcgcgt ccacg
//
LOCUS       DASVII010000442         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_163651,
            whole genome shotgun sequence.
ACCESSION   DASVII010000442 DASVII010000000
VERSION     DASVII010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163651"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1658)
                     /locus_tag="VF309_05785"
     CDS             complement(<1..>1658)
                     /locus_tag="VF309_05785"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466132.1"
                     /translation="RAFIAQYYDNVDPEDLADRAPEDLYGAAMAHLSFARVYFSGMPK
                     LRVYNPRPEEHGWSSPHTVIEIVNDDMPFLVDSVTMEVNRQGYTLHLFNHPLFATRRD
                     EEGHLQSFGPPGKDGRHESLIHVEVDREIDVAKLKELGNGILHVLADVRASFEDWDVM
                     RERMTGIVKELQAPPAFLRGEETDETRAFLEWAADHHFTFLGYRDYELASVEGEDQLR
                     IVPRSGLGVLREPKLGGVSQSFAELPAQLRALAREPRLLVLTKANARASVHRPGYLDY
                     IGVKRFDDAGKVIGERRFVGLYTSSAYHADPRQVPLLRRKVAKVIERAGFRQYSHMYK
                     ALLAILQAYPRDELFQIDEDTLLETALGILRLGDRRKTRAFIRRDLYGRFYSCLVFLP
                     RESYNTDVRVKIQDILKRHLQGTTAEFTVQLSDAALARIHLLVRANPKDAPAYDIHAI
                     EADLARATRRWEDDLKIALIEAFGEERATVLVRRYGSAFPIAYRDAITPRAAVRDVAF
                     IEKLDAEHPYAVNLYRPVEADERALRLRVFRIGASVPLSASLPV"
BASE COUNT          253 a          551 c          599 g          255 t
ORIGIN      
        1 gaccggcagg ctcgcggaaa gcggcaccga cgcgccaatg cggaagacgc gaaggcgcag
       61 cgcgcgctcg tcggcctcca ccgggcgata caggttcacg gcatagggat gctcggcgtc
      121 gagcttctcg atgaaggcga cgtcgcgcac cgcggcgcgc ggcgtgatgg cgtcgcgata
      181 ggcgatgggg aaggcgctcc cgtagcgccg cacgaggacg gtcgcgcgct cctcgccgaa
      241 ggcctcgatg agggcgatct tgaggtcgtc ctcccagcgc cgcgtcgcgc gcgcgaggtc
      301 cgcctcgatg gcgtggatat cgtaggccgg cgcatccttc gggttcgccc gcacgaggag
      361 gtggatgcgc gcgagcgcgg cgtccgacag ctgcaccgtg aactccgccg tcgtgccctg
      421 caggtggcgc ttcaggatgt cctggatctt cacgcggaca tcggtgttgt agctctcgcg
      481 gggcaggaac acgaggcacg agtagaaacg cccgtagagg tcgcggcgga tgaaggcgcg
      541 ggtcttgcgc cggtcgccga ggcgcaggat gccgagcgcg gtctcgagga gcgtgtcctc
      601 gtcgatctgg aagagctcgt cgcgcggata ggcctgcagg atcgccagca gcgccttgta
      661 catgtggctg tactgccgga agcccgcgcg ctcgatcacc ttcgcgacct tgcggcgcag
      721 gagcggcacc tgccgcgggt ccgcgtggta ggccgaggac gtatagaggc cgacgaagcg
      781 gcgctcgccg atcaccttgc ccgcgtcgtc gaaacgcttg acgccgatgt agtccaggta
      841 gccggggcgg tggacgctcg cccgcgcgtt cgccttggtg agcaccagca ggcgcggctc
      901 gcgcgcgagc gcgcgaagct gcgccggaag ctcggcgaag ctctgcgaaa cgccgcccag
      961 cttcggctcg cgcagcacgc ccagccccga gcgcggcacg attcgcagct ggtcctcgcc
     1021 ctcgaccgag gcgagctcgt agtcgcggta gccgaggaac gtgaagtgat ggtccgcggc
     1081 ccattcgagg aaggcgcgcg tctcgtcggt ctcctcgccc cgcaggaagg cgggcggcgc
     1141 ctgcagctcc ttcacgatgc cggtcatgcg ctcgcgcatc acgtcccagt cctcgaacga
     1201 cgcgcgcacg tcggcgagga cgtgcaggat gccgttgccc agctccttca gcttcgcgac
     1261 gtcgatctcg cggtccacct cgacgtggat gagcgactcg tgccggccgt ccttccccgg
     1321 cggcccgaag gattgcaggt gaccctcctc gtcgcgccgc gtggcgaaca gcgggtggtt
     1381 gaacaggtgc agcgtgtagc cctgccggtt cacctccatc gtcaccgagt cgaccaggaa
     1441 aggcatgtcg tcgttcacga tctcgatcac ggtgtgcggg ctcgaccagc cgtgctcctc
     1501 gggacgcggg ttgtacaccc gcagcttcgg catcccggag aagtacaccc gcgcgaacga
     1561 gaggtgcgcc atggccgcgc cgtagagatc ctcgggggcg cgatcggcga ggtcctcggg
     1621 atcgacgttg tcgtagtact gcgcgatgaa ggcgcgcg
//
LOCUS       DASVII010000443         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_487760,
            whole genome shotgun sequence.
ACCESSION   DASVII010000443 DASVII010000000
VERSION     DASVII010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487760"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1205
                     /locus_tag="VF309_05790"
     CDS             96..1205
                     /locus_tag="VF309_05790"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX7466133.1"
                     /translation="MRIHKLAAIGGDGIGPEVIAAGLDVLETLATSDGGFRFEVENFA
                     WSSDFYTKHGHYIPPGGLEKLKEFDAILFGAVGSREVPDHVSLWGLRLPICQGFDQYA
                     NVRPTRVLPGVKSPLANGHEIDWVVVRENSEGEYAGMGGRSHRGLPIEVGTEVAVFTR
                     AGVERIHRFAFGLARTRPRKHLTLVTKSNAQRFGMVMWDEIFFEVAKDYPDVKVDREL
                     VDAMTTRMVLKPATLDVMVATNLHADILSDLAAALAGSLGIAPTANLNPERKFPSMFE
                     PIHGSAFDITGKGVANPIATFWTASMMLDHLGEHDASRRLMNAIERVTANGVHTPDLG
                     GTAKTIDVTKAVRKAVLDAAKSGTQQLEPLKQTTT"
     gene            1216..>1848
                     /locus_tag="VF309_05795"
     CDS             1216..>1848
                     /locus_tag="VF309_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX7466134.1"
                     /translation="MALKRPSWGAGTVVVLEHDSKILRDNPLGDPHLRKLAVWLPPQY
                     DEGATRGAGRRFPAFYDLVGFMGSGLAHVGWKNFGENVAERAARLVHEKKMGPAIIVF
                     PDCFTALGGNQYVNSSAIGRYADYVTQEIVPFVDREFRTLASREHRGCFGKSSGGYGS
                     IIHGMKYPKTWGAIADHSGDAYFEFVYWHDWPNTLNELSKYRARPAKPGAF"
BASE COUNT          326 a          620 c          614 g          288 t
ORIGIN      
        1 agcgacatcg tcttgcgagc gccagcgata acgtcctgaa agcgccagcg gacaagctgc
       61 cttacaattt gcccatagag agcgaggagc acgacatgcg catccacaag ttggccgcca
      121 tcggcggcga tggaatcggt cccgaggtga tcgccgcggg cctcgacgtg ctcgagacgc
      181 tcgcgacctc cgacggcggt ttccgcttcg aggtggagaa tttcgcgtgg agctcggact
      241 tctacacgaa gcacgggcac tacatcccgc cgggcggcct cgagaagctg aaggaattcg
      301 atgcgatcct cttcggagcg gtcggaagcc gggaagtgcc cgaccacgtg tcgctgtggg
      361 ggctgcgcct gccgatctgc cagggcttcg accagtacgc gaacgtgcgg cccacgcgcg
      421 tcctcccggg cgtgaagagc ccgctcgcca acgggcacga gatcgactgg gtcgtggtcc
      481 gcgagaactc cgagggcgag tacgcgggca tgggcgggcg ctcgcaccgc ggccttccga
      541 tcgaggtcgg gaccgaggtc gcggtgttca cgcgcgcggg cgtcgagcgc atccatcgct
      601 tcgccttcgg cctggcgcgc acgcggcccc gcaagcacct cacgctcgtg accaagtcga
      661 acgcccagcg cttcggcatg gtgatgtggg acgagatctt cttcgaggtc gcgaaggact
      721 atcccgacgt gaaggtcgac cgcgagctcg tcgacgcgat gacgacgcgc atggtgctga
      781 agcccgcgac gctcgacgtg atggtcgcga ccaacctgca cgccgatatc ctctcggatc
      841 tcgcggcggc gctcgccgga agcctcggca tcgcgcccac ggcgaacctg aacccggagc
      901 gcaagttccc gtcgatgttc gagccgatcc acggctcggc cttcgacatc accggcaagg
      961 gcgtggccaa tccgatcgcg accttctgga cggcttcgat gatgctcgat cacctgggcg
     1021 agcacgacgc ctcgcgccgg ctcatgaacg cgatcgagcg cgtcacggcg aacggcgtgc
     1081 atacgcccga cctcggcggc acggcgaaga cgatcgacgt gaccaaggcg gtgcgcaagg
     1141 ccgtcctcga tgcggcgaag tccgggacgc agcagctcga acccctgaag caaacgacga
     1201 cttgaaggag tttcaatggc gttgaaacgg ccctcgtggg gcgcggggac ggtcgtcgtc
     1261 ctcgagcacg actcgaagat cctgcgcgac aacccgctcg gcgatccgca cctgcgcaag
     1321 ctcgcggtgt ggctcccgcc ccagtacgac gagggcgcca cgcgcggcgc gggaaggcgc
     1381 ttcccggcgt tctacgacct cgtcggcttc atgggctcgg ggctcgcgca cgtgggctgg
     1441 aagaacttcg gcgagaacgt cgccgagcgc gccgcgcgcc tggtccacga gaagaagatg
     1501 ggcccggcga tcatcgtctt ccccgattgc ttcaccgcgt tgggcggcaa ccagtacgtg
     1561 aactcgtcag cgatcggccg ttacgcggac tacgtcaccc aggagatcgt gcccttcgtg
     1621 gaccgcgagt tccgcacgct cgcctcgcgc gagcatcgcg gctgcttcgg caagtcgtcc
     1681 ggcggatacg gctcgatcat ccacggcatg aaatatccga agacgtgggg cgcgatcgcg
     1741 gaccattccg gtgacgccta tttcgagttc gtctactggc acgactggcc gaacacgctg
     1801 aacgagctct cgaaatatcg cgcccggccc gccaaacccg gcgcgttc
//
LOCUS       DASVII010000444         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_199843,
            whole genome shotgun sequence.
ACCESSION   DASVII010000444 DASVII010000000
VERSION     DASVII010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199843"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VF309_05800"
     CDS             <1..1304
                     /locus_tag="VF309_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7466135.1"
                     /translation="DPAKLAAWLQAQGVGIVHAHSVDRAARRRASWAAQALGVAVVVT
                     LHDILFLRREGFEPGASRAPDSQWLAETAPFLRSAAAVIAPSHFVANLARGNVAGLEV
                     AVIPNGSPPRDPGVRTFEARAEFSRRRPRHVAAILGAVGPHKGSAIVNDLSRALEGTD
                     IAIVVIGYLDMQIVPGWRGGHVFVHGAYDDDAVAPLLAAYAAELALFPNLVPESFSYA
                     LSDAWDAGVPALVPPEGALAERVTRHGGGWLLPAAFDATAVAAALRHFLSAEGAGELA
                     RVKSRLALPDPGRVPTLDDMTRSLDALYARFAIDPAATLDPQSAPVRDLLAKNLDGAL
                     FRQELVRLADEMAQMKAALESTLEFERRQAGDFRNEAREWIAKLEGDVASLQAQVAVE
                     VEARRALGQEIVQLRIHKAAFDLLPDVIRRMLLKKIVNARS"
     gene            1291..>1605
                     /locus_tag="VF309_05805"
     CDS             1291..>1605
                     /locus_tag="VF309_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466136.1"
                     /translation="MREVDISIVTYQPDFPLLAQLLASLSEPTGDEVARNLFLQDNSP
                     DPEVAVRLVAMPELQPGAAFARVDVKISGANLGYGRGHNANAARGSAALVLLLNQDCV
                     LEP"
BASE COUNT          224 a          602 c          544 g          235 t
ORIGIN      
        1 gagatcccgc gaagctcgcc gcgtggctgc aggcgcaagg agtcggcatc gttcacgcgc
       61 actcggtcga tcgcgccgcg cgccgccgcg catcgtgggc ggcgcaagcg ctcggcgtcg
      121 cggtcgtggt caccttgcac gacatcctct tcctgcgccg cgagggcttc gagcccggcg
      181 cctcacgcgc ccccgattcc caatggctcg cggagacggc acccttcctg cggagcgcgg
      241 ccgccgtgat cgcgccgtcg catttcgtcg cgaatctcgc ccgaggcaat gtcgcgggcc
      301 tcgaggtcgc cgtgattccc aatggctcgc cgccgcggga tcccggcgtt cgcaccttcg
      361 aggcgcgggc cgaattctcg cggcgccgcc cgcggcacgt cgccgcgatc ctcggcgccg
      421 tcggcccgca caagggcagc gccatcgtga acgacctttc gcgcgcgctc gagggcaccg
      481 acatcgcgat cgtcgtcatc ggctacctcg acatgcagat cgtccccggg tggcgcggcg
      541 gccacgtctt cgtgcacggc gcctacgacg atgacgccgt cgcgccgctc ctcgcggcgt
      601 acgcagccga gctcgcgctc ttccccaacc tcgtcccgga aagcttcagc tatgcgctct
      661 ccgacgcgtg ggacgcgggc gtccccgcgc tcgtgccgcc cgagggggcg ctcgccgagc
      721 gcgtgacgcg ccacggcggc ggatggctcc tgcccgcggc gttcgacgcg accgcggtgg
      781 ccgcggcatt gcgtcatttc ctgtcggccg aaggggccgg ggagctcgcg cgagtaaaat
      841 cgcggctcgc gcttcccgac ccggggcgcg tcccgactct cgacgacatg acacgctccc
      901 tcgacgccct ctacgcgcgc ttcgcgatcg acccggccgc gacgctcgac ccgcagtcgg
      961 cgccggtgcg cgacctcctc gcgaagaacc tcgacggcgc gctcttccgc caggagctgg
     1021 tgcgcctcgc cgacgagatg gcgcaaatga aggccgcgct cgagtcgacg ctcgaattcg
     1081 agcggcggca ggcgggcgat ttcaggaacg aggcgcgcga atggatcgcg aagctcgagg
     1141 gcgacgtggc gagcctgcag gcgcaggtcg cggtcgaggt ggaggcgcgc cgcgcgctcg
     1201 gccaggagat cgtgcagctg cgcatccaca aggcggcgtt cgacctcctg cccgacgtga
     1261 ttcgcaggat gctattgaag aagatcgtca atgcgcgaag ttgacatcag catcgtcacc
     1321 taccaacccg acttcccgct tctcgcgcaa ctcctcgcga gcctctcgga acccaccggc
     1381 gacgaggtcg cgcgcaacct cttcctccag gacaacagcc ccgatcccga ggtcgcggtg
     1441 cgcctggtgg cgatgcccga gctgcagccg ggagcggcgt tcgcgcgcgt cgacgtcaag
     1501 atctcgggcg cgaacctcgg gtacggccgc ggccacaacg ccaatgccgc gcgcgggtcg
     1561 gcggccctcg tcctgctgct gaaccaggac tgcgtgctcg agccc
//
LOCUS       DASVII010000445         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_128467,
            whole genome shotgun sequence.
ACCESSION   DASVII010000445 DASVII010000000
VERSION     DASVII010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128467"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VF309_05810"
     CDS             <1..233
                     /locus_tag="VF309_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466137.1"
                     /translation="HALAVSIEDDGPGVPAELAERIFYPLVSGREGGTGLGLSLAQSF
                     VSQHHGLIEFESVPGHTRFTILLPVRSRNDTQ"
     gene            251..1550
                     /gene="ntrC"
                     /locus_tag="VF309_05815"
                     /pseudo
     CDS             251..1550
                     /gene="ntrC"
                     /locus_tag="VF309_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880663.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
BASE COUNT          266 a          532 c          522 g          230 t
ORIGIN      
        1 gccatgccct cgcggtttca atcgaggacg acggtcccgg agttcccgcg gagctcgccg
       61 agcggatctt ctatcccctg gtgagcgggc gcgagggcgg cacgggcctg ggactatcgc
      121 tcgcgcagag tttcgtgagc cagcatcatg gcctcatcga attcgaaagc gtgccgggcc
      181 acacgcgatt caccatcctg ctgccggtgc gttcgcgcaa cgacacgcaa tgaacgctcg
      241 cgaggacgcc atgaaccccg tctggataat cgacgacgac cgctcgatcc gatgggtgtt
      301 cgagaaggcg ctcgagcgcg agggcattgc gtaccgcacg ttcagcgcgg cgcaggacgc
      361 catcgacaag ctgccggaag agacgccgca ggtcgtggtg agcgacatcc gcatgcccgg
      421 catcaccggg ctcgagttca tgcagcagct gaaggcaaag ctgccctcca cgccggtcat
      481 catcatgacc gcgtattccg acctcgagag cgccgtcgcg gccttccagg gcggggcgtt
      541 cgagtacctc cccaagccgt tcgacgtcga ccacgcggtc gagctcgtgc ggcgcgcgct
      601 cgacgagagc cgccgccagg tccagccgcc gcccgaggac gccgaggcgc ccgagatcct
      661 cgggcaggcg cccgcgatgc aggaggtatt tcgcgccatc ggaaggcttt cccaatcgca
      721 cgcgacggtg ctcatcaccg gcgaataggg caccggaaag gagctggtgg cgcgggctct
      781 gcatcggcac agcccgcgtg cgtcgaaagc cttcatcgcg atcaacaccg ccgcgatccc
      841 caaggatctt ctcgaaagcg agctcttcgg gcacgagcgc ggcgcgttca cgggcgccca
      901 gacgatgcgc cgcggaaggt tcgagcaggc cgagggcggc tcgctctttc tcgacgagat
      961 cggcgacatg ccccaggacc tgcagacgcg gttgctgcgc gtgctttccg acgggcagtt
     1021 ctatcgcgtc gggggccaca cgccgctcgc cgcgaacgtt cgcgtcatcg ccgccacgca
     1081 ccaggagctc gagtcgcggg tgaagcaggg actgttccgc gaggacctct accaccgcct
     1141 caacgtgatt cgcctgcggc tgccgcccct gagggagcgg cgcgaggaca tcgccatgct
     1201 cgcgcggcat ttcctctcga agagcgccca cgacctcggc gtagagggca agcgcctcac
     1261 cgacggcgcc gtgcagtttt tcaccgcgca ggactggccc ggcaacgtgc gccagctgga
     1321 aaacgtgtgc cactgggtca cggtgatgga gccgggacag gtcgtcgacg tgaaggatct
     1381 ccccgctgag gtgcgcggcg aaagcgccgc ggctgcggcg ggcgatagcg actggcggcg
     1441 cctcctcgag cgcgaggtcg cgagccgcct ggcgcgcggc gaatcggaca tcgccgatcc
     1501 gctcacgcgc gatttcgaga ccgcgctcat cgttaaggcc ctgcagcaca
//
LOCUS       DASVII010000446         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_92078, whole
            genome shotgun sequence.
ACCESSION   DASVII010000446 DASVII010000000
VERSION     DASVII010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92078"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VF309_05820"
     CDS             <1..382
                     /locus_tag="VF309_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7466138.1"
                     /translation="IEPREPRSLEQGEYLQTPAQLERFESFSHCINCMLCYAACPQYG
                     LDSSFLGPAATALLHRYNSDSRDGAQAKRMELVNSEEGVWACTAVGYCSEVCPKAVDP
                     ANAVNQNKTASAVSWFARFLPGRG"
     gene            390..782
                     /locus_tag="VF309_05825"
     CDS             390..782
                     /locus_tag="VF309_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466139.1"
                     /translation="MRRTFHRSMDGWWRRDPFFIRYMAREATAPFVAAYAIVLLVGIV
                     SLARGADAYEEWLEALASPGWIVAHAILVAIFAYHTYTWFQIMPKTMPPVVVGGRRLA
                     PEAITFAGIAAAVLASLALWILAAYGAP"
     gene            779..1135
                     /gene="frdD"
                     /locus_tag="VF309_05830"
     CDS             779..1135
                     /gene="frdD"
                     /locus_tag="VF309_05830"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEX7466140.1"
                     /translation="MKRSNQPFFWLLFGAGGMLSALIGAMLVFITGVAAPLGLGVHAD
                     LMSHANAIAFARGIPGKLFLLVVVSLFLWHAAHRIYHSLHDLGIPPGPLARAACYGTA
                     LAGTLVAFAALLVIGF"
     gene            complement(1161..1619)
                     /locus_tag="VF309_05835"
     CDS             complement(1161..1619)
                     /locus_tag="VF309_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7466141.1"
                     /translation="MGDMIKHILVPIDDSPLSIKAAKYAASLARSSGARITALHVIPP
                     FRVMAGIDGVIADPELYSPVEYRKSTERFAWKLLAKGEAVAKASRVKCEAVFVTDGEP
                     WQAIIKAAKKRRCDLVVMASHGRRGIAAIVLGSETTKVLTHSKVPVLVVR"
BASE COUNT          259 a          614 c          597 g          320 t
ORIGIN      
        1 gatcgagccg cgcgagccgc gcagtctcga acagggcgag tatctgcaga ctcccgcgca
       61 gctcgagcgc ttcgagtcct tcagccattg catcaactgc atgctgtgct acgcggcctg
      121 cccgcaatac gggctcgact cgagcttcct cgggccggcg gcgacggcgc tgctgcatcg
      181 ctacaacagc gactcgcgcg acggcgcgca ggccaagcgc atggagcttg tcaattcgga
      241 ggaaggcgtg tgggcctgca cggcggtagg ctactgctcc gaggtctgtc ccaaggccgt
      301 ggatcccgcg aacgcggtga accagaacaa gacggcgagc gcggtcagct ggttcgcgcg
      361 ttttctgccg gggcggggct gacatggcga tgcggcggac attccacagg tccatggacg
      421 gatggtggcg gcgcgatccg ttcttcatcc gctacatggc gcgcgaggcg acggcgccct
      481 tcgtggccgc gtatgcgatc gttctcctcg tgggaatcgt gtcgctcgca cgcggcgccg
      541 acgcctacga ggagtggctc gaagcgctgg cgagccccgg ctggatcgtc gcgcacgcga
      601 tcctcgtcgc gatcttcgcc taccacacgt acacatggtt ccagatcatg cccaagacga
      661 tgccgcccgt cgtcgtgggg ggccgcaggc tcgcgcccga ggcgatcacc ttcgcgggaa
      721 tcgcggcggc cgtgctcgcc tcgctcgcgc tgtggatcct cgcagcctac ggcgcgccat
      781 gaagcgctcc aaccaaccct tcttctggct gctcttcgga gcgggcggga tgctgtcggc
      841 gctgatcggg gcgatgctcg tgttcatcac cggggtggcc gcgccgctcg ggctcggcgt
      901 gcatgcggac ttgatgagcc acgccaacgc catcgcgttc gcccgcggca ttccggggaa
      961 gctctttctc ctggtcgtcg tgtcgctttt cctgtggcac gcggcgcacc gcatctacca
     1021 cagcctccac gacctcggaa ttcctcccgg gccgctcgcg cgggcggcgt gctatggcac
     1081 ggcgctcgcc ggcacgctgg tggcgttcgc cgcgcttctc gtgatcggct tctgacgcgc
     1141 ggcgcgccgc gcgacccgct tcaccggacg acgagcaccg gcaccttcga gtgcgtgagg
     1201 accttcgtcg tctcgctgcc gagcacgatc gcggcgatgc cgcggcggcc gtgggatgcc
     1261 atcaccacga ggtcgcagcg ccgcttcttc gcggccttga tgatcgcctg ccacggctcg
     1321 ccgtcggtca cgaagaccgc ctcgcacttc acgcgggaag cctttgcgac cgcctcgccc
     1381 ttcgcgagga gcttccacgc gaagcgctcg gtcgacttgc ggtactcgac gggcgaatag
     1441 agctccggat cggctatgac gccgtcgatg ccagccatga ccctgaacgg cgggatcacg
     1501 tgcagcgccg tgatgcgcgc gccggaagag cgcgcgaggc tcgcagcgta tttcgcggcc
     1561 ttgatcgaca acggcgagtc gtcgatggga acgaggatgt gcttgatcat gtcgcccatg
     1621 ttagctgaat tgcgttcagg tttccccggc ttcgacagcc gcgagcggtt tggattgcgc
     1681 cgccgcgacg accggcagct ccacgaagaa tgtcgtgccc agtcccggcg cggactcgaa
     1741 tccgatgtct cctcccagac gctcgacgat gctcttggcg atgctcagcc
//
LOCUS       DASVII010000447         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_667934,
            whole genome shotgun sequence.
ACCESSION   DASVII010000447 DASVII010000000
VERSION     DASVII010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667934"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1130)
                     /locus_tag="VF309_05840"
     CDS             complement(132..1130)
                     /locus_tag="VF309_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7466142.1"
                     /translation="MGITLATLVCLVLLALAFDFMNGLHDAANSIATVVSTGVMKPHH
                     AVLFAAFFNFVAIFVFHLKVAATVGKGIVSPEVIDHYVIFGALVGAISWNGITWFFGL
                     PSSSSHALIGGLIGAAVAKVGFSSLVADGIWKTVLFIFLSPLLGFVLGGLLLVAVSWI
                     FRKSSPSRVDRWFRRLQLVSASAYSLGHGGNDAQKTIGIIWALLIASSVPGAKESAPA
                     WVIVSCYVAIGLGTFFGGWRIVKTMGQRITKLKPVHGFCAETGGAITLFLATALGIPV
                     STTHTITGAIVGVGSTRRVSAVRWGVAGNIVWAWIFTIPASAFIAAVSWWLGTQVL"
     gene            complement(1130..1759)
                     /locus_tag="VF309_05845"
     CDS             complement(1130..1759)
                     /locus_tag="VF309_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX7466143.1"
                     /translation="MFGRFLPREGRFFDLFNAHAVQIVRAGNELSALMQNYENREEHA
                     RIIDDAEHAADRITAEAIRLLHKSFITPIDRDHILQLVNAMDDICDLIQDTAETLTLY
                     DIRAVTPEAARLADISMKCCERVKDVVDLLGTREGKFEAILKTCDEIDRLESDADRVM
                     RSAVSRLFREEQDVRELIKMKAIYELLEAITDRCLDVANLAEGISLENS"
     gene            2120..>2454
                     /locus_tag="VF309_05850"
     CDS             2120..>2454
                     /locus_tag="VF309_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466144.1"
                     /translation="MSTLFAQLHPSRWLTGLWLNSDFRKLWGSLTIVHFGGQVTFLAL
                     PLTAALMLNASPFEVGILPALEALPYPIFGLLAGVLVDRARKLPVIIACDVGRGLALL
                     AIPAFAWLGV"
BASE COUNT          426 a          792 c          823 g          413 t
ORIGIN      
        1 cgccgcgtgg gcgcaggaaa tggcggccgc aatggccagg aatggaatgg cacgacgatt
       61 cacggcggat ctcccaagtt ggtattcgtc gcaggatacc cggattcccg gcggggcaac
      121 cgtccaagaa cctataggac ctgggtcccc agccaccagc tcaccgccgc gatgaacgcc
      181 gaggccggga tcgtgaagat ccacgcccac acgatgttcc ccgccacgcc ccagcgaacc
      241 gcggacaccc tccgcgtgga gcccactccg acgatggccc cggtgatggt gtgcgtggtg
      301 gagacgggaa tgccgagggc cgtcgcgagg aagagcgtga tcgctccgcc cgtctcggcg
      361 cagaagccgt gcacgggctt cagcttcgtg attcgctggc ccatggtctt cacgatgcgc
      421 catccgccga agaaggtgcc caggccgatc gcgacgtagc acgagacgat cacccaggcg
      481 ggagcgctct ccttcgcgcc gggaacgctg gaagcgatga ggagcgccca gatgattccg
      541 atcgtcttct gggcgtcgtt gccgccgtgc ccgaggctgt acgcggaggc cgaaaccagc
      601 tgcagcctgc ggaaccatcg atcgacgcgc gaggggctcg acttgcggaa gatccacgat
      661 acggcgacga ggaggagccc cccgagcacg aatcccagga gcggcgaaag gaagatgaag
      721 agcaccgtct tccagatgcc gtcggccacg agcgagctga agccgacctt cgcgaccgcc
      781 gcgccgatga gcccgccgat cagggcgtgg gacgacgagg agggcaagcc gaagaaccac
      841 gtgatgccgt tccacgagat cgcgcccacg agggcgccga agatcacgta atggtcgatc
      901 acctcgggcg agacgattcc cttgcccacg gtcgccgcga ccttgaggtg gaagacgaag
      961 atcgcgacga agttgaagaa cgccgcgaag aggaccgcgt ggtgcggctt catgacgccg
     1021 gtggagacga cggtcgcgat ggagttcgcc gcgtcgtgca ggccgttcat gaagtcgaac
     1081 gcgagcgcca ggaggaccag gcacacgagc gtcgcgagcg tgatgcccat caggaattct
     1141 ctagcgagat gccttccgcc agattcgcca cgtcgaggca gcggtcggtg atcgcctcga
     1201 gcagctcgta gatcgccttc atcttgatca gctcgcgcac gtcctgctcc tcgcggaaga
     1261 ggcgcgaaac cgccgagcgc atcacgcggt cggcgtcgga ctcgaggcgg tcgatctcgt
     1321 cgcaggtctt caggatcgcc tcgaacttgc cctcgcgcgt gcccaggaga tcgacgacgt
     1381 ccttcacccg ctcgcagcat ttcatgctga tgtcggcgag gcgcgcggcc tccggcgtga
     1441 ccgcgcggat gtcgtagagc gtgagcgtct cggccgtgtc ctggatcagg tcgcagatgt
     1501 cgtccatcgc gttcacgagc tgcaggatgt ggtcgcggtc gatcggcgtg atgaagctct
     1561 tgtgcaggag ccggatcgct tcggccgtga tgcggtcggc ggcatgctcg gcatcgtcga
     1621 tgatgcgcgc gtgctcctcg cgattctcgt agttctgcat gagcgccgag agctcgttgc
     1681 cggcgcggac gatctggacg gcgtgggcgt tgaacaggtc gaagaagcga ccttcccggg
     1741 ggaggaaccg gccgaacatg ggagcacctg gcgtttaagt tggcgcgagt gtatccgaaa
     1801 aatacgcgag cgaaagcgcg gcttccgggc cggggatcgg ttcttcccgc tttgctataa
     1861 tgcgcgctcg aagaaccttt tttggatcca gcccaatgat tcgcagcgcc gcacccatcg
     1921 attcccgaga cctcgtctcg gcggtcgcgc gcgctcgttt cggggcctcg ctgccgtccg
     1981 gcggcgacga ctgatcccgc ggccgatccg ccgccggatt caaggggcgg agaggatcgg
     2041 caacattccc gatagctttt ccgcccgcgc ttaaccgcgc gacggcgcac cttccgtcgc
     2101 gcattggcag aagaagaaca tgtcgacatt gttcgcgcag ttgcatccga gccgctggct
     2161 cacgggcctt tggctcaact ccgacttccg caagctgtgg ggatcgctca cgatcgtcca
     2221 cttcggcggc caggtcacgt tcctcgcgct gccgctcacc gcggccctga tgctcaacgc
     2281 ctcgcccttc gaggtcggga ttctgccggc gctcgaggcg ctgccctatc ccatcttcgg
     2341 cctgctcgcg ggcgtgctgg tggaccgcgc gcgcaagctt ccggtgatca tcgcgtgcga
     2401 cgtggggcgg ggactcgcgc tcctggcgat tcccgcgttc gcctggctcg gagt
//
LOCUS       DASVII010000448         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_128611,
            whole genome shotgun sequence.
ACCESSION   DASVII010000448 DASVII010000000
VERSION     DASVII010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128611"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1166)
                     /gene="gspL"
                     /locus_tag="VF309_05855"
     CDS             complement(54..1166)
                     /gene="gspL"
                     /locus_tag="VF309_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEX7466145.1"
                     /translation="MKLRIFLPSVERPDASTRFAWMLFDERRALLREGTSTLDEIPRS
                     DDAEAVLPSTRVLFARLTLPKVSAATLRELLPYAVEDRLLADPSHIHAVAGKARGQGE
                     TPVAVIDREWLRAMLDALARSGIHPTCAWPESCLLAGGRDDWNLVWGERRGMLVDDEG
                     VSATFDHDASGSLPLALRLAIDEASARGQKPASLRVHTEGGSRLPELARWSAEAGVAF
                     APGTQWEILSRGEPSNECIDLMQGDFARRRAGPSRLPRAAVALAAAIAVLQLAFVAVD
                     AARLQHERAGLEARREALFRGAFPEAKVVVDPDLQMRRNLAELKRSRGLASQDDFLGR
                     LSRAALESPQPAKSIEYANGKLVIRRGEASVAEASR"
     gene            complement(1183..>1546)
                     /locus_tag="VF309_05860"
     CDS             complement(1183..>1546)
                     /locus_tag="VF309_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466146.1"
                     /translation="RGFDAATVAKLRPFVTALPERTSVNVNTASEALLDVVLPLPKES
                     VALFVKQRVARPFVSTQALAQWAKASLPEDLVNVKSSYFSVLVRVAQDDVELATDALL
                     SRPGQSGPPVIIWRRSRY"
BASE COUNT          235 a          533 c          570 g          208 t
ORIGIN      
        1 gcggacgatg ctgcgctcgc gcgccgagcg cgagcgccag agaatggcga ggctcatcgc
       61 gaggcctccg cgaccgacgc ttcgccgcga cggatgacga gcttgccgtt ggcatattcg
      121 atcgacttcg ccggctgcgg cgactccagc gccgcacgcg acaggcgtcc gaggaaatcg
      181 tcctgggacg cgaggcctcg cgagcgcttc agctccgcga ggttgcggcg catctgcagg
      241 tccggatcga ccaccacctt ggcctcaggg aacgcgccgc ggaagagcgc ttcgcggcgc
      301 gcttcgaggc ccgcgcgctc gtgctggagg cgcgccgcgt cgaccgcgac gaaggcgagc
      361 tgcaggacgg cgatcgccgc cgcgagcgcg accgccgccc gcggcaagcg cgaaggaccg
      421 gcgcggcgac gggcgaaatc gccctgcatg aggtcgatgc attcgttcga aggctcgccg
      481 cgggacagga tctcccattg cgttcccggc gcgaacgcca cgcccgcctc ggcgctccag
      541 cgcgcgagct ccgggaggcg cgagccgccc tcggtgtgca cgcgaagcga cgcgggcttc
      601 tgcccgcggg cggacgcctc gtcgatcgcg aggcgaagcg ccagcggcag gcttcccgac
      661 gcgtcgtggt cgaaggtggc gctgacgcct tcgtcgtcga cgagcatgcc gcgccgctcg
      721 ccccagacga ggttccagtc gtcgcggcct cccgcgagaa ggcagctctc gggccatgcg
      781 caggtcgggt ggatccccga tcgcgcgagc gcgtcgagca tcgcgcgcag ccactcgcga
      841 tcgatcaccg cgacgggcgt ctcgccctgg ccgcgcgctt tccccgcgac ggcgtggatg
      901 tgcgagggat cggcgagcaa ccggtcctct accgcatagg gcagcagctc gcgcagggtc
      961 gcggcgctca ccttgggaag cgtcaggcgc gcgaacagca cgcgcgtgga aggcagcacg
     1021 gcttcagcgt cgtcgcttcg cgggatctcg tcgagcgtcg aggtgccttc gcgaagcagc
     1081 gcgcggcgct cgtcaaaaag catccacgcg aagcgggtgg atgcgtcggg gcgttcgacg
     1141 gagggcagga agatgcgcag cttcatcgcg gggatgccgt gcctaataac gcgagcggcg
     1201 ccagattata acgggcgggc cgctctgccc cgggcgcgag aggagcgcgt cggtcgcgag
     1261 ctcgacgtcg tcctgggcga cgcgcaccag caccgagaag taggagctct tcacgttgac
     1321 caggtcctcc gggaggctcg ccttggccca ttgcgcgagg gcttgcgtcg agacgaaggg
     1381 cctggcgacg cgctgcttca cgaacagcgc gacgctttcc ttcggcaagg gcaacacgac
     1441 gtcgaggagc gcctcgctcg cggtgttcac gttcaccgag gtgcgctcgg gaagggcggt
     1501 gacgaacggg cgcagcttcg ccacggtcgc ggcgtcgaac ccccgc
//
LOCUS       DASVII010000449         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_560331,
            whole genome shotgun sequence.
ACCESSION   DASVII010000449 DASVII010000000
VERSION     DASVII010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560331"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VF309_05865"
     CDS             complement(<1..949)
                     /locus_tag="VF309_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX7466147.1"
                     /translation="MQRVKRERLTAWAVASLEKVGVPPPEAKLVGESLVQTSVWGIDS
                     HGVLRLTHYLNRMTIGSISPFAAPTVMRSGPVTAQVHGQDGLGIVHAALAMEVAIEMA
                     KESGVGIVGVGHSSHCGAVGLYTRMAAREHLVGIAMTHSSSVVVPHGGRDKYFGTNPI
                     SIAFPRAGGEPVCLDMATSQVAWNKVLNARIENETLDEGIAVDAEGKPTTDPQRARAG
                     IPLGGPVYGYKGYGLAFMIDLLCGALNGMTFGRHINSMYEELDKPRKIGHLLIAIDPM
                     RFAGGDRLEETLDALIDDLRTQGEILFPGEPELIEQEKRR"
     gene            1016..1636
                     /locus_tag="VF309_05870"
     CDS             1016..1636
                     /locus_tag="VF309_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEX7466148.1"
                     /translation="MNRTLAAWLAALVLAALAASFIAPYDRATWAMEVAPIFIVFPLL
                     AATQRRFPLSDLLYVLIAVHALVLVLGGAYTYARVPLGFWLQDWFHLARNPYDRVGHF
                     LQGLVPALAAREILLRGGYVAGRRMAGFLSLCVAMTVSACYELIEWGAAVALGQGADE
                     FLGTQGDPWDTQWDMFMALIGALCAITLVARLHDARMKRLQGYLPE"
     gene            complement(1621..2475)
                     /locus_tag="VF309_05875"
     CDS             complement(1621..2475)
                     /locus_tag="VF309_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7466149.1"
                     /translation="MRLLRYGAPGREKPGLADKDGNIRDLSKVITQIDDAMLAPRNLA
                     RLTRVKPETLPVVRGNPRMGVPYAGISKFVAIGLNFRDHAQESNLPIRSEPIVFMKAT
                     TCLCGPNDDTIVPKSSQKLDWEVELGIVIGTKAQHVPESKALDYVAGYTVVNDVSERF
                     FQMGPGGQWDRGKGCDTFGPVGPWLVTTDEITDPQSLDMWLDVNGEKRQRGNTRTMIF
                     GCATLVSYVSQFMTLLPGDIITTGTPPGVGMGMKPTPAFLKVGDVVTLGIQGLGEQRQ
                     KIVAYSGK"
     gene            2552..>2652
                     /locus_tag="VF309_05880"
     CDS             2552..>2652
                     /locus_tag="VF309_05880"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX7466150.1"
                     /translation="MILAIDAGNTRIKWGLRDGQAWKAAGAIATRDSA"
BASE COUNT          387 a          898 c          874 g          493 t
ORIGIN      
        1 cacgccgctt ctcctgctcg atcagctcgg gctcgccggg gaagaggatc tcgccttgcg
       61 ttcgcaggtc gtcgatcagc gcgtcgagcg tttcctcgag gcgatccccg cccgcgaacc
      121 tcatgggatc gatcgcgatc agcaggtggc cgatcttgcg cggcttgtcg agctcctcgt
      181 acatggagtt gatgtggcgc ccgaaagtca tgccgttcag cgcgccgcac agcaggtcga
      241 tcatgaacgc gaggccgtag cccttgtagc cgtagaccgg tccgccgagg ggaatgccgg
      301 cgcgcgcccg ctggggatcc gtggtcggct tgccctcggc gtcgacggcg atgccttcat
      361 cgagggtctc gttctcgatg cgcgcgttga gcaccttgtt ccacgcgacc tgcgaagtgg
      421 ccatgtcgag acagacgggc tcgcctccgg cacgcgggaa cgcgatcgag atcgggttgg
      481 tgccgaaata cttgtcgcgc ccgccgtgcg ggacgacgac cgagctcgaa tgcgtcatgg
      541 cgatgccgac gagatgctcg cgcgcggcca tgcgcgtgta gagcccgacc gcgccgcaat
      601 gcgacgagtg cccgacgccg acgatgccca cgcccgattc cttcgccatc tcgatcgcga
      661 cctccatcgc gagcgccgcg tgcacgatgc ccaggccgtc ctgtccgtgc acctgcgccg
      721 tgaccggccc ggagcgcatc accgtcggcg cggcgaaggg cgagatcgac ccgatcgtca
      781 tgcggttgag atagtgcgtg aggcgcagca ccccgtggga gtcgatgccc cacacgctcg
      841 tctgcaccag cgattcgccg acgagcttcg cctccggggg cggcacgccg accttctcca
      901 gcgaagccac cgcccacgcc gtgaggcgct cccgcttcac acgctgcatt ttcttccgag
      961 tgcgcgatct ggtttttcga ccggagccat tctacagtcc gcctacacta tcgcaatgaa
     1021 ccggaccctc gccgcctggc ttgccgcgct ggtgctcgcc gcgctcgcag cctccttcat
     1081 cgcgccttac gaccgtgcca cgtgggcgat ggaggtcgcg ccgatcttca tcgtgttccc
     1141 gctgctggcc gcgacgcagc gccgcttccc gctgagcgat ctcctgtatg tgctgatcgc
     1201 cgtccacgcg ctggtgctgg tcctcggcgg cgcgtacacc tatgcgcgcg ttccgctcgg
     1261 cttctggctg caggactggt tccatctcgc gcgcaatccc tacgatcgcg tcgggcattt
     1321 cctgcagggc ctggtgccgg cgctggcggc gcgcgagatc ctgctgcgtg gcggatacgt
     1381 cgccgggcgg cgcatggccg gatttctctc gctctgcgtc gcgatgacgg tgagcgcctg
     1441 ttacgagctg atcgagtggg gcgcggcggt agcactcggg cagggcgccg acgagttcct
     1501 cggcacccag ggcgatccgt gggacacgca atgggacatg ttcatggcgc tcatcggcgc
     1561 gctttgcgcg atcacgctcg tcgcgcggct gcatgatgcg cgaatgaagc ggcttcaggg
     1621 ctacttgccg gagtaggcga cgatcttctg gcgctgctcg cccaggccct ggatgccgag
     1681 cgtcacgacg tcgcccacct tgaggaacgc gggcgtgggc ttcatcccca tgccgacccc
     1741 gggcggtgtc ccggtggtga tgatgtcccc gggcagcagc gtcatgaact ggctcacgta
     1801 ggagaccagc gtcgcgcagc cgaagatcat ggtgcgcgtg ttgccgcgct ggcgcttctc
     1861 gccgttcacg tcgagccaca tgtcgagcga ctgcgggtcg gtgatctcgt cggtggtgac
     1921 gagccacggg cccaccgggc cgaaggtgtc gcagcccttg ccgcggtccc actggccgcc
     1981 gggccccatc tggaagaagc gctcggagac gtcgttcacc accgtgtagc cggcgacgta
     2041 gtcgagcgcc ttcgattcgg ggacgtgctg cgccttggtg ccgatcacga tgccgagctc
     2101 cacctcccag tcgagcttct gcgagctctt cggcacgatc gtgtcgtcgt tcggcccgca
     2161 caggcacgtg gtcgccttca tgaagacgat cggctcgctc cggatcggca ggttcgactc
     2221 ctgcgcgtgg tcgcggaagt tcaggccgat ggccacgaac ttgctgatcc ccgcataggg
     2281 aactcccatg cgcggattgc cgcgcacgac cggcagcgtc tccggcttca cgcgcgtcag
     2341 ccgcgcgagg ttgcgcggcg cgagcatcgc gtcgtcgatc tgggtgatca ccttcgagag
     2401 gtcgcggatg ttgccgtcct tgtccgcgag gccgggcttt tcacggccgg gggcgccata
     2461 acgcaagagt ctcatttttg ttctcccgtt ataaaaagtg gtgtcaggta caacttaaaa
     2521 agtcgtacct gacaccactt tttggaacca catgattctg gccatcgatg ccggcaacac
     2581 ccgcatcaag tggggcctgc gcgatggcca ggcctggaag gcggccggcg cgatcgccac
     2641 gcgcgactca gc
//
LOCUS       DASVII010000450         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_56902, whole
            genome shotgun sequence.
ACCESSION   DASVII010000450 DASVII010000000
VERSION     DASVII010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56902"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VF309_05885"
     CDS             <1..1308
                     /locus_tag="VF309_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX7466151.1"
                     /translation="RLAPRASCLFSLFMTAAVAQTLPYETKAINLGVVNCASSLCHGS
                     ASSWKDSNILQNEYITWSRVDKHATRAYQVLFNERSKRIAQNLGLKEPAHQAKLCLDC
                     HAYNPPPAQRGERFKVADGVSCEACHGPAEPWIRSHVAPDATHADNLAHGLYPTSEPV
                     AQARLCLSCHFGNQDRFVTHRMMGAGHPRISFELDTFSQTQPAHFIADADWQKRKGAW
                     DPARIWAVGQALAAAELLDVMLDPKRSRDGLFPELVVFDCHACHHSMTDLRWSPRSGT
                     SPGRIRLNDSNLLMLRQIVRRALPGDEANDFAQRVNDLHKAVAGDGGDPLETARSLRA
                     SLDGVVRQLARRSFNGEDLRAMLAGLVGDGLNGRYRDYSGAEQATMAIGSLMGYLSRR
                     GELKNARAAAEALDRLYATVKDDDKFRPDRFEAALDALGKTIP"
     gene            1309..>2750
                     /locus_tag="VF309_05890"
     CDS             1309..>2750
                     /locus_tag="VF309_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7466152.1"
                     /translation="MTSFDQRTEFRWYDIGIVGSGPAGLSAAAHAAELGVAHVLIEAE
                     PHLSNTIHKYQKGKHVMAEPQVLPLRSAITFGAGKREAVLGAWTSEVAQKGVNVRHGC
                     DVTAIRGGGEGFEVQTRGGETFLCRKVILAIGVQGNLRKLGVAGEDLEGVQYQLDDPG
                     EYEDETIVVVGAGDAAIENALALADRNRVILINRNEEFARCKEGNLSLVLKAIKEGRI
                     DCRYGTSATKVERGSGDPPLAFGVKTPDGPDLIECNRIIARLGATPPRKLVESFGVQF
                     PSKDPNSVPALSDTYESNVKGLYIIGALGGYPLIKQAMNQGYEAVEFALGRTVAPADE
                     PLLAKKFANFSRRKTVSQVLDVIQANVPLLEGINRLQLREFMLESELATPAEGDVVFL
                     RNDYTNSFFMVVSGEVKVEVLREGEPSSWFPLGPGEFFGELGLISGRRRSSTVKAGPD
                     CVLIEAPRRAMLKLIASVESIRKKVDEKFL"
BASE COUNT          480 a          939 c          928 g          403 t
ORIGIN      
        1 cgtctcgcgc ctcgtgcctc gtgcctgttc tcgctgttca tgaccgcggc cgtcgcccag
       61 acccttccct acgaaaccaa ggccatcaac ctgggggtgg tcaactgcgc gagcagcctc
      121 tgccacgggt cggcgtcgtc gtggaaggac tcgaacatcc tgcagaacga gtacatcacg
      181 tggtcgcgcg tcgacaagca cgcgacccgg gcgtaccagg tcctcttcaa cgagcgctcg
      241 aagcgcatcg cgcagaacct gggattgaag gagccggcgc accaggcgaa gctctgcctc
      301 gactgccacg cgtacaaccc gccgccggcg cagcgcggcg aacgcttcaa ggtcgccgac
      361 ggcgtgagct gcgaggcgtg ccacggtccg gcggagccgt ggatccgctc ccacgtggcc
      421 cccgacgcga cccatgccga caacctggcc cacggcctct atcccaccag cgagccggtg
      481 gcccaggcga ggctgtgcct gtcctgccac ttcggcaacc aggaccggtt cgtgacgcac
      541 cgcatgatgg gcgcggggca cccgcgcatc agcttcgagc tcgacacctt ctcgcagacg
      601 cagccggcgc atttcatcgc cgatgccgac tggcagaagc gcaagggcgc ctgggacccg
      661 gcgcgcatct gggccgtcgg ccaggcgctc gccgccgccg agctcctcga cgtgatgctc
      721 gatccgaagc gcagccgcga cggcctcttc cccgagctcg tcgtgttcga ctgccacgca
      781 tgccaccatt cgatgacgga cctgcgctgg tcgccgcgct cgggcacgag cccgggacgc
      841 atccggctca acgattcgaa cctcctcatg ctgcgccaga tcgtgcgccg cgcgctgccc
      901 ggcgacgagg cgaacgactt cgcgcagcgc gtgaacgacc ttcacaaggc ggtggcgggg
      961 gacggcggcg atccgctgga aaccgcgcgc tcattgcgcg catccctcga cggcgtcgtg
     1021 cgccagctgg cgcgccggag cttcaacggc gaagaccttc gcgcaatgct cgccgggctc
     1081 gtcggcgacg ggctgaacgg gcgataccgc gactattcgg gcgcggagca ggcgacaatg
     1141 gcgatcggga gcctcatggg ctacctctcg aggcggggcg agctgaagaa cgcccgcgcc
     1201 gccgccgagg cgctcgaccg gctctacgcg acggtgaagg acgacgacaa gttccgcccc
     1261 gatcgtttcg aggcggccct ggacgccctg ggaaagacga ttccatagat gacgagcttc
     1321 gatcaacgca ccgaattccg ctggtacgac atcggcatcg tgggctccgg gcccgcgggc
     1381 ctttccgcgg ccgcccatgc cgccgagctc ggcgtcgcgc acgtgctgat cgaggccgag
     1441 ccgcaccttt cgaacacgat ccacaagtac cagaagggca agcacgtgat ggcggagccg
     1501 caggtcctgc cgttgcgcag cgcaatcacg ttcggcgcgg gcaagcgcga ggcggtgctc
     1561 ggcgcctgga cgagcgaggt cgcacagaag ggcgtgaacg tccgccatgg ttgcgacgtg
     1621 acggcgattc gcggcggagg cgagggcttc gaagtgcaga cgcgcggcgg agagacgttt
     1681 ctctgccgca aggtgatcct cgcgatcggc gtgcagggaa acctgaggaa gctcggcgtt
     1741 gccggcgagg acctcgaagg cgtgcagtac cagctcgacg atcccggcga atacgaggac
     1801 gagacgatcg tcgtggtcgg cgcgggcgat gccgcgatcg agaacgcgct cgcgctggcc
     1861 gacaggaacc gcgtgatcct catcaaccgc aacgaggaat tcgcccgctg caaggaaggc
     1921 aacctctcgc tcgtgctgaa ggcgatcaag gagggccgca tcgattgccg ctacggcacc
     1981 tcggcgacga aggtcgagcg cgggagcggc gatccgccgc tcgccttcgg ggtgaagacg
     2041 cccgacggcc ccgacctcat cgaatgcaac cgcatcatcg cgcgcctggg cgcgacgccg
     2101 ccgaggaagc tcgtggaaag cttcggcgtg cagttcccga gcaaggatcc gaactcggtg
     2161 ccggcgcttt ccgataccta cgagtcgaac gtgaaggggc tctacatcat cggagcgctg
     2221 ggcggctatc ccctcatcaa gcaggcgatg aaccagggct acgaggccgt ggagttcgcg
     2281 ctcggcagga ccgtggcccc ggccgacgag cccctcctcg cgaagaagtt cgcgaacttc
     2341 tcgcgccgca agacggtctc gcaggtgctc gacgtgatcc aggccaacgt gccgctcctc
     2401 gaggggatca accgcctgca gctgcgcgag ttcatgctcg agagcgagct cgccactccc
     2461 gcggaaggcg acgtcgtctt cctgcgcaac gactacacca actcgttctt catggtcgtt
     2521 tccggggaag tgaaggtgga agtcctgcgc gagggcgagc cgtcgagctg gttcccgctg
     2581 gggcccggtg aattcttcgg cgagctcggc ctcatctcgg ggcgacggcg ctcgagcacc
     2641 gtgaaagcgg gacccgactg cgtcctcatc gaggcgccgc ggcgcgcgat gctgaagctc
     2701 atcgcgtcgg tcgagtcgat ccgcaagaag gtcgacgaga agttcctcaa
//
LOCUS       DASVII010000451         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_416457,
            whole genome shotgun sequence.
ACCESSION   DASVII010000451 DASVII010000000
VERSION     DASVII010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416457"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF309_05895"
     CDS             <1..663
                     /locus_tag="VF309_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7466153.1"
                     /translation="LPLWLQQYMGYTATWAGLVTAPVGVLAIILSPWVGKNVTRIDPR
                     KLATVSFIVFALVLWMRSHFNTQADVMTILIPTVIQGAAMAFFFIPLQAIVFSGLTPD
                     RMPSAAGLSNFVRITAGAIGTSLFTTFWENRAVLHHAQLAESINRGNNAAIQSLDQLA
                     ANGFSYDQSLASINRLIDQQAYTMAVNDMFYLSSMLFLLMIGVIWLSKPKQGAAAGGG
                     AH"
     gene            715..1209
                     /locus_tag="VF309_05900"
     CDS             715..1209
                     /locus_tag="VF309_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466154.1"
                     /translation="MSIAIAAVMLCGRAAAQDPPAQDPPGMKNWFGDPFFQLTREIDS
                     CPRPLGPYVTEEEALRYSHHRAERGTRCYQEKRCRLPSSYDYDREIAARIQGDVAEKR
                     LPLPRSTLWVLVQGRRVWIQGCVAPGYRRGALSAKLKRVPDVELAMEEVRIGAGGAIP
                     YRTR"
     gene            complement(1222..>1518)
                     /locus_tag="VF309_05905"
     CDS             complement(1222..>1518)
                     /locus_tag="VF309_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466155.1"
                     /translation="DWLPRGPIERFFRRQQKDDFLASDFAGKEENLYFLFGMLTSAAR
                     ARLDAQLRKLREEFAGLHRESLAAPFEQRRGACLLVAQREWEPCSFEALRRAPV"
BASE COUNT          242 a          518 c          492 g          266 t
ORIGIN      
        1 ctgccgctct ggctgcagca gtacatgggc tacaccgcga cgtgggcggg gctcgtgacg
       61 gcgcccgtgg gcgttctcgc gatcatcctc tcgccatggg tcggcaagaa cgtgacccgc
      121 atcgatccgc gcaagctcgc cacggtgtcc ttcatcgtct tcgccctcgt cctgtggatg
      181 cgctcgcact tcaacacgca ggccgacgtc atgacgatcc tcattcccac cgtgatccag
      241 ggcgcggcca tggccttctt cttcatcccg ctgcaggcga tcgtgttctc ggggctcacg
      301 ccggatcgca tgccgtcggc ggcgggcctg tcgaacttcg tgcgcatcac ggccggagcc
      361 atcgggacgt ctctcttcac cacgttctgg gagaaccgcg cggtcctgca tcacgcccag
      421 ctggccgagt cgatcaaccg cggcaacaac gcggcgatcc agagcctgga ccagctcgcg
      481 gcgaacgggt tcagctacga ccagtccctg gcaagcatca accgcctgat cgaccagcag
      541 gcgtacacga tggccgtgaa cgacatgttc tacctctcgt cgatgctgtt cctgctgatg
      601 atcggcgtga tctggctgtc caagcccaag cagggagcgg ccgccggcgg cggggcccac
      661 taggtcccga gggctcccgc ggtgctccga tccgcaatga cgagccgcct gcgagtgtcc
      721 atcgcgatcg ccgcagtcat gctttgcggc cgcgcggccg cgcaggatcc tcccgcgcag
      781 gatccgccag ggatgaagaa ctggttcggc gatccgttct tccagctcac ccgcgagatc
      841 gattcgtgcc cgcggccgtt gggtccatac gtgaccgagg aggaagcgct tcgctattcg
      901 catcatcgcg cggagcgcgg aacgcgctgc taccaggaga agcgctgccg cctgccgagc
      961 tcctacgact acgaccgcga gatcgccgcg cgcatacaag gcgatgtcgc ggagaagcgc
     1021 ctgccgttgc cgcgctccac gctctgggtg ctggtgcagg gacgccgcgt gtggatccag
     1081 gggtgcgtcg cgccgggcta ccgtcgcggc gctttgagcg cgaagctcaa gcgcgttccc
     1141 gacgtcgagc tcgcgatgga agaagtgcgg atcggcgcgg gcggcgcgat tccctatcgg
     1201 acgcgctgag cgggattcga gctagaccgg cgctctgcgc agtgcctcga agctgcaagg
     1261 ctcccattcg cgttgcgcca cgagcaggca ggcgccgcgg cgttgctcga aaggtgcggc
     1321 gaggctttcg cgatggagcc ccgcgaattc ctcgcgaagc ttcctcagct gcgcatcgag
     1381 cctcgccctc gcggccgatg tgagcatgcc gaagagaaaa tagaggttct cctccttgcc
     1441 ggcgaaatcg ctcgccagga agtcgtcctt ctgttgcctt cgaaagaatc gctcgatcgg
     1501 cccgcggggc agccagtc
//
LOCUS       DASVII010000452         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_416592,
            whole genome shotgun sequence.
ACCESSION   DASVII010000452 DASVII010000000
VERSION     DASVII010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416592"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1229)
                     /locus_tag="VF309_05910"
     CDS             complement(21..1229)
                     /locus_tag="VF309_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7466156.1"
                     /translation="MTIARSRRVAGRAVFILAAALAGPLHAAKSQPPSEEDLRELRGR
                     IEKLQGDLTAAEESRGEAADQLKASGKAVSEAQRALFELAQRRRTLEGELAEIVRRDR
                     ETRANVTEQEALAAKLLRLQYQQGAPDRLRLALEGRDAATVARHIAYYGYIQRARAGI
                     IGELKRKEETLAALGKDAMARRDELKQNERQQADETRKLERERAARAAVLARLAGEIQ
                     RSRREIGKLRRDEARLSRLVEEIARALAAKPAEGTRKEGRAVDRIADSSSSLKPFTSL
                     KGRLRLPVRGELMNRYGGAREETGATWKGLFIRCVSGETVHAVADGRVVYADWLRGFG
                     NLLILDHGTGYMSLYANNDGLLRQVGESVHGGDPIAHVGASGDNVESGLYFELRRDGK
                     PFDPLRWVAP"
     gene            complement(1235..>1630)
                     /locus_tag="VF309_05915"
     CDS             complement(1235..>1630)
                     /locus_tag="VF309_05915"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX7466157.1"
                     /translation="DVAPPALDPSDERNAARDPRYRSLRPGQVPLSECLKDTVARVVP
                     YWKDRIAPRVAAGERVLVAAHGNSLRALIKYFDALSDEAIVGVNVPTGIPLVYELDDS
                     LHALRNYYLGDAQAVAAAQHSVAAQGKAK"
BASE COUNT          220 a          597 c          541 g          272 t
ORIGIN      
        1 aggcgtcgcg ctcaccctgg ctagggtgct acccacctca acggatcaaa cggctttccg
       61 tcgcgacgca gctcaaagta tagacccgat tcgacgttat cacccgatgc tcccacatgc
      121 gcaatcgggt ctccgccatg cacgctttcg ccaacctgtc gaagcaatcc gtcgttgttg
      181 gcgtagaggc tcatgtaccc cgttccgtgg tcaagaatca agaggttacc gaagccgcgc
      241 agccagtcgg catagaccac gcggccgtcg gccacggcgt ggaccgtttc acctgaaaca
      301 cagcggatga agaggccctt ccaggtggca cccgtttcct cgcgggcccc gccgtagcgg
      361 ttcatgagct cgccccgcac cggaaggcgc aggcgcccct tgaggctcgt gaaaggcttc
      421 agggacgagg acgaatcggc gatgcgatcg acggcgcggc cctctttgcg cgtaccttcg
      481 gcgggcttcg ccgcgagcgc gcgcgcgatc tcctcgacca gcctcgacag gcgcgcctcg
      541 tctcgcctga gcttgccgat ctcgcggcgg cttcgctgga tctcgcccgc gaggcgcgcg
      601 agcaccgcgg cgcgcgccgc gcgctcgcgc tcgagcttgc gcgtctcgtc cgcctgctgc
      661 ctctcgttct gcttcagctc gtcgcgccgc gccatcgcgt ccttgccgag cgcggcgagc
      721 gtctcctcct tgcgcttcag ctcgccaatg attcccgcgc gcgccctctg gatgtacccg
      781 tagtaggcga tgtgtcgcgc caccgtcgcc gcgtcgcgcc cctcgagcgc gagcctcagc
      841 cggtcgggcg cgccctgctg gtactgcagg cgcagcagct tggcggcaag cgcttcctgc
      901 tccgtgacgt tcgcgcgggt ctcgcgatcg cggcggacga tctccgcgag ctcgccctcg
      961 agcgtgcggc ggcgctgcgc gagctcgaag agcgcgcgct gggcttcgct caccgccttg
     1021 ccgctcgcct tcagctggtc cgccgcctcg ccgcgcgatt cctccgccgc cgtgaggtcg
     1081 ccctgcagct tctcgatgcg cccgcgcagc tcgcgaagat cctcctcgct ggggggctgc
     1141 gatttcgccg cgtggagcgg gcccgcgagg gccgccgcga ggatgaacac cgcccgcccc
     1201 gccacgcgcc gcgagcgcgc gatcgtcatc gcggctattt cgccttcccc tgggccgcga
     1261 ccgagtgctg cgcggcggcg acggcctgcg cgtcgccgag atagtagttg cgaagcgcgt
     1321 gcagcgaatc gtccaattcg taaacgagcg ggatgccggt gggaacgttc actccgacga
     1381 tcgcctcgtc gctcagcgcg tcgaagtact tgatgagcgc gcgcaggctg ttgccgtggg
     1441 cggcgacgag aacgcgctcg ccggcggcga cgcgcggcgc gatgcggtcc ttccagtaag
     1501 gaaccacgcg cgccaccgtg tccttcaggc attcactgag cggcacctgg ccgggccgca
     1561 gcgaccggta gcgcggatcg cgcgcggcgt tgcgctcatc ggaaggatcg agcgcgggcg
     1621 gcgcaacgtc
//
LOCUS       DASVII010000453         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_596285,
            whole genome shotgun sequence.
ACCESSION   DASVII010000453 DASVII010000000
VERSION     DASVII010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596285"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VF309_05920"
     CDS             complement(<1..602)
                     /locus_tag="VF309_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7466158.1"
                     /translation="MTVTLKEIEAAARVIEGRVVRTPCLHSRTLSDITGAEVYLKFEN
                     HQFTASFKERGALNKLASLDAAQRAKGVIACSAGNHAQGVAYHAHRLGIPAAIVMPRY
                     TPWVKVEQTQRHGAEVILEGDNFDGAKDFAMAAAVERGLTLVHPYNDEKVIAGQGTAG
                     LEILQEHPELDALIVAIGGGGLISGISIAAKSMKPAIEVFG"
     gene            complement(599..>1849)
                     /locus_tag="VF309_05925"
     CDS             complement(599..>1849)
                     /locus_tag="VF309_05925"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF001933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX7466159.1"
                     /translation="VLVLGAGVIGVSTAWYLTQHGHEVTVIDRREGAGLETSFANGGQ
                     VSVCHAEPWANPGAPAKILQWIWRDDAPLLFRWRMDPHQWSWGIRFLAECLPARTRAN
                     IGEIVRLSLYSRASLQALRAETRLEYDQLELGILHYYTERKEFDRAVAAASIMKEYGL
                     DREVKSVDEAMKIEPALASARDRIVGATYTSTDESGDAHLFTSRLAKMAAEKGARFLY
                     GRTIRRILAGGGRVGGVAVTHGEGIDEVMTSDAYVVALGSYSPLLTRPIGLDLPVYPA
                     KGYSASLPILDASKAPRVSLTDDEAKIVVTRLGDRMRIAGTAELSGYSTELNTVRCEA
                     LTRRVGEMFPGAGDYGKAEFWTGLRPSTPSNVPLIGATRYRNLYLNTGHGTLGWTMAA
                     GSGRALADIISGRKPEVDFRFTSP"
BASE COUNT          280 a          617 c          656 g          296 t
ORIGIN      
        1 ccgaacacct cgatcgcggg cttcatcgat ttcgcggcga tggagattcc cgagatgagg
       61 ccgccgccgc cgatcgccac gatgagcgca tcgagctcgg gatgctcctg caggatctcg
      121 agccccgccg tgccctgccc ggcgatcact ttctcgtcgt tgtacggatg cacgagcgtg
      181 aggccgcgct cgaccgcggc ggccatcgcg aaatccttgg cgccgtcgaa gttgtcgccc
      241 tcgaggatca cctccgcgcc gtggcgctgc gtctgctcga ccttaaccca cggcgtgtag
      301 cgcggcatca cgatcgccgc gggaatgccg aggcgatgcg cgtgataggc gacgccctgc
      361 gcatggttgc ccgcggagca ggcgatcacg cccttcgcgc gctgcgccgc atcgagcgac
      421 gcgagcttgt tcagcgcgcc gcgctccttg aacgacgccg tgaactggtg gttctcgaac
      481 ttgaggtaga cctcggcgcc ggtgatgtcg gagagcgtgc gcgagtgcag gcacggcgtg
      541 cgcacgaccc ggccctcgat cacccgggcg gcggcctcga tttccttgag cgtgacggtc
      601 atggggaggt gaagcggaag tcgacttcgg gcttgcgacc gctgatgata tccgcaagcg
      661 cgcgccccga cccggcggcc atggtccacc cgagcgtgcc gtggcccgtg ttgaggtaga
      721 ggttccggta gcgcgtcgcg ccgatgagag gcacgttcga gggcgtggag gggcgaaggc
      781 cggtccagaa ctccgccttg ccatagtcgc ccgcgccggg aaacatctcg ccgacccggc
      841 gggtgagcgc ctcgcagcgc accgtgttga gctcggtcga gtagcccgac agctccgccg
      901 ttcccgcgat gcgcatccgg tctcccaggc gggtgacgac gatcttcgcc tcgtcgtcgg
      961 tgaggctcac gcgcggggcc ttcgacgcgt cgaggatcgg gagcgaagcc gagtagccct
     1021 tcgcgggata caccggcagg tcgagcccga tggggcgcgt gaggagcggc gaatagctgc
     1081 cgagcgcgac gacgtaggcg tcggatgtca tcacctcgtc gatgccttcg ccatgggtca
     1141 ccgcaacgcc gcccacccgg ccgccgcccg cgagaatgcg ccggatagtc cgcccgtaga
     1201 ggaaacgcgc gcccttctct gccgccatct tcgcgagcct cgaggtgaag aggtgcgcgt
     1261 ctcccgattc gtcggtggac gtataggtcg cgccgacgat gcgatcgcgg gccgaggcga
     1321 gcgcgggctc gatcttcatg gcctcgtcga ccgatttcac ctcgcggtcg aggccgtact
     1381 ccttcatgat cgaggccgcg gccacggcgc gatcgaactc cttgcgctcg gtgtagtagt
     1441 gcaggatgcc gagctcgagc tggtcgtact cgaggcgggt ctccgccctc agggcctgca
     1501 gggaagccct gctgtagagc gacagccgca cgatctcgcc gatgttggcg cgcgtcctcg
     1561 cgggaaggca ttcggcgagg aagcggatcc cccaggacca ttggtgcgga tccatccgcc
     1621 agcggaagag aagcggcgcg tcgtcgcgcc agatccactg caggatcttg gcgggcgcgc
     1681 cgggattggc ccagggctcg gcgtggcaca cggagacctg gcccccgttc gcgaacgagg
     1741 tctcgaggcc ggcgccctca cggcggtcga tcaccgtgac ttcgtggccg tgctgggtga
     1801 ggtaccaggc ggtggagacg ccgatcacgc cggcgccgag gacgaggac
//
LOCUS       DASVII010000454         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_488381,
            whole genome shotgun sequence.
ACCESSION   DASVII010000454 DASVII010000000
VERSION     DASVII010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488381"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VF309_05930"
     CDS             complement(<1..942)
                     /locus_tag="VF309_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX7466160.1"
                     /translation="MRRWLLPEHIVDVLPAEARAIERLRRGILDLFESHGYELVAPPL
                     LEYVESLLSGTGRDLDLVTFKLVDQLSGRMMGVRADHTPQVARIDSHLLNRQGVARLC
                     YCGSVLHTVPAGMTKMREPIQIGAELYGHSGLEADLEIVQLLIAALKKAGIARIHLDL
                     GHPSIYRALTEKSGLNAEDSEALFHAVQQKDAPAARELGGEAVAALTALNGGRDVIAR
                     ARKSLPSLPAVGVALNALERLAAQCAAPGVEISVDLAELGGFNYESGLVFAAFTPGSP
                     DAIARGGRYDEVGASFGRSRPATGFTMDLRQLAALAPK"
     gene            complement(946..1131)
                     /locus_tag="VF309_05935"
     CDS             complement(946..1131)
                     /locus_tag="VF309_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX7466161.1"
                     /translation="MSDIVLPALALMLVIEGILPFTSPRLWRETFKRLIEMTDGQIRF
                     AGLMSMLLGLVVLFLAT"
     gene            complement(1128..>1897)
                     /gene="hflC"
                     /locus_tag="VF309_05940"
     CDS             complement(1128..>1897)
                     /gene="hflC"
                     /locus_tag="VF309_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX7466162.1"
                     /translation="IDVQTEPGLYFLVPLMQNVKLFDTRIQTLEAREPERFITSENRN
                     VLVDSFVKWRVVDVRQYYVSVRGDSLQAESRISQTVNDALRAEFAKRTVHDVVSGERD
                     QIMRTVAERVDADLRRIGVAVIDVRLKRVDFVPEISSNVYDRMNSERKRVANELRATG
                     QAEGEKIKADADRQRQVIVAEAYRDAQKTKGEGDAIASRTYAEAFGRNPEFFSFYRSM
                     EAYRSSLRSKGDVMVLDPSSDFFKYLKNPGRGKTSAP"
BASE COUNT          289 a          647 c          656 g          305 t
ORIGIN      
        1 cttcggcgcc aatgccgcga gctggcgcag gtccatcgtg aatcccgtcg cgggccgcga
       61 gcgcccgaag ctcgcgccca cctcgtcgta gcgcccgccc cgcgcgatcg catcggggct
      121 gcccggcgtg aacgcggcga acacgagccc gctctcgtaa ttgaagccgc ccagctccgc
      181 caggtccacc gagatctcga cgccgggcgc cgcgcactgc gcggcgaggc gttcgagcgc
      241 gttcaaggcc acgcccaccg cgggcaggct cggcaggctc ttgcgcgccc tggcgatcac
      301 gtcgcgcccg ccgttcaagg ccgtgagcgc cgcgaccgcc tcgccgccca gctcgcgggc
      361 cgcgggagcg tccttctgct gcaccgcgtg gaagagcgcc tccgaatcct cggcgttgag
      421 ccccgacttc tcggtgagcg cgcgatagat gctcgggtgc ccgagatcga gatggatgcg
      481 cgcgatcccg gccttcttca gcgccgcgat gagcagctgc acgatttcca ggtccgcctc
      541 caggcccgaa tggccgtaga gctcggcgcc gatctggatc ggctcgcgca tcttggtcat
      601 tcccgcgggc acggtgtgca ggacgcttcc gcaatagcac aggcgcgcca cgccctggcg
      661 gttgaggagg tgcgagtcga tgcgcgcgac ctgcggcgtg tggtcggcgc gcactcccat
      721 catgcggccc gagagctggt ccaccagctt gaaggtcacg agatcgaggt cgcgcccggt
      781 ccccgagagg agcgactcga cgtactcgag cagcggcggc gcgacgagct cgtagccgtg
      841 ggattcgaac aggtcgagga tcccgcggcg caggcgctcg atggcgcgcg cttcggcggg
      901 gagcacgtcg acgatgtgct cgggaaggag ccagcggcgc atgggtcagg tcgcgaggaa
      961 gaggaccact aggcccagga gcatcgacat gaggcccgcg aagcggatct gcccgtcggt
     1021 catctcgatg aggcgcttga aggtctcgcg ccacaggcgc ggcgacgtga acgggaggat
     1081 tccttcgatc accagcatga gcgccagggc gggcaggact atgtcgctca cggcgcggag
     1141 gtcttgcccc gccccggatt cttgaggtac ttgaagaagt ccgacgacgg gtcgagcacc
     1201 atcacgtcgc ccttgctgcg caggctgctg cggtacgcct ccatgctgcg gtagaagctg
     1261 aagaactcgg ggttcctgcc gaacgcctcg gcgtaggtgc gcgaggcgat cgcgtcgccc
     1321 tcgcccttcg tcttctgcgc gtcgcgatac gcctcggcga cgatcacctg gcgctggcgg
     1381 tcggcgtcgg ccttgatctt ctcgccctcg gcctggcccg tggcacgcag ctcgttggcg
     1441 acgcgcttcc tctcggaatt catgcggtca tagacgttcg acgagatctc cgggacgaag
     1501 tcgacgcgct tcaggcgcac gtcgatcacc gcgacgccga tgcggcgcaa gtccgcgtcg
     1561 accctctcgg cgacggtgcg catgatctgg tcgcgctcgc ccgagaccac gtcgtgcacg
     1621 gtgcgcttgg cgaactccgc gcgcaatgcg tcgttcaccg tctgggagat gcgcgattcg
     1681 gcctgcagcg agtcgccgcg caccgagacg tagtactggc gcacgtcgac cacgcgccac
     1741 ttcacgaacg aatccaccag gacgttgcgg ttctcgctgg tgatgaagcg ctcgggttcg
     1801 cgcgcctcga gcgtctggat gcgcgtgtcg aagagcttca cgttctgcat cagcgggacg
     1861 aggaagtaga ggcccggctc ggtctgcacg tcgatga
//
LOCUS       DASVII010000455         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_704301,
            whole genome shotgun sequence.
ACCESSION   DASVII010000455 DASVII010000000
VERSION     DASVII010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704301"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="pyrF"
                     /locus_tag="VF309_05945"
     CDS             complement(<1..654)
                     /gene="pyrF"
                     /locus_tag="VF309_05945"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7466163.1"
                     /translation="MDNKRIIVPLDVPDAEAALALAARLDNKLCRVKVGKELFVAAGP
                     AVVARLQERGFEVFLDLKFHDIPNTVAGACRAAARLGVWMMNVHASGGEAMLRAAREA
                     IETVARPPLLIAVTILTSLADEDLAQVGFAGSLMDNVERLARLARASGMDGVVCSARE
                     APLIRKAAGEDFILVTPGIRLKTDDNDDQARTVTPVEAVRLGAHYLVIGRSITKAADP
                     "
     gene            complement(644..>1559)
                     /locus_tag="VF309_05950"
     CDS             complement(644..>1559)
                     /locus_tag="VF309_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7466164.1"
                     /translation="NREDLLPEQRRDASFELSQDYFKAGLLDHAERVLEKLAQSDPSA
                     EVHRHLLDIYIQEKDWEKAIAAAKQLEVSAKRNYQKEIANYYCELASTAHVHGRPEEA
                     MRHLANALEGNRKCVRANLLRGEWLARAGKHAEAIEAWKSIENQDPAYFGLAAEGMVE
                     SYKALGRLGEGLALLRGLQHRYPGLDLLNIVYQATAEHEGDEAAWRLVRDEVRRNPTL
                     VGLDRMIDAELVRAPPERRQDLQLMKDLVHSHAQALAVYLCANCGFKARQFFWQCPAC
                     GGWETFAPRRTAELESAGRHLARMQIGQ"
BASE COUNT          218 a          527 c          542 g          272 t
ORIGIN      
        1 cggatcggcg gccttggtga tggaacgccc gatcacgaga tagtgggcgc cgagtcgcac
       61 ggcttccacg ggcgtcacgg tgcgtgcctg gtcgtcgttg tcgtcggtct tcaatcgaat
      121 tcccggcgtg acgaggatga agtcttcgcc ggcggccttg cggatgaggg gcgcctcccg
      181 cgcggaacac acgacgccgt ccatgccgct cgcccgcgcg aggcgcgcga ggcgctcgac
      241 gttgtccatc agcgatcccg cgaagccgac ctgcgcgagg tcctcgtcgg cgaggctcgt
      301 gaggatcgtc acggcgatca ggagcggcgg acgcgccacg gtctcgatcg cctcgcgcgc
      361 ggcgcgcagc atcgcttcgc cgccgctcgc gtgcacgttc atcatccaca cgccgaggcg
      421 cgccgcggca cggcacgcgc cggcgacggt gttggggatg tcgtgaaact tcaggtcgag
      481 gaacacctcg aagccgcgct cctggagccg cgccaccacc gcggggccgg cggcgacgaa
      541 gagctccttg ccgaccttca cgcggcagag cttgttgtcc aggcgcgcgg cgagcgcgag
      601 cgcggcctcg gcgtcgggaa catcgagcgg aacgatgatg cgcttattgt ccaatttgca
      661 tccttgcgag atggcgcccg gcgctctcga gctcggccgt gcggcgcggg gcgaacgttt
      721 cccatccccc gcatgcgggg cattgccaga agaactgccg cgccttgaag ccgcagttgg
      781 cgcaaaggta cacggcgagg gcctgcgcgt gggagtgcac gaggtccttc atgagctgca
      841 ggtcctggcg cctctcgggc ggggcgcgca ccagctcggc gtcgatcatg cgatcgagcc
      901 cgacgagcgt cgggttgcgc ctcacctcgt cgcgcaccag cctccacgcg gcttcatcgc
      961 cctcgtgctc ggccgtggcc tggtacacga tattcaggag atcgaggccg gggtagcgat
     1021 gctggaggcc gcgaaggagc gcgaggccct cgccgaggcg cccgagcgcc ttgtagcttt
     1081 ccaccatccc ctcggcggcg aggccgaagt acgcggggtc ctggttctcg atcgatttcc
     1141 aagcctcgat ggcctcggcg tgctttccgg cgcgcgccag ccactcgccg cgaagcaggt
     1201 tcgcgcgcac gcatttgcgg tttccttcca gcgcgttcgc gaggtggcgc atcgcctcct
     1261 cggggcgccc gtggacgtgc gcggtgctcg cgagctcgca gtagtagttc gcgatctcct
     1321 tctggtagtt tcgcttggcg ctcacctcga gttgcttcgc ggccgcgatc gccttctccc
     1381 aatccttctc ctggatgtag atgtcgagga ggtggcgatg cacttccgcc gacggatcgg
     1441 attgcgccag cttctcgagc acgcgctcgg cgtggtcgag caggcctgcc ttgaagtaat
     1501 cctgcgacag ctcgaaggaa gcgtcgcgcc gctgctcggg cagaaggtcc tcgcggttc
//
LOCUS       DASVII010000456         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_416702,
            whole genome shotgun sequence.
ACCESSION   DASVII010000456 DASVII010000000
VERSION     DASVII010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416702"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..>1801)
                     /locus_tag="VF309_05955"
     CDS             complement(84..>1801)
                     /locus_tag="VF309_05955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466165.1"
                     /translation="TFAWVALIVGVPASLLRIAPASHVLSAAAWLAAFTTTAACLARD
                     PTFVARAQRILVAVTLVGAPVGLFAASVASQIPREASLISLVALAAGIAIGLAGGPIA
                     TPLGPEQSRWLDAIRSATHAAPRADPATAMTAILSTLRQALGPASATPELWQLDPPAL
                     MTVDRAGYSRLETGMEVPKLLLELALGEPEQTVRTVVLEALEVRRADVRPALEWLHDR
                     GAMSLTLLTEEEGPTGALVLPSAARTSPLSLEEVRALRALADRMTALLGMSSALARAQ
                     TRQVEAQKTADREEDRALHLEHLLGSGRERHEAFARKLARSALLAPYSPAARLCIQEL
                     QRLGNLGAPVSLLTPPGIDPVPYAAVVHGASARRGGPFVVVDATGSDEQQEEAWRSPA
                     LSPLCLADGGTLVVLSVAALSEGAQTFLAHALSERRSPAGHAAPLDVSLIVSVSTTVD
                     VLVATGRLDPSLADRLGDRSVPLPPLGARGEDLHALMGDRLARLGVRMRGHAMGLDPR
                     ALGRLVEHGWPGNDIELDDVLTRAIVVAEGDVVTIAHLDQIGFVAAPPTARRGSRPPS
                     VHPRV"
BASE COUNT          311 a          579 c          679 g          232 t
ORIGIN      
        1 tcgggcctga cgacgaaggg cgggactctg cgcaggcgcc gaggagcagg gcaacccaca
       61 tgcacgcgca acgcatgagg ggactatacc cgcggatgga cgctcggggg acgggatccg
      121 cgccgagccg tgggcggtgc agcgacgaaa ccgatctgat cgaggtgcgc gatcgtgacc
      181 acgtctccct ccgcgacgac gatggcgcgg gtcagcacgt cgtcgagctc gatgtcgttg
      241 ccgggccatc cgtgctcgac gagccgaccg agcgcgcgag gatcgaggcc catcgcgtgc
      301 ccgcgcatgc gcactccaag cctcgccagg cgatcgccca tgagcgcatg aaggtcttcc
      361 cctcgcgcgc cgaggggagg cagcgggacg gagcgatcgc cgagacgatc ggcaagcgag
      421 ggatcgagac gccccgtggc gaccagaacg tccacggtcg tggagaccga cacgatcaaa
      481 gatacgtcga gaggtgcggc gtggccggca ggcgatcgcc tctcggacag cgcgtgcgcg
      541 aggaacgtct gcgctccttc ggagagtgcc gcaaccgaga gcacgaccag ggttccaccg
      601 tcggcgaggc agagcggcga tagcgcggga cttcgccacg cttcctcttg ttgctcgtcg
      661 gagccagtgg catcgaccac cacgaagggc cccccacgcc gagcgctggc tccgtgaacg
      721 acggccgcat aggggacggg atcgattcct ggcggcgtga gcaggctcac cggtgcgccg
      781 agattgccga gacgttggag ctcctggatg caaagacgcg cggcagggct gtacggcgcg
      841 aggagtgccg accttgcgag cttgcgtgcg aaggcttcgt gccgctcacg ccccgaccct
      901 agaaggtgct cgaggtgcag ggcgcggtcc tcctcgcgat ctgcggtctt ctgcgcctcg
      961 acctggcgag tctgggcgcg ggcgagggcc gaagacatgc ccaagagggc ggtcatgcga
     1021 tcggccaacg cgcgcagcgc tcgaacttct tcgagcgaaa ggggcgaggt gcgcgccgct
     1081 gacggcaaga ccagggcgcc ggtggggcct tcttcctcgg tgaggagcgt caatgacatt
     1141 gccccacggt catgtagcca ttcgagagcc gggcggacgt cggcccggcg gacctcgagc
     1201 gcctcgagca cgacggtgcg cacggtttgc tccggctcgc ccagcgccag ctcgaggagc
     1261 agcttgggca cctccatgcc cgtctcgagc cgcgagtatc cggcgcgatc gacggtcatg
     1321 agggccggcg gatcgagctg ccagagctcg ggcgtcgcgc tggcgggccc tagcgcttga
     1381 cggagcgtcg acaggatggc cgtcatggcc gtggctggat cggcgcgcgg cgctgcgtgg
     1441 gtggcgctcc tgatggcgtc gagccagcgc gactgctcgg gtccgagagg tgtcgcgatg
     1501 gggccgccgg ccaggccgat ggcaatgccg gcggcgaggg caacgagact gatgagcgac
     1561 gcctcgcgag ggatctggga agccaccgag gccgcgaaca gcccgaccgg cgcgccgacg
     1621 agcgtcaccg cgacgagaat gcgctgcgcg cgagcgacga acgtgggatc gcgcgccaga
     1681 caggcggccg tggtcgtgaa ggcagcgagc cacgcagcgg ccgacagaac gtgggaggca
     1741 ggcgcgatgc ggagaagtga cgccggtaca cccacgatga gtgctaccca agcgaacgtc
     1801 a
//
LOCUS       DASVII010000457         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_164695,
            whole genome shotgun sequence.
ACCESSION   DASVII010000457 DASVII010000000
VERSION     DASVII010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164695"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF309_05960"
     CDS             <1..199
                     /locus_tag="VF309_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7466166.1"
                     /translation="VGAALIGWVSGEMIISDPAIKDWIDANMSILHWIAPISGAVLVI
                     GVGKTLEHMASKKESPDIALP"
     gene            216..512
                     /locus_tag="VF309_05965"
     CDS             216..512
                     /locus_tag="VF309_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466167.1"
                     /translation="MSARIQRKKPPGGPREGTPSSIPADYVEPPAANAPIQLCAFCLD
                     HPSGSPGHSGLAQQVHKIPTNHRSYVRLACVFCGAAWVRRRLNARTFEWLRIAD"
     gene            779..1126
                     /locus_tag="VF309_05970"
     CDS             779..1126
                     /locus_tag="VF309_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7466168.1"
                     /translation="MKVPTAGPRGQRYRRSLSEKIAIVRESMLPGMTAAHVAKRHQIA
                     LNVLYYWRKVYRELVDSDLTANAAPAGGESSDVADLQLQVRNLERLLGQRTLEVALLR
                     EKLGLREKEDVDE"
     gene            1119..1445
                     /locus_tag="VF309_05975"
     CDS             1119..1445
                     /locus_tag="VF309_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466169.1"
                     /translation="MNSVRQRRHVHLGERLDAIETIRRGELSLGQAAAQLGVTPAQVL
                     HWMNIHADDRAVRVEDVVVPADVQRLTRRAQRLVALISVADSTIRTLNRQLSDAAPSR
                     RRALAA"
     gene            1601..>2144
                     /locus_tag="VF309_05980"
     CDS             1601..>2144
                     /locus_tag="VF309_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7466170.1"
                     /translation="MKMKVKKLSTAVIHALGAGAMIAAFTPAAVAQQPPPAPKERIEV
                     TGSNIKRVEGESALPVTVITREEIDKSGATNAMELLNLIGSNASAGSVLAASTIGATT
                     FSAQTANLRGLTGGRTLVLINGKRVNGFAGEVNGVQGVNLSVIPFAAIDRVEILKDGA
                     SAVYGSDAIAGVINFIMRSDY"
BASE COUNT          387 a          739 c          688 g          330 t
ORIGIN      
        1 ggtcggcgcg gcgctgatcg gctgggtctc gggcgagatg atcatctccg acccggccat
       61 caaggactgg atcgacgcga acatgtcgat cctgcactgg atcgcgccga tctccggcgc
      121 cgtcctcgtg atcggggtcg gaaagacgct cgagcacatg gcctcgaaga aggaatcccc
      181 ggacatcgcg ttgccctgac accgcatacc cgtcgatgag cgccaggatc cagcgcaaga
      241 agccgcccgg cggcccacgg gagggcacgc cgagctccat cccggccgac tacgtcgagc
      301 cgcccgccgc gaacgccccg atccagctct gcgccttctg cctcgaccat ccttccgggt
      361 cgccgggcca ctcggggctc gcgcagcaag tgcacaagat ccccaccaac catcgctcgt
      421 acgtccgcct cgcgtgcgtg ttctgcggcg cggcctgggt gcggcgccgc ctcaacgccc
      481 gcaccttcga gtggctgcgc atcgccgact gaagcgccgc gcccaggcac acgcggcccg
      541 cgccgaggag cacaccgaca cccgctgacg acctccagcc cgaaacggcc ggcacgcatt
      601 cttgacgaag cgtgaccggc cgtttcacat tgttgcgcgc gcagtgttac caaatctgcc
      661 aattggttgt atgtgcaact acccgatgct agtttcggtg tggtgccgat cacacaacca
      721 cggtgaatgg attgactccc atcgaagcag cgccgccccg cgcgcgggcc cgcgcccggt
      781 gaaggttccc accgcgggcc cgcgcggcca gcgctaccgg cgaagcctct cggagaagat
      841 cgcgatcgtt cgcgaatcga tgctgcccgg gatgacggcg gcccatgtgg cgaaaaggca
      901 tcagatcgcg ctgaacgtcc tttattactg gcgcaaggtc taccgcgagc tcgtcgactc
      961 cgacctcacg gcgaacgccg cgcccgcggg cggcgagagc tccgacgtgg ccgatctgca
     1021 actgcaggtg cgcaacctcg agaggctcct cggccaacgc acgctcgagg tggcgctgct
     1081 gcgcgaaaaa ctcggacttc gtgaaaagga ggatgtcgat gaatagcgtg agacaacggc
     1141 gccacgtgca cctgggcgag cgcctcgatg ccatagagac catccgccgc ggcgagctct
     1201 cgctcggcca ggcggcggcg cagctcggcg tgacccccgc gcaggtgctg cactggatga
     1261 acatccacgc cgacgaccgc gccgtgcgcg tcgaggacgt ggtggtgccg gccgacgtgc
     1321 agcggctcac gcggcgcgcg caacgcctcg tcgcgctcat ttccgtggcc gattcgacca
     1381 ttcgcacgct gaaccggcaa ctctccgacg ccgcgccttc gcgccggcgg gcgctggccg
     1441 cgtagcgcgc gatttcaaga atcttggtgt gaattagctg gaaaggattc ggcgggttgc
     1501 tcggcagcaa cgaggaggac tcgccgaggg cgaatttcgt tgacgctcgt ttgcattgct
     1561 cattaacgtt ccgtggccgt tacaaaaccc tggggaaaac atgaaaatga aagtgaagaa
     1621 actgtccacc gcggtgattc acgcgctggg cgcgggagcc atgatcgcag cattcacgcc
     1681 ggccgcggtc gcgcagcagc cgccgccggc tccgaaggag cgcatcgaag tcaccgggtc
     1741 caacatcaag cgcgtcgagg gcgagagcgc gcttcccgtc accgtcatca cgcgcgagga
     1801 gatcgacaag tccggcgcga ccaacgccat ggagctgctg aacctcatcg gatccaacgc
     1861 gagcgccggt agcgtgctcg cggcgagcac catcggcgcc accacgttct cggcgcagac
     1921 cgccaacctg cgcggcctga ccggcgggcg cacgctggtt ctcatcaacg gaaagcgggt
     1981 caacggattc gcaggcgagg tgaacggcgt ccagggcgtg aacctctcgg tcatcccgtt
     2041 cgccgccatc gatcgcgtgg agatcctgaa ggacggcgcc tccgccgtgt acggctccga
     2101 tgcgatcgcg ggcgtgatca atttcatcat gcgctcggac tatc
//
LOCUS       DASVII010000458         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_57333, whole
            genome shotgun sequence.
ACCESSION   DASVII010000458 DASVII010000000
VERSION     DASVII010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57333"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2403)
                     /locus_tag="VF309_05985"
     CDS             complement(<1..>2403)
                     /locus_tag="VF309_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7466171.1"
                     /translation="EPVEPVEPVEPVEPVEPVEPVQRVEPRAPVPAAAVPAISAPSLQ
                     SIGEPAASSFAAEPATADLGEAQPGAEPELPESEKPFEAGKDQRKIKDDVDRDLLPVF
                     LDEAREILPAVSEGVRRWKGAPHDHGPAADLRRHLHTLKGSARMTGLMRLGELAHVLE
                     ARIIAMDEVENPPVQAFEEAEERIDRYSLSLERLGRGEDIVEAEPLHVSASAVFEQQK
                     DKPAALAMIAAAAQAVSQRDAAEPEGQATSRAALLRVNADMIDRFVNEAGELSIARSR
                     IEGEMAAFKRALNDLTDNIARMRAQLREIEIAAEGQMQSTIKLKEEHGEQFDPLEFDR
                     FSRMQELTRFLAESLSDVITVHQGLQKNIDETELAIHAQARLNRELQQGLMGVRLVPL
                     GNLADRFYRIVRQTAKELEKKANLELKGTRTELDRSVLEKITGPFEHLLRNSIAHGIE
                     KPEARVAAGKPEIGEISIDAVQRGNEVVLTVADDGAGLNFPRIREKAVAAGLLAPDIE
                     LPEAQLAQFIFMSGFSTATEITQISGRGVGMDVVKSEITALGGRVEITSAAGRGTTFT
                     ITLPLTLAVTQAVMLRAGPTLYAVPSVMIEQVQEFKAPVYAELVAKGEVLWKGNSYPL
                     RSLLPLLGEIDTATPLRQIPVLLLKSGLQRAAIRVDEIVGNREVVVKTIGPQLARLAG
                     ISGATVLGNGQVVLILNPVQLVHRAGGGTEPAPAAVQETPFMAESKSGNPLVMVVDDS
                     LTVRKITGRMLTREGYEVASAKDGVDALQQLQDLKPDLILLDVEMPRMDGFEFARNVR
                     ADE"
BASE COUNT          327 a          839 c          835 g          402 t
ORIGIN      
        1 ttcgtcggcg cgcacgttgc gcgcgaattc gaagccgtcc atgcgcggca tctcgacgtc
       61 gaggaggatc aggtcgggct tcaggtcctg cagctgctgc agggcgtcga cgccatcctt
      121 ggcgctcgcc acctcgtagc cctcgcgggt gagcatgcgc ccggtgatct tgcgcaccgt
      181 gagcgagtcg tcgaccacca tcaccagcgg attgcccgac ttcgactcgg ccatgaaggg
      241 cgtttcctgc accgccgccg gggcgggctc ggtcccgccg cccgcgcggt ggacgagctg
      301 caccgggttc aggatcaaca ccacctggcc gttgccgagc accgtggcgc cggagattcc
      361 cgcgagccgc gcgagctgcg gcccgatggt cttcacgacc acctcgcggt tgcccacgat
      421 ctcgtccacg cggatggcgg cgcgctgcaa gccgctcttc aggaggagca ccggtatctg
      481 ccgcagcggc gtcgccgtgt cgatctcgcc cagcagcggc aggagcgagc gcagcggata
      541 gctgttgccc ttccacagga cctcgccctt cgcgacgagc tccgcgtaga ccggggcctt
      601 gaattcctgg acctgctcga tcatgaccga cggcaccgcg tagagggtcg ggcccgcgcg
      661 cagcatcacc gcctgcgtca cggcgagggt caggggaagc gtgatggtga aggtcgttcc
      721 gcggcccgcg gccgaggtga tctcgacgcg cccgccgagg gcggtgatct cgctcttcac
      781 gacgtccatg ccgaccccgc gccccgaaat ctgcgtgatc tcggtggcgg tggagaagcc
      841 cgacatgaag atgaactgcg cgagctgcgc ctccggcagc tcgatgtcgg gggcgaggag
      901 acccgcggcg accgccttct cgcggatgcg cgggaaattc aggcccgcgc cgtcgtcggc
      961 caccgtgagc accacttcgt tgccgcgctg cacggcgtcg atggagatct cgccgatctc
     1021 gggcttgccc gccgcgacgc gcgcctcggg tttctcgatg ccgtgggcga tcgaattgcg
     1081 caacaagtgc tcgaagggcc cggtgatctt ctcgagcacc gagcggtcga gctcggtgcg
     1141 cgtgcccttc agctcgaggt tcgccttctt ctcgagctcc ttcgcggtct ggcgcacgat
     1201 gcggtagaag cggtcggcga ggttgccgag cggcacgagg cgcacgccca tcaacccctg
     1261 ctgcagctcg cggttgagcc gcgcctgcgc atggatcgcg agctcggtct cgtcgatgtt
     1321 cttctgcagg ccctggtgca ccgtgatcac gtcggagagc gactccgcga ggaagcgcgt
     1381 gagctcctgc atgcgcgaga agcggtcgaa ctcgagcggg tcgaactgct cgccatgctc
     1441 ctccttcagc ttgatcgtgc tctgcatctg gccttcggcc gcgatctcga tctcgcgcag
     1501 ctgcgcgcgc atgcgcgcga tgttgtccgt gaggtcgttc agcgcgcgct tgaacgccgc
     1561 catctcgccc tcgatgcgcg agcgcgcgat ggaaagctcg ccggcctcgt tcacgaagcg
     1621 gtcgatcatg tcggcgttca cgcgcaagag cgccgcccgg ctcgtcgcct gcccctccgg
     1681 ctcggccgcg tcgcgctggg agaccgcctg cgctgcggcg gcgatcatcg cgagcgcggc
     1741 gggcttgtcc ttctgctgct cgaacaccgc cgacgccgaa acgtggagcg gctcggcctc
     1801 gacgatgtcc tcgccgcggc cgaggcgctc gagcgatagc gagtagcggt cgatgcgctc
     1861 ctcggcttct tcgaaggcct ggaccggcgg attctcgact tcgtccatcg cgatgatccg
     1921 cgcctcgagc acgtgggcga gctcgcccag gcgcatgaga cccgtcatgc gcgcgctgcc
     1981 cttcagcgtg tgcaggtggc ggcgcagatc cgcggcgggg ccgtgatcgt gcggcgcgcc
     2041 cttccagcgg cgcacgcctt cgctgacggc cggaaggatt tcccgcgcct cgtcgagaaa
     2101 gaccggcagg aggtcgcgat cgacgtcgtc cttgatcttg cgctggtcct ttccggcttc
     2161 gaacggcttt tcggactcgg gcagctcggg ctcggcccca ggctgggcct cgccgagatc
     2221 cgcggtggcg ggttcggccg cgaacgacga cgcagcgggc tcgccgatcg attgcaacga
     2281 gggtgccgag atggcgggca ccgccgccgc cggcaccgga gcccgaggct cgacacgctg
     2341 gacgggctcg acgggctcga ccggctcgac gggctccacg ggctcgacgg gctcgacggg
     2401 ctc
//
LOCUS       DASVII010000459         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_560891,
            whole genome shotgun sequence.
ACCESSION   DASVII010000459 DASVII010000000
VERSION     DASVII010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560891"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..620)
                     /locus_tag="VF309_05990"
     CDS             complement(93..620)
                     /locus_tag="VF309_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX7466172.1"
                     /translation="MKSQLVLLSLGAVLAAGCSMQHMKHMDPTAAFANLEPTKGNSAR
                     GTVSFTQDGDEVRVRATISGLKPNAEHGFHVHEKGDCSSGDGMSAGGHFNPSGKPHGP
                     QSAEHHAGDMPSLRSDAYGNASASFELKGVSIGGANADLVGRGLILHRDPDDYATQPT
                     GNAGPRIACGVIQKS"
     gene            complement(642..1037)
                     /locus_tag="VF309_05995"
     CDS             complement(642..1037)
                     /locus_tag="VF309_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466173.1"
                     /translation="MKRMTIAGALLAFTLGTGAAWAKIPPPPPPDEKAKAAAEDKKAK
                     DAVAAEKGKADQAAAEDKAVKNFQGNMKKMGKPIPKPTPVAAVTPPQLMPAPAKAGTS
                     SQGHEKGAMTPAGNTPPKGNDTPVDAKKK"
     gene            complement(1041..>1972)
                     /locus_tag="VF309_06000"
     CDS             complement(1041..>1972)
                     /locus_tag="VF309_06000"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX7466174.1"
                     /translation="STAKGSTAIPGWNNPPASWDSTSELPQYASIPGRETNRLIQGTG
                     REWRVFHNGPLVQYGGWLIVIVLAAIAVFYLIKGTIKLKGRPTGRLIERFNAVERASH
                     WTMAGSFVFLALTGVTMLFGKFLILPWLGYGAFSWLTIVGKNIHNFVGPLFIFSLVVS
                     FLIFVKDNMIHKVDIEWLTKMGGMFSGHEVASGRFNAGEKAWFWGGLVLLGALVSITG
                     LILDFPNWNQGRELMQQANVVHAVCAVLFISAAFAHIYIGTLGMEGAYRAMRDGYVDE
                     EWAKEHHALWYQEVKDGKRPEKIAGAPQPATGD"
BASE COUNT          295 a          650 c          660 g          367 t
ORIGIN      
        1 atgcacgcac gatcgtcttt cccgtgcgag tggccgcgaa agcgcaggcg accgtgcgct
       61 acacggtgcg ctacacctgg tgaagcggcg tgtcaggact tctggatcac tccacaggcg
      121 atgcgcggac ccgcgttgcc ggtgggctgc gtggcgtagt cgtcgggatc ccgatgcagg
      181 ataaggccgc ggccgacgag atccgcgttg gcgccgccga tggaaacgcc tttcagctcg
      241 aacgatgccg aagcgttgcc ataggcgtcg gacctgaggc tcggcatgtc gccggcgtgg
      301 tgctccgcgg attgcgggcc gtggggcttg cccgacggat tgaagtggcc gccggcgctc
      361 atgccgtcgc ccgagctgca gtcgcccttc tcgtgcacgt ggaagccgtg ctcggcgttc
      421 ggcttgagcc ccgaaatcgt cgcgcgcacg cgcacctcgt ctccgtcctg cgtgaaggaa
      481 accgtgccgc gggccgagtt gcccttggtg ggctcgagat tcgcgaaggc cgcggtcggg
      541 tccatgtgct tcatgtgctg catcgagcaa cccgcggcga gaaccgcgcc gagcgataac
      601 aggacgagct ggctcttcat cgcgatctcc gtttcacgtc gtcacttctt cttggcatcc
      661 accggcgtat cgttgccctt gggcggcgtg ttgcctgcgg gcgtcatcgc gcccttctcg
      721 tggccctgcg acgacgttcc ggccttcgcg ggagcgggca tcaactgcgg cggggtcacg
      781 gcggcgaccg gcgtcggctt cgggatgggc ttgcccatct tcttcatgtt cccctggaaa
      841 ttcttcacgg ccttgtcctc ggccgcggcc tggtcggctt tgcccttctc ggcggcgacg
      901 gcgtccttcg ccttcttgtc ctcggcggcg gccttggcct tctcgtctgg cggcggaggc
      961 ggaggaatct tcgcccatgc cgcgcccgtg ccgagcgtga atgcgagcag cgccccagcg
     1021 atcgtcatgc gcttcatggc tcagtctccc gtggccggtt gcggcgcgcc cgcgatcttc
     1081 tcgggacgct tgccatcctt cacctcctgg taccagaggg cgtgatgctc cttggcccac
     1141 tcctcgtcga cgtagccgtc gcgcatggcg cgataggcgc cttccattcc gagcgtgccg
     1201 atgtagatgt gcgcgaacgc ggccgagatg aagagcaccg cgcagaccgc gtgcaccacg
     1261 ttcgcctgct gcatgagctc gcgcccctgg ttccagttgg ggaagtcgag gatgagcccg
     1321 gtaatgctca cgagcgctcc gaggagcacg agcccgcccc agaaccacgc cttctcgccc
     1381 gcgttgaagc gccccgaggc gacctcatgg ccgctaaaca tcccgcccat cttggtgagc
     1441 cattcgatgt cgaccttgtg gatcatgttg tccttcacga agatgaggaa ggacaccacc
     1501 agggagaaga tgaagagcgg ccccacgaag ttgtggatgt tcttcccgac gatcgtgagc
     1561 caggagaacg cgccgtagcc gagccacggc aggatgagga acttgccgaa cagcatggtg
     1621 accccggtga gcgcgaggaa gacgaagctc cccgccatgg tccagtggga cgcgcgctcg
     1681 accgcgttga agcgctcgat gaggcggccc gtcgggcggc ccttgagctt gatcgttccc
     1741 ttgatgaggt agaacaccgc gatcgccgcg agcacgatga cgatgagcca gccgccgtac
     1801 tgcacgagcg gcccgttgtg gaacacgcgc cattcgcgcc cggtgccctg gatgaggcga
     1861 ttggtctcgc gcccgggaat cgaggcgtac tgcggcagct cgctggtgct gtcccacgac
     1921 gccgggggat tgttccaccc cggaatcgcc gtcgagccct tcgcggtgga ga
//
LOCUS       DASVII010000460         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_57480, whole
            genome shotgun sequence.
ACCESSION   DASVII010000460 DASVII010000000
VERSION     DASVII010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57480"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VF309_06005"
     CDS             complement(<1..889)
                     /locus_tag="VF309_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7466175.1"
                     /translation="MNALIALVRKDLLLYMSNRRALAITLVAPILIAAFFGAVLGSPP
                     KKPAKIPIAVVDLDASALTASIVASLKADALFDLHELTQPEAVSEVRNGKTRVAIVFP
                     RGFGDAAPRALFRPGPKPEIALHVDPSQTMVLAMVKGILTQYVMQAVSRVAFGGGGEA
                     GVKVLADARSEVERSTALDPAERRDLLALFQSVERVNLRRAGATSATGGAGLAMQTPF
                     VTAEHEVTSGERRYNAYAHSFAGMTVQFVLFLGIEMGVAVLYMRRLGLWKRLRAAPVS
                     RALLLGSRIASGTLIATILT"
     gene            complement(892..>1559)
                     /locus_tag="VF309_06010"
     CDS             complement(892..>1559)
                     /locus_tag="VF309_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466176.1"
                     /translation="GVVRVGGVALDGDAGTQKRRLGLVPQEIALHEDLRAIENVRLFG
                     ALYGLKGRDLEERARAILDQVGLGDRARDKPETFSGGMKRRLNIACALVHDPEVLLLD
                     EPTAGVDPQSRNAIFESLERLRARGKAILYTTHYMEEAERLCDRIVIIDHGKVIAAGS
                     TAELHRLAPRPAATLLEIEGAVDEATLAALAAQGVVAREVRSKGASLEAVFLHLTGRD
                     LRD"
BASE COUNT          227 a          541 c          554 g          237 t
ORIGIN      
        1 tcgtgaggat cgtggcgatc agcgtgcccg aggcgatgcg gctgccgagg aggagcgcgc
       61 gcgacacggg tgccgcacgc agccgcttcc acaatccgag gcggcgcatg tagagcacgg
      121 ccacgcccat ctcgatcccc aggaagagga cgaactgcac cgtcatgccg gcgaaggagt
      181 gcgcgtaggc gttgtagcgc cgctcgcccg acgtgacttc gtgctccgcg gtgacgaacg
      241 gcgtctgcat cgcgaggccc gcgccgcccg tcgccgaagt cgcgcccgcg cgccgcaggt
      301 tcacgcgctc cacgctctgg aagagcgcga gcaggtcgcg gcgctcggcc ggatcgagcg
      361 cggtgctcct ttccacctcc gagcgtgcat ccgcgagcac cttcacgccg gcttccccgc
      421 cgccgccgaa ggccacgcgc gacaccgctt gcatcacgta ctgcgtgagg atgcccttca
      481 ccatcgcgag caccatcgtt tgcgacggat cgacgtgcag cgcgatctcg ggcttcggtc
      541 cgggccggaa cagggcgcgc ggcgccgcat ccccgaaacc gcgcgggaac acgatcgcca
      601 cgcgcgtctt gccattgcgc acctcgctca ctgcctcggg ctgggtgagc tcatgcaagt
      661 cgaacagcgc gtcggctttg agcgatgcga cgatcgatgc ggtgagcgcg cttgcgtcga
      721 ggtcgacgac ggcgatcggg atcttcgccg gcttcttcgg cgggcttccc aggaccgcgc
      781 cgaagaaggc ggcgatgagg atcggcgcga ccagagtgat ggcgagcgcg cggcgattgg
      841 acatgtagag gaggaggtcc ttgcgtacca gggcgatgag cgcgttcatg gtcagtccct
      901 caggtcgcgg ccggtgagat gcaggaacac cgcctcgagg gacgcgccct tcgagcggac
      961 ttcccgtgcc accacgcctt gcgcggcgag cgcggcgagc gtggcttcgt ccacggcgcc
     1021 ttcgatctcg aggagggtcg cggccgggcg cggcgccagg cggtgcagct cggcggtgct
     1081 gccggcggcg atcaccttgc cgtggtcgat gatcacgatg cggtcgcaca gccgctcggc
     1141 ctcttccatg tagtgcgtcg tgtagaggat cgccttgccg cgcgcgcgca ggcgctcgag
     1201 gctctcgaag atcgcgttgc ggctttgcgg atcgacgccg gcggtgggct cgtcgaggag
     1261 cagcacttcg ggatcgtgca ccagcgcgca cgcgatgttg aggcgccgct tcatgccgcc
     1321 gctgaaggtc tcgggcttgt cgcgcgcgcg atcgccgagc cccacctgat cgaggatggc
     1381 gcgcgcgcgc tcctcgaggt cgcggccctt caggccgtac aacgcgccaa aaaggcgcac
     1441 gttctcgatc gcccgcaagt cctcgtgcag cgcgatttcc tggggcacga gcccgaggcg
     1501 gcgcttctgg gtgccggcgt cgccgtcgag ggccaccccg cccacgcgca ccacgcccc
//
LOCUS       DASVII010000461         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_273075,
            whole genome shotgun sequence.
ACCESSION   DASVII010000461 DASVII010000000
VERSION     DASVII010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273075"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VF309_06015"
     CDS             <1..559
                     /locus_tag="VF309_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX7466177.1"
                     /translation="LSEAEVEALLGAPDVETAQGLRDRAMLETLYASGLRVSELVGLK
                     TVQVSLDMNVIRILGKGAKERLTPLGEEASDWIVRYQREARQQLLRARKSDALFVTAR
                     GGPMTRQSFWSLVKRHARTAGIGQAISPHTLRHAFATHLINHGADLRVVQMLLGHADI
                     STTQIYTHVARERLKALHRKHHPRG"
     gene            560..1312
                     /locus_tag="VF309_06020"
     CDS             560..1312
                     /locus_tag="VF309_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7466178.1"
                     /translation="MEINDLSPWLLLVAPAIIVVGYTVFGLSGFGSTVISVPVLAHFL
                     PVSYLVPLMALLALASASLVGSASREHVSKPEMKRIIPWMFVGFVVGVTLLKGMPDEY
                     LRMALGIFAMSVGAYSIFNPTVIRTFSAWWSVPAGIVGGAIATIFGAGGPIYSTYLAG
                     RLKDKSEIRATISTLISISAFTRAIMYAVAGLLLHVPIFAGFVALAPFVWVGLKVGDR
                     IHVGLSQEQMRRAIGCVLVLTGGSLLVRVFFA"
     gene            1318..>1790
                     /locus_tag="VF309_06025"
     CDS             1318..>1790
                     /locus_tag="VF309_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEX7466179.1"
                     /translation="MKSSATPATVFIAKHGVAFTEHHFEYVEHGGTAHTSSALGVPEH
                     QVVKTLIMEDEKGEPLVVLMHGDRKVSTKELARAAGVKRIAPCKPETATRHSGYLIGG
                     TSPFGARKSMPVYLERSVLDLPRIYINGGRRGYLLGMDSSELVRLLSPVLVDVAL"
BASE COUNT          270 a          614 c          586 g          320 t
ORIGIN      
        1 gctctcggaa gccgaggtcg aggcgctgct cggagctccc gatgtagaga ccgcgcaggg
       61 cctgcgcgat cgcgcgatgc tcgagaccct ctatgcgagc gggctgcgcg tctccgagct
      121 ggtggggttg aagacggtgc aggtgagcct cgacatgaac gtgatccgca tcctcggcaa
      181 gggcgcgaag gagcgcctca cgccgctggg tgaagaggct tccgactgga tcgtgcgcta
      241 ccagcgggag gcgcggcagc agctgctgcg cgcacgtaaa tccgacgcgc tcttcgtcac
      301 ggcgcgcggc ggcccgatga cgcgccagtc gttctggtcc ctcgtcaagc gacacgcgcg
      361 taccgcgggc atcgggcagg cgatctcccc gcacacgctg cgccacgcgt tcgcgacgca
      421 cctcatcaac cacggcgccg accttcgcgt agtccagatg ctcctcgggc acgccgacat
      481 ttcaaccacg cagatctaca cccacgtcgc ccgcgagcgg ctgaaggccc tgcatcgcaa
      541 gcaccacccg cgcggataaa tggaaatcaa cgatctctcg ccgtggctgc tgctggtggc
      601 gcccgccatc atcgtcgtcg ggtacaccgt gttcgggttg agcggcttcg gctcgaccgt
      661 catctcggtc ccggtgctcg cccatttcct gccggtctcc tatctcgtgc cgttgatggc
      721 gctcctcgcc ctcgcctcgg cttccctcgt cggctccgcc agccgcgagc acgtctcgaa
      781 gcccgagatg aagcgcatca ttccgtggat gttcgtgggg ttcgtggtgg gcgtcacgct
      841 cctgaagggg atgcccgatg agtacctgcg catggcgctc ggcatcttcg cgatgagcgt
      901 gggcgcgtac agcatcttca atcccacggt gatccgcact ttctcggcat ggtggtccgt
      961 gcccgccggg atcgtgggcg gcgcgatcgc gaccatcttc ggcgcgggcg gcccgatcta
     1021 ctcgacgtac ctggcgggaa ggctcaagga caagagcgag atccgcgcga cgatctccac
     1081 gctcatctcc atctcggcct tcacgcgggc gatcatgtac gcggtcgctg gcctcctgct
     1141 gcacgtgccg atcttcgcgg gattcgtggc gctcgcgccc ttcgtgtggg tggggctcaa
     1201 ggtcggcgat cgcatccacg tggggctctc gcaggagcag atgcgccgcg ccatcggctg
     1261 cgtgctggtg ctcaccgggg gcagcctcct ggtgcgcgtg ttcttcgcct gactgccatg
     1321 aaatcgtccg ccactcccgc caccgtgttc atcgcgaagc acggcgtcgc cttcaccgag
     1381 catcacttcg agtacgtcga gcacggcggc acggcccaca cctcgagcgc gctcggcgtg
     1441 cccgagcatc aagttgtcaa gacgctcatc atggaagacg agaagggcga gccgctcgtg
     1501 gtcctcatgc acggcgatcg caaggtctcg accaaggagc tcgcgcgggc ggcgggcgtg
     1561 aagaggatcg cgccctgcaa gcccgagacc gcgacgcgac actcgggata cctcatcggc
     1621 gggacatcgc ccttcggcgc gcgcaagagc atgccggttt acctcgagcg ctcggtgctc
     1681 gaccttcccc ggatctacat caacggcggg cggcgcgggt acctcctcgg catggattcg
     1741 tcggagctgg tgcggctact ctcgcccgtg cttgttgacg tggcgctgga
//
LOCUS       DASVII010000462         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_488632,
            whole genome shotgun sequence.
ACCESSION   DASVII010000462 DASVII010000000
VERSION     DASVII010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488632"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VF309_06030"
     CDS             complement(<1..1019)
                     /locus_tag="VF309_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7466180.1"
                     /translation="MSETPHRGALSDALVLFGVTGNLAHKKIFPALYAMAKKGALDRP
                     VVGVASSKWTVAQLREHAEAGIRESCKIDDRAALRHVLSLLRYVQGDYNDPATFKALK
                     AALAAARRPAFYLAIPPALFPTVIKGLGASGLAAGARVIVEKPFGRDLASARELNRIA
                     LSVFPEDSIFRIDHFLAKEAIMNILYFRFANSFLEPIWNRNYVASVQVTLAESFGVEG
                     RGAFYETAGCMRDVIQNHLFQIVALLAMEPPTYRGFGAVHGETADILQAMRPLVPDDV
                     VRGQYTGYRKEPGVAKRSDVETYAALKLWIDSWRWGGVPFYIRAGKMLPTTCTEVIVQ
                     LKAPP"
     gene            complement(1037..1807)
                     /locus_tag="VF309_06035"
     CDS             complement(1037..1807)
                     /locus_tag="VF309_06035"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX7466181.1"
                     /translation="MTTNDPPAIALAYKDAEFLEGDDARPLRILAEYLAPMHAFKRER
                     IRDTVVFFGSARLKEDGPLGEYYAAARELARLVTAWSKGLPSHAHRYVVCTGGGGGLM
                     EAANRGATDAGGKTIGLNIGLPHEQRPNPYVTPGLSFEFHYFFMRKLWFSLLARALVV
                     FPGGFGTLDEMFEVLTLMQTQKLNRRITVVLYGSSYWKELVDFEALARHGMIEREALP
                     LFRYADDPATALALLQAGIAAELEEATPAIAHSRTSCN"
     gene            complement(1804..>2336)
                     /locus_tag="VF309_06040"
     CDS             complement(1804..>2336)
                     /locus_tag="VF309_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX7466182.1"
                     /translation="ADSARFPDLSLSIIKLLGSGEYALEIPGQPAEGHFGLAVKDYTH
                     STAPNRRFPDVITQRLLKAALEARAQPYTSDELKALALHCTAQEDNAAKVERRVGKSA
                     AALLLESRIGAQFDGIVTGASDKGTWVRITAPAAEGRVVRGFEGLQVGNRVRVQLVHT
                     DAARGFIDFASARGAS"
BASE COUNT          380 a          769 c          805 g          382 t
ORIGIN      
        1 tgcggcggtg ccttcagctg cacgatcacc tcggtgcagg tggtcggaag catcttgccg
       61 gcgcggatgt agaacggcac cccgccccag cgccagctgt cgatccagag cttcagcgcg
      121 gcgtaggtct cgacgtcgga gcgcttcgcg acaccgggct ccttccgata gcccgtgtat
      181 tgcccgcgca ccacgtcgtc gggaacgagc gggcgcatcg cctgcaggat gtcggccgtt
      241 tcgccatgca cggcgccgaa gcctcgatac gtcggcggct ccatcgcgag gagcgccacg
      301 atctggaaaa ggtggttctg gatcacgtcg cgcatgcatc cggcggtctc gtagaacgcg
      361 ccgcggccct cgacgccgaa gctctccgcg agcgtcacct gcacgctcgc cacgtagttg
      421 cggttccaga tcggctcgag gaacgagttg gcgaagcgga aatagaggat gttcatgatc
      481 gcttccttgg cgaggaagtg atcgatgcgg aagatcgagt cttccgggaa gaccgagagc
      541 gcgatgcggt tcagctcgcg cgcggacgcg aggtcgcgcc cgaagggctt ctccacgatg
      601 acgcgcgccc cggcagcgag gcccgaagcg ccgagcccct tgatgacggt cgggaatagc
      661 gccggtggaa tcgcgagata gaaagccggg cgccgcgcgg cggcgagggc cgccttgagc
      721 gccttgaacg tcgccggatc gttgtagtcg ccctggacgt agcgcaggag cgagagcacg
      781 tggcgcagcg ccgcgcggtc gtcgatcttg caggattcgc ggatgccggc ctcggcgtgc
      841 tcacgcagtt gcgcgacggt ccacttcgag gaagccacgc cgacgaccgg gcgatcgagg
      901 gcgcccttct tcgccatcgc atagagcgcc gggaagatct tcttgtgcgc gagattgccg
      961 gtcacgccga acaacaccag cgcgtccgac aatgcgccgc ggtggggggt ctcgctcatc
     1021 gtcgcgctgg atttcttcag ttgcacgatg ttcgcgagtg cgcgatcgcc ggcgtcgctt
     1081 cctcgagctc cgccgcgatt cccgcctgca gcaatgccag cgccgtcgcc ggatcgtcgg
     1141 cgtacctgaa gagcgggagg gcctcgcgct cgatcatgcc gtggcgcgcg agcgcctcga
     1201 aatcgacgag ctccttccag tagctcgatc cgtaaagcac gaccgtgatg cgccggttga
     1261 gcttctgcgt ctgcatcagg gtcaggacct cgaacatctc gtcgagcgtt ccgaatcctc
     1321 ccgggaacac gacgagcgcg cgggcgagca acgagaacca gagcttgcgc atgaagaaat
     1381 agtggaactc gaacgagagc cccggcgtga cgtaggggtt gggccgctgc tcgtggggca
     1441 ggccgatgtt gaggccgatg gtctttccgc ccgcgtccgt cgcgccgcgg ttggcggcct
     1501 ccatgaggcc gccgcccccg ccggtgcaga cgacgtagcg atgcgcgtgc gaaggcaggc
     1561 ctttcgacca cgcggtgacc aggcgggcga gctcgcgcgc cgcggcgtag tactcgccta
     1621 gcgggccatc ctccttcagc cgcgccgatc cgaagaacac gacggtgtcg cgaatgcgct
     1681 cgcgcttgaa ggcgtgcatc ggcgcgagat attccgcgag gatgcgcagc ggcctcgcgt
     1741 cgtcgccctc gaggaactcc gcatccttgt aggcgagcgc aatggcgggc ggatcgttcg
     1801 ttgtcacgat gcccctctcg cggaagcgaa gtcgatgaag ccgcgcgcgg cgtcggtgtg
     1861 gaccagttgc acgcgaacgc ggttgccgac ctgcaatccc tcgaagcccc gcacgacgcg
     1921 cccttcggcg gcgggcgcgg tgatgcgcac ccacgttccc ttgtccgatg cgccggtcac
     1981 gatgccgtcg aactgcgcgc cgatccgcga ttcgagaagc agcgccgccg cggacttccc
     2041 gacccggcgc tccaccttgg cggcgttgtc ctcctgcgcg gtgcagtgaa gcgcgagcgc
     2101 cttcagctcg tccgacgtgt agggctgcgc ccgcgcctcg agcgccgcct tcagcaggcg
     2161 ctgcgtgatg acgtccggaa agcggcgatt cggcgccgtc gaatgggtgt aatccttcac
     2221 ggcaaggccg aagtggcctt cggccggttg tcccggtatc tcgagcgcgt actcgccgga
     2281 acccagcagc ttgatgatcg agagcgaaag atcgggaaag cgcgcggaat ccgcct
//
LOCUS       DASVII010000463         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_273098,
            whole genome shotgun sequence.
ACCESSION   DASVII010000463 DASVII010000000
VERSION     DASVII010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273098"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /gene="folK"
                     /locus_tag="VF309_06045"
     CDS             complement(<1..274)
                     /gene="folK"
                     /locus_tag="VF309_06045"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7466183.1"
                     /translation="MSETVRAAIALGSNLDDPEAHVRRGFDDLAALARTKVLARSKLY
                     RTAPVGYADQPDFVNACALVETSLAPRALLDELLAIEARHGRVRTIP"
     gene            complement(271..1638)
                     /gene="pcnB"
                     /locus_tag="VF309_06050"
     CDS             complement(271..1638)
                     /gene="pcnB"
                     /locus_tag="VF309_06050"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX7466184.1"
                     /translation="MIKKYIDKLFGRRERVTHKDARPTVYGKDKHNIQRELISRCARR
                     TCEDLQRAGHATFVVGGAVRDLLLGRRPKDFDIATSATPDEVRSLFRRSRIIGRRFQI
                     VHVMCGAETVEVSTFRAHFTREVTEENTDEHGRMLSDNVFGTQAEDAMRRDFTVNALF
                     YDPVKEEVWDYVHGLKDLKSKKLVMIGDPATRYREDPVRMLRAARLGAKLGLEIDAKT
                     KEPIREMRHLLENVPQARLFEEILKLLLSGNAVECVRVLRELNLHHGLLPLLDTSLDD
                     PETGPFAMAALRATDERLAADKPVSPAFLLAALLWGRVEKSLKKYEERGEPAVPALHS
                     AMHEALDAQRDSLAIPRRFDATMKELWLMQPRFLQRGGQRPYRLLEHPRFRAAYDFFA
                     LRAASANAPQEAAQWWERFQEASADEREEMLVSGESGPKKKRRRRRGKRREDAPPTPH
                     DEGQS"
     gene            complement(1635..2294)
                     /locus_tag="VF309_06055"
     CDS             complement(1635..2294)
                     /locus_tag="VF309_06055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7466185.1"
                     /translation="MKLALFDLDNTLLDGDSDHAWAQFLIEEGIVHAGEYHAKNEWFY
                     ERYKDGTLDIHEFLDFQLAPIARRPRSQLDAWHNEFMQRKVRPMILPKAPELIARHAD
                     AATAIVTATNRFITAPIAAELGVAKLIATDIEEVDGVFTGKPRGTPAFREGKVVLVDE
                     WLGESGQRLADCESWFYSDSLNDLPLLERVTHPVAVDPDATLRACALERGWPIISLRA
                     A"
     gene            complement(2291..>2817)
                     /locus_tag="VF309_06060"
     CDS             complement(2291..>2817)
                     /locus_tag="VF309_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX7466186.1"
                     /translation="ERVMYVWGESGAGKTHLLKAFAQASGARARYVPARDFMDAAGAP
                     VFAVDDLEQLTEEEQSSLFNAFNERAFEFLLVAASSAPRDVAIRRDLATRLATGLTYR
                     VVALSDDEKRAALAAHAKARGFTIPGEVTAYLLTHARRDMGSLIAALDSIDRYSLETG
                     RPVTVPLLKAALQG"
BASE COUNT          401 a          958 c         1001 g          457 t
ORIGIN      
        1 tgggaattgt gcgcacgcgg ccgtggcgcg cctcgatcgc gagcagctcg tcgagcagcg
       61 cgcgcggcgc gagggacgtc tcgaccagcg cgcatgcgtt cacgaaatcg ggctggtcgg
      121 cgtaaccgac gggcgccgtg cgataaagct tcgagcgcgc caggaccttc gtccgcgcca
      181 gcgccgcgag atcgtcgaag ccgcggcgca cgtgcgcctc ggggtcgtcg aggttggatc
      241 ccagcgcgat cgcggcgcgc accgtctcgc tcacgattgc ccttcgtcgt gaggcgtggg
      301 cggcgcatcc tccctgcgct tgccgcggcg gcggcggcgt ttcttcttcg ggcccgattc
      361 cccggagacg agcatctcct cgcgctcgtc ggcgctcgct tcctggaagc gctcccacca
      421 ctgcgcggct tcctgcggcg cgttggccga ggccgcgcgc agcgcgaaga aatcgtacgc
      481 ggcgcggaag cgcgggtgct cgaggaggcg atagggacgc tgcccgccgc gctgcaggaa
      541 gcgcggctgc atgagccaga gctccttcat cgtcgcatcg aagcggcgcg ggatcgcgag
      601 cgagtcgcgc tgggcgtcga gcgcctcgtg catcgccgag tgcagcgcgg gaaccgcggg
      661 ctcgcccctc tcctcgtact tcttcaggct cttctcgacg cgcccccaca gcaacgccgc
      721 gaggagaaac gcgggcgaga cgggcttgtc ggccgcgagc cgctcgtcgg tggcgcgcag
      781 cgccgccatc gcgaagggcc cggtctcggg atcgtcgagc gaagtgtcga ggagcggcag
      841 caggccgtga tgcaggttca gctcgcgcag cacgcgaacg cactcgacgg cgttgcccga
      901 cagcagcagc ttcaggatct cctcgaagag ccgcgcctgc gggacgttct cgagaaggtg
      961 gcgcatctcg cggatcggct ccttcgtctt cgcgtcgatc tcgaggccca gcttcgcgcc
     1021 gaggcgcgcc gctcgcagca tccgcaccgg gtcctcgcgg tagcgcgtcg ccggatcgcc
     1081 gatcatcacg agcttcttcg acttcaggtc cttcagcccg tgcacgtagt cccagacctc
     1141 ttccttcacc gggtcgtaga agagcgcgtt cacggtgaag tcgcggcgca tggcgtcctc
     1201 ggcctgggtg ccgaagacgt tgtccgagag catgcggccg tgctcgtcgg tgttctcctc
     1261 ggtcacctcg cgggtgaagt gcgcccggaa ggtcgagacc tcgaccgtct ccgcgccgca
     1321 catcacgtgc acgatctgga agcgccggcc gatgattcgc gagcggcgga agagcgagcg
     1381 cacctcgtcg ggcgtggcgc tggtggcgat gtcgaaatcc ttgggccggc gcccgagcag
     1441 gaggtcgcgc acggcgcccc ccaccacgaa ggtcgcatgg cccgcgcgct gcaggtcctc
     1501 gcaggtgcgc cgcgcgcagc ggctgatgag ctcgcgctgg atgttgtgct tgtccttgcc
     1561 gtagacggtc gggcgcgcgt ccttgtgcgt cacgcgctcg cggcggccga acagcttgtc
     1621 gatgtatttc ttgatcacgc agccctcaat gagatgatcg gccagccccg ctccagcgcg
     1681 caggcccgca gggtggcatc gggatcgacc gccaccgggt gagtcactcg ctcgagaagc
     1741 ggcaggtcgt tcagcgaatc gctgtagaac cagctctcgc agtccgcgag gcgctggccg
     1801 ctctcgccga gccattcgtc tacgagcacg accttcccct ccctgaacgc cggcgtgccg
     1861 cgcggcttcc cggtgaagac gccgtcgacc tcctcgatgt cggtcgcgat gagcttggcc
     1921 acgccgagct ccgccgcgat gggcgcggtg atgaagcggt tggtggccgt gacgatcgcc
     1981 gtcgccgcgt cggcgtgccg cgcgatcagc tcgggggcct tgggaaggat catcgggcgc
     2041 accttgcgct gcatgaactc gttgtgccag gcatcgagct gcgagcgcgg gcgccgcgcg
     2101 atgggggcga gctggaaatc gaggaactcg tggatgtcga gcgtgccgtc cttgtagcgc
     2161 tcgtagaacc attcgttctt cgcatggtac tcgccggcat ggacgatgcc ttcctcgatg
     2221 aggaattgcg cccacgcgtg gtcgctgtcg ccgtcgagga gcgtgttgtc gaggtcgaag
     2281 agcgcgagct tcatccctgc agcgccgcct tgagcagcgg cacggtcacg ggccgcccgg
     2341 tctcgagcga gtagcgatcg atcgaatcga gcgcggcgat gagcgagccc atgtcgcggc
     2401 gcgcgtgggt gaggaggtaa gccgtcacct cgccgggaat ggtgaagccg cgggccttcg
     2461 cgtgggcggc gagcgcggcg cgcttctcgt cgtcgctaag cgccacgacg cgataggtga
     2521 gccccgtcgc aaggcgcgtc gccaggtcgc gccggatggc cacgtcgcgc ggcgccgagc
     2581 tcgccgcgac caggaggaat tcgaaggcgc gctcgttgaa cgcgttgaag agcgacgact
     2641 gctcctcctc ggtgagctgc tcgaggtcgt ctacggcgaa caccggcgcc cccgccgcat
     2701 ccatgaagtc gcgggccggg acgtagcgcg cgcgcgcgcc gctcgcctgc gcgaaggcct
     2761 tcagcagatg ggtcttgccg gcgccggatt cgccccacac gtacatcaca cgctcgc
//
LOCUS       DASVII010000464         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_129484,
            whole genome shotgun sequence.
ACCESSION   DASVII010000464 DASVII010000000
VERSION     DASVII010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129484"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..955)
                     /locus_tag="VF309_06065"
     CDS             complement(215..955)
                     /locus_tag="VF309_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7466187.1"
                     /translation="MHAAQGSDGAETIDALRRDVALANRILERMGLAHAFGHASARIP
                     GTNTFLIPTRRSPGLAEAGNLLVIDTEGRLVSGEGEPNSEFWIHARIYAARAEAGGVV
                     HAHPPSCVCLTQIGEPHRVVHNQGGIFAGGVPEYSRVGLIRTRELGDLLAQVVGKGNS
                     AMMRGHGITTVFPDVRAATVAACYLEESATLQLRMLAAAGGDASRIRAFTREEADGLR
                     DQITGNVAKRAWEYLAAVALENPLGSGR"
     gene            complement(955..2178)
                     /locus_tag="VF309_06070"
     CDS             complement(955..2178)
                     /locus_tag="VF309_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7466188.1"
                     /translation="MSRILDAVRVLDLTRVLAGPWSTQNLADLGAEVIKVERPGLGDD
                     TRQWGPPFLKDREGRDTRDAAYFLACNRGKKSLTLDLASPAGQSVARSLAAKSDVLVE
                     NFKVGDLARYGLDYASLASDNPGLVYCSITGFGQDGPYRDRPGYDFMVQGLGGLMSVT
                     GERDDLPGGGPQKVGVAVSDLFTGMYATCAILAALLHRDRTGRGQYIDMALLDVQVAM
                     LANLSSAYFCSNEVPQRMGNAHQAIVPYHVFRASDEFLVIAVGNDTQFAKFCEVIGAP
                     EWPLDARFTTNPERVRHRDLLVGMIAARMRSRSAREWLAALEPSGVPCGPINDLSQVF
                     ADPQVRHRGMQVAAPHPVAGEMKMVANPMKFSATPIVHDRPAPLLGEHTDDVLRGVLG
                     MGDADIAQLRANGVI"
BASE COUNT          368 a          778 c          836 g          384 t
ORIGIN      
        1 atcggtgggt cgcaactctt cttcctcctc gctcgtcgct gagcgaatcc ctctcaccgg
       61 ggaatcgtga gccagtctac cgaataaacc gttgacggga caagggaaac atcacctttt
      121 gcttatattc gaggggtttc ggcgggaggc cttgaatgcg cccgcaagac gccagggaac
      181 acggcaaccc gcgggctcgg gccgatacgc tgcatcagcg ccccgagccg agcggatttt
      241 cgagggcgac ggcggcgagg tattcccacg cgcgcttggc gacgttgccg gtgatctggt
      301 cgcgaaggcc gtcggcctcc tcgcgcgtga aggcgcgaat gcgcgacgcg tccccgcccg
      361 cggccgccag catgcgcagc tgcagcgtgg cgctctcctc gagatagcac gcggcgaccg
      421 tcgcggcgcg cacgtcggga aagacggtgg tgatgccgtg gccgcgcatc atcgcgctgt
      481 tgcccttccc caccacttgc gcgagaaggt cgcccagctc gcgcgtgcgg atgaggccga
      541 cgcgcgaata ctcgggaacg ccgccggcga agatgccgcc ctggttgtga accacgcgat
      601 ggggctcgcc gatctgcgtg aggcagacgc acgagggcgg atgcgcatgc acgacgccgc
      661 ccgcctcggc ccgtgccgcg tagatgcgcg cgtggatcca gaactcgctg ttgggctcgc
      721 cctcgccgga gacgagccgc ccctcggtat cgatgaccag caggtttccc gcttccgcga
      781 ggccggggct gcggcgcgta ggaatgagga aggtgttggt gccggggatg cgcgcgcttg
      841 catggccgaa agcgtgggcg aggcccatgc gttcgaggat gcggttggcg agcgcgacgt
      901 cgcgccgcag cgcgtcgatc gtctccgcgc cgtcgctgcc ctgcgctgca tgcatcagat
      961 gactccattc gctctcaatt gcgcgatgtc cgcatcgccc atgccgagga cgccacgcag
     1021 cacgtcgtcc gtatgctcgc cgagcagcgg cgccggacga tcatgcacga tcggcgtcgc
     1081 ggaaaacttc atgggattgg cgaccatctt catttcgccc gccaccggat ggggcgcggc
     1141 cacctgcatg ccgcgatggc gcacctgcgg gtcggcgaag acctgcgaca ggtcgttgat
     1201 ggggccgcac ggcacgcccg agggctcgag cgccgcgagc cattcgcgcg cgctgcggct
     1261 tcgcattcgc gcggcgatca ttccgaccag caggtcgcgg tggcgcacgc gctcgggatt
     1321 cgtcgtgaag cgcgcatcga gcggccattc gggcgcgccg atcacctcgc agaatttcgc
     1381 gaactgggtg tcgttgccga cggcgatcac caggaactcg tcgctcgcgc ggaacacgtg
     1441 atacggcacg atcgcctggt gcgcgttgcc catgcgttgc ggcacctcgt tcgagcagaa
     1501 gtacgcggag ctcaggttgg cgagcatcgc cacctgcacg tcgaggagcg ccatgtcgat
     1561 gtactggccc cggccggtgc ggtcgcggtg cagcagcgcg gcgaggatcg cgcacgtcgc
     1621 gtacatgccg gtgaagagat ccgagacggc gacgccgacc ttctgcgggc cgcctcccgg
     1681 aagatcgtcg cgctccccgg tgacgctcat caggccgccc aggccctgca ccatgaaatc
     1741 gtatcccggg cggtcgcgat agggcccgtc ctggccgaat cccgtgatcg agcagtaaac
     1801 aagtccgggg ttgtcgcttg cgagcgacgc atagtcgagg ccgtaacggg cgagatcgcc
     1861 caccttgaaa ttctccacca gcacgtcgga tttcgccgcg agcgagcgag ccaccgattg
     1921 gcccgcgggg ctggcgaggt cgagcgtgag cgatttcttg ccgcggttgc acgcgaggaa
     1981 atacgcggcg tcgcgcgtgt cgcgcccttc gcggtccttc aggaagggcg gtccccactg
     2041 ccgcgtgtcg tcgcccaggc cggggcgctc gaccttgatc acctcggcgc cgaggtcggc
     2101 gaggttctgc gtggaccagg gacccgcgag cacgcgggtg aggtcgagga cgcgcacggc
     2161 gtcgagaatt cggctcatgg agtgtggttt cctggacggc gcggtaaacc ggatggcggt
     2221 accgtccgtt tacattgcac gcggattata gtgggccgca tcccgggaga ctgcatatgc
     2281 atccgttggg cagggccgct tgggctgcgg cgatcgggtg ctgggccgcg gcgagccacg
     2341 ccgcggagcc gaccgcgaca gcggtc
//
LOCUS       DASVII010000465         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_165110,
            whole genome shotgun sequence.
ACCESSION   DASVII010000465 DASVII010000000
VERSION     DASVII010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165110"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VF309_06075"
     CDS             complement(<1..233)
                     /locus_tag="VF309_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7466189.1"
                     /translation="MPKALLFLLLACLAPAAVFAQQSVYLEELTWTEVRDATKAGKTT
                     IIVPVGGTEQNGPHMTLGKHNARVKLLAGRIATA"
     gene            327..578
                     /locus_tag="VF309_06080"
     CDS             327..578
                     /locus_tag="VF309_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466190.1"
                     /translation="MERSTLSAAQLYAVLDREFKQLRPAACKTCHVPVPYWRTPPDDV
                     SANWHIGQPVDCPHGCHLVIAELLARLWTRYDIEQQLSQ"
     gene            634..>1641
                     /locus_tag="VF309_06085"
     CDS             634..>1641
                     /locus_tag="VF309_06085"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7466191.1"
                     /translation="MAETLVRTGPGTPMGNLMRRYWVPILLASEIAEPDGPQQRVTIM
                     GEKLLAFRDTSGHVGLIDEFCAHRGASLYFGRNEENGIRCSYHGLKYARDGTCVDVPS
                     APQVCKLMGIKAYPCIERAGIVWAYMGPKDRQPAPPEVEWCGLPDSHVFVSKRLQESN
                     YLQAMEGGIDTAHVSYVHRYEMESDPTFKGVKANDYIRADGNVVFDIERNDFGLTLYG
                     RRNGEPDSYYWRITQWLFPWFTLIPPFGHHALGGHVWVPIDDEHCWAWSMNWRPDEPL
                     NAEEREAMERGKGIHVEYIPGTFVPVANKSNDYMIDRAAQKEKRAYSGVFGFSMQDAS
                     LQ"
BASE COUNT          296 a          544 c          534 g          267 t
ORIGIN      
        1 gccgtggcga tgcgccccgc gaggagcttc acgcgcgcgt tgtgcttgcc gagcgtcatg
       61 tgcgggccgt tctgctcggt gccgcccacc ggaacgatga tcgtggtctt gcccgccttg
      121 gtcgcgtcgc gcacctcggt ccaggtgagc tcctcgaggt agacggattg ctgggcgaaa
      181 accgccgcgg gcgcgaggca ggcgagcagc aggaagagaa gggccttcgg catgggttgg
      241 catgatagcg cgcggcggca aggggcctca ttgaacgctt tgcgcgattg cacctccaac
      301 tgcggactcg acgctcgaag aatctcttgg aaagatccac cctctccgcg gcgcagctct
      361 acgccgtcct cgaccgcgag ttcaagcaac tgcgcccggc cgcctgcaag acctgccacg
      421 tgcccgttcc ctattggcgc acgcccccgg acgacgtctc ggcgaactgg cacatcggcc
      481 agccggtcga ttgcccgcat ggctgccacc tcgtgatcgc cgagctcctg gcacgcctct
      541 ggacccgcta cgacatcgag cagcagctgt cccaataagc tgcgcgataa tggggcctgg
      601 ctgactactc cacgggcaga catggaacgg gaaatggcgg aaacgctggt gcgtacgggg
      661 cccggcacgc ccatgggcaa cctgatgcgc cgctactggg tgccgatcct cctcgcgagc
      721 gagatcgccg agccggacgg cccgcagcag cgcgtgacga tcatgggcga gaagctcctc
      781 gccttccgcg acacctcggg gcacgtcggc ctcatcgacg agttctgcgc gcatcggggc
      841 gcctcgctct acttcggccg caacgaggaa aacggcatcc gctgctcgta ccacggcctc
      901 aagtacgcgc gtgacgggac ttgcgtggac gtgccctccg cgccgcaggt gtgcaagctg
      961 atgggcatca aggcgtatcc gtgcatcgag cgcgccggca tcgtgtgggc gtacatgggt
     1021 ccgaaggaca ggcagcccgc gcctcccgag gtcgaatggt gcgggctgcc cgacagccac
     1081 gtcttcgtct ccaagcgcct gcaggagagc aactacctgc aggcgatgga aggcggtatc
     1141 gacaccgcgc acgtttccta cgtgcaccgc tacgagatgg aaagcgatcc gaccttcaag
     1201 ggcgtgaagg ccaacgacta cattcgcgcc gatggcaacg tggtcttcga catcgagagg
     1261 aacgacttcg gactcacgct ttacggccgg cgcaacggcg agcccgattc gtactattgg
     1321 cgcatcacgc agtggctctt cccgtggttc acgctgatcc cgccctttgg ccatcacgcg
     1381 ctgggcggtc acgtgtgggt gccgatcgac gacgagcact gctgggcgtg gagcatgaac
     1441 tggcgccccg acgagccgct caacgccgaa gagcgcgagg cgatggaaag gggcaagggc
     1501 atccacgtcg aatacattcc cgggaccttc gttccggtcg ccaacaagtc caatgactac
     1561 atgatcgacc gcgccgcgca gaaggagaag cgcgcgtaca gcggcgtctt cggcttctcg
     1621 atgcaggacg catcgctcca g
//
LOCUS       DASVII010000466         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_345338,
            whole genome shotgun sequence.
ACCESSION   DASVII010000466 DASVII010000000
VERSION     DASVII010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345338"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..598
                     /locus_tag="VF309_06090"
                     /pseudo
     CDS             347..598
                     /locus_tag="VF309_06090"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
     gene            614..913
                     /locus_tag="VF309_06095"
     CDS             614..913
                     /locus_tag="VF309_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466192.1"
                     /translation="MTSLLSEHGDALATSLYAGVLVMTSLLSAAMWWYARSDPELMSP
                     DVPEATRREGLITTLLMGAVFMLSILIAQLWSATAAQWSWLLLIPAGHFARKMRG"
     gene            963..>1589
                     /gene="ruvB"
                     /locus_tag="VF309_06100"
     CDS             963..>1589
                     /gene="ruvB"
                     /locus_tag="VF309_06100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7466193.1"
                     /translation="MTEPERLVTALKRDTDATEVSLRPRSLSEFIGQEQARSNLKVFI
                     QAARDRGEPLDHVLFAGPPGLGKTTLAQILAKQLGVGFRSTSGPVIAKAGDLAALLTN
                     LEDRDVLFIDEIHRLNPAIEEILYPALEDYQLDLIIGEGPAARSVRIDLARFTLVGAT
                     TRLGLLTTPLRDRFGIPIRLNFYSVAELELVVERGARVLGAAISADGAH"
BASE COUNT          276 a          522 c          496 g          295 t
ORIGIN      
        1 gggctgatcg cacacgacgc agacctctgc cgcgcagagc ggccgcgcaa gcactagaac
       61 aataaaggtg agtgcgagaa ttcgcagcat gatcccgatc ttgtcacgaa ccccgcggga
      121 ggaagctatc gccatcgtac cgatcttgga agcgcaaata gaagtgtgca gcgcgatgct
      181 ttcgcatagt ggcgcggccg gcccgctttg cagcgcacaa ttgctcctgt cctgcacctt
      241 gacgcaagcc gcgaaccaga ccaccagctc gccttatctc aagcggcggg ggcacgtgaa
      301 tacgatggga tcgggcgcgg aaggccatac cttcaagccc gagcgcatgc tcgccttcag
      361 cgatgcggtc atggcggtcg ccattacgct ccttgtgctc gacctgaagc tcccgagcga
      421 ggtgagcgat gccgatctcg gcaacgcgct gcgcgctgca gtccccaacc tcgcagccta
      481 tggtctgagt ttcgtcgtca tcgggctatt gtagatcggc caccatcagc aattctcgca
      541 aatccgccgc gtcgatgggg ccctcatctg gctcaatctg ttttttctgc tgacgatcgg
      601 cctcatcccg tttgtgacga gcctgctcag cgagcacggg gatgcgctcg ccacttcgct
      661 ctatgccggg gtgctggtga tgacctcgct gctgtcggcg gcgatgtggt ggtacgcgcg
      721 gagcgatccc gagctcatgt cgcccgacgt gccggaggca acgcggcgcg aagggctgat
      781 caccacgctg ctgatgggtg cggtgttcat gctttccatc ctcatagcgc agttgtggag
      841 cgcgaccgcc gcgcagtgga gctggctgct actgataccg gctgggcatt tcgcgcgcaa
      901 aatgcggggg tgatgcaacc tggcgccttg acgatgccgt cgaacagcgg cacgaacaaa
      961 gcatgaccga gccagagcgc ctcgtcacgg ccttgaagcg cgacaccgac gccaccgagg
     1021 tctcgctgcg gccccgctcg ctatcggaat tcatcggcca ggagcaggcg cgctccaacc
     1081 tcaaggtctt catccaggca gcacgcgatc gcggcgagcc gctcgaccat gtgctgtttg
     1141 ccggcccacc gggtctcggc aagacgacgc tggcgcaaat tcttgccaag cagctgggtg
     1201 tcggctttcg ctccacctcc ggcccggtca tcgccaaggc tggcgatctc gcggcgctgc
     1261 tgaccaacct ggaagatcgc gatgttctgt tcatcgacga gatccaccgc ctgaacccgg
     1321 cgatcgagga gatcctctac cccgcgctcg aggactacca gctcgacctg atcatcggcg
     1381 aggggccggc ggcgcgctcg gtgcgcattg acctcgcgcg ctttacgctc gtcggcgcca
     1441 cgacgcggct ggggcttctg accacgccgc tgcgcgaccg tttcggcatc cccatccggc
     1501 tcaatttcta cagcgtggcc gagcttgagc tcgtcgttga gcgaggagcg cgcgtgcttg
     1561 gcgcggcaat cagcgccgat ggcgcgcat
//
LOCUS       DASVII010000467         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_488864,
            whole genome shotgun sequence.
ACCESSION   DASVII010000467 DASVII010000000
VERSION     DASVII010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488864"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF309_06105"
     CDS             complement(<1..262)
                     /locus_tag="VF309_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4072 domain-containing protein"
                     /protein_id="HEX7466194.1"
                     /translation="MNLVIQGEEIETPDLKELHRIARGEAIERISDDAFRVTKADPAA
                     RKEVAVLCEKAKLDFGWVAENRALADFGLLAMDMDSTLISIEC"
     gene            complement(448..3228)
                     /gene="mfd"
                     /locus_tag="VF309_06110"
                     /pseudo
     CDS             complement(448..3228)
                     /gene="mfd"
                     /locus_tag="VF309_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
BASE COUNT          515 a         1074 c         1167 g          578 t
ORIGIN      
        1 tgcactcgat cgagatgagg gtggagtcca tgtccatggc gaggaggccg aagtccgcga
       61 gcgcccggtt ctccgcgacc catccgaagt cgagcttcgc cttttcgcat aggaccgcga
      121 cctccttcct cgccgcggga tcggccttgg tgacgcggaa cgcgtcgtcg gagatgcgct
      181 cgatcgcctc gccgcgcgcg atgcgatgca gctccttcag gtcgggcgtc tcgatctctt
      241 cgccctggat cacgaggttc atggttgaga gttactccag cgaaaggctt tggatccgca
      301 gataaacgca gatgaacgcg gtaaaagcaa aagcagatcg gagtttcgtg ccacggatcc
      361 agtatctcga aaatccaatt gggtttcgcc cttactgcgt tcatctgcgt ttatctgcgg
      421 atccaaagcc gtttgcttgc agggatgcta ggcgagtttc ttgaggaact ggcggacctc
      481 gttggagcgc gacttcatgt cctcgtgctt catctcgatc ctcacgcggt cggggccggg
      541 aaggcggtag atgcggctcg actgcacgag ctgcagcacg cgcttcggat cgaccggcgg
      601 ctggggcacg aactgcacca cgatgccgct cgccgaggcg tcgatcttct gcacgcccag
      661 cggcgcgccc tggatgcgca gcttgtggca ttcgaggagc acgcgcgccg ggtcgggaag
      721 cgggccgaag cggtccacca gctcctcggt gaggcgctcc agctcttctt ccgactggca
      781 gttggcgagg cgcttgtaga gaacgaggcg ctcgtggacg tcgccgcaat acgcctcggg
      841 caggagcgcc ggcgcatgca ggttcacctc ggtggcgacg ctgaaggggc tcgacaggtc
      901 gggctccttg cccttcttca gggagcgcac cgcgtgctcg agcatctccg tgtagagcgc
      961 gaagcccacg tcgtggatgc cgcccgattg cgactcgccg agcacctcgc ccgcgccgcg
     1021 gatctcgagg tcgtgcatcg cgaggtagaa gccggcgccc aggtcctcca tcatctggat
     1081 cgcctcgaga cgcttcctgg ctgcggccga gagcgcatcc tcgggaggag tgaggagata
     1141 cgcgtacgcc tggtggtgcg agcgcccgac gcgtccgcgc agctggtgga gctgcgccag
     1201 gccgaacttg tcggcgcggt ggatgaggat ggtgttggcg ctcggcacgt cgatgccggt
     1261 ctcgatgatg gtcgagcaga ggagcacgtt ggagcgctgg tggtagaagt cgcgcatcac
     1321 gcgctcgagc tggcgctccg gcatctggcc atgggccacc gcgatgcgcg cttcggggag
     1381 caggcgctcc agctcctcgc gccggttctc gatggtctcg acctcgttgt acaaatagta
     1441 ggcctggccg ccgcgcttca gctcccgcag gaccgcctcg cgcacgatgc ccgcggagag
     1501 gcggttcacg aaggtcttga tggacaggcg ccgctgcggg gcggtcgcga tgaccgagaa
     1561 atcgcgcagg ccttccaggg acatcgccag cgtgcgcggg atcggcgtcg cggtgagcgt
     1621 gagcacgtcg acctcggtgc gcagtttctt cagctcctcc ttctggcgca cgccgaagcg
     1681 gtgctcctcg tcgatgatca cgaggccgag gttcttgaac ccgacgtcct tctggatcag
     1741 cttgtgcgtg ccgatcacga tatcgatggt gccggagcgc agcgcgtcga ccgtcttcgc
     1801 tacttccttc gggctgcgga agcgcgacag ctccgcaatg cgcacgggga acgccgagaa
     1861 gcgatcggtg aagttctgga agtgctgctc ggcgaggagc gtggtcggca cgaggatcgc
     1921 gacctgggcg ccgcccgcga cggcgatgaa tgccgcgcgc atcgcgacct ccgtcttgcc
     1981 gaatcccacg tcgccgcaca ccagccggtc catggggcga cccgaggtca tgtcttcgat
     2041 ggccgcgcga atcgcctcgg cctggtcggg cgtctcctcg aacgggaacg tcgcggcgaa
     2101 cgcctcgtag tcctgcgccg cgagcgggaa tgcgtgccct tgccgcgcga ggcgcttcgc
     2161 gtagaggtcg aggagctcgg ccgcggtgtc gcgcacctgg cgcgccgcct tgcgcttcgc
     2221 cttctcccat tggccgctgc cgaggcgatg caacggcgcg tcggccgggg aggcgcccgt
     2281 gtagcggctg atggcgtgga gctgcgagac ggggacgtag agcttgtccc cgccctcgta
     2341 ctcgaggtgc aggaactcca tgtcgccttc gccgaggtcg agcgtgacga gcccgagaaa
     2401 gcgtccgatg ccgtgctggc tgtgcaccac gggatcgccg gtcttcacct ccgcgagatc
     2461 gcggaccatg ccctcggcgc tcgatttggc gcgcgcatcg cggcgccgcg cctggcgcac
     2521 ctgccccgga tacagctccg cctcggtgac gaccgcgatc ttctcgccgg gcagcacgaa
     2581 gcccgatgcg accggcccgt aggcgagcgc ggcgggatgg ctcgatgcga gaaaacggct
     2641 ccagtcgtcg acgagggccg ggtggaatcc gtgctcggag aagaaggccg agatcgtctc
     2701 gcgccttccc gggctctcgg cgacgatgag cgtgcggccg ccgaacgttt cggtgaatcg
     2761 cgtgagcgcc ttgagcggct cgatcgagcg gcgatcgacc tcgaccaggg gtgcgtcgtt
     2821 tgcgtgaaga cgggaatccg ggcccgggtc cccgtcttca cgggagcggc ggctgacgtc
     2881 gatccgcgga atctccttga gcgcggtgaa caggtcttcg accggcatgt agagctcgcg
     2941 cggctcgagg aggggacggt cgcggtcgcc gcgcaacagg tcgaagcgcg atttgaggtc
     3001 cttccagaac gcctcggccg ccgcggacac gtcgccgtgg atcacgcacg tcaccggcgg
     3061 caggtagtcg aacagcgtcg ccgtcgcgtc gaagaagagc ggcaggtagc actcgacccc
     3121 cgcgggcgcg atgccgttcg acacgtcctt gtacacgcgg ctgcgcgtgg gatcgccttc
     3181 gaagcgctcg cggaaggatt cgcggaagtg cgcgcgcccc tcctcatcct ccccgcctcc
     3241 gcgcaggatg cggagcgcct gcgcgaagag atggcgtggt tcgatccgaa gctcgcggtg
     3301 catcggctgc ccgactggga aatacttccg tacg
//
LOCUS       DASVII010000468         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_165200,
            whole genome shotgun sequence.
ACCESSION   DASVII010000468 DASVII010000000
VERSION     DASVII010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165200"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VF309_06115"
     CDS             <1..894
                     /locus_tag="VF309_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7466195.1"
                     /translation="CHPDVRVWEVRDDAGKHVAYFYFDPYARRDKRSGAWMNEYRSQE
                     RFDGEVTTIVSNNSNFVKGKPGEPVLVSWHDAVTLFHEFGHGLHGLSSNVTYPSLAGT
                     NVDRDYVEFPSQLMEHWLATPEVLGKYARHCVTGEPIPAELVRRIERASKFNKGFENV
                     EYLASALVDMRMHLDPASAQDVASFERTTLEALGMPRQMAMRHRLPHFGHLFNSDSYA
                     AGYYSYLWADALSADAFEAFTEAGGAYDKSVARKLREHVFSAGNTIDPEEGYRAFRGR
                     DAQIDALMRRRGFGEGGSSRP"
     gene            891..1283
                     /locus_tag="VF309_06120"
     CDS             891..1283
                     /locus_tag="VF309_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7466196.1"
                     /translation="MSAKTVERARANRSPEEGALEALRAGYRERDAAKVLSVYAEDVE
                     ATIVNRNNPPSRPLVLRGRAELRKMVEDICSREMTHGITGTIAADGSLAYRVECRYPD
                     GCNVTGLYMATLKDGLIVREFSIDCWDE"
     gene            complement(1286..>1509)
                     /locus_tag="VF309_06125"
     CDS             complement(1286..>1509)
                     /locus_tag="VF309_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7466197.1"
                     /translation="PASLEALGITPREAEVLCWVAQGKTNREIGMILGSSARTVQKHL
                     EHVFQKIGVESRTAAILKAWQAGRHEARA"
BASE COUNT          241 a          525 c          515 g          228 t
ORIGIN      
        1 tgccatcccg acgtgcgcgt gtgggaggtg cgggacgacg ccggcaagca cgtcgcgtat
       61 ttctacttcg atccctacgc gcgccgcgac aagcgctcgg gcgcgtggat gaacgagtat
      121 cgcagccagg agcgcttcga cggcgaggtc accaccatcg tctcgaacaa ctcgaacttc
      181 gtgaagggca agcccggcga gccggtgctg gtgagctggc acgacgcggt gacgctcttc
      241 cacgagttcg gccacgggct gcacgggctt tcgtcgaacg tcacctatcc gtcgctcgcc
      301 ggaacgaacg tcgaccggga ttacgtggag ttcccgtcgc agctgatgga gcattggctc
      361 gcgacaccgg aagtgctcgg gaagtacgcg cgccactgcg tgaccggcga gcccattccg
      421 gcggagctgg tgcgcaggat cgagcgggca tccaagttca acaagggctt cgagaacgtg
      481 gagtacctgg cgagcgcgct cgtggacatg cgcatgcacc tcgacccggc gagcgcccag
      541 gacgtcgcct cgttcgagcg cacgacgctc gaggcgctcg gcatgccgcg gcagatggcg
      601 atgcgccatc gcctgccgca cttcggccac ctcttcaaca gcgactccta cgccgccggc
      661 tactacagct acctgtgggc cgacgcgctc tccgcggacg ccttcgaggc gttcaccgag
      721 gcgggcggcg cgtacgacaa gtcggtggcg cgcaagcttc gcgagcacgt cttcagcgcg
      781 ggcaacacga tcgacccgga ggagggctac cgcgcctttc gcgggcgcga tgcgcagatc
      841 gacgcgctca tgcgccgccg cggcttcggc gagggcggct cgtcgcggcc atgagcgcca
      901 agaccgtcga gcgcgcgcgc gccaaccgct ctcccgagga aggggccctc gaagcgctgc
      961 gcgcgggcta ccgcgagcgc gacgcggcca aggtgctgag cgtctacgcc gaggatgtcg
     1021 aggccaccat cgtcaaccgc aacaacccgc cgtcgcgccc gctggttctg cgcgggcgcg
     1081 cggagctgcg caagatggtg gaggacatct gctcgcgcga gatgacccat ggcatcaccg
     1141 gcaccatcgc cgccgacggc tccctcgcct accgcgtgga gtgccgctat cccgacggct
     1201 gcaacgtgac cggcctctac atggcgacgt tgaaagacgg cctcatcgtg cgggaattca
     1261 gcatcgactg ctgggacgag tagggctagg cgcgcgcctc gtggcgcccc gcctgccacg
     1321 ccttgaggat cgccgccgtg cggctttcca ccccgatctt ctggaacacg tgctcgaggt
     1381 gcttctgcac cgtgcgcgcg ctcgagccca ggatcatgcc gatctcgcgg ttggtcttgc
     1441 cctgcgcgac ccagcagagg acctcggcct cgcgcggcgt gatgccgagg gcctcgaggc
     1501 tcgccggcg
//
LOCUS       DASVII010000469         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_93164, whole
            genome shotgun sequence.
ACCESSION   DASVII010000469 DASVII010000000
VERSION     DASVII010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93164"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /gene="nuoF"
                     /locus_tag="VF309_06130"
     CDS             complement(<1..1327)
                     /gene="nuoF"
                     /locus_tag="VF309_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7466198.1"
                     /translation="MPAPAQRRIIFQHIDEPGYTNDLACYQKHGGYEVMKRAFARPPA
                     EIVDEVKKSGLRGRGGAGFPCGVKWGLLDKKSGKPIYLVVNADESEPGTFKDRYIMHQ
                     DPHQLIEGSMITCFANNVKQAYIYIRGEFPHGARILEKAIAEARAANFVGPNILGSNY
                     SCEIYVHRGAGAYICGEETGLIESLEGKRPYPRIKPPYFPAVLGLYQCPTIVNNVETL
                     CHVKHIADMGGEAFSKIGTPNNTGTRILCVSGHVQKPGYYEFEVGKITMGQLLNEVCG
                     GPLPGRKFKAVIPGGSSAKILKFGERFKGKRKVGAEMVDYDWAVEDVPMDFDSLGMIG
                     TMGGSGGVIVMDDSVNMVEALANINAFYSHESCGQCTPCREGSLWMKKITTRMVHGAA
                     RAEDAALLKSVADQIPGRTICAFGEACSWPTQSFIAKFGDEFKAYPETKK"
     gene            complement(1377..>1824)
                     /locus_tag="VF309_06135"
     CDS             complement(1377..>1824)
                     /locus_tag="VF309_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX7466199.1"
                     /translation="ITHYPVKRSALLPLLHLIQEEAGWISTEAIEWVAQKLELQPINV
                     YEVVTFYPMFRQKPIGRRHIKVCRTLSCALLGGYKTCASFEKEFNTHLGEVSPDGEVT
                     VEFVECLASCGTAPVVLIDDELHEKVDAAKAKQLSDQIKSEAKARK"
BASE COUNT          291 a          572 c          584 g          377 t
ORIGIN      
        1 ctttcttggt ttccgggtag gctttgaact cgtcgccgaa cttcgcgatg aagctctgcg
       61 tgggccatga gcacgcctcg ccaaatgcgc aaatcgtccg accgggaatc tggtcggcga
      121 cgctcttgag cagcgcggcg tcctcggcgc gggcggcgcc atgcaccatg cgcgtggtga
      181 ttttcttcat ccacagcgag ccctcgcggc agggcgtgca ctggccgcag ctctcgtgcg
      241 agtagaacgc gttgatgttg gccagcgcct ccaccatgtt gacggagtcg tccatcacga
      301 tgacgccgcc ggagccgccc atcgtgccga tcatgccgag cgaatcgaaa tccatcggca
      361 cgtcctcgac ggcccagtcg tagtcgacca tttccgcacc gaccttgcgc ttgcccttga
      421 agcgctcgcc gaacttcaaa atcttggcgg acgagccgcc gggaatgacc gccttgaact
      481 tccggccggg caacgggccc ccgcacacct cgttgagcag ctggcccatc gtgatcttgc
      541 cgacctcgaa ttcgtagtaa cccggcttct gcacgtggcc gctgacgcag aggatgcggg
      601 tgccggtgtt gttgggcgtg ccgatcttcg aaaacgcctc gccgcccatg tccgcgatgt
      661 gcttcacgtg gcaaagcgtc tcgacgttgt tcacgatcgt cgggcactgg tagaggccga
      721 ggaccgcggg aaaatagggc ggcttgatgc gcgggtacgg gcgcttgccc tcgagcgact
      781 cgatcaggcc cgtctcctcg ccgcagatgt aggcgccggc gccgcggtgg acgtagattt
      841 cgcaactgta gttcgacccg agaatgttcg ggccgacgaa gttcgccgcg cgcgcctcgg
      901 cgatggcctt ttccaggatc cgcgcgccat gcggaaactc gccgcggatg tagatgtagg
      961 cctgcttcac gttgttcgcg aagcacgtga tcatgctgcc ctcgatcagc tggtgcggat
     1021 cctggtgcat gatgtagcgg tccttgaacg tgccgggctc ggactcgtcg gcattgacca
     1081 cgaggtagat cggcttgccg ctcttcttgt cgagcagtcc ccacttcacg ccgcacggga
     1141 agccggcgcc accgcgtccg cgcaggccgg acttctttac ttcatccacg atctccgccg
     1201 gcgggcgggc aaacgcgcgc ttcatcacct cgtaaccgcc atgcttttgg tagcacgcga
     1261 ggtcgttggt atagcccggc tcgtcaatgt gctggaagat gatgcggcgt tgggcgggag
     1321 cgggcatgag aggattttcg attttggaat gtcgattttc gatttccgat gcgcgcttac
     1381 ttgcgcgcct tcgcctcact cttgatctgg tcgctcagtt gtttcgcctt ggcggcgtcc
     1441 accttctcgt ggagctcgtc gtcgatgagc acgacgggcg cggtgccaca gctcgcgagg
     1501 cactcgacga actcgaccgt gacctcgcca tcgggcgaga cctcgccgag gtgcgtgttg
     1561 aattcctttt cgaacgaggc gcacgtcttg tagccgccca ggagcgcgca ggagagcgtg
     1621 cggcagacct tgatgtggcg gcggccgatg ggcttctgcc ggaacatcgg gtagaaggtc
     1681 acgacctcgt agacgttgat gggctgcagt tccagtttct gcgcgaccca ctcgatggcc
     1741 tcggtcgaga tccagcccgc ctcctcctgg atcaggtgca ggagcggcag cagggcgctg
     1801 cgcttcaccg ggtaatgggt gatc
//
LOCUS       DASVII010000470         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_93169, whole
            genome shotgun sequence.
ACCESSION   DASVII010000470 DASVII010000000
VERSION     DASVII010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93169"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..961)
                     /locus_tag="VF309_06140"
     CDS             complement(398..961)
                     /locus_tag="VF309_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466200.1"
                     /translation="MDLLRSRAVPALIAALCVPALAGAADCSMLQGTYRFESAGPGES
                     RPLSDLTRGPGANKLYRIDAKGSAPGGLNPTQTMRRPKTTLLASTAALIHSAGRTRFR
                     FMDPAGATLVEMGIDDPVHWTCKTSRLERSAERTAGLGNVIRTERVDEVLERNQAGDL
                     VYLETVTVVDPPGAKPVRREARFPRVP"
     gene            complement(925..>2117)
                     /locus_tag="VF309_06145"
     CDS             complement(925..>2117)
                     /locus_tag="VF309_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7466201.1"
                     /translation="DRKVAIKVAKTDIEMGDEEAKRFERLFLNEASLAGKLNHPNIVG
                     VFDAVVEGDLRYIVMEYVPGGSLKKFCTETNLLPVRQAVLVVFKMCRAMDYAFQNGVI
                     HRDIKPANILLSDRDDIKISDFGTAKISHAEHTQIDGFLGSPAYMSPEQINEEGPSVQ
                     SDIYSLGVVMYELLTGKLPFRAENAVTMINKILNEDPVPIQSLRPDLPEALVDIVKKA
                     MHKDPKVRYGSWFEMASELANTFPQLERYSHEISSTEKFNKLRSLPFFREFRDAELWE
                     VLRGAVWETHSRDENLLLEGEIGQAFFIIVGGQVKVVKDGKLLNVLKEGDCFGEMAYL
                     SGDRARRSASIISVSEVQLLKIQATHLESLSEGCQLRFNREFLRTLIERLTWTSSVLA
                     QFRR"
BASE COUNT          363 a          660 c          696 g          398 t
ORIGIN      
        1 ccccaacact tcaatgaggc tctcacgagt tgggccgtgc ctgcgcagtc catcgtccca
       61 gatttatata tgtaccgctc tgatgctccc cgcatccccg ccgatagatc actggcagcc
      121 cgcttgtgcg acctgcagat actaaccgcg tcgcgcatcg ccgttcttct tggcgattca
      181 catctaaaat cctggggacg gtcggaatgt gcgcgcagag ccatccatcg aactcttggg
      241 atgaagctcg agagggggtg ccccgctcat cgcacaggag aagaactcca agaagttcga
      301 gcgctatcgt aagtagcgag ctccctcccc ctcgaaaggt cgaggtcccc cgggtcgagc
      361 ttcatcccga gttcgatgga tggcgtgaaa gggacggcta gggaactcgc gggaaacgcg
      421 cttcgcgcct gacgggcttg gcccccggcg gatcgacgac ggtcaccgtc tccaggtaga
      481 cgagatcgcc cgcctggttg cgctcgagga cttcgtcgac cctttcggtg cggatcacat
      541 tgcccaggcc cgcggtccgc tcggcgctgc gctcgaggcg gctcgtcttg caggtccagt
      601 ggaccggatc atcgatgccc atttccacga gtgtcgcgcc ggccgggtcc atgaagcgaa
      661 accgcgtacg gccggccgaa tgaatcaagg cggcggtcga cgcgaggagg gtcgttttcg
      721 gccgccgcat cgtctgcgtg ggattgagcc cgcccggagc gctgcccttg gcgtcgatgc
      781 gatagagctt gttggcaccg gggccgcgag tgagatcgga aagcgggcgg gattctcccg
      841 gcccggccga ttcgaagcga tacgttcctt ggagcatcga gcagtcggcc gcgcccgcga
      901 gcgcgggcac gcaaagcgcc gcgatcagcg ccggaactgc gcgagaacgg aggaggtcca
      961 cgtgagccgc tcgatcaacg tgcgcaggaa ctcgcggttg aagcgaagct ggcagccctc
     1021 ggagagcgac tcgaggtgcg tcgcctggat cttgaggagc tgcacctcgg agactgagat
     1081 gatcgaagcc gagcggcgcg cgcgatcgcc cgagaggtag gccatctcgc cgaagcagtc
     1141 gccttccttg aggacgttca ggagcttgcc gtccttcacc actttcacct gcccgccgac
     1201 gatgatgaag aaggcctggc cgatctcgcc ttcgaggagc aggttctcgt cgcgcgaatg
     1261 ggtctcccac acggcgcccc tcagcacttc ccagagctcg gcgtcgcgga actcgcggaa
     1321 gaagggcagc gagcgcagct tgttgaattt ctcggtggag ctgatctcgt gcgagtagcg
     1381 ctcgagctgc gggaacgtgt tggcgagctc gctcgccatc tcgaaccacg agccgtagcg
     1441 caccttcgga tccttgtgca tcgccttctt gacgatgtcc accagcgctt cgggcaggtc
     1501 cgggcgcagc gactggatcg gcaccgggtc ctcgttgagg atcttgttga tcatcgtcac
     1561 cgcgttctcg gcgcggaacg ggagcttgcc ggtgaggagc tcgtacatca cgacacccag
     1621 cgagtagatg tccgactgca cgctcgggcc ttcctcgttg atctgctcgg gcgacatgta
     1681 cgccggcgag ccgaggaagc cgtcgatctg cgtgtgctcg gcgtgcgaga tcttcgcggt
     1741 gccgaagtcg ctgatcttga tgtcgtcgcg gtcggaaagg aggatgttgg cgggcttgat
     1801 gtcgcggtgg atcacgccgt tctggaacgc gtagtccatg gcgcggcaca tcttgaagac
     1861 caccagcacc gcctggcgca ccggcaggag gttggtctcg gtgcagaact tcttgagcga
     1921 gccgcccggc acgtactcca tgacgatgta gcgcaggtcg ccttcgacga ccgcgtcgaa
     1981 cacgccgacg atgttcggat ggtttagctt gccggcgagc gaggcctcgt tcaggaacag
     2041 gcgctcgaag cgcttggcct cctcgtcgcc catctcgatg tcggtcttgg cgaccttgat
     2101 cgcgaccttg cggtcgt
//
LOCUS       DASVII010000471         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_201089,
            whole genome shotgun sequence.
ACCESSION   DASVII010000471 DASVII010000000
VERSION     DASVII010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201089"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VF309_06150"
     CDS             complement(<1..198)
                     /locus_tag="VF309_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466202.1"
                     /translation="MIFGVVLAAQAASAAPQAIRIGKDAAPLAGSLTRDTIEARTREF
                     LAKLPELKIDVRDLVLAHAEFQ"
     gene            complement(265..>1806)
                     /locus_tag="VF309_06155"
     CDS             complement(265..>1806)
                     /locus_tag="VF309_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466203.1"
                     /translation="LNPTFDGWLTAQRRHFRGCRVAFLERIVASVPDEEAFAYLDQWR
                     ELAPFDPRVHHALLAALAGQGRILEGDEHVAATTRLFEAEGLESAPLRDAWRAARSQG
                     RVEVVASASEISTTAARRRASIAVMPFAAPSAELALPGGIAHGLAHDVITRLAKLRSL
                     FVIAQGTVFALHERGIGAEEAGRRLNVDYVVSGSVRSRGGRFNATIELVETRSARIVW
                     TEAYDHKLDDAFLFLDEIGDRIVASIAAEIETLESARAILKPPSSLDAWEAYHRGLWH
                     MYRYRKADNAEAKRIFLAALKLDPTFSRTYAGLAFTHYQDAFQGWGEREQAMDRAYEV
                     ASQGLMIDDRDPAVHWAMGRALYLRQRWDESVAELERSIDLSPNFALGHYNLSFVRSV
                     VGDPNVAITDAELSQRLSPHDPMLFGMLATRAMSLVRLERFEDAAGWAVKAAARPNAF
                     PHIHAIAAYSLALAGSMDEARLHAAAARRTAPGYGISEFLLTFPFEPRGEELFRRGAK
                     LVGME"
BASE COUNT          285 a          585 c          651 g          285 t
ORIGIN      
        1 ctggaattcc gcgtgggcga gcacgaggtc gcgaacgtcg atcttcagct cgggaagctt
       61 cgcgaggaat tcccgcgtgc gggcttcgat cgtgtcgcgg gtcagcgacc ccgcgagggg
      121 cgccgcatcc ttgccgatgc gaatcgcctg cggcgcggcg cttgccgcct gggcggcgag
      181 cacgacaccg aagatcaatg caacgaaggc gtgcgcgagc gatgagcggg ctgcgaacag
      241 tttttttgtg cctcccagga agtgctactc catgccgacg agttttgcgc ctctgcgaaa
      301 cagctcttcg cctcgcggct cgaatggaaa ggtgagaagg aattccgaga tgccgtaacc
      361 gggcgccgtg cgccgcgcgg ccgccgcgtg caagcgagcc tcgtccatcg agccggccag
      421 cgcgagcgag tacgccgcga tggcgtggat gtgcgggaag gcgttgggcc gcgcggcggc
      481 cttgacggcc catccggcgg cgtcctcgaa ccgctcgagg cgcacgaggg acatggcgcg
      541 cgtcgcgagc attccgaaga gcatcggatc gtgcgggctc aaccgctgcg agagctcggc
      601 gtcggtgatg gcgacgttcg gatcgcccac gacggagcgg acgaacgaca ggttgtagtg
      661 cccgagcgcg aaattcgggc tgaggtcgat cgatcgctcc agctcggcca cgctctcgtc
      721 ccatcgctgg cgcaggtaca gggcgcgccc catggcccaa tgcaccgcgg ggtcgcgatc
      781 gtcgatcatg agtccctgcg atgcgacttc gtacgcgcga tccatggcct gctcgcgctc
      841 gccccaaccc tggaacgcat cctggtagtg cgtgaaggca aggccggcgt acgtgcgcga
      901 aaaggtcggg tcgagcttca gcgcggcgag gaagatccgc tttgcctcgg cgttgtccgc
      961 cttgcgatag cggtacatgt gccacaggcc gcggtggtac gcctcccacg cgtcgagcga
     1021 gctgggcggc ttgaggatcg cgcgggcgct ctcgagcgtc tcgatttccg ccgcgatcga
     1081 ggcgacgatg cgatcgccga tctcgtcgag gaacaggaag gcgtcgtcga gcttgtggtc
     1141 gtacgcctcc gtccagacga tccgcgcgct gcgcgtctcc acgagctcga tggtcgcatt
     1201 gaaacgcccg cctcgagagc gcaccgatcc gctgacgaca tagtcgacgt tgagcctccg
     1261 gccggcctct tcggctccga tgccgcgctc gtgcagcgcg aacaccgtgc cctgcgcgat
     1321 gacgaacagg ctccgcagct tggcgagccg cgtgatgacg tcgtgggcaa ggccgtgcgc
     1381 gatgccgccc gggagcgcca gctcggcgga aggggcggcg aacggcatca ccgcgatcga
     1441 ggcgcggcgg cgcgccgccg tggtggatat ttcggaggcg ctcgcgacga cctcgactct
     1501 tccttgcgag cgcgccgcgc gccaggcgtc gcgaagcggc gcgctttcga gcccttcggc
     1561 ttcgaagagg cgcgtggtcg cggcaacgtg ctcatcgccc tcgaggatgc gaccttgtcc
     1621 tgcgagcgcc gcgaggaggg cgtgatgaac gcgtgggtcg aacggcgcaa gctcgcgcca
     1681 ctggtcgaga taggcgaatg cctcttcgtc gggaacgctc gcgacgatgc gctcgaggaa
     1741 cgccacccgg cagccgcgga agtggcgccg ctgcgcggtg agccagccgt cgaacgtggg
     1801 gttgag
//
LOCUS       DASVII010000472         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_237716,
            whole genome shotgun sequence.
ACCESSION   DASVII010000472 DASVII010000000
VERSION     DASVII010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237716"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VF309_06160"
     CDS             <1..1256
                     /locus_tag="VF309_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7466204.1"
                     /translation="PREIDAVRAHCGARRALFLGDESPDARHHAERVQATRLSLGRWG
                     TISVSAMDERCMPEPVEGKPARDVAALLYTTGTTGEPKGVMLTHANLLFIARVSAELR
                     GITPDDRVFGLLPISHVYGLASVCLGTLRAGASLHLQARFIPAAMAECLSSKAITVCQ
                     GVPAMYAKLLDHLKARGTRLAAPALRFLYAGGSPLPLALKREVEATLGLTLHNGYGLT
                     EASPTITQTRHDEPRADCSVGRALPGVEVRIVDREANDLAPGEIGELWVRGPNVMKGY
                     YRDPEGTRAALKAGGWLATGDLARQGEDGAYFIEGRLKELIIRSGFNVFPVEVEGVLN
                     SHPEVTQSAVVGRPADGDEEVVAFVELVPSAQVTPAELLEWAAASLAPYKRPAEIVVM
                     ASLPHAPNGKVLKGRLAQMARERHA"
     gene            complement(1261..1782)
                     /locus_tag="VF309_06165"
     CDS             complement(1261..1782)
                     /locus_tag="VF309_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7466205.1"
                     /translation="MKYAENLFARRDPPARPMRDPAAPPAQATTHPSVHPTTSAPAAA
                     AVRPAAPAPSLDPITTRTEEPGGSKLIVGPNIKLKGVEIDDCDTLVVEGRVEATMVSR
                     AIQIAENGAFIGKVDVDVAEIRGDFKGEMTARKKLVIHASGRVSGKIRYGKMLVQEGG
                     EMSGDIGALAANE"
BASE COUNT          260 a          662 c          659 g          303 t
ORIGIN      
        1 cgccacgcga gatcgacgcc gtgcgcgcgc attgcggcgc gcgccgcgcg ctcttcctgg
       61 gcgacgagtc gcccgacgcc cgccaccacg ccgagcgcgt gcaggccacg cgcctgtccc
      121 tggggcgctg gggcaccatc agcgtgagcg ccatggacga gcgctgcatg cccgagccgg
      181 tcgagggcaa gcccgcgcgc gacgtggccg cccttctcta tacgaccggg accacgggcg
      241 agccgaaggg cgtgatgctc acccatgcca acctgctctt catcgcgcgc gtctccgcgg
      301 agctgcgcgg catcacgccc gacgatcgcg tcttcggcct cctgcccatc tcgcacgtgt
      361 acgggctcgc ctccgtgtgc ctgggcacct tgcgcgctgg cgcgagcctg cacctgcagg
      421 cgcgcttcat cccggccgcg atggccgagt gcctttcgtc caaggcgatc accgtgtgcc
      481 agggcgtccc cgcgatgtac gcgaagctcc tcgatcacct gaaggcgcgc ggcacgcgcc
      541 tcgccgcgcc ggcgctgcgc ttcctgtatg ccggcggatc gccgctgccg ctcgcgttga
      601 agcgcgaggt cgaggccacg ctcggcctca cgctccataa cggctacggg ctcacggaag
      661 cgtccccgac catcacgcag acgcgccacg acgagccgcg cgccgattgc tcggtgggcc
      721 gggcgctgcc gggcgtggag gtgcggatcg tcgaccgcga ggccaacgac cttgcgcccg
      781 gcgagatcgg cgagctctgg gtgcgcgggc cgaacgtgat gaagggctac taccgcgacc
      841 cggaaggcac gcgcgcggcg ctcaaggcgg gcggttggct cgccacgggg gatctcgcac
      901 gccagggcga ggacggcgcc tatttcatcg aggggcggtt gaaggagctc atcatccgct
      961 ccggcttcaa cgtctttccc gtcgaggtcg agggcgtgct caactcccac cccgaggtca
     1021 cgcaatcggc ggtggtcgga cggccagccg acggcgacga ggaggtggtc gcattcgtcg
     1081 agctcgtgcc gagcgcgcag gtcacgccgg cggagcttct cgagtgggcc gccgcgtcgc
     1141 tcgcgcctta caagcgcccg gcggagatcg tcgtgatggc gagccttccg cacgcgccca
     1201 acggcaaggt cctcaagggc cgcctcgcgc agatggcgcg agagcggcac gcctgaaggg
     1261 ttattcgttc gcagcgagcg cgccgatgtc gccgctcatc tcgccgcctt cctgcaccag
     1321 catcttgccg tagcggatct ttcccgacac gcgcccgctc gcgtggatca cgagcttctt
     1381 cctcgccgtc atctcgccct tgaagtcgcc gcggatctcg gccacgtcga cgtcgacctt
     1441 gccgatgaaa gcgccgttct cggcgatctg gatggcgcgg ctcaccatgg tcgcctcgac
     1501 gcgcccctcg accaccagcg tgtcgcaatc gtcgatctcc acgcccttca gcttgatgtt
     1561 cggtccgacg atgagcttgg agccgccggg ctcctcggtg cgcgtagtga tcggatcgag
     1621 ggagggggcc ggagcggcgg gccgcacggc ggcggccgcg ggcgcggaag tagtggggtg
     1681 aacggacgga tgggtggtgg cctgcgcggg cggggctgcg ggatcgcgca tcggccgcgc
     1741 cggaggatcg cggcgcgcga agagattctc tgcgtatttc atcgttggac cttttccctt
     1801 ggttggaaca tcggggaggt cacactcacg attcgtgcca agcgcgatga aatcggccgt
     1861 ttctcctctg gggaagcctg tgtt
//
LOCUS       DASVII010000473         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_345538,
            whole genome shotgun sequence.
ACCESSION   DASVII010000473 DASVII010000000
VERSION     DASVII010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345538"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF309_06170"
     CDS             complement(<1..478)
                     /locus_tag="VF309_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX7466206.1"
                     /translation="MSAYPLLETVDDPAELRALDRKQLPQLATELRAFIVDSVASTGG
                     HLSSNLGTVELTIALHYVFNTPEDKLIWDVGHQTYGHKILTGRRKGMGTLRQKGGIAG
                     FPRREESRYDTFGTAHSSTSISAAIGMAVAARRQGSDRRVVAVIGDGAMTAGMAFEA"
     gene            complement(494..1357)
                     /locus_tag="VF309_06175"
     CDS             complement(494..1357)
                     /locus_tag="VF309_06175"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX7466207.1"
                     /translation="METALAEFLPSPQTVPMRLHDAMRYATLGGGKRVRAMLVFGAGE
                     ATGAQPRRLEIVAAAVEMIHAYSLIHDDLPCMDDDVLRRGKPTCHVEFDEATALLAGD
                     ALQSLAFQLLADHRLAQGGDAQLEMIKLFAAACGSRGMAGGQAIDLDSVGRGITLPEL
                     EFMHILKTGALIRASVLMGAQCGSESGGAAIAAAELERLDRYAKCVGLAFQVVDDILD
                     EEGDSSVLGKTAGKDRAAGKPTYTSLMGLAEAKRFALELLEDSREALAGFGERAERLR
                     EIADFIVHRRR"
     gene            complement(1402..1644)
                     /locus_tag="VF309_06180"
     CDS             complement(1402..1644)
                     /locus_tag="VF309_06180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7466208.1"
                     /translation="MSAKAQGNSPSELSFEKALEELEALVARMEDGKLPLEESLAAYQ
                     RGAELVKFCESKLVDAQARIAILEGDTLRDFTPESK"
BASE COUNT          301 a          607 c          625 g          319 t
ORIGIN      
        1 gcgcctcgaa cgccatgccc gccgtcatcg cgccatcgcc gatgaccgcc accacgcgac
       61 ggtccgagcc ctgccggcgc gccgccaccg ccatgccgat cgccgccgag atcgacgtcg
      121 acgaatgcgc ggtgccgaag gtgtcgtagc ggctttcctc gcgccgcggg aagcccgcga
      181 tgccgccctt ctggcgcagc gtccccatgc ccttgcggcg tcccgtgaga atcttgtggc
      241 cgtaggtctg gtggcccacg tcccagatga gcttgtcctc gggcgtgttg aacacgtagt
      301 gcagcgcgat ggtgagctcg accgtgccca ggttggagga caggtggccg ccggtcgatg
      361 cgaccgaatc gacgatgaac gcgcgcagct ccgtggcgag ttgcggaagc tgcttgcggt
      421 cgagcgcccg caactctgcg ggatcgtcga ccgtttcgag aagggggtag gcactcatgg
      481 aaagagtccc attctaacgc cggcggtgga cgatgaaatc cgcgatctcc cggagccgct
      541 cggccctctc gccgaagccc gcgagcgcct cgcgcgaatc ctcgagcagc tcgagggcga
      601 agcgcttcgc ttcggcgagt cccatgaggc tcgtgtaggt gggcttgccg gcggccctgt
      661 ccttgcccgc ggtcttgccg aggaccgacg aatcgccttc ctcgtcgagg atgtcgtcga
      721 ccacctggaa ggcgaggccc acgcacttcg cgtagcgatc gaggcgctcg agctccgcgg
      781 ccgcgatcgc ggcgcccccc gattccgacc cgcattgcgc gcccatcagc accgaggcgc
      841 ggatgagggc gccggtcttg aggatgtgca tgaactcgag ctcgggaagc gtgatgccgc
      901 ggccaaccga atcgaggtcg atcgcctggc cccccgccat gccgcgcgag ccgcacgcgg
      961 cggcgaagag cttgatcatc tcgagctgcg catcgccgcc ctgcgcgagg cgatggtcgg
     1021 cgaggagctg gaacgccagg ctctgcagcg catcgcccgc gaggagcgcg gtggcctcgt
     1081 cgaactcgac atggcacgtg ggcttgccgc gccgaagcac gtcgtcgtcc atgcatggaa
     1141 ggtcgtcgtg gatgagcgag tacgcgtgga tcatctcgac cgcggcggca acgatctcga
     1201 ggcggcgcgg ttgcgcgccg gttgcttctc cggcgccgaa cacgagcatc gcgcgcacgc
     1261 gcttgccgcc gccgagggtc gcgtagcgca tcgcgtcgtg gagccgcatg gggaccgttt
     1321 gcggcgacgg gaggaattcc gcgagcgccg tttccatccg ggcagccacg tcacgcgacc
     1381 acgcctggaa atccggcgcc ttcacttcga ttcgggggtg aagtcgcgca acgtgtcgcc
     1441 ttcgaggatc gcgatgcgcg cctgcgcatc caccagcttc gactcgcaga acttcacgag
     1501 ctccgcgccg cgctggtagg cggcgaggga ttcctcgagc ggaagcttgc cgtcctccat
     1561 gcgggcgacg agcgcctcga gctcctcgag ggccttctcg aagctcaatt cggacgggga
     1621 attgccttgc gctttcgcgg acatcgactg tcgccttgcg aacatatgcc aggggatgaa
     1681 ttttacacca aaggtcttga aaaataaggg aactcgcctc aatataggag tgggacggag
     1741 ctgcagagtt aaaagctagc tgactcgatt tgcccgcatg ttgttttcat cttcgtttgt
     1801 actgggggtg cggataatgt cggatctcgc gagtcagaga ctgctcacgc gc
//
LOCUS       DASVII010000474         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_705118,
            whole genome shotgun sequence.
ACCESSION   DASVII010000474 DASVII010000000
VERSION     DASVII010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705118"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VF309_06185"
     CDS             complement(<1..1230)
                     /locus_tag="VF309_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX7466209.1"
                     /translation="MNETKKRLLLVDAPSYFYRAFHAIRELRSPAGEPTNAIYGVVNM
                     LRKLKQDFPSDYIACVLDPRGKTFRDDIYPQYKATRQAMPEDLARQIDPLLETIRALG
                     WPVVCVDGVEADDVIGTLVRHAESHGWDSVISTGDKDLTQLVNASVTWVNTMAVPIEK
                     LDREGVRAKFGVPPELIIDYLALMGDAVDNVPGVEKVGPKTACKLIEEYGSLEGVMAH
                     ADEVKGVVGENLRKAKDWLPTGRILLTVKTDVALPFEMESLVAQGPDTEKLRELYTRF
                     GFRTMRDALDNKAAPDSKDTLFNKEDAPGKGEPSASPGRAPASKPGRNFALLTAVEQP
                     VENVTPFVATRKYETIFDEAALDRWVAILEAAPIACVDTETTSLDPMVAELVGISFSA
                     SPGEAAYLPLTHRYGGVP"
     gene            1251..>1661
                     /locus_tag="VF309_06190"
     CDS             1251..>1661
                     /locus_tag="VF309_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7466210.1"
                     /translation="MAITAPKVPKPSDPQLIKRSQSSAREAWRVFEIIAEFVTATERL
                     QTIQPAVSIFGSARTAPGHRYYGVAEDIAKRLSEAGFSVISGGGPGIMEAANKGAFEG
                     ASPSVGLNIQLPHEQSGNKYQDISHTFQHFFARKV"
BASE COUNT          265 a          552 c          545 g          299 t
ORIGIN      
        1 cggcacgcct ccatagcggt gcgtcaacgg gaggtacgcg gcttctccgg gcgacgccga
       61 gaacgaaatg ccgacgagct ccgccaccat cggatcgagg ctcgtggtct cggtgtcgac
      121 gcaggcgatc ggcgccgcct cgaggatcgc cacccagcgg tcgagcgcgg cctcgtcgaa
      181 gatggtctcg tacttccgcg tcgcgacgaa gggcgtcacg ttctcgacgg gctgctcgac
      241 ggcggtgagg agcgcgaagt tgcggccggg cttcgaggcc ggcgctctcc cgggcgaagc
      301 ggagggctcg cccttcccgg gcgcgtcctc cttgttgaag agagtgtcct tgctgtcggg
      361 cgcggccttg ttgtccagcg cgtcgcgcat cgtgcggaat ccaaagcgcg tgtagagctc
      421 gcgcagcttc tccgtgtcgg ggccctgggc gacgagcgat tccatctcga agggcagcgc
      481 gacgtcggtc ttcaccgtga gaaggatccg ccccgtggga agccagtcct tcgccttgcg
      541 caggttctcg cccacgacgc ccttcacttc gtcggcgtgc gccatcacgc cctcgagcga
      601 gccgtattcc tcgatcagct tgcacgccgt cttcggcccc accttctcga cgccgggaac
      661 gttgtcgacc gcgtcgccca tgagcgcgag gtagtcgatg atgagctcgg ggggcacgcc
      721 gaacttcgcc ctcacgccct cgcggtcgag cttctcgatg gggacggcca tcgtgttcac
      781 ccaggtcacg ctcgcgttca cgagctgcgt caggtccttg tcgcccgtgg agatcaccga
      841 gtcccatccg tggctctcgg cgtggcgcac cagggtcccg atgacgtcgt cggcctccac
      901 gccgtcgacg caaaccactg gccagccgag ggcgcggatc gtctcgagga gcggatcgat
      961 ctggcgcgcg aggtcctcgg gcatcgcctg ccgcgtcgcc ttgtattgcg gatagatgtc
     1021 gtcgcggaag gtcttccccc ggggatcgag gacgcaggcg atataatccg aagggaaatc
     1081 ctgcttgagc ttgcgcagca tgttgaccac gccgtagatg gcgttggtcg gctctcccgc
     1141 cgggcttcgc agctcgcgaa tcgcgtggaa cgcgcggtag aagtagctgg gcgcgtcgac
     1201 gagcagcagg cgtttcttgg tttcgttcat ctcaatcggc aggttccgcc atggccatca
     1261 ccgcccccaa ggttccaaaa ccgtcggatc cccagctcat caagcgttcg cagagctcgg
     1321 cgcgcgaggc gtggcgggtg ttcgaaatta tcgcagagtt cgtcaccgcg accgaaaggc
     1381 tgcagacgat acagcccgcg gtttccatct tcggcagcgc ccgcaccgcg cccgggcatc
     1441 gctactacgg tgtggccgag gacatcgcca agcgcctgtc ggaagcgggc ttctcggtga
     1501 tttccggagg cggcccgggg atcatggaag cggcgaacaa gggcgcgttc gagggcgcct
     1561 ccccgagcgt gggcctgaac atccagcttc cccacgagca atccggaaac aaatatcagg
     1621 acatctcgca cacatttcaa cacttcttcg cgcgcaaggt c
//
LOCUS       DASVII010000475         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_345622,
            whole genome shotgun sequence.
ACCESSION   DASVII010000475 DASVII010000000
VERSION     DASVII010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345622"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1203)
                     /locus_tag="VF309_06195"
     CDS             complement(127..1203)
                     /locus_tag="VF309_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7466211.1"
                     /translation="MTPKERVMTALRRGQPDQVPWVENEVEELLQIQIMGGRTDYTPG
                     ELCRALGMDGFGYHFPTGAAGSASQALQTSKSVKEAFYFPKKITFDFVPPWIADMGVE
                     SSGRTFVKKGLLTDRDSLKLFAEFLPDPHHPARYEQIAKWIEQYREDYAVFARIRLGT
                     ASLIESMGLDVFSLMLYDDPDLVKEMHLRFSEWTIKVLEHLNKLDLDFYWANDDHADT
                     KMPWVNMEMYEEFFYPYQKMVADAIKKPWAFHSDGNIFPILPGLLKLGMNAIHPIQPA
                     AMDIGKMKAQYGSKVCLIGNIDLDYTLTLGTPEEVDKEVKERIAVAGKGGGYIISSAN
                     SLTDYCKIDNVWAMSKALKKYGRY"
     gene            complement(1227..>1712)
                     /locus_tag="VF309_06200"
     CDS             complement(1227..>1712)
                     /locus_tag="VF309_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX7466212.1"
                     /translation="EKYSSGEFFLPQMMLGARAMSEALQMLKPYLAQGTYERKGKAVV
                     GTVAGDFHDIGKNIVKIMLEGGGYEVVDLGVDVSPEAFVAAVKQEAPRFILMSALITL
                     TMDSMRRTIQALEAAGVRKQVLIGIGGAPVTQKFADEIGADFYGSDAHGAVEKCNQLL
                     H"
BASE COUNT          271 a          541 c          532 g          368 t
ORIGIN      
        1 tgcgcaccgg ccgcagcctg ggcgcgcacc tcatgcgcga tcgccgcggc atcgagtttt
       61 tccagcgcgg cgcggatcgc cttgcgcgag gcgttgatgc gttctgcaac gatcagcata
      121 tgcgcctcag tacctgccgt atttcttcag cgccttcgac atcgcccaga cattgtcgat
      181 cttgcagtaa tcggtgaggc tgttggcgct gctgatgatg tagcccccgc ccttgcccgc
      241 gactgcgatg cgctccttca cctccttgtc cacctcttcg ggggtgccca gggttagggt
      301 gtaatcgagg tcgatgttgc cgatcaggca caccttcgat ccgtactgcg ctttcatctt
      361 gccgatgtcc atggccgcgg gctggatcgg gtggatcgcg ttcatgccga gcttgagcag
      421 ccccgggagt atcggaaaga tattgccgtc gctgtggaag gcccagggct tcttgatcgc
      481 gtcggcgacc atcttctggt acgggtagaa gaactcctcg tacatttcca tgttgaccca
      541 gggcatcttg gtgtcggcat ggtcgtcgtt ggcccagtag aaatcgagat ccagcttgtt
      601 cagatgctcg agcaccttga tggtccattc ggaaaagcgc aggtgcattt ccttcaccag
      661 gtccgggtcg tcgtagagca tgagcgagaa tacatcgagg cccatgctct cgatgaggct
      721 ggcggtgccg aggcggatgc gcgcgaacac ggcgtagtcc tcgcggtatt gctcgatcca
      781 tttggcgatc tgctcgtagc gcgccggatg atgcggatcg ggcaggaact cggcgaacag
      841 cttgagcgaa tcgcggtcgg tgagcaggcc tttcttgacg aaggtgcgcc cgctcgactc
      901 cacgcccatg tcggcgatcc acggcggcac gaagtcgaag gtgatcttct tcgggaaata
      961 gaaggcctct ttcaccgact tcgaggtctg cagcgcctgg ctggccgagc cggcggcgcc
     1021 ggtgggaaag tgatagccga agccgtccat gcccagggcg cggcacagtt cgccgggagt
     1081 gtagtcggtg cgcccgccca tgatctggat ctgcagcagt tcctcgactt cgttctcgac
     1141 ccagggcacc tggtcgggct ggccgcggcg cagggctgtc atcacgcgtt ctttgggtgt
     1201 catctcgcct gtctccctcg cgccgctcaa tgcagcaact ggttgcattt ctcgacggcg
     1261 ccgtgcgcgt ccgaaccgta gaagtcggcg ccgatttcgt cggcgaattt ctgcgtcacc
     1321 ggcgctccgc cgatgccgat cagcacctgc ttgcgcaccc ccgcggcctc cagcgcctgg
     1381 atggtcctgc gcatgctgtc catggtgagg gtgatgagcg cgctcatcag gatgaagcgc
     1441 ggcgcctctt gtttcaccgc ggcgacgaat gcctcggggc tcacgtccac gcccaggtcc
     1501 acgacctcgt agccgccgcc ctcgagcatg atcttgacga tgttcttgcc gatgtcgtgg
     1561 aagtcgcccg cgaccgtgcc gaccaccgcc ttgcccttcc tctcgtaggt accctgcgcc
     1621 aggtagggct tcaacatctg cagcgcctcg ctcatcgcgc gcgcgccgag catcatctgc
     1681 ggcaggaaga attcgcccga ggaatatttc tc
//
LOCUS       DASVII010000476         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_58110, whole
            genome shotgun sequence.
ACCESSION   DASVII010000476 DASVII010000000
VERSION     DASVII010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58110"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF309_06205"
     CDS             complement(<1..401)
                     /locus_tag="VF309_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7466213.1"
                     /translation="MSENLPAPEEPKVERKSTWAPLRLPTFRVLWLVWMASNVCMWMN
                     DVASAWLMTSLTSSPTLIALVQTASSLPVFLFGLPSGALADILDRRRYFLVSQFWIAS
                     TAALLCSFALAGHLTAPVLHALVFTNGIGLA"
     gene            476..1408
                     /locus_tag="VF309_06210"
     CDS             476..1408
                     /locus_tag="VF309_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7466214.1"
                     /translation="MPRSAPAFHASPYLLLTLTILFWSINWVIGRAITGHVSPLAFTF
                     IRWVGALAVMLPFAWPQVRDQWPAIRKRWRLVAWLGFWGTGLHNMFAYAGLQYTTATN
                     GVILNSSVPIMIIVIGWLVYRDTITRVQALGVAVSLVGVLAILTHGDPAILAQLRLNK
                     GDLIVLAGMIFWAAYTVFLRMKPPEISGLALLACCATVGVVLLLPLFVVEMLFFGGHV
                     ELTFATAAAMAYVAVFPSFVGYVFWNRAVHEIGSNVAGIFMHLMPVFGSLLAWIFLDE
                     RIQPYHVAGIGLILGGITLTTRGRRVVPVAEPEP"
     gene            1455..>1573
                     /locus_tag="VF309_06215"
     CDS             1455..>1573
                     /locus_tag="VF309_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7466215.1"
                     /translation="MELILWRHAEAEDAGGGGDLARSLTKRGRKQAAKMAEWL"
BASE COUNT          247 a          513 c          538 g          275 t
ORIGIN      
        1 atcgcgagcc cgatgccgtt ggtgaagacg agggcgtgga gcaccggcgc ggtgagatgc
       61 ccggcgagcg cgaagctgca cagcagggcc gccgtcgacg cgatccagaa ctgggaaacc
      121 aggaagtagc ggcgccggtc gaggatgtcg gcgagcgcgc cgctcggcag gccgaacagg
      181 aacaccggga gcgacgacgc ggtctgcacc agcgcgatca gcgtgggcga ggaggtgagc
      241 gacgtcatga gccaggccga cgccacgtcg ttcatccaca tgcagacgtt ggacgccatc
      301 cagacgagcc agagcacccg gaacgtgggc aggcgcagcg gcgcccaagt cgatttcctc
      361 tcgactttcg gctcttcggg cgcggggaga ttctcgctca tggatgcggg ctggtcatcg
      421 gtggaaaggt tatcacctgc gtataatcgc ggctcccgca cgcagcccat cttcgatgcc
      481 ccgcagcgcc ccggcgttcc acgcgtcgcc gtatctcctg ctcaccctca cgattctctt
      541 ctggtcgatc aactgggtga tcggccgggc gatcacgggg cacgtgtcgc cgctcgcgtt
      601 caccttcatc cgctgggtgg gagcgctcgc ggtgatgctg cccttcgcgt ggccccaggt
      661 ccgcgaccaa tggcccgcga tccgcaagcg ctggaggctc gtcgcgtggc tcgggttctg
      721 gggcacggga ctccacaaca tgttcgccta tgcggggctg caatacacga ccgccaccaa
      781 cggcgtgatc ctcaacagct ccgtgcccat catgatcatc gtcatcggat ggctcgtgta
      841 tcgcgacacg atcacgcgcg tgcaggcgct cggcgtggcg gtctcgctcg tgggcgtgct
      901 cgcgatcctc acgcacggcg atcccgcgat cctcgcgcag ctgcgtctca acaagggaga
      961 cctcatcgtc ctcgcgggga tgatcttctg ggcggcgtat accgtgttcc tgcgcatgaa
     1021 accaccggag atctcgggcc tcgcgctcct tgcgtgctgc gcgacggtgg gcgtcgtgct
     1081 cttgctgccg ctcttcgtcg tcgagatgtt gttcttcggc gggcacgtcg agctcacctt
     1141 cgcgaccgcc gccgcgatgg cgtacgtggc ggtgtttccg tcgttcgtgg gctacgtctt
     1201 ctggaatcgc gcggtgcacg agatcggatc caacgtcgcc ggaatcttca tgcacctgat
     1261 gccggtgttc ggaagcctcc tcgcgtggat cttcctcgac gagcgcatcc agccgtatca
     1321 cgtcgccggc atcgggctga tcctgggcgg catcaccctc accacgcgcg ggcgccgcgt
     1381 cgtgccggtc gcggagcccg agccgtgatc gtttggcgcg cggcgcggtc gaacacggaa
     1441 tagcgaggcg agcgatggag ctgattctct ggaggcacgc ggaagccgag gatgccggcg
     1501 gcgggggcga ccttgcgcgc agcctcacga agcgagggcg caagcaggcg gcgaagatgg
     1561 ccgaatggct gaa
//
LOCUS       DASVII010000477         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_633058,
            whole genome shotgun sequence.
ACCESSION   DASVII010000477 DASVII010000000
VERSION     DASVII010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633058"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VF309_06220"
     CDS             <1..827
                     /locus_tag="VF309_06220"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466216.1"
                     /translation="LAAPAPVEPDFDAAEAFIWQPEREGFEPVGQVNRVSLELLKGLE
                     AVAPQLAENTERFASHLPANNALLWGARGMGKSSLVKAVHADVRGRLAKELQAQGTDL
                     KLVEIHREDVGSLPRCLEALRKSDHRFIVFCDDLSFDKDDTSYKSLKAVLEGGIEGRP
                     GNVIFYATSNRRHLMPRDMVENERSTAISPSEAVEEKVSLSDRFGLWLGFHNCSQETF
                     LDMVRAYAAHYRLKMKDEELAREALEWSMTRGARSGRVAWQFIQEAAGRLGKRLEP"
     gene            complement(1125..1773)
                     /locus_tag="VF309_06225"
                     /pseudo
     CDS             complement(1125..1773)
                     /locus_tag="VF309_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          288 a          583 c          566 g          336 t
ORIGIN      
        1 gcctggcggc tcctgccccg gtcgagcccg atttcgacgc ggccgaggcg ttcatctggc
       61 agcccgaacg cgaaggcttc gagcccgtcg gtcaggtaaa ccgcgtttcg ctcgagctct
      121 tgaagggcct cgaggccgtg gccccgcagc ttgccgaaaa taccgagcgc tttgcctccc
      181 acctgcccgc caacaacgcg ctgctttggg gcgcgcgcgg catgggcaaa tcttccctgg
      241 tcaaggcggt gcatgccgac gtgcgcggcc ggctcgccaa ggagctgcag gcgcagggca
      301 cggacctgaa gctggtcgag atccatcgcg aggacgtcgg ttcgctgccg cgctgtctcg
      361 aagcgctgcg caagtccgac caccgcttca tcgtgttctg cgacgacctc tccttcgaca
      421 aggacgatac gagctacaaa tccttgaagg ccgtgctcga gggtgggatc gagggccggc
      481 ccggcaacgt gatcttctac gcaacttcca accgccgcca tctcatgccg cgcgacatgg
      541 tggagaacga gcgctcaacc gccatctctc cctcggaggc ggtggaggag aaggtctcgc
      601 tgtcggatcg cttcggccta tggctcggct ttcacaactg cagccaggaa accttcctcg
      661 acatggtgcg cgcgtacgct gcccactacc ggctgaagat gaaggacgag gagctcgccc
      721 gcgaagcgct cgaatggagc atgacgcgcg gagcgcgctc gggccgcgtc gcctggcagt
      781 tcattcagga agcggcggga cgcctcggca agcgcttgga gccctgaggc gcaggcggaa
      841 gccgttgcag gcgccgacgc cgagccgccg tgagccgctc ggcatcgaat gactggttct
      901 cagcctcagt cggtcttgcg gcgcggcacc gaggggacgg cgcccgtccc tttgaccggc
      961 cgatcctttt tgtccttcgg gcctgggagt gccttgcgcg gggcggtctc gacgagaacc
     1021 ggttgcggct cctcgtcttc ctcctcctcc ttggatttgg cgcgcttgct cggatcctgc
     1081 gcaaagtact cgaagctgag cttgcggtcg tcgccgttgc ccacaacagt caccttcaca
     1141 gtgccgccgt gctcgagctt tccgaacagc agctcatccg cgagcggttt cttgatgtgc
     1201 tcctggatca cgcgtccgag cgggcgggcg ccaaacttct cgtcgtagcc cttctccgcc
     1261 agccacttcg tagcttcctc ggtcaactcg atcatcacgc cgcgatcggc gagctgggct
     1321 tcgagctgga agatgaactt ctccacgacc agcaggatca cctcgggcgg cagaccggcg
     1381 aacgtcacca ccgcatcgag acggttgcgg aactccggcg taaacatccg gttgatcgcc
     1441 tcgttgtcct cgccgtcgcg cttggagcga ttgaagccca ttgccggctt ggccatgtcg
     1501 ctggcaccgg cgttggtcgt catgatgagg atgacgttgc ggaagtcgac cgacttgccg
     1561 gtgtggtcgg tcagcttgcc gtggtccatc acctgcagaa ggatgttgaa cagatccgga
     1621 tgcgccttct cgatctcgtc gagcaggagc acgcaatggg gatgctggtc gatgccgtcg
     1681 gtcagcaggc cgccctggtc gaaaccgacg tagccgggcg gcgcaccgat caagcggctc
     1741 accgtgtgct tctccatgta ttccgacatg tcg
//
LOCUS       DASVII010000478         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_22622, whole
            genome shotgun sequence.
ACCESSION   DASVII010000478 DASVII010000000
VERSION     DASVII010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22622"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF309_06230"
     CDS             <1..307
                     /locus_tag="VF309_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX7466217.1"
                     /translation="RNWLSRVAAVVSYPVIKRFRRRVDHRRYNGAALLGLKGIVVKSH
                     GSADRLAFATALARAASEASHGLIERIGEEIGRIHALTPGALTPGTPTPAAPSERAA"
     gene            304..1263
                     /locus_tag="VF309_06235"
     CDS             304..1263
                     /locus_tag="VF309_06235"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7466218.1"
                     /translation="MTYSRITGTGSYLPEKLVTNRDLESLVETSDDWIVARTGIRERH
                     IAAEGEFTSDLALHACRSALAAAGRGPGDVDLIVVATSTPDMVFPSSACLLQAKLGVK
                     RGAAFDLQAVCSGFVYALGTADLFIRSGQSRCALVVGAEIFSRILDWKDRGTCVLFGD
                     GAGAVVLEASDTPGILSSHLHADGSYASILNTPGHVSAGAVQGDPTLKMDGGAVFKLA
                     VRVLEESAREALAANAMKIEDLDIYIPHQANVRIVSHVAKKLGIPEAKCIVTVDRHAN
                     TSAASIPLALDVAVRDGRVRKGHLVLLQGVGGGFTWGSVLLRY"
     gene            1267..>2018
                     /gene="fabD"
                     /locus_tag="VF309_06240"
     CDS             1267..>2018
                     /gene="fabD"
                     /locus_tag="VF309_06240"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7466219.1"
                     /translation="MSLAIVFPGQGSQSVGMMQGFSDTPIVEKTFLEANAILGVDLWT
                     MVRDGPAEALNQTVNTQPVMLIAGVACWRAWQERSGSMPSWLAGHSLGEYSALVAANS
                     IRFEDALALVRFRAQAMQEAVPEGTGGIAAILGLADGALAEVCVEASQGEVVEPANLN
                     SPGQVVVAGHRGAVERAMAIAKRMGAKRAVMLPMSAPSHCSLMKPAAERLRERLEALE
                     VRKPMIHVIHNRFVESFDDPARIRKALVEQLY"
BASE COUNT          308 a          709 c          694 g          307 t
ORIGIN      
        1 gcgcaactgg ctctcgcgcg tggcggccgt cgtctcctac ccggtgatca agcgctttcg
       61 ccggcgcgtc gaccaccggc gctacaacgg cgcggcgctt ctcggcctca agggcatcgt
      121 cgtgaagagc cacggctcgg cggaccgcct cgctttcgcc accgcgctcg cgcgcgccgc
      181 ctccgaagcg agccacggcc tcatcgagcg catcggcgag gaaatcgggc ggattcacgc
      241 cctgaccccg ggcgcgctga caccgggtac tccgacgccg gccgcgccgt cggaaagggc
      301 cgcatgacgt actcccgcat caccggcacc gggagctacc tgcccgaaaa gctcgtcacc
      361 aaccgcgatc tggaatcgct cgtcgagacg agcgacgact ggatcgtcgc gcgcaccggg
      421 atccgcgaac gccacatcgc cgccgagggc gagttcacga gcgatctcgc gctccacgca
      481 tgccgcagcg cgctggccgc cgcggggcgc gggcccgggg acgtcgacct catcgtggtc
      541 gcgacctcga cgcccgacat ggtgtttccc tcgagcgcgt gcctgctgca ggcgaagctc
      601 ggcgtgaaga ggggcgcggc gttcgacctg caggcggtgt gctcgggctt cgtctacgcg
      661 ctcggcaccg ccgatctctt catacgctcc ggccagagcc gctgcgcgct ggtggtggga
      721 gccgagatct tctcgcgcat cctcgattgg aaggatcgcg ggacgtgcgt gctcttcggc
      781 gacggcgcgg gcgccgtggt gctcgaagcc tccgacacgc ccggcatcct ctcgagccac
      841 ctgcacgcgg acggcagcta tgcgtccatc ctgaacacgc cgggccacgt ctccgccggc
      901 gcggtgcagg gcgatccgac gctcaagatg gatggcggcg ccgtcttcaa gctcgcggtg
      961 cgcgttctcg aggagagcgc gcgcgaggcg ctcgccgcca acgcaatgaa gatcgaggac
     1021 ctcgacatct acattcccca ccaggccaac gtgcgcatcg tctcgcacgt ggcgaagaag
     1081 ctcggcatcc ccgaagcgaa gtgcatcgtc acggtcgatc ggcacgccaa cacgtcggcg
     1141 gcgtcgattc ccctcgcgct cgacgtcgcg gtccgcgacg ggcgcgtgcg caaggggcac
     1201 ctcgtgctgc tgcagggcgt gggcgggggc ttcacctggg gctccgtgct cctgaggtat
     1261 tgacccatgt cgctcgcgat cgtcttcccc ggacagggct cccaatcggt cggcatgatg
     1321 cagggcttct ccgacacgcc catcgtcgag aagacgtttc tcgaggcgaa tgcgattctc
     1381 ggcgtggacc tctggacgat ggtgcgcgac ggccccgcgg aggccctcaa ccagaccgtc
     1441 aacacgcagc cggtgatgct gatcgcgggc gtggcctgct ggcgcgcgtg gcaggagcgg
     1501 tcggggtcga tgccgtcgtg gctcgcgggc cacagcctcg gcgaatacag cgcgctcgtg
     1561 gccgcgaact cgattcgctt cgaggacgcg ctcgcgctgg tgcgcttccg cgcccaggcc
     1621 atgcaggagg cggtgcccga aggcaccggc ggaatcgccg cgatcctcgg gctcgcggac
     1681 ggcgcgctgg ccgaagtgtg cgttgaggcg tcgcaaggcg aagtcgtcga acccgccaat
     1741 ctcaattcgc cggggcaggt cgtggtcgcg ggacatcgcg gcgcggtcga gcgcgccatg
     1801 gcgatcgcca agcgcatggg cgccaagcgc gcggtgatgc tgccgatgag cgcgccctcc
     1861 cattgcagcc tcatgaagcc cgcggccgag cgcctgcgcg agcgcctcga ggcgctcgag
     1921 gtgaggaagc cgatgatcca cgtcatccac aaccgcttcg tcgagtcttt cgacgatccc
     1981 gcgaggattc ggaaagcctt ggtagaacag ctctacaa
//
LOCUS       DASVII010000479         4885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_489525,
            whole genome shotgun sequence.
ACCESSION   DASVII010000479 DASVII010000000
VERSION     DASVII010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489525"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VF309_06245"
     CDS             <1..1289
                     /locus_tag="VF309_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX7466220.1"
                     /translation="APVTDAAAPQTREDLLKILDRETAVARATNGRLAVLLVELRRVD
                     RLGALLRGPAPAVTMSLVLERLRKALREDDRMAAISDEQACVILPRIAHPSQATLAAV
                     KILRVLDRPIAYEGGSAVLRPCVGIATLPEHGFDPAELLMAADVSRHIAATREEGYHV
                     YQGDEAIEAEVYHGLDLELERALRANELEVHYQPIIEVMTGLPAGIEALVRWRHPKAG
                     EIPPRTIVGIAERTGLIGSLTYWILNSALRQASQWRAERIAPRLAINLSVSTLTDHEL
                     PAVVDQGIRTWGMDPAHISLEIAESSMIADAERSVAILTRLKAIGVRLSIDDFGSGFS
                     SLSHFKRFPIDEIKIDCPFVQGMVGDKGDRAVVASAIDLAHHFGLTVVAEGVERDETR
                     AALRSLRCELAQGNLFSPPLTAVQLREWWLAHAPLA"
     gene            1299..2597
                     /gene="lplT"
                     /locus_tag="VF309_06250"
     CDS             1299..2597
                     /gene="lplT"
                     /locus_tag="VF309_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEX7466221.1"
                     /translation="MKRGFYTIMAAQFFSSLADNALLIAAIAMLVQLDGPPWLTPLLK
                     FFFTISYVVLAFLAGAFADSMPKGRVMFVTNMVKVVGCGLILFNEYLRIGDMHGYWLV
                     LLAYAVVGLGAAGYSPAKYGILTELLPPEMLVVANAWIEGLTVGSIVIGNVVGGMLIG
                     PKVSSVLLSFDFPFIDTSVDTAPEAAIVVIAIIYVVAALFNLAIPDTGARYPHQERNP
                     AKLLVDFSRCFVTLWRDKLGQISLAVTTLFWGAGACLQFIVIKWAERALHLPLEQGAM
                     LSGVSAIGIALGAVLAARYVPLKRATQVLPVGILMGVAVISMISLRWMPAIYVVLIAI
                     GAIAGFFVVPMNALLQHRGHVLMSAGHSIAVQNFNENVSILVMLALYSLMIRYDMRIQ
                     SVVLIFGLFISGAMYLVMRWNAKNHRLDPHLDRFIGEVKH"
     gene            complement(2730..3629)
                     /locus_tag="VF309_06255"
     CDS             complement(2730..3629)
                     /locus_tag="VF309_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7466222.1"
                     /translation="MRIFPTQARKILAHPGAFTLQTLKAFTANQGLLLAGAVAYYALL
                     SLVPLMILIAIALAHWIDPAQLLDSLGRYLEWLVPGQSKPIVDELANFLNHKDVIGWV
                     LLATMVFFSGLAFTVLENAMSVIFFHRVAERKRRYIVSALLPYCYILTLGVGALLITL
                     VSGSLQAMADRDIEFLGRTWSLGGVSGVLLYLLGFVGEVFVLTSIYLVMPVGRLKFTH
                     ALIGGVTAAVLWEITRHVLTWYFATLSQVSVVYGSLTTAIIVLLSLEFAAILLLLGAQ
                     VIAEYERIAHESITASPKPMRVD"
     gene            complement(3667..>4885)
                     /locus_tag="VF309_06260"
     CDS             complement(3667..>4885)
                     /locus_tag="VF309_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7466223.1"
                     /translation="EVHVEVRREGAPSSWFPLGAGEFFGELGLISGRRRSSTVKAGPD
                     CVLIEAPRRAMLKLIASVESIRKKVDEKFLKRAVRAYLAPMLPEAELDELLADGVQVK
                     TYGGGEELFKEGDAPDGLYLIRRGSVTISRTIAGREVVLSYLAAGNYVGEMALLSNSP
                     RMATVKAAVTTEALILNAATFQRVLARNPKWREEMETLFLERLRINAAMEARPDPGNI
                     IGFLMQQGVGEATDVLLIDESLCVRCNNCEKACADTHDGNSRLNREAGPTFAQIHVPT
                     SCRHCEHPHYMKECPPDAIHRSAEGEVFINDTCIGCGNCERNCPYGVIQLAAVDPNRQ
                     RPSLWSWLALGMGPEPGMEAKVKSKEVPKKAVKCDMCKDLEGGAACVRACPTGAAIRV
                     SPEQFLNYAGPSD"
BASE COUNT          755 a         1679 c         1637 g          814 t
ORIGIN      
        1 ccgcgccggt caccgatgcc gcggcgccgc agacccgcga ggacctgttg aagatcctcg
       61 atcgcgagac cgcggtcgcg cgcgccacca acgggcgcct cgcggtgctg ctggtcgagc
      121 tgcgccgcgt cgaccgcctg ggggccctgc tgcgcgggcc cgcgcccgcg gtcacgatgt
      181 cgctggtgct cgagcgattg cgcaaggcgc tgcgcgagga cgaccgcatg gccgcgatca
      241 gcgacgagca ggcgtgcgtc atcctgccgc gcatcgccca tccgagccag gccacgctcg
      301 cggcggtgaa gatcctgcgg gtgctcgacc gacccatcgc atacgagggc ggttcggcgg
      361 tgctgcgtcc gtgcgtgggc atcgcgaccc ttcccgagca cggcttcgat ccggcggaat
      421 tgctgatggc ggccgacgtc tcgcgccaca tcgccgcgac gcgcgaagag ggatatcacg
      481 tctaccaggg cgacgaggcc atcgaggccg aggtgtatca cgggctcgac ctcgagctgg
      541 agcgcgcgct gcgcgccaac gagctcgagg tccactacca gccgatcatc gaggtcatga
      601 cgggcctgcc cgccggcatc gaggcgctgg tgcgctggcg ccatccgaag gcgggcgaga
      661 tcccgcccag gaccatcgtc ggcatcgccg agcgcaccgg gctcatcgga tccctcacct
      721 actggatcct caattcggcg ctgcggcagg cgtcgcaatg gcgcgccgag agaatcgcgc
      781 cgcggctcgc gatcaacctg tccgtgagca cgctcaccga ccacgagctc cccgcggtgg
      841 tcgaccaggg catccgcacc tggggcatgg atcccgcgca catctcgctc gagatcgccg
      901 agagctcgat gatcgccgac gccgagcgct cggtcgcgat cctcacgcgg ctcaaggcga
      961 tcggggtgcg gctgtcgatc gacgacttcg gctccggctt ctcgtcgctc tcgcatttca
     1021 agcgcttccc gatcgacgag atcaagatcg actgcccgtt cgtgcagggc atggtcggcg
     1081 acaagggcga ccgcgcggtg gtcgcgagcg ccatcgacct cgcgcaccac ttcgggctca
     1141 ccgtcgtcgc cgagggtgtc gagcgcgacg agacgcgcgc ggcgctgcgc agcctgcgct
     1201 gcgagctcgc gcagggaaat ctcttcagcc cgcctctcac ggcggtgcag ctgcgcgagt
     1261 ggtggctggc ccatgcgccg ctcgcctgag aagccgcgat gaaacggggc ttctacacga
     1321 tcatggcggc gcagttcttc tcgtcgctcg ccgacaacgc gctgctcatc gccgcgatcg
     1381 ccatgctggt ccagctggac ggcccgccgt ggctcacgcc gctgctcaag ttcttcttca
     1441 ccatctccta cgtggtgctc gcgttcctgg ccggcgcctt cgccgactcg atgcccaagg
     1501 ggcgggtgat gttcgtcacc aacatggtga aggtggtcgg ctgcgggctg atcctcttca
     1561 acgagtacct gcgcatcggc gacatgcacg gctattggct cgtgctcctc gcctacgcgg
     1621 tggtgggcct cggggcggcg ggctattcgc ccgcgaagta cgggatcctc acggagctcc
     1681 tcccccccga gatgctggtc gtcgccaacg cctggatcga ggggctcacg gtggggtcca
     1741 tcgtgatcgg caacgtggtg ggcggaatgc tcatcggccc gaaggtttcc tcggtcctcc
     1801 tcagcttcga ctttcccttc atcgacacca gcgtcgacac ggcgccggag gcggcgatcg
     1861 tggtgatcgc gatcatctac gtggtcgccg cgctcttcaa cctggcgatt cccgacaccg
     1921 gcgcgcgcta tccgcaccag gagcgcaatc cggcgaagct cctcgtcgac ttctcgcgct
     1981 gcttcgtcac cctctggagg gacaagctcg gccagatctc gctcgcggtg accacgctct
     2041 tctggggcgc gggcgcgtgc ctgcagttca tcgtgatcaa gtgggcggag cgcgcgctgc
     2101 acctgccgct cgagcagggg gcgatgctct cgggggtctc ggcgatcggc atcgcgctgg
     2161 gggcggtgct cgcggcgcgc tacgtgcccc tgaagcgcgc cacacaggtg ctccccgtcg
     2221 gcatcctcat gggcgtcgcc gtgatcagca tgatctcgct tcgctggatg cccgcgatct
     2281 acgtcgtgct catcgcgatc ggcgcgatcg cgggattctt cgtggtgccg atgaacgcgc
     2341 tgctgcagca ccgcgggcac gtgctgatgt cggcgggcca ctcgatcgcc gtgcagaact
     2401 tcaacgagaa cgtgagcatc ctcgtgatgc tcgcgctcta ctcgctcatg atccgctacg
     2461 acatgcgcat ccagtcggtg gtgctcatct tcgggctctt catctccggg gcgatgtatc
     2521 tcgtcatgcg ctggaacgcc aagaaccatc gcctcgatcc gcacctcgac cgtttcatcg
     2581 gcgaggtgaa gcactagcgg cgctcccccg ggtcgagaag aaccgcccct tccacgcctg
     2641 gccgatcacc ggattgttga atcccacgcc tggccgatcg ccggattgtt gaatcccacg
     2701 cctggccgat caccggattg ttgaatcaac tagtccacgc gcatcggctt gggcgacgcc
     2761 gtgatcgact cgtgggcgat gcgctcgtat tcggcgatca cctgggcgcc gaggagcagc
     2821 aggatcgcgg cgaattccag gctgagcagc acgatgatcg ccgtcgtgag cgatccatag
     2881 acgacgctca cctgcgacag cgtcgcgaaa taccacgtga ggacgtggcg cgtgatctcc
     2941 cagagcaccg ccgccgtcac gccgccgatg agcgcgtggg tgaacttcag gcgccccacc
     3001 ggcatcacga ggtagatcga ggtgaggacg aagacctcgc ccacgaagcc cagcaggtag
     3061 agcagcacgc cggagacgcc gccgagggac cacgtgcggc cgaggaactc gatgtcgcga
     3121 tccgccatcg cctgcaggct cccggagact agcgtgatga ggagcgcgcc cactcccagc
     3181 gtgaggatgt agcaataggg cagcagcgcg gaaacgatgt agcggcgctt ccgctcggcc
     3241 acgcggtgga agaagatcac cgacatcgcg ttctccagca ccgtgaacgc gagcccgctg
     3301 aagaagacca tggtcgcgag gagcacccag ccgatcacgt ccttgtggtt gaggaagttc
     3361 gcgagctcgt cgacgatcgg cttcgactgt cccggcacca gccattcgag gtagcgcccg
     3421 agggagtcga ggagctgcgc ggggtcgatc cagtgcgcga gcgcgatcgc gatgaggatc
     3481 atgagcggga cgagcgaaag gagcgcgtag taggcgaccg cgcccgccag gagcaggccc
     3541 tggttcgccg tgaaggcctt cagcgtctgc agcgtgaacg cgcccggatg ggcgagtatc
     3601 ttgcgggcct gcgttgggaa gatgcgcatc gtctgcgtgg acgcgagggc gcgctcgacc
     3661 ccgcgctcag tcgctgggcc cggcgtaatt caggaactgc tccgggctca cgcggatcgc
     3721 cgcgcccgtg gggcaggcgc gcacgcacgc cgccccgccc tcgaggtcct tgcacatgtc
     3781 gcacttcacc gccttcttcg ggacctcctt cgacttcacc ttggcctcca tgccgggctc
     3841 gggccccatg ccgagggcca gccacgacca gaggctcggg cgctgccggt tcgggtcgac
     3901 cgccgcgagc tggatcaccc cgtacgggca gttgcgctcg cagttgccgc acccgatgca
     3961 ggtgtcgttg atgaacacct ccccctcggc cgagcggtgg attgcatcgg gagggcattc
     4021 cttcatgtag tgcggatgct cgcagtggcg acacgaggtc ggcacgtgga tctgcgcgaa
     4081 tgtcggcccc gcttcgcgat tgaggcgcga gttgccgtcg tgagtgtcgg cgcacgcctt
     4141 ctcgcagttg ttgcagcgca cgcacagcga ctcgtcgatg aggaggacgt ccgtcgcttc
     4201 ccccacgccc tgctgcatga ggaagccgat gatgttgccc ggatccgggc gcgcttccat
     4261 cgccgcgttg atgcgcagcc gctcgaggaa gagcgtctcc atctcctcgc gccacttcgg
     4321 gttgcgcgcg agcacgcgct ggaaggtggc cgcgttgagg atgagggcct cggtcgtcac
     4381 cgcggccttc acggtggcca tgcgcgggga attcgacagg agcgccatct cgcccacgta
     4441 gttgccggcg gcgaggtacg agagcacgac ctcgcgcccc gcgatcgtgc gcgagatggt
     4501 caccgagccc cggcggatga ggtacagccc gtccggcgcg tcgccctcct tgaatagctc
     4561 ctcgccgccg ccgtaggtct tcacctgcac gccgtcggcg aggagctcgt cgagctcggc
     4621 ttcgggaagc atcggcgcga ggtaggcgcg caccgcgcgc ttgaggaact tctcgtcgac
     4681 cttcttgcgg atcgactcga ccgacgcgat gagcttcagc atggcgcgcc gcggcgcctc
     4741 gatgaggacg cagtcgggcc ccgccttcac ggtgctcgag cgccgccgcc ccgatatgag
     4801 gccgagctcg ccgaagaatt cacccgcccc gagcgggaac cagctagagg gcgcgccctc
     4861 gcgccgcacc tcgacgtgca cttcg
//
LOCUS       DASVII010000480         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_669637,
            whole genome shotgun sequence.
ACCESSION   DASVII010000480 DASVII010000000
VERSION     DASVII010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669637"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VF309_06265"
     CDS             complement(<1..1178)
                     /locus_tag="VF309_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7466224.1"
                     /translation="MHDALPVALILLACAVAVVVLFRQLALPAILGYLIVGATIGPHA
                     LAFVPATESQRYLGEFGVVFLMFSVGLEFSLPQLNNMRRIVFGFGGLQVAAITALGVA
                     ACVAIGLSWREGIVVGGVAAMSSTAIVSRMLAERMQLHSDYGRQVMGVLLFQDLAVIP
                     LVVLIPALALTRGEIAMAIAVALGKAAVVLAIVLFVGQRVMRGLFEMVARQKSSEIFV
                     LFVLLVTLGLAWVTEVAGLSLALGAFLGGMLISETEYRYQVEDYIKPFRDVLLGLFFV
                     TIGMLLDPRTLADHFLAVAAVFAAMLVVKFVAIFALGRLFGNAKPTALRAALTLAPAG
                     EFGFVLLSLAQREEALGAMTLQVVLAAALLSMLATPLLLSNMDRLVLYFVESEWTQRA
                     V"
     gene            1205..>1989
                     /locus_tag="VF309_06270"
     CDS             1205..>1989
                     /locus_tag="VF309_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX7466225.1"
                     /translation="MTNLLQPHALADAEHFLELARGVLDIEADAVRALKGRLGEAFLR
                     AHRMILETKGRVVVTGMGKSGHVARKIASTLASTGTPAFFMHPAEASHGDLGMITSQD
                     VVIAISYSGESDEIMKVLPVIKRRGAKVIAITGRAQSTLATEADVHLDAGVEKEACPL
                     DLAPTASTTATLALGDALAMALLEARGFKSDDFAMHHPGGALGRRLLVHVSDIMRKGE
                     HLPKNAPGDMLPEVILEMSKKGIGMTCIVDAGDGLLGIYTDGDL"
BASE COUNT          379 a          666 c          691 g          253 t
ORIGIN      
        1 acggcgcgct gcgtccactc gctctcgacg aagtagagga cgaggcggtc catgttcgag
       61 agcagcagcg gcgtggcgag catcgacagc agcgccgcgg cgagcaccac ctggagcgtc
      121 atcgcgccca gcgcctcctc gcgttgcgcg agcgagagca ggacgaagcc gaactcgccc
      181 gcgggggcga gcgtgagcgc ggcgcgcagc gccgtgggct tcgcgttgcc gaagagccgc
      241 cccagcgcga agatcgccac gaacttcacg acgagcatcg cggcgaacac cgcggcgacc
      301 gcgaggaaat gatcggcgag cgtgcggggg tcgaggagca tgccgatggt cacgaagaaa
      361 agccccagca gcacgtcgcg gaacggcttg atgtagtcct cgacctggta gcggtattcg
      421 gtctccgaga tgagcatgcc gccgagaaag gcaccgaggg cgagggacag gcccgccacc
      481 tcggtcaccc acgcgaggcc gagcgtcacc agcagcacga agagcacgaa gatctccgac
      541 gacttctggc gggcgaccat ctcgaagagg ccgcgcatca cgcgctggcc cacgaagagc
      601 acgatcgcga gcaccaccgc cgccttgccg agtgccaccg cgatcgccat cgcgatctcg
      661 ccgcgggtca acgcgagcgc ggggatcagc accacgaggg gaatcacggc gaggtcctgg
      721 aagaggagca ctcccatgac ctggcggccg tagtcgctgt gcagctgcat gcgctcggcg
      781 agcatcctgg acacgatggc ggtcgaagac atggccgcca cgccgcccac gacgatcccc
      841 tcgcgccacg acagcccgat ggccacgcag gcggcgacgc cgagggcggt gatcgcggcg
      901 acctgcagcc cgccgaagcc gaagacgatg cggcgcatgt tgttcagctg cggaaggctg
      961 aactcgaggc ccacggagaa catgaggaac accacgccga actcgccgag gtagcgctgg
     1021 ctctcggttg cggggacgaa cgcgagcgcg tgcgggccga tggtggcgcc cacgatgagg
     1081 tagcccagga tcgccggtag cgcgagctgg cggaagagca ccaccaccgc cacggcgcag
     1141 gcgaggagga tcagtgccac cggcagggcg tcatgcatct tggaatctat ttatactttc
     1201 gcgtatgacg aatctactac aaccccacgc gctcgccgac gcggagcatt tcctcgagct
     1261 cgcgcgcggg gtcctcgaca tcgaggccga cgcggttcgc gcgttgaagg gccgcctcgg
     1321 cgaggccttc cttcgagccc atcggatgat cctcgagacg aaggggcgcg tggtggtcac
     1381 cggcatgggc aagtcggggc acgtggcgcg caagatcgcg tcgaccctcg catcgacggg
     1441 aacgcccgcc ttcttcatgc atccggcgga agcgagccac ggcgacctcg gcatgatcac
     1501 ctcgcaggac gtggtgatcg ccatctcgta ttcgggcgag tccgacgaga tcatgaaggt
     1561 gctgccggtg atcaagcggc gcggagcgaa ggtgatcgcg atcaccggcc gcgcgcagtc
     1621 gacgctcgcc accgaggccg acgtgcatct cgacgcgggg gtcgagaagg aagcctgccc
     1681 gctcgacctc gcccccaccg cgagcaccac ggcgacgctc gcgctgggag acgcgctcgc
     1741 catggcgctc ctggaggcgc gcggcttcaa gagcgacgac ttcgcgatgc accacccggg
     1801 aggcgccctc gggcggcgcc tcctcgtgca cgtgtccgac atcatgcgca aaggcgagca
     1861 tctcccgaag aacgcccctg gagacatgct ccccgaggtg atcctcgaga tgtcgaagaa
     1921 ggggatcggc atgacgtgca tcgtggacgc cggggacggc ctcctcggca tctacacgga
     1981 cggggacct
//
LOCUS       DASVII010000481         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_525570,
            whole genome shotgun sequence.
ACCESSION   DASVII010000481 DASVII010000000
VERSION     DASVII010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525570"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VF309_06275"
     CDS             complement(<1..433)
                     /locus_tag="VF309_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7466226.1"
                     /translation="MNDISAPALVSYADVEAAAQRLEGVAHRTPVATSSQFDKLTGAQ
                     AFFKCENLQRMGAFTFRGAYNALAQLGEEARGRGVVAFSSGNHAQAVALAGSLLGIPT
                     VIVMPADAPAVKVAATRGYGAQVVFYDRAAGEDRDQVARRIA"
     gene            complement(462..668)
                     /locus_tag="VF309_06280"
     CDS             complement(462..668)
                     /locus_tag="VF309_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX7466227.1"
                     /translation="MNPDTFNMSIRKFLKTVGVHSQREIETAVERARAAGKLQGNETL
                     DAKMTLTVDGVALNVAFDGKISLE"
     gene            complement(688..930)
                     /locus_tag="VF309_06285"
     CDS             complement(688..930)
                     /locus_tag="VF309_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466228.1"
                     /translation="MTAAVENTGTFMLSKEIEILMAERARLLRVAGAAAQFVAVMDPK
                     SLPEKVATEADILAESVNALAEDTLKDALNAITTPR"
     gene            complement(974..>1805)
                     /locus_tag="VF309_06290"
     CDS             complement(974..>1805)
                     /locus_tag="VF309_06290"
                     /inference="COORDINATES: protein motif:HMM:NF039736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX7466229.1"
                     /translation="NVSAAMLDAVEDADGRDNWREFLRFRDRVASLPSLEAAYRDLYS
                     RAEVDLAPPFVDALTQAITRSILDGTDDAFLCRAGEMLFRRQRVATEGGQTLAADSAT
                     VEAYADTGGFGSVGRLLRSQDALPTVKMDVLNGENAPVYFLRDELYSFALDLTPGREG
                     ARSLAEVLARWVRHLAGADVAIEPAERIEDGAWRWHVGLDIDATAILNALYRGEEVAA
                     EDLERIVVLFRLEFRNPRDALAEMAGRPVYLAVACRPDRTLRLKPQNLLINLPLAASQ
                     "
BASE COUNT          253 a          614 c          645 g          293 t
ORIGIN      
        1 ccgcgatgcg ccgcgccacc tggtcgcgat cttcgcccgc ggcccggtcg tagaagacga
       61 cctgcgcgcc gtagccccgc gtggcggcga ccttcacggc gggcgcgtcg gcgggcatga
      121 cgatcaccgt cggaattccc agcaggctgc ccgcgagcgc gaccgcctgc gcgtggttgc
      181 ccgaggagaa cgcgaccacg ccgcggcccc gcgcctcctc gccgagctgc gcaagcgcgt
      241 tgtacgcgcc gcggaacgtg aaggcgccca tgcgctgcag gttctcgcac ttgaagaacg
      301 cctgggcgcc ggtgagcttg tcgaactggc tcgaggtcgc gacgggcgtg cggtgggcga
      361 cgccctcgag gcgctgcgca gcggcctcga cgtcggcgta ggaaacgagc gcgggggcgg
      421 aaatgtcgtt catggggcgc gccttcccgg gcgcggcgat ctcactcgag cgagatcttg
      481 ccgtcgaagg cgacgttgag cgcgacgccg tccacggtga gcgtcatctt ggcgtcgagc
      541 gtctcgttgc cctggagctt ccccgcggcc cgggcgcgct cgacggcggt ctcgatctcg
      601 cgctgggagt gcacgccgac cgtcttgagg aacttgcgga tgctcatgtt gaaggtgtcg
      661 ggattcatcg tctcgttcct ttgggtgcta gcgcggcgtg gtgatcgcgt tgagcgcgtc
      721 cttcaacgtg tcctccgcga gcgcgttcac gctctcggcg aggatgtcgg cctcggtggc
      781 gaccttctcc ggaaggctct tcgggtccat cacggcgacg aattgcgccg cggcgcccgc
      841 cacgcgcaac aggcgcgcgc gctccgccat gaggatctcg atctccttgc tcagcatgaa
      901 ggtcccggtg ttctcgactg cggctgtcat gacttgctcc tgggttcgaa cctgcaattc
      961 tacgtgagcg gcgtcactgc gacgccgcca gcggcaggtt gatgaggagg ttctgcggct
     1021 tcaggcgcag cgtgcggtcg ggacggcacg cgaccgccag gtacacgggt cgcccggcca
     1081 tctccgcgag ggcgtcgcgc ggattcctga attcgaggcg gaagaggacg acgatccgct
     1141 cgaggtcctc ggcggcgacc tcttctcccc ggtaaagagc gttgagaatc gccgtcgcgt
     1201 cgatgtcgag ccccacgtgc caccgccacg cgccgtcctc gatgcgctcg gcgggctcga
     1261 tcgcgacatc ggcgcccgcc aggtggcgca cccatcgcgc caggacctcg gcgagagagc
     1321 gcgcgccctc gcggcccggc gtgaggtcga gggcgaagct gtagagctcg tcacgcagga
     1381 aatagaccgg cgcgttctcg ccgttgagca cgtccatctt caccgtgggg agcgcatcct
     1441 ggctgcgaag caggcgcccc acgctgccga agcctcccgt gtcggcgtac gcctcgaccg
     1501 tcgcgctgtc tgcggcaagc gtctgcccgc cctcggtcgc cacgcgctgc cggcggaaga
     1561 gcatctcgcc ggcgcggcac aggaacgcgt cgtcggtgcc atcgaggatc gagcgcgtga
     1621 tcgcctgcgt gagggcgtcg acgaagggcg gggcgaggtc cacttcggcg cgcgagtaga
     1681 gatcgcgata tgccgcctcg agggacggca ggctggcgac gcgatcgcga aagcgcagga
     1741 actcgcgcca gttgtcgcgt ccgtcggcgt cttcgacggc gtccagcatc gccgcggaaa
     1801 cgttc
//
LOCUS       DASVII010000482         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_669822,
            whole genome shotgun sequence.
ACCESSION   DASVII010000482 DASVII010000000
VERSION     DASVII010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669822"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF309_06295"
     CDS             <1..342
                     /locus_tag="VF309_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466230.1"
                     /translation="TLACIVAIAQSGVFAARVPATTRHGLDVVAIFAVACALIGIATV
                     QRRIDVYPLALIAASAIAVSTTLLVHNIKLNDLGDFFVISIWLIATSTAAGLMLMRWV
                     RAWRTDAGANA"
     gene            339..1331
                     /locus_tag="VF309_06300"
     CDS             339..1331
                     /locus_tag="VF309_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HEX7466231.1"
                     /translation="MIATAQDLVKELRARGMVTAQPPIAESPATGGDRPWYIGLLLGT
                     AGWFAGVFALIFVMLLSRPTAGYNALAVGIVLLGAAWGIFRADREGAFFSQLALALSI
                     AGQFTTIYGIHALFFKGANSIAGVAAIALVLQALLVLVMPNRLHRTMSTLFACTAWAI
                     AVRYILWDEPGRNSQLPPSLGLVLVGFAIAWLPVGAKLYALIRTEARWIASGRQALLR
                     PVTTGLIAGLAVATLLSYPLDTLSWDMGHRAHGNWLALWPLLSALSALGALAGAFALG
                     NRGLMGVCVVAALLHAAHFYYAMGTTLMIKSLTMLALGAALLAAAHFLKRAQPR"
     gene            1328..>1735
                     /locus_tag="VF309_06305"
     CDS             1328..>1735
                     /locus_tag="VF309_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HEX7466232.1"
                     /translation="MNITRALILLGAAIVLAAIDVSIMGKENIRHNGEIVYLDLAPRD
                     PRSIMQGDYMALRFRLAQEIEASLHSGAPVDPVLGEGQTGFANIALDEKRVASLAKPG
                     AASNLRIRYRLRKGAVWLGTNAYFFEEGTARRYA"
BASE COUNT          241 a          612 c          580 g          302 t
ORIGIN      
        1 accctcgcct gcatcgtcgc catcgcgcag agcggcgtct tcgccgcgcg cgtgccggcg
       61 acgacgcgcc atgggctcga cgtggtcgcg atcttcgccg tcgcctgcgc cctcatcggc
      121 atcgcgacgg tgcaacggcg catcgacgtg tatccgctgg cgctgatcgc cgcgagcgcc
      181 atcgcggtct cgaccacgct cctcgtccac aacatcaagc tgaacgacct cggcgacttc
      241 ttcgtgatat cgatatggct catcgccacc tcgacggccg cgggcttgat gctgatgcgc
      301 tgggtgcggg cctggcgcac ggatgccgga gcgaacgcat gatcgctacc gcgcaagacc
      361 ttgtgaagga gttgcgcgcc cgcggcatgg tgacggcgca gcccccaatc gcggagagtc
      421 cggccaccgg cggcgaccgc ccgtggtaca tcggcctatt gctcggaacg gccggatggt
      481 tcgcgggcgt cttcgcgctg atcttcgtca tgctcttatc ccggcccacc gcgggctaca
      541 acgcgctcgc ggtcggcatc gtgctcctcg gcgcggcgtg gggaatcttc agggccgatc
      601 gcgagggcgc cttcttctcg cagctggcgc tcgcgctttc gatcgcggga cagttcacga
      661 cgatctacgg catccacgcg ctcttcttca agggcgcgaa cagcatcgcc ggcgtggccg
      721 cgatcgcgtt ggtgctgcag gcgctgctgg tgctcgtgat gcccaaccgg ctgcatcgca
      781 cgatgtcgac cctcttcgcc tgcacggcgt gggcgatcgc ggtgcgctac atcctgtggg
      841 acgagcccgg ccgcaattca caattgccgc cctcgctcgg actggtgctg gtgggcttcg
      901 ccatcgcgtg gctgcccgtg ggcgcgaaac tctacgcgct gatccgcacc gaggcccggt
      961 ggatcgcatc cggccggcag gcgttgctgc ggccggtcac gaccgggctc atcgcgggcc
     1021 tcgccgtggc gacgctcctc tcgtacccgc tcgatacctt gagctgggac atgggccatc
     1081 gcgcgcacgg caactggctc gcgctctggc cgctgctctc ggcgctctcg gccctgggcg
     1141 cgctcgccgg ggccttcgca ttgggaaacc gcggcctcat gggcgtgtgc gtggtggcgg
     1201 cgctgctgca cgccgcgcat ttctactacg cgatgggcac gacgctgatg atcaagtcgt
     1261 tgacgatgct ggcgctgggc gcggcgctgc tggcggcggc gcatttcctg aagcgggcgc
     1321 agccgcgatg aacatcacgc gcgcgctgat cctcctcgga gcggcgatcg tcctcgcggc
     1381 gatcgacgtc tcgatcatgg gaaaggaaaa catcaggcac aacggcgaga tcgtgtacct
     1441 cgacctcgcg cctcgcgatc cgcgctcgat catgcagggc gactacatgg cgctgcgctt
     1501 tcgcctcgcc caggaaatcg aggcgagcct gcattccggg gcgccggtcg acccggtgct
     1561 cggcgaaggc cagaccggct tcgcgaacat cgccctcgac gagaagcgcg tcgcgagcct
     1621 cgccaagccc ggcgcggcgt cgaacctcag gatccgctat cgccttcgca agggcgcggt
     1681 ctggctcggc acgaacgcgt acttcttcga ggaaggcacc gcccgccgct acgcc
//
LOCUS       DASVII010000483         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_274133,
            whole genome shotgun sequence.
ACCESSION   DASVII010000483 DASVII010000000
VERSION     DASVII010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274133"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="VF309_06310"
     CDS             <1..1596
                     /locus_tag="VF309_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7466233.1"
                     /translation="ALSKRLEVRVKREGAVYEMAFSGGEVVEKLRKSGKAGAKETGTW
                     VRAWPDPKYFDTPKVRRQDLEMLLRAKAVLLPGLVVTLEEDGSKDKREWKYEGGLSAY
                     LQERVADAGGYVSPIFAGESHVGADDATYAQGEGATWAIAWAESGGEGESFVNLIPTV
                     LGGTHEAGLRNAVYESIKEFCEHHELLTRGVSLQSEDAWKPLRFVLSAKILDPQFQGQ
                     TKERLSSREAVKLIASRVKDPLDAWLNQNVEEGKKIAQLAISAATARQKAGKVVEKRR
                     GSGVAILPGKLTDCESQDMTRNEVFLVEGDSAGGSAKQGRDRQYQAILPLKGKVLTTF
                     EVDAKELYANTEVHDIAVALGIDPHTREADAKVLDNLRYGKVIIMADADVDGAHIQTL
                     LLTLFYKHFPKLIEEQHVFIAQAPLYAIKVAPQGKAKAERRLYALDESERDQILEKLR
                     DEGVRDSLVHIGRFKGLGEMNPDQLRETTMNPDTRRLIEMRLDPSEYEAAPPVFKLLM
                     GKTEAAGRRAWMEKEGHTIEADL"
BASE COUNT          351 a          574 c          613 g          255 t
ORIGIN      
        1 gcgctctcga agcgcctcga ggtgcgcgtg aagcgcgagg gtgcggtcta cgagatggcg
       61 ttctcgggcg gcgaagtcgt cgagaagctg cgcaagtcgg gcaaggccgg cgccaaggag
      121 acgggcacgt gggtgcgcgc gtggcccgac cccaagtatt tcgacacgcc gaaggtccgc
      181 cgccaggacc tcgagatgct gttgcgcgcg aaggcggtgc tgctgccggg tctcgtcgtg
      241 acgctagagg aggacggctc gaaggacaag cgcgaatgga agtacgaagg cggcctctcc
      301 gcatacctcc aggagcgcgt cgccgacgcc ggcggctacg tgagcccgat tttcgccggc
      361 gaatcccacg tcggcgccga cgacgcgacg tacgcgcagg gagaaggcgc gacctgggcg
      421 atcgcgtggg ccgagtcggg gggcgaaggc gaatcgttcg tgaacctcat tcccaccgtg
      481 ctcggcggta cccacgaggc gggcctgcgc aacgcggtct acgaatccat caaggaattc
      541 tgcgagcacc acgagctcct cacgcgcggc gtgagcctgc aatcggaaga cgcgtggaag
      601 ccgcttcgct tcgtgctctc ggcgaagatc ctcgatccgc agttccaggg ccagaccaag
      661 gagcgcctgt cgtcgcgcga agcggtgaag ctgatcgcct cgcgcgtgaa ggatccgctc
      721 gacgcgtggc tgaaccagaa cgtcgaggaa ggcaagaaga tcgcgcagct cgcgatcagc
      781 gccgcgacgg cgcgccagaa ggccggcaag gtggtcgaga agcggcgcgg ctcgggcgtt
      841 gcgattctcc cgggcaagct caccgattgc gagtcccagg acatgacgag gaacgaagtc
      901 ttcctcgtcg agggcgattc cgcgggcggc tcggccaagc agggacgcga ccgccagtac
      961 caggcgatcc tgccgctgaa gggaaaggtt ctcaccacct tcgaggtgga cgcgaaggag
     1021 ctctacgcga ataccgaagt gcacgacatc gcggtcgcgc tcggcatcga tccgcacacg
     1081 cgcgaggccg atgccaaggt gctcgacaac ctgcgctacg gcaaggtcat catcatggcc
     1141 gacgccgacg tcgacggcgc gcacatccag acgctcctcc tcaccctctt ctacaagcac
     1201 ttcccgaagc tgatcgagga gcagcatgtc ttcatcgcgc aggcgccgct ctatgcgatc
     1261 aaggtcgcgc cccagggcaa ggcgaaggcg gagcggcgcc tctacgccct cgacgagtcc
     1321 gagcgcgacc agatcctcga gaagctccgc gacgagggcg tgcgcgattc gctggtgcac
     1381 atcggccgct tcaagggtct gggcgagatg aaccccgacc agctccggga aaccacgatg
     1441 aacccggaca cgcggcggct catcgagatg cgcctcgatc cctccgaata cgaggcggcg
     1501 ccgccggtgt tcaagctcct catgggcaag acggaagccg cgggccggcg cgcgtggatg
     1561 gagaaggaag gccacacgat cgaggccgac ctgtagtcga cagggtttct ttgcaaggag
     1621 gatccaatgg tatccgcacc ccggttgagg tcattgccgc tcgcgacgat tgcagtgatg
     1681 gcgttgtgcg gcgcttgcgc caccccggca ggggatatcc agccgaccaa cgacctgccc
     1741 aatccgtacc aaaccatcgc accgtgggga gagctccccg aaggcaggaa atg
//
LOCUS       DASVII010000484         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_346183,
            whole genome shotgun sequence.
ACCESSION   DASVII010000484 DASVII010000000
VERSION     DASVII010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346183"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="recO"
                     /locus_tag="VF309_06315"
     CDS             <1..742
                     /gene="recO"
                     /locus_tag="VF309_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7466234.1"
                     /translation="ETSLILQMWTEKHGRLGLVAKGARRPKSPSRSVLVPFQPLSLDW
                     FGRGELRTLKTAEPASPATPLGGSSLLSAFYLNELLLKLTTRDDPHEGLFAAYDDAIA
                     SLRSITRQGQRVAAGVVSEPAAQAARDIEPVLRRFELRLLQELGYAVELTQEARTHAP
                     IEGKGDYWYVAERGPLSAVEGESPANAVRLRGQTLIDLARGNFDDVVTIGQSKQLMRL
                     LIHHSLNGQELATRALVRDLQRLDEEHP"
     gene            739..1488
                     /locus_tag="VF309_06320"
     CDS             739..1488
                     /locus_tag="VF309_06320"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7466235.1"
                     /translation="MIELGVNIDHVATIRQARRTYEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDEDVRRLRDLTHIKLNLEMAATDEMVAIARAIKPEMAMLVPEGRLEVTTE
                     GGLDIVKQEQRLKAVVTKLADAGIVTSVFIDAEPAQVEAAARIGARVCEIHTGPYAHA
                     FHSKGRDAEAKPVLAEVDKVRRAGEAIRSLGMRFNAGHALNYANVEPIAALPGIRELH
                     IGHAIVSRAVFTGMREAVREMKALMTGASAR"
     gene            1485..>1672
                     /gene="acpS"
                     /locus_tag="VF309_06325"
     CDS             1485..>1672
                     /gene="acpS"
                     /locus_tag="VF309_06325"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7466236.1"
                     /translation="MIVGIGTDVCSIERIGRSLERFGDRFVRRILTSDERARFERTRD
                     KAGHLAKRFAAKEAFAKA"
BASE COUNT          289 a          572 c          581 g          230 t
ORIGIN      
        1 cgagacgagc ctcatcctgc agatgtggac ggagaagcat gggcgcctgg gcctggtggc
       61 caagggcgcg cggcggccga agtcgccgag ccgcagcgtg ctcgtcccat tccagccgtt
      121 gtcgctcgac tggttcgggc gcggcgagct gcgcacgctg aagacggcgg aaccggcttc
      181 acccgcgacg cccctcgggg gctccagcct tctttcggcg ttctatctga acgagctcct
      241 cctcaagctc acgacgcgcg acgatccgca cgaggggctc ttcgcggcgt acgacgacgc
      301 catcgcctcg cttcggtcga tcacgcgcca ggggcagcgc gtcgctgcgg gcgtcgtgtc
      361 cgagcccgcg gcccaggcgg cgcgcgacat cgagcccgtg ctgcggcgct tcgagctgcg
      421 cctgctccag gagctgggct atgcggtcga gctcacgcag gaagcccgca cccacgcgcc
      481 gatcgaagga aaaggcgact attggtacgt ggcggaacgc ggacctctct cggcggtcga
      541 gggagagagc ccggcgaatg cggtaagatt gcgcggccaa acgctcatcg acctcgcgcg
      601 cgggaatttc gacgacgtcg tcacgatcgg gcaatccaag cagctcatgc gcctgctcat
      661 ccatcattcc ctgaacggac aggaactcgc cacccgcgcg ctggtgcgcg acctgcagcg
      721 cctggacgag gagcatccgt gatcgagctc ggcgtcaaca tcgaccacgt cgccaccatc
      781 cggcaggcgc gccgcaccta cgagccggac ccggtgtggg cagccgtcga ggcgcacctg
      841 ggcggggccg acggaatcac ggtccacctg cgcgaggacc gccgccacat ccaggacgag
      901 gacgtgcggc ggctgcgcga cctcacgcac atcaagctca acctcgagat ggccgcgacc
      961 gacgagatgg tcgccatcgc gcgcgccatc aagccggaga tggcgatgct cgttcccgag
     1021 ggccgcctcg aggtgacgac cgaaggcggc ctcgacatcg tgaagcagga gcagcgcctc
     1081 aaggctgtcg tcacgaagct cgccgacgcg ggaatcgtga cgagcgtctt catcgatgcc
     1141 gagcctgcgc aggtggaggc cgccgcgcgc atcggcgcgc gcgtctgcga gatccacacg
     1201 ggcccttacg cgcacgcgtt ccattcgaag ggccgcgacg ccgaggcgaa gcccgttcta
     1261 gccgaggtcg acaaggttcg ccgcgccggg gaggcgatcc gctccctcgg gatgcgcttc
     1321 aacgcgggcc acgcgctcaa ctacgcgaac gtcgagccca tcgcggcgct gcctggaatt
     1381 cgcgagctgc acatcgggca cgcgatcgtg agccgggcgg tgttcaccgg aatgcgcgag
     1441 gccgtgcgcg agatgaaagc gctgatgacc ggcgcgagcg cgagatgatc gtgggcatcg
     1501 gaaccgacgt ctgctccatc gagcggatcg gcaggagcct ggagaggttc ggcgatcgct
     1561 tcgtcaggag gatcctcacc tccgacgagc gcgcgcgctt cgagcgcacc cgggacaagg
     1621 ccgggcacct ggcgaagcga ttcgccgcga aggaagcgtt cgccaaggcg at
//
LOCUS       DASVII010000485         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_165958,
            whole genome shotgun sequence.
ACCESSION   DASVII010000485 DASVII010000000
VERSION     DASVII010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165958"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..376
                     /locus_tag="VF309_06330"
     CDS             65..376
                     /locus_tag="VF309_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466237.1"
                     /translation="MKRIILAAALLACATTPFAQGSEVPKSQCDPKPEYPGRLVMQSD
                     SRAKVFNNDVKKYADCMKKYVADRQAATKENSDAANAAIEEYNTVMKKINAAVDEAKQ
                     Q"
     gene            complement(437..1138)
                     /locus_tag="VF309_06335"
     CDS             complement(437..1138)
                     /locus_tag="VF309_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX7466238.1"
                     /translation="MIDLYYWTTPNGHKITMFLEESGLEYGIKPVNIGKGDQFDPAFL
                     KISPNNRIPAIVDHAPAGAGKPISVFESGAILLYLAGKTGQFIPKDLRGQVETLEWLM
                     WQMGGLGPMAGQNHHFAHYAPEKLPYAIDRYVKETSRLYGVLNKRLTSRDFIVGKQYT
                     IADMAAYPWVIPDRQGQDIDDFPNLKRWLAAIRARPATARAYARAKEINPLPRKPHTD
                     EERKILFGQDKSVVR"
     gene            complement(1199..>1898)
                     /locus_tag="VF309_06340"
     CDS             complement(1199..>1898)
                     /locus_tag="VF309_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX7466239.1"
                     /translation="GPSAIPFGHYATPRAMTVHEIERTVEDFRAAAAHALAAGFDVAE
                     IHGAHGYLLHSFASPLSNRREDRYGGSLENRIRLALEVARAVRETWPADKPVFYRISA
                     TDWYEGGWDIEQSVVLCRRLKEIGIDLIDCSSGGNIHDQKIKLGPGYQVPFAEAIRAG
                     AGIPTAAVGLISEAVQAEQIVALGQADAVCMARAFLRDPYWPRHAARALGVELPWPDQ
                     YSRCDVGPLGSPRA"
BASE COUNT          312 a          614 c          654 g          318 t
ORIGIN      
        1 caccgtgccc gtcccggact gaagtacact accgcccgcg atcaatccca ggagctgtcc
       61 acccatgaag cggatcatcc ttgcagcagc gctcctcgcc tgcgcgacca ccccgttcgc
      121 gcaggggagc gaagtaccga agtcccagtg cgaccccaag cccgagtatc ccggccgcct
      181 cgtgatgcag tccgactcgc gcgcgaaggt cttcaacaac gacgtgaaga agtacgcgga
      241 ctgcatgaag aaatacgtgg ccgatcgcca ggccgcgacc aaggaaaaca gcgacgccgc
      301 caacgcggcg atcgaggagt acaacaccgt gatgaagaag atcaacgcgg cggtcgacga
      361 ggcgaagcag cagtaggcgt tcgcgggcgc aggcgcgcgg ccccgatcgg cggcacgtcg
      421 gcgagggttc gccttcctat ctgacgacgg acttgtcctg cccgaagagg atcttgcgct
      481 cttcgtcggt gtgtggcttg cgcggcagcg ggttgatctc cttcgccctc gcgtaggcgc
      541 gtgcggtcgc cgggcgcgcg cggatcgccg cgagccagcg cttgagattc ggaaaatcgt
      601 cgatgtcctg gccctggcgg tcgggaatca cccagggata ggcggccatg tcggcgatcg
      661 tgtactgctt gccgacaatg aagtcgcggc tcgtgaggcg tttgttcaac acgccataaa
      721 gccgagaggt ctccttcacg tagcggtcga tcgcgtacgg aagtttctcg ggcgcgtagt
      781 gggcgaagtg gtggttctgc cccgccatgg gtcccaggcc gcccatctgc cacatgagcc
      841 actcgagcgt ttccacctgc ccacgcaggt ccttcggaat gaattgtccc gtcttgccgg
      901 cgaggtagag gaggatcgcg cccgactcga acacgctgat gggcttgcct gcgcccgcgg
      961 gcgcgtggtc gacgatcgcc gggatgcggt tgttcggcga gatcttcagg aacgcggggt
     1021 cgaactggtc gcccttgccg atgttcacgg gcttgatccc gtactcgagg ccggattcct
     1081 cgaggaacat cgtgatcttg tgcccgttgg gcgtcgtcca gtaatagaga tcgatcatcg
     1141 ggcctgcgct cctccgacgt tggacggttt tctcacgggg accttgcgtg gggagccatt
     1201 atgcccgagg ggacccgagc ggccccacgt cgcagcggct gtattgatcg ggccacggga
     1261 gctccacgcc gagcgccctc gcggcatggc gcggccagta cggatcgcga aggaacgcgc
     1321 gcgccatgca caccgcgtcg gcctgtccga gcgccacgat ctgctcggcc tgcacggcct
     1381 cggagatgag gccgacggcg gccgtgggaa tgccggcgcc cgcgcggatc gcctcggcga
     1441 acggcacctg gtagcccggc ccgagcttga tcttctggtc gtggatgttg cctccgctcg
     1501 agcagtcgat gaggtcgatg ccgatctcct tcagccgcct gcacaggacc accgactgct
     1561 cgatgtccca gccgccctcg taccagtcgg tggcggagat gcgatagaag acgggcttgt
     1621 cggcgggcca cgtctcgcgc accgcgcgcg ccacctcgag cgccaggcgg atgcgattct
     1681 ccagcgagcc gccatagcgg tcttcgcggc ggttcgacag gggcgaggcg aacgaatgca
     1741 ggaggtagcc gtgggcgccg tggatctcgg cgacgtcgaa tccggcggcg agcgcgtgag
     1801 ccgcggccgc gcggaagtcc tcgacggtgc gctcgatctc gtgcaccgtc atcgcgcggg
     1861 gggtcgcgta gtggccgaag gggatcgcgc tgggcccg
//
LOCUS       DASVII010000486         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_238478,
            whole genome shotgun sequence.
ACCESSION   DASVII010000486 DASVII010000000
VERSION     DASVII010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238478"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VF309_06345"
     CDS             <1..719
                     /locus_tag="VF309_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7466240.1"
                     /translation="GFNTTKGVTKDKRVRQALARAIDRQKIVKFITRAGEVAAESLVP
                     PGMPGYEPAKAGPFDPEAARRLLADAGYPGGKGFPKIEILYNTQDVHKKIAAAIQEMW
                     REHLHIESELRNVEWKIYLDMQTRMEFDVIRRAWIADYNDPNTFLDMFTSANGNNNTG
                     FANEAYDKLLAAAAKELDPVKRMKLLHGAEAILMEELPIVPMYFYVTKNMWKPEVTGI
                     YDNVRDTHPYNRIRLAEKPR"
     gene            719..1654
                     /locus_tag="VF309_06350"
     CDS             719..1654
                     /locus_tag="VF309_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7466241.1"
                     /translation="MIAFFLKRLAGGALTLFVIATLCFFIIRFAPGNPFTGEKKLPPE
                     VLRNLERAYNMDKPLIVQYGLRMKGYLSGDLGLSTKYLGKRVDELLFPSFGTSLQLGA
                     LGFVFAIFFGVPVGVLASAKQNRLPDYLSSSAALAGICIPNFLLGPILVMIFALTLKW
                     LPVAGWPEDFSGEELLKLLLPAVTLGAVHVAYVSRLTRAGMLDVLHRDYIRTARAKGV
                     GERSVILKHALKNGITPVLSYAGPMAAYIFTGSVVVEKIFNLPGMGQHFVDAALARDD
                     AMVMGAVLIYSVLVIAFNLVVDLGYSVLDPRVRLT"
     gene            1651..>2235
                     /locus_tag="VF309_06355"
     CDS             1651..>2235
                     /locus_tag="VF309_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7466242.1"
                     /translation="MSASSSPTQLALRRFTKNRRAMACFWIMLTLGISTLAVPLLSPH
                     DYRTQKLELQKKPPNGTYWMGTDPNGRDLMVRTFMGGRVSFAVGLLATGISLLIGVAY
                     GAVAGFLSGKVDAWLMRFVDVLYGLPHMFLVIIVMSILGRNFLVVFMVLGCFSWMTMS
                     RIVRGQVLSLKEKEFVEAARALGVSTPAIIARHMI"
BASE COUNT          382 a          797 c          658 g          398 t
ORIGIN      
        1 acggcttcaa tacgaccaag ggcgtgacga aggacaagcg cgtccgccag gcgctggcgc
       61 gggccatcga ccgccagaag atcgtgaagt tcatcacgcg cgcgggcgaa gtcgccgcgg
      121 agtcgctggt gcctccgggg atgccgggct acgagcccgc gaaggccggc cccttcgatc
      181 ccgaagccgc ccgccggctc ctcgccgacg cgggatatcc ggggggcaag ggcttcccga
      241 agatcgagat cctctataac acgcaggacg tgcataagaa aatcgccgcc gccatccagg
      301 agatgtggcg agagcacctc cacatcgagt ccgagctgcg caacgtcgag tggaagatct
      361 acctcgacat gcagacgcgg atggagttcg acgtgatccg ccgcgcctgg atcgccgact
      421 ataacgaccc caataccttc ctcgacatgt tcacgtcggc gaacggaaac aacaacacgg
      481 gcttcgcgaa cgaggcctac gacaagctct tggcggcggc ggcgaaggaa ctggatcccg
      541 tcaagcgcat gaagctgctc cacggcgccg aagcgatcct gatggaggag ctcccgatcg
      601 tcccgatgta cttctacgtc accaagaaca tgtggaagcc cgaggtcacg ggcatctacg
      661 acaacgtccg cgacacgcac ccctacaacc ggatccgcct ggcggagaag ccccggtaat
      721 gatcgccttc ttcctcaagc gcttggccgg cggcgcgctc acccttttcg tcatcgcgac
      781 gctttgcttc ttcatcatcc gcttcgcgcc cggcaatccc ttcacgggcg agaagaagct
      841 gccgcccgag gtcctccgca acctcgaacg cgcctacaac atggacaagc cgctgatcgt
      901 ccagtacggc ctgcgcatga agggctacct gagcggcgac ctggggctgt ccaccaagta
      961 cctcggcaag cgggtcgatg aattgctctt cccctccttc ggtacctcac tccagctggg
     1021 cgccctcgga ttcgtcttcg cgatcttctt cggcgtcccc gtcggcgtcc tcgcgtccgc
     1081 gaagcagaac cgcctgcccg actatctctc ctcgagcgcc gcgcttgccg gcatctgcat
     1141 ccccaatttc ctcctcggcc ccatcctcgt gatgatcttc gcgctcacgc tgaagtggct
     1201 cccggtcgcg ggctggcccg aggacttcag cggcgaagaa ctgctcaagc tgctgctccc
     1261 cgccgtcacg ctgggcgcgg tccacgtcgc ctacgtctcc cgcctcaccc gggcggggat
     1321 gctcgacgtc ctccatcgcg actatatccg caccgcgcgg gccaagggcg tcggcgagcg
     1381 ctccgtcatc ctgaagcacg cgctcaagaa cggcatcacc cccgtgctct cctacgccgg
     1441 ccccatggct gcctacatct tcaccggctc ggtcgtcgtc gagaagatct tcaacctgcc
     1501 cggcatgggg cagcacttcg tcgacgccgc cctcgcccgc gacgacgcca tggtcatggg
     1561 cgcggtcctc atctacagtg tcctcgtcat cgccttcaac ctggtcgtcg acctgggcta
     1621 ttccgtcctc gatccgcggg tgaggctgac atgagcgcga gcagcagccc gacgcagctc
     1681 gcgctgcgcc gcttcacaaa gaaccgccgg gccatggctt gcttctggat catgctcacg
     1741 ctggggatct cgacgctcgc ggtccccctc ctctctccgc acgactaccg cacccagaag
     1801 ctggagctgc agaagaagcc gcccaacggc acttattgga tgggcacgga ccccaacggc
     1861 cgcgacctca tggtccgcac cttcatgggc ggacgcgttt ccttcgcggt cggcctcctc
     1921 gcgaccggca tcagtctgct gatcggcgtg gcctacggcg cggtcgccgg cttcctgagc
     1981 ggcaaggtcg acgcctggct gatgcgcttc gtcgacgtcc tctacggcct gccgcacatg
     2041 ttcctggtca tcatcgtcat gtccatcctg ggccggaact tcctcgtcgt cttcatggtg
     2101 ctgggctgct tcagctggat gacgatgtcg cgcatcgtgc gcggccaggt gctgagcctg
     2161 aaggaaaagg agttcgtgga ggcggcccgc gcgctggggg tatcgacgcc ggcgatcatc
     2221 gcgcgccaca tgatc
//
LOCUS       DASVII010000487         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_418121,
            whole genome shotgun sequence.
ACCESSION   DASVII010000487 DASVII010000000
VERSION     DASVII010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418121"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /gene="purL"
                     /locus_tag="VF309_06360"
                     /gene_synonym="purI"
     CDS             <1..1827
                     /gene="purL"
                     /locus_tag="VF309_06360"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX7466243.1"
                     /translation="GEAVTNLAAAPIGPLSRIKLSCNWMAAAGHPGEDARLYAAVRAA
                     SEIAIALGIAIPVGKDSMSMRTTWRDAKSGQEKSVTAPLSLIVSAFAPVSDVRGTLTP
                     ELRMDAGDTELVLVDLGAGANRLGGSALAQVHSQLGDEAPDLDDPAKLKAFFGAVQRL
                     RGEGRLLAYHDRSDGGLLATLCEMAFAARAGVSVYLDTLALDPRQLDVDGHERETGLL
                     AGSLHERILATLFNEELGAVLQVRRGDRDAVMAVLREAGLSADSHVIGHPNADGEVRF
                     IVSGKAVFAEKRASLHRAWSEVTHHIQRLRDNPACADQEYERNLDEGDPGLNAKLTFD
                     PAEDIAAPHIGAPSVSRAARPRIAVLREQGVNGQVEMAAAFDRAGFAAVDVHMSDIIA
                     GRVSLAAFKGFAACGGFSYGDVLGTGEGWAKSILFNPRARDEFEAFFRRSDSFALGAC
                     NGCQMMSNLKDIIPGARNWPHFERNASEQYEARVALIEIQKSPSILFPGMEGSRIPIA
                     TAHGEGRAVFKGEAAMDAARFLVAARFVDNRGKVTETYPYNPNGSPQGVTALTTPDGR
                     FNILMPHPERVFRSILLSWHPREWGEDSPWMRMFRNARAWVD"
BASE COUNT          294 a          669 c          683 g          288 t
ORIGIN      
        1 ggcgaggcag tcacgaacct tgctgccgcg ccgatcggtc cgctgtcgcg tatcaagctg
       61 tcgtgcaact ggatggcggc cgccggtcac cccggcgagg acgcgcgtct gtacgcggcc
      121 gtccgcgccg cgagcgagat cgcgatcgcg ctcgggatcg cgatccccgt cggcaaggat
      181 tccatgtcga tgcgcaccac ctggcgcgat gcgaagagcg gccaggaaaa gtccgtcacg
      241 gcgccgctct cgctcatcgt ctccgcgttc gctccggtga gcgacgtgcg cgggacgctc
      301 acgccggagc tgcgcatgga cgcgggggac acggagctcg tgctggtcga cctcggcgcg
      361 ggcgcgaacc gcctcggcgg ctcggcgctc gcgcaggtgc actcgcagct cggcgacgag
      421 gcgcccgatc tcgacgatcc ggcgaagctg aaggccttct tcggcgccgt gcaacgcctg
      481 cgcggggaag ggcgcctgct cgcctatcac gaccgttccg acggcgggct cctcgcgacg
      541 ctttgcgaga tggcgttcgc cgcgcgcgcc ggggtgagcg tgtatctcga tacgctcgcg
      601 ctcgatccgc ggcagctcga cgtcgacggc cacgagcgcg agaccgggct cctcgcgggc
      661 agcctccacg agcgcatcct cgcgacgctc ttcaacgagg agctgggcgc cgtgctgcag
      721 gtccgccgcg gggaccgcga cgcggtgatg gcggtcctgc gcgaggcggg cctttccgcc
      781 gattcccacg tgatcgggca cccgaatgcc gacggcgagg tgcgcttcat cgtgagcggc
      841 aaagccgtgt tcgccgagaa gcgcgcgtcg ttgcatcgcg cgtggtcgga ggtgacgcac
      901 cacatccagc gcctgcggga caaccccgcg tgcgccgacc aggagtacga gcgcaacctc
      961 gacgagggcg atccggggct caacgcgaag ctcaccttcg atcccgcgga ggacatcgcg
     1021 gcgccgcaca tcggagcgcc gagcgtttcg agggccgcgc gcccgcgcat cgcggtgctg
     1081 cgcgagcagg gggtgaacgg ccaggtggag atggccgcgg ccttcgatcg cgccggattc
     1141 gccgcggtcg acgtgcacat gagcgacatc atcgccggcc gcgtctcgct cgcggccttc
     1201 aagggcttcg cggcgtgcgg cggcttcagc tacggagacg tgctgggcac gggcgagggc
     1261 tgggccaagt cgatcctctt caaccccagg gcacgcgacg agttcgaggc gttcttccgg
     1321 cgctccgact cgttcgcgct cggcgcctgc aacggctgcc agatgatgtc gaacctgaag
     1381 gacatcattc ccggggcgcg caactggccg cacttcgagc gcaacgcctc ggagcaatac
     1441 gaggcgcgcg tcgcgttgat cgagatccag aagtcgccct cgatcctctt cccgggaatg
     1501 gaaggaagcc gcattcccat cgcgacggcg cacggagagg gccgcgcggt gttcaagggc
     1561 gaagccgcga tggacgcggc ccgcttcctg gtcgcggcgc gcttcgtcga caaccgcggc
     1621 aaggtcaccg agacctatcc gtacaacccg aacggctcgc cgcaaggcgt gaccgccctc
     1681 accacgcccg acgggcgctt caatatcctc atgccccatc cggagcgcgt gttccgctcg
     1741 atcctcctgt cgtggcaccc gcgcgaatgg ggcgaggatt cgccgtggat gcgcatgttc
     1801 cgcaacgccc gcgcctgggt ggattgatcg cggccgcgac gtcgatgccg gccgccgggc
     1861 gcaagctccg agcaaggaaa agcggcttat gatcgggtag ggggctatga ttgcagcgcc
     1921 ccaagatggg tttc
//
LOCUS       DASVII010000488         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_23098, whole
            genome shotgun sequence.
ACCESSION   DASVII010000488 DASVII010000000
VERSION     DASVII010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23098"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF309_06365"
     CDS             complement(<1..244)
                     /locus_tag="VF309_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466244.1"
                     /translation="MSVDAKRRRMLGASTGIFVCAALGAGLAARGEDKVIRVAARKFV
                     FTPNEIALKKGEPVVIELSTLDVFMGFNVPELKVRSD"
     gene            complement(241..1122)
                     /locus_tag="VF309_06370"
     CDS             complement(241..1122)
                     /locus_tag="VF309_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7466245.1"
                     /translation="MTIDRRQFLKLSALGGGAVFASSLAGCASMGAGEPDFYFVQLSD
                     THWGFSGPAVNPDARGTLPKAIAAVNALSVEPDFIVFTGDLTHTTDDPVERRKRLAEF
                     KQIASGLKNKTVRFMPGEHDASLDNGAAWKEFFGETHYTFDHKGVHFIAVDNVSDPAA
                     RIGEEQLAWLKADLARQPKDARIVVLTHRPLFDLAPEWDWATRDGAQAIDMLMPFSNV
                     TVFYGHIHQENHQMTGHIAHHSAKSLIFPLPAPKSQPKRDPIKWDAAAPYRGLGFREV
                     EAEASKYDIKEMPVVKA"
     gene            1257..>1677
                     /locus_tag="VF309_06375"
     CDS             1257..>1677
                     /locus_tag="VF309_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7466246.1"
                     /translation="MTRLARFEKSVLPHLDAAYNLARWLTRNDHDAEDVVQEAYLRAF
                     RFFDGMRAGDARPWLLAIVRNAGYSWLEKNRPAEVVALDDAGLIASDVETAGHAAPAE
                     SNPEVIMLQSANRKLVNQALEEIPVGYREVVVMRELED"
BASE COUNT          278 a          530 c          576 g          293 t
ORIGIN      
        1 tgtcgctcct caccttgagc tccggcacat tgaatcccat gaagacgtcc agggtggaga
       61 gctcgatcac cacgggctcg cctttcttca acgcgatctc gttcggcgtg aagacgaact
      121 tgcgcgcggc gacgcggatg accttgtctt cgccgcgcgc ggccagtccc gcgccgagcg
      181 cggcgcaaac gaagattccc gtcgacgctc ccagcatcct ccgccgcttc gcgtccacgc
      241 tcatgccttc accaccggca tttccttgat gtcgtacttg gaggcctcgg cctcgacctc
      301 gcggaatccc aggccgcggt agggagcggc agcgtcccac ttgatcggat cgcgtttcgg
      361 ctgggacttg ggcgcgggga gcggaaagat gagcgacttc gcggaatggt gcgcgatgtg
      421 gcccgtcatc tggtgattct cctggtggat gtggccgtag aaaacggtca cgttcgagaa
      481 gggcatgagc atgtcgatcg cctgcgcgcc gtcgcgcgtc gcccagtccc actcgggcgc
      541 gaggtcgaag agcgggcgat gggtgaggac cacgatccgc gcgtccttgg gctggcgcgc
      601 gaggtcggcc ttgagccatg cgagctgctc ctcgccgatg cgcgccgcag gatcggagac
      661 gttgtcgacc gcgatgaagt gcacgccctt gtggtcgaag gtgtagtggg tctcgccgaa
      721 gaattccttc cacgcggcgc cgttgtcgag ggaagcgtcg tgctccccgg gcatgaagcg
      781 cacggtcttg ttcttgagcc ccgaggcgat ctgcttgaat tccgcgaggc gcttgcgccg
      841 ctccacggga tcgtcggtgg tatgggtgag gtccccggtg aatacgatga agtcgggctc
      901 gacggaaagc gcgttcaccg cggcgatcgc cttcggcagc gtgccgcggg cgtcggggtt
      961 caccgcgggt ccgctgaagc cccaatgggt gtcggagagc tgcacgaagt agaagtcggg
     1021 ctcgccggcg cccatcgagg cgcagccggc aaggctcgac gcgaagacgg caccgcctcc
     1081 aagggcgctc agcttgagaa attgcctgcg atcgatcgtc atggggtgtt tcctctcggg
     1141 ttgggttttc gggctggcac atgcaatacc ggcgaacccc ccgcgttatt ccgaccataa
     1201 tgcgaacgcg gcgggaataa acgcccgtcc gcagcggtca tcactatgga atgcccatga
     1261 ctcgccttgc gcggttcgag aagagcgtgc tgccgcacct ggatgccgcg tacaacctcg
     1321 cgcggtggct cacgcgaaac gaccacgacg ccgaagacgt cgtgcaggag gcgtacttgc
     1381 gcgcgttccg gttcttcgac ggcatgcgcg cgggcgacgc gcgcccgtgg ctcctcgcga
     1441 tcgtgaggaa cgcgggctat tcgtggctcg agaagaaccg ccccgcggaa gtggtggcgc
     1501 tcgacgacgc gggcctcatc gcctcggacg tcgagaccgc gggccacgcc gcgccggcgg
     1561 aaagcaatcc ggaagtgatc atgctgcaga gcgcgaaccg caagctcgtg aaccaggcgc
     1621 tcgaggagat ccccgtcggc taccgcgagg tcgtcgtgat gcgcgagctg gaagacc
//
LOCUS       DASVII010000489         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_238629,
            whole genome shotgun sequence.
ACCESSION   DASVII010000489 DASVII010000000
VERSION     DASVII010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238629"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VF309_06380"
     CDS             <1..878
                     /locus_tag="VF309_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466247.1"
                     /translation="GGIMPAPILMVSDSRLVGFANPWVRPPTAVYSQVALTNFRGVDL
                     LVRQAAGDVNLTAQPYFGKAPTQIPNAGSDHLTAQIDNMAGLNLGAELGGWTARVGYM
                     QAKFTYHSASIDQLFAGVRSVNAFVPGAAALADSLDATGKRIRFMSAGAAYEGGNLLF
                     QAEYAKRKADFFLGETTSWYTTLGYRHGDLMPYVTLSQVKMDSPSSDSTIPPVGPLAA
                     LSGGVNTLLAGQNTAQKVVALGMRYQFAKNADVKVQWDRVRIPQGSTGGFQHPTPDFT
                     GGNVNVYSATVDFVF"
     gene            893..1321
                     /locus_tag="VF309_06385"
     CDS             893..1321
                     /locus_tag="VF309_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7466248.1"
                     /translation="MKNNWLSLIAVATATVLAWPASAELVVIVNPKNPATSLSADQVA
                     QLFLGKSSSFPGGAPAVPLNLSEGAALRDEFHSKVADKNPGQVKAYWAKQMFSGKGSP
                     PRELPSAAEVKKAVASDPSAIGYIDKGTLDSTVKSVLTVQ"
     gene            1335..>2141
                     /locus_tag="VF309_06390"
     CDS             1335..>2141
                     /locus_tag="VF309_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466249.1"
                     /translation="MNFGVRKQIWSLPAIAVLIFGIGIAVSLAYSSRALALIDHVSVV
                     DYPLLEKVAALSSAVQRITDDFNAAVSEGERTKLDEARADIGRVKELLQQIGRIPGEA
                     EFARRVGDEFDAYRAPADSVARIMLGIETGDAKPGVATMQAAIRLLNDDLDKATQRAS
                     SQFAANLAATEENIGNLLRAIIGAAVAVVLLLMLVSHIIVKAIWRQLGGEPEYATRIA
                     RAIARGDLATPIDLDRGDTTSQLAALKEMQGSLASLIENIRIAAGSVRGVA"
BASE COUNT          373 a          734 c          709 g          325 t
ORIGIN      
        1 gcggcggcat catgcccgcg ccgatcctca tggtctccga ctcgcgcctg gtcgggttcg
       61 ccaacccctg ggtgcgcccc ccgacggccg tctactcgca agtggcgctc accaactttc
      121 gcggcgtgga tctcctcgtt cgccaggcgg cgggcgacgt caatctcacc gcgcagccgt
      181 atttcggcaa ggcgcccacg cagattccca atgccggaag cgatcacctc accgcgcaaa
      241 tcgacaacat ggccggcctc aacctcggcg ccgagctggg cggctggacc gcgcgcgtgg
      301 gatacatgca ggcgaagttc acctatcaca gcgcctcgat cgaccagctc ttcgcgggcg
      361 tgcgcagcgt gaacgcgttc gtgccggggg ccgcggcgct cgcggacagc ctcgacgcga
      421 ccggcaagag gatcaggttc atgtcggcgg gcgcggccta cgagggcggc aacctgctct
      481 tccaggcgga atacgcgaag cgcaaggcgg acttcttcct cggcgagacc acatcgtggt
      541 acacgacgct gggatatcgc cacggcgatc tcatgccgta cgtgacgctc tcccaggtga
      601 agatggacag cccgagctcc gactcgacga ttccgcccgt gggacccctc gccgcgctgt
      661 cgggcggggt gaacacgctc ctcgccgggc agaacaccgc gcagaaggtg gtcgcgctcg
      721 gcatgcgcta ccagttcgcc aagaacgccg acgtgaaagt gcaatgggac cgcgtgcgga
      781 ttccgcaagg ctcgaccggc ggcttccagc atcccacgcc cgatttcacc gggggcaacg
      841 tgaacgtgta cagcgccacc gtcgacttcg tattctgagc aaggggacac tcatgaagaa
      901 caactggctc agcctcatcg ccgtcgccac ggcaaccgtc ctcgcatggc ccgcgtccgc
      961 ggagctcgtg gtgatcgtca atccgaagaa tcccgcgact tcgctctcgg cggaccaggt
     1021 ggcgcaactc ttcctgggca agtcgagctc cttcccgggc ggcgcgcccg cggtgccgct
     1081 caacctctcc gagggcgcgg cgctgcgcga cgaattccac agcaaggtgg ccgacaagaa
     1141 tccggggcag gtgaaggcct actgggcgaa gcagatgttt tccggaaagg gctcgccgcc
     1201 gcgcgagctc ccgtccgccg cggaagtgaa gaaagccgtg gcgagcgatc catccgccat
     1261 cggctacatc gacaagggca ctctcgattc aaccgtgaag agcgtgctca ccgtccagta
     1321 accgcgccgc cgacatgaac ttcggggtca ggaagcagat ctggtcgctg ccggcgatcg
     1381 ccgtgctgat cttcggcatc ggcatcgcgg tgagcctcgc ttactcttcg cgagctctcg
     1441 cgctcatcga tcacgtgagc gtggtcgact atccgctgct ggagaaggtg gcggccctct
     1501 cgagcgcggt gcagcggatc accgacgact tcaatgcggc cgtgtccgag ggcgagcgga
     1561 cgaagctcga cgaggcgcgg gccgacatcg ggcgggtgaa ggagctgctg cagcagatcg
     1621 gccggattcc cggcgaagcc gaattcgctc gccgcgtggg cgacgagttc gacgcctatc
     1681 gcgctcccgc cgattccgtc gcgcgcatca tgctcgggat cgagacgggc gacgccaagc
     1741 cgggcgtggc cacgatgcag gccgcgatcc ggctcctcaa cgacgatctc gacaaggcca
     1801 cgcagcgcgc gagctcgcag ttcgcggcga acctcgcggc gaccgaggag aacatcggca
     1861 acctgctacg cgccatcatc ggcgcggcgg tcgcggtcgt gctgctgctc atgctggtgt
     1921 cgcacatcat cgtgaaggcg atctggcgcc agctcggagg cgagccggag tacgcgacgc
     1981 gcatcgcgag ggccatcgcg cggggcgacc ttgcgacgcc catcgacctg gaccgcggcg
     2041 acacgacgag ccagctcgcg gcgctcaagg aaatgcaggg ctcgctcgcg agcctcatcg
     2101 agaacatccg catcgccgcg ggatcggtgc gcggcgtggc t
//
LOCUS       DASVII010000490         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_274329,
            whole genome shotgun sequence.
ACCESSION   DASVII010000490 DASVII010000000
VERSION     DASVII010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274329"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..>2365
                     /locus_tag="VF309_06395"
     CDS             7..>2365
                     /locus_tag="VF309_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7466250.1"
                     /translation="MPVEFKLNGKDVIGYGNETIIQTAKRHDIEIPHLCYKEGMRPDG
                     NCRACVVEIKGERVLAPSCCRQPAKGMEVSSNNARAVHSQKMVVELLMSDVPKEDLKP
                     GGNELFQWAGELGVTRSRFAPRHQPKSDHSHPAMSVNLDACIQCTRCVRACREEQVND
                     VIGYASRGLDSKIVFDFDDPMGESTCVACGECVQACPTGALAPAKNAYQVLADRKIDS
                     VCPYCGVGCQLTYHVKDNKIARVEGRDGPSNHERLCVKGRFGFDYVQHPHRLTKPLIR
                     KPGAKKTGEFTVDPTRWSDIFREATWEEALDLAANGLKKVRDTKGPNALAGFGSAKGS
                     NEEAYLFQKLVRTGFGTNNVDHCTRLCHASSVVALLEGIGSGAVSNQVSDVAKAELIF
                     VIGANPTSNHPVAATWMKNAVDRGAKLVLADPRRNDLARHAWRFLQFQADTDVAMLNA
                     MIYTIIEEGLVDQEFVRQRTTNYEALKENAKDFSPELMAPICGIPAETLREVARAYAT
                     SKASIIMWGMGISQHVHGTDNARCLIALSLMTGQIGRPGTGLHPLRGQNNVQGASDAG
                     LIPMMFPDYQRVDNPEANARFEKFWGRTLDKKPGLTVVEIMDAACDGKMKGMYIMGEN
                     PAMSDPNAHHAREGLASLDCLVVQDIFLTETAYLADVVLPASAWPEKTGTVSNTDRMV
                     QLGRRALDMPGDARQDLWIIQQLANRLGCGWSYTGAESGVAEVYDEMRRAMPTITGIT
                     WERLQEQGSVTYPCRTEEDPGQPVVFTENFPTKDGRGKFVPADIIP"
BASE COUNT          451 a          811 c          764 g          339 t
ORIGIN      
        1 gaggcgatgc cggtcgagtt caagctgaac ggcaaggacg tgatcggcta cggcaatgag
       61 acgatcatcc agaccgccaa gcgccacgac atcgaaatcc cgcacctgtg ctacaaggaa
      121 ggcatgcggc ccgacggcaa ctgccgcgcg tgcgtggtcg agatcaaggg cgagcgcgtc
      181 ctggcgccct cgtgctgccg ccaacccgcc aagggcatgg aagtctcgag caacaacgcg
      241 cgcgccgtgc attcgcagaa gatggtggtc gagctcctca tgtccgacgt gccgaaggag
      301 gacttgaagc ccggcggcaa cgagctcttc cagtgggcgg gcgagctcgg cgtcacgcgc
      361 tcgcgtttcg cgccgcgcca ccagccgaag tccgaccatt cgcacccggc gatgagcgtc
      421 aacctcgacg cctgcatcca gtgcacgcgc tgcgtgcgcg cctgccgcga ggagcaggtg
      481 aacgacgtga tcggctacgc cagccgcggc ctcgattcga agatcgtgtt cgacttcgac
      541 gatccgatgg gcgagtccac ctgcgtcgcg tgcggcgaat gcgtgcaggc ctgtccgacg
      601 ggcgcgctcg ctccagcgaa gaacgcgtac caggtcctcg ccgacaggaa gatcgattcc
      661 gtctgcccgt actgcggcgt gggctgccag ctcacctatc acgtgaagga caacaagatc
      721 gcgcgcgtcg aggggcgcga cggcccctcg aaccacgagc gactgtgcgt gaagggccgc
      781 ttcggcttcg actacgtgca gcacccgcac cggctcacca agccgctcat ccgcaagccc
      841 ggggcgaaga agaccggcga attcaccgtc gatcccacgc gctggagcga catcttccgc
      901 gaggcgacgt gggaggaagc gctcgacctc gccgccaacg gcctgaagaa ggtccgcgat
      961 acgaaggggc cgaacgcgct cgccggcttc ggcagcgcca agggctccaa cgaggaggcc
     1021 tacctcttcc agaagctggt gcgcaccggc ttcggcacca acaacgtcga ccactgcacg
     1081 cggctatgcc atgcatcgtc ggtggtggcg ctcctcgaag gcatcggctc gggcgccgtg
     1141 tccaaccagg tctccgacgt cgccaaggcc gagctcatct tcgtgatcgg cgccaatccg
     1201 acctcgaacc atccggtggc cgcgacctgg atgaagaacg ccgtggaccg cggcgccaag
     1261 ctggtcctcg ccgacccgcg caggaacgac ctggcgcgcc acgcgtggcg cttcctgcag
     1321 ttccaggccg ataccgacgt cgcgatgctg aacgcgatga tttacacgat catcgaggag
     1381 ggcctcgtcg accaggagtt cgtgcgccag cgcaccacca actacgaggc cctcaaggaa
     1441 aacgcgaagg atttcagccc cgagctgatg gcgcccatct gcggcattcc cgcggagacg
     1501 ctgcgcgagg tggcgcgcgc ctacgccacg tcgaaggcct cgatcatcat gtggggcatg
     1561 ggcatctcgc agcacgtgca cggcaccgac aacgcgcgct gcctcatcgc gctctcgctc
     1621 atgaccgggc agatcggccg ccccggcacg gggctgcatc ccctgcgcgg ccagaacaac
     1681 gtgcagggcg cttccgacgc ggggctcatc ccgatgatgt ttcccgacta ccagcgcgtc
     1741 gacaatccgg aagccaacgc gcgcttcgag aaattctggg ggcgcacgct cgacaagaag
     1801 ccgggcctca ccgtggtcga gatcatggac gccgcgtgcg atgggaaaat gaagggcatg
     1861 tacatcatgg gcgagaaccc cgcgatgtcc gatcccaacg cccaccacgc acgcgaaggg
     1921 ctcgcgagcc tcgattgcct cgtggtgcag gacatcttcc tcaccgagac cgcatacctc
     1981 gccgatgtcg tgctgcctgc ctcggcgtgg cccgagaaga ccggcaccgt ctccaacacc
     2041 gaccgcatgg tgcagctcgg gcggcgcgcg ctcgacatgc ccggcgacgc gcgccaggac
     2101 ctgtggatca tccagcagct cgccaatcga ttgggctgcg ggtggagcta caccggcgcc
     2161 gagagcggcg tcgcggaagt ctacgacgag atgcggcgcg cgatgcccac gatcaccggc
     2221 attacctggg agcgcctgca agagcagggc tcggtcacgt atccgtgtcg aacggaagaa
     2281 gatccgggac agccggtggt gttcacggaa aacttcccga ccaaggacgg gcgcggcaag
     2341 ttcgtgcccg ccgacatcat tcccg
//
LOCUS       DASVII010000491         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_238800,
            whole genome shotgun sequence.
ACCESSION   DASVII010000491 DASVII010000000
VERSION     DASVII010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238800"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1598
                     /gene="rpoB"
                     /locus_tag="VF309_06400"
     CDS             <1..>1598
                     /gene="rpoB"
                     /locus_tag="VF309_06400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881213.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7466251.1"
                     /translation="IGLVRMERAIKEKMSVYQEMATAMPHDLINAKPVMAAIREFFGS
                     SQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTHYGRICPIETPEG
                     PNIGLISSLSCYARINEFGFIESPYRKVKDGRVTDYCIVTNAGGSKYKAGDIVEADEM
                     VNEEGRSKKKGVEVEPYSFYLSAWEEDQYIIAQANAAVDNDGKLTQDRVNARQAGNFI
                     LSPKANIQFIDVSPKQLVSVAASLIPFLENDDANRALMGSNMQRQAVPLLRARAPFVG
                     TGMEYITARDSGAVIVARRSGTIDYVDSQRIVVRVESENEDVSKEMGADIYPMTKFKR
                     SNQNTCITQKPIVRVGQKVHKGQVLGDGPCTELGELALGRNVLVAFMPWRGYNFEDAI
                     LVSERMVKDDYYTSIHIEEFEIESRDTKLGPEEITRDIPNVSEGFLKDLDESGIIRIG
                     ANVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRDASLICPPGIEGIIVGVK
                     IFSRKGIEKDDRAKAIEQDELDMM"
BASE COUNT          336 a          497 c          529 g          236 t
ORIGIN      
        1 gcatcgggct ggtccgcatg gagcgcgcga tcaaggaaaa gatgtcggtc taccaggaaa
       61 tggcgaccgc catgccgcac gacctgatca acgcgaagcc ggtcatggcg gcgattcgcg
      121 agttcttcgg gtcgtcgcag ctgtcgcagt tcatggacca gaccaacccg ctctccgaga
      181 tcacccacaa gcggcgtctc tcggcgctcg ggccgggcgg tttgtcgcgc gagcgcgcgg
      241 gcttcgaagt ccgcgacgtc cacccgacgc actacgggcg catctgcccg attgaaacgc
      301 cggaaggccc gaacatcggc ctcatctcgt cgctgtcgtg ctacgcgcgg atcaacgagt
      361 tcggcttcat cgagtcgccg taccggaagg tcaaggacgg ccgcgtcacc gactactgca
      421 tcgtcaccaa cgcgggcggg tcgaagtaca aggcgggcga catcgtcgaa gccgacgaga
      481 tggtgaacga agaggggcgg tcgaagaaga agggcgtgga agtcgagccc tattccttct
      541 acctctcggc gtgggaagag gaccagtaca tcatcgcgca ggcgaacgcg gccgtggaca
      601 acgacggcaa gctgacgcag gaccgcgtca acgcgcggca ggcgggcaac ttcatcctgt
      661 cgcctaaggc gaacatccag ttcatcgacg tgtcgccgaa acagctcgtc tcggtggccg
      721 cgtcgctgat cccgttcctc gagaacgacg atgcgaaccg cgcgctgatg ggatcgaaca
      781 tgcagcgcca ggcggtgccg ctgctccgcg cccgggcgcc gtttgtcggc accggcatgg
      841 agtacatcac ggcgcgcgac tcgggcgccg tcatcgtcgc gcggcgatcg ggcaccatcg
      901 actacgtcga ctcgcagcgc atcgtcgtgc gcgtggaaag cgagaacgaa gacgtgagca
      961 aggagatggg cgccgatatc tacccgatga cgaaattcaa gcggtccaac cagaacacct
     1021 gcatcaccca gaagccgatc gtccgggtcg gccagaaggt gcacaagggg caggtgctgg
     1081 gcgacggccc gtgcaccgag ctcggcgagc tggcgctggg acgcaatgtc ctggtcgcct
     1141 tcatgccgtg gcgcggctac aacttcgaag acgcgatcct cgtttccgaa cgcatggtga
     1201 aggacgacta ctacacgtcg attcacatcg aggagttcga gatcgagtcg cgcgacacga
     1261 agctcgggcc ggaagaaatc acgcgcgaca tcccgaacgt ctcggaaggg ttcctcaagg
     1321 acctcgacga gagcgggatc atccggatcg gcgcgaacgt gaagccgggc gacatcctcg
     1381 tgggcaaggt cacgccgaaa ggcgagacgc agctcacgcc ggaagagaag ctgctccgcg
     1441 cgatcttcgg cgagaaggcc ggcgacgtgc gcgacgcgag cctcatctgc ccgcccggca
     1501 tcgagggcat catcgtcggc gtgaagatct tctcccgcaa gggcatcgaa aaagatgacc
     1561 gggcgaaagc gatcgagcag gacgagctcg acatgatg
//
LOCUS       DASVII010000492         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_598052,
            whole genome shotgun sequence.
ACCESSION   DASVII010000492 DASVII010000000
VERSION     DASVII010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598052"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1545)
                     /gene="hrpA"
                     /locus_tag="VF309_06405"
     CDS             complement(<1..>1545)
                     /gene="hrpA"
                     /locus_tag="VF309_06405"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX7466252.1"
                     /translation="SKFFDGAPVIEVSGRTYPVEVRYRPEFFEVEDEDDEPVDMNEAI
                     AKAVDELARDRREGDILVFLPGEREIREAAEALRKHHPKNTEILPLFSRLSAEEQDRV
                     FERGPHRRIVLATNVAETSLTVPGVHFVIDTGLARVKRYSPRQKIDQLRIEPISQAAA
                     RQRAGRCGRVASGIAIRLYDEKEFLERPEFTTPEILRTSLASVILRMAALELGPIAEF
                     PFIEPPTPRQIEDGYRQLFELGAIDDARTLTPLGNELARLPVDPRIGRLLLASREFHC
                     LSEMLIVASALSIPDPRDRPQALREASDRAHEEFRDEASDFVQLMNLWKFFDHELVHK
                     KSGRKLYETCREHFLSYVRMREWRDLAGQLREMASELKIRDNPTPATYEQMHRALLTG
                     LIGNVGMKALDGDHYHGPRGLQFHIWPGSGLKKARPKWVMAGELQETTRVFARNAARI
                     EPDWIEKAAAHLVERTHVEPHWDKARGEVVAYENVSLFGLVLVARRKVSFGKFDPVKA
                     RTIFIEG"
BASE COUNT          245 a          483 c          549 g          268 t
ORIGIN      
        1 gccctcgatg aagatggtgc gcgccttcac gggatcaaat tttccgaaac taaccttgcg
       61 ccgcgcgacg agcaccaggc cgaagagcga gacgttctcg tatgcgacca cctcgccgcg
      121 cgccttgtcc cagtgcggct cgacgtgggt gcgctcgacg agatgggccg cggccttctc
      181 gatccagtcg ggctcgatgc gcgcggcgtt gcgcgcgaag acgcgagtcg tctcctggag
      241 ctcgcccgcc atcacccact tgggccgcgc cttcttcagc cccgatcccg gccagatgtg
      301 gaactgcagg ccgcgcggac cgtggtaatg atcgccgtcg agcgccttca ttccgacgtt
      361 gccgatgagg cccgtcagca acgcgcgatg catctgctcg taggtggcgg gcgtgggatt
      421 gtcgcggatc ttcaattcgg acgccatctc gcgcaattgt cccgcgaggt cgcgccactc
      481 acgcatgcgc acgtacgaaa ggaaatgctc gcggcacgtt tcgtagagct tgcggcccga
      541 cttcttgtgg acgagctcgt ggtcgaagaa cttccacagg ttcatgagct gcacgaaatc
      601 cgacgcttcg tcgcggaact cttcgtgcgc gcggtccgag gcttcgcgca acgcttgcgg
      661 gcggtcgcgg ggatcgggaa tcgaaagcgc cgaggcgacg atgagcatct cggaaaggca
      721 atggaattcc cgcgaggcga gaaggaggcg cccgatgcgc ggatcgacgg gaaggcgcgc
      781 gagctcgttg ccaagcggcg tgagagtgcg cgcgtcgtcg atggcgccca gctcgaagag
      841 ctggcggtag ccgtcttcga tttgccgcgg cgtgggcggc tcgatgaagg ggaattccgc
      901 gatgggtccc agctcgagcg ccgccatgcg caggatgacc gaagcgagcg aggtgcgcag
      961 gatctcgggc gtggtgaact cggggcgctc gaggaactcc ttctcgtcgt agaggcggat
     1021 cgcgatgccg ctcgcgacgc gcccgcagcg tcccgctcgc tggcgcgccg ccgcctgcga
     1081 gatgggctcg atgcgaagct ggtcgatctt ctgccgcggg gagtagcgct tcacgcgcgc
     1141 gaggcccgtg tcgatcacga agtggacgcc cggcacggtg agggaggttt ccgcgacgtt
     1201 ggtcgcgagg acgatgcgac ggtgggggcc gcgctcgaag acgcggtcct gctcctcggc
     1261 ggagaggcgc gagaagagcg ggaggatttc ggtgttcttc gggtggtgct tcctcagcgc
     1321 ttcggcggct tcgcggatct cgcgctcgcc cgggaggaag acgaggatgt cgccctcgcg
     1381 gcgatcgcgc gcgagctcgt cgaccgcttt cgcgatggcc tcgttcatgt ccacgggctc
     1441 gtcgtcctcg tcctcgactt cgaagaactc ggggcgatag cgcacctcga cgggataggt
     1501 gcgccccgag acctcgatca ccggcgcgcc gtcgaagaat ttcga
//
LOCUS       DASVII010000493         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_202150,
            whole genome shotgun sequence.
ACCESSION   DASVII010000493 DASVII010000000
VERSION     DASVII010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202150"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VF309_06410"
     CDS             <1..333
                     /locus_tag="VF309_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7466253.1"
                     /translation="YPDAFSRVREVDPDYAIPGGESRGQFHERVRSGFEALAAEHRGK
                     TVVVVTHGGVLATFYRHVNGLELRMPHRIPITNASYNVLAFDGERWSIETWNDTAHLE
                     GAEPFEEA"
     gene            complement(297..1706)
                     /gene="mltF"
                     /locus_tag="VF309_06415"
     CDS             complement(297..1706)
                     /gene="mltF"
                     /locus_tag="VF309_06415"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HEX7466254.1"
                     /translation="MIGRRILVFIACLAAGCEEAPKPVAPVSESGELVILTVNGPASY
                     FEDAQGLPSGFEYDLATLFAKELKARPNFVLIDNPARIDRVLRDGQAHLAAANLARHF
                     DFPGGLAWGPSYLSTQHQVVCRTGEEPRPKKLEDLAAKHVGVIEESVADYLLTDPTWT
                     KLPIERLAPGTSTADLLEQVARENLDCALVESTRYTLARRFFPQLEVAFNVGKPIDYA
                     WLVSTVDKKRILAAAGPFFERIKKDGTLKRLVDRYYGHATRFSAIDSASLLGQIATAL
                     PALKPHFEEAEVASGVDWRLLAAIGYQESHWDAKATSPTGVRGLMMLTEETADRLQIK
                     DRLDARASILGGARYFALLKDGLPPRLPEPDRTYLALAAYNIGIGHLEDARILAQKSG
                     LNPDKWQDVRQVISKLADPDSYKSLKHGYARGGEAMQLVDNIRNYYDILTRMEPRDTP
                     VVPLATDPQASSKGSAPSR"
     gene            1747..2445
                     /gene="ispD"
                     /locus_tag="VF309_06420"
     CDS             1747..2445
                     /gene="ispD"
                     /locus_tag="VF309_06420"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX7466255.1"
                     /translation="MPKVFALVLAAGQGTRIGDAVPKQYLPIAGKPMMFHSIEALAAV
                     SRIERLFVVLAPLDRHWGEHDWSALPEKIEAAFCGGSHRAESVLNTLKFLESRIARDD
                     WVLVHDAARPCIVTELVEQFLDELGDDQVGGLLAMPLADTLKRGGEQQRVEATIPRTG
                     LWRAQTPQMFRYELLRRGLERNPAATDEADAVESLGQAPRLVEGETSNMKVTFAEDIQ
                     IAELILEHRDRITL"
     gene            2448..2915
                     /gene="ispF"
                     /locus_tag="VF309_06425"
     CDS             2448..2915
                     /gene="ispF"
                     /locus_tag="VF309_06425"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7466256.1"
                     /translation="MRIGQGFDVHRLVAGRKLVIGGVEIAHDKGLLGHSDADVLLHAI
                     CDALLGAAALGDIGRHFPDSDARYRGIDSRELLRHVALLLRERGLRVVNLDATIIAEA
                     PRMAPHIPAMAAHIAADLAIALHCVNVKATTTEKLGFAGRGEGIAAQAICLIE"
     gene            2912..3337
                     /locus_tag="VF309_06430"
     CDS             2912..3337
                     /locus_tag="VF309_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466257.1"
                     /translation="MSPRRALIIVALAALVATGIEPRLLQMPFIDRDANARALALFPD
                     RQMPQYPRFLAAVRERTQPGDKIALLVPTMEWYGGYSQAYFRASYFLTGREVLPLVDP
                     QNAPTDNFAAARYVAAFGVTLATPADVVWQGDGGTLYRR"
     gene            3334..>3629
                     /locus_tag="VF309_06435"
     CDS             3334..>3629
                     /locus_tag="VF309_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466258.1"
                     /translation="MTATLAAILGLTLLGVPITLAVDRRAHGALLAGLSFLYGSGAIF
                     LVLLALSAAGVRWTLAVVAAAALAVFAIAAFVAMRQPATGKRHAGARPHVIDLLT"
BASE COUNT          553 a         1244 c         1248 g          584 t
ORIGIN      
        1 tatcccgatg cgttctcgcg ggtgcgggaa gtcgatcccg actacgcgat tcccggcggc
       61 gaaagccgcg gccagttcca cgagcgcgtg cgctcgggat tcgaggcgct cgccgccgag
      121 catcgcggca agacggtcgt cgtggtgacc cacggcggcg tgctcgccac gttctaccgc
      181 cacgtgaacg gcctcgagct acgaatgccg catcgcatcc ccatcaccaa cgcgtcgtac
      241 aacgtgctgg cgttcgacgg ggagcgctgg tccatcgaga cgtggaacga taccgctcac
      301 ctggaagggg ccgagccctt cgaggaggcc tgagggtcgg tggcgagcgg caccaccggg
      361 gtatcccgcg gctccatgcg cgtcaggatg tcgtagtagt tgcggatgtt gtcgacgagc
      421 tgcatcgcct cgcccccgcg cgcatagccg tgcttgagcg acttgtacga atccggatcg
      481 gcgagcttcg aaatcacctg gcgcacgtcc tgccacttgt cgggattgag ccccgacttc
      541 tgcgcgagga tgcgcgcgtc ctcgaggtgg ccgatgccga tgttgtaggc ggcgagcgcg
      601 aggtaggtgc gatcgggctc gggaaggcgc ggcggcagcc cgtccttgag gagcgcgaag
      661 tagcgcgctc ctccgaggat gctcgcgcgc gcatccagtc gatccttgat ctgcaggcga
      721 tcggcggtct cctcggtgag catcatgagc ccgcgcacgc ccgtgggaga cgtggccttc
      781 gcatcccagt gcgattcctg gtagccgatc gccgcgagga ggcgccaatc gacgccgctc
      841 gcgacctccg cctcctcgaa gtgcggcttc agggccggca gcgcggtcgc gatctgcccg
      901 agaagcgacg cggagtcgat cgcgctgaag cgcgtggcgt ggccgtagta gcgatccacg
      961 aggcgcttga gcgtgccgtc cttcttgatg cgctcgaaga aggggcccgc cgcggcgagg
     1021 atcctcttct tgtccaccgt ggagacgagc cacgcgtagt cgatgggctt gccgacgttg
     1081 aaggcgacct cgagctgcgg gaagaagcgc cgcgcgagcg tgtaacgggt cgattccacc
     1141 agcgcgcagt cgaggttttc cctcgccacc tgctcgagga gatcggcggt cgaagtcccc
     1201 ggtgcgagcc tctcgatcgg aagcttcgtc cacgtgggat ccgtgaggag gtagtcggcg
     1261 acggactcct cgatcacgcc gacgtgcttg gcggcgaggt cttcgagctt cttcggcctc
     1321 ggctcctcgc ccgtgcggca gaccacctgg tgctgcgtgc tgagatacga cggtccccac
     1381 gcgagcccgc cggggaaatc gaaatgccgc gcgaggttcg cggccgcgag gtgcgcttgg
     1441 ccgtcgcgca gcacgcgatc gatgcgcgcg ggattgtcga tgagcacgaa gttgggccgg
     1501 gccttgagct ccttcgcgaa gagcgtggcg aggtcgtact cgaatcccga cggcaggccc
     1561 tgcgcgtcct cgaagtagct cgcgggcccg ttcaccgtga gaatcaccag ctcgcccgat
     1621 tcggaaaccg gggcgacagg tttcggggcc tcttcgcaac cggcggcgag acaagcgatg
     1681 aacacgagga ttcgccgccc gatcatgggc cgacagtata atggaggccg ttcgactcat
     1741 tccctcatgc caaaagtctt cgcattggtg ctcgccgccg gacagggcac acgcatcggc
     1801 gatgccgttc cgaagcaata cctccccatc gccggcaagc cgatgatgtt ccattcgatc
     1861 gaggcgctgg ccgcggtgtc gcgcatcgag cggctcttcg tcgtgctcgc gcccctcgac
     1921 cgccactggg gcgagcacga ctggagcgcg ctgcccgaga agatcgaggc agccttctgc
     1981 ggcggctccc atcgcgcgga gagcgtcctc aacacgctga agttcctcga atcgcgcatc
     2041 gccagggacg actgggtgct ggtccacgac gcggcgcgcc cgtgcatcgt gaccgagctc
     2101 gtcgagcagt tcctggacga gctcggcgac gatcaggtgg gcgggctcct cgcgatgcct
     2161 ctcgcggaca cgctcaagcg cggcggggag cagcagcgcg tcgaagccac gatcccgcgc
     2221 acgggcctgt ggcgcgcgca gaccccgcag atgttccgct acgagctgct gcgccgcggc
     2281 ctcgagagga atcccgcggc cacggacgag gccgacgcgg tggaatccct cggccaggcc
     2341 ccgcggctcg tcgaaggaga gacgagcaac atgaaggtca cgttcgccga ggacatccag
     2401 atcgcggagc tcatcctcga gcatcgcgac cggatcacgc tctgagcatg cgcatcggcc
     2461 agggattcga cgttcatcgg ctggtcgcgg ggcgcaagct cgtgatcggc ggcgtggaga
     2521 tcgcccacga caagggcctc ctcggtcaca gcgacgccga cgtgctgctg cacgcgatct
     2581 gcgacgcgtt gctcggcgcc gcggcgctgg gagacatcgg caggcacttt cccgacagcg
     2641 acgcgaggta ccggggcatc gacagccgcg agctcctgcg ccatgtcgcg cttctcctgc
     2701 gggagcgggg gctgcgcgtc gtgaacctcg acgcgacgat catcgccgag gcgccgcgca
     2761 tggcgccgca cattcccgcc atggccgcgc acatcgcggc cgatctcgcg atcgcgctgc
     2821 attgcgtcaa cgtgaaggcg acgacgacgg agaagctcgg attcgcgggg cggggcgagg
     2881 gcatcgcggc gcaggcgatc tgcctcatcg agtgagcccc cgccgcgcgc tcatcatcgt
     2941 ggccctggcg gccctcgtgg cgacgggcat cgagccacgc ctgctgcaga tgccgttcat
     3001 cgaccgcgac gccaacgcgc gcgcgctcgc gctctttccc gatcggcaaa tgccgcagta
     3061 tccgcgcttc ctcgccgccg tccgggaacg cacccagccc ggcgacaaga tcgcgctcct
     3121 cgtgcccacc atggagtggt acgggggtta ctcccaggcg tacttccgcg cgagctactt
     3181 cctgacgggg cgcgaggtgc tgccgctcgt cgatccgcag aatgcgccga cggacaactt
     3241 cgccgccgcg cgctacgtcg ccgcgttcgg cgtgacgctc gccacgccgg ccgacgtcgt
     3301 ctggcaaggc gacgggggaa cgctgtaccg gcgatgacag ccacgctcgc cgcgatcctc
     3361 ggcctcacgc tcctcggcgt ccccatcacg ctcgccgtcg atcgccgcgc ccatggcgcg
     3421 ctcttggcgg gactgtcgtt tctctatggc agcggcgcga tctttctcgt gctgctggcg
     3481 ctctcggccg cgggcgtccg ctggacgctt gcggtcgtcg cggccgcggc gctcgcggtg
     3541 ttcgcgatcg ccgcgttcgt ggcgatgcgg caaccggcga ccggcaagcg gcacgctggc
     3601 gcgcgcccgc atgtcatcga cctcctcac
//
LOCUS       DASVII010000494         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_382768,
            whole genome shotgun sequence.
ACCESSION   DASVII010000494 DASVII010000000
VERSION     DASVII010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382768"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="VF309_06440"
     CDS             complement(<1..1860)
                     /locus_tag="VF309_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7466259.1"
                     /translation="MKSVSSFLYRTPWWVLLGGGFVLLLALGIFTTPFHTIQLDKAGA
                     TAAENRAIKSEINSAFSESAIDLARGVVKEMLEHTKDPERVQELRDALVDLDTARQSV
                     REAGAEVLRAKRQAAEDVTGAIKEAANAIKEARKEAARALKDARVEHREKIEKSLDES
                     LDAATQAGAEARDAGGAAIEKVDPKKRVIVVGPNPDKPLLDIEVGPGDANVAPLPAEL
                     KREIRKKVTGDLYRIGVGAGLILIFIPLFVLAVVSKFFIDRSRAAQNMAELKRKEAEY
                     HRMSQQVTEAKLSALQAQVEPHFLYNTLASVQALTEVDPQQANAMTGHLIAYLRNALP
                     KMRESVSTVGQEIELVRAYLNILQMRMGKRLAFEIDVPPELMEAPFPPLMLPSLVENA
                     IKHGLEPQREGGKVTIRATEADGTLRMSVLDTGRGFAETLGAGVGLANIRERLAALYG
                     DKGKLTLESREPHGVEAAIEVPREGTRAATAGAIPMAPEAAPPPAAPQSAAAKALAAM
                     GSAERAWRKGLSFAFVVLVVIAAVVAGLAMVAVATGLIDVQNGQETIGGPAGALIGTA
                     GIAAGFAVVVLALAIVLAVLYGLGFLFVGLAIFIPLFVITIVSKFFIDRSRAAQ"
     gene            complement(2146..>2716)
                     /locus_tag="VF309_06445"
     CDS             complement(2146..>2716)
                     /locus_tag="VF309_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7466260.1"
                     /translation="VNGLEAARLLAGRAHVVFVTAFDQYAVEAFERGAIDYVLKPFNE
                     ERLAITVARLKERLDSKPAMLEEIVEGLAARLAPRGSEHLRWIKASVGSNVRLIPVEE
                     VLFFRSDEKYTRVVTAEAEALIRKPIRELLDELDPERFWQIHRATIVNVDHIASVRRG
                     LKDQAEIALRDHGETLVVSRAFTHLFKQM"
BASE COUNT          413 a          923 c          945 g          435 t
ORIGIN      
        1 ctgcgcggcg cgcgagcggt cgatgaagaa cttggagacg atcgtgatca cgaagagcgg
       61 gatgaagatc gcgaggccga cgaagaggaa cccgagcccg tagagcacgg cgagcacgat
      121 ggcgagcgcg agcacgacga ccgcgaaccc cgccgcgatc cccgcggtgc cgatgagcgc
      181 gcccgcgggg ccgccgatcg tttcctgccc gttctgcacg tcgatgaggc ccgtggccac
      241 ggcgaccatg gcgagccccg cgacgaccgc ggcgatcacc acgagcacga cgaaggcgaa
      301 cgaaagcccc ttgcgccacg cgcgctccgc ggagcccatg gcggcgagcg ccttggccgc
      361 ggcgctctgc ggcgccgccg gcggcggggc cgcctcgggc gccatgggaa tcgcgccggc
      421 ggtggcggcc cgcgtgcctt cgcgcggaac ctcgatcgcc gcctcgaccc cgtggggctc
      481 gcgtgactcc agcgtgagct tgcccttgtc gccatagagc gccgcgagcc tttcgcgaat
      541 gttcgcgagg ccgactcccg cgccgagcgt ctcggcgaag ccgcgcccgg tgtcgagcac
      601 actcatgcgc agcgtgccgt cggcctcggt cgcgcgaatg gtgaccttgc cgccctcgcg
      661 ctgcggctcg aggccgtgct tgatggcgtt ctcgacgagc gagggcagca tgagcggcgg
      721 gaacggcgcc tccatgagct ccggcggcac gtcgatctcg aaggcgaggc gcttgcccat
      781 gcgcatctgc aggatgttga ggtaggcgcg caccagctcg atctcctggc cgacggtcga
      841 gaccgactcg cgcatcttcg gcaacgcgtt cctcaggtac gcgatcaaat gacccgtcat
      901 cgcgttggcc tgttgcgggt cgacctccgt cagcgcctgg acgctcgcga gcgtgttgta
      961 gaggaagtgc ggctccacct gcgcctgcaa cgccgacagc ttggcttcgg tcacctgctg
     1021 gctcatgcgg tggtattcgg cctccttcct cttcagctcc gccatgttct gcgcggcgcg
     1081 cgagcggtcg atgaagaact tggagaccac cgcgagcacg aagagcggga tgaatatgag
     1141 gattaggccc gcgcccacgc cgatgcggta gagatcgccc gtgactttct tgcggatctc
     1201 gcgcttcagc tccgccggaa ggggcgccac gttggcatcg cccgggccga cctcgatgtc
     1261 gagcagcggc ttgtcggggt tcggccccac gacgatcacg cgcttcttcg ggtcgacctt
     1321 ctcgatggcg gcgcccccgg cgtcgcgggc ctcggccccc gcctgcgtcg cggcgtcgag
     1381 cgattcgtcg agggacttct cgatcttctc gcgatgctcc acgcgggcgt ccttcaacgc
     1441 gcgtgccgcc tccttcctcg cctccttgat cgcgttggcc gcttccttga tggctccggt
     1501 cacgtcctcg gcggcctggc gcttggcgcg aagcacctcg gcccccgcct cgcgcacgct
     1561 ctggcgcgcc gtgtcgaggt cgacgagcgc gtcgcgaagc tcctgcacgc gctcggggtc
     1621 cttggtgtgc tcgagcatct ccttcaccac gccgcgcgcg aggtcgatgg cgctctcgga
     1681 gaacgcggaa ttgatctcgc tcttgatcgc gcggttctcg gcggcggtgg cgccggcctt
     1741 gtcgagctgg atggtatgga acggcgtcgt gaagatgccg agcgccagca ggaggacgaa
     1801 ccctccgccg agcagcaccc accacggcgt gcggtacaag aagctcgaga cagatttcat
     1861 cgttttcccg ggtcgcgccc tagcgcgaca ttattcagta atccgcgagc aatgcgatga
     1921 ccgcggtcga aagcgaaaac agcagcacgc cgaagaacac cagcgccatg ccgagggaca
     1981 ggtcgagcga agggatgatg gacatgggcc gggcctctcc tgtgagccgt tcacgcggct
     2041 ttgtgggggt actttagggg cgccgccccc ggcattccac ggccgggcgg cgaactgcag
     2101 aaaacggggg gtgaaatgcc cccggcgggg ggcgaagcgc cgccgctaca tctgcttgaa
     2161 gaggtgggtg aaggcccggg agaccaccag ggtctcccca tggtcgcgaa gggcgatctc
     2221 ggcctggtcc ttgaggccgc gcctgaccga ggcgatgtga tcgacgttga cgatggtggc
     2281 gcgatggatc tgccagaagc gctccgggtc gagctcgtcg aggagctcgc ggatgggctt
     2341 gcggatgagc gcctcggctt ccgccgtaac cacgcgcgtg tatttctcgt cggaccggaa
     2401 gaagagcact tcctcgaccg gaatgaggcg cacgttggag cccaccgagg ccttgatcca
     2461 gcgcaggtgc tcggagcccc gcggcgcgag ccgcgcggcg agcccctcga cgatctcctc
     2521 gagcatcgcc ggcttggaat cgaggcgctc cttcagccgc gccacggtga tcgcgaggcg
     2581 ctcctcgttg aagggcttca ggacgtagtc gatggcgccg cgctcgaagg cctcgacggc
     2641 gtactggtcg aacgcggtca cgaacacgac gtgggcccgg cccgcgagca gccgcgccgc
     2701 ctccaggccg ttcacg
//
LOCUS       DASVII010000495         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_94346, whole
            genome shotgun sequence.
ACCESSION   DASVII010000495 DASVII010000000
VERSION     DASVII010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94346"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VF309_06450"
     CDS             complement(<1..1576)
                     /locus_tag="VF309_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7466261.1"
                     /translation="MREKFAELSTRIATATAQPQAPEAVAEGYVRIAVANMANAIKFI
                     SVQRGHDVTEYTLACFGGAGGQHACLVADELGMKRVYIHPLAGVLSAYGMGLAQVRAL
                     REEAVERRLEDGALTVIEEKCVTLGEQCVHELAEQGVAFEKLEKRVHLKYEGTDTSLA
                     VPLASIERMRAEFERLYRSRFSFLMPDRALVAEAVSVEAVGRSDAGPGLIVASPVSIA
                     KPAPHARVKMTTRGAEHDTPVYLRDRLAPGTRIAGPAIIAERNATTVVEPEWEARVTE
                     LYHLVLERVVERKARYALGTTADPVMLEVFNNLFMSIAEQMGVRLAQTAYSVNIKERL
                     DFSCALFDADGNLIANAPHMPVHLGSMGESIKTVIAKNAGAMKPGDVYVLNDPYNGGT
                     HLPDVTVITPVYLDEAKPLFYVGSRGHHADIGGITPGSMPPFSKTIEEEGVLLDNVKL
                     VEGGRMLEKEMRELLASARYPARNPDQNIADLRAQVAANEKGVQELKRMVAHFGLDVV
                     KAYMGHVQDNAEECVRR"
BASE COUNT          229 a          530 c          550 g          271 t
ORIGIN      
        1 cgcggcgtac gcattcctcg gcgttgtcct ggacgtggcc catgtacgcc ttcaccacgt
       61 cgaggccgaa gtgcgcgacc atgcgcttca gctcctggac gcccttctcg ttcgccgcga
      121 cctgcgcgcg caggtccgcg atgttctggt cgggattgcg cgccggatag cgcgccgagg
      181 cgaggagctc gcgcatctcc ttctcgagca tgcggccgcc ctcgacgagc ttcacgttgt
      241 cgaggaggac gccttcctcc tcgatggtct tcgagaaggg cggcatggaa ccgggggtga
      301 tgccgccgat gtcggcgtgg tggccccggc tgccgacgta aaacaaaggc ttcgcctcgt
      361 caagatagac aggcgttatc acggtgacgt cggggagatg ggtgccgccg ttgtacgggt
      421 cattcaagac atagacgtcg cccggcttca tggcgccggc gttcttggcg atcacggtct
      481 tgatgctctc gcccatggag cccaggtgca ccggcatgtg gggggcgttg gcgatcaggt
      541 tgccgtcggc gtcgaagagg gcgcacgaga agtcgaggcg ctccttgatg ttcaccgagt
      601 aggcggtctg ggcgaggcgc acgcccatct gctccgcgat cgacatgaag aggttgttga
      661 agacttcaag catcaccggg tccgcggtgg tgccgagcgc atagcgcgcc ttgcgctcga
      721 cgacgcgctc gagcacgagg tgatagagct cggtcacgcg cgcctcccac tcgggctcga
      781 ccaccgtcgt cgcattgcgc tcggcgatga tcgcggggcc cgcgatgcgc gtccccgggg
      841 cgagcctgtc gcgcaggtag accggcgtgt cgtgctccgc gccgcgggtc gtcatcttca
      901 cgcgcgcgtg gggcgcgggc ttggcgatcg acaccgggct cgcgacgatg aggcccgggc
      961 ccgcgtcgga gcggccgacc gcctcgaccg acaccgcctc ggcgacgagc gcgcgatcgg
     1021 gcatgaggaa gctgaagcgg cttcgataga ggcgctcgaa ttccgcgcgc atccgctcga
     1081 tcgaggcgag cggcacggcg agcgaggtgt cggtgccttc gtacttgagg tgcacgcgct
     1141 tctcgagctt ctcgaacgcg acgccctgct cggcgagctc gtgcacgcac tgctctccca
     1201 gcgtcacgca cttctcctcg atgacggtga gcgcgccatc ctcgaggcgc cgctcgaccg
     1261 cctcctcgcg cagcgcgcgc acctgcgcga ggcccatgcc gtacgccgac agcactccgg
     1321 ccaggggatg gatatacacg cgcttcatgc cgagctcgtc ggcgacgagg cacgcgtgct
     1381 gccctccggc gccgccgaag cacgcgagcg tgtattcggt cacgtcgtgg ccgcgctgca
     1441 ccgagataaa cttgatcgcg ttggccatgt tcgcgaccgc gatgcggaca tatccctcgg
     1501 cgacggcttc cggcgcctgg ggctgcgcgg tggccgtggc gatccgcgtc gagagctcgg
     1561 cgaacttctc gcgcacgacc
//
LOCUS       DASVII010000496         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_94357, whole
            genome shotgun sequence.
ACCESSION   DASVII010000496 DASVII010000000
VERSION     DASVII010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94357"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /gene="yajC"
                     /locus_tag="VF309_06455"
     CDS             <1..232
                     /gene="yajC"
                     /locus_tag="VF309_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7466262.1"
                     /translation="MYFIMIRPQMKKAKEHKTMLEALQKGDEVVAVGILGKVARINDT
                     YLSLEIAPNVTIQVQRGAVTQLLPKGTVKDAQ"
     gene            236..2122
                     /gene="secD"
                     /locus_tag="VF309_06460"
     CDS             236..2122
                     /gene="secD"
                     /locus_tag="VF309_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7466263.1"
                     /translation="MNRYPAWKYILIAVAILVSVVYGAPNLFGEVPAVQVSPQRATVK
                     IDNELRASLEEALKAAKFAPVSVEQSDATLRFLFADTDTQAKAIDVIKAKAGAGFVVA
                     LNLVPNSPHWLQALGSKPMYLGLDLRGGVHFLLQVDMKAAADKSVNRYLGDVRGLLRE
                     KKIYYSALAREGDRIVARFKEPEQRAAALKAMSDAMPDLTIREQDSGGDSALVASIKP
                     EVVKREQDLALQQNIQTLRNRVNELGVAEPIVQQQGADRIVVQLAGVQDPTRAKEILG
                     RTATLEVRLVAEESAAGPGQDAFLQYRDQPAPFGTDKFLDLDGAPVLVKKAIVVTGDR
                     IKDAQPGFDQQHSGAIVSLQLDATGGRIMRQTTRENVKKRMAMMLVERNSTVVLTWPV
                     IQEEFGARFQISGMPDATEAKNLALLLRAGALAAPMDIIEERTIGPSLGKENIEKGFH
                     AVLWGFAAIAVFMIFYYSVFGLISVAALACNLLFLLGILSMMQATLTLPGIAAVALTL
                     GMAIDSNVLINERVREELRSGMTPQMAIQAGYERAIGTILDSNVTTLIAGLALLTFGS
                     GPVRGFAVVHCLGILTSIFSAVVVSRGIVNITYGGRKKVQKLSIGNTDWHRKDGETPK
                     KVAV"
     gene            2125..>2374
                     /locus_tag="VF309_06465"
     CDS             2125..>2374
                     /locus_tag="VF309_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7466264.1"
                     /translation="MEFFRIRRSIPFMRHALVFNVISLVVFVLAIFFLATKGLHFSIE
                     FTGGTVIEVSYPQAADADGVRATLDKIGLKDVSVQTFGS"
BASE COUNT          419 a          783 c          792 g          380 t
ORIGIN      
        1 catgtacttc atcatgatcc ggccgcagat gaagaaggcg aaggagcaca agacgatgct
       61 cgaggcgctg cagaagggcg acgaggtggt cgccgtcggc atcctcggaa aggtcgcgag
      121 gatcaacgac acctacctga gcctcgagat cgcccccaac gtgaccatcc aggtgcaacg
      181 cggcgcggtc acgcaacttc tgcccaaggg caccgtcaag gacgcgcagt agggcatgaa
      241 caggtatccc gcctggaagt acatcctcat cgcggtcgcg atcctcgtga gcgtggtcta
      301 cggcgcgccc aacctcttcg gcgaggtgcc cgcggtgcag gtctcgccgc aacgcgccac
      361 cgtcaagatc gacaacgagc tgcgcgcgag cctcgaggaa gcgctcaagg ccgcgaagtt
      421 cgcccccgtc tccgtcgagc agtccgacgc gaccctgcgc tttctcttcg cggacacgga
      481 cacgcaggcg aaggcgatcg acgtcatcaa ggcgaaggcg ggcgcgggct tcgtggtcgc
      541 gctgaacctc gtgccgaatt cccctcactg gctgcaggcg ctcggctcga agccgatgta
      601 cctgggcctc gacctgcgcg gcggcgtgca tttcctgctg caggtcgaca tgaaggccgc
      661 ggccgacaag tccgtcaacc gctacctcgg cgacgtgcgc gggctcctgc gcgagaagaa
      721 gatctactac tcggcgctcg ctcgcgaggg cgacaggatc gtcgcgcgct tcaaggagcc
      781 cgagcagcgg gcggcggcgc tgaaggcgat gagcgatgcg atgcccgatc tcaccatccg
      841 cgagcaggat tcgggcgggg actcggcgtt ggtcgcgagc atcaagccgg aggtggtgaa
      901 gcgcgagcag gatctcgcgc tccagcagaa catccagacg ctgcgcaacc gcgtgaacga
      961 gctgggcgtg gccgagccga tcgtgcagca gcagggcgcc gatcgcatcg tggtgcagct
     1021 ggccggcgtg caggatccga cgcgcgcgaa ggagatcctc gggcgcacgg cgacgctcga
     1081 agtgcgcctc gtcgccgagg agagcgccgc cggtccgggg caggacgctt tcctgcagta
     1141 tcgcgaccag ccggcgccct tcggcaccga caagttcctg gacctcgacg gcgcgcccgt
     1201 gctcgtgaag aaggcgatcg tcgtgacggg cgaccgcatc aaggacgcgc agcccggctt
     1261 cgaccagcag cacagcggcg ccatcgtttc gctgcagctc gacgcgacgg gagggcgcat
     1321 catgcgccag accacgcgcg agaacgtgaa gaagcgcatg gcgatgatgc tggtcgagcg
     1381 caacagcacg gtggtcctca cgtggcccgt gatccaggag gaattcggcg cgcgcttcca
     1441 gatttccgga atgcccgatg ccacggaggc gaagaacctc gcgctgctgc tgcgcgccgg
     1501 ggcgctggcc gcgcccatgg acatcatcga ggagcgcacc atcgggccga gcctcggcaa
     1561 ggagaacatc gagaagggct tccacgccgt gctgtggggc ttcgccgcca tcgccgtctt
     1621 catgatcttc tactactcgg tgttcggcct gatctcggtc gccgcgctcg cgtgcaacct
     1681 gctcttcctg ctgggcatcc tttccatgat gcaggcgaca ctcacgttgc cgggcatcgc
     1741 ggcggtcgcc ctcacgctcg gcatggcgat cgactccaac gtgctcatca acgagcgcgt
     1801 gagggaggag ctgcgctccg gcatgacgcc gcagatggcc atccaggccg ggtacgagcg
     1861 ggcgatcggg acgatcctgg attcgaacgt gaccacgctc atcgccggcc tcgcgctcct
     1921 cacgttcggc tccggcccgg tgcgcggctt cgcggtggtc cactgcctgg gcatcctcac
     1981 ctcgatattc agcgcggtgg tggtctcgcg cggcatcgtg aacatcacct acggcggccg
     2041 caagaaggtg cagaagctct cgatcggaaa caccgattgg caccgcaagg acggcgagac
     2101 tcccaagaaa gtggcggtgt aaccatggaa ttcttccgca tccgccgttc catcccgttc
     2161 atgcgccacg cgctggtgtt caacgtcatc tcgctcgtgg tgttcgtgct ggcgatcttc
     2221 ttcctcgcga ccaaggggtt gcacttctcg atcgagttca ccggcgggac ggtgatcgag
     2281 gtgagctatc cgcaggcggc cgatgccgac ggggtccgcg cgacgctcga caagatcggc
     2341 ctgaaggacg tctcggtgca gaccttcggc tcct
//
LOCUS       DASVII010000497         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_94378, whole
            genome shotgun sequence.
ACCESSION   DASVII010000497 DASVII010000000
VERSION     DASVII010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94378"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VF309_06470"
     CDS             complement(<1..598)
                     /locus_tag="VF309_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466265.1"
                     /translation="MKRILAALLVLSSPCAFAQQVPIIEYESVPNPVKLPKDMHLGEV
                     TGVAVNSKGNIYVFSRGNTTGPAYAAAAAQLLEFAPDGHFIREIGKNLYAWSFAHSVR
                     IDKDDNIWAVDKGSDMVVKFNKDGQVAMVFGRKQEASDEETGPVKHPKPPLPAEDGRF
                     RQVTDVTWDPAGNIYISDGYINSRVAKADKNGNWLKSWG"
     gene            complement(684..1586)
                     /locus_tag="VF309_06475"
     CDS             complement(684..1586)
                     /locus_tag="VF309_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7466266.1"
                     /translation="MKIDTLGVQAFVAIADHGSFSRAAQALHITQTGLTRRLQNLEAF
                     LGVKLVERTTRSVALTGIGRDFLPQGRRLLADLTTALAEIRETGKALRGDLTIACVPT
                     VGIHYLPRIVQQYSSMHPENRIRILDHPSHDVVRAVRGREAEFGINIQGAHDAELVSV
                     PLLQDRFVLVCRDDHPLAGRKRIAWRQLEAHPLILAGHESGNRHLLDAALAGEALRLQ
                     SFYEVQHSSTAVGMVAEGVAAAVVPALALQDGAYPRLRTVALAHPVVSRTIVLLSRRK
                     AYLAPAAQALYDLVRATAAKLAPR"
     gene            1672..1995
                     /locus_tag="VF309_06480"
     CDS             1672..1995
                     /locus_tag="VF309_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466267.1"
                     /translation="MAESIMEAWTALMGELEARKRELGEAVRAYPTPIARCDDQLPKA
                     IAQRDAAAMLVRSAAEVEGSRGELPMGQWRERVRDFAQRLGAADDDGAFEASLGRLLA
                     ALEAR"
     gene            1992..2738
                     /locus_tag="VF309_06485"
     CDS             1992..2738
                     /locus_tag="VF309_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX7466268.1"
                     /translation="MTSVLVYAALGIASLVMSFISGILGMAGGMILMGVLLAFLPLAA
                     AMMLHGICQFAANGWRAFMLRREIDWRVFRGYAIGAILALGLFALLRLVVSKPVALIA
                     MGLTPFVALALPEKLHLNVERRGQSFACGFICSALALTAGISGPILDVFFVRSKMTRH
                     KVVATKASTQSLSHVLKIIYFGGVLAVEGGAVEPALAAMMVALAFIGTSLSRQVLERI
                     TDASFRLWTRWTVMTLGLIYLASGVVSLAR"
     gene            complement(2863..>3277)
                     /locus_tag="VF309_06490"
     CDS             complement(2863..>3277)
                     /locus_tag="VF309_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466269.1"
                     /translation="GDQWYSSPWFNGPWHLVAPELVPLYVLRVPVRYYRHPPAFFGGW
                     RADLSPRWGDHWGREWEQRRTGWDHWDHRSAPRAAPLPTYQRQYSGNRYPQEQRQQEL
                     HVQNYRYQPRDAETRQHYEQHYQSRGGDRHEGDRR"
BASE COUNT          473 a         1057 c         1179 g          568 t
ORIGIN      
        1 cgccccagga cttcagccag ttgccgttct tgtcggcctt ggcgacgcgc gaattgatat
       61 agccgtcgct gatgtagatg ttgcctgcgg gatcccaggt gacgtcggtc acctggcgga
      121 agcgcccgtc ctccgcgggc agcggcggct tgggatgctt cacgggaccg gtctcctcgt
      181 cggaggcttc ctgcttgcgc ccgaacacca tggcgacctg gccgtccttg ttgaacttca
      241 cgaccatgtc ggagcccttg tccacggccc agatgttgtc gtccttgtcg atgcgcaccg
      301 agtgcgcgaa cgaccacgca tagaggttct tgccgatctc gcggatgaaa tggccgtcgg
      361 gagcgaattc cagcagctgc gcggcggcgg cggcataggc cggtcccgtc gtgttgcccc
      421 gggagaagac gtagatgttg cccttcgagt tcaccgcgac gccggtgacc tcgccgaggt
      481 gcatgtcctt cggaagcttg accggattgg gcaccgactc gtactcgatg atgggcacct
      541 gctgcgcgaa cgcgcacggg ctcgacagca ccagcaatgc agcgagaatc ctcttcatga
      601 cgtcctcctc cgttgtacct gcgctcgatt cggcgccgac gccttcatcg gcggcgggct
      661 gcaagtgtat caacgggcgg ggatcagcgt ggcgcgagct tcgcggcggt cgcccggacg
      721 aggtcgtaaa gcgcctgggc ggcgggcgcg agataggcct tgcgccgcga caggagcacg
      781 atggttcgcg acaccacggg atgggcgagc gcgaccgttc gcagccgcgg atacgcgccg
      841 tcttgcaggg cgagcgccgg caccaccgcg gcggcgactc cctcggccac catgcccacg
      901 gcggtcgagc tgtgctggac ttcgtagaag gactggagcc gcagggcctc gcccgccagg
      961 gcggcgtcga ggagatggcg attgccgctc tcgtgccccg cgaggatgag cggatgcgcc
     1021 tcgagctgcc tccacgcgat gcgcttgcgg cccgcgagcg gatggtcgtc gcggcacacc
     1081 agcacgaagc gatcctgcag cagcggaacg ctcacgagct cggcgtcgtg ggccccctgg
     1141 atgttgatgc cgaattccgc ctcgcggccc cgcaccgcgc gcaccacgtc gtgggaggga
     1201 tggtcgagga tgcgaatgcg attctccggg tgcatcgacg agtactgctg gacgatgcgc
     1261 ggcaggtagt ggatgccgac cgtgggcacg caggcgatcg tcaggtcgcc gcgcagcgcc
     1321 ttgccggtct cgcggatttc cgcgagcgcg gtcgtgaggt cggcgaggag cctgcgcccc
     1381 tgcggcagga aatcgcgccc gatcccggtg agcgccaccg agcgcgtggt gcgctccacc
     1441 agcttcacgc cgaggaaggc ctcgaggttc tgcaggcggc gcgtgaggcc cgtctgcgtg
     1501 atgtgcaggg cctgcgcggc gcggctgaag ctgccgtggt cggcgatggc gacgaaggcc
     1561 tgcacgccca gggtgtctat cttcatgcga aatcctcatg aatcgcgaga ataatgtcat
     1621 tttaccgcgg gatcgattgc gccgagaatg cgcctttgcg gagggcgggg tatggctgag
     1681 tcgatcatgg aggcgtggac ggcgttgatg ggcgagctcg aggcccgcaa gcgcgagctg
     1741 ggcgaggccg tgcgcgccta tccgacgccc atcgcgcgtt gcgacgacca gcttccgaag
     1801 gcgatcgccc agcgcgacgc ggcggcaatg ctcgtgagat cggcggccga ggtcgagggt
     1861 tcgcgcggcg agctgccgat ggggcagtgg cgtgagcggg tgcgcgactt cgcgcagcgt
     1921 ctcggcgcgg ccgacgacga tggggctttc gaagcgagcc ttgggcgcct cctcgccgca
     1981 ctggaggcgc ggtgacgtcg gtgctggtgt acgcggcgct cggaatcgcc tcgctcgtca
     2041 tgtccttcat ctcgggcatc ctcggcatgg cgggcgggat gatcctcatg ggcgtgctgc
     2101 tcgcgttcct gccgctcgcg gccgcgatga tgctgcacgg catctgccag ttcgccgcca
     2161 acggctggcg cgccttcatg ctgcgccgcg agatcgactg gcgggtcttc cgcggctacg
     2221 cgatcggcgc gatcctcgcc ctcgggctct tcgccctgct ccggctcgtg gtgagcaagc
     2281 ccgtcgcgct gatcgcgatg gggctcacgc ccttcgtggc gctggcgctg cccgagaagc
     2341 tgcacctcaa cgtcgagcgc cgcggccagt cgttcgcgtg cggcttcatc tgctcggcgc
     2401 tcgcgttgac cgcgggcatc tcgggtccga tcctcgacgt cttcttcgtg cgctcgaaga
     2461 tgacgcggca caaggtcgta gccaccaagg cctcgacgca aagcctgagc cacgtgctca
     2521 agatcatcta tttcggcggc gtgctcgcgg tcgagggcgg cgccgtcgag cccgcgctcg
     2581 cggcgatgat ggtggccctc gcgttcatcg ggacgtcgct gtcgcgccag gtcctcgagc
     2641 gcatcacgga cgcatcgttc cgcctctgga cgcgctggac ggtgatgacg ctggggttga
     2701 tctatctcgc gagcggcgtg gtgagcctcg cgcgatagcc gccaagcgtg ttctccgtgt
     2761 gatcgtcttg agagcgccag cgcagggagt tggattcaaa cgacgcgaga aggaaaaagc
     2821 cccgcatttg cggggctttc ttgacgaacg tgaagcgcaa gatcagcggc ggtcgccttc
     2881 gtggcggtcg cccccgcggg actggtagtg ctgctcgtag tgctggcgcg tctcggcgtc
     2941 gcgcggctgg tagcgatagt tctgcacgtg cagctcctgc tggcgctgct cctgcgggta
     3001 gcggttcccc gagtactggc gctggtacgt gggcagcggc gcggcgcgcg gagcggagcg
     3061 atggtcccaa tggtcccagc ccgtgcggcg ctgctcccat tcgcggcccc agtgatcgcc
     3121 ccagcggggc gacagatcgg cgcgccatcc accgaagaat gccgggggat gccggtagta
     3181 gcgcaccggc acgcgcaata cgtagagcgg aacgagctcc ggggcgacga ggtgccacgg
     3241 gccgttgaac caggggctcg agtaccactg gtcgccg
//
LOCUS       DASVII010000498         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_274743,
            whole genome shotgun sequence.
ACCESSION   DASVII010000498 DASVII010000000
VERSION     DASVII010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274743"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..>1830)
                     /locus_tag="VF309_06495"
     CDS             complement(122..>1830)
                     /locus_tag="VF309_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7466270.1"
                     /translation="EEVTEDVVDAILEEASKLATQVLSPLNAVGDRQGATWKDGAVTT
                     PPGFKEAYAQYVNAGWGNIQSPEKYGGQGLPHLLATPIEEMWGAANLAFKLCPMLTQG
                     AIEAISHVGPDSLREKFLPNMVSGKWTGTMNLTEPQAGSDLSLVRSKATPQADGTYRI
                     KGQKIFITYGEHDYTENIVHLVLARIDGAPEGVKGISLFAVPKFHVKADGSIGERNDV
                     KCVSIEHKLGIHASPTAVMAYGEKDGATGYIVGEPHRGLEYMFIMMNAARLSVGLEGV
                     AVGERAYQRALAWSRERLQGKPIGVQGARTAPIIQHPDVKRMLLTMKSTTEAMRALAY
                     WTSALLDRARKHPDENERRRAQAMVDLLIPVVKGWSTEMGIEVATLGIQVHGGMGFIE
                     ETGAAQHLRDARITTIYEGTTGIQANDLVGRKMGREEGRTALALIAEIEKLLPQLAAS
                     SDANLKAIGSALAAAVEHLRETVQWVSKTFPSNPAAVAAGSVYVLKLMGVTLGGWMLA
                     RGAEIASRQIAKGEGDASFLKSKILTARFFADHVMSQTSSLSSAAIHGSESVLAVDEA
                     ML"
BASE COUNT          273 a          626 c          588 g          343 t
ORIGIN      
        1 gttgcgtctt gtcgccgatc tccccgagcg cgacgagacc agtggagacg agaaatggtg
       61 tcaggtacga atttccgagg agcggctccg tcatcggaaa ttcgtacctg acaccatttc
      121 tctagagcat cgcttcgtcg accgcgagga cgctttccga tccgtggatc gcggccgagg
      181 acagcgacga cgtctgcgac atcacgtggt cggcgaagaa tcgcgcggtg aggatcttgg
      241 acttgagaaa gctcgcgtcg ccttcgccct tggcgatctg gcgggatgcg atctcggcgc
      301 cccgcgcgag catccatccg ccgagcgtca cgcccatgag cttcagcacg tacacggagc
      361 ccgcggccac cgcggcagga ttcgagggaa acgtcttcga gacccactgc accgtctcgc
      421 gcagatgctc caccgccgcg gcgagcgcgg aaccgatcgc cttcaggttc gcgtccgacg
      481 acgccgcgag ctgcggcagc agcttctcga tctcggcgat cagcgcgagc gccgtgcgcc
      541 cttcctcgcg ccccatcttg cgcccgacga ggtcgttcgc ctggatgccg gtcgtgcctt
      601 cgtagatcgt cgtgatgcgc gcatcgcgaa ggtgctgcgc ggcgcccgtc tcctcgatga
      661 atcccatgcc gccgtgcacc tggatgccga gcgtggcgac ctcgatgccc atttcggtgg
      721 accagccctt cacgacggga atcagcaagt ccaccatcgc ctgggcgcgg cggcgttcgt
      781 tctcgtcggg atgcttgcgc gcgcggtcga gaagcgccga ggtccagtaa gcgagagcgc
      841 gcatggcctc ggtcgtggac ttcatcgtga ggagcatccg cttaacatcg gggtgctgga
      901 tgatgggcgc ggtcctcgct ccctgaacgc cgatgggctt tccctggagg cgctcgcgcg
      961 accatgccag cgcgcgctgg tacgcgcgct cgcccacggc cacgccctcc agcccgaccg
     1021 agagccgcgc cgcgttcatc atgatgaaca tgtactcgag gccgcgatgg ggctcgccca
     1081 cgatgtatcc ggtggcgccg tccttctcgc cgtacgccat caccgcggtc gggctcgcgt
     1141 ggatgcccag cttgtgctcg atggagacgc acttcacgtc gtttctctcg ccgatcgagc
     1201 cgtcggcctt cacgtggaac ttgggcaccg cgaagagcga gatgcccttc acgccctccg
     1261 gcgcgccgtc gatgcgcgcg aggaccaggt gcacgatgtt ctcggtgtag tcgtgctcgc
     1321 cgtaggtgat gaagatcttc tggcccttga tccggtaggt gccatcggct tgcggcgtgg
     1381 ccttggagcg caccagcgaa agatcgcttc ccgcctgggg ctccgtcagg ttcatcgtgc
     1441 cggtccactt tcccgagacc atgttcggca ggaatttctc gcgcagggaa tcgggaccca
     1501 cgtggctgat cgcctcgatc gcgccctgcg tgagcatcgg gcagagcttg aacgcgagat
     1561 tcgccgcgcc ccacatttcc tcgatcggcg tggcgagcag gtgcggcagc ccctgcccgc
     1621 cgtatttctc cggcgactgg atgttgcccc acccggcatt cacgtactgc gcgtaggcct
     1681 ccttgaatcc cggcggcgtg gtgacggcgc cgtccttcca cgtcgcgccc tggcgatccc
     1741 cgacggcgtt gagcggcgag agaacctggg tcgcgagctt gctcgcctcc tcgaggatcg
     1801 cgtcgaccac gtcctcggtg acctcttccc
//
LOCUS       DASVII010000499         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_239092,
            whole genome shotgun sequence.
ACCESSION   DASVII010000499 DASVII010000000
VERSION     DASVII010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239092"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF309_06500"
     CDS             complement(<1..287)
                     /locus_tag="VF309_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7466271.1"
                     /translation="MTTLVCGSLAFDTIMVFPDQFKKLILPDQIHILNVSYMVPDMRR
                     EYGGTAGNIAYNLKLLGEECHLMATVGHDFGPYEERLEGLSISSRHVLTLE"
     regulatory      459..547
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            561..>2741
                     /locus_tag="VF309_06505"
     CDS             561..>2741
                     /locus_tag="VF309_06505"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HEX7466272.1"
                     /translation="MAQAPSGNVIAIGDARARGRLSRKESADMLAGCRELALTQMARA
                     LSGMLDRVEYDLFELAEKALDRESQNMYLDARAQARDKRSAIESAFGRHFVELFNRKC
                     GDQTPVPVAARVGELSLVNEEDLEETLAMSEMSSKLKASCEGELMALSQRMGFLLERP
                     ELEDDANPMSPAAICAALRNACNQIEAGQKVRMTLLRQLESYAEGEVQRIYHDMNSHL
                     VERRILPDVRPGVPRAMIAAASKPAPKRAAGQGAGHGTAPAQPGGQVADGDILAALAQ
                     LLGPTGPAQGSGSAQQGGSPAGMASWPTAPGSASAQPTVPSSFVSELTRMHREPASGM
                     EDGALMNVVRRIKAAPQSATLGTVDAMTIDIIAMLFDNIFEDSHIPSSAKALLGRLQI
                     PTLKVALLDKSFFSSKAHPARRLLDLLAQSAIGVDEGSSRESPALAMIEGVVDRVLAE
                     FETDLALFEEMAQRVAAFIEQQKSAEGAIVERSARLIEERERDEIAREVAEQEIGRRL
                     ESRAGVPPAVREMLNETWVRALARVHRSEGEGSAAWQAMLLGMEDLLWSVEPKVTPDD
                     RKRLITMLPGMLRTLHEGLVRGELDAKGRGAFLGVLVDCHAMAVKAGMRGLVAVAEPA
                     PAIVRQDPSIERAMIPAGGVQVEEIRLRAPRGSSVRNVFTRTGIWSNLQRGTWVEFAR
                     EGGGGGTRARLTWISPNKGVYLFTNPLSPNAAVSISPEAPAQQMR"
BASE COUNT          476 a          889 c          950 g          426 t
ORIGIN      
        1 tcttccagcg tgagcacgtg gcggctggaa atggacagcc cctccaggcg ctcttcgtac
       61 ggcccgaagt cgtggcccac ggtcgccatg aggtggcatt cctcgccgag gagcttcagg
      121 ttgtacgcga tgtttcccgc cgttccgccg tattcgcgcc gcatgtccgg caccatgtac
      181 gaaacgttga ggatgtggat ctggtcggga aggatcagct tcttgaactg gtccgggaac
      241 accatgatgg tgtcgaaggc gagggagccg cagacgaggg tggtcatggc gggattatat
      301 gggctgaggc gtccttccca ggtcgacatg cggcatccaa acgccgacga gcggcattga
      361 ccattgcaaa cgtaacagcg aagctgagcc tgctgaatcg ggtttcgagg ccggcgttcc
      421 cattgaccgg cccaaggtaa tccctgtcgg cgcacgtttc tgaaacaggg caaacccatc
      481 gaaaggtggg gacgcaaaat ttccggtcta aggggcttag cagccctacg acagcgggat
      541 tgccagaaag gcggtgcacc atggcgcaag ctccatccgg caatgtgatc gccatcggcg
      601 atgcacgtgc gcggggacga ctgtcccgca aggaatcggc ggacatgctc gccggttgcc
      661 gcgagctcgc cctcacgcag atggcgcggg cgctctccgg aatgctcgat cgggtcgagt
      721 acgatctctt cgagctcgcc gaaaaggcgc tcgaccgcga atcccagaac atgtatctcg
      781 acgcgcgcgc ccaggcgcgc gacaagcgct cggcgatcga gtcggccttc ggccggcatt
      841 tcgtcgagct cttcaatcgc aagtgcggag atcaaacgcc cgtcccggtc gcggctcgcg
      901 tgggggagct ctccctggtc aatgaagagg atctcgagga aaccctcgcg atgagcgaga
      961 tgtcgagcaa gctcaaggcc tcgtgcgagg gagagctcat ggcgctgagc cagcgaatgg
     1021 gcttccttct cgagaggccc gagctcgagg acgacgcgaa tcccatgagc cccgccgcca
     1081 tctgcgccgc gctgaggaat gcgtgcaacc agatcgaggc gggacagaag gtgcgcatga
     1141 cgctccttcg gcagctcgaa agctacgccg aaggcgaggt gcagcgcatt taccacgaca
     1201 tgaattccca cctcgtcgag cgccgcatcc tgcccgacgt gcgcccggga gtgccgcgcg
     1261 cgatgatcgc ggccgcgtcc aagcccgctc cgaagagggc cgcgggtcaa ggtgcgggac
     1321 atggcacggc gccggcgcag cccgggggac aggtggccga cggcgacatt ctcgcggcgc
     1381 tcgcgcagct cctggggccc acgggtcccg cgcaaggatc gggatcggcg caacagggcg
     1441 gctcgcctgc ggggatggct tcgtggccga ccgcgcccgg gtccgcatcg gcccagccga
     1501 cggtgccttc ttcgttcgta agcgagctca cgcgcatgca tcgcgagccc gcgtcgggga
     1561 tggaggatgg ggcgctcatg aacgtggtga ggcgcatcaa ggccgcgccc cagagcgcca
     1621 cgctcgggac ggtcgacgcg atgacgatcg atatcatcgc gatgctcttc gacaacatct
     1681 tcgaggattc gcacatcccc tcgagcgcga aggcgctgct gggccgcctg cagattccca
     1741 ctctcaaggt cgcgctcctc gacaagagct tcttttcctc gaaggcgcac ccggcgcgcc
     1801 ggctcctgga cctgctcgcg cagtccgcca tcggggtgga cgaggggagc tcgcgcgaaa
     1861 gccccgcgct cgcgatgatc gagggagtgg tcgatcgcgt gctcgccgaa ttcgagaccg
     1921 acctcgcgct cttcgaagag atggctcagc gcgtcgccgc gttcatcgag cagcagaaaa
     1981 gcgccgaggg cgccattgtc gagcgatcgg cgcgcctgat cgaggagcgc gagcgcgacg
     2041 agatcgcgcg cgaggtcgcc gagcaggaga tcgggcgccg cctcgagtcg cgcgcgggcg
     2101 tgcctccggc ggtgcgggag atgctcaacg aaacgtgggt gcgcgcgctc gcgcgcgtgc
     2161 accgttccga aggcgagggc tcggccgcgt ggcaggcgat gctcctcggg atggaagacc
     2221 tgctgtggag cgtcgagccg aaagtcaccc cggacgaccg caagcgcctc atcacgatgc
     2281 ttccgggcat gctgcgcacg ctgcacgaag gcctggtgcg cggcgagctc gacgcgaagg
     2341 gccgcggcgc gttcctcggc gtgctcgtcg actgccacgc gatggcggtc aaggcgggga
     2401 tgcgcggtct cgtggccgtc gcggaaccgg cgccggccat cgtgcgccag gacccgtcga
     2461 tcgagcgcgc catgatcccc gcgggcggcg tccaggtcga agagatccgg ctgcgcgcgc
     2521 cgcgcggctc gtcggtgcgc aacgtgttca cgcgaaccgg catctggagc aacctccagc
     2581 gcggaacctg ggtcgagttc gcacgtgaag gcggcggtgg cggcacgcgc gcgcgcctga
     2641 cctggatcag ccccaacaag ggcgtctatc tcttcaccaa tccgctttca cccaacgcgg
     2701 cggtttcgat ttctcccgag gcgcccgcgc agcagatgcg c
//
LOCUS       DASVII010000500         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_166648,
            whole genome shotgun sequence.
ACCESSION   DASVII010000500 DASVII010000000
VERSION     DASVII010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166648"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VF309_06510"
     CDS             complement(<1..1468)
                     /locus_tag="VF309_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX7466273.1"
                     /translation="MSTPFAAHPSLTHNRLLTLLVVAFVVVAGSAMLRTSTTFDEIVF
                     SAVGARGIATGDFTFVNDHPRLAQYMFGLPVYLSIDNYPSEAGQWLTRYHYARALIWS
                     VGNNSEQVVMVARLVGLAFGTLTVLATYFLSRSHLGAGAALFAAALVAFLPDVLAHSG
                     VAYNDIPLAFGVLVSVYALDSAVRNPGPARVALAALACALTVCIKYSGVIVGPVLIGL
                     LLLEALSGRWRDRKWRRSILLGVPLFMLVGYATIVLIYLGDWRLADFFSGMREIAQVS
                     SAGRVAFLLGERNVGGWWYFFPVALALKTPLALHVLMLLGVVGAWAAARGGRWREWLV
                     HPARAPALGAVLFLIAVLGSGINIGSRHALPALPLVCILVAQGIAPIWDRGRMAVRGA
                     LAVVFAAYLLSTLSHYPYFLSYLSEYARGRALHETLVDSSTDWGQGLVALRAYMREQN
                     IDQVALAYFGSALPEGYGIRYVALPSFLDLPPQAESPAA"
     gene            1584..>2019
                     /locus_tag="VF309_06515"
     CDS             1584..>2019
                     /locus_tag="VF309_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7466274.1"
                     /translation="MTATRSAIRKAVVASIAAIVPWVPSGASAQDYPTKAVRVIVPYA
                     AGGGADILARLVGQELSQRLKQAVIVENQGGGANTIGMRTVVTSPKDGYTLGLATPVF
                     LMTPSLMKNHPYDPPRDFTPVAMIGFTPLVLVVHPSVPAKTTK"
BASE COUNT          407 a          674 c          668 g          270 t
ORIGIN      
        1 gcgccgcagg actctcggcc tgcggcggca gatcgagaaa gctcggcaac gcgacatagc
       61 ggatgccata gccctccggc agcgccgatc cgaaataggc gagcgcgacc tggtcgatgt
      121 tttgctcgcg catgtaagcg cgcaacgcga ccagtccctg gccccaatcg gtgctcgaat
      181 cgaccagcgt ctcgtgaagc gcgcggccac gcgcgtactc gctcagatac gacaggaaat
      241 acggatagtg cgaaagcgtc gaaaggaggt acgccgcgaa gaccaccgca agcgccccac
      301 gcaccgccat gcggccgcgg tcccagatgg gggcaatgcc ctgcgcgacc aggatgcaga
      361 cgagcggcag cgccggaagc gcatggcgcg agccgatgtt gattccggag ccgagcaccg
      421 cgatcaggaa gagcaccgcg cccaacgcgg gcgcgcgcgc cggatgaacc agccattcac
      481 gccagcgccc tccgcgcgcc gcggcccatg cgccgacgac gccgaggagc atgaggacat
      541 gcagcgcgag gggcgtcttg agcgccagcg cgacggggaa gaagtaccac cacccgccga
      601 cattgcgctc gcccagcagg aatgcgactc gccccgccga ggagacttgc gcgatctcgc
      661 gcatgccact gaagaaatcc gcgaggcgcc agtcgccgag atagatgaga acgatcgtgg
      721 cgtaacccac cagcatgaat agcggcacgc cgaggaggat cgagcgccgc cacttgcggt
      781 cgcgccagcg tcccgacagc gcctcgagca gaagcaatcc gatcagcacc ggtcccacga
      841 tgacgccgct gtacttgatg cataccgtga gcgcgcaggc gagcgccgcg agcgctaccc
      901 gggcggggcc ggggttgcgc accgcactgt ccaacgcata cacgctcacg agcaccccga
      961 aggcgagcgg aatatcgttg taggcaacgc ccgaatgggc aaggacgtcg ggaaggaacg
     1021 cgaccagcgc cgccgcgaag agcgccgccc cggcgcccag atggctcctg gacaggaaat
     1081 aagtggcgag caccgtgaga gtcccgaacg cgagccccac cagccgcgcg accatcacca
     1141 cctgctcgga attgttgccc acgctccaga tgagcgcgcg cgcgtagtgg tagcgcgtca
     1201 gccactgccc cgcctcggag ggatagttgt cgatcgacag gtaaaccggc aagccgaaca
     1261 tgtattgcgc gagcctggga tgatcgttca cgaacgtgaa atcgcccgtg gcgatcccgc
     1321 gcgcgccgac ggccgagaac acgatttcgt cgaatgtggt cgacgtgcgc agcatcgcgc
     1381 ttcccgcgac cacgacaaat gccaccacga gcagcgtgag taggcggttg tgcgtgaggc
     1441 tcgggtgcgc tgcgaacggg gtgctcatgg ccgctgacag catacccgcg cttggggcgc
     1501 gcgcctcaaa ttgaccggtc cggcattcca tgcttaactg cgttgcgcgg aatgaatgga
     1561 acgatgttcc gcataaagga atcatgacgg cgacccgtag cgccattcgc aaggcagtcg
     1621 ttgcatcgat cgccgcgatc gtcccgtggg tcccatcggg cgcttcggcg caggactatc
     1681 ccaccaaggc cgtgcgcgtg atcgtgccct acgcggcggg cggcggcgcc gatatcctcg
     1741 cgcgcctcgt ggggcaggag ctctcgcagc gcctgaagca ggccgtgatc gtcgagaacc
     1801 aggggggcgg cgccaacacc atcggcatgc gcaccgtcgt gacgtcgccg aaggacggat
     1861 acacgctggg cctcgcgacg ccggtcttcc tgatgacgcc ctcgctgatg aaaaaccatc
     1921 cgtacgaccc gccgagggat ttcacgccgg tggcgatgat cggattcacg ccgctggtgc
     1981 tggtcgtgca tccgagcgtt cccgcgaaga ccacgaagg
//
LOCUS       DASVII010000501         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_346903,
            whole genome shotgun sequence.
ACCESSION   DASVII010000501 DASVII010000000
VERSION     DASVII010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346903"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VF309_06520"
     CDS             <1..815
                     /locus_tag="VF309_06520"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX7466275.1"
                     /translation="PTHAYLGLSPGLDFETRLTAKVNAAEKLREELAKPGYKCEPLTV
                     GVNTDAYQPIERQQKITRSILEVASETNHPVSLITKSALIERDLDLLAPMAKKRLVAV
                     TFSITTLDHEISRRMEPRTSAPARRLEAIRRLTAAGIPVNVNVAPVIPFLTDSELENI
                     MEAAANAGAVSAGYVLIRLPWEVRPIFKEWLEKHFPLKAEHVMSRIRDMREGRENDPE
                     FGTRMVGKGTLAELLSQRFRKAVQRFGLDRDVHDLDCSLFLPPSLSGQQSLF"
     gene            937..>2939
                     /locus_tag="VF309_06525"
     CDS             937..>2939
                     /locus_tag="VF309_06525"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX7466276.1"
                     /translation="MSALPISLEDKYTLASGRVFLNGTQALVRLAMIQRERDAAQGLN
                     TAGFISGYRGSPLGGFDQALWKAKDHLRQSHVFFTPGVNEELAATAVWGSQQVQLFEG
                     AKYDGVYAMWYGKGPGVDRSGDVFKHANAAGTAKHGGVLLLAGDDHACKSSTLPHQSE
                     HAFDAAMIPVLYPTGVQEIIELGLHGFAMSRYSGCYVAFKVISETVDSSASIEVNPDS
                     PRILLPQDFQLPATGVNIRWPDAPMEQELRLQHDKIYAALAYARVNRLNRITVDSPNP
                     RLGIIASGKSYLDVLQALEDLGIDADHAARIGIRLYKVGMPWPLEPNGVREFAQGLEE
                     ILVVEEKRQLIEYQMKEQLYNWRDDVRPKVIGKYDEHGEWEVHRSEWLLPAAGELTPA
                     MVARVIAQRIGKFYTSETIQARLKFLEAKEAALARPRDKVARIPYFCSGCPHNTSTRV
                     PEGSKALAGIGCHYMAIWIRPDQTMTFTQMGGEGVPWVGIAPFTETKHVFANLGDGTY
                     FHSGLLAIRAAAAAGVNITYKILFNDAVAMTGGQPVDGQLTVSMVTRQVLAEGVKRVV
                     VVTDEPEKYASVTDLAPGTMVYHRDELDAVQRELREITGTTVLVYDQTCAAEKRRRRK
                     RGKFPDPAKRVVINELVCEGCGDCGSKSNCLSIVPVETEFGRKR"
BASE COUNT          535 a          995 c          938 g          471 t
ORIGIN      
        1 gcccgacaca cgcctatctg ggtctttcgc ctggtctcga cttcgaaacg cgcctcactg
       61 ccaaggtcaa cgcggccgaa aagctgcgcg aggaactcgc gaagccgggc tacaaatgcg
      121 agccgctcac cgtcggcgtg aacaccgacg cgtaccagcc catcgagcgc cagcagaaga
      181 tcacgcgctc gattctcgaa gtggcatcgg agaccaatca tccggtatcg ctcatcacca
      241 agtcggcgtt gatcgagcgc gacctcgatc tcctcgcgcc gatggcgaag aagcgcctcg
      301 tcgccgtgac gttttcgatc acgaccctcg accacgagat ttcgcgaagg atggagccgc
      361 gcaccagcgc gccggccagg cgcctggaag ccattcgtcg actcactgcc gcgggcattc
      421 ccgtcaacgt gaacgtggcg ccggtgatcc cgttcctcac cgattccgag ctcgagaaca
      481 tcatggaggc cgcggcaaat gcgggcgccg tgagcgccgg ctacgtcctc atccgcctgc
      541 cctgggaagt gcgcccgatc ttcaaggagt ggctcgagaa gcattttccc ctcaaggccg
      601 agcacgtgat gtcgcgcatc cgcgacatgc gcgaaggccg cgagaacgac cccgagttcg
      661 gcacgcggat ggtgggcaag ggcaccctcg ccgagctcct ctcgcagcgc ttccgcaagg
      721 ccgtccagcg cttcggtctc gaccgggacg tgcacgacct ggattgcagt ctcttcctgc
      781 cgccctcact tagcgggcag cagtcgcttt tctgagcgaa acgtctctgg ctcggaagcg
      841 gtccaaccgc ttataatttg cggctatttc cggccgagga cacaatccgg gggccggaag
      901 catcacgccc caaagcaccc cttttgagga gcatgaatgt ccgcattgcc catctcgctg
      961 gaagacaagt acaccctcgc ttccggccgc gtctttctca acggcaccca ggctctcgtg
     1021 cgcctcgcga tgatccagcg cgagcgcgat gccgcccagg ggctgaacac cgcgggattc
     1081 atctcgggct accgcggctc gccgctcggc ggcttcgacc aggcgctgtg gaaggcgaag
     1141 gaccatctga ggcagagcca tgtcttcttc acgccgggcg tgaacgagga gctcgccgcg
     1201 accgccgtgt ggggctccca gcaggtgcag ctcttcgagg gcgcgaagta cgacggcgta
     1261 tacgccatgt ggtacggcaa ggggccgggc gtggaccgca gcggcgacgt gttcaagcac
     1321 gccaacgccg cgggaaccgc caagcatgga ggggtcctcc ttctcgcggg cgacgaccat
     1381 gcgtgcaagt cctcgacgct tccgcaccag agcgagcatg cattcgacgc cgcgatgatt
     1441 ccggtgctgt acccgaccgg cgtgcaggag atcatcgagc tgggcctgca cggcttcgcg
     1501 atgtcgcgct actccggctg ctatgtcgcc ttcaaggtga tctcggagac ggtggactcg
     1561 tcggcatcga tcgaggtgaa tcccgacagc ccccggattc tccttcccca ggatttccag
     1621 cttccggcca cgggcgtgaa catccgctgg cccgacgccc cgatggagca ggagcttcgc
     1681 ctgcagcacg acaagatcta cgcggcgctc gcgtacgcgc gcgtgaaccg cctgaatcgc
     1741 atcacggtcg actcgccgaa cccgcgcctg ggcatcatcg cctcgggcaa gagctacctc
     1801 gacgtgctcc aggcattgga ggatctcggc atcgacgcgg accacgcggc gcgcatcggg
     1861 attcgcctgt acaaggtcgg catgccctgg cccctcgagc ccaacggcgt ccgcgagttc
     1921 gcgcagggcc tcgaggagat cctcgtggtc gaggaaaagc gccagctgat cgaataccag
     1981 atgaaggagc agctctacaa ctggcgcgac gacgtgcgcc cgaaggtgat cggcaagtac
     2041 gacgagcacg gcgagtggga ggtgcatcgc agcgagtggc tccttcccgc ggcgggcgag
     2101 ctcacgcccg cgatggtcgc gcgcgtcatc gcgcaacgca tcggcaagtt ctatacgtcg
     2161 gagacgatcc aggcgcgcct gaagtttctc gaggccaagg aagcggcgct cgcgcgcccg
     2221 cgcgacaagg tcgcccgcat tccgtacttc tgctccggat gcccgcacaa cacctcgacg
     2281 cgcgtgcccg aaggctcgaa ggcgctcgcc ggcatcggct gccactacat ggcgatctgg
     2341 atccgtcccg accagaccat gaccttcacg cagatgggcg gggagggcgt gccatgggtg
     2401 ggcatcgccc ccttcaccga gacgaagcac gtgttcgcga acctcggcga cgggacgtat
     2461 ttccacagcg gcttgctcgc gatccgcgca gccgcggcgg cgggcgtcaa catcacctac
     2521 aagatcctgt tcaacgacgc cgtcgcgatg accgggggcc aacccgtgga cggccagctc
     2581 accgtgtcga tggtcacgcg ccaggtgctc gcggagggcg tgaagagagt cgtggtggtc
     2641 accgacgagc ccgaaaagta cgcgagcgtg accgatctcg cgcccggcac gatggtgtat
     2701 caccgcgacg agctggacgc ggtgcagcgc gagttgcggg agattaccgg caccacggtg
     2761 ctcgtgtacg accagacttg cgccgcggag aagcgccgcc gccgcaagcg cggcaagttc
     2821 cccgatcccg ccaagcgcgt cgtcatcaac gagctcgtgt gcgagggctg cggcgattgc
     2881 ggctcgaagt ccaactgcct ctcgatcgtt ccggtcgaga ccgagttcgg tcgcaagcg
//
LOCUS       DASVII010000502         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_131227,
            whole genome shotgun sequence.
ACCESSION   DASVII010000502 DASVII010000000
VERSION     DASVII010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131227"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..560)
                     /locus_tag="VF309_06530"
     CDS             complement(105..560)
                     /locus_tag="VF309_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7466277.1"
                     /translation="MPSYKHILVPTDGSKLSLKAAKEAAALAKGLKAKMTAIYVIAPY
                     MPTLDAEGAIGMRHGYDQKAYEAASEKFAQSATAKIAATAAAINVECAQIHVTDPAPW
                     DGIIRAAAKNKCDLIVMSSHGRGSLSGLLLGSETVKVLAHSKTPVLVCR"
     gene            complement(572..1360)
                     /gene="fnr"
                     /locus_tag="VF309_06535"
     CDS             complement(572..1360)
                     /gene="fnr"
                     /locus_tag="VF309_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEX7466278.1"
                     /translation="MQANTLRNEPSVTRHMKVVARNEVPKRRISGLATVPTHCTTCNL
                     RELCLPCGLAASDAELLGDLAYTRKRVKRGESLYRSGGAFDSLYAVRSGFCKSSVVLE
                     DGRDQVTGFHMAGEVLGMDGIGTDSHSSDVIALEDSEVCIIPYTHLEEPRLQRQLHKV
                     MSRELVRDQGVMMLLGTMKAEERLAAFLLNLSTRFLARGFSHSEFHLRMTREEIGSYL
                     GLSLETVSRLFSRFQDDGLITVQQKHICILDVTGLRSVMVHEND"
     gene            complement(1389..1775)
                     /locus_tag="VF309_06540"
     CDS             complement(1389..1775)
                     /locus_tag="VF309_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7466279.1"
                     /translation="MQTRRTSPVFIVDDSPLIRTRLAEMVSAMSGMRLVGEAASASEA
                     IAGIKRTQPDSVLLDLNLMGRTGLDVMRAIRPHSPGIAFVVLTNHSEPQYRRAALEAG
                     AAYFLDKSSELGRVSGVLAEIASTRH"
     gene            1953..>2193
                     /locus_tag="VF309_06545"
     CDS             1953..>2193
                     /locus_tag="VF309_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466280.1"
                     /translation="MDKPGITSRIDAITRIPIRYLAPRLPAPRAVKIELTSQCNYRCG
                     FCAHRLRMKTRGEMDRGFFERVVGEMVDAGVEELGV"
BASE COUNT          331 a          698 c          787 g          377 t
ORIGIN      
        1 tgggcctgtg cgcgaagctc gggttcgcct ccaggcccga ggccgacgac ccgcagatgc
       61 gcaacgtgtc gctcgagctc gcgtccgcga atcggcgata ggcctcagcg gcagacgaga
      121 accggcgttt tcgagtgggc gaggaccttc acggtctcgc tgcccagcag gagccccgaa
      181 aggctgccgc gcccatggga ggacatgacg atgaggtcgc atttgttctt cgccgcggcg
      241 cggatgatgc cgtcccaggg ggcgggatcg gtcacgtgga tctgggcgca ctccacgttg
      301 atcgccgccg cggtggccgc gatcttcgcg gtcgcggact gcgcgaactt ctcgctcgcg
      361 gcctcgtagg ccttctggtc gtagccgtgc ctcatgccga tggcgccctc ggcgtcgagg
      421 gtcggcatgt agggggcgat cacgtagatc gcggtcatct tggccttcag gcccttcgcc
      481 agcgccgcgg cttccttcgc cgccttgagc gagagcttcg agccgtcggt gggcaccagg
      541 atgtgcttgt acgaaggcat ggagtctccc ttcagtcgtt ctcgtggacc atcacggagc
      601 gcaggcccgt gacgtcgagg atgcagatgt gcttctgctg gacggtgatg aggccgtcgt
      661 cctggaagcg cgagaagagg cggctcacgg tctcgagcga caggcccagg tagctgccga
      721 tctcctcgcg cgtcatgcgg aggtggaatt ccgagtgcga gaagccgcgc gcgaggaagc
      781 gcgtcgacag gttcaggagg aacgcggcga ggcgctcctc ggccttcatc gtgccgagca
      841 gcatcatcac gccctggtcg cgcaccagct cgcggctcat caccttgtgc agctggcgct
      901 gcagccgcgg ctcctcgagg tgcgtgtagg gaatgatgca gacctcgctg tcctcgagcg
      961 cgatcacgtc cgagctgtgg ctgtcggtgc cgatgccgtc catgccgagg acctcgccgg
     1021 ccatgtggaa gcccgtcacc tggtcgcgtc cgtcctcgag cacgacgctc gacttgcaga
     1081 agccgctgcg gaccgcgtag agggagtcga aggcgccgcc ggaacggtag agcgactcgc
     1141 cgcgcttcac gcgcttgcgg gtgtaggcga ggtcgccgag gagctccgcg tccgaggccg
     1201 cgaggccgca cggaaggcac agctctcgca ggttgcaggt cgtgcagtgc gtcgggaccg
     1261 tggcgaggcc ggagatgcgc cgtttcggaa cctcgttgcg cgcgacgacc ttcatgtggc
     1321 gcgtgaccga cggctcgttg cggagagtgt tcgcttgcat ggggagctcc ttctcatggc
     1381 gcgtgtgctc aatgacgggt ggaagctatt tcggcgagga cgccgctcac gcggcccagc
     1441 tcgctggatt tgtcgaggaa gtaggccgcg ccggcttcga gggcggcccg gcgatactgc
     1501 ggctccgagt ggttggtcag gaccacgaag gcgattccgg gcgaatgggg ccggatcgcg
     1561 cgcatgacgt cgagcccggt gcgtcccatg aggttcaggt cgaggaggac cgaatcgggc
     1621 tgggtgcgct tgattcccgc gatcgcctcg ctcgcgctcg cggcctcgcc cacgaggcgc
     1681 atgccgctca tcgcgctgac catctcggcg agccgggtgc ggatcagggg ggagtcgtcg
     1741 acgatgaaca cggggctggt tcgccgcgtt tgcatggaag ccattctcga cgccgatcga
     1801 ggcgcgcaaa atcggcggcg gctgaagcgc gcctcggagc gggcgcggcg cgagcgccgg
     1861 gtgcgctcct agcgagcgcc taggaatagt cctagtgatc aatccttgat ccggcgcaag
     1921 cccgcggagg gggctgtcca cagaattggc ccatggacaa acccggcatc acctcgcgca
     1981 tcgacgcgat cacgcggatc ccgatccgtt acctcgcccc gcgcctcccg gcgccgcgcg
     2041 ccgtcaagat cgagctcact tcgcaatgca actatcgctg cggcttctgc gcccatcgcc
     2101 tgaggatgaa gacccgcggc gagatggatc gcggcttctt cgagcgcgtc gtcggcgaga
     2161 tggtcgatgc cggcgtcgag gagctgggcg tct
//
LOCUS       DASVII010000503         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_634327,
            whole genome shotgun sequence.
ACCESSION   DASVII010000503 DASVII010000000
VERSION     DASVII010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634327"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VF309_06550"
     CDS             complement(<1..370)
                     /locus_tag="VF309_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466281.1"
                     /translation="METTWRDVGRLEACHRRLARGALAYVSFIPGQTWRETISACVAV
                     RAAGFEPVPHIPARELASAEALDNLMAQLAARVGVRRVLLVGGDRDVPAGPFAQSLDV
                     LRSGVLARNGIREVAVAAHPE"
     gene            complement(432..1922)
                     /locus_tag="VF309_06555"
     CDS             complement(432..1922)
                     /locus_tag="VF309_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466282.1"
                     /translation="MGCFNAGNPAEEPMQAVPHKPAPASRARRVNNDRLLADIAAFCQ
                     AAGMAESTFGRRVANDGKLVSRLRAGGRLTTQTVERIRSFISTARDGRGGESATDTDA
                     DAAPASRSAKLTTRAPPADSHTRFRFYDNRQKYLLFVSTCTEKWMVAQRIEAELANIK
                     PQPPAMRVFDAGTGDGTVLTRLMRSMHDRFTNVPFYIVGKEISLEDVRLALEKMPDRF
                     HEHPSTVLVMTNMYYSEAPWLRPNSLAAASGLVWHELALRGSTAGQFERQITDLQGFL
                     SENWTAKISKTTGNPIYERPVVLVIYRDDHKLLLDGVRPRQGAVRADFDLVIASQPYR
                     ARATTQFKASKVIAPLARALGPGGRLIGIHSCGADPGLEIIQRVWPGENPFLMDRHEL
                     LKAAKHALGAEARALSFNAYADQRSRFRYRMHTLPSEINSSIGTSTLLAAWNAAIYVA
                     QIEDGRLEAVMGDRRYLEATRDVLQQSGGLWFQNESYVISRHRFAG"
BASE COUNT          297 a          658 c          695 g          321 t
ORIGIN      
        1 cctcgggatg cgccgccacc gcgacctcgc ggatcccatt gcgcgccagc actccgcttc
       61 gcagtacgtc gagggattgc gcgaagggcc cggccggcac gtcgcggtcg ccgcccacca
      121 gcagcacgcg ccggacgcca actcgcgcgg ccaactgcgc catcaggtta tccagcgctt
      181 ccgcgctggc gagctcgcgt gccgggatgt ggggcaccgg ctcgaatccc gccgcgcgca
      241 cggcgacgca ggccgatatc gtctcgcgcc acgtctgccc cgggatgaag ctcacgtacg
      301 cgagcgcgcc gcgagcgagg cgccggtggc acgcttcaag gcggccgacg tcgcgccacg
      361 tcgtctccac ggacgcacgc tgcgcgagcg ctgcgatcgc gcgggcttcg tccccgcacg
      421 ctcgcgcgcc gttatccggc gaaccggtgg cgcgagatga cgtaggactc attctggaac
      481 cagaggccgc cgctttgctg cagcacgtcg cgcgtggctt cgaggtagcg ccgatcgccc
      541 atcaccgcct cgaggcggcc gtcctcgatc tgggcgacat agatcgcggc gttccatgcg
      601 gcgagaagcg tggaagtgcc gatcgaggag ttgatctcgg aaggcagcgt gtgcattcga
      661 tagcggaaac gcgagcgctg gtcggcgtac gcgttgaagc tgagggcgcg cgcctccgcc
      721 ccgagcgcgt gcttcgcggc cttgaggagc tcgtggcgat ccatgaggaa gggattttcg
      781 ccgggccaca cacgctggat gatttcgagg ccgggatcgg cgccgcagga gtggatgccg
      841 atcaatcgac cacccgggcc gagcgcgcgc gcgagcggcg cgatgacctt cgacgccttg
      901 aactgcgtgg tcgcgcgcgc gcggtacggc tgcgaggcga tcaccaggtc gaagtcggcg
      961 cgcacggcgc cttgcctcgg gcgcacgccg tcgagcagca gcttgtggtc gtcgcgatag
     1021 atcacgagca cgaccgggcg ctcgtagatc ggattgcccg tcgtcttgct gatcttggcc
     1081 gtccagtttt ccgagaggaa tccctgcagg tcggtgatct ggcgctcgaa ctgcccggcg
     1141 gtgcttcccc tcaacgcgag ctcatgccac accaggccgc tcgccgccgc gagggaattg
     1201 ggcctgagcc acggcgcttc cgagtagtac atgttcgtca tcaccagcac ggtggacgga
     1261 tgctcgtgga aacggtccgg catcttctcc agcgcgaggc gcacgtcctc caggctgatc
     1321 tccttgccga cgatgtagaa ggggacgttg gtgaaccggt cgtgcatcga gcgcatgagg
     1381 cgcgtgagaa cggtgccgtc gccggtgccc gcgtcgaaca cacgcatcgc cggcggctgc
     1441 ggcttgatgt tcgcgagctc ggcctcgatc cgctgcgcca ccatccactt ctcagtgcag
     1501 gtgctgacga acagcaggta cttctgtcgg ttgtcgtaga agcgaaagcg cgtgtgcgag
     1561 tcggccgggg gagcgcgcgt cgtcagcttc gcggacctcg aagctggggc cgcatcggcg
     1621 tccgtgtcgg ttgcggactc gccgccccgc ccgtcgcgag cagtggagat gaaggatcgg
     1681 atccgctcga ccgtctgcgt cgtgaggcgg ccgccggcgc gcaggcgcga gacgagcttt
     1741 ccgtcgttgg cgacgcggcg accgaaggtg ctctcggcca tgccggccgc ctggcagaac
     1801 gccgcgatgt ccgcgagcag ccggtcgttg ttcacgcggc gcgcgcgtga tgcaggcgcg
     1861 ggtttgtgcg gcacggcttg catgggctcc tccgccggat ttcccgcatt gaagcagccc
     1921 atggccgccc aacgcaagtg tgggcaggat agtgggatat atcccactag c
//
LOCUS       DASVII010000504         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_418969,
            whole genome shotgun sequence.
ACCESSION   DASVII010000504 DASVII010000000
VERSION     DASVII010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418969"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1827
                     /gene="leuS"
                     /locus_tag="VF309_06560"
     CDS             <1..>1827
                     /gene="leuS"
                     /locus_tag="VF309_06560"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7466283.1"
                     /translation="KTGLKVFTTRADTIMGITFAAVAAEHPLAAEAAKGNPALATFIE
                     ECRKGGVQEAEIATMEKKGMATGLHVRHPFTGDPIEVWVGNYVLMGYGEGAVMAVPGH
                     DERDFAFAKKYRLPIKQVIDVEGKPYSLDAWEAWYEEPGRNVDSGPYDGLTQAAAIEA
                     VATALEKAGLGRKRVQYRLRDWGISRQRYWGTPIPIVQCPKCGDVGVPDEQLPVILPE
                     DLVPDGTGSPLAKTPSFYETKCPKCAGPAKRDTDTLDTFVDSSWYFMRYACPDASTMV
                     DERAQYWMPLDMYIGGIEHAILHLLYARFWTKAMRDLGLVKIDEPFANLLTQGMVLNH
                     IFSRRAAAGGVEYFAPDEVEASMDTEGRITGAKLKSDGSAIDYEGIKTMSKSKRNGVD
                     PQEMVEKYGADTSRFYVMQANPPTDTMIWSDQSVAGSFKFLRGLWTLVHEQVTPGIVT
                     RYHEGELTSELAALRLKLHKTIEKVGDDYGRRLQFNTAIAAVREMLNLYETVKDRSPV
                     ARAVAQEVLEDAVLLLSPIVPHIATTLWRELRPGTELIDQPWPSVDAAALVQDSIELV
                     VQVNGKLRGHMHVSTTASREQIAAAAMLHEAVVKFAAGQTPKK"
BASE COUNT          349 a          579 c          627 g          272 t
ORIGIN      
        1 aaaaccggcc tcaaggtctt cacgacgcgc gccgacacca tcatggggat caccttcgcc
       61 gccgtagccg cggagcatcc gctggcggcc gaagccgcga agggaaatcc cgcgctcgcc
      121 acgttcatcg aggagtgcag gaagggcggc gtgcaggaag ccgagatcgc gacgatggag
      181 aagaagggca tggcgaccgg ccttcacgtg cgccatccct tcaccggcga tccgatcgaa
      241 gtgtgggtcg gaaactacgt gctgatgggt tacggcgagg gcgcggtgat ggcggtgccg
      301 ggacacgacg agcgcgattt cgccttcgcg aagaagtacc gcctccccat caagcaggtg
      361 atcgacgtcg agggaaagcc ctattcgctc gacgcctggg aggcgtggta cgaagagccg
      421 gggcgcaacg tcgactccgg cccatacgat ggattgacgc aggcggccgc gatcgaagcg
      481 gtcgcaaccg cgctggaaaa ggcggggctc ggaaggaagc gcgtccagta ccgccttcgc
      541 gattggggca tctctcgcca gcgctactgg gggacgccca tcccgatcgt gcaatgcccg
      601 aagtgcggcg acgtcggcgt cccggacgag cagttgccgg tgattcttcc cgaggacctc
      661 gtccccgacg gcaccggcag cccgctcgcg aagacgcctt ccttctacga gacgaagtgc
      721 ccgaagtgcg ccggccccgc gaagcgcgac acggacaccc tggacacctt cgtcgattcg
      781 tcgtggtact tcatgcgcta cgcgtgtccc gacgcgagca cgatggtcga cgagcgcgcg
      841 caatactgga tgccgctcga catgtacatc ggcggcatcg agcacgcgat cctgcacctg
      901 ctctacgcgc gcttctggac gaaggcgatg cgcgatctcg ggctggtgaa gatcgacgag
      961 ccgttcgcga acctgctcac ccagggcatg gtgctcaacc acatcttctc gcggcgcgcg
     1021 gccgcgggcg gcgtcgagta tttcgcgccc gacgaggtcg aagcctcgat ggacaccgag
     1081 ggcaggatta ccggcgcgaa gctgaaatcg gacggctcgg cgatcgacta tgaaggcatc
     1141 aaaacgatgt ccaagtcgaa gagaaacggc gtcgatcccc aggaaatggt cgagaagtac
     1201 ggcgcggaca cttcgcgctt ctacgtgatg caggcgaacc cgccgacgga cacgatgatc
     1261 tggtccgacc agagcgtggc cggctcgttc aagttcctgc gcgggctctg gacgctggtg
     1321 cacgagcagg tgacgccggg aatcgtcacg cgatatcacg agggcgagct cacctcggag
     1381 ctggcggcgt tgcgcctcaa gctgcacaag acgatcgaga aggtcggcga tgactacggg
     1441 cggcgcctgc aattcaacac cgcgatcgcc gcggtgcgcg agatgctgaa tctctacgag
     1501 accgtgaagg accgcagccc cgtggcgcgc gccgtggcgc aggaagtgct cgaggacgcg
     1561 gttctcctcc tgtcgcccat cgtgccgcac atcgcgacca ctctgtggcg cgagcttcgc
     1621 cccggcaccg agctcatcga ccagccctgg ccttcggtgg atgcggcggc gctggtgcag
     1681 gactcgatcg agctcgtggt gcaggtgaac gggaagctcc gcgggcacat gcacgtgagc
     1741 acgacggcga gccgcgagca gatcgccgcg gccgcgatgc tgcacgaggc ggtggtcaag
     1801 ttcgcggcgg ggcagacgcc gaagaag
//
LOCUS       DASVII010000505         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_23904, whole
            genome shotgun sequence.
ACCESSION   DASVII010000505 DASVII010000000
VERSION     DASVII010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23904"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VF309_06565"
     CDS             complement(<1..686)
                     /locus_tag="VF309_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7466284.1"
                     /translation="MPRYRDVTIVDAGEPKRIDLRIEVPLDDMSRLDDLIALPSGPAS
                     QAPVRPSIWSAIHPKLLALVRAHTSTLIFVNSRRLAERISGAVNELAGETLVRAHHGS
                     VASDQRKEIEDRLKMGTLRGLVATSSLELGIDMGAIDLVVQIESPPSVASGMQRVGRA
                     SHHVGGTSAAVIFPKYRGDLVACAAITRAMYAGKVEAVHYPRNPLDVLAQQIVATVAM
                     DPWDANALFTL"
     gene            750..>1522
                     /locus_tag="VF309_06570"
     CDS             750..>1522
                     /locus_tag="VF309_06570"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466285.1"
                     /translation="MLSGRYPSDEFAELRPRLTWDRVTHQLTSRQGARSVAVINGGTI
                     PDRGLFTVYLAGATRGARVGELDEEMVFERRAGDTIILGATTWRIDQITHDRVNVTPA
                     PGQPGKMPFWHGDSAGRPAEFGREIGEMTRELLATPRAAAFTRLVEQHSLDAGAAENL
                     LRYLEDQTAATGRVPSDRDIVIERCRDELGDWRVCVLTPFGSRVHAPWCMAVTAKLRA
                     ERGVEVETMWSDDGFVIRLPESEEPLETEWLLPAPAELK"
BASE COUNT          239 a          521 c          540 g          222 t
ORIGIN      
        1 agggtaaaga gggcgttggc atcccacggg tccatggcga ccgtggcgac gatctgctgg
       61 gccaggacgt cgagcgggtt gcgcgggtaa tggacggctt ccaccttgcc ggcgtacatg
      121 gcgcgcgtga tggcggcgca ggccaccaga tcgccgcggt acttggggaa gatcacggcg
      181 gcgctggtgc cgccgacgtg atggctggcg cggcccacgc gctgcatgcc gctggcgacg
      241 gagggcggcg attcgatctg caccacgaga tcgatggcgc ccatatcgat accgagttcg
      301 agcgaggagg tggccaccag gccgcgcagc gtgcccatct tgaggcggtc ctcgatttcc
      361 ttgcgctgat cggaggccac gctgccgtgg tgcgcgcgca ccagcgtttc gccggcgagc
      421 tcgttgacgg cgccggagat gcgctcggcg agacggcggc tgttgacgaa gatgagggtg
      481 gaggtgtggg cgcggaccag agcgagcagc ttcggatgga tggcgctcca gatggagggg
      541 cgcacggggg cctgcgaggc ggggccgctg ggcagggcga tgaggtcatc gaggcgcgac
      601 atatcgtcga gcgggacttc gatgcgcagg tcgatgcgct tgggctcgcc ggcatcgacg
      661 atggtgacgt cgcggtagcg gggcacggcg gaggcggatt cgaactcggc gaggatttcg
      721 cccgcggcgt cttcgattcc gtgctcgaca tgctctccgg ccgctacccc tccgacgagt
      781 tcgccgaatt gcgcccgcgc ctcacctggg accgcgtcac ccaccagctc acctcccgcc
      841 agggcgcgcg ctccgtcgcc gtcatcaacg gcggcaccat ccccgaccgc ggcttgttca
      901 ccgtctacct cgccggcgcc acccgcggcg cgcgcgtcgg cgaactcgac gaggagatgg
      961 tcttcgagcg ccgcgccggg gacaccatca tcctgggcgc caccacctgg cgcatcgacc
     1021 agatcaccca cgatcgcgtc aacgtcaccc ccgcccccgg ccagcccggc aagatgccct
     1081 tctggcacgg cgatagcgcc ggacgccccg ccgaattcgg ccgcgagatc ggcgagatga
     1141 cgcgcgaact gctcgccacg ccgcgcgccg ccgccttcac acgcctggtg gaacagcaca
     1201 gcctggatgc cggcgccgcc gagaacctgc tgcgctatct ggaggaccag accgctgcca
     1261 ccggccgcgt gccaagcgat cgcgacatcg tcatcgaacg ctgccgcgac gagctcggcg
     1321 attggcgcgt ctgcgtcctc actccgttcg gcagccgcgt acacgccccc tggtgcatgg
     1381 ccgtcaccgc caagctgcgc gccgaacgcg gcgtcgaagt ggagaccatg tggagcgacg
     1441 acggcttcgt gattcgtctg ccggaaagcg aagagccgct ggagaccgag tggctgctgc
     1501 cggcgcctgc agaactcaaa ga
//
LOCUS       DASVII010000506         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_634401,
            whole genome shotgun sequence.
ACCESSION   DASVII010000506 DASVII010000000
VERSION     DASVII010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634401"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF309_06575"
     CDS             complement(<1..498)
                     /locus_tag="VF309_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466286.1"
                     /translation="MSARIAALVLACAALPAIGAEADPVPTPEGWRKESFTFPLQFAP
                     SIPYEGQEHVRFTPSWTKFAEENGFSYVFLWDLKAKPVTPEDIEEYLETYFNGLMTNV
                     GKQRQLSDKETKAVTAVHPMASLPGWGQGYGVEVRTWNAFSKGEPLLLYGEIGQRECG
                     ERMQFF"
     gene            complement(505..771)
                     /locus_tag="VF309_06580"
     CDS             complement(505..771)
                     /locus_tag="VF309_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466287.1"
                     /translation="MKKADLEKLKGRKIAAGSGTTHDRFGKGAAEVLDKREQRERDRE
                     QGLVPFAVKLHGDLVRDLHAAAEKRGVGLNELTAELLRKGLAAK"
     gene            817..1233
                     /locus_tag="VF309_06585"
     CDS             817..1233
                     /locus_tag="VF309_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX7466288.1"
                     /translation="MKRDWRGIYDSLVVGRSGIVGKGLFSGTRIPARAKIGEFEGEVI
                     GLRESRRRAKGRAIVAIVELDRHALDATASPRGFRFINHSCDPNTYIRCTADRAEFYA
                     LRAIAKGEELTADYGESHHDGKLPCRCGAARCRGFI"
     gene            complement(1237..>1642)
                     /locus_tag="VF309_06590"
     CDS             complement(1237..>1642)
                     /locus_tag="VF309_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7466289.1"
                     /translation="LGICVFLLVFARVAWRSVASPPALPPGPRWMELAAKALQGALYL
                     LFFAVPLIAIAGAWLEGHPLTFLAGLQVAPLLPLAHDAGAQVASVHTWLGDALLWLAG
                     LHAVAAIFHHIFLKDEVLVSMLPRWTRLQRRA"
BASE COUNT          255 a          547 c          572 g          268 t
ORIGIN      
        1 gaagaactgc atgcgctcgc cgcactcgcg ctggccgatc tccccataga ggaggagcgg
       61 ctcgcccttg gagaaggcgt tccaggtgcg cacctcgacg ccgtagccct ggccccagcc
      121 cggcagcgac gccatcgggt gcaccgcggt gaccgccttg gtttccttgt ccgacaactg
      181 gcgctgcttg cccacgttgg tcatgagccc gttgaaatag gtctcgagat attcctcgat
      241 gtcctcgggc gtgaccggct tggccttcag gtcccacagg aagacgtagc tgaagccgtt
      301 ctcttccgcg aacttggtcc acgagggcgt gaagcgcacg tgttcctggc cctcgtaggg
      361 aatcgagggc gcgaactgca gcgggaatgt gaacgattcc ttgcgccagc cctccggcgt
      421 gggcacaggg tcggcctccg cgccgatcgc ggggagtgcc gcgcaggcga gcaccagcgc
      481 ggcgatgcgg gcgctcatcg agcgctattt ggccgcgagg cccttgcgca gcagctcggc
      541 ggtgagctcg ttcaggccca cgccgcgctt ctcggcggcc gcgtgcaggt cgcgcaccag
      601 gtcgccgtgg agcttcaccg cgaagggcac cagtccctgc tcgcggtcgc gctcgcgctg
      661 ctcgcgcttg tcgagcacct ccgcggcgcc ctttccgaat cgatcgtggg tggtgccgct
      721 tcctgcggcg atcttgcggc ccttcagctt ctcgaggtct gccttcttca tgattttcgt
      781 cttgtctcgt ggtgtcgctt ccgcgattat gacgaaatga aacgtgattg gcgcggcatt
      841 tacgattcgc tcgtggttgg caggagcggg atcgtcggca aggggctctt cagcgggacg
      901 cgcattccgg cgcgcgcgaa gatcggggag ttcgaagggg aagtgatcgg gctgcgggaa
      961 tcccggcggc gcgcgaaggg ccgcgccatc gtcgccatcg tggagctcga ccgccacgcg
     1021 ctcgacgcga ccgcgagccc gcgcggcttt cgcttcatca accactcctg cgaccccaac
     1081 acgtatatcc gctgcaccgc ggatcgcgcg gagttctatg cgctgcgcgc gatcgccaag
     1141 ggcgaggagc tcaccgccga ctacggagaa tcgcaccacg acggcaagct gccgtgccgg
     1201 tgcggcgcgg ccaggtgccg cgggttcatc tagccgctag gccctgcgct gcaggcgcgt
     1261 ccagcgcggc agcatcgaca ccagcacctc gtccttgagg aatatgtggt ggaagatcgc
     1321 ggccacggcg tgcaggcccg cgagccacag gagcgcgtcg ccgagccagg tgtgcacgct
     1381 cgccacttgt gccccggcgt cgtgcgcgag cggcagcagc ggggccacct gcaatccggc
     1441 gaggaacgtg aggggatggc cttccagcca cgcgccggcg attgcgatga ggggcacggc
     1501 gaagaaaagc aggtagagcg cgccctgcag ggccttggcg gcaagctcca tccagcgcgg
     1561 accggggggc agcgcgggtg gggacgccac gcttcgccac gccacgcgcg cgaagacgag
     1621 gaggaacacg cagatcccga gc
//
LOCUS       DASVII010000507         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_59718, whole
            genome shotgun sequence.
ACCESSION   DASVII010000507 DASVII010000000
VERSION     DASVII010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59718"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(175..369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            437..637
                     /gene="zapB"
                     /locus_tag="VF309_06595"
     CDS             437..637
                     /gene="zapB"
                     /locus_tag="VF309_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEX7466290.1"
                     /translation="MDPELDALEEKLAQLVQRLETLRDENRELRQQVATRTDENSRLG
                     EKLSAAQSRLAARLKQIPETET"
     gene            634..975
                     /locus_tag="VF309_06600"
     CDS             634..975
                     /locus_tag="VF309_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7466291.1"
                     /translation="MSGEPKARERGALTVSLLGREFRVACPDGEEKQLLASADYLNRK
                     LKEVRDTGKVTGNERIAIMAALNIAHEFVANQGNASSPSSIDGAAIRRRIVAMQETLD
                     SALAADQEKLF"
     gene            1002..1180
                     /gene="ssrS"
                     /locus_tag="VF309_06605"
     ncRNA           1002..1180
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF309_06605"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1183..1659
                     /locus_tag="VF309_06610"
     CDS             1183..1659
                     /locus_tag="VF309_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX7466292.1"
                     /translation="MAYWLMKSEPDEFSIDDLVKAPKQTTSWFGVRNYVARNFMRDTM
                     RIGDGVLFYHSSCAVPGIAGLAKVSSKPHPDESQFDRKSPYYDSKSKRDEPRWMLVDV
                     KLAKKTRVMPLEEMRTYPELAGMITLKRGNRLSITPVTGEEWSFIVARLAEHGPGG"
     gene            1660..2457
                     /locus_tag="VF309_06615"
     CDS             1660..2457
                     /locus_tag="VF309_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7466293.1"
                     /translation="MPLAELGAYLAIGALVGFFAGLLGVGGGLITVSALALMFTAHGF
                     APPYIMHLAVGTSLAAIVSGSWSSFRTHHRHGAVDWPIVRRVTPGLLVGVAAGAATSH
                     FVSTAFLKYFFLTVMFLITAQMVFNMRPKPSRELPGTAGLAAMGVFAGLISSLMGGGA
                     AAVGVPFLTWCNVSTHRAIGTVAAIGFPLSIAGTIAYAASGWNAAGLPPYSLGFVYVP
                     AAIGISVTSILVAPYGARLAHRLKGATLRRIFAIFLVLTGLKVAISV"
     gene            complement(2454..3584)
                     /locus_tag="VF309_06620"
     CDS             complement(2454..3584)
                     /locus_tag="VF309_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7466294.1"
                     /translation="MTPRLDPNALTRELLAFNTINPPGQERACAHLLGKRLEDAGFRV
                     AYHEFAEGRTSLIARIGGGQAKPPICFTGHIDTVPLGAAHWHHDAFAGETDGDRLYGR
                     GSTDMKSGIAAFMVAALELAPRLERTAGLTLVITAGEEIGCEGARFLADHELLDRAGA
                     IVVAEPTANYPYIGHKGLAWFEIETTGVTAHGSMPEVGDNAIVKMARVIGDLEGFRFP
                     VESHAVMGKPTLNIGTIRGGLNTNSVPDEARITVDTRTVPGIDHVHLCDSLAALLAPR
                     GAKVRKIVDTPPLYTDPANEWVQEVFEACAPLLDGRPVPRTITFSTDGSDLKRGFGGP
                     PAVVLGPGEPTLAHQTDEWCSMSRVGQSVDLFRSLMQRWCAA"
     gene            complement(3601..>3919)
                     /locus_tag="VF309_06625"
     CDS             complement(3601..>3919)
                     /locus_tag="VF309_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7466295.1"
                     /translation="ALYKAAADYALTRGIIIADTKFEFGIDGSGKLHLIDEALTPDSS
                     RFWPLATYREGTSPESFDKQYVRNWLDSIGFERKPPAPPMPEEVALKTSEKYQEALAR
                     IAG"
BASE COUNT          614 a         1271 c         1354 g          680 t
ORIGIN      
        1 gggaagttgc ggcgtcgaaa cggactgggc gtgcgcgccc aggcttgcaa ggccggcgag
       61 gcccgcgctg cgcaggaagt tcatggcgag aagtccttct tcgagcggcg agcgtccccg
      121 catgccgcag gttgcgggac ggcacgcgcc catctgcggg cgtgtccctc ccagggcttt
      181 gcttcgcgtc gggaccggtc tccgggctcg cgagaatcgc ggattcgccg ccttcccacg
      241 tccttgcggg cgcagtggca tgagggcgat tccagactcg cttaccgttg cgggggcagc
      301 gccggctttt caccggcttc ccgtgcatcc gttcgctgcc gaaatgatac cccaccccgg
      361 gcggaccgct cgaaacgact ttcagaatga agcgttatgc attgacgtga cttgggattt
      421 ccaatagggt ttattcatgg acccggaact cgacgccctc gaggagaagc tggcgcagct
      481 tgtgcagcgc ctcgagaccc tgcgcgacga gaaccgcgag ctgcgccagc aggtcgccac
      541 gcgcaccgac gagaactcgc gcctcggcga aaagctctcg gcggcgcagt cgcgcctcgc
      601 ggcgcgcctc aagcagattc cggagacgga gacgtgagcg gcgagccgaa ggcgcgcgag
      661 cgcggcgcgc tcaccgtgag cctgctggga cgcgaattcc gcgtcgcgtg ccccgacggc
      721 gaggaaaagc agctgctcgc ctccgccgat tacctcaatc gcaagctcaa ggaagtccgc
      781 gataccggca aggtgacggg caacgagcgc atcgcgatca tggccgcgtt gaacatcgcc
      841 cacgagttcg tggcgaacca ggggaacgct tcttcgccat cttcgattga cggggccgcg
      901 attcgtcgta gaatcgttgc aatgcaggaa acgcttgatt cggcgctcgc ggccgatcag
      961 gaaaagctct tctagaagcg gcgtatcgct cccggcaggc cggtttcctg cggtgttcgt
     1021 ccaggcttac atccttgagc cgataagctg gtagcttgga cgcttgccca gggcactatt
     1081 gtgcgcgtcc cacgtcggac gtacctgcgg tgcccggtga gcgaccgcct tggacctctg
     1141 ggttcgagag tgcggcctgg acgaacaccg cagggaatcg aaatggccta ctggttgatg
     1201 aagtccgagc ccgacgagtt ctccatcgac gatctcgtca aggcgcccaa gcaaaccacc
     1261 tcctggttcg gcgtgcgcaa ctacgtggcg cgcaatttca tgcgcgacac catgcgcatc
     1321 ggcgacggcg tgctcttcta ccattcgagc tgcgcggtgc cgggaatcgc gggcctcgcg
     1381 aaggtctcga gcaagcccca tcccgacgaa tcgcagttcg accggaagag cccgtactac
     1441 gattccaagt cgaagaggga cgagccgcgc tggatgctgg tggacgtgaa gctcgcgaag
     1501 aagacgcgcg tgatgccgct ggaagagatg cgcacgtacc cggagctcgc ggggatgatc
     1561 accttgaaac gcggcaaccg cctctcgatc acgcccgtga ccggcgagga atggagtttc
     1621 atcgtcgcca ggctggctga acatgggccc ggtggataga tgccgctagc ggagctgggc
     1681 gcgtacctcg cgatcggcgc gctcgtcggc ttcttcgcgg gcctcctcgg cgtcggcggc
     1741 ggcctcatca ccgtatccgc cctcgcgctc atgttcacgg cgcacggatt cgcgccgccc
     1801 tacatcatgc atctcgccgt gggcacctcg ctcgccgcga tcgtgtcggg ctcgtggtcg
     1861 agcttccgca cccatcaccg ccacggcgcc gtcgactggc cgatcgtgcg gcgcgtgacg
     1921 ccgggacttc tcgtgggcgt ggccgcgggc gccgcgacct cgcacttcgt gtcgaccgcc
     1981 ttcctcaagt acttcttcct caccgtcatg ttcctcatca cggcgcagat ggtgttcaac
     2041 atgcgcccga agccgtcgcg cgagcttccc ggaacggccg ggcttgccgc gatgggggtc
     2101 ttcgccggcc tgatctcgag tctcatgggc gggggcgcgg ccgcggtcgg agtgcccttc
     2161 ctcacgtggt gcaacgtgag cacccatcgc gccatcggca cggtcgcggc gatcggattt
     2221 ccgctgtcga tcgcgggaac gatcgcgtat gccgcatcgg gttggaacgc cgccgggctg
     2281 ccgccgtata gcctgggatt cgtctacgtg cccgccgcca tcggcatttc ggtgacgagc
     2341 atcctcgtcg cgccctacgg cgcgcgcctc gcgcaccggc tgaagggcgc gacgctgcgg
     2401 cgcatcttcg cgatattcct ggtccttacc gggctgaagg tcgcgatctc ggtctaggcg
     2461 gcgcaccagc gctgcatcaa cgagcgaaag agatcgaccg attggccgac gcggctcatc
     2521 gagcaccatt cgtcggtctg gtgcgcgagc gtgggctcgc ccggcccgag gacgacggcg
     2581 ggggggccgc cgaagccgcg cttcaagtcc gagccgtcgg tcgagaaggt gatcgttctc
     2641 ggaaccggtc gtccgtcgag caacggagcg caggcctcga agacttcctg cacccattcg
     2701 ttggcgggat cggtatagag cggcggcgtg tcgacgatct tgcgcacctt cgctccgcga
     2761 ggcgcgagga gggccgcaag cgaatcgcaa agatgcacgt ggtcgatgcc gggcacggtg
     2821 cgcgtgtcga ccgtgatgcg cgcctcgtcg ggcaccgagt tggtgttgag cccgccgcgg
     2881 atggtgccga tgttgagcgt gggcttgccc atcaccgcgt gggactcgac gggaaagcgg
     2941 aagccctcga ggtcgccgat gacgcgggcc atcttcacga tcgcgttgtc gccgacttcg
     3001 ggcatcgagc catgggcggt gacgccggtg gtctcgatct cgaaccacgc gaggcccttg
     3061 tggccgatgt agggatagtt cgccgtcggc tcggcgacca cgatcgcgcc ggcgcgatcg
     3121 aggagctcgt ggtcggcgag aaaccgcgcg ccctcgcatc cgatctcctc gcccgccgtg
     3181 atcacgagcg tgaggcccgc ggtgcgctcc agccgcggcg caagctcgag ggcggcgacc
     3241 atgaacgcgg cgatgccgct cttcatgtcg gtggagccgc ggccgtagag gcggtcgccg
     3301 tcggtctcgc cggcaaacgc gtcgtggtgc caatgggcgg cgcccagggg cacggtgtcg
     3361 atgtgccccg tgaagcagat cgggggcttc gcctgcccgc cgccgatccg cgcgatgagg
     3421 ctcgtgcgcc cctcggcgaa ctcgtgatag gcgacgcgaa agcccgcatc ctcgagccgc
     3481 ttgccgagta ggtgcgcgca tgcgcgctcc tgccccggag ggttgatcgt gttgaacgcg
     3541 agcagctcgc gcgtgagggc gttgggatcg aggcggggag tcatgttgcc ggcgaaggcg
     3601 ctagcccgcg atgcgcgcga gcgcttcctg gtatttctcg gaggtcttga gcgcgacttc
     3661 ctcgggcatc ggcggcgcgg gcggcttcct ctcgaagccg atggaatcga gccagttgcg
     3721 cacgtactgc ttgtcgaagc tctcggggct cgtgccctcg cggtaggtcg cgagcggcca
     3781 gaagcgcgac gaatccggcg tgagcgcctc gtcgatgagg tggagctttc cggagccgtc
     3841 gatgccgaat tcgaacttgg tgtcggcgat gatgatgccg cgagtgagcg cgtaatccgc
     3901 ggcggccttg tagagcgcg
//
LOCUS       DASVII010000508         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_598905,
            whole genome shotgun sequence.
ACCESSION   DASVII010000508 DASVII010000000
VERSION     DASVII010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598905"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF309_06630"
     CDS             <1..205
                     /locus_tag="VF309_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX7466296.1"
                     /translation="RDRPYKPGKTLSDSLAILGRMKENGHVDPDLFDIFVRDKVYLRY
                     AREFLDPEQIDQVDETRIPGYKP"
     gene            269..>1832
                     /locus_tag="VF309_06635"
     CDS             269..>1832
                     /locus_tag="VF309_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466297.1"
                     /translation="MTTRLPVGIFFALFTISGFAGLIYESIWSHYLKLFLGHAAYAQT
                     LVLAIFMGGMAIGSWLASRFTQRIGNLLLGYAFAELGIGLLAIAFHGTFLAATRWAFD
                     TAMPALGGTGIDLFKWSLASLLILPASILLGTTFPLMSAGIMRLYPESGGRSLSMLYF
                     TNSFGAAIGVLASGFYLIDHFGLPGTILTAGLLNVTLALVVWLIARRVAVVAPRAPAP
                     GASGPSAGRDITRAVLVLAFSTGAASFIYEISWIRMLSLGLGASTHSFEVMLSAFILA
                     MSLGAFWFRNRIARLRSDLGWLAGLLVAKAFFAVYAVWIYADVLDFIHWTFLAVSRTS
                     EGYVLFTGAGMLASMLVMFPAAFCAGMTLPLATHALTSRGVGESAIGKVYGANTAGCI
                     VGAAFATHVGMELAGLKGLTGIGALLDVAVGSLVLVAGVGREERRRSLALVAVVFAAG
                     ATAFFTTQLDLLRMSSGVYRNGSFANPSESKVAFYADGKTATIAVVDSGTLRSIKTNG
                     KTDASIQRDPAKG"
BASE COUNT          253 a          667 c          580 g          332 t
ORIGIN      
        1 gagggaccgc ccgtacaagc cgggcaagac gctgtcggac tcgctcgcga tcctcgggcg
       61 aatgaaggaa aacggccacg tcgatccgga tctcttcgac atcttcgtga gagacaaggt
      121 gtacctgcgc tacgcccgcg aattcctcga tcccgagcag atcgaccagg tggacgagac
      181 gcgcatcccc ggctacaagc cgtgagcgcg cggttctcgc gcgcgccgcg aagagccgca
      241 tcctgaagtc cgccttcccg ccgtccccat gacgacacgc ctgcccgtcg ggatcttctt
      301 cgccctcttc acgatctcgg gcttcgccgg gctcatctac gagtcgatct ggagccacta
      361 cctcaagctc ttcctcggcc acgcggccta cgcgcagacc ctcgtcctcg ccatcttcat
      421 gggcggcatg gcgatcggct cgtggctcgc gagccgcttc acgcagcgca tcggcaatct
      481 cctcctgggc tatgcgttcg cggagctcgg catcggcctt ctcgcgatcg ccttccacgg
      541 cacgttcctc gccgccacgc gctgggcatt cgacaccgcg atgcccgcgc tcggcggcac
      601 cggcatcgat ctcttcaaat ggagccttgc ctcgctcctc atcctgcccg cgtcgatcct
      661 gctcggcacg acctttccgc tgatgagcgc cggcatcatg cggctctacc ccgagtcggg
      721 agggcggtcc ctctcgatgc tgtacttcac caacagcttc ggcgcggcga tcggcgtcct
      781 cgcgagcggc ttctacctga tcgaccactt cgggcttccc gggacgatcc tcaccgcggg
      841 cctgctcaac gtgacgctcg cgctcgtcgt gtggctcatc gcgcggcgcg tggcggtcgt
      901 ggcgccgcgc gcccccgcgc caggcgcatc gggcccatcg gcgggccggg acatcacgcg
      961 cgcggtcctg gtgctcgcat tctccacggg cgcggcgtcc ttcatctacg agatcagctg
     1021 gatccgcatg ctgagcctgg gcctgggcgc ctccacgcac tcgttcgagg tgatgctttc
     1081 cgccttcatc ctcgcgatgt ccctgggcgc gttctggttc cgcaaccgca tcgcgcggct
     1141 gcgcagcgac ctcgggtggc tcgcgggact cctcgtggcc aaggcgttct tcgcggtcta
     1201 cgccgtgtgg atctacgccg acgtgctcga cttcatccac tggacgttcc tcgcggtgtc
     1261 gcgcacgagc gagggttacg tcctgttcac cggcgccgga atgctcgcct cgatgctcgt
     1321 catgttcccg gcggcgttct gcgcgggaat gacgctgccg ctggcgaccc atgcgctcac
     1381 ctcgcgcggc gtcggcgagt cggcgatcgg caaggtgtac ggcgccaaca ccgcgggctg
     1441 catcgttggc gccgcgttcg cgacgcacgt cggaatggag ctcgccggat tgaaggggct
     1501 caccggcatc ggcgcgctcc tcgacgtcgc ggtgggctcc ctcgtcctcg tggcgggcgt
     1561 cgggcgggaa gagcgcaggc gctccctggc gctcgtcgcg gtggtcttcg ccgcgggcgc
     1621 gacggcattc ttcacgacgc agctcgacct cctgcgcatg agctcgggcg tgtaccgcaa
     1681 cggctctttc gccaatccct cggagagcaa ggtcgcgttc tacgccgacg gcaagacggc
     1741 gaccatcgcg gtcgtcgaca gcgggaccct gcgaagcatc aagaccaacg gcaagaccga
     1801 tgcgagcatc cagcgcgatc ccgcgaaggg ca
//
LOCUS       DASVII010000509         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_24224, whole
            genome shotgun sequence.
ACCESSION   DASVII010000509 DASVII010000000
VERSION     DASVII010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24224"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VF309_06640"
     CDS             complement(<1..671)
                     /locus_tag="VF309_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7466298.1"
                     /translation="MDMNPERVDFRTEPSRYRHWKLAFDGPVATLSMDVAEDAGLRPG
                     YKLKLNSYDLGVDIELADALNRIRFEHPGVRAVIVTSAKDRVFCSGANIFMLGTSSHA
                     WKVNFCKFTNETRNGIEDSSHHSGLKFVAALNGTCAGGGYELALACDEIVLVDDRSSA
                     VSLPEVPLLGVLPGTGGLTRLTDKRRVRHDLADLFCTTAEGVRGQRALDWKLVDAIVK
                     PAQFV"
     gene            714..2288
                     /locus_tag="VF309_06645"
     CDS             714..2288
                     /locus_tag="VF309_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX7466299.1"
                     /translation="MSAPHPPGERFNFAQHLFELNRGRAGSAAYIDDNESMTYGELDE
                     RARRMAATLLAMGLRREERVLLLMHDNNDWPVSFLGALYAGVVPVAVNTLLAADDYAF
                     ILGHSRSRGALVSQALLPMLQKAMAQAPHEIGFVLVSRAESALPAGAVPLREAIAKHA
                     PLERAAATHPEDIAFWLYSSGSTGKPKGTLHTHANPYWTAELYGKAVLGITARDLCFS
                     AAKLFFAYGLGNALTFPLSVGARVLLMAERPTPDAVFKRWTTARPTIFFGAPTGYAGM
                     LASPNLPARKDVALRMCSSAGEALPKDIGERFTARYGCEIIDGIGSTEMLHIFISNRP
                     GDVAYGTTGKPVPGYDVELRGEDGRPVADGEIGDLFIRGPSAALMYWTQRAKSAETFQ
                     GAWTKSGDKYLRDAAGNYVYAGRSDDMLKVSGQYVSPFEVEATLVRHPAVLEAALIGV
                     PDDNGLTRSKAFVVLKDGRAGSPELEAELKAFVKEALAPYKYPRAIQFISELPKTATG
                     KIQRFKLREMEAARAR"
BASE COUNT          388 a          816 c          797 g          376 t
ORIGIN      
        1 tgcacgaact gcgcgggctt cacgatcgcg tccaccagct tccagtcgag cgcgcgctgg
       61 ccgcgcacgc cctcggccgt ggtgcagaag aggtcggcga ggtcatggcg cacgcgacgc
      121 ttgtcggtga gccgggtgag cccgccggtg cccggcaaca cgccgagcag tggcacttcg
      181 ggaagcgaca cggccgagga gcggtcgtcg accagcacaa tctcgtcgca ggcgagcgcg
      241 agctcgtagc cgccgcccgc gcaggtgccg ttcaacgcgg cgacgaactt gagtccggaa
      301 tgatgcgacg aatcctcgat gccgttgcgc gtctcgttgg tgaacttgca gaagttcacc
      361 ttccacgcgt ggctcgacgt cccgagcatg aagatgttgg ccccggagca gaagacgcgg
      421 tccttcgcgc tggtgacgat cacggcgcgc accccgggat gctcgaagcg gatgcggttc
      481 agcgcgtcgg cgagctcgat gtcgacgccg aggtcgtagg agttgagctt cagcttgtag
      541 cccgggcgca ggcccgcgtc ctcggcgacg tccatcgaga gcgtcgcgac ggggccgtcg
      601 aacgcgagct tccagtggcg ataccgcgaa ggctcggtgc ggaagtcgac gcgctcggga
      661 ttcatgtcca tggctttaca atactgcaaa tcaacgcctc cccggagtct cgaatgagcg
      721 cgccgcaccc gcccggcgaa cggttcaatt tcgcgcagca cctcttcgaa ttgaatcgcg
      781 ggcgcgcggg atccgccgcg tacatcgacg acaacgagtc gatgacctac ggcgagctcg
      841 acgagcgcgc gaggcgcatg gcggcaacgc tcctcgcgat ggggctgcgg cgcgaggagc
      901 gcgtgcttct cctcatgcac gacaacaacg attggccggt ctcgttcctc ggcgcgttgt
      961 acgcgggcgt cgtgcccgtc gccgtgaaca cgctcctcgc ggccgacgac tacgcgttca
     1021 tcctcggcca ttcgcgatcg cggggcgcgc tggtgtcgca ggcgctgctg ccgatgctgc
     1081 aaaaggcgat ggcgcaggcg ccccacgaga tcggcttcgt cctcgtgtcc cgcgccgaaa
     1141 gcgcattgcc ggcgggcgcg gtcccgttgc gcgaagcgat cgcgaagcac gctcccctcg
     1201 agcgcgccgc ggcgacgcat cccgaggaca tcgcattctg gctttactcg tcgggctcca
     1261 cgggcaagcc caagggcacg ctgcacaccc acgcgaatcc gtactggacc gcggagctct
     1321 acgggaaggc ggtgctcggc atcaccgcgc gcgacctttg cttctcggcg gcgaagctct
     1381 tcttcgccta cggcctgggc aacgcattga cctttccgct ctcggtcggc gcgcgcgtgc
     1441 tcctgatggc cgagcgtccc acccccgatg cggtcttcaa gcgctggacg acggcccggc
     1501 ccacgatctt cttcggcgcg ccgacgggct acgcgggaat gctcgcctcg ccgaaccttc
     1561 ccgcgcgcaa ggacgtggcg cttcgcatgt gctcgtcggc cggcgaggct ttgccgaagg
     1621 acatcggcga gcgcttcacc gcgcgctacg gctgcgagat catcgacggc atcggctcga
     1681 ccgagatgct gcacatcttc atctccaacc gtcccggcga cgtcgcctac ggcaccaccg
     1741 gcaagcccgt gccggggtac gacgtcgagc ttcgcggcga ggatggcagg cccgtcgccg
     1801 acggcgagat cggcgacctc ttcatccgcg gcccgagcgc ggcgctcatg tactggaccc
     1861 agcgcgccaa atccgcggag accttccagg gcgcgtggac aaagagcggc gacaagtacc
     1921 tgcgcgatgc ggccggcaac tacgtgtatg cgggacgcag cgacgacatg ctcaaggtga
     1981 gcggccaata cgtctctccc ttcgaagtcg aggcgacgct cgtgaggcat cccgcggtgc
     2041 tcgaggccgc cctgatcggc gtgcccgacg acaacgggct cacgcgctcg aaggccttcg
     2101 tcgtgctgaa ggacgggcgc gcgggctctc ccgagctcga ggcggagctg aaggcgttcg
     2161 tgaaggaggc gctcgcgccg tacaagtatc cgcgcgcgat ccagttcatc tccgagcttc
     2221 ccaagaccgc caccggaaag atccagcgct tcaagctgcg cgagatggaa gccgcgcgtg
     2281 cgcgctagcc cctcgggccg catcgacgcc ggtccgcagt tcgcggaaat ccaatgggac
     2341 tcgcgccccg ttcgcatcga gtacgaatgg gtcggtt
//
LOCUS       DASVII010000510         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_526934,
            whole genome shotgun sequence.
ACCESSION   DASVII010000510 DASVII010000000
VERSION     DASVII010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526934"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VF309_06650"
     CDS             <1..648
                     /locus_tag="VF309_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7466300.1"
                     /translation="IPVLLLKSGVQRAAIRVDEIIGNREVVVKTIGPQLSRLAGIAGA
                     TVLGNGQVVLILNPVQLVHRARGSAEVSVEAPKPQEAPFMAESKSGNPLVMVVDDSLT
                     VRKIMGRMLTREGYEVASAKDGVDALQQLQDMKPDLILLDVEMPRMDGFEFARNVRAD
                     EATKNIPIIMITSRTADKHRNHAIELGVNEYMGKPYQEEELLALIKRYTSEAALA"
     gene            complement(673..1482)
                     /locus_tag="VF309_06655"
     CDS             complement(673..1482)
                     /locus_tag="VF309_06655"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7466301.1"
                     /translation="MDLHSFFDAFIQGVVEGLTEFLPVSSTGHLIIVSDLLGLNDEKG
                     KVFDIVIQLGAILAICWEYRARFAKAFAGLASDPVQQRFAANLFVAFLPAAIVGLAFQ
                     KQIKAYLFNPVSVAMALIVGALAIFVIERWYARHGAPRVTSVDEMDWKDALKVGLAQC
                     FSLIPGTSRSGATIMGGMLFGLSRQAATEFSFFLAVPIMFAASGYQMVKYRALLSRDD
                     LATFAVGFIVSFAFALLAVKALIRYVARHDFKAFAWYRIALGVVVLGYFSR"
BASE COUNT          315 a          516 c          502 g          214 t
ORIGIN      
        1 atcccggtgc tgttgctcaa gagcggcgtg cagcgcgccg cgatccgcgt cgatgagatc
       61 atcggcaacc gcgaagtcgt ggtgaagacc atcggcccgc agctctcacg cctggcgggc
      121 atcgccgggg ccaccgtgct cggcaacggc caggtggtgc tgatcctgaa cccggtgcag
      181 ctcgtgcatc gcgcgcgggg ctcggccgag gtgagcgtcg aggcgccgaa gccgcaggaa
      241 gccccgttca tggccgagtc gaagtcgggc aatccgctgg tgatggtggt cgacgactcg
      301 ctcacggtgc gcaagatcat gggacgcatg ctgacgcgcg aaggctacga ggtcgcgagc
      361 gccaaggacg gcgtcgacgc cctgcagcag ctgcaggaca tgaagcccga cctcatcctg
      421 ctcgacgtcg agatgccgcg catggacggc ttcgaattcg cgcgcaacgt gcgcgccgac
      481 gaagcgacca agaacatccc gatcatcatg atcacctcgc gcaccgccga caagcaccgc
      541 aaccacgcga tcgagctcgg cgtgaacgag tacatgggca agccttacca ggaagaggag
      601 ctgctcgcgc tgatcaagcg atacacctcg gaagcggcac tcgcgtaggc cgagccggcc
      661 ggcgcttcgc gctcagcgcg agaagtaccc cagcaccacc acgccgagcg cgatgcgata
      721 ccaggcgaag gccttgaagt cgtgccgcgc cacgtagcgg atcagcgcct tcacggcgag
      781 gagcgcgaac gcgaagctca cgatgaatcc caccgcgaac gtcgcgaggt cgtcgcgcga
      841 cagcagggcg cggtacttca ccatctggta gccgctcgcc gcaaacatga tcggcaccgc
      901 gaggaagaac gagaactcgg tcgccgcctg ccgcgacagc ccgaacagca tccctcccat
      961 gatggtcgcc ccggagcgcg aagttccggg aatcaacgag aagcactgcg cgagccccac
     1021 cttgagcgcg tccttccagt ccatctcgtc cacgctcgtc acgcgcggcg cgccgtgcct
     1081 cgcgtaccag cgctcgatca cgaagatcgc gagggcgccg acgatgagcg ccatggcgac
     1141 cgagaccggg ttgaagagat aggccttgat ctgcttctgg aatgcgagcc cgacgatcgc
     1201 cgcgggaagg aacgcgacga acaggttcgc cgcgaagcgc tgctggacgg ggtcgctcgc
     1261 gagccccgcg aacgccttcg cgaagcgcgc gcgatattcc cagcagatgg cgaggatcgc
     1321 gcccagctgg atcacgatgt cgaagacctt gcccttctcg tcgttgaggc ccaggaggtc
     1381 cgacacgatg atgaggtggc ccgtcgagga aaccggcagg aactccgtca gcccctcgac
     1441 gaccccctgg atgaacgcat cgaagaacga atggagatcc atcgcgaagc ctcgtcgttg
     1501 aagcgactac tgcttcgcgt accagcgctc gatcacgaag atcgcga
//
LOCUS       DASVII010000511         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_491008,
            whole genome shotgun sequence.
ACCESSION   DASVII010000511 DASVII010000000
VERSION     DASVII010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491008"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1753)
                     /locus_tag="VF309_06660"
     CDS             complement(62..1753)
                     /locus_tag="VF309_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7466302.1"
                     /translation="MAQNSDSPRAPAWAATIWPLVTGLAVGFLVGRETGSRGSAGGGG
                     SEAAAEKGSDKAPAGTKMPAKIYKAESEFPSGWMKEADLASVTSVSFAGMTPGQKVTT
                     LQALNERDCECGCGMGKIAGCLKKDPNCPRSPNMAKLAIDMAKQGKGLGEILAAIDDK
                     QKPSAPAPGAAAAPGGSKKVVPFAHDLRRGPKAAKVTIVEFSDFQCPFCKRSEPTVKG
                     LLDKYGKDVALVWMNQPLPFHDHAMDAASAFQAAGRQNSEKAWKLHDKMYENNTALTR
                     GDIEKYAGEVGLDVGKLKKDWDDAKVKSEIAEDQKIATQVGASGTPTFFINGREIVGA
                     QESSAFEKIIDDEIKQADDLIKKGTPLKDVYTKRIESAALAPAPAAAPSAPAAPEGKQ
                     AIKLGDAPVKGPASAKVTLVAFSDFQCPFCSRAIPVLKELETAYPGKLRVAFKQMPLP
                     FHDKAHLAAEAALAANEQGKFWPYHDKLFANQQALDRPALEKYAEELGLDMGKFKKAL
                     DSGKFKDKVDSDAKEGAAVGATGTPTFFINGTKLVGAQPVDAFKTVIDQELKGKG"
     gene            complement(1848..>2102)
                     /locus_tag="VF309_06665"
     CDS             complement(1848..>2102)
                     /locus_tag="VF309_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466303.1"
                     /translation="GIIWTYMSVISRDMPRPARRMTMSRFVDQKTYRTFEEFEREELR
                     RDGYFESVDDMIDGMFAAGELDFDASTTRKSSAQDDDEEE"
BASE COUNT          285 a          732 c          677 g          408 t
ORIGIN      
        1 cggcggacgg cgggcgatgc gagcgagccc gaagggcgat gcgaggcgag cgatgccccg
       61 ttcagccctt acccttgagc tcctggtcga tcaccgtctt gaacgcgtcg acgggctgag
      121 cccccaccag cttggtcccg ttgatgaaga aggtgggggt gcccgtggcg ccgaccgcgg
      181 cgccctcctt cgcgtccgag tcgaccttgt ccttgaactt gccggagtcg agcgccttct
      241 tgaacttccc catgtcgagg ccgagctcct cggcgtactt ctcgagtgcg gggcgatcga
      301 gcgcctgctg gttggcgaac agcttgtcat ggtagggcca gaacttcccc tgctcgttgg
      361 cggccagggc cgcctcggcg gcgaggtgcg ccttgtcgtg gaacggcaac ggcatctgct
      421 tgaaggcgac gcgtagcttc cccgggtacg cggtctcgag ctccttgagg accgggatcg
      481 cccgcgagca gaagggacac tggaagtcgc tgaacgcgac cagcgtcacc ttcgcggagg
      541 ccgggccctt gaccggcgcg tcgccgagct tgatggcctg cttcccctcg ggcgcggccg
      601 gggcggacgg cgccgccgcg ggcgccggcg ccagcgccgc ggactcgatg cgcttcgtgt
      661 agacatcttt gagcggcgtc cccttcttga tgaggtcgtc cgcctgcttg atctcgtcgt
      721 cgatgatctt ctcgaaggcc gacgactcct gcgcgcccac gatctcgcgg ccattgatga
      781 agaaagtcgg ggtcccgctg gcgccgacct gggtcgcgat cttctgatcc tcggcgatct
      841 cgctcttcac cttcgcgtcg tcccagtcct tcttcagctt gccgacgtcg aggccgacct
      901 cgcccgcgta cttctcgatg tcgccacggg tgagggcggt gttgttctcg tacatcttgt
      961 cgtggagctt ccaggccttc tcggagttct ggcgacccgc cgcctggaac gccgacgccg
     1021 cgtccatcgc gtggtcgtga aacggcagcg gctggttcat ccagaccagc gccacgtcct
     1081 tgccgtactt atcgagcagc cctttgaccg tcggctccga gcgcttgcag aacgggcact
     1141 ggaagtcgct gaactcgacg atcgtgacct tggcggcctt ggggccgcgc cgcagatcgt
     1201 gcgcgaacgg aaccaccttc ttcgagccgc ccggggccgc cgccgcgccc ggggcgggcg
     1261 ccgacggctt ctgcttgtcg tcgatggccg ccaggatctc gccaagaccc ttgccctgct
     1321 tggccatgtc gatcgcgagc ttggccatgt tcgggctgcg cgggcagttc ggatccttct
     1381 tgaggcagcc ggcgatcttc cccatgccgc agccgcactc gcagtcgcgc tcgttgagcg
     1441 cctgcagagt cgtgaccttc tgccccgggg tcatccccgc gaagctgacg ctggtcaccg
     1501 aggcgagatc ggcttccttc atccacccgg atggaaactc gctctcggcc ttgtagatct
     1561 tcgccggcat cttggtgccg gcgggggcct tgtcgctccc cttctccgcg gcggcctcgc
     1621 taccaccgcc gccagccgac ccgcggctgc ccgtctctcg ccccaccagg aaaccgactg
     1681 cgagcccggt cacgagcggc cagatggtgg ccgcccatgc cggagcgcgc ggagaatcgc
     1741 tgttttgcgc catggcgggc ggtttaccac aggtgggacc ctgagtgggc cccgcaccac
     1801 ccgcaacggg cgcggccggg ccaagcccgt ccgttcccgg cgccttccta ctcctcttcg
     1861 tcgtcgtcct gcgcgctgct cttgcgcgtg gtagacgcgt cgaagtcgag ctcgccggcg
     1921 gcgaacatcc cgtcgatcat gtcgtcgaca gattcgaagt aaccgtcccg cctcagctcc
     1981 tcgcgctcga actcttcgaa ggtgcgatag gtcttctgat ctacgaagcg gctcatggtc
     2041 atcctccttg ctggtcgagg catgtcgcgt gaaatgaccg acatgtaggt ccagataata
     2101 cc
//
LOCUS       DASVII010000512         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_24357, whole
            genome shotgun sequence.
ACCESSION   DASVII010000512 DASVII010000000
VERSION     DASVII010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24357"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VF309_06670"
     CDS             complement(<1..406)
                     /locus_tag="VF309_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7466304.1"
                     /translation="MRALGRLALGLAAILAVALAALGATAAGDATPVPKLTAHVMDLT
                     GTLTATERESLDAKLRAFESTKGSQVVILLVPSIGAETIEEFAGRVTDEWKLGRKGVD
                     DGVLFVIAKQEHKLRIHTGRGVQGTLTDALSKR"
     gene            complement(417..1064)
                     /locus_tag="VF309_06675"
     CDS             complement(417..1064)
                     /locus_tag="VF309_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7466305.1"
                     /translation="MRKLLWALPLLAALTLSGCGYNDFQTRDEQVKAAWSEVLNQYQR
                     RADLIPNLVATVQGFAAQEKDVLIGVTQARARATGVTVTPETLNDPAAMQKFQQAQGE
                     LSGALGRLLAVAENYPQLKSDANFRDLQAQLEGTENRITTARNRFIEASRNYNVLARQ
                     FPTNLTAMMFGYKEKPSFTVEDEKAIARPPKVDFSPKSAVPAAPGSAPAAPATGK"
     gene            complement(1132..>1631)
                     /locus_tag="VF309_06680"
     CDS             complement(1132..>1631)
                     /locus_tag="VF309_06680"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7466306.1"
                     /translation="RKQAVFGVGAESAPWLFIGEGPGAEEDEMGEPFVGQAGKLLDSM
                     LAAIGIKRGREAYIANVVKCRPPGNRTPAAEEAGACAPFLDRQIELVKPKIIVALGKT
                     AAARLLDTEASLGSLRGKVHDYRGTRLVVTYHPAYLLRSLPEKAKAWEDLVFARSTLA
                     GAAAA"
BASE COUNT          222 a          553 c          572 g          284 t
ORIGIN      
        1 tgcgcttcga gagcgcgtcg gtcagcgtgc cctgcacgcc cctgccggtg tggatgcgca
       61 gcttgtgttc ctgcttggcg atcacgaaga gcactccgtc gtccacgccc ttgcggccca
      121 gtttccactc gtcggttacg cgcccagcga actcctcgat ggtctccgcg ccgatggatg
      181 gcaccagaag aatgacgacc tgcgagccct tcgtggactc gaacgcgcgc agcttcgcgt
      241 cgagggactc gcgctcggtg gccgtgaggg tcccggtcag gtccatcacg tgcgcggtga
      301 gcttcggcac cggcgtcgca tcgcccgctg ccgtcgcgcc gagagcggcg agcgccaccg
      361 cgaggatcgc ggccagcccg agagcgaggc ggcccagggc acgcatcacg gcgtcgctac
      421 ttgccggtcg cgggcgcagc gggcgcggag ccgggcgcgg cgggaaccgc gctcttgggc
      481 gagaagtcga ccttcggagg gcgtgcgatc gccttctcgt cctccacggt gaacgagggc
      541 ttttccttgt agccgaacat catcgcggtg aggttggtcg gaaactgccg cgcgagcacg
      601 ttgtagttgc gcgacgcctc gatgaagcga ttgcgcgcgg tcgtgatccg gttctcggtg
      661 ccctcgagct gcgcctgcag gtcgcggaag ttcgcatcgc tcttcagctg cggatagttt
      721 tccgcgaccg cgagaaggcg cccgagcgcc ccggagagct cgccctgcgc ctgctggaat
      781 ttctgcatcg ccgcgggatc gttgagggtc tcgggcgtga cggtcacccc ggtcgcccgg
      841 gcgcgcgctt gcgtcacgcc gatgagcacg tccttctcct gcgccgcaaa gccttgcacg
      901 gtggcgacga gatttgggat gagatccgcg cggcgctggt actggttcag cacctcggac
      961 caggcggcct tcacctgctc gtcgcgcgtc tggaagtcgt tgtagccgca acctgaaagc
     1021 gtcagcgccg cgagaagcgg cagggcccac agaagcttgc gcattgttct atctcccgga
     1081 tacggcccgc gatcgggcca gcgctgagga tgggggcggg accgggcgat ttcaagccgc
     1141 cgcggcgccg gcgagcgtgc ttctcgcgaa cacgaggtct tcccacgcct tggccttctc
     1201 cggaaggctg cgcaggaggt aggcgggatg gtaggtcacg acgaggcgcg tgcccctgta
     1261 gtcgtgcacc ttgccgcgga ggctcccgag gctcgcttcc gtgtcgagca ggcgggccgc
     1321 cgcggtcttg ccgagcgcga cgatgatctt cggtttcacg agctcgatct gccggtcgag
     1381 gaagggcgcg catgcgccgg cctcctccgc cgccggggtg cgattgcccg gcgggcggca
     1441 tttcacgacg ttcgcgatat aggcctcgcg gccgcgcttg atgccgatcg cggcgagcat
     1501 gctgtcgagc agcttcccgg cctggcccac gaacggttcg cccatctcgt cctcctcggc
     1561 gccggggcct tccccgatga agagccacgg ggccgattcg gcgcccacgc cgaacaccgc
     1621 ctgcttgcgc t
//
LOCUS       DASVII010000513         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_671204,
            whole genome shotgun sequence.
ACCESSION   DASVII010000513 DASVII010000000
VERSION     DASVII010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671204"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..570)
                     /gene="rpsH"
                     /locus_tag="VF309_06685"
     CDS             complement(172..570)
                     /gene="rpsH"
                     /locus_tag="VF309_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7466307.1"
                     /translation="MSMTDPIADFLSRIRNAIMARHAEVIAPASKLKHRIAELLKAEG
                     YIAAYDLREEDGVSNIVVRLRWADPRTNAITGLRRRSRPGQRLYVRHGTIPKIRSGLG
                     IAILSTSRGLMTDRAARKAGMGGELLCEVW"
     gene            complement(578..757)
                     /locus_tag="VF309_06690"
     CDS             complement(578..757)
                     /locus_tag="VF309_06690"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX7466308.1"
                     /translation="MAKTCDMHRPTPKWKVRHKNRCKQCGRSRAYLRKFELCRICFRS
                     LALRGDIPGVIKSSW"
     gene            complement(760..1323)
                     /gene="rplE"
                     /locus_tag="VF309_06695"
     CDS             complement(760..1323)
                     /gene="rplE"
                     /locus_tag="VF309_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7466309.1"
                     /translation="MDRVKRTVPARLRRVYDAEVRAKLISEFSLANPMEAPRLMKITI
                     NMGLGEAVTNPKVLDTAVENLGAITGQRPVVTRAKKSIAVFKLRAGQKIGAMVTLRRD
                     QMYEFLDRLVSYALPRVRDFKGVSPKSFDGRGNYTLGIREEIIFPEINYERLDKPKGM
                     NITFTTTARTDEQGRALLRHLGMPFRN"
BASE COUNT          239 a          551 c          559 g          342 t
ORIGIN      
        1 tcggcgcgca cgacctggag gttgctgcct tcgatcttga agctgatccc cagcggcatc
       61 accttggcca gctcgccctt cggccccttg acgccgaccg agccgccctt ctgggtgacg
      121 gcgaccccct tcgggagcgt aagaattttt cttccgatgc gcgacatgac gttaccaaac
      181 ctcgcagagg agctcgccgc ccatgcccgc cttgcgcgcc gcccgatcgg tcatcaggcc
      241 gcgcgacgtc gagaggatgg cgatcccgag cccggagcgg atcttcggga tcgtaccgtg
      301 acggacgtac aggcgctgcc cgggacgcga gcggcgccgc agcccggtga tggcgttggt
      361 gcgcggatcg gcccagcgca ggcggacgac gatgttgctg acaccatcct cctcgcgcag
      421 gtcgtaggcg gcgatgtagc cctcggcctt gagcagctcg gcgatgcgat gcttgagctt
      481 cgacgcgggg gcgatcacct ccgcgtggcg cgccatgatg gcgttgcgaa ttcgggacag
      541 gaagtcggcg atcggatcag tcattgacat gcgacaccta ccagctcgat ttgatgacgc
      601 cggggatatc accgcgcagc gccagggacc gaaagcagat ccggcaaagt tcaaacttcc
      661 ggaggtacgc ccgcgagcgc ccgcactgtt tgcatcggtt cttgtgacgg accttccatt
      721 tgggggtcgg tcggtgcata tcgcaggttt tggccatggt cagttcctga agggcattcc
      781 gaggtggcgc agcagcgcgc gcccctgctc gtcggtgcga gcggtggtgg tgaaggtgat
      841 gttcattccc ttgggcttat cgagacgctc gtagttgatc tccgggaaga tgatctcctc
      901 ccggatgccg agcgtgtagt ttccgcggcc gtcgaaggac ttgggagaga cccccttgaa
      961 gtcgcgcacg cgcgggagcg cgtacgagac cagccggtcg aggaactcgt acatctggtc
     1021 gcgccgcagg gtcaccatgg cgccgatctt ctggccggcg cgcagcttga acacggcgat
     1081 ggacttcttc gcgcgggtca cgacgggccg ctggccggtg atggcgccca ggttctcgac
     1141 ggcggtgtcg agcaccttgg ggttggtgac ggcttcgccg aggcccatgt tgatggtgat
     1201 cttcatgagc cgcggggcct ccatcgggtt cgccaggctg aactcgctga tgagcttggc
     1261 ccggacctcg gcgtcgtaga ccctgcgcag gcgcgccggc acggtccgct tgacccgatc
     1321 catcgcgatc tcctgcgacc ccgatgaccc cttgcccttg cccttgggga ccttgccgcc
     1381 cttggccttc gcctcggcgc gcgccgcctt cttggcgtcc gcctcggcct gttgctcggc
     1441 cgtcagcgtg ggcttggcgc ccttctgcga cttttcgttc ttatcttctt gctcggccat
     1501 tgagacgatt cctttgtctg ccgttgagcc ggtttacgcg gtctcgaaga ccgtgccgca
     1561 cttgccgcag atccggcgct tctgcgcgtg gtgctcgggg cccttgccgg tctctttgtt
     1621 gtcctgaacc ctggcgcgcc tcggagcgga gcacttcccg caccagagcg acacgttgga
     1681 cgactcgacc g
//
LOCUS       DASVII010000514         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_347587,
            whole genome shotgun sequence.
ACCESSION   DASVII010000514 DASVII010000000
VERSION     DASVII010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347587"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VF309_06700"
     CDS             complement(<1..1039)
                     /locus_tag="VF309_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7466310.1"
                     /translation="MAGRTWWFGLVAATALGLVGPSAAEETPEDAAEADGGAANVDAM
                     FEYLVAEIASQRGDTEGAVAVYERLARELKDPQIAKRAVETAIRARAFGPALESATLL
                     LELDPESALAREIMAALLANDPDVNKARDTLARILEKTPSRGPIIMQLAHLLGKFPDK
                     AAVLATTRDVTVRYPIVESHYAVGVAAILAGQVDLALTQADAALQMKPDWEPGAILKA
                     QALRKSAPAEVIPFYQKFVAANPGAQDVRMQLGRELAAERRLAEARDQFREAEKLAQD
                     DSHASYAIGLLSLQLEDYADAQVEFNKALKQGYREPTAIYLGLGQAAEGLQHFDEAIS
                     WYQRVDQGDWVR"
     gene            1212..2027
                     /gene="mutM"
                     /locus_tag="VF309_06705"
     CDS             1212..2027
                     /gene="mutM"
                     /locus_tag="VF309_06705"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX7466311.1"
                     /translation="MPELPEVETTRRGLLPRVAGRRIRSILVRNGSLRWPVPADIAKR
                     LSGETIRDIRRRAKYLLFDLRKGHLLVHLGMSGRLTLLPAGTPARKHDHVDIELEGGT
                     LLRFTDPRRFGAVLWLESPAEGHALLKDLGMEPLDAAFTGQALRARAKGRSVSIKQFL
                     MNGRIVTGVGNIYASEALFHAGIAPGRSAGGVSAARYDRLAEAVRRTLARAIEAGGST
                     LRDFASAEGRPGYFQQRHAVYKREGKPCRVCKTPIRALRQGQRSTFFCPQCQR"
BASE COUNT          310 a          708 c          716 g          333 t
ORIGIN      
        1 cgcgcaccca atcgccctgg tcgaccctct ggtaccagct gatcgcctcg tcgaaatgct
       61 gcaggccctc ggcggcctgg cccagcccga gatagatcgc ggtgggctcg cgatagccct
      121 gcttcagcgc cttgttgaac tcgacctgcg catcggcgta gtcctcgagc tgcagcgaca
      181 ggaggccgat ggcgtacgag gcgtgcgaat cgtcttgcgc aagcttctcc gcctcgcgga
      241 actggtcgcg cgcttcggcg agccttcgct cggcggcgag ctcgcgcccg agctgcatgc
      301 gaacgtcctg cgcgccggga ttggccgcga cgaacttctg gtagaaggga atcacttcgg
      361 cgggcgcgga cttgcgcagc gcctgcgcct tgagtatcgc gcccggctcc cagtcgggct
      421 tcatctgcag cgcggcgtcg gcttgcgtca gcgccaggtc cacctggccg gcgaggatcg
      481 cggccacgcc cacggcatag tgcgactcca cgatcggata tcgaacggtc acgtcgcgcg
      541 tcgtggcgag gaccgcggcc ttgtcgggaa attttccgag gagatgcgcg agctgcatga
      601 tgatcgggcc gcggctcggg gtcttctcga ggatgcgcgc gagcgtgtcg cgggccttgt
      661 tgacgtccgg atcgttggcg aggagcgccg ccatgatctc gcgggcaagc gccgattcgg
      721 gatcgagctc gaggagaagg gtcgcgcttt cgagcgccgg gccgaaggcg cgcgcgcgga
      781 tcgcggtctc gacggcgcgc ttggcgatct gcgggtcctt cagctcccgg gcgaggcgct
      841 cgtagacggc aacggcaccc tcggtgtcgc cgcgctgcga ggcgatctcg gcgacgaggt
      901 actcgaacat cgcatcgacg ttcgccgcgc cgccatcggc ctccgccgcg tcctccgggg
      961 tttcttcggc cgccgagggg ccgaccaagc ccagggccgt cgccgcgacc aatccaaacc
     1021 accaggttct gcccgccatt tcgcccccat gcgcgccgag aagcggcgcc cagacagacc
     1081 tatcatagaa cgattcgagc tccggctacg gccccgcaag cgtttcattc gtcgcggcgc
     1141 gcggcggcgg gaaagcgggg gttggcacgt ataatctccc cgaaaaatcc acactccatt
     1201 gtgactacgc gatgcccgag ctcccggaag tcgaaacgac gcgtcgcgga ctgttgccac
     1261 gcgtggcggg ccggcgcatt cgctcgattc tcgtgcgaaa cgggagcttg cgctggccgg
     1321 tgcccgcgga tatcgcgaag cggctgtcgg gcgagacgat tcgcgacatc cggcgccgcg
     1381 ccaagtacct cctcttcgac ctgcgcaagg gccatctcct cgtccatctc ggcatgtcgg
     1441 ggcgcctcac gctcctgccc gccgggacgc cggcgcgaaa gcacgatcac gtcgacatcg
     1501 agctcgaggg tggaaccctg ctacggttca ccgatccgcg gcgtttcggc gccgtgctgt
     1561 ggctcgagtc gccggccgag ggccatgcgc tgctcaagga tctcgggatg gagcctctcg
     1621 acgccgcgtt tacgggacag gcattgcgcg cgcgcgcaaa ggggcgcagc gtgtcgatca
     1681 agcagttcct catgaacggc cgaatcgtga cgggagtggg caacatctac gcgagcgagg
     1741 cgcttttcca cgcgggcatc gctcccggcc gaagcgccgg cggggtctcc gcggcgcgct
     1801 acgatcggct cgccgaggcc gtgcggcgca cgctcgcgcg cgcgatcgag gcgggcggga
     1861 gcacgcttcg cgatttcgcc tcggccgagg ggcggccggg ctactttcag cagcgccatg
     1921 ccgtgtacaa gcgggaaggc aagccgtgcc gcgtttgcaa gacgccgatc cgagcgctgc
     1981 gccagggaca gcgctcgacc ttcttctgcc cgcaatgcca gagatgaccg cacccggcga
     2041 gcggcgcggg ccttcgtcgc tcgaggc
//
LOCUS       DASVII010000515         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_131814,
            whole genome shotgun sequence.
ACCESSION   DASVII010000515 DASVII010000000
VERSION     DASVII010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131814"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF309_06710"
     CDS             complement(<1..284)
                     /locus_tag="VF309_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466312.1"
                     /translation="MAAASDVIGGARPAAEKQPSALSTIFFTEMWERFSYYGMRALLV
                     LYLVNSLGWERPHALELYGIYTGLVYLSPLLGGYLADRYLGFRKAVVIGGL"
     gene            complement(286..>1669)
                     /locus_tag="VF309_06715"
     CDS             complement(286..>1669)
                     /locus_tag="VF309_06715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX7466313.1"
                     /translation="AHTLDSKGEAIVAAFGLSQGNAGSVYRILANSDMPWPTVKLATG
                     REVKLDQSGYTDAREAANRDDRKKVFDAFFGKWKEFESTMGTTLYGAMKEDTVYTKVR
                     KYPDSLARSLDANNLPRSVYEALIKSANANLPTLHRYFRLRAKMLGVAEMRYYDIYPP
                     LVAGGREYPIDEGTRMMLEAVKPLGDAYVAAMKKGLDERWMDVYPRPRKLSGAHMAGE
                     AYDVHPLLLINYTNNYESVSTIAHEWGHAMHTYLSNKGQPYATSQYATFVAEIASTLN
                     EALLLDYALKNARTDEERLLYLGSALEGLRATFFRQAMFGEFEERTHALVDKGEPLTG
                     AEYTKIYADILRRYHGEAQGVVKIDDLYTVEWAYIPHFYNSFYVFQYATSIAASSLLA
                     DEILAGKPGARERYLKLISSGSADYPYNLVKAAGVDLATAAPYDAVAKRMDRIMDEME
                     TILLRRKKSY"
BASE COUNT          257 a          541 c          558 g          313 t
ORIGIN      
        1 aggccgccga tcaccaccgc cttgcggaag cccaggtagc ggtccgcgag atagccgccg
       61 aggagcggcg agaggtacac gagcccggtg tagatcccgt agagctcgag cgcgtggggg
      121 cgctcccatc cgagcgagtt gacgagatac agcaccagga gcgcgcgcat cccgtagtac
      181 gagaagcgct cccacatctc cgtgaagaag atggtggaga gggcgctcgg ctgcttctcc
      241 gccgccggcc tcgcgccgcc gatcacgtcg ctcgccgccg ccatgtcagt aggacttctt
      301 gcgccgcagg aggatcgttt ccatctcgtc catgatgcgg tccatccgct tcgccaccgc
      361 gtcgtagggc gccgcggtcg cgaggtcgac gcccgccgct ttcacgaggt tgtacggata
      421 gtccgcgctt cccgacgaga tgagcttgag gtagcgctcc cgggcgccgg gcttgcccgc
      481 gaggatctcg tcggccagga gcgagctcgc ggcgatcgag gtcgcgtact ggaacacgta
      541 gaacgagttg tagaagtgcg ggatgtacgc ccattcgacg gtgtacaggt cgtcgatctt
      601 caccacgccc tgcgcctcgc cgtggtagcg gcgcaggatg tcggcgtaga tcttcgtgta
      661 ctcggcgccg gtgagcggct cgcccttgtc gacgagcgcg tgggtccgtt cctcgaattc
      721 gccgaacatc gcctggcgga agaacgttgc gcgcaatccc tcgagcgccg agccgagata
      781 gagcaggcgc tcttcgtcgg tcctcgcgtt cttcagcgcg tagtcgagca gcagcgcctc
      841 gttcagcgtc gaggcgattt ccgccacgaa ggtcgcgtat tgcgaggtcg cgtacggctg
      901 ccccttgttc gacaggtacg tgtgcatcgc gtggccccac tcgtgcgcga tggtcgaaac
      961 cgactcgtag ttgttggtgt agttgatgag caggagcggg tgcacgtcgt aggcctcgcc
     1021 cgccatgtgc gcgcccgaga gcttgcgcgg gcgcggatac acgtccatcc aacgctcgtc
     1081 gagtcccttc ttcatcgccg ccacgtaggc gtccccgagg ggcttcaccg cctcgagcat
     1141 catgcgggtg ccctcgtcga tcggatactc gcgccctccg gcgaccagcg gcggatagat
     1201 gtcgtagtag cgcatctccg cgacgcccag catcttggcg cgcagcctga agtagcggtg
     1261 cagcgtcggc aggttcgcat tggcgctctt gatgagcgct tcgtacaccg agcgcggcag
     1321 gttgttcgcg tcgagcgagc gcgccaggga atcggggtac ttgcgcacct tcgtgtagac
     1381 cgtgtcctcc ttcatcgcgc cgtagagcgt cgtgcccatc gtggactcga actccttcca
     1441 cttgccgaag aaggcgtcga acactttctt gcggtcgtca cggttcgccg cttcgcgcgc
     1501 gtcggtgtac cccgactggt cgagcttcac ttcgcggccc gtggcgagct tcaccgtggg
     1561 ccagggcatg tccgagttgg cgaggatgcg atacacggac cccgcgttgc cctgcgaaag
     1621 tccgaaggcg gcgacgatcg cctcgccctt cgagtcgagg gtgtgggcc
//
LOCUS       DASVII010000516         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_707147,
            whole genome shotgun sequence.
ACCESSION   DASVII010000516 DASVII010000000
VERSION     DASVII010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707147"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1646
                     /locus_tag="VF309_06720"
     CDS             <1..>1646
                     /locus_tag="VF309_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7466314.1"
                     /translation="PIGSYETRTPVGVSASPDHTTRKQAEEALLKAGALQNAIFNSAN
                     FSSIATDAKGVIQIFNVGAERMLGYTAAEVMNKITPADISDPQEVIARAKALSAELGT
                     PITPGFEALVFKASRGIEDIYELTYIRKDGSRFGAVVSVTALRDEQDAIIGYLLIGTD
                     NTARQQVEEERKKLDQRLRDQHFYTRSLIESNIDALMATDPRGIITDVNKQTEALTGC
                     TRDELIGAPFKNYFTDSSRAEAGINRVLAEGKVTNYELTARARDGKQTTVSYNATTFH
                     DRDRRLQGVFAAARDVTELKRYEQTLHRKNVELESASRMKSEFLANMSHELRTPLNAI
                     IGFSEVLRDGLMGDMSDKQRGFIGDIFSSGNHLLSLINDILDLSKVEAGTMMLDLESV
                     SVSSLFVNSLSIIREKAAARHISLGIEADEELGSFQGDARKVKQIAYNLLSNAVKFTV
                     EGGKVTLRASRVPRADVGKLAGSWPGRTFPLADNEFTEFLKIGVGDSGIGISAEGMEQ
                     LFKPFSQIDSGLARKFEGTGLGLAMVKLLAELHGGSVAVE"
BASE COUNT          338 a          516 c          537 g          255 t
ORIGIN      
        1 tcctattgga agctacgaga cgaggacgcc ggtcggcgtc tccgcgagtc ccgaccacac
       61 cacgcgcaag caagcggaag aggcgctgct caaggcgggg gccttgcaga acgcgatttt
      121 caacagtgcc aacttttcga gcatcgccac cgacgcgaag ggcgtcatcc agatcttcaa
      181 cgtcggcgcc gagcgcatgc tgggttacac ggccgccgaa gtgatgaaca agatcacgcc
      241 ggccgacatc tccgatccgc aggaagtgat cgcgcgcgcc aaagcgctaa gcgccgagct
      301 cggaaccccg attacgccag gctttgaggc attggtgttc aaggcatcgc gtgggatcga
      361 ggacatctac gagctgacct acatccgcaa ggatgggagc cggttcggcg cggtggtgtc
      421 cgtaacggcc ctgcgcgacg agcaggacgc catcatcggc tatctgttga ttggcaccga
      481 caacaccgcg cgccagcagg tcgaagagga gcgaaagaag ctcgaccagc ggctgcgcga
      541 ccagcacttc tacacgcgct cgctcatcga atccaacatc gatgcgctga tggccaccga
      601 tccgcgtggg atcatcaccg acgtcaacaa gcagaccgag gccttgaccg ggtgcacgcg
      661 ggacgagctg atcggggcgc ctttcaagaa ctacttcacc gattcgagcc gggcggaggc
      721 gggcatcaac cgggtgctgg ccgagggcaa ggtcaccaac tacgagctca ccgcccgggc
      781 ccgggacggc aagcagacca ccgtgtcgta caacgcgacg acattccacg accgcgaccg
      841 gcggttacag ggcgtgttcg ccgccgcgcg cgacgtgacg gagctgaagc gttacgagca
      901 gacgctgcac cggaagaacg tggagctgga atccgccagc cggatgaaat ccgaatttct
      961 cgcgaacatg tcgcacgagc tccggacgcc gctgaacgcg atcatcggct tctccgaagt
     1021 gctgcgggac ggcctgatgg gcgacatgag cgacaagcag cgggggttca tcggcgacat
     1081 cttcagcagc ggcaaccatc ttctgtcgct catcaacgac atcctcgacc tgtccaaggt
     1141 ggaagccggc acgatgatgc tggacctgga gtcggtgtcg gtgtcgtccc tgttcgtcaa
     1201 cagcctctcc atcatccgag agaaggccgc ggcccggcac atcagcctgg gcatcgaggc
     1261 ggacgaagag ctgggttcct ttcagggcga cgcgcgcaag gtcaagcaga tcgcctacaa
     1321 cctgctctcc aacgcggtca aattcaccgt cgagggcggg aaggtgaccc tgcgcgccag
     1381 ccgcgtcccg cgcgccgacg ttggcaagct ggcgggctcc tggccgggcc ggacctttcc
     1441 gttggccgat aacgagttca cggagtttct caagatcggt gtcggcgaca gcggcatcgg
     1501 catctcggcg gaaggaatgg agcagctctt caagcccttc agccagatcg acagcggcct
     1561 ggcgcggaaa ttcgagggga ccgggctggg gctggccatg gtcaagctcc tggccgagct
     1621 gcacggcggc tcggtggcgg tggaga
//
LOCUS       DASVII010000517         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_275649,
            whole genome shotgun sequence.
ACCESSION   DASVII010000517 DASVII010000000
VERSION     DASVII010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275649"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF309_06725"
     CDS             complement(<1..224)
                     /locus_tag="VF309_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7466315.1"
                     /translation="MPTRRVAVLVGSLRKDSFTRRVAQALQQLQPAGLHLEIVEIRDL
                     PLYDQDAEASPPAPWAAFRDSMRASDAVLFL"
     gene            316..1131
                     /gene="nudC"
                     /locus_tag="VF309_06730"
     CDS             316..1131
                     /gene="nudC"
                     /locus_tag="VF309_06730"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX7466316.1"
                     /translation="MIVTPASFTSLLAPMAHDRPRTFVFRGNELLLREADLGLPDPSV
                     VKALDIPPAHILPVGIHDKEYCAAAWIAHDAKPPEGCAFKGLRSLFGRVGDETLAVAG
                     RAFQVADWARSHQFCGVCGRPMTKIDGERAMRCSCGHTAYPRIAPAMMVLVKKADAIL
                     LARNVAVPAGGRMSALAGFLDPGESAEDAVHREVHEEVGLFVKDLRYFASQSWPFPGS
                     LMIAFTAEYSGGEIKVDPTEIAEARWFGPGDKLPELSPPQSISRALIEANLPR"
     gene            complement(1152..>1630)
                     /locus_tag="VF309_06735"
     CDS             complement(1152..>1630)
                     /locus_tag="VF309_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7466317.1"
                     /translation="QRAATISFSAAYVEIGGTYLVRADSPLRRIDEFDRKELRVAVAL
                     GSGYDLYLSRTLKNAELVRTANSQLAVDRFLGDRLDAVAGVRELLTRNAKSRPGLRVI
                     DENFMVIRPAAAVPKGHDAAAAYLGGFIEDAKASGFVADAVKRSGSTDATVAPAAK"
BASE COUNT          246 a          547 c          579 g          258 t
ORIGIN      
        1 aggaagagca cggcgtcgct cgcgcgcatg ctgtcgcgaa aggcggccca cggggccggc
       61 ggactcgcct cggcatcctg gtcgtagagc ggcaggtcgc ggatctccac gatctcgagg
      121 tgcaggccgg cgggctgcag ctgctgcagc gcctgcgcga cgcggcgcgt gaacgagtcc
      181 ttcctgaggc ttccgacgag cacggcaacg cggcgggtgg gcatgtatgg gctcctggat
      241 gtcgaggaag gcattctgaa tctttttcga ggatcgcgcg tcccgttata agatcgcggc
      301 caaggagcat ttcccgtgat cgtcacgccc gcgtccttca cctcgctgct cgcgccgatg
      361 gcgcacgatc gcccgcgcac gttcgtgttc cgcggcaacg agctgctgct gcgcgaagcg
      421 gacctcggcc ttccggatcc gtcggtcgtg aaggcgctcg acatcccgcc ggcgcacatc
      481 ctgcccgtcg ggattcacga caaggagtat tgcgcggcgg catggatcgc ccacgatgcg
      541 aaacctcccg agggctgcgc gttcaagggg ctgaggtcgc tcttcggacg cgtcggcgac
      601 gagaccctcg ccgtcgccgg gcgcgccttc caggtcgccg actgggcgcg cagccaccag
      661 ttctgcgggg tatgcggccg gcccatgacg aagatcgacg gcgagcgcgc gatgcgctgc
      721 tcgtgcggcc atacggccta tccgcgaatc gcccccgcca tgatggtgct cgtgaagaag
      781 gcggacgcga tcctcctcgc gcgcaacgtc gcggtgcccg ccggcgggcg catgagcgcg
      841 ctcgcgggat tcctcgatcc cggcgaatcg gccgaggacg ccgtgcaccg cgaggtgcac
      901 gaggaagtgg gcctcttcgt gaaggacctt cgctacttcg cgagccagtc gtggccgttt
      961 ccgggatcgt tgatgatcgc cttcaccgcg gagtactccg gcggcgagat caaggtcgac
     1021 cccacggaga tcgccgaggc gcgctggttc gggcccggcg acaagctgcc cgagctctcg
     1081 ccgccgcagt cgatctcgcg cgcgctgatc gaggcgaacc tgccccggtg aatgccttgc
     1141 gggaaggccg gctatttcgc ggcgggagcg acggtggcgt ccgtcgagcc gctgcgcttc
     1201 acggcgtcgg cgacgaatcc cgaggccttg gcgtcctcga tgaacccgcc gagatacgct
     1261 gccgcggcgt cgtgcccctt cgggacggcg gcggccgggc gaatgaccat gaagttctcg
     1321 tcgatcacgc gcaggcccgg acgcgacttg gcgttgcgcg tgagaagttc gcgcaccccg
     1381 gccacggcat cgagcctgtc cccgaggaag cgatcgacgg ccagctgcga attggcggtg
     1441 cgcacgagtt cggcattctt gagcgtgcgc gacaggtaga ggtcgtagcc cgagccgagg
     1501 gccacggcga cgcggagctc cttccggtcg aactcgtcga tcctgcgcaa cggcgaatcg
     1561 gcgcgcacga gataggtgcc gccgatctcg acgtaggccg cggagaacga gatcgtcgcc
     1621 gcgcgctgcg
//
LOCUS       DASVII010000518         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_419549,
            whole genome shotgun sequence.
ACCESSION   DASVII010000518 DASVII010000000
VERSION     DASVII010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419549"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="secB"
                     /locus_tag="VF309_06740"
     CDS             complement(<1..485)
                     /gene="secB"
                     /locus_tag="VF309_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX7466318.1"
                     /translation="MSEAPQNNQPVFSIEKIFVKDLSLEIPNAPQVFLEREAPAVDIQ
                     LHHNSTTIEDGVYQTTLTVTVTAKVGEKTLFLVEAAQAGIFVARNIPASELEAVLGIA
                     CPNILFPYVREVISDTVTRAGFPPVILSPVNFEAVYQQQRAQQPAIETPVQIITPGTS
                     H"
     gene            complement(528..788)
                     /gene="grxC"
                     /locus_tag="VF309_06745"
     CDS             complement(528..788)
                     /gene="grxC"
                     /locus_tag="VF309_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX7466319.1"
                     /translation="MAKVTMYSTGICPFCLMAERLLRSKGVAEIEKVRVDLDPPRRQE
                     MMERTGRRTVPQIYVGEKHVGGYDDLAALDRAGGLDALLAAP"
     gene            complement(793..1206)
                     /locus_tag="VF309_06750"
     CDS             complement(793..1206)
                     /locus_tag="VF309_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7466320.1"
                     /translation="MGEFIMNNIALIAIFLASGAMLAWPEISRLTGGSNEIGTLDATR
                     LMNQGTTLILDVRDKAEYADGHLPRARHIPLEELTKRIDEIGKYKEKPVLVTGKAGPR
                     AGAAARVLKQAGFTTVYQLRGGLGAWREASLPLEK"
     gene            complement(1251..1568)
                     /locus_tag="VF309_06755"
     CDS             complement(1251..1568)
                     /locus_tag="VF309_06755"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX7466321.1"
                     /translation="MSIWIVIAAVALATFLLRASFIVFADPHRFPHAFRQALTFVPPA
                     VLAAIVAPGLLMPQGVFDPTLANPRWIAGLVALAVAARMRNPLAPVAAGLPALWLLQW
                     AMG"
BASE COUNT          274 a          554 c          589 g          281 t
ORIGIN      
        1 cagtgcgacg tgcccggcgt gatgatctgc accggcgtct cgatcgcggg ctgctgggcg
       61 cgctgctgct ggtagaccgc ctcgaagttg acggggctca ggatgaccgg cgggaagccc
      121 gcgcgcgtca cggtgtccga gatcacttcg cgcacgtacg ggaagaggat attcgggcag
      181 gcgatgccga gcaccgcctc gagctcgctc gccggaatgt tgcgcgcgac gaagatgccc
      241 gcctgcgccg cttcgacgag gaacagggtc ttctcgccga ccttcgccgt gaccgtcacc
      301 gtgagcgtcg tctggtaaac gccgtcttcg atggtggtcg aattgtggtg cagctggatg
      361 tcgaccgccg gcgcctcgcg ctcgaggaac acctgcggcg cgttggggat ctcgagggac
      421 aggtccttga cgaagatctt ttcgatcgag aagacgggtt ggttgttctg cggggcttcg
      481 ctcatgaaac ttccttcggg aatgaatcgg tgatcgggaa acgcttgtca gggcgccgcg
      541 aggagggcat cgagcccccc ggcgcggtcg agcgcggcga gatcgtcgta gccgccgacg
      601 tgcttctcgc ccacgtagat ctgcggcacc gtgcgccgtc cggtgcgttc catcatttcc
      661 tggcgacgcg gcggatcgag gtcgacgcgg accttctcga tctcggcgac gcctttcgac
      721 ctcagcaaac gctcggccat caggcagaac gggcaaatgc cggtcgagta catcgtcact
      781 ttcgccatcg cgtcacttct ccagcggcag gcttgcttcg cgccaggcgc cgagcccgcc
      841 cctcaactgg tacacggtcg tgaagcccgc ctgcttcaga actcgcgccg cggctccggc
      901 gcgcggcccg gccttgcccg tgacgagcac gggcttttcc ttgtacttgc cgatctcgtc
      961 gattctcttc gtgagctctt ccagcgggat atgccgcgcg cggggcaagt gaccgtccgc
     1021 gtactcggcc ttgtcgcgca cgtcgaggat gagcgtcgtc ccctggttca tgagccgggt
     1081 cgcgtcgagc gtgccgatct cgttcgagcc gccggtaaga cgcgagatct cgggccaggc
     1141 gagcatcgcc ccgctcgcga ggaagatcgc gatcagcgcg atgttgttca tgatgaattc
     1201 gcccacgttg ctttcccgag ggcgcgccgg ccccgcaaaa agggacaatt ttaccccatc
     1261 gcccactgca agagccacag cgcgggcaag ccggccgcga ccggcgcgag gggattgcgc
     1321 atgcgggcgg ccaccgcgag cgccacgagc ccggcaatcc agcgcggatt ggcgagggtg
     1381 gggtcgaaca cgccctgtgg catgaggagt cccggcgcca cgatcgcggc gagcaccgcg
     1441 ggcggcacga aggtgagcgc ctggcggaag gcgtggggaa accggtgcgg atcggcgaag
     1501 acgatgaagg acgcgcgcag gagaaaggtc gcgagcgcca cggcggcgat gacgatccag
     1561 atgctcaccg gggcctcctt gcgagctcga tcgccatccc cgccgcgatg cccgcgatcg
     1621 acgcgatgac cagcgccagg cggtacggaa gccccgcggc gacaagcgct attcccgtgg
     1681 cgacggtcgc ggccgcga
//
LOCUS       DASVII010000519         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_275708,
            whole genome shotgun sequence.
ACCESSION   DASVII010000519 DASVII010000000
VERSION     DASVII010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275708"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..791)
                     /locus_tag="VF309_06760"
     CDS             complement(3..791)
                     /locus_tag="VF309_06760"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HEX7466322.1"
                     /translation="MRSPVYAPLEGARERLPAERWPTHDELTQAAGGIVTSRGVPLRF
                     VNPRGHTDRERRYYELRIAESGEVETRLENWHDLFNALVWITFPKAKAAINAQHAAIL
                     EERGEDEARRRSPERDALTLFDEGGVIVASSSDALSRLIPAFEWKDLFWHRRAEVEAS
                     MRFFAFGHALYEQALEPYLGMVAKTVFIDARPSFFSLPRDARLARADELVAAHFSDRA
                     RFPSPRSMAPMPVLGIPGWHPDTAIEAFYDDAEHFRSKGVRPSR"
     gene            complement(825..1862)
                     /gene="pyrC"
                     /locus_tag="VF309_06765"
     CDS             complement(825..1862)
                     /gene="pyrC"
                     /locus_tag="VF309_06765"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7466323.1"
                     /translation="MAESITITRPDDWHAHFRDGDLMESVVGATARQFARAIVMPNLK
                     PPVVSVKDAKAYRERILAALPKGAGFEPLMTLYLTDETPASEIEKARAAGFIKAVKYY
                     PAGATTNSASGVTDLRKCDAALEAMQAAGMPLLMHGEVTDPEVDVFDREVVFLERTLA
                     PLAKRFPKLKIVLEHITTHEAAAFVERAPETVAATVTVHHLLLNRNAMFSGGLRPHHY
                     CLPVLKREPHRRALVKAAMSGNPRFFLGSDSAPHTRRSKETDCGHAGLYTAHAALELY
                     AEAFHAAGALDKLEAFASHFGADFYGLPRNKGKVTLERVEWQVPDEYPFGAETVVPLR
                     AGQAIAWRIAG"
     gene            complement(2003..2902)
                     /gene="rsmI"
                     /locus_tag="VF309_06770"
     CDS             complement(2003..2902)
                     /gene="rsmI"
                     /locus_tag="VF309_06770"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7466324.1"
                     /translation="MKRDIGKAATDSGTPRAGDPRDAALEPGLYVVATPIGNLGDITL
                     RAIDVLKRVDRIAAEDTRNTRGLLNHLGISAKLVALHAHNEHRAAEALAHAIANGEAV
                     ALVSDAGTPAVSDPGAELVEAVRRAGAKVIPVPGASALTAALSASGLMFEGAVFAGFL
                     PVKGSARREKLAALAAGPWAIVLFEAPHRVAATLADLHEALGERDVVIARELTKRFET
                     ITRMPLSQAHAFVLADDDRRRGEFVLVIEGRAVEAAAGLDPRALLEALLAELPLKQAV
                     ALATRITGGKRNALYALALELKK"
     gene            2889..4073
                     /locus_tag="VF309_06775"
     CDS             2889..4073
                     /locus_tag="VF309_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX7466325.1"
                     /translation="MSRFNALLLLFVTSVVSPAFSADTKPGTTTAPQVAPRDDDIDPA
                     TGLARSSVVRPLLGAPPLPEPRRSPGPARPHIALILPTTSPALGRLADAVRQGFTAAA
                     EAGGKDATPVDVTGIENEGAALLDACRASQAAGALLVVAAMTRDAASLLVKSDCPRQP
                     VLALNDPQADANDIPANLYSVSLSLDNEAKQVALLAVAEGWRSAIVITSGTPLAKRVQ
                     EAFEREWSRAAGQVAGRLTFAGTAEDAPLVRERMANVRADMVFMALDPPEARNVRPYI
                     SGMLPLYGTSMSINPRAEAIVNLDLQGMRYVEMPWFVQPDHPAVMVYPVPRGMSIEEE
                     RLYAFGIDAYRIAQQLLHGDKRAALDGVTGRLTLEPPTRFARSLTPAEVDGGRVIPLR
                     AP"
     gene            4070..>4389
                     /locus_tag="VF309_06780"
     CDS             4070..>4389
                     /locus_tag="VF309_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX7466326.1"
                     /translation="MSSRVATGRVTAAQATGGAAEEAAARYLEERGLEIVARNYRTRL
                     GEIDLVARDGATLVFVEVRMRSSERFGGAAESIGWRKRSRLEAAARQYLARLRREPAC
                     RFDVV"
BASE COUNT          640 a         1570 c         1519 g          660 t
ORIGIN      
        1 tcttacctac tcgggcgcac gcccttcgaa cggaagtgct ccgcgtcgtc gtagaaggcc
       61 tcgatcgcgg tgtccggatg ccagcccgga atgccgagca ccggcatcgg cgccatcgac
      121 cgcggcgagg gaaagcgcgc gcggtcggag aagtgcgcgg cgacgagctc atcggcgcgc
      181 gccagccgcg cgtcccgcgg cagggagaag aacgatggcc gcgcatcgat gaacaccgtc
      241 ttcgcgacca tgccgaggta gggctcgagc gcctgctcgt agagcgcgtg gccgaacgcg
      301 aagaaccgca tgctcgcctc gacttcggcg cgccgatgcc agaagaggtc tttccactcg
      361 aacgcgggaa tcaatcgcga cagcgcgtcc gagctcgacg ccacgatcac gccgccctcg
      421 tcgaagagcg tgagcgcgtc gcgctccggg ctgcgccgcc gcgcctcgtc ctcgccgcgc
      481 tcctcgagga tcgccgcgtg ctgcgcattg atcgccgcct tcgccttcgg gaacgtgatc
      541 cacacgaggg cgttgaagag gtcgtgccag ttttccagcc gcgtctcgac ctcgcccgat
      601 tcggcgatgc gcagctcgta gtagcgccgt tcgcggtcgg tgtgcccgcg cggattcacg
      661 aagcgaagcg gcacgccgcg cgaggtgacg atcccgccgg cggcttgcgt gagctcgtcg
      721 tgcgtgggcc agcgctccgc gggaaggcgc tcgcgcgcgc cttcgagcgg cgcgtagacg
      781 ggcgagcgca ttcgctcccg ggcgcgatcc caatccacgg ggtcctagcc ggcgatgcgc
      841 caggcgatcg cctgtcccgc gcgcagcgga acgacggtct ccgcgccgaa cggatactcg
      901 tccggcacct gccactccac gcgttcgagg gtgaccttgc ccttgttgcg cggcagcccg
      961 tagaagtcgg cgccgaaatg gctcgcgaac gcctcgagct tgtcgagcgc gccggccgcg
     1021 tggaaggcct ccgcgtagag ctcgagcgcg gcgtgggcgg tgtagaggcc cgcgtgcccg
     1081 caatccgtct ccttgctgcg gcgcgtgtgc ggcgcggagt cggagccgag gaagaaccgg
     1141 ggattgcccg acatcgcagc cttcaccaac gcgcggcgat ggggctcgcg cttgagcacc
     1201 ggcaggcaat agtgatgcgg ccgcagcccg ccggagaaca tcgcgttgcg gttcaggagc
     1261 aggtgatgca cggtgacggt cgcggcgacg gtctcgggcg cgcgctcgac gaacgccgcc
     1321 gcctcgtggg tcgtgatgtg ctcgagcacg atcttcagct tcgggaagcg cttcgcgagc
     1381 ggcgcgagcg tgcgctcgag gaacacgacc tcgcgatcga agacgtccac ttcaggatcg
     1441 gtgacctcgc cgtgcatgag cagcggcatt cccgcggcct gcatcgcctc cagcgccgcg
     1501 tcgcacttgc gcaggtcggt cacgcccgag gcggagttgg tggtcgcgcc ggccgggtag
     1561 tacttcacgg ccttgatgaa tcccgcggcg cgcgccttct cgatctccga ggccggcgtc
     1621 tcgtccgtga gatagagcgt catcaggggc tcgaagcccg cgcccttcgg caacgcagcg
     1681 aggatgcgct cgcggtatgc cttcgcgtcc ttcacgctca cgaccggagg cttcaggttc
     1741 ggcatcacga tcgcgcgcgc gaattgccgc gccgtcgcac ccaccaccga ctccatgagg
     1801 tcgccgtcgc ggaaatgcgc gtgccagtcg tcggggcggg tgatcgtgat cgattcggcc
     1861 atctctggag attatagaga tgaaaactgg tgtcagagac caatttccga tacgtcagaa
     1921 agtgaaaatt ggtgtcaggt acgattttcg gatgcgtcgg catgccgccg cgcgccgccg
     1981 aaattcgtac ctgacaccaa tttcatttct tcagctcgag ggcgagcgcg tagagggcgt
     2041 tgcgcttgcc gccggtgatc ctggtggcga gcgccacggc ctgcttgagc ggcagctccg
     2101 cgagcagcgc ctcgaggagc gcgcggggat cgaggcccgc ggcggcttcg acggcgcggc
     2161 cttcgatcac gagcacgaac tcgccgcgtc ggcggtcgtc gtcggccagg acgaaagcgt
     2221 gcgcctgcga aaggggcatt cgcgtgatcg tctcgaagcg cttggtgagc tctcgcgcga
     2281 tcacgacgtc gcgctcgccg agcgcctcat gcagatcggc aagcgttgcg gccactcgat
     2341 ggggcgcctc gaacagcacg atggcccagg gccccgcggc cagcgcggcg agcttctcgc
     2401 gccgcgcgct tcccttcacg gggagaaagc ccgcgaacac cgcgccctcg aacatcaggc
     2461 cgctcgcgga aagcgccgcc gtgagggcgc tcgcccccgg caccgggatc accttcgctc
     2521 cggcgcggcg caccgcctcg accagctcgg cgcccggatc gctcacggcc ggggtgcccg
     2581 cgtcgctcac gagcgccacc gcctcgccat tcgcgatggc gtgggccagc gcctcggccg
     2641 cgcgatgctc gttgtgggca tgcagcgcga cgagcttcgc ggagattccg agatggttga
     2701 ggaggccgcg cgtgttgcgc gtgtcctcgg ccgcgatccg gtccacgcgc ttcagcacgt
     2761 cgatcgcgcg cagcgtgata tcgcccagat ttcctatggg cgtcgccacc acatataatc
     2821 ccggttccaa tgccgcgtct cgcggatctc ccgcgcgcgg cgtcccggag tccgtggccg
     2881 ccttcccgat gtcgcgtttc aacgcgctgc tccttctctt cgtcacctcg gtggtgtcgc
     2941 cggcattctc ggccgacacg aagccgggca caaccacggc cccgcaggtg gcgccgcgcg
     3001 acgacgacat cgatcccgcc acgggcctcg cgcgctcgag cgtcgtaagg ccgctcctcg
     3061 gcgcgccgcc gctgcccgaa ccgcgccgca gcccgggccc ggcgcgcccg cacatcgcgc
     3121 tcatcctgcc cacgacttcg cccgcgctcg gccggctcgc cgacgcggtg cgccagggat
     3181 tcacggcggc ggccgaggcc ggcggcaagg acgcgacgcc cgtcgacgtg accggcatcg
     3241 agaacgaggg cgccgccctt ctcgatgcat gccgcgcctc gcaggccgcg ggcgccctcc
     3301 tcgtggtcgc ggcgatgacc cgcgacgcgg cgagcctcct ggtgaaaagc gactgcccgc
     3361 gccagccggt gcttgcgttg aacgatcccc aggccgatgc gaacgatatc ccggcgaacc
     3421 tgtacagcgt ctccctctcc ctcgacaacg aggcgaagca ggtggccctc ctcgcggtcg
     3481 ccgagggctg gcgctccgcg atcgtgatca cctcgggcac gcccctcgcc aagcgcgtgc
     3541 aggaggcatt cgaacgcgag tggtcgcgcg ccgcgggaca ggtcgcgggc cgcctcacct
     3601 tcgccggcac cgccgaagac gcgccgctgg tgcgcgagcg catggcgaac gtgcgcgcgg
     3661 acatggtctt catggcgctc gacccccccg aggcgcgcaa cgtgcgaccc tacatctcgg
     3721 ggatgctgcc cctctacggg acctcgatga gcatcaaccc gcgcgcggag gcgatcgtca
     3781 acctggacct gcagggcatg cgttacgtgg agatgccctg gttcgtccag cccgatcatc
     3841 cggccgtcat ggtctacccg gttccccgcg gcatgtccat cgaggaggag cgcctctacg
     3901 ccttcggcat cgacgcctac cggatcgcgc agcagttgct gcatggagac aagcgcgcgg
     3961 cgctggatgg cgtcaccggc aggctcacgc tcgagccgcc gacgcgcttc gcgcgctccc
     4021 tcacgcccgc ggaggtcgat gggggacgcg tcatcccgct gcgagcccca tgagctcgcg
     4081 cgtggcgacg gggcgcgtca cggcggcgca ggcgacgggc ggcgccgcgg aggaggcggc
     4141 cgcgcgctat ctcgaggaac gcggcctgga gatcgtcgcg cgcaactacc gcacgcggct
     4201 gggggaaatc gacctcgtcg cccgcgacgg cgcgacactc gttttcgtcg aggtgcgcat
     4261 gcgctcgagc gagcgcttcg gcggcgcggc cgagagcatc ggctggcgca agcgctcgcg
     4321 cctcgaggcc gcggcgcggc aatacctcgc gcgccttcgc cgcgagcccg cctgccgctt
     4381 cgacgtcgt
//
LOCUS       DASVII010000520         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_491289,
            whole genome shotgun sequence.
ACCESSION   DASVII010000520 DASVII010000000
VERSION     DASVII010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491289"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..665)
                     /locus_tag="VF309_06785"
     CDS             complement(132..665)
                     /locus_tag="VF309_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7466327.1"
                     /translation="MSAAQQKAAAELIAGPRKGVVGPFIALMRSPALMDRLGRVGEYL
                     RFENALPQRLVEFAILLTARHVTNQFEWILHYPLAIKAGVSRQTLDAVSAGNRPADMA
                     KDEEVVHDFVTELLRESFVSDANYAAALGRFGEKGVIDLTATVGYFVAVCLVMNVAGT
                     PPPPSEVPPLERPATRP"
     gene            complement(698..1798)
                     /locus_tag="VF309_06790"
                     /pseudo
     CDS             complement(698..1798)
                     /locus_tag="VF309_06790"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
BASE COUNT          325 a          669 c          623 g          221 t
ORIGIN      
        1 gattcgcacg ccgcgccact cggggctgct gtcaccgatc gtgcacgcgg tgccggtcca
       61 gctcctcgcc tatcacacgg cgctgaagcg cggcaccgac gtcgacaagc cgcgcaacct
      121 cgcgaaggcc gttacggtcg agtagcggga cgctccagcg gggggacctc gctcgggggt
      181 ggcggcgtgc cggcgacgtt catcaccagg cacaccgcga cgaagtagcc cacggtcgcg
      241 gtgaggtcga tcacgccctt ctcgccgaac cgcccgagcg ccgcggcgta gttcgcgtcg
      301 gagacgaagc tctcgcgcag cagctcggtg acgaaatcgt gcaccacctc ttcgtccttc
      361 gccatgtccg cgggccgatt tcctgcgctc accgcatcaa gcgtctggcg cgagacgccc
      421 gccttgatcg caagggggta atgcaggatc cattcgaact gattggtcac gtgccgtgcc
      481 gtgagaagga tcgcgaattc gacgagccgc tgcggcagcg cgttctcgaa gcgcaggtat
      541 tcgcccacgc gccccaaccg atccatgagc gccggactgc gcatgagggc gatgaacggg
      601 cccacgacgc ccttgcgggg accggcgatc agctccgcgg ccgccttctg ttgtgctgcg
      661 ctcatgcggg aggtagccag gggcggcatg cgttctgtca tggagtcctc gcgtcggaaa
      721 caaacaacag ggcgatggcg ctcgcgatga gcgccgcgcc cgcggcgacg agcgcgacgg
      781 aatatccctc gccccgcgcg gccaggaagc tcaccaggag cgggccggcg atctggcccg
      841 cggcgaagga cgacgtcatc gcggcgatga gctcccgggc gcgaggcccc gcaacgcggc
      901 gcgcgtcctg cattcccgcc atcgtcacca ccatgaaggt tccacccacc aggagcgccg
      961 aggcgacgat gccgccgagg cccttcgccg cgagcggcac catcacgccc gccgccatcg
     1021 tgaaatgccc cgcgatccac aacgcgcgat cggacagctt gcgccgcaac gccgcggcga
     1081 tgaatgtact gagggccgcc gccgcgccga acacgggcca cgacaagccg tacaacgccg
     1141 gatcggcgag cgcctcgcgc gccatcgccg gcaggaaggt cgcgggaacg atgtagccga
     1201 agccgaatgc ggcgtagcac gcgatcatgg gaagccaggg gccatcgcga tgcgccgcgg
     1261 cctgcgttgg ggtcgacgcg gccgtgggag cgtcgtcgaa gcgattccac aacgcagcgg
     1321 cgcccccgag cgacacgatg cccaacgcga tccacgccgc acgcgacgaa gcgtgcgcga
     1381 gaagcagcgc caggcaagcc gcgcccgtga acgtgattcc gacgccgacc cccgcgaaca
     1441 atgccgcgcc cagcacggcg cgctgcggct cgcgcgcgtg cgtcgcgaac tgctcgagac
     1501 atcaggacga cgcgaacacc agcacccacg cgctcgcgac tcccgcggcg gcgcgccacg
     1561 cgagccacgg caacaaccct tccgagagtc ccatcgcgag cgtcgagatg cccacggcgg
     1621 cgaggcccat gcggatcacg cgcgccgggc ccatgggaag cgccaccgcc gagagcccgc
     1681 ccacgagata gcccacgtag ttcgccgatg cgagccagcc tccctgcacc acggtgaggc
     1741 ccgcgtcgcc gagcatcatc ggcaggatcg gcgtgaaggc gaacctgccg attcccatgg
     1801 cgatggccag cgcggcgagg ccggcgagcg cggtagtg
//
LOCUS       DASVII010000521         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_311630,
            whole genome shotgun sequence.
ACCESSION   DASVII010000521 DASVII010000000
VERSION     DASVII010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311630"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1516
                     /locus_tag="VF309_06795"
     CDS             <1..>1516
                     /locus_tag="VF309_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7466328.1"
                     /translation="DLNKNGKLDPYEDRRQPTAVRVENLLGQMTLDEKAGTMFHTFVW
                     PGKNGEIAGPLQVLNLLPVEVALFEKKITFFNLFGLGTAAETAHWLNAVQRLAERTRL
                     GIPVTFSSDPRHSAALQKSVAAFAMPEFSQWPEPIGFAAIGDSALVVEFGRFAAQDYR
                     AIGIQVALHPMADVATEPRWGRISGTFGEDANLAAKLTAAYVRGMQGDSLSATSVATV
                     TKHFAGGGPQEDGWDPHFRYGKNQVYPGHNLAYHLIPFRAAIAAGTALIMPYYGVPVG
                     LTKEDVGFSFSREMISGLLRDSLGFKGIVLTDWGLLTPTKILGIPLERFYPIAAVKDY
                     GVENLTPPYRAKKALDAGVDQFGGEMHPEYLLQLVREKAIPESRLDESVRRLLTLKFE
                     LGQFDNPYVDVGAVASRVGTPEARRLGNETQRRAQVLLTNKETNGKGILPLKPGTRIY
                     IQNLDAAVAAKYGTVVKTPEEADVAILNLDPPFDHDRGSLMHQGRLYYTADELKP"
BASE COUNT          276 a          481 c          500 g          259 t
ORIGIN      
        1 cgacctgaac aagaacggaa agctggatcc ttatgaggat cgccggcagc cgacagccgt
       61 ccgcgtggag aacctgctgg gtcagatgac cctcgacgag aaggccggca cgatgtttca
      121 caccttcgtg tggccgggaa agaacggtga gatcgcgggc ccgctccagg tgttgaacct
      181 gctgccggtc gaggtcgcgc tcttcgagaa gaagatcacc ttcttcaacc tgttcgggct
      241 cggcaccgcc gcggagaccg cgcactggct caacgcggtg cagcgcctgg cggagcggac
      301 gcgtttgggc atccccgtca ctttcagctc cgaccccagg cactccgccg cgcttcagaa
      361 gtcggttgcc gcgttcgcga tgcccgagtt ctcacagtgg ccggagccga tcggattcgc
      421 ggcgatcggc gacagcgcgc tggtcgtgga gttcggtcgc tttgcggcgc aggactatcg
      481 cgcgatcggc attcaggtcg cgctgcaccc catggcggat gtcgcgaccg agccgcgctg
      541 ggggcgcatc agcgggacgt tcggtgagga cgccaacctc gccgcgaaat tgacggcggc
      601 gtacgtccgc ggcatgcagg gcgactcgct ctcggcgacc agcgtcgcca ccgtcaccaa
      661 gcacttcgcg ggcggcggtc cccaggagga cggttgggat ccgcatttcc gatatggcaa
      721 gaaccaggtt tatcccgggc acaacctcgc gtaccacctg atcccctttc gcgcggcgat
      781 cgcggccggc accgcgctga tcatgccgta ctacggcgtg cccgtcggtc tcaccaaaga
      841 ggacgtcggc ttcagcttca gccgcgagat gatttccggc ctgctgcgcg actcgctcgg
      901 tttcaagggg atcgtcctca cggactgggg tcttctcacg ccaacgaaga tcctgggcat
      961 ccctctcgag cggttctatc cgatcgccgc cgtgaaggat tacggcgtgg agaatctgac
     1021 tccgccgtac cgggccaaga aggcgctcga tgcaggcgtg gaccagttcg gcggcgagat
     1081 gcatcccgag tacttgctgc agctcgttcg cgagaaggcg atcccggagt cgcggctcga
     1141 cgaatcggtg cgcaggctgc tcacgctcaa attcgagctg gggcaattcg acaacccgta
     1201 cgtggatgtc ggtgccgttg catcgcgcgt cggaactcca gaggcaaggc ggctgggtaa
     1261 cgaaacacag cggcgcgctc aggtgctgct gacgaacaag gagacgaacg ggaaggggat
     1321 cctgccgctc aagccgggca cgcgcatcta catccagaat ctcgatgcgg cggtcgccgc
     1381 gaaatacggg accgttgtga agacgcccga ggaagcggac gttgccatcc tgaacctgga
     1441 tccgccgttc gaccacgatc gcggatcgct gatgcatcag ggccggctgt actacaccgc
     1501 ggatgagttg aagccg
//
LOCUS       DASVII010000522         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_707402,
            whole genome shotgun sequence.
ACCESSION   DASVII010000522 DASVII010000000
VERSION     DASVII010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707402"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF309_06800"
     CDS             complement(<1..207)
                     /locus_tag="VF309_06800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7466329.1"
                     /translation="MPAPQVSILIPAYNPRFFAEAFASARAQKHPSFEIVVRDDSPGT
                     LIEECVLAANDPRVRYLRNPARLGF"
     gene            complement(248..619)
                     /locus_tag="VF309_06805"
     CDS             complement(248..619)
                     /locus_tag="VF309_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466330.1"
                     /translation="MAYSIRVDAERRVVLVVVRDPCDASGAGALITSARQAAAESGFG
                     VLYDIRAARQGDVQSSDVFWWPRSIPVLQDPAARRVRAAVLHGPQHRALVQFWETAFR
                     NLGFPASAFEDEELALQWLSG"
     gene            complement(627..935)
                     /gene="ftsB"
                     /locus_tag="VF309_06810"
     CDS             complement(627..935)
                     /gene="ftsB"
                     /locus_tag="VF309_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEX7466331.1"
                     /translation="MKSLAFILTGLLLALQYPLWLGKGSWSRVWELDRGLAQQRDTNM
                     KLKARNDALDAEVRDLKQGLEALEERARLELGMIRKDEVFYQVVPAAEAGAASRPPAR
                     "
     gene            complement(932..>1853)
                     /gene="eno"
                     /locus_tag="VF309_06815"
     CDS             complement(932..>1853)
                     /gene="eno"
                     /locus_tag="VF309_06815"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7466332.1"
                     /translation="AAEESGLSLYRYFGGASPKRMPVPQMNVVNGGAHANNSLDIQEF
                     MIIPSGMATFRDALRCGAEIFQTLKKMIDKAGMPTTVGDEGGFAPSLPNNEAAIQWIL
                     RAIEEAGYLAGENVMIGLDCAASEFFKDGKYRFESEKLAYSPAQMVDLLAAWCDKYPI
                     ISIEDGMAEQDWDGWELLTRRLGKDVQIVGDDIFVTNTRILAEGIQRGVANSILIKVN
                     QIGTLSETFEAIAMAKRAGYTSVISHRSGETEDSTIADIAVGTNAMQIKTGSLSRSDR
                     TAKYNQLLRIEEELGEGATYAGRDAFHQLK"
BASE COUNT          290 a          615 c          628 g          320 t
ORIGIN      
        1 gaacccgagc cgcgcggggt tgcgcaggta gcgcactcgc ggatcgttcg ccgcgagtac
       61 gcactcctcg atgagggtgc cgggcgaatc gtcgcgcacc acgatctcga acgagggatg
      121 cttctgcgcc cgcgcgctcg cgaaggcctc ggcgaagaag cgcggattgt aggcgggaat
      181 caggatcgag acctgcgggg cgggcatggc gctcggccac ctcctcggcg cgtagtgtgg
      241 ggtcgattca gccggaaagc cactgcaacg cgagctcctc gtcctcgaag gcgctcgccg
      301 ggaaccccag gttgcgaaaa gcggtttccc agaattgcac gagggcgcga tgctggggac
      361 cgtggagcac ggcggcgcgc acgcgccgcg ccgcgggatc ctgcagcacc ggaatgctgc
      421 gcggccacca gaagacgtcg ctcgactgca cgtcgccctg ccgtgcggcg cgaatgtcgt
      481 agagcacgcc gaaaccgctt tcggcggcag cctggcgggc cgaggtgatc agggccccgg
      541 cgcccgacgc gtcgcatgga tcccgcacca caacgagcac gacgcggcgc tccgcgtcga
      601 cgcggatcga ataggccatg cgggggttac cgcgccgggg gccggcttgc ggcgccggcc
      661 tcggcggccg gaacgacctg gtagaagacc tcgtccttgc ggatcatgcc cagctcgagt
      721 cgcgcgcgct cctcgagcgc ttccaatccc tgtttcaaat ccctcacctc tgcgtcgagc
      781 gcgtcgttgc gcgccttgag tttcatgttg gtgtcgcgct gctgcgcgag accccggtcg
      841 agctcccaca ccctcgacca gcttcccttg cccagccaca gcggatactg cagcgcgaga
      901 agaagtccgg tgagaatgaa ggcgagcgat ttcacttcag ttgatggaag gcgtcgcggc
      961 ccgcgtaggt cgcgccctcg cccagctcct cctcgatgcg cagcagctga ttgtacttcg
     1021 cggtgcggtc ggagcgcgag agcgatcccg tcttgatctg catcgcgttg gtgccgaccg
     1081 cgatgtcggc gatggtcgag tcctcggtct cgcccgagcg gtgcgagatg accgaggtgt
     1141 agcccgcgcg cttcgccatc gcgatcgcct cgaaagtctc cgagagcgtg ccgatctggt
     1201 tcaccttgat caggatcgag ttggcgacgc cgcgctggat gccctcggcg aggatgcgcg
     1261 tgttggtgac gaagatgtcg tcgcccacga tctgcacgtc cttgccgagg cggcgcgtga
     1321 ggagctccca gccgtcccag tcctgctcgg ccatgccgtc ctcgatcgag atgatcgggt
     1381 acttgtcgca ccaggcggcg aggaggtcga ccatctgcgc cggcgaatac gcgagcttct
     1441 cggactcgaa gcgatacttg ccgtccttga agaactcgct cgcggcgcag tcgaggccga
     1501 tcatcacgtt ctcgcccgcg aggtagcccg cttcttcgat ggcgcgcagg atccattgga
     1561 tcgccgcttc gttgttgggc aggctcgggg cgaaaccgcc ctcgtcgccg acggtggtgg
     1621 gcatgcccgc cttgtcgatc atcttcttca gcgtctggaa gatctccgcg ccgcaacgca
     1681 gcgcgtcgcg aaaggtcgcc attcccgacg ggatgatcat gaactcctgg atgtcgagag
     1741 agttgttcgc gtgcgctccg ccgttcacca cgttcatctg cggcacgggc atgcgcttgg
     1801 gggaggcgcc gccgaagtag cggtagagcg acagccccga ctcctcggcc gcc
//
LOCUS       DASVII010000523         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_707458,
            whole genome shotgun sequence.
ACCESSION   DASVII010000523 DASVII010000000
VERSION     DASVII010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707458"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1356
                     /locus_tag="VF309_06820"
     CDS             370..1356
                     /locus_tag="VF309_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7466333.1"
                     /translation="MIAFVLRRLLQGVIVMLVVGLIAFSLFNFVGDPVSLMLPPEATQ
                     ADRDAMRQALGLDKPFYVQFVTFIGNAVQGNFGISLRLGRPVSTLLVERLPATLELAF
                     TAAMIGLLVGIPVGVYTALKRDSWVSRALLTTSLVGVSLPNFLIGIFLILIFAVWLGW
                     LPSFGRGDTVKIGLWSTGFLTKSGLKSLILPAITLGFFPVTLIMRLVRAEMLEVLRTD
                     YIKFARARGLSDRAVNFGHALKNTLVPVITITGLQLGGIIAFAIITETVFQWPGMGLL
                     FVQAVQFADIPVMAAYLCLIGLIFVVINLVVDMLYYLVDPRLRAERGTSGGH"
     gene            1356..>1828
                     /locus_tag="VF309_06825"
     CDS             1356..>1828
                     /locus_tag="VF309_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466334.1"
                     /translation="MDAVRRFFDGDVFYSFKHSPLTITAAAIVVACIFGATFAPFVAP
                     HNPFDLKTLNLLDAFTPPSFTGHGNPSYLLGTDDQGRDVLSTIIFGSRMSLVIGLGAT
                     FLAMVFGVSMGLMSGYIGGKFDAFIMRVADIQLSFPAILIALLIDGVARAVLPKGS"
BASE COUNT          249 a          623 c          573 g          383 t
ORIGIN      
        1 cgatccagtg agttttgctt cgttcccgcg cgggcgggaa tccacgcgac aacccggtaa
       61 tctgccaggc gtggaagggg cggttcttct cgaaccggca tcgactcaat gagacctctc
      121 cgacttgaag cgacgagtag ggcttgggga aatgttcgag agggcgccca ggtggttgtg
      181 aaggggttcg ccgccgccac ttgtccacgg gtatcgcatc catccgagtc actgccgccc
      241 gggtcgaaca tttccccgcc ctcactcgtc gctgatcgcg ccctcgggtg cgcgcggtga
      301 atgccccttg aatcccacgc gtgagcttgc ggatgtgacg ctgagctccc gccgccgcgg
      361 gaacgacgcg tgatcgcttt cgtcctgcgc cggctcctgc agggcgtcat cgtgatgctg
      421 gtcgtggggc tcatcgcctt ctcgctcttc aacttcgtgg gcgatccggt gtcgctcatg
      481 ctgccgcccg aagcgaccca ggccgatcgc gacgcaatgc gccaggcgct cggcctcgac
      541 aagcccttct acgtccagtt cgtgacgttc atcggcaatg cggtgcaggg caacttcggc
      601 atttcgcttc gcctcgggcg gccggtttcc acgctgctgg tggaacgcct tccggcgacg
      661 ctcgagctcg cgttcaccgc ggcgatgatc gggctcctcg tcggcattcc cgtgggcgtg
      721 tacacggcgc tgaagcgcga ctcgtgggtt tcgcgcgcgc tcctcacgac ctccctcgtc
      781 ggcgtgtcgc tgccgaattt cctcatcggc atcttcctca tcctcatctt cgcggtgtgg
      841 ctcggctggc ttccctcctt cgggcgcggc gacacggtga agatcggcct gtggtccacg
      901 ggcttcctca cgaagtcggg gctgaagtcg ctgatccttc ccgcgatcac cctgggcttc
      961 ttcccggtga cgctcatcat gcggctggtg cgcgcggaga tgctcgaggt gctgcgcacc
     1021 gactacatca agttcgcgcg cgctcggggg ctctcggacc gcgcggtgaa cttcggccac
     1081 gcgctgaaga acacgctcgt cccggtgatc accatcacgg ggctgcagct gggcggcatc
     1141 atcgccttcg cgatcatcac cgaaaccgtg ttccaatggc ccggcatggg gctgctcttc
     1201 gtgcaggccg tgcagttcgc cgacattccg gtgatggccg cgtacctttg cctgatcggg
     1261 ctcatcttcg tggtgatcaa cctcgtcgtg gacatgctgt actacctcgt cgatccgcgg
     1321 ctgcgcgccg agcgcggcac gagtgggggg cactgatgga cgctgtgcgg cgcttcttcg
     1381 acggcgacgt cttctacagc ttcaagcact cgcccctgac gatcacggcg gcggcgatcg
     1441 tggtcgcgtg cattttcggc gcgaccttcg cgcccttcgt cgccccgcac aatccgttcg
     1501 acctgaagac gctgaacctc ctcgacgcct tcacgccgcc ctccttcacc gggcacggca
     1561 atccgagcta cctcctcggg accgacgacc aggggcgcga cgtgctctcg acgatcatct
     1621 tcggctcgcg catgtcgctc gtgatcgggc tgggggcgac gttcctcgcg atggtgttcg
     1681 gcgtgagcat gggcctcatg agcggctata tcggcggcaa gttcgacgca ttcatcatgc
     1741 gcgtggccga catccagctt tcgttcccgg cgatcctcat cgcgctcctc atcgacggtg
     1801 tcgcgcgcgc ggtgcttccg aagggctc
//
LOCUS       DASVII010000524         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_635280,
            whole genome shotgun sequence.
ACCESSION   DASVII010000524 DASVII010000000
VERSION     DASVII010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635280"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VF309_06830"
     CDS             <1..1044
                     /locus_tag="VF309_06830"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX7466335.1"
                     /translation="MSIKDLRERLGKIVCGYTRDQKPILAKDLKTHGAMAALLKDALA
                     PNLVQTLEHSPAFIHGGPFANIAHGCNSVLATKTALKLGDYVVTEAGFGADLGAEKFV
                     DIKCRKAGLKPAAVVLVATVRALKYHGGVEVKELAKENLEALAKGITNIERHVNNIRN
                     NYGLPCVVSINHRTEDTDAEIKMLQEKMAHHGAKVIVARHWAEGGKGAVEVAKEVVRL
                     CDEPNNFKFVYEEQATLWDKMKAIATKIYGASDITADSKVRAQIKKLQESGYGHYPIC
                     VAKTQYSFSTDPQLRGAPSGHVVNVREVRLAAGAEFVVMICGDIMTMPGLPKVPSAVS
                     IDVDDDGRITGLF"
     gene            1112..1870
                     /locus_tag="VF309_06835"
     CDS             1112..1870
                     /locus_tag="VF309_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7466336.1"
                     /translation="MRLKNRVAIVTGAGSGFGEAIARRYADEGAQVVVNDIAGDAAER
                     VAKEISAQGGSAAAHVGDVSRDAQVAGLVKSALDRFGRLDIIVNNAGTTHRNRPMLEV
                     GEEEFDRIYAVNVKPLFLTAKHAVPHFRKQGGGCFVTIASTAGVRPRPGLTWYNGSKG
                     AAIVTSRSMAAELGPDKIRVNVINPVIGETGLLEMFMGMPDTPENRKKFLAGIPMGRF
                     SKPLDVANAALFLASDEAAFITGACVEVDGGRCV"
     gene            1867..>2418
                     /locus_tag="VF309_06840"
     CDS             1867..>2418
                     /locus_tag="VF309_06840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7466337.1"
                     /translation="MSAMQSVGKYQLLRELGKGATGKVYLAEDPFNKRKVAIKVAFPE
                     ALKHSEDGAFYKSMFLNEAALAGKLNHPHITQIYDAVVEDKYSYIVMEYVEGGTLEKF
                     CKPEALLETRDVAEIAFKCVRALAFAQTLGLTHRDIKPGNILHSDGTEIKIADFGASI
                     NKVSDRTVITNVGSPAYMAPELVT"
BASE COUNT          468 a          776 c          808 g          366 t
ORIGIN      
        1 atgagcatca aggatctccg ggagcgcctc ggcaagatcg tgtgcggcta cacgcgcgac
       61 cagaagccga tcctcgcgaa ggatctgaag acccacgggg cgatggcggc gctgctcaag
      121 gatgcgctcg cgcccaacct cgtgcagacg ctcgagcact cgcccgcgtt catccatggc
      181 ggtcccttcg cgaacatcgc gcacggctgc aactcggtgc tcgcgacgaa gacggcgctg
      241 aagctcggcg actacgtcgt gaccgaggcg ggattcggcg cggacctcgg cgccgagaag
      301 ttcgtcgaca tcaagtgccg caaggcggga ttgaagccgg ccgcggtggt gctggtggcc
      361 acggtgcgcg cgttgaagta ccacggcggc gtggaagtga aggagctcgc caaggaaaat
      421 ctcgaggcgc tcgccaaggg cattaccaac atcgagcgcc acgtgaacaa catccgcaac
      481 aactatggcc tgccgtgcgt cgtgtcgatc aaccaccgca ccgaggatac ggacgccgag
      541 atcaagatgc tgcaggagaa gatggcgcac cacggcgcca aggtgatcgt cgcgcgccac
      601 tgggccgagg gcggcaaggg cgcggtcgag gtcgcgaagg aagtggtgcg cctgtgcgac
      661 gagccgaaca acttcaagtt cgtctacgag gagcaggcga ccctctggga caagatgaag
      721 gcgatcgcga ccaagatcta cggcgcatcc gacatcaccg ccgattcgaa ggtgcgcgcg
      781 cagatcaaga agctgcagga gtcgggctac gggcactatc cgatctgcgt ggcgaagacg
      841 cagtattcgt tctcgacgga tccgcagttg cgcggcgcgc cctcggggca cgtggtgaac
      901 gtccgcgagg tgcgcctggc ggcgggcgct gaattcgtcg tgatgatctg cggcgacatc
      961 atgacaatgc ccgggttgcc caaggtgccg tcggcggtga gcatcgacgt ggacgacgac
     1021 gggcgcatca ccggcctgtt ctgaatccag cgaaagagcg actcccgggc tccggatttg
     1081 cttccggggc cgggccgtat aatcccgttc catgagattg aagaacaggg tggcgatcgt
     1141 caccggggcg ggttcgggct tcggcgaggc catcgcgcgc cgctatgcgg atgagggcgc
     1201 gcaagtcgtc gtgaacgata tcgccggcga tgccgccgag cgtgtcgcga aggaaatcag
     1261 cgcgcagggc ggcagcgcgg ccgcccatgt gggcgacgtt tcgcgcgacg cccaggtcgc
     1321 cggcctcgtg aaatccgccc tcgatcgctt cggccgcctc gacatcatcg tcaacaatgc
     1381 gggcaccacc catcgcaacc gcccgatgct cgaggtcggc gaggaagagt tcgaccgcat
     1441 ctacgcggtg aacgtgaagc cgctcttcct caccgcgaag cacgcggtgc cgcatttcag
     1501 gaagcagggc ggcggctgct tcgtgaccat cgcctccacc gcgggcgtgc gcccccgtcc
     1561 ggggctgacc tggtacaacg gctcgaaggg cgccgcgatc gtgacctcgc gctccatggc
     1621 cgcggagctc gggcccgaca agatccgggt gaacgtcatc aacccggtga tcggcgagac
     1681 cggcctcctc gagatgttca tgggcatgcc cgacacgccg gagaaccgca agaagttcct
     1741 cgccgggatt cccatgggcc gcttctcgaa gccgctggac gtcgccaacg cggcgctatt
     1801 cctcgcctcc gacgaggcgg ccttcatcac cggcgcttgc gtcgaagtcg acggaggccg
     1861 gtgcgtatga gcgcgatgca gtccgtcggc aagtaccagc tcctgcgcga gctcggcaag
     1921 ggcgcgaccg gcaaggtcta cctggcggaa gatcccttca acaagcgcaa ggtcgcgatc
     1981 aaggtcgcgt tccccgaggc gctgaagcat tcggaggatg gcgctttcta caagagcatg
     2041 ttcctcaacg aggcggcgct cgccgggaag ctcaaccacc cgcacatcac gcagatctac
     2101 gacgcggtgg tcgaggacaa gtacagctac atcgtgatgg agtacgtcga gggcgggacc
     2161 ctcgagaagt tctgcaagcc cgaggcgctc ctcgagacgc gtgacgtggc ggagatcgcc
     2221 ttcaaatgcg ttcgcgccct cgccttcgcg cagacgctgg gcctcaccca ccgcgacatc
     2281 aagccgggca acatcctcca tagcgacggc accgagatca agatcgccga cttcggcgcg
     2341 tcgatcaaca aggtgagcga ccgcacggtg atcaccaacg tcggctcacc cgcgtacatg
     2401 gcgccggagc tggtcacc
//
LOCUS       DASVII010000525         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_167734,
            whole genome shotgun sequence.
ACCESSION   DASVII010000525 DASVII010000000
VERSION     DASVII010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167734"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VF309_06845"
     CDS             <1..919
                     /locus_tag="VF309_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466338.1"
                     /translation="PGRFDAKVLQWPAIKAYAERLKLADGDAIARRNLGTAASIDAIF
                     KSYGAPINSDYFPIVDQKAAQTRFIQARVTAMTDLQLSTLPLLEMLDASFRPSSKRPD
                     TYPWALADAASVTAWTLRDTSLGERAPADAAEYSDPFHLAAQMVGVWGTSCPANISFD
                     DILPHLVSTAGASAQLPSGAAVAMWKRIGDSACARRVSPANRLWFDLFSAVAARDPDA
                     MSATGAKLLDALHGTRSAASEYAFLATVAGHACRGRAPEIDRLYQLAASDWVRQGQHA
                     LEIRYLYFLAHDAGARHDNGEGCVTAAKY"
     gene            complement(921..1700)
                     /gene="soxA"
                     /locus_tag="VF309_06850"
     CDS             complement(921..1700)
                     /gene="soxA"
                     /locus_tag="VF309_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX7466339.1"
                     /translation="MGIGNANATPESVKGEIAARLQHELPDAKPADYALGSAGFDAEL
                     RARLEENAAAGTDAVTAGRALWTAKFRDGRTLASCFPNGGRRIAQNYPQYDSRLKRVV
                     TLEMAVNQCLKAHGQPLYEPADPATMGLAIAYVRSLADGQKIAVRVPPAAEPKFAGGS
                     RLYFTRMGQRNFACASCHIENAGKHYGDEALSPAIGQATHWPVIRGGAPVTLHARMRE
                     CLELMGAAPFAPGSEELNHLEYFLAYLSNGLPLKANAWREK"
     gene            complement(1742..2080)
                     /gene="soxX"
                     /locus_tag="VF309_06855"
     CDS             complement(1742..2080)
                     /gene="soxX"
                     /locus_tag="VF309_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX7466340.1"
                     /translation="MRFAAAVVACASASAFAAAPRAPALDLFVRADKGNCIACHQLPD
                     GAGPATRADVGPRLEGPRMRALGKDALRRWLVDPMRANPDTVMPPFGRHRLLDDAEID
                     RLVQFLETLP"
     gene            complement(2080..>2855)
                     /locus_tag="VF309_06860"
     CDS             complement(2080..>2855)
                     /locus_tag="VF309_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466341.1"
                     /translation="TTERIADMQDRLQSVAAKMVPDSFDYRLARAKLRAMSGSPSEAV
                     TLFRTALEDQSVLRPREDVYGLAYAQRRASDLDGAWKTLESIRARPAEAKALPVFGVA
                     VPPHPAIELLAGQVKADMHKEDEAEAIYRAALRDAPHNRGLAYAYIDLLLRTDRAGVA
                     LSDLEERIRYTPEDWRMYELQSRAFEAVKRPIAQHRAQAEAYFRRGNLAAAVDQLEIA
                     VKQTRGSDFYELSIAESRLRDLRSQLEIEKAAEKALKIT"
BASE COUNT          415 a          995 c         1003 g          442 t
ORIGIN      
        1 cccgggccgg ttcgacgcga aggtgctgca atggccggcc atcaaggcat acgccgagcg
       61 cctgaagctc gccgatggcg acgccatcgc gcggcgcaac ctgggaaccg cggcatcgat
      121 cgacgcgatc ttcaagtcct acggcgcgcc catcaactcc gactactttc cgatcgtcga
      181 ccagaaggcc gcgcagacgc gcttcatcca ggcgcgggtg acggcgatga ccgacctgca
      241 gctctcgacg ctgccgctcc tcgagatgct cgacgcgtcg ttccgcccct cgtcgaagcg
      301 ccccgacacg tatccgtggg cgctcgccga tgcggcctcg gtgacggcgt ggacgctacg
      361 cgacacgtcg ctcggcgagc gcgcgcccgc ggacgcggcc gaatactccg atcccttcca
      421 cctcgccgcg cagatggtgg gcgtgtgggg aacgtcctgc cccgcgaaca tttccttcga
      481 cgacatcctt ccgcacctgg tgagcaccgc gggcgcgagc gcgcagctgc cgtcgggcgc
      541 ggcggtcgcg atgtggaagc gcatcggcga cagcgcctgc gcgcggcgcg tgtcccccgc
      601 gaaccggctg tggttcgacc tcttctcggc ggtggcggcg cgcgatcccg acgcgatgtc
      661 ggcgaccgga gcgaaactcc tcgacgccct gcatggaacg cgaagcgccg cgtcggagta
      721 tgcgttcctc gccaccgtcg ccgggcatgc gtgccgggga cgcgcgccgg agatcgacag
      781 gctctaccag ctggcggcga gcgattgggt ccgccagggg cagcatgcgc tggaaattcg
      841 ctacctgtac tttcttgcgc acgatgccgg cgcgcggcac gacaacggcg aaggctgcgt
      901 gaccgcggcg aagtactgag ctatttctcg cgccaggcgt tggccttgag gggcagcccg
      961 ttggagaggt aggcgaggaa gtactcgagg tggttcagct cctcggagcc cggggcgaac
     1021 ggggccgcgc ccatgagctc gaggcattcg cgcattcgcg catgcagcgt caccggcgcg
     1081 ccgccgcgaa tcaccggcca atgcgtggcc tgcccgatcg cgggcgacag cgcctcatcg
     1141 ccatagtgct tgcccgcgtt ctcgatgtgg cacgacgcgc aggcgaagtt gcgctggccc
     1201 atgcgcgtga aatagaggcg gcttcccccc gcgaacttcg gctccgcggc cggcggcacg
     1261 cgcaccgcga tcttctgccc gtcggcgagc gagcgcacgt acgcgatggc gaggcccatc
     1321 gtcgcgggat cggcgggctc gtagagcggc tgcccgtggg ccttgaggca ttggttgacg
     1381 gccatctcga gcgtcaccac gcgcttcagc cgcgagtcgt actgcggata gttctgcgcg
     1441 atgcgccgtc ccccgttggg gaagcacgat gcgagcgtgc gcccgtcgcg gaacttcgcg
     1501 gtccacaacg cgcggccggc cgtgaccgca tcggtgcccg ccgccgcgtt ctcctcgagg
     1561 cgcgcgcgaa gctccgcgtc gaatcccgcc gagccgagcg cgtagtcggc gggcttcgca
     1621 tccggaagct cgtgctgcag ccgcgctgcg atctcgcctt tcaccgattc cggtgtggcg
     1681 ttggcgttcc caatgcccaa ggcgaggatg cagatacaaa gccctttgcc cgccatggcg
     1741 ctcacggtag cgtctcgagg aattgaacaa gccggtcgat ctccgcgtca tcgagcagcc
     1801 gatggcgccc gaagggcggc atgacggtgt cgggattggc gcgcatcgga tcgacgagcc
     1861 accggcgcaa cgcgtccttt ccgagggcgc gcatgcgcgg tccctcgagg cgcgggccca
     1921 cgtcggcgcg cgtcgcgggc cccgcgccgt cgggcaattg gtggcacgcg atgcagttgc
     1981 ccttgtcggc gcgcacgaaa aggtcgagcg ccggggcgcg aggcgcggcg gcgaacgcgc
     2041 tcgcgctcgc acaggcgacg accgctgcgg cgaagcgcat caagtgatct tcaacgcctt
     2101 ctcggcggcc ttctcgatct cgagctgcga gcgcaggtcg cgcagccgcg actcggcgat
     2161 cgacagctcg tagaaatcgc tgccgcgcgt ctgcttcacc gcgatctcga gctggtccac
     2221 ggcggcggcg aggttgccgc gccggaaata cgcttcggcc tgggcgcgat gctgcgcgat
     2281 cggcctcttt acggcctcga acgcgcgcga ctgcagctcg tacatgcgcc agtcctcggg
     2341 ggtgtagcga atgcgctcct cgagatcgga gagcgccacg ccggcgcggt cggtgcgaag
     2401 caacaggtcg atgtaggcat aggcgaggcc gcgattgtgc ggggcgtcgc gaagcgccgc
     2461 gcgatagatc gcctccgcct cgtcttcctt gtgcatgtcg gccttcacct gcccggcaag
     2521 gagctcgatc gcgggatggg gcggcaccgc cacgccgaac acgggcaggg ccttcgcttc
     2581 ggccgggcgg gcgcgaatcg actcgagcgt cttccacgcc ccgtccaggt cgctcgcgcg
     2641 ccgctgcgcg taggcgaggc cgtagacgtc ctcgcgcggg cgcagcacgc tctggtcttc
     2701 gagcgccgtg cgaaagagcg tcacggcttc cgacggcgag ccgctcatgg cgcgcagctt
     2761 cgcgcgcgcg agccggtagt cgaacgagtc gggcaccatc ttcgcggcga cgctctgcag
     2821 ccgatcctgc atgtccgcga tgcgctcggt cgtga
//
LOCUS       DASVII010000526         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_24864, whole
            genome shotgun sequence.
ACCESSION   DASVII010000526 DASVII010000000
VERSION     DASVII010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24864"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF309_06865"
     CDS             complement(<1..387)
                     /locus_tag="VF309_06865"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466342.1"
                     /translation="MARNAHSPPYDDALATQMREAHDKFAESMGGEGPSQQVMSGFAE
                     AYRAWLEAMSAKPETMMDLQGRYMQEQMRLWMKTMEPPDGHEEEVHDKRFSGKEWSEL
                     PMFRYFRDSYLLTSKMMMQAVEEAALD"
     gene            complement(424..1215)
                     /locus_tag="VF309_06870"
     CDS             complement(424..1215)
                     /locus_tag="VF309_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7466343.1"
                     /translation="MSILAYIVASCLLGTVLSIALAALVAFRAKARWISTLVSYAVGA
                     LLGAAFLDILPHLFEGNSHPARTAAFILFGLLVFFMLEKLLLWRHHHHQSDEEEAEAE
                     AQMRESGHDHGRTGWMIIFGDAFHNFTDGVIIAGAFLADVKIGLVTSLAIIAHEIPQE
                     IGDFVVLLHSGFTKRSALFWNLVSGMSSVAGGVIAYFALSHVTDLIPDILAFAVASMI
                     YVAVADLIPGLHKTTAVKESLYQVAFIALGIASIWLIHGVLFPEV"
     gene            complement(1223..>1943)
                     /gene="pgeF"
                     /locus_tag="VF309_06875"
     CDS             complement(1223..>1943)
                     /gene="pgeF"
                     /locus_tag="VF309_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX7466344.1"
                     /translation="IAPDWPAPTRVRAFVTTRHGGVSGGEYASLNLGARGGDDPRNVA
                     SNRLVVRDHLPSMPRWMAQAHGTAVCDLDAIGDHDVATADAAVTGTPGTVGVVLTADC
                     MPLFVADRGGTRVGVAHAGWRGMAAGVIENAVSSLGVDPREVVAWMGPTIGPTAFEVG
                     PEVREAFVAADPGARAAFMPHAAGKFMADLYALARRRLARAGVREVHGGGFCTYRETQ
                     RFFSYRRVAESGRMGAFIWME"
BASE COUNT          335 a          649 c          669 g          290 t
ORIGIN      
        1 gtcgagcgcc gcctcctcga ccgcctgcat catcatcttc gaggtgagca ggtacgaatc
       61 gcggaagtag cggaacatcg gaagctcgct ccactccttg ccgctgaagc gcttgtcgtg
      121 gacctcctcc tcgtggccgt cgggaggctc catggtcttc atccagaggc gcatctgctc
      181 ctgcatgtag cgtccctgca ggtccatcat cgtctccggc ttcgccgaca tcgcctcgag
      241 ccacgcgcga taggcctcgg cgaagccgct catcacctgc tgcgagggcc cctcgccgcc
      301 catcgattcg gcgaacttgt cgtgcgcctc ccgcatctgc gtggccaatg catcgtcgta
      361 gggcggggaa tgggcgtttc gggccatggg agtgggagcg gggcaagccg cccgttgcga
      421 aacctagact tccggaaata atacgccgtg gatcagccag atgctcgcga tccccagcgc
      481 gatgaaagcc acctggtaga gcgattcctt cacggcggtc gtcttgtgca gcccggggat
      541 caggtcggcg accgcgacgt agatcatgct cgccacggcg aaggcgagta tgtccgggat
      601 caagtccgtg acgtggctca gggcgaagta ggcgatgacg cccccggcca ccgacgacat
      661 gcccgagacg agattccaga aaagggcgct gcgcttggtg aagcccgagt gcaggagcac
      721 cacgaagtcg ccgatttcct gcgggatctc gtgcgcgatg atggcgagcg aggtcaccag
      781 gccgatcttc acgtcggcga ggaacgcccc ggcgatgatg acgccatcgg tgaagttgtg
      841 gaacgcgtcg ccgaagatga tcatccagcc ggtgcggccg tggtcgtggc ccgactcgcg
      901 catctgcgcc tcggcttccg cctcctcctc gtcgctctgg tggtgatggt ggcgccagag
      961 gagcagcttc tccagcatga agaagaccag gagcccgaag aggatgaagg ccgcggtccg
     1021 cgccgggtgg ctgttgccct cgaagaggtg cggaaggatg tcgaggaagg cggcgccgag
     1081 gagcgcgccc acggcgtagc tcacgagcgt ggagatccag cgcgccttgg cgcgaaacgc
     1141 gacgagcgcg gcaagcgcga tcgaaaggac ggtgccgagg aggcacgagg cgacgatgta
     1201 ggcgaggatg ctcattgccg gattattcca tccaaatgaa agcccccatc cggccgctct
     1261 cggcaacccg gcgataggag aagaatcgct gcgtctcgcg ataggtgcag aatcccccgc
     1321 cgtgcacctc gcgcacgccg gcgcgcgcga ggcgccggcg cgcgagcgca taaagatccg
     1381 ccatgaattt tcccgcggcg tggggcatga acgcggcgcg cgcgcccggg tcggcggcca
     1441 cgaaggcctc gcgcacctcg ggaccgacct cgaacgccgt ggggccgatc gtgggcccca
     1501 tccacgcgac cacctcgcga ggatcgacgc cgagcgagga caccgcgttc tcgatcacgc
     1561 ccgccgccat cccgcgccag ccggcgtggg cgacgccgac gcgcgtgccg ccgcggtcgg
     1621 cgacgaagag cggcatgcag tcggcggtaa ggacgacgcc caccgtcccg ggtgttccgg
     1681 tcaccgcggc atcggccgtc gcgacgtcgt gatccccgat cgcgtcgaga tcgcagaccg
     1741 ccgtgccgtg cgcctgcgcc atccagcggg gcatcgacgg aagatgatcg cgcaccacca
     1801 ggcggttgct cgcgacgttg cgcggatcgt cgccgccgcg cgcgccgaga ttcaaggagg
     1861 cgtactcacc cccgctcacg ccgccatggc gcgttgtgac gaaggcgcgc acgcgggtgg
     1921 gcgcgggcca atcgggcgcg atc
//
LOCUS       DASVII010000527         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_132252,
            whole genome shotgun sequence.
ACCESSION   DASVII010000527 DASVII010000000
VERSION     DASVII010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132252"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF309_06880"
     CDS             <1..184
                     /locus_tag="VF309_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466345.1"
                     /translation="IGGINIDNAALAIEAGADMVAVIHALFGSADVRAAALSFTRLFE
                     HSTRAGPHVRTQPRAI"
     gene            156..1445
                     /gene="hemL"
                     /locus_tag="VF309_06885"
     CDS             156..1445
                     /gene="hemL"
                     /locus_tag="VF309_06885"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX7466346.1"
                     /translation="MYERSRALFDRSQQVIPGGVNSPVRAFRAVGGTPLFIREGRGAR
                     VTDEDGREYIDYVGSWGPLILGHAHPEVVAAVGAAAARGLSFGAPTALELEMAELLTA
                     SLPSMEQVRLVSSGTEAVMSALRLARGYTGRSIILKFEGCYHGHSDSLLVKAGSGALT
                     FGNPTSAGVPAELTSHTLVLDYNDPQQAADAFARHGDGIACVVLEPVAGNMNFVRPAQ
                     EFIDTLRSLCTRHGAVLLFDEVMTGFRVGPRGAQGVFGVEPDLTTLGKVIGGGLPVGA
                     FGGRRDIMQKLAPVGPVYQAGTLSGNPVAVSAGLATLRRVLEPGFFDRLGATTTALVD
                     GLAREARAAGVPFSGGSLGGMFGLFFRAQLPTTFAEVMQSDREAFNRFFHAMLERGVY
                     LAPSAFEAGFVSSAHGAAELEATLGAARAAFASARGR"
     gene            1449..>1659
                     /locus_tag="VF309_06890"
     CDS             1449..>1659
                     /locus_tag="VF309_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466347.1"
                     /translation="MRILPSRARLAGLGLAVAAATIIALAISVLLDLDREAQVHREVA
                     AAQQVKDQLHALRAQVLDLRAAARLG"
BASE COUNT          215 a          589 c          578 g          277 t
ORIGIN      
        1 catcggcggc atcaatatcg acaacgctgc gcttgccatc gaggcgggcg ccgacatggt
       61 ggcggtgatc cacgccctgt tcggctctgc cgatgtgcgt gccgctgcgc tttctttcac
      121 ccgactcttc gaacattcca caagggcagg ccctcatgta cgaacgcagc cgcgcgctat
      181 ttgatcgctc gcagcaggtc attcccggcg gggtcaactc gccggtgcgc gcctttcgcg
      241 ccgtcggggg aacgcctctc ttcattcgcg agggtcgcgg ggcgcgcgtg accgacgagg
      301 atggccgcga gtacatcgac tacgtcggat cctggggtcc gctcatcctc ggccacgcgc
      361 atcccgaggt cgtggccgcg gtgggggcgg ccgcggcgcg cgggctttcc ttcggcgcac
      421 ccaccgcgct cgagctcgag atggccgaac tcctcaccgc gagcctgccg tcgatggagc
      481 aggtgcggct cgtgagctcc ggcaccgaag ccgtgatgag cgcgctgcgc ctcgcgcgcg
      541 gctacacggg ccgctcgatc atcctgaagt tcgaggggtg ctatcacggc cacagcgatt
      601 cgctgctggt gaaagccggg tccggagcgc tcacgttcgg caatccgacc tccgccggag
      661 tgcccgcgga gctcacctcg cacacccttg tcctcgacta caacgaccca cagcaagccg
      721 cggacgcttt cgcgcggcat ggcgatggca tcgcgtgcgt ggtgctcgag cccgtcgcgg
      781 gcaacatgaa cttcgtgcgg cccgcgcagg agttcatcga cacgctgcgc tccctgtgca
      841 cgcgccatgg cgcggtcctt ctgttcgacg aggtgatgac gggctttcgc gtcgggccgc
      901 gcggcgccca gggcgtcttc ggcgtcgagc cggatctcac cacgctcggc aaggtgatcg
      961 gcggcggact gcccgtgggc gcgttcggcg ggcggcgcga catcatgcag aagctcgcgc
     1021 cggtcggccc cgtctaccag gctggaactc tttcgggaaa tccggtcgcc gtgagcgcgg
     1081 ggctcgcgac gctgcgccgc gtcctcgagc cggggttctt cgaccgcctc ggggcgacga
     1141 cgacggcgct cgtcgacggc ctggcgcgcg aggcccgcgc cgcgggcgtg ccgttcagcg
     1201 gcgggagcct cggcgggatg ttcggattgt tctttcgcgc gcagttgccc acgacctttg
     1261 ccgaagtgat gcaaagcgac cgcgaagcgt tcaaccgctt ctttcacgcg atgctggaac
     1321 gcggcgtcta tctcgccccg tcggcattcg aggccggatt cgtgtcctcg gcccatggcg
     1381 cggcggagct cgaggcgacg ctcggcgccg cgcgcgccgc gttcgcgagc gcgcgaggcc
     1441 gctagcccgt gcgcatcctg ccctcacgcg cacgtctcgc gggccttggc ctcgcggtcg
     1501 ccgcggcgac catcatcgcg ctggcgatct cggtcctgct cgacctcgac cgcgaggccc
     1561 aggtgcatcg cgaggtggcg gccgcccagc aagtgaagga tcagctgcac gcgctgcgcg
     1621 cccaggtcct cgacctgcgc gccgcggcga ggctgggtg
//
LOCUS       DASVII010000528         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_167849,
            whole genome shotgun sequence.
ACCESSION   DASVII010000528 DASVII010000000
VERSION     DASVII010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167849"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF309_06895"
     CDS             complement(<1..720)
                     /locus_tag="VF309_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7466348.1"
                     /translation="MSISPRRIALLVAAACAFPLIAGAQSVECAPLPPRPLTLASAEE
                     RLRLCNRDVRASELAAIAAAADRRVAGQRPNPTFTLGASNLNPQAGIGSGNLRDKTFD
                     SSVRIEQLVERGGKAELREAQADRLVDAARADVAEQLREQRLAMRTAFFDLAAAQERV
                     RLQLEFRGLAGETAAASERRFAAGEVSRAEANRFRLDAARAENDARQAQADRERARLD
                     LAKLIGAESSASMLAVEPVWPD"
     gene            801..1481
                     /locus_tag="VF309_06900"
     CDS             801..1481
                     /locus_tag="VF309_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7466349.1"
                     /translation="MRVLIVEDDPTLANGLTETLRREGFVADAVASAAAADAALRVAE
                     IDLVILDIGLPKVDGFTWLKRLRARGGEQAVLILTARDQVTDRVHGLTLGADDYLAKP
                     FVTEELVARVHALARRGRAVRSRLIEHGPLTIDLAKKRALVNGVPLDLSQREHAILEY
                     LFSNIEAIVPKDKIAGAVASWDEHMSANAIEVHISRLRAKLEPAGIRIRTIRGLGYLV
                     EAWSDAKP"
     gene            1519..>2182
                     /locus_tag="VF309_06905"
     CDS             1519..>2182
                     /locus_tag="VF309_06905"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7466350.1"
                     /translation="MVGVAAGWGAYAFLDRRLTAAYDLDLSDIARAIVPHVRMKDGVL
                     TLTLSPDAEAVLRADSTDQIGYAVLDDKGLVVAGDATLPRAPMLPQSYPQFWDDTHDG
                     VGIRAVTIAGVNDGIPVQIVATETRRKREQASRDAALSAIVPTTLLSIAGVFAVLLGV
                     GRGLGPVDDLRRQLQARSHLDLRPVEEVAVANELRPLVHELNEMLARVEGAQSLQARF
                     IAD"
BASE COUNT          324 a          773 c          768 g          317 t
ORIGIN      
        1 atcgggccac accggctcca ctgcgagcat ggacgcggag gattcggcgc cgatcagctt
       61 cgcgagatcg aggcgcgcgc gctcgcgatc ggcctgcgcc tggcgcgcat cgttctcggc
      121 gcgcgccgcg tcgaggcgga agcgattggc ctcggcgcga gagacttcgc ccgcggcgaa
      181 gcggcgctcc gaggccgccg cggtttcccc ggcgaggccg cggaactcga gctgcaggcg
      241 cacgcgctcc tgcgccgcgg cgagatcgaa gaacgcggtg cgcatcgcga ggcgctgctc
      301 gcgcagctgc tccgcgacgt cggcgcgcgc cgcgtcgacc agccgatcgg cttgcgcctc
      361 gcgaagctcg gccttgccgc cgcgctcgac cagctgctcg atgcgcaccg agctgtcgaa
      421 cgtcttgtcg cgcaggtttc ccgagccgat cccggcttgc ggattcaggt tcgacgcgcc
      481 gagcgtgaac gtgggattgg ggcgctgccc cgcgacgcgc cggtcggcgg cggccgcgat
      541 cgccgcgagc tcggaggcgc gcacgtcgcg attgcagagg cgaaggcgct cctcggccga
      601 ggcaagcgtg aggggccgcg gcggcaacgg cgcgcactcg accgactgcg ccccggcgat
      661 gagcgggaaa gcgcacgcgg cggcgacgag gagcgcgatg cgccttggag agatgctcat
      721 gtcgacaggc aacgattggg acgcaaacta tgcatggtca aacttgcatg aatctgtccg
      781 atccgtatga tgttcgtgcc atgcgtgtcc tcatcgtcga agacgacccc accctcgcca
      841 acggattgac cgagacactg cgccgcgaag gcttcgtcgc ggatgcggtg gcgagcgccg
      901 cggcggccga cgctgccttg cgcgtagctg aaatcgacct cgtgatcctc gacatcggcc
      961 tgccgaaagt cgacggtttc acgtggctga agcggctgcg ggcccggggc ggggagcagg
     1021 cggtgctgat cctcacggcg cgcgaccagg tgaccgaccg cgtgcacggc ctcacgctcg
     1081 gcgccgacga ctacctcgcc aagcccttcg tgaccgagga gctggtcgca cgcgtccacg
     1141 ccctggcgcg gcgggggcgc gcggtgcgct cgcgcctgat cgagcacggc cccctcacca
     1201 tcgacctcgc gaagaagcgc gcgctggtga acggcgtgcc gctcgacctt tcgcagcgcg
     1261 agcacgcgat cctcgaatac ctgttctcga acatcgaggc gatcgttccc aaggacaaga
     1321 tcgcgggcgc ggtcgcgtcg tgggacgagc acatgtcggc caacgcgatc gaggtgcaca
     1381 tctcgcgcct gcgcgcgaag ctcgagcccg cgggcatccg catccgcacc atccgcgggc
     1441 tgggctacct ggtcgaagcc tggagcgatg cgaagcctta ggaaacgcct cctcgcgtgg
     1501 ctgcttcttc cgttggtgat ggtcggtgtc gcggcgggct ggggcgccta cgcgttcctc
     1561 gaccggcgcc tcaccgcggc ctacgacctc gacctctcgg acatcgcgcg cgccatcgtg
     1621 ccgcacgtgc ggatgaagga cggcgtgctc acgctcaccc tcagccccga cgccgaagcg
     1681 gtgctgcgcg ccgacagcac cgaccagatc ggctacgcgg tcctcgacga caaggggctc
     1741 gtagtcgccg gcgatgccac cctgcctcgc gcgccgatgc tcccgcagtc ctatccgcaa
     1801 ttctgggacg acacccacga tggggtcgga attcgcgcgg tgacgatcgc cggcgtgaac
     1861 gacggcattc ccgtgcagat cgtcgccacc gagacgcggc gcaagcgcga gcaggcctcg
     1921 cgcgacgcgg cgctctccgc gatcgtgccg acgacgctgc tttccatcgc cggggtgttc
     1981 gcggtccttc tcggcgtggg acgcggcctc ggcccggtgg acgacctgcg ccgccagctc
     2041 caggcgcgat cccatctcga cctgcgtccc gtcgaggagg tcgccgtcgc gaacgagctg
     2101 cgcccgctgg tgcacgagct gaacgagatg ctcgcgcggg tggaaggcgc gcagagcctg
     2161 caggcacgct tcatcgccga cg
//
LOCUS       DASVII010000529         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_167941,
            whole genome shotgun sequence.
ACCESSION   DASVII010000529 DASVII010000000
VERSION     DASVII010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167941"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VF309_06910"
     CDS             complement(<1..654)
                     /locus_tag="VF309_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX7466351.1"
                     /translation="MPFDFVADLSHNALIGVTGDDAAAFLHGQFTNDVESLAPGEAQW
                     NGWCSAKGRLLATFLLLRRADGFLLMLPAEIAAQVSKRLSMFVLRSKVKIQDASPAYA
                     RAGIAGPNVAAWVERHWGSAPKALHAIETGGATCVALDGQRFVVFSPATAAPALRDLF
                     PAEWQRAGADAWEWASIHAGMPTIVAATQEAFVPQMANFELVGGVSFKKGCYPGQEIV
                     "
     gene            664..1116
                     /locus_tag="VF309_06915"
     CDS             664..1116
                     /locus_tag="VF309_06915"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEX7466352.1"
                     /translation="MGAPAREHKYSDATCEISLRPSRSAAWRVAVASLATLALVAAMP
                     GALRLRILFATWIACAALEALHSRALLRGGRAARVVSLAPGGAIAVRNALGRWRAGDL
                     RAGSFVAPWLTVIRWRPDGARFDRTIPILPDMLSQEDFRRVRVMLRWS"
     gene            1141..1638
                     /locus_tag="VF309_06920"
     CDS             1141..1638
                     /locus_tag="VF309_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7466353.1"
                     /translation="MKVLQTERLALRELTVDDAAFIRELVNEPSWIRFIGDRGVRSVD
                     DARSYLEKGPIAMYARFGFGLWAVELKETGEAAGLCGLIKRDALEDVDIGFAFLPRFW
                     RRGYALESAQAVLAYGREILELDRIVAITSPDNDASIRLLEKIGLRFEQTIEFAGGPT
                     KLFAA"
BASE COUNT          262 a          616 c          634 g          282 t
ORIGIN      
        1 gacgatctcc tgccccggat agcagccctt cttgaagctc acgccgccga cgagctcgaa
       61 attcgccatt tgcggaacga aagcctcctg ggtggcggcg acgatggtcg gcatgcctgc
      121 gtggatcgag gcccattccc aggcatcggc gccggcgcgt tgccactcgg ccgggaacag
      181 gtcgcgcaac gcgggcgccg cagtggcggg cgagaagacg acgaagcgtt ggccgtcgag
      241 cgcgacgcac gtggccccgc cggtctcgat cgcgtggagc gccttcggcg cgcttcccca
      301 gtgcctctcg acccaggccg cgacgttcgg gccggcgatt ccggcgcgcg cgtaggccgg
      361 gcttgcatcc tggatcttca ccttcgagcg aagcacgaac atcgagaggc gcttggaaac
      421 ctgcgccgcg atctccgcgg gcagcatgag gaggaacccg tcggcgcgcc gcagcaggag
      481 gaacgtcgcg agcaggcgcc ctttcgcgga gcaccagccg ttccattgcg cctcgcccgg
      541 ggccagcgac tccacgtcgt tcgtgaactg cccatggaga aacgcggccg cgtcgtcccc
      601 cgtcacgccg atgagcgcgt tgtgggagag atcggcgacg aagtcgaacg gcatggagtg
      661 aagatgggag cgccagcgcg ggaacacaag tatagcgacg cgacgtgcga gatctcgctc
      721 aggccctcgc gatcggctgc atggcgggtg gcggtcgcgt cgctcgcgac gctcgccctg
      781 gtcgcggcga tgcccggagc ccttcggctg cggatccttt tcgcgacatg gatcgcgtgc
      841 gccgcgctgg aggcgctcca tagccgcgcg ctgctccggg gcggccgcgc cgcgcgcgtc
      901 gtctcgctgg cgcccggagg cgcaatcgcg gtgcggaacg ccttgggaag atggcgcgcc
      961 ggcgacctgc gcgcgggctc tttcgtcgcg ccgtggctca ccgtcatccg gtggcgcccg
     1021 gacggggcgc gcttcgaccg aaccatcccg atccttcccg acatgctgtc gcaggaggac
     1081 ttccgccgcg tgcgcgtcat gttgcgctgg tcctaggctc cctaggctag gatggcgggc
     1141 gtgaaagtcc tgcaaacaga gcgcctcgcg cttcgcgagc tcaccgtcga cgacgccgcg
     1201 ttcatccgcg agctggtgaa cgagccctcg tggatccgtt tcatcggcga ccgcggcgtg
     1261 cgctcggtcg acgatgcgcg ctcctatctc gagaaggggc cgattgcgat gtacgcccgc
     1321 ttcggcttcg gcctgtgggc ggtcgagctg aaggagacgg gcgaggccgc ggggctgtgc
     1381 ggcctcatca agcgcgacgc gctcgaagac gtggacatcg gcttcgcctt cctgccgcgc
     1441 ttctggcgcc gcggctatgc gctcgagtcg gcgcaggcgg tgctcgccta cggccgcgag
     1501 atcctcgagc tcgaccgcat cgtcgcgatc acgtcgccgg acaacgacgc ctcgatccgc
     1561 ctgctcgaga agatcgggtt gcgcttcgag cagaccatcg agttcgccgg cgggccgacg
     1621 aagctcttcg ccgcatgacg atggcgctgc gcaaggtcgc cgcgactcgc tacgtgacgc
     1681 cgctccgcga ggggggctcc cttcccgcca tcgtggaggc ggacgacgac ggcatgtacg
     1741 ttctcaagtt ccgcggtgcc ggccagggcg agaaagcgct catcgccgag ctca
//
LOCUS       DASVII010000530         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_25052, whole
            genome shotgun sequence.
ACCESSION   DASVII010000530 DASVII010000000
VERSION     DASVII010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25052"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF309_06925"
     CDS             complement(<1..225)
                     /locus_tag="VF309_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7466354.1"
                     /translation="MSTQALFGQLLIGLINGSFYALLSLGLAVIFGLLNIINFTHGAQ
                     YMLGAFAAYFLMQRWGLGYWWALLLAPIAVG"
     gene            complement(293..1510)
                     /locus_tag="VF309_06930"
     CDS             complement(293..1510)
                     /locus_tag="VF309_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466355.1"
                     /translation="MNFKLKTLVAAAAMAFAAAGAQAQISGNVVKIGVLNDMSGLYAD
                     LGGQGSVTAAKMAIEDFKAKEKGLNVEVIFADHQNKADVGSQIASKWIDSEGVDVIVD
                     VPNSGVALAVNQIVRDKGKVFINSGAASSDLTGAKCSPNTVHWTYDTWMLANGTGSAI
                     VKRGGKTWFFLTADYAFGYALERDTEAVVTKSGGTVVGKVRVPINTQDFSSFLLQAQA
                     SKAQIIGLANAGGDTINSIKQAAEFGIVKGGQNLAGLLVFLSDVHGLGLEKAQGLILT
                     TTFYWDMNDQTRAWSKRFAAANGGKYPTMVQAGVYAGVLHYLKAVAALKSDDGTKVVA
                     KMKEMPTDDPLFGKGTIRIDGRKIHPAYLVEVKKPSESKGPYDYYKILATIPAEQAFR
                     PLADGGCELVAKK"
     gene            complement(1539..>1739)
                     /locus_tag="VF309_06935"
     CDS             complement(1539..>1739)
                     /locus_tag="VF309_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466356.1"
                     /translation="IVQALGRIIVKLKERGFTVVLVEQNFRFAAPLADRHYVVEHGKI
                     AESFPASELEAKTAMLHEYLGV"
BASE COUNT          289 a          575 c          535 g          340 t
ORIGIN      
        1 gcccacggcg atcggggcca gcaggagcgc ccaccaatag ccgagccccc atcgctgcat
       61 caggaaatac gccgcgaacg cgccgagcat gtactgcgcg ccgtgggtga agttgatgat
      121 gttgaggagc ccgaagatca ccgcgagccc cagcgaaagc agcgcgtaga acgatccgtt
      181 gatcagtccg atcaggagtt gtccgaagag tgcctgggtg ctcaagacgg tggcctatgc
      241 cggttgggga tgagcggcgc caaaggcgtc cgctccatcc ccgttcccga tcttatttct
      301 tcgcgaccaa ctcgcaccca ccgtccgcga gcgggcggaa cgcctgctcg gccggtatgg
      361 tggcgagaat cttgtagtag tcgtacgggc ccttcgactc ggacggcttc ttcacttcga
      421 cgaggtatgc cgggtggatc ttgcggccgt cgatgcggat cgtgcccttg ccgaacagcg
      481 gatcgtcggt cggcatctcc ttcatcttcg cgaccacttt cgtgccgtcg tcgctcttca
      541 gcgccgcgac cgccttgagg taatgcagca cgcccgcgta gacgccggcc tgcaccatcg
      601 tcgggtactt gccgccgttg gccgccgcga atcgcttgct ccacgcgcga gtctggtcgt
      661 tcatgtccca gtagaaggtc gtcgtgagga tcagcccttg cgccttctcg agcccgaggc
      721 cgtgcacgtc cgaaaggaac accaggaggc cggcgaggtt ctgcccgccc ttcacgatgc
      781 cgaactcggc cgcctgcttg atcgagttga tcgtgtcgcc gccggcgttg gcgaggccga
      841 tgatctgggc cttcgatgcc tgcgcctgca acaggaagga cgagaagtcc tgcgtgttaa
      901 tcggcacgcg caccttgccc acgacggtgc cgcccgattt cgtcaccacc gcctccgtgt
      961 cgcgctcgag cgcgtagccg aaggcgtagt ccgcggtgag gaagaaccag gtcttgccgc
     1021 cgcgcttcac gatcgcgctg ccggtgccgt tggcgagcat ccacgtgtcg taggtccaat
     1081 gcaccgtgtt cggcgagcac ttggcgccgg tgaggtccga agaggccgcg ccggagttga
     1141 tgaacacctt gcccttgtcg cgcacgatct ggttcacggc gagcgcgacg ccggagttgg
     1201 gaacgtcgac gatcacgtcc acgccctcgg aatcgatcca cttgctggcg atctgcgagc
     1261 ccacgtcggc cttgttctgg tggtcggcaa agatcacctc gacgttgagc cccttctcct
     1321 tcgccttgaa atcctcgatc gccatcttcg cggcggtcac cgatccctgg cctccgaggt
     1381 cggcgtacag gcccgacatg tcgttcagca cgccgatctt caccacgttg cccgagatct
     1441 gggcctgggc gccggcggcg gcgaatgcca tcgccgcggc ggcaaccaat gtcttcagct
     1501 tgaaattcat gctcgttgct cctctagttt tcgatgaact acacacccag gtactcgtga
     1561 agcattgccg tcttcgcttc gagttccgac gccgggaagg attcggcgat cttgccgtgt
     1621 tccacgacgt aatgccggtc cgcgagcggc gccgcgaacc gaaaattctg ttccaccagg
     1681 accacggtga agccgcgctc cttcagcttc acgatgatgc gcccgagcgc ctgcacgat
//
LOCUS       DASVII010000531         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_60654, whole
            genome shotgun sequence.
ACCESSION   DASVII010000531 DASVII010000000
VERSION     DASVII010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60654"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..629)
                     /gene="rsfS"
                     /locus_tag="VF309_06940"
     CDS             complement(180..629)
                     /gene="rsfS"
                     /locus_tag="VF309_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7466357.1"
                     /translation="MKKLTTLAKKKLVIAALEDIKAHDIQAIDVRRVTAMFDWIVVAS
                     AESARQTKSLSRHVRDRLRESGASIIGTEGEESGEWVLVDAGDIVAHVMQPAVREYYN
                     LEELWGEGKFDKVVNSTQPVKTVRRKKAAAPRAARKTRKASSPRRGA"
     gene            complement(626..1318)
                     /gene="nadD"
                     /locus_tag="VF309_06945"
     CDS             complement(626..1318)
                     /gene="nadD"
                     /locus_tag="VF309_06945"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX7466358.1"
                     /translation="MAATGLFGGTFDPVHFGHLRLATELAEAFHLEKVIFIPAGLPYH
                     RGRDAHATSEERLTMLKLATARDARFDVDDRELKRGGNTYTYDTLAEIRAERGPGEPL
                     VFLIGTDSFATIDTWHRWTELFDLAHFAVAVRGDDQNWLAKGPGIFPREAWPRLTLNP
                     RDLQAAPAGKVMTFAMTPLAISSTAMRALASESASLRYLTPDPVVEFIRSRNLYAPAR
                     DLREAANPATSR"
     gene            complement(1320..>1623)
                     /locus_tag="VF309_06950"
     CDS             complement(1320..>1623)
                     /locus_tag="VF309_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7466359.1"
                     /translation="VSIKMLPGIDEAIAHIAEYGSQHTDAIVTEDLARAQRFLREVDS
                     SSVVVNASTRLADGYEYGLGAEIGISTNKLHARGPVGLEGLTSQKWIVLGDGHLRH"
BASE COUNT          234 a          552 c          557 g          280 t
ORIGIN      
        1 accccttcat cgtccatcgc cttcgcggcc gcgcgtcccg gcccgcgttc ctcgggcttc
       61 agctcgacca gctccacgcg gatctccggc ggcatgcgcc gcgcgtactc ctggaatcca
      121 tcctggatcc atgccggcat cctgtggccc accgacagga tcgagatgcg catcgcagct
      181 cacgccccgc gccgcgggct cgatgccttg cgagtcttgc gtgccgcgcg cggagccgcg
      241 gctttcttgc ggcgcaccgt cttcacgggc tgcgtggaat tcaccacctt gtcgaacttg
      301 ccctcgcccc acagctcctc gaggttgtag tactcgcgca ccgccggctg catcacgtgc
      361 gccacgatgt cgcccgcgtc gacgagaacc cattcgccgg attcctcgcc ctcggttccg
      421 atgatcgacg cgcccgattc cctgaggcgg tcgcgcacgt ggcgcgacag ggatttggtc
      481 tggcgcgccg actcggcgct cgcgaccacg atccagtcga acatggccgt gacgcggcgc
      541 acgtcgattg cctggatgtc gtgggccttg atgtcctcga gggcggcgat gacgagtttc
      601 ttcttggcga gagtcgtgag cttcttcatc gtgaggtcgc ggggttggcg gcttcgcgca
      661 ggtcgcgtgc cggcgcgtac aggttgcggg agcggatgaa ctccaccacc gggtcgggag
      721 tcagatagcg aagcgaggcc gactcggagg cgagcgcgcg catcgccgtg gaggagatgg
      781 cgagcggcgt catcgcgaag gtcatcacct ttccggcggg cgccgcctgg aggtcgcgcg
      841 gattgagggt gaggcgcggc cacgcctccc tcgggaagat cccgggcccc ttcgcgagcc
      901 agttctggtc gtcgccgcgc accgcgaccg cgaaatgcgc gaggtcaaaa agttcagtcc
      961 agcggtgcca cgtgtcgatc gtcgcgaagg agtcggtgcc gatcaggaac acgaggggct
     1021 cgccggggcc gcgctccgcg cggatctcgg cgagcgtgtc gtacgtgtag gtatttccgc
     1081 cgcgcttcag ctcgcgatcg tcgacgtcga agcgcgcatc gcgcgccgtg gcgagcttca
     1141 gcatcgtgag gcgctcctcg ctcgtcgcgt gcgcatcgcg gccgcggtgg tacggcaggc
     1201 ccgcggggat gaagatcacc ttctcgagat ggaacgcctc ggcgagctcc gtcgcgaggc
     1261 gcaggtgtcc gaaatgaacc ggatcgaagg tgccgccgaa gagtccggtc gcggccaaat
     1321 cagtgacgga gatgcccgtc gcccagcacg atccacttct gcgaggtgag gccctcgagg
     1381 cccaccggcc cgcgcgcgtg cagcttgttg gtggagatcc cgatctccgc gcccagcccg
     1441 tactcgtagc cgtcggcgag gcgcgtcgat gcgttcacca cgaccgagct cgagtcgacc
     1501 tcgcgcagga agcgctgcgc gcgcgccagg tcctcggtca cgatggcatc ggtgtgctgc
     1561 gagccgtact cggcgatgtg cgcgatggcc tcgtcgatcc ccggcagcat cttgatcgag
     1621 acg
//
LOCUS       DASVII010000532         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_420052,
            whole genome shotgun sequence.
ACCESSION   DASVII010000532 DASVII010000000
VERSION     DASVII010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420052"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1907
                     /gene="rpoC"
                     /locus_tag="VF309_06955"
     CDS             <1..>1907
                     /gene="rpoC"
                     /locus_tag="VF309_06955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7466360.1"
                     /translation="EFGDDFSAMMGAEGIRELLRHLDLNREIDQLRKDLDATDSETKI
                     KKIAKRLKVLEAFNRSGIKPDWMVMEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRV
                     INRNNRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADM
                     IKGKSGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKLMALELFKPFIFHKLEIM
                     GLATTIKAAKRMVESQEPVVWDILEDVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKA
                     IQLHPLVCAAFNADFDGDQMAVHVPISLEAQMEARTLMLSSNNVLHTAHGEPVIVPSQ
                     DIVLGLYYATRQRVNARGEGSFFSNIAEVSRAYESQGVELHAAITVRIKESRKNEDGT
                     FTEVVNRYETTVGRALMSEILPAGLSFALINKALKKKEISKLINEAFRRCGIRETVIF
                     ADKLMYTGFTYATRAGLSICVDDLLIPAQKAGIIEESEREVKEIERQYTSGLVTQGER
                     YNKVVDIWGRAGDEVAKAMMKHLKEEEVTDRKGKEVNQESFNSIYMMADSGARGSAAQ
                     IRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSG
                     YLTRRLVDVTQ"
BASE COUNT          388 a          610 c          610 g          299 t
ORIGIN      
        1 gagttcggag acgatttctc ggccatgatg ggtgcagagg gcatccgcga gttgctgcgt
       61 catctcgatc tcaaccgcga gatagaccag ttgcgcaagg acctcgacgc cactgactcg
      121 gaaaccaaga tcaagaagat cgccaagcgc ctgaaggtgc tggaggcgtt caacaggtcg
      181 ggcatcaagc ccgactggat ggtgatggaa gtgctgcccg tgctgccgcc ggacctgcgt
      241 ccgcttgtgc cgctggacgg cgggcgcttc gcgacctccg acctgaacga cctctatcgc
      301 cgcgtgatca accgcaacaa tcgtctcaag cgcctgctcg agctgaaagc gccggaaatc
      361 atcgtgcgca acgagaagcg catgttgcag gaagcggtcg attcgctgct cgacaacggc
      421 cgccgcggca aggcgatgac gggcgccaac aagcgcgcgc tgaagtcgct cgccgacatg
      481 atcaagggca agagcggccg cttccgccag aacctgctcg gcaagcgcgt cgactactcg
      541 ggccgctccg ttatcgtggt gggcccgacg ctgaagctgc accagtgcgg cctgccgaag
      601 ctgatggcgc tcgagctctt caagcccttc atcttccaca agctcgagat catgggcctc
      661 gcgaccacca tcaaggccgc gaagcgcatg gtggaaagcc aggagccggt ggtgtgggac
      721 atcctcgagg acgtgatccg cgagcacccg gtgctgctga accgcgcgcc cacgctccac
      781 cgtctcggca tccaggcctt cgagccggtg ctgatcgagg gcaaggcgat ccagctgcat
      841 ccgctcgtct gcgccgcgtt caacgccgac ttcgacggcg accagatggc ggtccacgtt
      901 cccatctcgc tcgaagcgca gatggaagcg cgcaccctca tgctctcgtc caacaacgtg
      961 ctgcacaccg cgcacggcga gccggtcatc gtgccctcgc aggacatcgt gctgggcctg
     1021 tactacgcga cgcgccagcg cgtgaacgcg cgcggcgagg gctcgttctt ctccaacatc
     1081 gccgaggtga gccgcgccta tgaatcgcaa ggcgtcgagc tgcacgccgc gatcacggtg
     1141 cgcatcaagg agtcgcgcaa gaacgaggac ggcaccttca cggaagtcgt gaaccgctac
     1201 gagacgacgg tgggccgcgc gctcatgtcg gagatcctgc cggcgggcct gtcgttcgcg
     1261 ctcatcaaca aggcgctcaa gaagaaggag atttccaagc tcatcaacga ggcgttccgc
     1321 cgctgcggca tccgcgagac ggtgatcttc gccgacaagc tgatgtacac gggcttcacc
     1381 tacgccaccc gcgcgggcct gtcgatctgc gtcgacgacc tgctgatccc ggcgcagaag
     1441 gccggcatca tcgaggagag cgagcgcgaa gtaaaggaga tcgagcgcca gtacacgtcc
     1501 ggcctcgtga cccagggcga acgctacaac aaggtggtcg acatctgggg ccgtgccggc
     1561 gacgaagttg ccaaggcgat gatgaaacat ttgaaggaag aggaagttac ggatcgcaag
     1621 ggcaaggagg tcaaccagga gtcgttcaac tcgatctaca tgatggcgga ctcgggggcg
     1681 cgcggctccg cggcgcagat ccggcagctc gccggcatgc gcggcctgat ggcgaaaccg
     1741 gatggctcga tcatcgaaac gccgatcacg gcgaacttcc gcgaaggtct gaacgtgctg
     1801 cagtacttca tctcgacgca cggcgcgagg aagggtcttg ccgatacggc attgaagacg
     1861 gccaactccg gctacctcac gcgccgcctc gtcgacgtga cccagga
//
LOCUS       DASVII010000533         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_203946,
            whole genome shotgun sequence.
ACCESSION   DASVII010000533 DASVII010000000
VERSION     DASVII010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203946"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VF309_06960"
     CDS             <1..715
                     /locus_tag="VF309_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7466361.1"
                     /translation="LRFRKPSISAACDPDDLVKPIRILVADDHTLVLAGIRALLEEIE
                     GVSIVGEAHNGRDAVALATSERPDLVVMDISMKELNGIEATMQVRAALPDTQVLILSM
                     HATEDFVRRALKAGASGYIVKDSAPLELKMAIEAIMRGEHYISSRVSKHLVSGLTGGA
                     AQGESSIDSLTPRQREILQMIAEGKSTKEIAFVLNVSGKTVETHRAALMERLGIRDIA
                     GLVLYAVRNHLVSVDGAEE"
     gene            761..1021
                     /locus_tag="VF309_06965"
     CDS             761..1021
                     /locus_tag="VF309_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466362.1"
                     /translation="MLDPEEEVARKLDQVVGKRYVPAARQGVLRRAGSALARWLVGAL
                     CAVAAVATIVWVIESHRMPKDVPRRAEMPVTVTIVPAPAKAP"
     gene            complement(1033..1290)
                     /locus_tag="VF309_06970"
     CDS             complement(1033..1290)
                     /locus_tag="VF309_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466363.1"
                     /translation="MASSHQWVALVDDRQRQSLAHEVQSHHARIVELEETLQDLEANL
                     KQVVDAPEELTLKVERLRMELKGRYAAFSVGTAHLSAYRLA"
     gene            complement(1358..>1839)
                     /locus_tag="VF309_06975"
     CDS             complement(1358..>1839)
                     /locus_tag="VF309_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466364.1"
                     /translation="TVTPVGRVTFSPSSPSNATLAYSVGGFETTTNITRQSWRTDDLT
                     GFFIGARQGVWSGCGPALDGKIDSPATVGVSEQSGQVQIRDVGKGYTCSYSGKHTQTG
                     HVSVMTGTGVCADGVSRFLTATGIQISPIMFSMRYRMDQIGTTCVFEGYVGGVREAS"
BASE COUNT          307 a          597 c          635 g          300 t
ORIGIN      
        1 cctccgtttc aggaagcctt ccatttccgc cgcctgcgac cctgacgacc tcgtgaagcc
       61 cattcgcatc ctcgtcgccg acgaccatac gctggtgctg gcggggattc gcgcgctact
      121 cgaggagatc gagggcgtct cgatcgtcgg ggaggcccac aacggccgcg acgcggtcgc
      181 cctcgcgacc agcgaaaggc ccgatctcgt ggtgatggac atctcgatga aggagctgaa
      241 cggcatcgag gccacgatgc aggtccgcgc ggcccttccg gacacgcagg tgctcatcct
      301 ctcgatgcac gcgacggagg atttcgtgcg ccgcgcgctc aaggcgggag cgtccggcta
      361 catcgtcaag gattccgcgc cgctcgagct gaagatggcg atcgaggcca tcatgcgcgg
      421 cgagcactac atcagctcgc gcgtctcgaa gcacctcgtc tcggggctga cgggcggcgc
      481 cgcgcagggc gaatcgtcga tcgactcgct cacgccgcgc cagcgcgaga tcctgcagat
      541 gatcgcggag ggcaagagca cgaaggagat cgcgttcgtg ctgaacgtga gcggcaagac
      601 cgtggagacg caccgcgcgg ccctcatgga gcgcctgggc atccgcgaca tcgccgggct
      661 cgtgctctac gcggtgcgca atcacctcgt gagcgtcgac ggcgcagaag aatagcgccg
      721 cggctgggat aatgggaaag tcccattcgc accggcactg atgctcgatc ccgaggaaga
      781 agtcgccagg aagctcgacc aggtcgtcgg caagcgctat gtgccggccg cacggcaggg
      841 cgtgctgcgc cgggcgggaa gcgcgcttgc gagatggctg gtgggggcgc tgtgcgcggt
      901 cgcggccgtg gcgacgatcg tctgggtgat cgaatcgcac cgcatgccga aggatgtgcc
      961 gcgtcgcgcc gaaatgcccg tgacggtgac catcgtgccg gcgcccgcca aggcgccttg
     1021 acgagcgtgg cgctaggcga ggcggtacgc ggaaaggtgg gcggtgccga ccgagaaggc
     1081 cgcgtagcgg cccttcaact ccatgcgcag ccgctcgacc ttgagcgtga gctcttcggg
     1141 cgcgtcgacg acctgcttga ggttcgcctc gagatcctgg agggtctcct cgagctcgac
     1201 gatgcgcgcg tggtgggatt gcacctcgtg cgcgagcgat tggcgttgcc ggtcatccac
     1261 tagagccacc cactgatgcg atgacgccat tttcgcgtca ggcgcgcgcg cagtctgtgg
     1321 gaaaagtcac aatgacgccg accgcacatt acgggcgtca cgaggcttcg cgaactcccc
     1381 cgacgtagcc ctcgaagacg cacgtcgtgc cgatctggtc catgcggtaa cgcatcgaga
     1441 acatgatcgg cgagatctgg atcccggtcg cggtgagaaa gcggctcacg ccgtcggcgc
     1501 acacgccggt gcccgtcatc acgctcacgt ggcccgtttg tgtatgtttc cccgagtagc
     1561 tgcaggtata gcccttgccc acgtcgcgga tctgcacctg gcccgattgc tcggagaccc
     1621 cgacggtggc cggggaatcg atcttgccgt cgagcgccgg gccgcacccg ctccagacgc
     1681 cctgccgcgc gccgatgaag aacccggtga ggtcgtcggt gcgccacgat tgccgggtga
     1741 tgttcgtcgt ggtctcgaag ccgcccacgc tgtacgcaag cgtcgcgttg cttgggctcg
     1801 acgggctgaa ggtgacccgt ccgacgggcg tcacggtgc
//
LOCUS       DASVII010000534         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_95939, whole
            genome shotgun sequence.
ACCESSION   DASVII010000534 DASVII010000000
VERSION     DASVII010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95939"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VF309_06980"
     CDS             <1..643
                     /locus_tag="VF309_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7466365.1"
                     /translation="AFLKLSDRWAPPKPDEPDHLYGLLLTFGLALIIEGAFRFQFGSA
                     GLPYAMPEQLIGVTNLGFMYLPIYRAWVIAASLVICFGTWFLIERTKLGAYLRAATEN
                     PQLVQAFGINVPRMITLTYGFGVALAALAGVFAAPIYQVSPQMGSSLIIVVFAVVVIG
                     GMGSIMGSIVTGFALGILEGLTKYFYPEASNTVIFVVMAIVLMIRPQGLFGQR"
     gene            643..1617
                     /locus_tag="VF309_06985"
     CDS             643..1617
                     /locus_tag="VF309_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7466366.1"
                     /translation="MAELESHTAVRDPAWEARYHRGALAVMVVAFAVAPFVMYPQFAM
                     QLLCFALFACAFNLLIGFGGLLSFGHAMFFGMGSYLAAYAAKSWHLPVELAILAGAAT
                     STVLGGLTGLLAIRRQGIYFAMITLALAQMVYFFCVQAPFTGGEDGIQSVPRGKLLGA
                     IDLTQPLAMYYFVFAIFLGGFLLIHRIVHSPFGQVLKAIRENEPRAISLGYRAERYKL
                     AAFVLSAAIAGLAGATKAIVAQNASLTDVYWAMSGEVVLMTLLGGMGTIFGPVVGAVV
                     IIAMQFYLADLGAWVTIAQGIIFVVCVLAFRRGIVGEIEALLRDRRKP"
     gene            1625..>2215
                     /locus_tag="VF309_06990"
     CDS             1625..>2215
                     /locus_tag="VF309_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7466367.1"
                     /translation="MSAPRHPFETGLDRNAANYAPLTPVAFLRRSADVYPGKAAVIHG
                     ESSYSYREFDARCRRFASVLAARGVGRGDTVAIMAPNVPALLEAHYAVPGVGAVLNAL
                     NYRLDAAAIAFCLEHGNAKILIADREFSPVIAQALKLARRETFVVDIDDPLAGSGDLL
                     GDIDYESWIARGDPGFELRNPPDEWDSLALLYTSGTT"
BASE COUNT          301 a          774 c          715 g          425 t
ORIGIN      
        1 ggcgttcctc aagctctccg accgatgggc accccccaag cccgacgagc cggaccatct
       61 gtacgggctg ctgctcacat tcggcctcgc gctcatcatc gagggggcct tccggttcca
      121 gttcggctcc gcggggctgc cgtacgcgat gcccgagcag ctcatcgggg tcaccaacct
      181 ggggttcatg tacctgccca tctatcgggc ctgggtgatc gccgcctccc tcgtcatctg
      241 tttcggcacc tggttcctca tcgagcgcac caagctcgga gcgtacctgc gcgcggctac
      301 cgagaacccg cagctcgtgc aggctttcgg catcaacgtg ccgcgcatga tcacgctcac
      361 ctacggattc ggcgtcgcgc tcgcggcgct ggcgggcgtc ttcgccgcgc ccatctacca
      421 ggtgagcccg cagatgggct cgtccctcat catcgtggtg ttcgccgtgg tggtgatcgg
      481 cggcatgggc tcgatcatgg gctcgatcgt gacgggcttc gcgctcggca tcctcgaggg
      541 actcaccaag tacttctatc ccgaggcctc caacacggtg atcttcgtcg tgatggcgat
      601 cgtcctcatg atccgcccgc aagggctctt cgggcagcgc tgatggccga gctcgagagc
      661 cacaccgccg tgcgcgatcc cgcctgggaa gcgcgctatc accgcggcgc gctggcggtc
      721 atggtcgtgg ccttcgccgt cgcgccgttc gtcatgtatc cgcagttcgc gatgcagctg
      781 ctgtgcttcg cgctcttcgc gtgcgcgttc aatctcctca tcggcttcgg cgggctcctg
      841 tcgttcggcc atgcgatgtt cttcgggatg gggagctacc tggcggcgta cgccgcgaaa
      901 tcatggcacc ttcccgtcga gctcgcgatc ctcgccggcg cggcgacgtc cacggtgctg
      961 ggggggctca cggggctgct cgcgattcgc cggcaaggca tctacttcgc gatgatcacc
     1021 ctcgcgctcg cgcagatggt ttacttcttc tgcgttcagg cgccgttcac cggcggcgag
     1081 gacggcatcc agtcggtgcc gcgcgggaag ctcctcggcg cgatcgacct cacgcagccg
     1141 ctcgcgatgt actacttcgt cttcgcgatc ttcctcgggg gcttcctcct catccaccgc
     1201 atcgtccact cgccgttcgg gcaggtgctg aaggcgatcc gcgagaacga gccgcgcgcg
     1261 atctcgctcg gctaccgcgc cgagcgctac aagctcgccg ccttcgtgct gtcggcggcg
     1321 atcgcggggc tcgcgggagc gaccaaggcc atcgtggcgc agaacgcctc gctcaccgac
     1381 gtgtactggg cgatgtctgg cgaggtggtg ctgatgacgc ttctcggcgg gatgggaacg
     1441 atcttcgggc ccgtcgtcgg cgccgtggtg atcatcgcca tgcagttcta cctggccgac
     1501 ctcggcgcgt gggtgacgat cgcgcaaggc atcatcttcg tggtctgcgt gctcgccttc
     1561 cgccgcggga tcgtgggcga gatcgaggcg ctcctgcgcg accgccgcaa gccataatgg
     1621 gcgggtgagc gcccctcggc accccttcga aaccggcctg gaccgcaacg cggcgaacta
     1681 cgcgccgctc acgccggtcg cgttcctgcg ccgctccgcc gacgtctatc cgggaaaggc
     1741 cgccgtcatt cacggcgaga gctcgtattc gtaccgcgaa ttcgacgcgc gttgccgcag
     1801 gttcgcgtcg gtgctggccg cgcgcggcgt cgggcgtggc gatacggtcg cgatcatggc
     1861 gccgaacgtt cccgcgctcc tcgaggccca ttacgccgtc cccggcgtgg gcgcagtgct
     1921 caatgcgctg aactatcgcc tcgacgcggc ggcgatcgca ttctgcctcg agcatggcaa
     1981 tgcgaagatc ctcatcgccg accgcgagtt ttctcccgtg atcgcgcagg cgttgaagct
     2041 ggctcgccgc gagaccttcg tcgtcgacat cgacgatccc ctcgccggaa gcggcgacct
     2101 gctgggcgac atcgactacg agagctggat cgcgcggggc gaccccggat tcgagcttcg
     2161 aaatccgccg gacgaatggg attccctcgc gcttctctac acgtcgggca cgacg
//
LOCUS       DASVII010000535         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_491875,
            whole genome shotgun sequence.
ACCESSION   DASVII010000535 DASVII010000000
VERSION     DASVII010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491875"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VF309_06995"
     CDS             <1..615
                     /locus_tag="VF309_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7466368.1"
                     /translation="YYQASINRVDPQTGVMERVAQGVRNSVGFDWNPKTKQLWFTNHG
                     RDWVSDEMPNDTLNRVSAKGMNFGYPFCHQGDFLDPDFGKGRTCKEFDAPMAKLGPHI
                     APLGMRFYNGTMFPAEYRGNIFIAMHGSWNKTSKIGYNVTRVVLDDKGSVAKMEPFLE
                     GFLTDPKADPPMWGRPVDVMVMRDGAMLVSDDYNGIIYRVSYKK"
     gene            633..1217
                     /locus_tag="VF309_07000"
     CDS             633..1217
                     /locus_tag="VF309_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7466369.1"
                     /translation="MDAAAHVAAALLSLGVAAPALAVDMEALEKKAVVCAACHGAQGI
                     SANPAIPSLAGQPKQFITTQLVMFREGNRKDAQMSPIAAGMANAEINDYGTYFTTQAP
                     PAPASSSMDPAKVDIAQRLAEQYHCVSCHGPALKGQQHIPRLAGQQAVYLKAQLRGFK
                     ASTRFDMDGNMTSAAQPLTESDIEILAEYLAAFR"
     gene            complement(1241..>1657)
                     /locus_tag="VF309_07005"
     CDS             complement(1241..>1657)
                     /locus_tag="VF309_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7466370.1"
                     /translation="PFSCTNVALLPIFHMAAFVGLGVPTWDIRDAQKGTDMLVRSPYL
                     GEHLAKQLGNHPAVLMRGHGSTVVAENLPRAVGRAVYLDINARMQYQAIMLAGDDKPI
                     VFMDGAEVAANVSWQNYERSWSLWKAKVLARLAEEG"
BASE COUNT          303 a          561 c          515 g          278 t
ORIGIN      
        1 tactaccagg cgtcgatcaa tcgcgtcgat ccgcagacgg gcgtcatgga gagggtcgcg
       61 caaggcgtgc gcaattcggt cggcttcgac tggaacccga agaccaagca gctctggttc
      121 acgaaccacg ggcgcgactg ggtcagcgac gaaatgccga acgacacgct gaacagggtt
      181 tccgccaagg gcatgaattt cggttatccc ttctgccacc agggcgattt cctcgatcct
      241 gatttcggca agggacgcac ctgcaaggag ttcgacgcgc cgatggcgaa gctgggcccg
      301 cacatcgccc cgctgggcat gcgcttctac aacggcacga tgttcccggc cgaataccgc
      361 ggcaacatct tcatcgccat gcacggctcg tggaacaaga ccagcaagat cggctacaac
      421 gtgacgcgcg tcgtcctgga cgacaagggc agcgtcgcga agatggagcc gttcctcgaa
      481 ggcttcctca ccgatccgaa ggccgatccg cccatgtggg gccgccctgt cgacgtgatg
      541 gtgatgcgcg acggggccat gctcgtctcc gacgactaca acgggatcat ctaccgggtc
      601 agctacaaga agtgactcgg ggtttcgcga tcatggacgc ggcggcgcat gtcgccgccg
      661 cgctcctttc cctcggtgtt gcggcgccgg cgctcgcggt cgacatggag gccctcgaga
      721 agaaggccgt cgtgtgcgcc gcgtgccatg gcgcgcaggg catctcggcc aatcccgcca
      781 ttccgtcgct cgccggccag cccaagcagt tcatcacgac gcagctcgtc atgttccgcg
      841 aaggcaatcg caaggacgcg cagatgtcgc cgatcgcggc gggcatggcc aacgcggaga
      901 tcaacgacta cgggacgtac ttcacgacgc aggcgccgcc cgcgcctgca tcgtcctcca
      961 tggatccggc caaggtcgac atcgcccagc gtctcgccga gcagtaccac tgtgtttcgt
     1021 gccacggccc cgccctcaag ggccagcagc acataccgcg cctcgccggg cagcaggccg
     1081 tgtacctcaa ggcgcagctg cgcggcttca aggcatcgac gcgcttcgac atggacggca
     1141 acatgacgtc cgcggcgcag cccctgaccg agagcgatat cgagatcctc gccgagtatc
     1201 tcgccgcttt ccgctgaaaa ttggtctctg acaccagttt tcacccctcc tcagcgagcc
     1261 gcgccagcac cttcgccttc cacaggctcc acgagcgctc gtagttctgc cacgagacat
     1321 tggccgcgac ctcggcgccg tccatgaaga cgatcggctt gtcgtcgccc gccagcatga
     1381 tcgcctggta ttgcatgcgc gcgttgatgt cgaggtagac ggctcgtccc acggcgcgcg
     1441 ggagattctc cgcgacgacc gtcgagccgt gcccgcgcat gagcaccgcg ggatggtttc
     1501 ccagttgttt cgcgagatgc tcgcccaggt acggcgagcg caccagcatg tcggtgcctt
     1561 tctgcgcgtc gcggatgtcc caggtcggaa cgcccagccc cacgaacgcg gccatgtgga
     1621 agatcggcag cagcgcgacg ttggtgcacg agaaggg
//
LOCUS       DASVII010000536         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_276466,
            whole genome shotgun sequence.
ACCESSION   DASVII010000536 DASVII010000000
VERSION     DASVII010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276466"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF309_07010"
     CDS             complement(<1..612)
                     /locus_tag="VF309_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7466371.1"
                     /translation="MLAQTSTQSSSAALENSFAALFEESLARQEMRIGEVITAEVISV
                     DPNVVIVNAGLKSESTIATEEFKNDRGEIEVKPGDFVSVAIEALEDGFGATKLSRDKA
                     KKLAAWTSLDDALANGTIIKGVVSGKVKGGLTVMVNGIRAFLPGSLVDLRPVKDTSHF
                     ENKELEFKVIKLDRKRNNVVVSRRAVMEETAGVERQQLLETLKE"
     gene            complement(732..1406)
                     /gene="cmk"
                     /locus_tag="VF309_07015"
     CDS             complement(732..1406)
                     /gene="cmk"
                     /locus_tag="VF309_07015"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7466372.1"
                     /translation="MSPRSDPPVIAIDGPSASGKGTVAAEVARALGFHYLDSGALYRL
                     VTLAAQRSATPLDDGESLAALASRMNVEFRDGAAWLEGGDVSAVLRTEEVSAGASRVA
                     AQPKVREALLTRQRAFRAPPGLVADGRDMGSVVFPDAPLKVFLTAGVGTRAERRHKQL
                     MEKGMYAKMSDVVEELRRRDERDTSRPVAPLKHYPDAIFLDTTGLTVAEAVRSILGWW
                     REIPRG"
     gene            complement(1396..>1585)
                     /locus_tag="VF309_07020"
     CDS             complement(1396..>1585)
                     /locus_tag="VF309_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466373.1"
                     /translation="LDSAGASQPRAAPVVVDTYDDHRMAMCFSLASFGPAPVRINDPG
                     CVSKTFPGYFAEFARLVS"
BASE COUNT          259 a          513 c          518 g          295 t
ORIGIN      
        1 ttccttcagc gtctcgagaa gctgctggcg ctcgacgccc gcggtctctt ccatcaccgc
       61 gcggcgcgaa accaccacgt tgttgcgctt gcggtcgagc ttgatgacct tgaactcgag
      121 ctccttgttc tcgaagtgcg acgtgtcctt cacgggacgc aggtccacca gcgagccggg
      181 gaggaatgcg cggatgccat tcaccatgac ggtgaggccg cccttcacct tgccgctcac
      241 gacgcccttg atgatggtgc cgttggcgag cgcatcgtcg aggctcgtcc acgcggcgag
      301 cttcttcgcc ttgtcgcgcg agagcttggt cgcgccgaag ccgtcttcga gggcctcgat
      361 ggcgaccgac acgaagtcgc cgggcttcac ttcgatctcg ccgcggtcgt tcttgaattc
      421 ttcggttgcg atggtgcttt cggacttgag tcccgcgttg acgatcacga cgttcgggtc
      481 gaccgagatc acctcggcgg tgatcacttc accgatgcgc atttcctggc gcgcgaggct
      541 ctcttcgaag agcgcggcga agctgttttc gagtgctgct gaggaggatt gggttgaggt
      601 ttgggccaac atgctgcgtg ctttcacagg ttaaacggcg cattgccgtt gttgatcatc
      661 gccaggcggg ccgaagtgcg cttaaccccc tgacaccttt gggcttttgt ttccagcttt
      721 ggacgagatg cctatcctcg cgggatttcc cgccaccagc ccaggatgct gcgcaccgcc
      781 tctgcgacgg tcaggcccgt ggtatcgaga aaaatcgcgt cgggataatg ctttagcggc
      841 gccacggggc gagaggtgtc cctctcgtcg cgacgccgca gctcttccac aacgtcgctc
      901 attttggcat acattccctt ttccatcaac tgcttatggc gccgctcggc ccgggtgccc
      961 acgcccgcgg taagaaacac cttcagaggg gcgtcgggga acaccacgga gcccatgtcg
     1021 cggccgtcgg ccacgagccc cggaggagcg cggaacgcgc gctggcgcgt gagcagggcc
     1081 tcgcgcacct tgggctgcgc ggcgacgcgc gaggcgcccg cgctgacctc ctccgtccgc
     1141 aggacggcac tcacgtcgcc gccttcgagc cacgccgccc cgtcacggaa ctccacgttc
     1201 atgcgcgagg cgagcgcggc gagcgactcc ccgtcgtcca ggggcgtggc gcttcgctgg
     1261 gcagcgagcg tcacgaggcg atagagcgct ccgctgtcga gatagtggaa accgagcgcc
     1321 cgggcgacct cggcggcaac ggtccccttg cctgaggcgg aggggccgtc gatcgcgatg
     1381 accggcggat cggatctagg agacaaggcg cgcgaattca gcgaagtagc cggggaacgt
     1441 cttcgacacg cacccgggat cattgatgcg caccggagcc ggcccgaagg aggcgagcga
     1501 gaagcacatc gccatccgat ggtcgtcgta ggtgtcgaca acgacaggcg cggcccgtgg
     1561 ttgcgaagca ccggcagaat ccaat
//
LOCUS       DASVII010000537         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_420323,
            whole genome shotgun sequence.
ACCESSION   DASVII010000537 DASVII010000000
VERSION     DASVII010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420323"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="rph"
                     /locus_tag="VF309_07025"
     CDS             complement(<1..713)
                     /gene="rph"
                     /locus_tag="VF309_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7466374.1"
                     /translation="MQRSFDRGAGELRPARFHRRYTHHAEGSVLVEMGHTQVLCTASV
                     EEKVPPFLKGKGQGWVTAEYGMLPRATNTRGSREAAVGKQSGRTQEIQRLIGRSLRAV
                     TDLAALGERQITIDCDVLQADGGTRCASITGAMVALADAIAWLRERQSLAQDPVRDFV
                     AAVSVGIVAGEPVLDLDYSEDSGCDTDMNVVMTGAGHFVEIQGTAEKVPFTREQMSRL
                     LDLSSKGISELIALQRKALA"
     gene            complement(720..1625)
                     /locus_tag="VF309_07030"
     CDS             complement(720..1625)
                     /locus_tag="VF309_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX7466375.1"
                     /translation="MRFTIFQDSRTGDRKGNEDRVGYSYSRDVLLMTIADGMGGHIDG
                     EVAAEIAVSEITRRFQQEARNKLKRPVDFLVSSIQSAHRAIVSHAVEHNLLECPRTTV
                     VACIVQNGSAFWAHAGDSRLYVLRRGELVAQTQDHSRVQMMIDAGEITPEMAARHPER
                     NKIFSCLGGVVPPQIATGREFALEPGDTVMLSTDGFWAQIPGNILGGMLRKQTLVDVM
                     PGLLVEAQRRAKGESDNLSVVAMTWENQEDAGVSDTQSMDTEFTSSSNTTEQLESKAP
                     VEDVTDEDIERAINEIQNAIRRVPR"
BASE COUNT          249 a          593 c          629 g          320 t
ORIGIN      
        1 gcaagcgctt tgcgctgaag cgcgatcagc tcggagatgc ccttcgaaga gaggtcgagc
       61 aggcgcgaca tctgctcgcg cgtgaacggg accttctccg cggtgccctg gatctcgacg
      121 aagtggccgg cgccggtcat caccacgttc atgtcggtgt cgcatccgga gtcctcggag
      181 tagtcgagat cgagcaccgg ctcgcccgcg acgatgccca cggagaccgc ggcgacgaag
      241 tcgcgcaccg gatcctgcgc gagcgattgc ctctcgcgca gccaggcgat cgcgtccgcg
      301 agcgccacca tcgcgccggt gatcgaggcg cagcgcgtgc cgccgtcggc ctggagcacg
      361 tcgcagtcga tggtgatctg gcgctcgccc agggccgcga gatccgtcac cgcgcgaagg
      421 ctcctgccga tgaggcgctg gatttcctgg gtgcgtcccg actgctttcc gaccgcggcc
      481 tcgcgcgagc cgcgcgtgtt cgtggcgcgg ggcagcatgc cgtattcggc ggtgacccag
      541 ccctggccct tgcccttgag gaacggcggc actttctctt ccacgcttgc cgtgcagagc
      601 acttgcgtgt gccccatctc gacgaggacc gagccctcgg cgtggtgggt gtagcggcgg
      661 tggaagcgcg cggggcgaag ctcgcccgcg ccgcgatcga atgagcgttg catgaagtcc
      721 tagcggggaa ccctgcggat ggcgttctgg atctcgttga tggcgcgctc gatgtcctcg
      781 tcggtgacgt cctcgacggg ggccttcgat tcgagctgct ccgtcgtgtt ggaggagctg
      841 gtgaactcgg tgtccatgct ctgcgtgtcg gagacgcccg cgtcttcctg gttctcccac
      901 gtcatcgcga ccaccgagag gttgtcgctc tcgcccttgg cgcggcgctg ggcctcgacg
      961 aggaggccgg gcatcacgtc caccagcgtc tgcttgcgca gcatgccgcc gaggatgttg
     1021 ccggggatct gcgcccagaa gccgtcggtc gagagcatca cggtgtcgcc gggctcgagc
     1081 gcgaactcgc ggcccgtcgc gatctgcggc gggaccactc cgccgaggca gctgaagatc
     1141 ttgttgcgct cggggtggcg cgcggccatc tcgggcgtga tctcgcccgc gtcgatcatc
     1201 atctgcacgc gcgagtggtc ctgggtctgg gcgaccaact ctccgcgccg gaggacatag
     1261 agccgcgaat cccccgcgtg cgcccagaac gccgacccgt tctgaacgat gcacgcgacg
     1321 acggtggtgc gcgggcactc gagcaggttg tgctcgaccg cgtgggagac gatcgcgcgg
     1381 tgcgccgact ggatcgagga gacgaggaag tcgacgggcc tcttcagctt gttgcgcgct
     1441 tcctgctgga agcggcgggt gatctcgctc accgcgatct cggcggcgac ctcgccgtcg
     1501 atgtggccgc ccatgccgtc ggcgatcgtc atcagcagga cgtcgcggct gtacgagtag
     1561 ccgacccggt cctcgttgcc cttgcggtcg ccggtgcggc tgtcctggaa gatggtgaac
     1621 cgcatcgccc tattccccta ccgagagaag agcttcgaca gcggcgtgtt gatggtgtcg
     1681 aggaagccgc gcttctcctc gggcggctcc tccatgagcc gcttttgcag gtcgaacgcg
     1741 gtctgcggcc gctgcatgcg gtccaccgcg aggcacgact cgacgatgtc g
//
LOCUS       DASVII010000538         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_600139,
            whole genome shotgun sequence.
ACCESSION   DASVII010000538 DASVII010000000
VERSION     DASVII010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600139"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1542
                     /locus_tag="VF309_07035"
     CDS             23..>1542
                     /locus_tag="VF309_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEX7466376.1"
                     /translation="MDLGIEPGDRVSILSHTRPEWTYACFATLAAGASVVSIYQTNSA
                     EECYYVLHHSGSRVVFVEDAEQLVKVRAVEADLPELEFVIVIEPGDANGRVISLDALR
                     ERGRGRSEDELEGRVGGVTLDAECLLLYTSGTTGPPKACQLTHGNYRAVTSAVETRDM
                     VREDDTVYLFLPLAHALALVVQFLVLDVGATIAYWEKDPQKIVANLMELKPTFFPSVP
                     RIFEKIHALATSGGDAREIEQATRVGVAVRQAQLRGEEVPAKLQAAFERAEQALYVSV
                     RNLFGGCVRQAVTGAAPIAPSILEFFYACGVPVMEGYGMTETASVASVNTLDAFRFGS
                     VGRPLPGVQVRIADGAGIVIKGPNIFAGYYRDAEATRETVVDGWLQTGDLGRLDEDGF
                     LFITGREKDIIITAGGKNISAANLEYTLRHNRWISQAVVLGDGRPYLVALITLDAEEM
                     PAFASEHNLKPEEVHRSESMRAEIQTAIDEVNSHYAPVEQIKRFEILPEDLSQQTG"
BASE COUNT          258 a          469 c          558 g          257 t
ORIGIN      
        1 gacggagttg gcgctcgggc tcgtggacct gggcatcgag ccgggagaca gggtttcgat
       61 cctgtcgcac acgcggccgg agtggacgta cgcgtgtttt gcgactcttg ccgccggcgc
      121 gagtgtggtg tcgatctacc agacgaactc ggcggaggag tgctactacg tgttgcatca
      181 ctccgggtcg cgggtggtgt tcgtggagga cgccgagcaa ctggtgaagg tccgcgcggt
      241 agaggcggat ttgcccgagc tggagttcgt gattgtgatc gagccggggg acgcgaacgg
      301 ccgcgtgatc tcgctcgatg ctctgcgcga gcgggggcga gggcggtcgg aggacgagct
      361 tgaggggcgg gtcggcggcg tcacactgga cgccgagtgc ctgcttctgt acacgtcggg
      421 cacgaccggg ccgcccaagg cgtgccagct cacgcacggc aattatcgcg ctgtcacgtc
      481 tgcggtggag acgcgggaca tggtgcgcga ggacgacacg gtttacttgt tcctgccgct
      541 ggcgcatgcc ctcgcgctgg tcgtccagtt cctggtgctc gatgtgggcg ccacgattgc
      601 ctactgggag aaggacccgc aaaagatcgt ggccaacctg atggagctca aaccgacgtt
      661 cttcccgtcg gtgccgcgga tcttcgagaa gatccatgcg ctggccacgt ccggcggcga
      721 cgcccgagag atcgagcagg cgacgcgtgt gggagtggcg gtgcgccaag cgcagcttcg
      781 cggcgaggag gtccccgcca agctgcaagc ggcgttcgag cgcgccgagc aggccctgta
      841 cgtcagcgtg cgcaacctgt tcggtgggtg cgtgcgccag gcggtgaccg gggcggcgcc
      901 gatcgctccc agcatcctgg agttcttcta cgcctgcggc gtgccggtga tggagggcta
      961 cgggatgacc gagacggcca gcgtggcgag cgtcaatacg cttgatgcgt ttcggtttgg
     1021 gtccgtcggc aggccgctgc cgggcgtcca ggtgcgaatc gccgatggcg ccggcattgt
     1081 gatcaagggc cccaacatct tcgccggcta ctaccgcgac gcggaggcga cgcgcgagac
     1141 agtggtggac ggttggctgc aaaccggcga cctcgggcga ctcgatgagg acggcttcct
     1201 gttcatcacc ggccgcgaga aggacatcat catcacggcc gggggcaaga acatctcggc
     1261 cgcgaacctc gagtacacgc tgcggcacaa ccgctggatc tcgcaggccg tcgtcctcgg
     1321 cgacggccgc ccctacctcg tggcactgat cacgctcgac gccgaggaga tgccggcgtt
     1381 tgcgagtgag cacaacctga agccagagga ggtccatcga tccgagtcga tgcgcgccga
     1441 gatccagaca gcgatcgacg aggtcaactc gcactacgcg cccgtggagc agatcaagcg
     1501 cttcgagatc ctgcccgagg acctctcaca gcaaaccggc ga
//
LOCUS       DASVII010000539         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_492047,
            whole genome shotgun sequence.
ACCESSION   DASVII010000539 DASVII010000000
VERSION     DASVII010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492047"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..286)
                     /locus_tag="VF309_07040"
     CDS             complement(38..286)
                     /locus_tag="VF309_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466377.1"
                     /translation="MSRFMIWSRVEVRHGTFVAVATAIPCKAGEPGVPEERTLECGTR
                     DGAEAAAARLAQSLSDDIHARGGRVPAGSRATEASPLY"
     gene            410..>1568
                     /gene="ccoN"
                     /locus_tag="VF309_07045"
     CDS             410..>1568
                     /gene="ccoN"
                     /locus_tag="VF309_07045"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX7466378.1"
                     /translation="MQAQEMYNYKVVRQFAVMSVVWGVVGMLVGVIIAAELYWPDLSM
                     GIPWLGYGRLRPLHTNAVIFAFGGCALFATSYYVVQRTSQVRIFSDRLAGFTFWGYQL
                     VILLAAITLPLGYTQGKEYAELEWPIDILLAVVWIGYAIVFFGTLAKRKVQHIYVANW
                     FFGAFILTVALLHVVNSAAVPVSFWKSYSAYAGVQDAMVQWWYGHNAVGFFLTAGFLG
                     MMYYFIPKQAGRPIYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVFSLI
                     LLAPSWGGMINGIMTLSGAWHKLRTDPILKFLLVSLSFYGMSTFEGPRMSIKTVNALS
                     HYTDWTIGHVHSGALGWVGFITFGSLYYLIPRLVGRVEMHSVRAISLHFWIA"
BASE COUNT          230 a          513 c          514 g          311 t
ORIGIN      
        1 tcgcgccggc agaccgcgag ccggggcgat ccgcggctca atagaggggc gacgcttcgg
       61 tcgcgcgcga gcctgcgggc acgcgcccgc cgcgagcgtg gatgtcgtcg gaaagggact
      121 gcgcgaggcg cgccgcggcc gcttcggcgc cgtcgcgcgt gccgcactcg agggttcgct
      181 cctcggggac accgggctcc ccggccttgc agggaatcgc cgtcgcgacc gcgacgaagg
      241 ttccatgccg cacctccacc cgcgaccaga tcatgaaccg gctcattcga tcctccgttc
      301 ctggttcgag catcgtcttt tcccggtcgg ggcctttgac cggggtcaaa ggcccgcccg
      361 gcgccttgca tacgatggcg ttgtccggac gaccgcggag ctcgacacga tgcaagcgca
      421 ggaaatgtac aactacaagg tggtgcgcca attcgcggtg atgtccgtgg tgtggggcgt
      481 cgtcggaatg ctggtgggcg tgatcatcgc cgcggagctc tattggccgg atctgagcat
      541 gggcatcccg tggctcggct acgggcgcct tcgcccgttg cacaccaacg cggtgatctt
      601 cgccttcggc gggtgcgcgc tcttcgcgac gtcgtactac gtggtgcagc gcacgtcgca
      661 ggtgcgcatc ttctccgaca ggctcgccgg attcacattc tggggatacc agctcgtgat
      721 cctgctcgcg gccatcacgc tgccgctcgg ctacacccag ggcaaggagt acgccgagct
      781 cgagtggccg atcgacatcc tcctcgcggt ggtgtggatc ggttacgcga tcgtcttctt
      841 cggaacgctc gcgaagcgca aggtccagca catctacgtc gccaactggt tcttcggcgc
      901 gttcatcctc acggtggcgc tgctccacgt cgtgaacagc gccgcggtgc cggtctcctt
      961 ctggaaatcc tattcggcct acgcgggcgt gcaggatgcg atggtgcagt ggtggtacgg
     1021 gcacaacgcg gtgggattct tcctcaccgc gggcttcctg gggatgatgt actacttcat
     1081 tcccaagcag gccgggcgcc cgatctactc gtaccggctc tcggtggtgc atttctgggc
     1141 gctcatcttc acgtacatgt gggcgggccc gcaccatctg cattacacgg cgttgcccga
     1201 ctggacgcag tcgctcggga tggtgttctc gctgatcctc ttggcgccgt cgtggggcgg
     1261 gatgatcaac gggatcatga cgctctcggg cgcgtggcac aaattgcgca ccgacccgat
     1321 cctcaagttc ctcctcgtgt cgctctcgtt ctacggcatg tcgaccttcg agggcccgag
     1381 gatgtcgatc aagacggtga acgcgctgtc gcactacacc gactggacca tcgggcacgt
     1441 gcactcgggc gccctcggct gggtgggatt catcaccttc ggcagcctct actacctcat
     1501 cccgaggctg gtggggcgcg tcgagatgca cagcgtgcgc gcgatctcgc tgcacttctg
     1561 gatcgcga
//
LOCUS       DASVII010000540         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_96158, whole
            genome shotgun sequence.
ACCESSION   DASVII010000540 DASVII010000000
VERSION     DASVII010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96158"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..531)
                     /locus_tag="VF309_07050"
     CDS             complement(34..531)
                     /locus_tag="VF309_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEX7466379.1"
                     /translation="MTQEISSLMDGEAPAQEAESAIRSCSASAEHKETWLIYHAIGDA
                     MRGQSPRVLALPDAVIEALKAQPTVLAPRRRAVDSTFARVAFAAAASVATLGVVGWLG
                     TQGGQGGTSPVIAKSSSSIQAVSNNAPAREIVPDARDYLAAHRQMPSAELYRPVNNRA
                     PAPAR"
     gene            complement(608..1207)
                     /gene="rpoE"
                     /locus_tag="VF309_07055"
     CDS             complement(608..1207)
                     /gene="rpoE"
                     /locus_tag="VF309_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEX7466380.1"
                     /translation="MSDREIDQQLVERAQRGDKHAFEMLVLKYQRKLERLLSRVIRDP
                     TEIEDVAQEAFIKAYRALPNFRGDSAFYTWLYRIGINTAKNYLIALGRRAPTSTEYDS
                     EEAEGFEDAAGLRDINTPESELASKQIAQTVNDAIAALPEELRTAITLRELDGLSYEE
                     IAQIMSCPIGTVRSRIFRAREAVAEKLRPQLGTSKDKRW"
     gene            1437..>2265
                     /locus_tag="VF309_07060"
     CDS             1437..>2265
                     /locus_tag="VF309_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7466381.1"
                     /translation="MSHYDVLILGSGLAGMSAALHLADHCKVALVTKHELLDGASSWA
                     QGGIAAVLDGSDSVEDHIRDTLVAGAGLCNPEAVRFVVERGRHCVEWLVAQGVPFTRE
                     DGNQDELHLTREGGHSHRRIVHAADATGSAVQRTLVQSVKTHPNIEVLEWHIGVDLIT
                     SRKHLGETGEPRVWGAYVLDIKGGRVKTIAARHTVLATGGAGKVYLYTTNPDTATGDG
                     VAIGWRAGAPVSNMEFIQFHPTCLFHPHAKSFLISEAVRGEGGLLRLPDGERFMPWHD
                     "
BASE COUNT          350 a          766 c          736 g          413 t
ORIGIN      
        1 tccaccggcg cccgcccgcc gacaccaggg ctctcatcga gccggggccg gcgctcggtt
       61 gttgaccgga cgatagagct ccgcggaggg catctgccgg tgcgccgcga ggtaatcgcg
      121 cgcgtcggga acgatctcgc gggccggcgc gttgttcgac acggcctgga tcgaggagga
      181 cgacttggcg atcaccggcg aggtgccgcc ctgtccaccc tgcgtgccaa gccatccgac
      241 cacgccgagc gtcgcgaccg acgcggcggc cgcgaaggcc acgcgcgcga aggtcgagtc
      301 gacggcgcgc cgcctgggcg cgagcaccgt gggctgggcc ttgagggcct cgatcaccgc
      361 gtccggaagc gcgagcactc gcggcgactg cccgcgcatc gcgtctccga tggcgtggta
      421 gatcagccat gtttccttgt gctccgcgct cgcactgcac gagcggatcg cgctttcggc
      481 ttcctgggcc ggcgcctcgc cgtccatcag cgaggaaatt tcctgggtca tttcgtgctc
      541 ctgtcgattg gccttcgtct ctcgcgaagg gccatgcttt aaggggttgc agccccggac
      601 ttcgttatta ccagcgttta tccttgctcg ttcccagctg cgggcggagc ttttcggcca
      661 ccgcttcacg cgcccggaag atgcgcgacc ggaccgttcc gatcggacag ctcattatct
      721 gcgcgatctc ctcgtacgag agcccgtcga gctcgcgcaa agtaatcgcg gtgcgcaact
      781 cctccggcaa cgcggcgatc gcatcgttca cggtttgcgc gatctgcttc gacgcgagct
      841 cgctttccgg cgtattgatg tcgcgcagcc cggcggcatc ctcgaagcct tcggcctcct
      901 cgctgtcgta ttcggtcgag gtgggcgcgc ggcgcccgag cgcgatgagg taattcttgg
      961 ccgtgttgat gccgatgcgg tacagccacg tgtagaacgc gctgtcacca cggaaattgg
     1021 gaagcgcccg atacgccttg atgaacgcct cctgggcgac gtcctcgatc tccgtcggat
     1081 cgcgaatcac ccgcgacaga agcctctcga gcttgcgctg atacttgagg acgagcatct
     1141 cgaaagcatg cttgtccccg cgctgcgctc tctcgaccag ctgctggtcg atttccctgt
     1201 cgctcattcg gccttgtatg ggtattttcg tcgtgggggg cagcgctgac aggcccccct
     1261 ctgcggccgg cgccacaaag ggcttcttgg cctcctgggg tattataaag ctcaaagatt
     1321 tcattccttt ggcgcgcatt tcttgattgt ctctgaatga caggggggtc ggtgaatagt
     1381 tcctccccgc gccgggctat ttcccatacg aatgccccta ttctgaaccc tctccgttga
     1441 gccactacga cgtcctgatc ctggggagcg gcctggcagg aatgtcggcc gccctccact
     1501 tggccgacca ctgcaaggtg gccctcgtca ccaagcacga gctcctcgac ggggcctcga
     1561 gctgggcgca ggggggcatc gcggcggtgc tcgacggctc cgactccgtg gaagaccaca
     1621 ttcgcgacac cctcgtggcc ggggcggggc tctgcaatcc cgaggccgtg cgcttcgtgg
     1681 tggagcgcgg gcgccactgc gtcgaatggc tggtcgccca gggcgtgccc ttcacgcgcg
     1741 aggacggcaa ccaggacgag ctgcacctca cccgcgaagg cggccatagc caccggcgca
     1801 tcgtccatgc ggccgatgcc acggggagcg cggtccagag gaccctcgtg caaagcgtga
     1861 agacccatcc caacatcgag gtgctggagt ggcacatcgg cgtcgacctc atcacctcgc
     1921 gcaagcatct cggcgaaacc ggggagccgc gcgtatgggg cgcctatgtc ctcgacatca
     1981 agggcgggcg ggtgaagacg atcgccgcgc gccacacggt gctcgccacg ggcggcgcgg
     2041 gcaaggtgta cctctacacg acgaaccccg acaccgcgac gggcgacggc gtcgcgatcg
     2101 gatggcgggc gggagctccg gtctcgaaca tggagttcat ccagttccat ccgacgtgcc
     2161 tcttccatcc gcacgcgaag tcgttcctca tctcggaagc ggtgcgcggc gaaggcggcc
     2221 tcctcaggct ccccgacggc gagcgcttca tgccgtggca cgacg
//
LOCUS       DASVII010000541         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_25425, whole
            genome shotgun sequence.
ACCESSION   DASVII010000541 DASVII010000000
VERSION     DASVII010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25425"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VF309_07065"
     CDS             <1..319
                     /locus_tag="VF309_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7466382.1"
                     /translation="ATAQTTSAILAGDFNFPPENPAYGEIQRALAGDGPAYRDAWTLV
                     HGKAPHTPTFCVHSTGYSKTPYCCDFLFVSADTARRVRGVEVDSETLYSDHQPVLLDF
                     DDR"
     gene            323..604
                     /locus_tag="VF309_07070"
     CDS             323..604
                     /locus_tag="VF309_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466383.1"
                     /translation="MTPLACPHCKEPVAANPIGRWFARFLCPHCRKALQFDQRTNLIG
                     AAGSACFFIMAWAVLMGGTEEAHVVAWGAGALWLAAMAASYALRRIEKA"
     gene            607..1269
                     /locus_tag="VF309_07075"
     CDS             607..1269
                     /locus_tag="VF309_07075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7466384.1"
                     /translation="MPGRNLELDDRLYDYLLGHSLREHPVQRELREATRGMPHGGMQI
                     GPEQGQLMALLVRMLGARRTIEIGVFTGYSALSVALALPPEGRVVACDVSEEWTAIAK
                     RYWAKAGVAERIDLRIAPALQTLDGLVAAGESGGYDFAFIDADKSNYPAYYERCLALV
                     RKGGLIAVDNTLWSGAVADASKNDKDTADIRAFNDAVHADTRVDLSLLPIGDGLTLAL
                     KR"
     gene            complement(1355..>1577)
                     /locus_tag="VF309_07080"
     CDS             complement(1355..>1577)
                     /locus_tag="VF309_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEX7466385.1"
                     /translation="WRGMLRELGDEAPLWARTLPQIPRLVHRVLSDDAPRRVEIAIRD
                     LARAQVRQTRAMWVIVAVLAVLVAALILR"
BASE COUNT          262 a          548 c          536 g          231 t
ORIGIN      
        1 cgcgacggcg caaacgacga gcgcgattct cgccggcgat ttcaatttcc cgcccgagaa
       61 tcccgcgtac ggcgagatcc agcgcgcgct cgccggagat gggccggcct atcgcgacgc
      121 atggacgctc gtgcacggca aggcgccgca tacgccgacc ttctgcgtgc acagcacggg
      181 ctacagcaag acgccatact gctgcgactt cctcttcgtg agcgccgaca cggcgcgccg
      241 cgtgcgtggc gtggaggtcg attcggaaac gctgtactcg gaccaccagc cggtgctcct
      301 cgacttcgac gatcgctagg ccatgacccc actcgcctgt ccccactgca aggagcccgt
      361 ggccgccaat cccatcggtc gctggttcgc gcgcttcctg tgcccgcatt gccgcaaggc
      421 actgcagttc gaccagcgca ccaacctcat cggcgcggcg ggatcggcgt gcttcttcat
      481 catggcttgg gcggtgctga tgggcgggac agaggaagcg cacgtggtcg cctggggcgc
      541 gggcgcgctg tggctcgcgg cgatggcggc gagctacgcg ctgcgccgca tcgagaaagc
      601 ctagccatgc cgggacgcaa cctggagctc gacgatcggc tgtacgacta cttgctgggg
      661 cactcgctgc gcgagcaccc ggtgcagcgc gagctgcgcg aggcgacgcg cggcatgccg
      721 cacggcggca tgcagatcgg cccggagcag ggccagctca tggcccttct cgtgcgaatg
      781 ctgggcgcgc gtcgcacgat cgaaatcggc gtcttcaccg gctacagcgc gctcagcgtg
      841 gcgctggcgc tgccgcccga agggcgcgtg gtcgcgtgcg acgtgagcga ggagtggacc
      901 gcgattgcga agcgatactg ggcgaaagcg ggcgtggccg aacgcatcga ccttcgcatc
      961 gcgccggcgc tgcaaacgct ggacggcctc gtcgccgcgg gggaatccgg cggctacgat
     1021 ttcgccttca tcgacgccga caagtccaac tatcccgcgt actacgagcg ctgcctggca
     1081 ctcgtgcgca agggcgggct catcgcggtc gacaacacgc tctggtcggg cgcggtcgcc
     1141 gacgcctcga agaacgacaa ggacaccgcc gacatccgtg ccttcaacga cgcggtgcac
     1201 gcggacacgc gcgtcgacct ctcgctgctg cccatcggcg acggcctcac gctcgcgctg
     1261 aagcgataaa aaaaaccggg gacagacctc aattatcacc tgccgacaat tgaggtctgt
     1321 ccccggtggg tgtacccccg gcaaagtttc gcgcctagcg caggatgagg gccgccacca
     1381 ggaccgcgag aacggcaaca attacccaca ttgcgcgggt ctggcgcacc tgcgcccgcg
     1441 ccaggtcgcg gatcgcgatc tcgacgcggc gcggcgcgtc gtcgctcagc acgcggtgca
     1501 cgaggcgcgg gatctgcggc agggtgcgcg cccagagcgg cgcctcgtcg ccgagctcgc
     1561 gcagcatgcc gcgccag
//
LOCUS       DASVII010000542         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_61051, whole
            genome shotgun sequence.
ACCESSION   DASVII010000542 DASVII010000000
VERSION     DASVII010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61051"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /gene="atpD"
                     /locus_tag="VF309_07085"
     CDS             complement(<1..976)
                     /gene="atpD"
                     /locus_tag="VF309_07085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7466386.1"
                     /translation="MSTTQMSEGKIVQCIGAVIDIQFPRDSMPHIYDALTLVDEASGS
                     FAEKGLTFEVEQQLGDGVVRTIAMGSSDGLRRGMRVKNTGAPISVPVGPATLGRVMDV
                     LGRPIDERGPVNADERRSIHAAAPKFDELSPSVELLETGIKVIDLVAPFAKGGKVGLF
                     GGAGVGKTVNMLELINNIAKQHSGLSVFAGVGERTREGNDFYHEMSDAKVIVQEDLSQ
                     SKVAMVFGQMNEPPGNRLRVALTGLTMAEYFRDEGRDVLFFVDNIYRFTLAGTEVSAL
                     LGRMPSAVGYQPTLAEEMGRLQERITSTKKGSITSIQAVYVPADDLTDP"
     gene            complement(1003..1878)
                     /gene="atpG"
                     /locus_tag="VF309_07090"
     CDS             complement(1003..1878)
                     /gene="atpG"
                     /locus_tag="VF309_07090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX7466387.1"
                     /translation="MAGSREIRTKIKSVQNTRKITKAMEMVAASKMRKAQERMRHARP
                     YGDKIRTIAAHLAYANPEYRHPFLIKREGAKDVGAIVITSDKGLCGGLNTNLLRLTLN
                     KAREWQGEGRGVQFCAIGNKGFGFLQRFGGNIVSHLTAIGDTPHMERLVGPVKVMIDA
                     FAEGRIETLYIVYTRFINTMKQEPVIEQLLPLPADRLQENRAATRKNWDYLYEPDARA
                     VLDQLLTRYVEQIIYQSVAENIASEQSARMVAMKSASDNASSVIDDLTLIYNKTRQAA
                     ITKELSEIVGGAAAV"
     gene            complement(1976..>3261)
                     /gene="atpA"
                     /locus_tag="VF309_07095"
     CDS             complement(1976..>3261)
                     /gene="atpA"
                     /locus_tag="VF309_07095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7466388.1"
                     /translation="TVKATGRILEVPVGPELIGRVVNSLGLPIDGKGPINAKMTESIE
                     KVAPGVIARKSVSQPVQTGLKSIDAMVPVGRGQRELIIGDRQTGKTAVAVDTIINQKG
                     KDLICVYVAVGQKASTIANVVRKLEEFGAMAYTIVVAASASDPAAMQYIAPYSGCTMG
                     EYFRDRGQDALIIYDDLTKQAWAYRQISLLLRRPPGREAYPGDVFYLHSRLLERAARV
                     NEEYVEKFTKGAVKGKTGSLTALPVIETQAGDVSAFVPTNVISITDGQIFLETDLFNA
                     GIRPAINAGISVSRVGGAAQTKVIKKLGGGVRLALAQYRELAAFAQFASDLDEATRKQ
                     LERGRMVTELMKQPQYQPLQVWEMALVLFAVNNGYYDDVDVKKALAAERAMRDYVKGK
                     YGELIDRIESTKDLTKDDEGKLHEAIRDFKKNGAY"
BASE COUNT          495 a         1067 c         1048 g          651 t
ORIGIN      
        1 acggatccgt caggtcgtcc gcgggaacgt agaccgcttg aatggaggtg atggagccct
       61 tcttggtcga ggtgatgcgc tcctgcaggc ggcccatttc ctcggccagc gtcggctggt
      121 aaccgaccgc cgacggcatc cggccgagca gcgccgacac ttcggtgccg gccagcgtga
      181 agcggtaaat gttgtcgacg aagaacagca cgtcgcggcc ttcgtcgcgg aagtactcgg
      241 ccatggtgag gccggtgagc gccacgcgca ggcggttgcc cggcggctcg ttcatctggc
      301 cgaacaccat cgcaactttc gactgcgaga ggtcttcctg cacgatcacc ttcgcatccg
      361 acatctcgtg gtagaagtcg ttgccctcgc gggtgcgctc tcccacgccg gcgaacaccg
      421 acaggccgct atgctgcttg gcgatgttgt tgatgagctc gagcatgttc acggtcttgc
      481 cgacgccggc gccgccgaac aggcccacct tgccgccctt cgcgaagggc gccaccaggt
      541 cgatcacctt gattcccgtc tcgaggagct ccaccgaggg cgacagctcg tcgaacttgg
      601 gggcggccgc gtggatcgag cgacgttcgt cggcattcac cggcccgcgc tcgtcgatcg
      661 gccggccgag cacgtccatc acgcgaccga gcgtggcggg gccgacgggc accgagatcg
      721 gggcgccggt gttcttcacc ctcatgccgc ggcgcaggcc gtcggaagag cccatcgcga
      781 tggtgcgcac cacgccgtcg cccagctgct gctcgacctc gaaggtgagg cccttctcgg
      841 cgaacgagcc ggaggcttcg tcgacgagcg tcagcgcgtc gtagatgtgc ggcatgctgt
      901 cgcgcggaaa ctggatgtcg atcaccgccc cgatgcactg gacgatcttg ccttcgctca
      961 tttgagtcgt gctcataggt ctctagtcct aacgaaagat tctcagacgg ctgccgcgcc
     1021 gccgacgatt tccgacaatt ccttggtgat cgccgcctgc cgcgtcttgt tgtaaatgag
     1081 ggtgaggtcg tcgatcacgc tcgatgcgtt gtcgctcgcg ctcttcatcg cgaccatgcg
     1141 cgccgactgc tcgctcgcga tattctccgc aaccgactgg tagatgatct gctccacgta
     1201 acgcgtgagg agctggtcga gcacggcgcg cgcgtcgggc tcgtacaggt agtcccaatt
     1261 cttgcgcgtg gccgcccggt tctcctgcag gcgatcggcc ggaagcggca gcagctgctc
     1321 gatcaccggc tcctgcttca tcgtgttgat gaagcgcgtg tagacgatgt agagcgtctc
     1381 gatcctgccc tcggcgaacg cgtcgatcat gaccttgacc gggccgacga gccgctccat
     1441 gtgcggcgtg tcgccgatcg ccgtgaggtg cgagacgatg ttgccgccga agcgctgcag
     1501 gaatccgaat cccttgttgc cgatggcgca gaactgcacg ccgcggccct cgccttgcca
     1561 ctcgcgggcc ttgttcaggg taagacgcag caagttggtg ttgagccccc cgcacaagcc
     1621 cttgtcggag gtgatgacga tcgcgccgac gtccttcgcg ccctcgcgct tgatgaggaa
     1681 cgggtggcgg tattccgggt tcgcgtacgc caggtgcgcc gcgatggtgc ggatcttgtc
     1741 tccatagggg cgcgcgtggc gcatgcgctc ctgggccttc ctcatcttcg aggcggcgac
     1801 catctccatg gccttggtga tcttgcgcgt gttttgaacg ctcttgatct tggttcgaat
     1861 ttctctggag cccgccatgt ttacctattc gcgctgtctg ccctgtctgc cttctgtatc
     1921 gccgtgttga gcggcacgaa gtgtccgctc caacacgcgc cccgatgatc gaaaatcagt
     1981 acgccccatt tttcttgaag tctctgatcg cctcgtggag cttgccctcg tcgtccttcg
     2041 tcaggtcctt cgtggactcg atccggtcga tgagctcgcc gtacttgccc ttcacgtagt
     2101 cgcgcatcgc gcgctcggcg gcgagcgcct tcttcacgtc gacgtcgtcg tagtagccgt
     2161 tgttcaccgc gaacagcacc agcgccatct cccagacctg cagcggctgg tactgcggct
     2221 gcttcatgag ctcggtcacc atgcggccgc gctcaagctg cttcctcgtg gcctcgtcga
     2281 ggtcggaggc gaattgcgcg aacgcagcga gctcacggta ctgggcgagc gcgagacgca
     2341 cgccgccgcc cagcttcttg atcaccttcg tctgcgcggc gccgcccacg cgcgacaccg
     2401 aaatgcccgc gttgatcgcg gggcggatgc cggcgttgaa gaggtcggtc tcgaggaaga
     2461 tctggccgtc ggtgatcgag atcacgttgg tcggcacgaa cgcggacacg tcgccggcct
     2521 gcgtctcgat gacgggaagc gcggtgagcg atccggtctt gcccttgacc gcgcccttgg
     2581 tgaatttctc gacgtactcc tcgttcacgc gcgcggcgcg ctcgaggagg cggctgtgga
     2641 gatagaacac gtcgccggga taggcttcgc ggcccggcgg gcggcgcagc aggagcgaga
     2701 tctggcgata cgcccacgcc tgcttggtca ggtcgtcgta gatgatgagg gcatcctgcc
     2761 cacgatcgcg gaagtactcg cccatggtgc accccgaata gggcgcgatg tactgcatcg
     2821 ccgcggggtc ggaggccgag gcggcgacga cgatggtgta cgccatcgcg ccgaactcct
     2881 cgagcttgcg caccacgttc gcgatcgtcg aggccttctg cccgacggct acgtagacac
     2941 agatgaggtc cttgcccttc tggttgatga tggtgtccac ggccactgcg gtcttgccgg
     3001 tctggcggtc accgataatc aactcgcgct gtccgcggcc caccggcacc atggcgtcga
     3061 tggacttcag tcccgtctgc accggctgcg ataccgattt gcgcgcgatc actcccgggg
     3121 cgaccttctc gatggactcg gtcatcttgg cgttgatcgg gcccttgccg tcgatcggca
     3181 ggccgaggga attcaccacg cggccgatga gctccggtcc caccggaacc tcgaggatgc
     3241 ggccggtcgc cttcaccgtg t
//
LOCUS       DASVII010000543         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_384672,
            whole genome shotgun sequence.
ACCESSION   DASVII010000543 DASVII010000000
VERSION     DASVII010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384672"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VF309_07100"
     CDS             complement(<1..840)
                     /locus_tag="VF309_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7466389.1"
                     /translation="MSDPLRVACVGMGWWSDVLADAIGRSGKLRIEACYTRSEDKRAK
                     FAAKYGCRAAPAYEEILDDPTIEAIINTTPNATHLETTREAAGAGKHVFLDKPIANTI
                     ADARALTKACRDAKVVLALGYQRRRESHFRWIRAQIDAGRFGKLVNAESNISRDRLGK
                     VDLSSWRYTSEGMPGGVMLQIGIHYTDVLEYLLGPVKAVSGRSAQLVLPGDNPDVASL
                     LLEHENGALSTLNASYASASEYYLMNIYGKEASAYYDLHQGLRYLKRGDTRAEPVACA
                     KNDP"
     gene            complement(833..1315)
                     /locus_tag="VF309_07105"
     CDS             complement(833..1315)
                     /locus_tag="VF309_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7466390.1"
                     /translation="MHYYISGKQEEEEMYVAETKRLTVAGAKKMMVTALSSAQEAGIA
                     ITIAIVDAGGNLMLLERMEGGRFHTVHSATTKAVCAASNRRPTGSKGAQGQPLDVTHA
                     VGLALAAGAERWTAMEGGCPILFAGECVGGIGVAGGSFEFDARVAREAVESIGAKSGE
                     "
     gene            1314..>1591
                     /locus_tag="VF309_07110"
     CDS             1314..>1591
                     /locus_tag="VF309_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7466391.1"
                     /translation="MGLSHIEHFLIAADDIDATRDWYARVLGMTSGPHPDFGFPVHWM
                     YLGDADVVHIGPSAKAANEMQKKYLGRTSQDAGAGTGAIDHIAFRATGL"
BASE COUNT          223 a          537 c          546 g          285 t
ORIGIN      
        1 gggatcgttc tttgcgcatg ccaccggctc ggcgcgcgtg tcgccgcgct tcaagtagcg
       61 cagcccctgg tgcaggtcgt agtacgcgct cgcctccttg ccgtagatgt tcatcaggta
      121 gtactccgag gcggaggcgt agctcgcgtt cagcgtcgag agcgcgccgt tctcgtgctc
      181 gagcaagagg ctcgccacgt cgggattgtc gccgggcagc accagctgcg ccgaacgccc
      241 gctcaccgcc ttcacggggc cgaggagata ctcgagcacg tcggtgtagt ggatgccgat
      301 ctgcagcatc acgccgcccg gcattccctc cgacgtgtag cgccacgacg agaggtccac
      361 ttttcccagg cggtcgcggc tgatgttgga ctcggcgttg acgagcttgc cgaatcttcc
      421 cgcgtcgatc tgcgcgcgga tccagcggaa atggctctcg cggcggcgct ggtagccgag
      481 cgcgagcacg accttggcgt cgcggcacgc cttggtgagc gcgcgcgcat cggcgatggt
      541 gttggcgatc ggcttgtcga ggaagacgtg cttgcccgcg cccgccgctt cgcgcgtcgt
      601 ctcgaggtgc gtggcgttgg gcgtcgtgtt gatgatcgcc tcgatggtcg ggtcgtcgag
      661 gatctcctcg taggcgggcg cggcgcggca gccgtatttc gcggcgaact tcgcgcgctt
      721 gtcctcggag cgggtgtagc acgcctcgat ccggagcttg ccgctgcgcc cgatcgcgtc
      781 cgcgagcacg tcggaccacc agcccatgcc gacgcaggcg acgcgcagcg gatcactcac
      841 cggacttcgc gccgatggat tccaccgcct cgcgcgccac gcgcgcatcg aactcgaagc
      901 tgcctccggc cacgccaatg ccgcccacgc attcgcccgc gaagaggatc gggcagccgc
      961 cttccatcgc ggtccagcgc tcggcgccgg cggcgagcgc gaggcccacg gcatgggtga
     1021 cgtcgagcgg ctggccctgc gcgcccttgg atcccgtggg gcgccggttc gatgcggcgc
     1081 acacggcctt ggtggtcgcc gagtgcaccg tgtggaagcg cccgccctcc atgcgctcga
     1141 ggagcatgag gttgccgccg gcgtcgacga tggcgatggt gatcgcgatg ccggcctcct
     1201 gggcgctgct caaggcggtg accatcatct tcttcgcacc tgccacggtg aggcgcttcg
     1261 tctcggcgac atacatttct tcttcctcct gtttcccgga tatatagtag tgcatgggcc
     1321 tttcgcacat cgagcacttc ctgatcgcgg cggacgatat cgacgccacg cgcgattggt
     1381 acgcccgcgt gctggggatg acctccggtc cgcatcccga cttcggattc ccggtgcact
     1441 ggatgtacct gggcgacgcg gacgtcgtgc acatcggccc gagcgcgaag gcggccaacg
     1501 agatgcagaa gaagtatctc gggcgcacgt cgcaggacgc gggcgcgggg acgggcgcca
     1561 tcgatcacat cgcgtttcgc gccacgggac t
//
LOCUS       DASVII010000544         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_384712,
            whole genome shotgun sequence.
ACCESSION   DASVII010000544 DASVII010000000
VERSION     DASVII010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384712"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VF309_07115"
     CDS             <1..371
                     /locus_tag="VF309_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7466392.1"
                     /translation="KALGVRLSVDDFGTGYSSLSYLKRLPLTALKIDGSFVRDISSGA
                     PDEGLIAKAIISLAHSLHLRVIAEGVETEEQRAFLKAHACDEIQGYLVSRPVAAVECV
                     RFFPATRKAGRKDPLAKPTP"
     gene            complement(368..1354)
                     /locus_tag="VF309_07120"
     CDS             complement(368..1354)
                     /locus_tag="VF309_07120"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7466393.1"
                     /translation="MDLPLVFMFLMGASVLAYVVLDGYDLGVGMLMPAASASEQSTMV
                     ASIGPFWDANETWLVLGIGILLAAFPIAHGVVLGALYLPVVAMLVGLMLRGVAFELRV
                     KAQGWHRDLWNWLFWAGSFIASFCQGVMLGRYITGFERGFGYWAFALMVGASLCGGYV
                     LLGATWLVLKTEGELQRKALGWARWGLLWVALGVGLVSLATPLVSETVRDKWFDFPRT
                     LWLMILPAATVAAGFMVWRWTSAARRGGKGQDAKPFCAAVAIFAIAFLGLAYSIFPFV
                     VIDRLTLWDAAAHPTALKALFLGAAIVLPFIVGYTILSYRIFGGKAKADLYD"
     gene            complement(1359..2714)
                     /locus_tag="VF309_07125"
     CDS             complement(1359..2714)
                     /locus_tag="VF309_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7466394.1"
                     /translation="MEDALVLARAQFGLNIGFHILFPSLTIALAWYLVGFRILYQRTR
                     DPAWLATYKLWLKIFALTFAMGVVTGVTMSFQFGTNWPRYMTTVGAIAGPLLSFEVLT
                     AFFLEATFLGIMLFGMNRVPDWLHITSALVVAFGTIVSAFWILALNSWMQTPAGHVFA
                     DGQWIAADWMAVIFNPSFPYRLAHMLIASGLTASFVVAGLSAWRLLRAPFDTSAATTL
                     RAGITIAAVLIPIQVFVGDQHGLNTLKHQPAKIAAMEALWHTERGAPLVLFAIPDEEK
                     RRNDYSIELPYGASLILTHDAHGEVKGIDAFAPDRPAVAPLFFSFRAMVGLGVLMLVV
                     SWFGLWAMRRGRAAPRWLLWIFAAFTFSGWGATLAGWITTEMGRQPWLVTGVLRTKDA
                     VGEISGAELGASLTGYILTYATLFIAYMIVLTHLAGQGASPDDPSAAAAAVTPLARAA
                     E"
     gene            2775..>3284
                     /locus_tag="VF309_07130"
     CDS             2775..>3284
                     /locus_tag="VF309_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411573.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HEX7466395.1"
                     /translation="MTEPSRPTPTPTPIPPAAPPRPPAVSGDASQRGKLKIFFGAFPG
                     AGKTNAMLTAAQRMRDAGRDVVAGVVDSHESSDRVLLDGFETLPSPAVDGASRAGELD
                     LDAALARHPQVILIDDLAHANPPGSRHPKRWNDADELLARGIDVFTTMSVQHLESLND
                     VVGEITGIRE"
BASE COUNT          613 a         1114 c         1119 g          438 t
ORIGIN      
        1 tgaaggccct cggagtccgc ctctcggtgg acgacttcgg gaccggctac tcgagcctgt
       61 cgtatctcaa gcgcctgccg ctcaccgcgc tcaagatcga cggctcgttc gtgcgcgaca
      121 tctcgagcgg cgctcccgac gagggcctca tcgcgaaggc gatcatctct ctcgcccaca
      181 gcctgcacct gcgcgtgatc gccgagggcg tggaaaccga ggagcagcgc gcgttcctga
      241 aggcgcacgc ctgcgacgag atccagggct acctcgtgag ccgccccgtc gccgcggtcg
      301 agtgcgtgcg cttcttcccg gcgacccgga aggcgggcag gaaggatccg ctcgcgaagc
      361 ccacgcccta gtcgtagagg tcggccttcg ccttgccgcc gaagatgcgg tacgaaagga
      421 tcgtgtagcc gacgatgaac ggcagcacga tcgccgcgcc caggaacaac gccttcagcg
      481 ccgtgggatg agcggcggca tcccagagcg tgagccggtc gatcaccacg aaggggaaga
      541 tgctgtaggc gaggccgagg aacgcgatcg cgaagatcgc gaccgccgcg cagaagggct
      601 tcgcgtcctg gcccttgccg ccgcgccgcg ccgcgctcgt ccagcgccag accatgaatc
      661 ccgccgcgac agtggccgcc ggaaggatca tcagccacag cgtgcgcggg aaatcgaacc
      721 atttgtcgcg caccgtttcg ctcacgaggg gcgtggcgag gctcacgagt cccacgccga
      781 gcgcgaccca cagcagcccc cagcgcgccc acccgagcgc tttcctctgc agctcgccct
      841 cggtcttgag gacgagccag gtcgcgccca ggagcacgta gccgccgcag aggctcgcgc
      901 cgaccatgag cgcgaatgcc caatagccga agccgcgctc gaagcccgtg atgtagcgcc
      961 cgagcatcac gccctggcag aaggacgcga tgaaggagcc cgcccagaag agccagttcc
     1021 acaggtcgcg atgccagccc tgcgccttca cgcgcagctc gaacgccacg ccgcgcagca
     1081 tgaggccgac gagcatcgcg acgaccggaa gatagagagc gcccagcacg acgccgtgcg
     1141 cgatcggaaa tgccgcgagg agaatgccga ttcccagcac gagccacgtc tcgttcgcgt
     1201 cccagaaagg accgatggat gcgaccatgg tcgactgctc cgaagcgctc gcggccggca
     1261 tcagcatgcc gacgccgagg tcgtagccgt cgaggaccac gtaggcgagg accgaggcac
     1321 ccatcaggaa catgaagacg aggggcaggt ccatggcgtt actccgcggc tctcgcgagc
     1381 ggggtcaccg ccgcggcggc ggcggacgga tcgtcgggcg acgcgccctg gcccgcgagg
     1441 tgcgtgagga cgatcatgta ggcgatgaag agcgtggcgt acgtgaggat gtatccggtg
     1501 aggctcgcgc cgagctcggc gcccgagatt tcgcccaccg catccttcgt gcgcagcacg
     1561 cccgtcacga gccacggctg gcgccccatt tccgtcgtga tccatcccgc gagggtcgcg
     1621 ccccatcccg agaacgtgaa cgcggcgaag atccacagca gccaccgggg cgcggcgcgc
     1681 ccgcggcgca tcgcccagag gccgaaccag gacaccacga gcatcagcac gcctaggccc
     1741 accatcgcgc ggaacgagaa gaagagcggc gcgacggcag gccggtcggg cgcgaacgcg
     1801 tcgatgccct tcacctcccc gtgcgcgtcg tgcgtgagga tgaggctcgc gccgtaggga
     1861 agctcgatcg agtagtcgtt gcgccgcttt tcctcgtcgg gaatcgcgaa aaggacgaga
     1921 ggcgccccgc gctcggtgtg ccacagcgct tccatggcgg cgatcttggc aggttggtgc
     1981 ttcagggtat tgaggccatg ctggtccccg acgaacacct ggatcggaat gagcaccgcc
     2041 gcgatggtga tgccggcacg caacgttgtc gccgcgctgg tgtcgaatgg ggcccgcagc
     2101 agccgccacg ccgagaggcc ggcgacgacg aacgaggccg tgaggcccga ggcgatcagc
     2161 atgtgggcga gccgataggg aaaggagggg ttgaagatga ccgccatcca gtcggcggcg
     2221 atccattgac cgtcggcgaa cacgtgtccc gccggggtct gcatccacga gttgagcgcg
     2281 agaatccaga aggcggagac tatcgtcccg aacgccacga cgagggccga cgtgatgtgc
     2341 agccagtcgg gcacgcggtt catcccgaag agcatgatgc cgaggaaggt ggcctcgagg
     2401 aagaaggcgg tgagcacctc gaacgacagg agcggtccgg cgatggctcc cacggtcgtc
     2461 atgtacctcg gccagttggt gccgaactgg aagctcatgg tcacgccggt caccactccc
     2521 atcgcgaagg tgagcgcgaa gatcttgagc cagagcttgt acgtcgcgag ccacgccgga
     2581 tcgcgcgtgc gttggtagag gatgcggaag cccacgaggt accacgcgag cgcgatggtg
     2641 agcgacggga agagaatgtg gaacccgatg ttcaggccga actgggctcg cgctaggacg
     2701 agagcgtctt ccaagggaat ccacggcagc gtcggacttt cgattgtaga ccagtcgcta
     2761 ggctaaaatt gcccatgacc gaaccgagtc gtccgacccc gaccccgacc ccgatccctc
     2821 ccgccgcgcc gccgcgcccg cccgcggtgt cgggcgacgc gtcgcaacgc ggcaagctca
     2881 agatcttctt cggcgccttt cccggcgcgg gcaagaccaa cgcgatgctc accgcggcgc
     2941 aacgcatgcg cgacgcgggc cgcgacgtgg tggccggcgt ggtcgacagc cacgaaagct
     3001 ccgaccgcgt tctgctcgac ggcttcgaga cgctgccgtc acccgcggtc gacggcgcga
     3061 gccgcgcggg cgagctcgat ctcgacgccg cgctcgcgag gcacccccag gtcattctca
     3121 tcgacgacct ggcccacgcg aacccgccgg ggtcgcgtca cccgaagcgc tggaacgatg
     3181 ccgacgagct cctggcgcgc ggcatcgacg tgttcacgac gatgagcgtg cagcacctcg
     3241 agagcctcaa cgacgtcgtg ggcgagatca ccgggatccg cgag
//
LOCUS       DASVII010000545         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_564582,
            whole genome shotgun sequence.
ACCESSION   DASVII010000545 DASVII010000000
VERSION     DASVII010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564582"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VF309_07135"
     CDS             complement(<1..1616)
                     /locus_tag="VF309_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7466396.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAGKEQRKSNIYKG
                     VITRVEPSLEAVFIDYGAERHGFLPFKEIAKQFMVNDGGEFGRSKMATHLRERQELIV
                     QVDKDERGNKGAALTTYISLAGRYLVLMPNNPRGGGVSRRIEGEDRNELREVMDQLVI
                     PDGMSLIARTAGIGRSAEEMQWDLNYLLQLWHAIEKAAEDEKGPCLIYLESSLVVRAI
                     RDLFQPEIGEILVDTQDIYDQAMSFMSTVMPANVDKVKLYSDDIPLFSRFQIEHQIES
                     AYRRDVTLPSGGAIVIDHTEALVSVDVNSARATRGHDIEQTAFNTNVEAADEVARQLR
                     LRDLGGLIVIDFIDMESAKNQREVENRLREALRHDRARVQIGKISRFGLLELSRQRLQ
                     PSLEETTHSPCPRCHGTGYIRDTASTALHVLRILQEEAMKENTAAIHCQVPVDVATYL
                     LNEKRSEIFGIEARLKVNVLLIPNPNLETPNYHVERLRHDATQLDEQKPSYEMVMVPD
                     EEPVGLPTKSPEARPPRPEPLVKGITPDQPA"
     gene            complement(1690..1905)
                     /locus_tag="VF309_07140"
     CDS             complement(1690..1905)
                     /locus_tag="VF309_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466397.1"
                     /translation="MTKNWQPLKGSEAGNRRSPANPSSDGKRINALDAPRISRLCRPS
                     MPADARRRKPEIVPFADYSVMQPNTDF"
     gene            2007..2924
                     /locus_tag="VF309_07145"
     CDS             2007..2924
                     /locus_tag="VF309_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7466398.1"
                     /translation="MRWIEVTEDDAGQRIDNYLLARLKGVPKSRIYRILRSGEVRINS
                     KRVGPEQKVLAGDRIRIPPVRVAERDEDVAAPHFKMPILFEDEALMAIDKPPGIAVHG
                     GSGVAHGVIESLRSMRPQARFLELVHRLDRETSGVLLIAKKRSALTVLHEMMRSRDMD
                     KRYLAGVSGRFRNERQRVRVSLAKHVNSAGEKRVSVSEGGQEAETVLRLVERGPEFSL
                     LEAELLTGRTHQIRVHLAHLGHPVLGDDKYGDFELNKALRKQGLKRMFLHAAKLSFAH
                     PVTGEPLELSSPLPADLEKFRSRIKAEQS"
     gene            2921..>3185
                     /locus_tag="VF309_07150"
     CDS             2921..>3185
                     /locus_tag="VF309_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX7466399.1"
                     /translation="MKYELVIFDWDGTLMDSTGVIAASLQDACRDIGIAVPADRDARY
                     VIGLGLADSFDHVAPSLDEEGRRRLSERYRHHFLAREGEMPLYE"
BASE COUNT          502 a         1015 c         1066 g          602 t
ORIGIN      
        1 gcgggctggt ccggcgtgat gcctttcacg aggggctcgg gacgcggcgg gcgcgcctcg
       61 gggctcttcg tcggcaggcc cacgggttcc tcgtcgggga ccatcaccat ctcgtagctg
      121 ggcttctgct cgtccaactg cgtggcgtcg tggcgcaggc gctcgacgtg gtagttgggc
      181 gtctcgagat tcgggttcgg aatgaggagc acgttcacct tgaggcgcgc ctcgatgccg
      241 aaaatttccg agcgcttctc gttcaggagg tacgtcgcga catcgacggg cacctggcag
      301 tggatggccg ccgtgttctc cttcatcgcc tcttcctgga ggatgcgcag cacgtgcagc
      361 gcggtcgatg cggtatcgcg gatgtagccc gtgccgtggc agcgcgggca gggtgaatgc
      421 gtggtctcct ccagcgaggg ctgcaggcgc tggcgcgaga gctcgagcag gccgaagcgg
      481 gaaatcttcc cgatctgcac gcgcgcgcgg tcgtgccgca acgcctcacg cagccggttc
      541 tcgacctcgc gctggttctt ggcgctctcc atgtcgatga agtcgatcac gatgaggccg
      601 cccaggtcgc gcaggcgcag ctggcgcgcg acttcgtcgg cggcttcgac gttcgtgttg
      661 aacgccgtct gctcgatgtc gtggccgcgc gtggcgcgcg cgctgttcac gtcgacggag
      721 acgagcgctt ccgtgtggtc gatcacgatc gcgccgcccg aaggaagcgt cacgtcgcgg
      781 cggtatgcgc tctcgatctg gtgctcgatc tggaagcgcg agaagagcgg gatgtcgtcg
      841 ctgtacagct tcaccttgtc cacgttggcc ggcatcaccg tgctcatgaa cgacatcgcc
      901 tggtcgtaga tgtcctgcgt gtcgacgagg atctcgccga tctccggctg gaacaggtcg
      961 cggatggcgc gcaccacgag gctcgactcg aggtagatga ggcacgggcc cttctcgtcc
     1021 tcggcggcct tctcgatggc gtgccacagc tgcagcaggt aattcaggtc ccactgcatc
     1081 tcctcggcgc tgcgcccgat gcccgcggtg cgggcgatga ggctcatgcc gtcggggatc
     1141 acgagctggt ccatcacttc gcgaagctcg ttgcggtcct cgccctcgat cctcctcgaa
     1201 acgccgccgc cccgcggatt gttgggcatg aggacgaggt agcgtcccgc gagcgagatg
     1261 taggtcgtga gggccgcgcc cttgttgccg cgctcgtcct tgtcgacctg gacgatgagc
     1321 tcctggcgct cgcgcaggtg cgtggccatc ttcgagcggc cgaactcgcc gccgtcgttg
     1381 accatgaact gcttggcgat ttccttgaac ggaaggaagc catgccgctc ggcgccgtag
     1441 tcgatgaaca cggcctcgag ggaaggttcc acccgggtga tgacaccctt gtagatgttg
     1501 ctcttgcgtt gttctttgcc ggcggactcg atgtcgaggt cgatgagttt ctgaccgtcg
     1561 acaattgcga cccggagttc ctccgcctgg gtcgcgttga aaagcatgcg cttcatcgtt
     1621 actttctccc gcgctctaac acggaagagg gcccgcccgt tccaggggga cccggatcga
     1681 cgagttgctt cagaagtcgg tgtttggttg catcactgag tagtcagcga aagggacgat
     1741 ttctggtttt cttcgtcttg cgtctgcggg cattgaaggc cggcagaggc gtgaaattcg
     1801 tggtgcgtcg agcgcgttga ttcgtttacc atcgctgctt ggattggcag gcgagcgacg
     1861 attgcctgct tcggagcctt tcagcggctg ccagttcttc gtcactatcg acttccgagt
     1921 gacgcgtcga tttcttagaa atcaaccaga tatagtatac gcggatgccg accgaaggca
     1981 aaatagcccc ttccgcggcg cccgcggtgc ggtggatcga ggtcaccgag gacgatgcgg
     2041 ggcagcgcat cgacaactat ctcctcgcgc gcctgaaagg cgtccccaag agccgcatct
     2101 accgcatcct gcgcagcggc gaggtgcgca tcaacagcaa gcgcgtcggg cccgagcaga
     2161 aggtgctcgc cggtgatcgc atccgcatcc cgccggtgcg cgtggccgag cgcgacgagg
     2221 acgtcgccgc gccgcacttc aagatgccga tcctcttcga ggacgaggcc ctcatggcga
     2281 tcgacaagcc cccgggcatc gcggtgcacg gcggcagcgg cgtggcccac ggcgtgatcg
     2341 aatcgctgcg ctcgatgcgc ccccaggcgc gcttcctcga gctggtgcat cgcctcgatc
     2401 gcgagacctc gggcgtgctg ctcatcgcga agaaaaggag cgccctcacg gtgctgcacg
     2461 agatgatgcg cagccgcgac atggacaagc gctacctcgc gggcgtctcc gggcggttcc
     2521 gcaacgagcg ccagcgcgtg cgcgtctcgc tcgcgaagca cgtcaacagc gccggcgaga
     2581 agcgcgtgtc ggtctccgag ggtggccagg aggccgagac cgtgctgcgc ctggtcgagc
     2641 gcgggccgga attcagcctt ctcgaggccg agctcctcac cgggcgcacg caccagatcc
     2701 gcgtgcacct cgcccacctc ggccatccgg tgcttggcga cgacaagtac ggcgacttcg
     2761 agctgaacaa ggcgcttcgc aagcaggggc tgaagcgcat gttcctgcac gccgcgaagc
     2821 tttccttcgc gcacccggtg accggcgagc ccctcgagct ttcgtcgccg ctgcctgcgg
     2881 atctggaaaa attccgctcg cgcatcaagg cggaacagag ttgaagtacg agctggtgat
     2941 cttcgattgg gacggcacgc tgatggactc gacgggagtc atcgccgcga gcctgcagga
     3001 cgcatgccgc gatatcggca tcgccgttcc cgccgatcgc gacgcccgct acgtgatcgg
     3061 cctggggctc gccgactcgt tcgaccacgt ggccccgagc ctcgacgagg aaggccggcg
     3121 caggctctcc gagcgctatc gccatcattt cctcgcgagg gagggcgaga tgcccctcta
     3181 tgaag
//
LOCUS       DASVII010000546         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_61229, whole
            genome shotgun sequence.
ACCESSION   DASVII010000546 DASVII010000000
VERSION     DASVII010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61229"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VF309_07155"
     CDS             complement(<1..1062)
                     /locus_tag="VF309_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEX7466400.1"
                     /translation="MACPPTEKTACPSPPGAVPPITPPPIEYRGKLVAALWLDLPAWG
                     RESLGPALEAFVRSCGVLEKQDAWKGVCAGAQTFAPGSPERDIAAFLERNFDPYQVVN
                     ADESAAGMVTGYYEPLLHGSRTRSQRFRYPLYAVPKDLLVIDLSSVYPDLKHRRLRGR
                     LEGNRVVPYYARGDIDTGATLRGNELVWVDDAVDAFFLHIQGSGQVELENGERMRVGY
                     ADQNGHPFRSLGRLLIERHEIAPERASMQGIKDWAKHNPRKVQDFLNSNPSYVFFREL
                     PGDLPGPIGALGVPLTAERSIAVDPRVVPLGVPVYLATTWPNAQDPLNRLMVAQDTGG
                     AIAGGVRADFFWGFGDAAGS"
     gene            complement(1311..1694)
                     /gene="apaG"
                     /locus_tag="VF309_07160"
     CDS             complement(1311..1694)
                     /gene="apaG"
                     /locus_tag="VF309_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX7466401.1"
                     /translation="MPAAKKYSVAVTAHSTYLPDQSDEEEDRYVFAYTIRITNNGNAT
                     AQLVSRHWIITDADNQVQEVRGMGVVGEQPVLKPGDTFEYSSGSSIPTAVGTMRGSYQ
                     LVAEDGTRFEASIPEFTLSVPRVLH"
     gene            1790..2473
                     /gene="rpe"
                     /locus_tag="VF309_07165"
     CDS             1790..2473
                     /gene="rpe"
                     /locus_tag="VF309_07165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7466402.1"
                     /translation="MAGKDFRIAPSILSADFARLGDEVASVIAAGADLIHFDVMDNHY
                     VPNLTIGPLVCEAIRPMATVPIDVHLMVKPVDRIVPEFAKAGAAIISFHPEASEHVDR
                     TLGLIREHGCKAGLVFNPATPLDHLDYVMDKVDLILIMSVNPGFGGQSFIASALAKLA
                     DARRRIDQSGRDIWLEVDGGVKADNIGAIARAGADTFVAGSAIFGDKDHAGAIRRMRE
                     ELKRATMTP"
BASE COUNT          454 a          885 c          899 g          437 t
ORIGIN      
        1 gctgcccgcg gcgtcgccga atccccagaa gaaatccgcg cgcacgccgc ccgcgatcgc
       61 gccgcccgtg tcctgcgcga ccatcaggcg gttgagcgga tcctgcgcgt tgggccacgt
      121 cgtcgcgaga tacaccggca cgccgagggg cacgacgcgg ggatccaccg cgatcgagcg
      181 ctccgcggta agcggcacgc cgagcgcgcc gatcggtccc ggcaggtcgc ctgggagctc
      241 gcgaaagaag acgtagctcg gattcgagtt gaggaaatcc tgcaccttgc gcgggttgtg
      301 cttcgcccaa tccttgatgc cctgcatcga cgcgcgctct ggcgcgatct cgtggcgctc
      361 gatgagcagg cgcccgagcg agcggaaggg atgcccgttc tggtccgcat agccgacgcg
      421 catgcgctcc ccgttctcga gctcgacctg tcccgagccc tggatgtgca ggaagaaggc
      481 gtccaccgcg tcgtccaccc acaccagctc attgccgcgt agcgtggcgc ccgtgtcgat
      541 gtcgccgcgc gcgtagtagg gcaccacccg gtttccctcg agacgcccgc gaaggcgccg
      601 atgcttgagg tcgggataga ccgatgaaag atcgatgacg agaaggtcct tcggcaccgc
      661 gtaaagcggg tacctgaagc gttgcgagcg cgtgcggctg ccgtgcagga gaggctcgta
      721 gtatccggtg accattcccg cggcggactc gtccgcgttg accacctggt agggatcgaa
      781 gttgcgctcc aagaacgcgg cgatgtcgcg ctcgggcgag ccgggcgcga aggtctgcgc
      841 gccggcgcaa acgcctttcc acgcatcctg cttctcgagc acgccgcagc tgcgcacgaa
      901 cgcctcgagc gcagggccga gactctcgcg gccccacgcg ggcaggtcga gccacaacgc
      961 ggcgacgagc ttgccgcgat attcgatggg cggcggcgtg atcggcggca cggccccggg
     1021 cggcgaaggg cacgccgtct tttcggtggg agggcacgcc aacgattcgg gcgcgcgcga
     1081 cgcttcgcgc acaggcgccg gtgcgcatcc cgcgagcaat cccgcgaatg cgagcgcgca
     1141 gagcgcggcg cgcgatgcaa tcgacgataa tgtccgctcg tgcaatgcaa gcggagaatt
     1201 cattggcctg ggtgttcctc gaaatcgtcg cggcgctcgc gatcgcggtg gggatcgtgt
     1261 ggtggacttt tccgaagaag ccgaaaggcc gcgacgagga acgctgatcc tcaatgcagg
     1321 acgcgcggga cgctcagcgt gaattccgga atggaggcct cgaagcgcgt gccgtcctcg
     1381 gcgaccaact ggtagctgcc gcgcatggtc cccaccgcgg tcggaatgga cgatccgctc
     1441 gaatactcga acgtgtcgcc gggcttcagc accggctgct cgccgacaac gcccatgccg
     1501 cgcacttcct gcacctggtt gtccgcgtcg gtgatgatcc aatggcggct cacgagctgg
     1561 gcggtggcgt tgccgttgtt ggtgatgcgg atcgtgtacg cgaagacgta gcggtcttcc
     1621 tcctcgtcgg actgatcggg aagataggtc gagtgcgccg tcacagcgac gctgtatttc
     1681 tttgctgcgg gcatccgaag atggtagcag agaattccgg gcatccgagg gcgcgcgagg
     1741 gcgattccac gtagaatcgc cacgccttgt cgagccccat tcgagtccca tggccggaaa
     1801 agatttccgc atcgccccga gcatcctttc cgcggatttc gcgcggctgg gcgacgaggt
     1861 tgcgagcgtc atcgcggcgg gcgcggacct catccatttc gacgtgatgg acaaccacta
     1921 cgtcccgaac ctcacgatcg gcccgctcgt ctgcgaggcg atccgtccca tggcgaccgt
     1981 gcccatcgac gtgcacctga tggtgaagcc ggtggaccgc atcgtcccgg agttcgcgaa
     2041 ggccggcgcg gcaatcatca gcttccatcc ggaggccagc gagcacgtcg accgcacgct
     2101 cggcctcatt cgcgagcacg gctgcaaggc agggctcgtc ttcaacccgg cgacgccgct
     2161 cgaccatctc gactacgtga tggacaaggt cgacctcatc ctcatcatgt cggtgaaccc
     2221 cgggttcggc ggccaatcgt tcatcgcctc ggccctggcg aagctcgccg acgctcggcg
     2281 gcggatcgac cagagcgggc gcgacatctg gctcgaggtg gacggcggcg tgaaggcgga
     2341 caacatcggc gccatcgcgc gcgccggcgc cgacaccttc gtcgcgggct cggcgatctt
     2401 cggcgacaag gaccacgccg gcgcaattcg gcggatgcgc gaggaattga agcgggctac
     2461 aatgacgccc tgagtcgcga gactccccta caggcgatcg gatccatggc actgcgcgga
     2521 cttcatttcg aatggcgatg gcacggctgg cgctagcgcg ctcgaccttg cctgcctttt
     2581 cgatctgacg actcgcgtgg tgacacatga ctgaacaaga attcctggcg ctcgccggcg
     2641 ccggctacaa ccggatcccc ctgcagctcg agacg
//
LOCUS       DASVII010000547         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_492298,
            whole genome shotgun sequence.
ACCESSION   DASVII010000547 DASVII010000000
VERSION     DASVII010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492298"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /gene="purF"
                     /locus_tag="VF309_07170"
     CDS             complement(<1..1132)
                     /gene="purF"
                     /locus_tag="VF309_07170"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7466403.1"
                     /translation="MCGIFGVRGSVDAVALTKLGLYSLQHRGQESTGIVAVDEHGAAR
                     GVRSMGLVSEMPVAPFDALRGSMAIGHTRYSTAGSSSIENAQPVLVSSRGGHIALAHN
                     GNLTNAGELRVDLEELGSIFASSMDSEVIVHRLARSTASTPEGRLGEALQGVEGAYSI
                     LIILGETLLAARDPRGWRPLALGRLDGAPVFGSETCALDIVGATYERDIAPGEIFAVD
                     ASGERSIFPLEKKALMRCVFEHVYFARPDSRIFGGSVDRARRELGRRLARDCPVPDAD
                     LVFSVPDSSNSAAIGFAEESGLPYELALIRNHYVGRTFIQPTQATRDAKVRLKYNAVR
                     EVLEGKKVVMVDDSIVRGTTTRGLVALVRGAGAAEVHMRVSSA"
     gene            complement(1136..1639)
                     /locus_tag="VF309_07175"
     CDS             complement(1136..1639)
                     /locus_tag="VF309_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7466404.1"
                     /translation="MQRIHGIVAGAPPRVDLAGAKVLVEALLAAISAGAVRSAHDCAD
                     GGLAVALAESAMMERAHTTGAEIDLSVWERLPLRALLFGEAQGRVIVSTPDQAAVLAI
                     AARHGVTARPIGTVRRSGVLDIRVGTRTIRAPLGRLIDAYHEAIPRIMQRSASAQDVA
                     LISDSVG"
BASE COUNT          268 a          562 c          584 g          227 t
ORIGIN      
        1 gggcggagct cacgcgcatg tgtacctccg ccgcacccgc gccgcgcacg agcgccacca
       61 ggccgcgcgt ggtggtgccg cgcacgatgg agtcgtccac catgacgacc ttcttgccct
      121 cgagcacctc gcgcacggcg ttgtacttga ggcgcacctt ggcgtctcgc gtggcctgcg
      181 tgggctggat gaaggtccgg cccacatagt gattgcggat gagcgcgagc tcgtagggca
      241 atccgctctc ctcggcaaag ccgatggccg cggagttgga ggaatcgggg acgctgaaca
      301 cgaggtcggc gtcgggcacc gggcagtcgc gagcgagacg acggcccaac tctcggcgcg
      361 cgcggtccac cgagccgccg aagatgcggc tgtccgggcg ggcgaagtac acgtgctcga
      421 agacgcaccg catgagcgcc ttcttctcga gcggaaagat cgaccgttca cccgatgcat
      481 cgacggcgaa gatctcgccg ggtgcaatgt cacgctcgta ggtggcgccc acgatgtcca
      541 acgcgcaggt ctccgagccg aacaccggcg cgccgtcgag acggccgagc gcgagcgggc
      601 gccagccgcg cggatcacgc gccgccagca gtgtctcgcc caggatgatg agaatgctgt
      661 acgcgccctc caccccttgc agcgcctcgc cgaggcggcc ttcgggggta gaggcggtgg
      721 agcgcgccag ccggtgcacg atgacctcgg aatccatcga ggaggcgaag atggacccga
      781 gctcctcgag gtcgacgcgc agctcgccgg cgttggtgag gttgccattg tgcgccagcg
      841 cgatgtgccc gccgcgggag ctcacgagca cgggctgcgc gttctcgatg gacgaggagc
      901 cggcggtgct gtatcgcgtg tggccgatgg ccatgctgcc gcgcagcgcg tcgaatgggg
      961 ccaccggcat ctccgacacg agccccatgc tgcggacgcc gcgggccgcg ccatgttcat
     1021 cgacggcgac gatgccggtg gactcctgcc cgcgatgctg cagggaatag agacccagct
     1081 tcgtgagcgc gacggcatcg accgagccgc gaacgccaaa gatgccacac attgatcagc
     1141 ctacggagtc ggagatcagc gcgacgtcct gcgccgaggc gctgcgctgc atgatgcgag
     1201 gaatcgcttc gtgatatgcg tcgatgagcc ggccgagcgg cgcccgaatg gtgcgcgtgc
     1261 ccacccggat atcgagtacg ccggagcggc gaaccgttcc gatggggcgc gccgtgacgc
     1321 cgtgacgcgc cgcaatggcg agcaccgccg cctgatccgg cgtggagacg atcacgcgcc
     1381 cctgtgcttc gccgaagagc agcgcgcgga gcggaagcct ctcccacacc gagagatcga
     1441 tctcggcccc ggtggtgtgc gcgcgctcca tcatcgcgct ctccgcgagc gcgacggcca
     1501 acccgccgtc ggcgcaatcg tgtgcggacc ggacggcgcc cgcgctgatc gcggcgagca
     1561 gcgcctcgac gagcaccttc gcgccggcca agtccacgcg cgggggggcg ccggccacga
     1621 tgccatggat ccgctgcaga t
//
LOCUS       DASVII010000548         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_564706,
            whole genome shotgun sequence.
ACCESSION   DASVII010000548 DASVII010000000
VERSION     DASVII010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564706"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VF309_07180"
     CDS             <1..1342
                     /locus_tag="VF309_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7466405.1"
                     /translation="PDALEYRLSHSGARAVVVDAQALASLEAIRARLPSLAHVIGTAG
                     ARGDGVLDWRETLAKASRHFTPVGTRASDPAAIIYTSGTTGPPKGALLPQCALIGNLP
                     GFEHSHDGFPREGDLFWSPADWAWTGGLWDALMPTLYHGQPILGYRGRFDPGRAFYLL
                     EKYAVRNAFLFPTALKMMMKEHPRPRARFELDLRSMMSGGEALGPAVFHWAREELGVT
                     VNEIFGQTEMNYIVGNSQALWPVRPGSMGRPYPGHRIAVIDEEGREVAPGAVGDVALN
                     RNWIDGTPDPVFFLGYWEDPAATAAKFAGDWCRTGDQAAADADGYLWYHGRADDMIKS
                     AGYRIGPTEIENCLVRHPAVANCAVVPIPDETRGSLIKAFVLLAPGHPASDALKDSIR
                     DHVKRHLAPYQQPREIEFVAELPMTTTGKVQRKVLREREALRRGAATSSDPPTA"
     gene            complement(1459..2358)
                     /locus_tag="VF309_07185"
                     /pseudo
     CDS             complement(1459..2358)
                     /locus_tag="VF309_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
     gene            complement(2365..2754)
                     /locus_tag="VF309_07190"
     CDS             complement(2365..2754)
                     /locus_tag="VF309_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX7466406.1"
                     /translation="MRLLLLVILLGFPVLELVVLVRLGESFGWWILAWVVLAACAGMA
                     LIKEARFAMVARLASGLARGEFSIVALTDSARTVVAGLLLIYPGVVSDLIAITLLLLP
                     GPRFIADSAARRESGGTLIEGDFRRTR"
     gene            2785..3132
                     /gene="cutA"
                     /locus_tag="VF309_07195"
     CDS             2785..3132
                     /gene="cutA"
                     /locus_tag="VF309_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX7466407.1"
                     /translation="MPNVPPEDPVTVIAVLTNLPDSESAFNLARELVRLRLAACVNVL
                     APVTSFYRWEGKDQEATEYPLLVKSTHERFPELAAAIRKRHPYSLPEIVGWPIEHGLA
                     EYLRWVEEETREP"
BASE COUNT          546 a         1060 c         1075 g          491 t
ORIGIN      
        1 ccccgatgcg ctcgagtatc ggctgtccca tagcggcgcg agggcggtgg tcgtggatgc
       61 gcaggcgctc gcgagtctcg aagcgattcg cgcgcgcctt ccttcgctcg cgcacgtgat
      121 cggcacggcc ggggcgaggg gcgatggcgt gctcgactgg cgcgaaacgc tcgcgaaggc
      181 ctcgcgccac ttcacgccgg tcggcacgag ggcgagcgat cccgcggcga tcatctacac
      241 gagcggcacg accggccccc ccaagggcgc gctgctgccg caatgcgcgc tcatcggaaa
      301 ccttcccggc ttcgagcatt cgcacgacgg ctttccccgc gagggcgatc tcttctggtc
      361 gccggccgac tgggcgtgga cgggcgggct gtgggatgcg ttgatgccca cgctctacca
      421 tgggcagccg attctcggct atcgcgggcg cttcgatccg gggcgggcct tctacctgct
      481 cgagaagtac gcggtgcgca acgcgttcct cttcccgacc gcgctcaaga tgatgatgaa
      541 ggagcatccg cggccgcgcg cgcgcttcga gctcgacctg cgctcgatga tgagtggcgg
      601 cgaggcgctc ggaccggcgg tgtttcactg ggcgcgcgag gagctcggcg tcaccgtgaa
      661 cgagatcttc gggcagaccg agatgaacta catcgtcggc aactcccagg cgctgtggcc
      721 ggtgcggccg ggctccatgg gcaggccgta ccccggccat cgcatcgccg tgatcgacga
      781 ggaaggccgc gaggtcgcgc cgggagcggt gggcgacgtg gcgctgaatc gaaactggat
      841 cgacggcacg cccgatccgg tcttcttcct cggctattgg gaagatcccg cggcgaccgc
      901 ggcgaaattc gccggcgact ggtgccgcac gggcgaccag gccgcggccg acgccgatgg
      961 atatctctgg taccacggtc gcgccgacga catgatcaag agcgcgggtt atcggatcgg
     1021 cccgacggag atcgagaatt gcctcgtgcg ccatcccgcg gtcgccaact gcgcggtggt
     1081 gccgattccc gacgagacgc gcggctccct catcaaggca ttcgtgctgc tggcgcccgg
     1141 gcacccggcg agcgacgcgc tgaaggattc catccgggac cacgtgaagc gccatctcgc
     1201 gccgtaccag cagccgcgcg agatcgaatt cgtcgcggag ctgccgatga cgacgaccgg
     1261 gaaggtccag cgaaaggtgc tgcgcgagcg cgaggccctg cgcaggggcg cggctacttc
     1321 ttcggatcca ccgacggcgt gaacgcgtcg cagtagaact cgtcttcggg ggaggctgcg
     1381 ctcctcgacg aaggtcttcc tggcgacgtc ggtcatgccc ggcgcgccgc acgcgtacac
     1441 ctggtagccc gacaggtcgg cgaagtcatc gagcacggcc tggtgcacga gcccggtgcg
     1501 tccctgccat cgatcctcgg gcagcggatc ggaaagaacc gggatgtagg tgaaattcgg
     1561 atggtcgcgc tgccattgga tcggcaggcc gggcatgtag aggtcgcgca gcgaccgcac
     1621 gccccagtag aggacgatgg gcaggtcgtc gccgtggctg aaagcgtgct cgatgatcgc
     1681 cttgatcggc gcgaatccgg tgccgcccgc gacgaagatc atcggcttgt ccgaatcctc
     1741 gcgaaggtag aaggtgccga gggggccctc gaagcgcagg atggcgcgct ccttcatctc
     1801 gtcgaacaca tagctcgaga acgcgccgcc cggggccttg cgaacgtgca gctcgatcag
     1861 ctggtcgtcg tggggcgcat tggccatcga gaagctgcgc cgcttgccgc tcttcagcag
     1921 gatgtcgatg tattggcccg cgagaaactg caggcgctcc ccggttggca gcttgagata
     1981 gaggaccgcc acgtcttcgg cgggccgctc caccttctcc acgcgcgcgg gcatcgtgcg
     2041 gatcgcgagg tcccgggcgc cgctcagctc gcgcacctcg aacacgacat cggtcagcgg
     2101 atgcgtgcag caggtgagcg ccttcccggc gatcttctcg tggtcgtgga gcgcgcgctc
     2161 ctggtactcg tcgtaggcga gctctccgga gcgcagcacc cccttgcacg ctccgcaggc
     2221 cccgttgcgg cagccatagg gcaggccgac gccctggcgc agtgccgcgg cgaggatggt
     2281 ctcgcccggc tcgacacgga acgcgtgacc actcggctcg aggcgaacct cgtggccgga
     2341 gtttggagaa tcaggcaatg gtcttcagcg agtccggcgg aaatcgcctt cgatgagagt
     2401 gcccccggat tcgcggcgcg cggccgaatc ggcgatgaag cgagggcccg gaagaaggag
     2461 aagcgtgatc gcgatgaggt ccgagacgac cccggggtag atgagcagga gccccgcgac
     2521 cacggtccgc gcgctgtcgg tcaatgccac gatcgagaac tctccccggg caaggcccga
     2581 ggcgaggcgc gcgaccatcg cgaaacgcgc ctccttgatg agcgccatgc cggcacacgc
     2641 ggcgaggacg acccacgcga ggatccacca cccgaaggat tcgccgagcc gcaccagcac
     2701 cacgagttcc agcacgggga aacccagtaa aatcaccagc agtaagagcc gcatcgcctt
     2761 gcaccttctt ccatcgccga atcaatgccg aacgtcccgc ccgaggaccc agtgaccgtc
     2821 atcgcggtgc tcaccaacct acccgactcc gagtccgctt tcaatctcgc ccgcgagctg
     2881 gtccgcctgc gccttgccgc ctgcgtgaac gtcctggcgc cggtgacttc gttctatcga
     2941 tgggagggca aggaccagga agccaccgaa tatccgctcc tcgtaaagtc cacacacgaa
     3001 cgatttcccg agctcgccgc ggcgatccgc aagcgacatc cctattcgct tccggagatc
     3061 gtcggttggc cgatcgagca cggcctcgca gaatacctgc gctgggtcga agaggaaacc
     3121 cgtgaaccgt gagcgtgcgg ccctgcaagt aatcccgcaa gcgaccgaaa ct
//
LOCUS       DASVII010000549         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_168671,
            whole genome shotgun sequence.
ACCESSION   DASVII010000549 DASVII010000000
VERSION     DASVII010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168671"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..975)
                     /locus_tag="VF309_07200"
     CDS             complement(130..975)
                     /locus_tag="VF309_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEX7466408.1"
                     /translation="MSVVAMTQEMATLGKDVALGVAEKLGLQLVRHEVGDMVAGRMQV
                     KKSLIRRIREGKASRIEKWSADEKTISIFTAEEVFDLAVKGNVLIRGWGATLLLRSVP
                     HIACIRVCAPLELRVKRLMERLETDDEDLARREIEVDDAARAGRMGEHFNVTWGDPTL
                     YDLTLNTERVSIPACVDEVVSLVQSRDFQETAQSRQLLADLALQARALAALRADPRSA
                     GIDIAVEVKDGRIKARGIVVDDREKALVKEVLQPLPGAKGVDDELRTMAGGLYRFPAH
                     VKDKG"
     gene            complement(980..>1731)
                     /locus_tag="VF309_07205"
     CDS             complement(980..>1731)
                     /locus_tag="VF309_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7466409.1"
                     /translation="RAAAVGCFMGIVPGGATPASFMSYGIAKKMSRDGAKFGTGQLEG
                     VVAPETAAHAAGTSALLPMLTLGVPGSPTAAVLLGGLLIWGLQPGPMLFAEKPDFVWG
                     LIASMYLGNIAGLIVVLTCVPLFAAILRVPFAIIAPVIMVICAIGAYTVHSSMFDVYQ
                     MVVFGVMGYVFKNLDYPLAPLVLALVLGDRAEDSFRQSMLASQGDMRILYSNSLVGSI
                     TTLALLLLFWPLISLGLEMIRGKRSTASVVD"
BASE COUNT          302 a          601 c          568 g          260 t
ORIGIN      
        1 agaagaagcg caaggaattc gagcgctatc gtaagaagcg agatccctcc ccgtcgagaa
       61 gccgtggccc cccggctcga gaagaaccgc cccttccacg cccggccgat caccggggtg
      121 ttgaacgggt cagcccttgt ccttcacgtg cgcgggaaag cggtagagcc ccccggccat
      181 ggttcgaagc tcgtcgtcca cgcccttggc ccccggcagc ggctgcagga cctccttcac
      241 gagggccttc tcgcgatcgt cgacgacgat gccgcgtgcc ttgatgcgac cgtccttcac
      301 ctcgaccgcg atgtcgattc cggcggagcg cggatcggcc ctgagcgccg cgagggccct
      361 cgcctggagc gcgaggtcgg cgagaagctg gcgcgattgc gccgtctcct ggaagtcgcg
      421 gctctgcacg aggctcacca cctcgtcgac gcaggcgggg atcgagacgc gctcggtgtt
      481 gagcgtgagg tcgtagagcg tcggatcgcc ccacgtcacg ttgaaatgct cgcccatgcg
      541 ccccgcgcgc gcggcgtcgt cgacctcgat ctcgcggcgc gcgagatcct cgtcgtcggt
      601 ctcgaggcgc tccatgaggc gcttcacgcg caattccagc ggcgcgcaca cgcggatgca
      661 ggcgatgtgc ggcacggagc gcagcagcag cgtcgcgccc catccgcgga tgagcacgtt
      721 gcctttcacc gcgaggtcga acacttcctc ggccgtgaag atcgagatgg tcttctcgtc
      781 ggcggaccac ttctcgatgc ggctcgcctt gccctcgcgg atgcgccgga tcaggctctt
      841 cttcacctgc atgcggcccg cgaccatgtc gccgacctcg tggcggacaa gctgcaggcc
      901 gagcttctcc gcgaccccca gcgcgacgtc cttgccgagg gtggccatct cctgagtcat
      961 cgcgacgacg ctcatggcat cagtccacga ccgaagcggt cgaacgcttc ccgcgaatca
     1021 tttcgagccc cagcgagatc agcggccaga aaaggagcag cagggccagg gtcgtgatcg
     1081 aacccaccag cgagttggag tacaggatgc gcatgtcccc ctgggatgcc agcatcgatt
     1141 ggcggaagga atcctcggcg cggtcgccca gcaccagcgc cagcaccagc ggcgcgagcg
     1201 ggtagtccag attcttgaag acgtagccca tcacgccgaa gacgaccatc tggtacacgt
     1261 cgaacatgga gctgtgcacc gtgtacgcgc cgatcgcgca gatcaccatg atcaccggcg
     1321 cgatgatcgc gaatggaacg cgcaggatcg cggcgaaaag cggcacgcag gtgagcacca
     1381 cgatgagccc cgcgatgttg ccgaggtaca tgctcgcgat caggccccag acgaagtctg
     1441 gcttctccgc aaagagcatg gggcccggct gcaggcccca gatgagcagc ccgccgagga
     1501 gcaccgccgc ggtgggagac ccgggcacgc ccagcgtgag catcggcagc agcgcgctcg
     1561 tgcccgcggc gtgcgcggcg gtctcgggcg cgaccacgcc ctcgagctgc ccggtgccga
     1621 acttggcgcc atccctcgac atcttcttgg cgatgccgta gctcatgaac gaggcgggag
     1681 tcgcgcctcc cggcacgatg cccatgaagc agccgaccgc ggcggcgcgc a
//
LOCUS       DASVII010000550         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_708781,
            whole genome shotgun sequence.
ACCESSION   DASVII010000550 DASVII010000000
VERSION     DASVII010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708781"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VF309_07210"
     CDS             <1..1401
                     /locus_tag="VF309_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466410.1"
                     /translation="CDGDPRATPESLAAKASTYDARAIALHTTQATPWILDVQLASAV
                     DPEVATRADVVAWRSGENDGLWSALVLAAEAYRYAVTHEPAARDALATLLHGEQLRMQ
                     ITGVPGLFTRQLIPPGIAGLACPSDPALYVPTADKRGNRWVRIDDAGCADTADGGGAF
                     HATSHCGLAQFAGWCFLDNVSQDEYAGHLFALGAVARLVDDAELHAIAVDLLDQIGTH
                     LVANHMSFIDWDGRPTQYGKLFPDGGGDTPGYLAVLGASALAITARGTGDPALADAYA
                     ALAPVNATYLDQIMVWSGSDGCTSNWNDISMLTAAFHLLLWNDSAASRAPFLAAFERE
                     LVTQPRGITVEHNAWYDLMWAMQKPLGPGTDGPAYAVVEDAVCQLREFPRSNHVVAHD
                     TTALAAEACTGRQGESLAMSAFATADRCAATYLWWGDPYVRASCTTDPTLVQNPGGYL
                     LPYWMARYAGFVAAAQ"
BASE COUNT          248 a          569 c          532 g          244 t
ORIGIN      
        1 tgtgatggcg atccgcgcgc gacacccgaa tcactcgcgg ccaaggcgag cacgtacgac
       61 gcgcgcgcga tcgcgctcca caccacgcag gcgacgccgt ggatcctcga cgtccagctc
      121 gcgtcagctg tcgatcccga agtcgcgacg cgcgccgatg tcgtcgcgtg gcgcagcggc
      181 gagaacgacg ggctgtggag cgcactcgtg ctagcggccg aggcgtatcg ttacgcggtc
      241 acccacgagc ccgccgcgcg cgacgcactc gcgacgctgc tccacggcga gcaactcaga
      301 atgcagatca ccggcgtgcc cgggttgttc acgcgccagt tgatccctcc cggcatcgcg
      361 ggcctcgcgt gcccgagcga tcccgcactg tacgtgccga ccgccgacaa acgcggcaac
      421 cggtgggtcc ggatcgacga cgccggctgc gccgacaccg cggatggcgg cggagcgttc
      481 cacgcgacca gccattgcgg cctcgcacag ttcgccggct ggtgcttcct cgacaacgtc
      541 tcgcaggacg agtacgccgg tcacctgttc gcactcggcg cggtcgcccg cctcgtcgac
      601 gacgccgagc tccatgcgat cgcggtcgat ctgctcgatc agatcggcac gcacctcgtc
      661 gcgaatcaca tgtcgtttat cgactgggac ggccggccga cccagtacgg caagctgttc
      721 cccgacggcg gcggcgacac gcccgggtac ctcgccgtgc tcggcgcgag cgcgctcgcg
      781 atcacggcgc gtggcaccgg cgatcccgcg cttgccgacg cgtacgccgc gctcgcaccg
      841 gtgaacgcga cgtacctcga tcagatcatg gtctggagcg gttctgacgg ctgcacctcg
      901 aactggaacg acatctcgat gctgaccgca gcgttccatc tcctgctgtg gaatgacagc
      961 gccgcttcgc gcgctccgtt cctcgccgcg ttcgagcgcg agctcgtgac ccagccgcgc
     1021 gggatcacgg tcgagcacaa cgcctggtac gacttgatgt gggcgatgca gaaaccgctc
     1081 ggccccggca ccgacggccc ggcatacgcc gtggtcgagg acgccgtctg tcagctccgc
     1141 gagttcccgc ggtcgaatca cgtcgtcgcg cacgacacca cggcgctcgc ggcggaggcg
     1201 tgcaccggcc ggcaaggtga gtcactcgca atgtccgcgt tcgcgaccgc ggatcgctgc
     1261 gccgcgacgt atctgtggtg gggagatcca tacgtgcgcg cgagctgcac gacggatccg
     1321 acgctcgtgc agaatcccgg cggctacctg ctgccgtact ggatggctcg ctacgccggg
     1381 ttcgtcgcgg ccgcgcagta gccaggcgtc gtgtatgtga gcacgtgctc gcgaaggact
     1441 cgttgacacg tgcgcacgaa gcagggacgc tcgcgggaca ccgttcgact gcgggaccaa
     1501 gatcatcgtc ccgggagctc ggccgcgagc gagctctaca gaagatgttc ggcggctgct
     1561 gctcgacgcg catgccgcta ggcggcccgt tcg
//
LOCUS       DASVII010000551         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_205027,
            whole genome shotgun sequence.
ACCESSION   DASVII010000551 DASVII010000000
VERSION     DASVII010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205027"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VF309_07215"
     CDS             <1..588
                     /locus_tag="VF309_07215"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7466411.1"
                     /translation="LEGLDVELVDGLLVNKHPHNPDPIHRIDVGTYERMVATGALEGL
                     PLELLEGLLVEVSPQGEEHAAAIVYLTRHLGRARAGLRVQLPLETAWGEVPEPDLALV
                     ERSHPRHRHPRTALLAVEVAVTSHKQDRETKASMYAWAPVPLYWLVDVPAKAVEVRTE
                     PGPDGYGRCEIYGVGTKVPSPADGVDDLDVTALFA"
     gene            681..>1585
                     /locus_tag="VF309_07220"
     CDS             681..>1585
                     /locus_tag="VF309_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466412.1"
                     /translation="MAAGNQIVVSGAREHNLKDISLTLPRDALVVVTGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGQMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWSRVGKPHCPICGRPIVGQSAEQIIDQVMELAEGARFMVLAPVVRG
                     RKGEYGKLLEELRADGYARVKIDDRVRLLEESIVLDKRYKHDISVVVDRLAMRHDVRK
                     RLADSIETAVGLADGLVEIEIVGSGREASAGGSVGDDAAGGGGKGAGQAKGAKGSRAA
                     GGQIVPGALPDAGTLFTFSERFACP"
BASE COUNT          256 a          493 c          584 g          252 t
ORIGIN      
        1 ctggaaggtc tcgatgtcga gctggttgat ggcctgcttg tcaacaagca cccccacaac
       61 cccgacccga ttcaccggat cgatgtgggg acctatgaac ggatggtcgc gacgggcgcg
      121 ctggagggcc tgccgctcga actgcttgaa gggctgctcg tcgaggtgag cccgcagggt
      181 gaggagcacg cggcggcgat tgtgtacctg acgcgtcatc ttgggagggc gcgtgccggg
      241 ctgcgcgttc aactcccgct ggaaaccgcg tggggcgagg tgcctgagcc cgatctggca
      301 cttgtggaga ggtctcaccc gcggcatcgg cacccccgca ccgcgttgct ggccgtcgag
      361 gtcgcagtga cctcgcacaa gcaggatcgc gagacgaagg cgagcatgta cgcgtgggcc
      421 ccagtgccct tgtactggct cgtggacgtg cccgccaagg ccgtcgaggt tcgcaccgag
      481 cccggcccgg atggctacgg ccgctgcgag atctacggtg tcggcacgaa ggtgccgtca
      541 ccggccgacg gcgtggacga tctcgacgtg accgcgctgt tcgcctgatc gacgcggacg
      601 agcgcgaatc gcgcaaattg cgctcgcagt cagccgtgcg ctaggcgaac gtctgttcgc
      661 cgtcatagaa tgaacggcag atggcagcgg gcaatcagat cgtcgtctca ggggcgcgtg
      721 agcacaacct gaaggacatt tcgctgacgc tgccgcgcga cgcgctggtg gtcgtcacgg
      781 ggctgtcggg gtcgggtaag tcgagcctcg ctttcgacac gatctacgcg gaggggcagc
      841 gccgttatgt ggagtcgctg tcggcgtacg cgcgccagtt cctcgggcag atggacaagc
      901 cggacgtgga ctcgatcgag gggctctcgc cggcgatctc gatcgaccaa aagaccactt
      961 cacgcaaccc gcgctcgacg gtcggcacgg tcaccgagat ctacgactac ctgcgcctct
     1021 tgtggtcgcg cgtgggcaag ccgcactgcc cgatctgcgg gcgcccgatc gtgggacagt
     1081 cggcggagca gatcatcgac caggtgatgg agctcgcgga gggcgcgcgc ttcatggtgc
     1141 tcgcgcctgt ggtgcgaggg cgcaagggcg agtacgggaa gctcttggag gagctgcgcg
     1201 cggacggcta cgcgcgcgtg aagatcgacg accgcgtgcg tctgctcgag gaatcgatcg
     1261 tgctcgacaa gcgctacaag cacgacatct ccgtcgtcgt ggatcgcctc gcgatgcgcc
     1321 acgacgtgcg caagcgcctc gcggactcga tcgagacggc ggtcggcctg gcggacgggc
     1381 tcgttgagat cgagatagtg ggctccggcc gggaggcctc cgccggcggg tcggtcggcg
     1441 acgacgccgc aggaggcggt ggcaagggcg cggggcaggc gaaaggcgcg aagggctcac
     1501 gcgccgcggg tgggcagatc gtgcccggcg cgctgccgga tgccggcacg ctgttcacgt
     1561 tctccgagcg cttcgcgtgc cccga
//
LOCUS       DASVII010000552         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_26227, whole
            genome shotgun sequence.
ACCESSION   DASVII010000552 DASVII010000000
VERSION     DASVII010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26227"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VF309_07225"
     CDS             <1..1638
                     /locus_tag="VF309_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7466413.1"
                     /translation="TQGTWYAHASVGCLHVRPVLNMKGDGALRMRAIAEEAADLVRRY
                     KGSYSGEHGDGLVRSEWIAPFFGPRLTASLGEIKSWFDPKGIMNPGKIVSPSRQDDRS
                     LFRYGPNYKQPSPRTALDWNAWGGFAGAVEMCNNNGHCRKFDAGTMCPSFRATGDERD
                     LTRGRANTLRLALSGQLGADGVAEEAVRDALDLCVSCKGCRRECPTGVDMARMKIEFL
                     SHYKRRHGFTLRDKLIGHLPRYAKWAARVQPLASLATRLPGAGAVMESLGFDRRRAFP
                     VFKAPWTKKRGARGEAPEANEAGRGTRRRIVLFADTFNNWFEPGNLDAALKVLEATGH
                     EVVTPAGDGGRALCCGRTYLAAGMIDEARAEAKRTMAALKPHLEAGAPVVGLEPSCLF
                     TFRDEYAAMFPGDALAGLLAKAALVDEYLLAEIRGGRVVPAWKSAGAKEIRVHGHCHQ
                     KAFDTFEATLELLRTIPDARVSAIESSCCGMAGAFGHEKGHYDVSMKMAELALLPAVR
                     AAPAATIVAAGTSCRRQIVDGSGREALHPLALLAQML"
     gene            1635..>1944
                     /locus_tag="VF309_07230"
     CDS             1635..>1944
                     /locus_tag="VF309_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466414.1"
                     /translation="MSAIPGAPILGALRAALAFEPTGAASGNVALGRGNFEGRPLHVA
                     LVENRGASGALGALECQRLAALFKIVAAERSSLVLYLDSAGAKGSEGLKALGAFRSLY
                     R"
BASE COUNT          288 a          637 c          728 g          291 t
ORIGIN      
        1 acccagggca cctggtatgc acacgcgtcg gtgggatgcc tgcacgtgcg cccggttctc
       61 aacatgaagg gcgatggcgc gctgcggatg cgtgccatcg ccgaggaggc ggccgacctg
      121 gtgcgccgct acaagggttc ctattccggc gagcacggcg acggcctcgt gcgctccgaa
      181 tggatcgcgc cattcttcgg gccgcgcctc accgcctcgc tcggcgagat caagtcgtgg
      241 ttcgatccga agggaatcat gaatcccggc aagatcgtga gcccgtcgcg gcaggacgat
      301 cgctcgctgt ttcgctacgg gccgaactac aagcagcctt cgccgcgcac ggcgctcgac
      361 tggaatgcgt ggggcggctt cgcgggggcg gtcgagatgt gcaacaacaa cgggcactgc
      421 cgcaagttcg acgcgggaac catgtgtccc agctttcgcg cgacgggcga cgagcgcgat
      481 ctcacgcgcg ggcgggccaa cacgctgcgg ctggcgctct cggggcagct gggcgcggac
      541 ggcgtcgccg aggaagcggt gcgcgatgcg ctcgatctct gcgtgtcgtg caagggatgc
      601 cggcgcgagt gcccgaccgg cgtggacatg gcgcgcatga agatcgagtt cctctcgcac
      661 tacaagcggc gccacggatt cacgctgcgc gacaagctga tcgggcacct gccgcgctat
      721 gcgaaatggg cggcgcgggt ccagccgctg gcgagcctcg cgacgcgctt gcccggcgcg
      781 ggcgcggtca tggagtcgct cggcttcgat cggcggcggg cgtttccggt cttcaaggcg
      841 ccgtggacga agaagcgcgg cgcgagaggc gaggcaccgg aggccaacga agcggggcgc
      901 ggcacgcggc gccggatcgt cttgttcgcg gacaccttca acaactggtt cgagcccggc
      961 aacctcgatg cggcgttgaa agtgctggag gcgacgggac acgaagtcgt gacgccggcg
     1021 ggcgacggcg ggcgggcgct ttgctgcggg cgcacgtacc tcgcggcggg catgatcgat
     1081 gaagcgcgcg ccgaggcgaa gcgcaccatg gcggcgttga agccgcacct cgaagcgggc
     1141 gcgccggtcg tgggcctgga gccctcgtgc ctcttcacgt tccgcgatga atacgcggcg
     1201 atgtttcccg gcgatgcgct cgcggggctg ctcgcgaagg ccgcgctcgt cgacgaatat
     1261 ctcctcgcgg agatccgcgg gggacgcgtc gtgcccgcct ggaagagcgc gggcgcgaag
     1321 gagattcgcg tgcacggcca ttgccaccag aaggcgttcg acacgttcga ggcgacgctc
     1381 gaattgcttc gcacgattcc ggacgcgcgg gtgagcgcga tcgagtcgag ctgctgcggc
     1441 atggcgggcg ccttcgggca cgagaagggc cactacgacg tgtcgatgaa gatggccgag
     1501 ctcgcgctcc tgcccgcggt gcgcgccgcg ccggcggcca cgatcgtcgc ggcgggcacg
     1561 agctgccggc ggcagatcgt cgacggctcg ggacgcgagg cgctccatcc cctcgcgctt
     1621 ctcgcgcaga tgctgtgagc gcgattccgg gcgcgccgat cctgggagcg ctgcgcgcgg
     1681 cgctcgcgtt cgagccgacg ggcgctgcct cgggaaacgt cgccctcggc cgcggaaatt
     1741 tcgaagggcg gccgctgcac gtcgcgctgg tcgagaatcg cggcgcgagc ggcgccctcg
     1801 gcgcgctcga gtgccagcgc ctcgcggcgc tcttcaagat cgttgccgcg gagcgctcga
     1861 gcctcgtcct gtatctcgac agcgcgggag ccaagggttc cgaagggctc aaggcgctcg
     1921 gcgctttccg atcgctctat cgcg
//
LOCUS       DASVII010000553         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_492921,
            whole genome shotgun sequence.
ACCESSION   DASVII010000553 DASVII010000000
VERSION     DASVII010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492921"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF309_07235"
     CDS             complement(<1..203)
                     /locus_tag="VF309_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466415.1"
                     /translation="MSEIPPSYAVRRRPWLWPAIIVAAVAVVALGAWLIWRPSSDDAA
                     KAQGSDAAKAGKGAGKSGKGAGKA"
     gene            complement(329..1330)
                     /locus_tag="VF309_07240"
     CDS             complement(329..1330)
                     /locus_tag="VF309_07240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX7466416.1"
                     /translation="MNDPHPYSRLTPDTVLNSLESVGLLCDGRLLALNSYENRVYQVG
                     LDDGSLVVAKFYRPARWSDAQILEEHGFALELAQAEIPVVAPLELAGATLHHASGFAF
                     AAFPRRGGRSPELDDGETLKWLGRFIGRIHALGAVRPYAHRPALDIESFGDEPRAFLL
                     GSGLVPADLLPAWKQASELALEGVRHCFQRAGKVAMIRTHGDCHASNVLWTPDSGNSR
                     GGPHFVDLDDSRMAPAVQDLWMLISGDRHAMGQQLGKLLEGYEDFSEFDDRELHLVEA
                     LRTLRLLHYSAWIARRWDDPAFPIAFPWFNTARYWQDRVLELREQIAAMQEPALVPS"
     gene            1534..2043
                     /locus_tag="VF309_07245"
     CDS             1534..2043
                     /locus_tag="VF309_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX7466417.1"
                     /translation="MSWEFIQHGLARWVHIMAGVMWIGLLYYFNFVQVDALKKAAADT
                     PPTGAGITKHVAPRALLFFRWAAVVTVVLGLFILGDKADDALFFKERAYVPIGIGGWL
                     GLIMLFNVWVLIWPNQKKILGLVAATDEEKNKARRVAFLASRTNTALSIPMLFFMSSS
                     YLFHAAMFA"
     gene            complement(2086..>2405)
                     /locus_tag="VF309_07250"
     CDS             complement(2086..>2405)
                     /locus_tag="VF309_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7466418.1"
                     /translation="IAASDPQRMQGLMYRKKLGRDDGMLFVFDEPAYQSMWMKNTLIP
                     LSVAFIDADGRILNILDMEPQTLETHTSAGPAVYAIETNKGWFDENRVKPGEKVTGLP
                     KPK"
BASE COUNT          386 a          777 c          818 g          424 t
ORIGIN      
        1 gccttgcccg cgcccttgcc ggacttgccc gcgcccttgc cggccttggc ggcgtcgctg
       61 ccctgggcct tggcggcgtc gtcgctcgag gggcgccaga tgagccacgc gccgagggcg
      121 acgacggcga cggccgccac gatgatggcc ggccacagcc acgggcggcg gcgaacggcg
      181 taagacgggg gaatctcgga catggctctc gggtcttttt cgaatcagcg attggagcgg
      241 atattctgtt gccagttcgt gccgaggtgc taagaaatgt aagggcacgt cccggcggcg
      301 cgcttcggga cggcagcggc gccccgccct acgaggggac cagcgcgggc tcctgcatcg
      361 cggcgatctg ctcgcgcagc tccagcaccc gatcctgcca gtagcgcgca gtattgaacc
      421 atggaaacgc gatcgggaat gcggggtcgt cccagcgccg cgcgatccac gcggagtagt
      481 gcagcaggcg cagggtgcgc agggcttcca cgagatgcag ctcgcgatcg tcgaactcgc
      541 tgaagtcctc gtacccctcg aggagcttgc cgagctgctg gcccatggcg tggcgatcgc
      601 ccgagatgag catccacagg tcctgcaccg ccggcgccat gcggctgtcg tcgaggtcga
      661 cgaaatgcgg gccgccccgc gagtttccgc tgtccggcgt ccacagcacg ttgctcgcgt
      721 ggcagtcgcc atgggtgcgg atcatcgcga ccttgccggc gcgctgaaag cagtggcgca
      781 caccttcgag cgcgagctcc gaagcctgct tccacgcggg aagcagatcg gcgggaacga
      841 gcccgctccc gagcaggaac gcgcgcggct cgtcgccgaa gctttcgatg tcgagcgcgg
      901 gccgatgcgc atagggccgg acggctccga gcgcatggat gcgcccgatg aaacgcccga
      961 gccacttgag cgtctcgccg tcgtcgagct ccggcgagcg cccgccgcgg cgcggaaatg
     1021 cggcgaaggc gaagccggag gcgtgatgca gcgtcgctcc ggcgagctcg agaggcgcga
     1081 ccacgggaat ctcggcctgg gcgagctcga gggcgaagcc atgctcctcg agaatctgcg
     1141 cgtcgctcca gcgcgcgggg cgatagaact tggcgacgac cagggacccg tcgtcgagac
     1201 ccacctgata cacgcggttc tcgtagctgt tcagcgcgag caggcgccca tcgcaaagga
     1261 gccccacgct ttcgagcgag ttgaggaccg tgtcgggagt caggcgcgag tacggatggg
     1321 gatcattcac cgatccattc taaccccttc aatcgttagg gcttttcgac gcccgcgaac
     1381 agcgtgcgtg ctagaatcgc ctcacgtgcc gtctcacttt caggagcatg actatgaaaa
     1441 atcccttgga tacgctttgg ggcaccgtga tcacgggcct cgtcctgacg gtggttctct
     1501 actacctcgc tcgcgcgatg atgggaggct gacatgtcct gggaattcat ccaacacggt
     1561 ctcgcgcgct gggtccacat catggcgggc gtcatgtgga tcggccttct ctactacttc
     1621 aacttcgtcc aggtcgacgc gctgaagaag gccgcggccg acacgccgcc gacgggcgcg
     1681 ggcatcacca agcacgtcgc gccgcgcgct ctgctcttct tccgctgggc ggcggtggtg
     1741 acggtggtgc tgggactgtt catcctcggc gacaaggccg acgacgccct cttcttcaag
     1801 gagcgcgcct acgtgccgat cggcatcggc ggatggctcg ggctgatcat gctcttcaac
     1861 gtgtgggtgc tcatctggcc caaccagaag aagatcctcg ggctcgtggc cgcgaccgat
     1921 gaggagaaga acaaggcgcg ccgcgtcgcg ttcctcgcgt cgcggaccaa cacggcgctt
     1981 tccatcccga tgctcttctt catgtcctcg tcgtatctct tccacgcggc gatgttcgcg
     2041 taggcgaggc gcggcgggga aaaaagggcg ccgcggcgcc ctttttcatt tgggcttcgg
     2101 cagccccgtc accttctcgc cgggcttcac ccggttctcg tcgaaccagc ccttgttggt
     2161 ctcgatcgcg tagacggcgg ggcccgcgga ggtgtgcgtc tcgagggtct ggggctccat
     2221 gtcgaggatg ttgaggatgc gcccgtcggc gtcgatgaac gcgaccgaga gggggatcaa
     2281 cgtgttcttc atccacatgc tctggtatgc gggctcgtcg aagacgaaga gcatcccgtc
     2341 gtcgcggccg agcttcttgc ggtacatgag gccctgcatg cgctggggat cggaggccgc
     2401 gattt
//
LOCUS       DASVII010000554         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_205117,
            whole genome shotgun sequence.
ACCESSION   DASVII010000554 DASVII010000000
VERSION     DASVII010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205117"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VF309_07255"
     CDS             complement(<1..1212)
                     /locus_tag="VF309_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX7466419.1"
                     /translation="MSPVLRTEIFKAYDIRGLYSEQIDGDVAELIGRAFARVLAQLAG
                     KPVGELRIGLGRDMRLSAPELAARYRAGMLSQGVDVVDAGQVGSEMLYFLVGSRGLDG
                     GLMCTASHNPKAYTGAKLVRAGAIALSGDSGIQDIRREVEAELSGGAAGSGPGEPSGS
                     VEEVDLYAEFQEAALRFVDPDVIRAGNDGTPLKVVLDGGNGMAGPMVGPLLDRLRLDL
                     LEIYWTPDGNFPDHEPNPLLPENRELIIAKVRASGADLGIAWDGDGDRCFFIDDTGRF
                     VDGDFLTALLADSLLRKNPGEAILYDVRASRAVPDTVTAAGGTPYRNRVGHAFFKTRM
                     RDEGSLFGGEVSGHYYFRDFYCADSGTIPALLVLELLCGEGKSLSQLLAPYHSRYFIS
                     GEVNSEVVDPQA"
     gene            1297..>1969
                     /locus_tag="VF309_07260"
     CDS             1297..>1969
                     /locus_tag="VF309_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7466420.1"
                     /translation="MSGHSKWASIKHKKAIVDSRRGAAFSKLTRAITVAARDGGGDPV
                     GNPSLDLAIRKAKEASMPKDNIERAIAKGTGDGGEADAIESVLYEGYGPGGVALLVEA
                     LTDNRNRTSADVRHAFSKSGGNLGEPGSVAYQFDKKGTIVIDGTRYSEDELMLAVDAG
                     AEDIVTDEEVFEVITEPADFAAVRKALEDAGVEMQSAELAYRPSALVPVDEAQAGKLM
                     RLIDAL"
BASE COUNT          319 a          703 c          668 g          279 t
ORIGIN      
        1 cgcttgcgga tccacgacct ccgagttgac ctcgcctgag atgaagtagc gcgagtggta
       61 gggggcgagc agttgcgaca gcgacttgcc ctcgccgcac agcagctcga gcacgagcag
      121 cgccgggatc gtgcccgagt ccgcgcagta gaagtcgcgg aagtagtagt gccccgagac
      181 ctcgccgccg aacagcgagc cctcgtcgcg catccgcgtc ttgaagaacg cgtgccccac
      241 acggttgcga tacggagtcc cgccggcggc ggtgacggtg tccggcaccg cgcgcgaagc
      301 gcgcacgtcg tacaggatcg cttcgccggg gtttttacgc agcagcgagt ccgcgagcag
      361 cgcggtgagg aagtccccgt ccacgaacct gcccgtgtcg tcgatgaaga agcagcggtc
      421 cccatcccca tcccacgcga tgccgaggtc cgcgccgctc gcgcgcacct tcgcgatgat
      481 gagctcacgg ttctccggca gcagcgggtt cggctcgtga tcggggaagt tgccgtcggg
      541 agtccagtag atctcgagga gatccaggcg gaggcggtcg agcagcgggc cgaccatcgg
      601 gccggccatg ccgttaccgc cgtcgagcac caccttcagc ggcgtgccgt cgttgcccgc
      661 gcggatcacg tcgggatcga cgaaccgcag cgcggcctcc tggaactccg cgtacaggtc
      721 gacctcctcg acgctgccac tcggctcgcc gggaccgctc ccggccgctc cgccgctcag
      781 ctcggcttcg acctcgcggc ggatgtcctg gatgccggag tcgcccgaca gcgcgatcgc
      841 gcccgcgcgc acgagcttcg cgccggtgta cgccttgggg ttgtgcgagg cggtgcacat
      901 cagcccgccg tccagcccgc gcgagccgac gaggaagtac agcatctcgc tgcccacctg
      961 gcccgcgtcc accacgtcaa cgccttgcga gagcatgcct gctcggtagc gcgccgcgag
     1021 ctccggcgcg ctcaagcgca tgtcgcgccc cagcccgatg cgcagctcgc ccaccggctt
     1081 gcccgcgagc tgggcgagca cgcgcgcgaa cgcgcggccg atcagctcgg cgacgtcgcc
     1141 gtcgatctgc tcgctgtaga ggccgcggat gtcgtacgcc ttgaagatct ctgttctcag
     1201 gactgggctc atgcgcccga gcatcctaac cggcccgatc gcgctttccg ggtgcgccga
     1261 acgcctccgt gcgcggctgt tcgtagactt ccgtacatgt ccgggcattc caagtgggcg
     1321 agcatcaagc acaagaaggc gatcgtggac tcgcgccgcg gcgccgcgtt ctcgaagctc
     1381 acgcgcgcga tcaccgtcgc ggcacgcgac ggcggcggcg atcccgtggg caacccgtcc
     1441 ctggatctcg cgatccgcaa ggccaaggaa gcgtcgatgc cgaaggacaa catcgagcgc
     1501 gcgatcgcca agggcaccgg cgacggcggc gaggcggacg cgatcgaaag cgtcctctac
     1561 gagggctacg gccccggcgg agtggctctg ctggtggagg cgctgaccga caaccgcaac
     1621 cgcacgagcg ccgacgtgcg ccacgccttc tccaagagcg gcggcaacct cggcgagccc
     1681 ggctccgtgg cctaccagtt cgacaagaag ggcacgatcg tgatcgacgg cacgcgctac
     1741 tccgaagacg agctcatgct cgccgtcgac gccggcgcgg aggacatcgt caccgacgag
     1801 gaggtcttcg aggtcatcac cgagccggcc gacttcgcgg ccgtgcgcaa ggccctcgag
     1861 gacgccggcg tggagatgca gagcgccgag ctggcctacc ggccctccgc gctcgtgccc
     1921 gtggacgagg cacaggcggg caaactcatg cgcttgatcg acgcgctcg
//
LOCUS       DASVII010000555         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_636740,
            whole genome shotgun sequence.
ACCESSION   DASVII010000555 DASVII010000000
VERSION     DASVII010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636740"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF309_07265"
     CDS             <1..316
                     /locus_tag="VF309_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466421.1"
                     /translation="DIAIRSSQRDIGQLVLDVIDGKLTAAAIAIPFSHAVAAAREAAW
                     AQGRVLTFPVPLRFHAVAGVSRDGVPAGLITLGPSPEGLDKVLAELHDASANRKMLAQ
                     QR"
     gene            complement(350..1135)
                     /locus_tag="VF309_07270"
     CDS             complement(350..1135)
                     /locus_tag="VF309_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7466422.1"
                     /translation="MAALRIERASGVLRIDMARPEVLNAFDEAMIAELDEAFAIHGSD
                     PDVRVIVLGGQGKAFSAGADLGWMKRQSEASAEANLEDARRFAGMLRRISECPRPTVA
                     RVQGVAMGGGVGLVCACDFAIAGEEARFAVTEARFGLAASVIGPYLIAAVGRRQARRL
                     AMSTAQIGAAEALAIGLVHEVAPPERLDHALEGLATQLRASGPRAIAEIKALYGRLPP
                     APVTPETLELTAQTIARIRHTEEAREGFTAFLEKRRPKWIDDG"
     gene            complement(1135..>2086)
                     /gene="boxB"
                     /locus_tag="VF309_07275"
     CDS             complement(1135..>2086)
                     /gene="boxB"
                     /locus_tag="VF309_07275"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX7466423.1"
                     /translation="VYLLHRYFGRDGREEAEALLERRSGDADHPRILGAFNERTPDWL
                     AFFMFTHFTDRDGKFQLKALAESGFDPLARTTKFMLTEEAHHMFVGEAGVSRVLQRTC
                     EVMNELRTDDPARLRAAGVIDLPTVQRYPNFHYSVTLDLFGADESSNAATFYATGIKG
                     RFDEAKHDDDHRLRGDSYEILDVKDGRLAPRRVPMLNALNEVLRDDFVRDSVGGIERW
                     NKRIEKTGVAFRLALPHKAFHRRIGTLANVSVSPEGEVLTRERWAANADRWLPTEGDR
                     AFVASLMGRVVEPGRFAGWIAPPAIGIDRHPIDFEYVRFG"
BASE COUNT          307 a          734 c          727 g          318 t
ORIGIN      
        1 cgacatcgcg atcaggtcct cgcagcgcga catcggacag ctcgtgctcg acgtgatcga
       61 cggcaagcta accgctgcgg cgatcgccat cccgttttcg catgcggtcg ccgcggcgcg
      121 cgaggcagcg tgggcgcaag gtcgcgtgct caccttcccc gttcccctac ggttccacgc
      181 ggtcgcggga gtgtcgcgcg acggcgtgcc ggcgggattg atcacgctgg gtccatcgcc
      241 ggagggcctg gacaaggtgc tggccgagct gcacgacgca tcggcgaacc ggaagatgct
      301 cgcccagcaa cggtagacct ggcgcacgcg cctcgcgcgg gcgagggcct cagccgtcat
      361 cgatccactt cggccggcgc ttctcgagaa aggccgtgaa gccctcgcgc gcctcctcgg
      421 tgtggcggat gcgcgcgatg gtctgcgccg tgagctcgag cgtctccggc gtcacgggcg
      481 ccgggggcag ccgcccgtag agcgccttga tctcggcgat ggcgcgcgga ccgctcgcgc
      541 gaagctgcgt cgcgagacct tccaacgcgt gatcgagccg ctccggcggc gcgacttcat
      601 gcacgaggcc gatcgcgagc gcctccgccg cgccgatctg ggcggtgctc atggcgagcc
      661 gccgcgcctg tcttcgcccg accgcggcga tgaggtaggg cccgatcacc gaagcggcga
      721 ggccgaagcg cgcttcggtc acggcgaagc gcgcttcctc gcccgctatc gcgaagtcgc
      781 atgcgcacac caggcccacg cctccgccca tcgcgacgcc ctgcacgcgc gcgaccgtgg
      841 gcctggggca ctcggagatg cgccgcagca tccccgcgaa gcggcgcgca tcctcgagat
      901 tggcttcggc gctcgcctcg ctctggcgct tcatccatcc gaggtcggcg cccgcggaga
      961 aggccttccc ctgccccccg aggacgatga cgcgcacatc cggatcggag ccatggatcg
     1021 cgaaggcctc gtcgagctcg gcgatcatcg cttcgtcgaa tgcgttgagc acctcgggac
     1081 gcgccatgtc gatgcgcaac acgccggagg cgcgttcgat gcgcagggcc gccatcagcc
     1141 gaagcgcacg tactcgaagt cgatcgggtg gcgatcgatg ccgatggcgg gcggcgcgat
     1201 ccatcccgcg aagcggcccg gctcgaccac tcgccccatg agggaggcga cgaacgcgcg
     1261 atcgccctcg gtgggcagcc atcggtcggc gttcgccgcc caccgctcgc gcgtgaggac
     1321 ctcgccctcg ggagagacgc tcacgttggc gagcgtgccg atgcggcgat ggaatgcctt
     1381 gtggggcaac gcgaggcgga aggcgacgcc cgtcttctcg atcctcttgt tccagcgctc
     1441 gatgccgccc acggagtcgc ggacgaagtc gtcgcgcagc acctcgttca gcgcgttcag
     1501 catcggcacg cggcgtggcg ccaggcgccc gtccttcacg tcgaggatct cgtacgagtc
     1561 gccgcgcagc cgatggtcgt cgtcgtgctt cgcctcgtcg aagcggccct tgatgcccgt
     1621 cgcgtagaag gtcgcggcat tgctcgactc gtcggcgccg aagaggtcca gcgtcacgct
     1681 gtagtggaag ttgggatagc gctgcaccgt cggcaagtcg atcacccccg cggcgcgcag
     1741 ccgcgcggga tcgtcggtgc gcagctcgtt catcacttcg caggtgcgct ggagcacgcg
     1801 cgacacgccc gcctcgccga cgaacatgtg atgcgcctcc tcggtgagca tgaacttcgt
     1861 cgtgcgcgcg agcgggtcga agccggactc ggcgagcgcc ttcagctgga acttgccgtc
     1921 gcggtcggtg aagtgcgtga acatgaagaa cgcgagccag tcgggcgtgc gctcgttgaa
     1981 ggcgccgagg atgcgcggat ggtccgcatc gccggagcgc ctttccaaca atgcctcggc
     2041 ctcctcgcgg ccgtcgcggc cgaaatagcg gtgcagcagg tacacc
//
LOCUS       DASVII010000556         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_457078,
            whole genome shotgun sequence.
ACCESSION   DASVII010000556 DASVII010000000
VERSION     DASVII010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457078"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VF309_07280"
     CDS             complement(<1..904)
                     /locus_tag="VF309_07280"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7466424.1"
                     /translation="MPHIPASKALARFRVVDMTQVRAGPTAARQLADWGADVIQVQMP
                     EHMRGDDTLGGQEGSDYQYTHRNKRSITLNLKEPAGIDALKKLVATADVLLENFRPDV
                     KTRLGIDYDALSKVNPRLVYASISGFGQTGPYATRPGFDQIAQGMGGLMSVTGLPGQG
                     PLRVGLPIADLCAGIFAAYGVTVALLERDVSGKGQWLHTSLLEAQIYMMDFQTSRWTL
                     DHEIPGQAGNFHPTSIPTGVYRTKDGYINIAVFGGKIWERFAEAMGAPEWITDERSKT
                     KAGRSVHRDWLNAEIEKRLASNGSS"
     gene            complement(931..>1676)
                     /locus_tag="VF309_07285"
     CDS             complement(931..>1676)
                     /locus_tag="VF309_07285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7466425.1"
                     /translation="ALSPNFAWAQLVKPDDNRITTGYASYLAPKGSGNMRGYLALPAA
                     AKGRRATVVVIHENRGLNPYIEDVARRLAVEGYTAFAPDALTPVGGYPGDEDKARDMF
                     AKQDGAKRTEDLLAAADYVKLRPDFSGKFGAVGFCFGGGIVNTFATRMPDLAAAVPYY
                     GAQPPAAEAAKIKSPMLIHYASDDERIDAGWPAFESVLKAHHVKYQAYIYPKTQHGFH
                     NDTTPRYDEAAARLSWQRTLAFFKEHLKA"
BASE COUNT          241 a          553 c          576 g          306 t
ORIGIN      
        1 agctgctgcc attcgacgca agccgcttct cgatctcggc gttcaaccag tcgcggtgca
       61 cggagcggcc ggccttggtc ttcgagcgct cgtcggtgat ccattcgggg gcacccatcg
      121 cctcggcgaa gcgctcccag atcttgccgc cgaagaccgc gatgttgatg tagccgtcct
      181 tcgtcctgta gacgccggtg gggatgctcg tcggatggaa gttgcccgcc tgccccggga
      241 tctcgtggtc cagcgtccag cgcgacgtct ggaaatccat catgtagatc tgcgcttcga
      301 ggagcgacgt gtggagccat tggcccttgc ccgacacgtc tctttcgaga agggccacgg
      361 tcaccccgta cgcggcgaaa attcccgcgc aaagatccgc gatgggaaga cccacccgca
      421 gcggcccctg gcccgggagc ccggtgacgg acatgagccc gcccatgccc tgcgcgatct
      481 ggtcgaagcc gggacgcgtg gcatagggac ccgtctgccc gaacccggag atgctcgcgt
      541 acacgaggcg cggattcact ttcgagagcg cgtcgtagtc gatgcccagg cgcgtcttca
      601 cgtcggggcg gaaattctcg aggagcacgt cggcggtggc gacgagcttc ttcaacgcgt
      661 cgatccccgc gggctccttg aggttgagcg tgatggagcg cttgttgcgg tgcgtgtact
      721 ggtagtccga gccttcctgc ccgcccagcg tgtcgtcgcc cctcatgtgc tcgggcatct
      781 gcacctggat cacgtcggcg ccccagtcgg cgagctggcg cgcggccgtc gggcccgcgc
      841 gcacctgggt catgtccacg acgcggaaac gcgccaacgc cttcgatgcg gggatgtgcg
      901 gcatgcgggt agtttacggg gacgcgtgcc tcaggccttc aggtgttcct tgaagaaggc
      961 gagcgtgcgc tgccacgaca gcctcgccgc cgcctcgtcg tagcgcggcg tggtgtcgtt
     1021 gtggaagccg tgctgcgtct tggggtagat gtaggcctgg tacttcacgt ggtgcgcctt
     1081 gagcaccgac tcgaacgcgg gccacccggc gtcgatgcgc tcgtcgtcgg atgcatagtg
     1141 gatgagcatc ggcgacttga tctttgccgc ttcggcagcg ggcggctgcg cgccatagta
     1201 gggaaccgcc gccgcgaggt ccggcatgcg cgtcgcgaac gtgttgacga tgccaccgcc
     1261 gaagcagaaa cccacggcgc cgaacttgcc gctgaagtcg gggcgcagtt tcacgtagtc
     1321 cgccgcggcc aagaggtcct cggttcgctt ggcgccgtcc tgcttcgcga acatgtcgcg
     1381 cgccttgtcc tcgtcgccgg gatagccgcc caccggcgtg agcgcgtcgg gcgcgaaggc
     1441 ggtgtatccc tcgacggcga gccgccgcgc cacgtcctcg atgtacgggt tgaggccgcg
     1501 gttctcgtgg atcacgacca ccgtcgcgcg ccgccccttc gccgccgcgg gaagcgcgag
     1561 gtagccgcgc atgtttcccg atcccttcgg cgcgagatac gacgcgtaac ccgtcgtgat
     1621 gcggttgtcg tcgggcttca ccagctgcgc ccaggcgaag ttggggctga gggcct
//
LOCUS       DASVII010000557         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_457093,
            whole genome shotgun sequence.
ACCESSION   DASVII010000557 DASVII010000000
VERSION     DASVII010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457093"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF309_07290"
     CDS             complement(<1..309)
                     /locus_tag="VF309_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466426.1"
                     /translation="MIGTDLDVRGGISAMVKVYVAQGLFARWDLRYIATHRDGSRVQK
                     AFKAARAWLVFAAQLLTFRIALVHVHIASDASFWRKSLFIVPAHCLGVPYVLHMHGGQ
                     F"
     gene            complement(448..>1609)
                     /locus_tag="VF309_07295"
     CDS             complement(448..>1609)
                     /locus_tag="VF309_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX7466427.1"
                     /translation="LLQNAPDGVNREQAVSYQQFELDLLLLPLLAARANGADFPPAYV
                     ARIEAMLVYLASVIDSGGNVPMFGDADDGFAVRLDPRPGFSRYHSLLATGAILFGRGD
                     FKAKAGALDDKTLWLFGAGARDAFAKIDTANARLPVRREFPDGGYYILGCDFETGDEI
                     RLVADAGALGYREIAAHGHADALSFTLSMAGEEFLIDPGTFAYHTEAEWRAYFRGTSA
                     HNTVRVDGRDQSQQGGNFMWLRKARAGCSQWITSAGEDVFEGWHDGYTALADPVMHRR
                     RIVLDKTTRRIVIEDTLQMEGEHEVEIFLHAHEKCAVSMGTGGTRLARGDRALDIRWP
                     KVRAATTQMLHGSESPIGGWVSRAFDRKEPAPTLVWRARLSGNQRLRTEIDC"
BASE COUNT          264 a          542 c          543 g          260 t
ORIGIN      
        1 gaactggccg ccgtgcatgt gcagcacgta aggcacaccc agacagtgcg caggcacgat
       61 gaagagggac ttgcgccaga agctcgcgtc gctcgcgatg tgcacgtgca cgagcgcgat
      121 gcggaaagtg aggagctgcg cggcgaacac aagccatgcg cgcgccgcct tgaaggcctt
      181 ctgaaccctg gatccgtcgc ggtgcgtcgc gatgtatcgc aagtcccaac gcgcgaaaag
      241 accctgagcc acatacactt tgaccattgc cgagatgccg ccgcgcacat cgaggtcggt
      301 accgatcatc aggatctttt ttctcgacat cttccccgcc atgccaccgc gttcattgat
      361 gaaatcaatc atttttttcg ttgatttcgt caatgatctc cccagactcg tccctggtcg
      421 agcggatcga ctgagtcgac tgtcgcgtca gcagtcgatc tcggtgcgca gccgctggtt
      481 gccggagagc cgcgcgcgcc acaccaaggt cggcgcgggc tccttgcggt cgaaggcgcg
      541 cgacacccag ccgccgatcg gggactcgct gccgtggagc atctgcgtcg tcgcggcccg
      601 caccttcggc cagcggatgt cgagggcgcg gtcgccgcgc gcgaggcgcg tgccgccggt
      661 gcccatgctc accgcgcatt tctcgtgggc gtgcaggaag atctcgacct cgtgctcgcc
      721 ttccatctgc agcgtgtcct cgatcacgat gcggcgcgtg gtcttgtcga gcacgatgcg
      781 ccggcgatgc atcaccggat cggcgagcgc cgtgtagccg tcgtgccagc cctcgaagac
      841 gtcctcgccc gccgaggtga tccattgcga gcagccggcg cgcgccttgc gcagccacat
      901 gaagttgccg ccctgctgcg actggtcgcg cccgtccacg cgaaccgtgt tgtgcgccga
      961 cgtgccgcgg aagtacgcgc gccactcggc ctcggtgtga taggcgaacg tgcccggatc
     1021 gatgaggaac tcctcgcccg ccatcgacag cgtgaaggaa agcgcgtcgg cgtggccgtg
     1081 ggcggcgatc tcgcgatagc cgagcgcccc cgcgtcggcc accaggcgga tctcgtcgcc
     1141 ggtctcgaaa tcgcagccga ggatgtagta gccgccgtcg ggaaactcgc ggcgcaccgg
     1201 caggcgcgcg ttcgcggtgt cgatcttcgc gaacgcgtcc cgggcgcccg ccccgaaaag
     1261 ccagagcgtc ttgtcgtcga gcgccccggc cttcgccttg aagtcgccac gcccgaagag
     1321 gatggcgccc gtggcgagga gcgagtggta gcggctgaat ccggggcgcg ggtcgagacg
     1381 caccgcgaag ccgtcgtccg cgtcgccgaa catcggcacg ttgccgccgg agtcgatcac
     1441 cgacgcgagg tagaccagca tcgcctcgat gcgcgccacg tacgcgggcg ggaagtcggc
     1501 gccgttggcg cgcgcggcaa gaagcggcag gagcagcagg tcgagctcga actgctggta
     1561 ggagacggcc tgctcgcgat tgaccccgtc gggcgcgttc tgcagcagc
//
LOCUS       DASVII010000558         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_205271,
            whole genome shotgun sequence.
ACCESSION   DASVII010000558 DASVII010000000
VERSION     DASVII010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205271"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF309_07300"
     CDS             <1..523
                     /locus_tag="VF309_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7466428.1"
                     /translation="LDEAGVAYRVNARLVRGLDYYNRTVFEWISPEIGAQSTLAGGGR
                     YDALFEQLGGKPTPACGFGMGIERVLLAWQAADGHAAYEPDVFVVHAGEAAEIEAWKA
                     SEKLRDAGLSVILGAGGSFKSQMKKADASGARYAVILGEDEVSAAKLTVKSLRGAGEQ
                     KMLALREAIEHIR"
     gene            533..1171
                     /locus_tag="VF309_07305"
     CDS             533..1171
                     /locus_tag="VF309_07305"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7466429.1"
                     /translation="MSGSYDFEEQERLAELKAWWEDNRWYVVGAVVAAAVAFSGYRGW
                     IYWKASTAEDAAAMYQPVEEALKANDAKKVPETAQALIAKHPGSFFASEAALVLAKSA
                     FDAGKLDEARKQLEWVMANGAEEHRGIARVRLAAILMAQKKFDEALKTLDGNKDEAFV
                     PLAADLRGDIMLAQGRLDEARAAYKLAIDKSSPRNPVRNIAETKLNALGGAQ"
     gene            1168..>1601
                     /locus_tag="VF309_07310"
     CDS             1168..>1601
                     /locus_tag="VF309_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7466430.1"
                     /translation="MKPLRIALLAAAALALAGCGSWSPLVALGFKTAPAHVPTPLAPI
                     NVQVTPRAVWTAHAGKSQGFSFRPVSQGGHVYAAAADGTVSVFDEDTGRVVAKFDAKK
                     KLSGGLEVGEAKIIAGTSKGEVIALDMNGKPLWTSPVGGEVIA"
BASE COUNT          280 a          520 c          589 g          212 t
ORIGIN      
        1 cctcgacgag gcgggagtcg cctatcgcgt gaacgcgcgc ctggtgcgcg ggctcgatta
       61 ctacaaccgc acggtcttcg agtggatctc gcccgagatc ggcgcgcaat cgaccctcgc
      121 cggaggcggc aggtacgacg cgctcttcga gcagctgggc ggcaagccca cgcccgcgtg
      181 cggcttcggc atgggaatcg agcgcgtgct cctcgcgtgg caggcggccg atggccatgc
      241 cgcatacgag cccgacgtct tcgtggtgca cgcgggcgag gcggccgaga tcgaggcgtg
      301 gaaggcctcg gagaagctgc gcgacgcggg gctcagcgtg atcctcgggg cgggcggcag
      361 cttcaagtcg cagatgaaga aagccgacgc gagcggcgcg cgctacgcgg tgatcctggg
      421 cgaggacgag gtgagcgccg ccaagttgac cgtgaaatcc ctgcgcggag cgggagagca
      481 gaagatgctc gcgcttcgcg aggcaatcga acacatcaga taaggcaatc ccatgtccgg
      541 atcgtacgat ttcgaagagc aggaacgcct cgcagagctg aaggcctggt gggaagacaa
      601 ccgctggtac gtggtgggcg ccgtggtcgc ggccgccgtc gcgttctcgg gttatcgcgg
      661 ctggatctac tggaaggcga gcaccgccga ggacgccgcg gcgatgtacc agccggtcga
      721 ggaggccttg aaggcgaatg acgcgaagaa ggtgcccgag acggcccagg cgctcatcgc
      781 gaagcatccc ggctccttct tcgcctcgga agccgccctc gtgctggcga agagcgcgtt
      841 cgacgcgggc aagctcgacg aggcgaggaa gcagctcgag tgggtgatgg cgaacggcgc
      901 cgaggagcac cgcggcatcg cgcgcgtgcg cctcgccgcg atcctcatgg cgcagaagaa
      961 attcgacgag gcgctgaaga ccctcgacgg caacaaggac gaggcgttcg tgccgctcgc
     1021 cgcggacctg cgcggcgaca tcatgctcgc gcaggggcgc ctcgacgagg cgcgcgcggc
     1081 ctacaagctc gcgatcgaca agtcgagccc gcgcaatccc gtgaggaaca tcgcggagac
     1141 gaagctgaac gccctgggag gcgcgcaatg aagccgcttc gcatcgcgct cctcgccgcc
     1201 gcggcgctgg cgctcgccgg ctgcggcagc tggagtccgc tggtcgccct cggcttcaag
     1261 acggcgcccg cccacgtgcc cacgcccctc gcgccgatca acgtgcaggt cacgccgcgc
     1321 gcggtctgga ccgcgcacgc cgggaagtcg cagggcttca gcttcaggcc cgtttcgcag
     1381 ggcgggcacg tctacgcggc ggcggccgac ggcaccgtct cggtcttcga cgaggacacc
     1441 ggccgcgtcg tcgcgaagtt cgacgcgaag aagaagctct cgggcggact cgaggtgggc
     1501 gaggcgaaga tcatcgccgg cacctcgaag ggcgaggtga tcgccctcga catgaacggc
     1561 aagcccctgt ggacgagccc cgtcgggggc gaggtgatcg c
//
LOCUS       DASVII010000559         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_421389,
            whole genome shotgun sequence.
ACCESSION   DASVII010000559 DASVII010000000
VERSION     DASVII010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421389"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VF309_07315"
     CDS             complement(<1..386)
                     /locus_tag="VF309_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7466431.1"
                     /translation="MSESLAIERQGPLGLITMNRPDRHNAFDDALIAELTEALRSMEA
                     EDGIRVIVLSGAGKSFSAVADLNWMKRMAGFSMDENRRDAMGLATLMRTLAHLRKPTL
                     ARVHGAAYGGGVGLVACCDVAVAAQSA"
     gene            complement(397..>2050)
                     /locus_tag="VF309_07320"
     CDS             complement(397..>2050)
                     /locus_tag="VF309_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7466432.1"
                     /translation="QARAGNRRRLKMPKIKSKIDPGSAAFKANVEHHRALAGDLRLHV
                     ARISEGGGSAAQQKHVARGKLLPRERVRALLDPGSPFLELSQLAAFGMYGDDAPSAGI
                     ITGIGRVEGREVVIVANDATVKGGTYYPMTVKKHLRAQEIALQNRLPCIYLVDSGGAF
                     LPLQDEVFPDKEHFGRIFYNQANLSALGVPQVAAVMGSCTAGGAYVPAMCDESIIVKN
                     QGTIFLGGPPLVKAATGEVVTAEDLGGGDVHSRTSGVTDHLADDDRHALAIAREIIAH
                     ASPLSSRASQRGNGPGEALDPLYPVEDLYGIIPADPKTPFDIREIIARLVDGSDFHEF
                     KALYGTTLVTGFARLHGHPVGILANNGILFSESALKGTHFIELCTQRGIPLLFLQNIT
                     GFMVGRKYEAGGIAKDGAKMVTAVSCAKVPKLTVILGGSFGAGNYGMCGRAFNPRFLW
                     TWPNARISVMGGEQAASVLATVRRDGLEAKGQQWSKADEDSFKNPIREQYERQGHPYY
                     ATARLWDDGVIDPADTRRVLGLALAATVNAPIEETRFGVFRM"
BASE COUNT          325 a          679 c          707 g          339 t
ORIGIN      
        1 gccgactgcg ccgccaccgc gacgtcgcag cacgccacga gccccacgcc gccgccgtag
       61 gccgcgccat gcacccgcgc gagcgtcggc ttgcgcaggt gggcgagggt gcgcatgagg
      121 gtggcaaggc ccatcgcgtc gcggcggttc tcatccatcg agaaacccgc catgcgcttc
      181 atccagttca ggtcggcgac cgcggagaat gacttgccgg cgccggacaa gacgatcacg
      241 cggatcccgt cctcggcctc catcgagcgc aaggcctcgg tgagctcggc gatcagcgca
      301 tcgtcgaacg cgttgtggcg gtcggggcgg ttcatggtga tgagcccgag ggggccctgt
      361 cgctcgatgg cgagggattc gctcactcga tttcctctac atgcggaaga cgccgaagcg
      421 cgtctcttcg atgggggcgt tcacggttgc ggcaagggcg aggccgagga ccctgcgcgt
      481 gtcggccggg tcgatgacgc cgtcgtccca gagccgcgcc gtcgcgtagt acggatggcc
      541 ctgcctctcg tattgctcgc ggatcggatt cttgaacgaa tcctcgtccg ccttcgacca
      601 ttgctggcct ttcgcctcga ggccgtcgcg ccgaacggtc gcgagcaccg aggccgcctg
      661 ctccccgccc atcaccgaga tccgggcatt gggccaggtc cagaggaagc gcggattgaa
      721 cgcgcgtccg cacatgccgt agtttcccgc gccgaacgag ccgcccagga tcacggtgag
      781 cttcggcacc ttcgcgcaag acacggccgt caccatcttc gcgccgtcct tggcgatgcc
      841 gccggcttcg tacttcctgc cgaccatgaa cccggtgatg ttctgcagga agagaagcgg
      901 aatgccgcgc tgcgtgcaaa gctcgatgaa gtgggtgccc ttcagggccg actccgaaaa
      961 caggatgccg ttgttggcga gaatgccgac ggggtggccg tgcaggcgcg cgaaccccgt
     1021 gacgagcgtc gttccataga gcgccttgaa ctcgtggaag tcggagccgt ccaccaggcg
     1081 cgcgatgatc tcgcggatgt cgaagggggt cttgggatcg gcggggatga ttccgtagag
     1141 gtcttcgaca gggtagaggg gatcgagcgc ctcacccggc ccattgccgc gttgcgacgc
     1201 ccgggacgac aggggcgacg catgggcgat gatctcccgc gcgatcgcga gcgcgtgccg
     1261 atcgtcgtcg gcaagatgat cggtgacgcc ggacgtgcgc gaatgcacgt cgcctccgcc
     1321 caagtcttcc gccgtgacca cctcgcccgt cgcggccttc accaacgggg gacccccgag
     1381 aaatatggtc ccctggttct tcacgatgat cgactcgtcg cacatcgcgg gaacataggc
     1441 gccgcccgcg gtgcacgagc ccatcaccgc ggcgacctgg ggcacgccga gcgcggagag
     1501 gttcgcctgg ttgtagaaga tgcgcccgaa atgctccttg tcgggaaaca cctcgtcctg
     1561 cagcggcagg aaggcgcccc cggaatcgac caggtagatg cagggcaggc gattctgcag
     1621 cgcgatttcc tgcgcgcgaa ggtgcttctt cacggtcatc gggtagtagg tgccgccctt
     1681 aaccgtggcg tcgttggcga cgatcacgac ttcccggccc tcgacgcggc cgatgccggt
     1741 gatgattccg gccgatggcg catcgtcgcc gtacatgccg aacgcggcga gctgggagag
     1801 ctcgaggaag ggcgagcccg gatcgaggag ggcgcgcacg cgctcccggg gaaggagctt
     1861 gccgcgggcc acgtgcttct gctgcgccgc ggagccgccg ccctcggaga ttcgcgcgac
     1921 gtgcaggcgc aggtcccccg cgagcgcgcg gtggtgctcc acgttcgcct tgaacgcggc
     1981 ggaccccgga tcgatcttcg acttgatttt cggcatcttc aggcggcgcc ggtttccagc
     2041 gcgcgcttgg
//
LOCUS       DASVII010000560         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_169383,
            whole genome shotgun sequence.
ACCESSION   DASVII010000560 DASVII010000000
VERSION     DASVII010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169383"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VF309_07325"
     CDS             <1..266
                     /locus_tag="VF309_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX7466433.1"
                     /translation="DPPQKATQSAIDAARACGWEVVSSDAPSGRIEATDTSLWFGFKD
                     DIVVRVRPDGAGSRVDVRSVSRVGKSDIGANAKRVRGFLARLA"
     gene            298..996
                     /locus_tag="VF309_07330"
     CDS             298..996
                     /locus_tag="VF309_07330"
                     /inference="COORDINATES: protein motif:HMM:NF006093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEX7466434.1"
                     /translation="MSKEQDIVATLEKVTTATITTILLKKGLRNVWMRGTRPLNPGQP
                     RLVGKAFTLRFVPAREDLATPESWAKPISTRAAIEAMPAGCIAVADAMGVTDAGIFGD
                     ILCARIAKRGVKALITDGVVRDVAGVRATGLPVWCQGAAAPPSVAGLTFVNWGEPIGC
                     GGVAVFPDDWIVVDDDGAVLIPAASIDDLVAAAPEQERLEGWIMGEVEKGASLPGLYP
                     PNAENKLRYEKTKA"
     gene            1064..>1598
                     /locus_tag="VF309_07335"
     CDS             1064..>1598
                     /locus_tag="VF309_07335"
                     /inference="COORDINATES: protein motif:HMM:NF040252.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466435.1"
                     /translation="MANPLLPRWTGPYGGVPPFDKVKVEHFKPALESAMAEELAEIES
                     IAANPAPPTFENTIAAMERAGRTLDRVDRVYSVFSSTMRSPAFREVEREMEPKLAQFR
                     DRIVQNERLFKRISAVYEARERSGLTPEQQRLVWLKYNDFARSGARLDAGAKKRLSEI
                     NERLASLYTEFSQNVLAD"
BASE COUNT          270 a          547 c          557 g          224 t
ORIGIN      
        1 aggatccgcc gcagaaggcg acccagagcg cgatcgatgc ggcgcgcgcg tgcggctggg
       61 aggtggtctc gagcgacgcc ccgtccgggc gcatcgaggc gaccgacacc tcgctctggt
      121 tcggcttcaa ggacgacatc gtcgttcggg tgcgtcccga cggggccggc agccgcgtcg
      181 acgtgcgcag cgtctcgcgc gtgggcaaga gcgacatcgg cgccaacgcc aagcgcgtgc
      241 gcgggttcct cgcgcggctc gcgtaagatc cgcgttcgcc cgtcacccgg aaaatccatg
      301 agcaaagagc aagacatcgt cgccacgctg gagaaggtga cgaccgccac catcaccacc
      361 atcctcctca agaagggcct gcgcaacgtg tggatgcgcg gcacgcgccc gttgaacccg
      421 gggcagccgc gcctggtcgg caaggctttc accctgcgct tcgtgccggc gcgcgaggac
      481 ctcgcgacgc ccgagtcatg ggccaagccg atctccacgc gcgccgcgat cgaggcgatg
      541 ccggcgggct gcatcgcggt ggccgacgcc atgggcgtca ccgatgccgg catcttcggc
      601 gacatcctct gcgcgcgcat cgccaagcgc ggcgtgaagg cgctcatcac cgacggcgtg
      661 gtgcgcgacg tggcgggcgt gcgcgcgacg ggcctgccgg tgtggtgcca gggcgccgcg
      721 gcgccgccat cggtcgcggg cctcaccttc gtgaactggg gcgagcccat cggctgcggc
      781 ggcgtggcgg tcttccccga cgactggatc gtggtcgacg acgacggcgc ggtgctgatc
      841 cccgcggcct ccatcgacga cctcgtggcc gccgcgccgg agcaggagcg tctcgagggg
      901 tggatcatgg gcgaggtgga gaagggcgca tcgctgcccg gcctctatcc gccgaacgcc
      961 gagaacaaac ttcggtatga aaagaccaag gcgtaagtgc agtagtattt gaaaagtcgc
     1021 gagagtccca caagggccga agcgcgaagc tccaggaggc agcatggcga acccacttct
     1081 cccccgctgg accggtccgt atggcggcgt ccctccgttc gacaaggtga aggtcgagca
     1141 cttcaagccg gcgctcgaat cggccatggc cgaggagctc gccgagatcg agtcgatcgc
     1201 cgcgaatccc gcgccgccca cgttcgagaa caccatcgcc gccatggagc gcgccgggcg
     1261 cacgctcgat cgtgtcgacc gcgtatacag cgtcttcagc tcgacgatgc gctcgcccgc
     1321 cttccgcgag gtggagcgcg agatggagcc gaagctcgcg cagttccgcg atcgcatcgt
     1381 gcagaacgag cggctcttca agcgcatctc ggcggtgtac gaggcgcgcg agcgctccgg
     1441 gctcacgccc gagcagcagc gcctggtatg gctcaagtac aacgatttcg cgaggagcgg
     1501 cgcgcggctc gatgccggcg cgaagaagcg cctgtccgag atcaacgagc gcctcgcatc
     1561 gctctacacc gagttcagcc agaacgtgct cgccgacg
//
LOCUS       DASVII010000561         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_349679,
            whole genome shotgun sequence.
ACCESSION   DASVII010000561 DASVII010000000
VERSION     DASVII010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349679"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1955
                     /locus_tag="VF309_07340"
     CDS             237..1955
                     /locus_tag="VF309_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7466436.1"
                     /translation="MRIQISALALAALSISTVACQGDPNDPMTWAKQLKNLRVQKEAL
                     DHLANMDVEKARPAVPAIIELYNDAKRPEHLEALARYLDPQTKPIMIAALDYTDDDFD
                     RATIASGVLGDMKATDAVDPLIKAVEKQLPVKSRANNARIAAMRALVKIGDKRAVPTL
                     MKVLTTSADDQDFLLNQKAALGLAEFRDPQAIPALIKGMFMTGRGTGIFQECRLALVR
                     IGTPAVAPIVELLQEKNADVQEMASKLKFDEVTPGVVPHKAAILLGDLRAKKAVPALI
                     GRLHQKARGDEHRSALIALGYIATPSAVDAILSVLKDGKADPAERASAADGLYLSGDR
                     RAVPTLLEIAKSGYVTTDGQKASDLRASAAIALARIAGKETYEAFNALVEKETAAQGV
                     FGMALDRMQVAKECDQDLACYGKKLNDPSWERSEKAAFAIAFSGNAKQGLPLLLAAMK
                     PITTMVPERYPVHQAILFALARLGTRECAECINKLDKQIERDEKATRIPGAKDLLGET
                     RVTEAIIQNRGASDAASDAAGAKEPIATAEPTDAGGGKGKAGKAGKSKAKGGKQAKAG
                     GKKKHK"
BASE COUNT          368 a          710 c          638 g          251 t
ORIGIN      
        1 cagaacgtca acttcgccgt catcccgaag accaagggcg gcggcgcccc cgcagccccc
       61 taagccccgc ccccccgtcg aacaggcacg gccggctctc tgcagggggc cggccgtttg
      121 tctttctgac ggtgggcggc tcgctgccgc tcgcgccccc cgggcccctc tcgcttcgcc
      181 cagcaaagct gggcttcgct cgccttcgta gttcgccaac cgtcaggaga aagctcatgc
      241 gtatccagat cagcgcgctc gcactcgcgg ccctttcgat atcgaccgta gcctgtcagg
      301 gcgatcccaa cgatccgatg acgtgggcca agcagctcaa gaacctgcgc gtccagaagg
      361 aggcgctcga tcacctggcc aacatggacg tcgagaaggc ccgcccggcc gtccccgcga
      421 tcatcgagct ctacaacgac gccaagcgcc ccgagcacct cgaggcactc gcccgctacc
      481 tcgacccgca gaccaagccg atcatgatcg cggcgctcga ctacacggac gacgacttcg
      541 accgcgcgac gatcgcgtcg ggcgtgctcg gcgacatgaa ggccaccgac gcggtcgatc
      601 ccttgatcaa ggcggtcgaa aagcagctgc cggtcaagtc gcgcgcgaac aacgcccgca
      661 tcgcggcgat gcgggcgctg gtcaagatcg gcgacaagcg cgccgtgccg acgctgatga
      721 aggtcctgac cacgtcggcc gacgaccagg acttcctcct caaccagaag gccgccctgg
      781 gactggccga gttccgcgac ccccaggcga tcccggccct catcaagggc atgttcatga
      841 ccggccgcgg gaccggcatc ttccaggagt gccgactggc gctggtccgc atcggcacgc
      901 ccgcggtcgc tccgatcgtc gagctccttc aggagaagaa cgccgacgtc caggagatgg
      961 ccagcaagtt gaagttcgac gaggtgaccc cgggcgtggt gccgcacaag gcggcgatcc
     1021 tcctcggtga tctccgggcg aagaaggccg ttcccgccct catcgggcgc ctgcaccaga
     1081 aggcccgcgg cgacgagcac cgctcggcgc tgatcgcgct cggctacatc gcgacccctt
     1141 cggccgtcga cgcgatcctc tccgttctca aggacggcaa ggcggatccg gccgagcgcg
     1201 cctcggccgc cgacggcctc tatctgtcgg gcgatcggcg cgcggtgccg acactgctcg
     1261 agatcgcgaa gtcgggctac gtgaccaccg acggacagaa ggcctccgac ctgcgcgcca
     1321 gcgcggcgat cgccctcgcc cgcatcgcgg gcaaggagac ctacgaggcg ttcaacgccc
     1381 tcgtcgagaa ggagaccgcc gcccagggcg tgttcggcat ggcgctcgac cgcatgcagg
     1441 tcgcgaagga gtgcgaccag gatctcgcct gctatggcaa gaagctcaac gacccgtcgt
     1501 gggagcggtc cgaaaaggcg gccttcgcga tcgcgttctc cggcaacgcc aaacagggcc
     1561 tgccgctgct gctcgcggcg atgaagccga tcaccaccat ggtccccgag cgctacccgg
     1621 tccaccaggc gatcctgttc gccctcgccc gcctcggcac acgcgagtgc gcggagtgca
     1681 tcaacaagct cgataagcag atcgagcggg acgagaaggc gacccgcatc ccgggtgcga
     1741 aggatctcct tggcgagacc cgcgtcacgg aggcgatcat ccagaaccgc ggcgccagcg
     1801 acgcggcgtc cgacgcggcc ggggcgaagg aaccgatcgc gaccgccgag ccaaccgacg
     1861 cgggcggagg gaagggcaag gcgggcaagg cgggcaagag caaggcaaag ggcggcaagc
     1921 aggccaaggc aggcgggaag aagaagcaca agtagcaccc gccccac
//
LOCUS       DASVII010000562         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_457239,
            whole genome shotgun sequence.
ACCESSION   DASVII010000562 DASVII010000000
VERSION     DASVII010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457239"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VF309_07345"
     CDS             <1..480
                     /locus_tag="VF309_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7466437.1"
                     /translation="TRYPIGLFHAWSYVDFKLQVTVYPKPDPLAGTPPAQSRSASEEG
                     IPIAGDEEFNMLRAYRPGDAPKLIAWKALAREQGLLTKEFSATASSELWLDWDEAHAP
                     DLEARLSVLTHWVLQAERFGQSFGLRIPGTVIAPARGEAHRTRCLEALALFHMPGAT"
     gene            477..>1703
                     /locus_tag="VF309_07350"
     CDS             477..>1703
                     /locus_tag="VF309_07350"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HEX7466438.1"
                     /translation="MKAAPSPHLDIRNVLWLLAAMVFVVAPHLLRMPYWVGTFFLIVV
                     AWRAWIAWAAMRLPSPWVTRGLTLAALLATWLQYGRIFGREPGVTLLIVMAALKLLEM
                     RNQREVTLSIYLGFFLVLTNFLFSQTIPLGLYMLVCVWVFIATLVGFNRIGTRPTLAE
                     RLRPAGALLVQALPLMVAFFILFPRAQGPLWALPQDTRSGTSGLSETMSPGNIANLIK
                     SDAVAFRVQFDGDRPPYSTLYWRGPVMVDFDGRTWSAARAAPETVVSFAQRQRRVSYT
                     VTLEPHQKTWLFALDAPAVLPTGSYMLSDLQLRDRMPVVERKRYEMSSYLDYKYGERS
                     SQGALQSSLRFPEDRNPRTVALARKWARESADPQAILAKVFQFYNREFSYTLEPPTLD
                     PRDPFDDFLFVSKQGFC"
BASE COUNT          238 a          614 c          556 g          295 t
ORIGIN      
        1 acgcgctacc cgatcggcct cttccacgcc tggtcgtacg tggacttcaa gctccaggtg
       61 accgtgtatc cgaagcccga tccgctcgcg ggcacgccgc ccgcgcaatc gcgcagcgcc
      121 tccgaggaag gcatcccgat cgcgggggac gaggaattca acatgctgcg cgcctaccgc
      181 cccggggacg cgccgaagct catcgcgtgg aaggccctcg cgcgcgagca gggcctgctc
      241 accaaggaat tcagcgcgac cgcttcgagc gagctgtggc tcgactggga cgaggcccat
      301 gcgccggacc tcgaggcgcg cctgtcggtg ctcacgcact gggtgctgca ggccgagcgt
      361 ttcggccaga gcttcgggct gcgcatcccc ggcacggtca tcgcgcccgc gcgcggcgag
      421 gcccaccgca cgcgctgcct cgaagcgctc gcgctcttcc acatgccggg agcgacgtga
      481 aggccgcgcc ctcgccgcat ctcgacatcc gcaatgtgct gtggctgctt gccgccatgg
      541 tgttcgtggt cgcgccccat ctcctgcgca tgccgtattg ggtgggcacg ttcttcctca
      601 tcgtggtcgc gtggcgcgca tggatcgcgt gggcggcgat gcgcctgccg tcgccctggg
      661 tgacgcgcgg gctcacgctc gccgcgctcc tggcgacatg gctgcagtac ggccgcatct
      721 tcgggcgcga gccgggggtg acgctcctca tcgtgatggc ggcgttgaag ctcctcgaga
      781 tgcgcaacca gcgcgaggtc acgctcagca tctacctcgg cttcttcctc gtcctcacca
      841 atttcctctt ctcgcagacg atcccgctcg gcctctacat gctcgtctgc gtctgggtct
      901 tcatcgcgac gctggtgggg ttcaaccgca tcggcacccg gcccacgctc gccgagcgac
      961 tgcgcccggc cggcgcgctg ctggtgcagg cgctgccgct gatggtggcc ttcttcatac
     1021 tctttccgcg cgcgcagggt ccgctctggg ccctgcccca ggacacgcgc tcgggcacct
     1081 cgggcctttc ggagacgatg agccccggca acatcgcgaa cctcatcaag tccgacgcgg
     1141 tcgccttccg cgtgcagttc gacggcgacc ggccgcccta ctcgacactc tattggcgcg
     1201 gcccggtgat ggtcgatttc gacggcagga cgtggagcgc ggcgcgcgcc gcgccggaga
     1261 ccgtggtgag cttcgcgcag cgccagcggc gcgtgagcta cacggtgacg ctcgagccgc
     1321 accagaagac gtggctgttc gcgctcgacg cgccggcggt gctgccgacg ggatcgtaca
     1381 tgctctccga cctgcagctg agggaccgga tgccggtggt cgagcgcaag cgctacgaga
     1441 tgagctcgta cctcgactac aagtacggcg agcgctcgtc gcagggagcg ctgcagtcgt
     1501 cgctgcgctt tcccgaggac cgcaacccgc gcaccgtggc cctcgcgcgg aaatgggcgc
     1561 gcgagtccgc ggatccgcag gccatcctcg ccaaggtctt ccagttctac aaccgcgagt
     1621 tttcctacac gctcgagccg ccgacgctcg atccgcgcga tcccttcgac gattttctct
     1681 tcgtaagcaa gcagggattc tgc
//
LOCUS       DASVII010000563         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_385731,
            whole genome shotgun sequence.
ACCESSION   DASVII010000563 DASVII010000000
VERSION     DASVII010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385731"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1739
                     /locus_tag="VF309_07355"
     CDS             24..1739
                     /locus_tag="VF309_07355"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX7466439.1"
                     /translation="MPAPADVAVPDHQKTAVTPAAGASAKTQSAAELLVKCLENEGVD
                     YIFGIPGEENIDVMDALLGSSITFVTCHHEQGAAFMADVYGRLTGRAGVCMSTLGPGA
                     TNLITGVADANMDRAPLVAIAGQGATTRLHKESHQILDLVNMFKPITKYSTQVLDPGI
                     TPEIVRKAFKVAQTEKPGAAFIDFPENIAEMKVDKKPIPVQSAYSPNAPDAKIDYAAK
                     LISEAKNPVILAGNGVIRQEAADSLVSFAEVLKIPVANTFMAKGIMPFTNELCLGTIG
                     LKARDLPWFAFEQADCVVCVGYDMVEYHPDMWNPNNDKTIIHIDALPAEVDANYIVKV
                     GVLGDIGHSLRGIALKAKPRKEPAFKDVRKSIVEDRREYANDTAFPVKPQKIVWDVRE
                     VLGPEDIAISDVGAHKMWMSRMYRAERPNTCIISNGFAAMGIAVPGAIAAKLAYPGKK
                     VIAVTGDAGFMMNSQEIETAMRMKTPIVILIWNDSEYGLITWHQLRHFGRPAYIDFKN
                     PDFVKYAESFGAAGFRVSRTEDLVPTMKKALAMDTVVIIDCPVDYRENMKLTERLKKI
                     GEGKR"
     gene            1736..>2065
                     /locus_tag="VF309_07360"
     CDS             1736..>2065
                     /locus_tag="VF309_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7466440.1"
                     /translation="MKIRAAVLNAMGAALPYAKSKPLTIETIELKPPGPGEILVKMGA
                     AGLCHSDLSVINGDRPRPTPMALGHEAAGTVQELGPGVTDLKVGDHVVLVFIPSCGHC
                     NPCAEGRP"
BASE COUNT          409 a          679 c          660 g          317 t
ORIGIN      
        1 cccgctcgac aggacaatcc cctatgccag cccccgcgga tgtcgccgtt cccgatcatc
       61 aaaagaccgc cgttaccccc gccgcaggcg ccagcgccaa gacgcagagc gccgccgagc
      121 tcctcgtcaa atgcctcgag aacgagggcg tcgattacat cttcggcatc cccggcgagg
      181 agaacatcga cgtgatggac gcgctcctcg gcagctcgat cacgttcgtg acctgccacc
      241 atgagcaggg cgcggccttc atggccgatg tctacgggcg gctcaccggc cgcgcgggcg
      301 tctgcatgtc gacgctgggt cctggcgcca ccaacctcat cacgggcgtg gccgatgcca
      361 acatggaccg cgcgccgctc gtcgccatcg cgggacaggg cgcgacgacg cgccttcaca
      421 aggagagcca ccagatcctc gacctggtga acatgttcaa gccgatcacg aagtacagca
      481 cccaggtgct cgatccgggc atcacgccgg agatcgtgag gaaggcgttc aaggtcgcgc
      541 agaccgagaa gccgggcgcc gccttcatcg actttcccga gaacatcgcc gagatgaagg
      601 tcgacaagaa gcccatcccg gtgcagtcgg cctacagccc caacgcgccc gacgcgaaga
      661 tcgactacgc cgcgaagctc atctcggagg cgaagaaccc ggtgatcctc gcgggcaacg
      721 gcgtgatccg ccaggaggcc gccgattccc tcgtgagctt cgccgaagtg ctgaagatcc
      781 ccgtggccaa taccttcatg gcgaagggca tcatgccctt caccaacgag ctgtgcctcg
      841 gcaccatcgg cctcaaggcg cgcgacctcc cgtggttcgc tttcgagcag gcggactgcg
      901 tcgtctgcgt cggttacgac atggtcgagt accaccccga catgtggaac ccgaacaacg
      961 acaagaccat catccacatc gatgcgcttc ccgccgaggt ggacgcgaac tacatcgtga
     1021 aggtgggcgt gctgggcgac atcgggcatt cgctgcgcgg catcgcgctg aaggcgaagc
     1081 cgcgcaagga gcccgccttc aaggacgtgc gcaagtcgat cgtcgaggac cgccgcgaat
     1141 acgcgaacga caccgccttc ccggtgaagc cgcagaagat cgtctgggac gtgcgcgagg
     1201 tgctcgggcc cgaggacatc gcgatctcgg acgtgggcgc ccacaagatg tggatgtcgc
     1261 gcatgtaccg cgcggagcgg cccaatactt gcatcatctc caacggcttc gcggccatgg
     1321 ggatcgcggt gccgggagcg atcgcggcga agctcgccta tcccgggaag aaagtgattg
     1381 cggtcaccgg agacgcgggc ttcatgatga attcccagga gatcgagacg gcgatgcgca
     1441 tgaagacgcc catcgtgatc ctcatctgga acgattccga gtacggactc atcacctggc
     1501 accagctgcg tcacttcggg cgccccgcgt atatcgattt caagaatccc gacttcgtga
     1561 agtacgcgga gagcttcggc gcggcgggtt tccgcgtctc gcggaccgag gacctggtgc
     1621 cgacgatgaa gaaggcgctc gcgatggaca cggtggtgat catcgattgc cccgtggact
     1681 atcgcgagaa catgaagctc accgagagat tgaagaagat tggagaaggg aagcgatgaa
     1741 gatccgcgca gccgtcctca acgcgatggg ggcggcgctg ccctacgcga aatcgaagcc
     1801 cctcacgatc gagacgatcg agctgaagcc cccggggccc ggcgagatcc tcgtgaagat
     1861 gggcgccgcg gggctctgcc attcggacct gtcggtcatc aacggcgatc gcccgcgccc
     1921 cacgccgatg gcgctgggcc acgaggcggc ggggacggtg caggagctcg gtcccggagt
     1981 gaccgatctc aaggtcggcg atcatgtcgt cctcgtgttc atcccgagct gcggccactg
     2041 caatccgtgc gccgagggcc ggccc
//
LOCUS       DASVII010000564         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_421477,
            whole genome shotgun sequence.
ACCESSION   DASVII010000564 DASVII010000000
VERSION     DASVII010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421477"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..840)
                     /locus_tag="VF309_07365"
     CDS             complement(205..840)
                     /locus_tag="VF309_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7466441.1"
                     /translation="MLAIHDLPLFLASAVLLNLTPGPDTLFILIQAAKGWRPGMLAAL
                     GIGTGCLVHILAATIGLSALLFASATAFALVKGIGACYLIWMGLTLLLARREPALASG
                     APIDAPDGKKIFVRGALTNALNPKVAMFFLAFLPQFVNSAAPRRTLSFLVLGCLFNLT
                     GTAWNLTLARLASGAVARRPAALKVAAGFSRGVGALFVGLGLKLALAREPP"
     gene            complement(879..>1595)
                     /locus_tag="VF309_07370"
     CDS             complement(879..>1595)
                     /locus_tag="VF309_07370"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7466442.1"
                     /translation="IEDALDEFFPGVPVVRIDRDTVRGRGEMEAALARVEGGDARILI
                     GTQMLTKGHDFPDVTLVVVLNADQGLFGTDFRSSERLAQSIVQVAGRAGRASRPGEVL
                     IQTACPEHPLLVRLLEGGYSAFAAGALEERAQARWPPFSHLAVLRAEAKERARALAFL
                     EGVRRLALGLGNRGVELLGPAPAAMERRAGRFRAQLLIEAGRRPALKRLLDELVPLIE
                     ALTEARRVRWSIDVDPLEVA"
BASE COUNT          253 a          536 c          609 g          197 t
ORIGIN      
        1 ttactcgcgt agtcgccgtg cttggggtcg cgggtgcgct cgatctcggg cggaagcgtc
       61 agggcctcct cgctcaaggt accgggcggc agcgcggcga gcgccgcgct cacgagtcgt
      121 tcgatggtcg ctttcaaggc agatccaggc ggggtgggcg ccggcggcgc agcccaagtc
      181 taccggaacg gggcggggcg gaccctaggg cggctcgcgc gcgagcgcga gcttgagccc
      241 gaggccgacg aagagcgcgc cgacgccgcg gctgaacccg gccgcgactt tgagcgccgc
      301 ggggcggcgc gcgacggcgc ccgaggcgag acgcgcgagg gtcaggttcc aggcggtgcc
      361 ggtcaggttg aagaggcacc cgagcacgag gaacgacagc gtccgcctgg gagcggcgct
      421 attgacgaac tgcggcagga acgcgaggaa gaacatcgcg accttggggt tcagcgcgtt
      481 cgtgagcgcc ccgcgcacga agatcttctt cccgtccggc gcgtcgatcg gcgctccgga
      541 ggcgagggcg ggctcacgcc gcgcgaggag gagcgtgagt cccatccaga tgagatagca
      601 ggcaccgatg cccttcacga gcgcgaacgc ggtcgcggat gcgaagagga gcgcggagag
      661 cccgatcgtc gccgcgagga tgtgcacgag gcagcccgtg ccgatgccaa gtgccgccag
      721 cattcccggg cgccacccct tcgccgcctg gatcaggatg aaaagcgtat cgggccccgg
      781 tgtcaggttg aggagcacgg cggacgcgag gaagagcggc aggtcgtgga tggcgagcac
      841 gatgtgagcc tagcgcgcca gtcggccggc gtcagcggct aggcgacctc gagcgggtcg
      901 acgtcgatcg accagcgcac gcgcctcgcc tcggtgagcg cctcgatgag cggcacgagt
      961 tcatcgagca gccgcttgag cgccgggcgt cgtcccgcct cgatgaggag ttgcgcccgg
     1021 aagcgccccg cgcggcgttc catcgcggcg ggcgcgggac ccaaaagctc gacgccgcgg
     1081 ttgccgagcc caagcgcgag gcgccgcacg ccttcgagga aggcgagtgc gcgggcgcgc
     1141 tccttggctt cggcgcgcag caccgcgagg tgactgaacg gcggccagcg cgcctgggca
     1201 cgctcctcga gcgcgccggc cgcaaaggcc gagtagccgc cctcgaggag gcgcacgagg
     1261 agcggatgct cggggcaggc ggtctggatc agcacctcgc ccgggcggct cgcgcgcccg
     1321 gcgcgtccgg cgacctgcac gatgctctgc gcgaggcgct cgctcgagcg aaagtccgtg
     1381 ccgaagagcc cctggtcggc attgagaacg acgacgagcg tgacgtccgg gaaatcgtgc
     1441 cccttcgtca gcatctgcgt gccgataagg atgcgcgcat cgccgccctc gacgcgtgcg
     1501 agcgccgcct ccatctcgcc gcggccgcgc acggtgtccc ggtcgatgcg gacgaccggc
     1561 acgcccggga agaactcatc gagcgcatcc tcgat
//
LOCUS       DASVII010000565         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_637062,
            whole genome shotgun sequence.
ACCESSION   DASVII010000565 DASVII010000000
VERSION     DASVII010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637062"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF309_07375"
     CDS             complement(<1..950)
                     /locus_tag="VF309_07375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7466443.1"
                     /translation="MSETRRIGKYEVRDLVGKGAGSAVYRGADGSKMVALKLMNRATV
                     PAQALARLQQNAATFARVRHPAIATFIEAIEADSVLCIVSELAEGESLASRMKDGAPY
                     DLRATWEIARQLLEGIESAHMRGVFHGNLKLTNLIVDRESRLKVTDLAAFGLGELTPN
                     AYIAPEQLGGHAPDARSDLYQVGAIVYHLVAGRAPFSGTREEIAHRVRQERPTDPSSY
                     SPKIAWQLDWVIQRALSKEPMDRFGSAREFLDGLRLGLQDSIGSPLPVPAPPPAAEAK
                     PAAAREAAKAAPQAKAAEPAKSAAPAAPAKVAAPATPPKPV"
     gene            complement(947..>1639)
                     /locus_tag="VF309_07380"
     CDS             complement(947..>1639)
                     /locus_tag="VF309_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466444.1"
                     /translation="ILRRASPDAEELQAGFNSLETRLERLRKEHVVEDLDALTKDGLH
                     GIEQIVDLVANLRNFSRLDRSKVASFNVNEGVRATLLIAKPTLRKIDVERHLGDIPSI
                     TCSPSQVNQVLLNLVTNSAQAMDKPRGHITVTTRAIGTSAIAIEVEDNGRGIVTEALP
                     RIVDPFYTTKEPGKGTGLGLSIAYKIVAQHGGRIEARSTPGVGTVVTVTLPVEPPAEL
                     AAFAEEEAARVA"
BASE COUNT          224 a          532 c          600 g          283 t
ORIGIN      
        1 acaggcttcg gaggcgtagc aggtgcggcg accttcgcgg gcgcggccgg cgcggcggac
       61 ttcgcgggtt cagcggcctt ggcttgcggt gccgccttcg ccgcttcgcg cgccgcagcg
      121 ggtttcgcct cggcagcggg cggcggcgcg ggaacgggca gtggcgagcc gatcgaatcc
      181 tgcaggccga ggcgcaggcc gtcgaggaat tcgcgggccg agccgaagcg atccatcggc
      241 tccttggaaa gcgcgcgctg gatcacccag tcgagctgcc atgcgatctt cggcgagtat
      301 gacgacggat cggtaggacg ctcctgccgc acgcgatggg cgatctcttc gcgcgtgccg
      361 gagaatggcg cccgtcccgc gacgagatgg tagacgatgg cgccgacctg gtagagatcg
      421 ctgcgcgcgt cgggcgcgtg gccgcccagc tgctcgggcg cgatgtacgc gttcggcgtg
      481 agctcgccga ggccgaaggc ggcgaggtcc gtgaccttca ggcgcgattc cctgtcgacg
      541 atcaggttgg tcagcttgag gttgccgtgg aacacgcccc tcatgtgcgc cgattcgatt
      601 ccctcgagga gctggcgcgc gatctcccac gtcgccctga ggtcgtaggg ggcgccgtcc
      661 ttcatccgcg acgcgaggga ttcgccctcg gccagctccg agacgatgca aaggacgctg
      721 tcggcctcga tcgcttcgat gaaggtcgcg atcgcgggat ggcgcacgcg cgcgaaggtc
      781 gcggcattct gctgcaggcg cgcgagcgcc tgggcgggca cggtggcgcg gttcataagc
      841 ttcaacgcga ccatcttgct gccgtcggcg cccctgtaga cggccgagcc agcgcccttc
      901 ccgacgaggt cgcggacctc gtacttgccg atgcggcgcg tttcgctcat gccacgcgcg
      961 ccgcttcttc ttcggcgaat gccgcgagct ccgccggcgg ctcgaccggc agcgtcacgg
     1021 tcaccacggt tcccacgccg ggtgtggagc gcgcctcgat gcgcccgccg tgctgggcga
     1081 cgatcttgta ggcgatggag agccccagcc ccgtgccctt gccgggctcc ttggtggtgt
     1141 agaagggatc gacgatgcgc ggcagcgcct ccgtgacgat gccgcggccg ttgtcctcga
     1201 cctcgatcgc gatcgcgctg gtgccgatgg cgcgggtcgt gaccgtgatg tggccgcgcg
     1261 gcttgtccat cgcctgcgcc gagttggtga cgaggttgag cagcacctgg ttcacctgcg
     1321 agggcgagca cgtgatcgag ggaatgtcgc cgaggtggcg ctccacgtcg atcttgcgca
     1381 gcgtcggctt cgcgatgagg agggtcgcgc gaacgccctc gttcacgttg aagctcgcca
     1441 ccttgctgcg atcgaggcgc gagaagttgc gaaggttcgc cacgagatcg acgatctgct
     1501 cgatgccgtg gaggccgtcc ttcgtcaacg catcgagatc ctcgacgacg tgttccttgc
     1561 gaaggcgctc gaggcgcgtc tcgagcgagt tgaagcccgc ctgcagctct tcggcgtcgg
     1621 gtgaggcgcg gcgcaggat
//
LOCUS       DASVII010000566         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_385928,
            whole genome shotgun sequence.
ACCESSION   DASVII010000566 DASVII010000000
VERSION     DASVII010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385928"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VF309_07385"
     CDS             <1..723
                     /locus_tag="VF309_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466445.1"
                     /translation="AKTRNAKKASASDYTGAAAKAPLLRRFGRAADLGLSPREYATLK
                     RLQSPAVIQAFINAIPINHEVGGETVLSVREVLRQRRAHCIEGAMVAAAALWVNGDPP
                     LVMHLDCDESDYPHVVALFRRSGAWGAISKTNGAPLRYRDPIYRTLRELALSYFHEYS
                     NRRGHKTLRSYSVAFDLRRIDTGSWVTATKSCWETHDRLVELRHFPLISKRQERLLAK
                     RDAFERRASKLVEYPRPKPAPA"
     gene            complement(720..1286)
                     /locus_tag="VF309_07390"
     CDS             complement(720..1286)
                     /locus_tag="VF309_07390"
                     /inference="COORDINATES: protein motif:HMM:NF040504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEX7466446.1"
                     /translation="MIRIATSGDAEGILAIYRPIVRDTVISFELEPPDAAEIARRVEG
                     TLAKYPWLVYVDREGIAGYAYAAEHRERLAYQWSVDVSCYVHERARRRGIGKALYQAL
                     KRVLRAQGFCNAFAGIALPNAASVGLHEAVGFVALGKYRNVGFKLGAWRDSVWLQCAL
                     DEVPANPPPPLRLPELSMETVEAALRAP"
     gene            complement(1283..>1839)
                     /locus_tag="VF309_07395"
     CDS             complement(1283..>1839)
                     /locus_tag="VF309_07395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7466447.1"
                     /translation="RGDPIVMPAIAFRRLAVDDLQQVFLWLLQPHVARGYAPAPSSFM
                     EVVAKYGPRTREENVVRAYVFSVDGREAGYIQAYDLAHFPDYAAQVGSGAGVACMDLF
                     IGDEKLTGWGLGSRVLRQFVDEVVFAAEGIGACVAGPTEGNLASIRAFEKAGFARWKI
                     VKPPERESECVMRRERDRPPAGVP"
BASE COUNT          297 a          656 c          619 g          267 t
ORIGIN      
        1 gcgaagaccc gcaatgccaa gaaggcgagt gcaagcgatt acaccggcgc cgccgcgaag
       61 gcgccgctgc tgcgccgctt cggtcgcgcc gcggacctgg gcctttcgcc gcgcgaatac
      121 gcgacgctca agcgcttgca gtcgcccgcg gtgatccagg cgttcatcaa cgcgatcccg
      181 atcaaccacg aggtcggcgg agagacggtg ctctcggtgc gcgaggtcct gaggcagagg
      241 cgggcgcact gcatcgaagg cgcgatggtg gcggccgcgg ccctgtgggt gaacggcgat
      301 cctcccctcg tgatgcacct cgattgcgac gagagcgatt acccccacgt cgtggcgctc
      361 ttccgccgct cgggcgcctg gggggcgatc tccaagacca acggggctcc gctgcgctat
      421 cgcgatccga tctaccggac gctgcgcgag ctcgcgcttt cgtatttcca cgagtactcg
      481 aaccggcgcg gccacaagac gctgcgcagc tactcggttg cgttcgacct gcgccgcatc
      541 gacaccgggt cgtgggtgac cgcgacgaag tcctgctggg agacccacga ccgcctcgtg
      601 gagctgcgcc acttcccgct catctcgaaa cgccaggagc gcctgctcgc gaagcgcgac
      661 gcgttcgagc gcagggcgtc caagctcgtc gaataccccc ggccgaaacc ggcgcccgcc
      721 tagggcgcgc gcagcgccgc ttcgacggtc tccatcgaaa gctccggaag gcgcagcggc
      781 gggggcggat tcgcgggcac ctcgtccagc gcgcattgca gccacaccga gtcgcgccac
      841 gcgccgagct tgaatcccac gttgcggtac ttgccgagcg cgacgaagcc gaccgcctcg
      901 tgcaggccga cgctcgccgc gttcggaagc gcgatccccg cgaaggcgtt gcagaatccc
      961 tgcgcgcgca gcacgcgctt gagggcctgg tagagcgcct tgccgatgcc gcgccgccgc
     1021 gcgcgctcgt ggacgtagca cgagacgtcg acggaccact gatatgcgag cctctcgcga
     1081 tgctcggccg cataggcata ccccgcgatg ccctcgcgat ccacgtacac gagccacggg
     1141 tatttcgcga gcgtgccctc gacgcgccgc gcgatctcgg ccgcgtccgg aggctcgagc
     1201 tcgaaggaga tgacggtgtc gcgcacgatc ggccggtaga tcgcgaggat gccctcggcg
     1261 tcgccgctcg tcgcaatccg gatcatggga cgccggccgg cggccgatcg cgctcgcgcc
     1321 gcatcacgca ctcgctttcg cgctcgggcg gcttcacgat cttccagcgc gcgaagccgg
     1381 ccttctcgaa cgcgcggatg gaggcgagat tgccttcggt cggaccggcc acgcaggcgc
     1441 cgatgccctc cgcggcgaac accacctcat cgacgaattg gcgcagcacc cgcgagccga
     1501 gtccccaccc ggtgagcttc tcgtcgccga tgaacaggtc catgcacgcg acgccggcgc
     1561 cgctaccgac ctgcgccgcg tagtcgggga aatgcgcgag gtcgtacgcc tggatgtagc
     1621 ccgcctcacg gccgtcgacg ctgaagacat acgccctcac tacgttctct tcgcgcgtgc
     1681 gcggaccgta cttcgcgacg acttccatga aggagctcgg ggcgggcgca tagccgcggg
     1741 cgacgtgcgg ctggaggagc cacaggaaga cctgctggag gtcgtccacg gcgagacggc
     1801 gaaacgcgat ggcgggcatc actatcggat ctccgcgag
//
LOCUS       DASVII010000567         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_169737,
            whole genome shotgun sequence.
ACCESSION   DASVII010000567 DASVII010000000
VERSION     DASVII010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169737"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VF309_07400"
     CDS             <1..837
                     /locus_tag="VF309_07400"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX7466448.1"
                     /translation="SIMAKWCLAHHKESFLYTHFEDICAIMKAYDVSFSLGDGLRPGS
                     IYDANDEAQIAELKTLGELTKIAWKHDCQVMIEGPGHVPMQLIKENMDLELEHCHEAP
                     FYTLGPLTTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGLPDKEDVKDGIIT
                     YKIAAHAADLAKGHPGAQIRDNALSKARFEFRWDDQFNLGLDPDKAREFHDETLPQEG
                     AKLAHFCSMCGPHFCSMKITQDVREFAAKMGVSNEEALKKGMEVKSIEFVKQGAEIYA
                     KQ"
     gene            862..>1652
                     /locus_tag="VF309_07405"
     CDS             862..>1652
                     /locus_tag="VF309_07405"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7466449.1"
                     /translation="MDLHSLFNAFIQGVVEGLTEFLPVSSTGHLIIVSDLLGLNDEKG
                     KVFDIVIQLGAILAICWEYRARFAKAFSGLAGDPVQRRFAANLFVAFLPAAIVGLALQ
                     KQIKAYLFNPVSVAMALIVGAMVIFVVERWYAKHGAPRVTSVDEMDWKDALKVGLAQC
                     FSLIPGTSRSGATIMGGMLFGLSRQAATEFSFFLAVPIMFAASGYQMVKYRDLLSRDD
                     LATFAVGFVVSFAFALLAVKALIRYVAHHDFKAFAWYRIALGLAVL"
BASE COUNT          296 a          527 c          530 g          299 t
ORIGIN      
        1 tccatcatgg ccaaatggtg cctcgcgcat cataaggaga gcttcctcta cacgcatttc
       61 gaggatatct gcgcaatcat gaaggcctac gacgtttcct tctcgctcgg cgacgggttg
      121 cggcccggct cgatctacga cgccaacgac gaggcgcaga tcgcggagct gaagaccctg
      181 ggagagctca cgaagatcgc gtggaagcac gattgccagg tgatgatcga gggccccggc
      241 cacgtgccga tgcagctcat caaggagaac atggacctcg agctcgagca ttgccacgag
      301 gccccgttct acaccctggg ccctctgacc accgacatcg cgcccggcta tgaccacatc
      361 accagcggaa tcggcgcggc gcagatcggt tggtacggca ccgcgatgct ctgctacgtg
      421 acgcccaagg agcacctggg cctgcccgac aaggaggacg tgaaggacgg catcatcacg
      481 tacaagatcg ccgcccatgc cgccgatctc gccaagggac accctggcgc gcagatccgc
      541 gacaacgcgc tgtcgaaggc gaggttcgag ttccgctggg acgaccagtt caacctcggc
      601 ctcgatcccg acaaggcgcg ggagttccac gatgaaacgc ttccccagga aggcgccaag
      661 ctcgcgcact tctgctccat gtgcgggccg catttctgct cgatgaagat cacccaggac
      721 gtgcgggaat tcgccgcgaa gatgggcgtg tcgaacgagg aagcgctgaa gaaggggatg
      781 gaagtcaaat ccatcgagtt cgtgaagcag ggcgcggaga tctacgcgaa gcagtaacgc
      841 cccaaacgac gaggcttcgc gatggatctg cattcgctct tcaacgcgtt catccagggt
      901 gtggtcgagg ggctgacgga gttcctgccg gtctcctcga cgggccacct catcatcgtg
      961 tcggatctcc tcggcctcaa cgacgagaag ggcaaggtct tcgacatcgt gatccagctg
     1021 ggggcgatcc tcgccatctg ctgggagtat cgggcgcgct tcgcgaaggc cttctccggg
     1081 ctcgcgggcg atcccgtcca gcggcgcttc gcggcgaacc tcttcgtcgc cttccttccc
     1141 gcggcgatcg tgggcctcgc gctccagaag cagatcaagg cctacctctt caatccggtc
     1201 tcggtcgcga tggcgctcat cgtgggcgcg atggtgatct tcgtcgtcga gcgctggtac
     1261 gcgaagcacg gcgccccgcg cgtgacgagc gtggacgaga tggactggaa ggatgcgctc
     1321 aaggtgggcc tcgcgcagtg cttctcgctg atccccggaa cgtcgcgctc cggggcgacc
     1381 atcatgggcg ggatgctgtt cggattgtcg cggcaggccg cgacggagtt ctcgttcttc
     1441 ctcgcggtgc cgatcatgtt cgccgcgagc ggctaccaga tggtgaagta ccgcgacctt
     1501 ctgtcgcgcg acgacctcgc gaccttcgcc gtggggttcg tggtgagctt cgcgttcgcg
     1561 ctcctcgcgg tgaaggcgct gatccgctac gtcgcgcacc acgacttcaa ggcgttcgcg
     1621 tggtaccgga tcgccctggg cctcgcggtg ct
//
LOCUS       DASVII010000568         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_529672,
            whole genome shotgun sequence.
ACCESSION   DASVII010000568 DASVII010000000
VERSION     DASVII010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529672"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..772)
                     /locus_tag="VF309_07410"
     CDS             complement(131..772)
                     /locus_tag="VF309_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466450.1"
                     /translation="MHLLDRIETQLMSNRLPLVAISVAAVPYANTPVVLTLHWHGFVE
                     TRLSEVTDANVVAYQAVPSSALQVNERWDRFEQLENSVLDVAWELGSWDLARTEALPF
                     VRPGAPQQESVECMSAFGSIPMAIDGQPPVIAEVPDAGDLIETASRSGYVHWLFRPVR
                     GGVWAEVSEDTTLQEGGYRNPPCPYMPTPTRVGRTHRPRKVVYQFGRCTRLAR"
     gene            1024..1341
                     /locus_tag="VF309_07415"
     CDS             1024..1341
                     /locus_tag="VF309_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466451.1"
                     /translation="MPLLGNAEKGELERLILSRLTEHYAKNGPAALLEDGVRVIVNVD
                     GVAIDRGETLDPLAVVEVRSLFTALCYITGGTIALPPDSLQTLATEATSAAAQQINRI
                     AAG"
     gene            1344..>1519
                     /locus_tag="VF309_07420"
     CDS             1344..>1519
                     /locus_tag="VF309_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     aminotransferase BioA"
                     /protein_id="HEX7466452.1"
                     /translation="MSNREMVARSLAAVWHPCTQMKSHERFPLVPISRAQGVWLYDFE
                     GRRYLDAVSSWWVNL"
BASE COUNT          282 a          489 c          495 g          253 t
ORIGIN      
        1 taataatagg cccagcaagg cctgaattca agtggcgggg gcgtgaatat gattcgccgc
       61 gacgccacaa gagcgattgc ttacctctgc ggggtcgccc gaaacaagtc caaaaacaat
      121 gggttgccgg ttagcgggcg aggcgggtgc agcgcccgaa ctggtagacg actttgcgcg
      181 ggcggtgggt gcggcccacg cgcgtgggcg tcggcatgta cgggcatggg gggttgcgat
      241 agccgccctc ttgcagggtc gtgtcctccg aaacctccgc ccatacgccg cctcgcaccg
      301 gcctgaaaag ccagtgcacg taaccggagc gtgaggccgt ctcgatgagg tcgcccgcgt
      361 ccggcacttc ggcgatcacc ggcggctggc catcgatcgc catcggaatc gagccgaatg
      421 cgctcatgca ctcgaccgac tcctgctggg gcgcgccggg gcgaacgaag ggcaacgcct
      481 cggtgcgcgc cagatcccag gaacccagtt cccatgcgac gtccaatacc gagttctcga
      541 gctgctcgaa tcgatcccag cgctcgttca cctgcagcgc cgagctcggc accgcctggt
      601 aggcgacgac gttcgcatcg gtgacttcgg aaagccgtgt ctcgacgaag ccgtgccagt
      661 gaagggtgag caccacgggc gtgttggcgt agggcaccgc ggccaccgaa atcgcgacca
      721 gcggcagccg gttggacatc agttgcgtct cgatgcggtc gagaagatgc atttcgtcgc
      781 ctcccagtga gaatcgattc gttgccgagc tcttcaagcc tcctggatat gaagcaaaca
      841 acgtgcgcac tccgcttctc gcacgaaatc ggagggccat gttgctagca ctcgcgcccg
      901 atggatgcca agagcctctg gaaccggggt gcccagttgg gttaacgtcc ggtggcgaac
      961 atggttgaac gcgcggcggg gctagaatgg cgctgccccc ataggaagga acagctcaag
     1021 aacgtgccac tactgggaaa tgccgagaag ggtgaactcg agcgcctcat cctgtcgagg
     1081 ctcaccgagc actacgcgaa gaacggcccc gccgcgctcc tcgaggacgg cgtgcgcgtc
     1141 atcgtgaacg tcgacggcgt cgcgatcgac cgcggcgaga cgctcgaccc gcttgccgtg
     1201 gtcgaagtgc gctcgctctt taccgccctt tgctacatca cgggcggcac catcgcactt
     1261 cctcccgatt cgctgcaaac cctggcgacg gaagccacca gcgccgcagc ccagcagatc
     1321 aatcgcatcg cggcgggcta gcggtgtcca accgcgaaat ggtcgcgcgg agcctcgcgg
     1381 cggtgtggca tccgtgcacg cagatgaagt cccacgagcg cttcccgctc gtgccgatct
     1441 cccgcgcgca gggcgtgtgg ctctacgact tcgagggccg ccgctacctc gacgcggtga
     1501 gctcgtggtg ggtgaatct
//
LOCUS       DASVII010000569         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_134548,
            whole genome shotgun sequence.
ACCESSION   DASVII010000569 DASVII010000000
VERSION     DASVII010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134548"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF309_07425"
     CDS             complement(<1..563)
                     /locus_tag="VF309_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7466453.1"
                     /translation="MKVSLQWLKELVPVQAPVGELAKTLTMGGLEVEEITPVAGEFSG
                     IVVGYVKSVAPHPNADKLRVTEVDAGTGETLQIVCGAPNVAAGQKVPCALVGAKLPGL
                     EIRKAKLRGVESSGMLCSARELGLSEDHGGLLVLDADAPVGRDVREVLGLDDVYFTVK
                     LTPNRGDCLSMFGIARDLAAMVKMPLDS"
     gene            complement(560..1561)
                     /gene="pheS"
                     /locus_tag="VF309_07430"
     CDS             complement(560..1561)
                     /gene="pheS"
                     /locus_tag="VF309_07430"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7466454.1"
                     /translation="MKELEQIIAAAREEFAAAASVADLEQAKSRFLGKSGSLTGLLKG
                     LGRLSADERPQAGATINEGKARVEGLLRARLDEILARELEGRLAGEAIDVTLPGRGQG
                     PGGLHPIARVQEQVERLFRTMGFSVADGPEIEDDEHNFTALRMFPDHPSRSMQDTFYI
                     EGTDKVLRTQTSPVQIRYMKSHAPPIRIICPGRVYRKDHDATHSPMFHQVEGLWVEEG
                     VSLADLKGTVTQFCRAFFERDDIGLRFRPSYFPFVEPGVEIDMEWSRKDGEVTYLEIA
                     GAGVVHPDVLRNGGIDPEKYSGFAFGMGLDRLAMLKYGVNDLRLFFENDLKFLAQFR"
     gene            complement(1617..>1735)
                     /gene="rplT"
                     /locus_tag="VF309_07435"
     CDS             complement(1617..>1735)
                     /gene="rplT"
                     /locus_tag="VF309_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299089.1"
                     /note="binds directly to 23S ribosomal RNA prior to in
                     vitro assembly of the 50S ribosomal subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7466455.1"
                     /translation="GLKKAGIDVDRKILADLAVFDKAAFGRIVEQAKASLGA"
BASE COUNT          286 a          606 c          549 g          294 t
ORIGIN      
        1 gaatcgagcg gcatcttcac catcgccgcg agatcgcgag cgatgccgaa catggagagg
       61 caatcgccgc ggttgggcgt gagcttcacg gtgaaataaa cgtcgtcgag cccgagaacc
      121 tcgcgcacat cgcggccgac cggcgcatcg gcgtcgagca ccaggaggcc cccatgatcc
      181 tcggaaagcc ccagctcgcg ggccgaacag agcatgccgc tcgattcgac gccccgcagc
      241 ttggctttcc tgatctcgag gccgggcagt ttcgcgccga ccagcgcgca gggaaccttc
      301 tgccccgcgg cgacattggg ggcgccgcaa acgatctgca gcgtctcgcc ggtgcccgcg
      361 tcgacctcgg tcacgcgcaa cttgtcggcg ttcggatgcg gcgcgacgct cttcacgtac
      421 ccgaccacga tgccagagaa ttcgcccgcg accggcgtga tctcctcgac ctcgaggccc
      481 cccatggtga gcgtcttcgc aagctcgccc acgggcgcct gcaccggcac cagctccttc
      541 agccactgca gcgagacctt catcggaatt gcgccaggaa cttgaggtcg ttctcgaaga
      601 agaggcgcag gtcgttcacg ccgtatttca gcatcgcgag gcgatcgagc cccatgccga
      661 acgcgaagcc cgagtacttc tccgggtcga tgccgccgtt gcgcagcacg tcggggtgca
      721 ccacgcccgc gcccgcgatc tcgaggtagg tcacctcgcc gtccttgcgc gaccattcca
      781 tgtcgatctc gacgccgggc tcgacgaacg ggaaatacga gggccggaag cgcagcccga
      841 tgtcgtcgcg ctcgaagaac gcacggcaga attgcgtcac cgtccccttc aggtccgcga
      901 ggctcacgcc ctcttcgacc cacaacccct ccacctggtg gaacatcggc gagtgcgtcg
      961 cgtcgtggtc cttgcgatag acgcggcccg ggcaaatgat gcggatcggc ggcgcgtgcg
     1021 acttcatgta ccgaatctgc acgggcgagg tctgcgtgcg caggaccttg tcggtgccct
     1081 cgatgtagaa cgtgtcctgc atgctgcgcg acggatggtc gggaaacatg cgcaacgccg
     1141 tgaagttgtg ctcgtcgtcc tcgatctccg ggccgtcggc caccgagaac cccatggtgc
     1201 gaaacagcct ctcgacctgc tcctggacgc gcgcgatcgg atgcagccca cccggcccct
     1261 ggccgcgccc gggaagcgtc acgtcgatcg cctcgccggc gagcctgccc tcgagctcgc
     1321 gcgcgaggat ctcgtcgaga cgcgcgcgca ggaggccctc gacccgcgcc ttgccctcgt
     1381 tgatcgttgc gcccgcctgc gggcgctcgt cggccgaaag cctgccgagg cccttcagga
     1441 gcccggtgag cgaacccgac ttcccgagga agcgcgactt ggcctgctcg agatcggcga
     1501 cgcttgcggc ggcggcgaac tcttcgcggg ctgcggcgat gatttgttcg agttctttca
     1561 tcaggtcaaa aaagaaaagg ggctctcgcg agcccccttg tctgctttac gtcgtctcag
     1621 gcaccgaggc tcgctttggc ctgttcgacg atcctgccga atgcggcctt gtcgaacacc
     1681 gcgaggtcgg cgaggatctt gcggtccacg tcgatgccgg ccttcttcag gcccg
//
LOCUS       DASVII010000570         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_493792,
            whole genome shotgun sequence.
ACCESSION   DASVII010000570 DASVII010000000
VERSION     DASVII010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493792"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VF309_07440"
     CDS             complement(<1..804)
                     /locus_tag="VF309_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7466456.1"
                     /translation="MTTRRDVLLGAAAAALLGGSRLARAKAAQPSTAVNFDVPKGAVD
                     CHTHIHGDPAKFPFFSGRVYTPELASPEEMAALHRALHIQRVVIVTPSVYGTDNSPTL
                     FGMKARGKDARGIAVIDDKTTEAELDAMGKAGIRGVRLNLATGGINDPAVARPRFTAA
                     AQRIKARGWHLQIYTNPQMITAVKDLVMSSPVPVVFDHFGGAQAAAGVGQPGFSDLVE
                     AVRAGKAYVKISGAYRSSKLGPDYADVAPMAKALIAANPDRIVWGTDWPH"
     gene            959..1609
                     /gene="can"
                     /locus_tag="VF309_07445"
     CDS             959..1609
                     /gene="can"
                     /locus_tag="VF309_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX7466457.1"
                     /translation="MSSLSHLFESNRAWSERIRASDPEFFPRLSRQQRPRYLWIGCAD
                     SRVPANEIVDLAPGELFVHRNLANVVAHSDLNCLSVMQFAVDVLKVEHIIVCGHYGCS
                     GVSAALQKDRVGLADNWLRYVQDVHSKHDARVAALPGVPERIDRLCELNVIEQVANVC
                     QTTIVRDAWERGQELAVHGWVYGLEDGLVRDLGTVVTKPAEASGAYRAALAALQDP"
BASE COUNT          263 a          570 c          537 g          281 t
ORIGIN      
        1 atggggccag tccgttcccc acacgatgcg gtcgggattc gcggcgatga gcgccttcgc
       61 catcggcgcg acgtccgcat agtcgggacc cagtttcgac gagcggtagg cgcccgagat
      121 cttcacgtac gccttgccgg cgcgcaccgc ctcgaccagg tcggagaagc ccggctgtcc
      181 caccccggcc gcggcctgcg cgccgccgaa gtgatcgaag accaccggca cgggcgagga
      241 catcacgagg tccttcaccg cggtgatcat ctgcggattg gtgtagatct gcaggtgcca
      301 gccacgcgcc ttgatgcgct gggccgctgc ggtgaatcgc ggacgcgcca cggcgggatc
      361 gttgatgccg ccggtcgcga ggttgagcct cacgccgcgg attcccgcct tgcccatggc
      421 gtcgagctcg gcctcggtcg tcttgtcgtc gatcaccgcg atgccacgag cgtccttgcc
      481 gcgcgccttc atcccgaaga gcgtcgggga attgtcggtg ccatagacgc tcggcgtcac
      541 gatcaccaca cgctggatgt gtagcgcgcg atgaagcgcc gccatttcct cgggcgaagc
      601 gagctcgggc gtatagacgc gcccgctgaa gaagggaaac ttcgccggat cgccgtggat
      661 gtgcgtgtgg caatcgaccg cgcccttggg cacgtcgaaa ttcaccgcgg tcgaaggctg
      721 cgcggccttc gcccgcgcca ggcggctccc gcccaacagt gccgcggccg cggctcccag
      781 caagacatcg cgacgtgtcg tcatggcttc ctcctcatgg tgttgtttgc cggcatggta
      841 ccccgatcgc atcgcctgcg ctgcggcccg gatttcgcgg cgacaggaca gatccctcca
      901 ttcttggaat aatcccgcca tggcccagaa ccagaagacg cagtactagg atcgcgcgat
      961 gtcctccctc tcccatctgt tcgagagcaa ccgcgcgtgg tccgagcgaa tccgcgcgag
     1021 cgatcccgaa ttctttccgc ggctctcgcg ccagcaacgc ccgcgctatc tgtggatcgg
     1081 atgcgccgac agccgcgtgc cggccaacga gatcgtcgat ctcgcgcccg gcgagctctt
     1141 cgtccaccgc aacctcgcga acgtcgtcgc ccacagcgac ctcaactgcc tttcggtgat
     1201 gcagttcgcc gtggacgtgc tgaaggtcga gcacatcatc gtgtgcgggc actatggatg
     1261 cagcggcgtc tcggcggcat tgcagaagga ccgcgtgggg ctcgcggaca actggctgcg
     1321 ctacgtgcag gacgtgcatt cgaagcacga cgcgcgcgtg gccgcgcttc ccggcgtccc
     1381 ggagcgcatc gatcggctct gcgagctcaa cgtgatcgag caggtcgcca acgtttgcca
     1441 gacgaccatc gttcgcgatg cgtgggagcg cggccaggag ctcgcggtgc acggctgggt
     1501 ctacggcctg gaggacgggc tggtgcgcga cctcggcacc gtggtcacga agcccgcgga
     1561 agcctcgggc gcctaccgcg ccgcgctcgc cgcgctccag gacccatgat gatgaagaac
     1621 gactatccct gcgaccactt cctcgcgatt c
//
LOCUS       DASVII010000571         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_421987,
            whole genome shotgun sequence.
ACCESSION   DASVII010000571 DASVII010000000
VERSION     DASVII010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421987"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /gene="guaA"
                     /locus_tag="VF309_07450"
     CDS             complement(<1..1398)
                     /gene="guaA"
                     /locus_tag="VF309_07450"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7466458.1"
                     /translation="MHDRILILDFGAQYTQLIARRVREAKVYCEIHPCDVGDAFVREF
                     APKAVILSGSHASAYEDSTLKAPRAAFELGVPVLGICYGMQTMAAQLGGAVEPGKVRE
                     FGYAEVRARGHSRLLEGIQDRVNEKGEAFLDVWMSHGDKVTKLPPGFKVIAENPACPL
                     AGMADESRRFYAVQFHPEVTHTKQGKAIYTRFVHEICGCGAQWNMPQFVPQSVARIRE
                     QVGRDDVILGLSGGVDSAVAAALIHKAIGDQLTCIFVDTGLLRLNEGDQVMETFAKHL
                     GVKVDRVNASAEFYGALAGVSDPEEKRKVIGRVFVEVFQREAKKLPSARWLAQGTIYP
                     DVIESAGTKTKKAVTIKSHHNVGGLPESLHLKLLEPLRELFKDEVRLLGIELGLPREM
                     VFRHPFPGPGLGVRILGEVQAEYAQLLRRADAIFIEELRAAKNEDGVTWYDQVAQAFA
                     VFLPVRSVGVMGDGRT"
     gene            complement(1436..>1864)
                     /locus_tag="VF309_07455"
     CDS             complement(1436..>1864)
                     /locus_tag="VF309_07455"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7466459.1"
                     /translation="IAKALGAGANVVMLGSLFAGTEEAPGEIELYQGRSYKSYRGMGS
                     LGAMQKGSADRYFQDSEMNVDKLVPEGVEGRVPFKGSALSMIEQLLGGVRASMGYTGC
                     GTIDELRTKSRFVEITSAGMRESHVHDVQIVKEAPNYRVE"
BASE COUNT          299 a          667 c          584 g          314 t
ORIGIN      
        1 ggtgcgcccg tcgcccatca cgcccaccga gcgcaccggg agaaagaccg cgaacgcctg
       61 ggcaacctgg tcgtaccacg tcacgccgtc ctcgttcttc gccgcgcgca gctcctcgat
      121 gaagatcgca tcggcgcgcc gcagcagctg cgcatactcg gcctgcactt cgccgaggat
      181 gcgcacgccc aggccgggcc ccgggaaggg atggcgaaac accatctcgc gcggcaaccc
      241 gagctcgatg ccgaggagcc gcacttcgtc cttgaagagc tcgcgcagcg gctcgaggag
      301 cttcaggtgc aggctctcgg gaaggccgcc cacgttgtgg tgcgacttga tggtcaccgc
      361 cttcttggtc ttcgtccccg cggactcgat cacgtcgggg tagatcgttc cctgcgcgag
      421 ccatcgcgcc gacggaagct tcttcgcctc gcgctggaac acctccacga aaacgcgccc
      481 gatgaccttg cgcttctcct ccggatccga gacgcccgcg agcgccccgt agaattcggc
      541 cgacgcgttg acgcgatcga ccttcacgcc gaggtgcttc gcgaaggtct ccatcacctg
      601 gtcgccctcg ttcaggcgca gcagcccggt atccacgaag atgcaggtga gctggtcgcc
      661 gatcgccttg tggatgagcg ccgcggcgac cgccgaatcg acgccgcccg ataggcccag
      721 gatcacgtcg tcgcggccca cctgctcgcg aatgcgcgcg accgactgcg gcacgaactg
      781 cggcatgttc cactgcgccc cgcacccgca gatctcgtga acgaagcgcg tgtagatcgc
      841 cttgccctgc ttcgtgtggg tgacctcggg atggaactgc acggcgtaga agcgccgcga
      901 ctcgtcggcc atgccggcga gcgggcacgc gggattctcc gcgatgacct tgaagccggg
      961 cggaagcttc gtcaccttgt cgccgtggct catccacacg tcgaggaacg cctcaccctt
     1021 ctcgttcacg cgatcctgga tgccctcgag gaggcgcgag tgcccccgcg cgcgcacttc
     1081 cgcgtagccg aactcgcgca ccttgccggg ctccaccgcg ccgccgagct gcgcggccat
     1141 ggtctgcatg ccgtagcaga tgccgagcac cggcacgccg agctcgaacg cggccctggg
     1201 cgccttcagc gtcgaatcct cgtaggccga ggcatggctg ccggagagaa tgacggcctt
     1261 cggcgcgaat tcgcgtacga aggcgtcgcc gacgtcacag ggatggatct cgcagtagac
     1321 cttggcttcg cgcacccggc gggcgatcaa ctgcgtgtat tgcgcgccga aatcgaggat
     1381 gagaattctg tcgtgcactg tcctggaagg aggcgccggc gcgggcgccg tcgacctatt
     1441 cgacgcggta attcggggcc tccttgacga tctgcacgtc gtgcacgtgc gattcgcgca
     1501 tccctgccga ggtgatttcg acgaagcgcg acttggtgcg cagctcgtcg atggtcccgc
     1561 agcccgtata acccatgctc gcgcgcacgc cgcccagcag ttgctcgatc atcgacagcg
     1621 cgctgccctt gaacgggacg cggccctcca cgccttccgg gaccagcttg tccacgttca
     1681 tctccgaatc ctggaaatag cggtcggcgg atcccttctg catcgcgccc agcgaaccca
     1741 tgccccggta cgacttgtag gagcgtccct ggtagagctc gatctccccg ggcgcttcct
     1801 cggtgcccgc gaatagcgag cccagcatga ccacgttcgc gcccgcgccc agcgccttcg
     1861 cgat
//
LOCUS       DASVII010000572         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_206026,
            whole genome shotgun sequence.
ACCESSION   DASVII010000572 DASVII010000000
VERSION     DASVII010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206026"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VF309_07460"
     CDS             <1..1418
                     /locus_tag="VF309_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466460.1"
                     /translation="TVAEYPLYVTVIAPGELVLERWRVTSWFVGASTLILDLVIGTLT
                     LWIYRLRRRERAIVEKLRASKIAADAGNEAKSGFLAMMSHEIRTPMNGVLGMLEILSL
                     TKLDAEQRASLSVMRDSGDSLLRIIDDILDFTKIEAGKLELQPEPASVAKIVDRLCNI
                     YSGSASAKGLLLRAQVDPRISPILSFDPLRLQQILNNFVGNAIKFTSQGEISIRAEFA
                     GTRDGNEVVRFIVRDAGIGVSSEEMSKLFEPFTQAAAGSARRHGGTGLGLSICRKLAA
                     MMGGNVEMHSSPGEGTTMILMVALPIPDPSTIALFAPSASMLSAQAIAERRSPPAAGQ
                     AEDEKTLILLVDDHPVNRMVMARQVSMLGYAAETADDGFAALDLWSTGRFGLVITDVN
                     MPEMDGYELARRIREVEAGKGLPRTPIIACTANAMGGESQKCLAAGMDDYVAKPTQIP
                     QLAQKLDRWLPLAPKVLEAAL"
     gene            complement(1442..>2412)
                     /locus_tag="VF309_07465"
     CDS             complement(1442..>2412)
                     /locus_tag="VF309_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7466461.1"
                     /translation="ARVIADVFPESEVALFEGGLATSQMLLDLPFDHIFFTGSPTVGK
                     IVMVAAARHLTSITLELGGKSPAIVGETADIKRAAETLMWGKFLNSGQTCIAPDYVHV
                     HESVKDAFIEECRKVLRSRYGETDAKQKLSADLTRIVNERHAQRIAALLDDATSRGAV
                     VRSGGEVDLAQRFIAPTLLDQVPQDARILEEEIFGPLLPILGFSQLDTVIAYINARPK
                     PLALYIWSRTPEEIDRVLKRTSSGGACVNHCVIQFAHGGLPFGGVNNSGIGSAHGHFG
                     FKAFSHERAVLRSSPLMLVKLFFPPFTESRRRVIRRVVDMLRLPML"
BASE COUNT          446 a          771 c          793 g          402 t
ORIGIN      
        1 acaccgtcgc cgaatatccg ctctacgtca cggtcatcgc gccgggagag ctagtgctcg
       61 agcgctggcg ggtcacttcg tggttcgttg gagcctcgac cctcattctc gacctggtga
      121 tcggcaccct gacgttgtgg atctaccggc tgcggcggcg cgagcgggcg atcgtcgaga
      181 agttgcgcgc atcgaagatc gcggccgacg ccggcaacga ggcgaagtcg ggcttcctcg
      241 ccatgatgag ccacgagatc cgcacgccga tgaacggcgt cctcggcatg ctcgagatcc
      301 tctcgctcac gaagctcgac gcggagcaac gcgcttcgct ctccgtgatg cgcgattcag
      361 gcgattccct cctgcgcatc atcgacgaca tcctggactt caccaagatc gaggcgggca
      421 agctcgaatt gcagccggag ccggcctcgg tcgcgaagat cgtcgaccgg ctgtgcaaca
      481 tctattcggg cagcgcgagc gccaaggggc tgctgcttcg agcccaggtc gatccgcgca
      541 tcagcccgat actttcgttc gatccgttgc ggctgcagca gatcctcaac aacttcgtgg
      601 gcaacgcgat caagttcacg agccaggggg agatctcgat tcgcgcggag ttcgccggga
      661 cgcgcgatgg gaacgaagtc gtgcgcttca tcgtgaggga cgccggcatc ggcgtttcct
      721 cggaagagat gtcgaagctc ttcgagccgt tcacgcaggc cgcggcgggt tctgcccggc
      781 gccacggagg aacgggcctg gggctctcga tctgccgcaa gctcgccgcg atgatgggcg
      841 gaaacgtgga gatgcacagc agcccgggcg agggaaccac catgatcctc atggtcgcgc
      901 tgccgattcc cgatccgtcg acgatcgccc tcttcgcgcc cagcgcatcg atgctcagcg
      961 cgcaggccat cgccgagcgt cgatcgccgc ccgcggccgg gcaggccgag gatgagaaaa
     1021 cgttgatcct gctggtcgac gatcacccgg tgaaccgcat ggtgatggcg aggcaggtga
     1081 gcatgctcgg gtacgccgcc gagaccgccg acgacggctt cgccgcgctc gacctgtgga
     1141 gcaccgggcg attcgggctg gtcatcacgg acgtgaacat gccggagatg gatggctacg
     1201 agctcgcgcg ccgcattcgc gaagtcgagg ccggcaaggg actgccgcgc acgccgatca
     1261 tcgcctgcac cgcgaatgcg atgggtggcg aatcccagaa gtgcctcgcc gcggggatgg
     1321 acgactacgt cgccaagccg acgcagattc cgcagctcgc gcagaagctg gaccgctggc
     1381 tgccgctcgc accgaaggtc ctcgaggcgg ccctctgaat attccagccg accccgctgt
     1441 ttcagagcat cggcagccgc agcatgtcga ccactcgacg aatcacgcgt cggcgggact
     1501 ccgtgaaagg cgggaagaac agcttcacca gcatcagggg agatgatcgc agcactgcgc
     1561 gctcatgcga gaacgccttg aagccaaaat ggccgtgcgc gctgccgatg cccgaattgt
     1621 tcacgccgcc aaaaggcaag ccgccatgcg cgaactggat cacgcagtga ttgacgcagg
     1681 cgccgccgga gctggtgcgc ttgagcactc ggtcgatctc ttccggggtg cggctccaga
     1741 tgtacaacgc cagcggcttc gggcgcgcat tgatataggc aatgaccgta tccagctgcg
     1801 agaagccgag aatcggcagc aacggaccga aaatctcctc ttccaggatg cgcgcgtcct
     1861 gcggcacctg gtcgagcagc gtgggtgcga tgaagcgctg agcgagatcg acctcgccgc
     1921 ccgatcgcac gaccgcgccg cgcgaagtgg cgtcgtccag cagcgcggcg atgcgctgcg
     1981 cgtggcgttc gttgacgatg cgcgtcaaat ccgcactgag cttctgcttg gcgtcggttt
     2041 caccgtaacg cgagcgcagc accttgcggc attcttcgat gaaggcgtcc ttcacggatt
     2101 cgtgcacatg cacgtaatcc ggagcgatgc aggtctggcc ggaattgagg aacttccccc
     2161 acatgagggt ttcggcagcc cgcttgatgt cggccgtctc gccgacgatg gccggcgact
     2221 ttcccccgag ctccagcgtg atgctggtca agtgcctggc cgcggcgacc atcacgatct
     2281 tgccaacggt gggcgatccg gtgaagaaga tatggtcgaa cggcaggtcg agcagcattt
     2341 gcgacgtggc gagcccgccc tcgaagagcg cgacctcgga ttcaggaaaa acgtcggcga
     2401 tgacgcgcgc ca
//
LOCUS       DASVII010000573         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_673989,
            whole genome shotgun sequence.
ACCESSION   DASVII010000573 DASVII010000000
VERSION     DASVII010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673989"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VF309_07470"
     CDS             complement(<1..599)
                     /locus_tag="VF309_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7466462.1"
                     /translation="MKLARTLAFPLALAIPALAAAQVTDIAAVSRLQGEARAKALVEG
                     ARREGEVMVYHSSQTEDLKPVFDAFTAKYGVKVRDWRSSSENVVQRVIAETRAGKRDV
                     DFIENNAPEMEALHREKMLLRMDSPYFADLRPGTLPQHREWATSTMDVFVAAYNTEKV
                     KREELPKTYQDLLDPRWKGRLGIEAEDQAWFGTLLGGMG"
     gene            complement(628..1938)
                     /locus_tag="VF309_07475"
     CDS             complement(628..1938)
                     /locus_tag="VF309_07475"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7466463.1"
                     /translation="MLTTQENDLLCRVEGAAPMGQLMRRHWIPVCLAEEVAEAGGTPV
                     HAKILGEDLVVFRDSDGNVGVLDEHCPHRRASLVMGRNEEGGLRCLYHGWKMDVKGNV
                     LEMASEPASSGLCAKVKHTAYPAQDWGGFVWAYMGPAESMPAFEPPAFAPTPQTRVSI
                     VKVQVACNWAQILEGAIDSAHSSSLHSTDMPPARVDGAKATETVWPRPSTDKAPRLQV
                     QQTPFGFRYAAIRRPIVNSATHDYIRITLFQAPFSVHIPPNNLYNLSILHIPQDDCNT
                     YFYFVAWSDAGAGIDQDSWRKFCGAQVGVDLDWKYRKYRNRDNNYLQDRKAMKLGDFT
                     GIRGIPMQDMAMWETMGGIADRSRERLGASDLAIVEFRRIMVEAARQFAAGKPAIGTT
                     EPRVAAAKLRSFEGIVAKSTDWRTLDGAPAPAQASAATQEHAAD"
     gene            2041..3000
                     /locus_tag="VF309_07480"
     CDS             2041..3000
                     /locus_tag="VF309_07480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX7466464.1"
                     /translation="MTSLPAPLTLRVARAEKVAADIHSFELRSPSGGELPPFTAGAHV
                     TVKSRNGSLRKYSLCNDPAERDRYVIAVKRDPAGRGGSVDLVDHTRVGDGLEVCAPRN
                     AFELAASAPSFILIAGGIGIAPILSMARSLNAEGRRYTLYYLARSPAHAAFRDELQRG
                     EFAGKVVIHHDGGDPARSFDLWPILEKPSAAHVYCCGPRPLLGEVRDMTGHWPAAAIH
                     FESFIDAGAAAKPGDRPFAVVLAKSGDRLEVPVGLSILEVMRSRGHDAPSSCESGTCG
                     TCRTRLISGEADHRDLVLMEDEKATQIMICVSRARSPEIVIDR"
BASE COUNT          447 a         1092 c         1034 g          516 t
ORIGIN      
        1 tcgcccatcc ctcccagcag ggtgccgaac cacgcctggt cctccgcttc gatgccgagc
       61 cggcctttcc agcgcgggtc gaggagatcc tggtaggtct tcggcagctc ctcgcgcttc
      121 accttctcgg tgttgtacgc cgccacgaac acgtccatgg tggatgtcgc ccactcgcgg
      181 tgctgcggca acgtgccggg ccgcaggtcc gcgaagtagg gagaatccat ccgcaggagc
      241 atcttctcgc gatgcaacgc ctccatctcg ggcgcgttgt tctcgatgaa atcgacgtcg
      301 cgcttgccgg cgcgggtctc ggcgatgacg cgctggacga cgttctccga gctcgagcgc
      361 caatcgcgga ccttcacgcc gtacttggcg gtgaaggcgt cgaagaccgg cttcaggtcc
      421 tcggtctggc tcgagtgata gaccatcacc tcaccctcgc ggcgcgcgcc ctcgacgagc
      481 gctttggcgc gcgcctcgcc ctgcaggcgc gagacggcgg cgatgtcggt cacctgcgcc
      541 gcggcgagcg ccgggatggc gagcgcgagc gggaacgcca gggtgcgcgc gagcttcatg
      601 cccacttctc cttggaaggc cgcgaattca atcggcagca tgctcctgcg tcgccgcgga
      661 cgcctgggcg ggggcgggcg cgccgtcgag cgtgcgccaa tcggtggact tcgcgacgat
      721 gccctcgaag gagcgcagct tcgccgcggc gacgcgcggc tcggtggtgc cgatggcggg
      781 cttgcccgcc gcgaactgcc tcgccgcctc caccatgatg cggcggaatt ccacgatcgc
      841 gaggtcgctc gcgccgaggc gctcgcggct gcggtcggcg atcccgccca tcgtctccca
      901 catcgccatg tcctgcatcg gaatgccgcg gatgcccgtg aagtcgccga gcttcatcgc
      961 cttgcgatcc tgcaggtagt tgttgtcgcg attgcgatat ttccggtact tccagtcgag
     1021 gtcgacgccg acctgcgcgc cgcagaactt gcgccacgaa tcctggtcga tgcccgcgcc
     1081 ggcgtcgctc cacgcgacga agtagaaata cgtgttgcag tcgtcctggg ggatgtggag
     1141 gatcgaaagg ttgtacaggt tgttcggcgg gatgtgcacc gagaacggcg cctggaagag
     1201 cgtgatgcgg atgtagtcgt gggtggccga gttcacgatc ggccggcgaa tcgccgcgta
     1261 gcggaatccg aacggcgtct gctgcacctg caggcgcggc gccttgtccg tcgaggggcg
     1321 cggccacacc gtctcggtcg ccttcgcgcc gtcgacgcgc gcgggaggca tgtccgtcga
     1381 atgcaggctc gagctgtggg ccgagtcgat cgcgccctcg aggatctgcg cccagttgca
     1441 cgccacctgc accttcacga tggaaacgcg cgtctgcggc gtcggcgcga aggcaggcgg
     1501 ctcgaaggcg ggcatcgatt ccgccgggcc catgtacgcc cacacgaatc cgccccagtc
     1561 ctgcgccgga taggcggtgt gcttcacctt ggcgcacagg ccgctcgagg cgggctcgga
     1621 agccatctcg aggacattgc ccttcacgtc catcttccag ccgtgataca ggcagcgcag
     1681 cccgccttcc tcgttgcgtc ccatcacgag ggaggcgcgc cggtgcgggc aatgctcgtc
     1741 cagcacgccg acgttgccgt cgctgtcgcg gaacaccacc aaatcctcgc ccaggatctt
     1801 cgcgtgcacc ggggtgccgc cggcctcggc gacctcctcg gcgaggcaca cgggaatcca
     1861 gtggcggcgc atgagctggc ccatgggcgc ggcgccttcg acgcggcaca agaggtcgtt
     1921 ttcctgggtc gtcagcatgg gacctccgat cgatatttgc ccgcgccgcg cgcgcggtgt
     1981 aagttagacc tctaaggtta tcaaacctac gggctttcgg cacctgcgtc cacgcgagcc
     2041 atgacctccc tccccgcgcc gctcaccctg cgggtcgccc gcgccgaaaa ggtcgcggcg
     2101 gacatccact cgttcgagct gcgcagccct tcgggcggcg agctcccgcc cttcacggcc
     2161 ggcgcgcacg tcacggtgaa atcccgcaac gggagccttc gcaagtactc gctgtgcaac
     2221 gatccggccg agcgcgaccg ctacgtgatc gcggtgaagc gcgatcccgc ggggcgcggc
     2281 ggctccgtgg acctggtgga ccacacgcgc gtcggcgacg ggctcgaagt ctgcgcgccg
     2341 cgcaacgcct tcgagctcgc cgcaagcgcg ccttccttca tcctcatcgc gggaggcatc
     2401 ggcatcgccc cgatcctgtc gatggcgcgc agcctcaacg ccgagggccg tcgctacacg
     2461 ctctactatc tcgcgcgctc acccgcgcac gccgcgttcc gcgacgagct gcaacgcggg
     2521 gaattcgccg ggaaggtggt catccatcac gacggcggcg atcccgcgcg ctcgttcgat
     2581 ctctggccga tcctcgagaa gccttccgcc gcccacgtgt attgctgcgg cccgcgcccg
     2641 ctcttgggcg aggtgcgcga catgacgggc cactggccgg cggccgccat ccacttcgaa
     2701 agcttcatcg acgcgggcgc cgccgcgaag cccggcgaca ggcccttcgc cgtggtgctc
     2761 gcgaaatcgg gcgatcgcct cgaggtgccc gtgggccttt ccattctcga ggtcatgcga
     2821 agccgcggcc acgatgcgcc gagctcgtgc gaaagcggca cttgcggcac ctgccgcacg
     2881 cgcctcatct ccggcgaggc cgaccatcgc gacctcgtcc tcatggaaga cgagaaggcg
     2941 acgcagatca tgatctgcgt gtcgcgggcg cggtcgcccg agatcgtgat cgaccgctga
     3001 tggcgcgccg cgtgcgtctc ggcgtcgcgg ggctcggccg cgcgttcacg ctgatgctgc
     3061 cgacgctttc cgcccatccg ggcgtggag
//
LOCUS       DASVII010000574         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_134762,
            whole genome shotgun sequence.
ACCESSION   DASVII010000574 DASVII010000000
VERSION     DASVII010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134762"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF309_07485"
     CDS             complement(<1..478)
                     /locus_tag="VF309_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7466465.1"
                     /translation="MSANQILVVDDEVGIRELLFEILRDEGYGVRLAENAQTARSARK
                     EMRPDLVLLDIWMPDTDGITLLKEWAGSGLLTMPVVMMSGHGTIDSAVEATRIGAFDF
                     LEKPISLPKLLATVGKALAGGRVLPKTGLALVNLGRARVIQELRQRLEQVGRLRTPV"
     gene            complement(491..>1591)
                     /locus_tag="VF309_07490"
     CDS             complement(491..>1591)
                     /locus_tag="VF309_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466466.1"
                     /translation="TMTRQIADATDAMERNQQQLENSKTYLESILSNLTSGVLTFDER
                     LYVKTMNAAANAILAVPGGAFHGIKLSEWPRHVQSVAPFAEIALRQFASSGMRQWEEQ
                     MEYRRADGQRTLLIRGTRLGARGEEGYVVVFDDITHLIQAQRDAAWGEVARRLAHEIK
                     NPLTPIQLSAERLQLKLKDKLPENEAEILKRATSTIVTHVAALKGMVDDFTQYAHASR
                     MNAKAVNLNDLVREVLVLYEAMGVAIEPRLAESLPSIYADPAMLRQVLHNLFQNAIDA
                     LTGVENPRILVNTSMGTGGVLLTVRDNGTGIAEGVMGRIFEPYVTTKPKGTGLGLAIV
                     KKIVDEHHGRILVENVKPHGATVSIVLPSKAA"
BASE COUNT          237 a          522 c          541 g          291 t
ORIGIN      
        1 gcaccggggt gcgcagccgc ccgacctgct cgaggcgttg acgcagctcc tggatcacgc
       61 gcgcgcgtcc caggttcacc aacgcgaggc cggtcttggg cagcacgcgc ccgccggcaa
      121 gcgccttgcc caccgtggcg aggagcttgg gcaggctgat cggcttctcg aggaaatcga
      181 aggcgccgat gcgtgtcgcc tctaccgccg aatcgatggt gccgtggccc gacatcatca
      241 ccaccggcat ggtgagcagc ccgcttcccg cccactcttt gaggagcgtg atgccgtcgg
      301 tatcgggcat ccagatgtcg agcagcacga ggtcggggcg catctccttc ctcgcggagc
      361 gcgcggtctg cgcgttctcg gcgaggcgca ccccatagcc ctcgtcgcgc aggatctcga
      421 agagcaactc acggatgccg acttcgtcgt cgacgacgag gatctggttc gcggacatgc
      481 gtgggcgccg ttaggcggcc ttggaaggca ggacgatgct cacggtggcg ccgtgcggct
      541 tgacgttctc caccaggatc ctgccgtgat gttcatcgac gatcttcttc acgatcgcga
      601 gcccgagccc ggttcccttg ggcttggtgg tcacgtacgg ctcgaagatc cggcccatga
      661 cgccctcggc gatgccggtg ccgttgtcgc gaaccgtgag cagcactccg cccgtgccca
      721 tgctcgtgtt caccaggatg cgcgggttct ccacgccggt gagggcgtcg atcgcgttct
      781 ggaagaggtt gtgcaggacc tggcgcagca tcgccgggtc ggcgtagatc gacggcaggc
      841 tctcggcgag gcgcggctcg atcgccacac ccatcgcctc gtagaggacg agcacctcgc
      901 gcaccaagtc gttcaggttc accgccttgg cgttcatgcg cgaggcgtgg gcgtactggg
      961 tgaagtcgtc caccatgccc ttcaacgccg ccacatgggt cacgatcgtg ctcgtggcgc
     1021 gcttcaggat ctcggcctcg ttctcgggca gcttgtcctt cagcttcagc tgcaggcgct
     1081 ccgcggagag ctggatcggg gtgagcggat tcttgatctc gtgcgcgagg cggcgcgcga
     1141 cctcgcccca ggcggcgtcg cgctgcgcct ggatcaaatg agtgatgtcg tcgaacacga
     1201 ccacgtagcc ttcctcgcca cgcgcgccaa ggcgcgtacc gcgaatgagg agggtgcgct
     1261 gcccgtccgc gcggcggtat tccatctgct cttcccactg gcgcatgccc gaggaggcga
     1321 actggcgcag cgcgatttcc gcgaacgggg cgaccgactg cacgtggcgc ggccactccg
     1381 acagcttgat gccgtggaag gcgccgccgg gcacggcgag gatggcattg gccgcggcgt
     1441 tcatggtctt cacgtacagg cgctcgtcga aagtgagaac ccccgacgtc agattggaga
     1501 ggatgctctc gagataggtc ttggagttct ccagctgctg ctggttgcgc tccatcgcgt
     1561 cggtcgcgtc ggcgatctgg cgcgtcatgg t
//
LOCUS       DASVII010000575         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_206284,
            whole genome shotgun sequence.
ACCESSION   DASVII010000575 DASVII010000000
VERSION     DASVII010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206284"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VF309_07495"
     CDS             complement(<1..727)
                     /locus_tag="VF309_07495"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7466467.1"
                     /translation="MSSATFCAVFAAFALAALAWRMWLGVRQMRHVASHRDAVPADFA
                     AYIPIEAHRKAADYTLDKQRVALAEALVIDGALLFFLTLGGGVAMIDAWSHAHLGDGY
                     LRDLGTVFGVMIVSGVLALPFDAWRTFVIESRHGFNRTTPAVFVLDLLKGALLAVALG
                     APLLLGVFWLVDAAGPYWWLYAWIGWIAFTLALVMVYPRWIAPLFNRFTPLEEGDLRR
                     RIEALIERCGFHAKGLFVMDGSRR"
     gene            793..1356
                     /gene="orn"
                     /locus_tag="VF309_07500"
     CDS             793..1356
                     /gene="orn"
                     /locus_tag="VF309_07500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX7466468.1"
                     /translation="MAHDKTPLVWIDMEMSGLVPERDRILEVAMVVTDSELNTIAEAP
                     VYVVHQSDEVLEGMDSWNKSTHGKSGLIDKVRASTFTEAEVEMRLVEFLKPIVGERLA
                     PLCGNTVHQDRRFMARYMPAFDAYLHYRIIDVSTLKELAKRWHPEVLQGVAKEGRHEA
                     LADVYESVEELRHYRRTFLRAEPEAAS"
BASE COUNT          275 a          496 c          525 g          206 t
ORIGIN      
        1 agcggcgcga gccgtccatc acgaagaggc ccttcgcgtg gaagccacag cgctcgatga
       61 gcgcctcgat gcgccgccgc aggtcgcctt cctcgagcgg cgtgaagcgg ttgaagagcg
      121 gcgcgatcca gcgcggatag accatcacca gcgcgagggt gaacgcgatc cacccgatcc
      181 aggcgtagag ccaccagtag ggcccggccg cgtctaccag ccagaagacg cccagcagca
      241 ggggcgcgcc cagcgccacg gcgagcagcg ctcccttcag gagatcgagg acgaagaccg
      301 ccggcgtcgt gcggttgaag ccgtgacgcg actcgatcac gaacgtgcgc cacgcatcga
      361 agggaagcgc gagcacgccc gagacgatca tcacgccgaa gacggtgccc aggtcgcgca
      421 ggtagccgtc gccgagatgc gcgtgcgacc acgcgtcgat catcgccacg ccgccgccca
      481 gcgtgaggaa gaagagcagc gcgccatcga tcacgagcgc ctcggcgagc gcgacgcgct
      541 gcttgtcgag cgtgtagtcg gcggccttgc ggtgggcctc gatcgggatg taggcggcga
      601 agtccgcggg aactgcgtcg cggtggcttg ccacgtggcg catctggcgc acgccgagcc
      661 acatgcgcca ggcgagggcg gcgagggcga aggcggcgaa gacggcgcag aaggtggcgg
      721 agctcacgat tggtcaagac cgggttggcg tgacacaatc gacacccgat tttcgcggac
      781 gcgaaggctc aagtggcgca cgacaagacc ccgttggttt ggatagacat ggagatgtcg
      841 ggcctcgttc ccgagcgcga ccgcattctc gaggtcgcca tggtggtgac cgactcggag
      901 ctgaacacca tcgcggaggc cccggtgtac gtggtccacc agtccgacga ggttctcgag
      961 gggatggact cctggaacaa gtcgacgcac ggcaagtccg gcctcatcga caaggtgcgc
     1021 gcctcgacat tcaccgaggc cgaggtcgag atgcgcctcg tcgaattcct gaagcccatc
     1081 gtgggggagc gcctggcgcc gctttgcggc aacacggtgc accaggaccg gcgcttcatg
     1141 gcgcgctaca tgcccgcctt cgacgcctat ctccactatc gcatcatcga cgtctcgacg
     1201 ctgaaggagc tcgccaagcg ctggcatccg gaagtgctgc agggagtcgc gaaggagggc
     1261 cggcacgagg cgctcgcgga cgtctacgag tcggtcgagg agctgcggca ctaccgccgc
     1321 accttcctgc gcgcggagcc ggaagcggcg agctgattca ggcctcgcgc gtccaatcgc
     1381 gggcgcgccc gacggcgcgc ttccattcgc cgagcttcgc gagcgccgtc tcgcgcgcga
     1441 gccgcggctc gaacacgcga tcgcgcgacc agagcgaggc gatctcgttt tcatccttcc
     1501 ag
//
LOCUS       DASVII010000576         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_242933,
            whole genome shotgun sequence.
ACCESSION   DASVII010000576 DASVII010000000
VERSION     DASVII010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242933"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF309_07505"
     CDS             <1..209
                     /locus_tag="VF309_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466469.1"
                     /translation="DKRFPESADIPGVYMLAATILLEKRGDYAMAQKIFLHLAKKFPQ
                     HPLAAEATRLAQVAEKMAVGAPAA"
     gene            complement(214..>1631)
                     /locus_tag="VF309_07510"
     CDS             complement(214..>1631)
                     /locus_tag="VF309_07510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7466470.1"
                     /translation="RRPPPVTVALILVNCLVFFFYQTGDSAREAKALEAYSSSSLPRL
                     ELPAFKERLRETNPKLAPFVDRADPAALVGRMESDEVFMRGLRAGRVVTPQNPDYAKW
                     KEDRARFEALLAKVSYRRFGFTPADPRPVTWLASMFLHGGLMHLLGNMVFLFIVGVAV
                     ESALGGFWYLLLYLAGGLAGDAMLYVVNPSSILPTVGASGAISGLMGLFTVVFGLRKV
                     NFFYWVIVFFGFKQMRGLVVLPIWIGWEILQYVTNKGSGVAYMAHAGGLMGGALLGLA
                     VTKRFAGARVEQFHEEREQEAFDKAEYERARALVAKLDFKAASTVFARLAQRFPQEEM
                     LLRQWHAIAKSDTGSEAYHRSVNGILALTKGGAALRTFQRQTFNEYFASAKPAARLEP
                     QVLAAIGLQFARAGELAEAERAADVLFKQAPGEPRLAILWEALAHALDKSAGEGSVAK
                     AKRYRALIGARARAARAPRPA"
BASE COUNT          280 a          564 c          553 g          234 t
ORIGIN      
        1 tcgacaagcg cttcccggag agcgccgaca ttcccggcgt ctacatgctc gccgcgacga
       61 tcctcctcga gaagcgcggc gattacgcga tggcgcagaa gatcttcctg cacctggcga
      121 agaagtttcc gcagcacccg ctcgccgccg aagcgacgcg gctcgcgcag gttgcggaaa
      181 agatggccgt gggagcgccc gcggcctagc cgctcacgcg ggacgcgggg cgcgggcggc
      241 cctcgcgcgg gcgccgatga gcgcgcggta gcgcttcgct ttcgcaacgc ttccttcgcc
      301 cgcggacttg tcgagcgcat gggccagcgc ctcccacagg atcgcaagac gcggctctcc
      361 cggcgcctgc ttgaagagca cgtcggcggc gcgctccgcc tccgcaagct cgccggcgcg
      421 cgcgaactgc aggccgatcg cggccagcac ctgcggctcg aggcgcgcag caggcttcgc
      481 gctcgcgaaa tactcgttga aggtctgccg ctggaaggtt cgcagcgccg cgcccccttt
      541 cgtaagcgcg aggatcccgt tcaccgagcg gtgatacgcc tcgctgccgg tgtccgactt
      601 ggcgatcgca tgccattgcc tgagaagcat ctcctcctgg gggaagcgct gcgcgagccg
      661 cgcgaacacg gtgctcgccg ccttgaagtc gagcttcgcg acgagggcac gcgcccgctc
      721 gtactccgcc ttgtcgaacg cctcctgctc gcgttcctcg tggaactgct cgacgcgcgc
      781 gcccgcgaag cgcttcgtca ccgcgagtcc gagcaaggcc ccgcccatga ggccgcccgc
      841 gtgcgccatg taggcgacgc ccgagccctt gttggtgacg tactggagga tctcccagcc
      901 gatccagatc ggcagcacga cgaggccgcg catctgcttg aagccgaaga agacgatcac
      961 ccaatagaag aaattcacct tgcgcagccc gaagacgacg gtgaagagcc ccatcagccc
     1021 cgagatcgct cccgatgcgc ccaccgtggg caggatgctt gacggattca cgacgtagag
     1081 catcgcatcg ccggcgagcc cgcccgcgag atagagcagc aggtaccaga agccgcccag
     1141 cgcgctctcg accgccaccc ccacgatgaa gaggaacacc atgttgccga gcaggtgcat
     1201 gaggcccccg tgcaggaaca tgctggcgag ccaggtgacg gggcgcgggt cggccggcgt
     1261 gaagccgaat cgacgatagg agaccttggc gaggagcgcc tcgaagcgcg ccctgtcctc
     1321 tttccatttc gcgtagtcgg ggttctgcgg cgtgacgacg cggccggcgc gaagcccgcg
     1381 catgaagact tcgtccgact ccatgcgccc gaccagggcc gcggggtcgg ctctgtcgac
     1441 gaaaggcgcg agcttggggt tcgtctcgcg cagccgctcc ttgaacgcgg gcagctcgag
     1501 gcgcggcagc gacgacgagg aataggcttc gagcgccttc gcctcgcggg cggagtcgcc
     1561 ggtctggtag aaaaagaaaa ccaggcagtt gacgaggatg agggcgacgg tgaccggcgg
     1621 cggccggcgc c
//
LOCUS       DASVII010000577         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_530124,
            whole genome shotgun sequence.
ACCESSION   DASVII010000577 DASVII010000000
VERSION     DASVII010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530124"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VF309_07515"
     CDS             <1..955
                     /locus_tag="VF309_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX7466471.1"
                     /translation="GAQQDLMLRVAQAYFDVLLAHDNVALSETQSVAISEQLAQAKRN
                     FEVGTATIVDTLDAQARYDQTVAKAISDKNDLEVKRRTLQALIGTLPESLTPLREPLQ
                     LGEPKPNDIEAWVKSSQDSSYAIANARANFEISRQEVDRQRAGHLPTLDLSGSFARAT
                     NPSTSVPGVIGATANTSAIGLILSIPIYSGGLTQARVREALANRERAAQDLENTQRTI
                     ALSVRTNFLNVTNGISQVRALEQALASTQSQLDSTILGRDVGVRTSVDVLNAQQAVFQ
                     TRRDLQQARYNFLLSNLRLKAASGTITDADIEEVNRTLARA"
     gene            complement(963..>1846)
                     /locus_tag="VF309_07520"
     CDS             complement(963..>1846)
                     /locus_tag="VF309_07520"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX7466472.1"
                     /translation="IILETEIWPRLLEEAARMGIPVVLANARLSERSARRYAWIPSLT
                     RWSFANLRGVAAQTEADALRFAAIGARDPVVLGNVKFDLAVPPQMIERGREFRARFGE
                     ARAVWVAGSTREGEEALLLDAFIEQGARDDALLVLVPRHPQRFDDVVHLAESRGLAVS
                     RRSAAEAVPADVRVVVGDSMGEMFAYYAAADVVIMGGSLLEFGSQNLIEACALGRPVI
                     VGPHTFNFAEAAASAIAAGGAVRVNDAREALAEAAAISGDAQRREAMGTKALEFVAAH
                     RGAVERLMDWIETKTAR"
BASE COUNT          299 a          698 c          604 g          245 t
ORIGIN      
        1 cggcgcgcag caggacctca tgctgcgtgt ggcccaggcc tacttcgatg tgctcctcgc
       61 gcacgacaac gtcgcgctct cggagaccca gagcgtcgcc atttccgagc agctcgcgca
      121 ggccaagcgc aacttcgagg tgggcacggc gaccattgtc gacacgctcg acgcgcaggc
      181 acgctacgac cagacggtgg ccaaggcgat ctccgacaag aacgacctcg aggtgaagcg
      241 ccgcacgctg caggcgctga tcggcacgct tccggaatcg ctcacgccgc tgcgcgagcc
      301 gctccagctc ggcgagccca agcccaacga catcgaggcg tgggtcaagt cctcgcagga
      361 ctcgagctac gccatcgcca acgcgcgcgc caacttcgag atctcccgcc aggaggtgga
      421 ccgccaacgc gcgggacacc ttccgaccct cgacctgagc ggcagcttcg cgcgcgcgac
      481 caatccgtcg acgagcgttc ccggagtcat cggcgccacc gcgaacacca gcgcgatcgg
      541 gctcatcctg tcgatcccga tctactcggg cgggctcacc caggcgcggg tgcgagaggc
      601 gctcgccaac cgcgagcgcg cggcgcagga tctcgagaac acgcagcgca ccatcgcgct
      661 gtcggtgcga accaatttcc tcaacgtgac gaacggcatt tcccaggtgc gcgccctgga
      721 gcaggcgctc gcctcgacgc agagccagct cgactcgacc atcctcggcc gcgacgtggg
      781 cgtgcgcacg agcgtggacg tgctgaacgc gcagcaggcg gtcttccaga cgcgccgcga
      841 cctgcagcag gcgcgctaca acttcctgct gagcaacctg cgcctcaagg cggcttcggg
      901 cacgatcacc gacgccgaca tcgaggaagt gaaccgcacc ctcgcgcgcg cctagaggct
      961 gcctagcgcg cggtcttcgt ctcgatccag tccatcaggc gctctaccgc gccgcggtgc
     1021 gccgcgacga actcgagcgc cttcgttccc atcgcctccc gccgctgcgc gtcgccgctg
     1081 atcgccgccg cttcggcgag cgcttcgcgc gcgtcgttca cgcgcaccgc gccgcccgcg
     1141 gcgatcgcgc tcgccgcggc ctcggcgaag ttgaacgtgt gcggccccac gatcacgggt
     1201 cggccgagcg cgcacgcctc gatcaggttc tggctgccga actcgagaag gctgccaccc
     1261 atgatcacga cgtcggccgc ggcgtagtag gcgaacatct cgcccatcga atcgcccacg
     1321 accacgcgca catccgccgg caccgcctcg gccgcgctgc gccgcgacac ggcgaggccg
     1381 cggctttcgg cgaggtgcac cacgtcgtcg aaacgctgcg gatggcgcgg cacgaggacc
     1441 agcaacgcgt cgtcgcgcgc gccctgctcg atgaatgcgt cgaggaggag cgcctcttcg
     1501 ccctcgcgcg tgcttccggc gacccacacc gcgcgcgcct cgccgaagcg ggcgcggaac
     1561 tcgcggccgc gctcgatcat ctgcggcggc accgcgaggt cgaacttcac gttgccgagg
     1621 accacgggat cgcgcgcgcc gatcgccgcg aagcgcagcg cgtcggcctc ggtctgggcc
     1681 gcgaccccgc gcaggttcgc gaaggaccag cgcgtgagcg agggaatcca tgcgtagcgg
     1741 cgcgcggagc gctccgagag ccgcgcgttc gcgagcacca ccgggattcc catgcgcgcg
     1801 gcttcctcga gcaggcgcgg ccagatctcc gtctcgagga tgatgc
//
LOCUS       DASVII010000578         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_458248,
            whole genome shotgun sequence.
ACCESSION   DASVII010000578 DASVII010000000
VERSION     DASVII010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458248"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1710
                     /gene="gspD"
                     /locus_tag="VF309_07525"
     CDS             <1..>1710
                     /gene="gspD"
                     /locus_tag="VF309_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX7466473.1"
                     /translation="GYAAVEDGGFVKIVPEAEAKTSAGPVGELAARTPGDRIVTQVFM
                     LQNESAVQLVPVLRPLVTANNFIAAYPNNNAILITDYAENVRRIQRIIQSIDIPASGE
                     VQVIRLQNASAIDISQILQRLIPETVPSPTAPGTPPKVAIAVDPRTNSLLVRAEHAGL
                     MARVKALAVSLDTPGSGNGNIYVVFLRNADATRIAETLRGLLSGSGESSRTTTTSSTP
                     AGGMAQQTTATSTTGGPGPAAPPSSVIQAYPPTNSIIITAPEPMYRSLRTVIETLDQR
                     RAQVYVEALIVEVSTGRAAEFGVQWNGATNVNGGSTLFGGTNFSTTVGSNILGIGANP
                     TTIGTGLNVGLINGTITIPGTTTQVANLQLLARALEGDSSANVLSTPNVLTLDNEEAK
                     IIVGQNVPFITGSFTTAANAATNPFQTIERKDIGLTLRVTPQVSEGGGVKLKIFQEVS
                     SVTQDKSIVQSADIITNKRSLESTVLVDDGQIVVLGGLIQDDRSVSIDKVPLLGDIPY
                     IGALFKYQSRNHKRTNLMIFLRPVVLKDGRGAAALTADRYEYIRNLQGEIKEPWGGLL
                     PETK"
BASE COUNT          324 a          617 c          526 g          243 t
ORIGIN      
        1 ggctacgccg cggtggaaga cggcggcttc gtgaagatcg ttccggaagc cgaagcgaag
       61 acgagcgcgg gaccggtggg cgagctggcc gcgcgcacgc cgggcgatcg catcgtcacg
      121 caggtcttca tgctgcagaa cgagtccgcg gtgcagctgg tgccggtgct gcgcccgctc
      181 gtcaccgcca acaacttcat cgcggcgtat cccaacaaca acgcgatcct catcaccgac
      241 tacgcggaga acgtgcgccg catccagcgc atcatccagt cgatcgacat tccggcttcc
      301 ggcgaggtgc aggtgatccg cctgcagaac gcctcggcga tcgacatctc gcagatcctg
      361 cagcgcctca tcccggagac ggtgccctct cccacggcgc cgggtacgcc cccgaaggtc
      421 gcgatcgcgg tcgacccgcg caccaatagc ctcctggtgc gagcggagca tgccgggctc
      481 atggcgcgcg tcaaggcgct cgcggtgagc ctcgacacgc ccggctcggg caacggcaac
      541 atctacgtcg tgttcctgcg caatgccgac gccacacgca ttgcggagac attgcgcggc
      601 ctcctttcgg gcagcggcga atcgtcgcgc acgaccacca ccagcagcac ccccgcgggg
      661 ggcatggcgc agcagacaac ggcaacctcc accacgggcg gacccggccc cgccgcgccg
      721 ccgagctcgg tcatccaggc gtatccgccc accaactcga tcatcatcac ggcaccggag
      781 ccgatgtacc gcagccttcg cacggtcatc gagacgctcg accagcggcg ggcccaggtc
      841 tacgtcgagg cgctcatcgt cgaggtctcg acgggccgcg ccgcggagtt cggcgtgcag
      901 tggaacggcg cgaccaacgt gaacggcggc tcgacccttt tcggcggaac caacttcagc
      961 accaccgtgg gctccaacat cctcggcatc ggcgccaatc ccaccaccat cggcacgggc
     1021 ctgaacgtcg ggctcatcaa cggcacgatc accattcccg gcacgaccac gcaggtcgcg
     1081 aacctgcagc tcctcgcgcg cgcgctcgaa ggcgactcga gcgccaacgt cctctcgact
     1141 cccaacgtcc tcacgctcga caacgaggaa gcgaagatca tcgtcggcca gaacgtgccg
     1201 ttcatcacgg ggtcgttcac cacggccgcg aacgcggcga cgaacccgtt ccagaccatc
     1261 gaaaggaagg acatcggcct caccttgcgc gtgaccccgc aggtctcgga gggaggcggc
     1321 gtgaagctca agatcttcca ggaagtctcg agcgtcaccc aggacaagtc gatcgtgcag
     1381 tcggccgaca tcatcaccaa caagcgctcg ctcgaatcca cggtcctcgt cgacgacggc
     1441 cagatcgtgg tgctgggggg gctcatccag gacgatcgct cggtgtccat cgacaaggtg
     1501 ccgctgctgg gcgacattcc ctacatcggc gcgcttttca agtaccagtc gcgcaaccac
     1561 aagcgcacca acctcatgat cttccttcgc cccgtggtgc tgaaggacgg ccgcggggcc
     1621 gcggcgctca ccgcggatcg ctacgagtac atccgcaacc tccagggcga gatcaaggag
     1681 ccgtggggcg ggctgctgcc ggaaacgaag
//
LOCUS       DASVII010000579         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_278737,
            whole genome shotgun sequence.
ACCESSION   DASVII010000579 DASVII010000000
VERSION     DASVII010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278737"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VF309_07530"
     CDS             complement(<1..678)
                     /locus_tag="VF309_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7466474.1"
                     /translation="MPLKTTAGDHGQAVKGRGATINIEGRFEIWNREADDDGWFQDPG
                     DEPARVKTVISIEHAKSVISRHDSPDLGFSQSINPYRGCAHGCPFCYARPSHAYLGLS
                     PGLDFETRLTAKVNAAQKLREELAKPGYRCEPLAIGVNTDAYQPIERQQRITRSILEV
                     VHETSHPVSLITKSALIERDLDLLAPMAKKRLVAVTLSITTLDHEISRRMEPRTSAPA
                     RRLEAIRR"
     gene            870..1718
                     /locus_tag="VF309_07535"
     CDS             870..1718
                     /locus_tag="VF309_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7466475.1"
                     /translation="MIDGRKLVVVLPAFNAAGTLELTINDIPPGVVDEVILVDDRSND
                     QTVEVARKLGITKIHVHPENRGYGANQKTCYRAALAEGADIVVMLHPDYQYEPKLITA
                     IAGMIASGVYGVVLGSRILGGGSSGALAGGMPMWKYVANRALTAMQNLLTGAKLSEYH
                     TGYRAFSRRLLETLPLHENSDDFVFDNQMLIQTAFFGFSIAEVTCPTSYLPEASSIGF
                     GRSLVYGVGVLRVACDYRLAKLGLVKPRFLAKAGRTLRGAPGADARTRDLAPTAEKAA
                     ANRRDG"
BASE COUNT          328 a          566 c          561 g          329 t
ORIGIN      
        1 gcggcgaatg gcttcgagcc ttcttgccgg cgcgctggtg cgcggctcca tccttcgcga
       61 gatctcgtgg tccagggtcg taatcgaaag ggtcacggcg acaaggcgct tcttcgccat
      121 cggcgcgaga aggtcgagat cgcgctcgat caacgcggac ttggtgatca gcgacacagg
      181 atgacttgtt tcgtgcacga cctcgaggat cgatcgcgtg attcgctgct ggcgctcgat
      241 gggctggtac gcatcggtgt tcacgccgat tgcgaggggc tcgcatctgt agccgggctt
      301 cgcgagctct tcgcgcaact tttgcgcagc attcaccttt gcggtcaacc tcgtctcgaa
      361 atcgagcccg ggcgatagac cgagataggc gtgactcggc cgggcataac agaatgggca
      421 tccatgcgca catccccgat acggattgat cgactgcgag aatccgagat cgggagagtc
      481 gtggcgcgag atcacgctct tcgcgtgctc gatggaaatc acggtcttca cgcgcgcggg
      541 ctcgtcgccc ggatcctgga accatccgtc gtcgtcggcc tcgcgattcc agatctcgaa
      601 gcggccttcg atgttgattg tggcgccgcg gcccttcacg gcctggccat ggtctccggc
      661 ggtggttttc aatggcatga acgaaagtct acgcgtggaa atcgcgcgcg gggcgagcga
      721 agacaaacga gattcgcgat gtggataagc gccgctccca agcctccatc gatcgggtta
      781 tgcacaaatc caaaagcgcc gcggaccgtg gccatgccag aatgcaggcc aaggcggccc
      841 tttcaagggc gcgaggcgaa agcggatcca tgatcgatgg caggaaactg gtggtcgtgc
      901 tgccggcgtt caatgcggcc ggcacgctgg agctcaccat caacgacatt cccccgggcg
      961 tcgtcgacga ggtcatcctc gtcgacgatc gcagcaacga ccagacggtg gaagtcgcga
     1021 ggaagctcgg catcaccaag atccacgtcc atcccgagaa ccgcggctac ggcgcgaacc
     1081 agaagacctg ctaccgcgcc gcgcttgccg agggcgccga catcgtcgtg atgctgcacc
     1141 ccgactacca gtacgagccg aagctgatca ccgccatcgc cgggatgatc gcctccggag
     1201 tctatggcgt cgtgctgggc tcgcggatcc tcggcggcgg ctcctcgggc gcgctcgcgg
     1261 gcggcatgcc gatgtggaag tacgtcgcca accgcgcgct caccgcgatg cagaacctcc
     1321 tcaccggcgc gaagctttcc gagtaccaca ccggctaccg cgcgttctct cgcaggctgc
     1381 tcgagacgtt gccgctacat gaaaactccg acgatttcgt gttcgacaat cagatgctga
     1441 tccaaaccgc tttcttcggt ttcagcatcg ccgaagtgac gtgcccgacc tcctaccttc
     1501 ctgaagcgtc gtcgatcggc ttcgggcgca gtctcgtcta cggcgttggg gtgctgcgcg
     1561 tcgcgtgcga ctaccggttg gcgaagcttg ggctcgtcaa gccccggttc ctcgccaagg
     1621 ctggaagaac gctgcgtgga gccccgggcg ccgatgcacg gacgcgcgat cttgcgccaa
     1681 ccgcggaaaa agccgccgcc aaccgtcgag atggctgacg ccgtcgtcca gaacctggcc
     1741 tgaaaccagc gctacgactt ttgtgcgcgc aagcctggtt aagt
//
LOCUS       DASVII010000580         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_206425,
            whole genome shotgun sequence.
ACCESSION   DASVII010000580 DASVII010000000
VERSION     DASVII010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206425"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF309_07540"
     CDS             complement(<1..313)
                     /locus_tag="VF309_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX7466476.1"
                     /translation="MSRYVERAENTARVLDVTWRMSLLVQEPELQNQEWFAPLNITGT
                     LFPFSGRHDEVCAREVLHFMALDAENPGSIFSCARQARENARAVRGSITSEMWEVLNS
                     TW"
     gene            complement(380..1795)
                     /locus_tag="VF309_07545"
     CDS             complement(380..1795)
                     /locus_tag="VF309_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX7466477.1"
                     /translation="MAISGLYNEMFDADRSVRGHYAAFAEWLAETPPSRIAQNRHAAD
                     LLFHRVGITFAVYGEASGTERLIPFDIVPHVIPGAQWQRIARGLKQRVTALNRFLHDI
                     YHAQEIVKAGKVPSDQVNRNAQFRQEMVGVDVPGGVYAHVAGVDLVKHADGEYYVLED
                     NLRTPSGVSYMLENRKMMMRLFPELFAQQRIRPVDHYPDLLLETLRGVAQHKEANPTI
                     VLLTPGHYNSAYFEHAFLAQQMGIELVEGQDLFVLNNTVFMRTTQGPQKVDVIYRRID
                     DDFLDPLAFRPDSMLGVPGLLSAYRAGRVTLANAIGTGVADDKSMYPCVPEMIRFYMG
                     EEPILRNVPTYVLGKEEDCLYVLDHLEELVVKEAQGSGGYGMLVGPTSTRDERERYRQ
                     RILDQPGRFIAQPTLSLSMVPTYCGDVLAYRHIDLRPYVLTGSEVTFVPGGLTRVALR
                     EGSLVVNSSQGGGVKDTWVVD"
     gene            complement(1959..2162)
                     /locus_tag="VF309_07550"
     CDS             complement(1959..2162)
                     /locus_tag="VF309_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466478.1"
                     /translation="MEIVQVPVGALQASSRCELRSHRIGRVKFVGLPLEMDPGAALRG
                     QPPPGVVARIVSRVIGCLVAARP"
     gene            2264..>3366
                     /locus_tag="VF309_07555"
     CDS             2264..>3366
                     /locus_tag="VF309_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7466479.1"
                     /translation="MLSPSAATDLAPFEGELRSAFGAPHAELPARALQFARGASSAAG
                     REEPFERAVAVARLLLVQGADPETIAAALISQAIPERELDLDAVQAAFGEELTGLLRG
                     VARAGRIESLNASGVDLEQLRKMLLAIAEDVRVVLIKLAERVVYLRSLTRADEALRRA
                     AGRQTLELFAPLANRLGVSDVKWELEDFSFRYTEPELYKKIAQLLDEKRNDREAYIQR
                     VIAKLDAELAAMGIHAKIQGRPKHIYSIWRKMRGKNVSFEGLYDIRAVRVIVGNIREC
                     YTVLGVVHDLWTPIEGEFDDYIAQPKANEYKSLHTAVVGPEGKTLEVQIRTEEMHQHS
                     EHGVAAHWRYKEGSRADKAFDSKIAWLRQVLAW"
BASE COUNT          580 a         1117 c         1162 g          507 t
ORIGIN      
        1 gccaggtgga gttgagcacc tcccacatct ccgaggtgat cgagccgcgc accgcgcgcg
       61 cgttctcgcg cgcctggcgc gcgcaggaga agatcgaccc cgggttctcg gcatcgaggg
      121 ccatgaagtg cagcacctcg cgcgcgcaaa cctcgtcgtg gcgccccgag aaggggaaga
      181 gcgtgccggt gatgttgagg ggcgcgaacc actcctggtt ctgcagctcc ggctcctgca
      241 ccagcagcga catgcgccag gtgacgtcga gcacgcgcgc ggtgttctct gccctttcca
      301 cgtagcggct catccaatac agcgtcgatg cgactcttga gagcatctac ttggcgggca
      361 aagggctagg catttcggat caatcaacca cccacgtatc cttcacgccg ccgccctggg
      421 aggagttcac caccagcgag ccctcgcgaa gggccacgcg cgtgaggccg ccgggaacga
      481 aggtcacttc ggagccggtg aggacgtagg gcctgaggtc gatgtgccgg taggcgagca
      541 cgtcgccgca gtaggtcggc accatggaaa gggaaagcgt cggctgcgcg atgaagcgcc
      601 cgggctggtc gaggatcctc tggcgatagc gctcgcgctc gtcgcgggtc gaggtcggcc
      661 ccaccagcat gccgtagccg ccggagccct gcgcctcctt caccaccagc tcctcgaggt
      721 ggtcgagcac gtagaggcag tcctcctcct tgccgaggac gtaggtcggg acgttgcgca
      781 ggatcggctc ctcgcccatg tagaagcgga tcatctcggg cacgcacggg tacatcgact
      841 tgtcgtcggc cacgcccgtg ccgatcgcgt tggcgagggt cacgcgcccg gcgcgatagg
      901 cagagaggag tcccggaacg ccgagcatcg agtcgggacg gaaggcgagc gggtcgagga
      961 agtcgtcgtc gatgcgccgg tagatcacgt ccaccttctg cgggccctgc gtcgtgcgca
     1021 tgaacaccgt gttgttcagc acgaagaggt cctggccctc gaccagctcg atgcccatct
     1081 gctgcgcgag gaacgcgtgc tcgaaatacg cgctgttgta gtggcccggg gtgaggagga
     1141 cgatcgtcgg atttgcctcc ttgtgctgcg ccacgccccg cagggtctcg agcaggaggt
     1201 cggggtagtg gtcgaccggc ctgatgcgct gctgcgcgaa gagctcgggg aagagccgca
     1261 tcatcatctt gcggttctcg agcatgtacg agacgcccga gggcgtgcgc aggttgtcct
     1321 cgaggacgta gtactcgccg tcggcgtgct tcaccaggtc gacgcccgcg acgtgcgcgt
     1381 acaccccgcc cggaacgtcc acgccgacca tttcctggcg gaactgggcg ttgcggttca
     1441 cctggtcact cgggaccttt cccgccttca cgatctcctg cgcgtggtag atgtcgtgga
     1501 ggaatcgatt caacgcggtc acgcgctgct tcaggccccg cgcgatgcgc tgccactggg
     1561 cgccggggat cacgtgcgga acgatgtcga aagggatcag gcgctcggtg cccgaggcct
     1621 cgccgtagac cgcgaaggtg atgcccacgc gatggaaaag gaggtccgcc gcatggcggt
     1681 tctgggcaat gcgcgagggc ggcgtctcgg cgagccattc ggcgaacgcg gcgtaatgcc
     1741 cgcggaccga gcggtccgcg tcgaacatct cgttatagag cccggaaatt gccatctgac
     1801 aatgcgagga gaggggcggc gcgccgcggg acgaccgaaa tggccggcta ccggcaccat
     1861 cggtaccgtc agcaagccct gtgccacagg atagcaagaa gcgagaaaga ccggcctccg
     1921 gcgagtgccg gaggccggga agatccgcgc cgggacgcct agggccgcgc cgccaccagg
     1981 caaccgatga cgcgggatac gatgcgggcc accacgcccg ggggaggctg tccccgcagc
     2041 gccgctccgg gatccatctc gagcggcagg ccgacgaact tcacgcggcc gatgcgatgg
     2101 gagcgaagct cgcatcgcga cgaggcctga agggcgccaa ccgggacctg aacgatttcc
     2161 atgggagcat ggtggcctat cgtcccggag aggcgcccgg gaggattcgc gcatggacat
     2221 gccgaatttt cagcaagtag aatgaattca aggccactcc ctcgtgctca gcccttccgc
     2281 cgctaccgac ctcgccccct tcgaaggcga gctccgctcc gccttcggcg cgccccatgc
     2341 cgagctgccg gcgcgggcgc tgcagttcgc gcggggtgcc tccagcgccg ccggccgcga
     2401 ggagccgttc gagcgcgccg tggccgtggc gcgcctcctc ctggtgcagg gcgccgatcc
     2461 cgagacgatc gccgcggcgc tcatcagcca ggcgatcccc gagcgggagc tcgacctgga
     2521 cgccgtgcaa gcggcgttcg gcgaggagct cacgggcctg ctgcgcggcg tcgcgcgcgc
     2581 gggccgcatc gagtcgctca acgcgagcgg cgtcgatctc gagcagctgc gcaagatgct
     2641 cctcgcgatc gccgaggacg tgcgcgtggt cctcatcaag ctcgccgagc gcgtggtgta
     2701 cctgcgttcg ctcacccggg ccgacgaggc gctgcgccgc gccgccggcc ggcagaccct
     2761 cgagctgttc gccccgctcg ccaaccgcct gggcgtttcc gacgtgaagt gggagctcga
     2821 ggacttctcc tttcgctaca cggagcccga gctctacaag aagatcgcgc agctcctcga
     2881 cgagaagagg aacgatcgcg aggcctacat ccagcgcgtc atcgcgaagc tcgacgccga
     2941 gctcgcggcg atgggcatcc acgcgaagat ccagggccgg cccaagcaca tctactcgat
     3001 ctggcggaag atgcgcggca agaacgtgtc gttcgagggc ctctacgaca tccgcgcggt
     3061 gcgcgtcatc gtcggcaaca tccgcgagtg ctacacggtg ctcggcgtgg tgcacgacct
     3121 gtggacgccc atcgagggcg agttcgacga ctacatcgcc cagccgaagg cgaacgagta
     3181 caagtcgctg cacaccgccg tggtcggacc cgaaggcaag acgctcgagg tccagatccg
     3241 caccgaggag atgcaccagc actcggagca cggcgtcgcg gcgcactggc gctacaagga
     3301 aggctcccgc gccgacaagg cgttcgactc gaagatcgcg tggctgcgcc aggtgctcgc
     3361 gtggaa
//
LOCUS       DASVII010000581         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_98285, whole
            genome shotgun sequence.
ACCESSION   DASVII010000581 DASVII010000000
VERSION     DASVII010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98285"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..977
                     /locus_tag="VF309_07560"
     CDS             186..977
                     /locus_tag="VF309_07560"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX7466480.1"
                     /translation="MLPLAPLPDIAPLDQPSEKARPEDAKPDVVALAQPAVPVPALEP
                     PKFPLDALPASISIDYQLTSSFADGHAVYHWRRDGDNYTITSEAEAVGFFTLFLEGRI
                     LQESRGTVTAEGLRPRRFVERKPNTAAEGLEFDWAAHKVTFDRNNERKTAELADNTVD
                     WLSMIFQMAHVPPSGETYALRVYTQRKLYKFELRILGEEEVEIPLGKVKALHLRHVDP
                     EDQTVVDVWLGVQQHYLPVKLRYPVARNRLMVEQVATSVSGPWAR"
     gene            974..2233
                     /locus_tag="VF309_07565"
     CDS             974..2233
                     /locus_tag="VF309_07565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX7466481.1"
                     /translation="MNLARAQLDAIVHALAVVLPGNAPADRQLGQFFRDNKNLGSHDR
                     ALVADTVYAALRRRRYLEHVTPRASPREMALATIVKIQGVGLGQIESALRGDEKAWLT
                     ALKQESFDELPFEIRADLPDWVIERLRKQMSDEELLALARGLQQPARLDLRVNTMKAP
                     REAVLERLAYDEIAAHPTRYSPVGVRLREKPALNRHPMFLDGAVEVQDEGSQILGMLV
                     EPRRGEMVVDFCAGAGGKTLQMGAAMNSSGRLYAFDISDKRLANLAPRLKRSGLSNVF
                     PQRISGENDPKVKRLRGKIDRVLVDAPCTGLGTLRRNPDLKSRQTVEGLGELNAKQRA
                     ILAAAAALVKPGGRLVYGTCSLLSAENEDIVKEFLAAHADFHIVPAGEVLKRQGIAIP
                     GMGEYLRLYPHVHDTDGFFAAVMERDK"
     gene            2252..3565
                     /locus_tag="VF309_07570"
     CDS             2252..3565
                     /locus_tag="VF309_07570"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7466482.1"
                     /translation="MPLGLDISPLARVVVALAGGTTPREWFHQIAVAAGALFAAWAFA
                     RIACARVKVSPKWKFGEGDFEGVVFPALAWLFAWIGKSALERFESPDFLEIVLTLIAA
                     LAVIRVAAYVLRHVVPASALQRAVIRVVTWTAWIVAVLYVAGLLPEVVAALDAHGFSI
                     GKGKPAITILDLLKGATALFLSITLALYLSRVTESRVLSSDSMEMTTRVVIAKAVRVA
                     TLFIAIFIALPLAGIDVTTLSVFTGALGVGLAFGLQKITSNYVSGFIVLLDRSLRIGD
                     VVSVDGRRGEVTAIETRYTVIKGGDGVESIIPNEKMITEIVNHHTYSDSRVSVAMSVT
                     ISYDSDVDRAFELLVASARKQARVIADPPAIARVKQLSDRGVELELTVWISDPVAGEG
                     DLRSELLKDVLRAFRTGGIEIAFPRREVRLIATDETGETPSPPSA"
     gene            3684..>4042
                     /locus_tag="VF309_07575"
     CDS             3684..>4042
                     /locus_tag="VF309_07575"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7466483.1"
                     /translation="MFSGFLNLPWWGDLIAGLILVQITIAGVTVYLHRCQAHRALEVH
                     PVVSHFFRFWLWMTTGMITKEWAAIHRKHHAKCETEEDPHSPKIYGIKTVLLQGVDLY
                     RVASQEPATMEKYGLGTP"
BASE COUNT          675 a         1401 c         1327 g          639 t
ORIGIN      
        1 cgccccaacg agtggagggg attgcggagg aagcggcgcc cgcatacacg gcaagcctcg
       61 atggggcggc accttccgaa acgagcgccc ccgcgcgggc gccggcgtct cccaagcgtg
      121 cagcgcggcc gcgcgcgatc ccgcttccca ggttcgagcc gctggcctcg atcgacgagc
      181 cgcaattgct ccccctggcg ccgctgcccg acatcgcacc gctggatcag ccctccgaaa
      241 aggcgaggcc ggaggacgcg aagcccgatg tcgtcgccct ggcgcagccc gcggtgccgg
      301 tccccgccct cgagccgccg aaattcccgc tcgatgcgct tccggcgagc atctcgatcg
      361 actaccagct cacgtcgtcg ttcgccgacg gccacgcggt gtatcactgg cgccgcgacg
      421 gcgacaacta cacgatcacc agcgaggcgg aagcggtcgg attcttcacg ctcttcctcg
      481 agggacgcat cctccaggaa agccggggca cggtcaccgc cgaagggctc cgcccgcggc
      541 gcttcgtcga gcgcaagccc aacaccgcgg ccgaaggcct cgagttcgac tgggccgcgc
      601 acaaggtcac tttcgaccgc aacaacgaga ggaagaccgc cgagctcgcc gacaacaccg
      661 tcgactggct ctcgatgatc ttccagatgg cgcacgtgcc gccttcgggc gagacgtacg
      721 cgctgcgcgt ctacacgcag cgaaagctct acaagttcga gctccggatc ctcggcgagg
      781 aagaggtgga gattccgctc ggcaaggtga aggccctgca cctgcgccac gtcgacccgg
      841 aggaccagac ggtggtggac gtctggctcg gcgtgcaaca gcactacctt cccgtgaagc
      901 ttcgctatcc ggtcgcgcgc aatcggctca tggtcgagca ggtcgcgacg agcgtctcgg
      961 gcccctgggc gcgatgaacc tcgcccgcgc gcagctcgat gcgatcgtcc acgccctggc
     1021 cgtcgtcctg ccgggcaacg cgcccgccga ccgccagctg ggacagttct ttcgcgacaa
     1081 caagaatctc ggcagccacg atcgcgccct ggtggccgac accgtgtacg cggcgcttcg
     1141 ccgccgccga tatctcgagc acgtcacgcc ccgggcctcg cctcgcgaga tggccctcgc
     1201 caccatcgtg aagatccagg gtgtcggact cggccagatc gaatcggcgc tgcgcggcga
     1261 cgagaaagcg tggctcacgg cgctcaagca ggagagcttc gacgagctgc ccttcgagat
     1321 ccgcgccgat cttcccgatt gggtgatcga gcggcttcgc aagcagatga gcgacgagga
     1381 gctgctcgcg ctcgcgcgcg gtttgcagca gcccgcgcgc ctcgacctgc gcgtgaacac
     1441 gatgaaggcg ccgcgcgaag cggtgctcga acgcctcgcc tacgacgaga tcgcggccca
     1501 tcccacgcgc tattcgccgg tcggcgtgcg cctgcgcgag aagcccgcgc tcaaccggca
     1561 tccgatgttc ctcgacggcg ccgtcgaggt gcaggacgag ggcagccaga tcctcggcat
     1621 gctggtcgag ccgcggcgcg gcgagatggt ggtcgacttc tgcgcgggag ccggcggcaa
     1681 gacactgcag atgggcgcgg cgatgaattc gtcgggacgc ctctacgcgt tcgacatttc
     1741 cgacaagcgc ctcgccaatc tcgcgccgcg cctgaagcgc tccggcctgt cgaacgtgtt
     1801 cccgcagcgc atctccggcg agaacgaccc gaaggtgaag cggctgcgcg gcaagatcga
     1861 ccgcgtcctg gtcgacgcgc cgtgcacggg cctcggcacc ttgaggcgca accccgacct
     1921 gaagtcgcgc cagaccgtcg aaggcctcgg cgagctgaac gccaagcagc gcgcgatcct
     1981 cgccgcggcc gcggcgctgg tgaagccggg cgggcgcctc gtgtacggga cgtgcagcct
     2041 gctctccgcg gagaacgagg acatcgtcaa ggagttcctc gccgcccacg ccgatttcca
     2101 catcgtgccg gcgggcgagg tgctgaagcg ccagggcatc gcgattcccg ggatggggga
     2161 gtacctgcgc ctctatccgc acgtccacga caccgacgga tttttcgccg cggtgatgga
     2221 acgcgataag tagccgcggg ccggcgactc catgccactg ggtctcgaca tctcccctct
     2281 cgcgcgcgtc gtcgtggcgc tcgccggcgg cacgacgccc cgggaatggt tccaccagat
     2341 cgcggtcgcc gcgggcgccc tcttcgcggc gtgggcgttc gcgcgcatcg cgtgcgcgcg
     2401 ggtgaaggtg agtcccaagt ggaagttcgg cgagggcgac ttcgagggcg tcgtcttccc
     2461 cgcgctcgcc tggctgttcg cgtggatcgg caagagcgct ctcgagcgct tcgagtcgcc
     2521 ggatttcctg gagatcgtgc tgacgctcat cgccgcgctg gcggtgatcc gcgtcgccgc
     2581 gtatgtcctg cggcacgtgg tccccgcaag cgccctgcag cgcgcggtga tccgcgtcgt
     2641 cacgtggacg gcgtggatcg tcgcggtgct gtacgtggcg ggcctgcttc ccgaggtcgt
     2701 ggcggcgctc gacgcccatg ggttcagcat cggcaagggg aagcccgcga tcacgatcct
     2761 agacctgctg aagggcgcga cggcgctctt cctttccatc acgctcgcgc tctacctctc
     2821 ccgcgtgacc gaaagccgcg tgctctcctc ggactcgatg gagatgacca cgcgcgtcgt
     2881 gatcgccaag gcggtgcgcg tcgcgacgct cttcatcgcg atcttcatcg cgctgccgct
     2941 cgcgggcatc gacgtcacca cgctctcggt cttcaccggc gcgctcggcg tgggcctcgc
     3001 cttcggcctg cagaagatca ccagcaacta cgtgagcggc ttcatcgtgc tgctcgaccg
     3061 cagccttcgc atcggcgacg tggtgagcgt cgacggccgc cgcggcgagg tgacggcgat
     3121 cgagacgcgc tacacggtca tcaagggcgg cgacggcgtc gagtcgatca tccccaacga
     3181 gaagatgatc accgagatcg tcaaccacca cacctacagc gactccaggg tgtccgtggc
     3241 gatgtcggtg accatttcgt acgacagcga cgtggaccgc gccttcgagc tcctcgtggc
     3301 gagcgcccgg aagcaggcgc gcgtgatcgc cgatccgccc gcgatcgcgc gcgtgaagca
     3361 gctttccgat cgcggcgtgg agctcgaatt gacggtatgg atttccgatc ccgtcgcggg
     3421 cgaaggcgac ctgcgcagcg agctgctgaa ggacgtgctg agggctttcc ggacgggggg
     3481 aatcgagatc gccttcccgc gccgcgaagt gcgcctcatc gctaccgatg aaacgggaga
     3541 aaccccttct ccaccaagcg cttagagggg cccggcaaaa gggggcacct cgctgtttgc
     3601 gatgggctac aatcgctggg attcatcccc atataagagc ctgcagcccg acatagcggc
     3661 tcactccaaa aggcacacat tcaatgttca gcggattcct gaatcttccc tggtggggcg
     3721 acctgatcgc cgggctcatc ctggtgcaga tcaccatcgc cggcgtgacc gtctacctgc
     3781 atcgctgcca ggcccaccgc gcgctcgagg tgcatccggt cgtgagccat ttcttccgct
     3841 tctggctgtg gatgaccacg ggaatgatca ccaaggaatg ggccgcgatc catcgcaagc
     3901 accacgccaa gtgcgagacc gaggaagacc cgcacagtcc gaagatctat ggcatcaaga
     3961 ccgtcttgct gcaaggggtg gacttatacc gtgtcgcctc gcaggaaccg gcgacgatgg
     4021 aaaaatacgg tctcggcacg cc
//
LOCUS       DASVII010000582         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_206496,
            whole genome shotgun sequence.
ACCESSION   DASVII010000582 DASVII010000000
VERSION     DASVII010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206496"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="accC"
                     /locus_tag="VF309_07580"
     CDS             <1..1270
                     /gene="accC"
                     /locus_tag="VF309_07580"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7466484.1"
                     /translation="VAVHSEADADAKYVMLADESVCIGPAPSRDSYLNIPAIISAAEV
                     TDAQAIHPGYGFLSENADFAERVENSGFVFIGPRADSIRTMGDKIQAKVTMKKAGIPC
                     VPGSEGPLPDDPKEIRRLAAAVGYPVIIKAAGGGGGRGMRVVHTEAALVNAVTMTKTE
                     AQNAFNNPTVYLEKFLENPRHIEIQVLADEHKQAIYLGERDCSMQRRHQKIIEEGPAP
                     LVKPRLIERIGERCAEACRKMGYRGAGTFEFLYEKDEFYFIEMNTRVQVEHPVTELIT
                     GVDIVQEQIRVAAGMKLKYRQKDIVKKGHAIECRINAENPYTFVPSPGRITQYHVAGG
                     PGIRYDSHCYANYYVPPYYDSLIGKLIAYGDTREQAIARMRVALSEMVIEGIQTNIPL
                     HQELMNDAAFIRGGVSIHYLEEKIAALKSI"
     gene            1308..2198
                     /gene="prmA"
                     /locus_tag="VF309_07585"
     CDS             1308..2198
                     /gene="prmA"
                     /locus_tag="VF309_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7466485.1"
                     /translation="MAWQRVDLVVEGGEAEALADALEEGGAISSEISDADAGTPTERA
                     IFGEPGADAGLWPRTRVSALFRSDAPVARIVDKALESCSVTALAAASLSRVDDEDWVT
                     LTQRQFAPIRAGERLWIVPTWHDPPEPGAINIALDPGAAFGTGSHPTTRLCLAWLEKN
                     VAAGDTVLDYGCGSGILAIAALKLGAAAATGVDIDPRALEAARYNSQRNGVALEVMTA
                     QGALPRAARITVANILANPLRMLAPILASLTQPGGLIALSGILDEQADEVCRAYAPWS
                     RLAVSARQDGWVLLAGRKER"
     gene            2202..3080
                     /locus_tag="VF309_07590"
     CDS             2202..3080
                     /locus_tag="VF309_07590"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEX7466486.1"
                     /translation="MLVTTCTQCRSRFRVTPTQLNAKQGQVRCGRCHGVFNGFEALER
                     FPDDDTGARLLAAREAVERAAGISPPIEGLDFDELPDIETLQSPVTPSQEDATPFVEA
                     PAPVPSTPPAPRRPVPRAPPADVALPPLVRAEPPARAWAFGAALLCLVFAAELAYAYR
                     GNLAQRYPVVRPYLESACAAAGCTVSWAREDAMLKLEDSELLEVPGKPTEIALGAHIR
                     NLAQVAEEYPHLELTLTDLTGQAAIRRVLRPTDYLGRPVGAGETIAPGAELSVQLRLE
                     TPRIKATGYELLLFYP"
     gene            complement(3071..>3833)
                     /locus_tag="VF309_07595"
     CDS             complement(3071..>3833)
                     /locus_tag="VF309_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX7466487.1"
                     /translation="LESRAGVPPAVREMLNETWVRALVRVHRTEGEGSSAWQGMLLGM
                     EDLLWSVEPKATADDRKRLITMLPGMLRTLHEGLVRGELDAKGRGAFLGVLVDCHAMA
                     VKAGMRGLAVVAEPAPVVVSQDPSIERAVIPAGGVQVEEIRLRAPRGGAVRNVFTRTG
                     VWTNLQRGTWVEFARESGGASRSRLTWISPNKGVYLFTNPSSPNAAVSISPEALAQQL
                     RMGEAKIIDDASIVDRAVDTMLTSLRAEAPAPLQG"
BASE COUNT          621 a         1354 c         1292 g          566 t
ORIGIN      
        1 ggtcgcggtg cattcggagg ccgacgccga cgcgaagtac gtcatgctcg ccgacgagtc
       61 ggtttgcatc ggcccggctc cctcgcgcga cagctatctc aacatccccg cgatcatcag
      121 cgccgccgag gtcacggacg cgcaggcgat ccatccgggc tacgggtttc tgtccgagaa
      181 cgccgacttc gccgagcgcg tcgagaactc cggcttcgtc ttcatcgggc cgcgcgcgga
      241 ttcgattcgc acgatgggcg acaagatcca ggcgaaggtg acgatgaaga aggcaggaat
      301 cccttgcgtg ccgggctccg aggggccgct gcccgacgat cccaaggaga tccgccggct
      361 cgccgccgcc gtcggctatc cggtgatcat caaggccgcc ggcggcggcg gcgggcgcgg
      421 catgcgggtg gtgcacaccg aggcggcgct ggtgaacgcg gtcacgatga ccaagaccga
      481 ggcgcagaac gcgttcaaca atcccaccgt ctacctcgag aagttcctcg agaacccgcg
      541 ccacatcgag atccaggttc tcgccgacga gcacaagcag gcgatctacc tcggcgagcg
      601 cgattgctcg atgcagcgcc gccaccagaa gatcatagag gaagggcccg cgcccctggt
      661 gaagccgcgc ctcatcgagc ggatcggcga gcgttgcgcc gaggcgtgcc gcaagatggg
      721 ctatcgcggc gcaggcacgt tcgaattcct ctacgagaag gacgagttct acttcatcga
      781 gatgaacacg cgcgtgcagg tcgagcatcc ggtgaccgag ctcatcacgg gcgtggacat
      841 cgtgcaggag cagattcgcg tggccgcggg catgaagctc aagtaccggc agaaggacat
      901 cgtgaagaag ggccacgcga tcgagtgccg gatcaacgcg gagaacccct acacgttcgt
      961 gccgtcgccc gggcggatca cgcagtatca cgttgccggg ggccccggca tccgctacga
     1021 ctcgcactgc tacgccaact actacgtgcc tccgtactac gattcgctca tcggcaagct
     1081 catcgcatac ggcgacacgc gcgagcaggc gatcgcgcgc atgcgcgtgg cgctctccga
     1141 gatggtgatc gagggcatcc agaccaacat cccgctgcac caggagctca tgaacgacgc
     1201 cgccttcatc cgcggcgggg tgagcatcca ttacctcgag gaaaagatcg cggcgctgaa
     1261 gtcgatctga tcgggcgcgc ggcacgatgg caggagggag acaagacgtg gcgtggcagc
     1321 gggtcgatct cgtcgtcgag ggaggggagg ccgaagcgct cgccgatgcc ctggaggaag
     1381 ggggcgcgat ctccagcgag atctcggacg ccgacgccgg aacgccgacg gagcgcgcga
     1441 tcttcggcga gcccggcgcc gacgcggggc tctggccgcg cacccgcgtg agcgcgctgt
     1501 tccgctccga cgcgccggtc gcgcgcatcg tcgacaaggc cctcgaaagc tgctccgtca
     1561 cggcccttgc cgccgcctcg ctctctcgcg tcgacgacga ggactgggtg acgctcacgc
     1621 agcgccaatt cgccccgatc cgcgccgggg aacggctgtg gatcgttccg acgtggcacg
     1681 accctcccga gcccggcgcc atcaacatcg cgctggatcc gggcgcggcg ttcggcaccg
     1741 gcagccatcc cacgacgcgg ctgtgcctcg cctggctcga gaagaacgtc gcggccggcg
     1801 acacggtgct cgactacggt tgcggctcgg gaatcctcgc catcgcggcg ctcaagctcg
     1861 gcgcggcggc ggcgacgggc gtcgacatcg atccgcgcgc gctcgaagcg gcgcgctaca
     1921 attcacagcg caacggcgtg gcgctcgaag tgatgactgc gcaaggcgca ctcccgcgcg
     1981 cggcccggat cacggtcgcc aacatactcg ccaacccttt gcgaatgctc gcgccgatcc
     2041 tcgcttcact cacgcagccc ggtggactca tcgccctttc gggaatcctc gacgagcagg
     2101 cggacgaagt ctgccgggcg tacgcgccat ggtcgcgact tgccgtgagc gcacgccagg
     2161 acggctgggt cctcctcgcc ggaaggaagg agcgctagcc gatgctcgtc accacttgca
     2221 cgcagtgccg ctcccgcttt cgggtgacgc ccacgcagct gaacgccaag caggggcagg
     2281 tgcggtgcgg gcgttgccat ggcgtcttca acggattcga ggcgctggag cgtttccccg
     2341 acgacgacac cggcgcgcgc ctgctcgcgg cacgcgaagc ggtggagcgc gcggcgggga
     2401 tttcgccccc gatcgagggg ctggatttcg acgagctgcc ggatatcgag accctgcagt
     2461 cgcctgtcac gccttcccag gaagatgcga caccgttcgt cgaggcgccc gcgccggtcc
     2521 cgtcgactcc gccggcgcca cgacggcccg tgcctcgagc accccccgcg gacgtcgccc
     2581 tgccgcccct tgtgcgcgcc gagccgccgg cccgcgcgtg ggcattcggc gccgcgctgc
     2641 tctgcctcgt attcgccgcc gagctcgcgt acgcctatcg gggaaatctc gcgcaacgct
     2701 atccggtggt gcggccctat ctcgaaagcg cttgcgcggc cgcgggttgc acggtctcgt
     2761 gggctcgcga agacgcgatg ctgaagctcg aggactcgga gcttctcgaa gtcccgggca
     2821 agcccaccga gatcgcgctc ggtgcccaca tccgcaacct cgcgcaggtc gccgaggaat
     2881 atccgcacct cgagctcacg ttgacggatc tcacgggcca ggccgcgatc cgccgcgtgt
     2941 tgcgaccgac cgattacctc ggccggccgg tgggcgcggg cgagacgatc gcgccgggcg
     3001 ccgagctcag cgtgcagctt cgcctcgaga cgccgcgcat caaggcgacg ggctacgagc
     3061 tgctgctctt ctatccctga aggggggcgg gcgcttccgc gcgcaggctc gtcaacatcg
     3121 tgtccaccgc gcggtcgacg atcgacgcgt cgtcgatgat cttcgcctcg cccatgcgca
     3181 gctgctgcgc gagcgcttcg ggagagatcg aaaccgccgc gttgggcgag ctcgggttgg
     3241 tgaacaggta gacgcccttg ttggggctga tccacgtgag gcgcgagcgc gacgctcctc
     3301 cgctctccct cgcgaattcg acccaggtgc cgcgctggag gttggtccag acgccggttc
     3361 gcgtgaagac attgcgtacc gccccgccgc gcggcgcgcg caggcggatc tcctcgacct
     3421 ggacgccgcc ggcgggaatc acggcgcgct cgatcgaagg gtcctggctc acgaccaccg
     3481 gcgccggctc ggcgacgacc gcgaggccgc gcatccccgc cttcaccgcc atcgcgtggc
     3541 aatcgacgag cacgccgagg aaagcgccgc gacccttcgc gtcgagctcg ccgcgcacga
     3601 ggccttcgtg cagcgtgcgc agcatgcccg gcagcatcgt gatgaggcgc ttgcggtcat
     3661 ccgcggtggc cttcggctcc acgctccaca gcaggtcttc catcccgagg agcatcccct
     3721 gccacgcgct ggagccttcg ccttcggtgc gatgcacgcg cacgagcgcg cgcacccacg
     3781 tttcgttcag catctcccgc accgccgggg gcacgcccgc gcgcgactcg agg
//
LOCUS       DASVII010000583         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_98440, whole
            genome shotgun sequence.
ACCESSION   DASVII010000583 DASVII010000000
VERSION     DASVII010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98440"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1312
                     /gene="dapC"
                     /locus_tag="VF309_07600"
     CDS             89..1312
                     /gene="dapC"
                     /locus_tag="VF309_07600"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEX7466488.1"
                     /translation="MNPRLELLQPYAFEKLRALMAGVAPPAGLRHINLSIGEPQHPTP
                     ALIKDALTANLASLARYPVTRGLPELRSAIAQWLARRHDLASIDPETQVLPVLGSREA
                     IFSIAQVVLDPAERDALVVCPNPFYQIYEGATLLGGATPYFVNAFAANGFRSDWDAVP
                     ADVWKRTRLLYACSPGNPNGRVMPRDEWRKLFELSDRHGFVIVSDECYSEIYFDEASP
                     PPGALGVARSLGRDGYPRLIVMGSLSKRSNAPGLRSGFAAGDASLMERFHAYRTYHGC
                     AMSNAVQHASIAAWKDERHVAENRRLYREKFDAFHAIVNPALPLTRPQAAFYFWAAVP
                     GDDTVFARELLAATNMTVLPGSFMSRDAHGANPGRGFVRIALVSTLEETLEAARRLEG
                     FAARLATARAGMTIP"
     gene            1309..>2132
                     /gene="dapD"
                     /locus_tag="VF309_07605"
     CDS             1309..>2132
                     /gene="dapD"
                     /locus_tag="VF309_07605"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX7466489.1"
                     /translation="MKGTAHPLQAAIDKAFERRAEFSPGSAPVAVKKAVARVLAELDA
                     GKLRVAEKLDGEWITHQWIKKAVLLSFRLRDNEIVDGGHTTYYDKVDSKFAHFTLADF
                     AAGGYRVVPPAHARRGAFIGKNVVLMPSYVNVGAYVDEGTMVDTWATVGSCAQIGKNV
                     HLSGGVGIGGVLEPIQANPTIIEDNCFIGARSEIVEGVIVGEGSVVSMGVYIGQSTKI
                     YNRATGEVSYGKIPPGSVVVSGSLPGGDGRYSLYCAVIIKQVDAKTRAKTGINELLR"
BASE COUNT          342 a          764 c          688 g          338 t
ORIGIN      
        1 ggatgcatcg gcacgccgat gctcgctgcc gaaattcgta cctgacacca atttccggcg
       61 ccaaattccg cccccgtata attgggggat gaatccccgc ctcgagctgc tgcagcccta
      121 cgccttcgaa aagctgcgcg cgctgatggc cggcgtcgcc ccgcccgcgg gcctgcgcca
      181 catcaacctg tccatcggcg agccgcagca tccgacgccc gcgctcatca aggacgcgct
      241 caccgcgaat ctcgcgagcc tcgcgcgcta tccggtgacg cgaggccttc ccgagctgcg
      301 ttccgcgatc gcgcagtggc tcgcgcgccg gcacgacctg gcctcgatcg atccggaaac
      361 ccaggtgttg ccggtgctcg gctcgcgcga ggcgatcttc tcgatcgcgc aggttgtgct
      421 ggatccggcg gagcgcgacg cgctcgtcgt atgccccaat ccgttctacc agatctacga
      481 gggagcgacg ctcctcggcg gggcgacccc gtatttcgtg aacgccttcg ccgccaacgg
      541 ctttcgctcc gactgggacg cggtgccggc cgacgtctgg aagcgcacgc gccttctcta
      601 cgcctgctcg cccggcaatc ccaacgggcg cgtgatgccg cgcgacgaat ggcgcaagct
      661 cttcgagctc tccgaccgcc acggcttcgt catcgtctcc gacgagtgct acagcgagat
      721 ctacttcgac gaggcgtcgc cgccgccggg cgcgctcggg gtggcccgct cgctcggacg
      781 cgacggctac ccgcgcctca tcgtcatggg atcgctttcg aagcgctcca acgcgccggg
      841 cctgcgctcg ggattcgccg cgggcgatgc gagcctcatg gagcgcttcc acgcgtaccg
      901 cacctaccac ggctgcgcga tgtcgaacgc cgtgcagcac gcttccatcg ccgcgtggaa
      961 ggacgagcgc cacgtcgccg agaaccgccg cctctaccgc gagaaattcg acgcgttcca
     1021 cgccatcgtg aacccggcgc tgccgctcac ccgcccccag gcggccttct acttctgggc
     1081 cgcggtcccg ggcgacgaca ccgtgttcgc gcgcgagctc ctcgcggcga cgaacatgac
     1141 ggtgctcccc ggaagcttca tgtcgcgcga cgcccacggc gcgaatcccg gccgcggctt
     1201 cgtgcgcatc gcgctggtgt cgacgctcga ggagacgctc gaagccgccc gccgcctcga
     1261 gggattcgcc gctcgcctcg ccaccgcccg cgccgggatg acgatcccat gaagggtacg
     1321 gcccatcccc tgcaggccgc catcgacaag gcgttcgagc gccgcgcgga attttctccc
     1381 ggctcggcgc ccgtcgccgt gaagaaggcc gtcgcgaggg tgctcgccga gctcgacgcg
     1441 ggcaagctgc gcgtcgccga gaagctcgat ggcgaatgga tcacgcacca gtggatcaag
     1501 aaggcggtgc tcctctcctt ccggctgcgc gacaacgaga tcgtcgacgg gggccacacg
     1561 acctactacg acaaggtcga ctccaagttc gcgcacttca cgctcgccga tttcgccgcg
     1621 ggcggctacc gagtggttcc cccggcgcat gcgcgccgcg gcgcgttcat cggcaagaac
     1681 gtggtgctga tgccctccta cgtgaacgtc ggcgcgtacg tggacgaggg caccatggtc
     1741 gacacgtggg cgaccgtggg ctcgtgcgcg cagatcggaa agaacgtcca cctgtcggga
     1801 ggcgtgggca tcggcggcgt cctcgagccc atccaggcga atcccaccat catcgaggac
     1861 aactgcttca tcggcgcgag gagcgagatc gtcgagggcg tgatcgtggg cgaaggctcg
     1921 gtggtctcga tgggcgtcta catcggccag agcaccaaga tctacaaccg cgccacgggc
     1981 gaggtgtcct acgggaaaat tccgccggga agcgtcgtag tttccggcag cttgcccggg
     2041 ggggatggcc ggtatagtct ctactgcgcg gtcatcatca agcaggtcga cgcgaaaacc
     2101 cgtgccaaga cggggatcaa cgaactgctc cg
//
LOCUS       DASVII010000584         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_458508,
            whole genome shotgun sequence.
ACCESSION   DASVII010000584 DASVII010000000
VERSION     DASVII010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458508"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1791
                     /gene="uvrA"
                     /locus_tag="VF309_07610"
     CDS             <1..>1791
                     /gene="uvrA"
                     /locus_tag="VF309_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7466490.1"
                     /translation="ARVGDPYCPEHNVPLSAQSIAQMVDHVTALEAGMRVMILAPLVV
                     GRKGEQESLIEELRAQGFVRLRIDGEVHEIDQLPKLDRNKKHTIEIVIDRLKVEAADP
                     ARPRAQPDGLKQRLAESFETALRHADGRAIAVEMESGKEHLFSAKFACPICSYSIPEL
                     EPRLFSFNNPMGACPRCDGLGQITFFDPKRVVAFPHLSLAGGAIRGWDRRNQFYFSML
                     TSLAQHYGFDVETPFESLPERAQEIVLDGSGEEKIAFRYPGEKGRSAVKEHAFEGIVP
                     NLERRHRETDSLAVKEELSKYLNTRDCPECHGTRLRREARHVFVAGRSIHELSGMPLK
                     EAQPFFERLELAGAKKPVAEKIVREIANRIQFLNDVGLDYLSLTRSADTLSGGEAQRI
                     RLASQIGSGLTGVMYVLDEPSIGLHQRDNARLIATLKRLRDLGNSVIVVEHDEEAIEA
                     ADYVIDMGPGAGVHGGEVVAHGTPDEVMANPASLTGQYLSGRKAIPTPTRRRRGDPKK
                     VLRIAGARGNNLKNVTVDIPVGLFVAVTGLSGSGKSTLINDTLYTAVAHHLYGSAAEP
                     ADHDAIEGLDFFDKVINVDQSPIGRTPRSNP"
BASE COUNT          310 a          614 c          602 g          265 t
ORIGIN      
        1 gcgcgcgtgg gcgatcccta ttgtcccgag cacaacgtgc cgctttccgc gcagagcatc
       61 gcgcagatgg tggaccacgt gacggcgctc gaggcgggca tgcgcgtgat gatcctcgcg
      121 cccctcgtcg tcggccgcaa gggcgagcag gagtccctca tcgaggagtt gcgcgcgcag
      181 gggttcgtgc gattgcggat cgacggcgag gtccacgaga tcgaccagct gccgaagctc
      241 gaccgcaaca agaagcacac catcgagatc gtgatcgacc ggctgaaggt cgaggcggcg
      301 gaccccgcgc ggccgcgcgc gcagcccgac ggattgaagc agcgcctcgc cgaatcgttc
      361 gagaccgcgc ttcgccatgc cgacgggcgc gcgatcgcgg tcgagatgga gtcgggcaag
      421 gagcacctgt tctccgcgaa gttcgcatgc cccatctgca gctactcgat tccggagctc
      481 gagccgcggc tcttctcgtt caacaacccg atgggagcct gcccgcgctg cgacgggctg
      541 ggccagatca cgttcttcga tccgaagcgc gtcgtcgcct ttccgcacct gtccctcgcg
      601 ggcggcgcga tccgcggctg ggaccggcgc aaccagttct acttctcgat gctcacctcg
      661 ctcgcgcagc actatggttt cgacgtggag acgcccttcg aatccctgcc ggagcgcgcc
      721 caggagatcg tcctcgacgg ctccggcgag gagaagatcg cgttccgcta tcccggcgag
      781 aagggcagga gcgcggtgaa ggagcacgcc ttcgagggca tcgtcccgaa cctcgagcgg
      841 cgccatcgcg agaccgattc gctcgcggtg aaggaggagc tgtccaagta cctcaacacg
      901 cgcgactgcc ccgaatgcca tggcacgcgc ctcaggcgcg aagcccgcca cgtgttcgtg
      961 gcggggcgca gcatccacga gctctcgggc atgccgctga aggaggcgca gcccttcttc
     1021 gagcgcctcg agctcgccgg cgcgaagaag ccggtcgccg agaagatcgt gcgcgagatc
     1081 gcgaaccgca tccagttcct gaacgacgtc gggctggatt atctgtccct cacgcgctcg
     1141 gccgacactc tctcaggtgg agaggctcag cgaataaggc tcgcgagcca gatcggctcg
     1201 ggcctcaccg gcgtgatgta cgtgctcgac gagccttcga tcggcctgca ccagcgcgac
     1261 aacgcgcgcc tcatcgcgac gctgaagcgc ctgcgcgacc tcggcaactc ggtgatcgtg
     1321 gtggagcacg acgaggaggc gatcgaggcc gccgactacg tgatcgacat ggggccgggc
     1381 gccggggtgc acggcggcga agtggtggcg cacgggacgc ccgacgaggt gatggccaat
     1441 ccggcatcgc tcaccggcca gtacctttcg ggacgcaagg cgattcccac gccgacgcgc
     1501 cggcgccgcg gcgacccgaa gaaggtgctg cgcatcgcgg gcgcgcgcgg caacaacctc
     1561 aagaacgtca cggtcgacat cccggtgggc ctgttcgtcg cggtgacggg gctttcgggc
     1621 tcgggcaagt cgacgctcat caacgacacg ctctacaccg cggtcgcgca tcacctgtac
     1681 ggctccgccg cggagcccgc ggaccacgac gcgatcgagg gcctcgactt cttcgacaag
     1741 gtgatcaacg tcgaccagag ccccatcgga cggacgcctc gctccaatcc g
//
LOCUS       DASVII010000585         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_458517,
            whole genome shotgun sequence.
ACCESSION   DASVII010000585 DASVII010000000
VERSION     DASVII010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458517"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VF309_07615"
     CDS             complement(<1..1175)
                     /locus_tag="VF309_07615"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX7466491.1"
                     /translation="MKKTKNDARDAANIVEALRKSTIGKVKVAVSDIDGILRGKYIHR
                     DKFFSAVEGGFGFCDVVFGWDSGDQCYDNTKLTGWQHGFPDALARIDLGTYRTVPWDG
                     GVPFFLGNFVTHDGKPHPLCPRQTLKRVLKRGEKLGFTAMVGSEFEFFNFSETPHTWA
                     EKKGVGPTPISPGMFGYSLLRANLNRDYFNALMDEMALFGVPIEGLHTETGPGVYEAA
                     ILFSEALEAADRAILFKAGAKEIGARFGIMPSFMAKWSAQYPGCSGHLHQSLSDGAKN
                     LFHDPKGRHGGMSKLFESYLAGQVAGLMELAPMYWPTINSYKRLVDGFWAPVTPTWGV
                     DNRTASFRVIAGSPKATRLETRCPGADMNPYLATAAVIAAGLNGIEKGMKLTTKPITG
                     "
     gene            complement(1172..1726)
                     /locus_tag="VF309_07620"
     CDS             complement(1172..1726)
                     /locus_tag="VF309_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7466492.1"
                     /translation="MSLKLGDIEKVFRDHGHIAYSGEGVTQLEHALQAAQLAEQAGAA
                     DALVAASLLHDLGHLLNQQGDTPTARGIDDQHQYVAIPFIRALFPPAIVEAIRLHVDA
                     KRALCAIDPDYYEALSEDSKRSLTLQGGIFSPEETDGFLAKPFAEDAMRVRRWDDAAK
                     VAGLQTASLAHFLEIAGRCTEKGS"
     gene            complement(1729..>3418)
                     /locus_tag="VF309_07625"
     CDS             complement(1729..>3418)
                     /locus_tag="VF309_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX7466493.1"
                     /translation="NLLAGVALLVWGTHIVRSGVLRIFGADLRRLLSKSTSNRGSAFL
                     AGLAVTGVVQSSSATALIASSFVSQNLIGLSAALAVMLGADVGTALMSQVFSLDLSWL
                     SPLLIILGVTFFLSKRNSRTGQMGRVAIGLGLMLLALQLVMLATRPITEAQGVRVIFA
                     TLSGDTMLDMLIGAAFAVLSWSSLAVVLIVATLAATKVVSVPVALCLVLGANLGSGIL
                     GLIVNARTPGAGRRVAFGNLLFKVAGCGAFSLALPWALEWIARFDPDARRQVLDFHVL
                     FNLTLAVVFIGFTDRVAGLVQKWLPESTERRQTAPRFLDAAALGTPALALANAARETL
                     RIGDTIEQMLNGVLEVIRTNDVSRVDAIIKLDDDVDRLYTAVKLYLTQVSREALDERD
                     GRRWTEIISLTINLEHVGDILERMLQDLRDKKIAQRLSFSEAGMREITELHEKLVVNL
                     RLGLSVFLNGDVKSAQQLLVEKERFRDLERAYHDSHLDRLAGQTLQSIETSSLHLDLI
                     SDMRRINSFFCSTAYPILEQAGQLRKSRLMGATGIYQARPDFAPPANELAGRTK"
BASE COUNT          561 a         1081 c         1228 g          548 t
ORIGIN      
        1 ccggtgatcg gcttcgtcgt gagcttcatc cccttctcga tgccgttcaa gcccgcggcg
       61 atcacggcgg cggtcgcgag atagggattc atgtcggccc cagggcagcg cgtctccagg
      121 cgcgtcgcct tgggggagcc cgcgatcacg cggaaggagg cggtgcggtt gtccacgccc
      181 caggtgggcg tcaccggcgc ccagaacccg tcgacgagtc gcttgtacga gttgatcgtg
      241 ggccagtaca tgggtgccag ctccatgagg cccgcgacct gccccgcgag atagctctcg
      301 aagagcttcg acatcccgcc gtggcggccc ttcggatcgt ggaagagatt cttcgcgccg
      361 tcggagaggc tctggtgaag gtgccccgag cagccgggat attgcgcgct ccacttcgcc
      421 atgaagctcg gcatgatgcc gaagcgcgcg ccgatctcct tggcgccggc cttgaagagg
      481 atcgcgcggt ccgcggcctc cagcgcctcg gagaagagga tggccgcttc gtacactccc
      541 gggccggttt cggtgtgcag gccctcgatc ggcacgccga agagcgccat ctcgtccatc
      601 agcgcgttga agtagtcgcg gttcaggttc gcgcgcagca gcgaatagcc gaacattccc
      661 ggcgagatcg gcgtcgggcc cacgcccttc ttctccgccc aggtgtgggg cgtctccgag
      721 aaattgaaga actcgaactc ggatccgacc atcgcggtga agccgagctt ctcgccgcgc
      781 ttgaggacgc gcttcaacgt ctgccgcggg cacagcgggt ggggcttgcc gtcgtgggtg
      841 acgaagttgc caaggaagaa cggcacgccg ccatcccacg gcacggtgcg ataggtgccg
      901 aggtcgattc gcgccagggc atcgggaaag ccgtgctgcc atccggtgag cttggtgttg
      961 tcgtagcact ggtcacccga gtcccagccg aacaccacgt cgcagaagcc gaagccgccc
     1021 tcgacggcgg agaagaactt gtcgcgatgg atgtacttgc cgcgcaggat cccgtcgatg
     1081 tccgagaccg cgaccttcac cttgccgatc gtcgacttgc gcagggcctc gacgatgttg
     1141 gcggcgtcgc gggcgtcgtt cttcgttttc ttcatgagcc tttctccgtg cagcgtcccg
     1201 cgatctcgag gaagtgggcg agcgaggcgg tctgcagccc ggcgactttt gcggcgtcat
     1261 cccagcggcg cacgcgcatc gcgtcttcgg cgaagggctt ggcgaggaag ccgtcggtct
     1321 cctcgggcga gaagatgccg ccctgcagcg tgaggctgcg cttggaatcc tcggaaagcg
     1381 cctcgtagta atcgggatcg atcgcgcaga gcgcgcgctt cgcgtcgacg tgcaggcgga
     1441 tcgcctcgac gatggcgggc gggaagagcg cccggatgaa cgggatcgcg acgtactggt
     1501 gctggtcgtc gatgccgcgc gccgtgggcg tgtcgccctg ctggttcagc aagtggccca
     1561 ggtcgtggag cagcgaagcg gcgaccaacg cgtcggccgc gccggcctgc tccgcgagct
     1621 gggcggcctg gagcgcgtgc tcgagctgcg tcacgccttc gccgctgtag gcgatgtgcc
     1681 catggtcgcg aaagaccttc tcgatgtctc cgagcttgag ggacatggct acttcgtccg
     1741 tcccgcgagc tcgttggcgg gaggcgcgaa gtccggccgc gcctggtaga tgccggtggc
     1801 gcccatgagg cggctcttgc gaagctgccc ggcctgctcg aggatcgggt aggcggtcga
     1861 gcagaagaag gagttgatgc gccgcatgtc gctgatgagg tcgaggtgca gcgagctcgt
     1921 ctcgatcgat tgcagcgtct gcccggcgag ccggtcgagg tgcgagtcgt ggtaggcgcg
     1981 ctccaggtcg cggaagcgct ccttctcgac gaggagctgc tgcgcgctct tcacgtcgcc
     2041 gttgaggaag accgaaagcc ccaggcgcag gttcaccacg agcttctcgt ggagctccgt
     2101 gatctcgcgc attcccgcct cggagaacga caggcgctgg gcgatcttct tgtcgcgcag
     2161 gtcctggagc atccgttcga ggatatcgcc gacgtgctcg aggttgatgg tgagcgagat
     2221 gatctcggtc cagcgccggc cgtcgcgttc gtcgagcgcc tcgcggctca cctgcgtgag
     2281 gtagagcttc accgccgtgt agaggcgatc cacgtcgtcg tccagcttga tgattgcgtc
     2341 gacgcgcgag acgtcgttgg tgcggatcac ctcgagcacg ccgttcagca tctgctcgat
     2401 ggtgtcgccg atgcgaagcg tctcgcgcgc ggcgttggcc agcgcgaggg cgggcgtccc
     2461 gagggcggcg gcgtcgagga agcgcggcgc ggtctgccgg cgctccgtcg attcgggcag
     2521 ccatttctgg acgaggcccg cgacgcggtc ggtgaagccg atgaacacca cggcgagcgt
     2581 caggttgaag agcacgtgga aatccagaac ctggcggcgc gcgtcggggt cgaaccgcgc
     2641 gatccactcg agcgcccagg gaagcgccag cgagaaggcg ccgcatccgg cgaccttgaa
     2701 gaggaggttg ccgaaggcga cgcgccgccc cgcgccgggc gtgcgggcat tcacgatgag
     2761 cccgaggatg ccgctgccga ggttcgcgcc gagcaccagg cagagcgcga cgggaacgga
     2821 gaccaccttg gtggccgcga gcgtcgcgac gatgagcacc acggcgaggc tcgaccacga
     2881 cagcaccgcg aacgcggcgc cgatcagcat gtcgagcatc gtatcccccg agagggtcgc
     2941 gaagatcacg cgcaccccct gggcctcggt gatgggccgg gtcgcgagca tcacgagctg
     3001 cagcgcgagg agcatgaggc ccaggccgat ggcgacccgg cccatctggc cggtgcgcga
     3061 gttgcgcttc gacaggaaga aggtcacgcc gaggatgatg agaagcggcg aaagccacga
     3121 caggtcgagg gagaagacct gcgacatgag cgccgtcccg acatcggcgc cgagcatcac
     3181 ggcgagcgcc gccgacagcc cgatgaggtt ttgcgagacg aaggagctcg cgatgagcgc
     3241 cgtcgcgctc gacgattgca cgacgccggt cacggcgaga cccgcgagaa aggcggagcc
     3301 gcggttcgag gtgctcttcg agaggagacg tcgcaggtcg gcgccgaaga tgcgcaacac
     3361 cccgctcctc acgatgtgag tgccccacac gaggagcgcg acgccggcga ggaggttc
//
LOCUS       DASVII010000586         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_135255,
            whole genome shotgun sequence.
ACCESSION   DASVII010000586 DASVII010000000
VERSION     DASVII010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135255"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VF309_07630"
     CDS             <1..659
                     /locus_tag="VF309_07630"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX7466494.1"
                     /translation="HDLGAFSMMSSDSQAMGRVGEVVIRTWQTAHKMKVQRGSLKGDP
                     KTHDNARVKRYVAKFTINPAIAHGISHAVGSIEVGKIADLVVWRPAFFGVKPSLIVKG
                     GMIAAAAMGDPNASIPTPQPVHYRPMFGAFGNALQTSVTFVSRSALDNPQVRGLGLRK
                     PLEAVRATRRIGKKDMVHNSLQPKLEVDAETYEVRADGELLTCEPAKTLPMTQRYFLF
                     "
     gene            665..1168
                     /gene="ureE"
                     /locus_tag="VF309_07635"
     CDS             665..1168
                     /gene="ureE"
                     /locus_tag="VF309_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX7466495.1"
                     /translation="MIEIRSRFPLRPSPAYGVQVRGQLSLPFERRQKSRQLASLVSGE
                     TVAIVLPRGAVMRGGDWVVASDGRVIEVVAQTEQVLHVVCETPEALARAAYHLGNRHV
                     PVQVGAGWLRLLADHVLHGMLEGLGAKVTTLEAPFEPEAGAYGAHHRHDNESGHGGKI
                     HEFGETP"
     gene            1165..>1650
                     /locus_tag="VF309_07640"
     CDS             1165..>1650
                     /locus_tag="VF309_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX7466496.1"
                     /translation="MNVKLLQLASPALPVGAYSYSQGLEAAVEAGIIRDAATAGRWIG
                     DVLALSVLGMEAPLLLRLVGAWESSDEVAVRRWNDELVASRETAELRAETLQMGYSLR
                     RLVSELGVEGAPRLDAFEELAYPTAFAFAVAAWRIDAREALAAYLFAWIENQVLAAVK
                     TV"
BASE COUNT          253 a          569 c          579 g          249 t
ORIGIN      
        1 tccatgacct cggcgccttc tcgatgatgt cgtcggactc ccaggcgatg ggccgcgtgg
       61 gggaagtcgt gatccgcacc tggcagacgg cgcacaagat gaaggtgcag cgcggcagcc
      121 tcaagggcga tccgaagacc cacgacaacg cgcgggtgaa gcgatacgtc gccaaattca
      181 ccatcaaccc cgccatcgcc cacggcatct cccatgcggt gggatccatc gaggtgggca
      241 agatcgccga cctcgttgtg tggaggccgg ccttcttcgg cgtgaagccc tcgctcatcg
      301 tgaagggcgg gatgatcgcc gccgccgcga tgggggaccc gaacgcgtcg atccccacgc
      361 cccagccggt gcactaccgg ccgatgttcg gcgcgttcgg caatgccctg cagacctctg
      421 tcacattcgt gtcgcgctcc gcgctcgata atccgcaggt gcgcggcctg ggcctgcgca
      481 agcccctcga ggcggtccgc gcgacgcgcc gtatcggcaa gaaggacatg gtgcacaact
      541 cgctgcaacc gaagctcgag gtcgatgccg aaacctacga ggtgcgcgcc gacggcgagc
      601 tcctcacctg cgagccggcg aagaccctcc cgatgacgca gcgctatttt ctcttctagc
      661 ggccatgatc gagatccgct cgcgtttccc gctgcgcccg agccccgcct acggcgtgca
      721 ggttcgcggg cagctgagcc ttcccttcga gcggcgccag aagagccggc agctcgcgag
      781 cctcgtctcg ggcgagacgg tggcgatcgt gttgccgcgc ggcgccgtca tgcgcggcgg
      841 cgattgggtg gtcgcatccg acgggcgcgt gatcgaagtg gtcgcgcaga ccgagcaggt
      901 gctgcacgtc gtgtgcgaga cgcccgaggc cctggcgcgg gcggcgtatc acctcggcaa
      961 ccgccatgtg ccggtccagg tcggcgcggg ttggctgcgg ctcctggccg atcacgtgct
     1021 gcacgggatg ctcgaaggcc tcggcgcgaa ggtgaccacg ctcgaggcgc ccttcgagcc
     1081 cgaggccggc gcctacggcg cgcaccatcg gcacgacaac gagtcgggcc acggcgggaa
     1141 gatccacgaa ttcggcgaga cgccatgaac gtgaagctgc tgcagctcgc gagcccggcg
     1201 cttcccgtcg gcgcctacag ctactcgcag ggcctcgagg ccgccgtcga ggcgggaatc
     1261 atccgcgacg ccgccaccgc cgggcgatgg atcggcgacg tgctcgcgct ctcggtcctc
     1321 ggaatggagg cgccgctcct gctgcggctc gtcggcgcgt gggaatcgag cgacgaggtc
     1381 gccgtgcgcc gctggaacga cgagctcgtc gcgagccgcg agaccgcgga gcttcgcgcg
     1441 gagacgctgc agatggggta ctcgctccgc cgcctggtga gcgagctcgg cgtcgagggc
     1501 gcgccgcggc tcgacgcgtt cgaggagctc gcgtacccga ccgcattcgc gttcgccgtc
     1561 gcggcgtggc gcatcgacgc ccgtgaagcg ctcgcggcgt atctctttgc atggatcgag
     1621 aaccaggtcc tcgccgcggt gaagaccgtg
//
LOCUS       DASVII010000587         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_27744, whole
            genome shotgun sequence.
ACCESSION   DASVII010000587 DASVII010000000
VERSION     DASVII010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27744"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VF309_07645"
     CDS             <1..1238
                     /locus_tag="VF309_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7466497.1"
                     /translation="MPIIEQAEREGKLKPGGTIVEGTSGNTGVGLAIAAALKGYKCIF
                     TMPDKMSQEKVRLLKAFGAEVIITPTAVAHDHPDNYVMMAKRIAESTPNSILANQFYN
                     QANPDAHYATTGPELWEQSEGRITHFVAAAGTGGTITGVARYLKEKNPHIKIIAGDPQ
                     GSILAELWKSKGKSKPEGAPYKVEGIGQDKLPGTLDLSLVDDYRTVSDRDSFAMARRL
                     TREEGLFVGGSAGLIGHLALQVAREIDDPHAFVVTFLCDTGERYLSKVYNDEWMRENQ
                     LLEPDRVTLGNLLHHKGDGAPEALVSAAPGQLVRQALGLMRLHDVSQLPVLDAGECVG
                     SVTEYALTQRGLESSRFLDAVVGEVMDEPFPVVESTQHVESVQKLLSKATPAVLVRGG
                     GAIQGIVTRSDMLAYLMAR"
BASE COUNT          303 a          449 c          517 g          249 t
ORIGIN      
        1 gcatgccgat cattgaacaa gcagaacgcg aaggcaagct taagcccggc ggaaccatcg
       61 tggaagggac gagcggcaac actggtgtgg ggctcgcgat cgcggccgcg ctcaagggct
      121 acaagtgcat cttcacgatg cccgacaaaa tgtcgcagga gaaggtgcgg ctgctcaagg
      181 cgttcggcgc cgaagtgatc atcacgccca cggcggtggc gcacgaccat ccggacaact
      241 acgtgatgat ggcgaagcgc attgccgagt cgacgccgaa ctcgattctc gcgaatcagt
      301 tctacaacca ggcgaacccc gacgcgcact acgcgaccac cggaccggag ctctgggagc
      361 agtccgaagg gcgaatcacc cacttcgtgg ccgcggcggg aacgggcgga acgatcaccg
      421 gcgtcgcgcg ctacctgaag gagaagaacc cgcacatcaa gatcattgcc ggtgatccac
      481 aggggtccat tctcgcggag ctgtggaaga gcaagggaaa gagcaagccg gagggagcgc
      541 cgtacaaggt ggagggcatt ggacaggaca agctgccggg cacgctcgat ctctcgctcg
      601 tagacgacta tcgcacggtg agcgaccgcg actcctttgc catggcgcgg cgcctcacgc
      661 gcgaggaggg gttgttcgtg ggagggtcgg cggggctgat cgggcatctt gcgttgcagg
      721 tggcgcgcga gatcgacgat ccgcatgcgt tcgtggtgac cttcctctgc gacaccggcg
      781 agcgctacct ctcgaaggtg tacaacgacg agtggatgcg agagaatcag ctgctggagc
      841 cggaccgcgt caccctgggc aacctgctgc accacaaggg cgacggtgcc cccgaggcac
      901 tcgtgagcgc ggctccgggc cagctcgtgc ggcaggcgtt ggggctcatg cgactgcacg
      961 acgtctcgca gcttccggtg ctcgatgccg gagaatgcgt ggggagcgtg acggaatatg
     1021 cactcaccca acgtggcctg gagagctccc gctttctcga cgcggtggtc ggcgaggtga
     1081 tggacgagcc ctttccggtg gtggaatcca cgcaacacgt ggaatcggtc caaaagctgc
     1141 tctcgaaagc gacgccggcg gtgctggtgc gtggcggcgg cgcgattcag gggatcgtga
     1201 cgcgaagcga catgctggcg tacttgatgg cgcgctgact gatagctggc aacgtggccg
     1261 gcaaactgat gaccgccgac tgggtctact agtcggctag tctgctcgtc gcattcaagc
     1321 cactagcagc cggcccagga ctagcagact agcagactag cagactagcc gaccggtctt
     1381 aaggttcact ttcactcgcg agtcacagcg tgcgcatcac agttctcatg ggcggcacct
     1441 catccgagcg cgatgtgtcg ctcgcctccg ggcttcgtat tgccgaggcg ttgcgaacgc
     1501 ggggacatac ggttgcgt
//
LOCUS       DASVII010000588         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_602539,
            whole genome shotgun sequence.
ACCESSION   DASVII010000588 DASVII010000000
VERSION     DASVII010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602539"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1364
                     /gene="gcvT"
                     /locus_tag="VF309_07650"
     CDS             258..1364
                     /gene="gcvT"
                     /locus_tag="VF309_07650"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7466498.1"
                     /translation="MVDATPLKRTPLYDVHVALGAKIVPFAGFEMPVQYPTGITAEHK
                     AVREKAGLFDVSHMGEFLVRGPQAIDFVNYVTTNDVKSLAVGQAQYSTILREDGTIVD
                     DCLVYRSEDRVLMVVNGSNIDKDFAHISKYVGKFDAKLEDISDTIALLALQGPDAARI
                     LQKLTVTDLSKIKYYEFITGKVAGVDKVYISRTGYTGEDGFELYFPGEHAKTIWNALT
                     ASGEVTPTGLGARDSLRLEMGMALYGNDLDDTTTPLEASLGWLVKMKKGDFVGRDALA
                     KQKEQGLGRKLVGFTTAERSFPRHGYPVFANGKASGEVRSGTMSPTLGIPIGTAYVPP
                     GSAAEGSPLEIEIRGKRIPATVQKMPFYKDGSRL"
     gene            1361..1858
                     /locus_tag="VF309_07655"
     CDS             1361..1858
                     /locus_tag="VF309_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX7466499.1"
                     /translation="MIRVAAITVSDSVVRGARQDTSGAVIAGWVKAKKYELVSAAACA
                     DETVEIVRALLRACDTEGADLVLTTGGTGLSARDVTPEATRSVIEREAQGIAERIRIM
                     AMPTFPRAALGRGIAGVRGKSLIVNLPGSPSGVRDGLDALDPIIQHACDILSGKVTEH
                     TLVAT"
BASE COUNT          379 a          578 c          614 g          325 t
ORIGIN      
        1 ccatcatcat ggtcgaagcc gagatggcta cctacatgaa ggtgagcaag tcccggccga
       61 tcatcctgtt cgatttcctc aagtcgaagg tgaccgggtc acggcccacc ctgggcgagg
      121 tcaaggagat caccggggcc gaattgatgg attaggcaga gctttccggg tttttctgcc
      181 aaattgtcac ggcgcccctc gaattcgagg ggcgcttcgc gcatattggg cgagacctca
      241 ggcaaggccg tccagcaatg gttgacgcga cacccctcaa gcgcacgccc ctctacgacg
      301 ttcacgtagc cctgggcgcc aagatcgtcc ccttcgccgg gttcgagatg ccggtgcagt
      361 acccgacggg gataacagcc gagcacaagg ccgtgcgcga gaaggcggga ttgttcgacg
      421 tgagccacat gggcgagttc ctggtgcgcg ggccacaggc gatcgacttc gtgaattacg
      481 tcaccacgaa cgatgtcaaa agcctcgcgg tcggccaggc ccagtactcg acgattctcc
      541 gcgaagatgg aacgatcgtc gacgactgtc tcgtctatcg ctccgaagat cgcgtgctga
      601 tggtcgtgaa cggatcgaac atcgacaagg acttcgcgca catctcgaag tacgtcggga
      661 agttcgacgc caagctcgag gatatcagcg acacgatcgc gctcctcgcg cttcaggggc
      721 ccgacgccgc gagaatcctc cagaagctca ctgtcaccga tctctcgaag atcaagtact
      781 acgagttcat taccggcaag gtcgccggcg tcgataaggt ctacatctcc cgcacaggct
      841 acacgggtga ggatgggttc gagctttact tcccgggcga gcacgcgaag acaatctgga
      901 acgccctcac cgcctccggc gaagtcaccc ccaccggact cggcgcgcgc gactcgctgc
      961 ggctcgagat gggaatggcg ctctacggca acgacctcga cgacactacc acgccgctcg
     1021 aagcatcgct cggctggctg gtgaaaatga agaagggcga tttcgtcgga cgcgacgcgc
     1081 tggccaagca gaaggagcag ggactcgggc gaaagctggt cgggttcacg accgcggaga
     1141 gatcctttcc gcgacacggt tatccggtgt tcgcgaacgg aaaggcgagc ggcgaggtga
     1201 ggagcgggac gatgagtccg acgctcggca ttccgattgg aaccgcgtac gtgccgcccg
     1261 ggtcggcggc ggaaggatcg ccgctcgaga tcgagatacg tgggaagcgc atcccggcga
     1321 ccgtgcagaa gatgcccttt tataaagacg gcagcagact ttgatccgcg tagcggcgat
     1381 caccgtttcc gattccgttg tgcgcggcgc gcgccaggat acctcgggtg cggtcatcgc
     1441 cggctgggtg aaggcgaaga aatacgagct cgtttccgcg gcggcgtgcg ccgacgaaac
     1501 ggttgagatc gtgcgcgcgc tccttcgcgc gtgcgacaca gagggcgcgg atctcgttct
     1561 caccacaggc ggcaccgggc tttccgctcg cgacgtgacg cccgaggcga cgcgctccgt
     1621 gatcgagcgc gaggcgcagg ggatcgcgga acggatccgg attatggcaa tgccgacatt
     1681 tccccgagcg gcacttggac gcggcatcgc gggagtgcgg ggcaagtctc tgatcgtgaa
     1741 tctgcccggc tcgcccagcg gcgtgcgaga tggtctggac gccctggacc cgatcattca
     1801 gcacgcctgc gacatcctta gcggcaaggt taccgagcac acgctggtag cgacgtgaaa
     1861 gttctgatca ctctcgcgga cgtcgagccc gcggtc
//
LOCUS       DASVII010000589         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_135397,
            whole genome shotgun sequence.
ACCESSION   DASVII010000589 DASVII010000000
VERSION     DASVII010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135397"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VF309_07660"
     CDS             <1..884
                     /locus_tag="VF309_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466500.1"
                     /translation="PVEAYTVELYYNKDLLKKIGVTLPASMQLDQASFGDMVKKSVAA
                     GITPFATGVSDRPYPGAYFLQEMLLRKLGPDDYKLLLTGKLSFKDARVIETMTWVKQL
                     VDAGAYSKSFSTLKLGESHFYFHTNPGALMFPIASWYTGRAFVPQDKGGQPKDFPLGI
                     MQYPVPNGAKCPECKTLAVGGSYVMYSRGKNKECAGRMLNSMATVDNGNKWMQAVLLQ
                     TGVRTDPSKIQSIYAPYFRELQERNAKAKYFIGTPLQYMGGKCADAFTQVLNRAFPGG
                     QMGVEDAAAQMDAACKA"
     gene            889..>1609
                     /locus_tag="VF309_07665"
     CDS             889..>1609
                     /locus_tag="VF309_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7466501.1"
                     /translation="MSGVDTRTARWPALMVFLLPALAVYAAFTAYPVFRTFYNSFFDI
                     ADLDTARYVGLEHFRALAGDSTFRAAVRNTMIWSFVAPIFDVGTGLLLALCLYAGVPF
                     ARFFRVAWFTPVLLSYVVVGIMWLWIYNYDWGAVNAMLRAAGLPGLAHSWLGDSNTAL
                     AALMVTHAWKWAGFNMVVCLAAMHSLPSEVLEAAELDNCGWRAKMVHIVIPLLWPTLL
                     NLYILAFIGKMKVFDLVFIMTD"
BASE COUNT          272 a          528 c          514 g          295 t
ORIGIN      
        1 tgccggtcga ggcctatacg gtcgagctct actacaacaa ggacctgctg aagaagatcg
       61 gggtcacgct gccggcgagc atgcagctcg accaggcgtc gttcggcgac atggtgaaga
      121 aatcggtcgc ggcgggcatc acgccgttcg caacgggtgt gagcgaccgg ccctatcccg
      181 gcgcctactt cctccaggag atgctgctgc gcaagctcgg gcccgacgac tacaagctgc
      241 tgctcaccgg caagctctcg ttcaaggacg cgcgcgtgat cgagaccatg acgtgggtca
      301 agcaactggt cgacgccggg gcctattcga agagcttctc cactctgaag ctcggcgaat
      361 ctcatttcta cttccacacc aaccccggcg cgctcatgtt cccgatcgcc agctggtaca
      421 ccgggcgcgc gttcgtgccg caggataagg ggggccagcc gaaggacttc ccgctcggca
      481 tcatgcagta cccggtgccc aacggcgcga agtgccccga gtgcaagacg ctcgctgtcg
      541 gtggcagcta cgtgatgtac tcgcgcggca agaacaagga gtgcgcgggg cgcatgctca
      601 attccatggc caccgtggac aacggcaaca agtggatgca ggcggtgttg ctgcagaccg
      661 gggtgcgcac cgatccttcg aagatccaga gcatctacgc gccctatttc cgcgagcttc
      721 aggagcgcaa cgccaaggcg aagtacttca tcggcacgcc gctgcaatac atgggcggaa
      781 aatgcgccga cgccttcacg caggtgctca accgcgcgtt cccgggcggg cagatgggcg
      841 tggaggacgc cgccgcccag atggatgcag cctgcaaggc gtaggacctt gagcggggtc
      901 gacacccgca cggcgcgctg gccggcgctg atggtgttcc tgttgccggc gctcgcggtg
      961 tacgcggcgt tcaccgcgta cccggtcttc aggaccttct acaacagctt cttcgacatc
     1021 gccgacctcg acaccgcgcg ctacgtggga ctcgagcatt tccgcgcgct cgccggcgac
     1081 agcactttcc gcgccgcggt gcgcaacacg atgatctggt cgttcgtggc gccgatcttc
     1141 gacgtgggca cggggctgct cctcgcgctc tgcctctacg cgggcgtgcc cttcgcgcgc
     1201 ttcttccgcg tcgcgtggtt cacgccggtg ctgctctcct acgtcgtggt cggaatcatg
     1261 tggctgtgga tctacaacta cgactggggc gcggtgaatg cgatgctgcg cgccgcgggc
     1321 ctgcccggcc tcgcgcattc gtggctcggg gactccaaca ccgcgctcgc cgcgttgatg
     1381 gtcacccacg cctggaagtg ggcgggcttc aacatggtgg tttgcctcgc ggcgatgcat
     1441 tcgctgccct cggaagtgct cgaggcggcc gagctcgata actgcggatg gcgcgccaag
     1501 atggtccaca tcgtgatccc gctcctgtgg ccgacgctgc tcaacctcta catcctcgcc
     1561 ttcatcggca agatgaaggt gttcgacctg gtattcatca tgaccgacg
//
LOCUS       DASVII010000590         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_567020,
            whole genome shotgun sequence.
ACCESSION   DASVII010000590 DASVII010000000
VERSION     DASVII010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567020"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VF309_07670"
     CDS             <1..269
                     /locus_tag="VF309_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7466502.1"
                     /translation="SAFRGWNDIWLDPAFRDWNIERDIEAVRCPVLALQGLDDEYGTM
                     EQIHRIARRVPGARLLELERCGHSPHRDQPGRVIAAAVEFAGSR"
     gene            309..1472
                     /locus_tag="VF309_07675"
     CDS             309..1472
                     /locus_tag="VF309_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466503.1"
                     /translation="MKRSTLAAALAALAVAGVAPAAQAQQKLKVGLMLPYSGTYAALG
                     AAIENGFKLYVAEQGGKIGGREIEYFKVDDESEPAKATDNVGKLIKRDNVDVIVGTVH
                     SGVAMAMAKAAKDSGTLLIIPNAGAVAVTGPMCAENIFRSSFSNWQPGYAMGEVAAKK
                     GMKKAVTITWKYAAGDESVGGFKEAFEKGGGQVVKELSLPFPNVEFQPLLTEIASIRP
                     DVVYTFFAGGGAVKFVKDYAAAGLKKTIPLYGAGFITDGTLEAQGADADGLLTTLHYG
                     DGLDNPNDKAFRASYEKSYKVAPDVYAVQGYDAAQMLAIGLNATHGDVAKKAEFAKAV
                     TAARIDSPRGAFTIGASHNPVQDMYLRQVIGKENKVIGVAVKALADPGTGCRM"
BASE COUNT          285 a          498 c          517 g          208 t
ORIGIN      
        1 attccgcgtt tcgcggctgg aacgacatct ggctcgaccc ggccttccgc gactggaaca
       61 tcgagcgcga catcgaggcc gtccgctgcc cggtgctcgc gctgcagggc ctcgacgacg
      121 agtacggcac gatggagcag atccatcgca tcgcgaggcg cgtgcccggg gcgcgcctcc
      181 tcgagctcga gcgttgcggg cactccccgc accgcgacca gcccgggcgc gtgatcgccg
      241 cggcggtgga gttcgccgga tcgcgttaac attccccgct gaaaccaaaa tacagaagga
      301 ggagcaccat gaagagatcg actctcgcgg ccgcgctcgc cgcgctcgcc gtggcgggcg
      361 tcgcgcccgc ggctcaagcg cagcagaagc tgaaggtcgg cctcatgctt ccctactcgg
      421 ggacctacgc cgccctcggc gcggcgatcg agaacggctt caagctctac gtcgccgagc
      481 agggcggaaa gatcggcggc cgcgagatcg agtacttcaa ggtcgacgac gagagcgagc
      541 ccgcgaaggc gacggacaac gtgggcaagc tcatcaagcg cgacaacgtc gacgtgatcg
      601 tcggcaccgt gcattcggga gtcgcgatgg cgatggcgaa ggccgcgaag gacagtggca
      661 cgctcctcat cattccgaac gcgggcgcgg tcgcggtgac cggccccatg tgcgccgaga
      721 acatcttccg cagctccttc tccaactggc agccgggcta cgcgatgggc gaagtcgcgg
      781 cgaagaaggg catgaagaag gcggtcacca tcacctggaa gtacgcggcg ggcgacgaat
      841 ccgtcggcgg cttcaaggaa gcgttcgaga agggcggagg acaggtcgtg aaggagctga
      901 gcctgccgtt ccccaacgtg gaattccagc cgctgctcac cgagatcgcg tcgatccgtc
      961 ccgacgtcgt gtacaccttc ttcgcgggtg gcggggccgt gaagttcgtg aaggactacg
     1021 ccgccgcggg actgaagaag acgatcccgc tctacggcgc gggcttcatc accgacggca
     1081 cgctcgaggc gcagggcgcc gatgccgacg gcctcctcac gacgctccac tatggcgacg
     1141 gcctcgacaa cccgaatgac aaggccttcc gcgcctcgta cgagaagtcg tacaaggtgg
     1201 cgcccgacgt ctacgcggtg cagggctacg acgcggcgca gatgctcgcg atcggattga
     1261 acgcgaccca cggcgacgtg gcgaagaagg ccgagttcgc gaaggccgtc accgccgcga
     1321 ggatcgacag cccgcgcggc gccttcacca tcggcgcctc ccacaatccg gtgcaggaca
     1381 tgtacctgcg ccaggtgatc ggcaaggaga acaaggtgat cggggtcgcc gtgaaggcgc
     1441 tcgccgatcc cggaaccgga tgccgcatgt gaggagcggg ggccgcgccg cactcccatg
     1501 gacgtagc
//
LOCUS       DASVII010000591         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_135509,
            whole genome shotgun sequence.
ACCESSION   DASVII010000591 DASVII010000000
VERSION     DASVII010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135509"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VF309_07680"
     CDS             complement(<1..411)
                     /locus_tag="VF309_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466504.1"
                     /translation="MNTSRCRTLALWALAATTAALSAATLTVPALGAATPGSSTPSTS
                     LRSLLSSHELWATIDVCDTPHHRNTVGVRGSMPGDGNTHDKLYMSFRLQYLNNANSLW
                     VNLPASTAPAFVPVGAGTTARQGGTSFQLVPVAGR"
     gene            517..1191
                     /locus_tag="VF309_07685"
     CDS             517..1191
                     /locus_tag="VF309_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7466505.1"
                     /translation="MVELLGRVPAFSTLEQGDLERIAEVAVPRSFEPGQVVFREGDSS
                     DTCYVVREGHARATRTHGDGRTITLATFGPGDIFGELAMFEDELRSATVEAIEPTSVV
                     GVLGGDMRRLMGEHPGISMRLVIALGRRLRETNERLSRQSFQTVQSRVAVVLSQLVEQ
                     EIADGRGPADVLVTATQVDLAQLAGSSRESASRFLAVLERAGVISQGRGRLTVHDLQA
                     LKQYVF"
     gene            1181..1654
                     /locus_tag="VF309_07690"
     CDS             1181..1654
                     /locus_tag="VF309_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7466506.1"
                     /translation="MSSRSAKPARKRGRDKGKGREFSAGGVVVRGEEVVTIVPTRRAA
                     DGSRVLALPKGHVDPGESPIEAAAREVREETGLVAEPVCELGESRYWYRRDGRTIGKT
                     VSFFLFNHVEGSIEDHDDEVEEVRWMGLREAETALSHTAEREMIALALAHLAKDR"
     gene            1668..2066
                     /locus_tag="VF309_07695"
     CDS             1668..2066
                     /locus_tag="VF309_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX7466507.1"
                     /translation="MQVLNFYSTIFADELKRGRKTATIRLGDKSSKYRKNQAVLVTIG
                     YQYSPRERIFEAVIDQVEVMKLADLSPRDIKHDNPEFRRHEELINFLEQIYGREVSPE
                     DTVTVVRFSQILDTPSGMTEAIKGFGGAQN"
     gene            2168..>2366
                     /locus_tag="VF309_07700"
     CDS             2168..>2366
                     /locus_tag="VF309_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7466508.1"
                     /translation="MKLKPLGDRLIVQAIEEEQTTASGIVLPDTAKEKPQKGKVLAAG
                     DGRLDEAGKRIPMDVKEGDEVL"
BASE COUNT          400 a          788 c          817 g          361 t
ORIGIN      
        1 ccggcccgcg accggcacca gctggaagct cgttccgccc tggcgcgcgg tcgtgcccgc
       61 gccgaccggc acaaacgctg gggcggtgct tgccggcagg ttcacccaca agctgtttgc
      121 gttgttgagg tactgcaggc gaaagctcat gtagagcttg tcgtgcgtgt ttccatcacc
      181 cggcatcgag ccgcgcacgc cgaccgtgtt gcggtggtgc ggggtgtcgc acacgtcgat
      241 cgtggcccac agttcgtgag aggagagcag cgagcgcagg ctcgtgctcg gtgtgctgga
      301 gcccggcgtg gccgcgccca gcgcgggaac ggtgagcgtc gccgcgctta gtgccgcggt
      361 cgtcgcggcg agtgcccaga gggcgagcgt gcgacacctc gatgtgttca tgctggtgag
      421 attagcgccg gcgatcccct ccgtgtgcag gcaagcggcg ccgcggtcgg cggttgtggt
      481 atcccgtggg cgatgggggc gactcgctca gctgaggtgg tcgagctgct cggtcgcgtg
      541 cccgcgttct ccacgctcga gcagggcgac ctcgagcgga tcgccgaggt ggcggtgccg
      601 cgcagcttcg agcccgggca ggtggtcttc cgcgagggcg actccagtga cacctgctac
      661 gtggtgcgcg agggtcacgc ccgcgccacc cgcacccacg gggacgggcg cacgatcacg
      721 ctcgcgacgt tcgggccggg cgacatcttc ggcgagctcg cgatgttcga ggacgagttg
      781 cgctccgcca ccgtcgaggc gatcgagccg acgagcgtcg tgggcgtgct cggcggcgac
      841 atgcgcaggc tgatgggcga acacccgggg atctccatgc gcctcgtgat cgcgctcggc
      901 aggcgcttgc gtgagaccaa cgagcgactc tcgcgccagt ctttccagac ggtgcagagc
      961 cgcgtcgcgg tcgtcctcag ccaactcgta gagcaggaga tagctgacgg caggggaccc
     1021 gccgacgtgc tcgtcaccgc cacccaggtc gacctcgcgc agctcgccgg ctcctcacgc
     1081 gagtccgcca gccgcttcct cgcggtgctc gagcgcgccg gggtgatctc ccagggccgt
     1141 ggccgtctga ccgtccacga cctgcaggcc ttgaagcagt atgtcttcta ggtccgccaa
     1201 gcccgcgcgc aaacgcgggc gggacaaggg caaggggcgc gagttctccg ccggcggcgt
     1261 ggtcgtgcgg ggcgaggagg tcgtcacgat cgtgcccacc cgtcgtgccg ccgacggctc
     1321 gcgcgtgctc gcgctgccca agggccacgt ggaccccggc gagagcccga tcgaggcggc
     1381 ggcgcgcgag gtgcgcgagg agaccgggct cgtggccgag cccgtgtgcg agctgggcga
     1441 atcgcggtac tggtacaggc gcgacgggcg cacgatcggc aagaccgttt ccttcttcct
     1501 cttcaaccac gtcgaaggca gcatcgagga ccacgacgac gaggtcgagg aggtgcgctg
     1561 gatgggcctg cgcgaggccg agaccgcgct cagccatacc gccgagcgtg agatgatcgc
     1621 gctcgcgctc gcccacctgg ccaaagaccg atagcctgct caccgcgatg caggttctca
     1681 acttctactc cacgatcttc gccgatgagc tcaagcgcgg acgcaagacc gccacgatcc
     1741 gcctcggcga caagtccagc aagtaccgca agaaccaggc tgtgctggtc acgatcggct
     1801 accagtactc gccgcgcgaa cggatcttcg aagccgtcat cgatcaggtc gaggtgatga
     1861 agctcgcgga cctctctcca cgggacatca agcacgacaa ccccgagttc cgccgccacg
     1921 aggagctgat caacttcctc gaacagatct acggccgcga ggtctccccc gaagacaccg
     1981 tgaccgtcgt gcgcttctcg cagatcctcg acaccccatc aggcatgacc gaggccataa
     2041 agggcttcgg aggcgcacaa aactagctat attgagaagc gttggcactc tccccccgag
     2101 agtgccaaaa gaggtggtcc tccgcgggcc gccggtcgca tttctcaatg atggaggtcg
     2161 catacacatg aagctcaagc ccctgggcga tcgtctgatc gtgcaggcga tcgaggagga
     2221 gcagaccacc gcgagtggca tcgtcctccc cgacaccgcc aaggagaagc cgcagaaggg
     2281 caaggtcctc gccgcaggcg acggacggct cgacgaggca ggaaagcgca tccccatgga
     2341 tgtgaaggag ggcgacgagg tcctct
//
LOCUS       DASVII010000592         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_98791, whole
            genome shotgun sequence.
ACCESSION   DASVII010000592 DASVII010000000
VERSION     DASVII010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98791"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VF309_07705"
     CDS             <1..1047
                     /locus_tag="VF309_07705"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7466509.1"
                     /translation="RAFQRLRYVRQLGLAYLVYPGASHSRFEHALGAYHLARRALAIF
                     DERDSGPALASGDAVLARCAALLHDVGHYPFSHALEEIGALHHEEVARPLITTGEIAE
                     VLRDGLGGDAPDRIMALIRGGSSSPLQGLISGSLDLDKIEYLKRDAFMCGVNYGDIDV
                     DRLLNSLVIVPDAAGRASRIGISEKGLSALESLLFAKYQMYRNVYWHHAVRSATAMYK
                     RLVDGALRAGSLTAETLAAYTDEGLLHDLESRAPTPLLAALRQRRLYKRIFECPAAEL
                     SADTGEWIADDRALVVAVEDQLAQELGLNPGELLLDYPVKTQMLGLDIPVLRRDGSVR
                     QLTATGWEGAVNLP"
     gene            complement(1248..1508)
                     /locus_tag="VF309_07710"
     CDS             complement(1248..1508)
                     /locus_tag="VF309_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466510.1"
                     /translation="MCHYRNFLSLVFVLPAIALLGRGIDATSQSAAGAGAETKVLICH
                     NAGPSKQIEIEVNESAVAAHIEQHGDTVGACSGVPEDTFNSR"
BASE COUNT          250 a          560 c          534 g          270 t
ORIGIN      
        1 cgcgcattcc aacggctgcg ctacgtgcgg cagcttggat tggcgtacct cgtatatccc
       61 ggcgcgagcc actcacgctt cgagcacgcg ctgggcgcgt atcacctcgc gcgccgcgcc
      121 ctcgccatct tcgacgagcg cgactccggt cctgcgctcg cgtccggcga cgccgtgctc
      181 gcgcgatgcg cggcgctgct ccacgacgtc ggtcactacc cgttctcgca tgcgctcgag
      241 gagatcggtg ccctgcacca cgaggaagtg gcgcgccccc tcatcaccac gggcgagatt
      301 gcggaggtgc tgcgcgacgg gctgggcggt gacgcacccg atcgcatcat ggcgctcatc
      361 cgcggcggga gttcgagccc gctgcaagga ctcatctccg gctcgctcga tctcgacaag
      421 atcgaatatc tcaagcgcga cgcgttcatg tgcggggtga actacggcga catcgacgtc
      481 gaccgcttgc tcaactcgct cgtcatcgtg cccgacgcgg cgggccgcgc gagccgcatc
      541 ggcatctcgg agaagggact ctcggcgctc gagtcgctgc tgttcgccaa gtaccagatg
      601 tatcgcaacg tgtactggca ccacgccgtg cgcagcgcca cggccatgta caaacggctc
      661 gtcgacggcg ccctgcgcgc ggggtcgctc acggccgaga ccctcgcggc gtatacggac
      721 gaagggctgc tgcacgacct cgagtcgcgc gcgccgacgc cgctgcttgc cgcactacgc
      781 cagcggcggc tctacaagcg catcttcgag tgccccgccg ccgagctctc cgccgacaca
      841 ggggaatgga tcgcggacga ccgcgcgctc gtcgtggcgg tggaagacca gctcgcgcag
      901 gagcttggtc tcaaccccgg cgagctcctc ctcgactatc cggtgaagac ccagatgctc
      961 ggcctcgaca ttcccgtgct gcggcgcgac ggcagcgtgc ggcagctcac cgccacgggc
     1021 tgggagggtg cggtcaatct tccgtagctg tccgaggagc tgtacggcag cgcgcggtgg
     1081 ctgcgggtgt tcacctgccg gcgcgtcgac gtggacaaac gcgcgattct ccgcctggcg
     1141 gagatgagcg ggggcgaggt gcgcagccgg ttgggcagca gcttgctcta gcgtccttgc
     1201 tcaccctcag tgccgcgccc tcgtcggcgc gccaccggac cttccgatca gcgggagttg
     1261 aacgtatcct cgggaacgcc actgcaggcg ccaaccgtat caccatgttg ctcgatatgc
     1321 gccgccacgg cgctctcgtt cacttcgatc tcgatctgct tgctcggacc cgcgttgtga
     1381 cagatgagga ccttcgtctc cgctccagcg ccggcggcag actgcgatgt cgcatcgatg
     1441 ccgcgaccca gaagtgcaat cgccggcagt acgaagacca gcgagaggaa attcctgtag
     1501 tggcacatgc gtgactccct gatgtcaggt gggaacgtca ccctatttcc tcgcggcgcg
     1561 cgggccagcg agaggggtgt ggagcgccga gcgcagcgcg atgcgtggct ccgg
//
LOCUS       DASVII010000593         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_567259,
            whole genome shotgun sequence.
ACCESSION   DASVII010000593 DASVII010000000
VERSION     DASVII010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567259"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /gene="argB"
                     /locus_tag="VF309_07715"
     CDS             <1..371
                     /gene="argB"
                     /locus_tag="VF309_07715"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7466511.1"
                     /translation="IGSDENGTAYNINADIAAGKLAITLQAEKLVLLTNTTGVLDKEG
                     KVITGLTAQKVKEMIDDGTISGGMLPKVECALDAVRNGVKSAHIIDGRVSHALLLEVL
                     TSSGVGTLIRGASANRANHR"
     gene            complement(440..1192)
                     /locus_tag="VF309_07720"
     CDS             complement(440..1192)
                     /locus_tag="VF309_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7466512.1"
                     /translation="MSRSKAFNVVTYNIHKGLSQFNKRLVLHEIRDRLKSLDADVAFL
                     QEVQGKHEANARRHVSWPAIGQQDYLTHEGCHNVYGMNAVYQDGHHGNAVVSRYPIPV
                     WENIDVSHHPMESRGLLHCEVQIDGWKDPLHCINVHLGLWARSRRFQLEWLCERIRAS
                     VPKKGPLVVAGDFNDWHRKASNFLEDELGLVEVFEKKEGKPAKSYPAQMPFFTLDRIY
                     VRDLNVETVTRHSGAGWSRLSDHVALAARLSR"
     gene            complement(1195..1638)
                     /gene="nudB"
                     /locus_tag="VF309_07725"
     CDS             complement(1195..1638)
                     /gene="nudB"
                     /locus_tag="VF309_07725"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX7466513.1"
                     /translation="MTHKIPVSTLVVIHAPDLQVLLLERADHPGYWQSVTGGQHEGEA
                     LRDTAIREVGEETGLDAASYRLDAWNLVNVFEISPVWRHRYAPGVTHNTEHVFGLEVP
                     GPLPVRVAPREHLAARWLPWNEAAERVFSWSNRKAILMLPDRKRG"
     gene            complement(1635..>2140)
                     /locus_tag="VF309_07730"
     CDS             complement(1635..>2140)
                     /locus_tag="VF309_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX7466514.1"
                     /translation="ADWQDKRERVGEVLGKHSLKMRRFLQDHPEYAGGTVGAAAVDAD
                     GVLAAATSTGGVTMKLVGRVGDSPIAGAGNYASRYVAASATGTGEYVMRSLAARAVSE
                     KVARGATLDNAMKEVLAELASDYQADVGMIAVDSAGTPVARHLTRDMPHASFAGEGAV
                     VSQMRVA"
BASE COUNT          349 a          723 c          717 g          351 t
ORIGIN      
        1 ccatcggcag cgacgagaac ggcaccgcct acaacatcaa cgccgatatc gccgccggca
       61 agctcgccat cacgctgcag gccgaaaagc tcgtgctcct caccaacacc accggcgtcc
      121 tcgacaagga gggcaaggtg atcacgggcc ttaccgcgca gaaggtgaag gagatgatcg
      181 acgacggcac catctccggc ggcatgctgc cgaaagtcga atgcgcactg gacgcggtga
      241 ggaatggcgt gaagagcgcg cacatcatcg acgggcgcgt gtcgcacgcg ctgctgctgg
      301 aggtgctgac cagctcgggc gttgggactt tgatccgcgg ggcttccgcc aaccgcgcga
      361 accacaggta acttccgcta ccgcgctcaa gacgatccca cggagatcag gagatcgtcc
      421 tgccagcgcc agcgacaggt taacgggaga gtcgcgcggc gagcgccacg tggtccgaga
      481 ggcgcgacca tcccgcgcca gaatgccgcg tcacggtttc cacgttcagg tcgcgcacgt
      541 agatgcggtc cagcgtgaaa aaaggcatct gcgcgggata gctcttcgcc ggcttgcctt
      601 ccttcttctc gaagacttcg acgaggccca gctcgtcctc gaggaaattg ctcgccttgc
      661 ggtgccagtc gttgaagtcc ccggcgacca ccagcgggcc cttcttgggc accgaggcgc
      721 ggatcctctc gcacagccac tcgagctgga agcgccggct gcgcgcccag aggcccaggt
      781 gcacgttgat gcagtgcagc ggatccttcc agccgtcgat ctgcacctcg cagtgcagga
      841 ggccgcggct ttccatggga tggtgcgaga cgtcgatgtt ctcccacacg gggatcgggt
      901 agcggctcac gacggcgttg ccgtggtggc catcctggta gaccgcgttc atgccgtaga
      961 cgttgtggca gccctcgtgg gtgaggtagt cctgctggcc gatcgcaggc cagctcacgt
     1021 ggcggcgcgc gttggcctcg tgcttgccct gcacttcctg caggaacgcc acgtccgcgt
     1081 cgagcgactt caggcgatcg cggatctcgt gcagcaccag gcgcttgttg aactgcgaca
     1141 agcccttgtg gatgttgtac gtgacgacgt tgaaggcctt ggaccgggac attttcagcc
     1201 gcgctttctg tcgggaagca tgaggatcgc ctttcggtta ctccaggaaa acacgcgctc
     1261 ggccgcctcg ttccagggca gccaccgggc ggcgaggtgc tcccgcggcg ccacccgcac
     1321 gggcagcggc ccgggaacct ccaggccgaa gacgtgctcc gtattgtggg tcacaccggg
     1381 cgcgtagcgg tggcgccata caggagagat ttcgaagacg ttcaccaagt tccacgcgtc
     1441 gagccgataa ctcgccgcgt cgaggccggt ttcctcgccc acctcgcgaa tcgccgtgtc
     1501 gcgcagggcc tcgccctcgt gctggccgcc cgtgaccgac tgccagtatc cggggtggtc
     1561 ggcgcgctcg agcagcagca cctggaggtc gggcgcgtgg atcacgacga gcgtcgagac
     1621 cgggatcttg tgcgtcacgc cacgcgcatt tgcgagacca ccgcgccctc gccggcgaag
     1681 gaggcgtgcg gcatgtcgcg cgtgaggtgc cgcgcgaccg gggtgccggc gctatcgacg
     1741 gcgatcatcc cgacatcggc ctggtagtcg ctcgccagct ccgcgaggac ctccttcatc
     1801 gcattgtcga gcgtggcgcc gcgcgcgacc ttctcggaaa ccgcgcgcgc cgcgagcgag
     1861 cgcatcacgt attcgccggt gccggtggcc gaggcggcga cgtagcgcga cgcgtagttg
     1921 cccgcgcccg cgatcggcga gtcgcccacg cgaccgacga gcttcatggt cactcccccg
     1981 gtggaggtgg cggcggcgag gacgccgtcc gcgtccaccg cggcggcgcc gacggtgccg
     2041 cccgcgtatt cgggatgatc ctgcaggaag cggcgcatct tgaggctgtg cttgccgagc
     2101 acctcgccca cgcgctccct cttgtcctgc caatcggcgc
//
LOCUS       DASVII010000594         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_423203,
            whole genome shotgun sequence.
ACCESSION   DASVII010000594 DASVII010000000
VERSION     DASVII010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423203"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1283
                     /locus_tag="VF309_07735"
     CDS             42..1283
                     /locus_tag="VF309_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466515.1"
                     /translation="MNSRRTLPRRIAALALALFTAPLALAQSSAEPIRIGFLTVRTGP
                     LAAGGKQMEEGINLFLKERNNMLAGRKVELIVADTAGAPQQARTKTQELVEKNHVHVI
                     IGPLATFEALAIDDYIKQAQVPLITPTSAAQKDLAQQKQSDYVIHAMGTAAQPMHVLG
                     DFAARKLGLKRMSSIADDFTYGHEGSAGFQAAFEEAGGRIVQKLWPPLNVGDYGSYAS
                     QINADVDGVYAGFAGINGLRFLKQYAEYGLKKPVFGNPTCVDEGILRNMGDEALGVYS
                     ASWYSAELDTPENKRFVDAVMKEYKVVPGFYTSGTYVAAMQLEAALTAIQGRVEDKAA
                     FVKALHEARLEKSPVGPIRIDEYGKPVLNIYVRKVERKDGRLVNSIVATYPAVTQFGN
                     FDPKKFLAAPVYSRDYPSLKN"
BASE COUNT          311 a          541 c          507 g          231 t
ORIGIN      
        1 accgcggcca aactcctcgg catcgcttga agggatcgcc cgtgaattca cgccgcactc
       61 tcccccgtcg catcgcagcg ctcgcactcg cgctcttcac ggcgccgctc gctctcgcgc
      121 aatcgagcgc ggagccgatc cgcatcggct tcctcacggt gcgcaccggc cccctcgccg
      181 ccggcggcaa gcagatggag gaaggcatca acctcttcct caaggagcgc aacaacatgc
      241 tcgccggccg caaggtcgag ctcatcgtcg cggacacggc gggcgcgccg cagcaggcgc
      301 gcaccaagac ccaggagctg gtggagaaga atcacgtcca cgtgatcatc gggcccctgg
      361 cgacattcga ggcgctcgcg atcgacgact acatcaagca ggcgcaggtg ccgctcatca
      421 cgccgacctc ggccgcgcag aaggacctcg cccagcagaa gcagagcgac tacgtgatcc
      481 atgcgatggg caccgcggcg cagccgatgc acgtgctcgg cgatttcgcc gcgcgcaagc
      541 tcggcctgaa gcgcatgtcc tcgatcgcgg atgacttcac ctacgggcac gaaggcagcg
      601 ccggattcca ggcggcgttc gaggaagcgg gcgggcgcat cgtgcagaag ctctggccgc
      661 cgctcaacgt cggcgactac ggctcctacg cgagccagat caacgcggac gtcgacggcg
      721 tgtacgcggg cttcgcgggg atcaacggct tgcgcttcct caagcagtac gcggaatacg
      781 gattgaagaa accggtcttc ggcaatccca cctgcgtgga cgaaggcatc ctgcgcaaca
      841 tgggcgacga agcgctcggc gtgtactcgg cgagctggta ttcggcggag ctcgacacgc
      901 cggagaacaa gcgcttcgtc gacgccgtga tgaaggagta caaggtcgtc ccgggcttct
      961 acacctcggg aacctacgtc gcggcgatgc agctggaggc ggcgctcacc gcgatccagg
     1021 ggcgcgtcga ggacaaggcg gcgttcgtca aggcgctgca cgaagcgcgc ctcgaaaaga
     1081 gccccgtcgg ccccatccgc atcgacgagt atggcaagcc cgtgctgaac atctacgtgc
     1141 gcaaggtcga gcgcaaggac ggccgcctcg tcaattccat cgtcgcgacc tatcccgcgg
     1201 tcacgcagtt cgggaacttc gatccgaaga aattcctcgc cgcgccggtt tattcgcgcg
     1261 actacccgtc gctgaagaac tgagcggccg cggcaccttg gcgaagtggc tgagcatcag
     1321 ccacgacgga aacgcctgcg caagcgctcg ggatccggcc agctgcacct tgcccacgcg
     1381 tagggcgtcc tcgaaggtgc agtctcccag ccagatcctc gccatcgtcg ccaggtcggc
     1441 atcgacgtgg agatcgacct cgaatccggg atcctcgatg cagagatcgg ctttcgtgcg
     1501 atcgagcagg agccagaaga gacgcggacc gcggtaatgg accgccacgc caccgaactt
     1561 gaagcgcacg acaatgcggc gcgtcggcac
//
LOCUS       DASVII010000595         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_207259,
            whole genome shotgun sequence.
ACCESSION   DASVII010000595 DASVII010000000
VERSION     DASVII010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207259"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2337)
                     /gene="treS"
                     /locus_tag="VF309_07740"
     CDS             complement(<1..>2337)
                     /gene="treS"
                     /locus_tag="VF309_07740"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX7466516.1"
                     /translation="VWSDDPAKYAGTRIIFTDTETSNWAWDEVAQQHYWHRFFSHQPD
                     LNYDNPEVLRAVLGTMHFWLDMGVDGFRLDAIPYLIEREGTSNENNPETHAVIKKVRA
                     DLDAAYKGRMLLAEANMWPEDVRQYFGDGDECHMAYHFPMMPRLYMSIAMEDRYPLIE
                     IMQQTPPIPPNCQWAIFLRNHDELTLEMVTDRERDYMYRIFAGDPRMRVNVGIRRRLA
                     PLMENDRARIELITFLLMTMPGAPILYYGDEIGMGDNIYLGDRNGVRTPMQWTPDRNA
                     GFSRADPQRLFLPPVMDAVYGYEAVNVEAQLRSPSSLLQWTRRLIATRRKYKAFGRGT
                     LAFLEPGNRKVLAFLREYDDEAMLCVANLSRVPQAVELDISRFAGRVPVELVGQEPFP
                     PVGKLPYLVTLPGYGFMAFRLSTDAKPPGWHEERLATRRLPVLVLAPGWQETLDRGAP
                     GVDFTQVFMHVSGERLRDEILLPYLKGRRWFAAKNEPIAELRVASIAPWKGESGNTWR
                     IAFVDAKLASGETQRYFVPIALDWETRDHDPLEHYAAFAIAKVRHRERVGIFYAAFAN
                     PDFARDMARAMGSGATAKLGEGSVRFSATGRFPAFAGALEEPAGTPSLEQSNTAVFFG
                     SKLFLKGYRRLRPGVNPELELGRFLTEVSPFANIAPVLGAVEYLEPGAAAPATLAILQ
                     QFIENQGDLWTLTCQHLARALHMPAANPPEGSADSAAAGFHLGRMGLLGRRVAELHRA
                     LCVTSGDARFDPVPVTRADFDGWKDAIERELDATLAAV"
BASE COUNT          361 a          772 c          832 g          372 t
ORIGIN      
        1 cgaccgcggc gagagtcgcg tcgagctcac gctcgatcgc gtccttccag ccatcgaagt
       61 ccgcgcgcgt gaccggcacc ggatcgaagc gcgcatcgcc ggaggtcacg cacagcgcgc
      121 gatgcagctc cgccacgcgc cgcccgagaa gtcccatgcg gcccaggtgg aaccccgcgg
      181 ccgcggagtc cgcgctgccc tcgggcgggt tggccgccgg catgtgcagc gcgcgcgcca
      241 ggtgctggca ggtgagcgtc cacaggtcgc cctggttctc gatgaactgc tgcaggatcg
      301 cgagcgtcgc cggcgccgcg gcgccgggct cgaggtactc caccgcgccc aacaccggcg
      361 cgatgttcgc gaacggcgac acctcggtga ggaaacggcc gagctcgagc tcggggttca
      421 cgccggggcg aagcctccgg taccccttga ggaagagctt cgagccgaag aagaccgcgg
      481 tgttgctctg ctcgagagag ggcgtgcccg cgggctcctc gagcgcgccg gcgaacgcgg
      541 ggaaacggcc ggtggcggag aagcgcacgc tgccttcgcc gagcttcgcc gtcgcccccg
      601 agcccatggc gcgcgccatg tcgcgggcga aatcgggatt ggcgaacgcg gcgtagaaaa
      661 tgccgacgcg ctcgcgatgg cgaaccttcg cgatcgcgaa cgccgcgtag tgctcgagcg
      721 gatcgtggtc gcgcgtctcc cagtcgagcg cgatgggaac gaagtagcgc tgcgtctcgc
      781 cgctggccaa tttcgcgtcg acgaaggcga tgcgccacgt gttgcccgat tcgcccttcc
      841 acggcgcgat cgaggccacg cgcagctcgg cgatcggctc gttcttcgcg gcgaaccagc
      901 gccgtccctt gaggtagggc agcaggatct cgtcgcgcag ccgctccccg gagacgtgca
      961 tgaagacttg cgtgaaatcg acgcccggcg cgccgcggtc gagcgtctcc tgccatccgg
     1021 gcgccagcac caggaccggc aggcgccgcg tggcgaggcg ctcctcgtgc cagccgggcg
     1081 gcttcgcgtc ggtggagagg cgaaacgcca tgaacccgta gccggggagc gtgacgaggt
     1141 agggaagctt gcccaccggc ggaaagggct cctgccccac cagctccacc ggcacgcggc
     1201 cggcgaagcg cgagatgtcg agctcgaccg cctgcggcac gcgcgagagg ttggccacgc
     1261 agagcatcgc ctcgtcgtcg tattcgcgca ggaaagcgag caccttgcgg ttgccgggct
     1321 cgaggaaggc gagcgtgccg cgcccgaacg ccttgtactt gcggcgcgtg gcgatgaggc
     1381 gccgcgtcca ttgcagcagc gacgacgggc ttctcagctg ggcctcgacg ttcaccgcct
     1441 cgtagccgta cacggcgtcc atgacgggcg gcaggaagag gcgctgcggg tcggcgcgcg
     1501 agaagccggc attgcggtcc ggcgtccatt gcatcggcgt cctcacgccg ttcctgtcgc
     1561 ccaggtagat gttgtcgccc atgccgatct cgtcgccgta gtagaggatc ggcgcgcccg
     1621 gcatcgtcat caggaggaac gtgatgagct cgatcctggc gcggtcgttc tccatgaggg
     1681 gggcgagccg ccggcggatg cccacgttca cgcgcatgcg cgggtcgccc gcgaagatgc
     1741 ggtacatgta gtcgcgctcg cggtcggtca ccatctcgag cgtgagctcg tcgtggttcc
     1801 tgaggaagat cgcccattgg cagtttggcg gaatgggcgg cgtctgctgc atgatctcga
     1861 tcagcggata gcggtcctcc atcgcgatcg acatgtagag ccgcggcatc atcgggaagt
     1921 ggtaggccat gtggcactcg tcgccgtcgc cgaagtactg gcgcacgtcc tcgggccaca
     1981 tgttggcttc cgcgagcagc atcctgccct tgtaagcggc gtcgagatcc gcgcgcacct
     2041 tcttgatcac cgcgtgcgtc tcgggattgt tctcgttgct ggtgccctcg cgctcgatga
     2101 gatagggaat cgcgtcgagg cggaatccgt ccacgcccat gtcgagccag aagtgcatgg
     2161 tgccgagcac cgccctcagc acctcggggt tgtcgtagtt caggtccggc tggtggctga
     2221 agaagcgatg ccagtagtgc tgctgcgcga cctcgtccca cgcccagttg gaagtctcgg
     2281 tatcggtgaa gatgatgcgc gtgccggcgt acttggcggg gtcgtcggac cagacgt
//
LOCUS       DASVII010000596         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_531002,
            whole genome shotgun sequence.
ACCESSION   DASVII010000596 DASVII010000000
VERSION     DASVII010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531002"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VF309_07745"
     CDS             complement(<1..727)
                     /locus_tag="VF309_07745"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7466517.1"
                     /translation="MRTPSEQRGFVVPKELHGRPLDGVLKTLASLSWGDARRLIERGK
                     ISVGGQVVTQTDRRLRQGDQIDVRPNAPRPKSTADLDRSALVYVDAQIVVVNKPAGIN
                     TVPFEAGERGTLDQLVAALLPRTRGKGPPSSLGVVQRLDRETSGVLVFARTFAAKKIL
                     AAQLRAHSVHRLYIALAHGAIEKKQTFRSYLVQNRGDRLRGTSRFGREGQLAVTYVDP
                     IRRLAGATLVTCRLETGRTHQIRI"
     gene            complement(711..>1764)
                     /locus_tag="VF309_07750"
     CDS             complement(711..>1764)
                     /locus_tag="VF309_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7466518.1"
                     /translation="ICDLPTLPLVRDGAITAIRENRSTYSFPEGTSTLRRVIADKLGR
                     ENGIHASPETDIVVTVGASGAFTAAIMALLNPGDGLLLFEPYYGYHLNTALLAGIEPQ
                     FATLDLPSFSLDEQRLRRAIRPNTRAIVLCSPSNPSGKMLTRPELESLARIAVEHDLL
                     VITDEIYEYFRFDGREHISPATIAGLEDRTVTIMGLSKTFSITGWRLGYAVARAELAA
                     PISIVNDIYYVCAPTPLQLGAAAGFAAPASYFEELRTSYQIKRDMICDALEKARMSPV
                     VPEGAYYVLADISRSGFSVAKDAAMALLERTKVASIAGSAFFESAIGERYLRFCFAKE
                     DDVLAEACARIRANPL"
BASE COUNT          300 a          514 c          642 g          308 t
ORIGIN      
        1 ggatgcgaat ctggtgcgtg cggccggtct cgagccggca ggtgacgagg gtcgctcccg
       61 cgaggcgccg gatgggatcg acgtaggtga ccgcgagctg tccctcgcgc ccgaagcgcg
      121 atgtcccacg gaggcgatcg cctcgatttt gcacgaggta gctgcgaaag gtttgcttct
      181 tctcgatggc tccgtgagca agagcgatgt agaggcggtg cacgctgtgc gcacgaagct
      241 gcgccgcgag aatcttcttc gcggcgaacg tgcgcgcgaa caccagaaca ccactcgttt
      301 cgcggtcgag ccgctgcacc actccgagcg acgacggagg ccccttgccg cgtgtccgtg
      361 gaaggagcgc agcgacgagc tgatcgagcg tgccgcgctc accagcctcg aagggcacgg
      421 tattgattcc ggccggtttg ttcaccacga cgatctgagc gtcgacgtag acgagcgcgg
      481 agcgatcgag gtcggcggtg ctcttgggtc tcggcgcgtt cggccgcacg tcgatctgat
      541 caccctgacg aaggcgacgg tcggtctggg tgacgacctg gccgcccacc gagatcttgc
      601 ctcgttcgat gagccggcgc gcatctcccc acgagagcga ggcgagcgtc ttgagcacgc
      661 catcgagcgg cctgccgtgg agctccttgg ggacgacgaa gccccgttgc tcagaggggg
      721 ttcgcacgga tcctggcgca agcttcggcg agcacgtcgt cttctttggc gaagcaaaag
      781 cggaggtacc gctcgccgat ggcgctctcg aagaatgccg agccggcgat cgaggcaacc
      841 ttggttcgct cgagcagcgc catcgccgcg tctttggcga ccgagaagcc cgagcgcgag
      901 atgtcggcga gcacgtagta cgctccttct ggcactacgg gcgacattcg cgccttctcg
      961 agcgcgtcgc agatcatgtc gcgcttgatc tggtagctgg tgcgaagctc ttcgaagtac
     1021 gacgctggcg ccgcgaaccc cgcggccgcg ccgagctgca gcggcgtcgg agcgcacaca
     1081 tagtagatgt cgttgacgat ggagatgggg gcggcgagct cggctctcgc cacggcgtat
     1141 ccgaggcgcc agccggtgat gctgaacgtt ttggagagac ccatgatcgt cacggtacgg
     1201 tcctcgagcc cggcgatggt cgctggagag atgtgctcgc gcccgtcgaa gcgaaagtac
     1261 tcgtagatct catcggtgat gacgaggaga tcgtgctcga cggcgatgcg cgccaagctc
     1321 tcgagctccg ggcgtgtgag catcttgccg ctcgggttgc tgggcgagca gagcacgatc
     1381 gcgcgcgtgt tgggtcggat ggcccggcgc aggcgctgct cgtcgagaga gaaagaagga
     1441 agatcgagcg tggcgaactg tggctcgatg ccggcgagga gggcagtgtt gaggtgatag
     1501 ccgtagtacg gttcgaagag gagcagaccg tcgccgggat tgaggagcgc catgatcgct
     1561 gccgtgaacg cgccggaggc tccgacggtg acgacgatgt cggtctcggg gctggcgtgg
     1621 atgccatttt cgcgcccgag cttgtcggcg atgacacgtc gtagcgtgga ggtcccttcg
     1681 gggaaggagt aggtgctgcg attctcgcgg atggcggtga tcgcgccgtc acgcacgagg
     1741 gggagcgtcg gcagatcgca aatg
//
LOCUS       DASVII010000597         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_495145,
            whole genome shotgun sequence.
ACCESSION   DASVII010000597 DASVII010000000
VERSION     DASVII010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495145"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..460)
                     /gene="rplR"
                     /locus_tag="VF309_07755"
     CDS             complement(101..460)
                     /gene="rplR"
                     /locus_tag="VF309_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7466519.1"
                     /translation="MGIKIPKSRSEKRTRRHLRVRKAVQGTAERPRLVVFRSLKHISA
                     QLVDDDAARTLATVTSTKVGGEGKKSQKSLEVGKQIAVVAKEKGITKVVFDRGGYQYH
                     GRVKAVADGAREGGLEF"
     gene            complement(464..1006)
                     /gene="rplF"
                     /locus_tag="VF309_07760"
     CDS             complement(464..1006)
                     /gene="rplF"
                     /locus_tag="VF309_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7466520.1"
                     /translation="MSRIGKAPITIPKGVTITLADGNVVTVKGPKGELTRTVPAAMQL
                     KQEDGHVVVTRPSEESNHKSLHGLTRTLIANMVEGVTKGFTKNLELVGVGYKAEVRPY
                     GLQLALGYSHAIEYKAPKGIKLSAPVPTQILVEGSNKEVVGQVAAEIRSLRPPEPYKG
                     KGVKYAGEQVRRKAGKAGGK"
     gene            complement(1018..1419)
                     /gene="rpsH"
                     /locus_tag="VF309_07765"
     CDS             complement(1018..1419)
                     /gene="rpsH"
                     /locus_tag="VF309_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7466521.1"
                     /translation="MSMTDPIADMLTRIRNACGSKHRRVDMPVSKMKTEIARILKEND
                     YIQDYATVEAEDGKTVLRVRLKYAAGGQSVIRALNRVSTPGLRKYVGVGEIPRVRNGL
                     GIAILSTSKGLMSDREARQARTGGELLALIW"
BASE COUNT          242 a          546 c          404 g          313 t
ORIGIN      
        1 ccgcgccgcc gccgctacgc gcactgccac cgccaccgct gcgtgctccg ccgccagacc
       61 cgcccggcgc gccgctgcct gaattcgtat tggattccat ttagaactcc agcccgcctt
      121 cgcgagcccc gtcggccacc gctttcacgc ggccgtgata ctggtacccg ccacggtcga
      181 agacgacctt cgtgatcccc ttctccttgg cgaccacggc gatctgcttc ccgacctcga
      241 gcgacttctg tgacttcttc ccttctccgc caaccttggt ggacgtgacg gtcgcgagcg
      301 tgcgcgccgc gtcgtcgtcc acaagttggg cgctgatgtg cttgagcgag cgaaagacga
      361 ccagacgtgg acgctccgct gttccctgta cggccttgcg cacccgcaag tgtcgccgcg
      421 tgcgcttctc cgaccgactc ttcggaatct tgattcccat ggcttacttt cctcccgcct
      481 tgccggcctt gcggcgaacc tgctcgcccg cgtacttgac gcccttcccc ttgtacggct
      541 cgggcggacg caggctgcga atttccgccg ccacctggcc caccacctcc ttgttcgacc
      601 cctcgacgag aatctgcgtc ggcaccggag cgctcaactt gatccccttc ggcgccttgt
      661 actcgatggc gtgcgagtag cccagcgcga gctgcaagcc atacggccgc acttccgcct
      721 tgtagccaac gcccacaagc tcgaggttct tcgtgaagcc cttcgtcacc ccttccacca
      781 tgttggcgat cagggtgcgc gtcaggccgt gcagcgactt gtggttcgac tcctccgacg
      841 gacgcgtgac caccacgtgc ccatcctcct gcttcagctg catcgccgcc gggacggtcc
      901 gggtgagctc acccttcgga cccttcaccg tgacgacgtt gccatctgcc aaggtgatcg
      961 tgacgccctt agggatagtg atgggtgctt tgccaattcg cgacatgagt gggctcctta
     1021 ccagatgagg gcgagaagct cgccgccggt gcgcgcctga cgcgcttccc gatcggacat
     1081 cagccccttc gacgtggaaa ggatcgcgat gcccagcccg ttgcgcacgc gcgggatctc
     1141 gccgaccccg acgtacttcc gaagcccggg cgtcgacaca cggttcagcg cgcggatgac
     1201 cgactggccc cccgccgcat acttgagccg cacgcggagc acagtcttgc catcctcagc
     1261 ctcgaccgtc gcgtaatcct ggatgtagtc gttctccttc aggatgcgcg cgatctcggt
     1321 cttcatcttc gacaccggca tatcgacgcg gcggtgcttc gatccgcaag cattgcggat
     1381 ccgcgtcaac atatcggcaa tcggatcagt catcgacatc gaatcatcac tccccgttat
     1441 tcctcaatcg tcattcgatt aatcgagtaa tcgattaatc gattaatcga ataccgcttt
     1501 accaa
//
LOCUS       DASVII010000598         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_711176,
            whole genome shotgun sequence.
ACCESSION   DASVII010000598 DASVII010000000
VERSION     DASVII010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711176"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF309_07770"
     CDS             <1..237
                     /locus_tag="VF309_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466522.1"
                     /translation="HLIYEALKKTGGVATDGEKLVNAMKGLSWVSPRGKMTIDPATRD
                     VIQTVYIRKCEMVNGKPWNVEFDKIENVKDQGAA"
     gene            263..1414
                     /locus_tag="VF309_07775"
     CDS             263..1414
                     /locus_tag="VF309_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466523.1"
                     /translation="MLGSAAAATAASLPFSARAADPVRVGLILPLTGPFASTGRQIEA
                     GARLYMAKNGDTVAGQKIELLVRDDTGLAPETTKRLAQELIARDKVSFLAGFGLTPLA
                     LATAPLATEAKVPMIVMGAATSIVPQRSPFIVRAGFTVPQITAPIAQWGARNGMKRVV
                     TSVTDFAPGVECEKTFIKFFTEGGGTIVESMRTPLQNPDYGPFLQRAKDAKPDSMFIF
                     VPSGQGAAIMKQFVDRGLKEAGIKLICTGDVLDDDLLEAIGPSALGVVSSHHYSAAHD
                     SPENKAYVEGYLRANNGMRPNFMSVHGYDGIHVIYESLKKTGGNAADGEKLVNAMKGL
                     AWVSPRGPMSIDPATRDVIQNIYIRRCEMVNGKLWNIEFDKTENVKDPG"
     gene            1414..>1704
                     /locus_tag="VF309_07780"
     CDS             1414..>1704
                     /locus_tag="VF309_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466524.1"
                     /translation="MTTRTKPPFRADHVGSFLRPKKLLDARERHAKGEISKPELRKVE
                     DEAIRDIVKFQEDLGLQGITDGEFRRTFFHIDFLEQLDGVETHTGLTSQFHTK"
BASE COUNT          342 a          574 c          541 g          247 t
ORIGIN      
        1 cacctcatct acgaggcgct gaagaagacc ggcggcgtgg caacggacgg cgagaagctc
       61 gtgaacgcga tgaagggcct ttcgtgggtg agcccgcgtg gcaagatgac catcgacccc
      121 gccacgcgcg acgtgatcca gaccgtctac atccgcaaat gcgagatggt gaacggcaag
      181 ccgtggaacg tggagttcga caagatcgag aacgtcaaag accagggggc cgcctgacat
      241 ggacaaatcc agaaggcaat tcgtgcttgg ttcggcggcc gccgcgacgg cggcatcact
      301 gcccttttcg gcgcgcgccg ccgatccggt ccgcgtcggc ctcatcctgc cgctcaccgg
      361 cccgttcgct tcgaccggcc gccagatcga ggccggagcg cgcctgtaca tggcgaagaa
      421 cggcgacacc gtcgccggcc agaagatcga gctgctggtg cgcgacgaca ccggcctcgc
      481 gcccgagacc accaagcgcc tcgcccagga attgatcgcg cgcgacaagg tgagcttcct
      541 cgcgggcttc ggcctcacgc cgctcgcgct cgccaccgcg ccgctcgcga ccgaagcgaa
      601 ggtcccgatg atcgtcatgg gcgcggcgac ttccatcgtg ccgcagcgct cgcccttcat
      661 cgtccgggcg ggcttcacgg tgccgcagat caccgcgccc atcgcgcaat ggggcgcgcg
      721 caacggcatg aagcgcgtcg tcaccagcgt gaccgacttc gccccgggcg tcgagtgcga
      781 gaagaccttc atcaagttct tcaccgaggg cggcggcacg atcgtcgaat cgatgcgcac
      841 gccgctgcag aaccccgact acggcccgtt cctgcagcgt gcgaaggacg cgaagcccga
      901 ctcgatgttc attttcgtgc cttcgggcca gggcgcggcg atcatgaagc agttcgtcga
      961 ccgcggcctg aaggaagcgg gcatcaagct catctgcacc ggcgacgtgc tcgacgacga
     1021 cctgctggag gcgatcggcc cgtccgcgct cggcgtggtg agctcgcacc actactccgc
     1081 cgcgcacgac tcgcccgaga acaaggccta cgtcgaaggc tacctgcgcg ccaacaacgg
     1141 gatgcggcca aacttcatgt cggtgcacgg ctacgacggc attcacgtga tctacgagtc
     1201 gctgaagaag accggcggca acgcggccga cggcgagaag ctggtgaacg cgatgaaggg
     1261 gctcgcgtgg gtgagcccgc gcggcccgat gtcgatcgat cccgccaccc gggacgtcat
     1321 ccagaacatc tacatccggc gctgcgagat ggtgaacggc aagctctgga acatcgaatt
     1381 cgacaagacc gagaacgtaa aggaccccgg ataatgacca cacgcaccaa gccgccgttt
     1441 cgcgccgacc acgtcggcag tttcctgcgc ccgaaaaagc tcctcgacgc acgcgagcgg
     1501 cacgcgaagg gagaaatctc gaagcccgag ctgcgcaagg tcgaggacga agcgatccgc
     1561 gacatcgtga aattccagga ggatttgggg ctccagggca ttaccgatgg cgaattccgc
     1621 cgcacgttct tccacatcga cttcctcgag cagctggacg gcgtggagac ccataccggg
     1681 ctcacctcgc agttccacac gaag
//
LOCUS       DASVII010000599         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_387683,
            whole genome shotgun sequence.
ACCESSION   DASVII010000599 DASVII010000000
VERSION     DASVII010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387683"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VF309_07785"
     CDS             complement(<1..709)
                     /locus_tag="VF309_07785"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7466525.1"
                     /translation="MNAVERLEGNVVAITGASKGIGAELARQLAAKGAKLVLAARNEK
                     ELEQVAADCRKAGASVVVVKADVALERDCQAIVAGAVLAFGRLDTLVNNAGISMWARF
                     EDIEDMSILERIMQVNYMGSVYCTRHALPHLRESRGRIVGVASLAGMIGVPTRTGYSA
                     SKHAMRGFFDSLRIELDGSGVTVTMIYPGFVSTGIRESATGPDGKPILVSPVKEREVM
                     SVEDCSRRIVRAIERRER"
     gene            complement(753..1970)
                     /locus_tag="VF309_07790"
     CDS             complement(753..1970)
                     /locus_tag="VF309_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7466526.1"
                     /translation="MTLRSLLVDFNSYFASVEQQVEPRLRGKPLGVVPMLADTTVCIA
                     ASVEAKTFGVKTGTKVAEARRLCPGIEFVIARHEVYIEYHHRAVAVVDTVVPVRAVLS
                     IDEMDCELTGRWREPARALETAKKVKTAIASQVGEYLRTSIGIGPNTFIAKTASDMVK
                     PDGLVLIEQSELPERLFDLEVRALSGVGKQMEKRLHAHGIRTVRDLAAKSRDEMHAIW
                     GGIGGEIMWSRIRGEEQHERENDTSSISHSSVLGPDNRNPEHAFAVLNRLTQKAAMRL
                     RKAGFYASRLSIDLKYLDGGHWGAEMRLVDTQDTKTFLHALEKLWSKRPRDARTILRV
                     GMAYSDFVGEGDHTGSLFAAEDKSKSLYSTLDKLNSRFGKQAVYFASAHKARDRGGLH
                     IAFNHIPDPKTDK"
     gene            complement(1967..2389)
                     /locus_tag="VF309_07795"
     CDS             complement(1967..2389)
                     /locus_tag="VF309_07795"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7466527.1"
                     /translation="MSYIDESLIEGETLLHRAHVSWWSQFGFVLLGIVTLVVGIGLVF
                     LAIAWIRVRSTEIAITDRRVIFKTGFVTRHTVEINLEKVESLRVEQGLWGRFLNFGSI
                     YISGTGASVAPFHDIADPLVFRRKFMEATNRPITMPRP"
     gene            complement(2392..2886)
                     /locus_tag="VF309_07800"
     CDS             complement(2392..2886)
                     /locus_tag="VF309_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7466528.1"
                     /translation="MFALKSDRPGTKAGLHSELLSQASALLAGERDATANAANLAALV
                     FHTLPDLNWAGFYWMKQGELVLGPFQGKPACVRIAIGAGVCGTAARDKRTVVVEDVHR
                     FPGHIACDSASMSEVVVPLVRDGKVLGVFDLDSPSTRRFDEEDARALEALVAIYLEAS
                     DLGG"
     gene            complement(2921..3157)
                     /locus_tag="VF309_07805"
     CDS             complement(2921..3157)
                     /locus_tag="VF309_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466529.1"
                     /translation="MNTRNTLLAAILAAALPVVAQTTPSPGERNPARVQAVDKATASA
                     QAQSAKQGARTAKSKRKATKKPKKGDQPSAAKAG"
     gene            3297..>4962
                     /locus_tag="VF309_07810"
     CDS             3297..>4962
                     /locus_tag="VF309_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7466530.1"
                     /translation="MPFTTQAALSEAAPPPVAPANPRVTTKFGDRRVDPYAWLRDKRD
                     PAVKRYLEAENRYTDAVMKPLEGLRETLYKEILGRIQETDESAPYRRHGYWYYQREVE
                     GLQYPIYCRRKGAMDAPEEVILDVNALATGHAYTSIGILDVSPDAGKLAYTVDFTGYR
                     QYTLHVKDLSSGELLRDCADRVTSLAWAADNATLFYVQEHKTTKRSHRLYRHRVGRPK
                     NELAHKAELLYEETDELFDIGVCDTRSEAYVVLSALSKDTSEMRVLPTTDPGGRFEVI
                     LPRRAGHEYYLDHRGGEFFIRTNDKGRNFRLVRAPVADPSPRNWRQVVAHRPLVMIEE
                     VHLFRDFWVMVERDKGLIDLRVTDFATGESHYLAFDEKAYCAHPGANYEFDAKRFRFV
                     YESFVTPRSWYDYDVRSRKRTLLKRQPVLGPYDPSEYASEALTAVARDGTRVPISLVY
                     RKSLRAKGAQPLLLYGYGSYGIPMDPWFSSARISLLDRGMIFAIAHVRGGGDRGRLWY
                     DAGKLLVKKNSFTDFIACAESLVRRRYTSPGQLVIQGGSAGGLLMGA"
BASE COUNT          786 a         1656 c         1654 g          866 t
ORIGIN      
        1 cccgctcccg gcgttcgatg gctcttacga tccggcggga acaatcttcg acgctcatca
       61 cttcgcgttc cttcaccggg ctcacgagga tgggcttgcc gtcggggccg gtggcgcttt
      121 cgcgaatgcc ggtcgagacg aagccggggt agatcatcgt gaccgtgacg cccgagccgt
      181 cgagctcgat cctcagcgaa tcgaagaagc cgcgcatggc gtgcttggag gcgctgtagc
      241 cggtgcgcgt gggcacgccg atcatgcccg cgaggctcgc gacgccgacg atgcgcccgc
      301 gcgattcgcg aaggtgcggc agcgcgtggc gcgtgcagta gacggagccc atgtagttca
      361 cctgcatgat cctttcgagg atcgacatgt cctcgatgtc ctcgaagcgc gcccacatgc
      421 tgatgcccgc gttgttcacg agcgtgtcga gccttccgaa cgcgagcacc gcgcccgcga
      481 cgatcgcctg gcagtcgcgc tcgagcgcga cgtcggcctt cacgacgacg acggacgcgc
      541 ccgccttgcg gcagtcggcg gccacctgct cgagctcctt ctcgttcctc gccgcgagca
      601 cgagcttcgc ccccttcgcc gcgagctgcc tcgcaagctc cgcgccgatt cccttggaag
      661 cccctgtaat ggcgacgacg tttccctcga ggcgttcgac ggcgttcatc ggcgcttatc
      721 tgcggattcg aggcttcttg tatgcgtcac tctcatttgt cggtcttcgg gtcgggaatg
      781 tggttgaagg cgatgtgcaa cccgccgcgg tcgcgcgcct tgtgggcgct cgcgaagtag
      841 acggcctgct tgccgaagcg cgagttgagc ttgtcgagcg tcgagtagag gctcttcgac
      901 ttgtcctcgg cggcgaagag ggagcccgtg tggtcgccct cgccgacgaa gtccgagtag
      961 gccatgccga ccctgaggat ggtgcgcgca tcccgcgggc gcttcgacca gagcttctcg
     1021 agcgcgtgca ggaaggtctt ggtgtcctgg gtgtccacga ggcgcatctc ggcgccccaa
     1081 tgcccgccgt cgaggtactt caggtcgatc gacagccgcg aagcgtagaa gcccgccttg
     1141 cgaagccgca tcgccgcctt ctgagtgagg cggttcagca ccgcgaaggc atgctccgga
     1201 ttgcggttgt cggggccgag cacgctcgag tgcgagatcg acgaggtgtc gttctcccgc
     1261 tcgtgctgct cctcgccgcg aatgcggctc cacatgatct cgccgccgat gccgccccag
     1321 atcgcgtgca tctcgtcgcg cgattttgcc gcgaggtcgc gcaccgtgcg gatgccgtgg
     1381 gcatgcaggc gcttctccat ctgcttgccc actcccgata gcgcgcgcac ctcgagatcg
     1441 aagaggcgct ccggcaattc cgattgctcg atcaggacga ggccgtcggg cttcaccatg
     1501 tcggaggcgg tcttcgcgat gaaggtattg gggccgatgc cgatggaggt gcgcaggtat
     1561 tcgccgacct gcgaagcgat ggccgtcttc accttcttcg ccgtctcgag agcgcgcgcc
     1621 ggctcccgcc agcgtcccgt gagctcgcaa tccatctcgt cgatcgagag caccgcgcgc
     1681 accggcacca cggtgtcgac cacggccacg gcgcgatggt ggtattcgat atagacctcg
     1741 tggcgcgcga tcacgaactc gatccccggg cacagccgcc gcgcctcggc gaccttggtg
     1801 ccggtcttca cgccgaaggt cttcgcctcg acgctcgccg cgatgcatac cgtggtgtcg
     1861 gcgagcatcg gcacgacgcc aaggggcttg ccgcgaagcc ggggctctac ctgctgctcg
     1921 acagacgcga aataggagtt gaagtcgacg aggagcgagc gaagcgtcac ggccggggca
     1981 tcgtgatcgg gcggttggtc gcctccatga acttgcggcg gaagacgagg gggtcggcga
     2041 tgtcgtggaa gggcgccacc gacgcgcccg tgcccgagat gtagatcgac ccgaagttga
     2101 ggaagcgtcc ccagaggccc tgctcgaccc gcagcgactc gaccttctcg aggttgatct
     2161 cgacggtatg gcgcgtcacg aatccggtct tgaagatcac gcgccggtcg gtgatcgcga
     2221 tctcggtgga tctcacgcgg atccacgcga tcgcgaggaa gacgaggccg atgccgacga
     2281 cgagcgtcac gatgcccagg agcacgaagc cgaactgcga ccaccacgag acgtgcgcgc
     2341 gatgcaggag cgtttcgccc tcgatcagcg actcatcgat ataggacatc gtcagcctcc
     2401 caggtcgctc gcctcgaggt agatcgcgac cagcgcctcg agggcgcgcg cgtcctcctc
     2461 gtcgaagcgc cgtgtgctgg ggctgtcgag atcgaagacg ccgagcacct tgccgtcgcg
     2521 caccagcgga accacgacct cgctcatcga ggccgaatcg cacgcgatgt gtccgggaaa
     2581 ccggtgcacg tcctcgacga ccacggtgcg cttgtcgcgc gccgcggtgc cgcacacgcc
     2641 cgcgccgatc gcgatgcgca cgcacgcggg cttgccctgg aaggggccga gcaccagctc
     2701 gccctgcttc atccagtaga agcccgccca attgagatcc ggaagcgtgt ggaagacgag
     2761 cgccgcgagg ttcgccgcgt tcgccgtggc gtcgcgctcg cccgcgagaa gcgcgcttgc
     2821 ctgggaaagc agctcggaat ggagaccggc cttcgtgccg ggacggtcgg acttgagggc
     2881 gaacatgaag gcactccgcg aggagtgcct actttaacat tcagccggct ttggccgccg
     2941 agggctggtc gcccttcttg ggcttcttcg tcgcctttct cttcgacttc gcggtcctgg
     3001 cgccctgctt ggcggattgg gcctgggcgc tcgccgtggc cttgtcgacg gcctgcacgc
     3061 gcgccgggtt cctttctccc ggggacgggg tggtttgcgc cacgacgggc agggcggccg
     3121 cgaggatggc cgcgaggagg gtgttgcggg tgttcatttc gattgctcct acgagttgtg
     3181 ggtgtgcctg gggcacgcct cttcaacgga actccggatg acgcgttgac gtccgtataa
     3241 ttcgctccct acgtccgtgg tgctcccgcc tcgcgcccat gcttctttcc gtgcccttgc
     3301 cgttcaccac ccaggctgcc ctctcggaag cggccccgcc gcccgtggcg ccggcgaatc
     3361 cccgggtgac gacgaagttc ggcgaccggc gggtggaccc gtacgcctgg cttcgcgaca
     3421 agcgcgatcc cgcggtcaag cgatatctcg aggccgagaa ccgctacacc gacgcggtga
     3481 tgaagccgct cgagggcttg cgcgagacgc tctacaagga gatcctcggc cgcatccagg
     3541 aaaccgacga gagcgcgccg taccggcgcc acggctactg gtactaccag cgcgaggtcg
     3601 aagggctgca gtacccgatc tactgccgcc gcaagggcgc catggatgcg cccgaggagg
     3661 tgatcctcga cgtgaacgcg ctagccacgg gccacgcgta cacgtcgatc gggatcctcg
     3721 acgtgagtcc cgatgcgggc aagctcgcgt acaccgtgga cttcacgggc tatcgccagt
     3781 acacgctgca cgtgaaggat ctctcgagcg gggagctgct gcgcgactgc gccgatcgcg
     3841 tgacttccct cgcctgggcc gcggacaacg ccacactctt ctacgtccag gagcacaaga
     3901 ccaccaagcg cagccatcgc ctgtatcgcc accgggtcgg aaggccgaag aatgagctcg
     3961 cacacaaggc cgagctgctg tacgaggaga ccgacgagct cttcgacatc ggggtctgcg
     4021 acacgcgaag cgaggcgtat gtggttttgt ccgcgttgag caaggacacc tccgagatgc
     4081 gcgtgctgcc gacgaccgat cccggcgggc gattcgaggt gatcctgccg cgcagggccg
     4141 ggcacgagta ctacctcgac catcggggcg gcgagttctt catccgcacc aacgacaagg
     4201 ggcgcaactt ccgcctggtg cgcgcgcccg tcgccgatcc gtcgccgcgc aactggaggc
     4261 aggtcgtcgc ccatcgcccg ctggtgatga tcgaggaagt ccatctcttc cgcgatttct
     4321 gggtcatggt cgagcgcgac aagggcctca tcgacctgcg cgtcaccgat ttcgcgaccg
     4381 gcgagtccca ctatctcgcg ttcgacgaga aggcctactg cgcgcatccg ggagcgaact
     4441 acgaattcga cgcgaagcgc tttcgcttcg tctacgaaag cttcgtcacg ccgcgctcct
     4501 ggtacgacta cgacgtgcgc tcgcgcaagc gcacgctgct caagcgccag ccggtgctcg
     4561 gtccctacga tccctcggag tacgcgtccg aggcgctcac ggcggtggcg cgggatggca
     4621 cgcgcgtgcc gatctcgctc gtctacagga agtcgctgcg cgcgaagggc gcgcagccgc
     4681 tcctcctcta cgggtatggt tcctacggaa tcccgatgga tccctggttc tcgagcgcgc
     4741 gcatctcgct cctcgaccgc ggaatgatct tcgcgatcgc gcacgtgcgc ggcggcggcg
     4801 atcgcggcag gctctggtac gacgcgggta agctcctcgt gaagaagaac tcgttcacgg
     4861 acttcatcgc gtgcgcggag tcgctggtgc gccggcgcta cacctcgccc gggcagctcg
     4921 tgatccaggg cgggagcgcc ggggggctgc tcatgggggc gg
//
LOCUS       DASVII010000600         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_171428,
            whole genome shotgun sequence.
ACCESSION   DASVII010000600 DASVII010000000
VERSION     DASVII010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171428"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..921
                     /locus_tag="VF309_07815"
     CDS             100..921
                     /locus_tag="VF309_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7466531.1"
                     /translation="MKTLAGKTLFITGGSRGIGREIAMRAARDGANVVIAAKTSDPHP
                     KLAGTIHSVAEEIVKAGGKSLAIQLDVRDDEAIRDAMKRTAEAFGGLDILVNNASAIS
                     LTPTMETPAKRFDLMLGVNARGTFLCSQAAIPLLKKSKNAHILTLSPPLSMKAKWFAG
                     HVAYTMSKYGMSMCTLGMAEELKGFGIAVNSLWPRTTIATAAVEVHFPAAILAASRKP
                     EIMADAAHEILTSDSRTTTGNFYIDEDVLKRAGTTDFAHYAVTPGVPLFQDLFVE"
     gene            complement(926..1153)
                     /locus_tag="VF309_07820"
     CDS             complement(926..1153)
                     /locus_tag="VF309_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX7466532.1"
                     /translation="MQSFDRELDVRGLNCPLPILRAKKALGELTAGQVLKVVATDPGS
                     VKDFQAFCKQTGNELLSHSEGAEFTFFMKKR"
     gene            complement(1213..>1901)
                     /locus_tag="VF309_07825"
     CDS             complement(1213..>1901)
                     /locus_tag="VF309_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX7466533.1"
                     /translation="ASCRAAIRRGRRSAERWRAEFTAMGRALGGGSGWVVLTYVPRDG
                     RLVNQFATDHGQSVAGGIPILALDMYEHAYHMDFGANATAYVDTFMRNLDWKAIEARY
                     GDAKNAPGPRELVQEEWADVPAVGPESIKAMLDSGTPVQVIDARPRHYFTKSQEIMAG
                     AKWRDPDQVDAWVGELSQDKPVVVFCAYGFHVGCRTAQTLRSRGFDATYMAGGHSAWK
                     AIGGKLEMPT"
BASE COUNT          324 a          676 c          590 g          311 t
ORIGIN      
        1 ctttacgtta aacctgcgga ctttccgcac gcacctgcgg accgtctatg tcagccgggg
       61 tatcatcccc gccttggacg acacccgctc aggcaaccca tgaagacgct cgccggaaag
      121 acgcttttca tcaccggggg atcgcgcggc atcgggcgcg agatcgcgat gcgcgccgcg
      181 cgcgacggcg cgaacgtggt catcgccgcg aagacctcgg atccgcatcc gaagctcgcg
      241 gggacgattc attcggtcgc cgaggagatc gtgaaggcgg gcggcaagtc gctcgcgatc
      301 cagctcgacg tgcgcgacga cgaggcgatc cgcgatgcga tgaagcgcac ggcggaagct
      361 ttcggcgggc tcgacattct cgtgaacaac gcgagcgcca tcagcctcac gccgacgatg
      421 gagacgcccg ccaagcgctt cgacctgatg ctgggcgtga acgcgcgcgg gacttttctc
      481 tgctcgcagg cggcgatccc gctgctgaag aaatccaaga atgcgcacat cctcacgctc
      541 tcgccgccgc tgtcgatgaa ggccaaatgg ttcgcggggc acgtcgccta cacgatgtcg
      601 aagtacggga tgagcatgtg cacgctgggg atggccgagg agctgaaggg gttcggcatc
      661 gcggtgaatt cgctgtggcc gcgcaccacc atcgccaccg ccgcggtcga ggtccacttc
      721 cccgccgcca tcctcgccgc cagccgcaag cccgagatca tggccgacgc cgcccacgag
      781 atcctcacca gcgactcccg caccaccacg ggcaacttct acatcgacga ggacgtcctc
      841 aagcgcgccg gcacgaccga cttcgcgcac tacgccgtca cccccggcgt tccgctcttc
      901 caggacttgt ttgtggagta ggaaactacc gcttcttcat gaagaaggtg aactccgcgc
      961 cctcggagtg agacagcagc tcgttgccgg tctgcttgca gaaggcctgg aagtccttca
     1021 cggagccggg gtcggtggcg acgaccttca gcacctggcc ggccgtgagc tcgcccaggg
     1081 ccttcttggc cctgaggatg gggagcgggc aattgagtcc gcgaacgtcg agctcgcgat
     1141 cgaacgactg catcttggtt tctccagcgg ggcccgaatg ttatcacgca agccccgccg
     1201 gagaagaccc gctcacgtgg gcatctctag ctttcccccg atcgccttcc acgccgaatg
     1261 gccgcccgcc atataggtcg cgtcgaagcc ccgcgagcgt agcgtctgcg cggtgcggca
     1321 gcccacgtgg aagccgtagg cgcagaagac caccacgggc ttgtcctgcg agagctcccc
     1381 cacccaggcg tcgacctggt ccgggtcgcg ccacttcgcc cccgccatga tttcctggct
     1441 cttcgtgaag tagtggcgcg gccgtgcgtc gatcacctgc accggcgtcc cggaatccag
     1501 catcgccttg atgctctcgg gccccaccgc cggcacgtcg gcccactcct cctgcacgag
     1561 ctcgcgcggg cccggcgcgt tcttcgcatc gccgtagcgc gcctcgatcg ccttccagtc
     1621 gaggttgcgc atgaacgtgt ccacgtaggc cgtcgcgttc gcgccgaagt ccatgtgata
     1681 ggcgtgctcg tacatgtcga gcgcgaggat cggaatgccg cccgcgaccg actgcccatg
     1741 gtccgtggcg aactggttca cgagccgtcc atcgcgcggc acataggtga ggaccaccca
     1801 tcccgagccg cccccgagcg cgcgtcccat cgcggtgaac tccgcgcgcc agcgctccgc
     1861 cgagcgcctt ccacgccgaa tggccgcccg acatgaagct c
//
LOCUS       DASVII010000601         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_244130,
            whole genome shotgun sequence.
ACCESSION   DASVII010000601 DASVII010000000
VERSION     DASVII010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244130"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF309_07830"
     CDS             <1..369
                     /locus_tag="VF309_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466534.1"
                     /translation="TLRYDYEVKGSNAGVYGLQMPLTDFYAFNGWTLHFYNTPRQGLR
                     DQWLTGRVVAAKATLYAEAHRFGSDFGDIGFGRELDLSIAYPLMENGVLRLQHARYDP
                     GAGTPDPRIRKTWLTFALTY"
     gene            369..1151
                     /locus_tag="VF309_07835"
     CDS             369..1151
                     /locus_tag="VF309_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX7466535.1"
                     /translation="MALSKSELEELRAAKLILEHPGLAAKLSALAGAPIDRGMALMPA
                     RWQKGVHDATSKALMKALDLAVRSLGDKARLSSSDKLHKMAVATSGAIGGAFGIAALG
                     VELPISTGLMLRSIADIAASHGEDPRRMETQLACLTVFALGSPRDSSDDAAESGYFAA
                     RTALAGAVTEATRHLAQKGLAKGTAPPLVRLITLIASRFGVVVSQKAAAQMVPVIGAA
                     GGALINTIFIGHYQDMARGHFIVRRLEKAHGAEPVRLAYEKL"
     gene            complement(1148..1444)
                     /locus_tag="VF309_07840"
     CDS             complement(1148..1444)
                     /locus_tag="VF309_07840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7466536.1"
                     /translation="MSAAAGEIRHEGGERDGSFFIERDGRRIAQLTYRMMGADAMLDH
                     TWVDPPLRGGGEAKRLVDAAADWARSEHVKLVPACSYVRALFARSSAYADVQRR"
     gene            1494..1787
                     /locus_tag="VF309_07845"
     CDS             1494..1787
                     /locus_tag="VF309_07845"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX7466537.1"
                     /translation="MRAYVLAEMEVTDPEGYKGYTAVVGESIRKYGGRFLVRGGATLG
                     LEGEWPARRRVILEFPSMEVAKAWWDSPEYAKPKALRRAHSTGRLMLLEGFDG"
     gene            complement(1795..2169)
                     /locus_tag="VF309_07850"
     CDS             complement(1795..2169)
                     /locus_tag="VF309_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466538.1"
                     /translation="MARRYKPLPPPEVYRRHLATSGSVGLLLIVVSLAVGMAGYAYFE
                     KIGFYDAFLNASMILSGMGPILNPASTGGKVFAGFYALYSGFAVLAIAAIMFAPVVRR
                     FLHRFHLEEIDEDVPKKDPPAK"
     gene            complement(2169..>2877)
                     /gene="hemF"
                     /locus_tag="VF309_07855"
     CDS             complement(2169..>2877)
                     /gene="hemF"
                     /locus_tag="VF309_07855"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX7466539.1"
                     /translation="SDVTGSKLPPSASAHRPEIAGGSWEAMGVSLVLHPRNPHVPTVH
                     MNVRFFVAGHVWWFGGGMDLTPYYPVEEDCAHFHRANRDALARFPGLHARFKKACDDY
                     FFLKHRNEPRGIGGTFFDDFSEGGFERSFEVMRAVGDAFLPAYVPIVGKRRNAPYGER
                     ERDFQAYRRGRYVEFNLVWDRGTLFGLQSGGRAESILMSMPPAVVWRYDWKPAPGSPE
                     AKLASVLMPPRDWVNLA"
BASE COUNT          501 a          974 c          971 g          431 t
ORIGIN      
        1 acgctgcgct acgactacga ggtgaaaggc agcaacgccg gcgtgtatgg gctgcagatg
       61 ccgcttacgg atttctacgc cttcaatgga tggacgctgc acttctacaa taccccgcgc
      121 cagggattgc gcgaccaatg gctcacgggc cgtgtggtcg cggcgaaggc gaccctgtac
      181 gcggaggcac atcgcttcgg ctcggatttc ggcgacatcg gcttcggacg cgagctcgac
      241 ctgagcatcg cctatccact aatggaaaac ggcgtgcttc gtctgcagca tgcccgctac
      301 gacccgggag cgggcacgcc cgacccgcgc atccgcaaga cctggcttac cttcgcgctc
      361 acgtattgat ggctctctcg aaatcagaac tggaagagct tcgcgccgcg aagctgatcc
      421 tcgagcatcc gggcctcgcc gcgaagcttt cggcgctcgc gggcgcgccg atcgacagag
      481 gcatggccct catgcccgcg cgctggcaga agggcgtgca cgacgcgacc tcgaaggccc
      541 tcatgaaggc gctcgacctc gcggtgcgca gcctcggcga caaggcgcgg ctgtcgtcat
      601 cggacaagct ccacaagatg gcggtcgcca cctcgggcgc gatcggcggc gcgttcggca
      661 tcgcggcgct cggcgtggag ctgccgatat cgacggggct catgctgcgc tccatcgcgg
      721 acatcgccgc gagccacggc gaggatcccc gccggatgga gacccagctc gcgtgcctca
      781 cggtcttcgc cctgggaagc ccgagggatt cgagcgacga cgcggccgaa tcgggctatt
      841 tcgcggcgcg caccgcgctc gcgggcgccg tcaccgaagc gacgcggcac ctcgcgcaga
      901 agggcctcgc gaagggcacc gccccgccac tcgtgcgcct catcaccctc atcgcctcgc
      961 gcttcggcgt cgtggtctcg cagaaggcgg cggcgcagat ggtgccggtg atcggcgccg
     1021 cgggcggggc cctcatcaac acgatcttca tcggccacta ccaggacatg gcgcgcggcc
     1081 acttcatcgt gcgccgcctg gagaaggccc acggcgcgga gccggtgcgg ctcgcttacg
     1141 agaagctcta gcgcctctgc acgtcggcgt aagcggacga gcgcgcgaag agcgcgcgca
     1201 cataggagca agccggaacg agcttcacgt gctcgctgcg cgcccaatcg gcagcggcgt
     1261 ccaccaggcg cttcgcttca cctccgccgc gcagcggcgg atcgacccaa gtgtggtcca
     1321 gcatcgcatc ggcacccatc atgcgatagg tgagctgggc gattcgccgg ccgtcgcgct
     1381 cgatgaagaa cgagccgtcg cgttcgccgc cttcgtggcg aatttccccc gccgccgcgc
     1441 tcatggataa tcccctgcaa ccattctcat tggaccgcga aaggagcgaa aagatgcgtg
     1501 cctacgttct cgccgaaatg gaggtcaccg accccgaagg ctacaagggc tacaccgccg
     1561 tggtcggcga atccatcagg aaatacggtg ggcgcttcct ggtgcgcggt ggcgcaaccc
     1621 tcgggctcga gggcgagtgg cccgcgcgcc gccgcgtcat tctcgagttc ccgtcgatgg
     1681 aagtggcgaa ggcctggtgg gattcgcccg agtacgcgaa gccgaaggcc ctgcgccgcg
     1741 cccacagcac cggccggctc atgctgctcg agggcttcga cggatgagcg cgcgctactt
     1801 cgccggcgga tccttcttcg gcacgtcttc atcgatctcc tctaaatgga atcggtgcag
     1861 gaagcggcgc acgacggggg caaacataat tgcggcgatc gcgagcaccg cgaagcccga
     1921 gtagagcgcg tagaaccccg cgaagacctt gccgcccgtc gacgccggat tgaggatcgg
     1981 ccccatgccc gagaggatca tcgacgcgtt gaggaacgcg tcgtagaaac cgatcttctc
     2041 gaagtacgcg tagcccgcca tgccgaccgc gagcgagacg acgatgagga ggagccccac
     2101 ggatccgctc gtggcgagat ggcgccggta gacttccggg ggcggcagcg gcttgtagcg
     2161 tcgggccatc aggcaaggtt aacccaatcg cgcggcggca tcagcaccga ggcgagcttc
     2221 gcttccggcg agcccggcgc gggcttccag tcgtagcgcc acaccacggc gggcggcatc
     2281 gacatgagga tcgattcggc gcgtcccccg gactgcaggc cgaagagcgt gccgcggtcc
     2341 cacacgaggt tgaactccac gtagcggccg cggcgatacg cctggaaatc gcgctcgcgc
     2401 tcgccgtagg gcgcgtttcg gcgcttcccg acgatgggga cgtacgcggg caggaacgcg
     2461 tcgcccacgg cgcgcatcac ttcgaagctg cgctcgaatc ctccctcgct gaaatcgtcg
     2521 aagaacgtgc cgccgatgcc gcgcggctcg ttgcggtgct tgaggaagaa gtagtcgtcg
     2581 cacgccttct tgaagcgcgc gtgcagcccg ggaaagcgcg cgagggcgtc gcggttggcg
     2641 cgatggaaat gcgcgcagtc ctcctcgacc gggtagtacg gcgtgaggtc catgccgccc
     2701 ccgaaccacc agacgtggcc cgcgacgaag aagcgcacgt tcatgtggac cgtcggcacg
     2761 tggggattgc gcggatgcag caccagcgag acgcccatcg cctcccacga gccgccggcg
     2821 atctcgggac gatgcgccga ggccgagggc ggaagcttgc tgccggtcac gtcggag
//
LOCUS       DASVII010000602         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_136027,
            whole genome shotgun sequence.
ACCESSION   DASVII010000602 DASVII010000000
VERSION     DASVII010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136027"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VF309_07860"
     CDS             complement(<1..496)
                     /locus_tag="VF309_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466540.1"
                     /translation="MAANPISDFDLGPLTWVKTEIDHSLNQARETLDKLAANPADRAP
                     VKYILTHLHQATGALAMVGLGAATRFNEELEKLVAFLETEDAGHLPGAVAAAKKAISA
                     LSAYLDTLLAGQPDHPMRLLAPYLELNRARGHSDANEGDLFFPNLGAALPPMAGTDSL
                     DDAVL"
     gene            complement(533..>1840)
                     /locus_tag="VF309_07865"
     CDS             complement(533..>1840)
                     /locus_tag="VF309_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7466541.1"
                     /translation="RDTEARAKLLELKKTFTTFEQNAGPILRELQKLVSARQAGTHLV
                     TASEQLQDTVKALGDELQDQRTATTLIAIIVVGALLVIVLALTGLVFLADTRRRAAQA
                     ETENRRNQEAILRLLNEMGDLADGDLTIRAQVTEDITGAIADSMNYTIDELRTLVAGV
                     NNAAIQVTQKTAQAQSISAELLGAAERQSKEIEETTAQILQVSHSISEVSTTADEGAR
                     VAQRSLAAADKGRAAVQNSISGMNDIREQIQETSKRIKRLGESSQEIGEIVELISDIT
                     EQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSGEATKQIGAIVKTIQADTQD
                     AVAAMEKSTTGVVEGAKLSDAAGQALSEIDFVTKNLAQLIEQISKATHTQASATNKVA
                     QNMQDILEITRQTTRGTQQAAGSIRDLAAVAQELKSSVSGFKL"
BASE COUNT          236 a          602 c          641 g          361 t
ORIGIN      
        1 cgaggaccgc gtcgtcgagg gagtcggtcc cggccatggg aggcagcgcg gcaccgaggt
       61 tcggaaagaa aagatctcct tcgttggcat cgctgtgtcc gcgcgcgcga ttgagctcga
      121 ggtacggcgc gagaaggcgc atggggtgat cgggctggcc cgcaagcagc gtgtcgagat
      181 aggcggacag cgcggagatc gccttcttcg ccgcggccac ggccccggga aggtgccccg
      241 catcctcggt ctcgaggaag gccacgagct tctccaactc ctcgttgaag cgcgtcgccg
      301 cgcccagtcc caccatcgcg agcgcgcccg tggcctggtg caggtgcgtg aggatgtact
      361 tgacgggcgc gcggtcggcg ggattggccg cgagcttgtc gagggtctcg cgcgcctggt
      421 tgagcgagtg gtcgatctcg gtcttgaccc acgtgagggg gccgagatcg aaatcggaga
      481 tcgggttggc tgccatctgg agttcgttcg agctttctct cgccttcgcg ccttagagtt
      541 tgaaacccga caccgagctc ttcagctcct gcgccaccgc ggcgaggtcg cggatcgagc
      601 cggccgcctg ctgcgtgccg cgcgtcgtct ggcgcgtgat ctcgaggatg tcctgcatgt
      661 tctgggcgac cttgttggtg gcgctcgcct gggtgtgcgt cgccttcgag atctgctcga
      721 tcaactgggc caggttcttg gtcacgaagt cgatctcgga cagggcctgt cccgcggcgt
      781 cggagagctt cgcgccctcg accacgcccg tcgtcgattt ctccatggcc gcgacggcgt
      841 cctgggtgtc cgcctgaatc gtcttcacga tggcgccgat ctgcttcgtc gcctctcctg
      901 agcgttcggc gaggcgctgg acctcctccg cgacgaccga gaagccgcgg cccgcctcgc
      961 cggccgatgc cgcctggatc gcggcgttca gcgcgagcac gttcgtctgc tccgtgatgt
     1021 ccgagatgag ctccacgatc tcgccgatct cctgggagct ctcgccgagt cgcttgatgc
     1081 gcttcgaggt ttcctggatc tgctcgcgga tgtcgttcat gcccgagatc gagttctgca
     1141 ccgcggcgcg tcccttgtcg gcggccgcga gcgagcgctg cgcgacgcgc gcgccttcat
     1201 cggccgtcgt ggacacctcc gagatcgagt gcgacacctg cagaatctgc gcggtcgtct
     1261 cctcgatttc cttcgactgc cgctccgcgg cgccgaggag ctcggccgag atcgactgcg
     1321 cctgcgccgt cttctgcgtg acctggatgg cggcgttgtt cacgccggcg acgagggtcc
     1381 gcaactcgtc gatcgtgtag ttcatggaat cggcgatggc gcccgtgatg tcctcggtca
     1441 cctgcgcgcg gatggtgagg tcgccgtcgg cgaggtcgcc catctcgttc agcagccgca
     1501 ggatcgcctc ctggttgcgg cggttctcgg tctccgcctg cgccgcgcgc cggcgcgtgt
     1561 cggcgaggaa cacgaggccg gtcagcgcga gcacgatgac gaggagggcc cccaccacga
     1621 tgatcgctat gagggtggtg gcggtcctct ggtcctgcag ctcgtcgccg agggccttca
     1681 ccgtgtcctg gagctgctcg ctcgcggtga cgaggtgcgt gcccgcctgg cgcgccgaga
     1741 cgagcttctg gagctcgcga agaatcgggc ccgcgttctg ctcgaaggtc gtgaaggtct
     1801 tcttcaattc gaggagcttc gcgcgcgctt cggtatcccg
//
LOCUS       DASVII010000603         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_64198, whole
            genome shotgun sequence.
ACCESSION   DASVII010000603 DASVII010000000
VERSION     DASVII010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64198"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VF309_07870"
     CDS             <1..1210
                     /locus_tag="VF309_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7466542.1"
                     /translation="EGIELAAESRLAGTPGSRKVIKDRKGRIVEDVESLRAPRDGAEV
                     TLAIDQRLQFLAHRELKAAVELNRAKGGSMVILDAKTGEVMALVNQPDYNPNNRANVT
                     GRQTRNRSVTDLFEPGSTMKPFTVAAALDAGIVTPGTLVDTGPGFMTLGGWTINDSHP
                     NGTITVSQVIQRSSNIGSAKIMMQIPAETMGTLYRELGFGLAPQSGFPGEAKGQLKPW
                     TQWRPIEQATMSYGHGLSVSLLQISRAYTIFTNAGELLPLSLLKQDARPIGKRIVSAQ
                     TADQVTRMMEMAAMPGGTAPRAQVSGYRVAGKTGTAHKAELGGYAEHKYVSSFVGFGP
                     VTNPRFIIAVMLDEPMGSKYYGGDVSAPVFSTVMGAALRMMSIPPDAPGTLNVMAPPA
                     ERPGAVKVAG"
     gene            1207..>2153
                     /locus_tag="VF309_07875"
     CDS             1207..>2153
                     /locus_tag="VF309_07875"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7466543.1"
                     /translation="MIAAPEFVRTEGTRLLDRLAQLGVPLADLTADSRAVKLGSIFVA
                     YPGTVRDGRAFIADAIARGATAVLWEHEGFEWNERWGVPNLGVRGLRAQISTIAGHVF
                     GNPSDSLWMAGVTGTNGKTSVSQWIAAALDGLGRRSAVIGTLGNGLVGERSEAKNTTP
                     DPIVLQRLLADYLRRGARAVAMEVSSHGLDQDRVAGIKYDAAVFTNLTRDHLDYHGTM
                     EEYFRAKQVLFEGCGTEPPRAAVINIDDEYGRQLLALSKKKSSLVLSYGLTTGDFHAE
                     SVDITSRGTRFQMITPAGKLAIWSPMIGKVNVYNVLAACA"
BASE COUNT          367 a          740 c          726 g          320 t
ORIGIN      
        1 ggagggcatc gagctcgccg ccgagtcgcg cctggccggc acgcccggct cgcgcaaggt
       61 gatcaaggac aggaagggcc gcatcgtcga ggacgtggag agcctgcgcg ccccgcgcga
      121 cggcgccgag gtgacgctcg ccatcgacca gcgcctgcag ttcctcgcgc accgcgagct
      181 caaggccgcg gtggagctga accgcgcaaa aggcggctcg atggtgatac tcgacgcgaa
      241 gaccggcgag gtgatggcgc tggtgaacca gcccgactac aaccccaaca accgcgccaa
      301 cgtcaccggc aggcagacgc gcaaccgctc ggtcaccgat ctcttcgagc cgggctcgac
      361 gatgaagccc ttcaccgtcg cggcggcgct cgacgcggga atcgtcacgc ccggcacgct
      421 cgtggacacc gggcccgggt tcatgacgct tggcggctgg accatcaacg actcgcatcc
      481 caatggcacc atcaccgttt cgcaggtgat ccagaggtcg tcgaacatcg gcagcgccaa
      541 gatcatgatg cagatcccgg ccgagaccat gggcacgctc taccgcgagc tgggcttcgg
      601 cctggcgccc cagagcggat tcccgggcga ggcgaagggg cagctcaagc cctggacgca
      661 gtggcggccg atcgagcagg cgaccatgtc gtatggccac ggcctgtcgg tgtcgctgct
      721 gcagatctcg cgcgcgtaca cgatcttcac caacgcgggc gagctgctgc cgctctcgct
      781 cctcaagcag gacgcgcgcc cgatcggcaa gcgcatcgtc tccgcccaga ccgcggacca
      841 ggtgacgcgc atgatggaaa tggcggcgat gcccggcggc acggcgccgc gcgcgcaggt
      901 ctccggctat cgcgtcgcgg gcaagaccgg caccgcgcac aaggcggagc tgggcggcta
      961 cgccgagcac aagtacgtgt cgtctttcgt gggcttcggt ccggtgacga acccgcgctt
     1021 catcatcgcg gtgatgctgg acgagccgat gggctccaag tactacggcg gcgacgtgag
     1081 cgcgccggtg ttctccacgg tgatgggcgc tgcgcttcgc atgatgtcga ttcccccgga
     1141 cgcgccgggc acgctcaacg tgatggcccc gccggccgag cgcccgggcg ccgtgaaggt
     1201 ggcgggatga tcgccgcgcc ggaattcgtg cgcaccgaag gcacgcgcct cctcgatcgc
     1261 ttggcgcagc tcggcgtgcc gctcgccgac ctcaccgccg actcgcgcgc ggtgaagctg
     1321 ggctcgatct tcgtcgcgta cccggggacg gtgcgcgacg ggcgcgcgtt catcgccgat
     1381 gcgatcgcgc gcggggcgac cgcggtgctc tgggaacacg aaggcttcga atggaacgaa
     1441 cgctggggcg tgcccaacct cggcgtgcgc ggcttgcgcg cgcagatctc caccatcgcc
     1501 ggccacgtgt tcggcaatcc ctccgactcg ctgtggatgg cgggcgtcac cggcaccaac
     1561 ggcaagacgt cggtgtcgca gtggatcgcc gccgcgctcg acggcctcgg gcggcgctcc
     1621 gcggtgatcg gcacgctcgg caacggcctg gtcggcgagc gcagcgaggc gaagaacacc
     1681 acgccggatc cgatcgtgct gcagcgactg ctcgccgact acctgcgccg cggcgcgcgc
     1741 gcggtcgcga tggaagtgtc gagccacggc ctggaccagg accgcgtggc gggcatcaag
     1801 tacgacgccg cggtcttcac caacctcacg cgcgaccacc tcgactatca cggcacgatg
     1861 gaagagtact tccgcgccaa gcaggtactg ttcgaaggtt gcggaacgga gccaccccgc
     1921 gccgcagtca tcaatatcga cgacgaatac ggccgccagt tgctcgctct gagcaaaaag
     1981 aagagttcgt tagttctttc ctacggcctg accaccggtg attttcacgc ggaatccgtc
     2041 gacatcacct cgcgtggcac ccgcttccag atgattactc ctgctggcaa attagcgatc
     2101 tggtcgccca tgatcggcaa ggtgaatgtg tacaacgtcc tggccgcttg tgc
//
LOCUS       DASVII010000604         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_387811,
            whole genome shotgun sequence.
ACCESSION   DASVII010000604 DASVII010000000
VERSION     DASVII010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387811"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VF309_07880"
     CDS             <1..603
                     /locus_tag="VF309_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7466544.1"
                     /translation="STKEHRLDKNPLVLLELVERLGNLMRTELRKAGGEESLQPVQLQ
                     ALVYLSRANRYSNTPQALADYLGLTKGTVSQTLLLLDRRGLIERFEDDVDRRVVRLRL
                     STSGERLLYETQPALPWQNATRNISPNRVRNAVSALREALMTLQQDNEATTFGDCQSC
                     TWCQKLSQRIYRCGQMGDRLSGPETRKICRIYAPKVEEVT"
     gene            complement(613..>1929)
                     /gene="argH"
                     /locus_tag="VF309_07885"
     CDS             complement(613..>1929)
                     /gene="argH"
                     /locus_tag="VF309_07885"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7466545.1"
                     /translation="RFNASISFDRRLAEFDIQGSLAHARMLARCGLLSPGDLAAIERG
                     LAQILADVQADRFAWSVEREDVHLNIEHRLTELVGDAGKRLHTARSRNDQVATDLRLW
                     LRTQIDELAALLRRVRKGLLDLAEKHTGTLMPGFTHMQVAQPVTFGHHLMAYFEMFSR
                     DAERFADCRRRVNRLPLGASALAGTSFPIDRESVAKELGFDGVCENSLDAVSDRDFAV
                     EFLAAAALVMAHVSRLSEELVIWMSPGFGFVRIADRFCTGSSIMPQKKNPDVPELMRG
                     KTGRVYGSLVAMLTVIKGQPLAYNKDNQEDKEPLFDTADTLRDVLALLAELLKGIEPD
                     AQRMRQALRKGYATATDLADYLVRKGVAFRDAHEIVARAVRAAEESGRDLSELGVEAL
                     RAFSPLISDDVLAALTPEGSVAARNHIGGTAPEAVRRAIARARKRL"
BASE COUNT          326 a          648 c          665 g          290 t
ORIGIN      
        1 tcgacgaagg agcatcgcct ggacaagaac ccgctcgtcc tgctcgagct ggtcgaaagg
       61 ctcggcaacc tgatgcgtac ggagttgcgc aaagccggtg gcgaagagtc gctgcagccg
      121 gtccagcttc aggcgcttgt ctatcttagc agggcgaacc gctactcgaa tacgccgcag
      181 gccctcgccg actacctcgg gctcaccaag gggacggtct cgcagacgct gctgctgctc
      241 gatcgccgcg gattgatcga gcgcttcgag gacgacgtcg accgccgggt ggtgcggctg
      301 cgcctctcga cctcgggcga gcggctcctc tacgagacgc agcccgcgtt gccgtggcag
      361 aacgccacgc gcaacatcag cccgaaccgc gtgcgcaacg ccgtgtccgc gctgcgcgag
      421 gcgctgatga cgctgcagca ggacaacgag gcgaccacgt tcggggattg ccaaagctgc
      481 acgtggtgcc agaagctatc gcagcgcatc taccgctgcg gccagatggg cgatcggctg
      541 tcggggccgg agacgcgcaa gatctgccgc atctacgctc ccaaggtcga ggaagtgacc
      601 tgaaggcgcg gcctagaggc gcttgcgagc ccgcgcgatc gcccggcgaa ccgcttccgg
      661 cgccgtcccg ccgatgtgat tgcgcgccgc gaccgatcct tcgggcgtga gggccgccag
      721 gacgtcgtcg gagatgagcg gcgagaaggc gcgcagcgct tcgactccga gctccgagag
      781 gtcgcgtccg gattcctccg cggcgcgcac cgcgcgcgcc acgatctcgt gggcgtcgcg
      841 aaacgcgacg cccttgcgca ccaggtagtc cgcgagatcc gtggcggtgg cgtatccctt
      901 ccggagcgcc tgcctcatgc gctgcgcatc cggctcgatc cccttcagca actcggcgag
      961 aagcgcgagg acatcgcgga gcgtgtcggc ggtatcgaaa agcggctcct tgtcctcctg
     1021 gttgtccttg ttgtacgcga ggggctgccc cttgatcacc gtgagcatgg ccacgagcga
     1081 gccgtagacg cgccccgtct tgccgcgcat gagctcagga acgtccggat tcttcttctg
     1141 cggcatgatc gaggagccgg tgcagaaccg atccgcgatg cggacgaatc cgaacccggg
     1201 gctcatccag atcacgagct cctccgacag gcgcgagacg tgcgccatca cgagcgccgc
     1261 ggcggcgagg aattcgaccg cgaagtcccg gtcggagacg gcgtcgagcg agttctcgca
     1321 gacaccgtcg aagcccagct ccttcgcgac cgactcgcgg tcgatgggaa agctcgttcc
     1381 cgcaagggcc gaagcgccca ggggaagccg gttcacgcgc ctgcggcaat cggcaaagcg
     1441 ctccgcgtcc cggctgaaca tctcgaaata agccatcagg tggtggccga aggtgaccgg
     1501 ctgggcgacc tgcatgtgcg tgaagcccgg catgagcgtc ccggtgtgct tctcggcaag
     1561 gtcgaggagg cccttgcgca cgcgcctgag aagcgccgca agctcgtcga tctgcgtccg
     1621 cagccacaac cggaggtcgg tcgccacctg gtcgttgcgc gagcgcgccg tgtgcagccg
     1681 cttgcccgca tccccgacca gctccgtcag gcgatgctcg atgttgaggt gcacgtcctc
     1741 gcgctcgacg gaccaggcga accggtcggc ctggacgtcc gcgaggatct gcgccaggcc
     1801 gcgctcgatg gcggcgaggt ccccggggga aagcagcccg cagcgcgcga gcatgcgcgc
     1861 gtgcgcgagc gagccctgga tgtcgaattc cgcaaggcgg cggtcgaagg agatcgacgc
     1921 gttgaagcg
//
LOCUS       DASVII010000605         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_279888,
            whole genome shotgun sequence.
ACCESSION   DASVII010000605 DASVII010000000
VERSION     DASVII010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279888"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF309_07890"
     CDS             <1..646
                     /locus_tag="VF309_07890"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7466546.1"
                     /translation="APGGKDVNATVVYGVNHKTLKSSHTVISNASCTTNCLAPLVKPL
                     HDAIGLVNGLMTTIHAYTNDQVLVDVFHEDLRRARSATHSMIPAKTGAAAAVGLVLPE
                     LNGKLDGFAIRVPTINVSLVDLSFVAARDTTADEVNSIMKKASEAGDLKGILGYSTGP
                     LVSVDFNHDPRSSIFDATLTKVSGRLVKVCSWYDNEWGFSNRMLDVTLALMQAK"
     gene            661..>1638
                     /locus_tag="VF309_07895"
     CDS             661..>1638
                     /locus_tag="VF309_07895"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7466547.1"
                     /translation="MAELDLAGRRVFIRSDLNVPMDDAGKITDDTRIRASLAAYRLAL
                     EKGAAVMATSHLGRPKEGEFSEADSLVNVAKRIGELLGREVALKRDWVEGVDVKPGEM
                     VLLENCRFNKGEKKDDETLARKYAALCDVYVNDAFGTAHRAEASTHGVAQYAKVACAG
                     PLLAAEIDALGRALAKPRRPLVAIVAGSKVSTKLTILKSLSARVDQLIVGGGIANTFL
                     LAAGLPIGKSLCEADLAPEAKEILVLMEARGAAVPIPTDVVCGKSFAADTPAQTKPAR
                     EVDDDDMILDIGPETSARYAKLLQEAGTIVWNGPVGVFEFDAFGNGTRTV"
BASE COUNT          288 a          562 c          554 g          234 t
ORIGIN      
        1 cgcgcccggc ggcaaggacg tcaacgcgac cgtggtctac ggcgtgaacc acaagacgct
       61 gaagagctcg cacaccgtga tctccaacgc ctcgtgcacc accaactgcc tcgcgccgct
      121 ggtgaagccg ctgcacgacg cgatcggcct ggtgaacggg ctcatgacga cgatccacgc
      181 gtacaccaac gaccaggtgc tggtcgacgt cttccacgag gacctgcggc gcgcgcgctc
      241 ggcgacgcac tcgatgattc ccgccaagac cggcgccgcc gcggccgtgg gcctggtgct
      301 gccggagctg aacggcaagc tcgacggctt cgcgatccgc gtgccgacga tcaacgtctc
      361 gctggtcgac ctctccttcg tcgccgcgcg cgacacgacc gccgacgagg tgaactcgat
      421 catgaagaag gcctccgagg cgggcgacct gaagggcatc ctcggataca gcacggggcc
      481 cctcgtctcg gtcgatttca accacgatcc gcgctcgtcg atcttcgacg cgacgctcac
      541 caaggtgtcg ggcaggctgg tgaaggtctg cagctggtac gacaacgagt ggggcttctc
      601 caaccgcatg ctcgacgtca cgctcgcgct gatgcaagcg aagtgaccgg cattctcagg
      661 atggcggagc tcgacctcgc cggccggcgc gtcttcatcc gctccgacct gaacgtgccg
      721 atggacgacg ccgggaagat caccgacgac acgcgcatcc gcgcctcgct cgcggcgtac
      781 cggctcgcgc tcgagaaggg cgcggcggtg atggcgacgt cgcacctcgg ccggccgaag
      841 gaaggcgaat tctcggaagc ggactcgctc gtgaatgtcg cgaagcggat cggcgagcta
      901 ctgggccgcg aggtggcgtt gaagcgcgac tgggtggaag gcgtcgacgt gaagccgggc
      961 gagatggtcc tcctcgagaa ctgccgcttc aacaagggcg agaagaagga cgacgagacg
     1021 ctcgcgcgca agtacgccgc gctctgcgac gtctacgtga acgacgcgtt cggcaccgcc
     1081 catcgcgcgg aagcctcgac ccacggcgtg gcgcagtacg cgaaggtggc gtgcgcgggc
     1141 ccgctcctcg cggcggagat cgacgccctt ggccgcgcgc tggcgaagcc caggcgtccg
     1201 ctcgtagcga tcgtcgcggg atcgaaggtc tccaccaagc tcacgatcct caagtcgctc
     1261 tcggcgagag tcgaccagct catcgtgggc ggcggcatcg ccaacacctt cctgctcgcc
     1321 gcggggctgc ccatcggcaa gtcgctgtgc gaggcggacc tcgcgcccga ggcgaaggag
     1381 atcctggtgc tgatggaggc gcgcggcgcg gcggtgccga ttcccaccga cgtggtctgc
     1441 ggcaagagct tcgccgccga cacgcccgcg caaacgaagc ccgcgcgcga agtcgatgac
     1501 gacgacatga tcctcgacat cggcccggag acttccgcgc gctacgcgaa gctcctgcag
     1561 gaagccggaa ccatcgtctg gaacgggccc gtgggcgtct tcgagttcga cgcgttcggc
     1621 aacggcaccc gcaccgtg
//
LOCUS       DASVII010000606         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_171653,
            whole genome shotgun sequence.
ACCESSION   DASVII010000606 DASVII010000000
VERSION     DASVII010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171653"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VF309_07900"
     CDS             <1..845
                     /locus_tag="VF309_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466548.1"
                     /translation="RALITSRLGFANEQLQELNALKDKNQKLVEVLARKAGVERGRIE
                     QARAMMSGLRTVHNRHSADLERLLDPALARESALRAREAVAGSAFSKDIGEILNAFFV
                     DSRERITRAIEVIQEARKVMGGVRQKMTVEYKIAPVEAAAFATERFLVELDRLEEHCR
                     REFKGGSNLLLRSRKSLGTLFFDTVALQVVHVFEIADRETRAWLTGFIRPLESQINAY
                     QDQSNARIEGMGRIQNAEVDLLDKLEELRRLAAEVGAQREQCEAHQQRLMSLLEVERE
                     PSLA"
     gene            complement(849..1112)
                     /locus_tag="VF309_07905"
     CDS             complement(849..1112)
                     /locus_tag="VF309_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX7466549.1"
                     /translation="MALTITDICINCDVCEPECPNAAISMGPEIYVIDPRRCTECVGH
                     FERPQCREVCPVDCIPLDPRHVETREQLLDKYRGLMLEKAAAG"
     gene            complement(1115..1609)
                     /gene="coaD"
                     /locus_tag="VF309_07910"
     CDS             complement(1115..1609)
                     /gene="coaD"
                     /locus_tag="VF309_07910"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7466550.1"
                     /translation="MTRAVYPGTFDPITNGHEDLIQRASRLFGHVVVGVAHSQAKRPF
                     FSLDERVSLASEVLAPYKNVSVLGFSGLLSQLVKEQSAGVILRGLRAVSDFEYEFQLA
                     GMNRVLAPGVETLFLTPSDKYLFLSATIVREIAVLGGDISAFVHPLTAQRMQVKIGRG
                     ELGD"
     gene            complement(1612..>1827)
                     /locus_tag="VF309_07915"
     CDS             complement(1612..>1827)
                     /locus_tag="VF309_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX7466551.1"
                     /translation="RTPEKFDIAFIDPPFASDLAVRAMAQLRDRLQPGARVYVESGAA
                     LAPPEWVRLVREDRAGAVRYALYEIIS"
BASE COUNT          323 a          599 c          629 g          276 t
ORIGIN      
        1 tgcgcgccct catcacgagc cggctcggct tcgcgaacga gcagctccag gagctcaacg
       61 cgctgaagga caagaaccag aagctcgtcg aggtcctcgc gcgcaaggcg ggcgtcgagc
      121 gcggccgtat cgagcaggcg cgggcgatga tgagcgggct gcgcaccgtg cacaaccggc
      181 actcggccga cctggagcgg ctgctcgatc cggcgctcgc ccgcgagtcg gcattgcgcg
      241 cccgcgaagc ggtcgcgggc agcgccttct cgaaggacat cggcgagatt ctcaacgcct
      301 tcttcgtcga ttcgcgcgag aggatcaccc gagcgatcga ggtcatccag gaggcgcgca
      361 aggtcatggg cggggtgcgc cagaagatga cggtggagta caagatcgcg ccggtggaag
      421 cggccgcatt cgcgacggag cgcttcctgg tggagctcga ccggctcgag gagcactgcc
      481 ggcgcgaatt caagggcggc tccaatctcc tgctgcgatc gcgcaagtcg ttgggcacgc
      541 tcttcttcga taccgtcgcg ctgcaagtgg tccacgtctt cgagatcgcc gaccgggaga
      601 cgcgcgcgtg gctcacgggc ttcatccggc ccctcgaatc gcagatcaat gcgtaccagg
      661 atcagtcgaa cgcccgcatc gaaggaatgg gacggatcca gaacgccgag gtcgacctcc
      721 tcgacaagct cgaggagctc aggcgcctcg cggccgaggt cggcgcccag cgcgagcaat
      781 gcgaggcgca ccagcagcgc ctcatgtccc ttctggaagt ggagcgcgag ccctccctcg
      841 cgtagccgtc agcccgccgc ggccttctcg agcatcagtc cccggtattt gtcgaggagc
      901 tgctcgcggg tttcgacgtg ccgcggatcg aggggaatgc aatccacggg gcacacctcg
      961 cggcactgcg gccgctcgaa gtggccgacg cattcggtgc agcggcgcgg gtcgatcacg
     1021 tagatctcgg ggcccatcga gatcgcggcg ttcgggcact cgggctcgca cacgtcgcag
     1081 ttgatgcaga tatcggtgat cgtgagggcc acgctcagtc gcccagctcg cctcggccga
     1141 tcttgacctg catgcgctgc gcggtgagcg ggtgcacgaa ggccgagatg tcgccgccca
     1201 gcaccgcgat ctcgcgaacg atcgtcgccg agaggaacag gtacttgtcg gaaggcgtca
     1261 ggaagagggt ctccacgccg ggcgcgagca cgcggttcat cccggcgagc tggaactcgt
     1321 attcgaaatc ggacaccgcg cgcaacccgc gcaggatcac gccggccgac tgctccttca
     1381 ccagctgcga gagcagcccc gagaatccga ggaccgacac gttcttgtag ggcgcgagga
     1441 cttcgctcgc gagcgatacg cgctcgtcga gcgagaagaa cgggcgcttc gcctggctgt
     1501 gggcgacgcc gaccacgacg tggccgaaga gccgcgacgc ccgttgaatc aggtcctcgt
     1561 ggccgttcgt gatcggatcg aaggtcccgg gatagacggc gcgcgtgatg gtcatgaaat
     1621 gatctcgtaa agagcatagc gtaccgctcc ggcgcgatct tcgcgcacga ggcgcaccca
     1681 ttcgggcggc gcgagcgcgg cgccgctctc gacgtacacg cgcgcgccgg gctggagccg
     1741 gtcccgcaac tgcgccatcg cccgaaccgc gaggtcgctc gcgaaaggcg gatcgatgaa
     1801 cgcgatgtcg aatttctccg gggtcct
//
LOCUS       DASVII010000607         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_603495,
            whole genome shotgun sequence.
ACCESSION   DASVII010000607 DASVII010000000
VERSION     DASVII010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603495"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..843
                     /gene="rpiA"
                     /locus_tag="VF309_07920"
     CDS             175..843
                     /gene="rpiA"
                     /locus_tag="VF309_07920"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX7466552.1"
                     /translation="MDANAQKKAAAEAALQFIDDDMTVGVGTGSTVNFFIDALASKRA
                     RVKTTVASSEASAQRLRALRIPVSELNDTDGCDVYVDGADEVTEHLAMIKGGGAALTR
                     EKIVAAASRRFVCIADASKLVGVLGRFPLPVEVIPMARSFVARQLVKLTGGVPELRAG
                     VTTDNGNLVIDVRGLSILKPMELEADIGLIAGVVENGIFARRPADVLLLGTDQGVRTL
                     NRPK"
     gene            complement(857..1126)
                     /locus_tag="VF309_07925"
     CDS             complement(857..1126)
                     /locus_tag="VF309_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX7466553.1"
                     /translation="MARTVNCIKLKKEAEGLDHPPYPGELGKRLYESVSKEAWKAWLE
                     HQKMLVNENRLNLMDPQARKYLEEQMRKHFFGDGADAAQGYVPKA"
     gene            complement(1144..>1831)
                     /gene="argA"
                     /locus_tag="VF309_07930"
     CDS             complement(1144..>1831)
                     /gene="argA"
                     /locus_tag="VF309_07930"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX7466554.1"
                     /translation="TAGALDAKGKLISELTVREAGELMASNNLPEDVQVYLPCAIKAC
                     SQGVKRSHLISRHVDGALLIELFTHHGIGTMVVPESPEHIRVATLEDIPGIVQILEPL
                     EQQGVLVKREREALERDIGRFFVIESEGNILGCTALYPFPDRQTAELAALAVNPFYRD
                     GGRGERLLAHAEAKARATGLKSIFVLSTRTTHWFLERGFVETDPGWLPERKQALYNYD
                     RRSKVFVKEI"
BASE COUNT          293 a          599 c          613 g          326 t
ORIGIN      
        1 ggatttggtt tcccatgcgc ttcgagaggg cgagcgccgc ctcgagcgga gtttcgatcg
       61 ccacgtcgta gaccttggcg gtgaggatct tctcgagata gccgtctttc ttcatgcggg
      121 ctccgtgggc atcgccgaag catagcaagg cgcgaggcct gctactcttg cggcatggac
      181 gccaatgcgc agaagaaggc ggcagccgag gcggcgctgc aattcatcga cgacgacatg
      241 accgtcggcg tcgggaccgg ctccacggtg aatttcttca tcgacgccct cgcctcgaag
      301 cgcgcccgcg tgaagaccac cgtggcgagc tcggaagcga gcgcgcagcg cctgcgcgcc
      361 ctgcgcatcc cggtctcgga gctgaacgac accgacggct gcgacgtcta cgtcgacggc
      421 gcggacgagg tcaccgagca tctcgcgatg atcaagggcg gcggcgcggc gcttacgcgc
      481 gagaagatcg tcgccgcggc gagccgcagg ttcgtgtgca tcgccgatgc gagcaagctc
      541 gtcggcgtcc tgggccggtt tccgctgcct gtcgaagtga ttccgatggc gaggagtttc
      601 gtcgcccgcc agctggtgaa gctcaccggc ggcgttccgg aattgcgcgc cggcgtgacc
      661 accgacaacg gcaacctcgt catcgacgtt cgcgggctgt ccatcctgaa gccgatggag
      721 ctcgaggccg acatcggcct catcgccggg gtggtcgaga acggcatctt cgcgcgccgc
      781 cccgccgacg tcctcctgct cggcaccgat cagggcgtgc ggacgctcaa ccgcccgaaa
      841 taggacggag ataaggtcaa gccttgggga cgtagccctg cgcggcgtcg gcgccgtcgc
      901 cgaagaagtg cttgcgcatc tgctcctcga ggtacttgcg cgcctgcggg tccatgaggt
      961 tcaggcgatt ctcgttcacc agcatcttct ggtgctcgag ccacgccttc cacgcctcct
     1021 tggagacgct ttcatagagc cgcttgccga gctcgcccgg atagggaggg tggtcgaggc
     1081 cctcggcctc cttcttcagc ttgatgcagt tcaccgtgcg cgccatggcc atgcctccaa
     1141 tttctagatt tccttcacga ataccttgga ccggcggtcg tagttgtaga gcgcctgctt
     1201 gcgctcgggc agccaccccg gatcggtctc cacgaagccg cgctcgagaa accagtgcgt
     1261 ggtgcgcgtg gagagcacga agatcgactt gagccccgtc gcgcgcgcct tcgcttcggc
     1321 gtgcgcgaga agcctctcgc cgcgaccgcc gtcgcggtag aaaggattca ccgcgagggc
     1381 ggcgagctcg gcggtctggc ggtccggaaa ggggtagagc gcagtgcagc cgaggatgtt
     1441 gccctcggat tcgatcacga agaagcggcc gatgtcgcgc tcgagagcct cgcgctcgcg
     1501 cttcacgagc acgccctgct gctcgagggg ctcgaggatc tgcacgatgc ccgggatgtc
     1561 ctcgagcgtc gcgacgcgga tgtgctcggg cgactccggc accaccatcg ttccgatgcc
     1621 gtggtgcgtg aacagctcga tcagcaacgc gccgtcgacg tggcggctga tgagatgcga
     1681 gcgcttcact ccctgggagc acgccttgat cgcgcaggga aggtagacct gcacatcctc
     1741 gggaaggttg ttggacgcca tgagctcgcc cgcctccctc accgtgagct ccgagatgag
     1801 cttgcctttc gcgtcgagcg cgcccgcggt c
//
LOCUS       DASVII010000608         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_603503,
            whole genome shotgun sequence.
ACCESSION   DASVII010000608 DASVII010000000
VERSION     DASVII010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603503"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="VF309_07935"
     CDS             <1..1686
                     /locus_tag="VF309_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7466555.1"
                     /translation="AKLLSARLVALGAIPIAVFCAVYVLARAFLGSTTVTAQAWTLSM
                     ALLAALASLFVLGSLLARVVVGEVEMVREAVRNVTGGVQQTLPELSEPLENLKQEVLG
                     VGETIKAQHGRLQERLDTARQQIFFLTNHDALTGLPNRKALEERLESALSTATSQGST
                     HALLYVDIDFFHRINDSFGHLAGDELLRRITPVMQSSLREGELLARIGGDEFAVLLEN
                     CSAEFAHSAATQLRDAVQGWQFQWNEKAFQVGVSVGVVAITRLAPSLSAILSEADTAC
                     FTAKEQGRNRVFAFHDASSSQYMRQTSRGWLKRINDALTEGAFTLLYQPIVPIDAPHL
                     VGVPRVEALLRMSETGGELILPMSFIPVAERYDLMRTIDRWVVERAFSDYRRLAKMRN
                     ERTPAEFSINLSGHTLSSPDFAQFMQEKFAEFDVPPQAIFFEITETAAIANVERARAL
                     IEALRAMGVRFLLDDFGSGLSSFNYLKHFPVDGIKIDGLFVKGIANNYLDYALVESIH
                     KIATSLGLQTIAEYVETEEIARKLVQIGIVMGQGFHLSPPRAWETLFEKEPAV"
     gene            1725..2087
                     /locus_tag="VF309_07940"
     CDS             1725..2087
                     /locus_tag="VF309_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7466556.1"
                     /translation="MRPTRILALAAGLCIAACALASQAADNNKVTVENRIAQCQGCHG
                     IPNWKTAFPEVYRVPKLGGQKQAYIAAALKQYKTGERDFATMRAIAADLSDADIEQIA
                     KYYGSDAVSTTAASAESK"
BASE COUNT          372 a          751 c          667 g          329 t
ORIGIN      
        1 gcgaaactcc tctccgcgcg gctcgtcgcg ttgggcgcca ttcccatcgc ggtgttctgc
       61 gcggtgtacg tgctggcgcg tgccttcctc ggctcgacga cggtcactgc ccaggcgtgg
      121 acgctgtcga tggcgctcct cgccgcgctg gcgagcctgt tcgtgctggg ctcgctcctc
      181 gcgagagtcg tcgtcgggga agtcgagatg gtgcgcgagg cggtgcgcaa cgtcaccggc
      241 ggagtgcagc agacgctgcc cgagctctcc gagccgctgg agaacctcaa gcaggaggtg
      301 ctcggggtcg gagagacgat caaggcgcag catgggcggc tccaggagcg gctcgacacc
      361 gcgcgccagc agatcttctt cctcaccaac cacgacgcgc tcacggggct gccgaaccgc
      421 aaggcgctcg aggagcgcct cgaaagcgcg ctctccacgg cgacgagcca gggcagcacc
      481 catgcgctcc tctacgtcga catcgatttc ttccaccgca tcaacgattc gttcggccac
      541 ctcgcgggcg acgagctgct caggcgcatc acgccggtca tgcagtcgag ccttcgcgag
      601 ggcgaattgc tcgcgcgcat cggcggcgac gagttcgcgg tgctcctcga gaactgcagc
      661 gcggaattcg cccactcggc ggcgacgcag ctgcgcgacg cggtgcaggg ctggcagttc
      721 cagtggaacg agaaggcctt ccaggtcggc gtgagcgtgg gcgtcgtcgc gatcacgcgc
      781 ctcgccccga gcctctcggc gatcctttcc gaagccgaca ccgcgtgctt caccgcgaag
      841 gagcagggga ggaaccgcgt cttcgccttc cacgacgcga gctcctcgca gtacatgcgc
      901 cagacgagcc gcgggtggct caagcgcatc aacgacgccc tcaccgaggg ggcgttcacg
      961 ctgctgtacc agccgatcgt gccgatcgac gcgccgcacc tcgtcggtgt gccgcgggtg
     1021 gaggccctgc tgcgcatgag cgagaccggc ggcgagctga tcctgccgat gtcgttcatc
     1081 ccggtggccg agcgctacga cctcatgcgc accatcgacc gctgggtcgt cgagcgcgcc
     1141 ttctccgact accgccggct cgcgaagatg cgcaacgaga ggacgcccgc cgaattctcg
     1201 atcaacctct cggggcacac gctctcgagc cccgacttcg cgcagttcat gcaggagaag
     1261 ttcgccgaat tcgacgttcc cccgcaggcc atcttcttcg agatcacgga gacggcggcg
     1321 atcgccaacg tcgagcgcgc ccgcgcgctg atcgaggcgc tgcgcgccat gggcgtgcgc
     1381 ttcctcctcg acgacttcgg cagcgggctc tcgtcgttca actacctcaa gcactttccc
     1441 gtggacggca tcaagatcga cggcctcttc gtgaagggaa tcgccaataa ctacctcgac
     1501 tacgcgctgg tcgagtcgat ccacaagatc gcgacgagcc tcgggctgca gaccatcgcc
     1561 gaatacgtgg aaaccgagga gatcgcccgc aagctcgtgc agatcgggat cgtcatgggc
     1621 cagggattcc acctctcgcc gccgcgcgcc tgggaaaccc tcttcgaaaa ggagcctgct
     1681 gtataattcg cggctgccga atgacaacga agaactccag cgccatgcga cccaccagaa
     1741 tcctcgccct cgccgcgggc ctatgcatcg ccgcgtgcgc cctcgcctcc caggcggcgg
     1801 acaacaacaa ggtcaccgtc gagaaccgga tcgcccagtg ccagggctgc cacggcatcc
     1861 ccaactggaa gaccgccttc cccgaggtct accgcgtgcc gaagctgggc ggccagaagc
     1921 aggcctacat cgccgcggcg ctcaagcagt acaagaccgg cgagcgcgat ttcgcgacca
     1981 tgcgcgccat cgcggcggac ctgtcggacg ccgacatcga gcagatcgcc aagtactacg
     2041 gctccgacgc cgtctccacc acggccgctt cggccgaatc gaaatgaacg cggccatgaa
     2101 accgagactc ctcgccatc
//
LOCUS       DASVII010000609         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_675569,
            whole genome shotgun sequence.
ACCESSION   DASVII010000609 DASVII010000000
VERSION     DASVII010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675569"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF309_07945"
     CDS             complement(<1..342)
                     /locus_tag="VF309_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX7466557.1"
                     /translation="MDLSVWVTYFIATIILSLSPGPGVFSSISSGLHHGFRLGLWNGV
                     GMQVANVFMVVVVAFGLGAILLASETLFTAVKWLGVIYLVYLGIVTWRAAPKTCEEDR
                     DDNAHTAREVFM"
     gene            complement(342..1136)
                     /locus_tag="VF309_07950"
     CDS             complement(342..1136)
                     /locus_tag="VF309_07950"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7466558.1"
                     /translation="MKDDVVAITGASKGIGAELARQLAARGARLVLAARSEKELEAVA
                     EDCRKAGASVLCLRADVASERDCQAIVAGAVLAFGRLDTFVNNAGVSMWARFEDIEDM
                     SILERIMHVNYMGAVYCTKHALPHLRESRGRLVGIASLAGLVGVPTRSGYSASKHAMR
                     GFFDSLRIELDGSGVTVTMIYPGFVSTGIRENASGPDGKPIEVSPVKEGEVMSVEDCA
                     RRIVKAIEGREREVVMTARGKMGLWLKLIAPGLIDRLAKRAIERGR"
     gene            complement(1133..>1638)
                     /locus_tag="VF309_07955"
     CDS             complement(1133..>1638)
                     /locus_tag="VF309_07955"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX7466559.1"
                     /translation="GADTHSISHSSVLGPDNRNPEHAFAVLNRLTQKAAMRLRKAGYY
                     AGRLAIDLKYLDGTHWSTEMRLVDTQDTVTFLHALDKLWARRPKSRRTILKVGMAYSD
                     FVAEADHTGSLFAAEGKSKSLYSTLDKLNARFGKQAVYFASAHKARDRGGMHIAFNHI
                     PDPKTDK"
BASE COUNT          257 a          557 c          543 g          281 t
ORIGIN      
        1 catgaagact tcgcgcgccg tgtgggcgtt gtcgtcgcgg tcctcctcgc acgtcttcgg
       61 cgcggcgcgc cacgtgacga tccccaggta gacgaggtag atcacgccca gccatttgac
      121 cgcggtgaag agcgtctcgg acgcgagcag gatcgcgccc aggccgaacg ccaccacgac
      181 caccatgaag acgttggcga cctgcatgcc cacgccgttc cagagcccca gccgaaagcc
      241 gtggtgcagc ccgctcgaga tgctcgagaa gacgcccggc cccggcgaca gcgacaggat
      301 gatcgtcgcg atgaaatagg tgacccagac cgacagatcc atcaacgtcc tctttcgatg
      361 gcccgcttcg cgagccggtc gatgagtccg ggggcgatga gcttgagcca gaggcccatt
      421 ttaccgcgcg cggtcatgac cacctcgcgc tcgcggccct cgatcgcctt cacaatgcgg
      481 cgcgcgcaat cctccacgct catcacctcc ccttccttca cggggcttac ctcgatcggc
      541 ttgccgtcgg gcccgctcgc gttctcgcgg atgccggtgg agacgaagcc gggatagatc
      601 atggtgaccg tcacgccgct gccgtcgagc tcgatcctca gcgaatcgaa gaagccgcgc
      661 atggcgtgct tgctcgcgct gtagccgctg cgcgtgggca cgccgaccag gcccgcgagg
      721 ctcgcgatgc cgaccaggcg cccgcgcgac tcgcgcaggt gcggcagcgc atgcttggtg
      781 caatacaccg cgcccatgta gttcacgtgc atgatgcgct cgaggatcga catgtcctcg
      841 atgtcctcga agcgcgccca catgctcacg cccgcgttgt tgacgaacgt gtcgaggcgc
      901 ccgaaggcga gcaccgcgcc ggcgacgatc gcctggcaat cgcgctcgct ggcgacgtcg
      961 gcgcgcaagc acagcaccga ggcgccggcc ttgcggcaat cttccgcgac cgcttccagc
     1021 tccttctcgc tgcgcgcggc gaggaccagg cgcgcgcctc tcgcagccag ctggcgagcc
     1081 agctccgcgc cgatgccctt cgatgccccc gtgatcgcta cgacgtcgtc tttcatttgt
     1141 cggtcttggg atcggggatg tggttgaagg cgatgtgcat cccgccgcgg tcgcgcgcct
     1201 tgtgcgcgct cgcgaagtag accgcctgct tgccgaagcg cgcgttcagc ttgtcgaggg
     1261 tcgagtagag gctcttcgac ttgccctcgg cggcgaagag cgagccggtg tggtcggcct
     1321 cggcgacgaa gtccgaatag gccatgccca ccttgagtat ggtgcgccgg ctcttcggac
     1381 ggcgcgccca cagcttgtcg agcgcatgca ggaaggtgac cgtgtcctgc gtatcgacca
     1441 ggcgcatctc ggtcgaccag tgggtgccgt cgaggtactt caggtcgatc gccaggcgtc
     1501 cggcgtagta gccggccttg cgaaggcgca tcgccgcttt ctgggtgaga cggttcagca
     1561 ccgcgaacgc gtgctcggga ttgcggttgt cgggaccgag cacgctcgag tgggagatgg
     1621 agtgcgtgtc ggcgccgc
//
LOCUS       DASVII010000610         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_28887, whole
            genome shotgun sequence.
ACCESSION   DASVII010000610 DASVII010000000
VERSION     DASVII010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28887"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1626
                     /locus_tag="VF309_07960"
     CDS             <1..>1626
                     /locus_tag="VF309_07960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX7466560.1"
                     /translation="ALAGILAQQHASGMESGFGFSARPDAKDSTRWLAQLSQGGLGLP
                     DRDYYFLDDARSKLIRDSYRKHVERMFALAGDGEALAARNAAATIALETELARASMTA
                     VERRDVDRTYNKMTVAELTAFAPGFAWKDYFDALGAGSVTELNVVQPEFAKGFARLAA
                     TRDAADWQAYLRWHVLHATADKLPRPFAKAHFDFYDAVLRGGKEEPPRSRQVIDIIGG
                     RTGTEPMGQALSTVYIENAFSAEAKARVLALVANLKAALAGRLATLEWMSEGTRRRAL
                     EKLAAMRVKVGYPDRWRDYTGADVGAHSFAENWMNAKKYWLRVTLARIGSPVDREEWF
                     TSPHITNAFYNRQGNEIVFPAGILQPPFFDPKADDASNYGAIGAVIGHEITHGFDDGG
                     RRFDAVGNMTDWWTGEDARRYVERAQRVERQYGAFVGVEDIKVDGKLTLGENISDVGG
                     LKIAYLALRKSMAGAPREKVDGLSAEQRFFVSFAQAWRSSYRPELERLQLRTDGHSPP
                     RFRVAGVIANMPEFSSAFGCDASRALLSEGDRA"
BASE COUNT          256 a          572 c          559 g          239 t
ORIGIN      
        1 cgcgctggcg ggaatcctcg cgcagcagca tgcgagcggc atggaatccg gattcggctt
       61 ctcggcgcgt cccgacgcga aggactccac gcgttggctt gcgcagctct cgcagggcgg
      121 gcttggcctg cccgaccgcg attactactt cctcgacgat gcgcgctcga agctgattcg
      181 cgactcgtac cgcaagcacg tggagcgcat gtttgccctc gcgggcgacg gagaggcgct
      241 cgccgcgcgc aatgccgccg cgacgatcgc cctcgagacc gagctggccc gggcgtccat
      301 gacggcggtc gaaaggcgcg acgtcgaccg tacctacaac aagatgaccg tggcggagct
      361 caccgcgttc gcgccgggct tcgcgtggaa ggactatttc gacgcgctgg gcgcgggatc
      421 ggttaccgag ctgaacgtgg tgcagccgga gtttgccaag ggcttcgcac gcctggccgc
      481 gacgcgcgac gccgccgact ggcaggcgta cctgcgctgg cacgtgctgc acgcgaccgc
      541 cgacaagctg ccgcgcccgt tcgccaaggc gcacttcgat ttctacgacg cggtcctgcg
      601 cgggggcaag gaagagccgc cgcgctcgcg ccaggtgatc gacatcatcg gcgggcgcac
      661 cggcaccgag ccgatgggcc aggccctttc aacggtctac atcgagaacg ccttctccgc
      721 cgaggccaag gcgcgcgtgc tcgcgctggt cgccaacctg aaggcggcgc tcgccgggcg
      781 cctcgccacg ctcgaatgga tgagcgaagg cacgcgccgg cgcgccctcg agaagctcgc
      841 cgcgatgcgc gtgaaggtcg gctaccccga tcgctggcgc gactacaccg gcgccgacgt
      901 gggggcccat tccttcgccg agaactggat gaacgccaag aagtattggc tgcgcgtcac
      961 gctcgcgcgc atcggcagcc cggtggaccg cgaggagtgg ttcacctcgc cgcacatcac
     1021 caacgcgttc tacaaccgcc agggtaacga gatcgtgttc ccggcgggca tcctgcagcc
     1081 gccgttcttc gaccccaagg ccgacgacgc ctccaactac ggcgcgatcg gcgcggtcat
     1141 cggccacgag atcacgcacg gcttcgacga cggcggccgg cgcttcgacg ccgtgggcaa
     1201 catgacggac tggtggaccg gggaggatgc gcgccgctac gtggagcgcg cgcagcgcgt
     1261 cgagcggcaa tacggcgcct tcgtgggcgt cgaggacatc aaggtggacg gcaagctcac
     1321 gctcggcgag aacatctccg acgtgggcgg gctcaagatc gcctatctcg cgctgcgcaa
     1381 gtcgatggcc ggggcgccgc gcgaaaaggt cgacgggctt tccgccgagc agcgcttctt
     1441 cgtgtcgttc gcgcaggcgt ggcgcagctc ctaccggccg gagctcgagc gcctccagct
     1501 tcgcaccgac ggccactcgc cgccgcgctt ccgcgtcgcg ggcgtgatcg ccaacatgcc
     1561 cgagttctcc tcggccttcg ggtgcgacgc ctcccgcgcg ctgctttccg agggagatcg
     1621 cgccaa
//
LOCUS       DASVII010000611         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_423765,
            whole genome shotgun sequence.
ACCESSION   DASVII010000611 DASVII010000000
VERSION     DASVII010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423765"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /locus_tag="VF309_07965"
     CDS             complement(<1..1826)
                     /locus_tag="VF309_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX7466561.1"
                     /translation="MRLASDINPAAPLANPPILGEESSTQQQERYADYKIIRRNGAVV
                     GFEPDKIAVAMTKAFLAVSGNTAAASAGMREKVQSLTEAVVQSLMRRHPAGGTFHIED
                     VQDQVELAMMRAGEHQVARSYVLYREERARERAKRVQEAVESRAPRIHVMVGGAKEPL
                     DTNKLEVLITSACEHLGADVDPKKIMDATLRDLYDGVPLEEVHKCAILAARMLLEQDP
                     DYTYVTARLLLHTIRREVLGEEVLHEEMSTRYAEYFPTFVSQGVKAQLLDERLASFDL
                     KRLGGALKPDRDLQFNYLGLQTLYDRYFLHINEKRIELPQAFFMRVAMGLSLNEINRE
                     DRAIEFYNVLSTFDFMSSTPTLFNAGTLRSQLSSCYLTTVADDLDGIYEAIKENALLS
                     KFAGGLGNDWTPVRALGSYIKGTNGKSQGVVPFLKVVSDTAVAVNQGGKRKGAVCCYL
                     ETWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVMEGGDWTLFSPSDVPDL
                     HDKFGKAFEEAYTIYEKKAARGEIKLHKKIPAQSLWRKMLTMLFETGHPWITFKDAAN
                     IRSPQSHVGVVHSSNLCCIAADQRVVTDRGMLTIGELYALGG"
BASE COUNT          297 a          597 c          631 g          371 t
ORIGIN      
        1 ttcccgccca acgcgtacag ctcgccgatc gtgagcattc cgcgatcggt gaccacgcgc
       61 tgatcggcgg cgatgcagca caagttcgaa gagtgcacga cgcccacgtg gctctgcggg
      121 ctgcggatgt tggccgcgtc cttgaacgtg atccacggat gccccgtctc gaagagcatc
      181 gtgagcatct tgcgccacag gctctgggcg ggaatcttct tgtggagctt gatctcgcca
      241 cgcgcggcct tcttctcgta gatcgtgtag gcctcctcga aggccttgcc gaacttgtcg
      301 tgcagatccg gtacgtcgga gggcgagaag agcgtccagt cgccgccctc catcacgcgc
      361 ttcatgaaga ggtcggggat ccagttcgcc gtgttcatgt cgtgggtgcg gcggcgatcg
      421 tcgcccgtgt tcttgcgcag ctcgaggaac tcctcgatgt cgaggtgcca cgtctcgagg
      481 tagcagcaca ccgcgccctt cctcttgccg ccctgattta ccgcgaccgc ggtgtccgag
      541 acgaccttga ggaacggaac cacaccctgg gatttgccat tcgtcccctt gatgtaggag
      601 cccagggcgc gcaccggcgt ccagtcgttt ccgagtccgc cggcgaactt gctgaggagc
      661 gcgttttcct tgatggcttc atagatcccg tccaggtcgt cggcgaccgt cgtgaggtag
      721 cacgacgaca gttgcgagcg cagcgtgccc gcgttgaaga gggtcggcgt cgacgacatg
      781 aagtcgaagg tcgacagcac gttgtagaac tcgatcgcgc gatcctcgcg gttgatctcg
      841 ttcagcgaca ggcccatggc gacgcgcatg aagaacgcct gcggcaactc gatgcgcttc
      901 tcgttgatgt gcaggaagta gcggtcgtag agcgtctgca ggccgaggta gttgaactgc
      961 aggtcgcgat cgggcttcaa cgcgccgccg aggcgcttca ggtcgaagga ggcgaggcgc
     1021 tcgtcgagga gctgcgcctt cacgccctgg ctcacgaagg tcgggaaata ttcggcgtag
     1081 cgcgtcgaca tttcctcgtg cagcacctcc tcgcccagca cttcgcggcg gatcgtgtgc
     1141 agcagcaggc gcgcggtgac gtaggtgtag tcgggatcct gctcgagcag catgcgcgcg
     1201 gcgaggatcg cgcacttgtg cacttcctcg agggggacgc cgtcgtagag atcgcgcagg
     1261 gtcgcgtcca tgatcttctt gggatcgacg tcggcgccga ggtgctcgca cgccgaggtg
     1321 atgagcactt cgagcttgtt cgtgtcgagc ggctccttcg cccctcccac catcacgtgg
     1381 atgcgtggcg cgcgcgattc gaccgcttcc tggacgcgct tggcgcgctc gcgggcgcgc
     1441 tcctcgcgat agagcacgta ggaacgcgcg acctggtgct cgcccgcgcg catcatcgcg
     1501 agctcgacct ggtcctgcac gtcctcgatg tggaaagtgc cgcccgcggg atggcggcgc
     1561 atgagcgact gcaccacggc ctcggtgagc gactggacct tctcgcgcat gccggcgctc
     1621 gccgcggcgg tgttgcccga gacggcgagg aacgccttgg tcatggcgac cgcgatcttg
     1681 tcgggctcga agcccaccac cgcgccattg cggcgaatga tcttgtaatc cgcgtatctc
     1741 tcctgctgct gcgtactgct ctcctccccg agaatgggcg ggttggcgag cggggcggcg
     1801 ggattgatat cggatgccaa gcgcatggga actctcctga aaaactgttt taagtcccgg
     1861 ggttgggcgc cttcccccgg ctctgcctgc cgcggg
//
LOCUS       DASVII010000612         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_495677,
            whole genome shotgun sequence.
ACCESSION   DASVII010000612 DASVII010000000
VERSION     DASVII010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495677"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /gene="iscX"
                     /locus_tag="VF309_07970"
     CDS             complement(<1..135)
                     /gene="iscX"
                     /locus_tag="VF309_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEX7466562.1"
                     /translation="MKWIDTRDIAIALADRFPGFDPATVRFTELRQWVMALDGFDDDP
                     N"
     gene            complement(132..473)
                     /gene="fdx"
                     /locus_tag="VF309_07975"
     CDS             complement(132..473)
                     /gene="fdx"
                     /locus_tag="VF309_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEX7466563.1"
                     /translation="MSVLTVLPHETLCPTGARIETSPGVSICDALLENHIEIEHACEK
                     SCACTTCHVIVREGYESLEPPTEKEDDLLDKAWGLELTSRLSCQARMKDDPVTIEIPK
                     YTINHASELKR"
     gene            complement(470..2341)
                     /gene="hscA"
                     /locus_tag="VF309_07980"
     CDS             complement(470..2341)
                     /gene="hscA"
                     /locus_tag="VF309_07980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEX7466564.1"
                     /translation="MGLLQISEPGDSPEPHQRKLAVGIDLGTTHSLTATVRSGAAEVI
                     ADGEGRPLLPSIVRYFADGQVDVGYDARAAQAEDPANTIVSVKRFMGRGVADVAKYGA
                     LPYHFKDAPGMVSIETAAGPRSPVQVSAEILKVLRERSERALGGELFGAVVTVPAYFD
                     DSQRQATKDAAKLAGLNVLRLLNEPTAAAIAYGLDHASEGTFAIFDLGGGTFDISILK
                     LTRGVFEVLSTNGDSALGGDDFDQAVATHWRVGHRIADAKGGDTRRLLAAAREVKEKL
                     SFEQEAGVQVTLSYGQSLRLVLTRSEFEKITAALVQRPLAPTRKALRDAKLEPADIDG
                     VVLVGGATRMPHLQRAVESHFGKPPLKDLDPDAVVALGAAIQANVLAGNRAAGDDWLL
                     LDVIPLSLGLETMGGLVERVIPRNSTIPVARAQEFTTFKDGQSAMALHVVQGERELVA
                     DNRSLARFELRGIPPMVAGAARIQVTFQVDADGLLSVAAREATTGVESSIVVKPSYGL
                     SDDEVARMLQESFSHAIEDRDARALAEQRVEAESLLAAIRAALDQDADLLGEDERADL
                     DRRMAGLERAWNATDHRAIKAAIEALNRASEAFATRRMDRAVSGALAGRRLDEVASR"
     gene            complement(2341..2874)
                     /gene="hscB"
                     /locus_tag="VF309_07985"
     CDS             complement(2341..2874)
                     /gene="hscB"
                     /locus_tag="VF309_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEX7466565.1"
                     /translation="MTPDFSRDHFELLGLPVRFAVDAARLERGYRDLQSQVHPDRFAA
                     ATEAERRVAMQWATRANEAYRTLRDPIERARYLLHLRGYDTEEESNTSMPADFLMQQM
                     EWREAVDVARDAGDVSELERLRGDIRRSRDEMLVMLERALDADANFVAGCSLVRKLRF
                     LEKLEAEIDSALELTEH"
     gene            complement(2909..>3058)
                     /locus_tag="VF309_07990"
     CDS             complement(2909..>3058)
                     /locus_tag="VF309_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX7466566.1"
                     /translation="GVRVLVDPKSLAYLDGTELDFVREGLNEGFKFNNPNEKDRCGCG
                     ESFNV"
BASE COUNT          452 a         1060 c         1057 g          489 t
ORIGIN      
        1 attcggatcg tcgtcgaatc cgtcgagcgc catcacccac tggcgcagct cggtgaagcg
       61 caccgtggcc ggatcgaagc cgggaaacct gtcggccagg gcgatcgcga tgtcgcgcgt
      121 gtcgatccac ttcatcgctt caattccgac gcatggttga tggtgtattt ggggatctcg
      181 atggtaaccg gatcgtcctt catgcgcgcc tggcacgaca gccgcgaggt gagctcgagg
      241 ccccacgcct tgtcgaggag atcgtcctcc ttctccgtgg gcggctcgag cgactcgtag
      301 ccttcgcgca cgatcacgtg gcacgtcgtg cacgcgcagc tcttctcgca cgcgtgctcg
      361 atctcgatgt ggttctcgag gagcgcgtcg cagatggaga ctccggggct cgtctcgatg
      421 cgcgcgcccg tggggcaaag ggtctcgtgc gggagcaccg tcagcacgct catcgcgagg
      481 cgacttcgtc caggcggcgc ccggcgagcg cgccggaaac ggcgcggtcc atgcgccgcg
      541 tggcgaacgc ctcgctcgcg cggttgagcg cctcgatcgc cgccttgatc gcgcgatggt
      601 cggtcgcgtt ccatgcgcgc tcgagccccg ccatgcgccg gtcgagatcg gcgcgctcgt
      661 cctcgccgag gagatccgcg tcctggtcca gcgcggcgcg gatcgccgcg agcagcgact
      721 ccgcctcgac gcgctgctcg gctagcgcgc gcgcgtcccg gtcctcgatc gcgtgcgaga
      781 aactttcctg cagcatgcgc gcgacctcgt cgtccgaaag cccgtacgag ggcttcacga
      841 cgatcgagga ttcgacgccc gtggtcgcct cgcgcgccgc taccgacagc aggccgtcgg
      901 cgtccacctg aaaggtgacc tggatgcgcg cggcgccggc gaccatcggg ggaatgcccc
      961 gcagctcgaa gcgcgcgagg gagcggttgt cggcgaccag ctcgcgctcg ccctgcacga
     1021 cgtgcagcgc catcgcgctc tgcccatcct tgaaggtcgt gaactcctgc gcgcgcgcga
     1081 ccgggatcgt ggaattgcgc gggatcacgc gctcgacgag cccgcccatc gtctccaggc
     1141 ccagcgacag cggaatcacg tcgagaagaa gccagtcatc gcccgcggcg cgattgcccg
     1201 cgagcacgtt cgcctggatg gccgcgccga gcgcgacgac ggcgtcggga tcgaggtcct
     1261 tcagcggagg ctttccgaaa tggctctcca ccgcccgctg caggtgcggc atgcgcgtgg
     1321 cgcctcccac gagcaccacg ccgtcgatgt cggcgggctc gagcttggcg tcgcgcaggg
     1381 ccttgcgcgt cggggccagc ggccgctgca ccagcgccgc cgtgatcttc tcgaattcgg
     1441 agcgcgtgag gacgaggcga aggctctgcc catacgagag cgtcacctgg acgccggctt
     1501 cctgctcgaa cgagagcttc tccttcacct cccgcgcggc ggcgaggagc cttcgcgtgt
     1561 cgccgccctt cgcgtcggcg atgcggtgcc cgacgcgcca gtgggtcgcc accgcctggt
     1621 cgaaatcgtc gccgcccagc gcggagtcgc cgttggtcga gagcacctcg aagacgccgc
     1681 gcgtgagctt caggatggag atgtcgaagg tgccgccgcc gagatcgaag atcgcgaagg
     1741 tgccttccga ggcgtgatcg aggccgtagg cgatcgccgc ggccgtcggc tcgttcagca
     1801 ggcgcagcac gttcaggccc gcgagcttcg ccgcgtcctt ggtcgcctgg cgctgcgagt
     1861 cgtcgaaata cgcgggcacc gtgaccacgg cgccgaagag ctcgccgccc agcgcgcgct
     1921 cgcttcgctc gcgcagcacc ttgaggattt ccgcggacac ctgcaccggc gagcgcgggc
     1981 ccgcggccgt ctcgatgctc accatgccgg gcgcatcctt gaaatgatag gggagggcgc
     2041 cgtacttggc gacgtcggcg acgccgcggc ccatgaatcg cttcacggag acgatggtgt
     2101 tggcgggatc ctcggcttgc gccgcgcgcg cgtcgtatcc cacgtcgacc tggccatcgg
     2161 caaagtagcg cacgatcgag ggcaggagcg gccgcccctc gccgtcggcg atgacctcgg
     2221 ccgcgccgct gcgcaccgtc gccgtcagag agtgggtggt gcccagatcg attccgaccg
     2281 cgagcttgcg ctggtgcggc tcgggggaat cgcccggctc ggagatctgc aggagcccca
     2341 tcaatgctcc gtgagctcga gggccgaatc gatctccgcc tcgagcttct cgaggaagcg
     2401 cagtttccga acgagggagc acccggcgac gaagttggcg tcggcgtcga gggcgcgctc
     2461 gagcatgacg agcatctcgt cgcgcgagcg ccggatgtcg ccgcgcaggc gctcgagctc
     2521 gctcacgtca ccggcgtcgc gggcgacgtc gaccgcttcg cgccactcca tctgctgcat
     2581 caggaaatcc gcgggcatcg aggtgttgct ttcctcctcg gtgtcgtagc cgcgcaggtg
     2641 aagcaggtag cgcgcgcgct cgatggggtc gcgcagcgtg cgatacgcct cgttcgcgcg
     2701 ggtggcccac tgcatcgcca cgcgccgctc ggcctcggtc gccgcggcga agcggtccgg
     2761 atgcacctgc gattgcaggt cgcgatagcc gcgctcgagg cgcgcggcat ccacggcgaa
     2821 gcgcaccggc aggcccagca actcgaagtg atcgcgagaa aagtcggggg tcatctggac
     2881 acacaaaacc ggtgaccgct ccccggcctc agacgttgaa cgattcgccg cagccgcagc
     2941 ggtccttctc gttggggttg ttgaacttga agccctcgtt caacccttcg cggacgaaat
     3001 cgagctccgt gccgtcgagg tacgcgaggc tcttcggatc gacgaggacg cgcacgcc
//
LOCUS       DASVII010000613         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_352083,
            whole genome shotgun sequence.
ACCESSION   DASVII010000613 DASVII010000000
VERSION     DASVII010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352083"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VF309_07995"
     CDS             complement(<1..676)
                     /locus_tag="VF309_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7466567.1"
                     /translation="MLDIQLLRKDLPAAVAGLKRRGFDFDEAGFRALEDERKKLQSRT
                     EELQAKSNALNKQVGMLKGKGEDASAVLAEVSGLRDGLKANEAALAAVQAKQEAFLRV
                     IPNIPRAEVPDGASEKDNVEVRRWGTPRAFDFAAQDHVDVGTGLGGLDFDTAAKLSGA
                     RFSVLKGGVARLHRALAQLMLGVHTEEHGYTEVQPPLLVRSEIPYGTGNLPKSAEDMF
                     QTSRRLT"
     gene            complement(702..1286)
                     /locus_tag="VF309_08000"
     CDS             complement(702..1286)
                     /locus_tag="VF309_08000"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7466568.1"
                     /translation="MTNRLLLACILSCAIVSPAEAALKAGDSAPDFDAQASLAGKASG
                     YSLKAALKKGPVVVYFYPSAYTSGCNVQAHAFAVNDEKFAAAGASIIGVSLDSIARLN
                     DFSKDPEYCNSKFPVASDADGRIAKSYDLAVKEGRAGIKDTRGVDIDHGFAERTTFVV
                     SPDGKIAATIGGMSPQANVEKALEAVQLLPRGTK"
     gene            complement(1365..>1554)
                     /locus_tag="VF309_08005"
     CDS             complement(1365..>1554)
                     /locus_tag="VF309_08005"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX7466569.1"
                     /translation="KAGVVVGESPAYPFPDAHAHGVQSEDEPTYDVRFRSEDLWPNSA
                     EPALIHVGVFQSYLERAG"
BASE COUNT          249 a          521 c          507 g          277 t
ORIGIN      
        1 gcgtcagccg acggctagtc tggaacatat cctccgccga cttcggcaga ttgccggtgc
       61 cgtatggaat ctcgcttcgg accagtagcg gcggctgcac ttcggtgtag ccgtgctcct
      121 cggtgtgcac gccgagcatg agctgcgcaa gagcgcggtg caggcgcgcg acaccgcctt
      181 tcagcaccga gaagcgcgcg cccgagagct tggccgcggt gtcgaagtcg aggccgccca
      241 gcccggtgcc cacgtcgacg tgatcctgcg ccgcaaaatc gaatgcgcgc ggcgtgcccc
      301 agcggcgcac ctcgacattg tccttctcgg aggcgccatc gggcacttcg gcgcgcggga
      361 tgttggggat cacgcgcagg aaggcctcct gcttcgcctg caccgcggcg agcgccgcct
      421 cgttggcctt caggccgtcg cgcagacccg agacctcggc cagcacggcg gaagcgtcct
      481 cgcccttgcc cttcagcatg ccgacctgct tgttgagcgc gttgctcttg gcctgcagct
      541 cctccgtgcg cgattgcagc ttcttgcgct cgtcctcgag cgcgcggaaa ccggcctcgt
      601 cgaaatcgaa gccgcggcgt ttcaggcccg cgacggcggc gggaaggtcc ttccggagga
      661 gttggatatc gagcatgccg ccattttagc ttcttccccc gctacttcgt tccgcgcggc
      721 agcaactgca cggcctcgag ggctttctcc acgttggcct ggggcgacat cccgccgatg
      781 gtcgcggcga tcttgccgtc gggcgacacg acgaaagtcg tgcgctcggc gaagccgtga
      841 tcgatgtcca cgccgcgcgt gtccttgatg ccggcgcgcc cctccttcac cgcgaggtcg
      901 tacgacttcg cgatgcgccc atcggcatcc gaagccacgg gaaacttgct gttgcagtat
      961 tccgggtcct tcgagaagtc gttgaggcgc gcgatgctgt cgagcgacac cccgatgatg
     1021 ctggcgcccg cggccgcgaa cttctcatcg ttcacggcga acgcatgcgc ctggacgttg
     1081 catccgctgg tgtacgccga aggatagaaa tacacgacca ccggaccttt cttgagggcc
     1141 gccttgagcg aatagccgct cgctttcccg gcgagagagg cctgcgcgtc gaaatcggga
     1201 gcggaatcgc ccgccttgag cgcggcctcg gccggcgaaa cgatggcgca agaaagaatg
     1261 caggcaagca gaaggcgatt cgtcatggga tctccgtgat tatgtgcggc cccggcgcgg
     1321 ggacgcattg caaacatgga tccggcgcgg cttattccgg ctcttcagcc ggccctctcg
     1381 agatagctct ggaagacgcc cacatggatg agcgcgggct ccgcggaatt cggccacagg
     1441 tcctcggagc ggaatcgcac gtcataggtg ggctcgtcct cggactgcac gccgtgcgca
     1501 tgcgcgtccg gaaacgggta tgcgggcgat tcacccacca cgacaccggc cttg
//
LOCUS       DASVII010000614         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_244641,
            whole genome shotgun sequence.
ACCESSION   DASVII010000614 DASVII010000000
VERSION     DASVII010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244641"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF309_08010"
     CDS             complement(<1..303)
                     /locus_tag="VF309_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7466570.1"
                     /translation="MSALEVRGLACSRGGIRLFRDVSFAIAPGEWVAVRGHNGSGKTT
                     LLRCAAGLTRCDAGEVLWDGRDARDAPGPFRAQLLYAGHAAGIKDDLTALENLREAL"
     gene            complement(300..1538)
                     /gene="ccmI"
                     /locus_tag="VF309_08015"
     CDS             complement(300..1538)
                     /gene="ccmI"
                     /locus_tag="VF309_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX7466571.1"
                     /translation="MSVFWIMAALMTAVALSFVLVPLLRARSRAGPTAVAANLDVLRG
                     QRREIDADVANGLLPASSRDEALAELVDRAQDDLAAIPAAVPGDARKSWTAAVVVAVA
                     LPAAAFGIYLALGTPSALDTGAIAHDGGNLDDKQILAMVESLAAKVRKRPDDAQGWAL
                     LARSMAALGRFKEAVDAYEHLLTLVPRDPQTLADYADVLGMAQGRTLAGRPYEIAKEA
                     LSIDPLHKKALALAGTAAMDENDFAAALGHWQALAAQLPPGSDDERQVRAIIGELRTK
                     ATAAGKVLPAERIAKAPVPASVPGSVSGSVSVAPPLTAQVSNAATLFIFARAEGGARM
                     PLAVLRRSASELPMSFALDDSMAMTPATKISGAPAVRIEARISRSGNATPQPGDLVGT
                     SGIVKPGARDVKIVVDKVLP"
     gene            complement(1679..>1966)
                     /locus_tag="VF309_08020"
     CDS             complement(1679..>1966)
                     /locus_tag="VF309_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466572.1"
                     /translation="SLRAAANDGPDGCEGSGQMIPFTKTAADRAAAGDPRPSVAERYG
                     SFSGYYYARLKAVNDMMKEGLLLSEDAFAEFTRGLVSAQADYSLKNEQTDE"
BASE COUNT          295 a          681 c          708 g          282 t
ORIGIN      
        1 gagcgcttcg cgcaggtttt ccagcgccgt gaggtcgtcc ttgattcccg cggcgtgccc
       61 cgcgtagagg agttgcgcgc ggaacgggcc cggtgcgtcg cgcgcgtcgc gcccatccca
      121 gagcacttcg cccgcgtcgc agcgcgtgag acccgccgcg cagcgcagga gcgtcgtctt
      181 gccgctgccg ttgtgtccgc gcaccgcgac ccattctccg ggcgcgatcg cgaaggacac
      241 atcgcgaaac aggcggatgc cgccccgcga gcaggcgagg ccgcgcactt cgagggcgct
      301 caaggaagca ccttgtcgac cacgatcttc acgtcgcgcg cgcccggctt cacgatgccg
      361 ctggtcccta cgaggtcgcc cggctgcggc gtcgcgttgc cggagcgcga gatgcgcgcc
      421 tcgatgcgca ccgccggcgc gccggatatc ttcgtggccg gagtcatcgc catggagtcg
      481 tcgagcgcga agctcatggg aagctcgctc gccgaacggc gcagcaccgc gagcggcatg
      541 cgtgccccgc cttcggcgcg cgcgaagatg aagagggtgg cggcgttcga aacctgcgcg
      601 gtcagcggcg gcgcgacgct gacggagccc gacacggagc ccggaaccga tgccggaacc
      661 ggcgccttgg caatgcgctc cgccggcagg accttgccgg cagccgtggc cttcgtgcgc
      721 agctcgccga tgatcgcgcg cacctggcgc tcgtcatcgg agcccggcgg caattgcgcc
      781 gcaagggcct gccagtgacc gagcgcggcg gcgaagtcgt tctcgtccat cgccgcggtg
      841 ccggcgagcg cgagggcttt cttgtgaagg ggatcgatcg acagtgcttc cttcgcgatc
      901 tcgtagggac ggcccgcgag cgtgcgaccc tgcgccattc ccagcacatc ggcgtaatcg
      961 gcgagggtct gcgggtcacg cggcacgagc gtcaggagat gctcgtatgc gtcgaccgcc
     1021 tccttgaagc ggccgagcgc ggccatcgag cgcgcgagga gcgcccagcc ctgcgcgtcg
     1081 tcggggcgct tgcgcacctt cgccgcgagg ctttcgacca tcgcgaggat ctgcttgtcg
     1141 tcgagattgc cgccatcgtg ggcgatcgcg cccgtgtcga gcgccgacgg cgtgccgagc
     1201 gcgaggtaga tgccgaacgc cgcggcggga agcgccaccg cgacgacgac cgccgcggtc
     1261 caggacttcc tcgcgtcgcc gggcaccgcg gcgggaatgg ccgcgaggtc gtcctgcgcg
     1321 cgatcgacga gctcggcgag cgcctcgtcg cgggagctgg cgggcagtag cccgttggcg
     1381 acatcggcgt cgatctcgcg gcgctggccg cgcagcacgt cgaggttcgc cgcgacggcg
     1441 gtcggacccg cgcgcgagcg cgcgcgcagc agcggcacga gcacaaacga gagcgccacg
     1501 gctgtcatca gcgcggccat gatccagaag actgacattg ggaagagctg tgactcggag
     1561 tgagctgaaa ttataccgaa gccgccccgc ctgcttgacg tggaacaaat ggggggcggg
     1621 caaaaaagaa aggccccggc cgaagccggg gcccgcgagc gaggcgccgc ctcgcgaatc
     1681 actcgtccgt ctgttcgttc ttcaacgagt agtcggcctg ggccgaaacg aggcctcgcg
     1741 tgaattccgc gaacgcgtcc tcggacagca gcaggccctc cttcatcatg tcgttcaccg
     1801 ccttcaggcg cgcgtagtag tagccggaga agctgccata gcgctccgcg accgaggggc
     1861 ggggatctcc cgccgcggcg cgatccgcgg ccgtcttggt gaacgggatc atctggccgg
     1921 agccttcgca tccgtccggg ccgtcgttcg ccgcggcgcg cagcga
//
LOCUS       DASVII010000615         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_207948,
            whole genome shotgun sequence.
ACCESSION   DASVII010000615 DASVII010000000
VERSION     DASVII010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207948"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VF309_08025"
     CDS             complement(<1..290)
                     /locus_tag="VF309_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466573.1"
                     /translation="MTGIQRWGLAVLVTLLAAIAIVGGGVYWIVATPGGAQLVLSRAA
                     GILGKGTKIEGVEGSLGGVLRVKSILIDRPELYVRVDDLEMDSSTPFGGVLVV"
     gene            complement(293..>1764)
                     /locus_tag="VF309_08030"
     CDS             complement(293..>1764)
                     /locus_tag="VF309_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7466574.1"
                     /translation="TVTPGPRTVVETVDILFKGDIAGEGAAREKRRRDLIEGWTLPRG
                     RPFRSPEWDVAKTRLEEAATEIDYATATLVETRSEVDAEAAKAHLKIVVDSGPPFTLG
                     DVEIAGIDRYSEAVVRRLIDIERGERYSRARLLAVQRLVQSGPWFSSVVVDIEHDPMK
                     PNLAPVTVSVTERPARDVGLSVGYGTDDGFRGEAAYRDRDLFHRGLDLQSSVRVSQER
                     QIGYVDVYMPPGLARMRKLGDVPFTDSVGVLAEHNDTQNLGLSRFAVAGYRHFKLENV
                     ELRVGLSYQVERTYPEGSDISIKRALAPIVAVTWRHVDNVFDPRKGGVLNVQFAAGSK
                     SLGSGDDFFKAYVQYQYWIPLGAMDQLLLRTELGRTFAVSRDNIPEDFLYRAGGARSN
                     RGYAYQSLGVQEGNAIVGGRFLATGTVEYVHWLNERWGWALFTDIGGAADSPGDLQGL
                     KSYGVGARFKTPAGPLALDLAYAGRDHKFRLAFSVTIAF"
BASE COUNT          276 a          612 c          603 g          273 t
ORIGIN      
        1 acgacgagga cgccgccgaa cggcgtcgag gaatccatct cgaggtcgtc gacgcgcaca
       61 tagagctcgg ggcggtcgat gaggatcgat ttcacgcgca gcacgccgcc gaggcttccc
      121 tcgacgccct cgatcttcgt ccccttgccg aggattccgg cggcgcggga gagcacgagc
      181 tgcgcgccgc cgggcgtggc cacgatccag tagacgccgc cgcccacgat cgcgatcgcc
      241 gccagcagcg tgacgagaac ggcgaggccc cagcgttgga ttccggtcat ggtcagaacg
      301 cgatggtgac cgagaaggcg aggcggaact tgtggtcgcg ccccgcgtac gccaggtcga
      361 gcgccaacgg gcccgccggc gtcttgaagc gcgcgcccac gccgtagctc ttcaagccct
      421 gcaagtcgcc gggagaatcc gcggcgccgc cgatgtcggt gaagagcgcc cagccccagc
      481 gctcgttcag ccagtgcacg tactcgacgg tgccggtggc gaggaagcgc ccgccgacga
      541 tcgcgttgcc ttcctgcacg cccaggctct ggtaggcata gccgcggttc gagcgcgcgc
      601 ctcccgcgcg atagaggaag tcctcgggaa tgttgtcgcg gcttaccgcg aaggtgcggc
      661 cgagctcggt gcgcagcagg agctggtcca tggcgcccag cgggatccag tactggtact
      721 gcacgtacgc cttgaagaag tcgtcgccgg agccgaggga tttcgagccc gccgcgaact
      781 gcacgttgag cacgccgccc ttcctcggat cgaacacgtt gtccacgtgc cgccacgtca
      841 ccgcgacgat cggcgccagc gcgcgtttga tgctgatgtc ggagccttcc ggataggtgc
      901 gctcgacctg gtatgagagc cccacgcgca gctcgacatt ctcgagcttg aagtggcggt
      961 agccggcgac cgcgaagcgg ctcaagccca ggttctgggt gtcgttgtgc tcggcaagga
     1021 cgcccacgct gtccgtgaac ggcacgtcgc ccagcttgcg catcctggca aggcccggcg
     1081 gcatgtacac gtcgacgtag ccgatctgtc gctcctggct aacgcgcacc gagctctgca
     1141 ggtcgaggcc gcgatggaaa agatcgcgat cgcggtacgc ggcctccccg cggaatccgt
     1201 cgtccgtgcc gtagcccacc gaaagcccca cgtcacgcgc gggacgctcg gtgacgctca
     1261 cggtgaccgg cgccaggttg ggcttcatcg ggtcgtgctc gatgtccacc accacgctcg
     1321 agaaccacgg cccgctctgc accaggcgct gcacggcgag cagccgcgcg cggctgtagc
     1381 gctcgccgcg ctcgatgtcg atcagccggc gcaccaccgc ctcggaatag cgatcgatgc
     1441 cggcgatctc cacgtcgccc agcgtgaagg gcgggccgct gtccaccacg atcttcaggt
     1501 gcgccttggc ggcctcggcg tcgacctcgg agcgcgtttc gacaagcgtc gccgtggcgt
     1561 agtcgatctc ggtcgcggcc tcctccagac gcgtcttggc gacgtcccat tcggggctgc
     1621 ggaagggccg cccgcgcggg agagtccagc cttcgatgag atcgcggcgg cgcttctcgc
     1681 gggccgcgcc ctcgccggcg atgtcgccct tgaacaggat gtcgacggtt tccacgacgg
     1741 tgcgcggtcc aggcgtgacg gtga
//
LOCUS       DASVII010000616         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_64774, whole
            genome shotgun sequence.
ACCESSION   DASVII010000616 DASVII010000000
VERSION     DASVII010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64774"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VF309_08035"
     CDS             <1..1523
                     /locus_tag="VF309_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEX7466575.1"
                     /translation="AHIHGGLQVDTPDPAFNVLANGWLLYQTLACRLWARSGYYQSGG
                     AFGFRDQLQDVMALLHTEPTLVREHLLRAAGRQFVQGDVQHWWHPPSGRGVRTRCSDD
                     YLWLPLATARYVKATGDNAVLDESVPFLEARAVNPGDDSYYDLPIRSSESASLYQHCV
                     RAVTNGLRYGAHGLPLMGSGDWNDGMNLVGIQGAGESVWLGFFLCSVLEQFAPVARAK
                     GDADFAAQCDTQRADLGRNLERHGWDGAWYRRAYFDDGTPLGSASNVECRIDSIAQSW
                     SVLSGAGDPARARMAMKALEEHLVRRDSGLVQLLDPPFDKSSLDPGYIRGYVPGVREN
                     GGQYTHGAIWAAMAFAALGDRERAWELATMMNPVTHSRTSRDVATYKIEPYVIAADVY
                     AMAPHVGRGGWSWYTGSAGWMYRLLIESLLGLSREKDRLCFAPCIPADWKEFAIRYRY
                     GATVYDIAVRQVGGAAGAAIVTENGVAQPDGRVRLVDDRAEHRVMVMLAGRQTGAG"
BASE COUNT          263 a          567 c          586 g          277 t
ORIGIN      
        1 gggcccatat ccatggcggc ctgcaggtcg atacacccga tccggctttc aatgtgctgg
       61 ccaacggttg gctgctatat caaaccctgg cctgtcgcct gtgggcgcgc agcggctatt
      121 accagtcggg cggggccttc ggcttccgcg accagctgca ggacgtgatg gcgctgttgc
      181 acaccgagcc aacgctcgtg cgcgagcacc tgctgcgcgc cgccggccgc cagttcgtgc
      241 agggcgacgt gcagcactgg tggcatccgc cgtccggacg cggagtgcgc acgcgttgct
      301 cggacgatta cctgtggctg ccgctcgcca cggcgcgcta cgtgaaagcc accggcgaca
      361 acgccgtgct cgacgaatcg gtcccgttcc tcgaggcccg ggcggtgaat ccgggcgacg
      421 attcctacta cgacctgccg atccgctctt cggaatcggc gagcctctac cagcactgcg
      481 tgcgcgccgt cacgaacggg ctgcgctatg gcgcgcacgg gctgccgctg atgggctccg
      541 gcgactggaa cgacggcatg aacctggtcg ggatccaggg tgccggcgaa agcgtctggc
      601 tgggattttt cctgtgcagc gtgctggagc aattcgcacc ggtggcgcgc gcgaaaggcg
      661 acgccgactt tgccgcgcaa tgcgataccc agcgcgccga tctcggccgc aacctcgagc
      721 gccacggctg ggacggcgcg tggtaccggc gcgcctattt cgatgacggc acgccgctag
      781 gatcggcgag caacgtcgag tgccgcatcg attccatcgc gcaaagctgg tcggtgttgt
      841 ccggggcggg ggatcccgcg cgcgcgcgca tggcgatgaa ggcgctcgag gagcatctcg
      901 tgcgccgcga ctcgggattg gtgcagctcc tcgatccgcc gttcgacaag tcctccctcg
      961 atcccggcta catccgcggg tatgtgccgg gcgtgcgcga gaacggcggc cagtacacgc
     1021 acggcgccat ctgggcggcg atggcgttcg cggccctcgg cgatcgcgag cgcgcatggg
     1081 agctcgcgac gatgatgaac ccggtgaccc actcgcgcac gtcccgggat gtcgcgacct
     1141 acaagatcga gccctacgtg atcgccgccg acgtctacgc gatggcgccc cacgtcgggc
     1201 gcggcggatg gagctggtac acgggatcgg cgggctggat gtatcggctc ctcatcgagt
     1261 cgctcctcgg gctctcgcgc gagaaggacc ggctatgctt cgcgccgtgc attcccgcgg
     1321 actggaagga attcgcgatc cgctacaggt acggcgcaac ggtgtacgac atcgccgtgc
     1381 gccaggtggg cggcgccgcg ggtgcggcga tcgtgaccga gaacggcgtt gcgcaacccg
     1441 acggccgcgt gcggctggtc gacgatcgcg ccgagcatcg cgtgatggta atgttggctg
     1501 gcagacagac cggcgcgggc taggcccgca ggatcgcacg tccatggcct ctcccaaacc
     1561 catccagatc atcctggcgc ggcaattcgc ggcgagcctc gccatgccca tcctcatcgt
     1621 ggacaccgag gggacgctca tcttcttcaa tgagccggcg gaggtgatcc tcggccagcg
     1681 cttcgaggag acc
//
LOCUS       DASVII010000617         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_29164, whole
            genome shotgun sequence.
ACCESSION   DASVII010000617 DASVII010000000
VERSION     DASVII010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29164"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VF309_08040"
     CDS             <1..392
                     /locus_tag="VF309_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466576.1"
                     /translation="IGMIILGLGFVVLAIADSRAQAVGQVGPWWLVAVYMLHTVGELC
                     LSPVGLSMVTKLAPARVAALMMGIWYLANAAANYLAGILEDLLKGSSMPLYWFLVGSS
                     IGAGIVLLAITPLVKRLMHEGHSAKKG"
     gene            complement(395..868)
                     /locus_tag="VF309_08045"
     CDS             complement(395..868)
                     /locus_tag="VF309_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466577.1"
                     /translation="MLLISLRFLALMLAALTAGMSFAHVLERPAKMRYDAALYGTLQK
                     TLYAYWGPPNVGAFVEIMAIAATLMLAWLVSDRGSALWMTAGAATALVIAFPIVFFTL
                     VAPANKAFLEASPPAMPADWMEMRARWEDGHVIRFAFHLCALGLLILSVLIEMDA"
     gene            complement(1006..>2287)
                     /locus_tag="VF309_08050"
     CDS             complement(1006..>2287)
                     /locus_tag="VF309_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7466578.1"
                     /translation="RNPLQVLPRAVYEEDFVAFGGAKQPRAWVTSPELVKAVLLDERE
                     KFGKLTQIRLLGPLLGKGILTSEGADWKWQRQAAAPMFRPQELASFVPAFVRAAEDAL
                     ARWRAMPPGSVQAIDEDMTRATFEVIAATLLPSADENFARTVQGSVQSLQRFGPWGIL
                     YASMNLPPWLPHPGMFARARAVRTLRSTVTALVRERKGESDPPDDLMRRLIAARDPET
                     GRSMGEAQLVDNLLTFYLAGHETTAKALTWTLYLLARSPEWTAALEDEIERVTGGAAI
                     RGDQAADLVKVQQVLKESMRLYPPVPMMSRQAVADAQIDGHAIRAGTSILMPIYAIHR
                     HAKRWDRPDEFDPARFSPEREAAIPRYQYMPFGAGPRLCIGMPFAMIEATVILATFLQ
                     RARFALARDEEPTPVASVTLIPKGGMPLKVSMKG"
BASE COUNT          361 a          741 c          823 g          362 t
ORIGIN      
        1 cgatcggcat gatcatcctc gggctcggat tcgtcgtgct cgcgatcgct gactcccgcg
       61 cgcaagccgt cgggcaggtg gggccgtggt ggctggtagc ggtgtacatg ctgcataccg
      121 tgggcgagct ttgcctctcg cccgtggggc tgtcgatggt gaccaagctc gctcccgcgc
      181 gcgtcgcggc gctcatgatg gggatctggt acctcgccaa cgccgcggcg aactacctcg
      241 cgggaatcct cgaggatctg ctgaagggct cgtcgatgcc gctctactgg ttcctcgtcg
      301 gcagctcgat cggcgcgggg attgtgctcc tggcgatcac gccgctggtg aagcggctga
      361 tgcatgaagg acactcggcg aagaagggct gacgtcaggc atccatctcg atcagcaccg
      421 agaggatgag aagcccgagg gcgcacagat ggaacgcgaa gcgaatcacg tggccgtctt
      481 cccagcgcgc gcgcatctcc atccagtcgg cgggcatggc gggggggctg gcctcgagaa
      541 aggccttgtt ggcgggcgcg acgagcgtga agaacacgat cgggaaagcg atgacgaggg
      601 cagtcgcggc gccggccgtc atccataggg cggacccccg gtcgctcacc agccacgcga
      661 gcatgagcgt cgcggcgatc gccatgatct cgacgaaagc gccgacattg ggcggtcccc
      721 agtacgcgta cagggttttc tggagcgtgc cgtagagcgc cgcgtcgtag cgcatcttgg
      781 cggggcgctc gaggacgtgc gcgaacgaca ttcccgcggt gagcgccgcg agcatgaggg
      841 ccaggaaacg cagggagatg agcaacatcg tcccaggtac ggtatcgcac cgtcgcgccc
      901 gaggcgtgtg aatttgtaac cggatgcttg cccgtgccga cgagaggccg ggcgcgatga
      961 aatggattgt tgcagaagcc cggatcgagg cggcgccgcg gcccgctagc ccttcatcga
     1021 caccttcagc ggcattccgc ccttcgggat gagcgtcacg ctcgcgacgg gcgtgggctc
     1081 ctcgtcgcgg gcgagcgcga agcgcgcgcg ctgcaggaag gtcgcgagga tcaccgtggc
     1141 ctcgatcatc gcgaagggca tgccgatgca caggcgcggc ccagccccga agggcatgta
     1201 ctggtagcgg ggaatcgccg cctccctttc gggggagaag cgcgccggat cgaattcgtc
     1261 cgggcgatcc cagcgcttcg cgtggcgatg gatggcgtag atcggcatga ggatcgacgt
     1321 cccggcgcga atggcatggc cgtcgatctg cgcatcggcc acggcctggc ggctcatcat
     1381 cggcacgggt ggatagaggc gcatcgattc cttgagcacc tgctgcacct tcacgagatc
     1441 ggcggcctgg tcgccacgaa tcgcggcgcc gcccgtcacg cgctcgatct cgtcttcgag
     1501 cgccgcggtc cactcgggcg atcgcgccag gaggtacagg gtccacgtga gcgccttcgc
     1561 cgtggtctcg tggcccgcga ggtagaacgt gagcaggttg tcgacgagct gcgcctcgcc
     1621 catcgagcgg ccggtctcgg gatcgcgcgc ggcgatgagg cgccgcatga ggtcgtcggg
     1681 cggatcgctc tcgcccttgc gctcgcgcac cagcgccgtg accgtcgagc gcagcgtcct
     1741 tactgcgcgc gcccgcgcga acatgccggg gtggggcagc cagggcggca ggttcatcga
     1801 ggcgtagagg attccccagg ggccgaatcg ctgcagcgac tgcaccgagc cctggacggt
     1861 gcgggcgaaa ttttcgtcgg ccgacggcag cagcgtcgcc gcgatcactt caaaggtcgc
     1921 gcgtgtcatg tcctcgtcga tggcctgcac cgagcccggc ggcatcgcgc gccaccttgc
     1981 gagcgcatcc tccgcggcgc gcacgaacgc cgggacgaag ctcgccagct cctgcgggcg
     2041 gaacatcggc gcggccgcct ggcgctgcca cttccagtcc gcgccttcgc tcgtgaggat
     2101 gcccttgccg aggagcggcc cgaggaggcg gatctgcgtg agcttgccga acttctcgcg
     2161 ctcgtcgagg agcaccgcct tgacgagctc gggagaggtc acccatgcgc gcggctgctt
     2221 cgcgccgccg aaggcgacga agtcttcctc gtagaccgcg cgcggcagaa cctggagcgg
     2281 gttgcgc
//
LOCUS       DASVII010000618         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_172152,
            whole genome shotgun sequence.
ACCESSION   DASVII010000618 DASVII010000000
VERSION     DASVII010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172152"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..>3302)
                     /locus_tag="VF309_08055"
     CDS             complement(15..>3302)
                     /locus_tag="VF309_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEX7466579.1"
                     /translation="PGLAGRIDLRADVKLTRDGARWRAEGDAYGESRNADLGLLRAHL
                     PVPDSLRSGVGSVRVWAHFNRDGVNEVVADLNMRDAKAQLAADLLPLELATISGRATY
                     RAEVDGFTFATQGLRFQLASGVVARVGDFSLSRRLEAGKPERVAISADGIDLKIAATL
                     LDYFPVPRDVKGQVLRFAPRGRILDATVAWTGGAGAPVLAYTVKGRFEDLAINPVEQY
                     PGVSGITGTIDGTQDGGSVQIESKNVGFVLERFFKAPLAFDAIDAHATWKHVGNAIEV
                     AIADARFANPDAEGQVSGTWRSLPDAKERSPGFVDLKGTFSRALSSRVAYYMPNRIAA
                     TRDWLERAIQAGTSSHIDFEVKGDLWGFPFGPDQPGHFRVQGDVRDGRIKYHPDWPSV
                     DAIRGTFKFENRRMEIHADQATIFASRAAGVTAIVDDLAAKPPVLTLNADIDTTGADT
                     VRFLRESPLVNGPGSFTRAVAIEGPGRLKLQLVYPMWGVDPVRVTGDYLFSGATATVG
                     RSLAMRDVRGHLAFTERGVRANDVVGTLFGKPASLTIATQPDGQVVTQIEGRLDASAM
                     GAYAPEAIVTRLGGGADWKARVVSGKQGSELVVTSDLKGLESRLPPPLAKAADDPRPA
                     TFTMSKLGTENEVTTVALSGGVNARISRSVVAGAERWNAALKFGAPVGSEPVREGLWL
                     YGELPFLDVDAWQAIFAMPRGGDAPPAAPPGVELRGLELKLARVRYWGREFAQMAAQL
                     ARGGSEWSGKLESPLVAGDIRWNWEGKGRLAAKLERLSIIEPTPVAGAVEAQRVDSDL
                     PALDVTAERFEFKGKWLGRLDLKAEPDGDEWRIDKLDIVNGHANFRSTGAWRRTAAGS
                     LTSLHLKLETENLNALMGQFGYGDYLKRGSGALEGALVWPGYPYDFSTSILAGTLRVE
                     AQKGQFAKIEPGAGKLLGLLSLQSLPQRAMFDFRDVFSAGFAFDRIHGDVKITRGVLL
                     TDGFEISGPAAFVSMSGEVSLPQETQSLVMHIVPEVGEGVALAATLIGTPVLGLSTLL
                     VSKLLKNPLGNVVAYEYQITGSWDNPQVTRLSAPPPKTAAAPPNAAPSVPTTTAANPP
                     VEPARNP"
BASE COUNT          483 a         1151 c         1169 g          499 t
ORIGIN      
        1 ggtgatgatc atcgtcaggg attacgcgcc ggttcgacgg gcggattcgc cgcggtcgtc
       61 gtgggaacgg atggcgcggc gttggggggt gccgcggcgg tcttgggagg cggcgccgag
      121 agtcgcgtga cctggggatt atcccacgag cccgtgatct ggtactcgta cgcgaccacg
      181 ttgccgagcg ggttcttcag cagcttggag acgaggagcg tcgagagccc gagcaccggc
      241 gtgccgatga gggtcgccgc cagcgcgacg ccttcgccca cttcggggac gatgtgcatc
      301 accagggact gcgtctcctg cggaagggac acctcgcccg acatgctcac gaacgcggcg
      361 ggtccggata tctcgaagcc atcggtgagg agcacgccgc gcgtgatctt cacatcgccg
      421 tggatgcggt cgaaggcgaa gccggcgctg aacacgtcgc ggaaatcgaa catcgcgcgc
      481 tgcggcagcg attgcagcga cagcagcccc aggagcttgc ccgcgcccgg ctcgatcttc
      541 gcgaactgcc ccttctgcgc ctcgaccctg agcgtgcccg cgaggatcga cgtggagaaa
      601 tcgtagggat agcccggcca caccagcgcg ccttcgagag cgccgctgcc gcgcttcagg
      661 tagtccccgt agccgaactg ccccatgagc gcgttcaggt tctcggtctc gagcttcagg
      721 tgcagcgacg tgagcgagcc ggcggccgtg cggcgccaag cgccggtcga gcggaaattc
      781 gcgtgcccgt tcacgatgtc gagcttgtcg atgcgccact cgtcgccgtc gggctcggcc
      841 ttgaggtcga ggcggccgag ccacttgccc ttgaactcga aacgctccgc ggtgacgtcg
      901 agggcgggaa ggtcggagtc gacgcgctgc gcttcgaccg cgccggcaac cggcgtgggc
      961 tcgatgatgg agaggcgctc gagcttcgcg gccaggcgtc ccttgccttc ccagttccag
     1021 cggatgtcgc ccgcgacaag ggggctctcg agcttgccgg accactcgga gccgccgcgg
     1081 gcgagctgcg cggccatctg cgcgaactcg cgcccccagt agcgcacgcg ggcgagcttg
     1141 agctcgaggc cgcgcagctc cacgcccgga ggcgccgccg gcggcgcgtc gccgccccgc
     1201 ggcatggcga agatcgcctg ccacgcatcc acgtcgagaa acgggagctc gccgtagagc
     1261 cagaggccct cgcgcaccgg ctccgatccc acgggagcgc cgaacttgag ggcggcgttc
     1321 caacgctcgg ctccggcgac gacgctcctc gagatcctcg cgttcacgcc gccggagagc
     1381 gccaccgtcg tcacttcgtt ctccgtgccg agcttcgaca tcgtgaaggt cgcgggccgc
     1441 gggtcgtcgg cggccttcgc gagcggcggc ggaaggcgcg actcgagccc cttcaggtcc
     1501 gaggtgacca cgagctcgct tccctgcttg cccgacacga cgcgcgcttt ccagtccgcg
     1561 cctccgccga gcctcgtgac gatcgcctcg ggcgcgtagg cgcccatcgc gctcgcgtcg
     1621 agcctgcctt cgatctgcgt gacgacctgg ccatccggct gtgtcgcgat cgtgagcgac
     1681 gcgggcttgc cgaagagggt gcccacgacg tcgttcgcgc gcacgccgcg ctccgtgaac
     1741 gccaggtggc cgcgcacgtc gcgcatcgcg agcgaccggc ccacggtcgc cgtggcgccg
     1801 gagaagaggt agtcgccggt caccctcacc ggatccacgc cccacatcgg gtacacgagc
     1861 tgcagcttga ggcgccccgg cccctcgatg gcgaccgcgc gcgtgaacga gccgggcccg
     1921 ttcaccagcg gagactcgcg aaggaagcgc accgtgtcgg ctcccgtggt gtcgatgtcg
     1981 gcgttcagcg tgagcaccgg cggtttcgcg gcgagatcgt cgacgatcgc ggtcacgccg
     2041 gcggcgcggc tcgcgaagat ggtcgcctgg tccgcatgga tctccatgcg gcgattctcg
     2101 aacttgaagg tgccgcggat cgcatccacc gacggccagt cggggtggta cttgatgcgc
     2161 ccgtcgcgca cgtcgccctg gacgcggaaa tggcccggct ggtcggggcc gaacgggaag
     2221 ccccacaggt cgcccttcac ctcgaagtcg atgtggctgc tggttcccgc ctggatcgcg
     2281 cgctcgagcc agtcgcgcgt cgcggcgatg cgattgggca tgtagtaggc gacgcggctc
     2341 gacagcgcgc gggagaacgt gcccttgaga tcgacgaatc ccggcgagcg ctccttcgcg
     2401 tcgggaaggg agcgccacgt gccggagacc tggccttcgg cgtccgggtt ggcgaagcgc
     2461 gcatcggcga tcgcgacctc gatcgcgttg ccgacgtgct tccacgtcgc gtgggcgtcg
     2521 atcgcgtcga acgcgagcgg cgccttgaag aagcgctcga gcacgaagcc gacgttcttc
     2581 gactcgatct ggacgctgcc gccgtcctgc gttccatcga tcgtgcccgt gattcccgag
     2641 accccagggt attgctccac gggattgatg gcgagatcct cgaaccggcc cttcaccgtg
     2701 tacgccagca cgggcgcgcc ggcgcctccc gtccacgcga ccgtggcgtc gaggatgcgg
     2761 ccgcggggcg cgaagcgcag cacctggcct ttcacgtcgc gcggcaccgg gaagtaatcg
     2821 aggagcgtcg ccgcgatctt caggtcgatg ccgtcggcgc tgatcgcgac ccgctcgggc
     2881 ttgcccgcct cgaggcggcg cgagagggag aaatcgccga cgcgcgcgac gacgccgctg
     2941 gcgagctgga atcgcagccc ctgggtcgcg aacgtgaacc cgtcgacctc cgcgcggtag
     3001 gtcgcgcgcc ccgagatcgt cgcgagctcg agcggcagca agtcggcggc gagctgcgcc
     3061 ttcgcgtcgc gcatgttgag gtccgcgacg acctcgttca cgccgtcgcg attgaaatgc
     3121 gcccacacgc gcacgcttcc cacgccgctt cgcagggaat ccgggaccgg aaggtgcgcg
     3181 cgcagcaatc cgagatcggc gttgcggctc tcgccgtagg catcgccctc ggcgcgccac
     3241 cgcgcgccat cgcgcgtgag cttcacgtcg gcgcgaaggt cgatgcgccc ggcgagcccg
     3301 gg
//
LOCUS       DASVII010000619         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_29328, whole
            genome shotgun sequence.
ACCESSION   DASVII010000619 DASVII010000000
VERSION     DASVII010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29328"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1723
                     /gene="ipdC"
                     /locus_tag="VF309_08060"
     CDS             83..1723
                     /gene="ipdC"
                     /locus_tag="VF309_08060"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR03394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HEX7466580.1"
                     /translation="MPIKTTVGGVLIEALHAHGAREIFGIPGDFALPFFDAIERMPIL
                     PLYTLSNEPSLGFAADACARMRGAPAVVAVTYGAGALNVVNPIACAYAEKSPVVVISG
                     APGRAARASGLLVHHEARRPESQFNIYSEITCDQARLDDPATAAASIARVLSSARRWA
                     RPVYIELPSDVVDAPSASVTPLPAIAPDAEALEACANEILERLARAKQPVLMVGVEVR
                     RFGLEERVARLARTLRIPVVTSFLGRGLLTDTDAPLVGTYLGLAGDPEVSALVEDSDG
                     LFLLGEILSDTNFGVSRGRIDMRHTIQALDTHVTLSHHTYPDIPLDALVASLERIARP
                     LASAKRVEPRAPPHGMPHDAAPIVPLDVAAAINDLMEAHGRYPVAADIGDCLFTAMDL
                     VHTDHVAPGYYATMGFAVPAAIGVQVATGRRPLAIVGDGAFQMTGMELGHCRRYGLDP
                     IVVVMNNASWGMLSAFRPDARYTDIGEWDFAALANPLGGRGYRVSTRAQLAQALEEAA
                     KNRGVFQLIDARIARDALSPTMRRFAAAMGGKRPAAGG"
BASE COUNT          243 a          638 c          601 g          263 t
ORIGIN      
        1 ggctcgacgc ggcgagcggg gaggcggtgc tggtggggct tcgggacgcg aagttcgggg
       61 cgctgaacta ggcgaccttg acatgcccat caagaccacc gtgggtggcg ttctcatcga
      121 ggcgctccac gcccacggcg cccgcgagat cttcggcatc ccgggcgatt tcgcgctgcc
      181 gttcttcgac gcgatcgagc gcatgccgat ccttccgctc tacacgctct ccaacgagcc
      241 ctcgctcggc ttcgcggcgg acgcgtgcgc gcgcatgcgc ggcgcacccg cggtggtcgc
      301 cgtcacgtat ggcgcgggtg cgctcaacgt cgtcaatccc atcgcctgcg cctatgccga
      361 gaagtcgccc gtggtggtga tctccggcgc gccgggacgc gcggcgcgcg cgagcgggct
      421 cctcgtgcac cacgaggcgc ggcggcccga atcgcagttc aacatctaca gcgagatcac
      481 ctgcgaccag gcgcgcctcg acgaccccgc gaccgctgcc gcatcgatcg cgcgcgtgct
      541 ctcgagcgcg cggcgttggg cgcgtccggt ttacatcgag cttccgagcg acgtcgtcga
      601 tgcgccctcg gcatcggtga cgccgctgcc cgccatcgcg cccgacgcgg aagcgctcga
      661 ggcatgcgcg aatgaaatcc tcgagcgcct cgcgcgggcg aagcagccgg tgctgatggt
      721 gggcgtcgag gtgcgccgct tcggcctcga ggagcgcgtg gcgcggctcg cgcgcacgct
      781 gcgcatcccc gtggtgacga gcttcctcgg gcgcgggctt ctcaccgaca ccgacgcgcc
      841 tctcgtggga acgtatctcg gactcgcggg cgatccggag gtgagcgcgc tcgtggagga
      901 ttccgacggc ctcttcctcc tcggcgagat cctctcggac acgaacttcg gcgtatcgcg
      961 cgggcgcatc gacatgaggc acacgatcca ggcgctcgac acgcacgtca ccctctcgca
     1021 ccacacctat cccgacatcc ccctggacgc gctggtcgcc tcgctcgaac ggattgcgcg
     1081 cccgctcgca tcggccaagc gggtggagcc gcgagcgccg ccccatggga tgccgcacga
     1141 cgcagcgccg atcgtgccgc tcgacgtggc cgcggcgatc aacgacctca tggaagccca
     1201 cggccgctac ccggtggcgg ccgacatcgg cgattgcctc ttcaccgcga tggacctcgt
     1261 gcacaccgac catgtcgcgc ccggctatta cgcgaccatg ggattcgccg ttcccgccgc
     1321 gatcggtgtc caggtcgcga ccggacggcg cccgctcgcg atcgtcggcg acggcgcgtt
     1381 ccagatgacg ggcatggagc tcggccactg ccggcgctac ggcctcgacc cgatcgtggt
     1441 cgtgatgaac aacgcgagct ggggaatgct ctcggcgttc cgtcccgacg cgcgctacac
     1501 ggatatcggc gagtgggact tcgcggcgct cgcgaacccg ctcggagggc gcggctatcg
     1561 cgtcagcacg cgcgcgcagc tcgcgcaggc gctcgaggag gccgcgaaga atcgcggcgt
     1621 cttccagctc atcgacgcgc gcatcgcgcg cgacgcgctc tcgcccacga tgcggcgctt
     1681 cgccgcggcg atgggcggca agcgccccgc agcgggcggc tagtccagcg cgtacaggtt
     1741 gccgt
//
LOCUS       DASVII010000620         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_208239,
            whole genome shotgun sequence.
ACCESSION   DASVII010000620 DASVII010000000
VERSION     DASVII010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208239"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VF309_08065"
     CDS             <1..605
                     /locus_tag="VF309_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466581.1"
                     /translation="FETAPPNDGRFEGPRRFALTPLTEMTPEQRAVADTIMKGPRVAV
                     GSPATAAGATSLASPFNVWNRRPELADRLQRIGEYIRFKSSLPPRLNEFAILIVAREW
                     NSQYEWFAHHRLAMAGGLDPAVAEDLAQGRVPASMKPDEQVVYDFSMEMHRRHGVSDA
                     TFKRAVDALGEQGVADLIAVNGYYVLVAMTVNVDRTPIPR"
     gene            complement(602..1006)
                     /locus_tag="VF309_08070"
     CDS             complement(602..1006)
                     /locus_tag="VF309_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466582.1"
                     /translation="MRFSTGIGRRMDKKRRPIEWFDLCEEHNYPAALSYLTLLFTPKE
                     ARACVKRLRAAQVSKFKAKDIFRASGLSLLGVSNSHVVKDRKKILAGTPLSPLLLVRR
                     AGRAALIVADGYHRLCAVYAFNEDAVIPCKIV"
     gene            1045..1524
                     /locus_tag="VF309_08075"
     CDS             1045..1524
                     /locus_tag="VF309_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX7466583.1"
                     /translation="MQGLDDAAWIAARLAAALFVGAVVGINRDLHRKAAGLRTLALVA
                     VGSAVLVIVTIIMTDGNGDSLSRVIQGIVAGVGFLGAGVIMHHETGGRVEGLTTAASV
                     WVAAGLGAACGAGLMVVAILALLTTMAILTFGGRVERALERRFWKPGTETRPEDQEH"
     gene            complement(1544..2191)
                     /locus_tag="VF309_08080"
     CDS             complement(1544..2191)
                     /locus_tag="VF309_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466584.1"
                     /translation="MNRRTACALLAALSATFAIDSMAADPPAGEKRFPLLTPGELSEQ
                     QKPLGEQILKVSSVGLGGPYNPMLRSPVLGQRMFDLLYYLRWNTSVPLRLNEFAILII
                     GRQWRSQVEWFAHAPLAIKSGLSPEIVAELKTNKRPSNMKPDEAIVYDFVTELTAKHA
                     VSDETFKRAKEILGEQQVVDLTAVSGTYVTVAMILAMAEEGVPAGKELPFKPGEP"
     gene            2255..>2474
                     /locus_tag="VF309_08085"
     CDS             2255..>2474
                     /locus_tag="VF309_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7466585.1"
                     /translation="MANRRQFLQGTAAGLCFVGCGIAGHAHAQAPARKREVVVNGKRA
                     RTIDVHAHCAIPAALAVASDAKLELPATT"
BASE COUNT          403 a          831 c          847 g          393 t
ORIGIN      
        1 acttcgagac cgcgcccccg aacgacgggc gcttcgaagg cccgcggcgc ttcgcgctca
       61 cgcctctcac cgagatgacg cccgagcagc gcgccgtcgc cgacaccatc atgaaaggtc
      121 cgcgcgtggc ggtcggcagc ccggcgacgg cggcgggagc gacgagcctc gcgagcccgt
      181 tcaacgtgtg gaaccgcagg cccgagctgg ccgaccggct gcaaaggata ggcgaataca
      241 tccgcttcaa gagctcgctg ccgccgcggc tgaacgagtt cgcgatcctc atcgtcgcgc
      301 gcgagtggaa ttcgcagtac gaatggttcg cccaccaccg cctcgcgatg gccggcgggc
      361 tggatcccgc cgtcgccgag gatctcgcgc aaggacgcgt gccggcgagc atgaagcccg
      421 acgagcaggt cgtctacgac ttcagcatgg agatgcaccg gcggcatggc gtgagcgacg
      481 cgaccttcaa gcgcgccgtc gacgcgctgg gcgagcaggg agtcgcggac ctcatcgccg
      541 tgaacggcta ctacgtgctg gtcgcgatga ccgtgaacgt cgaccgcacg ccgatcccgc
      601 gctagacgat cttgcacggg atcacggcat cctcgttgaa cgcgtagacc gcgcagaggc
      661 ggtgatagcc gtcggccacg atcaacgcgg cgcgcccggc ccttcgcacc agcagcagcg
      721 gcgagagcgg ggtcccggcg aggatcttct ttcggtcctt caccacgtgg gagttgctca
      781 ctccgaggag cgacaggccc gaggcgcgga agatgtcctt cgccttgaac ttcgaaacct
      841 gcgccgcgcg caggcgcttg acgcaggccc tcgcttcctt cggcgtgaag aggagcgtca
      901 agtacgacag cgcggcgggg tagttatgct cctcgcacag gtcgaaccat tcgatgggcc
      961 gccgcttctt gtccatgcgc ctcccgatgc cggtggaaaa gcgcattgta gagtgatcgc
     1021 cttatgaatc cgtttggagg aatcatgcaa ggactcgacg acgcggcatg gatcgcggca
     1081 cggctggcgg cggcgctctt cgtgggcgcg gtcgtgggga tcaaccgcga cctgcatcgc
     1141 aaggccgcgg gcctgcgcac gctcgcgctg gtcgcggtcg gcagcgccgt cctggtgatc
     1201 gtcacgatca tcatgacgga cggcaacggc gattccttga gccgcgtcat ccaggggatc
     1261 gtggcgggcg tcggattcct cggcgccggc gtgatcatgc atcacgagac gggcgggcgc
     1321 gtcgagggcc tcaccacggc cgcttcggta tgggtcgcgg cgggcctcgg cgcggcctgc
     1381 ggcgccgggc tgatggtggt cgccatcctc gcgctcctca cgaccatggc catcctcacg
     1441 ttcggcggcc gcgtagagcg cgcgctcgag cgccggttct ggaaacccgg caccgagacg
     1501 cgccccgagg accaggagca ctaggcccgg cgctacgccc tggtcatggc tcgcccggct
     1561 tgaacggaag ctccttgccg gcgggcacgc cctcctccgc catcgcgagg atcatcgcca
     1621 ccgtcacgta ggttcccgac accgccgtga ggtccacgac ctgctgctcg ccgaggattt
     1681 ccttcgcgcg cttgaaggtc tcgtcggaga ccgcgtgctt cgccgtgagc tcggtcacga
     1741 aatcgtagac gatcgcctcg tcgggcttca tgttcgaggg ccgcttgttc gtcttgagct
     1801 cggccacgat ctcgggcgaa agccccgact tgatcgcaag cggcgcgtgg gcgaaccatt
     1861 caacctggga gcgccactgc cttccgatga tgaggatcgc gaactcgttc aggcgcagcg
     1921 gcaccgacgt gttccagcga aggtagtaga gaaggtcgaa catgcgctgc ccgaggacgg
     1981 ggcttcgcag catcgggttg taggggccgc cgagccccac gctcgacacc ttcaggatct
     2041 gctcgccgag gggcttctgc tgctccgaga gctccccggg cgtgagcagc gggaagcgct
     2101 tctcgcccgc cggcgggtca gcggccatgg aatcgatcgc gaacgtcgcg gacagggcgg
     2161 ccaatagcgc gcatgcggtt ctcctgttca tgtgggttct cctcccgggg tggacattgc
     2221 tatagtgcca ccacaactaa acggaggagg caccatggcg aaccgcaggc aattccttca
     2281 gggtacggcg gcggggctct gcttcgtggg ttgcggcatc gcggggcatg cgcacgcgca
     2341 ggcccccgcg cgcaagcgcg aggtcgtcgt gaacggcaag cgcgcccgca ccatcgacgt
     2401 ccatgcgcat tgcgcgattc cggcggccct cgcggtcgcg agcgacgcga aactcgagct
     2461 cccggcgacg acga
//
LOCUS       DASVII010000621         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_172260,
            whole genome shotgun sequence.
ACCESSION   DASVII010000621 DASVII010000000
VERSION     DASVII010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172260"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1227)
                     /locus_tag="VF309_08090"
     CDS             complement(34..1227)
                     /locus_tag="VF309_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7466586.1"
                     /translation="MPAGRHFLQIPGPTNVPDRVLRAIDRPTIDHRGPEFQKLGKEVL
                     EGIKRIFQTRNPVIIFPASGTGAWEAALVNTLSPGDRVLMCETGHFASLWQKMAKKLS
                     LEIDMIPGDWRHGVDAGEIEARLAADKAHAIKAVCVVHNETSTGVTSRIPLVRKAIDN
                     AKHPALLQVDTISSLGSIDYRHDEWGVDVTVGGSQKGLMLPPGLSFNAISDKAIAASK
                     AAKLARSFWDWSEMLPANANGFFPYTPATNLLYGLREAIHMLEEEGLANVFARHDRHA
                     EATRRAVRAWGLEVLCQVPEEYSSSLTAVLMPPGQDADRFRAVVLEHFDMSLGQGLSK
                     LSGKIFRIGHLGDFNDLSLMGTLAGVEMGLDIAAVPFRKGGAQAAMDYLAQAAAKKQA
                     NRRAA"
     gene            1342..>1784
                     /locus_tag="VF309_08095"
     CDS             1342..>1784
                     /locus_tag="VF309_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7466587.1"
                     /translation="MTLPTPQPVRFARGEDRTRHALGARLAREVDAEVLFDSASRARY
                     STDASIYQVIPVGIVVPRNAEAAIAAMQIAAEEGVAILPRGAGTSQCGQTVGDALVID
                     DSKFVNRVLEVDAATRRAIVEPGKVLDTLNAELRPFGLWYPIDVST"
BASE COUNT          282 a          578 c          620 g          304 t
ORIGIN      
        1 gcgcatcgtc ttgacgcgaa tactctatca ggctcaggcg gcgcggcggt tcgcctgctt
       61 cttcgcggcc gcctgcgcga gatagtccat cgccgcctgc gcgccgccct tgcggaaggg
      121 aacggcggcg atatcgagtc ccatctcgac gccggcgagc gtgcccatca gcgacaggtc
      181 gttgaagtca ccgagatggc cgatgcggaa gatcttgccg gaaagcttcg acaggccctg
      241 gccgagcgac atgtcgaaat gctcgaggac gacggcgcga aagcgatcgg catcctgtcc
      301 cggcggcatc aggaccgccg tgagggacga gctgtattcc tcggggacct ggcaaaggac
      361 ctcgaggccc caggcgcgca cggcgcggcg cgtggcctcg gcatggcgat cgtggcgggc
      421 gaacacgttg gcgaggccct cttcctcgag catgtggatc gcctcgcgca gcccgtacag
      481 gaggttcgtc gcgggcgtgt agggaaagaa gccgttcgcg ttggccggaa gcatctcgct
      541 ccagtcccag aacgagcgcg cgagcttcgc cgccttgctg gcggcgatcg ccttgtcgga
      601 tatcgcgttg aaggaaaggc cgggcggcag catgagccct ttctgcgatc cgcccacggt
      661 gacgtccacg ccccattcgt cgtggcggta gtcgatcgag cccagcgacg agatggtgtc
      721 gacctggagg agcgccggat gcttcgcgtt gtcgatcgcc ttgcgcacca gtgggatccg
      781 gctcgtgacg ccggtggagg tctcgttgtg cacgacgcac acggccttga tggcatgggc
      841 cttgtcggcg gcgagccgcg cctcgatctc gcccgcgtcg acgccgtggc gccagtcgcc
      901 ggggatcatg tcgatttcca gggagagctt cttcgccatc ttctgccaga gcgaggcgaa
      961 gtggccggtc tcgcacatga ggacccggtc gccgggcgag agcgtgttca ccagcgccgc
     1021 ttcccatgcg ccggtccccg acgcgggaaa gatgatcacc ggattgcgcg tctgaaagat
     1081 ccgcttgatg ccttcgagca cttccttgcc gagcttctgg aactcggggc cgcgatggtc
     1141 gatggtggga cggtcgatcg cccgcagcac gcgatcgggc acgttggtgg gaccgggaat
     1201 ctgcaggaag tggcgtccgg cgggcatgga atggctcctc gcgagtaaca agcccgtatg
     1261 caccattgcg gccgggcccg gtttgtcgtc cataatgccg cagcctcgac ccctttggca
     1321 cgaaccccgg taaaagcctt catgaccctt ccgaccccgc aacccgtgcg cttcgcgcgg
     1381 ggcgaggacc gaacccgaca tgctcttggc gcgcgcctcg cgcgcgaggt cgacgccgag
     1441 gtgctcttcg attccgcgag ccgcgcccgc tattcgacgg acgcatcgat ctaccaggtg
     1501 attcccgtgg gaatcgtggt cccgcgcaac gccgaggcgg cgatcgcggc gatgcagatc
     1561 gcggccgagg agggtgtggc gatcctgccg cgcggcgcgg ggacttcgca gtgcgggcag
     1621 acggtcggcg acgcgctcgt gatcgacgac agcaagttcg tgaaccgcgt gctcgaggtc
     1681 gatgccgcca cgcgccgcgc gatcgtcgaa ccggggaagg tgctcgacac gctgaacgcg
     1741 gagcttcgcc cgttcgggct ctggtatccg atcgatgtct ccac
//
LOCUS       DASVII010000622         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_639910,
            whole genome shotgun sequence.
ACCESSION   DASVII010000622 DASVII010000000
VERSION     DASVII010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639910"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VF309_08100"
     CDS             complement(<1..708)
                     /locus_tag="VF309_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7466588.1"
                     /translation="MLVSISHVTRYTLAVPAHYSIQSVRLTPPSFDGQKVMSWAIEMP
                     HIDDAVQFRDCFGNMAHLVSIPEPHSAITITARGLVETQDRAGSVRGLIETAPVRVFK
                     RETPTTAPDDGIRGLAAGIDGKGTIERMHALMTAVTDGVEYLAGVTDSRTSAAEALRE
                     GRGVCQDHAHIFISVARVLGVPARYVNGYFVTGGEEPAQAHHAWAEAWIDGLGWLGFD
                     PANGLCPTERYIRLACGL"
     gene            complement(724..1674)
                     /locus_tag="VF309_08105"
     CDS             complement(724..1674)
                     /locus_tag="VF309_08105"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX7466589.1"
                     /translation="MLGRTANDLYWLARYVERAENMARLIEVGYRIALLPRDGAGYHD
                     EEWRSTLASAGCAQGYFAKHESLNTRDVINYLLFDCNNSSSVKACLSAARRNGRAQRT
                     SLTRDMWESLNSAWIEFGNIKPIGMTASELPRVLDWVRSRSALYRGALLNTILRNDTF
                     FFSQLGTFVERADNTARILDVKYYVLLPELAMVGGGVDNVQWGAILRSVSAHRSYRWV
                     YKENYKPWRIAEYLILNQAMPRSLCSCYHEMAEALDKLASLYGDRFACHATAQATQEI
                     LAKGDIERIFQSGLHEFLEDFISRNNRLGSEIAEAYHFNA"
BASE COUNT          283 a          547 c          605 g          311 t
ORIGIN      
        1 gaggccgcag gcgaggcgga tgtaacgctc ggtggggcaa aggccgttgg cgggatcgaa
       61 cccgagccag cccagtccgt cgatccaggc ctcggcccag gcgtggtgcg cctgggcggg
      121 ctcctcgccc cccgtcacga agtagccgtt aacgtaacgg gcaggcacgc cgaggacgcg
      181 cgccaccgaa atgaagatgt gggcgtggtc ctggcacacg ccgcgcccct cccgcagcgc
      241 ctccgccgcc gaagtgcggg agtcggtgac gccggccagg tattcgaccc cgtcggtcac
      301 cgccgtcatg agcgcatgca tgcgctcgat cgtgcccttc ccgtcgatgc cggcggcgag
      361 cccgcggatg ccgtcgtcgg gcgccgtggt cggcgtctct cgcttgaaca cgcggacggg
      421 cgcggtctcg atgaggccgc gcaccgagcc ggcgcggtcc tgcgtctcga ccagccctcg
      481 cgcggtgatg gtgatggcgc tgtgcggctc cgggatcgag acgagatgcg ccatgttgcc
      541 gaagcagtcg cggaactgca ccgcgtcatc gatgtggggc atctcgatgg cccaactcat
      601 gaccttctgc ccatcgaagg acggcggcgt caggcgcacg ctctggatgg agtagtgggc
      661 gggaaccgcc agcgtgtacc gcgtcacatg ggagatggaa acgagcacgg acgcttcacc
      721 ttctcaggcg ttgaagtgat aggcctcggc gatctcgctg cccaaccggt tgttgcgcga
      781 gatgaaatcc tcgagaaact cgtgcaggcc ggattggaat atgcgctcga tatcgccttt
      841 tgcgagaatc tcttgggtcg cttgcgcggt ggcgtggcag gcgaagcgat cgccgtagag
      901 gctcgcgagt ttgtcgagcg cctccgccat ctcatgatag caggagcaca gcgacctcgg
      961 catcgcctga ttgaggatca gatattcggc gatgcgccag ggcttgtagt tctccttgta
     1021 gacccagcgg tagctgcggt gcgcggagac tgagcgcaat atcgcgcccc actgcacgtt
     1081 gtcgacgccg ccgccgacca tcgcgagctc cggcagcagc acgtaatact tcacgtcgag
     1141 gatgcgggcc gtgttgtcgg cacgctcaac gaaggtgccg agctggctga agaagaaggt
     1201 gtcgttgcgc agaatcgtgt tgagcagcgc gccgcggtag agggccgagc gcgagcgcac
     1261 ccagtcgagg acgcgcggaa gctcgctcgc ggtcattccg atcggcttga tgttgccgaa
     1321 ctcgatccag gcgctgttga ggctctccca catgtcgcgg gtgagcgacg tgcgctgagc
     1381 gcgaccgttg cggcgcgcgg ccgacaggca cgccttgacg ctggaggagt tgttgcagtc
     1441 gaacaggagg tagttgatga cgtcgcgcgt gttgagcgac tcgtgcttgg caaagtagcc
     1501 ctgcgcgcag ccggcgctcg cgagcgtcga gcgccattcc tcgtcgtggt acccggcgcc
     1561 gtcgcgcggc aaaagcgcga tgcggtaccc gacctcgatc aggcgcgcca tgttctcggc
     1621 gcgctcgacg tatcgcgcga gccagtagag gtcgttggcc gtccgtccca acatgcgagt
     1681 gtctggcttt cgccgttcct tagcgttctt ccctagcgtt cagtcctgca ggacccaggt
     1741 gtcttt
//
LOCUS       DASVII010000623         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_712311,
            whole genome shotgun sequence.
ACCESSION   DASVII010000623 DASVII010000000
VERSION     DASVII010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712311"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..>1656
                     /gene="mnmC"
                     /locus_tag="VF309_08110"
     CDS             54..>1656
                     /gene="mnmC"
                     /locus_tag="VF309_08110"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEX7466590.1"
                     /translation="MVTVPTARLERSADGTPFSPEFQDVYHSSHGGLQQARHVFLRGN
                     DLPARWQGRDSFVIVETGFGLGVNFLAVWHAWRADPSRSRRLHFVSVESHPFTREDLA
                     AALGPFAEIEELASALVIAWPPPLAGFHRAHFDGGNVILTLLFGEARAMLPQLVAQGD
                     AFFLDGFSPARNPGIWSSEVVREIARLAAPGATLATWTVAGGVRTALADAGFSIEKRP
                     GFAAKREMLVGRRALRGEKHEREGGGARPGESSGRRAVVIGAGLAGTLTAERLASRGW
                     SVELVDAREERSVAAVGLVRPIANLRDAVNAQVSRSAFLYALQHFRALQHGGHHLAWN
                     RCGVLQLAGDDDEASRFAAIAQSQGYPSAFLAHVDRDAARAIARCDVRGPGWWFPGGA
                     WVAPASLAVASLARAGDKVQRRIGRHVARLEREGSAWRALDANDRVIAEAPVMVVASA
                     VDAKRLVPDARLALSSVRGQVTYLPPSPRRTLDVIVSGSGYVAPLPGGGHCVGASYHH
                     DDDDASVRAADHRGNLDRAESMLPGF"
BASE COUNT          201 a          600 c          609 g          246 t
ORIGIN      
        1 gccgttgacg ccggcggcgc ggccctcggc ccgcgccgcc gtatgatgcg cgcatggtca
       61 cggtccccac ggcgcgcctc gaacgatcgg ccgacggcac tcccttctcg ccggaattcc
      121 aggacgtcta ccactcgtcc cacggcggcc tgcagcaggc gcgccatgtc ttccttcgcg
      181 gcaacgacct gccggcgcgc tggcaggggc gcgattcgtt cgtcatcgtc gagacgggct
      241 tcggcctcgg agtgaatttc ctcgccgtgt ggcacgcgtg gcgcgcggac ccgtcgcgat
      301 cgcggcgcct gcacttcgtc tccgtcgaga gccatccctt cacgcgcgag gacctggccg
      361 cggcgctcgg gcccttcgcg gaaatcgaag agctcgcgag cgcgctcgtc atcgcctggc
      421 cgccgccgct cgccggattc catcgcgcgc atttcgacgg cggcaacgtg atcctcaccc
      481 tcctcttcgg cgaggcgcgc gcgatgctgc cgcagctcgt cgcccagggc gacgcgttct
      541 tcctcgacgg gttctcgccc gcgaggaatc ccgggatctg gtcgtcggag gtggtgcgcg
      601 agatcgcgcg cctcgcggcg ccgggcgcga cgctcgcgac atggaccgtg gcgggcggcg
      661 tgcgcacggc gctcgccgac gccggatttt ccatcgagaa gcgccccggt ttcgccgcga
      721 agcgcgagat gctggtggga cggcgcgcgc tgcgcggcga gaaacacgag agggaaggag
      781 gcggcgcgag gcccggggaa agttccgggc ggcgcgccgt cgtgatcggc gcggggctcg
      841 cgggaacgct caccgccgag cggctcgcat cgcgcggatg gagcgtcgag ctggtcgatg
      901 cgcgcgaaga gcgcagcgtc gccgccgtgg ggctggtgcg ccccatcgcc aacctgcgcg
      961 acgcggtgaa cgcgcaggtc tcccgaagcg cctttctcta cgcgctccag cacttccgcg
     1021 cgctccagca tggcggccat cacctcgcgt ggaaccgctg cggcgtgctg cagcttgccg
     1081 gcgacgacga tgaggcctcc cgcttcgcgg cgatcgcgca atcccagggc tatccctcgg
     1141 cgtttctcgc gcatgtcgat cgcgatgcgg cgcgcgcgat cgcccgctgc gacgtgcgcg
     1201 ggccaggctg gtggtttccc ggcggggcgt gggtggcgcc ggcaagcctc gccgtcgcga
     1261 gcctcgcgcg cgccggcgac aaggtgcagc ggcgcatcgg gcggcacgtc gcgcgtctcg
     1321 agcgcgaggg ctcggcgtgg cgcgcgctcg acgccaacga tcgcgtgatc gccgaggctc
     1381 cggtgatggt ggtcgcgagc gctgtcgacg cgaagcggct ggtccccgac gcgcgcctcg
     1441 cgctgagcag cgtgcgcggc caggtgacgt acctgccgcc gtcgccgcgc cgcacgctcg
     1501 atgtgatcgt gagcggctcg ggctatgtgg cgccgcttcc cggaggcggc cattgcgtgg
     1561 gcgcgagcta tcaccacgac gatgacgacg cctcggtgcg cgccgccgac catcgcggaa
     1621 acctcgatcg cgccgagtcg atgctgcccg ggttca
//
LOCUS       DASVII010000624         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_712325,
            whole genome shotgun sequence.
ACCESSION   DASVII010000624 DASVII010000000
VERSION     DASVII010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712325"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1834)
                     /gene="glyS"
                     /locus_tag="VF309_08115"
     CDS             complement(<1..1834)
                     /gene="glyS"
                     /locus_tag="VF309_08115"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX7466591.1"
                     /translation="MDATLLVELFCEELPPKALKRLGEAFAEGLHRGLAKREMLGADS
                     AARAFATPRRLAASIARVKGASAPRAVQVKLMPAAVGLDASGRPTPALLKKLDAAGLA
                     GTDVSKLQRRLDGKAEMLFADATTPAMPLAQALQGALEEAIAGLPIPKVMSYQLADGE
                     TTVQFVRPAHGLVALHGGEVVGVTALGLASGRTTHGHRFQGARNIELADAEAYEASLE
                     REGRVIASFAARRGEIARQLEEKGRALSPGFVPPEALLDEVTGLVEWPAVYIGSFEEE
                     FLAVPQECLVLTMQQNQKYFPLAGPDGKLLAKFLVVSNLRLDDPADIVQGNERVVRPR
                     LADARFFFETDKKVKLADRVPQLASRTYHNKLGTDLERVERLRKLASRVQSMLPRGEK
                     GRPYADRAALLAKADLVTLMVGEFPELQGIMGKYYAEADGEEPSVVRAIEQHYWPRFS
                     GDQLPVGDVSIAVALADKLEAIAGMFGIGQQPTGDKDPFALRRHAIGIIRILVEGGFD
                     LALDVLVNHAFEVFPRGMIGDAHTDVRAFIHERLRGYLREQGYSANEVESVLSMNPVR
                     LSPIPRQLAAVRDFMKLPEAESLVAANKRVANILRQASGKGESFA"
     gene            complement(1834..>2673)
                     /gene="glyQ"
                     /locus_tag="VF309_08120"
     CDS             complement(1834..>2673)
                     /gene="glyQ"
                     /locus_tag="VF309_08120"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX7466592.1"
                     /translation="QPYDMEVGAGTSHTATFLRALGPEPWRAAYVQPSRRPKDGRYGD
                     NPNRMQHYYQFQVVLKPSPPEILDLYLGSLEAIGFDLAKNDVRFVEDDWENPTLGAWG
                     LGWEVWLNGMEVTQFTYFQQVGGLDCKPITGEITYGLERLAMYLQGVENVFDLVWTEW
                     KDPSGRMHRLTYRDVFHQNEVEQSAYNFEESDPAALFRHFGDHESTAKRLMDAHLALP
                     AYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNLAKAVAQSYYESRERLGFPMLPR
                     GSA"
BASE COUNT          417 a          915 c          901 g          440 t
ORIGIN      
        1 tggcaaacga ttcacctttg cctgaagcct gccgaagtat gttggccacc cgcttgttgg
       61 cagcgaccag gctttccgct tcgggcaact tcatgaaatc gcgaaccgcc gcgagctgcc
      121 tcggaatcgg gctgagccgc accggattca tcgacagcac cgattccacc tcgttggcgg
      181 aatatccctg ctcgcgcaga tagcccctga gccgctcgtg gatgaaggcc cgcacgtccg
      241 tgtgcgcgtc gccgatcatt ccccgcggaa agacctcgaa cgcatggttc accaggacgt
      301 cgagcgcgag gtcgaacccg ccttcgacga ggatgcggat gatcccgatc gcatggcggc
      361 gcagcgcgaa cggatccttg tcgcccgtgg gttgctggcc gattccgaac atccccgcga
      421 tcgcttccag cttgtcggcc agggcgaccg cgatgctcac gtcgccgacg ggaagctggt
      481 ctccggaaaa acgcggccag tagtgctgct cgatcgctcg cacgacggaa ggctcctcgc
      541 catcggcctc ggcgtagtac ttccccatga tcccctggag ctccggaaac tcgccgacca
      601 tcagcgtgac gaggtcggcc ttcgcgagga gcgcggcgcg atccgcgtag gggcgcccct
      661 tctcgccgcg aggcagcatc gattgaacgc ggctcgcgag cttgcgcagg cgctcgacgc
      721 gctccaggtc cgtgccgagc ttgttgtggt acgtcctcga tgccagctgc ggcacgcggt
      781 cggcgagctt caccttcttg tcggtctcga agaagaagcg cgcgtcggcg agccgcgggc
      841 gcaccacgcg ctcgttgccc tgcacgatgt ccgcggggtc gtcgagtcgc aggttcgaca
      901 cgacgagaaa tttcgcgagg agcttcccgt cgggccccgc cagcggaaag tacttctggt
      961 tctgctgcat ggtgaggacg aggcattcct gcggcaccgc gaggaattcc tcctcgaagg
     1021 atccgatgta gaccgcgggc cactccacca ggcccgtgac ctcgtcgagc agggcttccg
     1081 gcggcacgaa gcccggcgag agcgcgcggc ccttctcctc gagctgccgc gcgatctcgc
     1141 cgcgacgcgc ggcgaacgac gcgatgacgc ggccctcacg ctccagggac gcctcgtagg
     1201 cctcggcgtc ggcgagttcg atatttcgcg cgccctggaa gcggtgcccg tgagtcgtgc
     1261 gccccgatgc gaggccgagt gccgtcacgc cgaccacttc gccgccgtgg agcgcgacca
     1321 ggccgtgggc gggccgcacg aactgcacgg tggtctcgcc gtcggcgagc tggtagctca
     1381 tcaccttcgg aatcggcagg cccgcgatcg cctcttcgag cgcaccctgc agggcctggg
     1441 cgagcggcat cgcgggcgtg gtcgcgtcgg cgaagagcat ctcggccttg ccgtcgaggc
     1501 gccgctgcag cttcgagaca tcggtgcccg cgaggcccgc cgcatcgagc ttcttcagca
     1561 gcgcgggcgt gggacggccc gaagcgtcga gcccgaccgc ggccggcatg agcttcacct
     1621 gcacggcgcg cggcgccgac gcgcccttca cgcgagcgat cgaggccgcg aggcggcgcg
     1681 gcgtcgcgaa agcgcgcgcc gcgctatccg cgccgagcat ctcgcgcttc gcaaggcccc
     1741 ggtgcaggcc ttcggcgaag gcctcgccga ggcgcttcag cgccttcggc ggaagctctt
     1801 cgcagaagag ctcgacgagg agcgttgcgt ccatcacgcg ctcccgcggg gcagcatcgg
     1861 aaatccgagg cgctcgcgcg attcgtagta gctctgcgcc accgccttgg ccaggttgcg
     1921 aatgcgcccg atgtacgcgg cgcgctccgt cacggagatg gcgccgcgcg catcgagcag
     1981 gttgaacgtg tgcgcggcct tcagcacctg ctcgtaggcg ggaagcgcga gatgggcgtc
     2041 catcagccgt ttcgcggtcg actcgtgatc gccgaaatga cggaacaggg cggcggggtc
     2101 ggattcctcg aagttgtacg ccgactgctc gacctcgttc tggtggaaga cgtcgcgata
     2161 cgtgaggcga tgcatccttc ccgacgggtc cttccactcg gtccacacga ggtcgaagac
     2221 gttctccacg ccctgcaggt acatggcgag gcgctcgagc ccgtaggtga tctcgccggt
     2281 gatcggcttg cagtcgagcc cgcccacctg ctggaagtag gtgaactgcg tgacttccat
     2341 cccgttcagc cacacctccc acccgagccc ccacgcgccg agcgtcggat tctcccagtc
     2401 gtcctcgacg aagcgaacgt cgttcttcgc gaggtcgaag ccgatcgcct cgagcgagcc
     2461 caggtagaga tcgaggatct ccggcggcga gggcttgagc accacctgga attggtagta
     2521 gtgctgcatg cggttgggat tgtcgccgta gcgcccgtcc ttgggacggc gcgagggctg
     2581 cacatacgcg gcgcgccacg gctccggccc cagggcgcgc aggaacgtgg cggtgtgcga
     2641 agtgcccgcg cccacttcca tatcgtaggg ctg
//
LOCUS       DASVII010000625         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_245268,
            whole genome shotgun sequence.
ACCESSION   DASVII010000625 DASVII010000000
VERSION     DASVII010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245268"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF309_08125"
     CDS             complement(<1..402)
                     /locus_tag="VF309_08125"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466593.1"
                     /translation="MPDAVTAERPNPSLALLLLPKFLTARARTMANERGRTARFTLLG
                     VVGLGFWAFIFTVLYKLLVYFRSVPDLGPFMAGKLLGLILIGFFSLLMLSSIITALSS
                     FFLARDLDLLVAAPVDWLNLYGAKLLETGVNA"
     gene            complement(395..1153)
                     /locus_tag="VF309_08130"
     CDS             complement(395..1153)
                     /locus_tag="VF309_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466594.1"
                     /translation="MISLRKLTKRYGSFTAVDAIDLEVPRGELFGFLGPNGAGKTTTL
                     RMIAGILRPTAGEISIAGVDLVADPIGAKMQLGFIPDRPFIYEKLTGTEFLRFVAGLY
                     DQEGPEVEHRARELMALFDLEEWRDELVESYSHGMRQKLIISSAFVHRPEVIVVDEPM
                     VGLDPKSAKILKDLFREYTRRGHTIMMSTHTLEVAETLCDRVAIIAKGTIRACGTMAE
                     LRSRADADTQGLEEIFLRLTGENAARELVEVLDA"
     gene            complement(1181..>1969)
                     /locus_tag="VF309_08135"
     CDS             complement(1181..>1969)
                     /locus_tag="VF309_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX7466595.1"
                     /translation="LLGMTMERRPYEVAFENWKAEKLLRGTEVAAGDDIYETSAIAAN
                     KTLKTNLRELIVRLSGVELSEFNVKGYRQRLRGDTLTITREDSAALRALYLLPYGARA
                     TVASVFLGPEPLLETTSPEIVALASRLRGNTADPAVVAERINRWVYDSLRKVITVGVP
                     SALGTLRARRGDCNEHAQLYVALARAAGIPARVAAGLAYLDGKFYYHAWPEVWLERWV
                     AVDPTFGQFPADAAHLRFTVGGISHQVELLRLMGSLHIDVLSAH"
BASE COUNT          365 a          666 c          629 g          309 t
ORIGIN      
        1 cgcgttcacc cccgtctcga gcagcttcgc gccgtagagg ttcagccaat cgacgggcgc
       61 cgcgacgagc aggtcgagat cccgcgcgag gaaaaagctc gagagcgccg tgatgatgct
      121 cgagagcatg agcagcgaga agaagccgat gagtatcaac ccgagcagct tgcccgccat
      181 gaatgggccg aggtcgggca ccgagcggaa atagacgagc agcttgtaca ggacggtgaa
      241 gatgaacgcc cagaagccca ggccgactac cccgagcagc gtgaagcgcg ccgtgcgtcc
      301 gcgctcgttc gccatggtgc gcgcacgagc ggtgagaaac ttcggcagca gaaggagcgc
      361 gagcgacggg ttgggacgct ccgccgtcac cgcgtcaggc atcgaggacc tcgaccagct
      421 cgcgcgcggc gttctcgccg gtcagccgga ggaagatctc ctccagcccc tgcgtgtccg
      481 cgtcggcccg cgagcgcagc tccgccatcg tgccgcaggc gcggatggtc cccttcgcga
      541 tgatcgcgac gcgatcgcac agcgtctcgg ccacctcgag ggtgtgcgtg ctcatcatga
      601 tggtgtgccc gcgccgcgtg tactcgcgga acagatcctt gagaatcttc gccgacttcg
      661 gatcgagccc caccatcggc tcgtccacca cgatcacctc ggggcggtgc acgaacgcgc
      721 tggagatgat cagcttctgg cgcatgccgt ggctgtagct ttccacgagc tcgtcgcgcc
      781 actcctccag gtcgaacagc gccatcaact cgcgcgcgcg atgctcgacc tccggcccct
      841 cttgatcata gagccccgcg acgaagcgga gaaactccgt gccggtcagc ttctcgtaga
      901 tgaacggccg gtcggggatg aagccgagct gcatcttcgc gccgatcgga tcggccacga
      961 ggtccacacc ggcaatcgag atctcgccgg cggtggggcg gagaatcccg gcgatcatcc
     1021 gaagcgtcgt cgtcttgccc gcgccgttcg ggccgaggaa accaaacagc tcaccgcgcg
     1081 gaacctcgag gtcgatggcg tcgactgcgg tgaagctgcc gtaacgcttc gtaagcttac
     1141 ggaggctgat catgcaagga ggagagcggc gcgcgagcgc ctagtgagcg gagagaacgt
     1201 cgatgtgcag tgagcccatc agtcgcagca gctcgacctg atgactaatt ccaccgacgg
     1261 tgaatcgcag atgtgcggcg tcggcgggaa actgcccgaa cgtcggatcg accgcgaccc
     1321 atcgctctaa ccagacttcg ggccaggcat ggtagtaaaa ctttccatcc aagtaggcca
     1381 ggccggcggc caccctcgcg ggaatccccg cggcccgcgc cagcgccaca tacagctgag
     1441 catgctcgtt acagtcgcct cggcgggcgc gcagagtgcc aagcgcactc ggcacgccaa
     1501 cggtgatcac tttccgcagc gagtcgtaca cccatctgtt gatgcgctcg gccactaccg
     1561 ccggatcggc agtgttcccg cgcagccgcg acgcgagcgc gacgatctcc gggctcgtcg
     1621 tctccagcag tggctcgggg ccgaggaaca ccgacgcgac cgtggcgcgc gcgccgtagg
     1681 gcagcaggta gagcgccctg agggccgccg aatcctcgcg cgtgatcgtc aacgtgtcgc
     1741 cgcgcagacg ctgccggtac cccttcacat tgaactcact caactccacg ccggacaggc
     1801 gcacgatcag ctcacggagg ttcgtcttga gcgtcttgtt cgcggcgatc gccgacgtct
     1861 cgtagatgtc gtcaccagcc gcgacttcgg tgccgcgcag cagcttctcc gccttccagt
     1921 tctcgaatgc cacctcatag ggccggcgct ccatcgtcat gcccagcag
//
LOCUS       DASVII010000626         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_568771,
            whole genome shotgun sequence.
ACCESSION   DASVII010000626 DASVII010000000
VERSION     DASVII010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568771"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF309_08140"
     CDS             <1..425
                     /locus_tag="VF309_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX7466596.1"
                     /translation="EAGVNGGPPGDLYVVMHLKPHGVFQRDGDDLHCEMPISFTQAAL
                     GGEIEIPTLDGSAKIKVPPETQSGQVFRLRGKGIKGVRSSYAGDLMCEVVVETPVRLT
                     DRQKDLLRELEEINRKDANRHNPRAKGFMDKVREFFAG"
     gene            515..>1546
                     /locus_tag="VF309_08145"
     CDS             515..>1546
                     /locus_tag="VF309_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466597.1"
                     /translation="MLGRTFMAALALAAANLAAAQGVSDSQVVLGQSVALTGPAAQLG
                     LDMQLGANLYFNQVNAHGGVNGRRIVLKTLDDGYEATRAADNTRKLINDEKVFALFGY
                     VGTPTSQASLPIFTEARVPFVGPFTGAELLRNPVNPYVFNVRASYFDETEAIVQHLTA
                     MSVNRIAVFYQNDAYGLAGLAGVERALKKRNLEVIAKGTVERNTVDVKKAIDDIRKAN
                     PQAVVMIGAYKGCAAFIRDMKRSGANPTFWNVSFVGSKALAKELDKDGRGVQISQVVP
                     FPWDRSIPVVKEYNRLIVLDGKSEPNFSSLEGFISAKVMVEGLRRAGKNLTREGLVHA
                     LESMDSYDVG"
BASE COUNT          297 a          513 c          507 g          229 t
ORIGIN      
        1 gcgaagccgg cgtgaacggc ggccctcccg gggacctcta cgtcgtcatg cacctgaagc
       61 ctcacggcgt cttccagcgc gatggcgacg acctccactg cgagatgccc atcagcttca
      121 cgcaggcggc gctcggcggc gagatcgaga tccccacgct cgacggctcg gccaagatca
      181 aggtgccgcc ggagacgcag tcggggcagg tgttccgcct gcgcggcaag ggcatcaagg
      241 gcgtgcgctc cagctatgcc ggcgacctca tgtgcgaggt ggtggtggag acgccggtgc
      301 gcctcaccga tcgccagaag gacctcctgc gcgagctgga ggagatcaac cgcaaggacg
      361 ccaaccggca caacccgcgc gccaagggct tcatggacaa ggtgcgcgaa ttcttcgccg
      421 gatgacagac gcaacacgcc ggccagcgtg aaattccctc ccgcggcgtt atgatgcgcg
      481 catcataccc gcagctaaac gggagggaga aaccatgctt ggccgcacat tcatggcagc
      541 gctcgcgctc gccgccgcca acctcgccgc ggcccaaggg gtcagcgatt cgcaggtagt
      601 cctcggccag tcggtcgccc tcaccggtcc cgcggcgcag ctcgggctcg acatgcagct
      661 cggggccaat ctctacttca accaggtcaa tgcgcacggc ggcgtgaacg gccgcaggat
      721 cgtgctgaag accctcgacg acggctacga ggcgacgcgc gccgccgaca acaccaggaa
      781 gctcatcaac gacgagaagg tgttcgcgct gttcggctac gtgggtacgc cgacctcgca
      841 ggcctcgctg ccgatcttca ccgaggcgcg cgtgcccttc gtgggcccct tcaccggcgc
      901 ggagctcctc aggaacccgg tcaatccgta cgtgttcaac gtgcgcgcca gctacttcga
      961 cgagaccgag gcgatcgtgc agcacctgac cgccatgagc gtgaaccgga tcgcggtctt
     1021 ctaccagaac gacgcgtacg gcctggcggg cctggccggc gtcgagcgcg cgctgaagaa
     1081 gcgcaacctc gaggtgatcg ccaagggcac ggtggaacgc aacacggtcg acgtgaagaa
     1141 ggcgatcgac gacatcagga aggcgaatcc gcaggcggtg gtgatgatcg gcgcctacaa
     1201 gggctgcgcc gcgttcatcc gcgacatgaa gcgctccggc gcgaacccga ccttctggaa
     1261 cgtctccttc gtcggcagca aggcgctcgc caaggagctc gacaaggacg gccgcggcgt
     1321 gcagatctcg caggtggtgc cgttcccgtg ggatcgatcg atccccgtgg tgaaggaata
     1381 caaccggctc atcgtccttg acgggaaatc ggaacccaac ttctcgagcc tcgagggatt
     1441 catttccgcc aaggtgatgg tcgaagggct gcggcgcgcg ggcaaaaacc tcacgcgcga
     1501 gggcctggtg catgcccttg aaagcatgga ttcgtacgac gtcggc
//
LOCUS       DASVII010000627         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_568814,
            whole genome shotgun sequence.
ACCESSION   DASVII010000627 DASVII010000000
VERSION     DASVII010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568814"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VF309_08150"
     CDS             <1..1074
                     /locus_tag="VF309_08150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7466598.1"
                     /translation="FSSSHALEVETKDGRKVVSFDHCIIAAGSQSAQIPGFPYDDPRL
                     MDSTGALELKDVPKRLLIIGGGIIGLEMATVYDALGSKVTVVEFMDRLIPGADPEIVK
                     PLAKRIEKRYQKILLKTKVAKIESTSVGLKAYFEAAGGGEAPAPEIYDRILLAVGRRP
                     NGKSIAAEKAGVTVNERGWIPVNNQMRTNVPHIFAIGDIVGEPMLAHKATHEAKLAAE
                     VIAGMSHHAWEARTIPSVAYTDPEVAWMGLSEPEAKEQGVEYDKAVFPWAASGRARGM
                     GREEGLTKVLYEKGTKRILGAGIVGVNAGELIAETVLALEMGADAEDIGLTIHPHPTL
                     SETVFFTAEMAEGSITDMMPPKR"
     gene            1074..1739
                     /locus_tag="VF309_08155"
     CDS             1074..1739
                     /locus_tag="VF309_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7466599.1"
                     /translation="MPERSTWDAEARAAFKEARDPKALGTAVIRFHARVDEVVDATLR
                     GHSVKVACSRGCSYCCSLQVQVAPHEAFALAQWLTKNFSAGKLAAVTGKLRDNAAKTR
                     AMGDAARKRSNLACALLGDDGACTAYEARPAQCRRFNSTDLEICKASYANPADDSIES
                     PNHPAVAHNAAVIVTQANQAVRDAGLDAAPVDMNLALLEALENPKAWRRWRDGKKPFT
                     GPR"
     gene            complement(1723..>2486)
                     /locus_tag="VF309_08160"
     CDS             complement(1723..>2486)
                     /locus_tag="VF309_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466600.1"
                     /translation="AFWLTVRFTADRFVSSAQEVTTAPSARRRGARPARRFRGGLARI
                     VIAKELVLIARDPMLIAKSLLQALYLLPMMVVLIRNADAAPVLAATLVVMVSNLAGTF
                     AWLTISGEEAPELLESSPVSREHVRWLKVGAAVVPVGAVLLPFLAWYAWLSLEMFAIV
                     AVAAAAAITSTAVIQVWTGKPGSPRDLRARQKRGVAMSFVEFFGSLGWAAACFLAISG
                     YYRYIPAGVALGVAGPGAAWLVRQWRDRNEFSAGP"
BASE COUNT          453 a          867 c          843 g          323 t
ORIGIN      
        1 ttctcgtcgt cgcatgcgct cgaggtcgag acgaaggacg gcaggaaggt cgtatcgttc
       61 gaccactgca tcatcgcggc ggggtcgcag tcggcgcaga ttcccggatt tccctatgac
      121 gatccgcgcc tcatggattc cacgggcgcc ctcgagctga aggacgtccc gaagcgcctt
      181 ctcatcatcg ggggcggcat catcggcctc gagatggcga cggtgtacga cgcgttggga
      241 tcgaaggtga ccgtggtgga gttcatggac cggctcatcc ccggcgccga tcccgagatc
      301 gtgaagcccc tcgccaagcg catcgagaag cgctaccaga agattctcct gaagacgaag
      361 gtcgcgaaga tcgagtcgac ctccgtgggc ctgaaggcgt acttcgaggc ggccggcggc
      421 ggcgaagcgc ccgcgccgga gatctacgat cgcatcctgc ttgcggtggg ccggcgcccc
      481 aacggcaaga gcatcgccgc ggagaaagcg ggagtcaccg tgaacgagcg cggctggatc
      541 cccgtgaaca accagatgcg caccaatgtg ccgcacatct tcgcgatcgg cgacatcgtg
      601 ggcgagccca tgctcgcgca caaggcgacc cacgaggcga agctcgccgc ggaagtgatc
      661 gccggcatga gccaccacgc gtgggaggcg cgcaccatcc cgagtgtcgc ctacaccgat
      721 cccgaggtcg cgtggatggg attgtccgag cccgaggcga aggagcaagg cgtcgagtac
      781 gacaaggccg tgttcccctg ggccgcgagc gggcgcgcgc gcggcatggg gcgcgaggaa
      841 ggcctcacca aggtgctcta cgagaagggc acgaagcgca tcctcggcgc gggcatcgtc
      901 ggcgtgaacg cgggcgagct catcgccgag accgtgctcg ccctcgagat gggcgcggac
      961 gccgaggaca tcggcctcac catccatccg catcccacgc tctcggagac ggtcttcttc
     1021 acggcggaga tggccgaagg ctcgatcacc gacatgatgc cgccgaagcg ctgatgcccg
     1081 agcggtccac gtgggacgcc gaggcgcgcg cggccttcaa ggaggcccgc gatccgaagg
     1141 ccctcgggac ggcggtcatc cgcttccacg cgcgcgtcga cgaggtcgtc gacgcgacgc
     1201 tgcgcggcca ttcggtgaag gtcgcctgca gccgcggctg cagctactgc tgcagcctgc
     1261 aggtgcaggt cgcaccccat gaagccttcg ccctcgccca gtggctcacg aagaatttca
     1321 gcgccgggaa actcgccgcc gtgacgggaa agctgcgcga caacgcggcg aagacgcgcg
     1381 cgatgggtga cgcggcgcgc aagcgatcca acctcgcgtg cgccctcctc ggcgacgacg
     1441 gcgcctgcac cgcctacgag gcgcgccccg cgcaatgccg ccgcttcaac tcgacggacc
     1501 tcgagatctg caaggcctcc tatgcgaatc ccgccgacga ttccatcgag agcccgaacc
     1561 atcccgcggt cgcgcacaat gccgccgtga tcgtcaccca ggccaaccag gccgtgcgcg
     1621 atgcggggct cgacgcggcg cccgtcgaca tgaacctcgc gctcctcgaa gcgctcgaga
     1681 atcccaaggc gtggcggcgc tggcgcgacg gcaagaaacc gttcacgggc ccgcgctgaa
     1741 ttcgttgcgg tcgcgccact ggcgcacgag ccaggccgcc cccgggcccg cgacccccag
     1801 ggccacgccc gccgggatgt atcggtagta gcccgagatc gccaggaagc acgccgcggc
     1861 ccaacccagg ctgccgaaga actcgacgaa gctcatcgcg acgccccgct tctggcgcgc
     1921 gcgcaggtcg cgcggcgatc ccggtttgcc ggtccacacc tggatcacgg cagtcgaggt
     1981 gatggccgcc gctgcggcca cggcgacgat ggcgaacatt tccagggaca gccacgcgta
     2041 ccacgcgagg aacggaagga gcaccgcgcc cacgggcacg acggcggcgc ccaccttgag
     2101 ccaacgcacg tgctccctcg agacgggcga cgattccagc agctccggcg cctcctcgcc
     2161 gctgatcgtg agccacgcga aagtccccgc gaggttcgag accatcacca cgagcgtcgc
     2221 ggcaagcacc ggagccgcat cggcattgcg gatcaggacc accatcatcg gcaggagata
     2281 gagggcctga agcagcgact tcgcgatcag catcgggtcg cgcgcgatga ggaccagctc
     2341 cttggcgatg acgatccggg caagaccccc gcgaaatcgg cgagcggggc gcgccccgcg
     2401 ccgccgcgcc gatggcgccg tcgtgacttc ctgggcggag ctcacgaatc gatcggcggt
     2461 gaagcgcacg gtgagccaga aggcgc
//
LOCUS       DASVII010000628         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_100482,
            whole genome shotgun sequence.
ACCESSION   DASVII010000628 DASVII010000000
VERSION     DASVII010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100482"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="atpA"
                     /locus_tag="VF309_08165"
     CDS             complement(<1..977)
                     /gene="atpA"
                     /locus_tag="VF309_08165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7466601.1"
                     /translation="MQINPSEISELIKSKIQNLQVAAEKRTEGTVISVTDGICRVHGL
                     ADVMQGEMLEFPKNTFGLALNLERDSVGAVVLGAYEHISEGDTVKATGRILEVPVGPE
                     LIGRVVNSLGIPIDGKGPINAKMTEPIEKVAPGVIARKSVSQPVQTGLKSIDAMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGKDLICVYVAVGQKASTIANVVRKLEEFGAM
                     AYTIVVAASASDPAAMQYIAPYAGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNPDYVEKFTKGAVKGKTGSLTA"
     gene            complement(994..>1535)
                     /locus_tag="VF309_08170"
     CDS             complement(994..>1535)
                     /locus_tag="VF309_08170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX7466602.1"
                     /translation="PMAEIATVARPYAEALFRAAIEKNALAAVSDSVALLAAAAHDGQ
                     MHSILLNPRVSATQKKEIFDAAAGGRLDDLTKRLLAVLVDGHREVLIGSIAEQFDELK
                     NEHEKILRARITSAQPLSDPQRDEIVAALERRYGKKVEADLDVDPELLGGARVQVGDQ
                     VIHASVRDALAQMAAALTR"
BASE COUNT          219 a          505 c          524 g          287 t
ORIGIN      
        1 gcggtcagcg agccagtctt gcccttcacc gcgcccttgg tgaacttttc gacgtagtcc
       61 ggattcacgc gcgcggcgcg ctcgagcagc ctcgagtgca ggtagaacac gtcgcccgga
      121 tacgcctcgc ggcccggcgg acggcgcagc agcagcgaca cctggcggta cgcccaggcc
      181 tgcttggtca ggtcgtcgta gatgatgagg gcgtcctggc cgcgatcgcg gaagtactcg
      241 cccatggtgc agcccgcata gggcgcgatg tactgcatcg cggcgggatc ggaagccgac
      301 gcggcgacga cgatcgtgta ggccatcgcg ccgaactcct cgagcttgcg cacgacgttc
      361 gcgatggtcg aggccttctg cccgacggcg acgtagacgc agatgaggtc cttgcccttc
      421 tggttgatga tcgcgtccac ggccaccgcg gtcttgcccg tctggcgatc gccgatgatg
      481 agctcgcgtt gcccgcggcc gaccggcacc atcgcgtcga tggacttcag tcccgtctgc
      541 accggctgcg aaacggactt ccgcgcgatc acgcccgggg cgaccttctc gatcggctcg
      601 gtcatcttgg cgttgatcgg gcccttgccg tcgatcggga tgccaaggga attcaccacg
      661 cggccgatga gctccgggcc cacgggcact tcgagaatgc gcccggtcgc cttgacggtg
      721 tcgccctcgg agatgtgctc gtaggcgccc agcaccaccg caccgaccga atcgcgctcc
      781 aggttgaggg cgaggccgaa ggtgttcttg ggaaattcca gcatctcgcc ctgcatcacg
      841 tcggcaaggc cgtgaacgcg gcagatgccg tcggtcacgg agatcacggt cccctcggtg
      901 cgcttttcgg cggcgacctg caggttctgg atcttcgact tgatgagctc gctgatttcg
      961 gaaggattga tctgcatgtg ttgctcctag tgcctagcgc gtgagggccg cggccatctg
     1021 ggccagggcg tcccgcacgg aggcgtggat gacttgatct ccgacctgca cgcgcgcccc
     1081 gcccagcagc tcgggatcga cgtcgaggtc ggcttcgact ttcttgccgt agcggcgctc
     1141 gagcgcggcg acgatctcgt cgcgctgcgg gtcgctcagc ggctgcgcgc tggtgatgcg
     1201 cgcgcgcagg atcttttcgt gctcgttctt cagctcgtcg aactgctcgg cgatggagcc
     1261 gatcagcacc tcgcggtgac cgtcgacgag gaccgcgagc aagcgcttcg tgaggtcgtc
     1321 gagcctgccg cctgcggcgg catcgaagat ctccttcttc tgggtggcgc tcaccctggg
     1381 gttgagcagg atcgagtgca tctgcccgtc gtgcgcggcc gccgcgagga gcgcgacgct
     1441 gtcgctcacc gcggcgagcg cgttcttctc gatcgccgcg cggaagaggg cttccgcata
     1501 gggccgcgcg acggttgcga tttcggccat gggct
//
LOCUS       DASVII010000629         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_208659,
            whole genome shotgun sequence.
ACCESSION   DASVII010000629 DASVII010000000
VERSION     DASVII010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208659"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VF309_08175"
     CDS             <1..466
                     /locus_tag="VF309_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7466603.1"
                     /translation="GIILLAQFAVGSHGARSMKDDPAMSSAAVARRLAPAAQVVIDPN
                     APAPAAAAPASTPALASVTIPPPAAAATPSAGSGKATYSSVCSACHGTGVAGAPKLGD
                     KAAWAPRIKTGIEALHASALKGKGAMPPKGGNAAIADADVKAAVDYMVAAAK"
     gene            483..1289
                     /locus_tag="VF309_08180"
     CDS             483..1289
                     /locus_tag="VF309_08180"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX7466604.1"
                     /translation="MGDLQGCYEPLARLVDSLKFDPSRDRLWFVGDLVNRGPDSLACL
                     RYVKSLGDSAVTVIGNHDFHLLCVAEGIEKRRKRDTIDAVLEAPDRAEMLEWLRHRPL
                     MHVEGGFALVHAGLLPQWSVPRARALAAEVESRLRAPGYRALLEKMYGDDPGRWDDGL
                     AGAERVRVIVNAMTRLRLCAPDGAMALEFKGEPGDAEQGLTPWFDIPGRLSADHTVIF
                     GHWSALGLTTRPNLLGLDSGCVWGRALSAVSLDDRRILQVRCPRSQGPEG"
     gene            complement(1216..>1843)
                     /locus_tag="VF309_08185"
     CDS             complement(1216..>1843)
                     /locus_tag="VF309_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7466605.1"
                     /translation="VAGAIPHEGDRAAMVAANHVSWLDIFVISSARPTRFIAKSEIRD
                     WPIAGWIAEEAGTLFMRRHEWRDTARTTQRVHAALAGGDCVGLFPEGITTEGDTLLKF
                     HSSLFEAAVGNAAHVYPVAIRYETPHGGPCREVSFVGERTFMQSLALIIRQRAVIVRI
                     DFAEPIDAERATRRDVASQARARVASLLGLEIAGIGPAGSSGRPGSPR"
BASE COUNT          275 a          633 c          658 g          277 t
ORIGIN      
        1 cgggatcatc cttctcgcgc aatttgcggt cggctcccat ggcgcgcgct cgatgaagga
       61 cgatccggcg atgtcgtccg ccgcggtggc caggcgcctc gcgcccgcgg cccaggtcgt
      121 catcgatccg aatgcgcccg cgcccgccgc cgccgcgcct gcctcgacgc cggcgctcgc
      181 gtcggtgacg attcctccgc ctgcggcggc cgcaacgccc tcggccggat ccggcaaggc
      241 gacatactcc agcgtatgca gcgcttgcca cgggaccggg gtggcgggtg ccccgaaact
      301 cggcgacaag gccgcgtggg ccccgcgcat caagaccggc atcgaggcgc tccacgcaag
      361 cgccttgaag gggaaaggcg cgatgcctcc gaagggcggc aatgccgcga tcgcggacgc
      421 cgacgtgaag gccgcagtcg actacatggt cgccgcggcg aagtaacctg gccacctacg
      481 cgatcggcga cctgcagggg tgctacgagc cccttgcgcg gctcgtcgat tcgctgaagt
      541 tcgacccttc gcgcgatcgc ctgtggttcg tcggcgatct cgtcaatcgc gggcccgatt
      601 ccctcgcctg ccttcgctac gtcaagtcgc tcggcgactc cgcggtcacg gtgatcggca
      661 accacgactt tcacctgctg tgcgtcgccg aaggaatcga aaagcgcagg aagcgcgaca
      721 cgatcgacgc ggtcctcgag gcgcccgatc gcgccgagat gctggaatgg ctgcggcacc
      781 gccctctcat gcatgtcgaa gggggattcg cgctagtgca cgcgggactg ctgccgcagt
      841 ggagcgtgcc gcgcgctcgc gcgctcgccg ccgaagtgga atcgcgcttg cgcgcgcccg
      901 gctatcgcgc gctcctcgag aagatgtacg gcgacgatcc cggccggtgg gacgacggcc
      961 tcgcgggcgc cgagcgcgtg cgcgtgatcg tcaatgcgat gacccggctg cggctgtgcg
     1021 cccccgacgg cgcgatggcg ctcgagttca agggcgagcc gggcgacgcg gagcaagggc
     1081 tcacgccgtg gttcgacata cccggacgcc tgagcgccga ccatacggtg atcttcgggc
     1141 actggtcggc gctgggcctc accacgcgcc cgaacctgct cgggctggac agcggctgcg
     1201 tgtggggccg cgcgctcagc gcggtgagcc tggacgaccg gaggatcctg caggtccgat
     1261 gcccgcgatc tcaaggccca gaaggctagc cactcgcgcg cgggcctgtg aggcgacgtc
     1321 gcgccgggtg gcccgctcgg catcgatcgg ctccgcgaaa tcgattctca cgatcaccgc
     1381 gcgctgccgg atgatgagcg cgagcgattg catgaacgtg cgctcgccga cgaaggagac
     1441 ttcgcggcag gggccgccat ggggcgtttc gtagcggatg gcgacgggat agacgtgggc
     1501 cgcgttgccc acggcggcct cgaagagcga gctgtggaac ttgagcagcg tgtcgccctc
     1561 ggtggtgatc ccttcgggaa agaggcccac gcaatcgccg cccgcgagcg cggcgtgaac
     1621 cctttgcgtg gtgcgcgcgg tgtcgcgcca ctcgtggcgg cgcatgaaga gcgtgcccgc
     1681 ttcctcggcg atccatcccg cgatgggcca gtcgcggatc tcgctcttcg cgatgaagcg
     1741 cgtcgggcgg gcgctcgaga tcacgaagat gtcgagccac gagacgtggt tcgcggcgac
     1801 catcgcggcg cgatcgcctt catggggaat tgcgcccgcg acg
//
LOCUS       DASVII010000630         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_424699,
            whole genome shotgun sequence.
ACCESSION   DASVII010000630 DASVII010000000
VERSION     DASVII010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424699"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..957)
                     /locus_tag="VF309_08190"
     CDS             complement(22..957)
                     /locus_tag="VF309_08190"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7466606.1"
                     /translation="MDALSEVLRLARFTAGVTLDATARAPWCVSVSATPAMARAHLVI
                     EGECFLKTGSGEAIALKTGELALLPRGDAHLIGSSLDADARSLSTLVKTPVAGELVPI
                     RLGGDGASTRWIALSASIERHLAEPLIASLPDVMRVDLHNAQALFWLTDALGLTLSAS
                     AAPPVGATAERSRLAELVIIEAISRYVYWLPPGGRGWIAGLNDRYVGRALALVHGRPS
                     EPWTVERLGRQVGLSRSALADHFSEVMGEPIFAFLTRWRLLMAAEYLLTTTRRVESIA
                     HDAGYESAGAFSAAFKRAFGKPPSIWRKRRSGRIG"
     gene            complement(1028..>1833)
                     /locus_tag="VF309_08195"
     CDS             complement(1028..>1833)
                     /locus_tag="VF309_08195"
                     /inference="COORDINATES: protein motif:HMM:NF022514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HEX7466607.1"
                     /translation="QLQAHVLAEGARVTLVPYLYSIDQLPRHFSELRKLAARLAEAAI
                     ANEAPRIDLARLPETAHLLADTRFLVACAAVPRSHAMFRWQEDASGHAGRAGSLEQWI
                     AQARPTISKLLPGCVFECLLPDAYYVNCRESDRRVRPFGVRAAVAFLENALKTKAAEI
                     HAVVAGFGEERVDEYRVAFTRGDEAEVAHGVVWPLYGREDETARPGPLDERLAQRAEC
                     GVSEVKKLPGTFTPEYCEDCGAPLFANGEGEIVHAELPEEADIQAAHFH"
BASE COUNT          287 a          631 c          657 g          258 t
ORIGIN      
        1 gaggcccacg agcgacgccc actacccgat tcgcccgctg cgcctctttc gccagatcga
       61 cggcggcttt ccgaaagcgc gcttgaacgc cgccgagaac gcgcccgcgg attcgtagcc
      121 ggcgtcgtgc gcgatcgatt cgacgcggcg cgtggtggta aggagatact ccgcggccat
      181 cagcaaccgc catcgcgtga ggaacgcgaa gatcggctcg cccatcacct cgctgaagtg
      241 gtcggcgagc gcgcttcgcg acagccccac ctggcgcccg aggcgctcga cggtccaggg
      301 ctccgaggga cgtccatgca ccagcgcgag cgcgcgcccc acgtagcgat cgttgaggcc
      361 ggcgatccag cccctgccgc ccggcggcag ccagtaaacg tagcggctaa tcgcctcgat
      421 gatgacgagc tcggcgaggc gcgagcgttc ggcggtagcg cccaccgggg gcgcggcgct
      481 cgcggagagg gtgaggccga gcgcgtcggt cagccagaag agcgcctgcg cattgtggag
      541 gtccacgcgc atcacgtcgg gaagcgaggc gatgagcggc tcggcgagat ggcgctcgat
      601 cgacgcggag agcgcgatcc atcgcgtgga tgccccgtcg ccgccgaggc gtatcggcac
      661 cagctcgccc gcgacgggcg tcttcacgag agtcgagagc gaccgggcgt cggcgtcgag
      721 ggagctgccg atgaggtgcg cgtccccccg cggcaggagc gcgagctcgc ccgtcttcag
      781 cgcgatcgcc tcgcccgagc ccgtcttcag gaagcactcg ccctcgatca ccaggtgcgc
      841 gcgggccatc gcgggcgtgg ccgagacgga cacgcaccac ggcgcgcgcg ccgtggcgtc
      901 gagcgtcacc ccggccgtga atctagcgag tcgcagaact tccgagaggg catccatgaa
      961 aaactggtgt cagagaccaa tttccgcagc atacggaaat tggtctctga caccagtttt
     1021 ttctgcgtca gtggaagtgg gcggcctgga tgtccgcttc ttcggggagc tcggcgtgga
     1081 cgatctcgcc ctcgccgttg gcgaagaggg gcgcgccgca atcctcgcag tactcggggg
     1141 tgaaggtgcc ggggagcttc ttcacctcgg acacgccgca ttcggcgcgc tgcgcgagcc
     1201 tctcgtcgag cgggccgggg cgcgcggtct cgtcctcgcg cccatagagc ggccagacga
     1261 cgccgtgggc gacctccgcc tcgtcgcccc gcgtgaacgc gacgcgatat tcgtcgacgc
     1321 gctcctcgcc gaagcccgcg acgaccgcgt ggatctcggc cgccttggtc ttcagcgcgt
     1381 tctccaggaa cgccaccgcc gcgcgaacgc cgaacgggcg cacgcgccgg tccgactcgc
     1441 ggcaattcac gtaatacgcg tcgggcagca ggcattcgaa cacgcagccc ggaaggagct
     1501 tcgagatcgt gggacgggcc tgggcgatcc attgctcgag actgcccgcg cgcccggcgt
     1561 ggcccgaggc gtcttcctgc cagcggaaca tcgcatggct gcggggcacc gcggcgcacg
     1621 cgacgaggaa gcgcgtgtcg gcgaggagat gcgcggtctc gggcaggcgc gcgaggtcga
     1681 tgcgcggcgc ttcgttcgcg atggccgctt cggccagccg cgcggcgagc ttgcgcagct
     1741 ccgagaagtg ccgcggcagc tggtcgatcg aatagaggta gggcacgagc gtcacgcgcg
     1801 cgccttccgc gagcacgtgc gcctgcagct gca
//
LOCUS       DASVII010000631         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_65510, whole
            genome shotgun sequence.
ACCESSION   DASVII010000631 DASVII010000000
VERSION     DASVII010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65510"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF309_08200"
     CDS             <1..402
                     /locus_tag="VF309_08200"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7466608.1"
                     /translation="VVAKLADAGILTSVFIDAELAQVEAAARIGARVCEIHTGPYAHA
                     FHSKGRDAEAKPVLAEVDKVRRAGEAIRSLGMRFNAGHALNYANVEPIAALPGIRELH
                     IGHAIVSRAIFTGMREAVREMKALMTNASAR"
     gene            399..788
                     /gene="acpS"
                     /locus_tag="VF309_08205"
     CDS             399..788
                     /gene="acpS"
                     /locus_tag="VF309_08205"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7466609.1"
                     /translation="MIVGIGTDVCSIERIGRSLERFGDRLVKRILTPQERPRFQRTRD
                     KAGHLAKRFAAKEAFAKAIGTGIHAPFTWQSITVTRDAKGKPGIEPDRAMASYLASRG
                     IDSFHISLTDDGGVAMAFVVLEGRARA"
     gene            785..1810
                     /gene="nagZ"
                     /locus_tag="VF309_08210"
     CDS             785..1810
                     /gene="nagZ"
                     /locus_tag="VF309_08210"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX7466610.1"
                     /translation="MRLGPVMVDVAGLELTPDDLERLQHPQVGAVILFARNFAAPLQL
                     IQLAHSIREVRSPQLLIGVDHEGGRVQRFRHGFTAIPAMTELGKLWDRDPAQALAAAR
                     GCGFVIASELQAHGVDFSFTPVLDVDYGESSVIGDRALHHDPHTVAVLAEALQAGLNA
                     AGMSSVGKHFPGHGYVRADSHHEVPIDDRTLTEISATDLLPFQRLARSGMGGMMPAHV
                     VYPKVDSRPAGFSSVWLQRILREKLGFEGLIFSDDLSMEGASTAGGVMARANAALNAG
                     CDMVLLCNDPRAQDTLLEGLERRPIAPTLARRLEKMRGKAISTVALKANAAYLAATEN
                     LARIRSA"
     gene            1817..>2279
                     /locus_tag="VF309_08215"
     CDS             1817..>2279
                     /locus_tag="VF309_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7466611.1"
                     /translation="MAAPFDPAEFVAGLPNLPGVYRFLNADAVAIYVGKAGDLRKRVA
                     SYFQKRDQSPRTAMMISQIAGAETTVTRTEAEALLLENNLIKSLAPRYNVLFRDDKSY
                     GYILVTGHKYPQIRFYRGAHQKGNKYFGPFPSAWSIRETIGHLQKIFKLRTC"
BASE COUNT          376 a          799 c          766 g          338 t
ORIGIN      
        1 gtcgtcgcga agctcgccga cgcgggaatc ctgacgagcg tcttcatcga cgccgagctc
       61 gcgcaggtgg aagccgcggc gcgcatcggc gcgcgcgtct gcgagatcca cacgggcccc
      121 tacgcgcacg cgttccattc gaagggtcgc gacgccgagg cgaaacccgt tctcgcggag
      181 gtcgacaagg tacgccgcgc cggggaggcg atccgttccc tcgggatgcg tttcaacgcg
      241 ggccacgcgc tcaactacgc gaacgtcgag cccatcgcgg cgctccccgg aattcgcgag
      301 ctgcacatcg ggcacgcgat cgtgagccgc gcgatcttca cgggaatgcg cgaggcggtg
      361 cgggagatga aggccctgat gaccaacgcg agcgcgagat gatcgtgggc atcggaaccg
      421 acgtctgctc gatcgagcga atcggcagga gcctcgagag gttcggcgat cgcctcgtca
      481 agcgcatcct cacgccgcag gagcgtccac gcttccagcg cacccgggac aaggccggcc
      541 acctggcgaa gcgcttcgcg gcgaaggagg cgttcgccaa ggcgatcggc accggcatcc
      601 acgcgccctt cacctggcag agcatcacgg tgacgcgcga cgcgaagggc aagccgggaa
      661 tcgagcccga tcgcgcgatg gcgagctacc tcgcgtcgcg cggcatcgat tcgttccaca
      721 tctcgctcac cgacgatggc ggcgtcgcca tggcgttcgt cgtcctcgag ggaagggcgc
      781 gggcatgagg ctcgggccgg tcatggtcga cgtcgcgggc ctcgagctca cgcccgacga
      841 cctcgagcgc ctgcagcatc cgcaggtcgg cgcggtgatc ctcttcgcgc gcaacttcgc
      901 cgcgccgctg cagctgatcc agctcgccca ttcgatccgc gaggtgcgct cgccgcagct
      961 gctcatcggc gtcgaccacg agggcgggcg cgtgcagcgt ttccgccacg gcttcaccgc
     1021 gatcccggcg atgaccgagc tcggcaagct ctgggatcgc gatccggcgc aggcgctcgc
     1081 cgcggcgcgc ggctgcggct tcgtgatcgc gagcgagctg caggcgcacg gcgtcgattt
     1141 cagcttcacc ccggtgctcg acgtcgacta cggcgaatcg agcgtgatcg gcgatcgcgc
     1201 gctgcatcac gatccgcaca ccgtcgccgt gctcgccgag gccctgcagg cgggattgaa
     1261 cgccgcgggg atgtcgagcg tcggcaagca tttccccggc cacggctacg tgcgcgccga
     1321 ttcgcaccac gaagtgccca tcgacgatcg cacgctgacc gagatctcgg cgacggacct
     1381 cctgccgttc cagcgcctgg cgcgctccgg catgggcggc atgatgccgg cgcacgtggt
     1441 ctatccgaaa gtcgattcca ggcccgcggg attctcgagc gtctggctgc agcggatcct
     1501 gcgcgagaag ctcggcttcg aggggctcat cttctccgac gatctttcga tggagggcgc
     1561 gagcaccgcg ggcggcgtga tggcccgcgc caacgcggcg ttgaacgccg gatgcgacat
     1621 ggtcctgctg tgcaacgatc cgcgcgccca ggacacgctc ctcgaagggc tcgagcgccg
     1681 gccgatcgcg ccgacgctcg cgcgccgcct cgagaagatg cggggcaagg cgatctccac
     1741 ggtggcgctg aaggccaacg ccgcgtatct cgcggcgacg gagaacctcg cgcgcatccg
     1801 ctcggcctag tcgagcatgg ccgccccatt cgatcccgcc gagttcgtcg cggggcttcc
     1861 caacctgccg ggcgtctatc gtttcctcaa cgcggacgcg gtggcgatct acgtcggcaa
     1921 ggcgggcgac cttcgcaagc gcgtcgcctc gtacttccag aagcgcgacc agagcccgcg
     1981 caccgcgatg atgatctcgc agatcgcggg ggcggagacc acggtcacgc gcaccgaggc
     2041 cgaggcgctg ctcctcgaga acaacctcat caagagcctc gcgccgcgct acaacgtgct
     2101 tttccgcgac gacaagagct acggctacat cctcgtcacc gggcacaagt atccgcagat
     2161 ccgcttctac cgcggcgcgc accagaaggg caacaagtac ttcgggccgt ttccgagcgc
     2221 gtggtcgatc cgcgagacca tcgggcacct gcagaagatc ttcaagctga gaacctgcg
//
LOCUS       DASVII010000632         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_640342,
            whole genome shotgun sequence.
ACCESSION   DASVII010000632 DASVII010000000
VERSION     DASVII010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640342"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..365
                     /locus_tag="VF309_08220"
     CDS             15..365
                     /locus_tag="VF309_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466612.1"
                     /translation="MQRERFPEKEAPIAYSSDFRIDASLVARACRADAIIMHPLPRDS
                     REGAIDLSSDLDGDSRLAVFRQTDAGIPTRMALFASVMGVAEGIERSFSKAGWYRPAF
                     IGPNDAPFYRQGKA"
     gene            complement(371..1420)
                     /locus_tag="VF309_08225"
     CDS             complement(371..1420)
                     /locus_tag="VF309_08225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX7466613.1"
                     /translation="MERSPRRRALRGALASLLVLAAAPLACAAAPIAAPGAPKDATAA
                     YRNLVDAANAVVTVSMKALPNARSIATLGGERTGSGVAIAPSGLVLTIGYLILEADSV
                     EVTTNAGRTVPATVAAYDHATGFGLLRPVAALGVKPIRMGSSRSVEELDQLMIAAGGG
                     EDSVSVATVVSRRLFAGYWEYLVDSAIFTSPPRADHSGAALINRDGELVGIGSLLVMD
                     AMTPGERLPGNMFVPIDLLKPILDELIATGRQKAGIRPWLGLSSLEEDGRIKVLRVSE
                     GGPAAEAGIGPGDIILMMGGRKVEKLEDFYRRLWSAGAPGIDVNLKVLHGTDIRDVKV
                     HSIDRFEFIRKQPMI"
     gene            complement(1458..>1650)
                     /locus_tag="VF309_08230"
     CDS             complement(1458..>1650)
                     /locus_tag="VF309_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466614.1"
                     /translation="KMRATWSTEPPGGNTAMILTGLFGHAPCEWAASGTNQSKAQANA
                     ANGARDFNIEISGMIRGRV"
BASE COUNT          258 a          553 c          573 g          266 t
ORIGIN      
        1 tctatgcaac gcgagtgcaa agggagcgct ttccggaaaa ggaggcgccg atcgcctatt
       61 cgtccgattt ccgcatcgat gcctcgctcg tggcgcgcgc ttgccgagcg gacgcgatca
      121 tcatgcatcc cctgccgcgc gacagccggg aaggcgcgat cgacctgagc agcgacctgg
      181 acggcgactc gcgcctggcc gtatttcgcc agacggatgc gggcattccc acccgaatgg
      241 cgctcttcgc gagcgtgatg ggcgtcgcgg agggcatcga gcgatcgttc tcgaaagccg
      301 gctggtatcg gccggctttc atcggaccga acgacgcgcc attctaccgg caggggaaag
      361 cctaggctcc ctagatcatc ggctgcttgc ggatgaattc gaagcggtcg atcgagtgca
      421 ccttcacgtc gcggatgtcg gtgccgtgca ggaccttcag gttcacgtcg atgccgggcg
      481 cgccagcgga ccagaggcgc cggtagaagt cctcgagctt ctcgaccttc cggccgccca
      541 tcatgaggat gatgtcgccg gggccgatgc cggcttccgc ggccgggccg ccctcgctca
      601 cgcgcagcac cttgatgcgt ccgtcctctt cgagcgagct caggccgagc cagggacgaa
      661 tgcccgcctt ctgccggccg gtcgcgatga gctcgtcgag gatcggcttc agcaggtcga
      721 tcggcacgaa catgtttccg ggcagccgct cgccgggcgt catcgcatcc atcacgagga
      781 gcgagccgat gccgacgagc tcgccgtcgc gattgatgag cgcggcgccg ctgtgatcgg
      841 cgcgcggcgg cgaggtgaag atggcgctgt cgacgagata ttcccagtag cccgcgaaca
      901 ggcggcgcga cacgaccgtc gcgaccgaga cgctgtcctc gcccccgccg gcggcgatca
      961 tcagctggtc gagctcctcc accgagcgcg agctgcccat gcggatgggc ttcacgccca
     1021 gcgccgcgac ggggcgcagc aatccgaatc ccgtcgcgtg atcgtaggcg gccacggtcg
     1081 cgggcacggt acgtccggca ttcgtggtga cttcgaccga atcggcctcg aggatgagat
     1141 agccgatggt gagcaccagc ccgctgggcg cgatcgccac gccgctgccg gtgcgctcgc
     1201 cgccgagcgt cgcgatgctt cgcgcattgg gaagcgcctt catgctgacg gtcaccacgg
     1261 cgttcgcggc atcgacgaga ttccggtagg cggcggtggc gtccttgggc gcaccgggcg
     1321 cggcgatggg agcggcggcg caggcgaggg gcgccgcggc gagaaccagc agggaagcga
     1381 gcgcgccccg gagggcgcgg cgacgaggcg agcgttccat ggcaacctcc cgcgacgaat
     1441 cagcgagtca tgctagccta cacccgaccc cgaatcatcc cggagatttc aatgttgaag
     1501 tcgcgcgccc cgttcgccgc gttcgcctgc gctttgctct ggttggtccc gctcgcggcc
     1561 cattcgcagg gcgcgtggcc gaacaagccg gtgaggatca tcgcggtgtt tccgcccggt
     1621 ggctcggtcg accaggtggc gcgcatcttc
//
LOCUS       DASVII010000633         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_137394,
            whole genome shotgun sequence.
ACCESSION   DASVII010000633 DASVII010000000
VERSION     DASVII010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137394"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF309_08235"
     CDS             <1..290
                     /locus_tag="VF309_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7466615.1"
                     /translation="GLMFGLPEPLDLKTASAIVLNVDQRMVGLIVSSVSDVVALAPDE
                     ILPAPELADRAIGAAISGIGSRRGALAAEAESSILLLDVDHIMRRVREERG"
     gene            287..1621
                     /locus_tag="VF309_08240"
     CDS             287..1621
                     /locus_tag="VF309_08240"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7466616.1"
                     /translation="MSHKPVYADSPSDPAVPERLGNYEIRGILGRGQSATIYLGRELF
                     PARDVAIKVYDAHQLDPEDRKVFRSLFLKETLLARKLTHPNITQVYDAAADDDRAYIV
                     MEYAQEGSLDRYCVPEGMLKPERVAHLLEHVCDALSYAHANGIIHRDLKPANVLMSAE
                     GEAKVADFGVAFSNFAFDTTRGMNVGSPAYMAPEQFERKPASMQTDVYSVGIMMYKML
                     TGALPFPLDTPAALTTRILLGNFPPPSAARPGLPPVFDAIFRRSTARDASNRYRTWEE
                     FASDLRTVSQPGADMADADQRVATMKSLPFFRDFDTACLAEAAPMARWFDVRAGSQLV
                     GEDDPGYSFFVLVRGQMRITRHGTLIAIRGAGECLAEIAFLRRSGARRFSSIAAVTDC
                     TVMEFDPDVLWLASPECTRNFHQAFLRAMADRLVAAEGALAEMLGGKSVTLF"
BASE COUNT          264 a          598 c          522 g          270 t
ORIGIN      
        1 tgggcctcat gttcggactt ccggagccgc tggatctcaa gaccgcgagc gcgatcgtgc
       61 tgaacgtcga ccagcgcatg gtggggctca tcgtctcgag cgtatccgat gtggtcgcgc
      121 tcgcgcccga cgagatcctg cccgcccccg agctggcgga tcgcgcgatc ggcgcggcca
      181 tatccggcat cggatcgcgc cgcggcgccc tggccgccga ggccgaatcg agcatcctcc
      241 tcctcgacgt cgatcacatc atgcggcgcg tccgcgagga gcgcgggtga gccacaagcc
      301 ggtctacgcg gactccccga gcgaccccgc ggtgccggag cgcctcggca actacgagat
      361 ccgcggaatc ctcggtcgcg gccagtcggc gaccatctac ctgggccggg agctctttcc
      421 ggcgcgcgac gtcgccatca aggtctacga cgcgcatcag ctcgatcccg aggatcgcaa
      481 ggtgttccgc tcgctcttcc tcaaggagac gctcctcgcg aggaagctca cgcatcccaa
      541 catcacccag gtgtacgacg cggccgccga cgacgatcgc gcctacatcg tgatggaata
      601 cgcgcaggaa ggcagcctcg accgctattg cgtccccgag ggaatgctca agcccgagcg
      661 cgtcgcgcac ctgctcgagc acgtgtgcga cgccctgagc tatgcccacg ccaacggcat
      721 catccaccgc gacctgaaac ccgccaacgt gctcatgagc gccgagggcg aggcgaaggt
      781 cgcggacttc ggcgtcgcgt tctcgaattt cgcgttcgac acgacacgcg ggatgaacgt
      841 gggctcgccg gcctacatgg cgcccgagca gttcgaaagg aagcccgcgt cgatgcagac
      901 cgacgtctac tcggtgggca tcatgatgta caagatgctc acgggcgcgc tgccgtttcc
      961 cctggacacg cccgcggcgc tgacgacgcg catccttctc ggaaacttcc cgccgccttc
     1021 ggcggcgcgc ccgggccttc ccccggtctt cgacgccatc ttccgccgct ccacggcgcg
     1081 cgatgcgtcg aaccggtatc gcacgtggga agaattcgcc tcggacctgc gcaccgtctc
     1141 gcagcccggc gcggacatgg ccgatgccga ccagcgtgtg gcgaccatga aatcgctgcc
     1201 gttcttccgc gacttcgaca cggcgtgcct cgccgaggcg gcgccgatgg cgcgctggtt
     1261 cgacgtgcgc gcgggctcgc aactggtggg cgaggacgac cccggttatt cctttttcgt
     1321 gctcgtgcgc ggccagatgc gcatcacgag gcacggaacg ctcattgcga tccgcggcgc
     1381 gggcgaatgc ctcgcggaga tcgccttcct gcgccgctcc ggcgcgcgcc gcttcagctc
     1441 catcgccgcg gtcaccgatt gcaccgtgat ggaattcgat ccggacgtcc tctggctcgc
     1501 ctcgcccgag tgcacccgca acttccacca ggcgttcctt cgcgcgatgg cggaccggct
     1561 cgtcgccgcc gagggggcgc tcgccgaaat gctcggcgga aagagcgtga ccctcttctg
     1621 atcccgccta ttacatgaaa gccaacagtc tgca
//
LOCUS       DASVII010000634         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_676654,
            whole genome shotgun sequence.
ACCESSION   DASVII010000634 DASVII010000000
VERSION     DASVII010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676654"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1692)
                     /locus_tag="VF309_08245"
     CDS             complement(<1..>1692)
                     /locus_tag="VF309_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7466617.1"
                     /translation="LELSLGCHYRIAKTDAPLALPEVKLGLIPGAGGTQRLPRAVGVD
                     AALNMITTGAPVPAAKFKGTALINEVIEGDMLEGALRFARQVAGKPARLVRDIAIELP
                     DAPAYFEAARKTVAAASKGLTAPLKCVEAIEAAVALPFDEGIRRERDIFTELIQSTES
                     HALRHAFFGERAASKIPDVPADTPTRRIERVAVIGAGTMGGGITMNFLSAGIPVTLLE
                     MKQEALDKGVATIRRNYEGSVKRGKLTPEKLDKAMALLAPTLAYDDIKGADLVIEAVF
                     EEMGVKEGVFRKLDEVMKPGAILATNTSTLDVDKIAAFTKRPQDVVGLHFFSPANIMK
                     LLEVVRGAKTAKDVMATVMGLAKKIRKTAVVSGVCDGFIGNRMLERYQRQCFYLIEEG
                     ATPQQVDRALENFGFAMGLFRVGDLAGNDIGWAVRKRRYVEHPEVPYSKIADRLCEMG
                     RFGQKTGAGWYRYEPGKRDPIPDPAVDAIIDAYRKEMGIKPRAISDDEIVDRCVYALA
                     NEGARILEEGIAQRASDIDMVYLTGYGFPLKRGGPMFHADTVGLERVVAAMKRFAA"
BASE COUNT          249 a          584 c          564 g          295 t
ORIGIN      
        1 gcggcgaaac gcttcatcgc cgcgaccacc ctctcgagac ccacggtgtc ggcatggaac
       61 atcggcccgc cgcgcttcag cgggaagccg tatcccgtga ggtacaccat gtcgatgtcg
      121 gaggcgcgct gcgcgatgcc ctcctcgagg atgcgcgcgc cttcgttcgc gagggcgtag
      181 acgcagcggt cgacgatctc gtcgtcggat atcgcgcgcg gcttgatgcc catctccttg
      241 cggtaggcgt cgatgatcgc gtccaccgcg ggatcgggaa tcgggtcgcg cttgccgggc
      301 tcgtagcgat accagcccgc gccggtcttc tgcccgaagc gccccatctc gcagaggcga
      361 tcggcgatct tcgagtacgg cacctcggga tgctcgacgt agcggcgctt cctcaccgcc
      421 cagccgatgt cgttgccggc gaggtcgccc acgcggaaga ggcccatcgc gaacccgaaa
      481 ttctccaacg ctcgatcgac ctgctgcggc gtggcgccct cctcgatgag atagaagcac
      541 tggcgctggt agcgctcgag catgcggttg ccgatgaatc cgtcgcagac gccggagacg
      601 acggcggtct tgcggatctt cttcgccagc cccatcacgg tggccatcac gtccttcgcg
      661 gtcttcgcgc cgcgcacgac ctccagcagc ttcatgatgt tcgccgggct gaagaagtgc
      721 aggccgacga catcctgggg gcgcttggtg aaggccgcga tcttgtccac gtcgagcgtt
      781 gaagtattgg tggcgaggat cgcgccgggc ttcatcacct cgtccagctt gcggaacacg
      841 ccttccttca cgcccatctc ctcgaacacc gcctcgatca ccaggtccgc gcccttgatg
      901 tcgtcgtagg cgagcgtcgg cgcgagaagc gccatcgcct tgtcgagctt ttccggcgtg
      961 agcttgccgc gcttcaccga gccctcgtag ttgcggcgga tcgtcgccac gcccttgtcg
     1021 agcgcttcct gcttcatctc gaggagcgtc acgggaatgc cggcgctcag gaagttcatc
     1081 gtgatgccgc cgcccatggt gcccgcgccg atcacggcga cgcgctcgat cctgcgcgtc
     1141 ggcgtatcgg cgggcacgtc gggaatcttc gacgcggcgc gctcgccgaa gaacgcgtgc
     1201 cgcaacgcgt gggactcggt cgactggatg agctcggtga agatgtcgcg ctcgcgccgg
     1261 atgccttcgt cgaaaggcaa ggcgaccgcg gcctcgatcg cctcgacgca cttcagcggc
     1321 gccgtgagcc ccttcgaggc ggccgcgacc gtcttgcgcg cggcttcgaa gtacgcgggc
     1381 gcatcgggca gctcgatcgc gatgtcgcga accaggcggg cgggcttgcc cgcaacctgg
     1441 cgcgcgaagc gcagcgcccc ctcgagcatg tcgccctcga tcacctcgtt gatgagcgcc
     1501 gtgcccttga atttcgccgc gggcaccggc gccccggtgg tgatcatgtt cagcgcggcg
     1561 tccacgccca cggcgcgcgg caggcgctgg gtgccgcccg cgccgggaat gagccctagc
     1621 ttcacttcgg gaagcgcgag cggcgcgtcg gtcttcgcga tgcggtagtg gcagccgagc
     1681 gaaagctcga gg
//
LOCUS       DASVII010000635         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_65606, whole
            genome shotgun sequence.
ACCESSION   DASVII010000635 DASVII010000000
VERSION     DASVII010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65606"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VF309_08250"
     CDS             complement(<1..548)
                     /locus_tag="VF309_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7466618.1"
                     /translation="MTSSLPRSAQKDFERAVSLFQQGRLAIAEGICGELFARFPADAE
                     LAHFAGVVATRMGKFGLAAERLGRSVRLEPARARAQAALGFSLDQVGRVEEARRAFQA
                     AVSLEPGFADAHNGLGITCYRLGLLDEAMAAFSQALALAPESVETRLNAARALMQTGR
                     LEGAARLYREALQRSAARADALP"
     gene            complement(591..2060)
                     /locus_tag="VF309_08255"
     CDS             complement(591..2060)
                     /locus_tag="VF309_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7466619.1"
                     /translation="MNAPYARRNDPCPCGSGARYKDCHGKLGAAPAALGADEAFQRAM
                     EAHRRRQPQAAIALFDVALAADPGNAFALHFKGYGLCQLGRFDEGLPLLERALAAQPR
                     NADFHGNLGIIHYVLGDLARAIPAFERAIALAPRMAEAYSNLAMARRDAGDPERALAD
                     VRRALELNPQLPAARLNYAMILLALGRFAEAWPAYVWRPQPAANLRDLGIAATLPHAT
                     ALPDLTRDPWITLHGEQGLGDALFFLRYAAALRARGARLRFWGDARLAPMLLRSAIVD
                     DATSGGPPPGLDPARLAWIGDLPALLHLESEFPAPVRLAADPARVAAMRERLAAFGPA
                     PYIALTWRAGLPRRGKAVLAKEIDPRALGAALAGLDATFISIQRDPLEGEREALAAAL
                     GAPVGDFSAANADLDDALALLDVVDEYAGVSNTNTHLRASLGRGAHVLVPWPPEWRWL
                     RAGGASPWFPGFPLYRASSTGDWAPALARLRAGFQPVSR"
     gene            2126..>2985
                     /gene="rfaQ"
                     /locus_tag="VF309_08260"
     CDS             2126..>2985
                     /gene="rfaQ"
                     /locus_tag="VF309_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HEX7466620.1"
                     /translation="MVPDAVNLTTVQRVLVVKLRHHGDVLLASPVFHVLKQRAPHAEI
                     DALVYSDTREMLAGHPAIAQIHAVDRAWKRQGLATQARRELDLLKTLQGRRYQFLVHL
                     TDHWRGAWLAQALRPRWSVAPARDSRFWNWSFTHRYKLPRATPRHAVEANLDALRRAG
                     IYPEESEKPLVMVPGDEAVKRVDALLAAASLAPQGFIQVHPASRWLFKTWTESRNAEM
                     LRALYRDGNRIVVTGAPDPRERAIVARIVAEAAVPVLDLSGQLTLRELAALSARARLF
                     FGVDSAPMHIA"
BASE COUNT          438 a         1070 c         1095 g          382 t
ORIGIN      
        1 ggcagcgcgt cggcgcgcgc ggcgctgcgc tgcaaagcct cgcgatagag gcgcgcggcg
       61 ccttccaggc gcccggtctg catcagcgcg cgcgcggcgt tgaggcgcgt ctccacggat
      121 tccggagcga gcgcgagggc ctgcgagaac gccgccatcg cctcgtcgag gagacccagc
      181 cgatagcacg tgatgccgag gccgttgtgc gcgtcggcga accccggctc gaggctcacc
      241 gcggcctgga acgcgcggcg cgcctcctcg acgcggccga cctggtccag cgagaagccg
      301 agtgccgcct gcgcgcgggc gcgcgcgggc tccaggcgca ccgagcgccc gagacgctcc
      361 gccgcgagac cgaacttacc catgcgcgtg gccacgacgc cggcgaaatg cgcgagctcc
      421 gcgtccgcgg gaaagcgcgc gaagagctca ccgcagatgc cttcggcgat ggcgagacgt
      481 ccttgctgga agagggagac cgcgcgttcg aagtccttct gcgcggaccg gggcaagctc
      541 gaagtcatgg ggaaccgcgg ccggaaaagc cgggtgaatt tccgtcgatt ctatcgcgaa
      601 accggctgga acccggccct gaggcgcgcc agcgccggcg cccagtcgcc cgtcgagctc
      661 gcgcgataga ggggaaatcc ggggaaccac ggcgatgcgc cgcccgcgcg gagccagcgc
      721 cattccggcg gccagggcac gagcacatgg gcgccgcgcc cgagggatgc gcgcaggtgc
      781 gtgttcgtat tgctcactcc ggcgtactcg tcgaccacgt cgagaagcgc cagcgcgtcg
      841 tcgagatccg cgttcgccgc ggagaagtcg cccaccggcg cgccgagcgc ggcggcaagc
      901 gcctcgcgtt cgccctcgag cgggtcgcgt tgaatggaga tgaacgtggc atcgagcccg
      961 gcaagcgccg cgccgagcgc gcgcgggtcg atctctttcg cgagcacggc cttgccgcga
     1021 cgcggaaggc ccgcgcgcca cgtgagcgcg atgtacggcg cggggccgaa ggccgcgagg
     1081 cgctcgcgca tcgccgcgac gcgcgcggga tcggcggcga gccgcaccgg cgcggggaac
     1141 tcgctctcga ggtgaagcaa tgcgggcagg tcgccgatcc atgccaaccg cgccggatcg
     1201 aggccgggcg gcggtcctcc cgatgtcgcg tcgtcgacga tggcgctgcg tagcagcatg
     1261 ggcgcgaggc gcgcgtcgcc ccagaagcgc agcctcgccc cgcgcgcgcg caacgccgcg
     1321 gcgtaacgca ggaagaagag cgcgtcgccg aggccctgct cgccgtgcaa cgtgatccac
     1381 ggatcgcgcg tcaaatcggg aagcgccgtg gcgtgcggga gggtcgcggc gatccccagg
     1441 tcgcgcaggt tcgcggcggg ctgtggcctc cacacataag caggccacgc ctccgcgaag
     1501 cgccccagcg cgaggaggat catcgcgtag ttcaggcgcg cggcgggcag ctggggattg
     1561 agctcgagcg cgcggcgcac gtcagccagc gcgcgctcgg gatcgccggc gtcgcgccgc
     1621 gccatcgcga ggttgctgta cgcctccgcc atcctcgggg cgagagcgat cgcgcgctcg
     1681 aaagcgggaa tggcgcgcgc gaggtcgccc agcacgtagt ggatgatgcc gaggttgccg
     1741 tggaagtcgg cgttgcgcgg ctgcgcggcg agggcgcgct cgagaagcgg caggccctcg
     1801 tcgaaacgtc cgagctggca cagcccgtag cccttgaagt gcagcgcgaa cgcgttgccc
     1861 ggatcggcgg caagcgccac gtcgaacaag gcgatcgcgg cctgcggctg gcgccggcga
     1921 tgcgcctcca tggcgcgctg gaacgcctcg tcggcgccga gcgccgcggg ggcggcgccg
     1981 agcttgccgt ggcagtcctt gtagcgcgcg ccgctgccgc aggggcaggg gtcgttgcgg
     2041 cgggcgtagg gcgcgttcat cgtggaaatc gattatccta cccgagcctc cagagccggc
     2101 gattccgccg cgtcatctac cctccttggt ccccgacgcc gtcaacctca ccaccgtgca
     2161 gcgcgtgctg gtggtgaagc tgcgccatca cggcgacgtg ctgctggctt cgcccgtctt
     2221 ccacgtgctc aagcagcgcg cgccgcacgc cgagatcgac gccctcgtct actccgatac
     2281 gcgggaaatg ctcgcggggc atccggcgat cgcgcagatc cacgccgtgg accgcgcgtg
     2341 gaagcgccag ggactcgcca cgcaggcgcg ccgcgagctc gacctgctga aaacgctgca
     2401 gggccggcgc taccagttcc tcgtgcacct caccgaccac tggcgcggcg cgtggctcgc
     2461 gcaggcgctg cgcccgcgtt ggtcggtggc cccggcgcgc gacagccgct tctggaactg
     2521 gagcttcacg caccgctaca agctgccgcg cgcgacaccg cgccatgccg tcgaagccaa
     2581 cctcgacgcg ctgcgccgcg ccggcatcta cccggaggag agcgaaaagc cgctggtgat
     2641 ggtgccggga gacgaggccg tgaagcgcgt cgatgcgctc ctcgcggccg cctcgctggc
     2701 gccgcaggga ttcatccagg tgcacccggc ctcgcgctgg ctcttcaaga cgtggaccga
     2761 gtcgaggaac gccgagatgc tgcgcgcgct ctatcgcgac ggcaatcgca tcgtcgtcac
     2821 cggcgcgccc gacccgcgcg agcgcgccat cgtcgcgcgc atcgtggccg aagccgcggt
     2881 gcccgtgctc gatctctccg gccagctcac gctacgcgag ctggcggcgc tctccgcgcg
     2941 cgcgcgtctt ttcttcggcg tggactccgc gccgatgcac atcgc
//
LOCUS       DASVII010000636         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_460806,
            whole genome shotgun sequence.
ACCESSION   DASVII010000636 DASVII010000000
VERSION     DASVII010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460806"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VF309_08265"
     CDS             complement(<1..898)
                     /locus_tag="VF309_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7466621.1"
                     /translation="MKLEHIRNFCIVAHIDHGKSTLADRLIEATGMLQKREMKAQVLD
                     TLDLERERGITIKLNAVRMTYTARDGQEFELNLIDTPGHVDFTYEVSRSLAACEGAIL
                     VVDASQGIEAQTLSNLFLAMDAGLEIIPILNKIDLPGAEPEKRRKEVADLLGIEESEI
                     LLVSAKEGIGIGELLEEVVRKVPAPEGDETAPLRALIYDSYYDKYRGAIPSVRVVDGV
                     IKKGTHITFGASESVYEVDEVGYNQLRQVPTDQLGPGEVGYVVASVRSVSETRAGDTI
                     FDANNRAAEALPGYQEVRSFVFA"
BASE COUNT          261 a          501 c          533 g          319 t
ORIGIN      
        1 cggcgaagac gaaggagcgc acctcctgat atcccggcag tgcctcggcc gcgcggttat
       61 tcgcgtcgaa gatcgtgtcg ccggcgcgcg tctcgctcac cgagcgcacg ctggccacga
      121 cgtatcctac ttctcccgga cccagttgat cggtgggaac ctgtcgcagc tgattgtatc
      181 cgacctcatc gacctcgtag accgattcgc tcgcgccgaa ggtgatgtgc gtgccctttt
      241 ttatcacgcc gtcgacgacg cgcacgctgg ggatagctcc gcgatacttg tcgtagtacg
      301 agtcgtagat cagcgcgcga agtggagctg tctcgtcgcc ttcgggcgcg ggcaccttgc
      361 gcacgacctc ctcgagcagc tcgccgattc cgattccttc cttcgcgctg acgagaagaa
      421 tctcactctc ctcgatgccg agtagatccg ccacttcctt gcggcgcttc tccggctcgg
      481 cgccggggag atcgatcttg ttgagaatgg ggatgatctc caggcccgcg tccatcgcca
      541 ggaagaggtt ggagagagtc tgggcctcga ttccctggga cgcgtcaacg acgagaatcg
      601 cgccctcgca cgcggcgagc gaccgcgaga cttcgtaggt gaaatcgacg tgccccggag
      661 tgtcaatgag gttgagctcg aactcctgcc cgtctcgcgc ggtgtatgtc atccgaacgg
      721 cgttgagctt gatcgttatc ccacgctcgc gctcgaggtc cagggtatcg agcacttggg
      781 ccttcatctc tcgcttctgc agcatgcccg tagcctcgat gagcctgtcg gcgagggtgg
      841 acttgccgtg gtcgatgtgt gcgacgatgc agaagttacg gatgtgctcg agcttcaacg
      901 ggtgtctctt gggttgtgcg gggagcggcg gaaggaacat cgtaatataa ccgtcactac
      961 ctccttctcc ggccgccgga aaaagaaaag agggctgctt tcgcagccct ctttacgatg
     1021 tcttcgcgcc tacgcgacta ctgtctggat ggcgcgggtt tgagcggctc cgtcctcgta
     1081 accggcggtg gtgacgaagg cgcctggcgc acaactggag ctggcggagg cggaggcgga
     1141 gattgtctgg gcgttgcatc cggaacgcgt gacggcggcg gcggcgctga gcgcgtctcc
     1201 ggcctggact cgcgaacagg ctgcgtgccc tgcgtgccgc tcgatgccgc aggttcctgg
     1261 cgcggcgtcg tgacgcgctc taccgggacc cggccctgcg gggccgagct cgttcccgac
     1321 gacgggcgat agccttgaag aatctgcccg ggctcaccgc gacgtccttt gggagccgta
     1381 atcgtcacgt cgcccatctg aggaagaccc tcgcgctgcg gcagcgggaa gattcgctgc
     1441 ggcgccgctt tcgatccgga ctcgatcggg tgacgaggcg tgccgtgcgg caaccggcca
     1501 atggggatga cgctgctcgt gtctcccgga gaccgtggcg gtcgcacgcc cgggaagtgg
     1561 cccgctactg agtgcgaccg gactatcacg tacgggaatc cacaaaggtc gtag
//
LOCUS       DASVII010000637         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_353242,
            whole genome shotgun sequence.
ACCESSION   DASVII010000637 DASVII010000000
VERSION     DASVII010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353242"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..679
                     /gene="purE"
                     /locus_tag="VF309_08270"
     CDS             182..679
                     /gene="purE"
                     /locus_tag="VF309_08270"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7466622.1"
                     /translation="MDEKPKVGVVMGSQSDWDVMQHAAKVLKDFGVPFEHLVVSAHRT
                     PDTMFEYAEEARGRGLACIIAGAGGAAHLPGMIAAKTTLPVLGVPVPSRHLAGQDSLY
                     SIVQMPKGIPVATFAIGEAGAANAGLFAVSLLAASDPALATKLAEYREKMREQVAAMQ
                     LPDVL"
     gene            717..1862
                     /locus_tag="VF309_08275"
     CDS             717..1862
                     /locus_tag="VF309_08275"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7466623.1"
                     /translation="MILPGATLGLLGGGQLGRMFTVAARTLGYRVTVLDPDPLSPAAE
                     FATRHLATAYTDPAAIAELASSCAAVTTEFENAPAEALDAIAARTVVRPGGFAVAVAQ
                     DRRREKAFLAERGFPVGSFATIDTPGDIGPALARVRLPALLKTARFGYDGKGQARIES
                     AEDLERTLAQWKSVPCVLEELLALEREVSVILARSDDGAIATFPVAENLHARGILDLS
                     IAPARVAPALAEEAASLASRLATALDYVGVIAVEMFVVGGRLLLNEIAPRPHNSGHYT
                     IDACRTSQFEQQVRVLCGMPLGDPSQHTPAVMVNLLGDIWRDGEPRWEAVLRHSGAHL
                     HLYGKRDARPGRKMGHVTVCETTPERTLEVALDIRRALGIGPAGESV"
     gene            1908..>2284
                     /locus_tag="VF309_08280"
     CDS             1908..>2284
                     /locus_tag="VF309_08280"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX7466624.1"
                     /translation="MNTFRRIVAPFALMSAFLAFAAMAADKRETRPVSGFTGIALAAP
                     INVELVQGDTESLVLEGDEATLAEIETVVEQGVLKIRTRSSFDSRHLSKARALVGAKA
                     IESLRIAGAGDIKAALLRSADLKL"
BASE COUNT          347 a          808 c          795 g          334 t
ORIGIN      
        1 tacggcaagg gctaccagga cgtgcagaac cgggtgccga agatcgacaa caccatggcc
       61 gagctcggct ggtcgccgcg agtcgacatg aagaccgcgc tcggccacat cttcaacgcc
      121 tatcgcggcc agctcgaaga cgcgcgcgcg cttggcggct agactaggcg agatggaaac
      181 gatggacgaa aaaccgaagg tcggcgtggt gatgggctcc cagagcgact gggacgtgat
      241 gcagcacgcc gccaaggtgc tcaaggattt cggggtgccg ttcgagcatc tcgtggtgtc
      301 ggcccatcgc acgcccgaca cgatgttcga gtacgccgag gaagcgcgcg ggcgcggcct
      361 cgcgtgcatc atcgcgggcg cgggcggcgc ggcgcacctt cccggcatga tcgccgccaa
      421 gaccaccctc cccgtccttg gcgtgcccgt gccctcccgg catctcgcgg gccaggattc
      481 gctctactcg atcgtgcaga tgccgaaggg gattccggtc gcgacgttcg cgatcggaga
      541 ggccggcgcc gccaacgcgg gactcttcgc cgtgagcctc ctggccgcgt cggatccggc
      601 gctcgcgacg aagctcgccg aatatcgcga gaagatgcgc gagcaggtcg cggcgatgca
      661 gctgccggat gtgctctgat gtcgcgtcga ccggcgcggc ggcgacgcga agtgctgtga
      721 tcctgccggg cgcgacgctg gggctcctcg gcggcgggca gctcgggcgc atgttcacgg
      781 tcgccgcgcg cacgctgggc tatcgcgtga cggtgctcga tcccgatccc ctatctcccg
      841 cggccgaatt cgccacgcgc cacctcgcga ccgcatacac cgatccggcg gcgatcgcgg
      901 agctcgcgtc gagctgcgcg gcagtcacca cggagttcga gaacgctccc gccgaggcgc
      961 tcgatgcgat cgccgcgcgc accgtggttc gccccggcgg cttcgcggtc gcggtggcgc
     1021 aggaccgccg ccgcgagaag gcgttcttgg ccgagcgcgg cttcccggtg ggaagcttcg
     1081 cgacaatcga tacgcccggg gacatcgggc ccgcgctcgc gcgcgtgcgc cttccggcgc
     1141 tgctcaagac ggcgcgcttc ggctacgacg gcaaggggca ggcgcgcatc gagtccgcgg
     1201 aagatctcga gcggacgctc gcgcagtgga agtccgtgcc gtgcgtgctc gaggagctcc
     1261 tcgccctgga gcgcgaggtc tcggtgatcc tcgcgcgaag cgacgacggc gcgatcgcga
     1321 catttccggt cgcggagaac ctccatgcgc gcggaattct cgacctctcg atcgcgcccg
     1381 cgcgcgttgc gccggcgctc gccgaggagg ccgccagcct cgcctcgcgc ctggccaccg
     1441 cgctcgacta cgtcggcgtg atcgcggtcg agatgttcgt cgtcggcggg cggctcctgc
     1501 tgaacgagat cgcgccgcgc ccccacaaca gcggccacta cacgatcgac gcctgccgca
     1561 cctcgcaatt cgagcagcag gtgcgcgtgc tctgcgggat gcccctcggc gatccctcgc
     1621 agcacacgcc cgcggtgatg gtgaacctcc tcggcgacat ctggcgcgac ggcgagccgc
     1681 gctgggaagc ggtgctgcgc cactccggcg cgcaccttca cctgtacgga aagcgcgacg
     1741 cgcgtcccgg gcgcaagatg ggccatgtca cggtctgcga gacgaccccc gagcgaaccc
     1801 tcgaggtggc cctcgacatc cggcgcgccc tgggaatcgg cccggccggt gaatccgtct
     1861 agcgccctgc cgcatcgaag cggtatcgtg acccgcagga ggcttccgtg aacacatttc
     1921 gccgcatcgt cgccccgttc gccctgatga gcgctttcct cgcgttcgcc gccatggccg
     1981 cggacaagcg agagacccgg ccggtctcgg ggttcaccgg catcgccctc gcggcgccga
     2041 tcaacgtcga gctcgtgcag ggagacaccg agagcctcgt cctcgaaggc gacgaggcca
     2101 cgctcgccga gatcgagacc gtcgtcgagc aaggcgtgct gaagatccgg acgcgttcga
     2161 gcttcgattc gcggcacctg tcgaaggcgc gcgcgctcgt cggggcgaag gcgatcgaat
     2221 cgctgcgaat cgcgggcgcc ggcgacatca aggccgcgct gctgcgctcg gcggacctca
     2281 agct
//
LOCUS       DASVII010000638         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_640615,
            whole genome shotgun sequence.
ACCESSION   DASVII010000638 DASVII010000000
VERSION     DASVII010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640615"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF309_08285"
     CDS             complement(<1..550)
                     /locus_tag="VF309_08285"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7466625.1"
                     /translation="MRRLAAAVGLAAWCGLALAAPRSPHEELTLEIYRELVEINTVHP
                     DGDNTAAARAMARRLLDAGFDAADVQVHEPAPRKGNLVARYRGTGELRPILLLAHIDV
                     VEARKEDWSDGLDPFRLTERDGYYYGRGTLDDKAMAAIFVANLIRYRKEGLRPKRDII
                     VALTADEETGGHNGVAFLLKNRR"
     gene            complement(547..933)
                     /locus_tag="VF309_08290"
     CDS             complement(547..933)
                     /locus_tag="VF309_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466626.1"
                     /translation="MKIGKLLGVVALSTAACTLAHAQVTCAECKSTALAEASQCRAQA
                     APDPALLAQCDKKYAEMGLGCQETACRADAGASAAAQCSDCVKQAEAEVKKCAQLPRD
                     VRLACEARVASEKKSCEDKSCPAAKK"
     gene            complement(956..>2257)
                     /gene="ffh"
                     /locus_tag="VF309_08295"
     CDS             complement(956..>2257)
                     /gene="ffh"
                     /locus_tag="VF309_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7466627.1"
                     /translation="GQSRLTEDNVQEMLREVRLALLDADAGVPVVKDFIAKVKERALG
                     QEVAASLTPGQALVGIVHHELVDLMGGSNAALSFATQPPAVILLAGLQGSGKTTSAGK
                     LARLIKNTTKKKVLLVSADVYRPAAIEQLATLAKSLEIELYPSDPSQKPVDIARAALD
                     HAKRHFFDVLIVDTAGRLAIDEAMMAEVKALHAALNPIETLFVVDAMQGQDAVNVARA
                     FSEALPLTGIILTKLDGDSRGGAALSVRHVTGKPIKFVGVSEKMDGLEAFHPDRMASR
                     ILGMGDILSLVEEAHAKVDAKEAQKLADKFKKGKDFDLEDFKTQLTQMKKMGGIASLS
                     DKLPAQLTNMAQASPELQEKALKRTEAIINSMTPLERRKPELLKASRKRRIATGAGVQ
                     VQEVNRLLAQFEQMQKMMKMMRGGNLGKMLRNMKGMFPGGR"
BASE COUNT          302 a          782 c          763 g          410 t
ORIGIN      
        1 cgcggcggtt cttcagcagg aacgccacgc cgttgtggcc gccggtttcc tcgtcggcgg
       61 tgagggcgac gatgatgtcg cgcttcggcc gcaagccctc cttgcggtag cgaatgaggt
      121 tcgccacgaa gatcgccgcc atcgccttgt cgtcgagcgt gccgcggccg tagtagtagc
      181 cgtcgcgctc ggtgagcctg aagggatcga gcccgtcgga ccaatcctcc ttcctcgcct
      241 cgaccacgtc gatgtgtgcg aggaggagga tcggacgcag ctcgcccgtg ccgcgatagc
      301 gcgcgacgag attgcccttg cgcggggcgg gctcgtggac ctgcacgtcc gccgcgtcga
      361 agcccgcgtc gaggaggcgc ctggccatcg cgcgcgcggc cgcggtgttg tcgccgtcgg
      421 ggtggacggt gttgatctcg accagctcgc ggtagatctc gagagtgagc tcctcgtgag
      481 gcgagcgtgg cgcggcgagc gcgaggccgc accatgcggc gagcccgacg gcggcggcga
      541 gccgcctcac ttcttcgccg cggggcagga cttgtcctcg caggatttct tctcgctcgc
      601 gacgcgtgcc tcgcaggcga ggcgcacgtc cctaggcagc tgcgcgcatt tcttcacctc
      661 ggcctcggcc tgcttcacgc aatcggagca ctgcgccgcc gcggaggccc cggcgtcggc
      721 gcggcacgcg gtctcctggc aaccgaggcc catctccgcg tacttcttgt cgcactgggc
      781 gaggagcgcc ggatcgggcg cggcctgcgc gcggcactgg ctcgcctcgg cgagcgcggt
      841 ggatttgcat tccgcgcagg tcacctgcgc gtgcgcgagc gtgcacgccg ccgtcgacag
      901 cgccacaacg cccagcagtt tcccgatctt catcgcgaat cccctcgcgc aatgatcaac
      961 gcccgcccgg aaacatcccc ttcatgttgc gcagcatctt gccgagattg ccgccgcgca
     1021 tcatcttcat cattttctgc atctgctcga actgggcgag gaggcggttc acctcctgca
     1081 cctgcacgcc cgcgccggtg gcgatgcggc gcttgcgcga ggccttgagg agctcgggct
     1141 tgcgccgctc gaggggcgtc atcgaattga tgatcgcttc ggtgcgcttc agcgccttct
     1201 cctgcagctc gggcgaggcc tgcgccatgt tggtgagctg cgccggcagc ttgtccgaga
     1261 ggctcgcgat gccgcccatc ttcttcatct gggtgagctg cgtcttgaag tcctcgaggt
     1321 cgaagtcctt gcccttcttg aacttgtcgg cgagcttctg cgcctccttg gcgtcgacct
     1381 tggcgtgcgc ctcctcgacc agcgagagga tgtcgcccat gccgaggatg cgcgaggcca
     1441 tgcggtccgg gtggaaggcc tccaggccgt ccatcttttc cgacacgccg acgaacttga
     1501 tcggcttgcc ggtcacgtgc cgcaccgaga gcgccgcgcc gccgcgcgaa tcgccgtcca
     1561 gcttggtgag gatgatgccg gtcaacggca gcgcctcgct gaaggcgcgc gcgacgttca
     1621 ccgcgtcctg gccctgcatc gcgtcgacca cgaagagcgt ctcgatcggg ttcagcgcgg
     1681 catgcaacgc cttcacctcc gccatcatcg cctcgtcgat ggccaggcga cccgccgtat
     1741 cgacgatgag gacgtcgaag aagtggcgct tcgcgtggtc gagcgccgcg cgcgcgatgt
     1801 ccaccggctt ctgcgaggga tccgacggat agagctcgat ctcgagggac ttcgcgagcg
     1861 tggcgagctg ctcgatcgcc gccgggcggt acacgtcggc ggagaccagc agcaccttct
     1921 tcttcgtcgt gttcttgatg aggcgcgcga gcttgccggc gctggtggtc ttgcccgagc
     1981 cctgcaggcc ggccagcagg atcaccgccg gcggctgcgt cgcgaagctc agcgccgcgt
     2041 tcgagccgcc catgaggtcg accagctcgt ggtggacgat tcccaccagc gcctgcccgg
     2101 gggtgaggct ggcggcgact tcctggccga gcgctctttc cttcaccttg gcgatgaagt
     2161 ccttcaccac cggcacgccc gcgtcggcgt cgaggagcgc gaggcgcacc tcgcgcagca
     2221 tttcctgcac gttgtcctcg gtaaggcgcg actgtcc
//
LOCUS       DASVII010000639         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_389359,
            whole genome shotgun sequence.
ACCESSION   DASVII010000639 DASVII010000000
VERSION     DASVII010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389359"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF309_08300"
     CDS             complement(<1..357)
                     /locus_tag="VF309_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466628.1"
                     /translation="MESGVERTKHNAHLAAALRWSTRAEVDWAQMAAASLGMAGPIVA
                     GIVAGHVESGFAASIGGLMLGGVGSCASAREQLREIALAIAPAIAAIVAAAGLAGHGF
                     GSAIVLLLVGAACLLGG"
     gene            376..>1894
                     /locus_tag="VF309_08305"
     CDS             376..>1894
                     /locus_tag="VF309_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7466629.1"
                     /translation="MPLLQLSEISLAYGHVPLLDHADLVIEPGERIGLIGRNGTGKSS
                     LLRIIAGESKPDDGKLFASPGLKLASVPQEPAFAEGLTVFEAVAEGVGELKRLLVDYH
                     AAAHEGAMDRMQHLQEELESRNGWTVEHRIEATLSRLVLSADDKVSALSGGLKKRVAL
                     ARALVMEPQLLLLDEPTNHLDVTAIEWLEETLATLPTAVLFVTHDRRFLDRVAQRIVE
                     LDRGRLQSYPGNFSEYEKRKAEMLATEAVMNRKFDKELAKEEAWIRQGVEARRTRNEG
                     RVRRLEALRIERAARRARVGKVELAVSQGERSGRLVAELEHVSKRYGDKIVVDDFSTR
                     IMRGDKIGLIGANGTGKTTLLKLILGEIEPDSGTVRRGTKVAVAYFDQFRAALDDEAT
                     LSDTIAPGSDFVEIDGGRKHVISYLGDFLFPPERARAPVKSLSGGERNRLLLARLFSR
                     PANVLVLDEPTNDLDIETLELLEALLQEYKGTLFLVSHDRAFLDNVVTQTIAHEGG"
BASE COUNT          323 a          693 c          615 g          263 t
ORIGIN      
        1 cccaccgaga aggcaggcgg cgcctacgag cagcagcacg atcgcgctcc cgaatccgtg
       61 gcccgcgagg cccgcggccg caacaatcgc cgcgatggcg ggcgcgatcg ccagtgcaat
      121 ttcgcgcaac tgctcgcgcg cgctggcgca cgatccgact ccgcccagca tgaggccgcc
      181 gatggaggcc gcgaagccgg actcgacgtg gcccgcgacg attccggcga cgatcggccc
      241 cgccatgccg aggctcgcgg cggccatctg cgcccaatca acctcggcgc gggtggacca
      301 ccgcagcgcc gccgcgaggt gcgcgttatg cttcgtccgt tcaacgccgc tttccatggc
      361 gatgagacta tcaccgtgcc cctgctccaa ctctccgaaa tctccctcgc ctacggtcac
      421 gttccgctgc tggaccacgc ggacctcgtg atcgagcccg gcgagcgcat cgggctcatc
      481 ggccgcaacg gcacggggaa gtcgagcctc ctgcggatca tcgccggaga atcgaagccc
      541 gacgacggca agctcttcgc gtcgcccggc ctcaagctcg cgagcgtgcc ccaggagccc
      601 gcgttcgccg agggcttgac ggtgttcgag gccgtagccg aaggcgtggg cgagctcaag
      661 cgcctgctcg tcgactatca cgccgcggcg cacgaagggg ccatggatcg catgcagcac
      721 ctccaggagg agctcgagtc ccgcaacgga tggaccgtcg agcaccgcat cgaggcgacg
      781 ctgagccggc tcgtgctttc cgcggacgac aaggtctccg cgctctccgg gggattgaag
      841 aagcgcgtcg ccctcgcccg cgcgctggtg atggagccgc agctcctcct cctcgacgag
      901 ccgaccaacc atctcgacgt caccgccatc gagtggctcg aggagacgct cgccacgctt
      961 cccaccgccg tcctcttcgt cacgcacgac cggcgcttcc tcgaccgcgt cgcgcagcgc
     1021 atcgtcgagc tcgaccgcgg gcggctgcag agctaccccg gcaacttcag cgagtacgag
     1081 aagcgcaagg ccgagatgct cgccactgaa gcggtgatga accgcaagtt cgacaaggag
     1141 ctcgccaagg aagaagcgtg gatccgccag ggcgtcgagg cgcggcgcac ccgcaacgag
     1201 ggccgcgtgc gccgtctcga ggcgctgcgc atcgagcgcg cggcgcggcg cgcgcgcgtg
     1261 ggcaaggtcg agctcgcggt ctcccagggc gagcgctccg ggcgcctcgt cgccgagctc
     1321 gagcacgtgt cgaagcgcta cggcgacaag atcgtcgtcg acgatttctc cacgcgcatc
     1381 atgcgcggcg acaagatcgg cctcatcggc gcgaacggaa ccggcaagac cacgctgctg
     1441 aagctgatcc tgggcgagat cgagcccgac tcgggaaccg ttcgccgcgg cacgaaggtc
     1501 gccgtcgcct acttcgacca gttccgcgcg gccctggacg acgaggccac gctatcggac
     1561 accatcgcgc cgggaagcga cttcgtcgag atcgacggcg gaaggaagca cgtcatcagc
     1621 tacctcggcg acttcctctt ccctccggag cgcgcccgcg cgccggtgaa gtcgctgtcg
     1681 ggcggcgagc gcaatcgatt gctcctcgcg cggctcttca gtcgccccgc caacgtgctc
     1741 gtgcttgacg agccaaccaa cgacctcgac atcgagacgc tcgagctcct cgaggcgctg
     1801 ctgcaggagt acaagggcac gcttttcctc gtgagccacg accgcgcctt cctcgacaac
     1861 gtcgtgaccc agaccatcgc ccacgaaggc ggcg
//
LOCUS       DASVII010000640         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_496944,
            whole genome shotgun sequence.
ACCESSION   DASVII010000640 DASVII010000000
VERSION     DASVII010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496944"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF309_08310"
     CDS             complement(<1..212)
                     /locus_tag="VF309_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466630.1"
                     /translation="MICASCGVFAGFYAEAESLAPHHTPEGFRNNYPHDAKQSFWLWK
                     WEQMKEGVPQPPPGGWKIPFVKTDAAA"
     gene            351..>1541
                     /locus_tag="VF309_08315"
     CDS             351..>1541
                     /locus_tag="VF309_08315"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7466631.1"
                     /translation="MAGPLANVKVVEMGTLIAGPYCARLLAEFGAEVIKIEAPGEGDP
                     LRKWRKLHEGNSLWWYAQARNKKSVAVNLRDPQGQAIVRGLAKGADIVIENFRPGTLE
                     KWGLGYDELAKDNPGLIMVRISGFGQTGPYKDRPGFGAIGESMGGMRYVTGYPDRAPV
                     RVGISIGDSLAAMFGVIGALSALHHRAASGKGQVVDVALYEAVFAMMESMLPEFGIGG
                     FVRERSGASLPGIVPSNTYPCKDGNYVVIGANADSIFKRMMRAIGRPDLADDPSLATN
                     DGRVKRTEELDAAIAAWTARHDLEEVLAVLDKAEVPSGRIYSVADIVADMHYQARGMI
                     ERHKLGDRDLLLPGIVPSNTYLCGDGKYVVIGANGDSIFKRMMAAIDRADLAEDPGLA
                     DNAGR"
BASE COUNT          268 a          498 c          532 g          243 t
ORIGIN      
        1 gccgccgcgt cggtcttcac gaacggaatc ttccagcctc ccggcggcgg ctgcgggacg
       61 ccctccttca tctgctccca cttccagagc cagaagctct gcttcgcatc gtgcggatag
      121 ttgttgcgga agccctccgg cgtatggtgc ggcgcgaggg actcggcctc ggcgtagaag
      181 ccggcgaaca ctccgcagga ggcgcagatc ataatggcag ccagtctacc gcgagcccgt
      241 ctgattcggc ccatggggcg cccgatcgat gtggggtgga cggttcgcat catgccgcag
      301 gatgcgtggg cccgtcgccg ggtgcaaaat aagcgcgagg aggaatcgat ttggccggac
      361 cgctggcgaa cgtcaaggtc gtcgaaatgg gaacgctgat cgcgggcccc tattgcgcgc
      421 gcctcctcgc ggagttcggc gcggaggtga tcaagatcga ggcgcccggc gagggcgatc
      481 cgctgcgcaa gtggcgcaag ctccacgagg gcaactcgct gtggtggtac gcgcaggcgc
      541 gcaacaagaa atccgtcgcg gtgaaccttc gcgatccgca gggccaggcg atcgtgcgcg
      601 ggctcgcgaa gggcgccgac atcgtgatcg agaacttccg ccccggcacc ctggagaaat
      661 ggggcctcgg ctacgacgag ctcgcgaagg acaatccggg gctcatcatg gttcgcatct
      721 cgggattcgg ccagacgggc ccgtacaagg accggccggg cttcggcgcg atcggcgagt
      781 cgatgggcgg catgcgctac gtgacgggct acccggaccg cgcaccggtg cgcgtgggaa
      841 tctcgatcgg ggattcgctc gcggcgatgt tcggcgtgat cggagcgctg agcgcgttgc
      901 accaccgcgc cgcatccggc aaggggcagg tggtcgacgt cgcgctctac gaggcggtgt
      961 tcgcgatgat ggagtcgatg cttcccgagt tcggcatcgg cggcttcgtg cgcgaaagga
     1021 gcggcgcgtc gctgccgggc atcgttccct cgaacactta tccgtgcaag gacggaaact
     1081 acgtggtgat cggcgccaac gccgactcca tattcaagcg aatgatgcgc gccatcggca
     1141 ggcccgacct cgccgacgat ccctccctcg cgaccaacga cggacgggtc aagcgcaccg
     1201 aggagctcga cgcggcgatc gccgcgtgga ccgcgcgcca cgacctcgag gaggtcctcg
     1261 cggtgctcga caaggcggaa gtcccgtcgg gacggatcta ctcggtagcc gacatcgtcg
     1321 ccgacatgca ctaccaggcg cgcggaatga tcgagcgcca caagctcggc gatcgcgacc
     1381 ttctccttcc cggcatcgtg ccttccaaca cctatctttg cggcgacggc aagtacgtcg
     1441 tgatcggcgc caacggcgac tcgatcttca agcgcatgat ggcggcgatc gatcgagccg
     1501 atctcgcaga ggatccgggg ctcgccgaca acgccgggcg c
//
LOCUS       DASVII010000641         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_569260,
            whole genome shotgun sequence.
ACCESSION   DASVII010000641 DASVII010000000
VERSION     DASVII010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569260"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..>1565
                     /gene="mrdA"
                     /locus_tag="VF309_08320"
     CDS             108..>1565
                     /gene="mrdA"
                     /locus_tag="VF309_08320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7466632.1"
                     /translation="MMLGQALRDQQHELMVFRSRLMLSGLVIIVAFAILLGRFSWLQV
                     VQREYYHTLAEANRISVVPLVPNRGLILDRNGEVLAANYSAYTLEITPSKVANVERAI
                     DELSSLIDVAPRDRRRFKKLQEESKNFESLPIRTRLTEEEVARFAVNRFRFPGFEIKA
                     RLFRSYPQGEVASHAIGYIGRINDADVKRIDEQGLTPNYKGTDHIGKLGIEGAYEKEL
                     HGVTGSEQVEIDAGGRAIRALSRSEPLSGNNLVVTLDLKLQKIAEDAFQDYKGALVAI
                     DPKTGEVLALVSKPGFDPNLFVDGIDPQNWDALNNSPDKPLNNRALRGQYPPGSTIKP
                     FMALAALEYRKRSTEFTISDPGYFALPGVAHRYRDWKKEGHGLVNMHKSIVISCDTYY
                     YGLANELGIDAIHSFLTRFGFGARTGIDIDGELAGLAPSQEWKWQRFKQKWYAGDTVS
                     VGIGQGYLLTTPLQLAAATATIANGGVPVLPHLLRS"
BASE COUNT          281 a          543 c          496 g          245 t
ORIGIN      
        1 ggtgtggcct acttcatctc gcccgtcgtg acggcgttcc tgtgggggcc ggtgagcggg
       61 ctcctctaca tgcagtccgt gcggcgccgg cgcggggagt ccggatcatg atgctcggcc
      121 aggcgctgcg cgaccagcag cacgagctca tggtctttcg cagccgcctc atgctctcgg
      181 ggctcgtcat catcgtcgcc ttcgcgatcc tgctcgggcg cttcagctgg ttgcaagtcg
      241 ttcaacgcga gtactaccac acgctcgccg aggccaatcg catctccgtc gtgccgttgg
      301 tcccgaaccg cggcctcatc ctcgacagga acggcgaggt gctggccgcc aactactcgg
      361 cctacacgct cgagatcacg cccagcaagg tcgcgaacgt cgagcgcgcc atcgacgagc
      421 tttcgtcgct gatcgacgtc gcgccgcgcg accggcggcg cttcaagaag ctccaggagg
      481 agagcaagaa cttcgaaagc ctgccgatcc gcacgcgctt gaccgaagag gaggtggcgc
      541 gcttcgcggt caatcgattc cgtttccccg gcttcgagat caaggcgcgc ctcttccgct
      601 cctatccgca gggcgaagtg gcctcgcatg cgatcggcta catcggccgc atcaacgacg
      661 ccgacgtgaa gcgcatcgac gagcagggcc tcacgcccaa ctacaagggc accgaccaca
      721 tcggcaagct cggcatcgag ggcgcctacg agaaggagct gcacggcgtt accggctcgg
      781 agcaggtcga gatcgatgcc ggcggtcgcg ccatacgcgc gttgtcgcga agcgagccgc
      841 tttccggcaa caacctggtg gtgacgctcg acctcaagct gcagaagatc gccgaggacg
      901 ccttccagga ctacaagggc gcgctggtgg cgatcgaccc gaagacgggc gaagtgctgg
      961 cgctcgtctc caagccggga ttcgatccga acctcttcgt ggacggcatc gacccccaga
     1021 actgggacgc gctcaacaac tcgccggaca agccgctcaa caaccgcgcg ctgcgcggcc
     1081 aatacccgcc gggctcgacc atcaagccct tcatggcgct cgcggcgctc gaataccgga
     1141 agcgctcgac ggaattcacc atctcggacc cgggctattt cgcgctgccg ggcgtcgcgc
     1201 accgctaccg cgactggaag aaggagggcc acgggctcgt caacatgcac aagtcgatcg
     1261 tgatctcgtg cgacacgtac tactacgggc tcgccaacga gctcggcatc gacgcgatcc
     1321 acagcttcct cacgcgcttc ggcttcggcg cgcgcaccgg catcgacatc gacggcgagc
     1381 tcgcgggcct cgcgccgtcc caggagtgga agtggcagcg cttcaagcag aagtggtacg
     1441 cgggcgacac cgtgagcgtg gggatcggcc agggctacct gctcaccacc ccgctgcagc
     1501 tcgccgcggc gacggcgacc atcgccaacg gaggagtgcc agtgctcccg cacctgctga
     1561 ggtcg
//
LOCUS       DASVII010000642         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_569279,
            whole genome shotgun sequence.
ACCESSION   DASVII010000642 DASVII010000000
VERSION     DASVII010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569279"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VF309_08325"
     CDS             <1..759
                     /locus_tag="VF309_08325"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7466633.1"
                     /translation="LDGSVLHIRFNNPAKHNALSVDMWEAVPPLLDRAEKDDNVRMVV
                     LSGEGGKAFISGADISQFEDMRAAKEAVKRYEAMAEAALAGILEFEKPTVACISGYCI
                     GGGVNVAISCDIRVASSNSVFSVPASRLGLGYRYSAMKNLTQLVGPGFAKDIFFTGRR
                     LDAAEALRIGLINRVAEPEGLDALLAEYTTSIGTGAPLTIKAGKRIIRDVLKRDDADM
                     DLARKMILDCFESEDYAEGRKAFMEKRKPVFKGK"
     gene            799..>1633
                     /locus_tag="VF309_08330"
     CDS             799..>1633
                     /locus_tag="VF309_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7466634.1"
                     /translation="MLIALRTLAASLALVASSAFAAWPEKPIKVYIGYAPGGSTDVVG
                     RLISQKLGEKLGQPIIIENKPGGAGDFAAEMMLQAPPDGYTLFMTTVAVHAINPGLYK
                     TQKFHPLNDFTPIALICSYPMIMIASPTTTFKTPAELKELAAKTPTFYSSSGVGSPGH
                     LAGELLVKSLGANIIHVPYKGGSPSVLAVMTGEAQVNFATLPAVVPQIRGGKVRAIGL
                     ASNSRNPAVPDVPTMAELGIADFDVGTWTGFVGPRGVPADVVRKINAAVTAVVNDPVV
                     RQ"
BASE COUNT          295 a          542 c          535 g          261 t
ORIGIN      
        1 ctcgacggct ccgtgctcca catccgcttc aacaatccgg cgaagcacaa cgcgctctcg
       61 gtcgacatgt gggaagcggt gccgccgctc ctcgatcgcg cggagaagga cgacaacgtg
      121 cgcatggtgg tgctctcggg agagggcggc aaggccttca tctccggcgc cgacatctcg
      181 caattcgagg acatgcgcgc cgcgaaggaa gcggtgaagc gctacgaggc gatggccgag
      241 gccgcgctcg ccggcatcct cgaattcgag aagccgacgg tcgcctgcat cagcggttat
      301 tgcatcggcg gcggcgtgaa cgtggcgatc agctgcgaca tccgcgtggc ctcctcgaac
      361 tcggtcttct ccgtgcccgc ctcgcggctg ggactcggct atcgctattc cgcgatgaag
      421 aacctcacgc agctcgtagg cccgggattc gccaaggaca tcttcttcac cgggcgccgg
      481 ctcgacgcgg ccgaggcgct gcgcatcggt ctcatcaacc gcgtggccga gcccgaaggt
      541 ctggacgcgc tgctcgccga gtacacgact tcgatcggca ccggcgcgcc gctcaccatc
      601 aaggccggca agcggatcat ccgcgacgtg ctgaagcgcg atgacgccga catggatctc
      661 gcgcggaaga tgatcctgga ttgcttcgag agcgaggatt acgcggaggg acgcaaggcg
      721 ttcatggaga agcgtaagcc ggtgttcaag ggcaaatgaa gtcattcccg tgaagacggg
      781 aatccaggct cggagacaat gttgatcgca cttcgcacgc tcgccgcatc cctggcactc
      841 gtcgcatcat ccgcattcgc ggcgtggccc gaaaaaccaa tcaaggtcta catcggctac
      901 gcgccgggcg gatccaccga cgtcgtcggg cgcctcatct cgcagaagct gggcgagaag
      961 ctgggacaac cgatcatcat cgagaacaag cccggcggcg cgggcgactt cgccgcggag
     1021 atgatgctgc aggccccgcc cgacggctac acgctcttca tgacgacggt ggcggtgcac
     1081 gccatcaacc ccggtctcta caagacgcag aaattccacc cgctgaacga cttcacgccg
     1141 atcgcgctca tctgctcgta cccgatgatc atgatcgcgt cgccgacgac caccttcaag
     1201 acgccggcgg aattgaagga gctcgccgcg aagacgccga ccttctactc gtcgtcgggc
     1261 gtgggctcgc cgggtcacct cgcgggcgag ctgctggtga agagcctggg agcgaacatc
     1321 attcacgtgc cctacaaggg cggctcgccg tcggtgctcg cggtcatgac cggcgaggcg
     1381 caggtgaatt tcgcgacgct gcccgcggtg gtgccgcaga tccgcggcgg caaggtgcgc
     1441 gccatcggcc tcgcgtcgaa ctcgcgcaat cccgcggtgc ccgacgtgcc caccatggcg
     1501 gagctcggca tcgccgactt cgacgtcggc acgtggaccg gcttcgtcgg cccgcggggc
     1561 gtgcccgccg acgtggtgcg gaagatcaat gcggcggtca cggcggtggt gaacgatccc
     1621 gtggtccgtc agc
//
LOCUS       DASVII010000643         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_713066,
            whole genome shotgun sequence.
ACCESSION   DASVII010000643 DASVII010000000
VERSION     DASVII010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713066"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VF309_08335"
     CDS             <1..896
                     /locus_tag="VF309_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7466635.1"
                     /translation="CGTCKVELVEGQIASLRPDAPGLGERDRAKNRVLACQARPGSDC
                     TIKVRLREENVPRHRPAKFTAKLTGVRDLTHDMREFTFSAKDAPGFLPGQYALFYLPG
                     IEAPRTYSMSNVDDGSGAWQFIVRRVAGGVGTVALFDKVAIGSEVTLDGPYGLAYLRP
                     DSPRDIVLIGGGSGLAPMLSLARGAVAEPRLDGRRIHVFYGARTARDLCGAAELEALP
                     GYASRITFTPVLSHHGDDSVETWDGAKGFVHDIARDFVGERWNDYEYYFAGPPPMAEA
                     VQRMLIDKRVPFPQVHFDSFY"
     gene            936..>1760
                     /locus_tag="VF309_08340"
     CDS             936..>1760
                     /locus_tag="VF309_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466636.1"
                     /translation="MSKLKLSLGCWNYDRTRGLLEGRIQPDGIDLTYLNMPVEETFFR
                     MLRHREFDVAEMSLSSYTVSLFKEQKRFIAIPVFPSRFFRHSCIYVNAASGIKEPKDL
                     IGRKVGNPEYQMTAPVWIRGILQDEYGVPVDSVTYYTGGEEEPGRSEKLKLDLPPNIK
                     VQPIGPTDTLAAMLRDGGIDAFYTARMPSTYHTGGGKVKRLFENYMEVEQAYYRKTRI
                     FPIMHTVAIRKSLYEKHPWVAMSLYKAFVQSQRETYEDLYVTAALKAMLPWLTKHVE"
BASE COUNT          304 a          599 c          568 g          289 t
ORIGIN      
        1 cgtgcggcac gtgcaaggtg gagctcgtcg aaggccagat cgcttccctg cgtcccgacg
       61 cgcccggatt gggcgagcga gaccgcgcga agaaccgcgt gctcgcctgc caggcgcggc
      121 ccggctccga ttgcaccatc aaggtccgcc tgcgcgagga gaatgtcccg cgccaccggc
      181 ccgcgaagtt cacggcgaag ttgaccggcg tgcgcgacct cacgcacgac atgcgcgagt
      241 tcaccttctc cgcgaaggac gcgcccggct tccttcccgg ccagtacgcg ctcttctatc
      301 ttcccgggat cgaggcgccg cgcacctact cgatgtcgaa tgtcgacgac ggcagcggcg
      361 cgtggcagtt catcgtgcgc cgcgtcgccg gcggggtcgg caccgtggcg ctcttcgaca
      421 aggtcgcgat cggctccgag gtcacgctcg acgggccgta tggcctcgcg tacctgcgcc
      481 ccgacagccc gcgcgacatc gtcctcatcg gcggcggttc gggcctggcg cccatgctct
      541 cgctcgcgcg gggcgcggtc gcggagccgc gcctggacgg tcgcaggatc catgtgttct
      601 acggcgcgcg caccgcgcgc gacctgtgcg gcgcggccga gctcgaggcg cttcccggct
      661 acgcaagccg catcaccttc acgcccgtcc tctcgcacca tggcgacgac tccgtcgaga
      721 cgtgggacgg ggcgaaaggc ttcgtgcacg acatcgcgcg cgatttcgtg ggcgagcggt
      781 ggaacgacta cgagtactac ttcgcggggc ctccgccgat ggccgaggcg gtgcagcgga
      841 tgctgatcga caagcgcgtg ccttttccgc aggtgcactt cgatagcttt tactgacgtt
      901 gtcagggctg attcaaacgc tgtaaggaaa acgcagtgtc aaagctgaaa ctctctctgg
      961 gctgctggaa ctacgaccgc acgcgcggcc tgctggaagg gcgcatccag cccgacggca
     1021 tcgacctcac gtacctgaac atgccggtgg aggagacctt cttccggatg ctgcgccacc
     1081 gcgagttcga cgtggcggag atgtcgctgt cgtcgtacac ggtgtcgctc ttcaaggagc
     1141 agaagcgctt catcgcgatt ccggtgttcc cgtcacgctt cttccgccat tcctgcatct
     1201 atgtgaacgc cgcctccggc atcaaggagc cgaaggacct gatcggcagg aaggtgggca
     1261 accccgaata ccagatgacg gcgccggtgt ggatccgcgg catcctgcag gacgagtacg
     1321 gcgttcccgt cgacagcgtc acctactaca ccggcggcga ggaggagccg ggacgcagcg
     1381 agaagctgaa gctcgatctc ccgcccaaca tcaaggtcca gccgatcggg cccaccgaca
     1441 cgctggccgc gatgctgcgc gacggcggca tcgacgcctt ctacaccgcg cgcatgccct
     1501 ccacctatca cacgggcggg ggcaaggtga aacgcctctt cgagaactac atggaggtcg
     1561 agcaggcgta ctaccgcaag acgaggatct tccccatcat gcacaccgtc gcgatccgca
     1621 agagcctcta cgagaagcac ccctgggtcg cgatgtcgct ctacaaggcc ttcgtgcagt
     1681 cgcagcgcga gacgtacgag gatctctacg tcaccgcggc gttgaaggcg atgctgccgt
     1741 ggctcaccaa gcacgtggag
//
LOCUS       DASVII010000644         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_65969, whole
            genome shotgun sequence.
ACCESSION   DASVII010000644 DASVII010000000
VERSION     DASVII010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65969"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VF309_08345"
     CDS             <1..563
                     /locus_tag="VF309_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466637.1"
                     /translation="EEVPKVTEDVFEDLLADERAESEADDEDPMQPMNAAGALRAAER
                     GSPAHQVSVGSSYLSGIDWDGNPIPVDLERAKHWLEEAARTGLPSALYLLGTMFEEGL
                     GMTPNIERAMRLYEKGADRRAFLPSIQCCIRLARIHAQAGTERYSPDEAKRRYQAVLE
                     IAAKYGIEAEATAQMEEAMIYLGLAR"
     gene            575..1396
                     /locus_tag="VF309_08350"
     CDS             575..1396
                     /locus_tag="VF309_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7466638.1"
                     /translation="MTSRSRLLALGAIVLWGGLATLALELAALPPFLLTGIALVIGSL
                     VGWRALRARPPPPAALALGVYGLFGFHFFLFLALRNAPPVEANLVNYLWPLLIVVLAP
                     ALVPGIRPSLRHAAAAIMGFVGAALLITAGAEVSGRAAPVGYLYALVSAFIWSTYSLM
                     TRRFAHFPSAYVAWFCLVSGAAALACHFVFEPAYRPSATQWPFIVAMGLGPMGLAFQL
                     WDRAMKGGDPRVIGNLAYLTPLLSTLMLAAFAGQHLTATSLVAMAIILGAAWIGS"
     gene            complement(1393..1950)
                     /locus_tag="VF309_08355"
     CDS             complement(1393..1950)
                     /locus_tag="VF309_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX7466639.1"
                     /translation="MTLEAYAKDRKDFRVQVIAHKKNRTVHLGEHVTLLFEDELTMRY
                     QVQEMLRAEKIFEERGIQDEIDAYNPLIPDGRNFKATMMIEYEEERERRAALAKMKGV
                     EGETWIRVEGLPRVYAIADEDLERENDEKTSSGHFLRFELTKEMADAMKYGVGLAMGV
                     DHPAYTATLDPVPAATRAALVKDLA"
BASE COUNT          267 a          656 c          676 g          364 t
ORIGIN      
        1 cggaggaagt gccgaaggtg accgaagacg tcttcgagga tttgctcgcc gatgagcgcg
       61 ccgagtcgga ggcggacgac gaggacccga tgcagccgat gaacgccgcc ggggcgctga
      121 gggccgccga gcggggaagc ccggcccacc aagtcagcgt ggggagctcg tacctttcgg
      181 gaatcgactg ggacggcaac ccgatccccg tggacctcga gcgcgcgaag cattggctcg
      241 aggaggcggc gcgcacgggt ttgccatcgg cgctctatct gttgggcacg atgttcgagg
      301 aaggcctggg catgacgccg aacatcgagc gcgcgatgcg tctctacgag aaaggagccg
      361 accggcgcgc gttcctgccg tccatccagt gctgcattcg cctcgcccgc atccatgcgc
      421 aggccggcac ggaacgctat tcgcccgacg aggcgaagcg ccggtaccag gcggtgctgg
      481 agatcgcggc gaagtacggg atcgaggccg aggcaacggc gcagatggaa gaggcgatga
      541 tctacctggg actcgcacgc tgactggaaa tccggtgact tcccggtcga ggctgctggc
      601 gctcggcgcg atcgtcctgt ggggcgggct cgcgacgctc gcgctcgagc tcgccgcgct
      661 gccgccattc ctgttgacgg ggatcgcgct cgtcattgga tcgctggtgg gatggcgcgc
      721 gttgcgagcg cgcccgccgc cccccgcggc gctcgcgctc ggggtctacg ggctcttcgg
      781 cttccacttc ttcctcttcc tcgcgttgcg caacgcgccg ccggtggagg cgaacctcgt
      841 caactatctg tggccgctcc tcatcgtggt gctcgcgccg gcgctcgtgc cgggaatccg
      901 tcccagcctt cggcacgcgg ccgcggcgat catgggcttc gtgggcgccg cgttgctgat
      961 caccgcgggg gccgaggttt cggggcgggc ggcgcccgtc ggctacctct atgcgctggt
     1021 ctccgcattc atctggtcga cgtactcgct gatgacgcgg cgcttcgcgc atttcccgag
     1081 cgcctacgtc gcttggttct gcctcgtctc gggcgcggcg gcgctcgcgt gccatttcgt
     1141 gttcgagccc gcctaccggc ccagcgcgac gcagtggcca ttcatcgtcg cgatgggcct
     1201 gggaccgatg gggctcgcgt tccagctctg ggatcgcgcg atgaagggag gcgacccccg
     1261 cgtgatcggc aatctcgcct atctcacgcc gctcctgtcc acgctgatgc tcgcggcctt
     1321 cgccggccag catctcacgg cgacttcgct cgtcgccatg gcgatcatcc tcggcgccgc
     1381 ctggatcggc tcctaggcga ggtccttcac caaagcggcc cgggtcgcgg ccggcaccgg
     1441 atcgagggtc gccgtgtagg cggggtgatc gacgcccatc gcgaggccga cgccgtactt
     1501 catcgcgtcg gccatttcct tcgtgagctc gaagcgcagg aagtgaccgg aggaagtctt
     1561 ctcgtcgttc tcgcgctcga ggtcctcgtc ggcgatcgca tagacgcgcg gcaggccttc
     1621 gacgcggatc cacgtctcgc cctcgactcc cttcatcttc gcgagggcgg cgcggcgctc
     1681 gcgctcctcc tcgtactcga tcatcatcgt cgccttgaag ttgcgcccgt cggggatgag
     1741 cgggttgtag gcgtcgatct cgtcctggat gccgcgctcc tcgaagatct tctcggcgcg
     1801 cagcatctcc tgcacctggt agcgcatggt gagctcgtcc tcgaagagaa gcgtcacgtg
     1861 ctcgccgagg tgaacggtgc ggttcttctt gtgcgcgatc acctgtacgc ggaagtcctt
     1921 gcggtccttc gcgtaggcct cgagggtcat cagggattga gga
//
LOCUS       DASVII010000645         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_30427, whole
            genome shotgun sequence.
ACCESSION   DASVII010000645 DASVII010000000
VERSION     DASVII010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30427"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VF309_08360"
     CDS             <1..1200
                     /locus_tag="VF309_08360"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX7466640.1"
                     /translation="VCATAGHHMESLLAAYGSGAAKASHDLGRPVLNVDIGGGTTKLA
                     LVEGGRVVHTAAIHVGGRLAVVEPSPRGEGALVRLDPAGKQLAANAGVDWSIGGSATG
                     IQMDRVAGWMADALVAALTQSPTPHPVDALWLTDPLEPVEGIAGVMFSGGVAEYVYGR
                     EERDFGDLGKRVGLALRKRLDAGALPYPLLPAGECIRATAIGASEYSVQLSGNTVYIS
                     NPAALLPRKNLQVLQPDLSLAGEIDAARVTQAIRRHFEAFDLVEGESEVALAFRWQGP
                     PAYRRLAALARGIVEALPMTLLEAKPVFLVLDGDIAHSLGALLKDEWAVASEVLVIDG
                     VSLWDFDYVDLGRVRMPSYTVPVTIKSLVFSEDPRGPKQRGHGHDHGHDHEHGHGHHH
                     HAPGGGA"
     gene            1197..1871
                     /locus_tag="VF309_08365"
     CDS             1197..1871
                     /locus_tag="VF309_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7466641.1"
                     /translation="MSELARTLADALRMLEAAEVIDFNGHMSCRVPGTGHILINSGKS
                     VRSRITPADIIAIDMDGKPVEGDVVPPMEFHIHTEIYRRRPDVNAVAHTHPLWSTLFS
                     MVGETVQPVTMQAAVMGPVQAFSKTASINRQDLAVELAAALGSHQVVMLKSHGAVVVG
                     ADVVEAFVLAIYLEETAKRQYLARALGTPYALVAAEVEKIGANLRKPHLLRKVWDFHH
                     AKLGAP"
     gene            1868..>3049
                     /locus_tag="VF309_08370"
     CDS             1868..>3049
                     /locus_tag="VF309_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7466642.1"
                     /translation="MSLAAAAPHPADARRFEWGWVVIGLCVAITVYLAIVPLGFLVWQ
                     SFFTPQTADEAARFTLSNYTEAYGSSETWRLFRNSVLFAIGTSSFSFLVGTGLAWMNE
                     RTNTPFKTLFYALSIIPLIIPSILFTVAWILLASPKIGILNLVLKNALGIDDPLFDVY
                     SLWGMIWVDGLHYSPMAFLLMSAAFRSMDPALEESAMMSGANVLQVAWRVTLRLSWPA
                     IFATILILFVRAIESFEVPALLGLPVGIQVFTSSIYQAVHRYPSQVGLASAYAVTLLA
                     ITTVGVYFQSRLSSRGSRYATMTGKGFRPRQIDLGAWRWFTAALFIAYFLLIVVLPFS
                     VLLWSSFQKFYAVPSWHALQNLTLDPYRFILTYPNLGSSVWNSLLLAFGSATLIMLVT
                     SV"
BASE COUNT          449 a         1068 c          996 g          536 t
ORIGIN      
        1 gtgtgcgcca ccgccggcca ccacatggag tcgctgctgg ccgcctacgg ctcgggcgcg
       61 gcgaaggcct cccatgacct cggccgtccc gtgctgaacg tggatatcgg cggcgggacc
      121 accaagctcg cgctggtcga aggcgggcgt gtggtgcata ccgccgcgat tcatgtcggc
      181 gggagacttg ccgttgtgga accctctccc aggggagagg gagcccttgt gcggctcgat
      241 cccgccggca agcaactggc agccaacgcc ggagtcgact ggagcatcgg cggttcggcg
      301 accgggatcc agatggaccg cgtcgccgga tggatggccg acgcgctcgt cgccgcgctc
      361 acgcaaagcc ccacgccgca tccggtcgat gcactgtggc tcaccgatcc cctcgagccg
      421 gtcgaaggca tcgcgggcgt gatgttctcg ggcggcgtcg cggaatacgt ctatgggcgc
      481 gaggagcgcg acttcggcga tctcggcaag cgcgtgggcc tcgcgctgcg caagcgcctc
      541 gacgcaggcg cgcttccgta tccgttgctg cccgcgggcg agtgcatccg cgccaccgcc
      601 attggcgcct ccgagtacag cgtgcagctc tccggcaaca cggtctacat ctcgaatccc
      661 gcggcgctcc tgccgcgcaa gaacctgcag gtgctccagc ccgacctctc gctcgcgggc
      721 gagatcgacg cggcgcgcgt gacacaggcc attcgccgcc acttcgaggc cttcgacctg
      781 gtggaaggcg agagcgaggt ggccctcgcg ttccgctggc agggcccgcc cgcgtaccgg
      841 cggctcgcgg cgctcgcgcg cggcatcgtc gaggccctgc cgatgacgct tctcgaggcg
      901 aagccggtct tcctggttct cgacggcgac atcgcgcact cgctgggcgc gcttctcaag
      961 gacgaatggg cagtggcctc cgaggtcctg gtgatcgacg gcgtctctct ctgggacttc
     1021 gactacgtgg acctcggccg cgtgcgaatg ccctcgtaca cggtgccggt caccatcaag
     1081 tcgctcgtct tcagcgagga tccgcgcggg ccgaagcagc gcggacacgg ccacgaccat
     1141 ggccacgacc acgagcatgg ccacggccac catcatcacg cgccgggggg cggggcatga
     1201 gcgagctcgc caggaccctc gccgacgccc ttcgcatgct cgaggccgcg gaagtgatcg
     1261 atttcaacgg gcacatgagc tgccgcgtgc cgggaaccgg gcacatcctc atcaattcag
     1321 gcaagtcggt gcgcagccgg atcacgcccg cggacatcat cgccatcgac atggacggca
     1381 agcccgtcga aggcgatgtc gtgccgccga tggagttcca catccacacc gagatctacc
     1441 gccgccggcc cgacgtgaac gcggtcgccc atacccatcc cctgtggtcg acgctcttca
     1501 gcatggtggg cgagacggtc cagccggtga cgatgcaagc cgccgtgatg ggcccggtgc
     1561 aggccttctc gaagaccgct tcgatcaacc ggcaggatct cgccgtcgag ctcgccgccg
     1621 ctctcggcag ccatcaggtc gtgatgctca agtcccacgg cgcggtggtg gtgggcgcgg
     1681 acgtggtcga agcgttcgtc ctcgcgatct acctcgagga gacggcgaag cgccagtatc
     1741 tcgcgcgcgc cctcgggacg ccctacgcgc tcgtcgcggc cgaagtcgag aagatcggcg
     1801 cgaaccttcg caagccgcac ctccttcgca aggtgtggga cttccaccac gccaagctgg
     1861 gggcgccatg agcctcgccg ccgccgctcc ccatcccgcg gacgcgcgcc gcttcgagtg
     1921 gggctgggtg gtgatcgggc tgtgcgtggc gatcacggtg tatctcgcga tcgtgccgct
     1981 cggcttcctc gtctggcaga gcttcttcac gccgcagacg gccgacgagg ccgcgcgctt
     2041 cacgctttcc aactacaccg aggcctacgg cagcagcgag acctggaggc tcttcaggaa
     2101 ttcggtcctc ttcgcgatcg gcacgtcgag cttctccttc ctcgtgggca cgggcctcgc
     2161 ctggatgaac gagcgcacca acacgccctt caagacactc ttctacgcgc tgtcgatcat
     2221 cccgctgatc attccgagca tcctcttcac ggtggcgtgg atccttctcg ccagcccgaa
     2281 gatcggcatc ctgaacctgg tgctcaagaa cgccctcggc atcgacgatc cgctgttcga
     2341 cgtctactcg ctgtggggaa tgatctgggt ggacgggctg cactattcgc cgatggcgtt
     2401 cctgctcatg tcggcggcgt tccgctcgat ggatccggcg ctcgaggagt ccgcgatgat
     2461 gagcggcgcg aacgtgctgc aggtcgcgtg gcgcgtgacg ctgaggctct cgtggccggc
     2521 gatcttcgcg acgatcctca tcctcttcgt gcgcgcgatc gagtcgttcg aggtgccggc
     2581 gctcctcggg ctgcccgtcg gcatccaggt gttcacgtcg tcgatctacc aggcggtgca
     2641 ccgctatccg agccaggtcg ggctcgcgtc ggcctacgcg gtgacgctcc tcgccatcac
     2701 gacggtgggc gtctatttcc agtcgcggct gtcgagccgc ggcagccgct acgccaccat
     2761 gacgggcaag ggattccgcc cgcgccagat cgacctcggg gcgtggcgct ggttcaccgc
     2821 ggcgctcttc atcgcctact tcctgctgat cgtcgtgctg ccgttctcgg tgctcctgtg
     2881 gtcgtcgttc cagaagttct acgcggtgcc gtcgtggcac gcgctgcaga acctcaccct
     2941 cgacccgtac cggttcatcc tcacgtaccc gaaccttggg agctcggtgt ggaatagcct
     3001 cctcctcgcc ttcgggagcg cgacgctcat catgctggtg acttcggtc
//
LOCUS       DASVII010000646         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_209318,
            whole genome shotgun sequence.
ACCESSION   DASVII010000646 DASVII010000000
VERSION     DASVII010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209318"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1309)
                     /gene="coaBC"
                     /locus_tag="VF309_08375"
     CDS             complement(89..1309)
                     /gene="coaBC"
                     /locus_tag="VF309_08375"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7466643.1"
                     /translation="MASVALAISGSIAAYKAVEVARRLIAAGVAVTPVMTSSAERFVG
                     PLTLSGICGTPVVRDMFATSYPGEVHVDLGRKVDLVLIAPATADTLARLAQGRADDIV
                     AALALSAACPVLVAPAMHTRMWRHPATQRNVATLLADGRIKLIGPVEGALASGESGMG
                     RLADPADIVAVALREMRAARDLAGLRIVVSAGPTVEDLDPVRFLSNRSSGRMGFAVAE
                     RAARRGAQVTLVAGPVELATPALVTRVDVRSTLDMQAALAAAMGPDLASADVLVMAAA
                     VADYAPRERHATKIKKAGETLSIDLAKNPDLLADIGNARTGARPLLVGFAVETAEPDA
                     LVAYARQKLVQKKIDLVVANHAAEAFGGDTNRATFVTDSGADPLPPMTKMDLADRILD
                     RVRALSIARGFVTC"
     gene            complement(1319..>1883)
                     /locus_tag="VF309_08380"
     CDS             complement(1319..>1883)
                     /locus_tag="VF309_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEX7466644.1"
                     /translation="EVVPFAELERQDPKLPRGARVLSQRGIPGFHTHRYRIVRDGAFA
                     VRERWNDSYPPTNQIVRVGTAEGGDADAVTDDPHPEYVADDYLVLLQGPDVKGAAGTT
                     LDPGSRRGGGGMVEVREPGKTGEYGWTEKAGFSHYVAKRRAKDSDATCTGDCPAGDDD
                     ETSRRSPRQGEPARRRQGSRAAGRHLR"
BASE COUNT          294 a          628 c          669 g          292 t
ORIGIN      
        1 gcgatcaaga cgaggggcgt tcccgccgca tcggggcgca tggagacgct catcgaggcg
       61 gcaacgagcg ccgcaaccac cagcgcaatc agcacgtcac gaatcctcgc gcgatggaga
      121 gagcgcgcac gcgatcgaga atccggtcgg cgagatccat cttggtcatc ggggggagag
      181 ggtcggcacc gctgtcggtg acgaacgtag cgcggttcgt gtcgccgccg aaagcctctg
      241 cagcatggtt ggcgaccacg agatcgatct ttttctggac gagcttttgc cgggcgtatg
      301 ccaccagcgc atcgggctcg gcggtctcga cggcgaaccc cacgagcagc ggtcgtgcgc
      361 cggtgcgagc gttcccgatg tcggccagaa ggtcggggtt cttggcgaga tcgatggata
      421 gcgtctcgcc cgccttcttg atcttggtcg cgtggcgctc gcgcggggca tagtcggcaa
      481 cggcagccgc catgaccagg acatcggccg acgcgaggtc gggacccatg gcggccgcga
      541 gagccgcttg catgtccagt gtgcttcgca cgtccacacg cgtgacgagg gccggggttg
      601 cgagctcgac cgggccggcg acgagcgtca cttgggcgcc tcggcgcgcc gcgcgctcgg
      661 caaccgcgaa tcccatgcgg ccgctggaac ggttgctcag gaagcgaacg ggatcgagat
      721 cttccaccgt gggcccggca ctgaccacga tgcgcaggcc ggcgagatcg cgggcagccc
      781 gcatctcgcg cagcgcgacg gcgacaatgt cggcgggatc ggcgagacgg cccatccccg
      841 actcaccgga agcgagagct ccctcgacgg gtccgatgag cttgatccgg ccgtcggcca
      901 gcagcgtggc gacgttgcgc tgcgtcgccg ggtgccgcca catgcgggtg tgcatggccg
      961 gcgcaaccag tacggggcat gccgcagata gagcgagcgc cgcgacgatg tcatcggctc
     1021 ggccctgcgc caggcgcgcg agcgtatcgg cggttgccgg agcgatgaga acgaggtcga
     1081 ccttgcgccc gagatcgacg tgcacttcac ccgggtaaga ggtggcgaac atgtcccgga
     1141 cgacgggcgt gccgcagatc cccgacaacg tgagcggccc cacgaaccgc tcggcagacg
     1201 atgtcatcac cggcgtgacg gccacacctg cggcgatgag acggcgcgcg acctcgaccg
     1261 ccttgtaggc ggcgatgctt cccgaaatcg cgagagcaac gctggccatc acgggaggtt
     1321 agcgcaggtg ccttcccgcg gctcgggagc cttggcggcg gcgtgccggc tccccttgtc
     1381 gcggactccg acgcgacgtc tcgtcgtcgt cgcctgccgg gcaatcgccg gtgcaggttg
     1441 cgtcggaatc cttggcgcga cgcttcgcca cgtagtgcga gaagccggcc ttctccgtcc
     1501 agccgtactc tccggtcttg ccgggctcgc gcacctcgac catccctcct ccgccgcgcc
     1561 ggctcccggg gtcgagcgtc gtgcccgcgg cgcctttcac gtcgggcccc tgcaagagca
     1621 cgaggtagtc gtcggcgacg tactccgggt gcggatcgtc ggtcacggca tcggcgtctc
     1681 ctccctcggc ggttcccacg cgaacgatct ggttggtggg gggatagctg tcgttccacc
     1741 tttcacgaac ggcgaaggcg ccatcgcgga cgatgcggta gcgatgggtg tgaaaccccg
     1801 ggatcccccg ctgggagagc acgcgcgcgc ccctgggaag cttgggatct tgtctctcga
     1861 gctcggcgaa gggaacgact tcg
//
LOCUS       DASVII010000647         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_533156,
            whole genome shotgun sequence.
ACCESSION   DASVII010000647 DASVII010000000
VERSION     DASVII010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533156"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VF309_08385"
     CDS             complement(<1..348)
                     /locus_tag="VF309_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX7466645.1"
                     /translation="MTLHRFTFRAMAAENEIQVHCDDPSHAGDAASRAIAEVQRIEAK
                     YSRYRAASVVSRINAQAGAAPVAIDVETRGLLAFAAACHRQSDGAFDATSGVLRRAWR
                     FDVNRLPDEGELAP"
     gene            complement(355..1524)
                     /locus_tag="VF309_08390"
     CDS             complement(355..1524)
                     /locus_tag="VF309_08390"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX7466646.1"
                     /translation="MAATEEPAGSARESRALAALMKAALALPILVVPAEAGAADVGEI
                     GITVLGYKERGLMKVTEPVLWGRAQFLEAWEVQASAAIDIISGASPQLVTNANGKPVN
                     SISGASITDRRNTGDVKVTRRFGDFALSASRTLSKENDYHSKAFGLEGRLDLNERNTT
                     LTAGYGKSDDHVLSRDDPHLNQPRDTREYLVGITQILSPTALVQSTLTTARGEGWYND
                     PYKLTFTFYPSGPPFLMSDVRPDHRDTWSWLTRYRQHFPEAHGTLQAEYRFYRDDWGI
                     RAHTIELAWQHEVNERWSVRPALRYYTQDAADFYSPVIPVSPPAILSSDQRLGAFGGL
                     SPSLRAIVRLADGVVVEGTVGYMQNARNFHFGSSGSEAFETLRAVYGIIGVRRAF"
     gene            complement(1511..1723)
                     /locus_tag="VF309_08395"
     CDS             complement(1511..1723)
                     /locus_tag="VF309_08395"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEX7466647.1"
                     /translation="MRFAFLAATLCALCASGCTAVAPYQRGYLARQDMAFDADPGTMK
                     SIEKTFAAKEAASGGAAVGGGGCGCN"
BASE COUNT          271 a          594 c          622 g          285 t
ORIGIN      
        1 cggcgcgagc tcgccctcgt cgggcaggcg gttcacgtcg aagcgccacg cgcgtctcag
       61 cacgcccgag gtcgcgtcga atgccccgtc gctctggcga tggcacgccg ccgcgaaggc
      121 gagcaatccg cgggtctcga cgtcgatcgc aacgggggcg gcgccggcct gcgcgttgat
      181 gcgcgagacc acgctcgcgg cgcgatagcg cgagtacttc gcctcgatgc gctgcacctc
      241 cgcgatggcg cgcgaggccg cgtccccggc atgcgaggga tcgtcgcagt gaacctggat
      301 ctcgttctcc gcggccatcg cgcggaaggt gaagcgatgc agcgtcatgg cgcctcagaa
      361 ggcgcggcgc actccgatga ttccgtagac cgcgcgcagc gtctcgaagg cttcgctccc
      421 cgagctcccg aagtggaagt tgcgcgcgtt ctgcatgtag cccacggtgc cctcgaccac
      481 aacgccgtcc gcgaggcgaa cgatcgcccg aagcgagggc gacagcccgc cgaaggcgcc
      541 gaggcgctgg tcgctcgaca ggatcgccgg aggcgacacg ggaatgacgg gagagtagaa
      601 gtcggcggca tcctgggtgt agtagcgcag cgccggcctc accgaccagc gctcgttgac
      661 ctcatgctgc cacgccagct cgatcgtgtg ggcgcgaatc ccccagtcgt cgcgatagaa
      721 gcggtactcg gcctgcagcg tcccgtgcgc ctcgggaaag tgctgccggt agcgcgtgag
      781 ccaggaccac gtgtcgcgat gatcggggcg cacgtcggac atgaggaacg gcggcccgct
      841 gggatagaac gtgaacgtga gcttgtaggg gtcgttgtac cagccttcgc cccgcgccgt
      901 ggtcagcgtg ctctgcacga gggccgtggg cgagagaatc tgcgtgatgc cgacgaggta
      961 ttcgcgagtg tcgcggggct ggttgagatg cggatcgtcc cgggaaagga cgtggtcgtc
     1021 ggacttgccg tagcccgcgg tgagcgtggt gttgcgctcg ttcaggtcga ggcgcccttc
     1081 caggccgaac gccttggagt ggtaatcgtt ttccttcgac agcgtccgcg acgcggacag
     1141 cgcgaagtcg ccgaaacggc gcgtcacctt cacgtcgccc gtattgcggc gatcggtaat
     1201 ggaggcgccg gaaattgaat tgaccggctt gccgtttgcg ttcgtcacga gctggggcga
     1261 cgcgccgctg atgatgtcga tcgccgcgct tgcctggact tcccatgctt cgaggaactg
     1321 ggcgcgtccc cagaggacgg gctcggtcac cttcatcagg ccgcgctcct tgtatccgag
     1381 cacggtgatg ccgatctcgc ccacgtccgc cgcgccggcc tcggcgggaa ccacgaggat
     1441 cggcagcgcg agcgccgcct tcatcagggc ggcgagcgcg cggctttccc gggcggaccc
     1501 ggcgggctcc tcagttgcag ccacatccgc ctccgccgac cgccgcgccc ccgctcgccg
     1561 cttccttcgc ggcgaaggtc ttctcgatcg acttcatcgt tccgggatcg gcgtcgaagg
     1621 ccatgtcctg gcgcgcgagg tagccgcgct ggtacggcgc cacggccgtg catccgctcg
     1681 cgcagagcgc gcacagcgtc gcggcaagaa aagcgaatct catgaaggct ccttcaacag
     1741 cgcgtcgatc tcgcgctcga gcgccgcggc cg
//
LOCUS       DASVII010000648         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_461396,
            whole genome shotgun sequence.
ACCESSION   DASVII010000648 DASVII010000000
VERSION     DASVII010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461396"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VF309_08400"
     CDS             <1..441
                     /locus_tag="VF309_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7466648.1"
                     /translation="VAELEAHLRTIHAPFGKLTDAQWRHIAETSAVKTEEGDFRQHYD
                     PGIGRAFSWPLMVDISLWHVWDHVACPVMILRGEDSDLLHASTVREMQKRGVSGKNGL
                     VRSVEVRGVGHAPALMNDSQITLIEDFLADQKVTTKALKVAGLK"
     gene            438..2393
                     /locus_tag="VF309_08405"
     CDS             438..2393
                     /locus_tag="VF309_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HEX7466649.1"
                     /translation="MKNKVVSAAEAIAIVRDGDTIAFSGFVGTGTPDELIGELEKRFL
                     ETGHPRDLTLVFAAAPGDGKDRGLNKLAHPGLVKRVVGGHWSLVPKLGAMAVANEIEA
                     YNLPLGVISHLFRDIGAHRAGAITKVGLRTFVDPRQGGGRLNARTKDALVRVMQIEGE
                     EWLFYKAFPITVAFLRGTTADLAGNITMEHEALVLDNLAIATAAKNSGGFVIVQVERV
                     CAHGALHSRDVVVPGVMVDCAVVAQPHNHHQTYGTQYSPAFAGEYRVALDMLAPMPLD
                     ERKIIARRAAFELPMGGVVNLGIGMPEGVAAVANEERVLGHLTLTAEPGVIGGVPQSG
                     LDFGAAVNTEVVIHQNQQFDFYDGGGLDMACLGMAEVDAEGNVNVSRFGPKLAGAGGF
                     INISQSARKLVFVGTFTAGGLRVQIDGTDLVIVKEGSTRKFVKAVEQITFNGAYAAEK
                     GQPVLYVTERCVFRRTHDGMELAEVAPGIDIERDILAHMDFVPIVKNPITMDPCIFAD
                     LPMLLDETLLGLGLAQRVSYDAERNVLFLNMEGMHVKTRDDVDRVRRVIEERVQAIGQ
                     KVAFVANYDGLKIDAAVVDTYAAMVRYLETNYYTTASRYTTSAFMRVKLGEAMSRRQV
                     APHIFETREEAHAFVSARDKESLPESE"
BASE COUNT          422 a          803 c          810 g          363 t
ORIGIN      
        1 gtcgccgagc tcgaggccca cttgcgcacg atccacgcgc cattcggcaa gctgaccgat
       61 gcgcagtggc gccacatcgc cgagaccagc gccgtgaaga ccgaggaagg cgacttccga
      121 cagcactacg atccgggcat cgggcgcgcc ttctcgtggc cgctcatggt ggacatctcg
      181 ttgtggcacg tgtgggacca cgtcgcatgc ccggtgatga tcctgcgcgg cgaggattcc
      241 gacctcctgc atgcgtccac cgtgcgcgag atgcagaagc gcggagtctc cgggaagaac
      301 ggcctggtgc gttccgtgga ggtgcgcggc gtgggccacg cgcccgcgct gatgaacgat
      361 tcgcagatca cgctcatcga ggacttcctc gccgaccaga aggtgacgac gaaggccctc
      421 aaggtcgcgg gcctcaagtg aagaacaagg tcgtctccgc cgcggaggcc atcgcgatcg
      481 tccgcgacgg cgacaccatc gccttctcgg gattcgtcgg caccggcacg cccgacgagc
      541 tgattggcga gctggagaag cgcttcctcg agacggggca cccacgcgat ctcacgctgg
      601 tcttcgccgc ggcgccgggc gacggcaagg accgcggact gaacaagctc gcccatccgg
      661 gattggtgaa gcgcgtggtg ggaggccact ggtcgctcgt gccgaagctg ggcgcgatgg
      721 cggtggcgaa cgagatcgag gcctacaacc tgccgctcgg cgtgatcagc cacctcttcc
      781 gcgacatcgg cgcccatcgg gcgggcgcca tcaccaaggt ggggctgcgc acgttcgtcg
      841 atccgcgcca gggcggaggg cggctgaacg cgcgcacaaa ggacgcgctg gttcgcgtga
      901 tgcagatcga gggcgaggag tggctgttct acaaggcgtt cccgatcacc gtcgcgttcc
      961 tgcgcggcac cacggccgat ctcgccggaa acatcacgat ggaacacgag gcgctggtcc
     1021 tcgacaacct cgccatcgcc accgccgcca agaattccgg cggcttcgtg atcgtgcagg
     1081 tggagcgcgt gtgcgcgcac ggcgcgctcc actcgcgcga tgtcgtggtg cccggcgtga
     1141 tggtcgattg cgcggtggtg gcgcagcccc acaaccatca ccagacatac ggcacgcaat
     1201 acagccccgc cttcgccggc gaataccgcg tggcgctcga catgctcgcg ccgatgccgc
     1261 tcgacgaacg caagatcatc gcgcggcgcg cggccttcga gctgcccatg ggcggcgtcg
     1321 tgaacttggg catcggcatg cccgaaggtg tcgccgcggt cgcgaacgag gagcgcgtgc
     1381 tcggccacct cacgctcacc gccgagcccg gcgtgatcgg cggcgtgccg cagtcggggc
     1441 tcgacttcgg cgccgccgtg aacaccgagg tcgtcatcca ccagaaccag cagttcgatt
     1501 tctacgacgg cggcggcctc gacatggcgt gcctcggcat ggccgaggtc gatgccgagg
     1561 ggaacgtcaa cgtgagccgc ttcgggccca agctcgcggg cgccggcggc ttcatcaaca
     1621 tctcgcagag cgcgcgcaag ctcgtgttcg tggggacctt caccgcgggc ggcctgcgcg
     1681 tgcagatcga cggcaccgac ctcgtcatcg tgaaggaggg cagcacccgc aagttcgtga
     1741 aggccgtcga gcagatcacg ttcaacgggg cgtacgccgc cgagaagggc cagccggtgc
     1801 tctacgtgac ggagcgctgc gtctttcgcc gcacccacga cggcatggag ctcgcggaag
     1861 tggcgcctgg catcgacatc gagcgcgaca tcctcgcgca catggacttc gtgcccatcg
     1921 tcaaaaatcc catcacgatg gatccatgca tcttcgccga ccttccgatg ctgctcgacg
     1981 agacgcttct gggcctcggg ctcgcgcagc gcgtgagcta cgacgccgag cgcaacgtgc
     2041 tcttcctcaa catggaaggc atgcacgtga agacgcgcga cgacgtcgac cgcgtgcgcc
     2101 gcgtgatcga ggagcgcgtg caggcgatcg gccagaaggt cgcgttcgtc gccaactacg
     2161 acgggctcaa gatcgacgcg gcggtcgtcg acacctatgc ggcgatggtt cgctacctcg
     2221 agaccaacta ctacaccacc gcgtcgcgct acaccacgag cgccttcatg cgcgtgaagc
     2281 tgggcgaggc gatgtcgagg aggcaggtgg ccccgcacat cttcgagacg cgcgaagagg
     2341 cgcatgcgtt cgtctccgcg cgcgacaagg aatcgcttcc ggagagcgaa tgagacac
//
LOCUS       DASVII010000649         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_425521,
            whole genome shotgun sequence.
ACCESSION   DASVII010000649 DASVII010000000
VERSION     DASVII010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425521"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VF309_08410"
     CDS             complement(<1..383)
                     /locus_tag="VF309_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466650.1"
                     /translation="MKRLQALAFLFAAAGFSSAALAQTKWDMPTPYAPTNFHTENINQ
                     FATDVDKATGGKLKITVHSNASLFKANEIKRAVQGGQAQIGEILISGYSNEDPVFGVD
                     SVPFLATSYADAAKLWKASKKALEDR"
     gene            447..>1802
                     /locus_tag="VF309_08415"
     CDS             447..>1802
                     /locus_tag="VF309_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7466651.1"
                     /translation="MIPRNRAWQFWIDRGGTFTDIVARKPDGSLATHKLLSENPERYR
                     DAAIAGIRELLGVAPGAPIPVEKIEAVKMGTTVATNALLERKGERTVLFITRGFRDQL
                     RIAYQNRPRIFDRHIKLPELLYTKVVEVDERLGARGEVIVALDERAVRRDLESARAAG
                     YRSIAIIFMHGYRYPAHEAAVARLANWTGFDQVSASHGVSPLMKIVSRGDTTVVDAYL
                     SPILRRYVDQVAAELPGAHLLFMQSNGGLTDAHRFQGKDSILSGPAGGIIGMVRTSEA
                     AGFPKVIGFDMGGTSTDVSHYAGELERELETLVAGVRMRAPMMSIHTIAAGGGSILFF
                     DGQRQRVGPDSAGANPGPASYRRGGPLTVTDCNVMLGKIQPAFFPKVFGPGGDEALDA
                     EVVREKFAELSMQIATATARPQTREAVAEGYIRIAVANMANAIKFISVQRGHDVTEYT
                     LA"
BASE COUNT          293 a          603 c          616 g          290 t
ORIGIN      
        1 cggtcctcga gcgctttctt cgaggctttc cagagcttcg cggcatcggc atagctggtc
       61 gcgaggaacg gcaccgagtc cacgccgaac accgggtcct cgttggagta cccggagatg
      121 aggatctcgc cgatctgcgc ctgcccgccc tgcaccgcgc gcttgatctc gttcgccttg
      181 aagagcgacg cgttggaatg cacggtgatc ttgagcttgc ccccggtcgc cttgtccacg
      241 tcggtggcga actggttgat gttctcggtg tggaaattgg tgggcgcgta gggcgtgggc
      301 atgtcccact tggtctgcgc gagggcggcg gacgagaatc ccgcggccgc gaataggaag
      361 gcgagtgcct ggaggcgctt catggttttt ctccttgggt agggaccgaa cctaacaaag
      421 ggcggggtgc atgtcaaaat cacgagatga tcccccgtaa ccgcgcctgg cagttctgga
      481 tcgaccgcgg tggcacgttc accgacatcg tcgcgcgcaa gcccgacggc agcctcgcca
      541 cgcacaagct cctctcggag aatcccgagc gctaccgcga cgccgccatc gccggcatcc
      601 gcgagctgct cggcgtcgcg cccggcgcgc cgatcccggt cgagaagatc gaggcggtga
      661 agatggggac cacggtcgcc accaacgcgc tcctcgagag gaagggcgag cgcaccgtgc
      721 tcttcatcac gcgcggcttc cgcgaccagc tgcgcatcgc ctaccagaac cggccgcgca
      781 tcttcgaccg ccacatcaag ctgcccgagc tcctgtacac gaaggtggtg gaggtggacg
      841 agcgcctggg cgcccgcggc gaggtgatcg tggcgctcga cgagcgcgcg gtgcgccgcg
      901 atctcgagag cgcgcgcgcc gcgggctacc gctccatcgc gatcatcttc atgcatggat
      961 atcgctatcc ggcccatgag gccgcggtcg cccggctcgc gaactggacg ggcttcgacc
     1021 aggtctccgc ctcccacggg gtgtcgccgc tcatgaagat cgtctcgcgc ggcgacacca
     1081 ccgtggtcga cgcgtacctt tcaccgatcc tgaggcgata cgtcgaccag gtggcggcgg
     1141 agctgccggg cgcgcacctc ttgttcatgc agtcgaacgg cggcctcacc gacgcccatc
     1201 gtttccaggg gaaggactcg atcctctccg gccccgcggg cggcatcatc ggcatggtgc
     1261 gcacctccga ggccgcgggc ttcccgaaag tgatcggctt cgacatgggc ggcacctcga
     1321 ccgacgtgtc gcactacgcg ggagagctcg agcgcgagct ggagacgctg gtcgcgggcg
     1381 tgcgcatgcg cgcgccgatg atgtcgatcc acacgatcgc cgcgggcggc ggctcgatcc
     1441 tcttcttcga cgggcaaagg cagagggtgg gccccgattc cgcgggggcg aatcccggac
     1501 ccgcgagcta ccggcgcggc gggccgctca cggtgaccga ctgcaacgtg atgctcggca
     1561 agatccagcc cgcgttcttc ccgaaggtgt tcgggccggg gggcgacgag gcgctcgatg
     1621 ccgaggtcgt gcgcgagaag ttcgccgagc tctcgatgca aatcgccacg gccaccgcgc
     1681 ggccccagac gcgggaagcc gtcgccgagg gatatatccg catcgcggtg gcgaacatgg
     1741 cgaacgcgat caagttcatc tcggtgcaac gcgggcacga cgtgacggaa tacacgctcg
     1801 cg
//
LOCUS       DASVII010000650         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_173552,
            whole genome shotgun sequence.
ACCESSION   DASVII010000650 DASVII010000000
VERSION     DASVII010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173552"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VF309_08420"
     CDS             <1..397
                     /locus_tag="VF309_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7466652.1"
                     /translation="YLGRGQFLANDMGQLAHAAVFMIGVAYAWRWDRQVRVDLLYRTS
                     NARRRAFVNLLGTVFLLLPWLVLVTWDAVPDVIESVRNVEGFPETGSPGFFVMKSLIV
                     AFAAMMSLQATAVIARSIVVIRDPSRARA"
     gene            394..1782
                     /locus_tag="VF309_08425"
     CDS             394..1782
                     /locus_tag="VF309_08425"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7466653.1"
                     /translation="MILSILREHLGLLMLATLVVLLTTGFPVAFTLMGTAVGFALLGN
                     ALGFFDAHWLSALSLRVLGLMNDDLLQAVPVFIYLGVILQRTTLSTDLLETMAGLFGS
                     RAGGLGISSIVLGVLLAPTTGVVGATVLTIGLLTLPSMLEAGYDRRFASGIVVCAGTL
                     GTIIPPSIILIMLSGQMRLAARETVVPGGGSVSVNFLQIFVGVLLPVAILLCLYLAYV
                     LGVALLAPRAAPAIPSHGRRPLTLRRVVVSLVLPLGLIGLMLASILSGKLYTVEAAAI
                     GAIAITIYTLLRRELTWARLGETVREVMRLSAVVFTLMMGAMTFTLVFRIMGGDVGVT
                     KALASVPGGLHGATLLVLGLSLGLCLLLDALEILFVVVPLTMPTLLGLGGDPVWLAVM
                     FAITIQSGFMMPPSGFAICFLRSVAPAQVQTREIYLGVVPLLAIQVTVLTLLWTFPAI
                     ATWLPKVLYGAP"
BASE COUNT          232 a          681 c          616 g          371 t
ORIGIN      
        1 gtacctgggc cgcggccagt tcctcgccaa cgacatgggc cagctcgcgc acgccgcggt
       61 cttcatgatc ggggtcgcgt acgcatggcg ctgggaccgg caggtgcgcg tggacctcct
      121 ctatcggacc tcgaacgcgc ggcgcagggc gttcgtgaat ctcctcggca ccgtcttcct
      181 gctgctgccg tggctcgtcc tcgtcacctg ggacgccgtg cccgacgtga tcgaatcggt
      241 gcgcaacgtc gagggatttc cggagaccgg atcgcccgga ttcttcgtga tgaaatcgtt
      301 gatcgtggcg ttcgccgcga tgatgtcctt gcaggcgacc gcggtcatcg cgcgctccat
      361 cgtggtcatc cgggatccgt cgcgcgcgcg ggcatgatcc tgtcgatcct gcgcgagcac
      421 ctgggcctcc tgatgctggc cacgctcgtc gtgctcctca cgacgggctt tcccgtcgcc
      481 ttcacgctga tgggcaccgc cgtcggcttc gcgctcctcg gcaacgcgct cggcttcttc
      541 gacgcgcact ggctctcggc gctctcgctg cgcgtgctcg gcctcatgaa cgacgacctg
      601 ctgcaggcgg tgccggtgtt catctacctc ggcgtgatcc tgcagcgcac gacgctttcc
      661 acggacttgc tggagaccat ggcgggcctc ttcggctcgc gcgccggggg cctgggcatc
      721 tcgagcatcg tgctcggcgt gctgctcgcg ccgaccacgg gggtcgtcgg cgccacggtg
      781 ctcacgatcg ggctcctcac tttgccgagc atgctcgaag ccggctacga ccgccgcttc
      841 gcgagcggca tcgtggtctg cgcgggaacc ctcgggacca tcatcccgcc gtcgatcatc
      901 ctcatcatgc tgtccgggca gatgcggctc gccgcgcgcg agacggtggt gccgggcggc
      961 ggctccgtca gcgtgaactt cctgcagatc ttcgtcggcg tgctgctgcc cgtcgcgatc
     1021 ctgctgtgcc tgtacctcgc ctacgtgctc ggcgtggcgc tcctcgcccc gcgcgcggca
     1081 cccgcgattc catcccatgg gcggcggccc ctgacgttgc gtcgcgtggt cgtgagcctc
     1141 gtgctgcccc tcggattgat cgggctcatg ctcgcctcga tcctgagcgg gaagctctac
     1201 accgtggaag cggccgccat cggcgccatc gcgatcacga tctacaccct gctgcgccgc
     1261 gaactcacct gggcgcgcct cggcgaaacc gtgcgcgaag tgatgcgcct ttccgccgtg
     1321 gtcttcacgc tgatgatggg cgcgatgacc ttcacgctgg tcttccgcat catgggcggg
     1381 gacgtcggcg tgacgaaggc cctcgcgtcg gtcccgggcg ggctccacgg cgcgacgctc
     1441 ctggtcctgg gcctgagcct cgggctttgc ctgctcctcg acgcgctcga gatcctgttc
     1501 gtcgtggtcc cgctcaccat gccgacgctc ctcggcctgg gcggcgaccc ggtgtggctc
     1561 gcggtgatgt tcgcgatcac gatccagagc ggcttcatga tgccgccctc gggcttcgcg
     1621 atctgcttcc tgcgcagcgt cgcgcccgcg caagtgcaga cgcgcgagat ctatctcggc
     1681 gtcgtgccgc tcctcgcgat ccaggtcacg gtcctcacgc tactctggac attcccggcg
     1741 atcgcgacat ggcttccgaa ggtgctctat ggagctccct agtcgcgcgc cgcgcccgac
     1801 tgatcgcata atgtgggcgg agcgaaggcc gccccatcgc agccacgaca ggagagacat
     1861 cgatgctcga tagaacgctc ggcaccgtcg catgcgtcct
//
LOCUS       DASVII010000651         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_390002,
            whole genome shotgun sequence.
ACCESSION   DASVII010000651 DASVII010000000
VERSION     DASVII010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390002"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VF309_08430"
     CDS             complement(<1..1263)
                     /locus_tag="VF309_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466654.1"
                     /translation="MSARALRRVLARAAVALACAAATLGAASAQITVQHIHADMQVLV
                     DKFMREQPGTRVDFRLVSFQSILESLPVQLASGLGPDVSMVADWGGLAKYYLDLRPYV
                     DAGYFEREFAMLLDPMRGGVAGSNAINGMSGSMTLTGAFVNVTLFKQAHVALPGEGAT
                     WDDWAAAARKVAKATRTEMPMEMDRSGHRFASLAISYGAKLVDDDGNPVVDSGLKTAI
                     RKFVAWHRDNTMPMDLWGAVGGATHRDNFQDFMNGKVVFYFGGSWSLFKMDSVVGDLF
                     EWVVVDAPCGTSSCTLMPGGGAMVAFKHTRNPRLAAKFLGFLAREDNMREALARHPNI
                     PVATTLVRTGVDYPGASERVRAGLESFTRQVPAIPKAAYAFQGWRYQRAAMNAMTTRI
                     SQVLTYELDVDTAAAFIKKDVELAIQAAR"
     gene            complement(1260..>1850)
                     /locus_tag="VF309_08435"
     CDS             complement(1260..>1850)
                     /locus_tag="VF309_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7466655.1"
                     /translation="ICAIGIDDYRAAHEMTQHLITLGHARIGFIAGHPNQTASARRLA
                     GFRAAMEEAHLEVPEELVAQGAFNYRSGLDAAEVLLGKSERPSAIFASNDDMAAATVA
                     IAHRLGLDVPGDLTVAGFDDAALATTIWPELTTVRQPINEMAGAAVDLLVRQIRARRE
                     GLDEACEHVVMDFSLIRRQSDAAPRVRPPIAIARQA"
BASE COUNT          266 a          634 c          661 g          289 t
ORIGIN      
        1 cctggcggcc tggatggcca gctcgacgtc cttcttgatg aaggccgccg ccgtgtccac
       61 gtcgagctcg taggtgagca cctggctgat gcgggtggtc atggcgttca tcgccgcgcg
      121 ctggtagcgc cagccctgga acgcgtacgc ggccttcggg atcgcgggca cctgcctcgt
      181 gaagctctcg agccccgcgc gcacccgctc cgacgcgccg ggatagtcga cgccggttct
      241 cacgagcgtc gtggccaccg gaatgttggg atggcgggcg agcgcctcgc gcatgttgtc
      301 ctcgcgcgcc agaaagccga ggaacttcgc ggcgagccgc gggttgcggg tgtgcttgaa
      361 cgccaccatc gcgcctccgc ccggcatcaa ggtgcaggac gacgtcccgc acggcgcgtc
      421 caccaccacc cactcgaaga gatcgccgac gacgctatcc atcttgaaca acgaccacga
      481 tccgccgaaa tagaagacga ccttgccgtt catgaagtcc tggaagttgt cgcgatgcgt
      541 cgcccctcct acggcgcccc acaggtccat gggcatcgtg ttgtcccggt gccatgcgac
      601 gaatttccgg atggcggtct tcagccccga gtcgaccacc gggttgccgt cgtcgtccac
      661 gagcttcgct ccgtagctga tggcgaggct cgcgaagcgg tggccggagc ggtccatctc
      721 catcggcatc tcggtgcgcg tggccttcgc gaccttgcgc gcggccgcgg cccagtcgtc
      781 ccaggtggcg ccctcccccg gcagcgcgac gtgcgcctgc ttgaagagcg tcacgttcac
      841 gaaggcgccg gtaagcgtca tcgagccgga catgccgttg atggcgttgc tgcccgcgac
      901 gccgccgcgc atcggatcga ggagcatcgc gaattcgcgc tcgaagtagc ccgcgtccac
      961 gtaggggcgc aggtcgaggt agtacttcgc gagcccgccc cagtccgcga ccatcgagac
     1021 gtccggcccg aggcccgagg cgagctgcac cggcaggctc tcgaggatgg attgaaagga
     1081 gaccaggcgg aagtcgacgc gcgtgccggg ctgctcgcgc atgaacttgt ccaccagcac
     1141 ctgcatgtcg gcgtggatgt gctggacggt gatctgcgcg ctcgcggcgc cgagcgtggc
     1201 ggcggcgcac gcgagggcga cggcggcgcg cgcgaggacc cggcgcaacg cgcgagcgct
     1261 caagcttgtc tcgcgatcgc gatgggcggg cgcacgcgcg gtgcggcgtc cgactggcgg
     1321 cggatgaggg agaaatccat caccacgtgc tcgcaggcct cgtccaggcc ctcgcggcgc
     1381 gcgcggatct ggcgcacgag aaggtcgacc gcggcgccgg ccatctcgtt gatcggctgg
     1441 cgcacggtgg tgagctcggg ccagatggtg gtggcgagcg cggcgtcatc gaacccggcg
     1501 acggtgaggt cccccggcac gtcgagcccg aggcggtggg cgatggccac cgtggccgcc
     1561 gccatgtcgt cgttgctcgc gaagatcgcg ctcgggcgct cgctcttgcc gaggagcacc
     1621 tcggccgcgt ccagccccga tcggtagttg aaggcgcctt gcgcgaccag ctcctcgggc
     1681 acctcgaggt gcgcctcctc catcgcggcg cggaacccgg cgaggcggcg cgcgctcgcc
     1741 gtctggttcg ggtgcccggc gatgaagccg atgcgcgcgt gccccagcgt gatgaggtgc
     1801 tgcgtcatct catgggccgc gcggtagtcg tcgatgccga tggcgcagat
//
LOCUS       DASVII010000652         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_173805,
            whole genome shotgun sequence.
ACCESSION   DASVII010000652 DASVII010000000
VERSION     DASVII010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173805"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="trpD"
                     /locus_tag="VF309_08440"
     CDS             complement(<1..947)
                     /gene="trpD"
                     /locus_tag="VF309_08440"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7466656.1"
                     /translation="MFTPLEAINRLCDKREIFYDEMVDLMRQVMEGKVPPVQLAAILM
                     GLHVKTESVSEIAAAASVMREFSTKVDSEGLDHLVDTCGTGGDKAHTFNISTTAAFVA
                     AAGGARVAKHGGRSVSSQSGSADVLEALGVNLALTPDQVAQSLREVGVGFMFAPNHHP
                     AMKHAAPVRKDLGMRTILNILGPLTNPAGAPNQVMGVFHPDLVGIQARVLKMLGSRHV
                     LTVHGHEGLDEITITGPTYVAELKHDFITEYTIEPKQFGLDVAPIESIQVKDAADSKA
                     RVEAVLANEPGPSRDVVILNAAAALYVAGVVPSLWDGVAA"
     gene            complement(953..1516)
                     /locus_tag="VF309_08445"
     CDS             complement(953..1516)
                     /locus_tag="VF309_08445"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7466657.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGEDVKVIRNDELTVDGIEALAP
                     GRIVLSPGPCTPNEAGVSLELIARFAGRVPILGVCLGHQAIGQAFGGKIVHAKQVMHG
                     KTSAIRHHSTSVFRNLPNPLQATRYHSLVIERASLPDCLEVTAWTEDGEIMGVRHRSL
                     PIEGVQFHPESILTQHGHALLSNFLKG"
BASE COUNT          254 a          498 c          533 g          278 t
ORIGIN      
        1 gcggcgactc cgtcccagag gctcggcacg acacccgcga cgtagagcgc ggcggccgca
       61 ttgagaatga ccacgtcgcg cgacggcccg ggctcgttcg cgaggaccgc ctccacgcgc
      121 gcctttgaat cggccgcgtc cttcacctgg atggattcga tgggcgcgac gtcgagtccg
      181 aactgcttcg gctcgatggt gtactccgtg atgaagtcgt gcttgagctc cgcgacgtag
      241 gtggggccgg tgatcgtgat ctcgtcgagc ccttcgtggc cgtgcacggt gaggacatgg
      301 cggctgccga gcatcttcag gacgcgggcc tggatgccga cgagatccgg atggaagact
      361 cccatcacct ggttgggcgc accggcggga ttggtgagcg gcccgaggat gttgaggatc
      421 gtgcgcatgc cgaggtcctt gcggaccggc gcggcgtgtt tcatcgcggg atgatggttc
      481 ggcgcgaaca tgaatccgac gcccacctcg cgaagcgact gtgcgacctg gtcgggcgtg
      541 agcgcaagat tcacgccgag ggcctcgagg acgtccgcgc ttcccgactg cgaggaaacc
      601 gagcggccgc cgtgcttcgc cacgcgcgcg ccgcccgccg ccgccacgaa cgccgcggtc
      661 gtggagatgt tgaaggtgtg cgccttgtcc ccgccggtgc cgcaggtgtc gacgagatgg
      721 tcgagaccct cggagtcgac cttcgtcgag aactcgcgca tcaccgaagc cgcggccgcg
      781 atctcggaga ccgattccgt cttcacgtgc agccccatga ggatcgcggc gagctgcacc
      841 ggcggcacct tgccctccat cacctggcgc atgagatcga ccatctcgtc gtagaagatc
      901 tcgcgcttgt cgcagaggcg gttgatcgct tcgagcggcg tgaacatcgg cttcatcctt
      961 tgagaaagtt gctgagcagg gcgtggccgt gctgcgtgag tatcgattcg gggtggaact
     1021 gcacgccctc gatcggcagc gagcgatggc gaacgcccat gatctcgccg tcctcggtcc
     1081 acgcggtgac ttccaggcaa tccggcaggc tcgcgcgctc gataacgagc gaatggtagc
     1141 gcgtcgcctg cagcggattc ggcagattgc gaaaaacgct cgttgaatgg tggcggatcg
     1201 cggaagtctt gccgtgcatc acctgtttcg cgtgcacgat tttgccgccg aacgcctgcc
     1261 cgatcgcctg gtgcccgagg cacacgccga ggatgggaac gcgacccgcg aagcgcgcga
     1321 tgagctcgag ggaaacgccc gcttcgttcg gcgtgcacgg ccccggcgaa aggacgatgc
     1381 gcccgggcgc gagcgcctcg atcccgtcga cggtgagctc gtcgttgcgg atcaccttca
     1441 cgtcctcgcc cagctcgccg aggtattgca ccaggttgta ggtgaaggag tcgtagttgt
     1501 cgatcatgag cagcatgatc agtcctcaag cctgctgtcc agcccggctg cggccatttc
     1561 cgc
//
LOCUS       DASVII010000653         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_246645,
            whole genome shotgun sequence.
ACCESSION   DASVII010000653 DASVII010000000
VERSION     DASVII010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246645"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VF309_08450"
     CDS             <1..1160
                     /locus_tag="VF309_08450"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX7466658.1"
                     /translation="CASAGHHLESALAAHGSGAAALSRDERKAVLNVDIGGGTTKLAL
                     CVAGEVVSTCAFAVGGRLVAFDGEGVVTRVDEAAALAAADAGVQLRLGCRPAPTDLAR
                     VVDALAEAAIAFIRGATPIALGEKLALTETLALAAAPDAITFSGGVSEYIYGRETRQF
                     GDIARLLAERIAQACADGRIEPPLAVPREGIRATVIGASQFTVQVSGKTIHVSAGVSL
                     PLRNVAVAAPALTLTEDIDVAREAHAIELAVARSCADGAHAIALSIRWRGDPHYRRLR
                     ALGEAIALAMASASDAPSQRLAPLVLMIDGDVGRTLGHILEHDLAIGRALVSIDGIAL
                     KEFDFVDIGEMIRPADVVPVVIKSLLFSGAPARASHGAGASYKPLTERKVQ"
     gene            1157..>1559
                     /locus_tag="VF309_08455"
     CDS             1157..>1559
                     /locus_tag="VF309_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466659.1"
                     /translation="MNAPESPRVAVDVYLEWLKKEGIPVSEDFGVDLLKVPTGRWGRY
                     GVDAAAVHLKGRGDFLCMFVFDIPGSGATAPQRHLYEEVFYVLEGRGSTTIEAPDGRT
                     HSFEWGPRSLFAIPLNTRYRLFNGSGSERARL"
BASE COUNT          220 a          559 c          539 g          241 t
ORIGIN      
        1 tgtgcgcttc cgccgggcat catctggaga gcgccctggc cgcccacggc tcgggcgccg
       61 ccgccctctc gcgcgatgaa cgcaaggcgg tcctcaacgt cgacatcggc ggcggcacga
      121 ccaagcttgc gctgtgcgtc gcgggcgagg tcgtctcgac gtgtgcgttc gcggtcggcg
      181 gccgcctggt cgccttcgac ggcgagggcg tcgtcacgcg ggtcgacgag gccgccgcgc
      241 tcgccgcggc ggacgccggc gtgcagctgc gattgggatg ccgtcccgct cccaccgacc
      301 tcgcgcgggt cgtcgatgct ctcgccgaag cggcgatcgc cttcattcgc ggcgcaactc
      361 cgatcgcgct cggcgaaaag ctcgccctca ccgagacgct cgcgcttgcc gcggcgccgg
      421 acgcgatcac gttctccggc ggagtctccg agtacatcta tggccgcgag acgcggcagt
      481 tcggggacat cgcccgcctc ctcgcagaac gcatcgcgca ggcgtgcgcc gacgggcgca
      541 tcgagccgcc gctcgcggtg ccgcgcgagg gtatccgcgc cacggtgatc ggcgcctcgc
      601 aattcacggt ccaggtgagc ggcaagacca tccacgtgag cgcgggcgtg tcgcttccgc
      661 tgcgcaacgt cgcggtggct gcgcccgcgc tcacgctcac cgaggacatc gacgtcgcgc
      721 gcgaggccca tgccatcgag ctcgccgtcg cgcgatcctg cgcggacggc gcccacgcga
      781 tcgcgctgtc gatccgctgg cgtggagatc cgcactatcg gcggttgcgc gcgctggggg
      841 aggcgatcgc gctcgcgatg gcttcggctt ccgacgcccc ttcgcaacgc ctcgcgcccc
      901 tggtcctgat gatcgacggc gacgtggggc gcacgctcgg ccacatcctc gagcacgacc
      961 tcgcgatcgg gcgagcgctg gtgtcgatcg acggcatcgc cctgaaggag ttcgacttcg
     1021 tcgacatcgg cgagatgatc aggcccgcgg acgtggtgcc cgtcgtcatc aagtcgctcc
     1081 tcttctccgg cgcgccggcc cgcgcatccc acggcgccgg cgcttcgtac aaacccctga
     1141 cagagagaaa agtgcaatga acgcgcccga atccccgaga gtcgcagtcg acgtgtacct
     1201 cgagtggctg aagaaggaag gcatcccggt cagcgaagac ttcggcgtgg atctcctgaa
     1261 ggtgccgacg gggcgatggg gccgctacgg agtggacgcg gcggcggtcc acctgaaggg
     1321 ccgcggcgat ttcctctgca tgttcgtctt cgacattccc ggctcgggcg cgacggcgcc
     1381 gcagcgccac ttgtacgagg aggtgttcta cgtcctcgag ggccgcggca gcaccaccat
     1441 cgaggcgccc gacgggcgca cgcactcctt cgagtgggga ccgcgaagcc tcttcgcgat
     1501 tccgctcaat acgcgctatc ggctattcaa cggcagcggc tcggagcgcg cgcggctcg
//
LOCUS       DASVII010000654         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_713886,
            whole genome shotgun sequence.
ACCESSION   DASVII010000654 DASVII010000000
VERSION     DASVII010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713886"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF309_08460"
     CDS             complement(<1..192)
                     /locus_tag="VF309_08460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7466660.1"
                     /translation="MIAIGLTGGIGSGKTTVADRLAGLGATLVDTDAIARSLTGAGGA
                     AIDGVRDAFGAAFIGADGAL"
     gene            complement(189..1055)
                     /locus_tag="VF309_08465"
     CDS             complement(189..1055)
                     /locus_tag="VF309_08465"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX7466661.1"
                     /translation="MEFLEVLRQSPAMLMVAVGLVGLCIGSLLNVVIHRLPKMMEADW
                     RDECASLDGREPTPRETYNLFTPRSACPQCKAPITALQNIPVVSWLLLRGKCARCAAP
                     ISARYPMVEILGAALAALVAWRFGYSTALAYALVYVWVMLALAFIDFDTQLLPDDLTL
                     PLLWLGLIANVAGTYTDLRSAVLGAVGGYMILWCVYWAFRLIAKKEGMGFGDFKLLAA
                     IGAWTGWQVLPVVIVISAGLGAVIGSIALALSKKGTDTRIPFGPYLALGGIAALLWGR
                     EAVTAWLGRFPA"
     gene            complement(1168..2397)
                     /locus_tag="VF309_08470"
     CDS             complement(1168..2397)
                     /locus_tag="VF309_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7466662.1"
                     /translation="MAAVAKKDTGKDVKLYNFKWSGKDKSGKTQTGEIRAAGDHIVLA
                     QLRRQGMQKISVKKVSSRGGKKISEKDLTIFTRQLAVMMKAGVPLLQAFDIVGRGHSN
                     PSVGKLLLDIKTDVETGSSLAQAFRKYPLYFDALFCNLVAAGEQAGILDTLLERLATY
                     QEKILAIKGKIKSALFYPISIIVVALVVTAVIMIFVIPVFKDLFKGFGAELPGPTLIV
                     MNISDWFVGNWWWVLPSMIGGIWFFFYTWKRSATMQHFMDRLILRVPVFGEVIRKACI
                     ARWCRTLSTMFAAGVPLVESLDSVAGASGNFVYYEATKKVQSEVSTGTGLTVAMQNAG
                     VFPSMVLQMCAIGEESGALDAMLSKVADFFEAEVDEAVESLSSLMEPMIMVVLGGLIG
                     GIVVAMYLPIFKMGQAI"
BASE COUNT          457 a          853 c          832 g          409 t
ORIGIN      
        1 gagcgcgccg tcggcgccga tgaaggccgc gccgaacgcg tcgcgaaccc cgtcgatggc
       61 ggcgccgccg gcgccggtga gggagcgcgc gatcgcatcg gtgtcgacca gcgtcgcgcc
      121 gagacccgcg aggcggtcgg cgacggtggt cttgcccgag ccgattccgc cggtcaggcc
      181 gatcgcgatc acgccgggaa gcggccgagc caggcggtaa cggcttcgcg gccccagagg
      241 agcgcggcga ttccgcccag cgcgaggtag ggaccgaacg ggatgcgggt gtcggtgccc
      301 ttcttcgaga gcgccagcgc gatcgagccg atcaccgcgc ccagtcccgc ggagatgacg
      361 atgacgacgg gaagcacctg ccagccggtc cacgcgccga tcgccgcgag cagcttgaag
      421 tcgccgaagc ccatgccctc cttcttcgcg atgaggcgaa aggcccagta gacgcaccag
      481 aggatcatgt acccgcccac cgccccgagc accgccgagc gcaggtccgt gtaagtcccc
      541 gcgacgttgg cgatgagccc cagccaaagg agcggcagcg tgaggtcgtc gggcagcagc
      601 tgcgtgtcga agtcgatgaa ggcgagcgcg agcatcaccc agacgtagac gagcgcgtag
      661 gcgagggccg tcgagtagcc gaagcgccac gccaccagcg ccgcgagcgc ggcgccgagg
      721 atctccacca tgggatagcg tgcgctgatc ggcgccgcgc accgtgcgca cttgccgcgc
      781 agcagcagcc agctcaccac ggggatgttc tgcagcgcgg tgatcggcgc cttgcactgc
      841 gggcaagccg agcggggcgt aaagagattg taggtctccc ggggcgtcgg ctcccggccg
      901 tcgagggacg cgcactcatc gcgccagtcg gcctccatca tcttcggaag gcggtggatc
      961 accacgttga ggagcgagcc gatgcacaag cccacgaggc caaccgcgac catgagcatc
     1021 gccggcgatt gccgcaggac ttcgaggaat tccatggcgg gtcaggcgcg aggcacgagg
     1081 cgcgagaaga acagggccgc accgaagtgc ggccctgttg cgcggctttt gcctcttgtc
     1141 tctatctcgt gcctcgtgcc tcgcgcctca gatcgcctgg cccatcttga agatcggcag
     1201 gtacatggcg accacgatgc cgccgatgag gccgcccagc accaccatga tcatgggctc
     1261 catcagcgac gagagcgact cgaccgcctc gtcgacttcc gcctcgaaga agtcggcgac
     1321 cttggagagc atcgcgtcga gcgcgcccga ttcctcgccg atcgcgcaca tctgcagcac
     1381 catgctcggg aagaccccgg cgttctgcat cgcgacggtg agcccggttc ccgtggagac
     1441 ttccgactgc accttcttcg tcgcctcgta gtagacgaag ttgcccgagg cgcccgccac
     1501 cgagtcgagc gactcgacca gcggcacgcc cgcggcgaac atcgtggaaa gggtgcggca
     1561 ccagcgcgcg atgcaagcct tgcggatcac ctcgccgaag accggaaccc tgaggatcag
     1621 ccggtccatg aagtgctgca tcgtcgccga gcgcttccac gtgtagaaga agaaccagat
     1681 gccgccgatc atcgacggaa ggacccacca ccagttgccg acgaaccagt ccgagatgtt
     1741 catcacgatc agcgtggggc cgggaagctc cgcgccgaat cccttgaaga ggtccttgaa
     1801 caccgggatc acgaaaatca tgatgaccgc ggtgacgacc agcgccacca cgatgatcga
     1861 gatcggatag aagagcgcgc tcttgatctt gcccttgatc gcgaggatct tctcctgata
     1921 ggtcgcgagc cgctcgagga gcgtgtcgag aataccagcc tgctcgcccg cggccacgag
     1981 gttgcagaac agggcgtcga aatagagcgg gtacttgcgg aaggcctgcg caagggacga
     2041 cccggtctcg acgtcggtct tgatgtcgag gagaagcttg cccacggaag ggttgctgtg
     2101 gccgcgcccc acgatgtcga aggcctgcag cagcggcacg cccgccttca tcatcaccgc
     2161 gagctggcgg gtgaagatcg tgaggtcctt ctcggagatc ttcttgccgc cgcgcgagct
     2221 caccttcttc acggagatct tctgcatccc ctggcggcgc agctgcgcca gcacgatgtg
     2281 gtcgcccgcc gcgcggatct cgcccgtctg ggtcttgccg gacttgtcct tgccgctcca
     2341 cttgaagttg tagagcttga cgtccttgcc ggtatctttc ttcgcgacgg ctgccatgtt
     2401 cgtctcccta ctcgttggtg acggcctcga cctcttcgag ggacgtcaga cccttcttca
     2461 ccttgatcag ccccgactgg cgcagatcct tgattccttc gcgcgacgcc tggtcggcca
     2521 ggtcgatcgc gtttccattc ttcatgatga t
//
LOCUS       DASVII010000655         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_209944,
            whole genome shotgun sequence.
ACCESSION   DASVII010000655 DASVII010000000
VERSION     DASVII010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209944"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF309_08475"
     CDS             complement(<1..468)
                     /locus_tag="VF309_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466663.1"
                     /translation="MSSPASALPLEERRALPAILSWRALTIFITASAVLGPLALIVYQ
                     SFLTAPFFDARKTIGIEAYEFVFADADFWKALLNSVLIATGMTLIAVPLGSLLAFVME
                     RTDLPGKRWFEPLILVPSFVSPMVLSFGFVVAAGPVGFYTVWAQDLFGVAPWGI"
     gene            complement(468..1529)
                     /locus_tag="VF309_08480"
     CDS             complement(468..1529)
                     /locus_tag="VF309_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466664.1"
                     /translation="MVSATALAQVPAGYPADYAQTVAAAKKEGKVVIYSALDTKAAQP
                     LIKDFNALYPDIKVEYNDMNSTELYNRFIAEVASGQGSADIMWSSAMDLQVKLVDEGH
                     AATYASPEVPKLPKWAVYKNQAYGTTYEPAVFIYNKRLMTGDEIPTDHASFAKVMAAK
                     TDKLKGKVTTYDIEKSGVGFMFVVLDSKYFPGMADIEKGFGATGYKVYSSTGNMLEKV
                     SSGEHLLGYNVLGSYALVRSKKDPNLGVVLPKDYTLVLSRVMFIGKAAKNPNAAKLWT
                     DYVLSIRGQKMIGGDVELFSMREDVDAEYTAAKLNKQLAGAVKPIPVAAEITEYLDQK
                     KRLDFLGKWKAAVAAGGGK"
     gene            1528..>2079
                     /locus_tag="VF309_08485"
     CDS             1528..>2079
                     /locus_tag="VF309_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466665.1"
                     /translation="MAAAAQPLENLEPVHARQAEIEDHEVVLLREQDVVGLDPVAHAI
                     DREAAVAQRFLQPGGQRPVVFRKKDSQGCSRGDSTGKVVAQMLQWSARERSLKGRGGK
                     NAAQTRTNCPLEKRMKKVSAVMALAVMSLEVLAQAPAGYPADYAQTIAAARKEGKVVI
                     YSALDTKAAQPLIKDFNVVYPDVK"
BASE COUNT          374 a          650 c          686 g          369 t
ORIGIN      
        1 gatcccccag ggcgcgacgc cgaagaggtc ctgcgcccac accgtgtaga agccgaccgg
       61 tcccgcggcc accacgaagc cgaacgacag caccatcggc gagacgaagc tcggcaccag
      121 gatgagcggc tcgaaccagc gcttgcccgg caggtcggtg cgctccatca cgaaggcgag
      181 gagcgagccc agcgggaccg cgatcagcgt catgccggtc gcgatgagga cggaattgag
      241 cagcgccttc cagaagtcgg cgtcggcgaa gacgaactcg taggcttcga tcccgatcgt
      301 cttcctcgca tcgaagaagg gcgcggtgag gaagctctgg tagacgatga gggcgagcgg
      361 cccgaggacg gcgctcgccg tgatgaagat ggtgagcgcg cgccacgaaa gaatcgccgg
      421 cagggcgcgt cgctcctcga gaggcagcgc gcttgccggc gacgacatta cttgcctccg
      481 cccgcggcga ccgccgcctt ccacttgccg aggaagtcga ggcgcttctt ctggtcgaga
      541 tactcggtga tctcggccgc gacgggaatc ggcttcacgg cgccggcgag ctgcttgttc
      601 agcttggccg cggtgtactc ggcgtccacg tcctcacgca tcgagaagag ctcgacgtcc
      661 ccgccgatca tcttctgtcc gcgaatggac agcacgtagt cggtccagag cttggcggcg
      721 ttggggttct tggccgcctt gccgatgaac atcacccgcg agagcaccag cgtgtaatcc
      781 ttgggcagaa ccacgcccag gttggggtcc ttcttggagc gcaccagcgc atacgacccc
      841 agcacgttgt agcccagcag gtgctcgccg gaggagactt tctccagcat gttgccggtg
      901 ctcgagtaga ccttgtagcc ggtcgcgccg aagcccttct cgatgtcggc catgcccggg
      961 aagtacttcg agtcgagcac cacgaacatg aagcccacgc cgctcttctc gatgtcgtag
     1021 gtggtgacct tgcccttcag cttgtcggtc ttggcggcca tcaccttcgc gaacgaggca
     1081 tggtcggtcg ggatctcgtc gccggtcatg aggcgcttgt tgtagatgaa caccgccggc
     1141 tcgtaggtgg tgccgtaggc ctggttcttg tacaccgccc acttgggcag cttgggcact
     1201 tccggcgagg cgtacgtcgc ggcgtggccc tcgtccacca gcttcacctg gaggtccatg
     1261 gccgagctcc acatgatgtc cgccgagccc tgccccgagg ccacttccgc gatgaagcgg
     1321 ttgtagagct cggtggagtt catgtcgttg tactcgacct tgatgtcggg atacagcgcg
     1381 ttgaaatcct tgatgagcgg ctgcgccgcc tttgtgtcga gcgcgctgta gatcacgacc
     1441 ttgccttcct tcttggcggc ggcgacggtc tgcgcgtaat cggcggggta gcccgcgggg
     1501 acctgggcga gggcggtggc ggaaaccatg gccgcggcgg cgcagccgct tgagaatctc
     1561 gagcccgtcc atgcgcggca ggccgagatc gaggatcacg aggtcgtact cctgcgtgag
     1621 caggacgtgg tcggcctcga ccccgtcgct catgcaatcg accgcgaagc cgccgtggcg
     1681 caacgctttc tccagccagg cggccagcgt cctgttgtct tccgcaagaa ggattcgcaa
     1741 gggtgctccc gcggcgactc tacaggaaaa gttgttgcgc aaatgttgca gtggagcgcg
     1801 cgtgaaagga gtttgaaagg tcgcgggggc aagaatgcgg cccaaacaag aacgaattgt
     1861 cccttggaga aaagaatgaa gaaggtttcc gccgtcatgg cgctcgccgt gatgtcgctc
     1921 gaagtgctcg cccaggcgcc cgccggctat cccgccgact acgcacagac cattgccgcc
     1981 gcgaggaagg aaggcaaggt cgtgatctac agcgcgctcg acaccaaggc cgcgcagccg
     2041 ttgatcaagg acttcaacgt cgtgtatccc gacgtgaag
//
LOCUS       DASVII010000656         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_354264,
            whole genome shotgun sequence.
ACCESSION   DASVII010000656 DASVII010000000
VERSION     DASVII010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354264"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VF309_08490"
     CDS             complement(<1..883)
                     /locus_tag="VF309_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7466666.1"
                     /translation="MRRSNALGLLVLAAASVLGGCSLAPALDIPDVPAAPAYKEESPW
                     ARAAPADELPREAWWTLYGDAELDALQSRLVAGSPDLAAALARYQQARAFTDQLRSGF
                     YPSITASANAQRLRQSQEKPLRVLGPTSPDNYSSVTLGAEVDYEFDLWGRIRNQVAAG
                     VATEAAAQADLENARLSLQAQLADDYIALRGLDREVALFDATVTAYGKALELTRSRHD
                     GGIASGLDVSRAETQLESVRSQAAQQVAQRAVLEHAIAALIGESPSRFAIEPRLAEIN
                     LPAIPPGVPSTLLQRRPD"
     gene            complement(870..2033)
                     /locus_tag="VF309_08495"
     CDS             complement(870..2033)
                     /locus_tag="VF309_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7466667.1"
                     /translation="MPHDSTPPVVSRRKLRIAGIVLLAIAAGVAAFGIATRAADDKTL
                     RERAEAQSVPTVAVIAPGNSGDAGTLELPGRLEAYSRASIYARVSGYLKSWKADIGTP
                     VKAGQLLAEIETPDLDQQLLQAKADLASAQANASLAGTTAKRWQSMLGSDSVSRQEAD
                     EKTGDAATKEALVNAAQANVDRVQALKGYTRIVAPFDGIVTSRNTDVGALINAGSGTG
                     PELFSVSDVRKLRVYVSVPQSYASIVKVGAKAKLTVPERLGKSYSAKVESSSGAVNVA
                     SGSMLIQLAVDNAAGELLPGSFANVSLEQPRTMGGLSIPPSALLFDKSGLRVATVGAD
                     DKVLLKRVTISRDLGKVIELGSGIAPGDRVIETPPDGIADGDLVRIAKADEKK"
     gene            complement(2023..>3692)
                     /locus_tag="VF309_08500"
     CDS             complement(2023..>3692)
                     /locus_tag="VF309_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7466668.1"
                     /translation="PLVRFQRGFEARFERVRGAYRELLSMALAGRSVFVIGFLAVVVL
                     SFALVPFLGRNFFPSVDGGQILMHARVPVGTRVEETASRFAAIEAAIRRVIPAEEIAT
                     LVDNIGLPPSSINLTYNNTGVVGTQDGDVQIALSRDHHPTADYVRELRERLSREFPDT
                     VFSFPPADIVSQILNFGAPAPIDLQVRGANLDANFDYANNLLKAIRRIPGVADARIQQ
                     SRRSPVFDVNMNRTRAGELGITARDVTNSLVVNLAGSSQVAPTFWLNPANGISYAIVM
                     QTPQYKLDSLPALANLPINGANPGPSQVLGGLATFNRSAANAVVTQYDIAPMVQINAT
                     TQDRDLGAVATDIRRVIAETAGQVPKGSSVALLGQVKTMDNAFSGLLFGLLGAIVLIY
                     FLIVINFQSWSDPFVIITALPAALAGIVWMLFATHTTLSVPALTGAIMCMGVATANSV
                     LVISFARERLDVLGDSAAAALDAGFVRFRPVLMTALAMIIGMAPMALGLGEGGEQNAP
                     LGRAVIGGLVFATIATLMFVPVVFSIVHARHGRKPAPGAALGDLHAA"
BASE COUNT          570 a         1232 c         1324 g          566 t
ORIGIN      
        1 tgtccgggcg gcgctgcagc agcgtcgagg gcacgcccgg agggatcgcc ggaaggttga
       61 tctccgccag ccggggctcg atcgcgaatc gcgaaggcga ctcgccgatg agcgccgcga
      121 tcgcatgctc gagcaccgcg cgctgcgcca cctgctgcgc cgcttgcgag cggacgctct
      181 cgagctgcgt ttccgcgcgg gagacgtcga ggccggaagc gatgccgccg tcgtgccggg
      241 agcgggtgag ctcgagcgcc ttgccgtagg ccgtgacggt cgcgtcgaag agcgcgacct
      301 cgcgatcgag accgcgaagc gcgatgtagt cgtcggcaag ctgcgcctgc aggctgaggc
      361 gcgcgttctc gagatcggcc tgcgcggcgg cctcggtggc cacgccggca gccacctggt
      421 tgcggatcct tccccacagg tcgaactcgt agtccacctc ggcgcccagc gtgacggagc
      481 tgtagttgtc gggcgacgtg gggccgagca ctcgcagcgg cttttcctgc gactggcgca
      541 ggcgctgcgc gttcgccgag gccgtgatgg acggatagaa gccggagcga agctggtcgg
      601 tgaatgccct tgcctgctgg tatcgcgcga gcgccgctgc gaggtccggg ctccccgcga
      661 cgaggcgcga ctgcagcgcg tcgagctccg cgtcgccgta cagcgtccac cacgcctcgc
      721 gcggaagctc gtcggcggga gcagcgcgcg cccagggcga ctcttccttg tacgcgggcg
      781 ccgcgggaac atcgggaata tcgagggccg gcgcgagcga gcatcccccc agcacgctcg
      841 ctgcggcgag gaccagcagt cccagcgcgt tacttcttct catcggcctt ggcgatccgc
      901 accaggtcgc cgtcggcgat gccatcgggc ggggtctcta tgacgcgatc gcccggggcg
      961 atgccagagc ccagctcgat caccttgccg agatcgcgcg agatggtcac ccgcttgagg
     1021 agaaccttgt cgtccgcgcc caccgtggcg acgcgcaatc ccgacttgtc gaacaggagc
     1081 gcgctcggcg ggatgctcaa tccgcccatg gtgcgcggct gctcgaggct cacgttcgcg
     1141 aagcttccgg gcaggagctc gcccgcggcg ttgtccacgg cgagctggat caacatgctt
     1201 ccggaggcga cgttcaccgc gcccgaagag gattccacct tcgcggagta gctcttgccc
     1261 aggcgctcgg gaaccgtgag cttcgctttc gcgcccacct tcacgatcga cgcgtagctc
     1321 tgcggcacgc tcacgtagac gcgaagcttg cgcacgtcgg aaacgctgaa gagctcgggg
     1381 cccgttccgc tgccggcgtt gatgagcgcg cccacatcgg tgttgcgcga agtgacgatg
     1441 ccgtcgaagg gcgcgacgat ccgcgtgtag cccttcaacg cctgcacgcg atcgacgttg
     1501 gcctgcgcgg cgttcaccag cgcctccttg gtcgcggcgt cgcccgtctt ctcgtcggcc
     1561 tcctggcgcg agaccgaatc ggagccgagc atcgattgcc agcgcttcgc cgtcgttccc
     1621 gcgagcgacg cgttggcttg cgcgctcgcg aggtccgcct tcgcctgcag cagctgctgg
     1681 tcgagatccg gcgtctcgat ttccgcgagg agctgtcccg ccttcaccgg cgttccgatg
     1741 tcggccttcc agctcttcag gtagccgctc acccgcgcgt agatcgacgc gcgcgaatac
     1801 gcctcgagcc ggccgggaag ctcgagcgtg ccggcgtcgc ccgagttgcc gggcgcgatc
     1861 acggcgaccg tcggcaccga ctgcgcctcc gcgcgctcgc gaagcgtctt gtcgtccgcc
     1921 gcgcgcgtgg cgatgccgaa ggccgcgacg ccggctgcga tcgcgaggag gacgatcccc
     1981 gcgatgcgca gcttccggcg cgacacgacg ggaggggtgg aatcatgcgg catgaagatc
     2041 tccaagggcg gcgccgggcg cgggcttgcg gccgtggcgc gcgtgcacga tgctgaacac
     2101 gacgggcacg aacatgaggg tggcgatggt ggcgaagacg aggccgccga tgaccgcgcg
     2161 gcccagcggc gcgttctgct cgccgccctc ccccaggccc aacgccatcg gcgccatgcc
     2221 gatgatcatc gcgagcgccg tcatgagaac ggggcggaag cgcacgaatc ccgcgtcgag
     2281 cgcggcggcg gccgagtcgc cgagcacgtc gaggcgctcg cgcgcgaagc tgatgacgag
     2341 gacgctattg gcggtcgcga cccccatgca catgatcgcg cccgtgaggg ccgggaccga
     2401 cagcgtcgtg tgagtcgcga aaagcatcca cacgatgccc gcgagcgcgg cgggcagcgc
     2461 ggtgatgatg acgaaaggat cgctccacga ctggaagttg atgacgatga ggaagtagat
     2521 gagcacgatc gcgcccagga ggccgaagag gagccccgag aacgcgttgt ccatcgtctt
     2581 cacctggccc aggagcgcga cgctggagcc cttcggcacc tggcccgccg tctccgcgat
     2641 gacgcgccgg atgtccgtcg cgaccgcgcc caggtcgcga tcctgcgtcg tcgcgttgat
     2701 ctgcaccatc ggcgcgatgt cgtactgggt cacgacggcg ttggccgcgc tgcggttgaa
     2761 ggtggcgagg cccccgagga cctgcgacgg accggggttg gcgccgttga tcggcaggtt
     2821 cgccagggcc gggagcgagt cgagcttgta ctgcggagtc tgcatcacga tcgcgtagga
     2881 aatcccgttt gccgggttga gccagaaggt cggcgcgacc tggctgcttc ccgcgaggtt
     2941 cacgacgagg ctgttggtca cgtcgcgcgc ggtgatgccg agctcgcccg cccgcgtgcg
     3001 gttcatgttc acgtcgaaga cggggctgcg gcgcgattgc tggatgcgcg cgtcggcgac
     3061 cccgggaatg cggcggatcg ccttgagcaa gttgttggca tagtcgaagt tggcgtccag
     3121 gttcgctccg cggacctgca agtcgatggg cgcgggagcg ccgaaattga ggatctggct
     3181 cacgatgtcg gccggcggga acgagaacac cgtgtcggga aactcccgcg acaggcgctc
     3241 gcgcagctcg cgcacgtaat cggcggtcgg atgatggtcg cgggagagcg cgatctgaac
     3301 gtcgccgtcc tgcgtgccca ccacgcccgt attgttgtag gtgaggttga tgctgctcgg
     3361 gggaaggccg atgttgtcca cgagtgttgc gatttcctcc gcgggaatga cgcggcggat
     3421 cgcggcctcg atggcggcga agcgcgacgc cgtctcctcg acccgcgtgc cgacgggaac
     3481 cctcgcgtgc atgaggatct gcccgccgtc cacggacgga aagaagttcc ggccgaggaa
     3541 gggcaccagc gcgaacgaga ggaccaccac cgcgaggaag ccgatcacga agacgctcct
     3601 cccggccagc gccatggaaa gcagctcccg gtaggcgccg cgcacgcgct cgaagcgcgc
     3661 ctcgaatccg cgctggaaac gcaccagggg at
//
LOCUS       DASVII010000657         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_605984,
            whole genome shotgun sequence.
ACCESSION   DASVII010000657 DASVII010000000
VERSION     DASVII010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605984"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..957)
                     /locus_tag="VF309_08505"
     CDS             complement(349..957)
                     /locus_tag="VF309_08505"
                     /inference="COORDINATES: protein motif:HMM:NF017494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7466669.1"
                     /translation="MTHHVLRMPVTEAQARALKVDDTVTIEGALFGIRDANQIALFDK
                     GRTTRFDMRGHAAIHTAPNVRKVPPSPEHPAGYAPICVGTTTSARMERFTRPLMEKLG
                     VRILIGKGGLGAGSLAAFAELGGAYLAIIGGTAALETTWIEAIEDVDLDDLNPESLWK
                     FRVRGFGPLLVAMDSHGGSLYDAVDRKARARRADTLARLGVK"
     gene            complement(968..1840)
                     /locus_tag="VF309_08510"
     CDS             complement(968..1840)
                     /locus_tag="VF309_08510"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX7466670.1"
                     /translation="MKIDPAQVEEVARELYVRALKILPPDIKRGFARLDATETDATAK
                     KVLGTMSTNIRVAEETENLLCQDTGIPIYNVTIGRGVEIAGTELRDAIRRGCERATRE
                     HPLRSSVVHPITRRNEHTSCGAGVPPIHVDFCEEERLVIEMIPKGSGSENNSWLKMAV
                     PADGVNAIKTFVIDCVLEAGGKTCPPTIVGVGIGGTSDLCMALAKRAATRPLGTSCAD
                     PEGAALEASLTEAVNGLGVGPQGLGGDSTAFAVHVEIAATHITMNPVAVNMQCHSARR
                     ASASFTRAGVEYGF"
     gene            complement(1840..>2195)
                     /locus_tag="VF309_08515"
     CDS             complement(1840..>2195)
                     /locus_tag="VF309_08515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7466671.1"
                     /translation="LTAAINFIQQGKLRALAVMSRERSAQLPDTPTAAEFLPGFENAG
                     WFALMAPTGTPREVIDRIQGDTVKILLSEDFRAKLAQQGMTPMATTPAELGALIRDDS
                     ARWAKVIRERGLQAN"
BASE COUNT          319 a          747 c          748 g          381 t
ORIGIN      
        1 cttcaggacc tcgatcgccc tgcgcgcgag gccggtgacg agcgcatagc cgatgacgat
       61 gtgcagcacc acgaccatgg cgatgctcgg caggtgcttc accgggtttc gctcacgctc
      121 tgcgtagttc actctgcgac ctccctcgac tgcgcgaaga ccaccgttcg gggtcctgcc
      181 tgttgcgggc atgccctcgt gaggcacgcc tgaaattggg ggcggcggcg aggggcgaac
      241 ccctggccgg atggaatccc cttggccgat tgcaaaacgc gcgagtttac cttattcctt
      301 gtggaaaagg acagcggctc ataccgcgta acacgggtcc aagggtgctc atttgacacc
      361 caatcgggcc agggtgtccg cccggcgcgc ccgcgccttg cgatccacgg cgtcgtaaag
      421 gcttccccca tggctgtcca tcgcgaccag cagggggccg aagcccctca cgcggaattt
      481 ccacagggat tcggggttca ggtcgtccag gtccacgtcc tcgatcgcct cgatccacgt
      541 cgtctcgagg gccgccgtgc ccccgatgat cgcgaggtaa gccccgccca gctcggcgaa
      601 ggccgcgagg gagccggcac cgagccctcc cttgccgatg agaatgcgca cgcccagctt
      661 ctccatgagg ggccgcgtga agcgctccat gcgcgccgag gtggtggtgc cgacgcagat
      721 cggggcatag cccgccggat gctcggggga tgggggcacc ttcctcacgt tgggcgcggt
      781 gtggatcgcg gcgtggccgc gcatgtcgaa gcgggtcgtg cgccccttgt cgaagagcgc
      841 gatctggttg gcgtcgcgaa tgccgaagag cgcgccctcg atggtgacgg tgtcgtccac
      901 cttcagcgcg cgcgcctgcg cctcggtcac gggcatccgc aggacgtggt gggtcatcgc
      961 atggcgccta gaagccgtac tcgacgccgg cccgggtgaa cgaggccgag gcgcgccgcg
     1021 ccgagtggca ctgcatgttc accgcgacgg ggttcatcgt gatgtgcgtc gccgcgatct
     1081 cgacgtgcac cgcgaacgcg gtggagtccc cgcccaggcc ctggggaccc acgccgagcc
     1141 cgttcaccgc ctcggtgagg ctcgcctcga gcgcggcgcc ttcgggatcg gcgcacgaag
     1201 tcccgagcgg gcgggtcgcc gcgcgcttgg cgagagccat gcacaggtcc gaggtcccgc
     1261 cgatgccgac accgacgatc gtgggggggc aggtcttgcc gcccgcctcg agcacgcagt
     1321 cgatcacgaa ggtcttgatg gcgttcacgc cgtcggcggg aaccgccatc ttgagccacg
     1381 agttgttctc cgatccgctg cccttgggga tcatctcgat caccaggcgc tcctcctcgc
     1441 agaaatcgac gtggatcggc ggcacgcccg cgccgcacga ggtgtgctcg ttcctgcgcg
     1501 taatgggatg gaccacggag gagcgcagcg gatgctcgcg cgtcgcgcgc tcgcagccgc
     1561 ggcgaatggc gtcacgcagc tccgtgccgg cgatctcgac gccgcgcccg atcgtcacgt
     1621 tgtagatcgg gatgccggtg tcctggcaga ggaggttttc cgtctcctcc gcgacgcgga
     1681 tgttggtgct catcgtcccc agcaccttct tcgccgtggc gtcggtctcg gttgcgtcca
     1741 ggcgcgcgaa accccgcttg atgtccggcg gcaatatctt caacgcccga acgtagagct
     1801 cgcgcgcgac ctcctcgact tgcgccggat cgatcttcat cagttggcct gcaggcccct
     1861 ttcgcggatc accttcgccc accgcgcgga atcgtcgcgg atgagggcgc ccagctccgc
     1921 gggcgtggtc gccatcggcg tcatgccctg ctgcgcgagc ttggcgcgga agtcttccga
     1981 aaggaggatc ttcaccgtgt cgccttggat gcggtcgatc acctcgcgcg gcgtcccggt
     2041 gggcgccatc aaggcgaacc atcccgcgtt ctcgaatccc ggcaggaatt cggcggcggt
     2101 cggcgtgtcg gggagctgcg cactgcgctc ccggctcatg accgccagcg cgcgcagttt
     2161 tccctgctgg atgaagttga tcgcggcggt gagat
//
LOCUS       DASVII010000658         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_498164,
            whole genome shotgun sequence.
ACCESSION   DASVII010000658 DASVII010000000
VERSION     DASVII010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498164"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1271)
                     /locus_tag="VF309_08520"
     CDS             complement(198..1271)
                     /locus_tag="VF309_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7466672.1"
                     /translation="MTDSTRSLIVSSFAAGVAAALLAWPVGAQVKPGATAAEVASYGG
                     PDRVQKLIEGAKKEGELSIYTSAQSDDMGVLVAAFEKKYGVKVSVWRSSSEKVLQRAV
                     TEARGNRFTMDIAETNGPELEALHREKILEAVKSPYLADLMPQAIMPHGEWVGTRLNV
                     FIQAYNTSLVKKEDLPRTWEELAGPKWKGKLGIEQEDVDWLAGEYAELGEARAAKVFK
                     DIVANNGISVRKGHTLLTQLVVSGEIPLALTVYNYKAEQLKNKGAPIDWFTIGPAIAR
                     PNGIGVARKAPHPHAAVLFYDFEISPEGQKILAGRDFVPTSRKVDTPLNKVPMKFVDS
                     RVALDEFQKWGKLYADLFTKGVR"
     gene            complement(1361..>1712)
                     /locus_tag="VF309_08525"
     CDS             complement(1361..>1712)
                     /locus_tag="VF309_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466673.1"
                     /translation="FDENATVTGRFLSYKNNAYQVAWDARWNGQWRTALEYIKSMKGT
                     CSRVNAACNTDGLDGSQLQFGVAYYFSKRTYVFAMAALLRNGFSAQYNNSASQTPSVG
                     EDLDQYAVGLSHNF"
BASE COUNT          254 a          565 c          551 g          342 t
ORIGIN      
        1 ttgcccttga tgacgcgtgg catcgagcgc aggcgcttca gctcgtcgga gaggctgtac
       61 gcgccctgga tgccgcgaac gaagacgcgg tccttcttgc tgctgtagtg cgattcttta
      121 cccatttgcg cctcccttcg agggtgcttc gacggtgctt cgcaaccacg ctgccggtgc
      181 ttcgcagcca cgacccatca acgcactccc ttcgtgaaaa ggtccgcgta gagcttgccc
      241 catttctgga actcgtcgag cgccacgcgc gagtcgacga acttcatcgg gaccttgttc
      301 agcggggtgt cgaccttgcg gctggtaggg acgaaatcgc ggccggcgag gatcttctgc
      361 ccctcgggac tgatctcgaa atcgtagaag aggaccgcgg cgtggggatg cggggccttc
      421 ctcgcgacgc cgatgccgtt cgggcgcgcg atcgccgggc cgatggtgaa ccagtcgatc
      481 ggggcgccct tgttcttcag ctgctcggcc ttgtagttgt agaccgtgag cgcgagggga
      541 atctcgcccg acaccacgag ctgcgtaaga agcgtgtgcc ccttgcgcac cgatatgccg
      601 ttgttggcga cgatgtcctt gaacaccttg gccgcgcgcg cctctcccag ctccgcgtac
      661 tcgcccgcga gccagtcgac gtcctcctgc tcgatgccga gcttgccttt ccacttgggc
      721 cccgcgagct cttcccatgt cctgggcagg tcttccttct tcacgaggct cgtgttgtag
      781 gcctggatga agacgttcaa gcgcgttccg acccattcgc cgtggggcat gatcgcctgg
      841 ggcatgagat cggcgagata gggcgacttc accgcctcga ggatcttctc gcggtgcagc
      901 gcctcgagct cgggcccgtt ggtctcggcg atgtccatgg tgaagcggtt gccgcgcgcc
      961 tcggtgaccg cgcgctgcag caccttctcg ctcgaggagc gccacacgct caccttgacg
     1021 ccgtacttct tctcgaacgc ggcgaccagc acgcccatgt cgtcgctctg cgcggaggta
     1081 tagatgctga gctcgccttc cttcttcgcg ccctcgatga gcttctgcac gcggtcgggc
     1141 cccccatagg acgcgacctc ggccgcggtg gcgccgggct tcacctgcgc ccccacgggc
     1201 cacgcgagca acgccgcggc gacgccggcg gcgaaggagg acacgatgag actgcgcgtg
     1261 ctgtcggtca ttcgatgcct caaaaagaga cgggcgcctg ggcgcccgcc gattggttca
     1321 tgttcccgga gcccgccagg gcgcccgcat catcgcagcc tcagaagttg tggctgagac
     1381 cgaccgcgta ctggtcgaga tcctcgccca cgctcggcgt ctgcgaggcg ctgttgttgt
     1441 attgcgccga gaatccgttg cgcagcaggg ctgccatcgc aaacacgtag gtgcgcttgg
     1501 agaagtagta cgcgacgccg aactgcagct gcgagccgtc gaggccgtcg gtgttgcagg
     1561 cggcgttcac gcgcgagcag gtgcccttca tcgacttgat gtattccagc gccgtgcgcc
     1621 attggccgtt ccagcgcgcg tcccatgcga cctggtacgc gttgttcttg tagctgagga
     1681 agcggccggt gaccgtcgcg ttctcgtcga ac
//
LOCUS       DASVII010000659         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_282673,
            whole genome shotgun sequence.
ACCESSION   DASVII010000659 DASVII010000000
VERSION     DASVII010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282673"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VF309_08530"
     CDS             <1..477
                     /locus_tag="VF309_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HEX7466674.1"
                     /translation="VTPDLARKLGLAGLAGRSSGQAFDLRADFQTPPYDELAVAKVVF
                     AAGDVATRVHARFDEVHESLRLIREVLARAPQGSIRGDLHAPRGGFGVGWIEGWRGEV
                     LVAIEAEADGRIRRAHAHDPSWQNWPLLEHAVIGNIVPDFPLINKSFNLSYSGHDC"
     gene            477..992
                     /gene="nuoB"
                     /locus_tag="VF309_08535"
     CDS             477..992
                     /gene="nuoB"
                     /locus_tag="VF309_08535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX7466675.1"
                     /translation="MIPILLKILKTGVVTEPPGEARDEWRARAAQIQDEVLKLLGRAL
                     VIRHVDAGSCNGCELELHALNNPYYNIEGLGIRFAASPRHADMLLVTGPVSRHMENAL
                     RIAYDAMPDPKLVVAVGDCGRDGGIWSGSYATCGGVASVIPVDVVVPGCPPPPAAVLQ
                     GILTAIATKAR"
     gene            1097..1516
                     /locus_tag="VF309_08540"
     CDS             1097..1516
                     /locus_tag="VF309_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466676.1"
                     /translation="MKRNWILATIPALAIVSFAAMPLQAQVAPLDGRAFVAEAGENGK
                     AADEKNDVITFAGGKFHSSACDQYGFGKGDYRASAAGDAVSFEAETQSDKDGRLVWKG
                     TVRGSDIEGTFVHYRKGWFLNPNPEPVQHWFKGRVKA"
     gene            complement(1550..>1910)
                     /locus_tag="VF309_08545"
     CDS             complement(1550..>1910)
                     /locus_tag="VF309_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7466677.1"
                     /translation="APAYAIPRLLARNGLTLGAIDLWEIHEAFAAQVLCNVAAIESED
                     FLREEVRIDARLGKFPWERLNPNGGSIAIGHPFGATGARILSQAVKELAAMGPGKRAV
                     VSICADGGVGTVALLAN"
BASE COUNT          295 a          675 c          658 g          282 t
ORIGIN      
        1 gtgaccccgg acctcgcgcg caagctcggc ctcgccgggc tcgccggccg ctcgagcggc
       61 caggcgttcg accttcgcgc cgatttccag acgccgccct atgacgagct cgcggtggcg
      121 aaggtcgtgt tcgccgcggg cgacgtcgcg acgcgcgtgc acgcgcgctt cgacgaggtg
      181 cacgagtcgc tgcgcctcat ccgcgaggtg ctcgcgcgcg cgccccaggg atcgatccgc
      241 ggcgacctgc acgcgccgcg aggcgggttc ggcgtgggct ggatcgaagg ctggcgcggc
      301 gaggtgctgg tcgcgatcga ggccgaggcc gacgggcgca tccgccgcgc ccatgcgcac
      361 gatccctcgt ggcagaactg gccgctcctc gagcacgcgg tgatcggcaa catcgttccc
      421 gacttcccgc tcatcaacaa gagcttcaac ctcagctact ccgggcacga ctgctgatga
      481 ttcccatcct gctgaagatc ctgaagaccg gagtcgtcac cgagcccccg ggcgaggcgc
      541 gggacgagtg gcgcgcgcgc gccgcgcaga tccaggacga ggtgctcaag ctcctcgggc
      601 gcgcgctcgt gatccggcac gtcgacgcgg gatcgtgcaa cggctgcgag ctcgagctgc
      661 acgcgctcaa caacccgtac tacaacatcg agggactcgg catccgcttc gcggcgagcc
      721 cgaggcacgc cgacatgctg ctggtgacgg gcccggtgtc gaggcacatg gagaacgcgc
      781 tgcgcattgc ctacgacgcg atgcccgatc cgaagctcgt cgtcgcggtg ggcgattgcg
      841 ggcgcgacgg cggcatctgg agcggcagct acgcgacctg cggcggcgtc gcgagcgtga
      901 ttcccgtcga cgtcgtcgtt cccggctgcc cgccgccgcc cgcggccgtg ctccagggga
      961 ttctcacggc gatagccacg aaggctcgct gacgccggcc gctgcggctt gcctacgcaa
     1021 agttgcgtat ttcccgatcg ccgcgccgag cctagtgttt gctccatcgt catccgacgc
     1081 tgccgaaagg acccccatga aacgcaactg gattctggcc acgattcccg ccctcgccat
     1141 cgtgtcgttc gccgcgatgc cccttcaggc ccaggtcgcc cctctcgacg gccgcgcgtt
     1201 cgtcgccgaa gcgggcgaga acggcaaggc cgccgacgag aagaacgacg tcatcacctt
     1261 cgcgggcggc aagttccatt cgagcgcctg cgaccaatac ggattcggca agggcgacta
     1321 ccgggcgagc gccgcgggcg acgcggtgag cttcgaggcg gagacccaga gcgacaagga
     1381 cgggcgcctc gtttggaagg gcacggtgcg cggctccgac atcgagggca cgttcgtgca
     1441 ctaccgcaag ggctggttcc tcaatcccaa tccggagccg gtgcagcatt ggttcaaggg
     1501 gcgcgtgaag gcctgacgcg ctgcgcgcgc ctcgcgggac gcggggccat cagttggcaa
     1561 gcagcgccac ggtgccgact ccgccatcgg cgcagatcga caccacggcg cgcttgccgg
     1621 gccccatcgc ggcgagctcc ttcaccgcct gggaaaggat gcgcgcgccg gtcgcgccga
     1681 acgggtgccc gatggcgatg ctgccgccgt tcggattcag gcgctcccac gggaatttcc
     1741 cgagacgcgc gtcgatgcgc acttcttcgc gcaggaagtc ctcgctctcg atcgccgcga
     1801 cattgcacag cacctgcgcc gcgaacgctt catggatctc ccagaggtcg atcgcgccga
     1861 gcgtgaggcc gttgcgcgcg agcaggcgcg gaatcgcgta ggcgggcgcc
//
LOCUS       DASVII010000660         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_31356, whole
            genome shotgun sequence.
ACCESSION   DASVII010000660 DASVII010000000
VERSION     DASVII010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31356"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VF309_08550"
     CDS             complement(<1..444)
                     /locus_tag="VF309_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7466678.1"
                     /translation="MPSRADHQEIVISTSFESLGLLPELLRAVAEQGYTEPTPIQSQA
                     IPIVLSRRDLLGAAQTGTGKTAGFTLPLLQRLAPMATKSFSPALHPVRCLILTPTREL
                     AIQVHESVKGYGKHIPLRSFVVYGGVNINPQIDEMKRGVEILVATP"
     gene            687..1889
                     /locus_tag="VF309_08555"
     CDS             687..1889
                     /locus_tag="VF309_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7466679.1"
                     /translation="MKRIPWLALVAAAGIGTATAQEPIAIGVSIAQSPPGSVVQGTQV
                     KDGVEIMKDMINAQGGVLGRPIKLIYEDNQGVPEKGRAATEKLITKDKVVAVTGGHQS
                     SVCLAEIEVAHRYETPYVNTNCWSDDVRKKGYAEVFNPSNYNTRVSTAMAETIAAMGV
                     KRVVAFAENTDYGIGQAKLMAEFIKAKAPNVQFKYETLDRAGKDFTPAVVALRADPPD
                     MVVNIMLPPAAYILMNQLYEQGVAPSAKTWSYDGAGIADYPDFWQNVKDAGKYMVSFG
                     LYHPMMPMPKVGKDVAAEYTKRTKNEPNRLIFQAADSLLLIADAIKQAKSADPKAITK
                     ALQTMKYDGVRGPFQFSHEPGYAFQQWVDIPYVNYQLTDVNQPVSKSNLIQGPGQPLA
                     VSKLQKPK"
     gene            1902..>3201
                     /locus_tag="VF309_08560"
     CDS             1902..>3201
                     /locus_tag="VF309_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7466680.1"
                     /translation="MRDTLIGFADWQGYGHGERSESWLQRLELNRPEIEAFRGARVPD
                     APPYVACAAELLPAEGVAAPDVKRPADPAAALARARAAARLNAFTHLPDGVEARGVGL
                     LAGMPIVVKDLMHVAGMPLSGGGKVMGRPMATRDAEIVARLRRAGAVVVGLANLHEFA
                     YGITSDNPHFGRVVNPVAPTRIPGGSSGGSAAAVAAGIVRFAVGTDTAGSIRVPAACC
                     GVVGFKPSYDALPRGGVIDLACSLDHVGPMTFDVEDCALMFAAMLDLQAAPRWVRKDL
                     VGVTAARLGGYFAEPLDREVAMAYDVAMRALARDGARCIDTSVEGVALAPAIQLNTIA
                     PEATAYHANLLASRGAEYGEDVRVRLEMGLFLPGHWYAKAQRMRTELVDRIEAAFGEA
                     DVLVCPTMRAPAPPVGALRIEIGRQAFALHTAVTNLTLPFN"
BASE COUNT          548 a         1055 c         1098 g          500 t
ORIGIN      
        1 tggagtcgcg acgaggatct cgacgccgcg cttcatctcg tcgatctgcg gattgatgtt
       61 gacgccgccg tagaccacga aggagcgcag cgggatgtgc ttgccgtagc ccttcacgct
      121 ctcgtgcacc tggatcgcga gctcgcgtgt cggcgtgagg atgaggcagc gcaccggatg
      181 cagcgcgggc gagaagctct tcgtcgccat cggcgcgagg cgctgcagga gcggcagggt
      241 gaagccggcg gtcttgccgg tgccggtctg cgcggcgccg agcaggtcgc gcctcgaaag
      301 gacgatggga atggcctgcg actggatggg ggtgggctct gtgtaacctt gctccgctac
      361 agcgcgaagc agctcgggca acaagccgag gctttcgaat gacgtgctta tgactatctc
      421 ctgatgatcg gcccgcgaag gcatggcctt cgacaccggt tcaggcagac gaaatattga
      481 ctattgggaa ttcgatgctc ggatctgagc tgcgaatcgg tggcggcgcc aaccggatga
      541 gacgccgaac tacacctgga taggcattat accggacaaa tcctcgattc cctattgatt
      601 ttgcggggct ttctcacggc tgtcgcggtg ggctatattc gccctctgcc cggcgatatc
      661 ccgaaccttg aaaaaggagg acgaaaatga agcgcatccc ctggttggca ttggtcgcgg
      721 cggcgggcat cggcaccgcc acggcgcagg aacccatcgc gatcggcgtg agcatcgcgc
      781 aatcgccgcc gggctccgtg gtgcagggca cccaggtgaa ggacggcgtc gagatcatga
      841 aggacatgat caacgcgcag ggcggcgttc tcggccgccc gatcaagctc atctacgagg
      901 acaaccaggg cgttcccgag aagggccgcg ccgccaccga gaagctcatc accaaggaca
      961 aggtggtcgc cgtcaccggc ggacaccaga gctccgtgtg cctcgccgag atcgaggtcg
     1021 cgcatcgcta cgagacgcca tacgtcaata ccaactgctg gtccgacgac gtgcgcaaga
     1081 agggctacgc cgaggtcttc aatccatcca actacaacac gcgcgtctcg accgcgatgg
     1141 cggagacgat cgccgcgatg ggcgtgaagc gcgtcgtcgc gttcgccgag aacaccgact
     1201 acggcatcgg ccaggcgaag ctcatggccg agttcatcaa ggcgaaggcg ccgaacgtgc
     1261 agttcaagta cgagacgctc gatcgcgccg ggaaggactt cacgcccgcg gtggtggcct
     1321 tgcgcgccga tccgcctgac atggtcgtca acatcatgct gccgcccgcg gcgtacatcc
     1381 tgatgaacca gctctacgag cagggcgtgg cgccgagcgc gaagacgtgg tcgtacgacg
     1441 gcgccggcat cgccgactac cccgacttct ggcagaacgt gaaggacgcc ggcaagtaca
     1501 tggtgtcgtt cgggctctac catccgatga tgccgatgcc gaaagtcggc aaggacgtcg
     1561 cggccgaata caccaagcgc acgaagaacg agcccaaccg cctcatcttc caggccgccg
     1621 attcgctgct gctcatcgcc gacgcgatca agcaggcgaa gagcgccgac ccgaaggcca
     1681 tcacgaaggc gctgcagacg atgaagtacg acggcgtgcg cggccccttc cagttcagcc
     1741 acgagccggg ctacgcgttc cagcagtggg tcgacatccc gtacgtcaac taccagctca
     1801 ccgacgtgaa ccagccggtg tcgaagtcca acctcatcca ggggccgggg cagccgctcg
     1861 ccgtgagcaa gctgcagaag ccgaagtaat ccactgtgga gttgcgcgac acgctgatcg
     1921 gcttcgccga ctggcagggc tacggccacg gcgagaggtc cgagtcgtgg ctgcagcgcc
     1981 tcgagttgaa tcgccccgag atcgaggcgt ttcgcggcgc gcgggttccc gatgcgccgc
     2041 cctacgtggc gtgcgccgcg gagctcctgc cggccgaggg cgtcgccgcg cccgatgtga
     2101 agcgccccgc cgatcccgcc gctgcgctcg cgcgcgcgcg cgcggcagcg cgcttgaatg
     2161 cgttcacgca tctgcccgat ggcgtcgaag cgcggggcgt ggggcttctc gccggcatgc
     2221 cgatcgtcgt caaggacctg atgcacgtgg cggggatgcc gctcagcggt ggcggcaagg
     2281 tcatggggcg cccgatggcc acgcgcgatg cggagatcgt cgcgcgcctg cggcgcgcgg
     2341 gcgcggtggt cgtcgggctc gcgaacctgc acgagttcgc ctacggcatc acgagcgaca
     2401 acccgcactt cgggcgcgtc gtgaatccgg tcgcgccgac gcgcatcccg ggcggatcga
     2461 gcggcggctc ggccgccgcg gtggccgcgg gcatcgtgcg cttcgcggtc ggcaccgata
     2521 ccgcgggctc cattcgcgtc ccggcggcgt gctgcggcgt cgtcggattc aagccgagct
     2581 acgacgctct cccgaggggc ggcgtgatcg acctcgcctg ctcgctcgac catgtgggcc
     2641 cgatgacgtt cgatgtcgag gattgcgcgc tgatgttcgc ggcgatgctc gatctccagg
     2701 cggcgccgcg ttgggtccgc aaggacctcg tcggggtgac cgcggcgcgc ctgggcggat
     2761 acttcgccga gccgctcgat cgcgaggtgg cgatggccta cgacgtggcc atgcgcgcgc
     2821 tggcccgcga cggggcgcgc tgcatcgaca cctcggtcga gggcgtcgcg ctggcgccgg
     2881 cgatccagct gaacacgatc gcaccggagg cgacggcata ccacgccaat ctcctcgcat
     2941 cgcgcggggc tgaatacggc gaggacgtgc gcgtgcgcct cgagatgggg ctcttccttc
     3001 ccggccactg gtacgccaag gcgcagcgca tgcgcaccga gctcgtcgat cgcatcgaag
     3061 ccgcgttcgg cgaggccgac gtgctggtgt gtcccacgat gcgcgcgccg gcgccgccgg
     3121 tgggcgccct gcgcatcgag atcggccgcc aggcgttcgc gctgcacacc gcggtcacca
     3181 acctcacgct gcctttcaac c
//
LOCUS       DASVII010000661         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_318187,
            whole genome shotgun sequence.
ACCESSION   DASVII010000661 DASVII010000000
VERSION     DASVII010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318187"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VF309_08565"
     CDS             <1..883
                     /locus_tag="VF309_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX7466681.1"
                     /translation="EILERHAEDSQAFARALPRLAQILERHREAALDQDAEIRELRER
                     EANLTAVREASLAIAHRLAAASYPQEAAAYLYGRYRAVLLHDFRNGGEQSSNWHASLE
                     VLDDILWVLSPRTTPEERARLVSLLPSLLFRLKMGYQRAGLGQDDANLRVEELKQLLD
                     EVMRSPVAAAHGALRKVPAPMPADDFTATLHVNSASLAEEGLARGVWFEFAEDDGAKH
                     RARLNWMSPVQGTCVFKDLDRNRSFAISVDDLREKRRAGRAVIVDGPGVAQSSIEGAI
                     ADVARGLGGSPEARPGA"
     gene            949..>1601
                     /locus_tag="VF309_08570"
     CDS             949..>1601
                     /locus_tag="VF309_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7466682.1"
                     /translation="MLNRPEKLNALTRPMWKRLGGVFEELSADDGVRCIVIRGAGTKA
                     FAPGNDISEFATERSNVEQARAYGADMRRTIEAIDRCPHPVVAQIHGICVGGGLEIAG
                     LADIRICGESSRFGVPINRLGLVMAYAEIGSLIALAGEATALEILLEGRVFDAREAKD
                     KGLVTRVVADDQVEAEARAAAQNIADGAPLVARWHKKFARRLRDPRPLSAEEYDEGFA
                     "
BASE COUNT          243 a          578 c          560 g          220 t
ORIGIN      
        1 cgagatcctc gagcgccacg ccgaggattc ccaggcgttc gcgcgcgcgc tgccgcgcct
       61 cgcgcagatc ctcgagcgcc accgcgaggc cgcgctcgac caggacgccg agatccgcga
      121 gctgcgcgag cgcgaggcca acctcaccgc cgttcgcgaa gcctcgctcg cgatcgccca
      181 tcgcctggcc gcggcgtcgt atccgcagga ggcggccgcc tatctctatg gccgctatcg
      241 cgccgtcctg ctgcacgatt tccgcaacgg cggcgagcag tcgtcgaact ggcacgcgag
      301 cctcgaagtg ctcgacgaca tcctgtgggt gctctcgccg cgcaccacgc cggaggagcg
      361 cgcgcgcctg gtgtcgctcc ttccgtcgct cctcttccgc ctgaagatgg gctaccagcg
      421 cgccggcctc gggcaggacg acgcgaacct gcgcgtcgag gagctgaagc agctgctcga
      481 cgaggtgatg cgctcgccgg tcgccgcggc gcacggcgcg ttgcgcaagg tgccggcgcc
      541 catgcccgcg gacgacttca ccgcgacgct gcacgtgaac agcgcctccc tcgccgagga
      601 agggctcgcg cgcggcgtgt ggttcgagtt cgccgaggac gacggcgcga agcaccgcgc
      661 ccggctcaac tggatgagcc cggtgcaggg cacgtgcgtg ttcaaggacc tcgatcgcaa
      721 ccgctccttc gcgatttcgg tcgacgacct gcgcgagaag cgccgcgccg ggcgcgccgt
      781 gatcgtcgac ggccccggag tcgcgcaatc gtccatcgag ggcgccatcg ccgacgtggc
      841 gcgcggtctc ggcggctcgc ccgaggcgcg ccccggcgcg tagggacgcg gaccatcagc
      901 gcggaacaga acgtcctcac gcagcgcgac ggcgccatcg cgacggtggt gctgaatcgc
      961 cccgagaagc tgaacgccct cacgcggccc atgtggaagc ggcttggcgg cgtgttcgag
     1021 gagctctccg ccgacgatgg cgtgcgctgc atcgtgatcc gcggcgcggg cactaaggca
     1081 tttgcccccg gaaacgacat ctcggaattc gcgacggagc gctcgaacgt cgagcaggcg
     1141 cgcgcttacg gcgcggacat gcgccgcacc atcgaagcca tcgatcgatg cccccacccg
     1201 gtcgtcgcgc agatccacgg gatctgcgtg ggcggcggcc tcgagatcgc cgggctcgcc
     1261 gacatccgca tctgcgggga gtcgagccgc ttcggcgtgc cgatcaacag gctcgggctg
     1321 gtgatggcct atgcggaaat cggctcgctc atcgcgctcg cgggcgaagc gaccgcgctc
     1381 gagatcctgc tcgaaggccg cgtcttcgac gcgcgcgagg cgaaggacaa gggactcgtg
     1441 acgcgcgtcg ttgccgacga ccaggtcgag gccgaagcgc gcgccgccgc gcagaacatc
     1501 gccgatggcg cgccgctcgt cgcgcggtgg cacaagaaat tcgcgcgccg ccttcgcgat
     1561 cccaggccgc tcagtgccga ggaatacgac gagggtttcg c
//
LOCUS       DASVII010000662         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_354647,
            whole genome shotgun sequence.
ACCESSION   DASVII010000662 DASVII010000000
VERSION     DASVII010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354647"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VF309_08575"
     CDS             <1..575
                     /locus_tag="VF309_08575"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HEX7466683.1"
                     /translation="LDVLNAQQGLERLTTYLIGTWNTIPQKEGYGDSTPVRMRIARIW
                     PERTDGYWLYWEYVNPADERQVTRQRIFRFAREGSTIRAVMYRLPGNPAAYAGEWRKE
                     RPFGSLRPDSLRELQGCAAVWDRQFEIYFASGTPGDACPGDRAEIRNEHSEFFLGPSS
                     LRAWITGQDASGKQVDGPSGPSEFRKYLQK"
     gene            689..1318
                     /locus_tag="VF309_08580"
     CDS             689..1318
                     /locus_tag="VF309_08580"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7466684.1"
                     /translation="MPRIKNLFVMAAAAVAACVAVPASADIYSFKDERGVVHFTNIKG
                     IDSRYRLVRREDGSPIKGYSDSAARAYVPSESDIQRYSSIIQTASKTYGVEPSLVHAV
                     ISAESAYNQYAVSRTGAMGLMQLMPDTARRYGVQNMMDPAQNIQGGVRYLADLLVMFK
                     GRIDLAIAAYNAGENAVIRYGLTIPPYAETRHYVPRVLAFYHSFQTRKG"
     gene            complement(1367..1582)
                     /locus_tag="VF309_08585"
     CDS             complement(1367..1582)
                     /locus_tag="VF309_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX7466685.1"
                     /translation="MATMTVESGEEIKRRISELNLEHRDLDRAIEALEQNPLHDELQL
                     KRLKKRKLMLKDQIFMLQRQLVPDIPA"
     gene            1761..>2261
                     /locus_tag="VF309_08590"
     CDS             1761..>2261
                     /locus_tag="VF309_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466686.1"
                     /translation="MNFRRSDFDVTNRINTTDPVCVKLEVIRIFRALYSRNQAPILNR
                     AFDDVVSLYRGQFPGYTRCDTEYHDLQHILDVTLAMARLLDGYERSRGDGPAIGERNY
                     QLGVICGLYHDMGYIRRVNDKKHLNGAEYTAIHVSRGAKFLREYLPTIGMEDVAEVAG
                     AIVHFTG"
BASE COUNT          429 a          708 c          716 g          408 t
ORIGIN      
        1 cgcttgacgt gctcaacgcg cagcagggcc tcgagcgcct cacgacctat ctcatcggca
       61 cctggaacac gattccgcag aaggaagggt acggcgattc cacgccggtg cgcatgcgca
      121 ttgcgcgcat ttggcccgag cgcacggacg gatactggct ctactgggag tacgtgaatc
      181 cggccgacga gcgccaggtg acgcggcagc gaatcttccg gttcgcgcgt gagggatcga
      241 cgatccgcgc tgtgatgtat cggctgccgg ggaatcccgc cgcctatgcc ggggagtggc
      301 gcaaggagcg cccgttcggg tcgctgaggc ccgacagcct gcgcgagctc cagggatgcg
      361 ccgccgtgtg ggaccgccag ttcgagatct acttcgcgag cgggacgccg ggggacgcct
      421 gccccgggga ccgtgcggag atccgcaacg agcactccga gttcttcctc ggccccagct
      481 cattgcgggc ctggatcacc ggccaggacg cctcgggaaa gcaggtggac ggccctagcg
      541 gaccctcgga attcagaaaa tacttgcaga agtaagccta actcattgat ctgtaagaaa
      601 gcccattacg gctgtcattt tcaagtcggc ggggttatgg gttaaaatga aaggctctgt
      661 aggaattgac gaagcgaggt gtaggacgat gccccgaatc aagaacctgt tcgtgatggc
      721 cgccgcggca gtcgccgcct gcgtggccgt gcccgcttcc gccgacatct actccttcaa
      781 ggatgagcgg ggcgtcgtgc acttcacgaa catcaagggc atcgacagcc gctaccggct
      841 cgtgcgccgg gaggacggct cgccgatcaa gggctattcg gatagcgccg cgagggccta
      901 cgtgccgtcg gagagcgaca tccagagata ctccagcatc atccagaccg cgagcaagac
      961 gtacggagtc gagccttcgc tcgtccatgc ggtcatctcg gcggagtccg cgtacaacca
     1021 gtacgccgtc tcgcgcaccg gcgccatggg gctcatgcag ctcatgcccg atacggcgcg
     1081 ccgctatggc gtgcagaaca tgatggatcc ggcgcagaac atccagggcg gcgtgcgcta
     1141 cctcgccgac cttctcgtga tgttcaaggg ccgcatcgat ctcgccatcg ccgcttacaa
     1201 cgcgggcgag aacgcggtca tccgctacgg cctcacgatt cccccctatg ccgagacgcg
     1261 ccattacgtg ccgcgagtgc tcgccttcta ccacagcttc cagacccgga agggctgagg
     1321 gaaatcccga tttgcttggt ggggcggagt acggcccgag gggccgctac gcgggaatgt
     1381 cggggaccag ttggcgctgc agcatgaaaa tctggtcctt cagcatgagc ttgcgtttct
     1441 tgaggcgctt cagctgcagc tcgtcgtgga gcggattctg ctcgagagcc tctatggccc
     1501 ggtccaggtc gcgatgctcc aggttgagct cgctgatgcg ccgctttatc tcttcgccgc
     1561 tttccacagt catggtcgcc atgggcgcga ggcctccgga ggttttaaaa accttactcc
     1621 ccgtggaggt ctttggctag tccgccggaa cgcttgcgga tacttgcggc agcaatgcaa
     1681 atgcgttcca atgcgcaatc ggcgatgggc ttcggcgttt tcagaaagcc cacgaaaaac
     1741 atggcattgc gaaacggcgc atgaactttc gacgtagcga cttcgacgtg acgaaccgga
     1801 tcaacacgac cgatccggtg tgcgtgaagc tcgaggtcat tcgcatcttc cgcgcgctgt
     1861 actcgcgcaa ccaggcgccg atcctgaatc gcgcattcga cgatgtcgtg agcctgtatc
     1921 gcggccagtt tcccggctat acgcgctgcg ataccgaata ccacgacctg cagcacatcc
     1981 tggacgtgac gctcgccatg gcgcggctcc tcgacggcta cgagcgatcg cgaggcgacg
     2041 gcccggcgat cggcgagcgc aactaccagc tcggcgtgat ctgcgggctc tatcacgaca
     2101 tgggctacat ccggcgcgtg aacgacaaga agcacctgaa cggcgccgag tacaccgcga
     2161 tccacgtctc ccgcggggcg aaattcctgc gcgaatacct gccgaccatc ggcatggagg
     2221 atgtcgcgga ggtggcgggc gcgatcgtgc atttcacagg c
//
LOCUS       DASVII010000663         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_714380,
            whole genome shotgun sequence.
ACCESSION   DASVII010000663 DASVII010000000
VERSION     DASVII010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714380"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /gene="mutL"
                     /locus_tag="VF309_08595"
     CDS             <1..1456
                     /gene="mutL"
                     /locus_tag="VF309_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX7466687.1"
                     /translation="IVSRREADRHAWRIACEAGQVSAVEPAALAAGTTVEAEDLYFNT
                     PARRKFLKSEATEFARCDEAYSRIALSRPGIAFSLTHNGRRSSHLPPEGLRERAARVV
                     GEEFARDAIEVSSESTRARLGGLVAPPGFTLASREAQYLFVNGRFVRDKVVAHAIREA
                     YADVLHHDRHPAYVLFLDVDPALVDVNVHPAKSEVRFRESNAMHQFVFHSLARALAAP
                     LAAAGLPRAGGDPGLDSRLRGNDGLARGNDGLGAFQQGGLEVAQPSRGYEAFFAGALV
                     DERRGMAQAAAPAQTPTLGFALAQLQGVYILAQNMAGLVIVDMHAAHERIVYEGLKDA
                     LEAASLPSQPLLVPIQMTATAEEVEQAGEGRAELESLGFDVAASGPRELVIRAVPALL
                     ADLDSQGLLRSVLREMREFGASRVLMERRNELLSTMACHAAVRANRSLTTPEMNALLR
                     QMEQTERAGSCNHGRPTWHQLAMSDLDRLFMRGR"
     gene            1453..>1712
                     /locus_tag="VF309_08600"
     CDS             1453..>1712
                     /locus_tag="VF309_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX7466688.1"
                     /translation="MKAVFLLGPTASGKTAVALALAERFPVEIVSVDSAQVYRGMDVG
                     TAKPGAEDRARVPHHLIDIIEPTEAYSAGRFRSDALRIAREI"
BASE COUNT          246 a          599 c          607 g          260 t
ORIGIN      
        1 gatcgtgagc cggcgcgaag ccgatcgcca cgcgtggcgc atagcatgcg aggcgggaca
       61 ggtctccgcc gtcgagcccg cggccctcgc cgccggcacc acggtcgaag ccgaggacct
      121 ctatttcaat acgccggcgc ggcgcaagtt cctgaagagc gaggcgaccg agttcgcgcg
      181 ctgcgacgag gcgtactcgc gcatcgcgct gtcgcgcccc gggatcgcct tctcgctcac
      241 gcacaacggg cggcgctcgt cgcacttgcc tcccgagggg ctgcgcgagc gcgcggcacg
      301 ggtcgtcggc gaggaattcg cgcgcgatgc gatcgaggtg agctccgaga gcacccgcgc
      361 ccgcctcggc ggcctcgtgg cgccgcccgg attcacgctc gcctcgcgcg aggcccagta
      421 tctgttcgtg aacgggcgct tcgtgcgcga caaggtcgtc gcccacgcga tccgcgaagc
      481 ctacgcggac gtgctgcatc acgaccgcca tccggcgtac gtgctctttc tcgacgtcga
      541 tcccgcgctc gtggacgtga acgtgcatcc ggcgaagagc gaggtgcgct tccgcgaatc
      601 gaatgcgatg caccagttcg tcttccactc gctcgcccgc gcgctcgccg cgccgctcgc
      661 ggccgcgggc cttccccgcg caggcgggga ccccgggctg gattcccgcc ttcgcgggaa
      721 tgacgggctc gctcgcggga atgacgggct cggcgctttt cagcaagggg gcctcgaggt
      781 cgcgcagccg tcccgcggct acgaggcgtt cttcgccggg gcgctcgtcg atgagcgtcg
      841 cggcatggcg caagcggccg cgcccgcgca gacgccgacg ctcggattcg ccctcgcgca
      901 attgcagggc gtctacattc tcgcgcagaa catggcgggc ctggtgatcg tggatatgca
      961 tgcggcccac gagcgcatcg tctacgaggg cctcaaggat gcgctcgagg cggcatccct
     1021 tccgtcgcag ccgctcctcg tgccgatcca gatgacggcg accgcggaag aggtcgagca
     1081 ggcgggagag ggccgcgccg agctcgagag cctgggtttc gacgtcgcgg cgtcgggacc
     1141 gcgcgagctc gtgatccgcg cggtgccggc gctcctcgcg gacctcgatt cgcaggggct
     1201 cttgcgctcc gtgcttcgcg agatgcgcga attcggcgcg agccgcgtgc tgatggagcg
     1261 gcgcaacgag ctcctgtcga cgatggcgtg ccacgcggcg gtgcgggcga atcgcagcct
     1321 gacgactccc gagatgaacg cgctcctgcg gcaaatggag cagacggagc gcgcgggcag
     1381 ctgcaaccac ggtcgcccga catggcacca gcttgcgatg tccgacctcg accggctctt
     1441 catgcgggga cggtgaaggc ggttttcctc ctggggccca ccgcgagcgg caagacggcg
     1501 gtggccctcg cgctcgcgga gcgcttcccc gtggagatcg tgagcgtcga ttccgcgcag
     1561 gtctatcggg gcatggacgt ggggaccgcg aagcccggcg ccgaagatcg cgcgcgcgtt
     1621 ccccatcatc tcatcgacat tatcgagccg accgaagcct attcggcggg gcgcttccgt
     1681 agcgacgccc tgcgaatcgc gcgcgagatc ca
//
LOCUS       DASVII010000664         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_498378,
            whole genome shotgun sequence.
ACCESSION   DASVII010000664 DASVII010000000
VERSION     DASVII010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498378"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1686)
                     /locus_tag="VF309_08605"
     CDS             complement(<1..>1686)
                     /locus_tag="VF309_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7466689.1"
                     /translation="ASEIASHAPVDAARLEEAGIRAIAGFPVVVDGQVTAALILIAEQ
                     ASDLVYLDSLRTVVRIFASALDKKRLDLALAWRLRLTELIGSTSRRFIDVPLAELRAS
                     IDEALHQIAALCDFKRAVLYELSDDRKRFSITHRYLASGIEPTEPAGVEQPVEHFGYV
                     ASRLAMKQPIIITADGLPKEATTERRELAVGGMTHAVVFPLVVGDDALGAVSFASVAT
                     PDPTVVEMLGVVGELLTSALHRERVERGLIERLEEISRLKRQIEGERDYLREEVRESR
                     QLGDLVGPSAALGRVVALVESVASTSAAVLIRGESGVGKELIARAIHARSQRSEEPLV
                     KVNCASVPKELFESEFFGHVRGAFTGALKDRAGRFELADRGTLFLDEVGEIPYDLQAK
                     LLRVLQEKEFERVGGDKTRKVDVRIVAATNRDLAAEAAAGRFRKDLYYRLSVFPIEIP
                     PLRDRREDIMPLAEHFLKVQRRNLGRPHLVFGDEHRRRLLAYDWPGNVRELQNVIERA
                     VIICDSGTLTVDPHWLSRETAKPVRPLGDELAARERQMIETALAESRGRVSGP"
BASE COUNT          277 a          556 c          579 g          274 t
ORIGIN      
        1 tcggtcccga gacgcgccca cggctctcgg caagcgccgt ctcgatcatc tgccgctcgc
       61 gtgcggccag ctcgtcaccg agtggccgca ccggcttcgc cgtctcgcgc gagagccagt
      121 gcgggtcgac ggtgagcgtg ccggagtcgc agatgatgac cgcccgctcg atcacgttct
      181 gcagctcgcg cacgttgccg ggccagtcgt acgcgaggag gcgacgccgg tgctcatcgc
      241 cgaacacgag gtgcggcctc cccaggttgc ggcgctgcac cttcagaaag tgctcggcga
      301 ggggcatgat gtcttcacgc cggtcgcgca gcggagggat ctcgatggga aagacgctga
      361 gccggtagta gaggtccttg cggaagcgcc cggcagcagc ttcggcggcg agatcgcggt
      421 tggtggccgc gacgatacga acgtcgacct tgcgcgtctt gtcgcctccg acgcgctcga
      481 actccttctc ttgaagaacg cggagcagct tggcctgcag gtcgtaaggg atctcgccga
      541 cctcgtcgag aaagagggtg ccccgatcgg cgagctcgaa tcgcccggcg cgatccttga
      601 gcgcgccggt gaatgcgccg cgcacgtgcc cgaagaactc gctctcgaag agctccttcg
      661 gcaccgaggc acagttgacc ttgaccagcg gctcttcgga gcgctggctg cgggcgtgga
      721 tggcgcgggc gatgagctcc ttgcctaccc ccgattcgcc gcgaatgagc accgcagccg
      781 acgtgctcgc cacgctctcg acgagggcca ccacacgacc gagtgccgcg ctcggtccta
      841 ccagatcgcc aagctgccgg ctctcgcgga cttcttcgcg caggtagtcg cgctcgccct
      901 cgatctgtcg tttcaatcgg gagatctctt cgaggcgctc gatgagccct cgctcgacgc
      961 gctcgcggtg cagcgcgctc gtaaggagct cgcccacgac gccgagcatc tcgacgacgg
     1021 tcggatcagg cgtcgcaacg ctggcgaagc tcaccgcgcc gagggcgtcg tctcccacca
     1081 cgagcggaaa gacgacagcg tgggtcatcc ctcccactgc cagctccctg cgttcggtcg
     1141 tggcctcttt gggcaagccg tcagcggtga tgatgatggg ctgcttcatg gcaaggcgcg
     1201 aggcaacgta gccaaagtgc tccacgggtt gctcgacccc agcgggctcc gtcggctcga
     1261 tgcccgacgc gagatagcgg tgggtgatcg agaaacgctt ccgatcgtcg gagagctcgt
     1321 acaagacggc gcgcttgaag tcgcagagcg cggcgatctg gtggagggcc tcgtcgatgc
     1381 tcgctcggag ctcggccagt ggaacatcga tgaaccggcg cgaggtgctg ccgatcagct
     1441 cggtaaggcg caggcgccag gcaagcgcca gatcgaggcg tttcttgtcg agcgcgctgg
     1501 cgaagatgcg caccacggtc cgcaagctgt cgaggtagac gagatccgac gcctgctcgg
     1561 cgatcaagat gagggcagcc gtcacctgtc cgtcgaccac cacggggaag ccggcgatgg
     1621 cgcgaatgcc ggcctcctcg agccgggcgg cgtcgacggg agcgtgcgag gcgatctccg
     1681 atgcat
//
LOCUS       DASVII010000665         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_678231,
            whole genome shotgun sequence.
ACCESSION   DASVII010000665 DASVII010000000
VERSION     DASVII010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678231"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VF309_08610"
     CDS             <1..633
                     /locus_tag="VF309_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7466690.1"
                     /translation="GRLAGDDDIPVDRRVSAVLERRREVFESLGCIVEEAHPDLSGAD
                     EVFHVLRALGFVMRYGELAKTHRGEIKETVIWNVEEGLKLAPARIAAAMALRSELYRR
                     MREFMERYEFLCLPVNQVPPFPVSQPYVDAINGVKLASYIDWMKSAYYITTTSHPAIS
                     VPAGFTGDDPPLPVGLQIVGRYRDDFGVLQMAHAFEQETRVGERGPTLAQ"
     gene            647..1147
                     /locus_tag="VF309_08615"
     CDS             647..1147
                     /locus_tag="VF309_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466691.1"
                     /translation="MKGLAAGAFTIAVAVAPGALADNFWHPAPSGADPFWHPAPSAAD
                     PFWHPAPHAAVPVPQNGVHPGVHPVVRNPLWRRPVRAVQQPRFRFRGQTFVVIGAPLY
                     AAPWLSYPYALGTPWGASYYVPGEQGYFLYYCVDPAGYYPEVQDCPGGWWQTVPEEPQ
                     ETDPNY"
     gene            1190..>2404
                     /locus_tag="VF309_08620"
     CDS             1190..>2404
                     /locus_tag="VF309_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7466692.1"
                     /translation="MKDLTQGPIPRHVLTMAVPIAAGMLLQTLYFIVDLYFVARLGDA
                     AIAGVSAAGNVMFVVFALTQTLGVGTVALVSHAVGRKDRDEANHVFNQSVALAALCAL
                     FTLVAGYGLADGYMGFFGADAATKEAGVRFLDTFIPGLALQFALVVMGSGLRGTGIVQ
                     PTMVVQALTVVLNAALAPVLIAGWGTGMPMGVAGAGLASSLSVAAGVVLMTVYFLRLE
                     KYVGFDASQWRPRVATWKKLLNIGLPAGGEFLFIGVYTGVIYWIVRDFGTPAQAGFGI
                     GSRVIQMIFLPAMAVAFAAAPIAGQNYGAKQAVRVRETFASAAIASCAIMFSMTLLCQ
                     WQGEAMIGIFTSDAAVIAVAAQFIHVISWNFFAMGIVFTCSSLFQALGNTWPALLSTG
                     TRLLTFAGPAIFL"
BASE COUNT          350 a          803 c          818 g          433 t
ORIGIN      
        1 ggccgcctcg ccggcgacga cgacatcccg gtggaccggc gcgtgagcgc cgtcctcgag
       61 cgtcgccgcg aagtgttcga gtcgctgggc tgcatcgtcg aggaggcgca tcccgacctg
      121 agcggcgccg acgaggtctt ccacgtgctg cgcgcattgg gcttcgtgat gcgctacggc
      181 gagctcgcga agacccatcg aggagaaatc aaggagaccg tgatctggaa cgtcgaggag
      241 gggctcaagc tcgcgcccgc gcgcattgcc gcggcgatgg cgctgcgctc cgagctgtat
      301 cggcgcatgc gcgagttcat ggagcgctac gaattcctgt gcctgccggt aaaccaggtg
      361 ccgccgttcc ccgttagcca gccatacgtc gacgccatca acggcgtgaa gctcgcgagc
      421 tatatcgact ggatgaagag cgcttactac atcacgacca cgagccaccc ggcgatctcg
      481 gtgcccgcgg gattcaccgg cgacgatccg ccgctgccgg tcgggctgca gatcgtcggc
      541 cgctatcgcg acgatttcgg cgtcttgcag atggcgcacg cgttcgagca ggaaacccgc
      601 gtgggcgagc gcggtccaac attggcgcaa tagcgcgggg gaatccatga aaggtctggc
      661 agcgggagct ttcacgatcg cggtcgccgt cgcgccgggc gcgttggcgg acaatttctg
      721 gcatccggcg ccgagcgggg cggatccctt ctggcatccc gcgccgagcg cggcggatcc
      781 cttctggcac cccgcgcccc acgccgcggt acccgtgccg cagaacggcg tccaccccgg
      841 agtgcatccc gtcgtgcgca accccctgtg gcgccgtccc gtgcgcgcag tccagcagcc
      901 gcgctttcgc tttcgcggcc agaccttcgt ggtgatcgga gcgccgctct acgccgcgcc
      961 gtggctttcc tatccgtatg cgctcggcac gccgtggggt gcgagctatt acgttcctgg
     1021 cgagcaggga tacttcctgt actactgcgt cgatcccgcc ggctattacc cggaggtcca
     1081 ggattgcccg ggcggctggt ggcaaaccgt gcccgaagag ccgcaggaga cggacccgaa
     1141 ctactgagcg gccgcgatcc ggcggcgatt gccggataga attcgcggca tgaaagatct
     1201 cacgcaaggc cccatccccc ggcacgtgct cacgatggcg gtgcccatcg ccgcgggcat
     1261 gctgctgcag acgctctatt tcatcgtcga cctctacttc gtcgcgcggc tcggagacgc
     1321 ggcgatcgcc ggcgtgagcg ccgcgggcaa cgtgatgttc gtcgtgttcg cgctgacgca
     1381 gacgctcggc gtcggcaccg tagccctcgt ctcccatgcg gtcggccgca aggatcgcga
     1441 cgaggcgaac cacgtcttca accagtcggt ggcgctcgcc gcgctctgcg cgctcttcac
     1501 actcgtcgcg ggctatggcc tcgccgacgg ctacatgggc ttcttcggcg ccgacgcggc
     1561 aaccaaggag gcaggcgtgc gattcctcga cacgttcatt ccggggctcg cgctgcagtt
     1621 cgcgctggtg gtgatgggct cgggattgcg tggcaccggc atcgtccagc cgacgatggt
     1681 ggtccaggcc ctcacggtgg ttctcaacgc cgcgctcgcg ccggtcctga tcgcgggatg
     1741 gggcacggga atgcccatgg gcgtggcggg agcgggcctc gcgagctcgc tatcggtcgc
     1801 ggcgggcgtc gtcctgatga cggtctactt cctgcgcctc gagaagtacg tgggattcga
     1861 cgcctcgcaa tggcgcccgc gcgtcgcgac ctggaagaag ctgctgaata tcgggcttcc
     1921 cgcgggaggc gaattcctct tcatcggcgt ctataccggc gtgatctact ggatcgtccg
     1981 cgacttcggc acaccggcgc aggcgggctt cggcatcggc tcgcgcgtga ttcagatgat
     2041 cttcctgccg gcgatggcgg ttgcgttcgc cgcggcgccg atcgcggggc agaactatgg
     2101 cgcgaagcag gccgttcgcg tgcgggagac gttcgcctcg gccgcgatcg cgagctgcgc
     2161 gatcatgttc tcgatgacgc tcctttgcca gtggcagggc gaggcgatga tcggcatctt
     2221 cacctcggac gcggcggtga tcgcggtggc cgcgcagttc atccacgtga tctcgtggaa
     2281 tttcttcgcg atggggatcg tgttcacgtg ctccagcctg ttccaggcgc tcggcaacac
     2341 ctggccggcg ctattgagca cggggacgcg cctcttgacc ttcgcgggcc ccgcgatctt
     2401 cctt
//
LOCUS       DASVII010000666         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_210611,
            whole genome shotgun sequence.
ACCESSION   DASVII010000666 DASVII010000000
VERSION     DASVII010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210611"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VF309_08625"
     CDS             complement(<1..1018)
                     /locus_tag="VF309_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7466693.1"
                     /translation="MTKLSIALVQVLGVGLAASVATLPAMAQQPASKERIEVTGSNIK
                     RVEGETALPVTVITREEIQNSGVQTVQDILERLPTNMSFGSFNEAGGEGSALVGFTGA
                     SLRGFGTNRTLVLINGKRVAPYALSNQIGGGADLSAIPVSVIERVEILKDGASAVYGT
                     DAIAGVINFILRKDFRGVEATATLLDTEHGGAKQERFTGSIGFGDLSKDRWNVFLTAD
                     HLKQDPLKAADRAISHTAYLPALAYDRTSGNSFPANISQPGGFSGTRNPTIPFPGGAN
                     STSCIPPFSFPTAGSPFQCRFDFASVIETIPNFTKDNFMGRAAIQLAPDHQAFVEGSY
                     YKGTF"
     gene            1497..1925
                     /gene="rimP"
                     /locus_tag="VF309_08630"
     CDS             1497..1925
                     /gene="rimP"
                     /locus_tag="VF309_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7466694.1"
                     /translation="MSEREAWIRATLEGLGYELVDLESSRSGLLRIFIDSPRGITVED
                     CAKVSNHLTRAFVVEGIDYERLEVSSPGLDRPLKTLGDFERFAGRDVTLKLKLPRDGR
                     RRFEGKLVGVEGGRIAVDVEGQRQHLEFAEIDRARLVPDV"
     gene            1941..3431
                     /gene="nusA"
                     /locus_tag="VF309_08635"
     CDS             1941..3431
                     /gene="nusA"
                     /locus_tag="VF309_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7466695.1"
                     /translation="MSKDLLLLVDALSREKNVAKDTVFLALEMALASATKKRFKDEVD
                     VRVEIDRESGEYSAFRRWTVVPDEEHEEPAHQIAITDAVEENPEAVLGDVIEEPLEPE
                     TFGRIGAQAAKQVILQKIRDAEREQILNDFLERGESLVTGTVKRAERGNLIVESGRVE
                     ALLPRDQLIAKENLRVGDRVRAYIAKIDRAARGPQLILSRVAPEFLVKLFELEVPEIE
                     EGLLEIKAAARDPGLRAKIAVKSNDPRIDPVGTCVGMRGSRVQAVTQELAGERVDIIL
                     WSPEPAQFVINALAPADVSSIVVDEERHSMDVVVTEDQLALAIGKGGQNVRLASELSG
                     WQLNIMTTEERETKSEAETSVVRALFMEKLDVDEEVAEILVKEGFTSLDEVAYVPINE
                     MLEIEDFDEETVNELRRRARNALLTEAIKSEETVEHAAEDLQSMEGMDNPTARLLASK
                     GVTTMDALADLATDELTEMTGMDEERANKLIMKAREPWFKEPQAQA"
     gene            3451..>3584
                     /locus_tag="VF309_08640"
     CDS             3451..>3584
                     /locus_tag="VF309_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7466696.1"
                     /translation="MPKSNVTQFAEELKLPVALLLEQLQAAGVKKKAGEDAITEQDKA
                     R"
BASE COUNT          674 a         1067 c         1227 g          616 t
ORIGIN      
        1 cgaacgtgcc cttgtaatac gagccttcca cgaaggcctg gtggtcgggc gcgagctgga
       61 tcgcggccct gcccatgaag ttgtccttcg tgaaattcgg gatcgtctcg atcacgctcg
      121 cgaagtcgaa gcggcactgg aacggcgagc cggcggtcgg gaacgagaag ggcgggatgc
      181 acgaggtgga gttggcgccg cccgggaagg gaatggtcgg gttgcgcgtg ccgctgaatc
      241 cgcccggctg gctgatgttc gcgggaaacg agttgcccga ggtgcggtcg tatgccaggg
      301 ccgggaggta cgccgtgtgc gaaatcgccc ggtcggccgc cttgagcgga tcctgcttga
      361 ggtggtccgc ggtcaggaag acgttccagc gatccttcga aagatcaccg aatccgatgg
      421 agcccgtgaa gcgttcctgc ttcgcgccgc cgtgttccgt gtcgagcagc gttgccgtcg
      481 cttcgacgcc gcggaagtcc ttgcgcagga tgaagttgat aacgcccgcg atcgcatccg
      541 ttccgtacac cgcagaagcg ccgtccttca ggatttcgac gcgctcgatc acggaaacgg
      601 gaattgcgga aaggtcggcg ccgccgccga tctggttgct gagggcgtac ggggcgacgc
      661 gcttgccgtt gatcaggacg agagtgcggt tggtcccgaa gccgcgcagt gacgcgcccg
      721 tgaagccgac gagcgcgctg ccttcgccgc ccgcctcgtt gaagctgccg aatgacatgt
      781 tggtcggcag gcgctcgaga atgtcctgga cagtctggac gccggaattc tggatctcct
      841 cacgggtgat gaccgtgacg gggagcgcgg tctcgccttc gacgcgcttg atgttggagc
      901 cggtaacttc gattcgctct ttggaggccg gttgttgcgc catcgcgggc agcgttgcaa
      961 cgctggccgc gagacccacg cccagcactt gcacgagcgc aatggaaagc tttgtcattc
     1021 tcggattcat tcgtgaaacc cccaggtagt tataaaatat tagaaaaaac agagacttgc
     1081 ataaggagta aagcgttggt tttgtggtcc ttttttttga tcggcctgat gacacgcacg
     1141 ggataataac gaggcggctg gcacttgacc aacacttatt cgcggagtgt tgcgtttatg
     1201 caacatcaaa aaggtgactc cgacgcccgc ttttgtcgga aatgcgcgcc gcgatcgcat
     1261 ggactagcgc tgtagaagcg ccggctgata cagaaatgcg cggctcgatc gaggcgggcg
     1321 tgctcaaatc gatgttccgg gcgcgcaaaa tcggcgcagc gccggagggg ccgcaaggcc
     1381 cttgattcct cgccgaaagc gacgctttcg ggtgacgttt ttgggcattc cgtgctagaa
     1441 tctgtcggtt tcggagttgg agatgggcct cggcccattt tttgtttgtg agaccgattt
     1501 cagagcgaga agcgtggatt cgggcaaccc tcgaggggct cggatacgaa ctcgtcgacc
     1561 tggagtcgtc gcgctcggga ttgctcagga tcttcatcga ttccccgcgc gggattaccg
     1621 ttgaggattg cgcgaaggtg agcaaccatt tgacgcgcgc cttcgtcgtg gagggaatcg
     1681 attacgagcg gctcgaggtc tcgtcgccgg gtctcgaccg tccgctgaag acgctcgggg
     1741 atttcgagcg tttcgcgggg cgcgacgtga cgctcaagct gaagctgccg cgcgatgggc
     1801 ggcggcgttt cgagggaaag ttggtgggag tcgagggcgg gcgaatcgcc gtcgacgtcg
     1861 agggtcaacg gcaacacctg gaattcgctg aaatcgaccg cgcgcgactc gtgccggacg
     1921 tgtgaggggc ttggagggtc atgagcaagg atctactgct gctggtcgac gcgctgtcgc
     1981 gcgagaagaa cgtcgcgaag gacaccgtgt tcctcgcgct cgagatggcg ctcgcctcgg
     2041 cgaccaagaa gcgcttcaag gacgaggtcg acgtccgcgt cgagatcgac cgcgagtccg
     2101 gcgagtattc cgccttccgc cgctggaccg tcgttcccga cgaggagcat gaggagcccg
     2161 cgcaccagat cgcgatcacg gacgccgtcg aggagaatcc cgaggcggtc ctcggcgacg
     2221 tgatcgagga gccgctcgag cccgagacct tcggtcgcat cggcgcgcag gccgcgaagc
     2281 aggtgatcct gcagaagatc cgcgacgccg agcgcgagca gatcctcaac gacttcctcg
     2341 agcgcggcga gtcgctcgtg accggcacgg tgaagcgcgc cgagcgcggc aacctcatcg
     2401 tcgagtcggg gcgcgtggaa gcgctcctgc cccgcgacca gctcatcgcc aaggaaaacc
     2461 tgcgcgtggg cgaccgcgtg cgcgcctaca tcgccaagat cgaccgcgcc gcccgcggcc
     2521 cgcagctcat cctgtcgcgc gtcgcgcccg agttcctcgt gaagctcttc gagctcgagg
     2581 tgcccgagat cgaggagggc ctcctcgaga tcaaggccgc ggctcgcgat ccggggctgc
     2641 gcgcgaagat cgccgtcaag tccaacgatc cgcgcatcga tcccgtggga acgtgcgtcg
     2701 gcatgcgcgg ctcgcgcgtg caggcggtca cccaagagct cgcgggcgag cgcgtcgaca
     2761 tcatcctgtg gtcgcccgag cccgcgcagt tcgtgatcaa cgcgctcgcg cctgccgacg
     2821 tgagctcgat cgtggtggac gaagagcgcc actcgatgga cgtggtcgtg accgaggacc
     2881 agctcgcgct cgccatcggc aagggcggcc agaacgtgcg cctcgcctcg gagctttccg
     2941 gctggcagct caacatcatg acgaccgagg agcgcgagac gaagagcgag gccgagactt
     3001 cggtcgtgcg cgcgctcttc atggagaagc tcgacgtcga cgaggaggtc gccgagattc
     3061 tcgtgaagga aggcttcacg tcgctcgacg aggtcgcgta cgtgccgatc aacgagatgc
     3121 tcgagatcga ggatttcgac gaggaaacgg tgaacgagct gcgccgccgc gcgcgcaacg
     3181 cgctcctcac ggaagcgatc aagagcgagg agaccgtcga gcacgccgcc gaggacctgc
     3241 agagcatgga gggcatggac aatcccaccg cgcggctgct cgcctccaag ggcgtcacga
     3301 cgatggatgc tctcgccgac ctcgcgaccg acgagctcac cgagatgacc ggaatggatg
     3361 aagagcgcgc gaacaagctc atcatgaaag cgcgcgaacc gtggttcaag gagcctcagg
     3421 cccaggcctg acgcatagga acggaaacga atgcctaagt cgaacgtcac ccaattcgcc
     3481 gaggagctca agctcccggt cgcccttctc ctcgagcagc tccaggccgc gggggtgaag
     3541 aagaaggcgg gtgaggacgc gatcaccgag caggacaagg cgcg
//
LOCUS       DASVII010000667         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_570926,
            whole genome shotgun sequence.
ACCESSION   DASVII010000667 DASVII010000000
VERSION     DASVII010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570926"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VF309_08645"
     CDS             complement(<1..396)
                     /locus_tag="VF309_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7466697.1"
                     /translation="MQRWRAEATQKQHKGIRDAIEEGHVSALARMLATTPVDTVVSAN
                     GRTLLMLAVEEGNLPAIELLVARGAALDLRDQRGATALLLAVEMGFQEAVEALLSAGA
                     DPNVHDLSGMTPLDLTEEHEAQDLAAVLLR"
     gene            complement(388..1119)
                     /gene="pssA"
                     /locus_tag="VF309_08650"
                     /pseudo
     CDS             complement(388..1119)
                     /gene="pssA"
                     /locus_tag="VF309_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
     gene            complement(1130..1777)
                     /locus_tag="VF309_08655"
     CDS             complement(1130..1777)
                     /locus_tag="VF309_08655"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX7466698.1"
                     /translation="MNDYPHPIIAREGWPYLAASLVASVLVTWWFGWWSAPFWILYLF
                     ILQFFRDPPREIPQGANLVLCPADGRVLLVGNMRDPYRNVEALKVSVFMNVFNVHSNR
                     SPVDAEVKDVVYNKGLFVNASFDKASSDNERNALILRMDDGTELACVQVAGLIARRIL
                     CYVKPGERLTRGQRYGFIRFGSRVDLYLPLGSKPRVAIGDKVKATITVIAELPGR"
BASE COUNT          313 a          619 c          635 g          273 t
ORIGIN      
        1 gcgaagcagg accgcggcga ggtcctgggc ctcgtgctcc tcggtgaggt cgaggggcgt
       61 catgcccgag aggtcgtgga cgttgggatc ggcgccggcc gaaagcagcg cctcgaccgc
      121 ctcctggaat cccatctcga cggcgagcag cagcgcggtc gcgccacgct ggtcgcgcag
      181 gtccagcgcc gcgccgcgcg cgaccagcag ctcgatcgcc ggaaggttgc cttcctcgac
      241 cgcgagcatg aggagcgtgc gcccgttcgc cgaaacgacc gtgtcgacgg gtgtcgtggc
      301 gagcatgcgc gctagcgcgg agacgtgccc ctcctcgatc gcgtcgcgaa tgcccttgtg
      361 ctgcttctgc gtcgcctcgg cgcgccagcg ctgcacgacc caggtgacgt agcccgagat
      421 gccgtaggcg agcatcgcgc cccacagcac gtgcgagggc tcgagcgaga tgagcaggag
      481 cgcgaggacc agcagcagca cgacgtagaa cggcaccgcg cgccgcaggt tgatgtcctt
      541 gccgctgtag aacctcacgt tggacaccat cgtgatcccg gcgtacacgg tgacggccgc
      601 ggcgatccac ttcacctcgc ccccgcccac ctggtagtcg ttgaagaccc agatcatccc
      661 cgccaccagc gccgccgcgg ccgggctggg aaggcccgtg aaccagcgct tgtcggcgac
      721 cgaaagctgc gtgttgaagc gcgcgagccg cagcgccgcg cccacgcaat acacgaaggc
      781 cgcgatccag ccgatgcggc ccatgtcgcg aagcgcccac tcgtacatca ccagcgccgg
      841 cgccgcgccg aaggagacca tgtcggcgag gctgtcgtac tcggcgccga acgcggactg
      901 ggtgcgcgtg agccgcgcga cgcgcccgtc gatgctgtcg agcaccatcg cgacgaagat
      961 ggcgatcgcc gacttctcga agtcgtggtt catgccctgc acgatcgcgt agaaccccgc
     1021 gaagaggttc agcgtcgtga atatgttggg cagcaggtag atgccgcgct tgcgcgcgcg
     1081 ctcgatcggc ttcgcgggcg tgctgatgtg cgtggccatg gtcgcgggct cagcggcccg
     1141 gaagctccgc gatgaccgtg atcgtcgcct tcaccttgtc gccgatcgcc acgcgcggtt
     1201 tcgagcccag cggaaggtag agatcgaccc gcgagccgaa gcggatgaac ccgtagcgct
     1261 gcccccgcgt gagccgctcg ccgggcttca cgtagcagag gatgcgccgc gcgatgaggc
     1321 ccgcgacctg cacgcaggca agctcggtgc cgtcgtccat gcgcaggatg agggcgttgc
     1381 gctcgttgtc ggacgacgcc ttgtcgaaag aggcgttcac gaacaggccc ttgttgtaca
     1441 cgacgtcctt cacctcggcg tccaccggcg agcggttcga gtgcacgttg aagacgttca
     1501 tgaagacgct caccttcagc gcctcgacat tgcgatacgg gtcgcgcatg ttgcccacca
     1561 gcagcacgcg gccgtcggcg gggcacagca cgaggttcgc gccctgcggg atttcgcggg
     1621 gcgggtcgcg aaagaactgg aggatgaaga ggtacaggat ccagaaggga gccgaccacc
     1681 atccgaacca ccaggtgacg aggaccgagg cgacgaggga ggcggcgagg tacggccacc
     1741 cttccctcgc gatgatcgga tgcgggtagt cgttcatctc acgccctcgg tggttggatc
     1801 acgcccctcg gccggcatga caaggcctcg tggccgtcag
//
LOCUS       DASVII010000668         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_571042,
            whole genome shotgun sequence.
ACCESSION   DASVII010000668 DASVII010000000
VERSION     DASVII010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571042"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..570
                     /locus_tag="VF309_08660"
                     /pseudo
     CDS             86..570
                     /locus_tag="VF309_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            757..1137
                     /locus_tag="VF309_08665"
     CDS             757..1137
                     /locus_tag="VF309_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX7466699.1"
                     /translation="MGHGRVGGFIGKALAGGGTPLFVIEDDSDKVATLRQGGVEALAG
                     NAADPEIIAAANLGQARCLFVAIPDAFEGGQVVQQARAINPTLWIVARSHSEAESEHL
                     LRHGATLVVMGEHEIAKAMLGQLA"
     gene            complement(1134..>1568)
                     /locus_tag="VF309_08670"
     CDS             complement(1134..>1568)
                     /locus_tag="VF309_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466700.1"
                     /translation="LSGPPQLSPIATYEGRLVSANIVEGPKQKPDYANLPTCVFTIPA
                     LATVGLSEAKANEKSLKTRVQVNDMQYWFSTRTFAETVAWWKIIVDEASDAVVGAHFV
                     GHSGEELVNIFGLAMKHGIKASQIRDFIFAYPTFSADIKHML"
BASE COUNT          239 a          527 c          499 g          303 t
ORIGIN      
        1 gaagggcgcc gcgcaagtcg atctcgcctc agtcgcgctc gaagtcggca tcacaatcgc
       61 caagatcgcg gccttcgtcg ccatcatgct gatcgtcggc aagcgggtca ttccgtgggt
      121 gctgcatacc atggcgcaca ccgggagccg cgagctattc cgcctctcgg tgttcgtgat
      181 cgctctcggc gcggccttcg ccgcagccga gctgttcggc gtctcgttcg cgctcggcgc
      241 cttcttcgcc ggcatgatcc tgagcgagtc gccgctgagc cagcgggcgg cggaggaaac
      301 cttgccgctg tgcgacgcct tcgccgtctt ttcttcgtct cggtcggcat gctgttcgat
      361 ccaaccatcc tcgtgcgcga gccgctggcc gtgctcgcca ccgtgctgat catcctgctc
      421 ggcaagtcgc tcgcggcctt cgccatcgtg cgcgccttcg gctatccgac ctcgaccgct
      481 ctcaccatct cggcatcgct cgcgcagatc ggcgagttct ccttcatcct cgccaatctc
      541 ggggtcagcc tgttgctgct ccccgagcag ggccgtgatc tcatcctcgc cggcgccatc
      601 gtctcgatcc tgttgaaccc gctgttgttc gctgcgctcg accggtggtt cgagtggcgc
      661 gacaaggcga agggcgagga agcgattccc gccgaagacg aagccatagc gcgcgagccg
      721 atccgtccga cgcgccagtc aggccatgtc gtcctggtgg gacatgggcg ggtcggcggc
      781 tttatcggca aggcgctcgc cggcggcggc accccgctct tcgtcatcga ggacgatagc
      841 gacaaggtcg ccaccttgag gcagggaggc gtcgaggcgc tcgccggcaa tgccgccgac
      901 cccgagatca tcgccgcggc gaatctcggt caggcacgct gcctgttcgt tgctattccc
      961 gacgccttcg agggcgggca ggtggtgcaa caagcccgcg ccatcaaccc gacgctgtgg
     1021 atcgtcgccc gctcccactc ggaggccgag agcgagcatc tgctccgcca cggcgccacg
     1081 ctcgtggtga tgggcgagca cgagatcgcc aaggcgatgc tcggccaact cgcctagagc
     1141 atgtgtttga tgtcggccga gaaggtcgga taggcgaaga tgaaatctcg gatctggctc
     1201 gctttgatgc cgtgcttcat ggcgagcccg aaaatgttga ccagctcctc gccggaatgg
     1261 cccacgaaat gcgcgccgac gaccgcgtcg ctcgcctcat cgacgatgat cttccaccag
     1321 gcaaccgtct cggcgaaggt tcgcgtcgag aaccaatatt gcatgtcgtt gacttggacg
     1381 cgcgtcttga gacttttctc gttggctttg gcctcggaca ggccgacagt ggcgagcgcc
     1441 gggatggtga agacgcaagt cggcaggttc gcatagtcgg gcttctgctt cggcccctcg
     1501 acgatgttgg cgctgaccag ccgcccttca taggtcgcga tcggcgacag ctgcggcggg
     1561 cccgacag
//
LOCUS       DASVII010000669         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_283188,
            whole genome shotgun sequence.
ACCESSION   DASVII010000669 DASVII010000000
VERSION     DASVII010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283188"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF309_08675"
     CDS             complement(<1..203)
                     /locus_tag="VF309_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX7466701.1"
                     /translation="MALPPVVERVVLHWGEMGSRWGVNRTVAQIHALLYISPRPLNAE
                     EISDTLAVARSNVSNSLRELQGWR"
     gene            complement(359..>1997)
                     /locus_tag="VF309_08680"
     CDS             complement(359..>1997)
                     /locus_tag="VF309_08680"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX7466702.1"
                     /translation="TRVPRMVWEGDRETVAAYLRGLYQADGNVVSSDDVTTLAIASID
                     MQLIEELQVLWANFGVKSSINVMRGHEQHLLPDGRGGKKSYWSQPLYRLLITSLQGCR
                     IAEEITRLGETRKSEAAARFLRNLQKEGDEQKLHATFTELTRLPNEDAYCLTVDSETH
                     AWTVNGFVTKNTEITLNTNAEEIAVCNLGSVNLLAHMKGGKLDHDKLKRTITTAMRML
                     DNVIDINYYAVRKAHDSNKRHRPVGLGIMGFQDSLHELRIPYASEAAVDFADKSMEAV
                     CYYAYWASTELAKERGRYSTFPGSLWDQGILPHDSLKRLLDERGGYVEVDMSESMDWA
                     ALRKRIKEHGMRNSNCVAIAPTATISNIIGVSASIEPNYQNLFVKSNLSGEFTVVNES
                     MVRDLKKMGLWDAVMVNDLKHFDGSIGKIDRVPQEVRSLYATAFEVEPKWLVEAGARR
                     QKWIDQAQSLNIYMAGASGKKLDETYKLAWLRGLKTTYYLRTLAATSAEKSTGKGGEL
                     NAVSASGGQGAMGAAGDGSADEAKFCSIDNPECEACQ"
BASE COUNT          334 a          642 c          623 g          398 t
ORIGIN      
        1 cgccaaccct gcaattccct gaggctgttg ctgacgttgg aacgcgccac ggccagcgtg
       61 tcgctgatct cctccgcatt gagcggccgg ggcgagatgt aaagcagcgc gtggatctgc
      121 gcgacggtgc ggttcacgcc ccagcggctt cccatctcgc cccagtgcag gacgacgcgc
      181 tcgactacgg ggggaagggc catggatcct cgctaatttc tgtcattaca gaaattacgc
      241 gcaatccccg gggttgtcaa acgggctcgg caaaaaaaga gggaggcagt ttgcctccct
      301 cgcatccctg attcaccttc accccaaacc ctcccttatc gggaggaaaa cacgaacgct
      361 actggcaggc ctcgcactcc ggattgtcga tcgagcagaa cttcgcctcg tcggcagatc
      421 cgtccccggc tgcccccatc gcgccctggc cgcccgacgc ggagacggcg ttcagctcgc
      481 cacccttccc ggtcgacttc tcggcactgg tggccgccag agtacgcaga taataagtcg
      541 ttttcaagcc acgcagccac gcgagcttgt aggtctcgtc gagcttcttg ccggaggcgc
      601 cggccatgta gatgttgagc gactgcgcct ggtcgatcca cttctggcgg cgggcgcccg
      661 cttcgacgag ccacttcggc tcgacctcga aggcggtcgc gtagaggctg cgcacttcct
      721 gcggcacgcg gtcgatcttg ccgatggagc cgtcgaagtg cttgaggtcg ttcaccatca
      781 cggcgtccca gaggcccatc ttcttcaggt cgcgcaccat gctctcgttc accaccgtga
      841 actcgccgga gaggttcgat ttcacgaaga ggttctggta gttgggctcg atggaggccg
      901 acacgccgat gatgttggag atggtcgcgg tcggcgcgat ggccacgcag ttggagttgc
      961 gcatgccgtg ctccttgatc cgcttgcgca gcgccgccca gtccatcgat tccgacatgt
     1021 ccacctcgac atagccgccg cgctcgtcga ggaggcgctt cagcgaatcg tggggcagga
     1081 tgccctggtc ccagagcgaa ccggggaagg tcgagtagcg gccgcgctcc ttcgcgagct
     1141 cggtcgaggc ccagtacgcg tagtagcaca cggcttccat cgacttgtcg gcgaaatcga
     1201 ccgcggcctc gctcgcgtac gggatgcgca gctcatgcag cgaatcctgg aagcccatga
     1261 tgccgaggcc cacggggcga tggcgcttgt tggagtcgtg ggccttgcgc accgcgtagt
     1321 agttgatgtc gatcacgttg tcgagcatgc gcatcgcggt ggtgatggtg cgcttcagct
     1381 tgtcgtggtc gagcttgccg cccttcatgt gggcgaggag attcacgctg ccgaggttgc
     1441 acacggcgat ctcttccgcg ttcgtgttca acgtgatctc ggtgttcttg gtgacgaacc
     1501 cgttcacggt ccacgcatgc gtctcggaat ccaccgtgag gcagtatgcg tcctcgttcg
     1561 gcagccgggt gagctcggtg aaggtagcgt gaagcttctg ctcgtcgcct tccttctgca
     1621 ggttgcgaag aaaacgcgct gcggcctccg acttgcgagt ctcgcccagg cgcgtgattt
     1681 cttccgcgat cctgcaaccc tggagcgagg tgatcaacag gcggtagagc ggctggctcc
     1741 agtaggactt cttcccgccc cggccatccg ggagcagatg ctgctcgtgg ccgcgcatca
     1801 cgttgatcga ggacttgacg ccgaagttgg cccacaacac ctgaagctct tcgatgagct
     1861 gcatgtcgat cgacgcgatc gcaagggtcg tgacgtcatc gctgctcacc acgttgccgt
     1921 cggcctggta cagcccgcgc aaatacgcgg caaccgtctc gcgatcgccc tcccacacca
     1981 tgcgcgggac gcgcgtc
//
LOCUS       DASVII010000670         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_462681,
            whole genome shotgun sequence.
ACCESSION   DASVII010000670 DASVII010000000
VERSION     DASVII010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462681"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF309_08685"
     CDS             <1..243
                     /locus_tag="VF309_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466703.1"
                     /translation="QVAVHARTQLRVAKQPGAGGTFATVETLDAQERIAEVARMLGGL
                     KITEATRRHAQEMLQNARAAGTPLRKEKGKAAAGPG"
     gene            267..1499
                     /locus_tag="VF309_08690"
     CDS             267..1499
                     /locus_tag="VF309_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7466704.1"
                     /translation="MESVGGKAGLVLAGGGARAAYQVGVLQALKEMLPDPKVNPFPII
                     CGTSAGAVNAGALAVNADDFGRAVDNLLEVWRHFEPHHVYRSDFPGAMANSARWFGGF
                     LFGAFLKNRRISLLDNRPLEGLLARQLDFSRIERNIKAGALDAVAITCSGYTSGQSCS
                     FFEGADHFEGWQRSQRIGIKTRIALEHLMASSAIPFLFPPHKLNREFFGDGSMRQVAP
                     VSPALHLGADRVVVVGTARIRHDSPERTRGDLYPTLAQIAGHVLNSIFLDSLAVDLER
                     LERINRTISAVPPEMLKKMGLTLHHVDVLVLTPSEPLDAIAVKHVRSLPWTIRFLLRS
                     VGAMRRGGANLASYLLFERGYCNELIVLGYNDTVKRREEVESFLAGGLSSVPGAYART
                     VKFMVPDQAPEPTGKTAA"
     gene            1501..1947
                     /locus_tag="VF309_08695"
     CDS             1501..1947
                     /locus_tag="VF309_08695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7466705.1"
                     /translation="MTRPAPHALRDYPHSRALSTRWRDLDPYGHMNNVVQYELFDTIV
                     NAWLIERGALDIQHSPVIGLVVETGCNYFTTLTYPEPVTAALRVAHVGRSSVRYEVAL
                     FAKGCASAAAQGHFVHVYVDRETRRPVAELPAALRLALEPLASPAP"
BASE COUNT          329 a          730 c          683 g          324 t
ORIGIN      
        1 caggtcgccg tgcatgctcg cacgcagctt cgcgtggcga agcagcccgg cgcgggcggc
       61 acgttcgcga ccgtcgaaac gctcgacgcg caggagcgaa tcgcggaagt cgcgcgcatg
      121 ctcggaggcc tcaagatcac cgaggcgacc cgccgccatg cgcaggagat gttgcaaaac
      181 gcccgcgctg caggtacacc cttacgcaag gaaaaaggca aagcagcggc cgggccggga
      241 tgagcctgcc gcgcgagggg agaagcatgg aatcggtggg cgggaaggcg ggtctcgtgc
      301 tcgcgggcgg cggcgcccgc gccgcctacc aggtcggcgt gctgcaggcg ttgaaggaga
      361 tgcttcccga cccgaaggtg aacccgttcc cgatcatctg cgggacttcg gcgggggcgg
      421 tgaacgcggg tgctctcgcg gtgaacgccg acgacttcgg ccgcgccgtc gacaacctcc
      481 tcgaggtgtg gcgccatttc gagccacacc acgtctaccg ttccgatttc ccgggcgcga
      541 tggccaacag cgcgcgctgg ttcggcggat tcctcttcgg ggccttcctc aagaaccggc
      601 gcatctcgct gctcgacaac cgtccgctcg agggcctcct cgcacgccag ctcgatttct
      661 cgcgcatcga gcgcaacatc aaggccggcg cgctcgacgc ggtggcgatc acgtgttcgg
      721 gctacacgtc gggacagagc tgcagcttct tcgagggcgc cgatcacttc gagggatggc
      781 agcgctcgca gcggatcggc atcaagacgc gcatcgccct cgagcacctc atggcctcga
      841 gcgcgattcc gttccttttt ccaccgcaca agctgaaccg cgagttcttc ggcgacggct
      901 cgatgcgcca ggtcgcgccg gtgagcccgg cgctgcacct gggtgccgac cgtgtcgtgg
      961 tggtcggcac cgcgcgcatt cgtcacgact cgcccgagcg cacgcgcggc gatctctatc
     1021 ccacgctcgc gcagatcgcg ggccacgtgc tgaactcgat cttcctcgac agcctcgcgg
     1081 tcgacctcga gcgcctggag cgaatcaacc gcaccatcag cgccgtgccc ccggagatgc
     1141 tgaagaagat gggcctcacg ctccaccatg tcgatgtcct ggtgctcacc ccgtcggagc
     1201 ccctcgacgc gatcgcggtg aagcacgtgc gcagccttcc gtggacgatc cgcttcctcc
     1261 tgcgaagcgt gggcgcgatg cgccgcggcg gggcgaacct cgcgagctat ctcctcttcg
     1321 agcgcggcta ctgcaacgag ctcatcgtgc tcggttacaa cgacacggtg aagcgccgcg
     1381 aggaggtcga gtccttcctc gccggcggtc tttcgtccgt gcccggcgcc tacgctcgca
     1441 ccgtgaagtt catggtgccg gaccaggcgc cggagcccac cgggaagacc gcggcatagc
     1501 atgacgcgcc cggccccgca cgcccttcgc gactatccgc actcccgcgc gctcagcacg
     1561 cgctggaggg acctcgatcc ttacggccac atgaacaacg tcgtgcagta cgagctcttc
     1621 gatacgatcg taaacgcgtg gctgatcgag cgcggcgcgc tggacatcca gcacagcccc
     1681 gtgatcgggc tcgtggtcga gacgggctgc aactacttca cgacgctcac ctacccggag
     1741 ccggtgacgg cggcgctgcg cgtcgcgcac gtcggccggt cgagcgtgcg ctacgaggtt
     1801 gcgctctttg cgaagggctg cgcgagcgcc gccgcgcagg gtcacttcgt gcacgtctac
     1861 gtcgaccgcg agacccgccg tcccgtagcc gagcttcccg cggcgctgcg cctcgccctc
     1921 gagcccctcg cgtcgcccgc accctagggc agcaggatcg aggagcgctt gtccggcgcg
     1981 gcgccgaaga tgcccgcgag gaatgaagcg agatcctgca cttcctcggc gctcacggag
     2041 tgcggcatcg gatacgcgtg ccattg
//
LOCUS       DASVII010000671         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_571064,
            whole genome shotgun sequence.
ACCESSION   DASVII010000671 DASVII010000000
VERSION     DASVII010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571064"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1533
                     /gene="kdpA"
                     /locus_tag="VF309_08700"
     CDS             <1..>1533
                     /gene="kdpA"
                     /locus_tag="VF309_08700"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX7466706.1"
                     /translation="SGVDPAHEMRWTEYAIAMLVFNFLGFLAVYALQRFQAVLPLNPQ
                     AFGAVSPDSSFNTAVSFITNTNWQGYGGESTMSYLTQMLALTVQNFVSAASGMATLVA
                     FARGFSRKSAQTIGNFWVDMTRSTLYILLPLSLVLAVALVSQGMPRTFSAYPTVPTVE
                     ATEYDNPKLDQAGQPVKDDKGNPVTEKATMKEQQIAVGPVASQVAIKQLGTNGGGFFN
                     VNSAHPFENPTPLANFLEVLSILVISAALCYTFGVLVADTRQGWAVLATMTILFAVML
                     AVCVGAEQSGNPALTKAGADQVASATQPGGNMEGKELRFGITNSALWATATTAASNGS
                     VNAMHDSFTPLGGLVPMWLIQFQEVIFGGVGSGLYGMLIFAIIAVFVAGLMIGRTPEY
                     LGKKIESFEMKMASVTILIPPLVVLVGTAIAVMVDPGKAGVANPGAHGFSEILYAFSS
                     AGNNNGSAFAGLSANTVFYNTALGIAMLLARFWLAVPTLAIAGALAAKKSIPPGLGTL
                     PTH"
BASE COUNT          244 a          530 c          489 g          270 t
ORIGIN      
        1 tcgggcgtgg acccggccca cgagatgcgc tggaccgagt acgcgatcgc gatgctggtc
       61 ttcaatttcc tgggcttcct cgcggtgtac gcgctgcagc gcttccaggc ggtcctgccc
      121 ctcaatcccc aggctttcgg cgcggtctcg ccggactcgt cgttcaacac cgcggtgagc
      181 ttcatcacca acaccaactg gcagggctac ggcggcgagt cgacgatgag ctacctcacg
      241 cagatgctcg ccctcaccgt gcagaacttc gtctcggcgg cgagcggcat ggctacgctg
      301 gtcgcgttcg cgcgcggctt ttcgcgcaag agcgcgcaaa cgatcggcaa cttctgggtc
      361 gacatgacgc gcagcacgct ctacatcctg ctgcccctgt cgctcgtcct tgccgtcgcg
      421 ttggtgagcc agggcatgcc gcggaccttc tcggcatacc ccacggtgcc gaccgtcgaa
      481 gcgaccgagt acgacaatcc caagctcgat caagccgggc agccggtgaa ggacgacaag
      541 ggaaatcccg tcaccgagaa ggccacgatg aaggagcagc agatcgcggt gggtcccgtg
      601 gcttcgcagg tcgctatcaa gcagctgggc accaacggcg gcggcttctt caacgtgaac
      661 tccgcgcacc ccttcgagaa tccgacgccg ctcgcgaact tcctcgaggt gctctcgatt
      721 ctcgtgatct ccgcggcgct ttgctacacg ttcggcgtgc tcgtggccga cacgcgccag
      781 ggctgggcgg tgctcgcgac gatgacgatc ctcttcgccg tgatgctcgc cgtgtgcgtg
      841 ggcgccgagc agtccgggaa tcccgcgctc acgaaggcgg gcgccgacca ggtcgcctcg
      901 gcgacgcagc ccggcggcaa catggaagga aaggaactgc gcttcggcat caccaattcg
      961 gcgttgtggg cgaccgcgac gaccgcggcc tccaacggct cggtgaacgc gatgcacgac
     1021 tccttcacgc cgctgggcgg cctggtgccg atgtggctca tccagttcca ggaagtcatc
     1081 ttcgggggcg tgggctcggg cctctacggc atgctgatct tcgcgatcat cgcggtcttc
     1141 gtggccggcc tcatgatcgg gcgcacgccc gagtacctcg gcaagaagat cgagtccttc
     1201 gagatgaaga tggcctcggt cacgatcctc atcccgccgc tggtcgtgct cgtgggaacg
     1261 gccattgccg tgatggtgga cccgggcaag gcgggtgtcg ccaacccggg tgcccacggg
     1321 ttctccgaga tcctctacgc gttctcctcg gccggcaaca acaacggcag cgccttcgcg
     1381 ggcctgtcgg ccaacacggt gttctacaac acggcgctcg gcattgcgat gctcctcgcg
     1441 cgcttctggc tcgccgtgcc gacgctcgcg atcgcggggg cgctggccgc caagaagagc
     1501 atcccgccgg gcctcggcac tctccccacc cac
//
LOCUS       DASVII010000672         4209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_462774,
            whole genome shotgun sequence.
ACCESSION   DASVII010000672 DASVII010000000
VERSION     DASVII010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462774"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VF309_08705"
     CDS             complement(<1..765)
                     /locus_tag="VF309_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX7466707.1"
                     /translation="MPVTLSGLNIYPVKGLKGIALTESRCTDRGLEHDRRWMVVDSEG
                     VFLTQRDHPRMATVWTDIAGDELVLSAPDVGEVAVPLDPAMAPPLRVRVWNSDCAALA
                     VSTAADAWLSAYLGMGCRLVYMPDESKRASNPQYAGYERFVSFADGYAYLAIGEASLA
                     DLNARLVAKNHPALPMNRFRPNLVVSGTEPYAEDRWGDIRVGDAVLRGVKPCGRCQVT
                     TTDQATGEVRGPEPLATLSEYRESSEFGTMFGMNLVT"
     gene            850..2154
                     /locus_tag="VF309_08710"
     CDS             850..2154
                     /locus_tag="VF309_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7466708.1"
                     /translation="MALWDKLIGELVDIVEWLDDSKDTLAYRFERYQNEIKYGAKLVV
                     REGQMAAFINEGKLADVYKPGTYELTTQNMPILATLRGWKYGFNSPFKAEAYFISTRK
                     FTDLKWGTPGPAMMRDKDFGMVRVTSFGIYSVRAKDAGIIIRDLVGTEGRFTTEDIQE
                     NLRGKIGLRIKELMPELGIPVIEMTAKVFEMGNKLRERLGPDFEALGLELTEVQVQNI
                     GLPEEVEKAIDKRGAIAAVGNLQAYTQYETASSIHDAASNPGGAAGVGMGLGAGIAMG
                     GQMMNNMSGAASATAMPPPIPGAVAFFIAAVGNLQAYTQYETASSIHDAASNPGGAAG
                     VGMGLGAGIAMGGQMMSNMSGAAGATAMPPPIPGAIAFFVAAGQNQAGPFDLGALQQQ
                     VASGALTRNSLVWKNGMAQWVKAGEVPDLAPLFANVPPPIPT"
     gene            2151..3305
                     /locus_tag="VF309_08715"
     CDS             2151..3305
                     /locus_tag="VF309_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466709.1"
                     /translation="MSDVPAQPGAASDPGPRQDDGPAQFIQTFPCTGCGAKLSFAPGT
                     RNLKCEFCGKQNEIAENDARIEELDFDTYLKALEGKQETFEEEHVKCDKCGAEQNLPA
                     NVFAAVCTFCGASIVSKSYANRRIKPKAMVPFAIDRRHAQDAFRKWVKGLWLAPGELK
                     KYAQSDAGVTGTYVPFWTYDCHTSTDYRGQRGENYYTTETYTTRNSSGESVTQTRQVK
                     QTNWTPVSGHVDFFHDDVLVMASTSLPREVRGATAQWNLKALVPYQSEFVSGFRAEAY
                     QVGLREGYPLAKEQIDSHVYGLVRGDIGGDEQRVDDIATRYSDIKFKHVLLPVWISAY
                     RFRNKTYRFLVNGQTGEVAGESPLSWQRVTLLVIGIIVVVVIALVLSGRH"
     gene            complement(3367..3870)
                     /locus_tag="VF309_08720"
     CDS             complement(3367..3870)
                     /locus_tag="VF309_08720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7466710.1"
                     /translation="MTIKAGDRLPEGNVQEFIEVEGNGCTVGPNTFKVQDLVKGKKIA
                     IFGLPGAFTPTCSAKHVPSYLNNMDKLKAKGVDEVWCFAVNDPFVMGAWSRDQKAGGK
                     IRFMADGSADYTKKLGLELDLTARGLGMRCNRFSMLVDNGVVKTLNVEGPGKFEVSDG
                     DTMVKQA"
     gene            complement(3904..>4209)
                     /locus_tag="VF309_08725"
     CDS             complement(3904..>4209)
                     /locus_tag="VF309_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX7466711.1"
                     /translation="FMRGFWVNATNPKGIIFFAAILPQFIDVALPQATQYAILAGTTF
                     AVDLVIMMGYTALAAKVLRVMSDPRHLRWVNRGIGGAFVAAGVALASFRRAATLAAT"
BASE COUNT          760 a         1357 c         1411 g          681 t
ORIGIN      
        1 ggtcacgagg ttcatcccga acatcgtgcc gaactcgctg ctttcgcggt attccgagag
       61 ggtggcgagc ggctcggggc cgcgcacttc gcccgtcgcc tggtcggtgg tcgtgacctg
      121 gcatcgtccg cagggcttca cgccgcgcag caccgcgtcg cccacgcgga tatcgcccca
      181 tcgatcttcg gcatacggct cggttccgga cacgacgagg ttcgggcgga agcggttcat
      241 cggcagcgcc gggtgattct tcgccaccag gcgcgcgttg agatcggcaa gagaggcctc
      301 gccgatcgcg agataggcgt agccgtcggc gaagctcacg aagcgctcat agcccgcgta
      361 ctgggggttc gacgcgcgct tcgattcgtc gggcatgtag acgaggcggc agcccattcc
      421 cagataggcg gagagccacg catcggccgc ggtggagacg gcgagcgcgg cgcagtcgct
      481 attccagacg cgcacgcgca gcggcggggc catcgcggga tcgaggggca cggcgacttc
      541 tcccacgtcg ggcgccgaga gcacgagctc atccccggcg atgtcggtcc agacggtcgc
      601 catgcgcgga tggtcgcgtt gcgtgaggaa tacgccctcg gagtcgacca ccatccagcg
      661 ccggtcgtgc tcgaggcccc ggtcggtgca gcgcgactcg gtgagcgcga tgcccttcag
      721 gcccttcacg ggatagatgt tgaggccgga gagggtcacg ggcatgggga atccggaata
      781 tggtgaaatg cgtctaagat aattggaaaa attagtaccg ggtacggatt atcactgaaa
      841 ggggaaacca tggctctctg ggacaagctg ataggcgaac tggtcgatat cgtcgaatgg
      901 ctcgacgact cgaaggacac gctcgcatac cgcttcgagc gctaccagaa cgagatcaag
      961 tacggcgcca agctcgtcgt gcgcgaaggc cagatggccg ccttcatcaa cgagggcaag
     1021 cttgccgacg tctacaagcc cggcacctac gagctcacca cgcagaacat gccgatcctg
     1081 gcgacgctgc gcggctggaa gtacggcttc aacagcccgt tcaaggccga ggcctacttc
     1141 atctcgacgc gcaagttcac cgacctgaaa tggggcacgc ccggccccgc gatgatgcgc
     1201 gacaaggatt tcggcatggt ccgcgtgacc tcgttcggca tctactcggt gcgcgccaag
     1261 gatgcgggaa tcatcatccg cgacctggtc ggcaccgagg ggcgtttcac caccgaggac
     1321 atccaggaga acctgcgcgg caagatcggg ctgcgcatca aggagctcat gccggagctc
     1381 ggcattccgg tgatcgagat gacggccaag gtcttcgaga tgggcaacaa gcttcgcgag
     1441 cgcctcgggc ccgacttcga ggcgctcggc ctcgagctga ccgaggtgca ggtgcagaac
     1501 atcggcctgc cggaggaagt cgagaaggcg atcgacaagc gcggcgcgat cgccgccgtc
     1561 ggcaacctgc aagcctatac gcagtacgag accgcctcct cgatccacga tgccgcgagc
     1621 aatccgggcg gcgcggcggg cgtcggcatg ggactcggcg ccggtatcgc gatgggcggg
     1681 cagatgatga acaacatgtc gggcgccgcg agcgcgaccg cgatgccgcc gcccattccc
     1741 ggcgccgtcg cattcttcat cgccgcggtc ggcaacctgc aggcctatac gcagtacgag
     1801 accgcctcct cgatccatga cgccgcgagc aatccgggcg gggccgcggg cgtgggcatg
     1861 ggcctcggcg cgggaatcgc gatgggcggg cagatgatga gcaacatgtc gggcgccgcg
     1921 ggcgcgaccg cgatgccgcc gcccattccg ggcgccatcg cgttcttcgt cgccgcgggg
     1981 cagaaccagg cggggccgtt cgatctcggc gcgctccagc agcaggtcgc ctccggagcg
     2041 ctcacgcgca actcgctcgt gtggaagaac ggcatggcgc aatgggtgaa ggcgggcgaa
     2101 gtcccggatc tcgctcccct cttcgcgaac gttcctccgc cgattccgac gtgagcgacg
     2161 tcccggcgca gcccggggcc gcatcggatc ccggcccgcg ccaggatgac ggcccggcgc
     2221 agttcatcca gaccttcccg tgcacgggct gcggcgcgaa gctgtcgttc gccccgggga
     2281 ctcgcaacct caagtgcgag ttctgcggca agcagaacga gatcgcggag aacgacgcgc
     2341 gcatcgagga gctcgacttc gacacctacc tgaaggccct cgagggcaag caggagacgt
     2401 tcgaggaaga gcacgtgaag tgcgacaagt gcggcgcgga gcagaacctc cccgccaatg
     2461 tgttcgccgc ggtgtgcacg ttctgcggcg cgagcatcgt gtcgaagagc tacgcgaacc
     2521 gccgcatcaa gcccaaggcg atggtgccct tcgcgatcga ccggcgccac gcccaggacg
     2581 cattccgcaa atgggtgaag ggcctgtggc tcgcccccgg cgagctgaag aaatacgcgc
     2641 agagcgacgc gggcgtcacc ggaacctacg tgccgttctg gacctacgac tgccatacct
     2701 ctaccgacta tcgcgggcag cgcggcgaga actactacac gaccgagacg tacacgacgc
     2761 gcaactcctc cggcgaatcg gtcacccaga cgcggcaggt aaagcagacc aactggacgc
     2821 ccgtgtcggg gcacgtggat ttcttccacg acgacgtgct cgtgatggca tcgacttccc
     2881 tgccccgcga ggtacggggc gcgaccgcgc aatggaacct gaaggcgctc gttccctacc
     2941 agtccgagtt cgtgagcggc ttccgcgccg aggcctacca ggtcgggctg cgcgagggct
     3001 atccgctcgc gaaggaacag atcgattcgc acgtgtacgg actggtgcgc ggcgacatcg
     3061 gcggcgacga gcagcgcgtc gacgacatcg ccacgcgcta cagcgacatc aagttcaagc
     3121 acgtgctgct gccggtgtgg atctcggcct atcgcttccg caacaagacc taccgcttcc
     3181 tcgtgaacgg ccagacgggg gaagtcgccg gcgagagccc gctgtcgtgg cagcgggtga
     3241 ccctcctcgt gatcggcatc atcgtggtgg tagtgatcgc gctcgtgttg agcggccggc
     3301 actgaatggg tgtcaggtac gaatttccga attcgtacct gacacccatt tcccgggggc
     3361 gatcgatcag gcctgcttca ccatcgtgtc gccgtcggag acctcgaact tgccgggccc
     3421 ttcgacgttg agcgtcttca cgacgccgtt gtcgacgagc atcgagaagc ggttgcagcg
     3481 catgccgagg ccgcgcgcgg tcaggtcgag ctcgaggccg agcttcttcg tgtagtcggc
     3541 actgccgtcg gccatgaagc ggatcttgcc gccggccttc tggtcgcgcg accaggcgcc
     3601 catcacgaag ggatcgttca ccgcgaagca ccacacctcg tcgacgccct tcgccttcag
     3661 cttgtccatg ttgttgaggt agctcggcac gtgcttcgcg gagcacgtgg gcgtgaaggc
     3721 gccgggcagg ccgaagatcg cgatcttctt gcccttcacg aggtcctgca ccttgaaggt
     3781 gttggggccc acggtgcagc cgtttccttc gacttcgatg aattcctgca cgttgccttc
     3841 gggaaggcgg tctccggcct tgatcgtcat gttcttctcc tgggttgagt gggaattgcc
     3901 aaatcaggtg gcggcgaggg tcgccgcgcg ccgaaagctc gccagcgcca cgccggccgc
     3961 gacgaacgcg ccgccgatgc cgcggttcac ccagcgcagg tggcgcggat cgctcatcac
     4021 tcgcagcacc ttcgcggcga gcgccgtgta gcccatcatg atcacgaggt ccacggcgaa
     4081 ggtcgtcccc gcgagaatcg cgtattgcgt ggcttgcggg agcgcgacgt cgatgaactg
     4141 cggaaggatc gccgcgaaaa agatgatgcc cttggggttg gtcgcgttca cccagaagcc
     4201 gcgcatgaa
//
LOCUS       DASVII010000673         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_391321,
            whole genome shotgun sequence.
ACCESSION   DASVII010000673 DASVII010000000
VERSION     DASVII010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391321"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF309_08730"
     CDS             complement(<1..382)
                     /locus_tag="VF309_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7466712.1"
                     /translation="MSAALAVRDLRKSFGGLPAADGVTLEVGEGERHLIIGPNGAGKT
                     TLFNLISGDLRPDSGSVRLFGEEVAGLRPDQRARRGMARTYQILTLFPHDTLAHNIVL
                     SLLGMSPSRWRMLAPLAGMGELHDR"
     gene            complement(379..1323)
                     /locus_tag="VF309_08735"
     CDS             complement(379..1323)
                     /locus_tag="VF309_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7466713.1"
                     /translation="MKHAARAAGIAIVLAAFPLAIGGEYYVNLASQVLIAAIFAMSLN
                     LLVGYGGMTSLGHAAYLGVAAYATAWLMLRSGLGPLAAGGAALVITTLLAAFYGVLSL
                     RASGLSFLMITLALGQILWGLAYRWVNVTGGDNGLSVLARPAPFGIDLTGATAFYYFS
                     LVVFAIAFAVITLLVRSPFGASLRATRDQARRMSALGHNVWLVRWTAFVLAGFWGAVS
                     GVLYMYYNNYISPHALSLTQSAEVLLMVIAGGPSTILGPVVGAILVVLMKNVASAYVE
                     HWTLLLGVVFVVIVMFLPDGIVPGIMRLYQRSRRGSDR"
     gene            complement(1320..>1597)
                     /locus_tag="VF309_08740"
     CDS             complement(1320..>1597)
                     /locus_tag="VF309_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466714.1"
                     /translation="AGLAGMGGVIAGPILSVYPGLDADMLPLALVVVILGGVGSIPGA
                     FVGSIVIGFIYTFGQALLPDLAYVILFLPMVIVLVARPRGLFGRVAA"
BASE COUNT          299 a          566 c          543 g          189 t
ORIGIN      
        1 ccctgtcgtg gagctccccc attccggcga gcggcgcgag catccgccag cgcgaaggcg
       61 acatcccgag caggctcagc acgatgttgt gcgcgagcgt gtcgtgcgga aagagcgtga
      121 ggatctggta ggtgcgcgcc atgccgcgcc gcgcgcgttg atcggggcga agcccggcga
      181 cctcctcgcc gaagagccgc accgatcccg agtcggggcg caggtccccg ctgatcaaat
      241 tgaagagcgt ggtcttgccc gcgccgttgg ggccgatgat gaggtggcgc tcgccctcgc
      301 ccacctcgag cgtcacgccg tcggccgcgg gcaatccgcc gaaggacttg cgcaggtccc
      361 gcaccgccag ggccgcgctc atcgatcgct cccgcgccgc gaacgttggt agagccgcat
      421 gatccccggc acgatcccgt cgggaaggaa catcacgatc accacgaaca ccacgcccag
      481 cagaagcgtc caatgctcga cataggcgct cgccacattc ttcatgagga ccacgaggat
      541 cgcgcccacc accggcccga gaatcgtcga gggccctccg gcgatcacca tgaggagcac
      601 ttcggccgac tgcgtgagcg acagcgcgtg cgggctgatg tagttgttgt agtacatgta
      661 gagcacgccc gagaccgcgc cccagaagcc cgcgagcacg aacgcggtcc agcggacgag
      721 ccacacgttg tgccccagcg cgctcatgcg ccgcgcctgg tcgcgcgtcg cgcgcaggct
      781 cgcgccgaag ggcgagcgca ccagcagcgt gatgacggcg aaggcgatcg cgaacaccac
      841 gagcgagaag tagtagaacg cggtcgcgcc cgtcaggtcg atgccgaacg gcgccggacg
      901 cgcgagcacc gacagcccgt tgtcgccgcc ggtcacgttg acccatcggt acgcgagccc
      961 ccacaggatc tggccgagtg ccagcgtgat catgaggaac gagagaccgc tcgcgcgcag
     1021 gctgagcacc ccgtagaacg ccgcgaggag cgtcgtgatg acgagcgccg ccccgcccgc
     1081 ggcgagcggc ccgagccccg agcgcagcat gagccacgcg gtggcgtagg cggcgacgcc
     1141 gaggtaggcc gcatggccga gggaagtcat gccgccatag cccaccagca ggttcaggct
     1201 catcgcgaag atcgccgcga tgagcacctg gctcgcgaga ttcacgtagt actcgccgcc
     1261 gatcgccagc gggaatgcgg cgagcacgat cgcgatgccc gccgcccggg cggcgtgctt
     1321 cacgcggcga cccggccgaa gagcccgcgc gggcgcgcga cgagcacgat caccatcggc
     1381 aggaagagga tcacgtacgc gagatcgggc agcagcgcct ggccgaaggt gtagatgaac
     1441 ccgatcacga tgcttcccac gaacgcgccg ggaatgctgc ccacgcctcc gaggatcacc
     1501 accacgaggg cgagcggcag catgtccgca tcgagcccgg gatacaccga caggatcggc
     1561 cccgcgatga cgccgcccat tcccgcaagg cccgcgc
//
LOCUS       DASVII010000674         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_318743,
            whole genome shotgun sequence.
ACCESSION   DASVII010000674 DASVII010000000
VERSION     DASVII010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318743"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VF309_08745"
     CDS             complement(<1..368)
                     /locus_tag="VF309_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466715.1"
                     /translation="MKTLMSWSTGKDSAWSLYVLRQTPGIEVAGLFTTVNAAFDRVAM
                     HAVRRALLEAQAAAAGMPLHVIEIPWPCPNEAYEVRMGAFVAEQTARGVEAMAFGDLF
                     LEDIRAYREAKLANSGIAPL"
     gene            416..1123
                     /locus_tag="VF309_08750"
     CDS             416..1123
                     /locus_tag="VF309_08750"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX7466716.1"
                     /translation="MTDFRHIVILTGAGLSAESGLATFRDKDGIWAKYDYREVATPEG
                     FRANPQLVHDFYNQRRRGLAGVTPNAAHFALARLEREHKGSVTVVTQNIDPLHEAAGS
                     RDSIHMHGELLKALCALCGTRHAWRNDLGLETQCPGCQEKGSMRPDVVWFGEMPYAME
                     RIYAALAEADLFISIGTSGNVYPAAGFVAVARENGAHTVELNLEPSDGANLFQEAIHG
                     PATQVVPAYVERLLVDG"
     gene            complement(1188..>1616)
                     /locus_tag="VF309_08755"
     CDS             complement(1188..>1616)
                     /locus_tag="VF309_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HEX7466717.1"
                     /translation="RVADLFCGLGTFTFPLAKRSAVLAVDGDKQAIEALANAAKRTPG
                     LKPIETKSRDLFREPLSGKELESFDAVVFDPPRAGAAAQASALAASKVQTVVAVSCNP
                     ATLARDARILIDGGFTLEAVTPIDQFLFSPHIEAVAVLRR"
BASE COUNT          281 a          557 c          523 g          255 t
ORIGIN      
        1 aacagcggcg cgatgcccga gtttgcgagc ttcgcctcgc gataggcacg gatatcctcc
       61 aggaacagat cgccgaaggc catggcctcg acaccgcgcg ccgtctgctc cgccacaaat
      121 gcgcccatgc gcacctcgta ggcctcgttg gggcacggcc acggtatctc gatgacatgc
      181 agcggcatcc cggcggcggc ggcctgcgct tccagcagcg cgcgccgcac ggcatgcatg
      241 gcgacgcggt cgaaggcggc attgacggtg gtgaacagcc ccgcgacctc aatcccaggc
      301 gtctgccgca gcacatagag cgaccacgcc gaatccttgc ccgtgctcca gctcatcagc
      361 gtcttcatgc attcctcgcg ttgcgctcgt ccacggcggc ggctatcgct cgacgatgac
      421 agacttccgg cacatcgtca tcctcacagg cgccggcctc tccgccgagt cggggctcgc
      481 gaccttccgc gacaaggacg gcatctgggc caagtacgac taccgtgaag tggccacgcc
      541 cgagggcttc cgcgccaacc cgcagctcgt gcacgatttc tacaatcagc ggcggcgcgg
      601 gctcgccggc gtgaccccga acgccgcgca cttcgccctc gcgcggctgg agcgcgagca
      661 taaaggctca gtcaccgtcg tcacgcagaa catcgatccc ctgcacgagg ccgcgggctc
      721 gcgcgactcg atccacatgc acggggagct cttgaaagcg ctgtgcgcgc tatgcggcac
      781 gcgccatgcc tggcgcaacg acttgggctt ggagacgcaa tgccccggct gccaggagaa
      841 aggcagcatg cgccccgacg tcgtgtggtt cggcgagatg ccctacgcga tggagcgcat
      901 ctacgcggct ctcgctgaag ccgatctctt catttccatc ggcaccagcg gcaacgtcta
      961 cccggcagcc ggcttcgtgg ccgtggcccg ggagaacggc gcgcatacag tggagctcaa
     1021 tctcgagccc tcggatggcg cgaacctgtt ccaagaggcg atccacggcc ccgcgacgca
     1081 agtcgtcccc gcctacgtcg agcggctgct tgtggatggc tgatcggcgc gagccttcac
     1141 ctcccccata gggagaggtg aggggagcgt actttggctt cgaagctcta ccgtcgcagc
     1201 acggcgacgg cctcgatgtg cggggagaac agaaactggt cgatcggcgt caccgcctcg
     1261 agcgtgaagc cgccgtcgat caggatgcgc gcatcgcgcg cgagcgtcgc cgggttgcag
     1321 gagacggcga caaccgtctg aaccttggac gccgcgagcg cggaggcctg tgcggccgca
     1381 cctgcgcgcg ggggatcgaa aaccacggcg tcgaagctct cgagctcctt gcctgacagc
     1441 ggctcgcgga agaggtcacg cgacttggtc tcgatcggtt tcaagcccgg tgtgcgcttg
     1501 gccgcatttg ccaacgcttc gattgcctgc ttgtccccat cgacggcgag cacggctgat
     1561 cgcttcgcca gcgggaaggt gaaggtgccg agcccgcaaa agagatcggc gacacg
//
LOCUS       DASVII010000675         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_32193, whole
            genome shotgun sequence.
ACCESSION   DASVII010000675 DASVII010000000
VERSION     DASVII010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32193"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VF309_08760"
     CDS             complement(<1..230)
                     /locus_tag="VF309_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7466718.1"
                     /translation="MKRTDKEQLVRELKEQIGSAKALYYTDFTGLNVKRMTELRRKFR
                     KAGVQYVVIKNTLALRAVNESGLVGERLKGPTG"
     gene            complement(227..925)
                     /gene="rplA"
                     /locus_tag="VF309_08765"
     CDS             complement(227..925)
                     /gene="rplA"
                     /locus_tag="VF309_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7466719.1"
                     /translation="MKTHGKKYNAAAKNREFGQAYQAKQALDIVKKSAFAKFDETVEA
                     VVRLGVDPRHADQVVRGTVVLPEGTGSTVRVLVIAVGDKAREAEAAGADYVGVEFLQK
                     IKDGWLDFDVLIATPDQMGQLGQLGRVLGPRGLMPNPKAGTVTFNVGQAVKETKGGKI
                     EFRVDKGGNVHVPLGKVSFALEALESNFTAFMEQIIRSKPSAAKGVYVRNVSVSSTMG
                     PGVAVDTTPYRLRA"
     gene            complement(926..1348)
                     /gene="rplK"
                     /locus_tag="VF309_08770"
     CDS             complement(926..1348)
                     /gene="rplK"
                     /locus_tag="VF309_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7466720.1"
                     /translation="MAKKVSGFVKLQIPAGKANPAPPVGTALGPQGINIMAFCKEFNA
                     RTQGQDTILPVEITIFTDKSFTFILKTPPAAILVKKAIGLEKGSGQPNRNKVGKISKA
                     QIKQIAELKMPDLNTDSIESAMAMIAGAARSMGVEVVD"
BASE COUNT          233 a          508 c          435 g          330 t
ORIGIN      
        1 cccgtcggac ccttcagccg ctcgccgacg agtcccgact cgttcacggc gcgcagcgcg
       61 agggtgttct tgatgacgac atactgcaca ccggccttcc tgaacttccg gcgcagctcc
      121 gtcatccgct tcacgttcaa ccccgtgaag tccgtatagt acagcgcctt ggcgctgcca
      181 atttgctctt tcagctcgcg aacgagctgc tccttgtcgg ttctcttcat gcgcgtagcc
      241 ggtagggagt ggtgtcgacg gcaacgccgg ggcccatcgt gctggacacc gacacgttgc
      301 ggacgtagac gcccttggcc gcactcggct tcgagcggat gatctgttcc atgaacgccg
      361 tgaagttgga ctccaacgcc tcgagcgcga acgacacctt gccgagcggg acgtgcacat
      421 tgccgccctt gtcgacacgg aactcgatct taccgccctt ggtctccttc accgcctgac
      481 cgacattgaa cgttaccgtt cccgccttgg ggttcggcat caggccgcgc ggaccgagca
      541 ctcggccaag ctggccgagc tgccccatct gatccggcgt cgcgatgagc acgtcgaaat
      601 cgagccagcc gtccttgatc ttctgcagga actcgacgcc gacgtagtcc gcgcccgccg
      661 cttccgcttc gcgcgcctta tcgccgacgg cgatgacgag cacgcgaacg gtcgagccgg
      721 taccctcagg gagcacgacc gtgccgcgga ccacctggtc cgcatgccgt gggtcgacgc
      781 cgaggcgaac caccgcctcc accgtctcat cgaacttggc gaacgccgac ttcttcacga
      841 tgtcgagcgc ctgcttggcc tgatacgcct ggccgaactc gcggttcttc gccgcggcgt
      901 tgtacttctt gccgtgtgtc ttcatttagt cgacgacctc cacacccatc gaccgcgccg
      961 cgccggcgat catcgccatc gcggactcga tcgaatcggt gttcaggtcg ggcatcttga
     1021 gctcggcgat ctgcttgatc tgcgccttgc tgatcttgcc caccttgttg cggttcggct
     1081 gtcccgatcc cttctcgagg ccgatcgcct tcttcaccag gatcgccgcg ggcggcgtct
     1141 tgaggatgaa ggtgaacgac ttgtcggtga agatcgtgat ctcgacgggc aggatcgtat
     1201 cctggccctg cgtccgagcg ttgaactctt tgcagaaggc catgatgttg atgccctgcg
     1261 ggccaagcgc cgtacccacc ggaggagccg gattggcctt ccccgccgga atttggagct
     1321 tcacgaaacc cgatactttt tttgccatgc gctctccctt tgcgtagccc gcgtgaattc
     1381 acgggtcgta ctgccactgt tgattttcgt cgctgtggtc ggcgacggac tgctcactct
     1441 ttactcgtgc tgcacactca tcgcgctgac tgggtctgat ggtcggatag tctgatggtc
     1501 tccggc
//
LOCUS       DASVII010000676         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_678867,
            whole genome shotgun sequence.
ACCESSION   DASVII010000676 DASVII010000000
VERSION     DASVII010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678867"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..757
                     /gene="mraZ"
                     /locus_tag="VF309_08775"
     CDS             308..757
                     /gene="mraZ"
                     /locus_tag="VF309_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7466721.1"
                     /translation="MFRGHFEHAIDAKGRTSLPSRFRDVLASTGDVRAVLTPSPFDPC
                     LHLFPMKAWEEFETKIGALPQFDTNVIRLRRLYVSAAVECEIDKAGRVLVPNSLREHA
                     MLQKDVLWAGMGQTVELWAREKWNEVQAMTANERDSFRRAVAEQLRI"
     gene            760..1671
                     /gene="rsmH"
                     /locus_tag="VF309_08780"
     CDS             760..1671
                     /gene="rsmH"
                     /locus_tag="VF309_08780"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7466722.1"
                     /translation="MAPDQFLPGPSPVTFGHTTVLIAETLAALAPRPGGVYVDATLGG
                     GGHAEAILEAAPGATLVGIDQDPRAIEAARQRLDRFGASVKLVVGRFSELETHLGDFV
                     GVDGIIADLGVSSPQIDDPSRGMSFRSEGPIDMRMGDTEGETALDLIERVGSEELADI
                     IYQYGEERRSRRIARCIHQAYRAGELRTTLDLRRAVVRAVGPARVGGVDPATRTFQAL
                     RIAVNRELEELKALLEVAPRVLLPSAPLVIISFHSLEDRLVKRAFLESATWERMTPKP
                     VVPSRVETEQNPRSRSAKLRAARKVAS"
     gene            1764..>1945
                     /locus_tag="VF309_08785"
     CDS             1764..>1945
                     /locus_tag="VF309_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466723.1"
                     /translation="MELGYDLGKSRGEQARLREVKRVLELEAASYETPQRVEVVARTL
                     LGMQPPAADRIIPVTGL"
BASE COUNT          340 a          605 c          657 g          343 t
ORIGIN      
        1 gatgatgacc gcgagcggga gcaagagtcg aaagggtccg ttcaatgggt cgtcgctcgt
       61 ccaggcacgg tgtcattcaa gcgtcaaagg gcgcgcacaa gtcgttggaa caaatagcac
      121 ccccgggcag gcaaccgaag atgggggtac ataattatac tgatactgca atcatgcaga
      181 tcgcgtgggg tgcgcgcaaa ctattgacac cccctggctt tcccccgtac gttgacccca
      241 gttcgtggga taaagtggca aagagtgcct caccagcccg cggagtggca gccaggaccg
      301 cggaaacatg ttccgaggac atttcgagca tgcaatcgat gcgaagggtc gaaccagcct
      361 tccatctcgg tttcgcgacg tgctcgcgtc gacgggtgac gtgcgcgcgg tgctgacgcc
      421 ttcgccgttc gatccgtgct tgcacctttt tccgatgaag gcgtgggagg agttcgaaac
      481 gaagatcggc gcgctgcccc agttcgatac gaacgtcatc cgcttgcggc ggctctatgt
      541 gtcggcggcc gtggagtgcg agatcgacaa ggcgggccgc gtgctggttc cgaattcact
      601 gcgcgagcac gcgatgctgc aaaaagacgt gctgtgggcg ggcatggggc aaacggtcga
      661 gctctgggcg cgcgaaaaat ggaacgaagt gcaggccatg acggcgaacg agcgcgacag
      721 cttccggcgt gccgtcgcgg agcagctaag gatatgaacg tggcgccgga tcaattcctg
      781 ccgggtcctt cgccggtcac ctttggacat accaccgtcc tcatcgccga gacccttgcg
      841 gcgctcgctc cgcgcccggg cggcgtctac gtcgacgcga ctctgggagg cggcgggcac
      901 gccgaggcaa ttctcgaggc tgcgccgggc gccaccctcg tcggcatcga tcaggatccg
      961 cgcgccatcg aggccgcgcg acagcggctc gatcgtttcg gcgcgtcggt gaagctcgtg
     1021 gtcggccgtt tttcggagct cgagacccat ctcggcgact tcgtgggagt ggacggcatc
     1081 atcgccgatc tcggcgtgag ctcgccccag atcgacgatc cctcgcgcgg catgagcttt
     1141 cgcagcgaag ggcccatcga catgcgcatg ggggatacgg aaggggagac cgccctcgac
     1201 ctcatcgagc gagtcggtag cgaggagctg gctgacatca tctaccaata tggcgaagag
     1261 cgcagatcgc gccggatcgc gcgctgcatt caccaggcct accgggcggg cgagctccga
     1321 acgacgctcg acttgcgccg cgccgtggtg cgggccgttg gccctgcgcg cgtgggtggc
     1381 gtcgatcccg cgacgcgtac gtttcaagct ctacgcattg cggtcaaccg cgagctcgaa
     1441 gagctgaagg ctctgctcga ggttgcgccg cgggtgctct tgccgtcagc gcccttggtg
     1501 atcatttcct ttcactcgtt ggaagatcgg ctcgtcaagc gcgccttttt ggagtcggcg
     1561 acgtgggagc gcatgacgcc caagcccgtc gttccttcgc gcgtcgaaac ggagcagaac
     1621 ccgcgctcgc gcagcgccaa gcttcgggcg gcgcgaaagg tggcgtcgtg aggagcccgc
     1681 gccccttctt gattctgtgg accctggccg tcgcggccac cgtcgcagcc ttcgttctgc
     1741 acctcgcctt gcgaggccga aacatggagc tggggtacga cctcggcaaa tcgcgcggcg
     1801 agcaggcgcg gctgcgcgag gtgaaacgcg tgctcgagct cgaggcggcg agctacgaga
     1861 ccccgcaacg tgtcgaggtc gtggcgcgca cgcttctcgg gatgcagcca ccggccgccg
     1921 atcggatcat tcccgtcact ggcct
//
LOCUS       DASVII010000677         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_642764,
            whole genome shotgun sequence.
ACCESSION   DASVII010000677 DASVII010000000
VERSION     DASVII010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642764"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VF309_08790"
     CDS             <1..312
                     /locus_tag="VF309_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466724.1"
                     /translation="WPLADRQAPAVHVKASDGVEHGLRRAVDGTRRTWQSVGERGEAW
                     GGGEHRMDLQPTSQQATHHQLALGDEAAPFDAEVAVAHVPVVGEARIVWIGDGLKLRR
                     G"
     gene            complement(354..1013)
                     /gene="lipB"
                     /locus_tag="VF309_08795"
     CDS             complement(354..1013)
                     /gene="lipB"
                     /locus_tag="VF309_08795"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7466725.1"
                     /translation="MADLLSARVLDLGVRDFGEVWAMQRELVAARQKDEVPDTLLFVE
                     HPHVITLGRGSHRENLVAVGDIPTFEIERGGDVTYHGPGQLVGYPIFLLRQNERDLHV
                     YLRNLEEALLRTVADFGIEGGRNPGWTGVWTDGAMGPLGPKRKLASIGVAVKRWVTMH
                     GFALNVSTDLARFAAINPCGLQATVMGSIASVLHRPVEMATVKSCARAHFSDVFGRAF
                     A"
     gene            complement(1054..>1598)
                     /locus_tag="VF309_08800"
     CDS             complement(1054..>1598)
                     /locus_tag="VF309_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7466726.1"
                     /translation="IERGFVPVDRQLRTNVPGVYAIGDLVGQPMLAHKASHEAEVVAE
                     VIAGHRAEMDVRCIPAVIFSDPEIASAGITADEAEKQGRKVKIGKFPFAALGRAIANA
                     DTDGFVKVVIDAASKEVLGIHVVGNGASDIIAEAALSIEMGALADDLSLTIHAHPTLP
                     EAIMEAAKASLGEAIHIMNR"
BASE COUNT          269 a          545 c          538 g          246 t
ORIGIN      
        1 tggccgctcg ccgatcgcca ggcacccgcg gtgcacgtga aagccagcga tggcgtcgag
       61 cacgggctgc gtcgcgcggt agacgggaca cgacgcacgt ggcagagcgt cggcgagcgc
      121 ggcgaggcgt ggggcggtgg cgagcaccga atggacttgc agcccacttc ccagcaagcg
      181 acgcaccacc agctcgctct cggcgacgaa gcggccccct tcgacgctga ggtcgcggtc
      241 gcgcatgtgc cggtagtcgg cgaggcgcgg atcgtctgga tcggtgacgg attgaaactg
      301 cggcggggat gagggcgggg cttgcacctc cgggctatgg acagcgggcg acttcacgcg
      361 aaggcgcgtc cgaagacatc gctgaagtgg gcgcgcgcgc acgacttcac cgtggccatc
      421 tcgacaggac gatgaaggac actggcaatc gagcccatca ccgtcgcttg cagtccgcac
      481 ggattgatcg cggcgaagcg cgccagatcg gtcgagacat tcagcgcgaa cccgtgcatg
      541 gtgacccagc gcttcaccgc caccccgatc gatgcgagct tgcgcttcgg tccgagcggg
      601 cccatcgcgc cgtcggtcca cacgccggtc caacccggat tgcgcccgcc ctcgatgccg
      661 aagtcggcga cggtgcggag cagcgcctcc tccaggttgc gcagatatac gtgcagatcg
      721 cgttcgttct gccgcaataa aaagatcggg tagcccacca gctggcctgg cccgtgatac
      781 gtcacgtcgc cgccacgctc tatctcgaag gtggggatgt cgccgaccgc caccaggttc
      841 tcgcggtggc tgccccgccc gagcgtgatg acgtgggggt gctcgacgaa gagcagggtg
      901 tcggggacct cgtccttctg gcgggccgcg accagctcgc gctgcatcgc ccacacttca
      961 ccgaaatccc ggacgccgag atcgaggacc cgggcggaga gcagatcggc cacgcgcgaa
     1021 atctagccca accgggccgg acgtgcccgc ggatcagcga ttcatgatgt ggatcgcctc
     1081 gccgaggcta gccttggccg cttccatgat cgcctccggc agcgtcgggt gcgcgtggat
     1141 ggtcagcgac agatcgtcgg ccagcgcgcc catctcgatg ctgagggccg cctcggcgat
     1201 gatgtcggag gcgccgttgc cgaccacgtg gattccgagc acctccttgg aggcggcatc
     1261 gatgaccacc ttcacgaagc catcggtatc cgcgttggcg atggcgcgcc ccagcgccgc
     1321 gaaggggaac ttgccaatct tgaccttgcg accttgcttc tcggcctcgt cggcggtgat
     1381 cccggccgac gcgatctcgg gatcgctgaa gatcacggcc gggatgcagc gcacgtccat
     1441 ctccgcgcga tgccccgcga tcacctccgc gaccacctcc gcctcgtgcg aggccttgtg
     1501 cgccaacatg ggctggccca ccagatcgcc gatcgcgtac acgccaggaa cgttggtgcg
     1561 aagctggcga tccaccggca cgaaaccgcg ctcgatct
//
LOCUS       DASVII010000678         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_391560,
            whole genome shotgun sequence.
ACCESSION   DASVII010000678 DASVII010000000
VERSION     DASVII010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391560"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF309_08805"
     CDS             <1..184
                     /locus_tag="VF309_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7466727.1"
                     /translation="FASHVLQSSGDLRAVQEMLGHASISSTQVYTHLDFQHLAKVYDA
                     AHPRARRARPATAKKR"
     gene            190..1209
                     /locus_tag="VF309_08810"
     CDS             190..1209
                     /locus_tag="VF309_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7466728.1"
                     /translation="MAPSVITPVTVLTGFLGSGKTTILNRLVSDPRFADSAVIVNELG
                     DVPIDHLLVRRASESIVVLPGGCICCRASGDLIRALRELHRERASGAIGPFRRVVIET
                     TGLADPAPILAALIELPVLAARYSLAGVVATADAEHGMAALDAHPEAVKQLALADRIV
                     ITKIDRADADALEARLRALNPGAKIVRASHGNLDPALLFETGLHRPGLAAPDATGWLA
                     EGAYRRVGEPRASPHDPRISSFVWRAPQPVAWEDFERGLETLLDLMGAKILRLKGLVS
                     VAGEPGPRAVHAVQHALYPPARLAAWPDADHGTRIVVIGRDLEEAAAARILDSFTRSS
                     AISPR"
     gene            1215..>1944
                     /locus_tag="VF309_08815"
     CDS             1215..>1944
                     /locus_tag="VF309_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7466729.1"
                     /translation="MAATRTPDKDLIPVTILTGFLGAGKTTLLNRILTEQHGEKIAVI
                     ENEFGETGIDNDILVKDKEEQIVEMNNGCICCTVRGDLVRILGDLGKKRREGKLAFDR
                     VIIETTGLANPGPVAQTFFMDEAIHDQYLLDAVVTVVDAKHAQKTLDDHDEARRQVGF
                     ADRILLSKTDLVEDEKVDALVKRLRQMNPRAHQAKVHMGNADIKEILDIRGFNLNSVL
                     EIDPAFLEDDHHEHDDDVKSFVWRD"
BASE COUNT          338 a          683 c          640 g          283 t
ORIGIN      
        1 gttcgcctcc cacgtgctgc aatcctcggg cgaccttcgc gcggtccagg agatgctcgg
       61 ccacgcgagc atctcctcca cgcaggtcta cacgcacctc gacttccagc atctcgcgaa
      121 ggtctacgac gccgcgcatc cacgcgcgcg gcgcgcacgc cccgccacgg cgaaaaagcg
      181 ctgaaggccg tggccccgag cgtcatcacg ccggtcacgg tgctcacggg ctttctcggc
      241 agcggcaaga cgacgatcct caatcgcctc gtctccgacc cgcgcttcgc cgacagcgcc
      301 gtgatcgtca acgagctggg cgacgttccc atcgatcacc tgctggtgcg gcgggcgagc
      361 gagagcatcg tcgtgctgcc gggcggatgc atctgctgcc gcgcttcggg cgatctcatc
      421 cgcgcgctgc gcgagctgca tcgcgagcgc gcgagcggcg cgatcggccc cttccggcgc
      481 gtcgtgatcg agacgacggg gctcgccgac cccgcgccga ttctggcggc gttgatcgag
      541 ctgccggtgc tcgcggcgcg ctattccctg gccggagtcg tcgcgaccgc ggacgcggag
      601 cacggcatgg ccgcgctcga cgcgcatccc gaggccgtca agcagctggc gctcgccgat
      661 cgcatcgtca tcacgaagat cgaccgcgcc gatgccgatg ccctcgaggc gcgcctgcgc
      721 gcgctcaatc cgggggcgaa gattgttcgc gcgagccacg ggaacctgga tccggcgctt
      781 ctcttcgaga cgggcctgca tcgtcccgga ctcgcagcgc ccgatgcgac cggctggctc
      841 gcggagggcg cctaccgccg cgtcggggaa ccgcgagctt cgccccatga cccgcgcatc
      901 tcgagcttcg tatggcgcgc gccgcaaccc gtggcgtggg aagatttcga gcgcgggctg
      961 gagaccctcc tcgatctcat gggggcgaag atcctgcgcc tcaaggggct cgtgagcgtc
     1021 gcgggggagc cggggccgcg cgcggtgcac gcggtgcagc atgcgttgta tcctccggca
     1081 cggcttgccg catggcccga cgccgaccac ggcacgcgca tcgtcgtcat cgggcgggac
     1141 cttgaagaag ccgccgccgc ccgcatcttg gactccttca cccggagttc agcaatctca
     1201 ccgaggtaag ccgcatggcc gcgacacgga cacccgacaa ggacctgatc cccgtgacga
     1261 tactcacggg cttcctcgga gcgggcaaga cgacgctcct caaccgcatc ctcaccgagc
     1321 agcacggcga gaagatcgcg gtgatcgaga acgagttcgg cgagaccggc atcgacaacg
     1381 acatcctggt gaaggacaag gaagagcaga tcgtcgagat gaacaacggg tgcatctgct
     1441 gcaccgtgcg cggcgacctg gtgcgcatcc tgggagatct cggcaagaag cgcagggaag
     1501 gcaagctcgc gttcgaccgc gtcatcatcg agaccacggg cctcgccaat ccgggccccg
     1561 tggcgcagac gttcttcatg gacgaggcga tccacgacca gtacctgctc gacgccgtgg
     1621 tgaccgtggt ggacgcgaag cacgcgcaga agaccctcga cgaccacgac gaggcgcgcc
     1681 gccaggtggg cttcgccgac cggatcctcc tttcgaagac cgatctcgtc gaggatgaaa
     1741 aggtcgacgc gctggtcaag cgcctgcgcc agatgaaccc gcgcgcgcac caggcgaagg
     1801 tccacatggg aaacgccgac atcaaggaga tcctcgacat ccgcgggttc aacctgaatt
     1861 cggtcctcga gatcgacccg gccttcctcg aggacgacca ccacgagcac gacgacgacg
     1921 tgaagtcctt cgtgtggcgg gacg
//
LOCUS       DASVII010000679         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_715140,
            whole genome shotgun sequence.
ACCESSION   DASVII010000679 DASVII010000000
VERSION     DASVII010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715140"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1414)
                     /gene="tcuA"
                     /locus_tag="VF309_08820"
     CDS             complement(20..1414)
                     /gene="tcuA"
                     /locus_tag="VF309_08820"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX7466730.1"
                     /translation="MDPSRTYDLIILGGGNAALCAAITARRSGLAVLVVEAAPIHFRG
                     GNSRHTRNMRTMHAAPLDVLTGAYPEEEYWQDLLKVTGGLTDERLARIAIRETERHLP
                     WMKQHGVRFQAPLGGTLHLSRTNAFFLGGGKALMNAYYAAAGALGVEVWYDSEVVDIA
                     LADGRFTSATVRTKGALHEIRARAVVAASGGFESNLAWLREAWGPPADNFIVRGTPYN
                     MGAVLKLLLAKGARQVSDPTQGHCVAIDSRSPKFDGGIVTRVDAVSLGIVVNKHARRF
                     YDEGEDYWPKRYAIWGRLVAAEPDQIGFSIIDHKSMGKFMPPVFPPVKAGSLAELAVL
                     LGLDPAALEATVAQFNAAVRPGTFDHTILDDCATSGLAIPKTHWARALDTPPYYAYPL
                     RPGITFTYLGVAIDAKARMLMQDGKPAVNVFAAGEIMAGNVLGKGYLAGIGMAIGTTF
                     GRIAGAEAARHVRG"
     gene            complement(1414..>1873)
                     /locus_tag="VF309_08825"
     CDS             complement(1414..>1873)
                     /locus_tag="VF309_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX7466731.1"
                     /translation="VQMRNSRRGRMAILTLDDGTARVEVVVFGELFHEKRSVVQEDQV
                     VVVKGKIFPDEFSGGLRITAESLMDLAEVRAAHARLLRLSINGQADSAKLKALLTQYA
                     GGKCHVAIRYRNAQGECDIRLPENCRVKVSGPLLDSLTEWLDEKNVEVVY"
BASE COUNT          290 a          640 c          647 g          296 t
ORIGIN      
        1 cagcgcctcg agcgtgggcc tagccgcgga catggcgggc cgcctccgcg ccggcgatgc
       61 gcccgaacgt cgtgccgatc gccatgccga tgccggcgag gtagcccttg ccgagcacgt
      121 tgcccgccat gatctcgccc gcggcgaata cgttgaccgc gggcttgccg tcctgcatca
      181 gcatgcgcgc cttggcatcg atcgcgacac ccaggtaggt gaaggtgatg ccggggcgca
      241 gcggatacgc atagtacggt ggcgtgtcga gggcgcgcgc ccaatgcgtc ttcgggatcg
      301 cgaggccgga ggtcgcgcaa tcgtcgagga ttgtgtggtc gaaggtgccg ggccgcaccg
      361 cggcgttgaa ctgcgcgacc gtggcttcca gcgcagcggg gtcgaggccg aggagcacgg
      421 cgagctcggc gagagagccc gccttcaccg gcgggaacac cggcggcatg aacttgccca
      481 tcgacttgtg atcgatgatc gagaagccga tctggtccgg ctcggctgcc accaggcgcc
      541 cccagatcgc gtagcgcttc ggccagtaat cctcgccttc gtcgtaaaaa cgccgtgcgt
      601 gcttgttcac cacgatgccg agcgagacgg cgtccacgcg cgtcacgatg ccgccgtcga
      661 acttgggcga gcgcgagtcg atcgcgacgc agtgcccctg cgtgggatcg ctcacctgcc
      721 tggcgccctt ggcgagcagc agcttcagga ccgcacccat gttgtacggc gtgccgcgca
      781 cgatgaagtt gtcggcgggc gggccccacg cttcgcgcag ccacgcgaga ttggactcga
      841 aaccgcccga ggccgcgacc acggcacgag cacggatctc gtgcagcgcg cccttggtgc
      901 gcaccgtggc ggaggtgaat cgcccgtcgg cgagcgcgat gtccaccacc tcgctgtcgt
      961 accagacctc cacgcccagc gccccggcgg ccgcgtaata ggcgttcatg agcgccttgc
     1021 cgccgcccag gaaaaaggcg ttggttcgcg acaggtgcag cgtgccgccc aagggcgcct
     1081 ggaagcgcac gccgtgctgc ttcatccacg gcaggtgcct ctcggtctcg cggatggcga
     1141 tgcgcgccag cctctcgtcg gtaagcccgc cggtgacctt gaggagatcc tgccagtact
     1201 cctcctcggg atacgcgccg gtgaggacgt cgagcggggc cgcgtgcatg gtgcgcatgt
     1261 tgcgcgtgtg gcggctgttg ccgccgcgga aatggatcgg cgccgcctcg accacgagca
     1321 ccgcgaggcc ggagcgccgc gcggtgatgg cggcgcacag tgccgcgttg ccgcccccga
     1381 ggatgatgag gtcgtaggtc cgggagggat ccactagtag acgacttcga cgttcttctc
     1441 gtcgagccac tcggtgagcg agtcgaggag cggacccgac accttcaccc ggcaattctc
     1501 cggcaggcgg atgtcgcact cgccctgcgc attgcggtag cggatcgcca cgtggcactt
     1561 gccgccggca tattgggtga ggagcgcctt cagcttcgcc gagtcggcct ggccgttgat
     1621 cgacaggcgc aggagccgcg cgtgcgcggc gcgcacctcg gccagatcca tgaggctttc
     1681 cgccgtgatg cgcagccccc ccgagaactc gtcgggaaag atcttgccct tcacgaccac
     1741 gacctggtct tcctggacca cggagcgctt ttcgtggaag agctcgccga acacgaccac
     1801 ctccacgcgc gcggtgccat cgtcgagcgt gaggatcgcc atgcgcccgc gccgcgaatt
     1861 gcgcatctgc acg
//
LOCUS       DASVII010000680         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_607146,
            whole genome shotgun sequence.
ACCESSION   DASVII010000680 DASVII010000000
VERSION     DASVII010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607146"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /gene="rsmD"
                     /locus_tag="VF309_08830"
     CDS             complement(<1..578)
                     /gene="rsmD"
                     /locus_tag="VF309_08830"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7466732.1"
                     /translation="MRRSPGASSAGGQRGAPAGRNRVRIIAGKWRSRLVKFPPAAHLR
                     PTPDRVRETLFNWLGQRLDGLACLDLFAGSGALGFEAMSRGASRVVLVESDREVAAAL
                     RETARVLEAEGVEIVVSDAIAYLRRTPEKFDIAFIDPPFASDLAVRAMAELRDRLQAG
                     ARVYVESGAALPPPEWGRLVREDRAGAVRYAL"
     gene            654..1607
                     /gene="ftsY"
                     /locus_tag="VF309_08835"
     CDS             654..1607
                     /gene="ftsY"
                     /locus_tag="VF309_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7466733.1"
                     /translation="MPPLFSKKTPSEAAAGSGLKSFLRKDLELTRERWRGALGVLRAR
                     PKIDESLYEELESQLLSADVGVAATQGLIESLRKAQRDKRLEDASQLEGELKSALVAL
                     LRPLEKALPADPPRPFVILLAGVNGAGKTTTIAKLTRLFQGEGKSVLLAAGDTFRAAA
                     REQLMEWGRRHDVTVNSQDKGDSAAVIFDAIQSARAKAIDVVLADTAGRLTTQLHLMD
                     EIRKVKRVMSKADASAPHETWLVLDANTGQNAVRQVKAFDEALQLTGLIVTKLDGTAK
                     GGAVAAIAREHPVPIRFIGIGEKAEDLRVFIAADFVEALFA"
     gene            1604..>2203
                     /locus_tag="VF309_08840"
     CDS             1604..>2203
                     /locus_tag="VF309_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7466734.1"
                     /translation="MIQFSSVAKRYPGGHEALREVTVEIAAGEMVAVTGHSGAGKSTL
                     LKLAAGIERASSGTVVVHGQNLGALGETALAVLRRRIGFVFQDHKLLFDRNAFENVAL
                     PLRVAGSAGDEIARRVRAALDKVGLLAREKAMPVALSGGEQQRLCIARAIVNRPAILV
                     ADEPTGNLDADYARAILELFGSFHQVGVTVLLSSHDESSL"
BASE COUNT          356 a          771 c          762 g          314 t
ORIGIN      
        1 taaagggcat agcgtaccgc cccggcacga tcttcgcgca ccaggcgccc ccattcgggc
       61 ggcgggagcg cggcaccgct ctcgacgtac acgcgcgcgc cggcttggag ccggtcccgc
      121 agctccgcca tcgcgcgaac cgcgaggtcg ctcgcgaaag gcggatcgat gaacgcgatg
      181 tcgaatttct ccggggtcct gcgcagatag gcaatggcat ccgaaacgac gatctcgacg
      241 ccctcggcct cgaggacacg cgccgtttcg cgaagcgccg cggcgacctc gcgatcgctc
      301 tcgaccagga ctacacggga cgcgccccgc gacatcgcct cgaatccaag agctccgctg
      361 cccgcgaaaa ggtcgaggca tgcgaggccg tcgaggcgtt ggcccagcca gttgaagagc
      421 gtctcgcgga cgcgatcggg ggtgggacgc aggtgcgcgg cggggggaaa tttgacgagg
      481 cggctgcgcc acttgccggc gatgatgcga acgcggttgc gcccggccgg agcgccgcgc
      541 tgtcctcctg cgctcgaagc gcccggggag cgtcgcatgg cggtcatcgt gcggcggatc
      601 ataccgtaaa atattcgctc tcccgcccca cggccacgcc ttcggccgca atcatgcctc
      661 ccctgttttc gaagaagact cccagcgaag ccgccgccgg ctccggcctc aaatcgttcc
      721 tgcgcaagga tctcgagctc actcgcgaga gatggcgggg cgcgctcggg gtgctgcgcg
      781 cgaggccgaa gatcgacgag agtctctacg aggagctcga gtcccagctt ctctcggccg
      841 acgtcggcgt cgcggcgacc cagggtctca tcgagagtct gcgcaaggcg cagcgcgaca
      901 agcgcctcga ggacgcctcg cagctcgagg gggagctgaa gagcgcgctg gtcgccctcc
      961 tgcgcccgct cgagaaggcg ctccccgcgg atcccccgcg acccttcgtg atcctcctcg
     1021 ccggcgtgaa tggcgcgggc aagaccacca ccatcgccaa gctcacgcgt ctctttcagg
     1081 gcgagggaaa gtcggtgctc ctcgccgcgg gcgacacgtt ccgggccgcg gcgcgggagc
     1141 agctgatgga gtggggacgg cgccatgacg tgacggtaaa ctcgcaggac aagggtgact
     1201 cggcggcggt gatcttcgat gcgatccagt cggcgcgcgc caaggcgatc gacgtggtcc
     1261 tcgccgacac cgcgggacgc ctcacgacgc agctccacct catggacgaa atccgcaagg
     1321 tgaagcgcgt gatgtcgaag gccgacgcgt cggcgcccca tgagacgtgg ctcgtcctcg
     1381 acgcgaacac cggccagaac gccgtgcgac aggtgaaggc gttcgacgag gcgctgcagc
     1441 tgacgggcct catcgtgacc aagctcgacg gcaccgccaa gggcggcgcg gtcgcggcga
     1501 tcgcacgcga gcatccggtg ccgatccgct tcatcggcat cggcgagaaa gcagaggacc
     1561 tgcgcgtgtt catcgccgcg gacttcgtcg aggccctctt cgcgtgatcc agttttcgtc
     1621 ggtggcgaag cgctacccgg gcggccacga ggcgctgcgg gaagtgacgg tcgagatcgc
     1681 cgccggcgag atggtcgccg tcaccggcca ctcgggcgcc ggcaagtcga cgctcctcaa
     1741 gctcgccgcg ggcatcgagc gcgcctcgag cggcacggtc gtggtccacg gccagaacct
     1801 gggcgcgctc ggcgagaccg cgctcgcggt gctgcgccgg cgcatcggct tcgtcttcca
     1861 ggaccacaag ctcctcttcg acaggaacgc gttcgagaac gtcgcgctgc cgctgcgcgt
     1921 agcgggctcc gcgggagacg agatcgccag gcgcgtgcgt gccgcgctcg acaaggtggg
     1981 gctcctcgcg cgcgagaagg cgatgccggt ggccctttcc ggcggcgagc agcagcgcct
     2041 gtgcatcgcg cgcgccatcg tcaaccggcc cgcgatcctg gtcgccgacg agcccaccgg
     2101 caacctcgac gccgactacg cacgcgcgat cctggagctc ttcggctcct tccaccaggt
     2161 gggcgtgacg gtgctccttt ccagccacga cgagtcgagc ctc
//
LOCUS       DASVII010000681         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_139933,
            whole genome shotgun sequence.
ACCESSION   DASVII010000681 DASVII010000000
VERSION     DASVII010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139933"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF309_08845"
     CDS             complement(<1..186)
                     /locus_tag="VF309_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX7466735.1"
                     /translation="MNISPTQELVDDIRRGRMVVLADAEDRENEGDLVLAADFVTPEA
                     INFLATFARGLVCLTLTE"
     gene            complement(240..836)
                     /locus_tag="VF309_08850"
     CDS             complement(240..836)
                     /locus_tag="VF309_08850"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX7466736.1"
                     /translation="MFTGIVTSVGRIESARPGAEGVRLRVEAQALGMDDVRVGDSIAV
                     QGVCLTVVARDAQGFEADVSRATLEVTHGLEGGRAVNLEKSLRASDRIGGHIVAGHVD
                     GVGTVTAFDDLGGSFRLAVDVAPQLSRYIARKASITVDGVSLTVNEAGDSSFVVNIIP
                     HTRAITTFRGLAAGSRVNLEVDLLARYVERILQASPDR"
     gene            complement(839..1933)
                     /gene="ribD"
                     /locus_tag="VF309_08855"
     CDS             complement(839..1933)
                     /gene="ribD"
                     /locus_tag="VF309_08855"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX7466737.1"
                     /translation="MPFTLEDHAFMVRALALTERGRDTCTPNPNVGCVIVKDGRVIAE
                     GWHERAGEAHAEARALAAAKESVEGATVYVTLEPCSHHGRTPPCAEALVSAKVGRVVA
                     ALEDPNPKGKGQGAARLREAGIRVDVGLMAAEALEAHRGFVWRMTRGRPWMRVKAASS
                     LDGRVALANGQSKWITGEAARRDVHMLRARSCAMLTGIGTVLADDPELTVRHVPCSRQ
                     PKRVVIDSRLDMPLAAKILSGDPPIILTVSEDAGRRKALEARGAEVVTVAREGEKTDL
                     GAIARLLAERGFNEVTVETGGKLMGSLLKARVVDELVIDYAPLILGDKSQALFVMPEW
                     TALGEAPRAKIVDVRRIEEDIRVTARFEGA"
     gene            complement(1935..2387)
                     /gene="nrdR"
                     /locus_tag="VF309_08860"
     CDS             complement(1935..2387)
                     /gene="nrdR"
                     /locus_tag="VF309_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX7466738.1"
                     /translation="MKCPFCSAEDTQVIDSRVSEEGDSIRRRRRCQVCNKRFTTYETA
                     ELRMPQIVKTDGVREDFSSAKLRTGFQRALHKRPVTTELVDAAITRIEQKMLALGERE
                     IDSRRLGEMVMEELRKLDKVAYIRFASVYKSFSDVEDFRDAIREVKDK"
     gene            complement(2387..>2573)
                     /locus_tag="VF309_08865"
     CDS             complement(2387..>2573)
                     /locus_tag="VF309_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466739.1"
                     /translation="PAMTTRGFKDGEAAQVAHYIADVLESHAHAAEIAKVGEAVNAMC
                     ARFPVYGPAMRATYGMK"
BASE COUNT          373 a          907 c          865 g          428 t
ORIGIN      
        1 ctcggtgagc gtgaggcaga cgaggcctcg cgcgaaggtc gcgaggaagt tgatcgcctc
       61 gggcgtcacg aagtcggcgg cgagcacgag atcgccctcg ttctcgcggt cctccgcatc
      121 ggcgagcacg accatgcgcc cgcggcgaat gtcgtcgacg agttcctggg tcgggctgat
      181 attcatggaa tcatctcggc ggactcttgc aagaccgcta ttttgccatt ttcgctcgac
      241 tatcgatccg gggacgcctg caggatgcgc tccacgtagc gcgcgaggag atcgacttcg
      301 aggttcacgc ggcttccggc ggcgaggccg cggaaggtcg tgatggcgcg ggtgtgcgga
      361 atgatgttca ccacgaagga cgaatcgccc gcctcgttca cggtgaggct cacgccgtcc
      421 acggtgatcg aagccttgcg cgcgatgtag cgcgagagtt ggggggccac gtcgaccgcc
      481 aggcgaaagc ttccgccgag gtcgtcgaac gcggtcacgg tgcccacgcc gtccacgtgc
      541 cccgcgacga tgtggccgcc gatacgatcc gaagctcgca gggatttttc caggttcacc
      601 gcgcggccgc cctcgaggcc gtgggtcacc tcgagcgtgg cgcgcgacac gtcggcctcg
      661 aagccctggg cgtcgcgggc gacgaccgtg aggcacacgc cctgcaccgc gatcgagtcg
      721 ccgacgcgta cgtcgtccat gccgagggcc tgggcctcga cgcgcaggcg cacgccctcg
      781 gcgccggggc gcgcgctctc gatcctgccc accgacgtca cgatccccgt gaacatgttc
      841 aggccccctc gaagcgcgcg gtgacgcgaa tgtcttcctc gatcctgcgc acgtcgacga
      901 tcttcgcgcg cggcgcttcg ccgagggcgg tccactcggg catcacgaag agcgcctgcg
      961 acttgtcgcc gaggatgagg ggcgcgtaat cgatcaccag ctcatccacg acccgcgcct
     1021 tcaagagcga gcccatcagc ttgccgccgg tctcgaccgt gacctcgttg aagccgcgct
     1081 cggcgaggag ccttgcgatg gcgccgagat cggtcttctc gccttcgcgc gccacggtca
     1141 ccacctcggc gccgcgcgct tccagggcct tgcgccgtcc cgcgtcctcg ctcaccgtca
     1201 ggatgatcgg cggatcgccc gagagaatct tcgcggcgag aggcatgtcg aggcggctat
     1261 cgatcaccac gcgcttcggc tggcgcgagc acggcacgtg acgcaccgtg agctcgggat
     1321 cgtcggcgag caccgtgccg atgccggtca gcatcgcgca cgatcgcgcg cgcagcatgt
     1381 gcacgtcgcg gcgcgcggcc tctccggtga tccacttgct ttgcccgttg gcgagcgcga
     1441 cgcgcccgtc gagcgagctc gccgccttga cgcgcatcca gggacgcccg cgcgtcatgc
     1501 gccacacgaa gccgcgatgc gcctcgagcg cctccgcggc catgagcccc acgtcgacgc
     1561 ggattcccgc ctcgcgcagg cgggcggcgc cctggccttt ccccttcgga ttcgggtcct
     1621 cgagcgccgc gaccacgcgg cccaccttcg cggagacgag cgcctcggcg cagggaggcg
     1681 tgcggccgtg gtggctgcac ggctcgagcg tgacgtagac cgtcgcgcct tcgacggact
     1741 ccttcgccgc cgcaagcgcc ctcgcctccg catgcgcctc gccggcgcgc tcgtgccacc
     1801 cctcggcgat cacgcggccg tccttgacga tcacgcagcc cacgttcggg ttgggcgtgc
     1861 acgtgtcacg gccccgctcg gtgagcgcca gcgcgcgcac catgaaagcg tgatcttcga
     1921 gggtgaacgg caaattactt gtctttgact tcccggatgg cgtcgcggaa atcctcgacg
     1981 tcggagaagc tcttgtagac cgaggcgaag cggatgtacg caaccttgtc gagcttgcga
     2041 agctcctcca tcaccatctc ccccaggcgc cgcgagtcga tctcgcgctc gccgagggcg
     2101 agcatcttct gctcgatgcg cgtgatggcc gcgtcgacga gctccgtggt gaccgggcgc
     2161 ttgtgcaacg cccgctggaa gccggtgcgc agcttcgcgg aggaaaagtc ctcgcgcacg
     2221 ccgtcggtct tcacgatctg gggcatgcgc agctccgcgg tctcgtaggt cgtgaagcgc
     2281 ttgttgcaga cctggcagcg gcggcgccgg cggatggagt cgccttcctc gctcacgcgc
     2341 gagtcgatga cctgggtgtc ctcggcgctg cagaaggggc atttcattat ttcatcccgt
     2401 acgtcgcgcg catggcggga ccatacaccg ggaagcgcgc gcacatcgcg ttcaccgcct
     2461 cgcccacttt cgcgatctcg gccgcgtgcg cgtgggactc gagcacgtcg gcgatgtagt
     2521 gcgccacctg ggccgcctcg ccgtccttga acccgcgagt ggtcatggcg ggc
//
LOCUS       DASVII010000682         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_139936,
            whole genome shotgun sequence.
ACCESSION   DASVII010000682 DASVII010000000
VERSION     DASVII010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139936"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF309_08870"
     CDS             complement(<1..323)
                     /locus_tag="VF309_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466740.1"
                     /translation="MTFLAPRIEARFPPSKIRDRGFPRLSERNAAARCIGPIHESDES
                     DTDVSAGDHRRSGSPPTVAGRRPDYDERKAGLTASIVKVRKGFPMNATSLRVTLLATA
                     TAAALL"
     gene            362..673
                     /locus_tag="VF309_08875"
     CDS             362..673
                     /locus_tag="VF309_08875"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEX7466741.1"
                     /translation="MRSNTSLNSSPIARDIQNVVSDAQELLKTVQTEGESKMGDVKAK
                     VQLQLDSARQMLGQLQATVQDGAKVAVDGADEYVRSNPWRAVGISAALGALIGFIAAR
                     R"
     gene            685..1080
                     /locus_tag="VF309_08880"
     CDS             685..1080
                     /locus_tag="VF309_08880"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7466742.1"
                     /translation="MIRESTGIVGDVKGLASTGLRAVRTRLELLAIEITEEKAWAVRF
                     LIVALAALYLLSFGLLLAIFALVVWASAENRPGILAAFAGVFLLLGAGGLAYILAASK
                     QRHPIFTETIAVLKGDEHALGAGLRAAGD"
     gene            1073..1297
                     /locus_tag="VF309_08885"
     CDS             1073..1297
                     /locus_tag="VF309_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466743.1"
                     /translation="MTELEILRRRRELVVLSAGLQRATLARRLQRIDQHPAHAVLRFA
                     AGAVTLPIVWKVAWSIAAAKLFPSTKSLKR"
     gene            1317..1484
                     /locus_tag="VF309_08890"
     CDS             1317..1484
                     /locus_tag="VF309_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX7466744.1"
                     /translation="MLYWAAVFFIIALIAAVLGFGGIAAGAAGIAKILFFIFLVAFVI
                     SLLMGVFRRSA"
BASE COUNT          256 a          534 c          535 g          277 t
ORIGIN      
        1 agcagggcgg ccgcggtggc ggtcgcgaga agggttacgc gcaaggaagt cgcattcatg
       61 gggaatcctt tcctgacttt gacgatggag gcggtgaggc ccgctttgcg ttcatcgtag
      121 tccgggcgcc gtccggccac cgtcggcggc gagcccgacc gtctgtggtc cccggccgac
      181 acgtccgtgt cggactcgtc cgactcgtga atcggaccaa tgcaacgcgc cgccgcgttc
      241 cgctccgaca ggcgcgggaa gcccctgtcc ctaatcttgc tgggtgggaa gcgtgcctcg
      301 atgcgaggcg cgaggaacgt catcggccga agccgaatcg ccaaagatca aggagatcga
      361 catgcgcagc aacaccagcc tgaacagcag cccgattgca cgcgacatcc agaacgtcgt
      421 ctcggatgcg caggagctct tgaaaaccgt ccagaccgaa ggggaaagca agatgggaga
      481 tgtgaaggcg aaggtccagc tgcagctcga ctcggcgcgg cagatgctcg gccagctcca
      541 ggcgacggtg caggacggcg cgaaggtcgc ggtcgacggt gccgatgaat acgttcgcag
      601 caacccgtgg cgcgcggtgg gcatcagcgc cgcgctgggc gcgctgatcg gcttcatcgc
      661 ggcacgccgc taggagcccc gcgcgtgatt cgcgaatcca cgggcatcgt cggcgacgtc
      721 aagggactcg cctcgacggg gctgcgtgcg gtgcgaacgc gcctggagct gctcgcgatc
      781 gagatcacgg aggaaaaggc gtgggcggtg cgattcctca tcgtcgccct ggccgcgctc
      841 tacctcctga gcttcgggct gctgctcgcg atcttcgccc tcgtggtgtg ggcatccgcg
      901 gagaaccggc cgggcatcct ggccgcgttc gcgggtgtat tcctcctcct gggcgcgggc
      961 gggctcgcct acatcctcgc cgcttccaag cagcgccatc cgatcttcac cgagacgatc
     1021 gcggtgctca agggcgacga gcatgccctg ggggcgggac tgcgagcggc cggtgactga
     1081 gctcgaaatc cttcgccgcc gccgcgagct cgtcgtgctc tcggcggggc tgcaacgcgc
     1141 gaccctcgcg cgccgcctgc agcgaatcga ccagcatccg gcgcacgccg tgctgcggtt
     1201 tgccgccggc gccgtgaccc tgccgatcgt gtggaaggtc gcctggtcga tagccgccgc
     1261 gaaactcttc ccctccacga agtccctgaa acgttaaccc tcgaaaggag acacacatgc
     1321 tttactgggc agccgtattc ttcatcatcg ccttgatcgc cgcggtcctg ggcttcggcg
     1381 gaatcgccgc cggcgccgcc ggcatcgcca agatcctttt cttcatcttc ctcgtcgcat
     1441 tcgtcatttc tctgctgatg ggcgtgttcc gccgctcggc gtgacgcagc aacctcccat
     1501 cgcgtggacg ccggccgcgt gatccgcggc cggccgcggg tcaatgcctg atcgtctcgt
     1561 cgatctctat cagcgccttg ccgacagggt ggaggagatc ga
//
LOCUS       DASVII010000683         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_679097,
            whole genome shotgun sequence.
ACCESSION   DASVII010000683 DASVII010000000
VERSION     DASVII010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679097"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VF309_08895"
     CDS             <1..235
                     /locus_tag="VF309_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7466745.1"
                     /translation="NVVTNDSGLMHVAAALDRPTAAIFGSSSPAFTPPLSSRARVITL
                     KLECSPCFQRECPLGHTNCLVTLEPALVLEAIG"
     gene            232..1266
                     /locus_tag="VF309_08900"
     CDS             232..1266
                     /locus_tag="VF309_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7466746.1"
                     /translation="MSYAIDPDITVARTLPGGFYSDAAAFERSRERVFARTWQWLGEL
                     ADVASPASLSPRDLLPGFMDEALLLARDESGALRCLSNVCTHRGNILVKSACAAKDIR
                     CGYHSRRFDLAGRMAFMPEFREAKDFPSPSDDLPRVALAQWAGHGFASLDPVAPFADF
                     AAGMESAVAAMPLDRMRHDASRDRDYGIRAHWALYVENFLEGFHIPFVHSGLNAIVDY
                     PAYRTELHRWSNVQVANAKDGAPAAQYWWIFPNLMLNFYPWGLSLNLVLPQAIDRTRV
                     LFRSYVWDASTLDRGAGAALDRVEAEDEAIVEAVQRGVRSRLYSRGRYSPAREAGVHH
                     FHRLLCELLE"
     gene            1337..2224
                     /locus_tag="VF309_08905"
     CDS             1337..2224
                     /locus_tag="VF309_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX7466747.1"
                     /translation="MKLRNLAVLGLLAAAPAFAADPAYVKSVEDWRAHAEQSLRKDNG
                     WLTLAGRYAMKPGLNTFGTGEKNDIVFPKGLGPDRMGTVTVEPGRVFVKLEPGLAMVK
                     DGIAMTDKVMGTDLENRDWVAMGRAAFHVIERDGKYILRLADNESEVRSHFGGRVWYG
                     VDKGYLVPAKFVAYDPARKIPIVNILDEVSDETAPGYVEFKLKGKAYKLDALEEDDGL
                     FIILRDGTAGRTTYGSGRFLFVEKKPKPGEAFMLDLNRVYNPPCAFSEYTTCPLPPKQ
                     NILKVKIEAGEKYPPRKQG"
     gene            2226..>2492
                     /locus_tag="VF309_08910"
     CDS             2226..>2492
                     /locus_tag="VF309_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466748.1"
                     /translation="MAFSRAALCLAIAATAASAADTAQWPPPTPVQERMHELQLLIGS
                     REATPAQREAAREELSSLLKSPAGQARGPTPDEKPLRPARAPLDP"
BASE COUNT          409 a          896 c          790 g          397 t
ORIGIN      
        1 gaacgtggtg accaacgatt cgggattgat gcacgtcgcc gccgccctcg atcgcccgac
       61 cgccgcgatc ttcggctcgt cgagcccggc tttcacgccg ccgctttcgt cgcgggcgcg
      121 cgtcatcacg ctgaagctcg agtgcagccc ctgcttccag cgcgagtgcc cgctcggcca
      181 taccaactgc ctcgtcaccc tcgagcccgc gctcgtcctc gaggcgatcg gttgagctac
      241 gcgatcgatc ccgacatcac cgtcgcgcgc acgctgccgg gcggcttcta ttccgacgcg
      301 gcggccttcg agcgctcgcg cgagcgcgtc ttcgcgcgca cctggcaatg gctcggcgag
      361 ctcgccgacg tcgcctcgcc ggcgagcctg tcgcctcgcg acctgctccc cggattcatg
      421 gacgaggcgc tccttctcgc ccgcgacgaa tccggcgccc tgcgttgcct ctccaacgtg
      481 tgcacccatc gcggcaacat cctcgtgaag tcggcgtgcg cggcgaagga cattcgctgc
      541 ggctaccatt cgcggcgttt cgacctcgcg gggcgcatgg ccttcatgcc ggagttccgc
      601 gaggcgaagg atttcccgtc gccttccgac gacctgcccc gggtcgcgct cgcgcaatgg
      661 gccggccacg gattcgcctc cctcgatccg gtcgcgcctt tcgcggactt cgccgccggc
      721 atggaaagcg ccgtcgccgc gatgccgctc gatcgcatgc gccatgacgc ctcgcgcgac
      781 cgcgactacg ggatccgcgc gcactgggcg ctctacgtgg aaaatttcct cgagggattc
      841 cacatcccgt tcgtccactc gggattgaac gcgatcgtcg actacccggc ctatcggacc
      901 gagctgcatc gctggtcgaa cgtgcaggtc gccaacgcga aggacggggc acccgccgcg
      961 cagtactggt ggatctttcc gaacctcatg ctcaatttct atccgtgggg actgtcgctg
     1021 aacctcgtcc ttccccaggc gatcgatcgc actcgcgtcc tcttccgatc ttacgtctgg
     1081 gatgcctcga cgctcgaccg cggagccggc gccgccctcg accgcgtgga ggccgaggac
     1141 gaggcgatcg tcgaagcggt gcagcgcggc gtgcgctcga ggctttactc ccgcgggcgg
     1201 tattcgcccg cccgcgaggc gggagtccac catttccacc gcctgctgtg cgagctgctc
     1261 gagtgacggg ccggcgccgc gccgtgaaac cactacaatc tcccgcagtc caacgtgcat
     1321 cggggaattc gtgaccatga aactgcgcaa tctcgccgtg ctcggccttc tcgccgccgc
     1381 gcccgcgttc gccgccgatc ccgcctacgt gaagtcggtc gaggattggc gcgcccacgc
     1441 cgagcagagc ctgcgaaaag acaacggctg gctcacgctc gccggccgct atgcaatgaa
     1501 gcccggcctc aacacgttcg gaaccggcga aaagaacgac atcgtgttcc cgaagggcct
     1561 cggccccgat cgcatgggca ccgtgacggt cgagcccggg cgcgtcttcg tgaagctcga
     1621 gcccggcctc gcgatggtga aggacggcat cgcgatgacc gacaaggtga tgggcaccga
     1681 tctcgagaac cgcgactggg ttgcgatggg acgggcggcg ttccacgtga tcgagcgcga
     1741 cggaaagtac atcctgcgcc tcgccgacaa cgagagcgaa gtccgcagcc atttcggcgg
     1801 gcgcgtctgg tacggcgtgg acaagggcta tctcgttccc gcgaaattcg tcgcctacga
     1861 cccggcgcgc aagatcccga tcgtgaacat cctcgacgag gtctccgacg agaccgcgcc
     1921 cggatacgta gagttcaagc tgaagggaaa ggcgtacaag ctcgatgcgc tcgaagagga
     1981 cgacggcctc ttcatcatcc tgcgagacgg caccgcggga cgcacgacct acggatcggg
     2041 ccgcttcctg ttcgtcgaga agaagccgaa accgggcgaa gcgttcatgc tcgacctcaa
     2101 tcgcgtctac aaccccccgt gcgcgttcag cgagtacacc acctgcccgc tgccgccgaa
     2161 gcagaacatc ctcaaggtga agatcgaggc gggcgagaaa tacccgcctc gcaagcaggg
     2221 ctgaaatggc gttctcccgc gccgcgctct gcctcgcgat cgccgcaacg gcggcctcgg
     2281 cggcggatac ggcgcagtgg cctccgccca cgccggtgca ggagcgaatg cacgagctgc
     2341 agctcctgat cggcagccgc gaggcgacgc ccgcgcagcg tgaagccgcg cgcgaggagc
     2401 tctcgagcct cctcaagagc ccggcgggac aggcgcgcgg gccgacgccc gacgagaaac
     2461 cgttgcgacc cgcgcgcgcg ccgctcgatc cg
//
LOCUS       DASVII010000684         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_175530,
            whole genome shotgun sequence.
ACCESSION   DASVII010000684 DASVII010000000
VERSION     DASVII010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175530"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1054
                     /locus_tag="VF309_08915"
     CDS             29..1054
                     /locus_tag="VF309_08915"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX7466749.1"
                     /translation="MLPLLPTTIAGSLPKPAWLAEPRKLWAPWKLEGEALAEGKRDAV
                     RLVLEMQERSGIDIVTDGEQTRRHFVNGYMETLTGIDFKNLKTVRIRNRYDANVPRVV
                     GPVSRPAPVHSEDAKFLRSATENAIKFTLPGPMTMVDTLFDEHYGSREKLAFAFAEIL
                     NQEARELAAIGIDVIQFDEPAFNVYFDEVRDWGVAALERAREGVTAKPAVHICYGYGI
                     EANIVWKKTLGNEWRQYEQIFPLLAKSTIDQVSLECANSRVPIDLIALLEGKDVLVGC
                     IDVATNRVETPEDVAKVIREALKHVPRERLYPCTNCGMVPLPREVAAGKLEALGAGAA
                     LVRAELV"
     gene            1087..1224
                     /locus_tag="VF309_08920"
     CDS             1087..1224
                     /locus_tag="VF309_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEX7466750.1"
                     /translation="MMIGIAVAVALVVGYLLVMRVFFKDSKELDKKIDYAKMKEWKDE
                     D"
     gene            complement(1221..2453)
                     /locus_tag="VF309_08925"
     CDS             complement(1221..2453)
                     /locus_tag="VF309_08925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7466751.1"
                     /translation="MRRVAVTGIGIISPLGNDARAFAAALEAGRPGIAPLTGKFHERL
                     ASPVGASVAFDGANRFPAPQLRMLDRVSQFALAAAAQALGESGAVFQDLDLDRAGVFV
                     GTGMGGARTTDESYYTLYGEPSDRLKPFSVLLAMNNAAASWIGMEHGLRGANLTYSTA
                     CSSSAVAIGEAARRVASGEADVMIAGGSEAPLNFGTLKAWDAMRTLAAEDPQDASASC
                     KPFARDRSGLVLGEGAAIVVLEDWDRAVGRGARIRAEVAGYGLCADPSHITRPSVAGQ
                     AAAMRRALADARMSVAEIDYINAHGTGTQANDSVETAAIKEVFGARAHAIPVSSTKSM
                     HGHLLGAAGALEFAASLLAMEAGVVPPTLNLRHPDPECDLDYVALVARGGLDIGAFMS
                     NSFAFGGTNAVLVARRAP"
     gene            complement(2456..>2707)
                     /locus_tag="VF309_08930"
     CDS             complement(2456..>2707)
                     /locus_tag="VF309_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7466752.1"
                     /translation="RLHSLLAKDFELKPEAARPETRLEDLDIDSLRMIEILFSVEDAF
                     GITVPAEQAELREKVKTVGDLAAYIDTLVAEQHGAGKGA"
BASE COUNT          441 a          943 c          903 g          420 t
ORIGIN      
        1 ttttaattcg tacctgacac catttctcat gctccccctg cttccaacca cgatcgccgg
       61 tagtcttccg aagcccgcgt ggctcgccga gccgcgcaag ctctgggcgc catggaagct
      121 cgagggcgag gcgctcgccg agggcaagcg cgacgcggtg cgcctggtgc tcgagatgca
      181 ggagcgctcg ggcatcgaca tcgtcaccga cggcgagcag acgcgccggc atttcgtgaa
      241 cggctacatg gagacgctca ccggcatcga cttcaagaac ctgaagacgg tgcgcatccg
      301 caatcgctac gacgcgaacg ttccgcgcgt cgtgggcccg gtctcgcggc ccgcgccggt
      361 ccactccgaa gacgcgaagt tcctgcgctc ggccaccgag aacgcgatca agttcacgct
      421 tccgggcccg atgacgatgg tcgacacgct cttcgacgag cattacggct cgcgggagaa
      481 gctcgcgttc gccttcgccg agatcctgaa ccaggaagcg cgcgagctcg ccgcgatcgg
      541 catcgacgtg atccagttcg acgagcccgc gttcaacgtc tacttcgacg aggtgcgcga
      601 ttggggcgtg gccgcactcg agcgagcgcg cgagggtgtc acggcaaagc cggcggtgca
      661 catctgctac ggctacggca tcgaggccaa catcgtctgg aagaagactc tcggcaacga
      721 gtggcgccag tacgagcaga tcttcccgct gctcgccaag tccaccatcg accaggtgtc
      781 gctcgagtgc gccaactcgc gcgtgcccat cgatctcatc gcgctcctgg agggcaagga
      841 cgtgctggtc ggctgcatcg acgtcgcgac caatcgcgtc gagacgcccg aagacgtggc
      901 gaaggtcatc cgcgaagcgc tgaagcacgt gccccgcgag cgcctctatc cctgcaccaa
      961 ctgcggcatg gtcccccttc cccgcgaggt cgccgccggc aagctcgaag ccctcggcgc
     1021 gggcgccgca cttgttcgcg cggagctggt ctaaccgtct ttccgggagc cccaatggac
     1081 caggccgtga tgatcggtat cgccgtggcg gtcgcgctcg tcgtcggcta cctgctggtg
     1141 atgcgcgtct tcttcaagga cagcaaggag ctcgacaaga agatcgacta cgccaagatg
     1201 aaggagtgga aggacgagga ctagggagcc cggcgggcga cgagcaccgc gttggttccg
     1261 ccgaacgcga atgagttgga catgaacgcg ccgatgtcga ggccgcccct cgccactagc
     1321 gccacgtagt cgaggtcgca ttccggatcg ggatggcgca ggttcagcgt gggcggcacg
     1381 acgcccgcct ccatcgcgag caacgacgcc gcgaattcga gcgcgcccgc ggcgcccagc
     1441 aaatggccgt gcatcgactt ggtcgagctc accggaatcg cgtgggcgcg cgcgccgaac
     1501 acttccttga tcgccgcggt ctcgacgctg tcgttcgcct gcgttcccgt cccatgcgcg
     1561 ttgatgtagt cgatctccgc gacgctcatg cgcgcatcgg cgagagcgcg gcgcatggcc
     1621 gccgcctggc ccgcgacgct gggccgggtg atgtgcgacg gatcggcgca aagcccgtag
     1681 cccgccacct cggcgcggat gcgcgcaccg cgccccaccg cccgatccca atcctcgagc
     1741 acgacgatcg ccgcgccttc gcccagcacg aggccgctgc gatcgcgggc gaacggcttg
     1801 cacgaggccg aggcgtcttg cggatcctcg gcggcgagcg tgcgcatcgc atcccatgcc
     1861 ttcagcgtgc cgaagttgag cggcgcctcg ctcccgcccg cgatcatcac gtccgcttcc
     1921 ccgctcgcga cgcgccgcgc cgcttcgccg atcgcgaccg ccgaggagga gcacgcggtc
     1981 gaataggtga gattggcgcc gcgcagcccg tgctccatgc cgatccacga cgcggcggcg
     2041 ttgttcatcg cgaggagcac gctgaagggc ttcaggcgat ccgatggctc gccgtagagc
     2101 gtgtagtagc tctcgtcggt ggtgcgggcg ccgcccatgc cggtgccgac gaagacgccc
     2161 gcgcgatcga gatcgaggtc ctggaaaacg gcgcccgact cgcccagcgc ctgggccgct
     2221 gccgcgagcg cgaactggct cacgcgatcg agcatgcgca gctgcggggc gggaaaacga
     2281 ttcgcgccgt cgaatgcgac cgacgcgccg acggggctcg ccaggcgctc gtggaatttt
     2341 ccggtgagcg gcgcgatccc cggtcgcccg gcctcgaggg ccgcggcgaa agcgcgcgcg
     2401 tcgttgccga gcggcgagat gatgccgatg ccggtaacgg cgacgcgcct caccttcagg
     2461 cgcccttgcc tgccccgtgc tgctcggcga ccagcgtatc gatgtaggcc gcgaggtcgc
     2521 cgaccgtctt caccttctcg cgcagctccg cctgctccgc gggcaccgtg atcccgaacg
     2581 catcctccac gctgaagagg atctcgatca tgcgcagcga atcgatgtcg aggtcttcca
     2641 ggcgcgtctc gggccgcgcc gcctcgggct tcagctcgaa gtccttggcg aggagggagt
     2701 gcaggcg
//
LOCUS       DASVII010000685         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_103446,
            whole genome shotgun sequence.
ACCESSION   DASVII010000685 DASVII010000000
VERSION     DASVII010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103446"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..979)
                     /locus_tag="VF309_08935"
     CDS             complement(161..979)
                     /locus_tag="VF309_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466753.1"
                     /translation="MSEETLVDVESVSFAYGRRPILKGISMRVPRGKVVAIMGASGSG
                     KTTLLRIIGGQLKPNAGAVRIAGQAVHELDGEALYRMRRKMSMLFQFGALFTDMSVFE
                     NVAFPLREHTRLPEEMIRDLVLMKLEAVGMRGAHRLRPGELSGGMARRVALARAVVLD
                     PMLVMYDEPFAGLDPIALAVIGQLIRRLNDALGSTSIIVTHDVLESLNIVDYLYFISA
                     GVIVGEGTPEEIRRSELPYVRQFVHGETEGPVPFHYPAKPYAADVGLGGEARRA"
     gene            1052..>1610
                     /locus_tag="VF309_08940"
     CDS             1052..>1610
                     /locus_tag="VF309_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7466754.1"
                     /translation="MKKPRLLLVDDDALISESLAFALAGDYEVTRAGDRVAAVSALRE
                     GLRPDLALIDLGLPPVAHLPNEGFKLIGDLLAHAPQVRILVLTGQNEESNARRARALG
                     AIELVPKPCEPQLLRSLLARALQAHALDKRDDDAEQVKALVGESPPLRRLRSQIDLYA
                     ASAFPALIQGESGSGKERVAFSLHHL"
BASE COUNT          252 a          563 c          556 g          239 t
ORIGIN      
        1 gagcacgccg ctgaagtaga cctcgcgcat caccaggcgg aagcgctgga agctcgtgcc
       61 cgacgaggcg agcaccagga cgaagaagcg cgccatcgtt cccgcgcgcc agatgccgtt
      121 gatcacgttc gcgccgaggc gcgcgagcgg cttcctcacg tcaagcacgg cgcgcctccc
      181 cgcccagtcc cacgtccgcg gcgtagggct tcgccgggta gtggaaggga accgggcctt
      241 cggtttcgcc gtgcacgaac tggcgcacgt acggcagctc cgagcggcgg atttcctcgg
      301 gagtgccctc gcccacgatc acgccggcgg agatgaagta gaggtagtcg acgatgttca
      361 gcgactcgag cacgtcgtgc gtgacgatga tcgaggtgga tccgagggcg tcgttcagcc
      421 tgcggatgag ctgcccgatc acggcgaggg cgatcgggtc gaggccggcg aagggctcgt
      481 cgtacatgac gagcatcggg tcgagcacca ccgcgcgcgc gagggccacg cgtcgcgcca
      541 tgccgccgga cagctcgccg gggcgcaagc ggtgcgcccc gcgcatgccc acggcctcca
      601 gcttcatcag cacgaggtcg cggatcatct cttccggaag gcgcgtgtgc tcgcgcagcg
      661 ggaacgccac gttctcgaag acgctcatgt cggtgaagag cgcgccgaac tggaacagca
      721 tgctcatctt gcggcgcatc cggtagagcg cctcgccgtc gagctcgtga accgcttggc
      781 cggcgatgcg caccgcgccc gcgttgggct tcagctgtcc gccgatgatg cgcagcagcg
      841 tcgtcttgcc gctgccggag gcgcccatga tcgccacgac cttgccgcgc ggcacgcgca
      901 tggagattcc cttgaggata gggcgccggc cataggcgaa gctcaccgac tcgacgtcga
      961 ccagggtttc ttcggacacg cggggatatt gcgaggacaa gggatcgtat tttaacagct
     1021 ttgggggggc gccctcccct ataatccccg cgtgaagaag ccccgcctgc tgctggtcga
     1081 cgacgacgcg ctcatctccg aaagcctcgc gttcgcgctg gcgggggact acgaggtcac
     1141 gcgcgccggc gaccgcgtcg cggcggtgtc ggccctgcgt gaggggctgc gccccgacct
     1201 cgccctcatc gacctgggcc tgccgcccgt cgcgcacctg cccaacgagg gcttcaagct
     1261 gatcggcgac ctcctcgcgc atgcgccgca ggtgcgcatc ctggtcctca ccggccagaa
     1321 cgaggagtcg aacgcgcgcc gcgcgcgcgc cctgggcgcc atcgagctgg tgcccaagcc
     1381 gtgcgagccg cagctgctgc gctcgctcct cgcgcgcgcg ctgcaggcgc atgcgctcga
     1441 caagcgcgac gacgacgcgg agcaggtgaa ggcgctggtc ggcgagagcc cgccgcttcg
     1501 ccgcctgcgc agccagatcg acctctacgc ggcgtccgcg tttccggcgt tgatccaggg
     1561 cgagtccggc agcggcaagg agcgcgtcgc attctccctg catcacctga
//
LOCUS       DASVII010000686         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_248462,
            whole genome shotgun sequence.
ACCESSION   DASVII010000686 DASVII010000000
VERSION     DASVII010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248462"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VF309_08945"
     CDS             <1..641
                     /locus_tag="VF309_08945"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7466755.1"
                     /translation="DRDYHAFDAVARVLVESPVVAVRADSRWKSLQDLVADARAHPKE
                     ISVGHSGIGSHTHLSLVALFRAAHAEVNEIPFGATQVVPSLLGGHVDALVQLPAALSG
                     QVKQGTLRLLATLTPNRDPALPDVPTAREQGVDVSLEAWRGIAAPHGTPRPVIETLEA
                     AIRRTVESPEFAKGSESLGVRPAFMPAHKFDELIAWEDADVAWLMRSVGLKK"
     gene            851..>2537
                     /locus_tag="VF309_08950"
     CDS             851..>2537
                     /locus_tag="VF309_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX7466756.1"
                     /translation="MNDLSAGRAADSHSLGDDAGEDVIRIRGARQNNLKNLDVDLPTG
                     ELIVVTGPSGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDAIEGIPPA
                     IAIDQTNPVRTSRSTVGTMTEINDHLKLLFARTASLFCRNCSQPVRRDTSETIFAQMK
                     ERAAAAGDPRLVIAFPIDVPKNFSEEEITKLLQAQGYTRIHHREGSRLEVAQDRVKMS
                     SAEKSRVVEAIEAALRVGTGRVNVFVGEEGAEAWRFSTDLHCAHCDIPYSDPTPARFS
                     FNSPIGACDACRGFGRVIGVDYGLVIPDETKTLGGGAVKPFQTKSFSECQDDLVKYAK
                     KRRIPLDVPYRSLAPEQKHWVLHGDTDWIDWKKTGKTHWYGVKVFFDWLETKAYKMHI
                     RVLLSRYRAYTPCATCDGARLKPDTMLWRVGTRADADGVLAPASRYMPKGTQWSREEL
                     AALPGLCLHDVMLLPADKVEAFFARVKLPAPLDEATELLLGEIRSRLRYLGQVGLGYL
                     TLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMGRVIDVMK"
BASE COUNT          433 a          858 c          848 g          398 t
ORIGIN      
        1 tcgaccgcga ctatcacgcg ttcgacgcgg tggcgcgcgt cctggtggag tcgccggtcg
       61 tggcggtccg cgccgattcc cgctggaaga gccttcagga cctggtcgcc gatgcgcgcg
      121 cgcatccgaa ggagatcagc gtgggccact cgggaatcgg cagccacacg cacctctcgc
      181 tcgtggcgct gttccgcgcg gcgcacgccg aggtgaacga gattcccttc ggcgcgacgc
      241 aggtggtgcc aagcctgctc ggcgggcatg tggatgcgct ggtgcagctg cccgcggcgc
      301 tctcgggaca ggtcaagcag ggaacgctgc gcctgctcgc gacgctcacg cccaaccgcg
      361 atcccgcgct gcccgacgtt ccgaccgcgc gcgagcaagg cgtcgacgtc tcgctcgagg
      421 cgtggcgagg catcgccgca ccccacggca cgccgcgccc ggtgatcgag acgctcgaag
      481 cggcgattcg ccgcaccgtg gaatccccgg aattcgcgaa gggcagcgag agcctcggcg
      541 tgcggcccgc cttcatgccc gcgcataagt tcgacgagct gatcgcctgg gaggatgccg
      601 acgtcgcatg gctgatgcga tccgtcggat tgaagaagta acccagcgag agagggttga
      661 aaccgtcgac gttcgccctc aggtcatgtg cataacccgg tagaattgtg ggtgcgttgg
      721 tcgagttatc cacagcgcgc aagacggaaa ataattcctg ccggaagcgt ccgggagatg
      781 cctagaatgg gcttctctcc cgccaaatat tcagcgcccg cacgacgcgg tcgctctcac
      841 caggccctcc atgaacgacc tgtccgcggg acgcgccgcc gattcccatt ccctgggcga
      901 tgacgccggc gaagacgtga tccgcattcg cggagcgcgc cagaacaacc tcaagaacct
      961 cgacgtcgac ctgcccacgg gggagctcat cgtcgtgacg ggtccttccg gttcgggcaa
     1021 gtcgtcgctc gtgttcgaca cgctctatgc cgaggggcag cgtcgctacg tggagacgtt
     1081 ctccccgtac gcgcggcagt tcctcgaccg catggacaag ccgcaggtcg acgcgatcga
     1141 gggcattccc ccggcgatcg ccatcgacca gacgaacccg gtgcgcacct cgcgctccac
     1201 cgtcggcacg atgacggaga tcaacgatca cctgaagctg ctcttcgccc gcaccgcgag
     1261 cctcttctgc cgcaactgct cgcagcccgt gcggcgcgac acgtcggaga cgatcttcgc
     1321 gcagatgaag gaacgcgccg cggccgccgg cgatccgcgc ctcgtcatcg cgttccccat
     1381 cgacgtgccg aagaatttca gcgaggagga gatcacgaag ctcctccagg cgcagggcta
     1441 cacgcgcatc caccatcgcg aaggctcgcg cctcgaggtg gcgcaggacc gcgtgaagat
     1501 gtcgagcgcg gagaagtcgc gcgtggtcga ggcgatcgag gcggcgctgc gggtggggac
     1561 ggggcgcgtg aatgtgttcg tcggggaaga aggcgccgaa gcgtggagat tctccaccga
     1621 cctccattgc gcgcactgcg acattcccta ctccgatccg acgccggcgc ggttctcgtt
     1681 caactcgccg atcggcgcgt gcgatgcgtg ccgcggattc ggacgcgtga tcggcgtgga
     1741 ctacggcctg gtgattcccg acgagacgaa gacgctcggc ggcggcgcgg tgaagccctt
     1801 ccagacgaag agcttctccg agtgccagga cgacctggtg aagtacgcga agaagcgccg
     1861 catcccgctc gacgttccct accgcagcct cgcgcccgag cagaagcact gggtcctcca
     1921 cggcgacacc gactggatcg actggaagaa gaccggcaag acgcattggt acggcgtgaa
     1981 ggtcttcttc gactggctcg agaccaaggc gtacaagatg cacatccgcg tgttgctgtc
     2041 gcgctaccgc gcgtacacgc cgtgcgcgac ctgcgacggc gcgcggttga agcccgacac
     2101 gatgctgtgg cgcgtcggca ccagggccga tgcggacgga gtgctcgcac ccgccagccg
     2161 ctacatgccg aagggcacgc agtggtcgcg cgaggagctc gcggcgctgc cgggcctgtg
     2221 cctgcacgat gtgatgctgc tgcccgccga caaggtcgag gcgttcttcg cgcgcgtgaa
     2281 gctccccgcg ccgctggatg aagcgaccga gctcctgctc ggcgagatcc ggtcgcggct
     2341 gcgctacctg ggtcaggtcg gcctgggcta cctcacgctc gatcgccagt cgcgcacgct
     2401 ctcgggcggc gaggtgcagc gcatcaacct cacgaccgcg ctcggcacct cgctcgtcaa
     2461 cacgttgttc gtgctcgacg agccctcgat cggattgcat ccgcgcgaca tggggcgcgt
     2521 catcgacgtg atgaagc
//
LOCUS       DASVII010000687         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_140363,
            whole genome shotgun sequence.
ACCESSION   DASVII010000687 DASVII010000000
VERSION     DASVII010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140363"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VF309_08955"
     CDS             complement(<1..1341)
                     /locus_tag="VF309_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466757.1"
                     /translation="MTRALSIERLRHDGEEFLQALSREFYEAHSGLKPSAELGPIYQK
                     YAPILGEEALEVTRELFLGAAEGSEEQRSARILLDWEVESQSSRQLTELDEREIAWEG
                     DALVRLEDGRQVPYQKVSIELGNSTDARERAMIERARAALVQRELAPLKRERFQRERD
                     ITESLDLGGDYNATWERLSGISLSGLRAECEAFLRDTQAMWDDVAPRFVKRTLGMEIA
                     EMSRADALALFRAREFDAYFPANDMDPSIRKQVREMGVDPDAGGRVLYDIGEREGKRS
                     RAFCAPVRVPEEVYLVLRPHGGQTDWNTYLHELGHALHFAYMRPDLPMEFRHMGDNSV
                     TEGYAMLFDHLMQDAGWLQRYTGLEKSSAPTFLRSAGFEELHFLRRYSAKLIYETQLY
                     GGEESFDALPDLYVELLTKATSFQYSPADAFVDVDPRFYSARYLRAWQHQALLTE"
     gene            complement(1338..>1536)
                     /locus_tag="VF309_08960"
     CDS             complement(1338..>1536)
                     /locus_tag="VF309_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7466758.1"
                     /translation="VDYDETGSIGKRYRRQDEVGTPFCITVDGDSVKDATVTVRDRDT
                     LKQERIGIDAVRGYLQDRLPG"
BASE COUNT          259 a          546 c          470 g          261 t
ORIGIN      
        1 ctcggtgagc agtgcctggt gctgccacgc gcggagatag cgcgccgagt agaaacgcgg
       61 gtcaacgtcc acgaaggcgt cggcaggtga atactgaaag ctcgtcgcct tcgtgagcag
      121 ctcgacatag agatccggca acgcatcgaa cgactcctcg ccgccataga gctgcgtctc
      181 gtagatcagc ttcgccgaat agcgacgcag gaagtgcagc tcctcgaagc cggcgctgcg
      241 cagaaaggtg ggcgccgatg acttctccag tcccgtgtac cgctgcagcc atccggcatc
      301 ctgcatcaga tggtcgaaca gcatggcgta cccttccgtc accgagttgt cgcccatgtg
      361 gcgaaactcc atggggagat cgggacgcat gtacgcgaag tggagcgcat gccccagctc
      421 gtgcaggtag gtgttccaat ccgtctgccc gccgtgcggg cgcagcacga gatacacctc
      481 ctcgggcacg cgcaccggcg cgcaaaaggc gcgcgagcgc ttcccttcgc gctcgccgat
      541 gtcgtacagc acccgtccac ccgcatcggg gtcgacgccc atctcgcgca cctgcttgcg
      601 aatggacgga tccatgtcgt tcgccggaaa gtacgcgtcg aactcccgcg cgcggaacag
      661 cgcgagcgcg tcggcgcgcg acatctcggc gatctccatg ccgagcgtgc gcttcacgaa
      721 tcgcggcgcc acgtcgtccc acatcgcctg cgtatcgcgc aagaacgcct cgcactccgc
      781 gcgcaggccg ctcagcgaga tcccgctcaa ccgctcccag gtggcgttgt agtcgccccc
      841 gagatcgagc gactcggtga tgtcacgctc gcgctggaaa cgctcgcgct tcaggggggc
      901 cagctcgcgc tgcaccagcg cggcacgcgc gcgctcgatc atcgcacgct cgcgcgcgtc
      961 ggtgctgttg cccagctcga tggacacctt ctgataggga acctggcggc catcctccag
     1021 ccggacgagc gcgtccccct cccacgcaat ctcccgctca tcgagctcgg tgagctgccg
     1081 gctgctctgc gattccacct cccaatccag gagaatgcgc gccgagcgct gctcctcgct
     1141 cccctcggcc gcgccgagaa agagctcccg agtcacctcg agcgcttcct cgcccaggat
     1201 cggagcatac ttctgataaa tggggccgag ctccgccgat ggcttcaggc cgctgtgcgc
     1261 ctcgtagaac tcccgcgaca gcgcttggag aaactcctcg ccatcgtggc gcaggcgctc
     1321 gatggacagc gcccgtgtca tccgggcagc cggtcttgga gatagccgcg caccgcgtcg
     1381 atgccgatgc gctcctgctt cagcgtatcg cggtcccgaa cggtgacggt ggcatctttc
     1441 accgaatcgc cgtccacggt gatgcagaag ggtgtgccca cttcgtcctg ccggcggtac
     1501 cgcttgccga tgctgcccgt ttcgtcgtag tccacc
//
LOCUS       DASVII010000688         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_32911, whole
            genome shotgun sequence.
ACCESSION   DASVII010000688 DASVII010000000
VERSION     DASVII010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32911"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VF309_08965"
     CDS             complement(<1..779)
                     /locus_tag="VF309_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7466759.1"
                     /translation="MKPNDMKPAFKWDDPLLLEDQLTEDERMVRDSARSYAQGELMPR
                     ILQANRHEKFDRAILTEMGALGFLGSTIEGYGCAGVNYVSYGLVAREIERVDSAYRSA
                     MSVQSSLVMHPIHAYGNEEQRQRWLPGLAKGELVGCFGLTEPGHGSDPNGMVTRARKA
                     DGGFKVSGAKMWITNAPIADVFVVWAKDDAGEIRGYVLEKGMKGLSAPKIEGKFSLRA
                     SVTGEVVMDEVFVPDANLLPNVKGLKGPFGCLTRARYGISWG"
     gene            850..>2164
                     /locus_tag="VF309_08970"
     CDS             850..>2164
                     /locus_tag="VF309_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466760.1"
                     /translation="MARNDRLTVKHLWKLERPAQPTLSPDGAQACVSVATHDMDENTS
                     RSSLWLYSAFGGEPRRLTSCGEKDGEPKWSPDGRWIAFVAKRPAVGNEKADDEPQLYL
                     IAPDGGEARRVTDIATGVFGIKWFPGSRRVAFLSWVWPEEKGLKALAKRYKAFKDDKV
                     KAHVVEHSAYRWWDHWLSDGRVPHLFTVDIESGAVRDLFAGTRYELARADPTAHLYDI
                     SPDGREIAFSFDPAEDKRFDHSYQLVALDVSRKRFRTLSARSPLNHETPRYSPDGRWI
                     GLVTQDLRKSPVDTQRLALIDRRSGKLTVASSRWDRSVVPPLVWSADSSAVHFVAEDN
                     ARQHLYRLEIGKRSPEIAARGGVVSDFDVAGDAIAYVRNDMSTPPGVFWAAPGIAPRR
                     IDRLNQGTLDGVKLGEVREFEIEGWNREKVHMWVVYPPDFDRRKKW"
BASE COUNT          348 a          741 c          719 g          356 t
ORIGIN      
        1 ccccaggaga tgccgtagcg cgcgcgggtg aggcacccga acgggccctt cagccccttc
       61 acgttcggca gcaggttcgc atcgggcacg aacacttcgt ccatcacgac ctcgccggtg
      121 acggaagcgc gcaggctgaa cttgccttcg atcttcggcg cgctgaggcc cttcattccc
      181 ttctcgagca cgtagccgcg gatctcgccc gcgtcgtcct tcgcccagac cacgaacacg
      241 tcggcgatgg gcgcgttggt gatccacatc ttcgcgccgg agaccttgaa tccgccgtcg
      301 gccttgcgcg cgcgcgtgac catgccgttc ggatcggaac cgtggccggg ctcggtgagc
      361 ccgaagcagc cgacgagctc gcccttcgcg agccccggaa gccagcgctg gcgctgctct
      421 tcgttgccgt atgcgtggat cgggtgcatc accagcgacg actgcacgct catcgccgag
      481 cgataggccg aatcgacgcg ctcgatctcg cgcgcgacga gcccgtagct cacgtagttc
      541 acgccagcgc agccatagcc ctcgatcgtc gagccgagga agcctagcgc gcccatctcc
      601 gtgaggatcg cgcggtcgaa cttctcgtgg cgattggcct ggagaattcg cggcatgagc
      661 tcgccctgcg cataggagcg cgcgctgtcg cgcaccatgc gctcgtcctc ggtgagctgg
      721 tcctcgagga gcagggggtc gtcccacttg aacgcgggtt tcatgtcgtt gggtttcatg
      781 gggtctttcg agcgggcgcg atcattctac tgctagaatc cccgcccatt cgctgatcgc
      841 aggcaataca tggccaggaa cgaccgcctc accgtcaagc atctctggaa actcgagcgt
      901 cccgcgcagc cgaccctctc acccgatggc gcgcaggcgt gcgtgtcggt ggcgacccac
      961 gacatggacg agaacacgag ccgctcgagc ttgtggctct actcggcgtt cggcggcgag
     1021 ccgcggcgcc tcacgtcgtg cggcgagaag gacggcgagc cgaagtggtc gcccgacggg
     1081 cgctggatcg cgttcgtcgc gaagcgcccc gcggtgggaa acgaaaaggc cgacgacgag
     1141 ccgcagctct atctcatcgc gcccgatggc ggagaggcgc ggcgcgtgac cgacatcgcg
     1201 acgggcgttt tcggcatcaa gtggttcccc ggctcgcggc gcgtcgcgtt cctctcgtgg
     1261 gtatggcccg aggagaaggg cctgaaggcg ctcgcgaaac gctacaaggc gttcaaggac
     1321 gacaaggtga aggcccatgt cgtcgagcac tcggcctacc gctggtggga ccactggctt
     1381 tccgacgggc gcgtgcccca tctcttcacg gttgacatcg aatcgggcgc ggtgcgcgat
     1441 ctcttcgccg gcacgcgcta cgagctcgcg cgcgccgatc ccacggcgca cctctatgac
     1501 atctcgccgg atgggcgcga gatcgcgttt tccttcgacc ccgccgagga caagcgtttc
     1561 gaccacagct accagctggt cgccctcgac gtgagccgca agcgcttccg cacgctgagc
     1621 gcgcgctcgc ccctcaatca cgagacgccg cgctactcgc ccgacgggcg atggatcggg
     1681 ctcgtcaccc aggaccttcg caagagcccc gtcgacacgc agcgcctcgc gctcatcgac
     1741 cggcgcagcg gcaagctcac ggtggcgtcg tcgcgctggg accgcagcgt cgttccgccg
     1801 ctcgtgtgga gcgccgactc cagcgccgtc cacttcgtcg ccgaggacaa cgcgcgccag
     1861 catctctatc gcctggagat cggcaagcgc tcgccggaga tcgccgcgcg gggcggcgtg
     1921 gtgagcgatt tcgacgtcgc gggcgacgcg atcgcctacg tgcgcaacga catgagcacg
     1981 ccgcccggag tcttctgggc cgctcccggc atcgcgccgc ggcgcatcga tcggctgaat
     2041 cagggcacgc tcgacggcgt gaagctgggc gaggtgcgcg agttcgagat cgaaggctgg
     2101 aatcgcgaga aggtgcatat gtgggtcgtg tatccgccgg atttcgaccg gaggaagaag
     2161 tggc
//
LOCUS       DASVII010000689         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_715796,
            whole genome shotgun sequence.
ACCESSION   DASVII010000689 DASVII010000000
VERSION     DASVII010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715796"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF309_08975"
     CDS             complement(<1..186)
                     /locus_tag="VF309_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466761.1"
                     /translation="MTEEEDRMEYAEQNRLLRRQFDSILTEPIPERMYLKRPAWLDYA
                     RAASFIAIGLAIGLALPM"
     gene            complement(183..677)
                     /locus_tag="VF309_08980"
     CDS             complement(183..677)
                     /locus_tag="VF309_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7466762.1"
                     /translation="MDYNLIVPFIPNLRRYARALVGDRDGADDLVQDTLERAVRKFHL
                     WRPGDLRAWLFSIMHNVFVNQLKARKIPYAVDIDDMAIAAHVPTVTSVDVLDLQNALL
                     GLAPEQREVVLLVALEDMTYSEVSTSLGIPIGTVMSRLSRGREKLRRAMSGEATRSGL
                     RVVQ"
     gene            complement(744..1364)
                     /locus_tag="VF309_08985"
     CDS             complement(744..1364)
                     /locus_tag="VF309_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7466763.1"
                     /translation="MSAKIGVRYEFSGTESLLENSYLTPIFDHLVVAARTLEEGGAWV
                     EEKLGVAPVAGGKHPTMGTHNRLLRIGPREFLEVIAIDPEAPTPARPRWFDLDSPEMR
                     ARLEEGPALIHWVERTADLEAALRDYSEPVQILSFARGPYRWRMGVPRDGRRPGRGSL
                     PTLIQWEGALHPADALPESGCRIEGFTQGNGRITADFAIASGMRTI"
     gene            complement(1379..>1599)
                     /locus_tag="VF309_08990"
     CDS             complement(1379..>1599)
                     /locus_tag="VF309_08990"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX7466764.1"
                     /translation="YLKEIDGLLKGRKWAIGNHFTVVDGYLLVFYRWGNRNGIAVKQM
                     ENYSALAQRVMARPAVKKVMADEGITLD"
BASE COUNT          275 a          539 c          512 g          273 t
ORIGIN      
        1 catcggcagc gcgaggccaa tcgccaaccc gatcgcgatg aaggaagcgg cgcgcgcgta
       61 gtcgagccac gccgggcgct tcaggtacat gcgttccggg atcggctcgg tgaggatcga
      121 gtcgaactgg cgccgcagca gccggttctg ctccgcgtat tccatgcgat cctcttcctc
      181 ggtcattgga cgaccctcag gccgctgcgc gtcgcctcgc cgctcatcgc gcggcgcagc
      241 ttctcgcgcc cccgcgacag gcgcgacatg acggtgccga tggggatgcc gagcgacgtg
      301 ctcacttccg aataggtcat gtcctcgagc gcgacgagca ggaccacctc gcgctgctcc
      361 ggcgcgaggc cgaggagcgc gttctgcagg tcgagcacgt ccacgctcgt caccgtgggg
      421 acgtgcgccg cgatcgccat gtcgtcgatg tcgacggcgt acgggatctt gcgcgccttc
      481 agctggttca cgaacacgtt gtgcatgatg gagaaaagcc aggcgcgaag atcgcccggg
      541 cgccataagt ggaacttccg caccgcccgc tcgagggtgt cctgcaccag gtcgtcggcc
      601 ccgtcgcggt cgcccaccag ggcgcgcgcg taacggcgca agttcggaat gaacgggacg
      661 atcaggttgt agtccatgac ctcgattagt gacggctaat actgcatcaa caatcccggc
      721 ggcgcgctat tccgggggga acctcaaatc gtacgcattc ccgaggcgat agcaaagtcc
      781 gccgtgatac ggccgttccc ttgcgtgaaa ccttcgatcc ggcagccgga ctccgggagc
      841 gcatcggcgg ggtgcagcgc gccttcccac tggatgagcg tgggcaggct cccgcggccc
      901 gggcgccggc cgtcgcgagg cacgcccatg cgccagcgat agggcccgcg cgcgaacgaa
      961 aggatctgga ccggctcgga ataatcgcgc agggccgcct cgagatccgc ggtgcgctcg
     1021 acccagtgga tcaacgcggg gccttcctcg aggcgcgcgc gcatttccgg cgaatcgagg
     1081 tcgaaccagc gcgggcgcgc cggcgtcggc gcttcgggat cgatcgcgat cacctcgagg
     1141 aattcgcgcg gcccgatgcg aaggaggcga ttgtgagtgc ccattgtcgg atgcttgccc
     1201 cccgccaccg gcgccacgcc caacttctcc tcaacccacg cgccgccctc ctccagcgtt
     1261 cgcgccgcaa cgaccagatg gtcgaaaatt ggtgtcaggt acgaattttc gaggagggac
     1321 tccgtccccg aaaattcgta cctgacacca attttcgctg acaccagttt tcgctccctc
     1381 agtcgagagt aatgccttcg tcggccatca ccttcttgac cgccgggcgc gccatcacgc
     1441 gctgcgcgag ggccgagtag ttttccatct gcttcaccgc aatgccgttg cggttgcccc
     1501 agcggtagaa gaccagcagg tagccgtcga ccacggtgaa gtggttaccg atcgcccact
     1561 tgcgaccctt gaggaggccg tcgatctcct tgagatagt
//
LOCUS       DASVII010000690         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_103605,
            whole genome shotgun sequence.
ACCESSION   DASVII010000690 DASVII010000000
VERSION     DASVII010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103605"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VF309_08995"
     CDS             <1..896
                     /locus_tag="VF309_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX7466765.1"
                     /translation="PILVGRPAVLERRIARYGLRLKPGVHFQVVNPEDDPRFRDYWTE
                     YHKLTERRGVSQAYAQIEMRRRHTLIGSMLIHRGDADGLICGTFGTHDLHLHYIDQVI
                     GKRPGVGSYYAMNLLMLPRRTVVICDTYVNVDPSPEQILEMTMLATEEIRRFGLVPKV
                     ALLSHSNFGTSDAPSVIKMQKALALIREKLPHLDVEGEMHGDAALSEEVRLKAFPNSR
                     LKGEANLLIMPTLDAANISFNLLKVVGGEGITVGPILLGAARAVHILTPTATVRRIIN
                     LTALAVVDAQEQEKHAAGRAA"
     gene            893..1276
                     /locus_tag="VF309_09000"
     CDS             893..1276
                     /locus_tag="VF309_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7466766.1"
                     /translation="MNDRFDHLFIRPADFEASLAFYRDVLGWSVSKTWGGNGNGRGAV
                     LSGGGVKVVLAERPSEAGPGAENGARPHVHLDIHDIDARFRALPKGAHIVAEPESTHW
                     GTRWFVVRDPDGNVIAFEEFHGSGG"
     gene            1269..>2065
                     /locus_tag="VF309_09005"
     CDS             1269..>2065
                     /locus_tag="VF309_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX7466767.1"
                     /translation="MAETLRSNLAPLRGVSRRLPRVLPEPATIGICALSGRVDEASMA
                     SGVEYLRDLGHRVVVPDETLHAWRYFAGTDDERLAGFHALLDDASIDLVMAARGGYGL
                     SRILHRIDWNRVATCGKAFVGFSDFTAFNMAAYSCANLVTFHGPMLSIDFGNGHPDSF
                     AQQHFWMALSRDSHHIGEFPCDHGYDARRVEGTLWGGNLSLIAHLAGTPYFPAIDDGI
                     LYVEEIAEEPYAIERLFLQLHHAGVLGRQRAILLGDFTDCRPSNPLR"
BASE COUNT          330 a          723 c          674 g          338 t
ORIGIN      
        1 ggcccatcct ggtggggcgc cccgcggttc tcgagcggcg catcgcgcgc tacgggctgc
       61 gattgaagcc cggcgtgcat ttccaggtgg tcaatcccga ggacgacccg cgcttccgcg
      121 attactggac cgagtaccac aagctcaccg agcgccgcgg cgtctcgcag gcgtacgcgc
      181 agatcgagat gcgccggcgc catacgctca tcgggtcgat gctgatccat cgcggcgatg
      241 ccgacgggct catctgcgga accttcggaa cccacgacct gcacctgcat tacatcgacc
      301 aggtgatcgg caagcggccc ggcgtcggca gctattacgc gatgaacctg ctgatgctgc
      361 cgaggcgaac cgtcgtcatc tgcgacacct atgtgaacgt cgatccgtca cccgagcaga
      421 tcctcgagat gacgatgctc gcgaccgagg agatccggcg cttcgggctg gtgccgaaag
      481 tggcgctgct gtcgcactcg aacttcggca cgagcgacgc gccctcggtg atcaagatgc
      541 agaaggcgct cgcgctcatc cgcgagaaac tgccgcatct cgacgtcgag ggcgagatgc
      601 atggcgacgc ggcgctctcg gaggaggtgc gcctcaaggc ctttccgaac tcgcggttga
      661 agggcgaggc gaaccttctc atcatgccga ccctcgatgc cgccaatatc tccttcaacc
      721 tcctcaaggt ggtcggaggg gagggcatca ccgtcgggcc gatcctcctc ggtgccgcga
      781 gagccgtgca catcctcacg ccgaccgcga ccgtgcgccg catcatcaac ctgacggcgc
      841 tcgcggtggt ggacgcgcag gagcaggaaa agcacgcggc cgggcgcgcc gcatgaacga
      901 tcgcttcgac cacctgttca tcaggcccgc ggatttcgag gcctcgctcg cgttctatcg
      961 cgacgtgctc ggatggtcgg tgagcaagac ctggggcggc aacggcaacg ggcgcggcgc
     1021 cgtgctctcg ggcgggggcg tgaaggtggt gctggcggag cgcccctcgg aagcgggccc
     1081 cggcgcggag aatggcgcgc gtccccatgt ccacctcgac atccacgaca tcgatgcgcg
     1141 tttccgcgcc ttgccgaagg gcgcccacat cgtggccgag cccgagtcga cccattgggg
     1201 cacgcgctgg ttcgtcgtgc gcgatcccga cggcaatgtc atcgcgttcg aagagttcca
     1261 cggcagcggt ggctgagacg cttcgctcga acctggcgcc gctgcgcggg gtttcgcgcc
     1321 ggctgccgcg cgtgcttccc gagcccgcca ccatcggcat ctgcgcgctc tcgggccgcg
     1381 tcgacgaggc gtcgatggcg agcggcgtcg agtacctgcg cgacctgggc caccgcgtcg
     1441 tcgtgcccga cgagaccctg cacgcgtggc gttacttcgc cggcacggac gacgagcgcc
     1501 tcgcgggatt ccacgcgctc ctcgacgacg cgtcgatcga cctggtcatg gcggcgcgcg
     1561 gcggctacgg cctgtcgcgg atcctgcacc gcatcgactg gaaccgcgtg gccacgtgcg
     1621 gcaaggcctt cgtggggttc agcgacttca ccgccttcaa catggcggcc tattcctgcg
     1681 cgaacctcgt gaccttccac ggcccgatgc tctccatcga tttcggcaac ggccaccccg
     1741 acagtttcgc gcagcagcat ttctggatgg cattgagccg cgacagccac cacatcggcg
     1801 aattcccctg cgaccacggc tacgacgcgc gccgcgtgga gggaacgctg tggggaggca
     1861 atctctccct catcgcgcat ctcgcgggaa cgccctactt tcccgccatc gacgacggca
     1921 tcctctacgt ggaagagatc gccgaggagc cctacgcgat cgagcgcctc ttcctgcagc
     1981 tccatcacgc gggcgtcctc gggcgccagc gcgcgatcct gctgggcgac ttcaccgact
     2041 gcaggccgtc gaatccgctg cgcta
//
LOCUS       DASVII010000691         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_535712,
            whole genome shotgun sequence.
ACCESSION   DASVII010000691 DASVII010000000
VERSION     DASVII010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535712"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VF309_09010"
     CDS             <1..747
                     /locus_tag="VF309_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7466768.1"
                     /translation="YFVGGKVHSSWALKTREGIILFDTIYPYNSEELIVGGMHKLGLD
                     PKDIKYLLVSHAHQDHIGGAEMIQKRYGPHVVMSGPDWNLVEKLPNRFKSMAPRRDMV
                     ATDGMKITLGESTVTLWLTPGHTDGTTSYTFQVVDRGRPVNVVYSGGTALVYEWDKPE
                     WSVKNLQTYIDSQRHLAAKAKETGATVLLSNHSEFDEAWKKNRMLAGRGDGPHPYEIG
                     ADWVQRYFKVTESCARATQLMVDKRAGRSG"
     gene            756..1721
                     /locus_tag="VF309_09015"
     CDS             756..1721
                     /locus_tag="VF309_09015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7466769.1"
                     /translation="MHSRRLLLAALFLAVPLATAQGTAYPTGPIKVIVPFTPGTGADI
                     VARIMQPELGKRLGQPIVVENRPGASGTIAEDHVAKSAPDGYTVLMGADSMVIAPQLY
                     KAVPFHPVNDFQPVSLAARGTLMLVSNPKANIRSLPALIARAKAQPGRIAYGSPGVGT
                     PHHLAMELVCGRTNVDLLHVPYKGTSGFVHDLLGGEVNVGFLPVHIAANLVRDGKLVA
                     LAVGSSRRHPSVPDVPTLAELGYDGMNLDMWFGYFAPAGTSQAVVAKLHDAVSAVLAS
                     PQIREQLARGGLEAAASSPGELHDIARAGYERWGAVIRKNNITAE"
     gene            1724..>3301
                     /locus_tag="VF309_09020"
     CDS             1724..>3301
                     /locus_tag="VF309_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7466770.1"
                     /translation="MKTRRYAVAIVGGGPVGTGLALELGLRGVACALIEPRRALGRIP
                     KGQNLTQRTLEHFSRWGVEREIRAARLMPAGYPIGELTAYGDLMSPYWHAPAGRELVR
                     RFYAQDNERLPQYRTEEVLRRRVGTLACVDFRVGWTARSIRSGDDGAEVAIAGDAGIE
                     ETLAAEYVVGCDGSHSFVRKQAGIERSHEDYEQPMLLAVFRSRSLNEALARFPERSTY
                     RVLRPELKGYWQFFGRVDVPDGWFFHAPVPSGATLESVDALALIQQAAGFPCACEFEH
                     LSLWNMRVAIADRYRAGRVIVAGDAAHSHPPYGGFGLNNGVEDAVNLGWKLAARIEGW
                     GGDALLDSYGLERQPVIRETADDFISARIQRDAAFLERYSPRRDAAEFEAAWRARETD
                     IGSRFQQYEPHYEGSPVIPGPAGGRSGALGQHALRARPGHHLAPLPLAAGGNTFDALG
                     RGFTLLAFDADEGDVPAFEDAARGLRIPLAIVRDTLAGGREAYETRLVLVRPDQYVAW
                     TGDGARVDAGAVLRRATG"
BASE COUNT          530 a         1122 c         1140 g          509 t
ORIGIN      
        1 tatttcgtgg gcgggaaggt ccactcctcg tgggcgctca agacgcgcga gggcatcatc
       61 ctcttcgata cgatctatcc gtacaactcg gaagagctca tcgtcggcgg catgcacaag
      121 ctcgggctcg acccgaagga catcaagtac ttgctggtct cgcatgcgca tcaggatcac
      181 atcggcggtg cggagatgat ccagaaacgc tacgggccgc acgtcgtgat gagcggcccg
      241 gactggaacc tggtcgagaa gctgcccaac cgcttcaaga gcatggcacc caggcgcgac
      301 atggtggcga ccgacgggat gaagatcacg ctcggcgaat cgaccgtgac gctgtggctc
      361 acgccgggcc acacggacgg aaccacgtcc tataccttcc aggtggtcga tcgcggccgt
      421 cccgtgaacg tcgtgtattc cggcggcacc gcgctggtct atgagtggga caagcccgag
      481 tggagcgtga agaacctgca gacgtacatc gattcgcagc gccacctcgc cgcgaaggcg
      541 aaggagacgg gcgccacggt gctgctctcg aaccactccg agttcgacga ggcgtggaag
      601 aagaacagga tgctcgccgg ccgcggcgac ggcccgcatc cctacgagat cggcgcggac
      661 tgggtgcagc gatatttcaa ggtaacggag agctgtgcgc gggcgacgca gctcatggtc
      721 gacaagcgag ccgggcgatc cggataaccg cgtccatgca ctcccgacgc ctgctcctcg
      781 ccgcgctgtt cctcgccgtt cccctcgcaa ccgcccaagg caccgcctac cccacggggc
      841 cgatcaaggt catcgtcccg ttcacgcccg gcaccggcgc cgacatcgtc gcgcgcatca
      901 tgcagcccga gctcggcaag cggctggggc agccgatcgt cgtggagaac cgcccgggcg
      961 cgagcggcac gatcgccgag gaccacgtgg cgaagtccgc tcccgacggc tacaccgtgc
     1021 tgatgggcgc cgacagcatg gtgatcgcgc cccagctcta caaggccgtg ccgttccacc
     1081 ctgtcaacga cttccagccg gtgtcgctcg cggcgcgcgg cacgctgatg ctcgtgtcga
     1141 acccgaaggc gaacatcagg tcgctgcctg cgttgatcgc gcgcgcgaag gcgcagccgg
     1201 gccggatcgc ctacggatcg ccgggcgtgg gcactccgca ccacctcgcg atggagctcg
     1261 tctgcggccg cacgaacgtg gacctgctgc acgtgccgta caaggggacg tccggcttcg
     1321 tccacgacct gctcggcggc gaggtgaacg tgggattcct tccggtgcac attgccgcca
     1381 acctcgtgcg cgacggcaag ctcgtggcac tcgccgtcgg aagctctcgg cgccatccct
     1441 cagtgccgga tgtgccgacg ctcgcggagc tcggttacga cggcatgaac ctggacatgt
     1501 ggttcggata tttcgcgcct gccggaacgt cgcaggccgt cgtcgcgaag ctgcacgatg
     1561 ccgtctccgc ggtgctcgct tcgccgcaga tacgcgagca gctcgcgcgc ggcggcctgg
     1621 aggcggccgc gtcgtcgccc ggcgagctgc acgacatcgc acgcgcgggc tacgagcgct
     1681 ggggcgccgt gatccgcaag aacaacatca ccgcggaatg acggtgaaga cgcgccgtta
     1741 cgccgtcgcc atcgtgggcg ggggaccggt gggcacgggg ctcgccctcg agctcggctt
     1801 gcgcggtgtg gcgtgcgcgc tcatcgagcc ccgcagggcg ctcggacgca tcccgaaggg
     1861 ccagaacctc acgcagcgaa cgctcgagca cttctcgcgc tggggcgtcg agcgcgagat
     1921 tcgcgccgcg cggctgatgc ccgcggggta tcccatcggc gagctgaccg cgtacggcga
     1981 cctcatgagt ccgtattggc acgcgccggc ggggcgcgag ctggtgcgcc ggttctacgc
     2041 gcaggacaac gagcgcctgc cccagtaccg gacggaagag gtccttcgac gcagggtggg
     2101 cacgctcgcc tgcgtggatt tccgcgtcgg ctggaccgcg cgatcgatcc gcagtggcga
     2161 tgacggcgcg gaggtagcga tcgccggcga tgcgggcatc gaggagacgc tcgcggcgga
     2221 atacgtcgtg ggctgcgatg gaagccattc cttcgtgcga aagcaggcgg gcatcgagcg
     2281 cagccatgaa gactacgagc agccgatgct gctcgcggtg ttccgctcga ggtcgctcaa
     2341 cgaggcgctc gcccgttttc ccgagcgctc gacctatcgg gtgctgcgtc cggagctcaa
     2401 gggctattgg caattcttcg gccgcgtcga cgttcccgac ggttggttct tccacgcgcc
     2461 cgtgccttcc ggagccacgc tcgagagcgt cgatgcgctg gccctcatcc agcaggcggc
     2521 gggcttcccg tgtgcgtgcg aattcgagca cctgtcgttg tggaacatgc gggtcgcgat
     2581 cgccgaccga taccgcgcgg gccgcgtcat cgtcgcgggc gacgccgcgc acagccatcc
     2641 gccctacggc ggcttcggcc tgaacaacgg ggtagaagat gccgtcaatc tcggctggaa
     2701 actcgcggcg cggattgaag gatggggcgg tgacgcgctc ctcgattcgt acggtctcga
     2761 gcgccagccc gtcatccgcg agaccgccga cgatttcatc agcgcgcgca tccagcgcga
     2821 cgccgccttc ctcgagcgct acagcccgcg tcgcgatgcc gcggaattcg aagcggcgtg
     2881 gcgtgcgcgc gagacggaca tcggatcgag gttccagcag tacgagccgc attacgaagg
     2941 ctcgcccgtg atccctggcc cggccggcgg gcgcagcggc gcgctcgggc agcacgccct
     3001 ccgcgcgcgg cccgggcacc atctcgcgcc gctgcccctc gccgccggcg gcaatacctt
     3061 cgacgcgctg ggccgcggct tcacgctgct cgcgttcgat gcggatgaag gagacgtccc
     3121 ggcgttcgag gacgcggcgc gcgggcttcg catcccgctc gcgatcgtgc gcgacacatt
     3181 ggcgggcggg cgcgaagcct acgaaacgcg gctcgtgctg gtccgtccgg accaatacgt
     3241 tgcgtggacc ggggatggtg ctcgcgtcga tgcgggtgca gttctgcgcc gcgccacagg
     3301 g
//
LOCUS       DASVII010000692         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_284343,
            whole genome shotgun sequence.
ACCESSION   DASVII010000692 DASVII010000000
VERSION     DASVII010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284343"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VF309_09025"
     CDS             complement(<1..586)
                     /locus_tag="VF309_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7466771.1"
                     /translation="MKMRPAAWSILVASALAWPAAAQETTAAMVAAAEKEGKVVWYSS
                     VDVKVAEGVAKSFREAYPKILVEVERSGSERVFQRINQEYQSNIKNVDVVNSSDASHF
                     IFWKQQKWLAPHTPPDVKRFAAQYKDPEGYFATWRATLSVMAYNTKLVPEADAPKSYA
                     DLLDPKWKGKLTKAHPGYSGTALTGTYALTKALGW"
     gene            complement(616..>1675)
                     /locus_tag="VF309_09030"
     CDS             complement(616..>1675)
                     /locus_tag="VF309_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466772.1"
                     /translation="MAHLELDNLTKHYGTVHSVDGIGLAVEKGEFICLLGPSGCGKTT
                     TLRMIAGFIEPDAGEIRAAGKAISSAREVVPPERRNMSMIFQSYAVWPHMTVRQNVAY
                     GLKMKSVPQAQRDARTDAMLKATKLFGEAARYPSELSGGQQQRVALARALAPNPDILL
                     LDEPLSNLDANLRGDMRLEIRRLHDEFHTTSIYVTHDQVEAMTMADRIVVMNAGRIEQ
                     IGTPEEVYDRPNSRFVARFLGGSNVIEGVHVAGRRVDVAGHPLEVAEGELAGPGRETG
                     VCVKTHDVELLTDTRADGGNTIPGVVRSHSFLGSHRDYVVDIGQDIFVAAPAALVVPA
                     GAKVRVKFRPDKCRGLVR"
BASE COUNT          240 a          556 c          572 g          307 t
ORIGIN      
        1 cccagccgag cgccttggtg agcgcgtagg tgccggtcaa cgccgtgccg ctgtatcccg
       61 ggtgcgcctt ggtgagcttg cccttccact tgggatcgag gaggtcggcg taggacttgg
      121 gcgcatcggc ctccgggacg agtttcgtgt tgtacgccat caccgagagt gttgcgcgcc
      181 acgtcgcgaa gtagccctcg ggatccttgt attgcgcggc gaagcgcttc acgtccggtg
      241 gcgtgtgggg cgcgagccac ttctgctgct tccagaagat gaagtgcgac gcgtcggacg
      301 agttcaccac gtccacgttc ttgatgttcg actggtactc ctggttgatg cgctggaaga
      361 cgcgctcgga gccgctacgc tcgacttcga cgaggatctt cggatacgcc tcgcggaagg
      421 acttggcgac gccctcggcg actttcacgt cgaccgacga gtaccacacg accttgccct
      481 ccttctcggc ggcggcgacc atcgccgcgg tggtctcctg cgcggcggcg ggccaggcga
      541 gcgcgctcgc cacgagaatg ctccatgcag ccggtctcat cttcatctgt ctctcctcga
      601 tttcgttgtc catgcctacc gtaccagccc ccggcacttg tccgggcgaa acttcacgcg
      661 caccttcgcg cccgcgggca ccacgagcgc cgccggggcc gcgacgaaaa tatcctggcc
      721 gatgtcgacc acgtagtcgc gatggctgcc gaggaaggag tggctgcgaa cgactcccgg
      781 gatggtgttg ccgccgtcgg cccgcgtatc ggtgaggagc tcgacgtcgt gggtcttcac
      841 gcacacgccg gtctcgcggc cgggcccggc gagctcgccc tccgcgacct cgagcgggtg
      901 cccggcgaca tcgacgcggc ggccggcgac gtgcactccc tcgatcacgt tgctgccgcc
      961 gaggaaacgc gcgacgaagc gcgagttggg ccggtcgtag acctcctcgg gcgttccgat
     1021 ctgctcgatg cgcccggcgt tcatgacgac gatgcggtcg gccatggtca tcgcttccac
     1081 ctgatcgtgc gtcacgtaga tggaggtggt gtggaattca tcgtgaaggc gccggatctc
     1141 gaggcgcatg tcgccgcgca ggttggcgtc gagattcgac agcggctcgt cgaggaggag
     1201 gatgtccggg ttcggcgcga gcgcgcgcgc gagcgcgacc cgctgctgct gcccgccgga
     1261 gagctccgag ggatagcgcg ccgcctcgcc gaagagcttg gtcgccttga gcatcgcgtc
     1321 ggtgcgggcg tcgcgctgcg cctgcggcac cgacttcatc ttcaggccgt acgccacgtt
     1381 ctggcgcacc gtcatgtgcg gccacaccgc gtagctctgg aagatcatgc tcatgttgcg
     1441 ccgctcgggc ggaacgacct cgcgcgccga ggagatcgcc ttgccggcgg cgcgaatctc
     1501 gcccgcgtcg ggctcgatga agcccgcgat catcctgagc gtcgtcgtct tcccgcagcc
     1561 cgaggggccg aggaggcaga tgaactcgcc cttctcgacc gcgaggccga tgccgtcgac
     1621 ggagtggacc gttccgtagt gcttcgtcag attgtcgagc tcgaggtggg ccata
//
LOCUS       DASVII010000693         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_356156,
            whole genome shotgun sequence.
ACCESSION   DASVII010000693 DASVII010000000
VERSION     DASVII010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356156"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VF309_09035"
     CDS             complement(<1..705)
                     /locus_tag="VF309_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX7466773.1"
                     /translation="MIVTPAQVEEVARELYVRALKILPPDIKQGFARLAASETDPTAR
                     KVLGTMIANITVAEDTENMLCQDTGIPIYNVTIGRGVEIAGTELRAAIRRGCERATRE
                     HPLRSSVVHPMTRRNEHTSCGRGVPVIHIDFSDIDETLEIEMIPKGSGSENSSWLKMA
                     IPAEGVDAIKTFVVDCVLESGGKSCPPTIVGVGVGGTADLCVHLAKQAATRPLGSRCA
                     DPEGAELEQALSAAVNQ"
     gene            complement(705..1673)
                     /locus_tag="VF309_09040"
     CDS             complement(705..1673)
                     /locus_tag="VF309_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7466774.1"
                     /translation="MVRLITRLAAALLAALLPAAALAQAWPSKPVKIVVPFAAGGATD
                     VVARLLAQKLTEVWGQSVVVENRVGAGGNIGGDAVAKSPADGYTLLMASGAIVIAGPH
                     MYKSLPYDPARDLVAITNVATGPQVIAVTNSLPVKDLGELIAYAKANPGKLNYGSAGV
                     GAQTHLAAENLAQAAGIELTHVPYKGESAALTDLMGGQIQLVVANLSGAIALVREGRI
                     RALAVTSRERNAQLPDVPAAAETLPGFENSGWFGLLAPTGTPRDVIDKVYHDSAKIAL
                     SDEFRAKLAQLGMVPVANSPADFSASNRRESARWERVIRERGIVAN"
BASE COUNT          260 a          613 c          628 g          308 t
ORIGIN      
        1 ctggttcacc gccgcggaca gcgcctgctc cagttcggcg ccctcggggt cggcgcagcg
       61 cgagcccagc ggccgcgtcg ccgcctgctt ggccaggtgc acgcacaggt cggcggtgcc
      121 gcccacgccc acgccgacga tggtgggcgg gcagctcttg ccgcccgact ccagcacgca
      181 atcgacgacg aaggtcttga tcgcatccac gccctcggca gggatggcca ttttcagcca
      241 ggagctgttc tccgagcccg aacccttcgg gatcatctcg atctcgagcg tctcgtcgat
      301 gtcggaaaaa tcgatgtgga tcaccggtac gccgcggccg caggaagtat gttcgttcct
      361 gcgcgtcatc gggtgcacca ccgaggatcg cagcggatgc tcgcgcgtgg cgcgctcgca
      421 gccgcggcgg atcgcggccc gcagctcggt gccggcgatc tcgacgccgc gcccgatggt
      481 gacgttgtag atcgggatgc cggtgtcctg gcagagcatg ttctcggtgt cttccgcgac
      541 cgtgatgttg gcgatcatgg tgccgaggac cttgcgcgcc gtgggatcgg tctcgctcgc
      601 cgcgagccgc gcgaatccct gcttgatgtc gggcggcagg atcttcagcg cgcgcacgta
      661 gagctcgcgc gccacctcct cgacttgtgc gggcgtgacg atcatcagtt ggccacgatg
      721 cctcgttcgc ggatcacgcg ctcccagcgc gcgctttccc tgcggttcga ggcgctgaaa
      781 tcggcgggcg agttcgccac gggcaccatg ccgagctgcg cgagcttggc gcggaattcg
      841 tccgacagcg cgatcttcgc gctgtcgtga tagaccttgt cgatcacgtc cctgggcgtt
      901 cccgtggggg cgaggaggcc gaaccacccg gaattctcga atcccgggag agtttcggcg
      961 gcggcgggaa cgtcgggaag ctgcgcgttc ctttcccggc tggtgacggc aagcgcgcgg
     1021 atccttccct cccgcaccag cgcgatcgcg ccgctcaggt tcgcgacgac gagttggatc
     1081 tgcccgccca tgaggtcggt gagggcggcc gattcccctt tgtacggaac gtgcgtgagc
     1141 tcgatcccgg cggcttgcgc gagattctcg gcggcgaggt gggtctgcgc gccaacgccc
     1201 gcgctgccgt agttcaactt tcccgggttg gccttcgcat aggcgatgag ctcgcccagg
     1261 tccttcacag gcaggctgtt ggtgaccgcg atcacctgcg ggccggtggc gacgttggtg
     1321 atggcgacca ggtcgcgcgc cggatcgtag ggcagcgact tgtacatgtg cggcccggcg
     1381 atcacgatcg cgcccgaggc catgagcagt gtgtagccat cggcaggcga cttggcgacc
     1441 gcgtcgccgc cgatgttgcc gcccgcgccg acgcgattct cgaccactac cgattggccc
     1501 cacacctccg tgagcttctg cgcgaggagg cgcgccacca cgtcggtcgc gccccccgcg
     1561 gcgaacggca cgacgatctt caccggtttc gacggccagg cctgcgcgag cgccgccgcg
     1621 ggcaggaggg cggcgagcag tgccgcggca aggcgcgtga tgagtcgaac catggttccc
     1681 ttctcccagc gagcgatcag gatagctctc atcgggcgag cgcgaacgcg aggccgcaaa
     1741 cgagcgcgaa tgccgcggcg agcgcggcga gcgtcttctg ccactcgcgc cacggcagga
     1801 attcaagcg
//
LOCUS       DASVII010000694         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_33184, whole
            genome shotgun sequence.
ACCESSION   DASVII010000694 DASVII010000000
VERSION     DASVII010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33184"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..>1614)
                     /locus_tag="VF309_09045"
     CDS             complement(122..>1614)
                     /locus_tag="VF309_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7466775.1"
                     /translation="AQQTAPVANFSGGWRMRLNLAQALMCRSDLLLLDEPTNHLDLDA
                     VLWLEDWLGAFPGAVILITHDREFLDAVVGGIVHVENRQLNAYAGNYSAFETQRAARL
                     GQQQAAYEKQQRVVAHLEAFITRFRAKATKARQAQSRIRALEKLERIAAAHVDSPFYF
                     AFKAPAEKPRQLFRLEDVSVGYGGAPVLSGVEWSVLPGDAIGLLGPNGAGKSTLLKAI
                     VGDLVPTAGEIHRAQGLRIGYFAQHQVDQLRLDESALWHLARLAPEEREQSFRDFLGG
                     FDFRGDQVSQKVASLSGGEKARLALALIVWQRPNLLLLDEPTNHLDIEMREALAQALT
                     DFEGALIVVAHDRHLLSAATDQWMLVADGKVAPFEGDLDDYKQWAREYHARGTRREGR
                     ETPSVNRREERRSEASVRQREAAARKPFEKRLAAIEAELAPLARESTEAEAWLALPEA
                     YEETNRERMQATLKRRAELAGRISGLEDDWLWTQAEIEKELDKLKG"
BASE COUNT          229 a          572 c          545 g          268 t
ORIGIN      
        1 gcactaaacg gccccggtgt tccacttgca acgcggcagg cctcgggccg gggtcgccgc
       61 aaggtgccac tttccgcatc ggggcgtcac atcggcttcg aggagcgccc cgacgacccg
      121 ctcacccctt gagcttgtcg agctccttct cgatctcggc ttgcgtccag agccaatcgt
      181 cctcgagccc cgagatgcgg ccggcgagct cggcgcggcg cttgagcgtc gcctgcatgc
      241 gctcgcgatt ggtctcttca taggcctccg gcaaggcgag ccacgcctcg gcctcggtgc
      301 tttcgcgcgc caggggcgcg agctccgcct cgatcgccgc gaggcgcttc tcgaagggct
      361 tgcgcgccgc cgcttcgcgc tgccgcacgg aggcttcgct gcgccgctct tccctgcgat
      421 tgaccgaagg cgtctcccgc ccctcgcgcc tcgtgcctcg ggcatggtat tcgcgcgccc
      481 actgcttgta gtcgtcgagg tcgccttcga acggcgcgac ctttccgtcc gcgaccagca
      541 tccattgatc cgtcgccgcg gagagcaggt ggcgatcgtg ggcgaccacg atgagggcgc
      601 cttcgaagtc ggtgagcgcc tgcgcgagcg cttcgcgcat ctcgatgtcg aggtggttcg
      661 tcggctcgtc gagcaggagg agattgggcc gctgccagac gatcagcgcg agcgcgaggc
      721 gcgccttctc gccgcccgac aggctcgcca ccttctgcga gacctggtcg ccgcgaaaat
      781 cgaagccgcc gagaaaatcg cggaatgact gctcacgctc ctccggcgcg aggcgcgcga
      841 ggtgccagag cgcgctctcg tccaggcgca gctggtcgac ctggtgctgc gcgaagtagc
      901 cgatgcgcag accctgcgcg cgatggatct cgcccgccgt cggcacgagg tccccgacga
      961 tcgccttcag cagcgtcgac ttgcccgcgc cgttcgggcc gagaaggccg atcgcgtcgc
     1021 cgggcagcac gctccactcg acgccgctca gcaccggagc gccgccgtat ccgaccgaga
     1081 cgtcctcgag gcggaagagc tggcgcggct tttccgccgg cgccttgaac gcgaaataga
     1141 agggcgagtc gacgtgggcg gcggcgatgc gctcgagctt ttccagcgcg cggatgcggc
     1201 tctgcgcctg gcgcgccttg gtggccttcg cgcgaaagcg cgtgatgaac gcctcgaggt
     1261 gcgcgacgac gcgctgctgc ttctcgtaag cggcctgctg ctgtccgagc cgcgcggcgc
     1321 gctgcgtctc gaaggcgctg tagttccccg cgtacgcatt gagctggcgg ttctcgacat
     1381 gcacgatgcc gcccaccacc gcatcgagaa actcgcggtc gtgggtgatg aggatcaccg
     1441 ctccgggaaa cgccccgagc cagtcttcga gccacagcac cgcgtcgagg tcgaggtggt
     1501 tggtcggctc gtcgagcagc agcaagtccg accggcacat gagcgcctgc gccagattga
     1561 gccgcattcg ccagccgccg gaaaaattcg ccaccggcgc ggtttgctgc gcct
//
LOCUS       DASVII010000695         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_535801,
            whole genome shotgun sequence.
ACCESSION   DASVII010000695 DASVII010000000
VERSION     DASVII010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535801"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1693
                     /locus_tag="VF309_09050"
     CDS             <1..>1693
                     /locus_tag="VF309_09050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507895.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7466776.1"
                     /translation="NPVFIMVDSKARGSRQQIRQLAGMRGLMARPSGEIIETPIQSNF
                     REGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVVIEDDCGTTSGMA
                     MKALVEGGEVVEALRERILGRVVAADVFNTENQEILLAAGTLLDEDAVERIESLGIDE
                     VKVRTPLTCDTRYGICAACYGRDLGRGARVNVGEAIGVIAAQSIGEPGTQLTMRTFHI
                     GGAASRTAVASSVESKSNGVAHFAPTMRRLTNSRKEEVAISRNGEIIISDETGRERER
                     HKVPYGATLTIDDGAKVKAGQSLANWDPLTRPIITEYAGETKFENVEEGVTVAKQIDE
                     TTGLSSLVVIDPKRRGTSATKGLRPAVKLIGDKGKEMKLAGGDTPVNYTFQIGAIITV
                     KNGQQVNVGDVLARIPQESSKTRDITGGLPRVAELFEARSPKDAGLLAQVTGTVSFGK
                     DTKGKQRLVITDFDGTAHEYLIPKDKHITVHDGQVVNKGEMIVDGPADPHDILHLQGM
                     EALARYIIDEVQDVYRLQGVKINDKHIEVIVRQMLRRVSIVDQGETRFITGEQVER"
BASE COUNT          331 a          550 c          571 g          241 t
ORIGIN      
        1 ttaacccggt atttatcatg gtggacagca aagcccgtgg ttcgcgccag cagattcgcc
       61 agctggccgg catgcgcggg ttgatggctc gcccctcggg cgaaatcatc gagaccccga
      121 ttcagtcgaa cttccgcgaa ggcttaaacg tgctgcagta cttcatctcg acgcacggcg
      181 cgcgcaaagg cctggcggat accgcgttga agacggcgaa ctcgggctat ctcacgcggc
      241 gtctggtcga cgtcacgcag gacctggtgg tcatcgagga cgattgcggc accacttccg
      301 gcatggcgat gaaggcgctg gtcgagggcg gcgaagtcgt ggaggcgctg cgcgagcgca
      361 tcctcgggcg agtcgtcgcg gccgacgtgt tcaataccga gaaccaggaa atcctgcttg
      421 cggcgggcac gctcctcgac gaggacgcgg tcgaacgcat cgagagcctg ggcatcgacg
      481 aggtcaaggt gcgcacgccg ctcacctgcg acacgcgcta tggcatctgc gcggcctgct
      541 acggccgcga cctgggccgt ggagcgcgcg tgaacgtggg cgaggcgatc ggcgtcatcg
      601 ccgcgcagtc gatcggcgag ccgggcacgc agctcacgat gcggaccttc cacatcggcg
      661 gcgcggcttc gcgcaccgcg gtggcgagct ccgtggagtc gaagtccaac ggtgtcgcgc
      721 acttcgcgcc caccatgcgt cgcctcacca actcccgcaa ggaggaagtg gcgatctcgc
      781 gcaacggcga gatcatcatc tccgacgaga cgggccgcga gcgcgagcgc cacaaggttc
      841 cgtacggcgc gacgctcacg atcgacgacg gcgccaaggt gaaggcgggg cagtcgctcg
      901 ccaactggga tccgctcacg cgccccatca tcaccgagta cgccggcgag acgaagttcg
      961 agaacgtcga agagggcgtc acggtggcca agcagatcga cgagaccacg ggcctttcga
     1021 gcctggtcgt gatcgatccg aagcgccgcg ggacctccgc cacgaagggc ttgcgccctg
     1081 ccgtgaagct gatcggcgac aagggcaagg agatgaagct cgccggaggc gacacgccgg
     1141 tgaactacac cttccagatc ggcgcgatca tcaccgtgaa gaacggccag caggtgaacg
     1201 tgggcgacgt gctcgcgagg atcccgcagg aatcgtccaa gacgcgcgac atcacgggcg
     1261 gcctgccgcg cgtggcggag ctcttcgagg cgcgttctcc caaggacgcg ggcctcctcg
     1321 cgcaggtcac cgggaccgtc tccttcggca aggacaccaa gggcaagcag cgcctggtga
     1381 tcaccgactt cgacggcacc gcccacgagt acctgatccc gaaggacaag cacatcaccg
     1441 tgcacgacgg gcaggtggtc aacaagggcg agatgatcgt cgacggcccg gccgacccgc
     1501 acgacatcct tcacctccag gggatggaag cgctcgcgcg ctacatcatc gacgaggtgc
     1561 aggacgtcta ccggctccag ggcgtgaaga tcaacgacaa gcacatcgag gtgatcgtgc
     1621 gccagatgct gcgccgcgtc tcgatcgtcg atcagggcga aacccgcttc atcacgggcg
     1681 agcaggtcga gcg
//
LOCUS       DASVII010000696         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_356220,
            whole genome shotgun sequence.
ACCESSION   DASVII010000696 DASVII010000000
VERSION     DASVII010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356220"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VF309_09055"
     CDS             <1..825
                     /locus_tag="VF309_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7466777.1"
                     /translation="LIHSGSSRADAPFVAVNCGAIPENLMESEFFGYKKGAFTGADTD
                     KMGYLKAAEKGTLFLDEVGDLPLGMQVKLLRVIQEKKVRRVGDTVEDPIDVRIISATH
                     RNLSEQVDAGQFRQDLFYRLNVIEMKMPGLREIPEDIALMVQQVLERLARQSGNPPPA
                     IAEEAMLALRRYDFPGNVRELENILERALALCSDNKILESDLYLTQASSKDRLPPLDG
                     ALGTRGLPLHDFLDQIEREAITKALEATRFNKTAAAKLLGITFRSLRYRLDRLGID"
     gene            878..1405
                     /gene="ampD"
                     /locus_tag="VF309_09060"
     CDS             878..1405
                     /gene="ampD"
                     /locus_tag="VF309_09060"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEX7466778.1"
                     /translation="MTGNSSLTPVFPESPFCDERPEGEKITLIVIHNIALPPGEYGGP
                     WIDDLFMGRLDPKAHPYFEEIAGLEVSSHYLIRRDGALTQYVPCEKRAWHAGVSSWKG
                     RDRCNDFSIGIELEGSDDVPFSEPQYETLIALTRELFSCYGKLDLAGHSDVAPGRKTD
                     PGPWFEWHRFRAAVT"
     gene            1706..>2642
                     /locus_tag="VF309_09065"
     CDS             1706..>2642
                     /locus_tag="VF309_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX7466779.1"
                     /translation="MRLASDIHPTAPLANPILGEEGTTPQERYADYKIIRRNGAVVGF
                     EPDKIAVAMTKAFLAVSGSTAAASAGMREKVQTLTEAVVQSLMRRHPAGGTFHIEDVQ
                     DQVELAMMRAGEHQVARSYVLYREERARERAKRVQEAVESRQPRIHVMVGGAKEPLDT
                     HKLEVLITSACEHLGADVDPKKIMDATLRDLYDGVPLEEVHKCAILAARMLLEQDPDY
                     TYVTARLLLHTIRREVLGEEVLQAEMATRYAEYFPTFISQGVKAQLLDERLASFDLKR
                     LGAALKPDRDLQFNYLGLQTLYDRYFLHINEKRIEL"
BASE COUNT          513 a          887 c          804 g          438 t
ORIGIN      
        1 ctcatccatt cgggatcctc ccgcgcggat gctccatttg tcgcggtgaa ctgcggcgcg
       61 attcccgaga acctgatgga gagcgagttc ttcggctaca agaagggggc gttcaccggc
      121 gccgacacgg acaagatggg atatctcaag gcggcggaga agggcacgct cttcctggac
      181 gaggtcggcg acctaccgct cgggatgcag gtgaagctgc tgcgcgtgat ccaggagaag
      241 aaggtgcggc gcgtcggcga cacggtggaa gatccgatcg acgtgcgcat catctcggcc
      301 acccaccgca acctctcgga gcaggtcgac gccggccagt tccgccagga tctcttctat
      361 cggctcaacg tgatcgagat gaagatgccg ggccttcgcg agattccgga ggacatcgcg
      421 ctcatggtgc agcaggtgct cgagcgcctc gcgcgccaga gcggcaatcc gccgccggcg
      481 atcgccgagg aggcgatgct cgcgctgcgc cgctacgatt tccccggcaa cgtgcgcgag
      541 ctggagaaca tcctcgagcg cgccctcgcg ctttgcagcg acaacaagat actcgagagc
      601 gatctctacc tcacccaggc gagctccaag gaccggctcc cgcccctcga cggcgcgctc
      661 ggcactcgcg gcctgccgct gcacgacttc ctcgaccaga tcgagcgcga agccatcacc
      721 aaggccctcg aagccacgcg cttcaacaag accgccgccg cgaagctcct cggcatcacc
      781 ttccgctccc tgcgctatcg attggatcga ctggggatcg attgatggaa aaactggtgc
      841 cagagacgag tttccgccgg accgcggtgt gccgtggatg actggaaact cgtctctgac
      901 accagttttt ccagagtctc ctttctgcga cgagcgcccg gaaggcgaga agatcacgct
      961 catcgtgatc cacaacatcg cgctgccgcc gggagaatac ggcgggccgt ggatcgacga
     1021 tctcttcatg ggccgcctgg acccgaaggc gcatccctat ttcgaggaga tcgcgggact
     1081 cgaagtgtcc tcgcactatt tgatccgccg cgacggcgcg ctcacgcagt acgtgccgtg
     1141 cgagaagcgc gcgtggcacg cgggagtctc gagctggaag gggcgcgatc gctgcaacga
     1201 tttttccatc ggcatcgagc tcgagggctc cgacgacgtg cccttctccg agccgcagta
     1261 cgagacgctc atcgcgctca cccgcgagct cttctcgtgc tacggcaagc tcgatctcgc
     1321 cggccatagc gacgtggccc cgggccgcaa gaccgatccc ggcccgtggt tcgagtggca
     1381 ccgtttcagg gccgctgtca cgtaactgtt actgctcaat tcttcagcaa gaaccccttg
     1441 caagagcctg aatccgcgag agaaaaaaga cttgaaaaag tctcttgcgt tggccattca
     1501 cccttactac actttgtgtc catgccgttc cggcagcact atatcttgtg gaatggttgt
     1561 gaataacgtg tgcaaaagcc ggtcgacgcg ggcgagttgt tccagcgaat ccggtgctta
     1621 ggcaggccaa gaacctgcgg tagtgaaaat ccggggcgac ggcgcccgac gccccggacc
     1681 ctaaaacggc atcaggagaa gacccatgcg cttggcatcc gatatccatc ccacggcacc
     1741 tctcgcgaat ccgattctcg gagaggaagg cactacgccg caggagcgat acgcggacta
     1801 caagatcatc cgtcgcaacg gcgccgtcgt cggcttcgag cccgacaaga tcgcggtcgc
     1861 gatgaccaag gcgttcctcg ccgtctccgg cagcaccgcc gccgcgagcg ccggcatgcg
     1921 cgagaaggtc cagacgctca ccgaggccgt ggtgcagtcg ctcatgcgcc gccatcccgc
     1981 gggcggcacc ttccatatcg aggacgtgca ggaccaggtc gagctcgcga tgatgcgcgc
     2041 cggcgagcac caggtcgcgc gttcctacgt gctctatcgc gaggagcgcg cgcgcgaacg
     2101 cgccaagcgc gtccaggaag cggtcgagtc gcgccagccg cgcatccacg tgatggtggg
     2161 aggcgcgaag gagccgctcg atacgcacaa gctcgaagtg ctcatcacct cggcgtgcga
     2221 gcacctcggc gccgacgtcg atcccaagaa gatcatggac gccacgctgc gcgatctcta
     2281 cgacggcgtc cccctcgagg aagtgcacaa gtgcgcgatc ctcgccgcgc gcatgctcct
     2341 cgagcaggac cccgactaca cctacgtcac cgcgcgcctc ttgctgcaca cgatccgccg
     2401 cgaagtcctc ggcgaggaag tgctgcaagc ggagatggcg acgcgctacg ccgagtactt
     2461 ccccactttc atcagccagg gcgtgaaggc gcagctcctc gacgagcgcc tcgcctcctt
     2521 cgacctgaag cgcctcggcg ccgcactgaa gcccgatcgc gacctgcagt tcaactacct
     2581 cgggctgcag acgctctacg accgctactt cctgcacatc aacgagaagc gcatcgaatt
     2641 gc
//
LOCUS       DASVII010000697         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_535891,
            whole genome shotgun sequence.
ACCESSION   DASVII010000697 DASVII010000000
VERSION     DASVII010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535891"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VF309_09070"
     CDS             <1..242
                     /locus_tag="VF309_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466780.1"
                     /translation="AFTQCLLGKYAQCRADFVKIYDVDPKFDLTPAEAVHPSWTKTFA
                     AAKAQAKKALEEKEAREAKERAKGPPPTASVPKKP"
     gene            250..1602
                     /locus_tag="VF309_09075"
     CDS             250..1602
                     /locus_tag="VF309_09075"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7466781.1"
                     /translation="MIATLGRYKIVSEIGQGAMGVVYKAVDPIIDRTVAIKTINLNLS
                     RQELEEYEARFQQEIKAAGRLNHPNIVTIYDVGKTDQVAYMAMEFLEGQELKDMIAMG
                     QLPPADVIVDIIAQVADGLSFAHSQDIVHRDVKPSNIMVMKGTLAKITDFGIARLPNS
                     AVKTMTGLILGSPRYMSPEQVIGKAIDSRSDIFSLGVVLYEALTGVAPFDGDNVNAIM
                     YATVNTTPPPPSAHNRAVPPMLDLIVAKAMAKLLEDRYQAVKELGDDLREVRRQMDHS
                     KPAAALKAMTSPVTRPVVKDLADTDTIPMSPGKGTDDDTKPLALNKVFDSFDATMRLA
                     SMTDQVDDFKEYITATQKMRAYKGKIEPGSPLAPKAPAPAAAPAPAPAPAPAPAPAPV
                     PAPVAMPLPVEKKPAPRPIAIPSMQSSVSASNSNLLAVAAIAVLSLAAIGLIIALLLK
                     "
     gene            complement(1669..>2013)
                     /locus_tag="VF309_09080"
     CDS             complement(1669..>2013)
                     /locus_tag="VF309_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX7466782.1"
                     /translation="LHRHAGIACFDQDVFLNAVGGVRIVEPGADLAVTLAIVSSLRNR
                     ALPDKHVVFGEVGLAGEVRPVQRGQERLREAAKLGFTHAIIPAANKPRQAIPGLEVIA
                     VNRVVEAVDYLK"
BASE COUNT          381 a          714 c          615 g          303 t
ORIGIN      
        1 tcgccttcac gcaatgcctg ctcgggaagt acgcgcagtg ccgcgccgac ttcgtcaaga
       61 tctacgacgt cgatcccaaa ttcgacctca cgccggccga ggcggtacac ccctcgtgga
      121 cgaagacctt cgcggccgcc aaggcccagg ccaagaaagc gctggaggag aaggaagccc
      181 gggaggcgaa ggagcgcgcg aaggggccgc cgcccacggc ctctgtgccg aagaaaccct
      241 agccgctcca tgatcgcgac gctcggacgc tacaagatcg tttctgaaat cggccagggg
      301 gcgatgggtg tcgtctacaa ggccgtggat ccgatcatcg accgcacggt cgcgatcaag
      361 accatcaacc tcaacctctc gcgccaggag ctcgaggagt atgaggcgcg cttccagcag
      421 gagatcaagg ccgcggggcg cctgaaccac ccgaacatcg tcaccatcta cgacgtcggc
      481 aagaccgacc aggtcgcgta catggcgatg gagttcctgg agggccagga gctgaaggac
      541 atgatcgcca tggggcagct gccccccgcc gacgtgatcg tggacatcat cgcgcaggtg
      601 gccgacgggc tttcgttcgc gcactcgcag gacatcgtgc accgcgacgt gaagccctcc
      661 aacatcatgg tgatgaaggg cacgctcgcg aagatcaccg acttcggcat cgcgcggctg
      721 cccaattccg cggtgaagac catgacgggg ctgatcctcg gctcaccccg ctacatgtcg
      781 ccggagcagg tgatcggcaa ggccatcgac tcgcgctcgg atatcttttc cctgggggtg
      841 gtgctgtacg aggccctcac cggcgtggcg cccttcgacg gcgacaacgt gaacgcgatc
      901 atgtacgcga cggtgaacac gacgcccccg ccgccctccg cgcacaatcg cgccgtgcca
      961 ccgatgctcg acctcatcgt cgcgaaggcg atggcgaagc tgctggagga ccgctaccaa
     1021 gcggtcaagg aattgggcga cgacctgcgc gaagtgcgcc ggcagatgga ccactcgaag
     1081 cccgccgcgg ccctgaaggc gatgacatcg ccggtgaccc gtcccgtcgt gaaggatctc
     1141 gccgacaccg atacgatccc catgtcgccc ggcaagggaa ccgacgacga cacgaagccg
     1201 ctcgcgttga acaaggtttt cgattccttc gacgcgacca tgcgcctcgc ctcgatgacc
     1261 gaccaggtcg acgatttcaa ggaatacatc accgcgacgc agaagatgcg cgcctacaag
     1321 ggcaagatcg agcccggctc gccgcttgcc cccaaggccc cggcgccggc ggccgcgccc
     1381 gcccctgcac ctgcacctgc gcccgcaccc gcacccgctc cggtgcccgc gccggtcgcg
     1441 atgccgctgc ccgtcgagaa gaaacccgcg ccgcgtccga tcgccattcc ctcgatgcaa
     1501 tcgagcgtgt ccgcgagcaa ctcgaacctg ctggccgtcg ccgcgatcgc ggtgctctcc
     1561 ctcgccgcca tcggcctcat catcgccctg ctgctgaaat agaaactggt gtcagagacc
     1621 aatttccccg gtacgcatgc cggagaaatc ggtctctgac accagtttct acttcaagta
     1681 gtccaccgcc tccaccaccc gattcacggc gatcacctcg aggcccggga tggcctgccg
     1741 gggcttgttg gccgcgggaa tgatggcgtg ggtgaagcct agcttcgcgg cttcccgcag
     1801 gcgctcctgg ccgcgctgca cgggcctgac ctcgcccgcg agccccacct cgccgaacac
     1861 cacgtgcttg tcgggcagcg cgcggttgcg aaggctcgat acgatcgcca gcgtcaccgc
     1921 gagatccgct ccgggctcga cgatgcgcac gccccccacc gcgttgagga acacatcctg
     1981 gtcgaagcac gcgatgcctg cgtgccgatg cag
//
LOCUS       DASVII010000698         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_679854,
            whole genome shotgun sequence.
ACCESSION   DASVII010000698 DASVII010000000
VERSION     DASVII010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679854"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VF309_09085"
     CDS             <1..488
                     /locus_tag="VF309_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466783.1"
                     /translation="GHVEFDLANVRVVPLIADLENVVGPQLRAASLTLLQAPCDTRLA
                     VYGDEEKIRQIMLNLLSNAIKFSFAGGTISIECTEAESRVRIAVRDTGIGIAPERLDA
                     VFDPFVQLDRTLASRHEGTGLGLSISRDLARAMGGDISAVSQPGKGSTFTLTLPRAGK
                     V"
     gene            485..916
                     /locus_tag="VF309_09090"
     CDS             485..916
                     /locus_tag="VF309_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX7466784.1"
                     /translation="MSAARKRTTATDPLSRLRTLCLALPEAHEVVAWNEPTFRVRNKI
                     FAMYASAASHHGKGRAGVWCNCMPMEQDLLLHADPDRFFKPPYVGPKGWIGVYLDRRP
                     RWTAVADIVQEAYRLTAPSRLVAQLDAPRAPAARSPRTHSR"
     gene            913..1824
                     /locus_tag="VF309_09095"
     CDS             913..1824
                     /locus_tag="VF309_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7466785.1"
                     /translation="MNQATPPVPLADATRLGLVRLQIADLPRSLAYYQETLGFQLLER
                     GTDWATLGTVDGTPLVQLVARAGARPAPAQGRLGLYHFAILLPTRASLGRLIGHLAER
                     RERFGASDHLVSEALYLRDPDGLGIEVYVDRPRSSWRRENGQVAMDTAPLDLESVVRD
                     SAGARWTGMPNGTQVGHVHLHVGDLAIASDFYHAALGFERTVWSYPGALFLAAGGYHH
                     HLGLNTWAGAGAANPRDDDARLLEWTIVLPSAEQVRETAARLERTAHSVERDAHGARA
                     IDPWGTRFRIADEESYARLTARGGASR"
     gene            1835..>2198
                     /locus_tag="VF309_09100"
     CDS             1835..>2198
                     /locus_tag="VF309_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466786.1"
                     /translation="MLSLSSLAGWEESVLSSIAGANGTVEERDRQIERSGMYGEYPAI
                     VRAYIELFGDDDSALEALKRALFLVWQSAVALPADTGLAALPEGTARLVVEQLDDAIR
                     RGGDDAELRWMLAWYHAHG"
BASE COUNT          340 a          776 c          732 g          350 t
ORIGIN      
        1 ccggccacgt cgagttcgat ctcgccaacg tgcgtgtcgt acccctcatc gccgatctcg
       61 agaatgtcgt gggcccgcaa ctgcgggccg cgtcgctcac actgctgcag gcgccgtgcg
      121 atacgcggct cgccgtgtac ggagacgaag agaagatccg gcagatcatg ctcaatctgc
      181 tctccaacgc catcaagttc agcttcgccg gcggcaccat cagcatcgag tgcacggaag
      241 ccgaatcgcg tgttcgcatt gccgttcgcg acacgggcat cggcatcgcc cccgagcggc
      301 tggacgcggt gttcgatcca ttcgtgcagc tcgaccgcac tctcgcgagc cgccacgaag
      361 gcaccgggct cgggctctcg atcagccgcg acctcgcgcg tgccatgggc ggcgacatca
      421 gcgcggtgag ccaaccgggc aagggctcga catttaccct cactcttccg cgcgccggaa
      481 aagtatgagc gcagcccgca agcgcaccac cgccaccgac ccgctctctc gcctgagaac
      541 gctctgtctc gccctccccg aggcacacga agtggtggcg tggaacgagc ccacctttcg
      601 cgtgcgcaac aagatcttcg ccatgtacgc aagcgcggcg tcgcatcacg gaaagggacg
      661 cgccggcgtc tggtgcaact gcatgcccat ggagcaggac ctgctgctgc atgccgatcc
      721 ggatcgtttc ttcaagccgc cgtacgtggg acccaaaggt tggatcggcg tatatctcga
      781 ccggcgtcct cggtggaccg cggtggcgga tatcgtgcaa gaggcctacc ggctcaccgc
      841 gcccagccgg ctcgtcgcgc agctcgacgc gccgcgcgcg cctgccgcgc gctcacctcg
      901 cacgcactca cgatgaacca ggcaacgccg cccgtcccac tcgccgacgc gaccaggctc
      961 ggcctcgtcc ggctgcagat cgccgacctg ccacggtcgc tcgcgtacta ccaggagaca
     1021 ctcggcttcc agctcctcga gcgcggcacg gactgggcca ccctcggcac cgtcgatggg
     1081 acgccgttgg tgcagctcgt ggcgcgcgcc ggcgcgcggc ccgcgcccgc gcaggggagg
     1141 ctcggactct atcactttgc catcctgctg cccacgcgcg cgtcgctcgg ccgcctcatc
     1201 ggacatctcg ccgaacggcg cgagcgcttc ggtgcatcgg atcatctcgt gagcgaggcg
     1261 ctctatcttc gcgaccccga cggactcggc atcgaggtgt acgtcgatcg gccacgcagc
     1321 agttggcgca gggagaacgg ccaggtcgcc atggacaccg cgccgttgga tctcgagagc
     1381 gtcgtgcgcg attccgcggg cgcacggtgg accgggatgc ccaacgggac gcaggtcggc
     1441 cacgtgcatc tgcatgtcgg cgatctggcc atcgcctcgg atttctatca cgccgcgctc
     1501 ggattcgagc gaacggtgtg gagctatccc ggcgcgctct ttctggcggc cgggggctac
     1561 catcaccatc tcgggctcaa cacctgggcc ggcgcggggg cggccaatcc gcgtgacgac
     1621 gacgcgcgcc tcctcgaatg gaccatcgtt ctcccgtcgg ccgagcaggt gcgggagacc
     1681 gccgcgcgcc tggagcggac cgcgcattcg gttgagcgcg atgcgcacgg cgctcgtgcc
     1741 atcgatccgt ggggcacgcg cttccgtatc gccgacgagg agtcgtacgc gcggctcacc
     1801 gcgcgtggcg gcgcatcccg ctgaccacgc gtccatgttg agcctgtcgt cgctcgcggg
     1861 atgggaggag tcggtactct cgagcatcgc gggcgccaac ggcaccgtgg aggagcgcga
     1921 ccggcagatc gagcgctccg gcatgtacgg cgagtatccg gccatcgtgc gcgcgtacat
     1981 cgagctgttc ggcgatgacg attcggcgct cgaggcgctc aagcgcgcgc tctttctggt
     2041 gtggcaaagc gccgtggcgc tcccggcgga taccgggctt gccgcactgc cggagggcac
     2101 cgcgcgactg gtggtggagc agctcgacga cgcgattcgt cgaggcggcg atgacgcgga
     2161 gctgcgctgg atgctcgcgt ggtatcacgc tcacggga
//
LOCUS       DASVII010000699         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_319978,
            whole genome shotgun sequence.
ACCESSION   DASVII010000699 DASVII010000000
VERSION     DASVII010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319978"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1009
                     /locus_tag="VF309_09105"
     CDS             275..1009
                     /locus_tag="VF309_09105"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX7466787.1"
                     /translation="MPFPRIHCDLRLGPGAQFALAPEAAQHVGKALRLKAGDVITVFD
                     GRGGEYEATIQRIDRERVDVKVGSWRDAEVESGLEVGLVHGLPEADKMDWIIQKSVEL
                     GAGWIQPVVCDRSVVRLSAERAAKREAHWRRVAIAACEQSGRNRIPEVRPTLAFQAWL
                     AQRPAIECWMLTPGAAPLAQRPRPAGPFELLIGPEGGLSERERDLALLQGSVPVALGP
                     RVLRTETAPLAALAAINALWGDFSAR"
     gene            1043..>1952
                     /gene="argA"
                     /locus_tag="VF309_09110"
     CDS             1043..>1952
                     /gene="argA"
                     /locus_tag="VF309_09110"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX7466788.1"
                     /translation="MARNPKLKTEAFVKWFRSATPYIHRWGGATFVIAFGGEVLEDGE
                     FQQLTHDINVLVSLEVRVVLVHGTRPQIEKQMRDHGVTPRYVANRRVTDDKALSCVKE
                     ANGIVRVEMEALLSMELANSPMWGADIRVSSGNFVTAKPVGVVNGVDFQHTGEVRKID
                     AEGIRRRLDDDEVVLISPLGFSPTGDVFNCTLEDVATSTAIALGADKLIFLTETAGAL
                     DAKGKLISELTVREAGELIASNNLPEDVQVYLPCAIKACSQGVKRSHLISRHVDGALL
                     IELFTHHGIGTMVVPESPEHIRVATLE"
BASE COUNT          299 a          678 c          670 g          305 t
ORIGIN      
        1 gctgtcactc ggcgtggggc tcctcagcgc cgcgtgcatg agcttctagg cgcgggaaac
       61 cgatatggcc cacgagttca tttccgcgtg ggagcgggcg ggccatcgcg tgctcgttca
      121 tcgcgggctg ggcacgccgc cgcgcgggat cgaggcgttt ctgtagctgc gatggggcgt
      181 ggggcgttcg tgcacgcgtg ctagcatcgg aacgcatcat cccacccatc tcgcgtctcg
      241 cgctccccgc tccccgctcc gcgcccggac ttccatgcct tttccccgca tccactgcga
      301 cttgcgcctc gggcccggcg cccagttcgc gctcgcgccc gaagcggcgc agcacgtggg
      361 caaggcgctg cgattgaagg cgggcgacgt gatcaccgtc ttcgacggcc gcggcggcga
      421 gtacgaggcg accatccagc gcatcgaccg cgagcgcgtc gacgtgaagg tcggctcgtg
      481 gcgcgacgcg gaggtcgagt ccgggctcga agtgggcctg gtgcacggcc tgccggaggc
      541 cgacaagatg gactggatca tccagaagtc cgtcgagctc ggcgcgggat ggatccagcc
      601 ggtcgtctgc gaccgcagcg tcgtgcgcct ttccgcggag cgcgccgcga agcgcgaggc
      661 gcactggcgc cgcgtggcga tcgccgcctg cgagcagtcg gggcgcaacc gcattcccga
      721 ggtgcggccg acgctcgcgt tccaggcgtg gcttgcgcag cgcccggcga tcgaatgctg
      781 gatgctcacg cccggggcgg cgccgctcgc gcagcgcccg cgcccggcag ggcccttcga
      841 gctgctgatc ggtcccgagg gcggactgtc ggagcgcgaa agggacctcg ccctgctgca
      901 gggaagcgtg ccggtcgcgc tcggcccgcg tgtgcttcgc accgagacgg cgccgctggc
      961 cgcgctcgcc gccatcaatg cgctgtgggg agatttctcc gcgcgctaga atcgcgcacc
     1021 agtggatttg cgttgaggaa ccatggccag aaaccccaag ctcaagaccg aagccttcgt
     1081 gaaatggttt cgctccgcca cgccctacat ccatcgctgg ggcggggcca ccttcgtgat
     1141 cgccttcggc ggcgaggtcc tcgaggacgg ggagttccag cagctcacgc acgacatcaa
     1201 cgtgctcgtg agcctcgaag tgcgcgtggt gctggtgcac ggcacgcgcc cgcagatcga
     1261 gaagcagatg cgcgaccacg gcgtcacgcc gcgctacgtg gcgaaccgcc gcgtgaccga
     1321 cgacaaggcg ctctcgtgcg tgaaggaagc caacggcatc gtgcgcgtcg agatggaggc
     1381 gcttctttcc atggagctcg ccaacagccc catgtggggc gccgacatcc gcgtgtcgtc
     1441 cggcaacttc gtgaccgcga agcccgtggg cgtcgtgaac ggcgtcgact tccagcacac
     1501 gggcgaagtg cgcaagatcg acgcggaagg gatccgccgg cgcctcgacg acgacgaggt
     1561 ggtgctcatc tctccgctgg gcttctcccc taccggggac gtcttcaact gcaccctgga
     1621 ggacgtcgcg acctccaccg ccatcgccct gggggccgac aagctcatct tcctcaccga
     1681 gaccgcgggc gcgctcgacg cgaaaggcaa gctcatctcg gagctcacgg tgagggaggc
     1741 cggcgagctc atcgcgtcga acaaccttcc cgaggatgtg caggtctacc ttccctgcgc
     1801 gatcaaggcg tgctcccagg gcgtgaagcg ctcgcatctc atcagccgcc acgtggacgg
     1861 ggcgctcctg atcgaactct tcactcacca cggcatcggc acgatggtag tgcccgagtc
     1921 tcccgagcac atccgcgtcg cgaccctcga gg
//
LOCUS       DASVII010000700         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_428345,
            whole genome shotgun sequence.
ACCESSION   DASVII010000700 DASVII010000000
VERSION     DASVII010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428345"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1216
                     /locus_tag="VF309_09115"
     CDS             128..1216
                     /locus_tag="VF309_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEX7466789.1"
                     /translation="MGAVTVGGGSEARRSGRRVLPIAAATVAILLVVLVGGAYLYDHS
                     RRDVIAHGVSIGDVPVGGLNTTAARQKLERDLVVPLQRPLTVRSGGHAWTLGASEAGV
                     RVDVTNMLDQAVSASRGGSFLSRAFRGVFGGSVKRNIPLVVSYSHAGVRALTVKVRAA
                     INRAPRDATVQPSSSGLGEVRGRVGLKVDSSKLGGRIESALVGDIASRTLTVPTLEVQ
                     PKVTTAQLAQKYPVYLVVDRGAFRLKLYTHLKLAKTYEIAVGMEGLETPPGLHHIEWK
                     QVDPPWYVPKKAWAGKLAGTVVPPGPADPLKARFMSFEGGAGIHGIDPSEYSSIGHDA
                     SHGCVRMRIPDVIDLYGRTPVGTPVYIM"
     gene            complement(1268..1441)
                     /locus_tag="VF309_09120"
     CDS             complement(1268..1441)
                     /locus_tag="VF309_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466790.1"
                     /translation="MIIAMSEALSKDIGLIATFGGIGVVVNIIVVYIAVQVRGERRQN
                     QEYLASRRPPGSS"
BASE COUNT          261 a          484 c          585 g          256 t
ORIGIN      
        1 tcggtgtcaa ccagaattac agctgagact tcgatgtttt ggcgctcgca cgatggcttg
       61 cacgcccgct gtcgtcgcta gactcgaccg ggctggtgtt cgcattcgca aagttcgcga
      121 ggtcctgatg ggcgcagtga cggttggcgg tggctcggag gcgcgccgca gcggaaggcg
      181 cgtgctgccg atcgcggcgg cgacggtggc gatcctgctc gttgtgctcg tcggcggcgc
      241 ctacctgtac gaccactcgc gcagggacgt gatcgcgcac ggcgtgagca tcggcgacgt
      301 accggtgggc ggcctgaaca cgacggcggc gcggcagaag ctcgaacgcg atctcgtggt
      361 ccctttgcaa cggccgctca cggtgcgctc gggcgggcat gcatggactc ttggggcgag
      421 cgaagcgggc gtgcgcgtgg atgtgaccaa catgctcgac caggcggtga gcgctagtcg
      481 cggcggatcg ttcctctcgc gagcgtttcg cggagtgttc ggcggtagcg tcaagcgcaa
      541 catcccgctt gtggtcagct actcccacgc gggcgtgcgt gcgcttacgg tgaaggtgcg
      601 cgcggcgatc aaccgcgcgc cgcgcgacgc gacggtccag ccgagctcga gcggcctggg
      661 agaggtgcgc ggccgggtgg ggctgaaggt ggacagttca aagttgggtg gacgcatcga
      721 gtcggcgctg gtcggcgaca tcgcgagccg tacgctcacg gtgcccacgc tcgaggtgca
      781 gccgaaggtg accacggcgc agttggcgca gaagtatccg gtgtacctcg tggtcgatcg
      841 cggggccttc aggctgaagc tgtacacgca cctgaagctc gcgaagacgt acgaaatcgc
      901 ggtcggcatg gaagggctgg aaacgccgcc gggcctgcac cacatcgaat ggaagcaggt
      961 cgatccccct tggtacgtgc cgaagaaagc gtgggcgggc aagctggcgg gcacagtggt
     1021 gccgccgggg ccggccgacc cgctgaaggc gcgcttcatg tccttcgaag gcggcgcggg
     1081 catccacggg atcgacccga gcgagtactc ctcgatcggc cacgacgcgt cgcacggctg
     1141 cgtgcggatg cgcatcccgg acgtgatcga cctgtacggc cgcacgcctg tgggcacgcc
     1201 cgtctacatc atgtagcgag cgtgaccgcg cgtgcacgac gcgtggtcgc tgcgcgacgg
     1261 ctgaggctca ggaggagccg ggcggacggc gcgaagccag gtattcctgg ttctggcggc
     1321 gctcgccgcg aacctgcacg gcgatgtaca cgacgatgat gttgaccacc acgccgatgc
     1381 cgccgaacgt ggcgatcagg ccgatatcct tgctcagggc ttcgctcatg gcgatgatca
     1441 tgcggggagc ctagcaaccg cggaacgcgc cggtatcgta gccttcgtca tgccgccgtc
     1501 gacgcgagat cgacgcacat gcacccggag aggtgccgga gcggttgaac gggcgcgact
     1561 ggaaatcgcg taacgggggt aacctc
//
LOCUS       DASVII010000701         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_608231,
            whole genome shotgun sequence.
ACCESSION   DASVII010000701 DASVII010000000
VERSION     DASVII010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608231"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="VF309_09125"
     CDS             <1..1749
                     /locus_tag="VF309_09125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7466791.1"
                     /translation="RYYQRPLGEVLGTVLPPRLRQVSRRPLGAPGEAGGAAPRLVEAQ
                     PLTQEQREAIALCDTESDAFQAVLLHGVTGSGKTEVYLHLIARALAAGRQALFLVPEI
                     GLTPQFETQVRQRFPDANLVAAHSHLGEGERAATWLAAQSGAAHIVLGTRLAVLMPFR
                     RLGLIVVDEEHDLSYKQQEGLRYSARDIAVRRAQRLGIPVILGSATPSLETYANARDG
                     RYVLASLTRRASSGSAMPVVRTVDTRADRPHEGFSHALVEAIRGRLAKKEQSLIFVNR
                     RGFSPVLFCRACAWHSTCKRCSANLVVHLGAREMRCHHCGHRSRIPAVCPGCGSADLA
                     PIGHGTQRVEEALVALFPHARIARVDRDSTSRKGSLRGLLEKVRKGEIDILVGTQMLA
                     KGHDYPRLTLVGVLDCDSALFSADFRASERLFSQLVQVSGRAGRGEAPGEVLIQTDFP
                     GHPLFAAVARQDYARFAEDALAERRLAGFPPFSHLALLRAESRKAGEATVFLKLAARL
                     AKRLAARREAKDIEVFDPVPAPLERKAGYERAQLLVRAAPRATLQPFLHEWRLQLADK
                     GGRHIRWSLDVDPQEV"
     gene            1806..>2078
                     /gene="argS"
                     /locus_tag="VF309_09130"
     CDS             1806..>2078
                     /gene="argS"
                     /locus_tag="VF309_09130"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.4"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7466792.1"
                     /translation="MPSPDSKARLAGLVQDAVRKAFPDADVAVELDRPKNAEHGDFAT
                     NVALQLAKRVGRKPREVAEAIVAALGTSGDIAKADIAGPGFINLTVS"
BASE COUNT          307 a          755 c          700 g          316 t
ORIGIN      
        1 cgctactacc agaggccgct tggcgaagtc ctgggaaccg tgctgccgcc acgcttgcgg
       61 caggtgtcgc gccggccgct cggcgctccg ggcgaggcgg gaggagccgc gccgcgactt
      121 gtcgaagctc agccgctcac acaggagcag cgcgaggcga tcgcgttgtg cgacacggaa
      181 tcggatgctt tccaggccgt gctcctccac ggagtcacgg gcagcggcaa gaccgaggtg
      241 tacctgcacc tcatcgcgcg cgccctggcc gcgggacgcc aggcgctctt cctcgtgccc
      301 gagatcgggc tcacgcccca gttcgagact caggtccgcc agcgatttcc cgatgcgaac
      361 ctggtcgccg cccacagcca cctcggcgag ggcgagcgcg cggcgacgtg gctcgcggcg
      421 caatcgggcg ccgcgcacat cgtgctcggg acccgcctcg cggtgctgat gccgtttcgc
      481 cggctcggcc tcatcgtggt cgacgaagag cacgatctct cgtacaagca gcaggaaggg
      541 ctgcgctact cggcacgcga catcgcggtg cggcgcgcgc agcgcctcgg cattcccgtg
      601 atcctcggct cggccacgcc ttcgctcgag acttacgcga acgcgcgcga tggccgatac
      661 gttctcgcat ccctcacgcg gcgcgcatcg agcggctcgg cgatgcccgt cgtgcgcacc
      721 gtcgacacgc gcgccgatcg cccgcacgag ggcttcagcc acgcgctggt ggaagcgatt
      781 cgcgggcgcc tggcgaagaa ggagcaatcg ctcatcttcg tgaaccggcg cggcttctcg
      841 ccggtgctct tctgccgcgc atgcgcgtgg cactcgacct gcaagcgctg cagcgcgaac
      901 ctcgtcgtcc acctcggagc gcgcgagatg cgctgccatc attgcgggca tcgcagccgc
      961 atcccggccg tgtgccccgg ctgcgggtcc gccgatctcg cgcccatcgg ccacggcacg
     1021 cagcgcgtcg aagaagcgct cgtcgcactg tttccccacg ctcgcatcgc tcgagtggat
     1081 cgcgactcga cctcgcgcaa gggttcgttg cgagggctcc tcgagaaggt tcgcaagggc
     1141 gagatcgaca tcctggtggg cacccagatg ctcgccaagg gccacgacta tcccaggctc
     1201 acgctggtcg gcgtgctcga ttgcgacagc gcgctcttca gcgccgactt ccgcgcatcc
     1261 gagcgcctct tctcgcagct cgtgcaggtg tctggacggg cgggacgcgg cgaagcgccc
     1321 ggcgaagtgc tgatccagac ggacttcccc ggccatccgc tgttcgccgc ggtggcgcgc
     1381 caggattacg cgcgcttcgc cgaggacgcc ctcgcggagc ggcgcctcgc gggcttcccg
     1441 cctttctcgc acctggcgct gctgcgcgcc gaatcgagga aggcgggcga ggccacggta
     1501 ttcctaaagc ttgccgcgcg cctcgccaag cgcctcgcgg cgcgccgcga agcgaaggac
     1561 atcgaggtct tcgacccggt cccggcgccc ctcgagcgca aagccggata cgagcgcgcg
     1621 cagctgctcg tgcgcgcggc accgcgcgcg acgctgcaac ccttcctgca cgaatggagg
     1681 ctgcagctcg ccgacaaggg cgggcgccat atccgctggt cgctcgatgt cgatccccag
     1741 gaagtctgag gcggcgcgca cgtgccggcc cgtataatgc ggggtttgga tttcccttcc
     1801 cgctagtgcc ctcccccgat tcgaaagcgc gcctcgccgg cctcgtccag gacgctgtgc
     1861 gcaaggcctt tcccgatgcc gatgtcgcgg tagagctcga ccgtccgaaa aacgccgagc
     1921 acggcgactt cgcgacgaac gtcgcgctgc agctggcgaa gcgcgtcggc aggaagccgc
     1981 gcgaggtggc cgaggcgatc gtggcggcgc tcggcaccag tggcgacatc gcgaaggccg
     2041 acatcgcggg cccgggattc atcaacctca ccgtatcc
//
LOCUS       DASVII010000702         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_428383,
            whole genome shotgun sequence.
ACCESSION   DASVII010000702 DASVII010000000
VERSION     DASVII010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428383"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF309_09135"
     CDS             complement(<1..222)
                     /locus_tag="VF309_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466793.1"
                     /translation="MQTLLNLLAGVALLVWGTHIVRTGVLRIFGADLRRILAKSTSNR
                     GSAFLAGLAVTGVVQSSSATALIASSFVSQ"
     gene            complement(249..1646)
                     /gene="phnY"
                     /locus_tag="VF309_09140"
     CDS             complement(249..1646)
                     /gene="phnY"
                     /locus_tag="VF309_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEX7466794.1"
                     /translation="MNDRARGHAVIEVRNPYTGELAGTVPKASVADVRKAFETARAFR
                     PGLTRYERSEILKKGAELLRAKTEEASDLITRESGLCKKDSLYEVGRVCDVLVFSAME
                     ALRDDGQAFSCDLTPHGKQRRVFTRREPLLGVIVAITPFNHPMNQVAHKVAPAIATNN
                     RVVLKPSEKTPLSAIYLANLLHEAGVPRPMLQVVTGDPREIAGELITHEHADLVTFTG
                     GAAVGKEIAAKAGYRRLVLELGGNDPLIVMEDADLDEASTLAAQGSYKNSGQRCTAVK
                     RILVHQAVARRFVDLLVDKTRAWKYGDPFDRAMDMGTVIDESAAKLFEARVNEAVTGG
                     AKLLVGNRREGALYSPTVVDGVTPAMTLVKEETFGPVSPVIRFGGIDEAIAIANGTAY
                     GLSSAVCTNRLDYITRFVSELHVGTVNVREVPGYRLELTPFGGIKDSGLGYKEGVQEA
                     MKSFTNLKTFSLPWS"
BASE COUNT          262 a          577 c          607 g          309 t
ORIGIN      
        1 ttgcgagacg aaggagctcg cgatgagcgc ggtcgcgctc gaggactgca cgacgccggt
       61 cacggcgagc ccggcgagga acgcggagcc gcggttcgag gtgctcttcg cgaggatgcg
      121 gcgcaggtcg gcgccgaaga tgcgcagcac cccggtcctc acgatgtgag tgccccacac
      181 gaggagcgcg acgccggcga ggaggttgag gagcgtctgc acctgttaga tcgactccat
      241 gcgcaaggtt aactccaggg gaggctgaat gtcttgaggt tggtgaagct cttcatggct
      301 tcctgcacgc cctccttgta gcccaggccc gagtccttga tgccgccgaa gggcgtgagc
      361 tcgaggcgat agccgggcac ctcgcgcacg ttcacggtgc cgacgtggag ctccgagacg
      421 aagcgcgtga tgtagtcgag gcggttggtg cacacggcgg aggacaggcc gtacgcggtg
      481 ccgttcgcga tggcgatcgc ctcgtcgatg ccgccgaagc ggatgacggg cgacaccggg
      541 ccgaaagtct cctccttcac cagcgtcatt gcgggcgtca cgccgtccac caccgtcggc
      601 gagtagagcg cgccctcgcg ccggttgccg acgaggagct tcgcgcctcc ggtgacggct
      661 tcgttcacgc gcgcttcgaa gagcttcgcc gcgctctcgt cgatcacggt tcccatgtcc
      721 atggcgcggt cgaagggatc gccgtatttc cacgcgcgcg tcttgtccac caggagatcg
      781 acgaagcgcc tggcgaccgc ctggtggacg aggatgcgct tcaccgccgt gcagcgctgg
      841 ccgctattct tgtacgagcc ctgcgccgcg agcgtggagg cctcgtcgag gtcggcgtct
      901 tccatcacga tgagcgggtc gttgccgccc agctcgagga cgaggcgccg gtagcccgct
      961 ttcgcggcga tctccttgcc cacggcggcg ccgccggtga aggtgacgag gtccgcgtgc
     1021 tcgtgggtga tcagctcgcc cgcgatctcg cgcgggtctc ccgtcacgac ctgcagcatc
     1081 gggcgcggca cgccggcctc gtggaggagg ttcgcgaggt atatcgcgga aaggggcgtc
     1141 ttctccgagg gcttcagcac cacgcgattg ttggtggcga tcgccggcgc gaccttgtgc
     1201 gccacctggt tcatgggatg gttgaagggc gtgatcgcca cgatcacacc gaggaggggc
     1261 tcgcggcggg tgaagacgcg gcgctgcttg ccgtgcggcg tgaggtcgca cgagaacgcc
     1321 tggccgtcgt ccctcagcgc ctccatcgcg ctgaagacga gcacgtcgca cacgcggccg
     1381 acctcgtaca gggagtcctt cttgcagagg cccgattcac gcgtgatgag atccgaggct
     1441 tcctccgtct tcgcgcgcag gagctccgcg cctttcttga ggatttccga gcgctcgtat
     1501 cgcgtgagtc cagggcggaa ggcccgcgcc gtctcgaacg ccttcctcac gtccgccacc
     1561 gacgccttgg gcacggtccc cgcgagctcc ccggtgtacg gattgcgaac ctcgatcacg
     1621 gcgtggccgc gggcgcgatc gttcatgagg ctgcgacctc ctgcgtctcg tgcctcgtgc
     1681 ctcgcgcctc gagatgattc agcgcgacat cgagaatgtc gaaatttcgc agcttccgtc
     1741 cctcgaggcc cttca
//
LOCUS       DASVII010000703         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_69088, whole
            genome shotgun sequence.
ACCESSION   DASVII010000703 DASVII010000000
VERSION     DASVII010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69088"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF309_09145"
     CDS             <1..533
                     /locus_tag="VF309_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7466795.1"
                     /translation="RSIALGRRAIDEIGHSPENYRLYFPVLERETLISSSKENALDPV
                     LVAALIRQESNFNPLATSPVGARGLMQVMPAVGKAVADTKGIGPWDPDLLYQPAVNIK
                     LGTAHLSGLAHKYPEVVKVLSAYNAGESRVEKWSSKAGAADPEVFTERIPFVETRDYV
                     RTILRNRAYYQALYPW"
     gene            complement(537..725)
                     /locus_tag="VF309_09150"
     CDS             complement(537..725)
                     /locus_tag="VF309_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466796.1"
                     /translation="METHGPLGTLWVSLGLIAFYLALIWLNTLLGVFLTPLFMVPGTW
                     LLDWLKRRKGELKPTSKT"
     gene            763..1734
                     /gene="tilS"
                     /locus_tag="VF309_09155"
     CDS             763..1734
                     /gene="tilS"
                     /locus_tag="VF309_09155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7466797.1"
                     /translation="MTYARVEASEAERAVEAALGTGGPAILAVSGGLDSMVLLTAAAT
                     QLSARARKKIVVATFDHGTGKAAGRAAALVARQAFRSGFACVTGRASTIGTREEEWRR
                     GRWEFLQQVASKREANVVTAHNLDDQVETVFMRILRDAGPRGLAGLYAESEIVRPFLN
                     LSRATLASYARQKNVPFVQDPSNSDRKHLRNRIRLDILPSIVKQNPGFPDELLSLARE
                     AAVWRRSLEDLAGTVGAEAEQDGALRISRSSLKGYDADSLRVLWPALAARASVVMDRR
                     GTQRAAEFTIKGATGGTIQLSGGIEIVMRRDHMLLRRWRSLRPEVLA"
BASE COUNT          344 a          563 c          563 g          315 t
ORIGIN      
        1 cgcgctcgat cgcgctggga cgacgcgcga tcgacgagat cggccacagt cccgagaact
       61 accgattgta tttccccgtt ttagagcgcg agactctgat ctccagcagc aaggagaacg
      121 ccctcgatcc ggttcttgtc gcggcgctca tccggcagga atcgaacttc aatccgctag
      181 cgacttctcc ggtcggagcg agaggcctca tgcaggtgat gccggccgtt ggtaaagccg
      241 tcgcggacac gaaggggatc ggcccgtggg atccggatct tctctatcag cctgccgtca
      301 acatcaagct tggaaccgct cacctgagcg gtctggcgca caagtacccg gaagtggtga
      361 aggttctctc tgcttacaac gcaggggagt cacgggtgga gaaatggtcg agcaaggcgg
      421 gcgcggccga tccggaagtc ttcacggagc gcattccctt cgtcgagacg cgcgactacg
      481 tgcgcacgat cctgcgcaat cgcgcctact accaggccct gtatccctgg tagcgtttac
      541 gtcttggagg tcggtttcaa ctcgcctttt ctgcgcttga gccagtcgag cagccacgtc
      601 cccggaacca tgaacaacgg ggtgagaaac acgccgagca gggtgttgag ccagatgagc
      661 gcgaggtaaa aagctatgag gccgagcgat acccacagcg tgcccagcgg gccgtgcgtt
      721 tccaagcgaa actcccccgt gaataggtag attaaaagta tcatgaccta cgcccgggtt
      781 gaagcatctg aggcagagcg cgctgtcgag gcggcgctgg ggacgggcgg accagctatc
      841 ctcgcggtct cgggcggcct cgattcgatg gtgcttctca ccgcggcggc gacccaactt
      901 tccgcgcgcg cccgcaagaa gatcgtcgtc gccaccttcg atcacggtac gggcaaagcc
      961 gcggggcgcg cggcggcgct ggtagcgcgt caggctttcc gctccggatt cgcttgtgtc
     1021 accggacgag cgtcgaccat cggtacgcga gaggaggagt ggcgtcgcgg ccgctgggag
     1081 ttcctccagc aagtcgcatc gaagcgtgaa gcaaacgtcg tgaccgcaca caacctggac
     1141 gaccaggtcg agaccgtctt catgcgaatc cttcgcgatg ccggcccgcg cggactcgcc
     1201 ggcctctacg cggaatccga aatcgtgcgg ccgtttctaa acctcagccg agccacattg
     1261 gcgagctatg ctcgtcaaaa aaatgtgccc ttcgtccagg atccatcgaa ctccgaccgc
     1321 aagcacctgc gcaaccgcat ccggctcgac attctgcctt cgatcgtgaa gcagaacccc
     1381 ggattcccgg acgagctcct ctcgctggcc cgagaggccg cggtttggcg gcgatcgctc
     1441 gaggatcttg ccggcaccgt cggggccgaa gcagaacagg atggggccct ccgcatcagc
     1501 cgttccagcc tcaagggcta tgacgccgac tcgctccgag ttctctggcc cgccttggcg
     1561 gcacgcgcga gcgtggtgat ggaccgcaga ggaactcaac gcgccgcgga gtttactatt
     1621 aagggagcga cgggagggac gattcagctc tccgggggga ttgagatcgt gatgcggcgg
     1681 gatcacatgc tgctgcgccg ctggcgctcc ttaagaccgg aggtcctggc atgagcagca
     1741 ccatcacgcc gctcgggatc aaagccgatc cacgcctctc cggtc
//
LOCUS       DASVII010000704         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_428450,
            whole genome shotgun sequence.
ACCESSION   DASVII010000704 DASVII010000000
VERSION     DASVII010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428450"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..962)
                     /gene="mmsB"
                     /locus_tag="VF309_09160"
     CDS             complement(78..962)
                     /gene="mmsB"
                     /locus_tag="VF309_09160"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX7466798.1"
                     /translation="MKIGFIGLGHMGNPMARNLLKHGHALKIYDVIPDLVKKLAVEGC
                     EVATSAGECAKGVEVVITMLPSSPHVRSVYLGDYGVLKGAAPGTPLIDSSTIDPLTAR
                     EVAMDAKAKGCAMVDAPVSGGVAGAEAGTLTFMVGGEARDFEGAKPVLLAMGKNIVHC
                     GGIGNGQVAKICNNMMLAIEMIATAEGMTLAAKLGMDPKVFAAIVNTSSGRCWSSDTY
                     NPYPGVLESVPASRSYSGGFGSDLMLKDLTLATDAAKNAKQPVMLGALAQQIYQKHSV
                     DGNGAKDFSSVIMQYMKT"
     gene            complement(959..>2122)
                     /locus_tag="VF309_09165"
     CDS             complement(959..>2122)
                     /locus_tag="VF309_09165"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7466799.1"
                     /translation="FACGIPHLSKGEYTEGAGPSIDIYSMRQPLGVVAGITPFNFPAM
                     IPLWMFPMAIVCGNTFVLKPSEQDPMTPMLLAELALEAGVPPGVLNVVHGGKEAVDAL
                     CTHPDVKAVSFVGSCHVGEHVYKLSSEHGKRAQCMMGAKNHAVVMPDANKEKSLDALV
                     GAGFGAAGQRCMATSVAVLVGEANRWIPDIVAKAKTLKVNVGTEKGTDLGPVISRQAK
                     ERIEGLIAQGVKEGAKLELDGRNTRVAGFESGNFISPTIFSGVKVDHKVYTEEIFGPV
                     LVLVSVDTLDEAIDLVNRNPFGNGTGIFTQSGAAARKFQNEIDVGQVGINVPIPVPVP
                     YFSFTGSRGSKLGDLGPYGKQVIQFYTQVKTVTSRWFDDETTSPGIHTTISLK"
BASE COUNT          328 a          688 c          708 g          398 t
ORIGIN      
        1 gccctcgggc gtccaggtgt tcgccggcgg gttgtcgagc gtgatgaagg cggtgtggcc
       61 gcgcgtctcg tgcttgatca cgtcttcatg tactgcatga tcacgctcga gaaatccttg
      121 gcgccgttgc cgtcgacgga gtgcttctgg tagatctgct gggcgagcgc gccgagcatc
      181 accggctgct tcgcgttctt cgcggcatcg gtggcgaggg tcaggtcctt cagcatgagg
      241 tccgagccga agccgccgga ataggagcgc gaggccggaa cgctctcgag gacgcccggg
      301 taggggttgt aggtgtcgga gctccagcag cggccgctcg aggtgttcac gatcgccgcg
      361 aagaccttgg gatccatgcc gagcttggcc gccagcgtca tgccctcggc ggtggcgatc
      421 atctcgatcg cgagcatcat gttgttgcag atcttggcga cctgcccgtt gccgatgccg
      481 ccgcaatgga cgatgttctt gcccatggcg agaagcaccg gcttcgcgcc ctcgaaatcc
      541 cgtgcttcgc cgcccaccat gaaggtgagc gtgccggctt cggcccccgc gacgccgccg
      601 gagacgggcg cgtcgaccat cgcgcagccc ttggccttcg cgtccatcgc gacctcgcgc
      661 gcggtgaggg gatcgatggt cgacgagtcg atgagcggcg tgcccggcgc ggcgcccttg
      721 aggactccgt agtcgccgag gtagaccgag cgcacgtggg gagaggaggg cagcatcgtg
      781 atgacgacct ccacgccctt ggcgcattcg cccgccgagg tcgcgacctc gcagccctcg
      841 acggcgagct tcttcacgag gtccgggatc acgtcgtaga tcttcagcgc gtggccgtgc
      901 ttgaggagat tgcgggccat gggatttccc atgtgaccca ggccgatgaa tccgatcttc
      961 atttgaggct gatcgtcgtg tggatgccgg gcgacgtcgt ctcgtcgtcg aaccagcgcg
     1021 aggtcacggt cttcacctgc gtgtagaact ggattacctg cttgccgtag gggccgaggt
     1081 cgcccagctt ggagccgcgc gagccggtga agctgaagta gggcaccggc acgggaatcg
     1141 gcacgttgat gccgacctgt cccacgtcga tctcgttctg gaacttgcgc gccgcggcgc
     1201 ccgactgggt gaagattccc gtgccattgc cgaagggatt cctgttcacg aggtcgatcg
     1261 cctcgtcgag cgtgtccacg cttacgagca cgagcaccgg cccgaagatc tcctcggtgt
     1321 agaccttgtg gtccaccttc acgccggaga agatggtcgg cgagatgaag ttgccgctct
     1381 cgaagcccgc gaccctggta ttgcgtccgt cgagctcgag cttcgcgcct tccttcacgc
     1441 cctgagcgat caggccctcg atgcgctcct tcgcctgcct cgagatgacg gggcccaggt
     1501 ccgtgccttt ctcggtgccg acgttcacct tcaaggtctt cgccttcgcg acgatgtccg
     1561 ggatccaccg gttggcctcg cccacgagca cggccaccga ggtcgccatg cagcgctggc
     1621 ccgcggcgcc gaagcccgcg cccacgagcg cgtcgagcga cttctccttg ttggcgtcgg
     1681 gcatcacgac cgcgtggttc ttcgcgccca tcatgcattg ggcccgcttg ccgtgctcgc
     1741 tcgagagctt gtacacgtgc tcgcccacgt ggcacgagcc cacgaaggac accgccttca
     1801 cgtcgggatg ggtgcagagc gcgtcgacgg cctccttgcc cccatgaacg acgttgagca
     1861 cgcccggggg aacgccggcc tctaacgcga gctcggcgag gagcatcggc gtcatcggat
     1921 cctgttccga cggcttgagg acgaatgtgt tgccgcacac gatcgccatc gggaacatcc
     1981 acagcggaat catcgcgggg aagttgaacg gcgtgatacc ggcgacgacg cccagcggct
     2041 ggcgcatcga atagatgtcg atgctcggac cggcgccctc ggtgtattct cccttggaca
     2101 gatgcgggat gccgcaggcg aa
//
LOCUS       DASVII010000705         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_249265,
            whole genome shotgun sequence.
ACCESSION   DASVII010000705 DASVII010000000
VERSION     DASVII010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249265"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..596)
                     /locus_tag="VF309_09170"
     CDS             complement(144..596)
                     /locus_tag="VF309_09170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7466800.1"
                     /translation="MGFVPADPQFEQRTRASFAKQGAMAFLGARLGEVAPGRVEIWLP
                     FRPELAQQHGFFHAGIISTIADTAGGYAGFTLFPADAGVLTVEFKINLMAAADGERAI
                     AVGEVLRSGRTLTVCRLDAFVEKQGRRVHCATGVQTLIALVGRGGVKD"
     gene            complement(624..>1625)
                     /locus_tag="VF309_09175"
     CDS             complement(624..>1625)
                     /locus_tag="VF309_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX7466801.1"
                     /translation="SLPRVRPHFAVKANPDPRVLKVLIQEGAGFEIASIAELDILIGL
                     GVPPAEVFYSNPMKSRAYLEYAAAKGVEWFVVDSVEEIRKVKAVKSDAKMYLRIDAPN
                     IGSDWPLSGKFGAHMSDVPELIETAVQCQADLAGVTFHVGSQCLNPDNWRVGMERARK
                     VFASMRAVGLKPRLLNLGGGYPVRHVKPIPSIETIAEVINKELRHFGPEVQVIAEPGR
                     YLVSDAGYFICRVVGTATRNNQRWMYWDAGVFGGVIETTEGLRYNIYTDRDGEPVSWN
                     VAGPTCDSVDVVMREELFPADLQDGDFIFIKNAGAYTTAYASNFNGFPLPEVATIGS"
BASE COUNT          259 a          568 c          542 g          256 t
ORIGIN      
        1 gctcgtgctc gccggcgtcc ccgggaggct tgagctcgtc gcgatcgagg aagcgcgcga
       61 gccatgcgtc gctcgcgacc aggcccgcct cgccgcgccc gaggagcccg aaaccgctcg
      121 aggcccagaa gtcctgggcc acgtcaatcc ttcacgccgc cgcgcccgac caacgcgatg
      181 agcgtttgca cgccggtagc gcaatgcacc ctgcggccct gcttctcgac gaaggcatcg
      241 agcctgcaca ccgtgagcgt gcggccgctg cgcagcacct cgcccacggc gatggcgcgc
      301 tctccgtcgg ccgcggccat gaggttgatc ttgaattcca cggtgagcac gcccgcatcc
      361 gcgggaaaca gcgtgaaccc cgcgtagccc cccgcggtgt cggcgatcgt cgagatgatt
      421 cccgcgtgga agaagccgtg ctgctgcgcg agctcggggc ggaagggcag ccagatctcg
      481 acccgtcccg gcgccacttc gccgagacgc gcgcccagga acgccatcgc gccctgcttc
      541 gcgaagctcg cgcgcgtgcg ctgctcgaac tgcgggtccg cgggaacgaa gcccatcctg
      601 ttctcgcgcc tggcccgtcg cgcctacgag ccgatcgtcg ccacttcggg gagcggaaag
      661 ccgttgaagt tgctcgcgta cgcggtggta tacgcgccgg cgttcttgat gaagatgaag
      721 tcgccatcct gcaggtccgc cggaaagagc tcctcgcgca tcaccacgtc cacggagtcg
      781 caggtgggac cggccacgtt ccacgagacg ggctcgccgt cgcggtcggt gtagatgttg
      841 tagcgcaggc cctcggtggt ctcgatcacg ccgccgaaga cgcccgcgtc ccagtacatc
      901 cagcgctggt tgttgcgggt cgcggtgccc accacgcggc agatgaagta gcccgcgtcg
      961 gagacgaggt agcggcccgg ctcggcgatc acctgcactt cggggccgaa atggcgcagc
     1021 tccttgttga tcacctccgc gatcgtctcg atggagggaa tcggcttcac gtggcgcacc
     1081 gggtagccgc cgccgagatt gagcaggcgc ggcttcaggc ccacggcgcg catcgaggcg
     1141 aacaccttgc gcgcgcgctc catgcccacg cgccagttgt cgggattgag gcactgggag
     1201 cccacgtgga aggtgacgcc ggcgagatcc gcctggcact gcacggccgt ctcgatgagc
     1261 tcgggcacat cgctcatgtg cgcgccgaat ttccccgaaa gcggccagtc gctgccgatg
     1321 ttgggcgcgt cgatgcgcag gtacatcttc gcatcggatt tgaccgcctt caccttgcgg
     1381 atctcctcga cgctgtccac cacgaaccac tcgacgccct tagccgcggc gtactcgagg
     1441 taggcgcgcg acttcatcgg attggaatag aagacctcgg ccggcggcac gcccagcccg
     1501 atcaggatgt ccagctcggc gatcgaggcg atctcgaagc cggcgccctc ctggatgagg
     1561 accttcagga cgcgcgggtc gggattggcc ttcactgcga agtgcggccg cacgcgcggc
     1621 agcga
//
LOCUS       DASVII010000706         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_572805,
            whole genome shotgun sequence.
ACCESSION   DASVII010000706 DASVII010000000
VERSION     DASVII010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572805"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2449)
                     /gene="pepN"
                     /locus_tag="VF309_09180"
     CDS             complement(<1..2449)
                     /gene="pepN"
                     /locus_tag="VF309_09180"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX7466802.1"
                     /translation="MRDAAPQVNLKDYTPPAFLISTVALDVNIQAARVAVRATLRCTR
                     NAARNSPDEPLVLDGEDLELVSVKIDGGALGASDYRVDARHLTIAKVPASFTLETVVR
                     FDPWKNTKLEGLYATKSGLVTQCEAEGFRRITYFIDRPDVMATYAVTLCADPARFPRL
                     LANGNLVAQGEGEPADWFSREEGARHWARWEDPFPKPCYLFAMVAANLELNEDHFTTQ
                     SGKKALLQIYVEPGKLDQAGFAMQALKKCMRWDEEIFGLELDLERFMIVAVSDFNAGA
                     MENKGLNIFNTKYILARSDTATDGDYFGVDKVVAHEYFHNWTGNRVTCRDWFQLSLKE
                     GLTVYRDQEYGADEYSRPVVRIQEVRELRSRQFPEDAGPMAHPVRPESYVEVNNFYTS
                     TVYEKGAEVVRMYATIVGKEGFRRGMDLYFSRHDGHAVTCDDFRAAMADANDADLSQF
                     GRWYSQAGTPLVECSGDYDGAKETYTLHVKQSCAPTPGQPDKEPLVIPFAVGLVDLDG
                     HDLPIDIDSQSLFTGSRDHGARTAVLALTQAEQSFVFRQVPEHPVLSLLRGFSAPVHV
                     RFAYTEAELTHLMTYDSDAFNRWEAGQALAIRTLLASAESVRAGRAMEVPATFIEAMG
                     RVISDGSKDPAFAAEALQLPDEGYLAECMEVADPEAAHIARLRLMREIATRYRTRFEG
                     AFRHFTAPGPYSPDAAASGRRALRNSALGYVSTIDDGTSRALAFLEFRRAENMTDAMA
                     ALACLANSAGAERERALSMFYEKWKGEALVVDKWFRVQATSWLPGTLDRVKELMGHEA
                     FDLKNPNRARALLHAFAM"
     gene            complement(2559..>3191)
                     /locus_tag="VF309_09185"
     CDS             complement(2559..>3191)
                     /locus_tag="VF309_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7466803.1"
                     /translation="TDIAARGLDIEDLPHVVNFDLPHVPEDYVHRIGRTGRAGQTGEA
                     LSLVGSEDRPLLSAIERLINRKIEVRHVDGFEAGRMSSAQPVEARGEPRERGQGRRPS
                     PQPASQEKRSKPAAQGRGGDGRGKPRGEGRGGEARPPRGGRGESRREERGPRYANAPA
                     ARTSGNAGGMDFNKPYEPSPSASPAAAPDETPGRKRQPQAVPVLFRKKAA"
BASE COUNT          464 a         1057 c         1141 g          529 t
ORIGIN      
        1 ccatcgcgaa ggcgtgcagc agggcgcggg cgcgattggg gttcttgagg tcgaaggcct
       61 cgtggcccat gagttccttc acccgatcga gggttccggg cagccacgac gtcgcctgga
      121 cgcggaacca cttgtcgacg acgagcgctt cgcccttcca cttctcgtag aacatcgaga
      181 gggcgcgctc gcgctccgcg cccgccgaat tcgccaggca cgcgagcgcc gccatcgcgt
      241 cggtcatgtt ctcggcgcgg cggaattcga ggaatgcgag ggcccgcgaa gtcccgtcgt
      301 cgatggtgct gacgtagccg agggccgagt tgcgaagcgc gcgccggccc gaggccgccg
      361 catcgggaga atacgggccc ggcgcggtga aatggcggaa ggcgccttcg aagcgggtgc
      421 gataccgtgt cgcgatctcg cgcatgagcc gcagccgcgc gatgtgggcc gcctccgggt
      481 cggcaacctc catgcactcg gcgagatagc cttcgtcggg aagctgcagc gcctcggcgg
      541 cgaacgcggg gtccttcgat ccgtcggaaa tgacgcggcc catcgcctcg atgaacgtcg
      601 ccggcacctc catcgcgcgg ccggcgcgca cgctttcggc cgacgcgagg agcgtgcgga
      661 tcgcgagcgc ctgccccgcc tcccagcgat tgaacgcgtc ggagtcgtag gtcatcaggt
      721 gcgtgagctc ggcttccgtg tacgcgaagc gcacgtgaac cggcgcggag aagccgcgca
      781 gcagcgaaag caccgggtgc tcgggcacct ggcggaagac gaacgattgc tcggcctgcg
      841 tgagcgcgag gacggcggtg cgcgcgccgt ggtcgcgcga gcccgtgaac agcgattgcg
      901 agtcgatgtc gatcggcagg tcgtgcccgt cgaggtccac gagtccgacc gcgaacggaa
      961 tcacgagcgg ctccttgtcc ggctggcccg gcgtgggcgc gcacgactgc ttcacgtgca
     1021 gcgtgtacgt ttccttcgcg ccgtcgtagt cgccgctgca ctcgaccagc ggcgtgcccg
     1081 cctgcgaata ccagcggccg aattgcgaca ggtccgcgtc gttcgcgtcg gccatcgccg
     1141 cgcggaagtc gtcgcaggtg acggcgtggc cgtcgtggcg gctgaagtag aggtccatgc
     1201 cgcgccgaaa gccttccttg ccgacgatgg tcgcgtacat gcgcaccact tcggcaccct
     1261 tctcgtagac cgtggacgtg tagaagttgt tgacctcgac gtaggactcg ggccgcaccg
     1321 gatgcgccat cgggcccgcg tcctcgggga actggcggct gcgaagctcg cgaacttcct
     1381 ggatccgcac cacggggcgg gagtactcgt cggcgccgta ttcctggtcg cgatagaccg
     1441 tgagcccttc cttcagcgag agctggaacc agtcgcggca ggtcacgcgg ttgccggtcc
     1501 agttgtggaa atactcgtgg gcgaccacct tgtcgacgcc gaagtaatcg ccgtcggtcg
     1561 cggtgtcgga gcgcgcgagg atgtacttgg tgttgaagat gttgaggccc ttgttctcca
     1621 tggccccggc gttgaagtcg gagaccgcga cgatcatgaa gcgctcgagg tcgagctcta
     1681 ggccgaagat ctcctcgtcc cagcgcatgc acttcttcag cgcctgcatc gcgaagcccg
     1741 cctggtcgag cttgcccggc tcgacgtaga tctgcagcag cgccttcttc ccggactgcg
     1801 tcgtgaagtg atcctcgttc agctcgaggt tcgccgccac catcgcgaag aggtagcacg
     1861 gcttcgggaa cgggtcttcc cagcgcgccc agtggcgggc gccttcttcg cgcgagaacc
     1921 agtcggccgg ctcgccctcg ccctgcgcca ccaggtttcc gttcgcgagc aatcgcggga
     1981 accgcgccgg gtccgcgcac agcgtgacgg catacgtcgc catcacgtcg gggcggtcga
     2041 tgaaataggt gatgcggcgg aagccttcgg cctcgcactg ggtgaccagg ccggacttcg
     2101 tcgcgtagag gccctcgagc ttggtgttct tccacgggtc gaagcgcacg acggtctcga
     2161 gcgtgaacga tgcgggaacc ttggcgatcg tgagatgcct tgcatcgacg cggtaatcgc
     2221 tcgcgccgag cgcgccgccg tcgatcttga ccgagacgag ctcgaggtcc tcgccgtcga
     2281 gcaccagcgg ctcgtcgggg gaattgcgcg ccgcgttgcg cgtgcacctg agcgtcgcgc
     2341 ggaccgctac ccgcgcggcc tggatgttca cgtcgagcgc gaccgtcgat atcaggaacg
     2401 cgggcggcgt gtagtccttg aggttcacct ggggagcggc gtcgcgcatg tggaacggac
     2461 gaaggcccgc tgcggatgcg cgggtcccgg aagcggtggg tggaacgcga ttttactcca
     2521 gggagcgcag cgcccccccc gggctcgaac gccgaatatc aggcggcttt cttgcggaaa
     2581 agtacgggga cggcctgcgg ctggcgcttg cgcccgggcg tctcgtcggg cgccgccgcg
     2641 gggctcgccg aaggcgacgg ctcgtagggc ttgttgaagt ccatgccgcc ggcattgccg
     2701 gaggtgcgag ccgcgggcgc attcgcgtaa cgcggtccgc gttcctcgcg ccggctttcg
     2761 ccgcggccac cacggggagg acgcgcctcg ccgccacggc cttcgccgcg cggcttgccg
     2821 cgcccgtcgc cgccacggcc ctgtgcagcg ggcttggagc gcttctcctg cgatgcgggc
     2881 tgcggagaag gcctgcgccc ctgcccgcgc tcgcgcggct cgccacgggc ctccacgggc
     2941 tgcgcggagg acatccgtcc ggcttcgaat ccgtccacgt ggcgcacttc gatcttgcgg
     3001 ttgatgagcc tttcgatggc ggagagcaac ggccggtctt cggagccgac cagcgagagc
     3061 gcctccccgg tctggcccgc gcggccggtg cggccgatgc ggtgcacgta atcctcgggg
     3121 acgtgcggca ggtcgaagtt cacgacgtgg ggtaggtcct cgatgtcgag gccgcgcgcg
     3181 gcgatgtcgg t
//
LOCUS       DASVII010000707         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_608429,
            whole genome shotgun sequence.
ACCESSION   DASVII010000707 DASVII010000000
VERSION     DASVII010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608429"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1593)
                     /locus_tag="VF309_09190"
     CDS             complement(<1..>1593)
                     /locus_tag="VF309_09190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX7466804.1"
                     /translation="RLSAINPARLLATTLDLAARCNFSLNEIRYEYPEELVPAGETPA
                     THLKALTLQGLARRYPQGAPEEVARTVEHELRLIEELAYEPFFLTVNDIVEFARGRGI
                     LCQGRGSAANSAVCYCLGITEVDPGRMSVLFERFISKERNEPPDIDVDFEHQRREEVI
                     QYIYGKYGRERAAIAAVVITYQPRSALRDVGKALGLSLDQVDLLSKSMSWWDNREALK
                     KRLVEAGFDLSNRVVQQVIDLTQQLVGFPRHLSQHPGGFVIDNRRLSRLVPVENASMP
                     DRTVIQWDKDDLDALGLLKVDVLGLGMLSCIRRAIDLVNGYRKSLLTMQSIPAEDPAV
                     YEMIQRADTIGVFQIEARAQMSMLPRLKPEKFYDLVIEVAIVRPGPIMGGMVHPYLRR
                     REGKEKVTYASDEVKKVLERTLGVSIFQEQVMQLAVVAAGFTPGEADKLRRAMASWKR
                     RGGIEPFREKLVQGMLAHGYAREYAEQIYQQIQGFGEYGFPESHAASFALLVYVSSWL
                     KHHEPAAFACALLNSQPLGFYST"
BASE COUNT          270 a          501 c          540 g          282 t
ORIGIN      
        1 gtcgaataga agccgagagg ctggctgttc aggagcgcgc acgcgaaagc ggcgggctcg
       61 tgatgcttga gccagctcga cacgtagacg agaagcgcga agctcgccgc gtgcgattcc
      121 ggaaagccgt attcgccgaa gccctggatc tgctggtaga tctgctcggc gtattcgcgc
      181 gcgtagccgt gcgcgagcat gccttgcacg agcttttccc ggaagggctc gatgccccct
      241 cgcctcttcc acgacgccat cgcgcggcgc agcttgtcgg cctcgcccgg cgtgaagccc
      301 gcggccacca ccgcgagctg catcacctgc tcctggaaga tcgatacgcc aagcgtgcgc
      361 tcgaggactt ttttcacctc gtcgctcgcg taggtgacct tctccttgcc ctcgcgccgg
      421 cgcaggtacg gatgcaccat gccgcccatg atcggccccg ggcgcacgat cgcgacctcg
      481 atcaccaggt cgtagaattt ctcgggcttc aggcggggca gcatcgacat ctgcgcgcgc
      541 gcctcgatct ggaagactcc gatcgtgtcg gcgcgctgga tcatctcgta gaccgcggga
      601 tcttcggcgg ggatggactg catggtgagg agcgacttcc ggtaaccgtt caccaggtcg
      661 atcgcgcgcc ggatgcagga aagcatcccg aggccaagga cgtcgacctt gagcagcccc
      721 agggcgtcga gatcgtcctt gtcccattgg atgaccgtgc gatcgggcat cgacgcgttc
      781 tcgacgggga cgaggcgtga aagccggcgg ttgtcgatca cgaatccgcc gggatgctgc
      841 gagagatggc gcgggaatcc gaccagctgc tgcgtgaggt cgatcacctg ctgcactacg
      901 cggttggaaa gatcgaagcc cgcttccacg aggcgctttt tcagcgcctc gcggttatcc
      961 caccacgaca tcgacttcga gaggagatcg acctggtcga gcgacaaccc cagcgccttg
     1021 ccgacatcgc gcaacgcgct gcggggttga taggtgatca cgaccgccgc gatcgccgcg
     1081 cgctcgcggc catacttgcc gtagatgtac tggatgactt cttcgcggcg ctggtgctcg
     1141 aagtcgacgt cgatgtcggg cggctcgttg cgctccttgg agatgaagcg ctcgaaaagc
     1201 accgacatcc ttccgggatc gacctcggtg atgccgaggc aatagcagac cgcggagttg
     1261 gccgccgagc cgcgcccctg gcagaggatg ccgcggccgc gcgcgaattc cacgatgtcg
     1321 ttcaccgtga ggaagaacgg ctcgtaggcg agctcctcga tgaggcgcag ctcgtgctcg
     1381 acggtccggg cgacttcttc cggagccccc tgcggatagc ggcgcgcgag gccttgcagg
     1441 gtcagtgcct tgagatgcgt ggccggggtt tctccagcgg gcacgagctc ctcgggatac
     1501 tcgtagcgga tctcgttcaa cgagaagttg cagcgggccg cgaggtcgag cgtggtggcg
     1561 aggaggcgcg cggggttgat cgcggagagc cgg
//
LOCUS       DASVII010000708         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_644217,
            whole genome shotgun sequence.
ACCESSION   DASVII010000708 DASVII010000000
VERSION     DASVII010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644217"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /gene="rimM"
                     /locus_tag="VF309_09195"
     CDS             <1..381
                     /gene="rimM"
                     /locus_tag="VF309_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7466805.1"
                     /translation="GWREMALEDFEVHSKGPVAKLAGCDDREGAEKLRGCDVAVRRAQ
                     LREPDDGTMYWVDLVGLEVVDEGGIVLGTVEALFETGETSVMVVRESASGAERMIPFV
                     AQYVKSVDREARRITVEWKADYDA"
     gene            378..1148
                     /gene="trmD"
                     /locus_tag="VF309_09200"
     CDS             378..1148
                     /gene="trmD"
                     /locus_tag="VF309_09200"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7466806.1"
                     /translation="MTHFDVITIFPGMFEAVSRHGITRKALEEKLFEMKTWDPRDFTT
                     DNYRRVDDRPYGGGPGMVMMAEPLQACIEAATSRQRAAGVERPMVVLMSPQGERLDEA
                     LVRGLAAEPGLVLIAGRYEGVDERVVAKSVHREVSIGDYVTSGGELPAMVLIDCLVRR
                     LPGSLNDAESAAQDSFSEGMLDWPHYTRPEKWSEERVPDVLMSGNHAAIARWRRKQAL
                     GRTWDRRPDLVDETKLSREDRQLLEEYRREQQAQQQQQ"
     gene            1236..1604
                     /gene="rplS"
                     /locus_tag="VF309_09205"
                     /pseudo
     CDS             1236..1604
                     /gene="rplS"
                     /locus_tag="VF309_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(1713..>2160)
                     /locus_tag="VF309_09210"
     CDS             complement(1713..>2160)
                     /locus_tag="VF309_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX7466807.1"
                     /translation="PNDRKLYVDLSFYDDLKRRFKAPGDFAQAYVIAHEVGHHVQNLV
                     GTFAKLEAHHDNPTSVRMELQADCYAGVWGHFAAVKNQLDPGDIDEALTAASAIGDDR
                     LQQQSQGRVVPDSFTHGSSAQRVRWFKRGMDSGLVKDCDTFAAGSL"
BASE COUNT          405 a          643 c          798 g          314 t
ORIGIN      
        1 ggatggcgcg agatggcgct cgaggatttc gaggttcact cgaagggccc ggtcgcgaag
       61 ctcgcgggct gcgacgatcg cgaaggcgcc gagaagctgc gcggctgcga tgtcgcggtg
      121 aggcgcgcgc agttgcgcga gccggacgac ggaacgatgt actgggtcga cctggtcggc
      181 ctggaagtgg tcgacgaggg cggaatcgtc ctcggcacgg tcgaggcgct cttcgagacg
      241 ggcgagacga gcgtcatggt ggtgcgcgaa tcggcgtcgg gcgcggagcg gatgattccg
      301 ttcgtcgcgc agtacgtgaa gtcggtggat cgcgaggcgc ggcgcatcac ggtggaatgg
      361 aaggcggatt acgacgcatg acgcatttcg acgtgatcac gatctttccc gggatgttcg
      421 aggcggtgag ccgtcacggc atcacgcgca aggccctcga ggagaagctc ttcgagatga
      481 agacgtggga tccgcgcgat ttcacgacgg acaattaccg cagggtcgac gatcggccct
      541 acgggggcgg gcccggcatg gtgatgatgg ccgagccgct gcaggcgtgc atcgaagcgg
      601 ccacctcgcg gcaacgcgct gcgggcgtgg agcgcccgat ggtggtgctg atgtcgccgc
      661 aaggcgagcg gttggacgag gcgctggtgc gcggcctcgc ggcggagccg ggcctggtgc
      721 tcatcgccgg gcgctatgag ggcgtcgacg agcgcgtggt ggcgaagtcg gtgcatcgcg
      781 aggtctcgat cggcgactac gtcacctcgg gcggcgagct gccggcgatg gtgctgatcg
      841 attgcctggt gaggcgcttg ccgggaagct tgaacgacgc cgaaagcgcc gcgcaggatt
      901 cgttttcgga gggaatgctc gactggccgc actacacgag gccggagaag tggagcgagg
      961 agcgcgtccc cgacgtgctg atgtcgggaa accatgccgc gatcgcgcgg tggcgcagga
     1021 agcaggcgtt gggacgcacg tgggaccgca ggcccgatct ggtcgacgag acgaagctct
     1081 cgcgcgaaga ccggcaattg ctggaggagt accgccgcga gcagcaggcg cagcaacagc
     1141 agcagtagag gaccattaca aaccgcgtgc acccgcctct ttgagtcggc tctgcgcgaa
     1201 aggccagacg gccggaaccc gaaaggaaaa tgaagatgga cctgatccag aagctgcacc
     1261 aggaagaaat caagcggctc ggccgcaaga tcccggagtt cgccccgggc gacacggtga
     1321 tcgtgaaagt caccgtcgtc gaaggcgaca agaagcgcga gcaggcctac gaaggcgtgg
     1381 tgatcgccaa gcgcaaccgc ggcctgaact ccaacttcat cgtccgcaag atgtcgagcg
     1441 gcgagggcgt ggagcgcacg ttccagacgt attcgccggg gatcgcctcc atcgaggtga
     1501 agcggaaggg cgacgtgcgc cgcgccaagc tttattacct gcgcggcctc accggcaaga
     1561 aggcgcgcat ccgcgagaag ctcgaagggc tcgaccgcga cgtgctgcct ccggaagagg
     1621 acgaagccgt cgcagcggtc ggcgcgaagg aagccaagcc cgaaaccaag cccgaagcca
     1681 agcccgaggc caagcccgcc aagagctgag gcctagaggg atccggcggc gaacgtgtcg
     1741 cagtccttca cgagccccga atccatgccg cgcttgaacc agcgcacgcg ctgggccgaa
     1801 gagccgtgcg tgaacgagtc cggcacgacg cgcccctggg attgctgctg aaggcggtcg
     1861 tcgccgatgg cgctcgcggc ggtgagcgcc tcgtcgatgt cgccggggtc gagctggttc
     1921 ttcaccgccg cgaagtgccc ccacacgccc gcgtagcaat ccgcctgcag ctccatgcgc
     1981 accgaggtcg gattgtcgtg gtgcgcctcg agcttcgcga aggtgccgac aaggttctgc
     2041 acatggtggc cgacctcgtg cgcgatcacg taggcctgcg cgaagtcgcc cggcgccttg
     2101 aagcgccgct tgaggtcgtc gtagaacgac aggtccacgt agagcttccg gtcgttcggg
//
LOCUS       DASVII010000709         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_320485,
            whole genome shotgun sequence.
ACCESSION   DASVII010000709 DASVII010000000
VERSION     DASVII010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320485"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VF309_09215"
     CDS             <1..1102
                     /locus_tag="VF309_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7466808.1"
                     /translation="AQSFTRALDKVIELAPEIVLIAGDIFHQVRPTNTAILHAYNQFA
                     RLRRALPNAAIVMVAGNHDTPRSLEAGNILRLFTPLGISVVDDEAQALEFPHLDLSVL
                     GVAHNHRARPKLVPSGTHRWNLLVLHGPVEGEMGHSVHDAAIDEIPQDDLNASQWDYI
                     ALGHYHVYKRFSRNQYYSGALDYTSTNAWGERVEEAQIGVAGKGIIEHDLASGKHIFH
                     PLRPSREFVDLPAISARGLTTEEVDELIRDAVEGCPGGIDDKVVRLVVRNMPRHISRQ
                     LDHKALREYRHRALHFHLDARRPDLIRRQDGQGAPGRRPSLADFVREKLRERVVGADI
                     NRDALVALGLRYLSDAEERESPSAVQPASVDG"
     gene            1103..2079
                     /locus_tag="VF309_09220"
                     /pseudo
     CDS             1103..2079
                     /locus_tag="VF309_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          349 a          666 c          750 g          314 t
ORIGIN      
        1 ggcgcagtcg ttcacgcgtg cgttggacaa ggtgatcgag ctcgcaccgg agatcgtgct
       61 gattgctggc gacatcttcc atcaggtgcg cccgacgaac acggcgattc tgcacgccta
      121 caaccaattc gcgcggctgc ggcgagcgct gcccaacgcg gcgatcgtga tggtggcggg
      181 taatcacgac acgccgcgct cgctcgaggc gggcaacatc ttgcggctct tcacgccgct
      241 gggcatcagc gtggtggacg acgaggcgca ggcgctggag ttcccgcatc tggatctgtc
      301 ggtgctgggt gtcgcgcaca atcatcgggc gcgacccaag ttggtgccgt cagggacaca
      361 tcggtggaac ctgctcgtgc tgcatggtcc cgtggaaggc gagatgggcc attcagtgca
      421 cgatgccgcg atcgacgaga ttccgcagga cgatctcaac gcctcgcagt gggactacat
      481 cgcgctcggc cattaccacg tctacaagcg gttcagcagg aaccagtatt acagtggcgc
      541 gctggactac acgagcacga acgcgtgggg cgagcgcgtc gaggaagccc agatcggcgt
      601 ggcgggcaag gggatcatcg agcatgatct cgccagcggt aagcacatct ttcatccact
      661 ccgtccgtcg cgagagttcg tcgacctgcc ggcgatcagc gcgcgcgggc tgaccaccga
      721 agaggtggac gagctcattc gtgacgcggt cgagggatgt cccggcggca ttgacgacaa
      781 agtcgtgcgg ctggtggtgc gcaacatgcc gcgccacatc tcgcgccagc tcgatcacaa
      841 ggcgctgcgc gagtatcggc accgcgcgct gcactttcat ctcgacgcgc ggcgcccgga
      901 tctcattcgc cgccaggacg ggcagggtgc acccggccgc cggccgtcgc tcgcggactt
      961 cgtgcgcgaa aagctgcgcg agcgcgtggt gggcgccgac atcaatcgcg atgcgctcgt
     1021 cgcgctgggg ctgcgctatc tgagcgatgc cgaggagcgc gagagtccat cggcggtgca
     1081 gccggcgagc gtggacgggt agatgcgcct caacagcctg cacctcgtca actttcgcca
     1141 gcacgccgac agcttcatca acttcgaccc tgggctcacc ggcatcatcg gcccgaacgg
     1201 atagggcaaa tcgacgatcc tcgaggccat cgcgtgggcg ttgtatgggc aggacgcggc
     1261 gcgcggcaag aaggagacga ttcgctggat gcgcgcgggc ccgggcgcga aggtgagcgt
     1321 cgagctcgac ttcgagcttg gcggtcaccg gtatcgcgtc gtgcgcggcc tcacgcaggc
     1381 cgagctcttt ctcgacggcg ccgatgagcc ggtggccaac tcgctcaccg gtgtgacgga
     1441 gatgcttcgt cgccggctcg gcatgagccg cgacgagttc ttcaacacct acttcaccgg
     1501 gcagaaggag ctggacgtga tggcggccat ggcgccgtcg gagcgggcgc agttcctctc
     1561 ccgcgtgctc ggctgcgagc ggctgcgcgc ggcgcagcgg ctgggcaaag acagggcgcg
     1621 cctcatcgcc gccgaagcgt cgggcgtgcg cacggcgatg ccggacgcgc cgaccatcga
     1681 gcgcgtgcat gcggaggcgg agctgcggct cgcgcaggca aacgagcggt ctcgcgtgag
     1741 cgctacgcgg cgcgagcgcg cgagcgcgaa gctgaccgac gtggctccgc gctggctcgc
     1801 cgcgcaacgg gagcgcgagc aacttcagga ggcgctcgcc gagcttcgcg tggccgagag
     1861 cgaggcggcg tcgtatgcac gcgacgcgga gcgcatcgac cgtgagctcg ccgaggtcgc
     1921 gaacgcggag gcggagctcg agaagctcgc ggaggagctc atgccgcttg ccgcgctcag
     1981 cgaggagctg aacgcgctcg accagctctc gcgcgacgag gcgcgccggc aggcgctgct
     2041 cgagacggag cgtgtgctcg acgaggagct gcgccggct
//
LOCUS       DASVII010000710         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_69465, whole
            genome shotgun sequence.
ACCESSION   DASVII010000710 DASVII010000000
VERSION     DASVII010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69465"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /gene="hisH"
                     /locus_tag="VF309_09225"
     CDS             complement(<1..540)
                     /gene="hisH"
                     /locus_tag="VF309_09225"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7466809.1"
                     /translation="MAQRISIVDYGMGNLRSVQKALLHVAPDARVEITAQPEAIRAAD
                     RVVFPGQGAMPDCMRALNESGLGDAVAYAAGSKPFLGLCIGQQMLFERSAEGDTPGLG
                     LLPGRVRAFDRKAGLKIPHMGWNEVWQLVPHPLWKGIADGSRFYFVHSYYCEPTEEAL
                     TAASTSYPAAFTSAIARENI"
     gene            complement(567..1154)
                     /gene="hisB"
                     /locus_tag="VF309_09230"
     CDS             complement(567..1154)
                     /gene="hisB"
                     /locus_tag="VF309_09230"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX7466810.1"
                     /translation="MRKAEIQRDTKETQVRVAVDLDGHGEARLASGIPFLDHMLDQLA
                     RHGGLDLEVKAKGDLDIDAHHTVEDIGITLGMAMAKALGDKKGIRRYGHAYVPLDEAL
                     SRAVVDFSGRPGLEWHVDYRRARVGEFDVDLTHEFFQGFVNHAQATVHIDNLRGDNAH
                     HQCETVFKAFARALRMAVEPDPRAAGIVPSTKGSL"
     gene            complement(1162..>1650)
                     /locus_tag="VF309_09235"
     CDS             complement(1162..>1650)
                     /locus_tag="VF309_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7466811.1"
                     /translation="GGKSLLGEVGRHPNLILVRTVSKLGLAGLRLGLAVGTREWIREF
                     EKLRMPYNVSSLDAAAAELLLGHPEAFARQTTAILAERGRLERDLDGIAGVERFASAA
                     NFILVRLPDGPGAFEALRQRGILVRTFHGSHPLLANCLRLTVGTPDENTRMLEALAAA
                     LG"
BASE COUNT          248 a          548 c          586 g          268 t
ORIGIN      
        1 aatgttctcg cgcgcgatgg ccgaggtaaa agcagccgga tagctcgtcg aggccgcggt
       61 gagcgcctct tcggtgggct cgcagtagta gctgtggacg aagtagaagc ggctgccgtc
      121 ggcgatgccc ttccacaacg ggtgcgggac gagctgccac acctcgttcc accccatgtg
      181 aggaatcttc agccccgcct tgcgatcgaa tgcgcgcacc cggcccggca gcaacccgag
      241 cccgggcgtg tcgccctcgg cgctgcgctc gaagagcatc tgctggccga tgcacaggcc
      301 gaggaagggc ttgctgcccg cggcgtacgc gaccgcgtcg cccaggccgc tttcgttcag
      361 ggcgcgcatg cagtcgggca tcgcgccctg gcccggaaac accacccggt cggcggcgcg
      421 tatggcttcc ggttgcgcgg tgatctccac gcgcgcatcg ggcgcgacgt gcagcagcgc
      481 cttctgcacc gagcgaaggt tgcccatgcc gtaatccacg atcgagatgc gttgagccat
      541 gagggcggtt gaggggctcg aggagcctac aagcttccct tggtcgacgg gacgattccc
      601 gccgcgcgcg gatccggctc gacggccatg cgcagcgcgc gcgcgaaggc cttgaacacc
      661 gtctcgcact ggtggtgcgc gttgtcgccg cgcaggttgt cgatgtgcac cgtcgcctgc
      721 gcgtggttca cgaagccctg gaagaattcg tgcgtgaggt cgacgtcgaa ttcgcccacg
      781 cgggcgcggc ggtaatcgac gtgccactcg agcccggggc gcccggaaaa atcgaccacg
      841 gcgcgcgaca gcgcctcgtc gagcggcacg tacgcgtgcc catagcggcg gatgcccttc
      901 ttgtcgccga gcgccttcgc catcgccatg ccgagcgtga tgccgatgtc ctcgacggta
      961 tggtgcgcgt cgatgtcgag gtctcccttg gccttcacct cgaggtcgag cccgccgtgg
     1021 cgcgcgagct ggtcgagcat gtggtcgagg aaggggattc ccgaggcaag gcgcgcctcg
     1081 ccatggccgt cgaggtcgac ggcgacccgg acctgggttt ccttggtgtc tcgctggatt
     1141 tcggccttgc gcattggggg gtcagccgag ggcggcggcg agtgcctcga gcattcgcgt
     1201 gttctcgtcg ggcgttccga ccgtgagcct caggcagttg gcgagcaggg gatgcgagcc
     1261 gtggaatgtg cgaaccaata taccacgctg ccgcaaggcc tcgaaggcgc ccggcccgtc
     1321 gggcaggcgc acgaggatga agttcgccgc ggaggcgaag cgctcgactc ccgcgatccc
     1381 gtcgaggtcg cgctcgaggc gcccgcgctc ggcgaggatc gcggtggtct gccgcgcgaa
     1441 cgcctcggga tggcccagga gaagctccgc cgccgccgcg tcgagggagc tcacgttata
     1501 gggcatgcgc agcttctcga attcgcggat ccattcgcgc gttccaacgg cgaggcccag
     1561 gcgcagcccg gcgaggccga gcttggagac ggtgcgcacc aggatcaggt tcggatgcct
     1621 gcccacctcg cccaggagcg acttccctcc
//
LOCUS       DASVII010000711         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_249521,
            whole genome shotgun sequence.
ACCESSION   DASVII010000711 DASVII010000000
VERSION     DASVII010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249521"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF309_09240"
     CDS             <1..422
                     /locus_tag="VF309_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX7466812.1"
                     /translation="EVTLAALFRQVEIPLAESRLAEQPVFFGGPVQFDHGFVLHRPLG
                     AWKSTLPVGEIGLTTSRDILEAMASGGGPSEQLVALGYAGWAPGQLEDEIGRNGWLTV
                     QADLDLIFNVAPEDRYQAAMSALGVNAANLSEEAGHA"
     gene            491..913
                     /gene="ruvX"
                     /locus_tag="VF309_09245"
     CDS             491..913
                     /gene="ruvX"
                     /locus_tag="VF309_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7466813.1"
                     /translation="MIGFDFGAKRIGFAVGETSTRIANPLGAIEAEANDARLLEIDRI
                     VAEWHPVAFVVGEPHHADGSEHQVARLAGKFARRLAARYGIPVVMVDETLTSATAEAQ
                     LRDTRTRASRKSDVDALAATIILQSYLDEPEGRSHVAA"
     gene            900..1394
                     /gene="pyrR"
                     /locus_tag="VF309_09250"
     CDS             900..1394
                     /gene="pyrR"
                     /locus_tag="VF309_09250"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX7466814.1"
                     /translation="MSLPDPEALVRSMADAMRATIAPDAALVGIYTGGAWVADRLHRA
                     LALPTPLGLLAVTLHRDDFGRIGLHRESRRSQIPFPVDGREVILVDDVLHTGRTIRAA
                     LNELFDFGRPRAVRLAILADRGGRELPMCPDFLGAKVEVGRDEDLVLSNDNGKLRFAA
                     VKRP"
     gene            1400..>1629
                     /gene="pyrB"
                     /locus_tag="VF309_09255"
     CDS             1400..>1629
                     /gene="pyrB"
                     /locus_tag="VF309_09255"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646758.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7466815.1"
                     /translation="MSRSSVQIGADGRLKHLLTTEGLPAEMIRHILDTADQFVSVGER
                     EVKKVPLLRGKTIFNVFFEGSTRTRTTFEIAA"
BASE COUNT          242 a          572 c          576 g          239 t
ORIGIN      
        1 tcgaggtgac gctcgcggcg ctcttccgcc aggtggagat cccgctcgcc gagtcgcggc
       61 tcgccgagca gccggtgttc ttcggcggcc cggtgcagtt cgatcatggc ttcgtgctgc
      121 atcgcccgct gggcgcctgg aaatcgacgc tgcccgtcgg ggagatcggc ctcaccacct
      181 cgcgcgacat cctcgaggcg atggcctccg ggggcgggcc cagcgagcag ctggtcgccc
      241 tgggctatgc gggctgggcg ccgggccagc tcgaggacga gatcggccgc aacggctggc
      301 ttaccgtgca ggcggatctc gacctcatct tcaacgtggc ccccgaggac cgctaccagg
      361 ccgcgatgag cgccctcggc gtgaacgccg cgaacctctc cgaggaggcg gggcacgcgt
      421 gatgtcaggc tccggcgccc gcgccccgca ggagggtcga ggccccggca ggcaagccgt
      481 ttcggggacc gtgatcggat tcgatttcgg ggcgaaacgc atcggcttcg ccgtgggcga
      541 gacctcgacg cgcatcgcga atccgctggg agcgatcgag gccgaggcca acgacgcgcg
      601 cctgctcgag atcgatcgca tcgtcgccga atggcatccc gtcgcgttcg tggtcggcga
      661 gccgcatcat gccgacggat ccgagcacca ggtggccagg ctcgcgggca agttcgcgcg
      721 gcggctcgcg gcgcgctacg gaatcccggt cgtgatggtc gacgagacgc tcacctccgc
      781 gaccgccgaa gcgcagcttc gcgacacgcg cacgcgcgcc tcgcgcaagt ccgacgtcga
      841 cgcgctcgcg gcgacgatca tcctgcaaag ctatctcgac gagcccgagg gaagatccca
      901 tgtcgctgcc tgaccccgag gccctggtgc ggagcatggc cgacgcgatg cgcgccacga
      961 tcgcgcccga cgccgctctc gtcggcatct acaccggggg cgcgtgggtc gccgatcggc
     1021 tgcatcgcgc gctcgcgctg ccgacgccgc tggggctcct cgcggtgacg ctgcatcgcg
     1081 acgacttcgg gcgcatcggc ctgcaccggg aatcgcgccg cagccagatt cccttcccgg
     1141 tggacgggcg cgaggtgatc ctggtcgacg acgtgctgca tacgggccgc accatccgcg
     1201 cggcgctgaa cgagctcttc gatttcggaa ggccgcgggc ggtgcgcctc gcgatcctcg
     1261 ccgaccgcgg cgggcgcgag ctgccgatgt gccccgactt cctcggcgcg aaggtggagg
     1321 tcggccgcga cgaggacctc gtcctgtcga acgacaacgg caagctgcgt ttcgccgcgg
     1381 tgaagcgacc gtgagcccga tgtcgcgctc gagcgtccag atcggcgccg acgggcgcct
     1441 gaagcatctg ctcaccaccg aaggattgcc ggccgagatg atccgccaca tcctcgacac
     1501 cgcggaccag ttcgtgtcgg tgggcgagcg cgaggtgaag aaggtgccgc tgctgcgagg
     1561 caagacgatc ttcaacgtgt tcttcgaggg ctcgacgcgc acgcgcacca cgttcgagat
     1621 cgccgcgaa
//
LOCUS       DASVII010000712         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_716724,
            whole genome shotgun sequence.
ACCESSION   DASVII010000712 DASVII010000000
VERSION     DASVII010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716724"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VF309_09260"
     CDS             <1..733
                     /locus_tag="VF309_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX7466816.1"
                     /translation="GVPSPLANRKPGDIDFWVVRENTEGEYSSVGGRMFPDTDREFVT
                     QQTVMTRIGVDRILKFAFELAQSRPKKHLTSATKSNGISITMPYWDERVEAMAKNYPK
                     VKWDKYHIDILTANFVLHPDWFDVVVGSNLFGDILSDLGPACTGTIGIAPSGNINPPG
                     LFPSVFEPVHGSAPDIAGQGIANPIGMIWSGAMMLEHLGEKTAAQSIVAAIERTLGER
                     TLRTRDLGGNADTTACGKAVAEMVE"
     gene            complement(959..>1718)
                     /locus_tag="VF309_09265"
     CDS             complement(959..>1718)
                     /locus_tag="VF309_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7466817.1"
                     /translation="TALPTAIAKQCKGLKHVVFLGTGARSYMNPEELAELGIEVHLIK
                     GYGDTAVAECAIALMWEAARGLSRMDRGVRAGNWLRDDAMQLTGKTLGLIGFGGIASE
                     VARIALGSGMRVVAWNRSPKKHPKVEFVELDELLTESDVVSIHLLLNDETRGLVSRAC
                     IEAMKPGVILVNTARGAIVDEEAMIDALNSGQIRHAGLDVFNTEPLPADHPLTKIPNV
                     TLSAHSAFRTPEASENLIAAAWAHCRRIAKGKKA"
BASE COUNT          302 a          556 c          539 g          321 t
ORIGIN      
        1 cggggtgccg tcccccttgg ccaaccgcaa gcccggcgac atcgatttct gggtggtgcg
       61 cgaaaacacc gagggcgaat attcctccgt cggcgggcgg atgttcccgg ataccgaccg
      121 tgaattcgtc acccagcaga ccgtgatgac gcgcatcggc gtcgaccgca tcctgaagtt
      181 tgccttcgag ctcgcgcagt cacggccaaa gaagcatctg acctcggcga ccaagtccaa
      241 cggcatctcg atcacgatgc cgtactggga cgagcgcgtg gaggcgatgg cgaagaatta
      301 tccgaaagtg aagtgggaca agtatcacat cgacatcctg accgcgaatt tcgtgctgca
      361 tccggactgg ttcgacgtcg tggtgggctc gaacctgttc ggcgacatcc tgtccgacct
      421 cggccccgcc tgcaccggca ccatcggcat cgcgccgtcg ggcaatatca atccgcccgg
      481 actgtttccc tcggtgttcg aaccggtgca cggttccgcg cctgatatcg ccggacaagg
      541 catcgccaat ccgatcggca tgatctggtc gggcgcgatg atgctggaac atctcggcga
      601 gaagaccgcg gcccagtcga tcgtcgccgc catcgagcgc acgctgggcg aacgcacgct
      661 gcggacacgg gatctcggcg gcaacgcgga tacgactgcg tgcggcaagg ccgtcgcgga
      721 gatggtggag taattttctt ccttctcccc ttgtgggaga aggtggcgcg aagcgccgga
      781 tgaggggtct ctttccacgg agacagaccc ctcatccgcc tacgctgcgc tccggcacct
      841 tctcccacaa ggagagaagg gaagtaagag cgtagggtgg gcaaagtata gcgtgcccac
      901 cattcacgtt gacagtctgg agtggtgggc acggcgcgaa gtcgcgcctt tgcccaccct
      961 acgctttctt ccccttcgca atccgtcggc agtgcgccca cgcagccgca atcaaattct
     1021 cgctcgcttc tggcgtgcgg aacgccgaat gcgctgacag cgtgacgttg ggtattttcg
     1081 tcaggggatg atccgccggc agcggctcgg tgttgaagac gtcgagcccg gcatggcgga
     1141 tctggcccga gttcaacgcg tcgatcatcg cttcctcgtc gacgatcgcg ccccgcgcgg
     1201 tgttgaccag gatgacgccg ggcttcatcg cctcgataca ggcgcgcgac accagcccgc
     1261 gggtttcgtc gttgagcaag agatggatcg agaccacgtc gctttcggtc agcaactcgt
     1321 cgagctcgac gaattcgacc ttcggatgct tcttcggcga ccggttccag gcgacgacgc
     1381 gcatgccgct gccgagcgca atgcgcgcca cttcggaggc gatgccgccg aaaccgatca
     1441 ggcccagcgt cttgccggtt agctgcatgg cgtcgtcgcg cagccagttg ccggcgcgta
     1501 ctccccggtc catgcgacta aggccgcgcg cggcttccca catcagcgcg atcgcgcatt
     1561 cggcgaccgc ggtgtcgcca tagcccttga tcaggtgcac ctcgatgccg agctcggcta
     1621 gctcctccgg gttcatgtag ctgcgcgcgc cggtgccgag gaacaccacg tgcttcagtc
     1681 ccttgcactg tttcgcaatc gcggtcggta gcgcggtg
//
LOCUS       DASVII010000713         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_285164,
            whole genome shotgun sequence.
ACCESSION   DASVII010000713 DASVII010000000
VERSION     DASVII010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285164"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..500)
                     /locus_tag="VF309_09270"
     CDS             complement(141..500)
                     /locus_tag="VF309_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModE family transcriptional regulator"
                     /protein_id="HEX7466818.1"
                     /translation="MRHARGPAKLRLMFGPEIAIGPGKADLLDAIAATGSISESARRM
                     GMSYRRAWLLVDTMNRCFREPVVAAATGGSGGGGAALTPFGRRALAQFRAMQARVDRA
                     LKGDLDRFSDLLADRPR"
     gene            complement(525..848)
                     /gene="grxD"
                     /locus_tag="VF309_09275"
     CDS             complement(525..848)
                     /gene="grxD"
                     /locus_tag="VF309_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX7466819.1"
                     /translation="MSVQDKIREQVTTNPVVLYMKGSPQFPQCGFSATATQLLKMSGA
                     DDLFTVDVLQNPDIREGIKQFANWPTIPQLYVNGEFVGGCDIMREMYQSGELQKVIAA
                     AKKPG"
     gene            complement(871..1683)
                     /gene="prmC"
                     /locus_tag="VF309_09280"
     CDS             complement(871..1683)
                     /gene="prmC"
                     /locus_tag="VF309_09280"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7466820.1"
                     /translation="MLTLKAALEEATAAIDRVDAHVLMAHVLGVNRAYLAANPMRILS
                     ETEGARVDMLVARRAMGQPVAYLVEKREFYGRDFRVSPDVLIPRPETEVLVEAVLSRL
                     GPGAACVDLGTGSGAIAVTLACERRDARVTATDSSEAALDVARGNAATHACDIEFLRG
                     SWYGPLAGRRFDLIVANPPYVAAGDAHLRDGDLRFEPAQALTDASRDGLDSIRAIVAG
                     ASDHLAAGGWLLVEHGYDQGLAVESLLAAAGFRETASIADLAGIPRVAGGKI"
     gene            complement(1691..>1808)
                     /gene="prfA"
                     /locus_tag="VF309_09285"
     CDS             complement(1691..>1808)
                     /gene="prfA"
                     /locus_tag="VF309_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167486.1"
                     /note="recognizes the termination signals UAG and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF1; this protein is
                     similar to release factor 2; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7466821.1"
                     /translation="NLTLHKIDSIMDGDLAELTGALAAEHQAELLAGLAQAS"
BASE COUNT          256 a          587 c          689 g          276 t
ORIGIN      
        1 gcgaggatga atatggccag gacgcgacgg gcgggttcca tgaggggctt ccgggcgatc
       61 ggaccccgta atataccgcc ggatacaacg cctgcgtcat cgagcctttc ccggtccctt
      121 cgtgggcggg caggggaagc ctagcgggga cggtcggcga ggaggtccga gaacctgtcg
      181 agatcgccct tgagggcgcg gtcgacgcgg gcctgcatgg cgcggaattg cgcgagggcg
      241 cggcgcccga agggcgtgag cgccgcgccg ccgccgcccg agccgccggt ggccgcggcg
      301 accaccggct cgcggaagca gcggttcatc gtgtccacga ggagccaggc gcgccgatac
      361 gacatgccca tgcgccgcgc ggactcggag atggatccgg tagcggcgat ggcgtcgaga
      421 aggtcggcct tgcccgggcc gatcgcgatc tccggcccga acatgagccg cagcttcgcg
      481 gggccgcgcg cgtggcgcat ggacgctcgc gcgggcaacg cgcctcagcc gggcttcttc
      541 gcggcggcga tgactttctg cagctcgccc gactggtaca tctcgcgcat gatgtcgcag
      601 cctccgacga actcgccgtt cacgtagagc tgggggatcg tcggccagtt ggcgaactgc
      661 ttgatgccct cgcggatgtc ggggttctgc agcacgtcga cggtgaagag gtcgtccgcg
      721 ccgctcatct tgagaagctg ggtcgcggtg gccgaaaagc cgcactgcgg aaactggggc
      781 gagcccttca tgtagagcac caccgggttg gtcgtgacct gctcgcggat cttgtcctgc
      841 acgctcatgg ttatctcctt ggaactcacg tcatatctta ccgccggcga cgcgcgggat
      901 gccggccagg tcggcaatgg aagcggtttc gcgaaagccc gccgccgcga ggagcgactc
      961 caccgcgagg ccctggtcgt agccgtgctc gacgaggagc cagccgccgg cggcgaggtg
     1021 atccgatgcg cccgcgacga tcgcgcggat cgagtcgagc ccgtcgcggc tcgcgtcggt
     1081 gagggcttgc gcgggctcga agcgcaggtc gccgtcgcgc aggtgcgcgt cgccggcggc
     1141 gacgtagggg gggttcgcga cgatgaggtc gaagcggcgg ccggcgaggg gcccgtacca
     1201 cgagccgcgc aggaattcga tgtcgcatgc gtgcgtggcg gcgttgcctc gcgcgacgtc
     1261 gagggccgct tcgctcgagt cggtggcggt gaccctggcg tcgcggcgct cgcaggcgag
     1321 cgtgacggcg atcgccccgc ttccggtccc gaggtcgacg cacgcggcgc cgggcccgag
     1381 gcgggagagg acggcttcga ctaggacctc ggtttcgggg cgcgggatga gcacgtcggg
     1441 gctcacgcgg aagtcgcggc cgtagaactc gcgcttctcg acgaggtagg cgacgggttg
     1501 ccccatggcg cgccgggcca cgagcatgtc gacgcgcgcg ccctcggtct cgctgaggat
     1561 gcgcatcggg ttcgcggcga ggtaggcccg attcacgccg aggacatggg ccatgagcac
     1621 gtgggcatcg acgcggtcga tcgccgccgt cgcttcctcg agcgccgcct tcagtgtcag
     1681 catcgcgggc tcagctcgcc tgcgcgagcc ccgcgaggag ctccgcctgg tgctcggccg
     1741 cgagcgctcc ggtgagctcg gcgaggtcgc catccatgat cgagtcgatc ttgtgcagcg
     1801 tgagattg
//
LOCUS       DASVII010000714         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_104581,
            whole genome shotgun sequence.
ACCESSION   DASVII010000714 DASVII010000000
VERSION     DASVII010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104581"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /gene="lysA"
                     /locus_tag="VF309_09290"
     CDS             <1..1016
                     /gene="lysA"
                     /locus_tag="VF309_09290"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7466822.1"
                     /translation="GELARVLAAGGRADRVIFSGVGKSRGEMRAALEAGIRCFNVESE
                     PELERLDAIAGALGKRAPVSLRINPDVDARTHPYISTGLAGNKFGIPHDRALAAYRKA
                     ASLPNLRVIGIGCHIGSQILDTAPLDEALDRVLELAQSLAAQGMKPEHLDLGGGFGIR
                     YRDEVAPDIRGYCEALAKKLSSAGYQVMLEPGRAIVGPAGMLLTRVEYLKLDKAKRFA
                     VVDASMSELMRPALYGSYHEIVEVSERGAGAPQRFDVVGPVCESTDVLGTDRALAIEP
                     GSLLAILSAGAYGMAMASNYNTRVRPAEVLVDGARALEVRPRETIESLFSAEKRIDDA
                     PAR"
     gene            complement(1212..1532)
                     /locus_tag="VF309_09295"
     CDS             complement(1212..1532)
                     /locus_tag="VF309_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466823.1"
                     /translation="MINGVMTGVTAGAIGFIWAIELIAGLRLAAFFLAGFFFAALRAG
                     FFFPAFLEDFFFEAFFLEDFFFEDFFFATRFLEDFFFAFLAFLVDFFFAAFFFAAMVY
                     SFQC"
     gene            complement(1673..2185)
                     /gene="thpR"
                     /locus_tag="VF309_09300"
     CDS             complement(1673..2185)
                     /gene="thpR"
                     /locus_tag="VF309_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX7466824.1"
                     /translation="MRLFFALWPGERVRASLAHVAGTLASFADGKPVSGAKIHLTLAF
                     LGEIGSERLVAVRDAAMQARAPRFELVLDEAGTFRRAGVAWAGPSHVPEALADLQSTL
                     GLALRARGFVLEDRPFAAHVTLARRISTGVPRAPMPAIRWRPRDFALVRSETGTGRYT
                     VMETWPLGRG"
     gene            complement(2188..>2348)
                     /locus_tag="VF309_09305"
     CDS             complement(2188..>2348)
                     /locus_tag="VF309_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX7466825.1"
                     /translation="NLVVAAIAGIFVPLAMERFGRDPAVGSSVFLTFITDSMGFFIFL
                     GLATVFLL"
BASE COUNT          479 a          786 c          757 g          326 t
ORIGIN      
        1 gcggagagct cgcgcgggtg ctcgccgcgg gaggccgcgc cgatcgcgtg atcttctcgg
       61 gcgtgggcaa gtcgcgcggc gagatgcgcg ccgcgctcga ggccggcatc cgctgcttca
      121 acgtggagag cgagcccgag ctcgagcgcc tcgacgcgat cgccggggcg ctgggcaagc
      181 gcgcgccggt gtcgctgcgc atcaaccccg acgtcgacgc gcgcacccat ccctatatca
      241 gcaccggcct cgccgggaac aagttcggca ttccgcacga tcgggcgctc gccgcgtatc
      301 gcaaggcggc gtcactgccg aacctgcggg tgatcggcat cggctgccac atcggatcgc
      361 agatcctcga caccgccccc ctcgacgaag cgctcgatcg cgtcctcgag ctcgcccaat
      421 ccctcgccgc ccagggcatg aaaccggagc acctggatct cggcggaggc tttggcatcc
      481 gttatcgcga cgaggtggcg ccggacatcc gcggctactg cgaggctctc gccaagaagc
      541 tctcgtccgc gggctatcag gtgatgctcg agccgggccg cgccatcgtc ggtcccgcgg
      601 ggatgctcct cacgcgcgtc gagtacctga agctcgacaa ggcgaagcgc ttcgcggtcg
      661 tcgacgcgtc catgagcgag ctcatgcgcc ccgcgctcta cggctcgtac cacgagatcg
      721 tcgaagtgag cgagcgcggc gcgggagcgc cgcagcgctt cgacgtggtg gggccggttt
      781 gcgagtcgac cgacgtcctc ggcaccgatc gcgcactggc gatcgagccg ggcagcctcc
      841 tcgcgatcct ttcggccggc gcatacggca tggcgatggc gtcgaactac aacacccgcg
      901 tgcggcccgc cgaagtgctc gtcgacggcg cccgcgcgct cgaagtccgt ccgcgcgaga
      961 cgatcgagtc cctcttctcg gccgagaagc gcatcgacga cgccccggcg cgatgatttg
     1021 ttcgatcgac gccgcattgg tatcggctcg ctaccaattt ggatcgagtc ggatcaaata
     1081 gtcactcttg acatgcgcgg tttgtgcggc attgcgcata gaatcgcctc accaacttgt
     1141 tcgatgcgaa tccgaacgct tggtagtgcg gcaaaccacc catccgggag gaccgcacga
     1201 cagacaatcg gtcaacattg aaaggaatac accatggcag cgaagaaaaa agccgcgaag
     1261 aaaaaatcca ccaaaaaagc caaaaaagcg aagaagaaat cttctaagaa acgcgtagcg
     1321 aagaagaaat cttcaaagaa gaaatcttcc aagaagaagg cttcgaagaa gaaatcttcc
     1381 aagaaggctg ggaagaagaa gccggcccgc aaggcggcaa agaagaagcc agccaagaag
     1441 aaagcggcca ggcgtaagcc tgcgatgagt tcgatggccc agatgaagcc gatggctccc
     1501 gcggtcaccc cagtcatcac gccgttgatc aagccgatga gctaagagag ttccgcaagg
     1561 actcgaaagc aagccggtga gacccgaata cgaggcgcga cccgaccggt cagccgcgca
     1621 cgcggccagc agcacggccg ggtagcgcct cgcaagcggg cgagttgccc tcctagcccc
     1681 gtcccagggg ccacgtttcc atcaccgtgt aacgccccgt tcccgtttcc gatcgcacca
     1741 gcgcgaagtc ccgtggccgc caccggatcg ccggcatcgg cgctcgcggc acgccggtcg
     1801 agatcctgcg cgcgagcgtc acgtgcgccg cgaacggccg gtcttccaat acgaatcccc
     1861 gggcacgcag cgccaagccg agggtcgact ggaggtcggc gagcgcttcg ggcacgtggc
     1921 tcggccctgc ccatgcgacc ccggccctgc gaaaggtgcc cgcctcgtcg agaacaagct
     1981 cgaagcgcgg cgcgcgcgcc tgcatcgcag catcgcggac cgccaccagc cgctccgacc
     2041 cgatctcgcc gaggaaggcg agcgtgaggt ggatcttggc gccggaaacg ggcttgccgt
     2101 ccgcgaatga cgcgagcgtg ccggccacgt gcgcgagcga tgcgcgcacg cgctctccgg
     2161 gccagagcgc gaagaacagc cgcatggcta caagaggaaa accgtggcca ggccgagaaa
     2221 gatgaagaat cccatgctgt cggtgatgaa cgtgaggaaa acgctcgagc ccaccgccgg
     2281 gtcgcggccg aaacgctcca tcgcgagcgg cacgaagatg ccggcgatcg ccgcgaccac
     2341 gaggttca
//
LOCUS       DASVII010000715         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_320617,
            whole genome shotgun sequence.
ACCESSION   DASVII010000715 DASVII010000000
VERSION     DASVII010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320617"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1827)
                     /locus_tag="VF309_09310"
     CDS             complement(<1..>1827)
                     /locus_tag="VF309_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7466826.1"
                     /translation="IYLTDVADVVDGAENAKLAAWMNDTPAIIVNVQRQPGANVIEVV
                     DRVKLLLPTLTAALPASVDVQLLTDRTVTIRASVHDTQFELMLAVALVVMVIFVFLRS
                     ATATLIPAAAVPLSIVGTFGVMYLAGFSINNLTLMALTIATGFVVDDAIVVIENIARY
                     VEEGEDPMQAAFKGSRQIAFTIISLTFSLIAVLIPLLFMGEVVGRLFREFAITLAVSI
                     LISAFVSLTLTPMMAARLLKHTPPEKQGKFFRKTGEMFDRVIARYGEGLDWVLDHQPL
                     TMLVFVCTLALTVILYIAIPKGFFPLQDTGLIQGISEGPQTASFAAMSGRSRLLAQAI
                     LEDPAVESVSSFIGVDGTNITLNAGRLLIALKPHKARDSVSVVMARIKKRLADLEGMT
                     LYMQPVQDLTIEDRVSRTQYQFTLESANAEELATWTRNVVEKLRMLPQVMDVASDLQD
                     QGLQAYVTINRDTAGRLGVTPAAIDNALYNAFGQRLISTIFTQATQYRVVLEVKPEFR
                     LGPKSLDDIYVPGSGATQAAGSSASASGSVSNSAPSSGGTQVPLSAVATVHEQPGALS
                     IGHLGQFPASTISFNLAPKASLGEAVDKIRAAERDIGLPVSVQ"
BASE COUNT          299 a          579 c          645 g          304 t
ORIGIN      
        1 ctgcacgctc acgggcagtc cgatgtcccg ctccgcggcg cggattttgt ccaccgcttc
       61 gccaagggag gccttgggcg ccaggttgaa ggagatggtg gacgccggaa actggccgag
      121 gtggccgatc gacagcgccc cgggctgctc gtgcacggtc gcgacggcgc tcaacggcac
      181 ctgcgtcccg ccgctgctcg gcgcgctgtt gctgaccgac ccgctggcgg acgcgctgga
      241 gccggccgcc tgcgtcgcgc cgctacccgg cacatagatg tcgtcgagcg atttgggtcc
      301 caggcggaac tccggcttca cctcgagcac cacccgatac tgggtcgcct gcgtgaagat
      361 ggtcgagatg agccgctggc cgaacgcgtt gtagagcgcg ttgtcgatcg cggccggcgt
      421 cacgcccagt cgtcccgcgg tgtcgcggtt gatcgtcacg taggcctgca ggccctggtc
      481 ctgcaagtcg gaggcgacgt ccatcacctg cggcagcatg cgcagcttct cgacgacatt
      541 gcgggtccac gtggccagct cctccgcgtt agcgctctcg agcgtgaact ggtactgcgt
      601 gcggctcacg cgatcctcga tggtgaggtc ctgcaccggc tgcatgtaga gcgtcatccc
      661 ctcgaggtcg gcgaggcgct tcttgatccg ggccatcacg acgctcacgg agtcgcgcgc
      721 cttgtggggc ttcagcgcga tgaggagcct gcccgcgttg agcgtgatgt tggtgccgtc
      781 gaccccgatg aacgaggaga cgctctccac ggcgggatcc tcgaggatcg cctgcgcgag
      841 caggcggctg cggccggaca tcgccgcgaa ggacgccgtc tgcgggcctt cggagatgcc
      901 ctggatgagg ccggtgtcct gcagcgggaa gaaccccttc gggatcgcga tgtagagaat
      961 cacggtgagc gcgagcgtgc agacgaacac gagcatcgtg aggggctggt ggtcgagcac
     1021 ccagtcgagc ccttcgccat acctggcgat gacgcggtcg aacatctcgc ccgtcttgcg
     1081 gaagaacttt ccctgcttct cgggcggcgt gtgcttgagc aggcgcgccg ccatcatcgg
     1141 cgtgagcgtg agcgacacga acgcggagat gaggatcgac accgcgagcg tgatcgcgaa
     1201 ctcgcggaag agccttccca cgacctcgcc catgaagagg agcgggatga ggaccgcgat
     1261 gagggagaac gtgagcgaga tgatggtgaa ggcgatctgc cgcgatccct tgaaggcggc
     1321 ctgcatcggg tcctcgccct cctcgacgta gcgcgcgatg ttctcgatca cgacgatcgc
     1381 atcgtccacc acgaagcccg tcgcgatggt gagcgccatg agggtgaggt tgttgatgct
     1441 gaagcccgcg aggtacatga cgccgaacgt gccgacgatg gacaggggca ccgcggccgc
     1501 ggggatgagc gtcgccgtgg cgctcctcag gaagacgaag atcaccatca ccacgagggc
     1561 gacggcgagc atcagctcga actgcgtgtc gtgcaccgag gcgcggatgg tgaccgtgcg
     1621 gtcggtgagc agctgcacgt cgacggacgc cggcagcgcg gcggtgagcg tgggcaggag
     1681 cagcttcacg cgatcgacga cctcgatcac gttcgcgccc ggctggcgct gcacgttcac
     1741 gatgatcgcc ggcgtgtcgt tcatccatgc cgcgagcttc gcgttctcgg cgccgtcgac
     1801 cacgtcggcg acgtcggtaa ggtatat
//
LOCUS       DASVII010000716         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_608738,
            whole genome shotgun sequence.
ACCESSION   DASVII010000716 DASVII010000000
VERSION     DASVII010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608738"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1591
                     /locus_tag="VF309_09315"
     CDS             <1..>1591
                     /locus_tag="VF309_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466827.1"
                     /translation="THWDREWYHPVGRFRQRLVALIDALLDDREDIAPFLLDGQAIVL
                     DDYLEVRPERREELRARLRDGSLEAGPWYVLADELIPSGESLVRNLLAGRAVLDALGA
                     TAPRVLYSPDAFGHPAMLPAIAAGFGMLLIVLWRGYGGAQHPRGDVARWIAPDGSSAL
                     LYHLSPSGYELGSSLPLDDASARERWSELRATLAPRATVAHVLLPNGADHHARQAGRA
                     RAVTALAAAARPDDVRLTSLGDFAQAIILAAANAPDIQSVSGELRDSYGYAWTLQGTF
                     ATRSALKRRAARAERSLLRNTEPWVALAERVGGVNRDPLMRSAWKALLQCQPHDTLCG
                     CSVDAVARAMAARLEDVEIQSAGLREDALFDLIGYDAIATRSAPASWKSSVVVCNPAA
                     RTRAGVAEVEVVLARDHVRVGPGSGGDAAMPIGAQLPNWMLGAGDVPVQRLTGTRRHE
                     RLESPQHYPWNDFVESTRALVWVSHIDGYGTRAYALNTGDASTIARIPDEVRTEGLSM
                     QNGAIRVESDGRGIVHIATMDG"
BASE COUNT          223 a          579 c          546 g          243 t
ORIGIN      
        1 acgcattggg atcgcgagtg gtatcacccg gtgggccgct ttcgccagcg actcgtcgcc
       61 ctcatcgacg cgctgctcga tgatcgcgag gacatcgcgc cctttctcct cgacgggcag
      121 gcgatcgtgc tagacgacta cctagaagtg cgacccgagc gacgcgagga gttgcgcgcc
      181 cgattgcgcg acggatcact cgaggcgggg ccgtggtacg tgctcgccga tgagctgatc
      241 ccctcgggcg aatcgctcgt gcgcaatctc ctcgccggtc gcgccgtgct cgacgcgctc
      301 ggcgccacgg ctccgcgcgt gctctactct cccgacgcat tcgggcatcc ggcgatgctc
      361 cccgcgattg ccgccggctt cgggatgcta ctcatcgtac tctggcgcgg ctacggcggc
      421 gcacagcatc cgcgcggcga cgtcgcccgc tggatcgcgc cggacgggag ctcggcgctt
      481 ctctaccatc tctcgccgag cggctacgag ctcggctcat cgctgccgct cgacgatgcg
      541 tccgcgcgcg agcgatggag cgagctgcga gcgacgcttg ctccgcgcgc gaccgtcgcg
      601 cacgtgctcc ttcccaacgg cgccgatcat cacgcgcggc aggccggtcg cgcacgagcc
      661 gtgaccgcgc tcgcggccgc cgcgcgcccc gacgacgtcc ggctcacgag cctcggcgac
      721 ttcgcgcagg cgatcatcct cgccgctgcg aacgcccccg acatacaatc ggtaagcggc
      781 gagcttcgcg attcgtacgg ctacgcgtgg acgctgcagg gcaccttcgc cacgcggagc
      841 gccctcaagc gccgcgcggc gcgcgccgag cgctcgctgc tccgcaacac cgagccgtgg
      901 gtggcgctcg ccgagcgtgt gggcggcgtc aatcgcgacc cgctgatgcg ctcggcgtgg
      961 aaagcgctcc tgcaatgcca gcctcacgac acgctctgcg gatgctccgt cgacgccgtc
     1021 gcgcgcgcga tggccgcgcg gctcgaggat gtggagattc aatccgcggg gctgcgcgaa
     1081 gatgcgctct tcgatctcat cggctacgac gccatcgcca cgcgctccgc gcccgcgtca
     1141 tggaagtcat cggtggtcgt gtgcaatccc gccgcgcgca cgagagccgg tgtggccgag
     1201 gtggaagtgg tgctcgcgcg cgatcacgtg cgcgtggggc ccggctcggg cggcgacgcc
     1261 gcgatgccga tcggcgcgca gctgcccaac tggatgctcg gcgcgggcga cgtcccggtg
     1321 caacgactca ccggcacgcg ccggcacgag cggctcgagt cgccgcaaca ctatccgtgg
     1381 aacgacttcg tcgagtcgac gcgcgcgttg gtgtgggtgt cgcacatcga tggctacggc
     1441 acgcgcgcct acgcgctgaa caccggcgac gcgtcgacca ttgcgcgaat tcccgacgag
     1501 gtgcgcaccg aggggctctc gatgcagaat ggcgcgatcc gcgtcgagtc cgacgggcgc
     1561 ggcatcgtcc acatcgccac gatggacggc t
//
LOCUS       DASVII010000717         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_393333,
            whole genome shotgun sequence.
ACCESSION   DASVII010000717 DASVII010000000
VERSION     DASVII010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393333"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2380)
                     /locus_tag="VF309_09320"
     CDS             complement(<1..2380)
                     /locus_tag="VF309_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7466828.1"
                     /translation="MQLIDMQDYSPAARKYWWTVVFLGVWALIYAISRVGQLEGAQLL
                     QVLMGALVAAIVGFFPVQIPGSKTCIAGGEIFIFLVLLVYGAPAAVLAAAVEGLVGAW
                     RSSKRWTSRIITPAMASLAMLTCGSLFDFARDLIAKAGLSSATLLVAAMLLSVLYWIA
                     NIFLTSALVALKRGAPIEPKRWLRDMGWIGLAYTTGASIASVLYFSFEYFGLPAVLIA
                     VPLIVMFISSLHYYFEHKESGERHVEELKESESRFHSAFTHAAIGMALVSTDGKFIQA
                     NKAFCEMLGRNTSELLATTLVKLVNIEDVEALQSPVEKLVRGELSSVHTEVRGLHRDG
                     SDVWMSLNISLARDWQFRTHNLIVQAQDVSARRRAEAELYHNAYHDNLTQLSNRVHFD
                     EQLNRAIARVQRHPEQRFAVMYLDFDRFKMVNDSLGHKAGDELLVNVAQRLKTMLRDT
                     DVLARLGGDEFAILVEDLDRQRDAVDLAERIHKELQKPILLGTMEVAISASIGITFST
                     NNYQTSDQIVRDADIAMYKAKSKGKAQYALFDSSLHAHVSAQLTLENELRKALGGGQI
                     YLDYQPICTLRDQKLVGFEALARWNHPERGLLEPSMFIPVAEETGLIVPLGNWVLSEA
                     CRQMRDWRAIRDSSVLRMSVNVSSLQLTHPDFIAHVARSLQAGEMRPEQLTLEVTESV
                     LMNGIENAISTLMALRQMGVQLSIDDFGTGYSSLSYLATLPIDALKVDRSFIDKMTRE
                     GEGSEIVRAIFKLGQALSKEVYAEGIETSAQLALLQELGCEFGQGFLLSRPVN"
BASE COUNT          408 a          752 c          843 g          410 t
ORIGIN      
        1 cgttcaccgg acgcgacagg aggaagccct gcccgaactc gcagccgagc tcctggagga
       61 gcgcgagctg cgcgctggtc tcgatcccct cggcgtagac ctccttggaa agcgcctggc
      121 cgagcttgaa gatcgcgcgg acgatctcgc tgccctcgcc ctcgcgggtc atcttgtcga
      181 tgaacgagcg gtcgaccttg agcgcgtcga tcggcagcgt ggcgaggtac gagagcgacg
      241 agtaaccggt gccgaagtcg tcgatcgaca gctgcacgcc catctggcgc agcgccatca
      301 gcgtggagat cgcgttctcg atgccgttca tgaggacgct ctcggtcacc tcgagcgtga
      361 gctgctcggg gcgcatctcg ccggcctgca gcgagcgggc gacgtgcgcg atgaagtcgg
      421 gatgcgtcag ctgcaggctg gagacgttca cgctcatgcg cagcacggag ctgtcgcgga
      481 tcgcgcgcca gtcgcgcatc tggcggcacg cttccgaaag gacccagttg ccgagcggca
      541 cgatgagacc ggtctcctcg gcgacgggga tgaacatcga cggctcgaga aggccgcgct
      601 cgggatgatt ccagcgcgcg agcgcctcga agcccacgag cttctggtcg cgcagggtgc
      661 agatcggctg gtagtcgagg tagatctggc cgccgccgag ggccttgcga agctcgttct
      721 cgagcgtcag ctgcgcggag acgtgcgcgt gcaggctcga gtcgaacagc gcgtactgcg
      781 ccttgccctt ggacttcgcc ttgtacatcg cgatgtcggc gtcgcgcacg atctggtcgg
      841 aggtctggta gttgttggtg ctgaaagtga tgccgatgct cgcgctgatc gcgacttcca
      901 tcgtccccag gaggatcggc ttctgcagct ccttgtggat gcgctcggcg aggtcgaccg
      961 cgtcgcgctg ccggtcgagg tcctccacga ggatcgcgaa ctcgtcgccg cccagccgcg
     1021 cgaggacgtc ggtgtcgcgc agcatcgtct tcaggcgctg ggccacgttc accaggagct
     1081 cgtcgcccgc cttgtggccg agcgagtcgt tcaccatctt gaagcggtcg aaatcgaggt
     1141 acatcaccgc gaaccgctgc tccggatggc gctgcacgcg cgcgatggcg cggttcagct
     1201 gctcgtcgaa atgcacgcgg ttggagagct gcgtgaggtt gtcgtggtag gcgttgtggt
     1261 agagctccgc ctcggcgcgg cgccgggcgc tcacgtcctg ggcctggacg atcaggttgt
     1321 gggtgcggaa ctgccagtcg cgcgcgaggg agatgttgag cgacatccag acgtcggagc
     1381 cgtcgcgatg caggccgcgg acctcggtat gcacgctcga cagctcgccc ctgacgagct
     1441 tctcgaccgg cgactggagc gcctcgacgt cctcgatgtt caccagcttc acgagggtcg
     1501 tggcgaggag ctccgaggtg ttccttccga gcatctcgca gaacgccttg ttcgcctgga
     1561 tgaacttccc gtcggtggag accagggcca tgccgatcgc ggcgtgcgtg aaggcgctgt
     1621 ggaagcggct ctcgctctcc ttcagctctt ccacgtggcg ttcgcccgac tccttgtgtt
     1681 cgaagtagta gtgcagcgac gagatgaaca tcacgatgag cggcaccgcg atgaggaccg
     1741 ccggaaggcc gaaatactcg aagctgaagt agagaacgga agcgatcgat gcgccggtcg
     1801 tataggcgag accgatccat cccatgtccc gcagccagcg cttcggctcg atcggggcgc
     1861 cgcgcttcag ggcgacgagg gccgacgtga ggaagatgtt ggcgatccag tagagcaccg
     1921 agagcagcat cgccgcgacc aggagcgtgg cgctgctgag gcccgccttc gcgatgaggt
     1981 cgcgcgcgaa gtcgaagagg gagccgcacg tgagcatcgc gagcgaggcc atcgccggag
     2041 tgatgatgcg gctcgtccag cgcttggagc tgcgccacgc cccgaccagc ccttcgacgg
     2101 cggcggcaag caccgcggcg ggcgcgccgt acacgaggag cacgaggaag atgaagatct
     2161 cgccgccggc gatgcacgtc ttcgagccgg ggatttgcac cgggaagaat cccacgatgg
     2221 cggcaaccag cgcgcccatc aggacctgga gcagctgagc gccctcgagc tggccgaccc
     2281 gggagatggc gtatatcagc gcccagaccc cgaggaacac caccgtccac caatacttgc
     2341 gagcggcggg gctgtagtcc tgcatgtcga tgagttgcat gagacggcct ctatcgttgt
     2401 tggcgttagc ggt
//
LOCUS       DASVII010000718         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_644563,
            whole genome shotgun sequence.
ACCESSION   DASVII010000718 DASVII010000000
VERSION     DASVII010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644563"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..901
                     /locus_tag="VF309_09325"
     CDS             308..901
                     /locus_tag="VF309_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEX7466829.1"
                     /translation="MDHAAHLKKHFEEGTELRQRMAEILPAQIARAGEALAIALKSGK
                     KVLSCGNGGSAADSQHFAAEMVGRFERERRGVPGIALTTDTSALTAIANDYGFDRVFS
                     RQVEALGREGDFLLAISTSGNSKNVIEAIRAAQALDLVVIALTGRDGGAMGKMLLPRD
                     FHLNVAHPRTMRVQEIHLLAIHCLCEVVDNVLFGEKK"
     gene            898..1473
                     /locus_tag="VF309_09330"
     CDS             898..1473
                     /locus_tag="VF309_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7466830.1"
                     /translation="MTRKILGLIVLASAIPVLQGCFPLAVTGVGAAAVMANDRRTTGT
                     YLEDETIEWKVIGRVRERFSGAHVNATSFNRRVLLTGEAPAEEAKQQIEEAVRTIDNV
                     RDVTNELQVAGASSLASRGSDVLVSSNVKARMVNNKKFSPLHVKVVTEAGVVYLMGLV
                     SPQEGDAAVEVARTTSGVSRVVKVFEYVGNK"
     gene            1662..>2714
                     /locus_tag="VF309_09335"
     CDS             1662..>2714
                     /locus_tag="VF309_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX7466831.1"
                     /translation="MALTATLALSAVGWIGLDRSRSDDARSQFDRRTATAEAAIRSRM
                     LSYEQVLRAGAARIASTPSVSRREYRDFIAQLGLEERFPGIQSIGFAERVKASQRDAH
                     VKRVRAEGFPDYEIRPTGERDEMFPTVFNEPALARNARFVGFDAYSDTIRRAAMDRAR
                     DNADAAITGKLVLIGDAYRGTQPQQPGFVMYIAVFDKDVRTLGPAERDAALRGFVFSP
                     FRVHELMRGILDEGVLQVLDMQVYDGPAMSPANELIDTRTAWRVTAADSVPTFSRQVS
                     FPMPGRLWTIRFISRPEFDAALQHTRPWGVLGAGILASVVLFFLTVALVAAWNRAHHL
                     SMRDPLTGLYNRRYLEE"
BASE COUNT          456 a          924 c          899 g          435 t
ORIGIN      
        1 aggccgcggc gcggcaatac ctcgcgcgcc ttcgccgcga gcccgcctgc cgcttcgacg
       61 tcgtcactct cgacggcggc acgcccgtct ggctcaaggg ggctttcgaa gcgcgtgaag
      121 tagaatcgtc cagttatgcg agctgagatt ccttagaatc cgcagataaa cgcagatgaa
      181 cgcggcaacc ccaagggcaa gttcaatggc cccgatgtgc cggggttctt tcacacggaa
      241 aggttctact gcgttcatct gcgtttatct gcggatcgag ccttagtccg cgcgggcggg
      301 aagtgaaatg gaccacgcag cccatctgaa gaagcatttc gaagaaggca ccgagcttcg
      361 ccagcgcatg gccgagattc ttccggcgca gatcgcgcgc gcgggcgaag ccctcgctat
      421 cgcgctcaag tccgggaaga aggtcctgtc gtgcggcaac gggggctcgg ccgccgacag
      481 ccagcatttc gccgcggaga tggtgggacg attcgagcgc gagcgccgcg gcgtgccggg
      541 catcgcgctc accacggata cctcggcgct caccgccatc gccaacgact acggcttcga
      601 ccgcgtgttc tcccggcagg tcgaggccct cgggcgggaa ggcgatttcc tcctcgccat
      661 ctcgacctcg ggaaactcga agaacgtgat cgaggcgatc cgggccgcgc aggcgctcga
      721 cctcgtcgtc atcgcgctca ccgggcgcga cggcggcgcc atggggaaga tgctccttcc
      781 ccgcgacttc cacctcaacg tcgcccatcc gcgcaccatg cgggtccagg agatccacct
      841 cctcgcgatc cactgcctgt gcgaggtcgt cgacaacgtg ctattcggag agaagaaatg
      901 acgcgcaaga ttctcggcct catcgtgctg gcatcggcga ttcccgtctt gcaggggtgc
      961 tttccgctcg cggtcaccgg cgtcggcgcc gcggcggtga tggcgaacga tcgccgcacc
     1021 accggaacct acctcgagga cgaaaccatc gaatggaagg tgatcggcag ggtgcgcgag
     1081 cgcttctccg gcgcgcacgt gaacgccacc agcttcaacc gccgcgtgct cctcacgggt
     1141 gaggcgcccg ccgaggaggc gaagcaacag atcgaggagg cggtgcggac catcgacaac
     1201 gtgagggacg tcaccaacga gctgcaggtc gccggcgcct cctcgctcgc ttcgcgcggc
     1261 agcgacgtgc tcgtctcgtc gaacgtgaag gcgcgcatgg tgaacaacaa gaagttctcg
     1321 ccgctgcacg tgaaagtcgt gaccgaggcg ggcgtcgtgt atctcatggg cctcgtctcg
     1381 ccgcaggaag gcgacgcggc ggtcgaggtc gcgcgaacca cgagcggcgt gagccgcgtg
     1441 gtgaaggtat tcgaatacgt cggcaacaag tgaccgttca tcggcccgcg gcgcgcgttc
     1501 gtgcgcgcgg gcgattacag ctgtattcgc aggagtgacg caccgcactt cgacgcgcaa
     1561 cccccccaag gacaatccat tgcacaaaga tccattgagg acgtgcgatg cgttggggat
     1621 tcgaaggagc aggcgaagcg ctgcgttcac ccataccgtg ggtggcgctc accgcgacgc
     1681 tggcgttgtc cgcggtgggc tggatcgggc tcgatcgcag ccgctccgac gacgcgcgct
     1741 cgcagttcga caggcgcacc gcgaccgcgg aggccgcgat ccgctcgcgc atgctctcct
     1801 acgagcaggt ccttcgcgcc ggcgcggcgc gcatcgcctc gaccccgtcg gtctcgcgcc
     1861 gcgagtaccg cgatttcatc gcgcagctgg gactcgagga gcgctttccg ggcatccagt
     1921 cgatcgggtt tgccgagcgc gtgaaggcct cgcagcgcga cgcgcacgtg aagcgcgtgc
     1981 gcgccgaggg ctttcccgac tacgagatcc gcccgaccgg tgagcgcgac gagatgttcc
     2041 cgaccgtatt caacgagccg gccctggcgc gcaacgcgcg cttcgtgggc ttcgacgcgt
     2101 attccgatac gatccgccgc gcggcgatgg atcgcgcccg ggacaacgcc gacgccgcga
     2161 tcacgggcaa gctcgtcctg atcggcgatg cctatcgcgg aacgcagccg cagcaacccg
     2221 gcttcgtcat gtacatcgcc gtgttcgaca aggacgtgcg cacgctgggc ccggccgagc
     2281 gcgacgccgc gctgcgcgga ttcgtcttca gcccgtttcg cgtgcacgag ctcatgcgcg
     2341 gcatcctcga cgaaggcgtg ctgcaggtgc tcgacatgca ggtctacgac gggcccgcca
     2401 tgagccccgc gaacgagctc atcgacacgc gtaccgcgtg gcgcgtgacg gccgccgact
     2461 cggtgcccac gttctcgcgc caggtgagct tcccgatgcc ggggcgcctc tggacgatcc
     2521 ggttcatctc gcggccggag ttcgacgcgg cgctgcagca cacgaggccc tggggcgtgc
     2581 tgggcgccgg aatcctcgcg agcgtggtcc tcttcttcct cacggtggcg ctggtcgccg
     2641 cgtggaaccg cgcgcaccac ctgtcgatgc gcgatccgct caccggcctc tacaaccgcc
     2701 gctacctcga ggaa
//
LOCUS       DASVII010000719         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_34126, whole
            genome shotgun sequence.
ACCESSION   DASVII010000719 DASVII010000000
VERSION     DASVII010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34126"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..640)
                     /locus_tag="VF309_09340"
     CDS             complement(59..640)
                     /locus_tag="VF309_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7466832.1"
                     /translation="MHPALRVISFEHRSISSVLSGLSHFAERGVEGKKLPNAAVFRAM
                     LHYLDLFAERMHHPKEDAYLFARLRQRTHMADDVLDRLQEDHFYGAGALREVEHAFLR
                     YEEGGEPFFADFAREVSRYVAFHREHMRVEETFVFPLAERELTEDDWREIEAAFAANH
                     DPLASAEQVHDLESLFTRIVTITPAPIGVGDEV"
     gene            740..952
                     /locus_tag="VF309_09345"
     CDS             740..952
                     /locus_tag="VF309_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466833.1"
                     /translation="MSHGKEKAMFESFVIGPVAGNRLGVVRQCIDCVTAAGPGARPCP
                     GFGPGCLSTPVAEVSPARPKPGGESR"
     gene            973..1311
                     /locus_tag="VF309_09350"
     CDS             973..1311
                     /locus_tag="VF309_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466834.1"
                     /translation="MNALATLALALAAAASTPLVAADGGACRPGAAMSGMRERIVSLQ
                     EQMDRIEWTADRAEQRRLMDLHAKRMREGMREIRNRELEPACRMEMMTSMMDVMIRHQ
                     QAMEDPEERR"
     gene            1312..>1930
                     /locus_tag="VF309_09355"
     CDS             1312..>1930
                     /locus_tag="VF309_09355"
                     /inference="COORDINATES: protein motif:HMM:NF023578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HEX7466835.1"
                     /translation="MVEAALDPRVEAEARDCFHCGLPVPEGVRFGFRAAEEWRAFCCA
                     GCEAVARVIAGEGLDDYYRLRSAAPPRLERSEAPDLGLYDHDAMQARFVREDANGIRE
                     AELVVEGIRCAACAWLIEKAVARVPGVESLAVNATTRNARLRWNPALGTLSSVFGAIH
                     RVGYRAWPRETAALELIERRERNSLLRRLWVAGLGMMQVMMYAVPA"
BASE COUNT          289 a          637 c          701 g          303 t
ORIGIN      
        1 ttcagctcgg tttccgtggt gacgaacgcg ctgcgcctgc gcaaggcctg agggccggct
       61 agacctcgtc gcccaccccg atgggagccg gggtgatggt cacgatgcgg gtgaagagcg
      121 attccaggtc gtgcacctgc tcggccgacg cgagcggatc atggttcgcc gcgaacgcgg
      181 cctcgatctc gcgccagtcg tcctcggtga gctcgcgctc cgccaggggg aagacgaacg
      241 tctcctcgac gcgcatgtgc tcgcggtgga aggccacgta gcggcttact tcgcgcgcga
      301 agtccgcgaa gaacggctct ccgccttcct cgtagcgcag gaacgcgtgc tccacttcgc
      361 gcagcgcccc ggccccgtag aagtgatctt cctgaagccg gtcgaggacg tcgtctgcca
      421 tgtgcgtgcg ctggcgcagg cgggcgaaga ggtacgcgtc ctccttgggg tggtgcatgc
      481 gctcggcgaa aaggtcgagg tagtgcagca tcgcgcggaa caccgccgcg ttgggcagct
      541 tcttgccttc caccccgcgc tcggcgaaat gcgacagccc cgaaagcacg ctcgagatgg
      601 agcggtgctc gaaagagatg acgcgaagcg cgggatgcat ggcgggtctc cgggttgcgt
      661 tttgtcgagg ctacccggcg tccatcgcct cgggttgatt ccggtcaagg ggcgagcgca
      721 cgcgcgacgt agcctgtgga tgtcccacgg caaggagaag gcaatgttcg agtccttcgt
      781 gatcggcccc gtggcgggga accgcctggg cgtcgtgcgc caatgcatcg attgcgtcac
      841 cgcggccggt cccggcgcgc gaccctgccc cggtttcggc cccggctgtc tgtcgacgcc
      901 cgtcgccgag gtctctcccg cgcgccccaa gcccggcggc gaatcccggt gaagacttcc
      961 gggagaatcg cgatgaatgc tctcgcgacg cttgcgctcg cattggcggc cgccgcgtcg
     1021 acgccgctcg ttgcagccga cggcggcgcc tgccgccccg gggcggccat gagcggaatg
     1081 cgcgagcgca tcgtctcgct gcaggagcag atggatcgca tcgaatggac cgcggaccgc
     1141 gccgagcagc gccgcctcat ggacctgcac gcgaagcgga tgcgcgaggg catgagggag
     1201 atccgcaacc gcgagctcga gcccgcctgc cgcatggaga tgatgacctc catgatggac
     1261 gtgatgatcc gccaccagca ggcgatggaa gatccggaag agcggcggta gatggtcgag
     1321 gctgcgctcg atccgcgggt ggaggcggaa gcccgggatt gcttccattg cggcctgccg
     1381 gtgcccgagg gcgttcgctt cggattccgc gccgcggaag aatggcgcgc cttctgctgc
     1441 gcgggctgcg aggcggtcgc gcgggtgatc gcgggcgagg ggctcgacga ctactaccgc
     1501 ttgcgctccg ccgctccgcc gcgcctcgaa aggagcgagg cccccgacct cggcctctac
     1561 gaccacgacg cgatgcaggc gcgcttcgtg cgggaagacg cgaacgggat tcgggaagcg
     1621 gagctcgtcg tcgaaggcat tcgctgcgcg gcgtgcgcgt ggctgatcga gaaagccgtc
     1681 gcgcgcgttc ccggcgtcga gtcgctggcg gtgaacgcga cgacccgcaa cgcgcgcctg
     1741 cgatggaatc ccgccctcgg cacgctgagc tcggtattcg gggcgatcca ccgcgtgggg
     1801 taccgcgcct ggcctcgcga gacggccgcc ctcgagctga tcgagaggcg cgagcgcaac
     1861 tccctcctgc ggcggctgtg ggtcgcgggc ctgggcatga tgcaggtgat gatgtacgcc
     1921 gtgcccgcct
//
LOCUS       DASVII010000720         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_285373,
            whole genome shotgun sequence.
ACCESSION   DASVII010000720 DASVII010000000
VERSION     DASVII010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285373"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VF309_09360"
     CDS             complement(<1..1622)
                     /locus_tag="VF309_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7466836.1"
                     /translation="MPDILANLNPEQHKAVTLPRESALILAGAGSGKTRVLTTRIAHL
                     IQGGEASPAGILAVTFTNKAAREMLTRLSAMLPINTRGMWVGTFHGLCNRMLRAHHRD
                     AGLPQLFQILDSGDQQSLIKRLAKAQGLDEEKFPPRQLQNFINNCKEAGLRPNAVEAG
                     DEFTRRMVAFYADYDAQCNREGVVDFAELLLRTFEVLANNLNLLQHYQGRFRYILVDE
                     FQDTNKLQYKWIKMLAGTNGCVFAVGDDDQSIYRFRGAEVGNMNEFVRDFDVKEVVKL
                     EQNYRSQGHILDAANAIIAQNKARLGKNLWTAEGKGEPLRVYAAANDDEEARFIVDEV
                     KALHREGMALSDMALLYRSNAQSRILEHALFRAGLAYRVYGGLRFFERQEVKHALAYL
                     RLAANPDDDTAFSRVVNFPPRGIGARSVEQLQEAGERGMGSLMHVARAGVVTGRSGAA
                     IAQFIAVVDALRKSTETLALAELIEEVLDKSRLKDHYAAEKDGQDRLENLHELINAAA
                     LFAEDFEHGLEPAGEAQEDVADIAGTPEQQILA"
BASE COUNT          296 a          586 c          612 g          343 t
ORIGIN      
        1 gcgaggatct gctgctcggg cgtgcccgcg atgtcggcga cgtcctcctg cgcttcgccc
       61 gcgggctcga ggccgtgctc gaaatcctcg gcgaagagcg ccgccgcgtt gatgagctcg
      121 tgcaggttct cgaggcggtc ctgcccgtcc ttctcggcgg cgtagtgatc cttcaggcgg
      181 ctcttgtcga gcacctcctc gatgagctcc gcgagcgcca gcgtctcggt ggacttgcga
      241 agcgcatcga ccaccgcgat gaattgcgcg atcgccgcgc cgcttcgccc cgtgacgacc
      301 cccgcgcgcg cgacgtgcat gaggcttccc atgccgcgct cgcccgcttc ctggagctgc
      361 tcgacggagc gcgcgccgat cccgcgcggc gggaaattca cgacgcgcga gaacgcggtg
      421 tcgtcgtcgg gattggccgc gaggcgcagg tatgccagcg cgtgcttcac ttcctgccgc
      481 tcgaagaatc gcaagccccc gtagacgcga taggcgaggc ccgcgcggaa gagcgcgtgc
      541 tcgaggatgc gcgactgcgc gttggagcga tagaggagcg ccatgtccga gagcgccatg
      601 ccctcgcgat gcaacgcctt cacctcgtcg acgatgaagc gcgcctcctc gtcgtcgttg
      661 gcggcggcgt agacccgcag cggctcgccc ttgccttccg ccgtccacag gttcttgccc
      721 aggcgcgcct tgttctgcgc gatgatcgcg ttcgccgcgt cgaggatatg gccctgggag
      781 cggtagttct gctcgagctt cacgacttcc ttcacgtcga agtcgcgcac gaactcgttc
      841 atgttgccga cctcggctcc gcggaagcgg tatatcgatt gatcgtcgtc ccccaccgcg
      901 aagacgcagc cgttcgtccc cgcgagcatc ttgatccact tgtactgcag cttgttcgtg
      961 tcctggaatt cgtccacgag gatgtagcgg aagcggccct ggtagtgctg cagcaagttg
     1021 aggttgttcg cgagcacctc gaaggtgcgc agcagcagct cggcgaaatc gaccacgccc
     1081 tcgcggttgc actgcgcgtc gtagtcggca tagaaggcga ccatgcggcg cgtgaactcg
     1141 tcgcccgcct cgaccgcgtt cgggcgaagc cccgcttcct tgcagttgtt gatgaaattc
     1201 tgcagctggc ggggaggaaa tttctcctcg tcgagcccct gcgccttcgc gaggcgcttg
     1261 atgagcgact gctggtcgcc cgaatcgagg atctggaaga gctgcggcag ccccgcgtcg
     1321 cgatgatgcg cgcgcagcat ccggttgcag agcccatgga acgtcccgac ccacatcccg
     1381 cgcgtgttga tcggcagcat cgcggaaagg cgcgtgagca tctcgcgcgc cgccttgttg
     1441 gtgaacgtga ccgcgaggat gccggcgggg ctcgcctcgc cgccctggat gaggtgcgcg
     1501 atgcgcgtgg tgagcacgcg cgtcttgccg ctgcccgcgc ccgccaggat gagagcggat
     1561 tcgcgcggga gggtgacggc cttgtgctgc tcgggattga ggttggcgag aatgtcgggc
     1621 attgctgaaa aaatggtgtc aggtacggat ttcttcaaac cggccaaacg gtcgattttc
     1681 cgggaaaaga acgcacctgg cagcgaattt tcaaaaaaaa agagggcgca tcgcgcgccc
     1741 tcaactgtct gcccttgttg ccatgtcaag acaatgcgtt tgcgcgcgaa gttcccgagg
     1801 aggcgaagga tatcgaggtc atcgcgggga aacgagg
//
LOCUS       DASVII010000721         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_464898,
            whole genome shotgun sequence.
ACCESSION   DASVII010000721 DASVII010000000
VERSION     DASVII010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464898"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF309_09365"
     CDS             <1..213
                     /locus_tag="VF309_09365"
                     /inference="COORDINATES: protein motif:HMM:NF028446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466837.1"
                     /translation="RDAGRMGGTQIAPGGRIERPAAISASNVMLVCPTCKLPTRIGYR
                     FKEGKGGEQIKVRVCKRDGCGQEIDK"
     gene            213..728
                     /gene="rplE"
                     /locus_tag="VF309_09370"
     CDS             213..728
                     /gene="rplE"
                     /locus_tag="VF309_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7466838.1"
                     /translation="MAATETYKPRLKERYNDELRGELREQLELESIMQAPSIAKITLN
                     MGVGEGKTDAKQIDAALEELTTIAGQRAQVRKARKSIAQFKIREGMPIGVKVTLRGDR
                     MWEFLDRLVSIALPRIRDFRGLNPDSFDGRGNYSLGIREQIIFPEIDYDSINQIRGLD
                     VAITTTATNDN"
     gene            788..973
                     /locus_tag="VF309_09375"
     CDS             788..973
                     /locus_tag="VF309_09375"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX7466839.1"
                     /translation="MAKKSLRVKAARPAKFKVRNYTRCNRCGRPRAVFKTFGLCRIGL
                     RELAHQGVIPGMTKSSW"
     gene            982..1377
                     /gene="rpsH"
                     /locus_tag="VF309_09380"
     CDS             982..1377
                     /gene="rpsH"
                     /locus_tag="VF309_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7466840.1"
                     /translation="MLTDPVADMLTRIRNANKALHDNAEMPTSKLKVEIARILKEEGY
                     IRDFHVSDAGTHPTLVVELKYGRGRERVLSGLKRVSKPGRRIYAGKDRRQRVLGGMGI
                     AIMSTSRGVVTSRTAEAEGIGGEVICYVW"
     gene            1384..1917
                     /gene="rplF"
                     /locus_tag="VF309_09385"
     CDS             1384..1917
                     /gene="rplF"
                     /locus_tag="VF309_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7466841.1"
                     /translation="MSRIGKRPIEVPAGVIVSIDPGRVTVSGPKGELRQEVPLRMQVA
                     QEESTITVTRPTERGEDRALHGLTRTLIANMVEGVTQGFEKRLEIQGVGYRAALSGTD
                     LELQVGYSHSVKIKPRQGIEFEVPIPTQVVVRGIDKQAVGQTAAEIRKVRPPEPYKGK
                     GIRYEGEFVRRKVGKRA"
BASE COUNT          366 a          672 c          631 g          285 t
ORIGIN      
        1 cgcgacgccg gccgcatggg cggcacgcag atcgcaccgg gcggacgcat cgagcgcccc
       61 gccgcgatct ctgcctccaa cgtgatgctc gtctgcccca cctgcaagct cccgacgcgc
      121 atcggctacc gcttcaagga aggcaagggc ggcgagcaga tcaaggtgcg cgtctgcaag
      181 cgcgacggct gcggccagga gatcgacaag taatggctgc gacagagacc tataagccgc
      241 gcctcaagga gcgctacaac gacgagcttc gcggcgagct gcgcgagcag ctcgagctcg
      301 agtcgatcat gcaggcgccg tcgatcgcca agatcacgct caacatgggc gtcggcgagg
      361 gcaagaccga cgcgaagcag atcgatgccg ccctcgagga gctgacgacc atcgccggcc
      421 agcgcgcgca ggtgcgcaag gcccgcaagt cgatcgcgca gttcaagatc cgcgagggca
      481 tgcccatcgg cgtcaaggtg acgctgcgcg gcgaccggat gtgggagttc ctcgatcggc
      541 tcgtgtcgat cgcgctcccg cgcatccgcg acttccgcgg cctcaacccc gactcgttcg
      601 acggtcgcgg caactactcg ctgggcattc gcgagcagat catctttccg gagatcgact
      661 acgacagcat caatcagatc cgcggcctcg acgtcgcgat caccacgacc gcgacgaacg
      721 acaactaagc tcgagcgctg ctcaccgcgc tcggcatgcc gttcgccgcc gacggaggca
      781 acaactagtg gcgaagaagt cgctgagagt caaggccgca aggcccgcga agttcaaggt
      841 ccgcaactac acgcgctgca accgctgcgg tcgtccgcgt gccgtcttca agacgttcgg
      901 gctctgtcgt atcggcctgc gggagctcgc ccaccagggc gtcatccccg gcatgaccaa
      961 gagctcctgg taggaggaac tgtgctcacc gatcccgtcg ccgacatgct cacgcgtatc
     1021 cgcaacgcca acaaggcgct gcacgacaac gccgagatgc cgacgtccaa gctgaaggtc
     1081 gagattgccc gcatcctcaa ggaggagggc tacatccgcg acttccatgt ctcggacgcc
     1141 ggtacgcatc cgacgctcgt cgtcgagctc aagtacggcc gcggccgcga gcgtgttctc
     1201 tccggcctca agcgcgtgtc caagccgggc cggcggatct acgccggcaa ggaccgccgc
     1261 cagcgcgtgc tgggcggcat gggcatcgcg atcatgtcca cctctcgcgg cgttgtcacg
     1321 agccgcaccg ccgaagccga aggcatcggc ggcgaagtca tctgctacgt ctggtaacca
     1381 cacatgtctc gcattggtaa aaggcccatc gaagttcccg ccggcgtgat cgtctcgatc
     1441 gaccctggtc gcgtcaccgt gtccgggccg aagggcgagc tgcgccagga ggttcccctg
     1501 cgcatgcagg tcgcgcagga ggagtcgacc atcaccgtca cccgcccgac cgagcgcggc
     1561 gaggatcgcg cgctgcacgg cctgacgcgg acgctgatcg cgaacatggt cgagggtgtc
     1621 acgcagggct tcgagaagcg cctcgagatc cagggcgtcg gctaccgcgc cgcgctttcg
     1681 ggcaccgacc tcgagctgca ggtcggctac tcgcactccg tgaagatcaa gccgcgccag
     1741 ggcatcgagt tcgaggtgcc gatcccgacg caggtcgtcg tgcgcggcat cgacaagcag
     1801 gctgtcggcc agaccgccgc ggagatccgc aaggttcgcc cgcccgagcc gtacaagggc
     1861 aagggcatcc gctacgaggg cgagttcgtt cgccggaagg tcgggaagcg cgcatgagct
     1921 cactcactgt tcgcgaagct cgtctgcgcc gcca
//
LOCUS       DASVII010000722         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_34352, whole
            genome shotgun sequence.
ACCESSION   DASVII010000722 DASVII010000000
VERSION     DASVII010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34352"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /gene="rpoB"
                     /locus_tag="VF309_09390"
     CDS             <1..1974
                     /gene="rpoB"
                     /locus_tag="VF309_09390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7466842.1"
                     /translation="AEKPLVGTGIERTVAIDSGTAVQARRGGRIDYVDAGRIVVRVND
                     NETRPGEAGVDIYNLTKYTRSNQNTNINQRPLVKVGEHIASGDVVADGASTDMGELAL
                     GQNLLVAFMPWNGYNFEDSILISERVVAEDRYTSIHIEELVVVARDTKLGPEEITRDI
                     SNLSERMLSRLDESGIVYIGAEVEAADVLVGKVTPKGETQLTPEEKLLRAIFGEKASD
                     VKDTSLRVPSGINGTVIDVQVFTREGIARDKRAQQIIDEQLKDYRKDLHDQLRIVEDD
                     AFTRIEKVLIGKTAKGGKKLTKGAKVTKAYLTETERHEWFDIRLADEDAAAQLEQLRE
                     SLDLTRKEFDRAFEEKKKKLTQGDELPPGVQKMVKVYVAVKRRLQPGDKMAGRHGNKG
                     VISKIVPVEDMPFMADGTPVDIVLNPLGVPSRMNVGQILETHLGWAAKGLGLRIQEML
                     RVQDNAAKIRKFLERIYNSTGKKEDIASLTEVEVLELAHNLQNGVPFATPVFDGATEI
                     EIKDMLETAGLPRSGQIALFDGRTGDAFDRPVTVGYMHILKLHHLVDDKMHARSTGPY
                     SLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDTQGRTKVYENIV
                     KGEHKIEAGMPESFNVLVKEIRSLAIDIDLERY"
     gene            2027..>2974
                     /locus_tag="VF309_09395"
     CDS             2027..>2974
                     /locus_tag="VF309_09395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163426.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7466843.1"
                     /translation="MKALLDLFKQVTQEEEFDAIRIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLTKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLNRCQLL
                     SEDDYLAKVEEYGDDFSAAMGAEGVRELLRKLDVGHDIETLRTELAATGSETKIKKIA
                     KRLKVLEAFNKSGIKPDWMVMEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAM"
BASE COUNT          619 a          969 c          956 g          430 t
ORIGIN      
        1 gcggagaagc ccctggttgg caccggcatc gagcgcaccg tcgcgatcga ctccgggacc
       61 gcggtccagg cccgtcgcgg cggccgcatc gactacgtcg acgccgggcg catcgtggtg
      121 cgcgtgaacg acaacgagac gcgccccggc gaagccggcg tggacatcta caacctcacc
      181 aagtacacgc gctccaacca gaacaccaac atcaaccagc gtccgctggt gaaggtgggc
      241 gagcacatcg cctccggtga cgtggtggcg gatggtgcct ccacggacat gggcgagctg
      301 gcactcggcc agaacctgct ggtcgcgttc atgccgtgga acggatacaa cttcgaggac
      361 tcgatcctca tctccgagcg cgtcgtcgcc gaagaccgct acacctcgat ccacatcgag
      421 gaactggtgg tggtcgctcg cgacaccaag ctcgggcccg aggagatcac ccgcgacatc
      481 tcgaacctct cggagcgcat gctttcgcgc ctcgacgagt cgggcatcgt ctacatcggc
      541 gcggaagtcg aagccgccga cgtgttggtg ggcaaggtga cgccgaaggg cgagacccag
      601 ctcaccccgg aggaaaagct cctgcgcgcg atcttcggcg agaaagcctc cgacgtgaag
      661 gacacgtcgc tgcgcgttcc gtcgggcatc aacggcaccg tgatcgacgt ccaggtgttc
      721 acccgcgagg ggatcgcccg cgacaagcgt gcccagcaga tcatcgacga gcagctcaag
      781 gactaccgca aggacctgca cgaccagctg cgcatcgtcg aggacgacgc cttcacgagg
      841 atcgagaagg tcctgatcgg caagaccgcg aagggcggca agaagctcac caagggcgcc
      901 aaggtcacca aggcgtacct cacggagacc gagcgccacg agtggttcga catccgcctc
      961 gccgacgagg acgcggccgc ccagctcgag cagctgcgcg agtcgctcga cctcacgcgc
     1021 aaggaattcg accgcgcgtt cgaggaaaag aagaagaagc tcacgcaggg cgatgagctg
     1081 cccccgggcg tgcagaagat ggtcaaggtc tacgtcgcgg tgaagcggcg tctgcagccg
     1141 ggcgacaaga tggccggccg ccacggcaac aagggcgtga tctccaagat cgtgccggtg
     1201 gaagacatgc cgttcatggc cgacgggacg ccggtggaca tcgtgctgaa cccgctgggc
     1261 gtgccctcgc gcatgaacgt gggccagatc ctcgagacgc acctgggctg ggccgcgaaa
     1321 ggcctgggcc tgcgcatcca ggagatgctg cgcgtgcagg acaacgccgc caagatccgc
     1381 aagttcctcg agaggatcta caactcgacc ggcaagaagg aggacatcgc ctccctcacc
     1441 gaggtcgaag tgctggagct cgcgcacaac ctgcagaacg gcgtgccgtt cgcgacgccg
     1501 gtgttcgacg gcgcgaccga gatcgagatc aaggacatgc tggaaacggc ggggctccca
     1561 cgcagtgggc agatcgccct tttcgacggt cgcaccggcg acgcgttcga ccgcccggtg
     1621 accgtgggct acatgcacat cctgaagctg caccacctcg tggacgacaa gatgcacgcg
     1681 cgttcgacgg gaccgtactc cctcgtcacc cagcagccgc tgggaggcaa ggcgcagttc
     1741 ggcggccagc gcttcggcga gatggaagtg tgggcgctgg aagcctacgg cgcggcgtac
     1801 acgctgcagg agatgctcac cgtcaagtcc gacgacacgc agggccgcac caaggtctac
     1861 gagaacatcg tgaagggcga gcacaagatc gaggcgggca tgcccgagtc gttcaacgtg
     1921 ctcgtgaagg agatccgttc ccttgcgatc gacatcgacc tcgagcgcta ttgatagcta
     1981 gtagctggat tccaaaccta gacgctagcc accaggagat tacgtaatga aagcattgct
     2041 cgatctgttc aagcaggtca cccaggaaga agagttcgac gccatccgca tcgggctcgc
     2101 ttcccccgag aagatccgct cgtggtccta cggcgaagtg aagaagccgg agacgatcaa
     2161 ctaccgcacc ttcaagccgg agcgcgacgg cctcttctgc gcgaagatct tcggcccgat
     2221 caaggactac gagtgcctgt gcggcaagta caagcgcctc aagcaccgcg gcgtaatctg
     2281 cgagaagtgc ggcgtggaag tgactctcac caaggtgcgc cgcgagcgca tgggccacat
     2341 cgagctcgcg agccccgtcg cgcacatctg gttcctgaag agcctgccct cgcgcctggg
     2401 catggtgctc gacatgacgc tgcgcgacat cgaacgcgtg ctctacttcg aagcctacgt
     2461 cgtgaccgac ccgggcatga cgccgctgaa ccgctgccag ctcctctccg aggacgacta
     2521 cctcgccaag gtggaggaat acggcgacga cttctccgcg gcaatgggcg ccgagggcgt
     2581 tcgtgagctg ctgcgcaagc tcgacgtcgg ccacgacatc gagacgctgc gcaccgagct
     2641 cgccgccacc ggctccgaga ccaagatcaa gaagatcgcc aagcgcctga aggtgctcga
     2701 ggccttcaac aagtcgggca tcaagcccga ctggatggtg atggaagtgc tgcccgtgct
     2761 gccgccggac ctgcgtccgc tggtgccgct ggacggcggg cgcttcgcga cctccgacct
     2821 gaacgacctc tatcgccgcg tcatcaaccg caacaaccgc ttgaagcgcc tcctcgagct
     2881 gaaagcgccc gagatcatcg tgcgcaacga gaagcgcatg ctgcaggaag ccgtggattc
     2941 gctgctcgac aacggccgcc gcggcaaggc gatg
//
LOCUS       DASVII010000723         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_177102,
            whole genome shotgun sequence.
ACCESSION   DASVII010000723 DASVII010000000
VERSION     DASVII010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177102"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VF309_09400"
     CDS             <1..586
                     /locus_tag="VF309_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466844.1"
                     /translation="PGGTWNAQRTSIAGPVYSPRGSPYSAYDAAKFVPGAAAGEVTLA
                     FQDLNHGTLDYTIAGVPGHKAITRQTFGSVDVTPWPSLGDMYWGGPSQDGWGISVMQQ
                     YRQLFSVWYTYDANGAPTWYVMPQGSWSDAQTCVGHIYRTTGSPWLGRAYDPAKLDVV
                     DVGGFQLRFTPAGASFDYAIDGKGGTMALVRQPF"
     gene            589..993
                     /locus_tag="VF309_09405"
     CDS             589..993
                     /locus_tag="VF309_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466845.1"
                     /translation="MTRFAARFLLPLFVALAALGASAAAPNFDQLEQKLKIRPEQKSQ
                     YDLAIGATKRALLMIGLTAMQLKERLAVELAKPNPDFVALMRSHQEILERSRPQFKEA
                     GEEWKRLYALLDPEQVEIAKAFVRDNVSRFIQ"
     gene            1040..>1824
                     /locus_tag="VF309_09410"
     CDS             1040..>1824
                     /locus_tag="VF309_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7466846.1"
                     /translation="MKRAWAMACVVSLAPWCALAEDAPDKSDKPPAEEQWSVHAQATY
                     IAQIKPAFEAAYSGPVSLTPERAHSYSFTSTLFLGARLWRGGEAYANLEGVEGLPISN
                     LTGLGGLTNGELQKTAGTSLKWYRARAFLRQVIGYEGGPEAIEADKNQLGGNYDKRRL
                     VVTAGNLSVMDVFDDNRYAHDPRTDFMNWSLITSGAYDFAADSRGYSWGAAVEWIDDG
                     WALRAGRFAQPKESNGLPLDFRLFRHYGDQVELERSIELGGRAG"
BASE COUNT          312 a          606 c          618 g          288 t
ORIGIN      
        1 gccgggcggc acgtggaacg cgcagcgcac ttcgatcgcg ggtccggtct actcgccgcg
       61 cggctcgcct tactccgcct acgacgcggc caaattcgtc ccgggcgcgg cggccggcga
      121 ggtgacgctc gcattccagg atctcaacca tgggacgctc gactacacga tcgcgggcgt
      181 gccgggccac aaggcgatca cgcgacagac tttcggctcg gtggatgtca cgccgtggcc
      241 gagtcttggc gacatgtatt ggggcggccc gagccaggac ggttggggca tctcggtgat
      301 gcagcaatat cgccagctct tcagcgtgtg gtacacgtac gacgcgaacg gcgctccgac
      361 ctggtacgtg atgccgcagg gctcgtggag cgatgcccag acgtgcgtgg gccacatcta
      421 tcgcaccacg ggctcgccgt ggctgggccg cgcgtacgac cctgcgaagc tcgatgtcgt
      481 cgacgtgggc ggattccagc tgcgcttcac gcccgcgggc gcgagcttcg actacgcgat
      541 cgacgggaag ggcggtacga tggcgctggt gcgacagccc ttctagccat gacccgattc
      601 gccgcgcgct tcctccttcc cctgttcgtc gcgctcgcgg cgctcggcgc ttcggccgcg
      661 gcgccgaact tcgaccagct cgagcagaag ctcaagatcc gtcccgagca gaagtcgcag
      721 tacgacctcg cgatcggcgc caccaagcgc gcgctgctga tgatcggcct cacggcgatg
      781 cagctgaagg agcgcctcgc cgtcgagctc gcgaagccca accccgactt cgtcgccctc
      841 atgcgctcgc accaggaaat cctcgagcgg agccggccgc agttcaagga agccggcgag
      901 gaatggaaga ggctctacgc cctcctcgat cccgagcagg tcgagatcgc caaggcgttc
      961 gttcgcgaca acgtgagccg cttcatccaa tagtagaaac acgctgcgct atcattccgc
     1021 cagggacctg ggagggacca tgaagagagc atgggcgatg gcgtgcgtcg tctcgcttgc
     1081 gccgtggtgc gcgctcgcgg aagacgcgcc ggataagagc gacaagccgc ccgccgaaga
     1141 gcagtggtcc gtgcatgcgc aagcgactta catcgcgcag atcaagccgg ccttcgaggc
     1201 ggcgtattcc gggccggtga gcctcacgcc ggagcgcgcg cacagctact cgttcacgtc
     1261 gacgctcttc ctcggcgcgc gcctgtggcg cggcggcgag gcctacgcga acctggaagg
     1321 cgtcgaggga ttgcccatct ccaacctgac gggcctcggc ggactgacca acggcgagct
     1381 gcagaagacg gcgggcacgt cgctcaagtg gtatcgcgcg cgcgcgttcc tgcgacaggt
     1441 gatcggctac gaaggcgggc ccgaagcgat cgaggccgac aagaaccagc tcggcgggaa
     1501 ctacgacaag cggcgcctcg tcgtcacggc gggcaatctc tcggtcatgg acgtcttcga
     1561 cgacaaccgc tatgcgcatg acccgcgcac ggatttcatg aactggtcgc tcatcaccag
     1621 cggcgcctac gatttcgccg ccgactcgcg cggatactcg tggggagcgg ccgtggaatg
     1681 gatcgacgac ggctgggcgt tgcgcgcggg tcgattcgcg cagccgaagg aatcgaacgg
     1741 gctgccgctc gactttcggt tgtttcgcca ctatggcgac caggtcgagc tcgagcgctc
     1801 gatcgagctg ggcggccgcg ccgg
//
LOCUS       DASVII010000724         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_393641,
            whole genome shotgun sequence.
ACCESSION   DASVII010000724 DASVII010000000
VERSION     DASVII010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393641"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VF309_09415"
     CDS             <1..578
                     /locus_tag="VF309_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEX7466847.1"
                     /translation="MLALIILYKLGDAFALALSTAFLIRGAGFSPDDVGYVNKGMGLA
                     ATIVGVVFGGALMVRLGLYRALMMFGILQAVSNLAYMWLAMAGTSYPLLILSVGFDNV
                     AGGMGTVAFVALLMALCDHRFTATQYALLSALAAFGRVYVGPAAGFATDPKYLGLSWP
                     TFFFSTFVVALPGLIVLAWKRQTIEALDRKP"
     gene            589..1149
                     /locus_tag="VF309_09420"
     CDS             589..1149
                     /locus_tag="VF309_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466848.1"
                     /translation="MPTKSIMFNAALEPLTQPLPADIAAGVGAIVQRHAYLDWVLGQV
                     MYSLMEISIKQGRVIMKLPRPRVYVAAVKELFEFHSLAARFDFDDLAKCLERAESARR
                     ELTRSVYMRDTDSRALRIELVRSPWDPGPGGDTQPESQSLDARHLARKRKDVEDALKC
                     AAKLRALTDKQLRARHRSRLAASRRK"
     gene            1183..1794
                     /locus_tag="VF309_09425"
     CDS             1183..1794
                     /locus_tag="VF309_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEX7466849.1"
                     /translation="MSNPLENEIAQHVARHLGPPLEMGLRETVGGLSILVVPASQRRP
                     WQTLVTRGMSDHAMDAPAGAEDYRHVELFMRLPPDWPLTREAFDLPDNYWPIESMRLI
                     AHWPETEPVFLASEQTVGNSPPEPFAPGTRLSSMMLLEDPTAFGSLRLADGRVVRFYQ
                     LIPLYEEERAMKERMGTRALIEAFQRNGVSDVIRPDRVNVAAA"
     gene            complement(1826..2410)
                     /gene="metW"
                     /locus_tag="VF309_09430"
     CDS             complement(1826..2410)
                     /gene="metW"
                     /locus_tag="VF309_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX7466850.1"
                     /translation="MQRYDYDLIASWIPERSRVLDLGCGDGVLMAGLAATNAAVGYGI
                     EIDDGNVLASVKNGVNVLQTDIESGLASFEDNSFDFVILSQTLQAMRNAESVLNEMLR
                     VGREGIVTFPNFGYWRHRLDILRGRMPESKALPYKWYDTPNIHLCTVKDFEDLCVTVG
                     AEILDERVLHEGRTVSFLPNLLGSLGVFRFRRKP"
     gene            complement(2410..>2734)
                     /locus_tag="VF309_09435"
     CDS             complement(2410..>2734)
                     /locus_tag="VF309_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466851.1"
                     /translation="LARALSAARAGFFVASFKSDWRFPPQRSRELVKALVASKKAVTY
                     AEIDAPHGHDAFLLDDARYHQLIAAYVDNIAVEIGVGDRAAPLQLAKGEIEEMSAYAS
                     ERGGS"
BASE COUNT          422 a          943 c          902 g          467 t
ORIGIN      
        1 cgatgctcgc gctcatcatc ctctacaagc tgggagacgc cttcgcgttg gccctctcca
       61 cggcgttcct cattcgcggc gccggctttt cgcccgacga cgtgggctat gtgaacaaag
      121 gcatgggcct cgccgccacg atcgtgggcg tcgtgttcgg cggcgcgctg atggtgcggc
      181 tgggacttta tcgcgcgctc atgatgttcg gaatcctgca ggcggtctcg aacctcgcct
      241 acatgtggct cgcgatggcg ggcaccagct atccgctgct gatcctttcg gtcgggttcg
      301 acaacgtcgc gggcggcatg ggcaccgtcg cattcgtggc gctcctcatg gcgctctgcg
      361 accatcgctt caccgcgacg caatacgcgc tgctttcggc gctcgccgcg ttcgggcgcg
      421 tctacgtcgg ccccgcggcg ggtttcgcga ccgatccgaa atacctcggc ctttcgtggc
      481 ccacgttctt cttctcgacc ttcgtggtcg cccttcccgg gttgatcgtg ctcgcctgga
      541 agcgtcagac tatcgaggcg ctggaccgca agccgtagga gttaacccat gcccacgaaa
      601 tccatcatgt tcaacgcggc gctcgagccc ctcacgcagc cgcttccggc cgacatcgcg
      661 gccggcgtgg gcgcgatcgt ccagcgccac gcctatctcg actgggtcct cggccaggtg
      721 atgtacagcc tgatggagat ctccatcaag caggggcgcg tgatcatgaa gttgccgcgc
      781 ccgcgcgtct atgtcgcggc cgtgaaggag ctcttcgaat tccacagcct cgcggcgcgt
      841 ttcgatttcg acgacctcgc gaagtgcctc gagagagcgg agagcgctcg ccgggagctc
      901 acgcgttcgg tgtacatgcg cgacaccgat agccgggcgc tgcgcatcga gctcgtgcga
      961 agcccctggg atcccggtcc cggcggcgac acccagcccg agtcccaatc gctcgacgcc
     1021 cggcacctcg cgagaaagcg caaggatgtc gaggacgcgc tcaagtgcgc cgcgaagctt
     1081 cgcgccctga ccgacaagca gctgcgcgcc cggcaccgct cgcggctcgc cgcgtcgagg
     1141 cgcaagtagg cgccgctccg tcacccgaac cggacgaatt ccttgagcaa tcctctcgag
     1201 aacgagatcg cgcagcacgt cgcgcggcac ctaggcccgc cgctcgaaat ggggctgcgc
     1261 gagaccgtgg gaggcctgag catcctggtc gttccggcgt cgcaacgccg gccgtggcaa
     1321 acgctcgtca cgaggggcat gagcgatcac gccatggacg cgcccgccgg cgccgaggac
     1381 taccggcacg tcgagctctt catgcgcctg ccgcccgatt ggccgctcac gcgtgaagcg
     1441 ttcgacctgc cggacaacta ctggcccatc gagtccatgc gcctcatcgc ccactggccc
     1501 gagacggagc cggttttcct cgcgagcgag cagaccgtcg gaaactcgcc gcccgagccg
     1561 ttcgcgccgg gcacgcggct gtcgagcatg atgctgctgg aggacccgac ggcgttcggg
     1621 tcgctgcgcc tcgcggacgg gcgcgtcgtg cgcttctacc agctcattcc cctctacgag
     1681 gaggagcgcg cgatgaagga gcgcatgggc acgcgagcgc tgatcgaggc tttccagagg
     1741 aacggcgtga gcgacgtcat tcgtcccgat cgcgtcaacg tcgccgccgc ttgagcggcc
     1801 gcgccatcgc ccgcgctgaa ggcgatcagg gcttcctgcg aaagcgaaac acgccgaggc
     1861 tgccgaggag gttcggcagg aaggaaacgg tgcggccttc gtggagcacg cgctcgtcga
     1921 ggatctcggc ccccaccgtc acgcacaggt cctcgaaatc cttcaccgtg cagaggtgga
     1981 tgttgggcgt gtcgtaccac ttgtagggga gcgccttcga ttcgggcatg cggccgcgca
     2041 ggatgtcgag gcggtggcgc cagtagccga agttggggaa cgtcacgatc ccctcgcgcc
     2101 ccacgcgcag catctcgttc aggacgctct cggcgttgcg catcgcctgg agcgtctgcg
     2161 agaggatcac gaagtcgaag ctgttgtcct cgaagctcgc caggccgctc tcgatgtccg
     2221 tctgcaggac gttgacgccg ttcttcacgc tcgcgagcac gttcccgtcg tcgatctcga
     2281 tgccgtagcc caccgccgcg ttggtcgcgg cgaggccggc catgagcacg ccgtcgccgc
     2341 agcccaggtc gaggacccgc gagcgctccg ggatccacga ggcgatgaga tcgtagtcgt
     2401 agcgttgcat caggagcctc cccgctcgct cgcgtaggcg ctcatctcct cgatctcgcc
     2461 cttcgcgagc tgcagcggtg cggcgcgatc gccgacgccg atctccacgg cgatgttgtc
     2521 gacgtacgcc gcgatgagct ggtggtagcg cgcgtcgtcg aggaggaagg cgtcgtggcc
     2581 gtggggcgcg tcaatctccg cgtaggtgac cgccttcttc gaggcgacga gggccttcac
     2641 cagctcgcgc gagcgctgcg gcggaaagcg ccagtcgctc ttgaaggacg cgacgaagaa
     2701 tcccgcccgc gccgccgata gcgcccgcgc cagg
//
LOCUS       DASVII010000725         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_501224,
            whole genome shotgun sequence.
ACCESSION   DASVII010000725 DASVII010000000
VERSION     DASVII010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501224"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VF309_09440"
     CDS             complement(<1..1709)
                     /locus_tag="VF309_09440"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX7466852.1"
                     /translation="MLTLVSFEMKTRIALGMALLAAAASARAIEAYRLEAGEKIVLDG
                     RLDEPAWSRAKPWDRFYEVSPDEKIEAKVRTEARFAYDAHALYAGVRAYDPAMAELRA
                     PFARRDNVLADQDMVILFIDPVGKRKFAHFVRANPRGVIADGLYNEDTGNEDFSPDFD
                     VEVVTGRFEGGWIAEFRIPFSSLRYSDPPSKEWSVLVFRNWPRDQRYRMASSPLPRDM
                     TCFLCLNEPLTGLGELPPTRFLTLTPNLTLRSVENRDIGIPARREGDVVASLDIKWRP
                     RADLVADATINPDFSQVELDTPQLAGNAQFALYYPEKRPFFLEGSDILQGPFNAIYTR
                     SVTDPAWGVRATQRRDDFDGTVLVTRDEGGGQVLLPNAYGTNFVPQDFKSTASFARGR
                     WQVAGASVGALFTDRTIDGGAYNRVAGPDVTWFPTTEHRLRAQVLGSWTTALPSGDSL
                     VKGPLQASHAATFDWAYNGARWIEYLKVEDVGREFRADNGFFGQNGYRTVYNETTRKF
                     LNAWGFNEVSPYFFAQYTTVPGGKVQYQQNNFGVRWGLPRATTISTEIRPNNLVAVRE
                     AGGL"
     gene            1819..>3303
                     /locus_tag="VF309_09445"
     CDS             1819..>3303
                     /locus_tag="VF309_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX7466853.1"
                     /translation="MTSPTASVHASPAQRRATAALVVGAIGVVFGDIGTSPLYALKTA
                     FNPEYGIPLTPDNVMGILSLIVWSMVWVITIKYLVVMMRADNNGEGGILALLALALRE
                     VKDRPRLKWTIIGIGMFGAAMFYGDSMITPAISVLSAVEGLEVATPVFAKFVIPITLA
                     VLAGLFFIQSHGTSKVGAWFGPVTVLWFAAIGALGAAQLVKNPAVLAALQPAYAVDFV
                     FAYPLLAFFVLGAVFLAMTGGEALYADMGHFGKKPIRIAWMGLVFPSLLLNYFGQAAF
                     VLANPAAIKNPFYLMVPQWGLYPMVVLATAATVIASQAAISGAFSMTRQAIQLGYIPR
                     LEILHTSERSIGQIYVPFVNWVLFIAVCLLVLGFETSDNLANAYGIAVAATMVIECLL
                     AMVVARLMWKWSMWMVALVIGSMLVVDLLFLASNATKFLSGGWFPVLIGSAIFVLLMT
                     WKRGRALMFRRLTEQGIPLKPFIEGLHAHPPQKVQGTAIFMTANV"
BASE COUNT          503 a         1101 c         1110 g          589 t
ORIGIN      
        1 aggccgcccg cctcgcgcac cgcgacgagg ttgttggggc ggatctccgt ggagatcgtc
       61 gtcgcgcggg gcaggcccca cctcacgccg aagttgttct gctggtactg cacctttccg
      121 ccgggcacgg tcgtgtattg cgcgaagaag tagggcgaca cctcgttgaa cccccaggca
      181 ttgaggaact tgcgcgtggt ctcgttgtag acggtgcggt agccgttctg cccgaagaag
      241 ccgttgtcgg cgcggaattc gcgccccacg tcctcgacct tgaggtactc gatccaccgc
      301 gcgccgttgt aggcccagtc gaacgtcgcg gcgtggctcg cctgcaatgg gcctttcacc
      361 agggaatcgc ctgaaggaag cgccgtggtc caggagccga gcacctgcgc gcgcagccga
      421 tgctcggtcg tcggaaacca cgtgacatcc ggaccggcga cgcggttgta cgcgccgccg
      481 tcgatcgtgc ggtcggtgaa gagcgcgccc acgctggcgc cggccacctg ccagcgcccg
      541 cgcgcgaagc tcgccgtcga cttgaaatcc tgcgggacga agttggtgcc gtacgcgttg
      601 ggcaggagca cctggccgcc gccctcgtcg cgcgtcacga gcacggtccc gtcgaagtcg
      661 tcgcggcgct gggtcgcccg cacgccccac gcgggatcgg tgaccgagcg ggtgtagatc
      721 gcgttgaacg gcccctgcag gatgtcggag ccctcgagga agaagggccg cttctccggg
      781 tagtagagcg cgaactgcgc gttgcccgcg agctgcggcg tgtcgagctc gacctgcgag
      841 aagtcgggat tgatggtcgc gtccgcgacg aggtcggcgc gcgggcgcca cttgatgtcg
      901 aggctcgcga ccacgtcgcc ctcgcgccgc gccgggatgc cgatgtcgcg gttctcgacg
      961 ctgcgcaacg tgaggttcgg cgtgagcgtg aggaatcgcg tcgggggaag ctcgcccagc
     1021 ccggtgagcg gctcgttcag gcagaggaag cacgtcatgt cgcgcggcag cgggctcgag
     1081 gccatgcggt aacgctggtc gcgcggccag ttgcggaaga cgagcacgct ccattccttc
     1141 gacggcgggt cgctgtagcg aagcgaggaa aacgggatgc ggaattccgc gatccaaccg
     1201 ccctcgaagc gccccgtcac cacctcgacg tcgaagtccg gcgagaaatc ctcgttgccg
     1261 gtgtcctcgt tgtagagccc gtcggcgatc acgcccctcg ggttggcgcg cacgaaatgc
     1321 gcgaacttgc gcttgcccac cgggtcgatg aagaggatga ccatgtcctg gtcggccagg
     1381 acgttgtcgc gccgcgcgaa gggcgcgcgc agctcggcca tggcgggatc gtaggcgcgc
     1441 acgcccgcgt agagcgcgtg ggcgtcgtag gcgaagcgcg cctcggtgcg gaccttggcc
     1501 tcgatcttct cgtcgggcga gacctcgtag aagcggtccc agggcttggc gcgggaccat
     1561 gccggctcgt cgaggcgacc gtcgaggacg atcttctcgc cggcctcgag ccgataggcc
     1621 tcgatggcgc gcgccgacgc ggcggccgcg agaagcgcca tccccagtgc gattcttgtt
     1681 ttcatttcga acgacacgag tgtgagcatt attgacgcct gcaccgcggg attcaaagtt
     1741 gtgcgccgca acaaaggggc gtatagtccg cgcgcactcc gccttggatg cacaagcatg
     1801 ctcggcggat tcaaaacaat gacatccccc accgcttcgg ttcacgcctc acccgcgcag
     1861 cgaagggcga cggccgcgct cgtggtcggc gccatcggcg tcgtgttcgg cgacatcggc
     1921 accagcccgc tctacgcgct gaagaccgcg ttcaaccccg agtacgggat cccgctcacg
     1981 ccggacaacg tgatggggat cctgtcgctc atcgtgtggt cgatggtgtg ggtcatcacc
     2041 atcaagtatc tcgtcgtgat gatgcgcgcc gacaacaacg gcgaaggcgg catcctcgcg
     2101 ctcctcgcgc tcgctcttcg cgaggtgaaa gaccgcccgc gactgaagtg gaccatcatc
     2161 ggcatcggca tgttcggcgc ggcgatgttc tacggcgaca gcatgatcac gcccgcgatc
     2221 tcggtgctct ccgcggtcga aggcctcgag gtcgccacac ccgtgttcgc gaagttcgtg
     2281 atcccgatca cgctcgcggt gctcgcgggc ctgttcttca tccagagcca cggcacgagc
     2341 aaggtcggcg cctggttcgg gcccgtcacg gtgctgtggt tcgccgcgat cggcgccctg
     2401 ggcgccgcgc agctggtgaa gaacccggcg gtgctcgccg cgctgcagcc cgcgtacgca
     2461 gtggatttcg tgttcgccta tccgctgctc gccttcttcg tgctgggcgc ggttttcctc
     2521 gccatgaccg gcggcgaggc gctctacgcg gacatgggcc acttcggcaa gaagccgatc
     2581 cgcatcgcct ggatggggct cgtattccca tcgctgctgc tcaactactt cggccaggcc
     2641 gcgttcgtgc tcgccaaccc cgcggcgatc aagaacccct tctacctcat ggtgccgcag
     2701 tggggtctct atccgatggt tgtgctcgcg acggcggcca ccgtcatcgc gtcgcaggcg
     2761 gcgatctcgg gcgcgttctc gatgacgcgc caggcgatcc aactcggcta catcccgagg
     2821 ctcgagatcc tgcacacgtc ggaacgctcc atcggccaga tctacgtgcc gttcgtgaac
     2881 tgggtcctgt tcatcgccgt gtgcctgctc gtgctcggct tcgagacctc ggacaacctc
     2941 gccaacgcct acggcatcgc ggtcgccgcg accatggtga tcgagtgcct gctcgcgatg
     3001 gtggtggcgc gcctcatgtg gaaatggagc atgtggatgg tggcgctggt gatcggaagc
     3061 atgctcgtgg tcgacctcct cttcctcgcc tccaacgcca ccaagttcct ttccggcggc
     3121 tggttcccgg tgctgatcgg ctcggcgatc ttcgtgctgc tgatgacctg gaagcgcggc
     3181 cgcgcgctca tgttccgccg cctgacggag cagggcatcc ccttgaagcc cttcatcgag
     3241 ggccttcacg cgcacccgcc gcagaaggtc cagggcaccg cgatcttcat gacggcgaac
     3301 gtg
//
LOCUS       DASVII010000726         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_537094,
            whole genome shotgun sequence.
ACCESSION   DASVII010000726 DASVII010000000
VERSION     DASVII010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537094"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /locus_tag="VF309_09450"
     CDS             <1..2205
                     /locus_tag="VF309_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7466854.1"
                     /translation="EKMLGYTAAEVMNKITPADISDPQEVIARAKALSIELGTPITPG
                     FEALVFKAARGIEDIYELTYIRKDGSRFPAVVSVTALRDAQDAIIGYLLIGTDNTARK
                     LVEAEQKKLDQRLRDQQFYTRSLIESNIDALMTTDPAGIITDVNKQMEALTGCTRDEL
                     IGAPFKSYFTDPERAEAAIKLVLSEKKVTDYELTARALDGKKTVVSYNATTFYDRGRT
                     LQGVFAAARDVTERKREEAELREAKAMAESASRTKSDFLASMSHEIRTPIHAIIGIAH
                     LLAQTPLSPVQDKYVQIFRRAGDNLLHIVNDILDLSKVEASQLELERTAFSLGDLLGK
                     VAEMVAVRAQEKGLVLACEIAPGVPVDRMGDPTRLRQVLLNLLGNAIKFTESGEVTLR
                     VTPASDSSPGALRFTISDTGIGIPGEKLGAVFERFTQADSSITRSYGGSGLGLTISKS
                     LVELMGGRIWVESEVGKGSVFSFTVPLEIGAAPLPRPPTAIDTAPELPLPALRILLVE
                     DSADNRTITVAYLRDTPYRVDIAENGAIACEKFAAGEYDLVLMDRQMPIMDGLTATRA
                     IRRWEIANDRAPTPIIALTAAALKGDREKCLAAGCTAFLTKPIKQEMLLLAIKERSAS
                     MPLFSQDESNRKDIFIVRANPKFADLIPAFLQNRRQDVVEMTQALERGDFQTVQRLGH
                     DMKGVGDSYGFKAITDIGARLEEAGASVNAGTSRMWMGELSRYLEGVEVVAD"
     gene            2237..>2419
                     /locus_tag="VF309_09455"
     CDS             2237..>2419
                     /locus_tag="VF309_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466855.1"
                     /translation="MSVSSRFRRFLVALPATMIACFALTALAADEDFPTLTKRLQAER
                     PQFAQRQQAMLAQRYDL"
BASE COUNT          483 a          761 c          788 g          387 t
ORIGIN      
        1 gaaaaaatgc ttggttacac cgccgccgag gtgatgaaca aaatcacgcc agccgacatc
       61 tccgatccac aggaagtgat cgcgcgcgca aaggcattga gcatcgagct gggcactccg
      121 attacgccag gctttgaggc attggtgttc aaggccgcgc gcgggatcga ggacatctac
      181 gagctgacct acatccgcaa ggatgggagc cgcttcccgg cagtggtgtc cgtcacggcg
      241 ctgcgcgacg cgcaggacgc catcatcggt tatctcttga ttggtaccga taacaccgcg
      301 cgcaagctgg tcgaggccga gcaaaagaag ctcgatcagc gcctacgtga ccagcagttc
      361 tacacgcgct cgctgatcga atccaacatc gacgcgctga tgaccaccga ccctgccggc
      421 atcatcaccg acgtcaacaa gcagatggag gcgctcaccg gctgcacgcg cgacgagctg
      481 atcggcgcgc cgttcaagag ctacttcacc gatccggaac gggccgaagc ggcgatcaag
      541 ctcgtgctga gcgaaaagaa ggtcaccgat tacgagctga cggcgcgtgc cctcgacggg
      601 aagaaaacgg tggtgtccta caacgcgacc accttctacg accggggccg cacgctgcag
      661 ggcgtcttcg ccgcggctcg cgacgtgacc gagcgcaagc gcgaggaagc ggagctgcgc
      721 gaagccaagg ccatggcgga gagcgcgagc cggaccaagt cggatttcct cgccagcatg
      781 agccatgaga tccgcacgcc gatccacgcg atcatcggga tcgcgcacct cctcgcccaa
      841 accccgctct ccccggtgca ggacaagtac gtgcagatct tccggcgcgc gggggacaac
      901 ctcctgcaca tcgtcaacga catcctcgac ctctccaagg tcgaagcgtc gcagctcgag
      961 ctcgagcgga cggcgttctc gctgggcgac ctcctgggga aagtggccga gatggtggcg
     1021 gtccgcgccc aggagaaagg gctcgtcctc gcgtgcgaga tcgcccccgg agtgcccgtg
     1081 gaccggatgg gcgatccgac ccggttgcgg caggtgcttc tcaatttgct gggcaacgcg
     1141 atcaagttca ccgaatccgg cgaagtgacg ttgcgagtga cgcccgccag cgactcttca
     1201 ccgggcgcgc tgcgattcac gatctcggat acgggcatcg gcattccggg cgagaagctg
     1261 ggcgcggtgt tcgagcgctt cacccaggcc gactcttcca tcacccgcag ctatgggggc
     1321 tcggggctgg ggctgaccat ttcgaagagc ctggtggaac tgatgggagg gcgcatctgg
     1381 gtcgagagcg aggttgggaa gggcagcgtg ttttccttca ccgtgccgct cgagatcggc
     1441 gccgcgccgt tgccgcggcc gcccacggcg atcgacacgg ctcccgagct gccgctgccg
     1501 gcgttgcgca ttctcctcgt cgaggactcc gcggacaatc gcacgatcac cgtggcctac
     1561 ctgcgggata cgccctatcg ggtcgacatc gcggagaacg gcgccatcgc ctgcgaaaaa
     1621 ttcgcggccg gcgagtacga cctggtcctg atggaccggc aaatgccgat catggacgga
     1681 ttgaccgcga cgcgcgcgat caggaggtgg gagatcgcca acgaccgggc gcccacgccc
     1741 atcatcgcgc tgaccgcggc cgcattgaag ggagaccggg aaaagtgcct ggcggcggga
     1801 tgcacggcgt tcctgaccaa gcccatcaag caggagatgc tgctgctggc catcaaggag
     1861 cggtcggcat cgatgccgct attctcgcaa gacgagagca acaggaagga catcttcatc
     1921 gttcgcgcga atccgaaatt cgccgacctg atcccggcat tcctgcagaa ccgaaggcag
     1981 gacgtcgtcg agatgacgca ggccctggag cgcggggact tccagaccgt gcagaggttg
     2041 gggcacgaca tgaagggcgt tggagactcc tacggcttca aggccatcac cgacatcggc
     2101 gcccgcctcg aggaggccgg cgcgagcgtc aacgcgggga cgtcgcgcat gtggatgggc
     2161 gaattgtcca ggtaccttga gggtgtcgag gtggttgcgg actaagccaa aaggctcgta
     2221 agtagaagga ggacgcatgt cagtcagctc tcggttccgc cggttccttg tcgcattgcc
     2281 cgctacgatg attgcttgct tcgcgctgac agcgttggcc gccgatgaag actttccgac
     2341 gctgacaaaa cgccttcaag cggagaggcc gcagttcgcg cagcgccagc aggctatgct
     2401 cgcgcagcgc tacgacctc
//
LOCUS       DASVII010000727         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_34561, whole
            genome shotgun sequence.
ACCESSION   DASVII010000727 DASVII010000000
VERSION     DASVII010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34561"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VF309_09460"
     CDS             <1..572
                     /locus_tag="VF309_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466856.1"
                     /translation="PAASGSATRDALTLKYLDGGGDGCTYPGEKPSFARRNFHHVTFY
                     GFMLCFAATSVATLYHYAFGWKAPYGFWSLPVLLGTAGGLGLLVGPAGLIWLKRRRDA
                     LLVDPSQDGMDLAFLALLFLVSLSGLLLLALRETRAMGIILAVHLGAVLALFLSLPYG
                     KFVHALYRFAALTRFHIERRRPLPEIGSE"
     gene            584..1375
                     /locus_tag="VF309_09465"
     CDS             584..1375
                     /locus_tag="VF309_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7466857.1"
                     /translation="MSNLAQHLTLKSMKDKVSAAEWKARVDLAACYRLVAHYDMSDMM
                     ANHISAAVPGEAGAFLINAYGMMYEEITASSLIKIDIDGNILAKPDFGELDYGINKAG
                     YVIHGAIHRARHDVACVIHTHTWAGMALSALDVGVLPITQTSMRFLRIAYHDYQGVVL
                     DSKEEESLLRDLGTGEALILRNHGLLTVGKTIGEAFNWMHRLELTARSQLAAMATGAK
                     LVSVPDEVLQETYMNYQPQTRRPYGVMEWPALLRKLDRMDPSYRE"
     gene            complement(1391..1672)
                     /locus_tag="VF309_09470"
     CDS             complement(1391..1672)
                     /locus_tag="VF309_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX7466858.1"
                     /translation="MSDEVTMRLVIRGRVQGVWYRESMRLEAERLGVRGWVANRTDGS
                     VEAVVRGTPEQVEAITRWARRGPEQAEVTEVVAAEASGEFSGFEKRATF"
     gene            complement(1665..2033)
                     /locus_tag="VF309_09475"
     CDS             complement(1665..2033)
                     /locus_tag="VF309_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7466859.1"
                     /translation="MPRAPLHPPPYSTDRDILEREVLVDVFRASGPGGQHVNKTESAL
                     RLTHPPSGVVVRAQDSPSQFRNREIAFERLIEHLKRLNHVPKKRVATKPSRAAKRRRI
                     EAKKLRTVVKRTRGKVRGDE"
     gene            2177..2371
                     /locus_tag="VF309_09480"
     CDS             2177..2371
                     /locus_tag="VF309_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466860.1"
                     /translation="MTTKQQLATGFLAMVIVGSIAFMPVNAGNKSAPVPAAQAPSGNM
                     VATVTTPELPQEQVKDLTYN"
BASE COUNT          384 a          828 c          778 g          398 t
ORIGIN      
        1 cgcccgccgc gtcgggaagc gccacccggg acgcgctgac cctcaagtac ctcgacggcg
       61 gcggcgacgg ctgcacctat ccgggcgaga agccctcgtt cgcgcggcgc aatttccacc
      121 acgtcacgtt ctacggcttc atgctgtgct tcgccgcgac ctcggtcgcg acgctctatc
      181 actacgcctt cgggtggaag gcgccgtacg ggttctggag cctgccggtg ctgctcggca
      241 ccgcgggggg cttgggcctg ctcgtcggac ccgcgggcct catctggctc aagcgccggc
      301 gcgatgcgct cctcgtcgat ccctcgcagg acgggatgga tctcgccttt ctcgcgctgc
      361 tcttcctggt cagcctcagc ggattgctgc tcctcgcgtt gcgcgagacg cgcgcgatgg
      421 ggatcattct cgcggtgcac ctcggcgcgg tgctcgcgct gttcctgagc ctgccctacg
      481 gaaaattcgt gcacgcgctc tatcgcttcg cggcgctcac gcgcttccac atcgagcggc
      541 gccgcccgct cccggaaatc ggatcggagt aggagacagc gcaatgtcga acctggccca
      601 gcacctcacc ctgaagtcga tgaaggacaa ggtcagcgcc gcggaatgga aggcgcgcgt
      661 ggacctcgcc gcgtgctatc gcctcgtcgc ccactacgac atgtcggaca tgatggcgaa
      721 ccatatttcc gcggcggttc ccggggaggc gggcgccttc ctcatcaacg cgtacgggat
      781 gatgtacgag gagatcacgg cctccagcct catcaagatc gacatcgacg gcaacatcct
      841 cgcgaagccc gacttcggcg agctggacta cggcatcaac aaggcgggct acgtgatcca
      901 cggcgcgatc caccgcgcgc gacacgacgt ggcgtgcgtg atccacaccc acacctgggc
      961 gggcatggcg ctctccgcgc tcgacgtggg cgtgctgccg atcacgcaga cttcgatgcg
     1021 ctttctcagg atcgcctatc acgattacca aggcgtggtg ctcgacagca aggaagagga
     1081 gtcgttgctg cgcgacctgg gcacaggcga ggccctcatc ctgcgcaacc acggcctcct
     1141 caccgtcggc aagaccatcg gcgaggcgtt caactggatg caccgcctcg agctcaccgc
     1201 gcgctcgcag ctggccgcca tggcgaccgg ggcgaagctg gtgagcgtcc cggacgaggt
     1261 gctgcaggaa acctacatga actaccagcc gcagacgcgc cgtccctacg gcgtcatgga
     1321 gtggcccgcg ctgctgcgca agctcgaccg catggatccc agctaccggg aatgacgggg
     1381 gcgtcgcgcg ctagaaagtc gcgcgcttct cgaagccgga gaattccccc gaggcctcgg
     1441 ccgcgacgac ctcggtcacc tcggcctgct cggggccgcg acgcgcccag cgcgtgatcg
     1501 cctcgacctg ctcgggggtt ccccgcacga ccgcttccac gctgccgtct gtgcggttgg
     1561 ccacccatcc gcgcacgccg agccgctcgg cctccaggcg catcgactcg cggtaccaga
     1621 cgccctgcac gcggccgcgg atcacgaggc gcatcgtcac ctcgtcactc atcgccgcgc
     1681 accttgccgc gcgtgcgctt cacgacggtg cgcagcttct tcgcctcgat gcggcgccgc
     1741 ttggccgcgc gcgaaggctt ggtcgccacg cgcttcttgg ggacgtggtt gaggcgcttc
     1801 aggtgctcga tgaggcgctc gaaggcgatc tcgcggttgc ggaactgcga aggcgagtcc
     1861 tgcgccctca cgacgacgcc ggaaggcggg tgggtcagcc gcagcgccga ttcggtcttg
     1921 ttcacgtgct gcccgccggg cccgctcgcg cggaagacgt cgaccagcac ctcgcgctcg
     1981 aggatgtcgc gatcggtcga gtagggcggc gggtggagag gggcgcgggg catggcggct
     2041 tctcggttga ttcttacaag tgtatttccc cgcggcggcg ttttgtgacc gccttcacag
     2101 gcatgcgtga ccgttcggag aaagatgggc tccatggaca agcgggcgga taagcccgat
     2161 gacgggagac cacactatga ccacgaagca gcaactcgca acgggctttc tcgcaatggt
     2221 gatcgtgggc agtatcgcgt tcatgccggt caacgccggc aacaagagcg ctccggtccc
     2281 cgcggcgcag gccccgtccg gtaacatggt ggccacggtc accacacccg agcttccgca
     2341 ggagcaggtc aaggacctga cctacaacta attcgaggtt tccccgca
//
LOCUS       DASVII010000728         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_213491,
            whole genome shotgun sequence.
ACCESSION   DASVII010000728 DASVII010000000
VERSION     DASVII010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213491"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VF309_09485"
     CDS             complement(<1..1193)
                     /locus_tag="VF309_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7466861.1"
                     /translation="MTMYLEHFGLREAPFRITPHTEFFFSGANRGATLEALLYAITAG
                     EGMVKVTGEVGSGKTMLCRVLMERLPETVETIYLAVPSLSRDEMLAAIANDLGIDTTG
                     ANTTRLIRSLQDKLIDVHSAGRQVVALIDEAHAMPLATLEEIRLLSNLETGTEKLLQI
                     VLFGQPELDAHLALEHMRQLKERITHGFTLAPLPPREVKDYLNFRLRAAGYHGPDLFG
                     PEVLRIINEASEGLTRRINIYADKTLLAAFAAGTHSVTADHARAAVTDTQIVLTRRES
                     PRRLAAAAALGAAAGMVIGFTLAQFVGAPSPVAAVAASSPASAKAAPIAAAAPVTAPA
                     PGTTPGEPGSRVATRLAAGRELLASDSPRYAVQLMVTDAREHGYLENYLVEAARSLRP
                     EQLYLV"
     gene            complement(1236..2660)
                     /locus_tag="VF309_09490"
     CDS             complement(1236..2660)
                     /locus_tag="VF309_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7466862.1"
                     /translation="MKKPRLLLVDDDPLIVESLAFALAGQYEVTVAADRVAAIAALRA
                     GLRPDLALVDLGLPPVAHLPNEGFKLIGDLLAHAPEVRILVLTGQNEESNARRARALG
                     AVELVPKPCEPEYLRSILERAYKSRALDRREDEAEDVKALIGESPPLRRLRSQIDLYA
                     SSAFPALIEGESGSGKERVAFSLHHLSARAKAPFLALNCAAISPALVEPTLFGYAKGA
                     FTGAQQAKSGYFEDAGEGTLFLDEIGELPGELQAKLLRVLENGEYQRVGETSTRVSRA
                     RIVAATNRDLRDEVKHGRFRSDLYHRLSVFELRVPPLRELGDDKLRLLDHYRALYAAK
                     TGSEAFAFDEAARARWMSYPFPGNVRELKNIVIRLTTKYAGYTVAARDLEAEFAPADA
                     SRADPLAAARAELLSGKPFSLDAALKCVEQAYLDAAIEIAQGNMSQAAKMLGVSRSTL
                     YGRLEAAGRSGAKLASPDPGEARG"
     gene            2733..3554
                     /locus_tag="VF309_09495"
     CDS             2733..3554
                     /locus_tag="VF309_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466863.1"
                     /translation="MSEEENLVDVESVNFAYGRRPILKGISMRVQRGKVVAIMGASGS
                     GKTTLLRLIGGQLRPSSGTVRVDGALISELDDEALYRERRKMSMLFQFGALFTDMSVF
                     ENVAFPLREHTQLPEEMIRDLVLMKLEAVGMRGARRLRPAELSGGMARRVALARAMVL
                     DPRLVMYDEPFAGLDPIALAVIGQLIRKLNDALGSTTIIVTHDVVESLQIVDYLYFIS
                     DGVIVGEGTPEEIRRSELPYVKQFVHGEIDGPVPFHYPARPYPDDFALASGGARA"
     gene            3556..>3934
                     /locus_tag="VF309_09500"
     CDS             3556..>3934
                     /locus_tag="VF309_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7466864.1"
                     /translation="MRRPLARLGANVINGIWRAGTMARFFALVLASSATSFGRFRLVM
                     REVYFSGVLSLIIIIVSGLFIGMVLGLQGYQTLQRYGATESIGVLVALSLVRELGPVV
                     SAILFAARAGSAITAEIGLMKTTE"
BASE COUNT          563 a         1350 c         1389 g          632 t
ORIGIN      
        1 accaggtaca gctgctcggg acgaagcgag cgcgccgcct ccacgaggta attctcgaga
       61 tagccgtgct cccgcgcgtc ggtgaccatg agctgcaccg cgtaacgcgg cgagtcggag
      121 gcgagaagct cgcggcccgc ggcaaggcgc gtggcgacgc gcgagccagg ctcgcccggc
      181 gtcgtgccgg gcgccggcgc cgttacgggg gccgccgctg cgatgggcgc cgccttcgcg
      241 gacgcgggcg atgaagcggc gacggcggca accggggagg gtgcgcccac gaactgcgcg
      301 agggtgaacc cgatcaccat ccccgccgcg gcgccgaggg ccgcggcggc ggccaggcgg
      361 cggggagact cgcgccgcgt gaggacgatc tgcgtgtcgg tgaccgcggc gcgcgcgtgg
      421 tccgccgtca ccgagtgggt tcccgcggcg aacgcggcga gcagcgtctt gtcggcgtag
      481 atgttgatgc ggcgcgtgag cccttccgag gcctcgttga tgatgcgcaa gacctcgggg
      541 ccgaagaggt cgggcccgtg gtatcccgcg gcgcgcagcc ggaaattcag gtagtccttc
      601 acctcgcgcg gcggcagcgg cgcgagcgtg aagccgtggg tgatgcgctc cttcagctgg
      661 cgcatgtgct cgagcgcgag gtgggcgtcg agctcgggct gcccgaagag cacgatctgc
      721 agcagcttct cggtccccgt ctcgaggttc gagaggaggc gaatctcctc gagcgtcgcg
      781 agcggcatcg cgtgcgcctc gtcgatgagc gccacgacct ggcggcccgc cgagtggacg
      841 tcgatcagct tgtcctgcag cgagcggatg aggcgcgtcg tgttggcgcc cgtggtgtcg
      901 atgccgaggt cgttggcgat cgccgcgagc atttcatcgc gcgacaggct cggcaccgcg
      961 aggtagatcg tctccaccgt ctcggggagg cgctccatca gcacgcggca cagcatcgtc
     1021 ttgccgctgc cgacctcgcc cgtcaccttg accatgcctt cgccggcggt gatcgcgtag
     1081 aggagcgctt cgagcgtcgc gccgcggttc gcgcccgaga agaagaactc ggtgtgcggc
     1141 gtgatgcgga acggcgcctc gcgcagtccg aagtgttcga ggtacatcgt catcggcgga
     1201 tttcctggcg cgggcagctg cccttcgggg ccgctctagc cgcgcgcttc gccagggtcg
     1261 ggggaagcca gtttagcacc ggagcgcccc gcggcctcga gccttccata gagcgtgctc
     1321 ctcgaaacgc ccagcatctt cgcggcctgc gacatgttgc cttgggcgat ctcgatcgcg
     1381 gcgtcgaggt acgcctgctc gacgcacttc aacgccgcgt cgaggctgaa gggcttgccg
     1441 gagaggagct ccgcgcgcgc cgccgcgagg ggatccgcgc ggctcgcgtc cgccggcgcg
     1501 aattccgcct cgagatcgcg cgccgccacg gtgtatcccg cgtacttggt ggtgaggcgg
     1561 atcacgatgt tcttcagctc gcgcacgttt cccggaaacg ggtacgacat ccagcgcgcg
     1621 cgcgccgcct cgtcgaacgc gaacgcctcg ctcccggtct tcgccgcgta gagggcgcgg
     1681 tagtgatcga gcaggcgcag cttgtcgtcg cccagctcgc gcagcggcgg gacgcgcagc
     1741 tcgaagaccg acaggcgatg gtagaggtcc gagcgaaagc gcccgtgctt cacctcgtcg
     1801 cgaaggtcgc gattggtggc ggccacgatg cgcgccctcg acacgcgcgt gctcgtctcg
     1861 cccactcgct ggtattcgcc gttctcgagg acgcgcagca gcttcgcctg cagctcgccg
     1921 ggaagctcgc cgatctcgtc gaggaagagc gttccctcgc ccgcgtcctc gaagtagccg
     1981 gacttcgcct gctgcgcccc ggtgaacgcg cccttcgcgt agccgaagag cgtcggctcg
     2041 acgagcgccg gcgagatcgc cgcgcagttg agcgcgagga acggcgcctt cgcgcgggcg
     2101 ctgaggtggt gcagcgagaa ggcgacgcgc tccttgccgc ttcccgattc gccttcgatc
     2161 agtgccggaa acgccgagga cgcgtacagg tcgatctggc tcctcagccg ccgcagcggc
     2221 gggctctcgc cgatcagcgc cttcacgtcc tcggcctcgt cctcgcgccg gtcgagcgcg
     2281 cgcgatttgt aggcccgctc gaggatcgag cgcaggtact cgggctcgca gggcttgggc
     2341 acgagctcca ccgcgccgag ggcgcgcgcg cgccgcgcgt tcgactcctc gttctgcccc
     2401 gtgagcacga gaatgcgcac ctccggcgcg tgcgcgagga gatcgccgat cagcttgaag
     2461 ccttcgttgg gaaggtgcgc cacgggagga agcccgaggt cgaccagcgc gaggtcgggc
     2521 cgcagccccg cgcgcagcgc cgcgatcgcc gccacgcggt ccgccgcgac ggtgacttcg
     2581 tattggccgg cgagcgcgaa ggcgagcgac tccacgatga ggggatcgtc gtcgaccagc
     2641 agcaggcggg gcttcttcac gcgccgatta taggggaggg gcgcggggca aagctgctaa
     2701 aatgcgattc ccctccctcg cgatttcccc gcgtgtccga agaagaaaac ctggtcgatg
     2761 tcgaatcggt gaacttcgcc tatggccggc gcccgatcct caagggaatt tcaatgcgcg
     2821 tgcagcgcgg caaggtggtc gcgatcatgg gcgcgtcggg cagcggcaag acgacgctgc
     2881 tgcgcctcat cggcgggcag ctgaggccct cttccggcac cgtgcgggtc gacggcgcgc
     2941 tcatcagcga gctcgacgac gaggcgctct accgcgagcg ccgcaagatg agcatgctct
     3001 tccagttcgg cgcgctgttc accgacatga gcgtcttcga gaacgtcgcc tttccccttc
     3061 gcgagcacac gcagctcccc gaggagatga tccgcgatct cgtgctgatg aagctcgagg
     3121 cggtcggaat gcgcggcgcg cggcgcctgc gccccgcgga gctctcgggc ggcatggcgc
     3181 ggcgcgtggc gctcgcccgc gcgatggtgc tcgacccccg gctggtcatg tacgacgagc
     3241 ccttcgccgg cctcgacccg atcgcgctcg cggtgatcgg ccagctcatc cgcaagttga
     3301 acgacgcgct cggctcgacc accatcatcg tgacccacga cgtcgtcgag tcgctgcaga
     3361 tcgtcgacta tctctatttc atctccgacg gcgtcatcgt gggggaaggc acgcccgagg
     3421 agatccgccg ctcggagctt ccctacgtga agcagttcgt gcacggcgag atcgacggcc
     3481 ccgtcccgtt ccactacccg gcgcggccct accccgacga tttcgccctt gcaagcggag
     3541 gcgcccgtgc ttgacgtgag aaggcccctc gcccgcctgg gcgcaaacgt gatcaacggc
     3601 atctggcgcg cggggaccat ggcgcgcttc ttcgccctgg tgctcgcctc gtcggcgacg
     3661 agcttcgggc gcttccgcct ggtgatgcgc gaggtctact tcagcggcgt gctctcgctc
     3721 atcatcatca tcgtttcggg gcttttcatc ggcatggtcc tggggctgca gggctaccag
     3781 acgctgcagc gctacggcgc gaccgagtcc atcggggtcc tcgtcgcgct ttcgctggtg
     3841 cgcgagctgg ggccggtagt ctcggcgatc ctcttcgccg cgcgcgccgg ttccgccatc
     3901 accgcggaga tcggcctcat gaagaccacc gagc
//
LOCUS       DASVII010000729         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_105096,
            whole genome shotgun sequence.
ACCESSION   DASVII010000729 DASVII010000000
VERSION     DASVII010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105096"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /gene="greB"
                     /locus_tag="VF309_09505"
     CDS             complement(<1..484)
                     /gene="greB"
                     /locus_tag="VF309_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX7466865.1"
                     /translation="MSKAFTREDDAAPEDDHDVEEDNPIPAGSKNYLTPIGWRRMRDE
                     LTWLVKTERPQVTSVVSWAAKLGDRSENADYQYGKKRLREIDRRIRYLTKRLEAAEVV
                     DPSTREDTDQVFFGATVTYSTPGGQEQTIRIVGIDETDLPKNYVSWISPIARALIKAR
                     E"
     gene            complement(481..1080)
                     /locus_tag="VF309_09510"
     CDS             complement(481..1080)
                     /locus_tag="VF309_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7466866.1"
                     /translation="MKTEFVSAVVLLLLVIDPFGNVPLVNAMLAGVSGARRRLVIVRE
                     CAIAAAILALFMMFGRALLELMHLSETSLSIAGGVILFMIAIRMVFAHPEGAFGPHQG
                     GEPLIVPLAVPLIAGPSALATVMLMATREPGKMGMWAAALATTMVLATLILVTGDRMQ
                     RWAGERPMQAIERLMGLILTVIAVEMLLGGIRAFVEGLK"
     gene            1106..>1933
                     /locus_tag="VF309_09515"
     CDS             1106..>1933
                     /locus_tag="VF309_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7466867.1"
                     /translation="MSRVAFLCLLAGGCAIAFAPIFVRLADTGPVASAFWRCALAVPV
                     LWSWVLLKGERGASVERRVSVMSLVAAGLFFASDLGVWHFSILYTSVANSTVLANLAP
                     IFVTLAGWILWRRKVTRTFLVGMFVAIAGMFVLVGPNFAAGGRPLVGDALGALTAVFY
                     AGYFLAIKIARDAGASTARLMAWSTTITALVLLPIAAAFPQPFWPSGAAGWWVLVGLA
                     LVTQVLGQGLIAYAFAHLPASLSSVSLLIQPVMAALFAWLLFGEAIGAAQYLGGAIVL
                     "
BASE COUNT          266 a          655 c          669 g          343 t
ORIGIN      
        1 cctcgcgcgc cttgatgagc gcgcgcgcga tgggcgagat ccagctcacg tagttcttcg
       61 gcaggtccgt ctcgtcgatg ccgacgatgc gaatggtctg ctcctgccct ccgggagtcg
      121 aatacgtgac cgtcgcgccg aagaagacct ggtcggtgtc ctcgcgcgtg gagggatcga
      181 cgacctcggc ggcctcgagg cgcttggtga ggtagcggat gcgccggtcg atctcgcgca
      241 ggcgcttctt gccgtactgg tagtcggcgt tctcggagcg gtcgccgagc ttcgcggccc
      301 acgagacgac ggaagtcacc tgcgggcgct cggtcttcac gagccacgtg agctcgtcgc
      361 gcatgcgccg ccacccgatc ggggtgaggt agttcttcga gcccgccggg atcggattgt
      421 cttcctcgac gtcgtgatcg tcctcgggcg cggcatcgtc ttcgcgcgtg aacgccttgc
      481 tcacttcaag ccctccacga acgcccggat cccgccgagc agcatctcca ccgcgatcac
      541 ggtgaggatg aggcccatga ggcgctcgat cgcctgcatc gggcgctcgc ccgcccagcg
      601 ctgcatccgg tcacccgtca cgaggatcaa ggtcgccagc accattgtcg tggcgagggc
      661 cgcggcccac attcccatct tcccgggctc gcgcgtggcc atgagcatca cggtggcgag
      721 ggccgaagga cccgcgatga gcgggaccgc gagcgggacg atgaggggct cgccgccctg
      781 gtgcgggccg aaggcgccct cgggatgggc gaagaccatg cggatcgcga tcatgaaaag
      841 gataacaccc cccgctatgg acaacgaagt ctcggacaag tgcatgagct cgaggagcgc
      901 gcgcccgaac atcatgaaga gggcgaggat cgccgcggcg atcgcgcact cgcgcacgat
      961 caccaggcgc cggcgcgcgc ccgaaacgcc ggcgagcatc gcgttcacca ggggcacgtt
     1021 gccgaaggga tcgatcacca gaaggagcag gaccaccgcc gagacgaatt ctgtcttcat
     1081 atggagcgtt atcatattcg cttccatgag ccgggtagcc ttcctctgcc tcctcgccgg
     1141 gggctgcgcg atcgcgttcg cgccgatttt cgtgcggctt gccgacacgg ggccggtggc
     1201 gagcgccttc tggcgctgcg cgctcgcggt gccggtgctg tggagctggg tgctgctgaa
     1261 aggcgaacgc ggcgcaagcg tcgagcgccg cgtgtcggtg atgtcgctcg tcgcggccgg
     1321 gctcttcttc gcgagcgatc tcggcgtctg gcacttctcg atcctctaca cgtcggtcgc
     1381 gaattccacg gtgctcgcca acctcgcgcc catcttcgtc acgctggcgg gatggatcct
     1441 ctggcgccgc aaggtgacgc gcaccttcct cgtcggcatg ttcgtggcga tcgcgggaat
     1501 gttcgtgctg gtggggccga acttcgccgc cggcggcagg cctctcgtgg gcgacgccct
     1561 cggcgcgctg accgcggtct tctacgccgg ttacttcctc gcgatcaaga tcgcgcgcga
     1621 cgcgggcgcg tcgacggcgc gcctcatggc gtggagcacg acgatcacgg cgctcgtgct
     1681 gctgccgatc gccgcggcct tcccgcagcc cttctggccg tcgggcgcgg cgggatggtg
     1741 ggtgctcgtc gggctcgcgc tggtgacgca ggtgctcggc cagggcctca tcgcgtacgc
     1801 gttcgcgcac ctgcccgcgt cgctctcctc ggtctcgctg ctgatccagc cggtgatggc
     1861 ggcgctcttc gcgtggctcc tcttcggcga ggcgatcggc gccgcgcaat atctcggcgg
     1921 cgcgatcgtg ctc
//
LOCUS       DASVII010000730         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_717511,
            whole genome shotgun sequence.
ACCESSION   DASVII010000730 DASVII010000000
VERSION     DASVII010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717511"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VF309_09520"
     CDS             <1..536
                     /locus_tag="VF309_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX7466868.1"
                     /translation="KKLLAIALLMSSTIASGATCSALLDHKVTSLLGDKEDLCQYAGK
                     VVLVVNTASYCGYTPQYKGLQALNEKYKPRGLVILGFPSNDFGEQEPGSAKEIADFCD
                     RTYAVKFPMFSKTSVKEPGGSAVFDGLAKATGQRPKWNFHKYLIGRDGTTVASFPSKV
                     EPESPEFVAKVEQLLGS"
     gene            complement(547..1680)
                     /locus_tag="VF309_09525"
     CDS             complement(547..1680)
                     /locus_tag="VF309_09525"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX7466869.1"
                     /translation="MRPLTITAIAALALAIALAFGARVGHPASALPEKVFALSLERLG
                     PTPRDPSNRWADDVAAADLGRRLFFDQRLSGNGRVSCATCHDPENDFQDAMPLAKGMG
                     TTNRRTMPIAGAAYGRFFFWDGRKDSLWSQALGPLESPVEHGGDRMRYAEIVAAHYRE
                     GYQRVFGPLPEPGEREGATRIFVNIGKAIEAYERRIQFRPSRFDRFVREWRKSGVMPR
                     DILDEREIAGLKLFIGKAACVDCHKGPLLNHDKFFNTGVPVRGALPPDHGRATAIASL
                     KADEFNCRSRWSDASPEECRELPRRVPASERAFKVPSLRNVADRAPYMHAGQFATLAE
                     VLDHYNRAPGESVLKALHLKPAELGDLEAFLRTLSGGVAAPGT"
     gene            complement(1687..>2326)
                     /locus_tag="VF309_09530"
     CDS             complement(1687..>2326)
                     /locus_tag="VF309_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466870.1"
                     /translation="ESHVPFAINVPAEVIRGNLASPEKLAQYLGNAGVDMNEEAVIIS
                     KGGLNPDSALAYLALEQLGQKKVSLLSDSLDDWAFAGLTVEKSGEPVDPKKPPSPPAK
                     PKAYRADVRTGIVTRNPSATKGLYPKVYVATGRSLPARVPEGKFVHVPYTELLNADGT
                     PKPAKDIWAALQKAGVPRYAEIVSFSDDPGEAAADYFVLKLMGYPDVKVWVM"
BASE COUNT          380 a          778 c          781 g          387 t
ORIGIN      
        1 tgaaaaaact cctcgccatc gccctcctca tgtcctccac catcgcatcc ggcgcgacct
       61 gcagtgccct tctcgaccac aaggtgacga gcctcctcgg cgacaaggag gatctttgcc
      121 agtacgcggg caaggtcgtc ctcgtggtga acaccgcgag ctactgcggc tacacgccgc
      181 aatacaaggg cctgcaggcg ctgaacgaga agtacaagcc tcgcggtctc gtgatcctcg
      241 gatttccctc gaacgacttc ggcgagcagg agcccggcag cgcgaaggag atcgcggact
      301 tctgcgatcg aacctacgcg gtcaagttcc cgatgttctc gaagacgtcg gtgaaggagc
      361 cgggaggctc ggcggtgttc gacgggctcg cgaaggcgac cgggcagcgc ccgaagtgga
      421 atttccacaa gtacctgatc gggcgcgacg gcacgactgt ggcgagcttt ccctcgaagg
      481 tcgagccgga gtcgccggag ttcgtcgcca aagtcgagca gctcctgggc agctgaggcg
      541 cacgcctcag gtccccggcg ccgcgactcc accgctcagc gtgcgcagga acgcctcgag
      601 atcacccagc tccgcgggct tgagatgcag cgccttcagc accgactcgc cgggcgcgcg
      661 attgtagtga tccaggacct cggcgagcgt ggcgaactgt cccgcatgca tgtagggcgc
      721 gcgatccgcc acgttgcgaa gcgacggcac cttgaaggcc cgctcgctcg cgggaaccct
      781 gcggggaagc tcgcggcatt cctcggggct ggcgtcgctc cagcggctgc ggcaattgaa
      841 ctcgtcggcc ttcagtgaag cgatcgcggt cgcgcgccca tgatccggcg gcagcgcgcc
      901 ccgcacgggg acgccggtgt tgaagaactt gtcgtggttg aggagcggcc ccttgtggca
      961 atcgacgcaa gccgccttgc cgatgaagag cttcagtccg gcgatctccc gctcgtcgag
     1021 gatatctcgc ggcatgaccc cggacttgcg ccactcgcgc acgaagcgat cgaagcgcga
     1081 gggccggaat tggatgcggc gctcgtacgc ctcgatggcc ttgccgatgt tcacgaagat
     1141 gcgcgtcgcc ccttcgcgct cgccgggctc cggcagcgga ccgaacacgc gctggtaccc
     1201 ttcgcggtaa tgcgccgcga cgatttccgc gtaccgcatg cgatcgccgc cgtgctcgac
     1261 cgggctttcg agcggcccca gcgcctgcga ccagaggctg tccttgcggc cgtcccagaa
     1321 gaagaatcgg ccgtatgcgg cgccggcgat gggcatcgtg cggcgattgg tcgtgcccat
     1381 gcccttggcg agcggcatcg cgtcctggaa gtcgttctcg ggatcgtggc acgtcgcgca
     1441 cgacacgcgc ccgtttccgc tcaggcgctg gtcgaagaag agccggcgcc cgaggtccgc
     1501 ggccgccacg tcatcggccc agcggttgct gggatcgcga ggcgtcggcc cgagcctttc
     1561 gagcgacaag gcgaacacct tctcgggaag cgccgacgcc gggtggccga ccctcgctcc
     1621 gaaggccaac gcaatggcga gcgccagggc ggcgatcgcg gtgatcgtga gcggtctcat
     1681 gggttcctac attacccaga ctttcacgtc cgggtaaccc atcagcttga gcacgaagta
     1741 gtccgccgcc gcctcgccgg gatcgtcgga gaagctcacg atctccgcat agcgcggcac
     1801 gccggccttc tgcagcgcgg cccagatgtc tttagcgggc ttgggcgtgc cgtccgcgtt
     1861 caatagctcg gtgtagggga cgtgcacgaa cttgccctcg ggcacgcgcg cgggaagcga
     1921 cctgccggtg gcgacgtaga ccttcggata gaggcccttc gtcgcggacg ggttgcgcgt
     1981 gacgatcccg gtgcggacgt ccgcgcgata cgccttcggc ttcgcgggcg gggacggcgg
     2041 cttcttcgga tcgacgggct cgccgctctt ctccaccgtg aggcccgcga acgcccagtc
     2101 atccagcgaa tcggagagca gcgagacctt cttctggccg agctgctcga gcgccaggta
     2161 cgcgagcgcg gaatcgggat tgaggccgcc cttcgagatg atgacggcct cctcgttcat
     2221 gtccacgccg gcgttgccca ggtattgcgc gagcttctcc ggcgacgcga gattgccgcg
     2281 aatcacttcg gccggaacgt tgatcgcgaa tggcacgtgc gactcg
//
LOCUS       DASVII010000731         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_609493,
            whole genome shotgun sequence.
ACCESSION   DASVII010000731 DASVII010000000
VERSION     DASVII010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609493"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VF309_09535"
     CDS             <1..1494
                     /locus_tag="VF309_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466871.1"
                     /translation="PESHVLYGGRDLLKASLAQLQAIRGKDISVIFQEPMTSLNPVFT
                     VGEQIAEVLRLHMGMSLRQAAARAIELLGEVGIPNPQLRVNSYPHEMSGGQQQRVMIA
                     MAIACEPKLLIADEPTTALDVTIQKQILDLVASLQVKHNMSVLFITHDLGVVGDIADS
                     VVVMQAGEIKEQGPVKAIFEQPQHPYTKALLACRPRLDARPRRLPVIDDFMKGEGRVH
                     VGDRARGTSPGDPIILEVKGLTKTFFLKEGLFGKRAVPAVRNVSFSLPRGKTLGLVGE
                     SGSGKTTVGLTLMRLHDATSGEVLFEGKDLLALSEREMMAYRRRIQIIFQNPYASLNP
                     RMTVGHILVEPMKIHSIGSGERERVDMAFQLLKRVGLSEQSFYKYPHEFSGGQRQRIA
                     IARCLTMKPDVLICDESVSALDVSVQAQVLNLLQDLQDEYGLSYIFISHDLAVVKYIS
                     DKVMVMNEGAIIEMADSDEIYRHPREEYTRKLLSSIPKGYGLAAQPS"
BASE COUNT          273 a          510 c          502 g          255 t
ORIGIN      
        1 cccgagagcc acgtgctcta cggcgggcgc gatcttctca aggcgtccct ggcgcagctg
       61 caggcgattc gcggcaagga catctcggtg atcttccagg agccgatgac gtcgctgaat
      121 cccgtgttca ccgtgggcga gcagatcgcg gaggtgctgc gcctgcacat gggcatgagc
      181 ctgcgccagg cggcggcgcg cgcgatcgag ctcctgggcg aggtcggcat cccgaacccg
      241 cagctgcgcg tgaattccta tccccacgag atgtccggcg ggcagcagca gcgcgtgatg
      301 atcgcgatgg cgatcgcctg cgagccgaag ctcctcatcg ccgacgagcc gaccaccgcg
      361 ctcgacgtca ccatccagaa gcagatcctc gacctcgtcg cgagcctgca ggtgaagcac
      421 aacatgtcgg tgctcttcat cacccacgac ctcggcgtcg tgggcgacat cgccgacagc
      481 gtggtcgtca tgcaggcggg cgagatcaag gagcagggcc cggtcaaggc gatcttcgag
      541 cagccgcagc atccctacac caaggcgctc ctcgcctgcc ggccgcggct cgacgcgagg
      601 cccaggcgat tgccggtgat cgacgatttc atgaagggcg agggccgcgt ccacgtcggc
      661 gatcgcgcgc gcggcacgtc gcccggcgat ccgatcatcc tcgaggtgaa ggggctcacc
      721 aagacgttct tcctgaagga agggctcttc ggcaagcggg cggtccccgc ggtgaggaac
      781 gtgagcttct cgttgccccg gggaaagacg ctcgggctgg tcggcgagtc gggctccggc
      841 aagacgacgg tcggcctcac gctgatgcgg ctgcacgacg cgaccagcgg ggaggtgctc
      901 ttcgaaggca aggacctcct cgcgttgagc gagcgcgaga tgatggccta ccgccgccgc
      961 atccagatca tcttccagaa tccgtacgcc tcgctcaacc cgcgcatgac ggtcgggcac
     1021 atcctcgtcg agccgatgaa gatccactcc atcggcagcg gcgagcgcga gcgcgtggac
     1081 atggcgttcc agctcctgaa gcgcgtgggg ctgtcggagc agtcgttcta caagtacccc
     1141 cacgagttct cgggcgggca gcgccagagg atcgccatcg cgcgctgcct cacgatgaag
     1201 cccgacgtgc tgatctgcga cgagtcggtc tccgcgctcg acgtgtcggt ccaggcgcag
     1261 gtgctgaacc tgctgcagga cctgcaggac gagtacgggc tttcgtacat cttcatctcc
     1321 catgacctcg cggtggtgaa gtacatttcc gacaaggtga tggtgatgaa cgagggcgcg
     1381 atcatcgaga tggccgattc cgacgagatc taccgccatc cgcgcgagga atacacgcga
     1441 aagctcctat cctcgatccc caagggctac gggctcgccg cccaaccttc ctgattcacg
     1501 gagtccgtcg atgcatcgcc ttgttgcctt catcgcggcc
//
LOCUS       DASVII010000732         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_70444, whole
            genome shotgun sequence.
ACCESSION   DASVII010000732 DASVII010000000
VERSION     DASVII010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70444"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..>1613)
                     /gene="kdpB"
                     /locus_tag="VF309_09540"
     CDS             complement(102..>1613)
                     /gene="kdpB"
                     /locus_tag="VF309_09540"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX7466872.1"
                     /translation="WLVVRVSVNPGEAFLDRMIAMVEGARRQKTPNEIALWILLVGFT
                     LVFLLATATLLPYSIYAVEVTKAGSAVTVTVLVALLVCLIPTTIGGLLSAIGLAGIGR
                     TLRANVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQASDFLPGSGVRREDLADAAQL
                     ASLADETPEGRSIVILAKQKYNIRERDVATLGATFVHFTAHTRMSGVDLGERHIRKGA
                     ADAVKAFVESQGGRFLPEMQAIVDMVARRGSTPLVVADGPRVMGVVELKDIVKGGIKE
                     RFAQLRRMGIKTIMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLELIRKEQAGGRLV
                     AMTGDGTNDAPALAQADVALAMNSGTQAAKEAGNMVDLDSNPTKLIEVVEIGKQLLMT
                     RGSLTTFSIANDVAKYFAIIPAAFATTYPQLNTLNVMYLATPNSAILSAVIFNALVII
                     ALVPLALKGVRYTPMGAAAVLRKNLWIYGAGGVIVPFIGIKAIDMILVVLGLA"
BASE COUNT          252 a          537 c          540 g          284 t
ORIGIN      
        1 cccgtcacgg cggtgagggc tacgagcagg accagggcag ggcgcagatt ttctttcatg
       61 ttcatgttca tgttcgattc ctcatgttca cgttcgatgc ctcaggcgag tcccagcacc
      121 acgaggatca tgtcgatcgc cttgatgccg atgaagggca cgatcacgcc gcccgcgccg
      181 tagatccaga ggttcttgcg cagcaccgct gcggcaccca tcggcgtgta cctcacgccc
      241 ttgagcgcga gcggcaccag cgcgatgatg acgagggcgt tgaagatcac cgccgacagg
      301 atcgcgctat tgggcgtggc gaggtacatc acgttcaacg tgttcagctg cgggtaggtc
      361 gtcgcgaacg ccgccgggat gatcgcgaag tacttcgcga cgtcgttggc gatcgagaac
      421 gtggtgagcg agccgcgcgt catgaggagc tgcttgccga tctcgaccac ctcgatgagc
      481 ttcgtgggat tggagtcgag gtcgaccatg ttgccggcct ctttcgccgc ctgcgtgccg
      541 ctgttcatcg cgagcgccac gtcggcctgg gcgagggcag gggcgtcgtt ggtgccgtcg
      601 ccggtcatcg cgacgaggcg gcctcccgcc tgctccttgc ggatgagctc gagcttcgct
      661 tccggagtcg cctcggcgag gaagtcgtcc acgcccgcct cggcggcgat cgcggccgcc
      721 gtgagccggt tgtcgccggt gatcatgatg gtctttatgc ccatgcggcg aagctgcgcg
      781 aagcgctcct tgatgccgcc cttcacgatg tccttcagct cgaccacgcc catcacgcgc
      841 gggccgtccg ccaccacgag cggggtgctg ccgcggcgcg cgaccatatc gacgatcgcc
      901 tgcatctccg ggagaaatct cccgccctgg gattcgacga acgccttgac cgcgtcggcc
      961 gcgcccttgc ggatgtgccg ctcgcccagg tccactccgc tcatgcgcgt atgcgccgtg
     1021 aagtggacga aggtcgcgcc cagcgtcgcg acgtcgcgct cgcggatgtt gtacttctgc
     1081 ttggcgagga tgacgatgct gcgcccctcg ggcgtctcgt ccgcgagcga ggcaagctgc
     1141 gccgcgtcgg cgagatcctc gcgcctcacg ccgcttccag gaaggaaatc gctcgcctgg
     1201 cggttgccca gcgtgatcgt gccggtcttg tcgaggagga gcacgtcgac gtcgccagcc
     1261 gcttccacgg cgcggcccga catcgcgatc acgttggcgc gcaaggtcct gccgatgccg
     1321 gcaaggccga tggcggagag cagcccgccg atggtcgtcg gaatgaggca cacgaggaga
     1381 gccaccagca cggtcaccgt gacggcgctg ccggccttcg tgacctctac agcgtagatg
     1441 gagtacggca gcagcgtcgc ggtggcgagc aggaacacga gcgtgaatcc caccagcagg
     1501 atccacagcg cgatctcgtt gggcgtcttc tggcgccgcg cgccctcgac catggcgatc
     1561 atgcggtcga ggaaggcctc gccgggattc acgctcacgc gcaccaccag cca
//
LOCUS       DASVII010000733         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_70472, whole
            genome shotgun sequence.
ACCESSION   DASVII010000733 DASVII010000000
VERSION     DASVII010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70472"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..821)
                     /locus_tag="VF309_09545"
     CDS             complement(120..821)
                     /locus_tag="VF309_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7466873.1"
                     /translation="MKALEHVELVTGAEPRGTVIWMHGLGADGWDFVPIVRELPLPSD
                     LALRFIFPHAPERAVTINDGYRMRAWYDISQSDLARVPDEKGIRESQAQIEELIARER
                     GRGVEASRIVLAGFSQGGAIALQAGLRHPARLGAIVALSTYLPLEDSLDREASAANES
                     TPIFMAHGTQDPVVPLGLAERSREALRMRGYDVQWHAYPMPHSVSAEEVQDLASFLAG
                     VFGAAPDKRSSILLP"
     gene            complement(818..1423)
                     /gene="mog"
                     /locus_tag="VF309_09550"
     CDS             complement(818..1423)
                     /gene="mog"
                     /locus_tag="VF309_09550"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX7466874.1"
                     /translation="MSEAPDAVRIGLVAASDRASQGVYKDEGIPALEQWLAAALANPI
                     EFVPRLIPDERARIEATLKELVDERACDLVVTTGGTGPAARDVTPEATLAVADREMPG
                     FGEQMRRISLEFVPTAILSRQVAVIRGKCLILNLPGQPKSIRETLEGLKSPDGKPLVA
                     GIFAAVPYCIDLIGGPYLETHDAIVKAFRPKSAIRPPRSAP"
     gene            complement(1420..>1760)
                     /gene="yjgA"
                     /locus_tag="VF309_09555"
     CDS             complement(1420..>1760)
                     /gene="yjgA"
                     /locus_tag="VF309_09555"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX7466875.1"
                     /translation="HEARRRQVQFIGKLMRNVDASPIAERLRALEAPTQRDTALLHLA
                     EKWRQELIGDPGAMARFKQEFPEADQDHLANLVKGALEERRTSRPPKFFRELFHAVNT
                     LVQDHARRNS"
BASE COUNT          262 a          596 c          626 g          276 t
ORIGIN      
        1 agccccgtga tcgggctcgt ggtcgagacg ggctgcaact acttcacgac gctcacctac
       61 ccggagccgg tgacggcggc gctgcgcctc gccctcgagc ccctcgcgtc gcccgcaccc
      121 tagggcagca ggatcgagga gcgcttgtcg ggcgcggcgc cgaagacgcc cgcgaggaat
      181 gaagcgagat cctgcacttc ctcggcgctc acggagtgcg gcatcggata cgcgtgccat
      241 tgcacgtcat agccgcgcat gcgcaacgcc tcccgggacc tctcggcgag ccccagcggg
      301 accacggggt cctgcgttcc gtgcgccatg aagatcggcg tggactcgtt ggccgcggag
      361 gcctcgcgat cgagcgagtc ttcgagcggc aggtaagtcg agagcgccac gatcgcgccg
      421 aggcgcgccg ggtgcctcag gcccgcctgc agggcgatgg cgccgccttg cgagaatccc
      481 gcgagcacga tgcgcgaagc ctccacgccc cggccgcgct cgcgtgcgat gagctcttct
      541 atctgcgctt gcgactcgcg gatgcccttc tcgtcgggga cgcgcgcgag gtcgctctgc
      601 gagatgtcgt accaggcgcg catgcggtag ccgtcgttga tggtgacggc acgctcgggc
      661 gcgtgcggaa agatgaatcg caacgcgaga tcggagggaa gcggcagctc cctcacgatg
      721 ggcacgaagt cccagccatc ggcgccgagg ccgtgcatcc agatgacggt gccgcgcggc
      781 tccgcgccgg tgacgagctc gacgtgctcc agcgccttca tggcgcgctc cttggcggac
      841 ggatcgcgga cttcggacgg aaggccttca cgatcgcgtc gtgggtttcg agatagggac
      901 cgccgatgag gtcgatgcag tagggcacgg cggcgaagat tcccgcgacg aggggcttgc
      961 cgtccggcga cttcaatccc tcgagcgtct cgcggatcga cttcggctgg cccgggagat
     1021 tcaggatgag gcacttgccg cgaatcaccg cgacctgccg cgagaggatc gcggtgggaa
     1081 cgaattcgag gctgatgcgg cgcatctgct cgccaaagcc cggcatctcg cgatcggcca
     1141 ccgcgagcgt cgcctcggga gtgacgtcgc gcgccgcggg gccggtgccg cccgtggtga
     1201 cgacgagatc gcaggcgcgc tcgtcgacga gctccttcaa cgtggcctcg atgcgcgcgc
     1261 gctcgtcggg aatgagccgc ggcacgaact cgatcgggtt cgcgagcgcc gcggcgagcc
     1321 attgctcgag ggccggaatg ccctcgtcct tgtagacgcc ttgcgacgcg cggtcgctcg
     1381 ccgccacgag gccgatgcgg accgcgtccg gtgcttcgct catgaattcc tcctcgcatg
     1441 atcctggacc agcgtgttca ccgcgtggaa gagctcgcga aagaacttcg gcggccgcga
     1501 cgtgcgccgc tcctcgaggg cgcccttcac gagattcgcc aggtggtcct ggtcggcctc
     1561 gggaaactcc tgcttgaacc gcgccatggc cccggggtcg ccgatgagct cctggcgcca
     1621 tttctccgcg aggtggagga gcgccgtgtc gcgctgcgtg ggagcctcga gggcgcgaag
     1681 gcgctcggcg atcggcgacg catcgacgtt gcgcatgagc ttgccgatga actgcacctg
     1741 ccggcgccgc gcctcgtgct
//
LOCUS       DASVII010000734         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_177629,
            whole genome shotgun sequence.
ACCESSION   DASVII010000734 DASVII010000000
VERSION     DASVII010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177629"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VF309_09560"
     CDS             complement(<1..529)
                     /locus_tag="VF309_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7466876.1"
                     /translation="MRILVLNWQDSENPQAGGAELHLREVFSRIVARGHTVDLLCSSW
                     PNAPQRVNLAGIDVHRIGTRHTYPFLAHRYYNENLARNNYDVVIEDLNKVPLYTPLWD
                     VRKLVALVHHLFGATVFREANAPLAVAVWLSERPLGTLYRRVPFEAVSVSTAEDLMDR
                     GIPRSSIRVIYNGVDA"
     gene            complement(529..939)
                     /gene="nusB"
                     /locus_tag="VF309_09565"
     CDS             complement(529..939)
                     /gene="nusB"
                     /locus_tag="VF309_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7466877.1"
                     /translation="MRIETRARARALQALYACEMRGGKNLDRVATQVWDDLAVAPEER
                     RLAGLLVREVASAGTALDTRLAELTTNWRLERIGAIERSVLRLGAAELMKGETPPRVA
                     IQEAVRLAERFGTSASAKFVNGVLDAFARQAGMI"
     gene            complement(929..1408)
                     /gene="ribH"
                     /locus_tag="VF309_09570"
     CDS             complement(929..1408)
                     /gene="ribH"
                     /locus_tag="VF309_09570"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7466878.1"
                     /translation="MAEFHGLPSGVGRRVVVLASRFNEPIVKKLVDGALEALIKYDVA
                     FEDIDVVWVPGAWELPIAARVLLATERYDGLVAVGAVIRGETAHFDYIAGEASRGLAA
                     VSADSEIPIGFGVLTCDTDEQAEARAGGAHGNKGWDAAVATLEMIDLLGRLAPDDAD"
BASE COUNT          275 a          505 c          509 g          279 t
ORIGIN      
        1 atgcgtcgac accgttgtag atcacacgaa tggagctgcg tggaattccc cgatccatga
       61 ggtcctcagc cgtgctcacg ctgaccgcct cgaaggggac acggcgataa agcgttccga
      121 gcggacgctc ggagagccac actgccacgg caagtggtgc gttggcctcg cggaataccg
      181 tcgcgccgaa gaggtggtgc accagcgcga cgagcttgcg cacatcccag agcggcgtgt
      241 agagcgggac cttgttcaga tcctcgatga ctacatcgta gttgttgcga gcgagattct
      301 cgttgtagta gcgatgggcg agaaatggat acgtgtggcg cgtgccgatt cgatgaacgt
      361 cgatcccggc cagattcacg cgctggggcg cgttcggcca cgagctacag agcaggtcga
      421 cggtgtgtcc gcgcgcgacg atacggctga atacttcccg gaggtgaagc tcggcaccgc
      481 cggcctgcgg attctcggaa tcctgccagt tgaggacgag tatccgcact agatcatgcc
      541 ggcctgtcgc gcgaaggcgt cgagcacacc gttgacgaac ttggcgctcg cggatgttcc
      601 gaacctctcc gccagcctca cggcttcctg aattgcgacg cgcggaggcg tctcgccctt
      661 catcagctcg gcggcgccaa ggcggagcac ggatctctcg atggcgccga tccgctcgag
      721 acgccagttg gtcgtgagct cggcgagacg cgtgtccagc gcggtgccgg cgctagcgac
      781 ttcgcgcaca agaagaccgg ccagtcgccg ctcctcgggc gcaaccgcga gatcgtccca
      841 cacctgcgtc gccacccgat caagattctt gccgccccgc atctcgcacg cgtacagcgc
      901 ctgaagcgcc cgcgcgcgcg cgcgtgtttc aatccgcatc gtcgggggcc aggcggccga
      961 gcagatcgat catctcgagc gtcgcgaccg ccgcgtccca tcccttgttg ccgtgcgcgc
     1021 cccctgcgcg cgcctcagct tgctcgtcgg tgtcgcacgt gagcacacca aatccgatgg
     1081 gtatctcgga atcggcgctt acggcggcaa gtccgcggga cgcttcgcca gcgatgtagt
     1141 caaagtgggc ggtctcgccg cggattaccg ctccgaccgc cacgaggccg tcgtagcgct
     1201 cggtggcgag tagcacgcgg gcggcaatgg ggagctccca ggcgcctgga acccacacca
     1261 catcgatgtc ctcgaacgcg acgtcgtatt tgatcagcgc ctcgagagcg ccgtcgacga
     1321 gcttctttac gattggctca ttgaaacggc tggcgaggac gacgacgcgc cgcccaacgc
     1381 cgctcggcaa tccgtgaaac tcggccatct acgaagcgaa gaggtgtccg agcttcgccc
     1441 gtttggcttc gaggtagctt gagttttcgt cgtgcgcgat cgagatgatc ggcacacgct
     1501 cgtcgaccgt cagcccgtaa ccttcgaggc cgacgagctt gcgcggattg ttggtgatgg
     1561 ggcggatg
//
LOCUS       DASVII010000735         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_465702,
            whole genome shotgun sequence.
ACCESSION   DASVII010000735 DASVII010000000
VERSION     DASVII010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465702"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..738
                     /locus_tag="VF309_09575"
     CDS             94..738
                     /locus_tag="VF309_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466879.1"
                     /translation="MNSKTDPAAEPMVARDATYHRAPDALRERLRAGLAREARATDRS
                     RPWSFLAMAASFAAVALVTWNLALLGGGPGADELMARDLVNAHVRSLMIEGHLNDVVS
                     SDQHTVKPWFAGKLDFAPPVVDLANAGFALAGGRLDYVNARAVAVLTYRYRQHVVNVF
                     IWPEAGEVEPRASSRQGFSVVQWTHGGMRYSAVSDAAAAQLFALAEALRAQGAG"
     gene            complement(743..1354)
                     /locus_tag="VF309_09580"
     CDS             complement(743..1354)
                     /locus_tag="VF309_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX7466880.1"
                     /translation="MTQAATPIDFYFDFSSPYGYIASQIVDEMERRIGRPVRWRPTLL
                     GVTFKIMGQVPLVEVPMKGDYARNDFARSARLHKVEYRQPGKFPVGTVAACRAFYWAD
                     ERDPASARALAKALYRAYFTEDKDIGAPVTVVDIARSVGLDATALAAALEDPALKDRA
                     KREVESAIAAGVFGSPFFVVDGEAFWGVDRMPMVEEWIRTGGW"
     gene            complement(1351..1791)
                     /locus_tag="VF309_09585"
     CDS             complement(1351..1791)
                     /locus_tag="VF309_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466881.1"
                     /translation="MPERHAELEDAPMYDPRHDEESAPQPEPVLERTQELKPVHEPKL
                     AYSSAAPPPRAFQAVSEHDAAGEESHRPQRKRREAAHAEAQPQDLQLVETQAEAAPIS
                     VEDDAPRRSKPRRRRSGAAEAEPLMLVETQGNADTQRTDGTPTP"
     gene            1777..2022
                     /locus_tag="VF309_09590"
     CDS             1777..2022
                     /locus_tag="VF309_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466882.1"
                     /translation="MPFGHDVAGLALGSRIACALEPRCAALDRVGGRCALDGEVLARC
                     AFTALTAFATAPTTATATARLAFAILGGARRPILRGL"
     gene            complement(2028..>2211)
                     /locus_tag="VF309_09595"
     CDS             complement(2028..>2211)
                     /locus_tag="VF309_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466883.1"
                     /translation="RAAKRVPRFVAKAAAMRMAKDVEIAVKAVVIAAKAGNSRIARRA
                     RHPCLASKASRKDLSP"
BASE COUNT          277 a          772 c          798 g          364 t
ORIGIN      
        1 cattccgatc ggcacggtga tgtcgcgcct cgcgcgcggg cgcgagctcc tgcgccgcgt
       61 gatcgaggtc cgcatgagaa aggcgtcctg agaatgaatt cgaaaaccga tcccgcggcc
      121 gagccgatgg tggcgcgcga cgcgacctat caccgcgcgc ccgacgcgct tcgcgaaagg
      181 ctgcgagccg gcctcgcgcg cgaggcgcgc gccacggacc gctcgcggcc gtggagcttc
      241 ctcgcgatgg cggcctcctt cgcagcggtc gcgctggtca cgtggaatct cgcgctgctg
      301 ggcggcgggc cgggcgcgga cgagctcatg gcgcgcgacc tggtgaacgc gcacgtgcgc
      361 tcgctcatga tcgaggggca tttgaacgac gtggtgtcgt cggaccagca cacggtgaag
      421 ccgtggttcg ccggaaagct cgacttcgcg ccccccgtgg tcgacctggc gaacgcgggc
      481 ttcgcgctgg cgggcggcag gctcgattac gtgaacgccc gcgccgtcgc ggtgctcacc
      541 tatcgctatc ggcagcacgt cgtgaacgtg ttcatctggc ccgaggcggg cgaggtggag
      601 ccccgcgcga gctcgcggca gggattctcg gtagtgcagt ggacgcacgg cgggatgagg
      661 tacagcgccg tttccgatgc cgcggcggcg cagctcttcg cgctggccga agccttgcgc
      721 gcgcagggcg cgggctaggg cgctaccagc cgccggtccg gatccactcc tcgaccatcg
      781 gcatgcggtc gacgccccag aacgcttcgc cgtcgaccac gaagaagggc gagccgaaga
      841 ctccggcggc gatcgccgac tcgacttcgc gcttcgcgcg atccttcagc gccgggtcct
      901 cgagggccgc ggcgagggcg gtggcatcga gcccgacgga gcgggcgatg tcgaccaccg
      961 tgacgggcgc gccgatatcc ttgtcctcgg tgaaatacgc gcgatagagc gccttggcga
     1021 gagcacgcgc gctcgcggga tcgcgttcat cggcccagta gaacgcgcgg cacgcggcga
     1081 ctgtcccgac gggaaacttc ccgggctgcc ggtattcgac cttgtgcagc cgcgccgagc
     1141 gcgcgaaatc gttcctcgcg tagtcgccct tcatcgggac ctcgacgagc ggaacctgcc
     1201 ccatgatctt gaaggtcacg cccagcagcg tcggccgcca cctcacgggg cggccgatgc
     1261 gcctttccat ctcgtccacg atctggctcg cgatgtagcc gtagggcgag gagaagtcga
     1321 agtagaagtc gatcggcgtt gccgcctgcg tcatggcgtc ggggttccgt ccgtgcgctg
     1381 agtgtcggcg ttgccttgcg tctcgaccag catcaggggc tcggcctcgg ccgctcccga
     1441 gcggcggcgg cgcggcttgc tgcggcgcgg agcgtcgtcc tcgacggaga tcggcgctgc
     1501 ctcggcctgg gtctcgacca gctgaaggtc ctgcggctgg gcctcggcgt gcgcggcctc
     1561 gcgtcgcttg cgttgcggac gatgggattc ctccccggcc gcgtcgtgct cgctcaccgc
     1621 ctggaaggcg cgcggcggtg gcgccgcgct cgagtaggcg agcttcggct cgtgcacggg
     1681 cttcagctcc tgcgtccgtt caaggaccgg ctcgggctgc ggcgcgctct cttcgtcgtg
     1741 gcgcgggtcg tacatgggcg cgtcttcgag ctcggcatgc cgttcgggca tgacgttgcc
     1801 gggctcgcgc tggggagccg catcgcgtgc gctctcgagc cgcgctgcgc ggctctcgat
     1861 cgcgtcggcg ggcgatgcgc cctcgacggc gaagttctcg cgcggtgcgc gttcaccgcg
     1921 ctcaccgcgt tcgccaccgc gcccaccacg gcgacggcga cggcgcgtct ggccttcgcc
     1981 attctcgggg gcgccaggcg tcccatcctg cgggggctgt gaatcgttca cggcgacaag
     2041 tccttgcggc tcgccttcga cgcgaggcac gggtgccggg cgcggcgggc gattctgctg
     2101 ttgccggcct tcgccgcgat caccacggcc ttcaccgcga tctccacgtc cttcgccatg
     2161 cgcatcgccg cggctttcgc cacgaatctc ggcacgcgtt tcgccgcgcg g
//
LOCUS       DASVII010000736         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_35208, whole
            genome shotgun sequence.
ACCESSION   DASVII010000736 DASVII010000000
VERSION     DASVII010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35208"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /gene="pal"
                     /locus_tag="VF309_09600"
     CDS             <1..565
                     /gene="pal"
                     /locus_tag="VF309_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX7466884.1"
                     /translation="CVHRYKETRMKKLALYLVVAAVAAACASKEAKQEPTGVTDRSTG
                     VANPTVGSNVTTNPTNPNMAIARNPLTDPSNALYKRSVYFDYDSNAVKDEYRGLVQAH
                     SRYLADKRDARIRIEGNCDERGSREYNLALGQRRAESVKKIMTVLGVPDARVETVSYG
                     EEKPVAPGHDEAAWAQNRRADIKYAGE"
     gene            566..1405
                     /gene="ybgF"
                     /locus_tag="VF309_09605"
     CDS             566..1405
                     /gene="ybgF"
                     /locus_tag="VF309_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX7466885.1"
                     /translation="MTRAPLAAALRWIVVAGTLAATIPARAGLFDDDEARRRIDMLRN
                     ELSQQGKDNEARIQKLEENIRNIGVIELVRQIEDLNAELARLRGQLEVIANDNQQIQK
                     RQRDFYLDLDSRLKRLEGGSGAAASSSSIDVGVASAASTIPTATAHAVTKEDQAREVK
                     AYDTASNLFRRNDFASAIDAFRAFVKDYPASPLAANAGYWIGISYANMRDYRNALAAQ
                     EELIAKYPQSAKAPDALLAIAAIHFEQGDAGSARNTLEDIIARYPLSEAAGKARTRLT
                     QRR"
     gene            1409..2080
                     /gene="queC"
                     /locus_tag="VF309_09610"
     CDS             1409..2080
                     /gene="queC"
                     /locus_tag="VF309_09610"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7466886.1"
                     /translation="MKNAVVLLSGGLDSATVLAMAVADGWRCHALSIDYGQRHRVELE
                     AAARVARAQGAIEHRIVAIDLRVFGGSALTDDSIAVPTSSTPGIPITYVPARNTIFLA
                     LALAYAEVTASDAIFTGANAVDYSGYPDCRPEFLAAFEKLANLATRRAVEGEPIEVKA
                     PIVRMTKADIVRRGHELGVDFAITVSCYNADAEGRACGQCDSCRFRREGFAGAGLPDP
                     TRYRR"
BASE COUNT          396 a          777 c          747 g          332 t
ORIGIN      
        1 ctgtgttcat cgctacaagg agacccgcat gaagaagctt gccctgtacc tcgtcgtggc
       61 tgccgtggcc gccgcttgcg ccagcaagga agccaaacag gagccgacgg gcgtgaccga
      121 tcgctccacg ggcgttgcca accccacggt gggcagcaac gtcacgacga atcccaccaa
      181 tccgaacatg gcgatcgcgc gcaacccgct caccgatccg agcaacgcgc tctacaagcg
      241 cagcgtgtac ttcgactacg attccaacgc ggtgaaggac gagtaccgcg gcctggtgca
      301 ggcgcattcg cgctatctcg ccgacaagcg cgatgcgcgc atccgcatcg agggcaactg
      361 cgacgagcgc ggcagccgcg aatacaacct cgcgctgggg cagcgccgcg ccgaatccgt
      421 gaagaagatc atgacggtgc tcggcgtgcc cgatgcgcgc gtcgagacgg tgagctacgg
      481 cgaggagaag ccggtcgcgc cgggccacga cgaggcggcg tgggcgcaga accgccgcgc
      541 ggacatcaag tacgcgggtg aataagtgac ccgggcgcct ctcgcggcgg cgctgcgctg
      601 gatcgtcgtg gccggcacgc tcgcggccac gatcccggcg cgcgccggcc tcttcgacga
      661 cgacgaggcg cgccgcagga tcgacatgct gaggaacgag ctttcgcagc agggcaagga
      721 caacgaggcg cgcatccaga agctcgaaga gaatatccgc aacatcggcg tgatcgaact
      781 cgtgcgccag atcgaggacc tcaacgcgga gctcgcgcgg cttcgcgggc agctcgaggt
      841 gatcgcgaac gacaaccagc agatccagaa gcggcagcgc gatttctatc tcgatctcga
      901 ttcgcggctg aagcgcctcg agggcggatc gggcgccgcg gcgtcgtctt cctcgatcga
      961 tgtcggggtc gcaagcgcgg cctcgacgat ccccaccgca acggcacatg cggtcacgaa
     1021 agaggaccag gcgcgcgagg tgaaggccta cgacaccgcg tcgaacctct tccggcgcaa
     1081 cgacttcgcg tcggcgatcg acgccttccg cgccttcgtg aaggattacc cggcgagccc
     1141 gctcgcggcg aacgcgggct actggatcgg catcagctac gcgaacatgc gcgactatcg
     1201 caacgcgctc gccgcgcagg aagagctcat cgcgaagtat ccgcagtcgg cgaaggcgcc
     1261 tgacgcgctg ctcgcgatcg cggcgatcca tttcgagcag ggcgatgcgg gcagcgcgcg
     1321 caatacgctc gaggacatca tcgcgcgcta tcccctgtcg gaagccgcgg gcaaggcgcg
     1381 cacccgcctc acccagcgcc gctagccgtt gaagaacgcg gtcgttctcc tctcgggagg
     1441 cctcgactcg gcaacggtgc tcgcgatggc ggtggccgat ggctggcgct gccacgccct
     1501 ttccatcgac tacgggcagc gtcaccgcgt ggagctcgaa gccgcggcgc gcgtggcgcg
     1561 cgcgcaaggc gcgatcgagc accgtatcgt tgcgatcgac ctgcgcgtct tcggcggatc
     1621 ggcgctcacc gacgattcga tcgccgtgcc cacgtcgtcc acgccgggca ttccgatcac
     1681 gtacgtgccc gcgcgcaaca ccatcttcct cgcgctcgcg ctcgcctacg cggaggtcac
     1741 cgcgtcggac gcgatcttca cgggcgcgaa cgccgtggac tattcgggat atccggattg
     1801 ccgtcccgag ttcctcgcgg cgttcgagaa gctcgccaat ctcgcgacgc gccgcgccgt
     1861 cgaaggcgag ccgatcgagg tgaaggcgcc catcgtgcgc atgacgaagg ccgacatcgt
     1921 gcgccgcggc cacgagcttg gggtcgactt cgcgatcacg gtctcgtgct acaacgccga
     1981 tgccgagggc cgcgcctgcg ggcaatgcga ttcatgccgc tttcgccgcg agggcttcgc
     2041 gggcgcgggg cttcccgacc cgacccgcta tcgaaggtaa aatagcggcc cctcgcgaaa
     2101 gattcgtcct tggccggcag cacttcccac ctcgcatccc tcgtcgccct cggcggcttc
     2161 gcgctcgcgt tcgtcttcgg cgcggtggcg aaccgcacca acttctgcac gatgggcgcg
     2221 gtctccgacg tggtgaacat gggcagctgg gg
//
LOCUS       DASVII010000737         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_214018,
            whole genome shotgun sequence.
ACCESSION   DASVII010000737 DASVII010000000
VERSION     DASVII010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214018"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VF309_09615"
     CDS             <1..521
                     /locus_tag="VF309_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7466887.1"
                     /translation="SAGQAAVFLSSGCRHVHILVRSSGLSESMSRYLIRRIEESPNIT
                     LHTRTEIASLHGAAQLERVTWRSATEPKEETRDIRDVFLMTGAVPNTRWLEGCVRLDD
                     KGFVCTGDDLGEADLQPAQWRLARRPYAYETNLPGVFAVGDVRANSVKRVASAVGEGS
                     ACVQAVHRVLSE"
     gene            complement(530..1336)
                     /gene="xth"
                     /locus_tag="VF309_09620"
     CDS             complement(530..1336)
                     /gene="xth"
                     /locus_tag="VF309_09620"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7466888.1"
                     /translation="MKNLKLATFNVNGIRTRLPHLLEWLRRESPDVACLQELKAHNET
                     LPVAEIRDAGYGAIWHGQKSWNGVAILSKGSQPIETLRGLPGDPSDEQSRYLEAAVHG
                     VIVGCLYLPNGNPQPGPKFDYKLAWFERLITHAQAHASSGHPVVLAGDFNVVPTDFDI
                     YDPKSWLKDALLQPESRERYRRLLGQGWTDAIRAKHPEERIYTFWDYFRKHWERNAGL
                     RIDHLLLNPVVAPRLVEAGVDTWVRALEKASDHAPTWITLDMSPARRSAR"
     gene            1433..1696
                     /locus_tag="VF309_09625"
     CDS             1433..1696
                     /locus_tag="VF309_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466889.1"
                     /translation="MIATEKLKKDLVNYIASFSPTRDRVFTIRDFNSQVMAMTFDAAA
                     RDGLAVSLDELVADGILERRAPTDYSLTEKGLAFVRDHRLAKG"
     gene            complement(1703..>2075)
                     /locus_tag="VF309_09630"
     CDS             complement(1703..>2075)
                     /locus_tag="VF309_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466890.1"
                     /translation="ERETQVAREFEGRLEVIDAGIARLEELEKAREIPEEVSVRHRHQ
                     LEHARRTLRRQLAGQKDPAIEKIAEREHFSNAQAVDAQRAKLLQLRSDGKIDDDIVHR
                     IERDLDEEERRLQGEEATLRG"
BASE COUNT          319 a          697 c          682 g          377 t
ORIGIN      
        1 actcggcggg gcaggccgcg gtattcctct cgagcggctg ccggcacgtg cacatcctgg
       61 ttcgttcgtc gggcttgtcg gagagcatgt cgcgctacct catccggcgc atcgaggaga
      121 gcccgaacat cacgctgcac acgcgaaccg agatcgcatc gcttcacggc gccgcacagc
      181 tcgagcgcgt cacgtggcgc agcgccacgg aaccgaagga ggaaacgcgc gacatccggg
      241 acgtcttcct catgacgggc gccgtgccca acacgcgctg gctcgaggga tgcgttcgcc
      301 tcgatgacaa gggcttcgtc tgcaccggcg acgatctcgg cgaggcggac ctgcagcccg
      361 cgcaatggcg cctcgcgcgc aggccctacg cctacgagac caacctgccc ggcgtgttcg
      421 ccgtcgggga cgtccgcgcg aacagcgtga agcgcgtcgc ctccgccgtc ggcgagggat
      481 cggcctgcgt gcaagccgtg caccgcgttc tctcggagtg acgcggcgct cagcgcgcgg
      541 agcgcctggc cggcgacatg tcgagcgtga tccacgtcgg cgcgtggtcg ctcgccttct
      601 cgagcgcgcg cacccacgtg tcaacgcccg cttcgacgag gcgcggcgcg accaccgggt
      661 tgaggagcag atggtcgatg cgaaggcccg cgttgcgctc ccaatgcttg cggaaataat
      721 cccagaacgt atagatgcgc tcttccggat gcttcgcgcg aatcgcgtcg gtccaaccct
      781 ggccgagaag gcgccggtag cgctcgcggc tctccggctg caggagcgcg tccttcagcc
      841 atgacttggg atcgtagatg tcgaaatccg tgggcacgac gttgaagtcg ccggcgagca
      901 ccaccggatg gccgctcgac gcgtgcgctt gcgcatgggt gatgagccgc tcgaaccagg
      961 cgagcttgta gtcgaacttc ggtccgggtt gcggattgcc gttcggcagg tagaggcatc
     1021 ccacgatcac gccgtgcacc gccgcctcga gatagcggct ctgctcgtcg gacggatcgc
     1081 ccggcagccc gcgcagagtc tcgatcggct gcgatccttt cgagaggatc gcgacgccat
     1141 tccaggactt ctgcccgtgc cagatcgcgc cgtatcccgc gtcgcggatc tcggccacgg
     1201 ggagcgtctc gttgtgcgcc ttcagctcct gcaggcaggc gacgtcgggc gattccctgc
     1261 gcagccactc gaggaggtgc ggcagccgcg tgcggatccc gttcacgttg aaagtggcga
     1321 gcttgaggtt cttcatgggt gtttcggggg attatcgacg aaatttccgc gattcggcgg
     1381 gcgggggcgc ggcgttcgtc ataaaatgga atcctattgg aaggaagttg ccatgatcgc
     1441 gaccgagaaa ctgaagaaag acctcgtgaa ctacatcgcg tcgttcagtc ccacgcgcga
     1501 ccgggtgttc accatccggg atttcaattc gcaggtgatg gcgatgacct tcgacgcggc
     1561 cgcgcgcgac gggttggcgg tatccctcga tgagctcgtc gccgacggca tcctcgagcg
     1621 ccgcgcgccg accgactatt ccctcacgga aaagggtctc gcgttcgtgc gcgatcatcg
     1681 cctcgcgaag ggctaggccg cctcaccctc gcagggtcgc ctcctccccc tggagccttc
     1741 gctcctcctc gtcgagatcg cgctcgatgc gatgcacgat gtcatcgtct atttttccgt
     1801 cgcttcgcaa ctgcagcagc tttgcgcgct gcgcgtcgac cgcctgggcg ttcgagaaat
     1861 gctcgcgctc ggcgatcttc tcgatcgcgg gatccttctg gccggcgagc tggcgcctca
     1921 gggtccggcg cgcatgctcg agctggtggc ggtgccgcac gctcacttcc tcgggaattt
     1981 cgcgcgcctt ctcgagctcc tcgagacgcg cgatgccggc gtcgattact tccaggcgcc
     2041 cctcgaactc gcgcgcgacc tgcgtttcgc gctcc
//
LOCUS       DASVII010000738         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_35235, whole
            genome shotgun sequence.
ACCESSION   DASVII010000738 DASVII010000000
VERSION     DASVII010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35235"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF309_09635"
     CDS             complement(<1..263)
                     /locus_tag="VF309_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7466891.1"
                     /translation="MSFLSLAPSRQADPVADAKVHLAAAHRMAVLHELEEGIDNHFTM
                     TVPGRDDRYLILPFGLHWSEARASDMIVWDESGTTLEGTGDVER"
     gene            complement(265..1233)
                     /locus_tag="VF309_09640"
     CDS             complement(265..1233)
                     /locus_tag="VF309_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7466892.1"
                     /translation="MKRLSRFAAVAAFAALCCTATASAVDYPTRTVKWVVGYPPGGTT
                     DVLARIMAQWLSEKLGQQFIIENKPGGGNNIGTEFVVNAPPDGYTMLLVNPANGINAT
                     LYKNLSFNFVRDIAPVAGIVRTPNVMEVTASFPAKTVAEFIAYCKKNPGKINMASSGS
                     GTSVHLSGELFKSMTGCDMLHVPYKGAGPALSDLIAGQVNVMFDNLPSSAPHIKGGRL
                     RALGMTSTQREPSMPDVPTVGETVPGYEATAWFGVGMPKGTPRDIIEKVNAEVNRALA
                     DPKMRERLAELGGSPIPGTPEDFGKVIRDETAKWEKVVISSGAKVE"
     gene            complement(1323..>1602)
                     /locus_tag="VF309_09645"
     CDS             complement(1323..>1602)
                     /locus_tag="VF309_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466893.1"
                     /translation="WRIDRRTSAPGRWRKVKLRQYRGFAIVETLTTPRVAALKAIALS
                     MVLLAALCLQGCGSTDPKEAAAREWQRSECDRVVDRDDREKCLKRVDR"
BASE COUNT          250 a          527 c          531 g          294 t
ORIGIN      
        1 cgctcgacat cgccggtgcc ttcgagtgtt gtgcccgatt cgtcccacac aatcatgtcg
       61 ctcgcgcgcg cttccgacca gtgcagcccg aacggcagga tcaggtagcg gtcgtcgcgc
      121 ccggggaccg tcatggtgaa gtgattgtcg atgccttcct cgagctcgtg cagcaccgcc
      181 atgcgatgcg cggcggcaag atgcaccttc gcgtcggcga cgggatcggc ctggcggctg
      241 ggggcgagcg aaaggaacga cacgctactc gaccttcgcg cccgacgaga tcacgacctt
      301 ctcccatttg gccgtctcgt cgcggatcac cttgccgaaa tcctccggag tcccggggat
      361 cggcgagccg cccagctccg cgaggcgctc gcgcatcttc ggatcggcga gcgcgcggtt
      421 cacttccgcg ttgaccttct cgatgatgtc cctcggcgtg cccttgggca tgccgacgcc
      481 gaaccacgcg gtggcctcgt agcccggcac cgtctcgccg accgtcggaa cgtcgggcat
      541 cgagggctcg cgctgcgtgg aggtcatgcc gagggcgcgc aggcgcccgc ccttgatgtg
      601 gggtgcggac gagggcaggt tgtcgaacat cacgttcacc tggcccgcga tgaggtcgga
      661 gagcgcgggg cccgcgccct tgtacggcac gtgcagcatg tcgcagccgg tcatcgactt
      721 gaagagctcg ccggagaggt gcaccgaggt gccgcttccc gacgaggcca tgttgatctt
      781 gcccgggttc ttcttgcagt acgcgatgaa ctccgcgacc gtcttcgcgg gaaatgacgc
      841 ggtcacttcc atcacgttgg gcgttcgcac gatccccgcc acgggcgcga tgtcgcggac
      901 gaagttgaac gacaggttct tgtagagggt cgcgttgatg ccgttggccg gattcaccag
      961 cagcatcgtg tagccgtcgg gcggcgcgtt gacgacgaac tccgttccga tgttgttgcc
     1021 gccgccgggc ttgttctcga tgatgaactg ctggcccagc ttctccgaga gccactgcgc
     1081 catgatccgc gcgaggacgt cggtggtgcc gccgggcgga tatcccacca cccacttgac
     1141 cgtgcgcgtg ggatagtcga cggccgatgc ggttgcggtg cagcaaagcg ccgcgaatgc
     1201 ggcaacggcg gcgaaacggg aaagacgctt cacggactac ctcctcgatc ggatggtctg
     1261 gggatgcgct ctcgaggcgc gtgcgccatg gtagcgaaac gcgggcgcgc gcgacagccc
     1321 ggtcagcgat ctacccgctt caggcacttc tcccgatcat cgcggtccac gacgcggtcg
     1381 cattcgctgc gctgccattc gcgggcggcg gcttccttcg ggtcggtgct gccgcatccc
     1441 tgcaggcaca gggcggccag caagaccatc gatagcgcaa ttgccttcaa ggcggccact
     1501 cgtggagtcg tcaaggtctc cacgatagca aaacctcggt actgtctcag cttgaccttc
     1561 cgccatcggc caggtgcgga cgttcgtcta tcgatgcgcc at
//
LOCUS       DASVII010000739         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_70776, whole
            genome shotgun sequence.
ACCESSION   DASVII010000739 DASVII010000000
VERSION     DASVII010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70776"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF309_09650"
     CDS             complement(<1..498)
                     /locus_tag="VF309_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7466894.1"
                     /translation="MDRIAGRLADGLADLERRDLKRVRRVIDSPQGPRVRVAGRALVN
                     FSSNDYLGLANHPRLRDAAHRAIDEHGVGSGASPLVSGNLRAHHEAERRFARFAGLPQ
                     ALLFGSGYAANLGILAALADRDAEIFSDELNHACLIDGARLSKAKVTRFPHRDVDALE
                     ARLAAS"
     gene            complement(504..1484)
                     /gene="bioB"
                     /locus_tag="VF309_09655"
     CDS             complement(504..1484)
                     /gene="bioB"
                     /locus_tag="VF309_09655"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX7466895.1"
                     /translation="MDTPVRFHPAAEPATAERTSARARALELYAQPLNDLLFEAHTVH
                     REHFDPNRVQRSTLLSIKTGGCPEDCGYCPQAARYHTGVESQDLLPLSKVIDAARAAK
                     EHGATRFCMGAAWRGPKARDLAPVLDMVREVKALGLETCCTLGMLKDGQAEALRDAGL
                     DYYNHNLDTAPEHYGAIVTTREYRDRLDTLARVRFAGVKVCCGGIVGLGESREARAGL
                     LAQLASLDPQPESVPINDLVQVEGTPLDGVEKLPWVEFVRTIAIARILMPRSMVRLSA
                     GRAEMSEAVQAMCFFAGANSIFYGEKLLTTGNPDVARDDALFCALGIESA"
BASE COUNT          260 a          527 c          569 g          267 t
ORIGIN      
        1 cgacgccgcg agtcgagcct cgagggcgtc gacgtcgcga tggggaaagc gcgtgacttt
       61 cgctttcgac aggcgcgcgc cgtcgatgag gcacgcgtgg ttgagctcgt cggaaaagat
      121 ctccgcatcg cgatccgcga gagccgccag gattccgagg ttcgccgcat agccgctgcc
      181 gaagaggagc gcttgcggga gaccggcgaa acgcgcgaag cgccgctcgg cctcgtgatg
      241 cgcgcgcagg ttgcccgaga ccaggggcga agcgcccgag cccacgccgt gctcgtcgat
      301 cgctcgatgg gccgcatcgc gaaggcgcgg atggttcgcg aggccgaggt agtcgttgct
      361 cgagaagttg acgagggcgc ggccggcaac gcgaacgcgc ggaccctgcg gggaatcgat
      421 gacgcggcgc acgcgcttca ggtcgcggcg ctcgagatcg gcgaggccgt cggccagccg
      481 gcccgcgatg cggtccaccg tcgtcaggcc gactcgatgc cgagcgcgca gaagagcgcg
      541 tcgtcgcgcg cgacgtccgg attgcccgtg gtgaggagct tctcgccgta gaagatcgaa
      601 ttggcgccgg cgaagaagca catcgcctgc accgcttcgc tcatttccgc gcggccggcc
      661 gacaggcgca ccatgctgcg cggcatcagg atgcgcgcga tcgcgatggt gcgcacgaac
      721 tcgacccaag gaagcttctc gacgccgtcg agcggcgtgc cctcgacctg cacgaggtcg
      781 ttgatcggta cggactcggg ctgcggatcg aggctcgcga gctgcgcgag cagccccgcg
      841 cgcgcttcgc gcgattcgcc gaggcccacg atgccgccgc agcagacctt aacgcccgca
      901 aagcgaacgc gggccagcgt atcgaggcga tcgcggtatt cgcgcgtcgt gacgatcgcc
      961 ccatagtgct cgggcgccgt gtcgaggttg tggttgtagt agtccagccc ggcatcccgc
     1021 agcgcctcgg cctggccgtc cttcagcatc ccgagcgtgc agcaggtctc gagcccgagt
     1081 gccttcactt cgcgcaccat gtcgagcacc ggggcgagat cgcgcgcctt gggcccgcgc
     1141 cacgccgcgc ccatgcagaa gcgggtggcg ccatgctcct tcgcggcccg tgccgcgtcg
     1201 atcactttcg agagcggaag caggtcctgg ctctcgacac ccgtgtgata gcgtgcggcc
     1261 tgcgggcagt agccgcagtc ttcgggacag ccgccggtct ttatcgagag cagcgtcgat
     1321 cgctgcacgc ggttcggatc gaaatgctcg cgatgcacgg tatgcgcttc gaacagcaga
     1381 tcgttgagag gctgcgcgta caactcgagc gcccgtgcgc gagcgctcgt ccgctcggcg
     1441 gtcgcgggtt cggcagcggg gtggaacctg acgggcgtgt ccataggtag ggattcaata
     1501 acacgctgat atttattgtt tttcgcctca atgtcaaatt tctcagcggt tcaaacctta
     1561 cgggtgctca aagtttcggc agcgcgctgc tggggcgagg attgcctgct ttgcgcagct
     1621 ccc
//
LOCUS       DASVII010000740         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_70810, whole
            genome shotgun sequence.
ACCESSION   DASVII010000740 DASVII010000000
VERSION     DASVII010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70810"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VF309_09660"
     CDS             complement(<1..1043)
                     /locus_tag="VF309_09660"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX7466896.1"
                     /translation="MSDLDPRSVGAAVAQRAGFLEPLILKAGKTLASYELVYETYGKL
                     NADRSNAILVCHALSGSHHVAGFYADDPKKVGWWDNLIGPGKAIDTDRFFVIGVNNIG
                     GCHGSTGPASVDRATGKPYGAAFPVVTVEDWVVSQARLMDRLGIAKLAGAIGGSLGGM
                     QALQWTLTFPDRIGHAFVIAAAPRLSTQNIAFNDVARQAIVTDPDFHGGNFYAHQAVP
                     ARGLKLARMLGHITYLSEDMLMEKFGRVLKRSELGFNYDVEFEIESYLRYQGDKFAQV
                     FDANTYLLMTKALDYFDPAKESGGNLARALSAARAGFFVASFKSDWRFPPQRSRELVK
                     ALVASKKAVTYAE"
     gene            1289..>1861
                     /locus_tag="VF309_09665"
     CDS             1289..>1861
                     /locus_tag="VF309_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466897.1"
                     /translation="MRFILAALLVAAIGSASAADEDRVYAIASLLGDGLSVVVRENTT
                     GSNINRNARTFLRLETPALDRAALFAADDAIKGASPGAKRVMLEVHDPEVYAAQSRAL
                     DEGGDIRPVLLTLGATLAQSKATHLVLFTKQRHEAMLRLRDGNVGNGRLEGLGFYVDR
                     AMRTQNPDTGEYYVGFLSPFAYFRVSLIEAS"
BASE COUNT          316 a          611 c          613 g          321 t
ORIGIN      
        1 atctccgcgt aggtgaccgc cttcttcgag gcgacgaggg ccttcaccag ctcgcgcgag
       61 cgctgcggcg gaaagcgcca gtcgctcttg aaggacgcga cgaagaatcc cgcccgcgcc
      121 gccgatagcg cccgcgccag gtttccgccg gactccttcg cgggatcgaa atagtcgagc
      181 gccttcgtca tcagcaggta cgtgttcgcg tcgaacacct gcgcgaactt gtcgccctgg
      241 tagcgaaggt acgactcgat ctcgaactcc acgtcgtagt tgaagccgag ctcgcttcgc
      301 ttgaggacgc gcccgaactt ctccatcagc atgtcctcgg acaggtaggt gatgtggccg
      361 agcattcgcg cgagcttgag gccccgcgcc ggcaccgcct ggtgcgcgta gaaattgccg
      421 ccgtggaaat cgggatcggt gacgatcgcc tggcgcgcga cgtcgttgaa cgcgatgttc
      481 tgcgtggaca gccgcggcgc ggccgcgatg acgaacgcgt gcccgatgcg atcgggaaag
      541 gtgagcgtcc actgcagcgc ctgcatgccg cccagcgatc cgccgatcgc gccggcgagc
      601 ttcgcgatgc cgaggcgatc catgaggcgc gcctgcgaca cgacccagtc ctccacggtc
      661 accacgggga acgccgcgcc gtagggcttg ccggtcgcgc ggtcgaccga ggcaggcccc
      721 gtggagccgt ggcatccgcc gatgttgttc acgccgatca cgaagaagcg atcggtgtcg
      781 atcgccttgc cggggccgat gaggttgtcc caccagccga ccttcttcgg atcgtcggcg
      841 tagaagcccg cgacatggtg gcttcccgac agcgcatggc acacgaggat cgcgttggac
      901 ctgtcggcgt tgagcttgcc gtacgtttcg tagacgagct cgtacgaggc gagggttttc
      961 cccgccttga ggatgagggg ctcgaggaag ccggcgcgct gcgccaccgc ggctcccacc
     1021 gagcgaggat cgagatcgct catgtggaaa taagaaaccc gacggacttg aaggtcgtgt
     1081 cgggtttttg gcgccgccac tcgctttagc cgtatttgtt aagcgcccgc aagctgaggt
     1141 caaattggcg ctgaatgtcc attctatgcc gatgctggac cggcttcaag gcgccacggg
     1201 cccagcacgc gttcccaccc gcgcgggtag gcgatcgcgg ggtccaggcg gatatcatct
     1261 cgcatcgtcg cagatcgaaa ggactcacgt gcgcttcatc ctcgcagccc tccttgttgc
     1321 cgccatcggc tccgccagcg ccgccgacga agatcgcgtc tacgcgatcg cctcgcttct
     1381 cggcgacggc ctgtcggtcg tggtgcgcga gaacaccacc ggcagcaaca tcaacaggaa
     1441 tgcgcgcacg ttcctcaggc tcgagacgcc ggcgctcgac cgcgctgcgc tgttcgcggc
     1501 cgatgacgcg atcaagggtg cctcgccggg cgcgaagagg gtgatgctcg aggtgcatga
     1561 tccggaggtc tacgccgcgc agtcccgagc gctcgacgaa ggaggagaca tccgccccgt
     1621 gctgctcacg ctcggggcga ccttggccca gtcgaaggcg acccatctcg tgctcttcac
     1681 caagcagcgc cacgaagcga tgctgcgtct gcgggacggg aacgtcggca acggccgcct
     1741 cgaggggctc ggattctacg tggaccgcgc gatgaggacg cagaacccgg ataccgggga
     1801 atactacgtg gggttcctgt cgccgttcgc gtatttccgc gtatcgctca tcgaagcctc
     1861 c
//
LOCUS       DASVII010000741         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_178080,
            whole genome shotgun sequence.
ACCESSION   DASVII010000741 DASVII010000000
VERSION     DASVII010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178080"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1053
                     /locus_tag="VF309_09670"
     CDS             73..1053
                     /locus_tag="VF309_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7466898.1"
                     /translation="MKGITMKWRFARTLGLSLALACGGALADSFPTRPVRIIVPFAPG
                     GNLDVTARIVAESMAKELGQPFIVENRPGAGGTIGSDAVAKSPPDGYTLVAGTTATSI
                     VSPLLVPNPPYELQSFAPIGMMAVTPLVLEVPAASPHKDFRSYVAYVRANPGKVTIGH
                     SGNGTTNHVAILLMQDALKVSWNVIPYKGSGPALVDLIGGQIDSMMDQTSSSLPQIQG
                     GKLRALAVGTRTRVADLPNVPTLAEEGMKDFEAVTPSGLFAPARTPADVVGILNAALN
                     KALADPVIRKRLADLGSEPRPMSAEQFDQILKEEERKFKALARSGVLKGE"
     gene            complement(1082..1381)
                     /locus_tag="VF309_09675"
     CDS             complement(1082..1381)
                     /locus_tag="VF309_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX7466899.1"
                     /translation="MNDPAAINDDVTLRLSIRGRVQGVWFREAMRREAERLAVRGWVC
                     NRHDGSVEAVVQGSRAAVDKIVRWAHRGPEDARVSGVEVTEAEGEFAGFEKRPGH"
     gene            complement(1392..1763)
                     /locus_tag="VF309_09680"
     CDS             complement(1392..1763)
                     /locus_tag="VF309_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7466900.1"
                     /translation="MPSREPQRPPPYATDREVLEREVDIEVFRSSGPGGQHVNKTESA
                     LRIIHRPSGVVVISRDSPSQHRNRETAFERLVERLVRLNHVPKKRVATKPSRAAKQRR
                     IEAKKIRTVTKRTRSRVRDDE"
     gene            1940..2131
                     /locus_tag="VF309_09685"
     CDS             1940..2131
                     /locus_tag="VF309_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466901.1"
                     /translation="MNTKQQLATGFLALVIVGSVTFMPVSAGQKDSTAAAPRAQATTI
                     ATLNTTELPQEQVKDLTYN"
     gene            complement(2194..>3629)
                     /gene="metG"
                     /locus_tag="VF309_09690"
     CDS             complement(2194..>3629)
                     /gene="metG"
                     /locus_tag="VF309_09690"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX7466902.1"
                     /translation="FGIEIPNAPGKYFYVWLDAPIGYLASLRNYLEKKGQDFAKYLAD
                     PKVEQIHFIGKDIIYFHTLFWPAMLKYSERKVPDNVYVHGFLQLSGEKMSKSRGVSVS
                     PQRYIDIGLNPEWIRYYFAAKLNAHVEDMDFNPDDFLARINSDLVGKYVNIASRAAGF
                     IGKRFGGVLSEEPLSAGRELKHKMRGNLAAPVPDDEQAPAILFEVRSFADAIAADYEA
                     REFSKAIRQIMFLADRVNQYVDEQKPWEIAKEAGQDAALQWFCTLYLELFRILTIYLK
                     PVLPKVAEEVEEFLALKPLAWEDVGTPLKTGHKVQPYRHLVSRIDSKQIDELLEPPAE
                     AKPEPPAKASVVAEAPEFKLEPIEPPRPPVILPVGDTISIDDFSKIDLRIARIVDAEL
                     VPDADKLLKLTLDIGIESNRTVFAGIRTVYTPKDLIGRLTVMVANLAPRKMRFGESQG
                     MVLAAGDGNSIYLLAPDSGAMPGMRIK"
BASE COUNT          605 a         1180 c         1223 g          621 t
ORIGIN      
        1 ctggctcggt ccccaggacc tggagtggat catgggccgc ggtgtctgcg aatggctcgg
       61 ctggaaaacc tgatgaaagg aatcacgatg aagtggcgat tcgcacgaac cctgggactg
      121 tcgctcgcgc tcgcgtgcgg cggcgcgctc gcggactcgt ttcccacccg gccggtgagg
      181 atcatcgtgc cgttcgcgcc gggcggaaac ctcgacgtca cggcgcgcat cgtcgccgag
      241 tcgatggcga aggagctggg ccagccattc atcgtggaaa atcgtcccgg cgccggcggc
      301 acgatcggaa gcgacgcggt ggcgaagtcg ccgcccgacg gctatacgct ggtcgcggga
      361 acgacggcga cctcgatcgt gagcccgctt ctcgttccga acccgcccta cgagctgcag
      421 agtttcgcgc ccatcgggat gatggcggtc acgccgctcg tgctcgaggt gcccgcggcg
      481 agcccgcaca aggacttcag gtcgtacgtc gcctacgtga gggccaaccc gggcaaggtc
      541 accatcggcc actccggaaa cggcacgacc aaccacgtgg cgatcctgct catgcaggac
      601 gcgctcaagg tgagctggaa cgtgattccg tacaagggat ccggtcccgc gctggtcgac
      661 ctcatcggag ggcagatcga ttcgatgatg gaccagacct cgagctcgct gccgcagatc
      721 cagggcggaa agctgagggc gctggcggtc ggcacgagaa cgcgcgtcgc ggatctcccc
      781 aacgtgccga ccctcgccga ggagggcatg aaggacttcg aggcggtgac gccgagcggg
      841 ttgttcgcgc ccgcgagaac gcccgccgat gtcgtgggga tcctcaacgc cgcgctcaac
      901 aaggccctcg ccgatcccgt catccgcaag cgccttgccg atctcgggtc ggagccccgg
      961 cccatgtccg ccgagcagtt cgaccagatc ctcaaggagg aggaaaggaa gttcaaggcg
     1021 ctggccaggt ccggcgtctt gaagggcgaa tgatcgtcgc gccgggagtg gcgaggccgg
     1081 cctagtggcc cggcctcttc tcgaagcccg cgaactcgcc ttccgcctcc gtgacttcga
     1141 cgccggacac ccgcgcgtcc tccggcccgc gatgggccca gcggacgatc ttgtcgacgg
     1201 ccgcgcggga accctgaacc accgcctcga cgctgccgtc atggcgattg cacacccagc
     1261 ccctcaccgc gaggcgctcg gcctcgcggc gcatcgcctc gcgaaaccag acgccctgca
     1321 cgcgcccgcg aatcgacaag cgtagcgtca cgtcgtcatt gatggccgca gggtcattca
     1381 tccccgcggg ctcattcatc gtcgcgtacg cgcgagcgcg tgcgtttggt cacggtgcgg
     1441 atcttcttcg cctcgatgcg ccgctgcttc gcggcgcggg agggcttagt cgccacgcgc
     1501 ttcttcggca cgtggttcag ccgcaccagg cgctccacca ggcgctcgaa ggcggtctcg
     1561 cgattgcggt gctgcgaagg cgagtcgcgg gagatcacca ccacgcccga cggccgatgg
     1621 atgatccgca gcgccgattc ggtcttgttc acgtgctggc cgcccggccc gctggaacgg
     1681 aagacctcga tgtcgacctc ccgctcgagg acctcgcgat cggtcgcgta gggcggagga
     1741 cgctggggct cacgggaggg catggcgccg attctcgggc attttcgggc gcagcggcgc
     1801 ggcgccgcca ttcttacaac tgtatttccg cgccgtgggc ttttgtgact gcgttaacag
     1861 gcacggcggc gcgatcggag aaagatgggt cccgtggtcg agcggaaacg cagccgtcaa
     1921 acagccaagg gagaacaaat tgaatacgaa gcagcagcta gctacaggat tcctcgcact
     1981 tgtgatcgtg ggcagcgtca ctttcatgcc ggtgagcgcc gggcaaaagg actcgaccgc
     2041 cgccgcgccc cgcgcgcagg ccacgacgat cgccacgctg aacaccaccg agctgccgca
     2101 ggagcaggtc aaggacctca cgtacaactg atcgattcac ccgcaaggcg cacttgcgaa
     2161 tggccgccgg ctccgccggc ggcttttttt cgcctatttg atgcgcatcc ccggcatggc
     2221 gccggaatcg ggggcgagga gatagatcga gttgccgtcg cccgcggcga gcaccatgcc
     2281 ctgggattcg ccgaagcgca tcttgcgcgg ggcgagattc gcgaccatca ccgtgagcct
     2341 gccgatgagg tccttgggcg tgtagaccgt gcggattccc gcgaacaccg tgcgattcga
     2401 ctcgatgccg atgtcgagcg tgagcttgag gagcttgtcc gcgtcgggca cgagctcggc
     2461 atcgacgatg cgcgcgatgc gcaggtcgat cttggagaag tcgtcgatcg agatcgtgtc
     2521 gcccacgggc aggatcaccg gaggccgcgg cggctcgatg ggctcgagct tgaactccgg
     2581 agcctccgcg acgacgctcg ccttggcggg cggctcgggc ttcgcctcgg cgggcggctc
     2641 gaggagctcg tcgatctgct tcgagtcgat gcgcgagacg agatggcggt acggctgcac
     2701 cttgtggccg gtcttgagcg gcgtgccgac gtcttcccac gccaggggct tcagcgcgag
     2761 aaactcctcg acctcctcgg cgaccttcgg cagcacgggc ttcaggtaga tcgtgaggat
     2821 gcggaagagc tcgagataga gcgtgcagaa ccactgcagc gccgcgtcct ggcccgcttc
     2881 cttggcgatc tcccacggct tctgctcgtc gacgtactgg ttcacgcggt cggcgaggaa
     2941 catgatctgg cggatcgcct tgctgaactc gcgcgcctcg tagtccgcgg cgatcgcgtc
     3001 ggcgaagctg cgcacctcga acaggatcgc cggcgcctgc tcgtcgtcgg ggaccggggc
     3061 ggcgaggttg ccgcgcatct tgtgcttgag ctcgcgcccg gccgacaggg gctcttccga
     3121 gagcaccccg ccgaagcgct tgccgatgaa gcccgcggcg cggctcgcga tgttcacgta
     3181 cttgccgacg aggtcggaat tgatgcgcgc caggaaatcg tcgggattga agtccatgtc
     3241 ctcgacgtgg gcgttcagct tcgccgcgaa gtagtagcgg atccattccg ggttgaggcc
     3301 gatgtcgatg tatctctgcg ggctcacgga cacgcctcgc gacttcgaca tcttctcccc
     3361 cgaaagctgc aggaagccgt gcacgtagac gttgtcgggc accttgcgct ccgagtactt
     3421 gagcatcgcg ggccagaaca gcgtgtggaa atagatgatg tccttgccga tgaagtggat
     3481 ctgctccacc ttcgggtcgg cgaggtactt cgcgaaatcc tggcccttct tctcgaggta
     3541 gttcctgagc gaggcgaggt agccgatcgg cgcgtcgagc cacacgtaga agtattttcc
     3601 cggcgcgttg gggatctcga tcccgaagt
//
LOCUS       DASVII010000742         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_322063,
            whole genome shotgun sequence.
ACCESSION   DASVII010000742 DASVII010000000
VERSION     DASVII010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322063"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..348)
                     /locus_tag="VF309_09695"
     CDS             complement(79..348)
                     /locus_tag="VF309_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466903.1"
                     /translation="MSETLKKAGVCPHCGAPLKTLAPDIPGKAPDPAVALDPSKAFFA
                     DFVRYDLETLAAEAQQEKAFDESARVRVTQQDIRQLLEKRRKGRK"
     gene            complement(345..803)
                     /locus_tag="VF309_09700"
     CDS             complement(345..803)
                     /locus_tag="VF309_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7466904.1"
                     /translation="MDIKATLLSPAGKAPVSPPGRKIRVLIADDEFHVRQIVAGIVRA
                     LGAVVVAEAGDGDEAVEAFERLRPDLVILDINMPRMRGDEALTRILAIDPQVVAIMMT
                     AQDTIDSVQECLDRGARHYILKSNRAEEIMRLMTEVWPDCIAHVRSTESK"
     gene            complement(904..1413)
                     /locus_tag="VF309_09705"
     CDS             complement(904..1413)
                     /locus_tag="VF309_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7466905.1"
                     /translation="MKILIAVDGSPYTKAAARYVVRHRESFANPLEIHLLHVQSPIPY
                     PGAAAVAGKSAVESYQRDESLAALAPAEKELAKAGVAHTSAWRVGDVAQTIEKYAREQ
                     NIDLVISGSHGHGALASLAMGSVSTKLVATLSVPLLIITRGAAARADRKPGEGLGDAA
                     GQREAVATP"
BASE COUNT          238 a          514 c          566 g          289 t
ORIGIN      
        1 tcgcgagacc ctcgagctgc gcggcgaacg tcgggcagag gtcggacgcc ttcttgcgga
       61 tctcggggct gatggcgatc atttgcggcc cttcctgcgt ttctcgagca gctggcggat
      121 gtcctgctgg gtcacccgca cgcgcgcgga ttcgtcgaag gccttttcct gctgggcttc
      181 ggcggccagg gtctccaggt cgtagcgaac gaaatccgcg aagaaggcct tcgagggatc
      241 gagagcgacc gcgggatcgg gcgccttgcc gggaatgtcc ggcgcaaggg tcttcagcgg
      301 cgcgccgcag tgcgggcata cgcccgcctt cttcagggtc tcgctcattt gctctccgtg
      361 cttctcacgt gcgcgatgca atcgggccag acctcggtca tgagccgcat gatctcctcg
      421 gcgcgattgc tcttgaggat gtagtggcga gccccgcgat cgaggcattc ctgcaccgag
      481 tcgatggtgt cctgggcggt catcatgatg gcgaccacct ggggatcgat ggcgaggatg
      541 cgcgtgagcg cctcgtcgcc gcgcatcctc ggcatgttga tgtcgaggat gaccaggtcg
      601 gggcgcaggc gttcgaaggc ctccacggcc tcgtcgccgt cgcccgcttc ggccacgacc
      661 acggcgccga gcgcgcgcac gatcccggcg acgatctggc gcacgtggaa ctcgtcgtcg
      721 gcaatgagaa cgcggatctt gcggcccggg ggactcacgg gcgcctttcc ggctggggac
      781 agaagcgtcg ctttgatgtc catgttgcag tatcccgctg cctggattgc cccctttgtc
      841 cccgatcaat tgctagccga tacgtggagg tggcgtcccg cgcgaagtcg gggatgagtc
      901 gctctacggg gtggctacgg cctcgcgctg cccggctgca tccccgagac cctcgcccgg
      961 cttgcggtcg gcccttgccg cggccccgcg cgtgatgatc aacaggggca cgctgagcgt
     1021 tgcgacgagc ttggtggaca cggatcccat cgcgaggctg gccagcgcgc cgtggccgtg
     1081 ggagcccgag atcaccagat cgatgttctg ttcccgcgcg tacttctcga tcgtttgcgc
     1141 cacgtcaccg acgcgccagg cggaggtgtg cgcgacgccc gccttggcga gctccttctc
     1201 cgcgggcgcg agcgccgcga ggctttcgtc gcgttggtag ctctccaccg cggacttgcc
     1261 ggcaacggcc gcggcccccg ggtaggggat cggcgattgc acatggagca ggtggatttc
     1321 cagcgggttc gcgaacgatt cccgatgccg cacgacatag cgggcagcgg ccttcgtgta
     1381 gggggaaccg tcgactgcga tgaggatctt catggaagcc ctttcttcgg aaacgcctgc
     1441 gtacgatcag tgtggcaaga agcccgctga gggagttgat gccaatcaat ggcgcgccgg
     1501 gcggcggcgc gcgaccttgt agaggtcgta ccagaggacc gagacgaggc cgagcgccgc
     1561 ggcgcccgcg agatcggccg ccgggagcgg cacgaacctg aaaagcg
//
LOCUS       DASVII010000743         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_178320,
            whole genome shotgun sequence.
ACCESSION   DASVII010000743 DASVII010000000
VERSION     DASVII010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178320"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VF309_09710"
     CDS             complement(<1..355)
                     /locus_tag="VF309_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7466906.1"
                     /translation="MSSQKILTGFHAVTSRLRQRPETVTEIYLDAGRSDARAKDLREL
                     AKRLNVRLISADMKRLDGMANGVRHQGVIAHAEPLDTPKFIEDVLEAIDEPPLLLILD
                     GVQDPHNLGACLRVAD"
     gene            complement(352..>2373)
                     /gene="rnr"
                     /locus_tag="VF309_09715"
     CDS             complement(352..>2373)
                     /gene="rnr"
                     /locus_tag="VF309_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX7466907.1"
                     /translation="RRLRAMEREGQVVRNRRGAVLVADKAGLIKGKVIGHPDGFGFLR
                     PETGKEDDLFIGPREMHKVLHGDVVLGRISGVDRRGRSEVNIVEVLERANQKVVGRLF
                     IEHDVAFVIAENKRISQDILLMPDSLKGAKPGQVVVAEIVEQPSKNAEPIGRVVEILG
                     NYADPGMEIEIALRKHDLPHEWPADVEAAARRIGSKVEPKDLKGRKDLRKLAFVTIDG
                     ETAKDFDDAVYAERVGKNFKLWVAIADVSHYVHPGDALDQEAYERGNSVYFPRRVIPM
                     LPEELSNELCSLKPDVDRLVMVCEMEVTAGGVVKAYEFHPGVIHSHARLTYTRVAAAL
                     AGQPADPPVARELVPQLEILYGVFKALFSQRQKRGAIDFESVETQMIFDDHGKIERIV
                     RVERNDAHRLIEECMLAANVSASDYLEKSDQPTLYRVHEGPTPEKLESLRTMLKDFAL
                     TLGGGDKPHAKDYAELLKRIKDRPYAGMLQTVMLRSLHQAVYTPENVGHFGLAYDAYA
                     HFTSPIRRYPDLLVHRGIKAVVKGQRYQAGDLNAIGNHCSETERRADDATRDVEAWLK
                     CYYMQDHVGEEFEGTVSGVTNFGLFVTLDELYVDGLVHISDLGQDYFTFDAAKHTLRG
                     ERSGVKYQLAARVKVKVVRVNLEQAKIDFTLVEEKKQVSDTKVKGKKKR"
BASE COUNT          333 a          810 c          784 g          446 t
ORIGIN      
        1 cgtcggccac gcgcaggcac gcgccgaggt tgtgcggatc ctgcacgccg tcgaggatga
       61 gcaagagcgg cggctcgtcg atcgcctcca gcacgtcctc gatgaatttc ggcgtgtcga
      121 ggggctccgc gtgcgcgatg acgccctggt ggcgcacccc gttcgccatg ccgtcgaggc
      181 gcttcatgtc cgcggagatg agccgcacgt tgaggcgctt ggcgagctcg cgcaggtcct
      241 tcgcgcgcgc gtcgctcctc cccgcgtcga ggtagatctc cgtcaccgtc tccggccgtt
      301 gcctcagccg gctcgtcacc gcgtggaatc ccgtgaggat cttctggctg ctcatcgctt
      361 cttcttcccc ttgaccttgg tgtcggagac ctgtttcttc tcctccacga gcgtgaaatc
      421 gatcttcgcc tgctccaggt tcacccgcac caccttcacc ttcacgcgcg cggcgagctg
      481 gtacttcacc ccgctcctct cgccgcgaag ggtgtgcttg gcggcgtcga acgtgaagta
      541 atcctggccg agatcggaga tgtgcacgag gccgtcgacg tacagctcgt ccagcgtgac
      601 gaagaggccg aagttggtga ccccgctcac ggtgccctcg aactcctcgc cgacgtggtc
      661 ctgcatgtag tagcacttga gccacgcctc gacgtcgcgc gtcgcgtcgt cggcgcgccg
      721 ctcggtctcg gagcaatggt tgccgatcgc gttcaggtcg cccgcctggt agcgctggcc
      781 cttcaccacc gccttgatgc cgcgatgcac gaggagatcc ggatagcggc gaatgggcga
      841 agtgaagtgc gcgtaggcgt cgtaggcgag gccgaagtgg ccgacgttct cgggcgtgta
      901 caccgcctgg tgcagcgagc gcagcatcac cgtctgcagc atgcccgcgt agggccggtc
      961 cttgatgcgc ttcaggagct cggcgtagtc cttcgcgtgg ggcttgtcgc cgccgccgag
     1021 ggtgagcgcg aaatccttga gcatggtgcg caacgactcg agcttctcgg gcgtgggccc
     1081 ttcgtgcacg cgatagagcg tcggctggtc gctcttctcg agatagtccg acgcggagac
     1141 gttcgccgcg agcatgcact cctcgatcag gcggtgggcg tcgttcctct cgacgcgcac
     1201 gatgcgctcg atctttccat ggtcgtcgaa gatcatctgc gtctcgacgc tctcgaagtc
     1261 gatggcgccg cgcttctggc gctggctgaa gagcgccttg aagacgccgt agaggatctc
     1321 gagctgcggc acgagctccc tcgccacggg cgggtcggcg ggttgccccg cgagcgccgc
     1381 cgcgacgcgc gtgtaggtga ggcgcgcgtg cgagtggatc acgccgggat ggaactcgta
     1441 ggccttcacc acgccgcccg cggtgacttc catctcgcac accatcacca ggcgatcgac
     1501 gtcgggcttg agcgagcaca gctcgttcga cagctcctcg ggcagcatcg ggatcacgcg
     1561 gcgcggaaag tacaccgagt tgccgcgctc ataggcctcc tggtcgagcg cgtcgccggg
     1621 gtgcacgtaa tggctcacgt ccgcgatcgc gacccacagc ttgaagttct tgccgacgcg
     1681 ctccgcgtac accgcgtcgt cgaagtcctt cgcggtctcg ccgtcgatcg tcacgaacgc
     1741 gagcttgcga aggtccttgc ggcccttcag gtccttcggc tcgactttgg agccgatcct
     1801 gcgcgcggcc gcctcgacat cggcgggcca ctcgtgcggg aggtcgtgct tgcgcagcgc
     1861 gatctcgatc tccatcccgg gatcggcgta gttgccgagg atctcgacga cgcgcccgat
     1921 gggctcggcg ttcttcgaag gctgctcgac gatctcggcg accacgacct gtccgggctt
     1981 cgcgcccttg aggctgtcgg gcatgaggag gatgtcctgg ctgatgcgct tgttctccgc
     2041 gatcacgaag gcgacgtcgt gctcgatgaa caggcgcccg accaccttct ggttggcgcg
     2101 ctccagcacc tcgacgatgt tcacctcgct gcggccgcgg cgatccacgc ccgagatgcg
     2161 cccgagcacc acgtcgccgt gcagcacctt gtgcatctcg cgcgggccga tgaagaggtc
     2221 gtcctccttg ccggtctcgg gacgcaggaa gccgaagccg tcggggtgcc cgatcacctt
     2281 gcccttgatg aggccggcct tgtcggcgac gagcaccgcg ccgcggcggt tgcgcaccac
     2341 ctgcccctcg cgctccatgg cgcgcaggcg ccg
//
LOCUS       DASVII010000744         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_214477,
            whole genome shotgun sequence.
ACCESSION   DASVII010000744 DASVII010000000
VERSION     DASVII010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214477"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VF309_09720"
     CDS             <1..831
                     /locus_tag="VF309_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7466908.1"
                     /translation="WQSPETLNSIFVTNASGAQIPLSAFATFGPTNTPLQVSHQGQFA
                     ASTISFNLPEGVAMSQASDVIRDTMARIGVPTTIYASFEGTAKLFQQGLDNQPMLILA
                     ALLTVYIVLGILYESLVHPITILSTLPSAGVGALLALKLFNTEFSLIALIGVILLIGI
                     VKKNAILMIDFALETERKGNKDPKDAIYEAALLRFRPIMMTTMAAMLGAIPLALGSGD
                     GAELRRPLGISIVGGLLVSQLLTLYTTPVIYLYLDRLRLWARGKRRPAPASLSPQAGD
                     "
     gene            964..>1646
                     /locus_tag="VF309_09725"
     CDS             964..>1646
                     /locus_tag="VF309_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7466909.1"
                     /translation="MLLLVSGCAIGPNYSKPPVEVPSQWKEAGDWVVAQPKDASPKGK
                     WWEAFNDPVLNGLEEQVEVSNQTLAAAEARYRQARASVQSARAGFFPTIGGSAGATRS
                     RAGSTQVDANGNVSIGGSSRTSYTAGLDARWEIDLWGRIRRLVEAARAGEQASAADLE
                     AAKLSIRAELATNYFQLRVTDVTRDLLDDTVKAFQSSYDVTQNRYRAGVAGKVDVVQA
                     EAQLLSTQA"
BASE COUNT          298 a          560 c          525 g          263 t
ORIGIN      
        1 tggcagagtc ccgagaccct caacagcatt tttgtcacca acgcgagcgg tgcgcagatt
       61 cccctgtcgg cattcgcgac attcggcccg accaacacgc cgttgcaggt gagccaccag
      121 gggcagttcg cggcgtcgac catctcgttc aacctgcccg aaggcgtggc gatgtcgcag
      181 gcctccgacg tcattcgcga caccatggcg aggatcggcg tgccgaccac catctacgct
      241 tcgttcgagg gcacggcgaa gctcttccag caggggctgg acaaccagcc catgctcatc
      301 ctggccgcac tcctcaccgt ctacatcgtg ctcggcatcc tctacgagag cctggtgcac
      361 ccgatcacca tcctgtccac gctgccgtcg gccggcgtgg gcgcgctcct cgccctcaag
      421 ctcttcaaca ccgagttctc gctgatcgcc ctcatcggcg tgatcctgct gatcggcatc
      481 gtgaagaaga acgcgatcct gatgatcgac ttcgccctcg agacggaacg caagggcaac
      541 aaggatccga aggacgcgat ctacgaggcg gcgctgctgc gcttccgccc gatcatgatg
      601 acgaccatgg cggcgatgct gggcgccatc ccgctcgcgc tgggctcggg tgacggcgcc
      661 gagctgcgca ggcccctggg catctcgatc gtcggggggc tcctcgtgag ccagctcctg
      721 accctctaca ccacgcccgt gatctatctc tacctcgacc gcctgcgcct ctgggcgcgc
      781 ggcaagcgtc gtcccgcacc tgcgtctctt tcgccgcaag cgggcgacta gggcgggaac
      841 gcagatgaac gcagataaaa gcgaacgcag atgaacgtac accaggattg catggatctc
      901 gccgccatat tccagaaagg ggtacgttca tctgcgcgac atctgcgttc atctgcgttt
      961 ccaatgctct tgcttgtgag cggatgcgcg atcgggccga actactcgaa gccgccggtg
     1021 gaagtgccgt cgcaatggaa ggaagccggc gattgggtgg tcgcgcagcc aaaggacgcg
     1081 tcgcccaagg gcaagtggtg ggaagcattc aacgatcccg tgctgaacgg cctcgaggag
     1141 caagtggagg tctcgaacca gacgctcgcc gcggcggagg cgcgctaccg ccaggcacgt
     1201 gcctcggtgc agtccgcgcg cgccggcttc tttccgacga tcggcggcag cgccggagcg
     1261 acgcgaagcc gcgcgggatc gacccaggtg gacgccaatg gcaacgtctc cattggcggc
     1321 agcagccgca cgagctacac ggccggcctc gacgcacgct gggagatcga cctgtgggga
     1381 cgcatccggc gcctcgtcga agccgcgcgc gcgggagagc aggcgagcgc cgcggacctc
     1441 gaggcggcga agctctcgat ccgggccgag ctcgccacga actatttcca gctgcgcgtg
     1501 accgacgtca cgcgcgatct cctcgacgac acggtcaagg cgttccagtc ctcgtacgac
     1561 gtgacgcaga accgctaccg ggcgggcgtc gccggaaaag tcgacgtcgt gcaggccgag
     1621 gcgcagctgc tgagcaccca ggcgca
//
LOCUS       DASVII010000745         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_178396,
            whole genome shotgun sequence.
ACCESSION   DASVII010000745 DASVII010000000
VERSION     DASVII010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178396"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..900)
                     /locus_tag="VF309_09730"
     CDS             complement(247..900)
                     /locus_tag="VF309_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466910.1"
                     /translation="MVALSSMIVPLVAPKGGRVHRGAGWVFVIAMTGVSATAFALAIR
                     RFTLADAPHPNAHAIGLFLFYVATLTAAGVSSGVRALRFKARSAPHRGAWDVGISAAN
                     VAMALATLVYGVIDQRTLLIAFSLVGLMTGTGQLRYWLRTPTTRHHWWFAHMGGMLAS
                     CVAALTALVVVNAARFGSRTFSTTIWIAVPALGIPAIFAWTAYYRRRFATRPPAELP"
     gene            1066..>1591
                     /locus_tag="VF309_09735"
     CDS             1066..>1591
                     /locus_tag="VF309_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466911.1"
                     /translation="MRAAGFGTWLVALLPSTVAFLRSELAGPRAIGFAIGAITFLAAY
                     SAACRARAGRRNAPLAALAAQSLAAVAMVWFGRGVVTAAIFVVVASQLPGVVSGGVAT
                     AWVALQSATIAVRFDALHTGPALASCAAFAGFQAFALAAASLAASERRAGAAAAAHSR
                     TIERLRVARDLHDTL"
BASE COUNT          221 a          540 c          595 g          235 t
ORIGIN      
        1 ggtgtcaccg gcatagacgt agtgcttgat ggcgtcgaag aagtacggtc tcgttccttc
       61 ccgcgatgca ccgccatcga gcgtcgcgct cgcgagggcc ggctgctcgg ccgccattcg
      121 cgcgaccgcc gtgccttcgt cggcaacgat cgcttgcatc gccaccaaca gccacgtatc
      181 cggtatggcg tgccttacgc gttgccttac cggacgcgtt cctcctcggc cggccaccgc
      241 gtgccgtcac ggcagctccg ccggcggacg ggtggcgaag cggcgacggt agtacgccgt
      301 ccaggcgaag atcgccggaa tgccgagggc ggggacggcg atccagatcg tggtcgagaa
      361 ggtacggctg ccaaaccgcg cggcgttgac gacgacgagc gcggtgagcg cggcgacgca
      421 ggaggcgagc atgccgccca tgtgcgcgaa ccaccagtgg tggcgcgtgg tgggcgttcg
      481 cagccagtag cggagctgac cggtgccggt catcaagccc acgagcgaga acgcgatgag
      541 cagcgtgcgc tgatcgatca cgccgtacac cagcgtcgcc agcgccatcg cgacgttggc
      601 cgcggaaatc ccgacgtccc acgccccgcg atgcggcgcg ctgcgcgcct tgaagcggag
      661 cgcgcgcacg ccggacgaca cgcccgccgc ggtcagcgtg gccacgtaga agaggaacaa
      721 gccgatcgcg tgggcattgg ggtgcggcgc gtctgccagc gtgaagcgcc ggatggcgag
      781 cgcgaacgcg gtggccgaca cgcccgtcat cgcgatcacg aacacccagc cggcgccgcg
      841 atgcacgcgg ccccctttgg gtgcgacgag cgggacgatc atcgacgaca gcgcaaccac
      901 gccggcgatg acgtgtggcg tgagcaagag atcgtgcatg gcgggatgat ggtaggttgg
      961 cgccgggcgc gcatgtgccg gaggtcatgc gatcgaccat gacttctggc acgggcgtcg
     1021 cggtggccag atcggtatgc ttgccgcgtg cggccggagg cctggatgcg agcggcgggg
     1081 ttcggcacgt ggcttgtcgc cttgttgccg agcacggtgg catttttgcg ctccgagctc
     1141 gcggggccgc gcgcgatcgg gttcgcgatc ggggccatca cgttcctcgc ggcgtacagt
     1201 gcggcgtgcc gcgcgcgggc cggccggcgc aatgcaccgc ttgcggcgct cgcagcacag
     1261 tcgctcgcgg cggttgccat ggtgtggttc gggcgcggcg tcgtgaccgc ggcgatcttc
     1321 gtcgtggtcg cgagccaact gccgggcgtc gtgtcgggcg gcgtcgcgac cgcttgggtc
     1381 gcgctgcagt cggcaacgat cgcggtgcgc ttcgatgcgc tgcacaccgg ccctgcgctc
     1441 gccagctgcg cggcgttcgc cggcttccag gcgttcgcgc tcgccgcggc ctcgctggcc
     1501 gcgagcgagc ggcgtgcggg cgccgccgcc gccgcgcact cccgcacgat cgagcgcttg
     1561 cgcgtcgcgc gcgacctcca cgacacgctc g
//
LOCUS       DASVII010000746         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_322195,
            whole genome shotgun sequence.
ACCESSION   DASVII010000746 DASVII010000000
VERSION     DASVII010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322195"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>1866
                     /locus_tag="VF309_09740"
     CDS             12..>1866
                     /locus_tag="VF309_09740"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEX7466912.1"
                     /translation="MATNREFHARSLKDPEGFWAEEARRIDWHKPFAKVLEYTNPPFR
                     KWFVGGETNLCYNAVDRHVKERGDQAALVWISTEVHQTRTFTYAELYAEVNRFAAVLR
                     DLGVGKGDRVLIYMPMTPEAVFAMLATVRLGAIHSVVFGGFAAASLAARIDDARPKVM
                     VTSDCGMRMGKVIALKPLVDESMRLAKYPPQHVLIADRGIDQGMKLTQGRDVDYAQER
                     EKQRGVEVPVTWLESNEPSYILYTSGTTGKPKGVQRDTGGYAVALAASMRHIFCSNPG
                     EAYFCTSDIGWVVGHSYIVYGPLINGSTTIMYEGVPMRPDAGIWWKIVQDYKVTSMFS
                     SPTAIRVLKKQDPAWMKKYDVSSLRYLYLAGEPLDEPTARWVAESLGAQIVDNYWQTE
                     SGWPILTACPGIEDTPRKFGSPSFAAYGYNLKLKHEETGKEVAANEKGVLCIVPPLPP
                     GAMSTIWGDDARYVSTYFKSFADEMVYTTFDWATRDADGYYFVLGRTDDVINVAGHRL
                     GTREIEEAVQAHSNVAEVAVVGVADSLKGQVPMAFAVVKDSARIATPELAAAHEKEIM
                     ATVDKELGAIGRPSRVCFVTALPKTRSGKLLRRSIQAICEGRDPGDLTTIED"
BASE COUNT          330 a          610 c          635 g          291 t
ORIGIN      
        1 tcgctagccc gatggccacg aaccgggaat tccacgcgcg ttcgttgaag gatccggagg
       61 gcttctgggc cgaggaggcg cggcgcatcg attggcacaa gccgttcgcc aaggtcctcg
      121 agtacaccaa ccctcccttc cgcaagtggt tcgtgggcgg cgagaccaat ctttgctaca
      181 acgcggtcga ccgccacgtg aaggagcgcg gcgaccaggc tgcgctcgtg tggatctcga
      241 ccgaggttca ccagacgcgc acgttcacct acgccgagct ctatgccgag gtgaaccgct
      301 tcgccgcggt gctgcgcgac ctcggcgtgg ggaagggcga tcgcgtgctg atctacatgc
      361 cgatgacgcc cgaggcggtc ttcgcgatgc tcgcgaccgt gcgcctgggg gcgatccact
      421 cggtggtgtt cggcggattc gccgcggcga gcctcgccgc ccgcatcgac gatgcgcggc
      481 cgaaggtgat ggtgacgagc gactgcggaa tgcgcatggg caaggtgatc gccctgaagc
      541 ccctcgtcga cgagtcgatg cgcctggcga aatacccgcc gcagcatgtc ctcatcgccg
      601 atcgcggcat cgaccagggg atgaagctca cgcaggggcg cgacgtcgac tacgcgcagg
      661 agcgcgagaa gcagcgcggt gtggaagttc cggtcacgtg gctcgaatcg aacgagccct
      721 cgtacatcct ctacaccagc ggcaccaccg gaaagcccaa gggcgtgcag cgcgacacgg
      781 gaggctatgc ggtcgcgctc gccgcctcga tgcgccacat cttctgctcc aacccgggcg
      841 aagcgtattt ctgcacctcg gacatcggct gggtggtggg ccattcgtac atcgtctacg
      901 gcccgctcat caacggctcg accacgatca tgtacgaggg cgtgccgatg cgccccgacg
      961 cgggcatctg gtggaagatc gtgcaggact acaaggtgac gtcgatgttc tcgtcgccta
     1021 ccgcgatccg cgtcctcaag aagcaggacc cggcgtggat gaagaagtac gacgtgtcgt
     1081 cgctgcgcta cctctacctc gcgggcgagc ccctcgacga gccgacggcg cgctgggtcg
     1141 cggaatccct cggcgcgcag atcgtcgaca actactggca gacggaatcg ggctggccga
     1201 tcctcacggc gtgccccggc atcgaagata cgccgcgcaa gttcggctcg ccgtcgttcg
     1261 cggcctacgg ctacaacctg aagctcaagc acgaggaaac cggcaaggaa gtcgcggcca
     1321 acgagaaggg cgtgctgtgc atcgtgccgc cgctgccgcc gggagcgatg tcgacgatct
     1381 ggggcgacga cgcgcgctat gtgagcacgt atttcaagtc gttcgccgac gagatggtct
     1441 acacgacctt cgactgggcg acccgcgacg ccgacggcta ctacttcgtg ctcggccgca
     1501 ccgacgacgt gatcaacgtc gcgggccatc ggctgggcac gcgcgagatc gaggaggccg
     1561 tgcaggcgca ttcgaacgtg gccgaggtcg cggtcgtggg cgtcgccgac tccctaaagg
     1621 gccaggtgcc catggcgttc gcggtcgtga aggactcggc gcggatcgcc acgcccgagc
     1681 tcgcggcggc gcacgagaag gagatcatgg cgaccgtcga caaggagctc ggcgccatcg
     1741 ggcgtccctc gagggtgtgc ttcgtgacgg cgctcccgaa gacgcgatcg ggaaagctct
     1801 tgcgccgctc gatccaggcg atctgcgagg ggcgcgatcc cggcgacctc accaccatcg
     1861 aggacc
//
LOCUS       DASVII010000747         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_214649,
            whole genome shotgun sequence.
ACCESSION   DASVII010000747 DASVII010000000
VERSION     DASVII010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214649"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VF309_09745"
     CDS             <1..547
                     /locus_tag="VF309_09745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7466913.1"
                     /translation="ERLQPGDVSDIMRSPNGFHIVKLLEKRGKAAATGVQQTHVRHIL
                     VRAREGLSENEARDRLQRLRQRIAAGEDFADLAKNNSEDASAAKGGDLGWVAPGDTVP
                     EFERVMNALKDQEVSPPIQTPFGWHLVQVLERRSDELSGDRKKLAARQAIRARKADEA
                     YQDWLRQSRDRAFVENRYDER"
     gene            549..1514
                     /gene="pdxA"
                     /locus_tag="VF309_09750"
     CDS             549..1514
                     /gene="pdxA"
                     /locus_tag="VF309_09750"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7466914.1"
                     /translation="MPLVALTAGEPAGIGPDLCAILAREKFPGRVVIIGDPDVIAERA
                     RMRGIAFDIPAYRDRESAPAVSILPVAAAAPVTPGRLDAANARHVLAILDRAIDGCVT
                     DEFDAMVTAPVQKSVINEAGVPFTGHTEYLAERTGTPLVVMLLAGGGLRVALATTHLA
                     LSEVPRAITRERIEAVIRVLDADLRKRFGIMRPRILVAGLNPHAGESGYLGREEIDSI
                     APAIEALAREGFAVSGPYPADTLFTPRVLEQGDCALAMFHDQGLPVLKHAAFGKAVNV
                     TLGLPIIRTSVDHGTALDRAGAGEIDAGSFHEAIALAFEMASFSR"
     gene            1522..>2082
                     /gene="rsmA"
                     /locus_tag="VF309_09755"
     CDS             1522..>2082
                     /gene="rsmA"
                     /locus_tag="VF309_09755"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7466915.1"
                     /translation="MLPRAKKRFGQHFLTDKHYLARIADAIAPRESDSMVEIGPGTGI
                     LTEALAPRLAHLHAIEIDRDLAADLAARFPAGKLTVHRADALDFDFAALPAPLRVVGN
                     LPYNVSTPILFRIAAIAERLQDAVFMLQKEVVERMVAAPDTEAYGRLSVMLQYRFEMA
                     LLFRVPPGAFTPPPKVDSAVVLMKPLP"
BASE COUNT          314 a          774 c          699 g          295 t
ORIGIN      
        1 cgagcgcctg cagcccggcg acgtgagcga catcatgagg agccccaacg gcttccacat
       61 cgtgaagctc ctcgagaagc gcggcaaggc cgcagccacc ggcgtgcagc agacgcacgt
      121 gcgccacatc ctcgtgcgcg cgcgcgaagg cctgtcggag aacgaggcgc gcgaccgcct
      181 gcagcgcctg cgccagcgca tcgcggccgg cgaggatttc gccgatctcg ccaagaacaa
      241 ctccgaggac gcgagcgccg cgaagggcgg cgatctcgga tgggtagcgc cgggcgacac
      301 cgttcccgag ttcgagcgcg tcatgaacgc gttgaaggac caggaggtga gcccgccgat
      361 ccagacgccc ttcggctggc acctggtgca ggtcctcgag cgccgatccg acgagctctc
      421 cggcgacagg aagaagctcg ccgcgcgcca ggcgatccgc gcacgcaagg ccgacgaggc
      481 gtaccaggac tggctgcgcc agtcgcgcga ccgcgccttc gtcgagaacc gctacgacga
      541 gcgctagcct gccgctggtc gcgctcactg ccggcgagcc cgccggcatc ggccccgacc
      601 tgtgcgcgat cctcgcgcgc gagaagtttc ccgggcgcgt ggtgatcatc ggcgaccccg
      661 acgtgatcgc cgagcgcgcg cgcatgcgcg gcatcgcctt cgacatcccc gcgtatcgcg
      721 atcgcgagag cgcgcccgcg gtgtcgatcc tccccgtggc cgccgccgcg ccggtgacgc
      781 ccggacgcct ggatgccgcc aacgcgcgcc acgtcctcgc gatcctcgat cgcgccatcg
      841 acggctgcgt caccgacgaa ttcgacgcga tggtcacggc gccggtgcag aagagcgtca
      901 tcaacgaggc gggcgtgccc ttcaccggcc acaccgagta cctcgccgag cgcaccggca
      961 cgccgctggt ggtgatgctg ctggcgggcg gcgggctgcg cgtggcgctc gccaccacgc
     1021 acctcgcgct ctccgaggtg ccgcgcgcca tcacgcgcga gcggatcgaa gcggtgatcc
     1081 gcgtcctgga cgcggacctg cgcaagcgct tcggcatcat gcgcccgcgc atcctcgtgg
     1141 cggggttgaa tccgcacgcc ggcgagtcgg gctacctggg gcgcgaggag atcgacagca
     1201 tagcgccggc gatcgaagcc ctcgcgcgtg aaggcttcgc cgtgtcgggc ccttatcccg
     1261 ccgatacgct tttcacgccg cgcgtcctcg agcagggcga ctgcgcgctc gcgatgttcc
     1321 acgaccaggg cctgccggtg ctgaagcacg ccgcattcgg caaggcggtg aacgtgacgc
     1381 tgggattgcc gatcatccgc acctccgtgg accacggcac cgcgctcgac cgcgccggcg
     1441 cgggcgagat cgacgccggc agcttccacg aggccatcgc gctcgccttc gagatggcgt
     1501 ctttcagccg ctaggcccgc gatgctgccg cgtgccaaga agcgcttcgg ccagcatttc
     1561 ctcaccgaca agcattacct cgcgcgcatc gccgatgcga tcgcgccgcg ggaatccgac
     1621 tcgatggtgg agatcgggcc cggcaccggc atcctcaccg aggcgctcgc gcccaggctc
     1681 gcgcacctgc acgcgatcga gatcgatcgc gacctcgcgg cggatctcgc ggcgcgcttt
     1741 cccgcgggca agctcaccgt ccaccgcgcc gacgcgctcg acttcgattt cgccgcgctc
     1801 cccgcgccgc tgcgcgtggt gggcaacctg ccctacaacg tctccacgcc gatcctgttt
     1861 cgcatcgccg ccatcgccga gcgcctccag gacgcggtct tcatgctgca gaaggaagtg
     1921 gtcgagcgca tggtcgccgc gccggacacc gaggcctacg ggcggctttc ggtgatgctg
     1981 cagtaccgct tcgagatggc gctcctgttt cgcgtgccgc cgggcgcctt cacaccgcca
     2041 ccgaaggtgg attccgcggt ggtgctgatg aagccgctcc ca
//
LOCUS       DASVII010000748         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_214695,
            whole genome shotgun sequence.
ACCESSION   DASVII010000748 DASVII010000000
VERSION     DASVII010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214695"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /gene="xseB"
                     /locus_tag="VF309_09760"
     CDS             complement(<1..188)
                     /gene="xseB"
                     /locus_tag="VF309_09760"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7466916.1"
                     /translation="MAAKSQGISPSELSFEKALEELEALVARMEDGKLPLEESLAAYQ
                     RGAELVKFCESKLTDAQAR"
     gene            373..1485
                     /locus_tag="VF309_09765"
     CDS             373..1485
                     /locus_tag="VF309_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7466917.1"
                     /translation="MSDLASQRLLTRTSLHLPLSWYFDPKVADIERKLLFAQGPGYVA
                     HEQMAREPGDYRVLDWRSQGAWSLVNNGGRHDLISNVCRHRQAIMLQGAGSLPGGTIT
                     CPLHRWSYAGDGTHLGAPEFPDHPCLDLPRQSLQKWNGMLFNGKRDVARDLANLGVAK
                     ELDFADYRLDKVETVEYDFNWKTFVEVYLEDYHVGPFHPGLGHFVTCDDLHWELGDWH
                     SVQTVGVNQRLGTPGSPTYKAWHDQVLKYSGGEPPPHGAIWLTYYPNVMVEWYPNVLV
                     ISTLHPRGPEKCVNVIEFYYPEEIALFEPDYMAAQQKAYHETALEDEEICLRMTRGRK
                     ALLEDGREEHGPYQSPMEDGMVHFHEFIRREIGQHV"
BASE COUNT          321 a          552 c          543 g          345 t
ORIGIN      
        1 cgcgcctggg catcggtcag cttcgattcg cagaacttga cgagctccgc gccgcgttgg
       61 taggcggcaa gcgactcctc gaggggaagc ttgccgtctt ccatgcgcgc gacgagggcc
      121 tcgagctcct cgagggcttt ctcgaagctc aattcggaag gggaaatgcc ctgggatttc
      181 gctgccatcg ataggtagcc tcgtgtgtcg ccttgcgcac attagcctgc aaggaatttt
      241 acaccaaagg tcttgaaaat taagggaact cgccccaaaa tagcagtggg acggagctgc
      301 agagttaaaa gctagctgac tcgatttgcc cgcatgttgt tttcatcttc gtttgtactg
      361 ggggtgcgga taatgtcgga tctcgcgagt cagagactgc tcacgcgcac gtctcttcac
      421 ctgccgcttt cgtggtactt cgacccgaag gtggcggaca tcgagcggaa gctccttttc
      481 gcccagggcc ccgggtatgt ggcgcacgag cagatggcgc gcgagcccgg cgactatcgc
      541 gtgctcgact ggcgctccca gggcgcgtgg tcgctcgtga acaacggcgg gcgccacgat
      601 ctcatctcca acgtgtgccg ccatcgccag gcgatcatgc tgcagggcgc gggctcgctt
      661 cccggcggca ccatcacctg cccgctgcat cgctggagct acgcgggcga cggcacgcac
      721 ctcggcgcgc ccgagttccc cgatcaccca tgcctcgacc tgccgcggca atcgctgcag
      781 aagtggaacg gcatgctctt caacggcaag cgcgacgtgg cgcgcgatct tgcgaatctc
      841 ggcgtcgcga aagagctcga cttcgccgac taccggctcg acaaggtcga gaccgtcgaa
      901 tacgacttca actggaagac cttcgtcgag gtctatctcg aggactatca tgtcggcccg
      961 ttccatccgg ggctcgggca tttcgtcacg tgcgacgacc tgcattggga gctcggcgac
     1021 tggcacagcg tgcagacggt cggcgtgaac cagcgccttg gcacgcccgg ctcgcccacg
     1081 tacaaggcgt ggcacgatca ggtgctcaag tacagcggcg gcgagccacc gccccacggc
     1141 gcgatctggc tcacctatta cccgaacgtg atggtcgagt ggtatccgaa cgtgctcgtc
     1201 atctccacgc tccatccgcg cggtcccgag aagtgcgtga acgtgatcga gttctattac
     1261 cccgaagaga tcgcgctatt cgagcccgac tacatggccg cgcagcagaa ggcgtaccac
     1321 gagaccgcgc tcgaggacga ggagatctgc cttcgcatga cgcgcgggcg caaggcgctg
     1381 ctggaagatg ggcgcgagga gcacgggccg tatcaatcgc cgatggaaga cgggatggtg
     1441 cacttccacg agttcatccg ccgggagatc gggcagcacg tctagccgtc ttttgtatcc
     1501 tctaaatttg aatttcagag gatacggtct gctgcaaaca agacggggcg gcccaagccg
     1561 ccccgtttcg ctcttttgct cctcctcctt attcgttgtc gaactcgtgg accacggccc
     1621 acagggtcgc gaaggtgtgc tgcgaataat cggcgtgcaa ctcttcgtaa tcctcggcgc
     1681 gaacgggctc ttcctccttg cccttgcgga tctcgaacca gcgctgctgg atcttctcgc
     1741 gcgtcgcgtg gggcggcatc g
//
LOCUS       DASVII010000749         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_287182,
            whole genome shotgun sequence.
ACCESSION   DASVII010000749 DASVII010000000
VERSION     DASVII010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287182"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF309_09770"
     CDS             <1..257
                     /locus_tag="VF309_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7466918.1"
                     /translation="ERLRNTSPVEMAARIKAPVFMAYGGADVRVVPEHGTRMKAALEK
                     AGQHPQWMLADGEGHGYREMKNEVMFYSAMEKFLAENIGK"
     gene            complement(277..1176)
                     /locus_tag="VF309_09775"
     CDS             complement(277..1176)
                     /locus_tag="VF309_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7466919.1"
                     /translation="MKPVSSAPTWALLAGAAMWGVLWYPYRLLAHAGLDGIWSSLVTY
                     GVALVAGIAIFPRVAWSLRRIPAAAIVMGAAIGWSNLAYVIAVLEGEVMRVLLLFYLA
                     PLWTVPIARVLLKEKANAAGLAVMALAFAGAMVMLWKPQIGLPWPQARAEWLGLAAGF
                     GFAVGNVLVRRLQGMGDVAKSMVIWAGVTVACGVHLSWSRIPAADAAHVTLANAALVA
                     GIAVVLVVMSLALQYGLSRMPANRAIVILLFELVVAAFAAWLLAGETMRVQDWIGGAL
                     IVAATLVSELKTGDRPQLSAGDN"
     gene            1193..>1658
                     /locus_tag="VF309_09780"
     CDS             1193..>1658
                     /locus_tag="VF309_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7466920.1"
                     /translation="MSSCAVLHITSLLGGGVDRHVRDIARAAPGRHVVWHTSDSADVI
                     EVSGTGELLPLDGAAIERDPAPLAEWLRAMGVGIVHAHSVGRAARRRAAWAAQALGVP
                     TIVTLHDILFLRREGFEPGAARGADPAWLALTAPFLRAAAAVIAPSRFVADLA"
BASE COUNT          290 a          598 c          554 g          216 t
ORIGIN      
        1 aggaacggct gcgcaacacg tcgccggtgg agatggccgc gcgcatcaag gcgccggtgt
       61 tcatggcgta cggcggcgcc gacgtgcgtg tggtccccga gcacggcact cgcatgaagg
      121 ccgcgctcga gaaagccggc cagcacccgc aatggatgct cgccgacggc gagggacacg
      181 gctatcgcga aatgaagaac gaggtcatgt tctacagcgc gatggagaaa ttccttgccg
      241 agaacatcgg caagtaaccg gaaccgggga cagacctcaa ttatcgcctg ctgacaattg
      301 aggtctgtcc ccggttttca gctcgctcac cagcgtggcg gcgacgatga gcgcgccgcc
      361 gatccagtcc tgcacgcgca tggtctcgcc ggcgaggagc cacgcggcga aggcggcgac
      421 caccagctcg aagagcagga tcacgatggc gcggttggcg ggcatgcgcg acagcccgta
      481 ttgcagcgcg aggctcatca ccaccagcac cacggcaatg ccggcgacga gagccgcgtt
      541 ggcgagagtc acgtgcgcgg catcggcggc ggggatgcgg ctccatgaga ggtgcacgcc
      601 gcaggctacc gtcacgcccg cccagatcac catcgacttc gccacatcgc ccatcccctg
      661 caggcggcgc accagcacgt tgcccaccgc gaagccgaat cccgcggcaa gccccagcca
      721 ctcggcgcgc gcctgcggcc acggcagccc gatctgcggc ttccacagca tcaccatcgc
      781 gcccgcgaaa gcgagcgcca tcaccgccag ccccgcggcg ttcgccttct ccttcagcag
      841 cacgcgcgcg atcggcaccg tccagagcgg cgcgaggtag aagagcagca gcacgcgcat
      901 cacctcgcct tcgagcaccg cgatcacgta cgcgagattg ctccagccga tcgccgcgcc
      961 catcacgatc gccgccgcgg gaatgcggcg caggctccac gcgacacgcg gaaagatcgc
     1021 gatgccggcg acgagtgcca cgccgtaggt cacgagcgac gaccagatcc cgtcgaggcc
     1081 cgcgtgcgcg aggaggcgat acggatacca gagcacgccc cacatcgcgg ccccggccag
     1141 cagcgcccag gtcggcgcgg aagaaacggg cttcaaggta ccatccaaaa agatgagctc
     1201 gtgcgcagtt ttacacatca cctcgcttct cggcggcggc gtggaccggc atgtgcgcga
     1261 catcgcccgc gcagcgccgg gccgccatgt cgtctggcat acatccgaca gcgccgacgt
     1321 gatcgaggtg tcggggacgg gggagctcct gccgctcgac ggggccgcga tcgagcgcga
     1381 tcccgcgccc cttgccgaat ggctacgcgc aatgggtgtc gggattgtgc acgcgcactc
     1441 ggtggggcgc gcggcgcgcc ggcgtgcggc gtgggcagcg caggcgctcg gcgtcccgac
     1501 catcgtcacg ctgcacgaca tcctcttcct gcgccgcgaa ggcttcgagc cgggcgcggc
     1561 gcgcggcgcc gatcccgcat ggctcgcgct caccgcgccg tttctgcgcg ccgcggcggc
     1621 cgtgatcgcg ccttcccgtt tcgtcgccga cctcgcgc
//
LOCUS       DASVII010000750         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_682538,
            whole genome shotgun sequence.
ACCESSION   DASVII010000750 DASVII010000000
VERSION     DASVII010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682538"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /gene="argB"
                     /locus_tag="VF309_09785"
     CDS             <1..724
                     /gene="argB"
                     /locus_tag="VF309_09785"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7466921.1"
                     /translation="VVHGAGPQIGALLQKLGKQSEFIQGMRVTDEETLDVVEMVLGGL
                     INQDIVTLINKAGGKAVGLTGKDGHFIHARKLKIPSKENKKKKIDVGLVGEIVRIDPE
                     IIQLLDQRDFIPVIAPIGSDENGTAYNINADVAAGKLAITLQAEKLVLLTNTTGVLDK
                     EGKVITGLNAQKVKEMIDDGTITGGMLPKVDCALDAVRNGVKSAHIIDGRVSHALLLE
                     VLTSSGVGTLIRGAAANRGNSR"
     gene            complement(736..1488)
                     /locus_tag="VF309_09790"
     CDS             complement(736..1488)
                     /locus_tag="VF309_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7466922.1"
                     /translation="MPRAQTLSVVTYNIHKGLSQFNKRLVLHEIGDRLKSLDADVAFL
                     QEVQGKHDANARRHVTWPATGQSEFLTQDGCHSVYGMNAVYQDGHHGNAVVSRHPIPV
                     WENIDVSHHAMESRGLLHCEVHIPGWKEPVHCINVHLGLWARSRRYQLEWLSERIRAA
                     VPKKGPLIVAGDFNDWHRKASDYLWDELGLVEVFEKAEGRPARSFPAKMPVFTLDRIY
                     VRDLNVESVQRHTGAQWSRLSDHVALAARLSR"
     gene            complement(1491..>1728)
                     /gene="nudB"
                     /locus_tag="VF309_09795"
     CDS             complement(1491..>1728)
                     /gene="nudB"
                     /locus_tag="VF309_09795"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX7466923.1"
                     /translation="NLRNVYEIYPVWRHRYAPGITHNTEHVFGLELPEPLAPKLAPRE
                     HLAAMWLPWAEAAERVFSWSNRKAILMLPERKRA"
BASE COUNT          312 a          551 c          583 g          282 t
ORIGIN      
        1 ggtggttcat ggtgcggggc cccagatcgg tgcgctgctg cagaagctcg gcaagcagag
       61 cgagttcatc cagggcatgc gcgtgaccga cgaggagacc ctcgacgtcg tcgagatggt
      121 gctggggggc ctcatcaacc aggacattgt cacgctcatc aacaaggcgg gcggcaaggc
      181 cgtcggtctc accggcaagg acggccactt catccacgcg cgcaagctca agatcccgag
      241 caaggaaaac aagaagaaga agatcgacgt gggcctggtc ggcgagatcg tgcgcatcga
      301 cccggagatc atccagctcc tcgaccagcg cgacttcatc ccggtgatcg cccccatcgg
      361 aagcgacgag aacgggaccg cctacaacat caacgccgac gtggccgcgg gcaagctcgc
      421 gatcacgctc caggccgaga agctggtgct gctcaccaac acgacgggcg tgctcgacaa
      481 ggaaggcaag gtcatcaccg gcctgaacgc gcagaaggtg aaggagatga tcgacgacgg
      541 caccatcacc gggggcatgc tgccgaaggt cgactgcgcc ctggacgcgg tgaggaacgg
      601 cgtgaagagc gcgcacatca tcgacgggcg cgtctcccac gcgctgctcc tcgaagtgct
      661 cacgagctcg ggcgtcggca ccttgatccg cggggctgcg gcgaatcgcg gcaacagtcg
      721 ctgacgattt cgtcgctagc gggaaagccg cgccgcgagc gcgacgtggt ccgaaaggcg
      781 cgaccattgg gcgccggtgt ggcgctgcac gctctccacg ttgaggtcgc gcacgtagat
      841 gcggtcgagc gtgaagaccg gcatcttcgc cgggaagctg cgcgccgggc ggccttcggc
      901 cttctcgaac acttcgacca ggccgagctc gtcccagagg taatcgctcg ccttgcggtg
      961 ccagtcgttg aagtcgccgg cgacgatcag cgggcccttc ttcggaacgg cggcgcggat
     1021 gcgctccgag agccattcga gctggtagcg ccggctgcgc gcccacaatc ccaggtgcac
     1081 gttgatgcaa tgcacgggct ccttccatcc ggggatgtgg acctcgcaat gcagcaggcc
     1141 gcggctctcc atggcatggt gcgagacgtc gatgttctcc cacacgggaa tcgggtggcg
     1201 gctcacgacc gcgttgccgt ggtggccatc ctggtacacg gcattcatgc cgtagaccga
     1261 atggcacccg tcttgcgtga ggaactcgct ctgccccgtc gcgggccacg tgacgtggcg
     1321 gcgcgcgttg gcgtcgtgct ttccctggac ctcctgcagg aatgcgacgt ccgcgtcgag
     1381 cgacttcagc cggtcgccga tctcgtgcag aacgagacgc ttgttgaact gcgacaggcc
     1441 cttgtggatg ttgtaggtca cgaccgaaag cgtctgcgcg cgcggcatcg ctaggctcgc
     1501 tttctttcgg gaagcatgag gatcgccttt cggttgctcc aggaaaacac tcgctccgcg
     1561 gcctcggccc acggcagcca catcgccgcg aggtgctccc tgggcgcgag cttcggcgcc
     1621 aggggctcgg gaagctcgag gccgaagaca tgctcggtat tgtgcgtgat cccgggcgcg
     1681 tagcggtggc gccacaccgg atagatctcg tacacgttcc gcaggttc
//
LOCUS       DASVII010000751         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_538736,
            whole genome shotgun sequence.
ACCESSION   DASVII010000751 DASVII010000000
VERSION     DASVII010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538736"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1685)
                     /locus_tag="VF309_09800"
     CDS             complement(69..1685)
                     /locus_tag="VF309_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7466924.1"
                     /translation="MQRLPPKPYERGGDDPASSSALKSPTPTGQQPLPALQNATLKLG
                     RTRRPGSINLERIAIAGATGYIGGRLASRLLSAGYALRCLARSPRKLEGREWASDSRV
                     EIRKADLADQTALTDELRGCEAAFYLVHSMMSADREYARRDLDLALTFARSALDAGVR
                     RIIYLGGLGETGFGLSEHLSSRRDVESALGSAGVPVTVLRAAMIIGSGSASFEILRYL
                     VQRLPVMITPKWVTTRCQPIAVENVLNYLVGVLSAPETTGGVFDIGGSETLCYRDIMR
                     IMAEELGLPRRWIIAVPVLTPRLSSYWIHLVTPLSNKIARPLAEGLKNEVVCREDRIT
                     RIIPQKLLNVREAIHAALSQVESHLVETNWTMAGAMPGDPDWSGGTVFRDEREVGIAA
                     PAGAVFRAVCRLGGGHGWYADWLWKIRGGLDRLAGGPGLRRGRRDPDTLRYGDALDFW
                     RVVGLDRDRSLSLRAEMRLPGQALLDFRIGANGARNCTLRQTALFEPRGLFGLMYWYA
                     VLPLHGLVFRGMLAGIQRDALRLAALEPAG"
BASE COUNT          374 a          583 c          545 g          334 t
ORIGIN      
        1 actcggcata tcgacgggtt gcgcgttatc cgtcaatgtg gttgcttccg ttgcgacgcc
       61 gattccgact atcccgctgg ctccagagcc gccagtcgga gagcgtcacg ctgaatgcct
      121 gcgagcatac cgcggaagac gaggccatga agcggcagca cggcgtacca gtacattaat
      181 ccgaacaacc cccgcggctc aaagagggcg gtctgtcgca gcgtgcagtt ccgggcgcca
      241 ttcgctccga tcctgaaatc gagcagtgcc tggccgggca gacgcatttc tgcccgcagg
      301 gaaagggagc gatccctgtc caaaccgacc actcgccaga aatcgagagc gtcgccgtac
      361 ctgagcgtgt cgggatcccg ccggccacgg cgcagccctg ggccgcctgc gagtcgatcc
      421 agcccgccgc gaatcttcca tagccagtcc gcataccacc catgtccgcc gcctaagcga
      481 catacagcac gaaacacggc tcctgcgggg gcggcaatac ctacttcgcg ctcatcgcga
      541 aacactgtgc ctcccgacca gtccggatcg cctggcatgg cacccgccat cgtccagttc
      601 gtttccacca ggtgcgactc cacctggctc agggcggcat ggatcgcctc acgcacgttc
      661 agcagtttct gcggaatgat ccgcgtgatc cgatcctcgc gacagactac ttcgtttttc
      721 aacccctcgg ccagcggtct ggcgattttg ttgctcagag gcgtcaccag gtgaatccaa
      781 tacgaactca gacggggggt gaggaccggc accgcgatga tccaacggcg cggaagtccg
      841 agctcctcag ccatgatgcg catgatgtcg cggtagcaaa gtgtctcact cccgccgatg
      901 tcaaaaacac caccggtagt ttccggggcc gacaagacgc caaccaggta attgagcaca
      961 ttctcgacgg cgatgggctg gcagcgggtt gtgacccact ttggcgtgat catcactggc
     1021 aaacgttgaa ccagatagcg aagaatctcg aaagacgccg aacccgagcc aatgatcatc
     1081 gctgcgcgca ggactgtaac cggcactccg gcagaaccga gcgccgactc cacatcccgg
     1141 cgcgaagaga gatgttcgct aagaccgaaa cccgtctctc caagcccgcc cagataaatg
     1201 atgcggcgca ctcccgcgtc gagcgcggat ctcgcgaatg tcaaagcaag atcgaggtcc
     1261 cgccgtgcat actcgcggtc tgccgacatc atcgagtgca ccagatagaa tgccgcttca
     1321 catccgcgga gttcatccgt gagcgctgtt tggtcggcga gatcggcttt ccggatctcc
     1381 actcgggaat ctgaggccca ctctcgaccc tctaacttgc gcggggatcg cgcgaggcag
     1441 cggagcgcgt aaccggcact gagaaggcga gacgccagcc ggcctccgat atagcccgtg
     1501 gctcctgcga tcgcaatgcg ctccaaattg atgctccctg ggcggcgggt gcggcccagc
     1561 ttgagtgtag cgttttggag ggcaggcagt ggttgctgcc cggttggagt gggactcttc
     1621 agcgcggagg aagatgcagg gtcgtcgcct cctctctcat atggcttcgg tggaaggcgt
     1681 tgcatccgga tgaagacatc gcggacagaa tctgagacgg acctcagcgc gatgggcgtt
     1741 agacggcaat ttgaccctga ggtcaccgat ctcgccgaat ggtgagtaag gcggaaatcg
     1801 tttgattctg ttcctccggt gcaacccctc cgtacg
//
LOCUS       DASVII010000752         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_646622,
            whole genome shotgun sequence.
ACCESSION   DASVII010000752 DASVII010000000
VERSION     DASVII010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646622"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2059)
                     /locus_tag="VF309_09805"
     CDS             complement(80..2059)
                     /locus_tag="VF309_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7466925.1"
                     /translation="MPLDTVVVSATRGETRAFDAPAAIGSVDGATIRAAGPMVNLSEA
                     LDRVPGVSVLNRQNYAQDLQLSIRGFGSRSTFGIRGVRLIVDGIPATMPDGQGQASNV
                     SLGSAARIEVLRGPLALLYGNAAGGVVQVFTEAGAREPTLTATASSGSFGLRREEVMF
                     AATEGAHSATLDASHFATSGYRAHSTASRDQINGKWTWQVSGATRLDTVVNVLDQDAL
                     DPLGLTRAQWQQDPRQSPAIAFTQDARKSVSQKQAGTVIEHRFDDDTTLGARVYAGKR
                     DLDNKLSTPFAAQLPPSSSGGIVSFDRVYSGAGLQLARRIAIADSIVARVLAAVEIDR
                     MRDDRQGYLNNAGVQGALRRDEDDIVADTDALLQASVDFGDRWSAVAGVRSSRVRFDT
                     HDRYIAPGNPDDSGTLIYRGTNPVAGLTWHVSPELNVYSNAGRGFETPTFTEVAYRNN
                     ASGLNTDLRASHSNHAEIGAKWRPAAGHSLEAAVFDIRTRDEIVVDTNVGGRSTFRNA
                     GSTTRRGGEVSYVGRLADTLRTTVALTALEAKFDNGRHLPGTPERSAFAELVYTPRVP
                     LAGFHTAIEVVHVGRIFVDDANSDSAPAATILNLRAGFRERVGMIEIEPLLRLDNATN
                     RRYAGSVIVNEANSRFFEPAPTRNWLLAVTARYRF"
BASE COUNT          331 a          742 c          829 g          381 t
ORIGIN      
        1 tcctgtattc aagagtgcgc cttgtggggc gggtcaaacc cgccccgttt tttttgttcg
       61 ccgcgaagag cctcgccgtt cagaaccggt agcgcgcggt cacggcgagg agccagttgc
      121 gcgtgggcgc gggctcgaag aagcggctgt tcgcctcgtt cacgatcacc gagcccgcgt
      181 agcgtcggtt ggtcgcgttg tcgaggcgca gcagcggctc gatctcgatc atcccgacgc
      241 gctcgcgaaa gccggcgcgc aggttgagaa tcgtggccgc gggcgcggag tcgctgttcg
      301 cgtcgtcgac gaagatcctt ccgacgtgga ccacctcgat tgccgtgtgg aagcccgcga
      361 ggggcacgcg cggcgtgtag acgagctcgg cgaatgcgct gcgctcggga gtgccgggaa
      421 ggtggcggcc attatcgaac ttcgcctcga gcgcggtgag cgcgaccgtc gttcgcagcg
      481 tatccgccag gcgtcccacg tacgagacct cccctccgcg ccgcgtcgtg ctgccggcgt
      541 tgcggaaggt ggagcgcccg ccgacgttgg tgtccaccac gatctcgtcg cgcgtgcgga
      601 tgtcgaagac cgcggcttcg agcgagtggc ccgcggccgg gcgccacttg gcgccgatct
      661 ccgcgtggtt gctgtgggag gcgcgcaggt ccgtgttgag gcccgacgcg ttgttgcgat
      721 aggcgacttc ggtgaaggtg ggcgtctcga atccgcgtcc ggcgttcgag tagacgttca
      781 attcgggcga cacgtgccag gtgaggcccg cgacgggatt ggtcccgcga tagatgaggg
      841 tgccgctgtc gtccgggttt cccggcgcga tgtagcgatc gtgagtgtcg aagcgcacgc
      901 ggctcgagcg aacgccggcc accgcgctcc agcgatcgcc gaaatcgacg ctcgcctgca
      961 gcagcgcgtc ggtgtcggcg acgatgtcgt cctcgtcacg gcgcaacgcg ccctgtacgc
     1021 ccgcgttgtt gaggtagccc tggcgatcgt cgcgcatgcg gtcgatctcg actgcggcga
     1081 ggacgcgcgc gacgatcgaa tcggcgatgg cgatgcgccg cgcgagctgc aggcccgctc
     1141 ccgaatagac ccggtcgaag ctcacgatgc cgccgctcga gctcggtgga agctgcgccg
     1201 cgaagggcgt cgagagcttg ttgtcgaggt cgcgcttgcc ggcgtagacg cgcgcgccga
     1261 gggtggtatc gtcgtcgaag cggtgctcga tgaccgtgcc cgcctgcttc tggctcacgc
     1321 tcttgcgggc atcctgcgtg aacgcgatcg ccggcgattg ccgcggatcc tgctgccact
     1381 gggcgcgcgt gagcccgagc ggatcgagcg cgtcctggtc gaggacgttc accaccgtat
     1441 cgagccgcgt ggcgccgctc acctgccaag tccacttgcc gttgatctga tcgcgcgagg
     1501 ccgtgctgtg cgcgcgatag ccgctggtcg cgaagtgcga cgcgtcgagc gtcgcgctat
     1561 gggcgccctc ggtcgccgcg aacatgacct cctcgcggcg caagccgaac gatccggagg
     1621 atgccgtcgc ggtgagcgtg ggttcgcgcg cgcccgcttc ggtgaacacc tgcaccacgc
     1681 ctccggccgc gttgccgtag agaagggcca ggggtccgcg cagcacctcg atgcgtgcag
     1741 ccgacccgag gctcacgttg gaagcctggc cctggccgtc cggcatcgtc gccggaatgc
     1801 cgtcgacgat gaggcgcacg ccgcgaattc cgaatgtgga gcgcgagccg aatccgcgga
     1861 tcgagagctg caggtcctgc gcgtagttct ggcgattgag gacgctcacg ccgggcacgc
     1921 ggtcgagcgc ctccgacagg ttcaccatcg ggcccgccgc gcgaatcgtg gcgccgtcga
     1981 cgctgccgat cgcggcgggc gcatcgaatg cgcgggtctc gccgcgcgtc gcggacacga
     2041 ccaccgtgtc gagaggcacc ggcggcgcct cctgcgcgta cgacgacgaa caggcgaggg
     2101 ccgcggcaag gaaggcgcag tcgttcttca gggcgtgtct ctcgcctgga tgagaatcaa
     2161 agcgtaccac tctcgttcgg caacgaccgc ttgtgggaaa tgcgccgggg ggtctaggat
     2221 cgtcaagcgc cgagtcggag tcgcttcgcg gccaaaagct gtccttggcg gcaaacggcg
     2281 gat
//
LOCUS       DASVII010000753         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_682644,
            whole genome shotgun sequence.
ACCESSION   DASVII010000753 DASVII010000000
VERSION     DASVII010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682644"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1201)
                     /locus_tag="VF309_09810"
     CDS             complement(14..1201)
                     /locus_tag="VF309_09810"
                     /inference="COORDINATES: protein motif:HMM:NF005601.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7466926.1"
                     /translation="MNDTTSRIHIAARMAHIEPFEVMEIQTLAREIEAQGRDVIHLEI
                     GEPDFTTPRPIVEAAQRALESKPMFYTSALGLAELRKAISKFYRDRYHVDVPASRIIV
                     TAGSSAALLLALGVLLDPEDEVLLTDPSYPCNRHFVHTLGGVPRLIPVGPESRYQLNA
                     QIARRHWTAKTRVAMVATPSNPTGTMVTPEEIAELVALTREKGATLLVDEIYHGLTYG
                     LDARTALEAGDDIFVINSFSKYFQMTGWRLGWLVAPPQYVREIEKLAQNLFISPSAVA
                     QHAALACFDERTIAIVEKRRAELDQRRRYLIPALESLGFRIPVIPQGAFYIYADSSPL
                     AADSFVLARRILAEAGVAVTPGKDFGHHEPERHIRIAYTQPVARLEEAVARIGALLGG
                     KRS"
     gene            complement(1220..>1785)
                     /locus_tag="VF309_09815"
     CDS             complement(1220..>1785)
                     /locus_tag="VF309_09815"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX7466927.1"
                     /translation="LIEAIVDRAFIGEDALPRSAKAFEDQKKRAKARLPAVTEGASRY
                     ATAVGEASQQFLQSLAQHASLGRVAQEAKGQRERLVYPGFLARTPWERLEHMPRYLKG
                     YALRLEKYRGNPERDQKHAANVQTLWNNYEARVKADRDEGRRDPNLEEFRWLLEELRI
                     SLFAQELRTPLPVSAKRLQKFWDEHLR"
BASE COUNT          284 a          568 c          610 g          323 t
ORIGIN      
        1 caacggtcga ggatcatgat cgtttccctc cgagaagagc gccgatgcgc gctaccgctt
       61 cctccagccg cgccacgggc tgcgtgtacg cgatgcgaat gtggcgctcg ggctcatggt
      121 gcccgaagtc cttccccggc gtgacggcga cgcccgcctc cgcgaggatg cgccgcgcca
      181 ggacgaagct gtcggcggcg agcggcgagg aatcggcgta gatgtagaac gctccctgtg
      241 gaatcaccgg gattctgaaa ccgagggatt cgagcgccgg gatgagatag cggcgacgct
      301 gatcgagctc ggcgcgacgc ttctcgacga tcgcgatggt acgctcgtcg aagcaagcga
      361 gtgccgcatg ctgcgcgacg gccgagggcg agatgaacag gttctgcgcg agcttctcga
      421 tctcgcgcac gtattgcggc ggcgccacta gccagccgag ccgccatccg gtcatctgga
      481 agtatttcga gaagctgttg atgacgaaga tgtcgtcgcc ggcttcgagc gcggtgcgcg
      541 catcgagccc gtaggtgagg ccgtgataga tctcgtccac gaggagagtc gcgcccttct
      601 cccgcgtgag cgccacgagc tccgcgatct cttccggcgt caccatcgtc cccgtgggat
      661 tggagggcgt cgcgaccatg gcgactcgcg tcttcgcggt ccagtggcgg cgcgcgatct
      721 gcgcgttcaa ctgatagcgg ctctccggcc cgacgggaat gagacgcggc acgccgccca
      781 gcgtgtgcac gaaatgacgg ttgcatggat agctcggatc ggtgaggagc acttcgtcct
      841 ccggatcgag gaggacgccg agcgcgagaa gcaatgccgc gctcgagccc gcggtcacga
      901 tgatgcggct cgccggaacg tcgacgtggt accgatcgcg gtagaacttc gagatcgcct
      961 tccgcagctc ggcaagtccc agcgccgacg tgtagaacat cggcttcgat tccagcgcgc
     1021 gctgcgccgc ctcgacgatg ggccgcggcg ttgtgaagtc cggctcgccg atctcgaggt
     1081 ggatcacgtc gcggccctgc gcctcgatct cgcgggcgag ggtctggatc tccatcacct
     1141 cgaagggctc gatgtgagcc atccgggcag caatgtggat gcgactggtc gtgtcgttca
     1201 tcgttcgatt ctaatgcgcc tagcgcaagt gctcgtccca gaatttctgc agccgcttcg
     1261 ccgaaacggg aagcggcgtg cgcagctcct gcgcgaagag cgagatgcgc agctcctcga
     1321 ggagccagcg gaactcttcg aggttgggat cgcgccgccc ttcgtcgcgg tcggccttca
     1381 cgcgcgcctc gtagttattc cagagcgtct ggacgttcgc ggcgtgcttc tggtcgcgct
     1441 cgggattgcc gcggtacttc tccaatctca gcgcgtagcc cttgagatat cgcggcatgt
     1501 gctcgaggcg ctcccacggc gtgcgcgcga ggaagcccgg atacaccagg cgctcgcgct
     1561 ggcctttcgc ctcctgcgcc acgcggccga gggaggcgtg ctgcgcgagc gactgcagga
     1621 attgctggct cgcctcgccg accgccgtgg cgtagcgtga tgcgccttcg gtcaccgcgg
     1681 gaaggcgcgc cttggcgcgc ttcttctgat cctcgaaggc ctttgccgag cgtgggagcg
     1741 catcctcgcc gatgaatgcg cgatcgacga tggcttcgat gagat
//
LOCUS       DASVII010000754         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_682646,
            whole genome shotgun sequence.
ACCESSION   DASVII010000754 DASVII010000000
VERSION     DASVII010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682646"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF309_09820"
     CDS             <1..265
                     /locus_tag="VF309_09820"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HEX7466928.1"
                     /translation="EGFLPGGASLHNAMSAHGPDRASYEAAIAVQLKPAHLERTLAVM
                     FESRYLFEPTAHALASPTLDKDYDSVWSGFEKAALAPMAEEKK"
     gene            262..1284
                     /locus_tag="VF309_09825"
     CDS             262..1284
                     /locus_tag="VF309_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7466929.1"
                     /translation="MKLASLKGPTRDGTLVVVDRALARAVKADGIAPTMQHLLENWDA
                     LAPKLRDVAASLEGGHERHAFDFRQVLTRGEVAAPLPRAYEWLDGSAYLSHVERVRRA
                     RNDKVPESFYKDPLMYQGGAGAMMGPRDPIRALSEDWGIDLEGEVVALVGDVPMGATP
                     AQAAAAVRLLTLVNDVSFRRLIPAELAKGFGFVNGKGANALAPVAATPDELGDRWKDG
                     RIHLPMTCDVNGKQLGNPDAGADAQFGLHELIAHAAKTRDLAAGTMVGTGTISNHDLS
                     AGFACLMEARLVEQVETGAAKTPFLQFGDSVRIEMRDASGASVFGAIEQQVISASAGI
                     QPEPRG"
     gene            1386..>1686
                     /locus_tag="VF309_09830"
     CDS             1386..>1686
                     /locus_tag="VF309_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466930.1"
                     /translation="MWRRLYARQAALIPDYACDEFRDALRDLDFGAAIPRFDAINAKL
                     EPATDWQLVAVPGLLPDLTFFDHLAHRRFPVTVWLREPAEFDYIVEPDIFHDFFGH"
BASE COUNT          272 a          584 c          568 g          262 t
ORIGIN      
        1 ggaaggtttc ctgccgggtg gtgcgagcct gcacaacgcg atgagcgccc acgggcccga
       61 tcgcgcgtct tacgaggccg cgatcgccgt gcaattgaag cccgctcacc tcgagcgcac
      121 gctcgccgtc atgttcgaga gccgctatct cttcgagccg acggcgcacg cgctcgcgtc
      181 gcccacgctc gacaaggact acgacagcgt ctggtcgggc ttcgagaagg cggcgctcgc
      241 ccccatggca gaggagaaga agtgaagctc gcctcgctga aaggccccac ccgcgacggc
      301 acgctcgtcg tcgtcgatcg cgcgctcgcc cgcgccgtga aggccgacgg catcgcgccc
      361 accatgcagc acctgctcga gaactgggac gcgctcgcgc cgaagctgcg cgacgtcgcg
      421 gcgagcctcg aaggagggca cgagcgtcac gcgttcgatt tccgccaggt actgacgcgc
      481 ggcgaggtcg cggcgccatt gccgcgcgct tacgaatggc tcgacggttc ggcgtatctg
      541 tcgcacgtcg agcgcgtgcg ccgcgcgcgc aacgacaagg tccccgagag cttctacaag
      601 gatccgctca tgtaccaggg cggcgcgggc gccatgatgg gaccgcgcga tccgatccgc
      661 gcgctttccg aggactgggg catcgatctc gaaggcgaag tggtcgcgct cgtgggcgac
      721 gttcccatgg gcgccacgcc cgcgcaggcc gctgcggcgg tgcggcttct cacgctggtg
      781 aacgacgtct cgttccgtcg attgattccg gccgagctcg cgaagggctt cggtttcgtg
      841 aacggaaagg gcgcgaatgc gctggcgccg gtcgcggcca cgcccgacga gctgggagat
      901 cgctggaagg acgggcgcat ccacttgccg atgacgtgcg acgtgaacgg caagcagctc
      961 ggcaatcccg acgcgggcgc cgacgcgcag ttcggattgc acgagctcat cgcgcacgcg
     1021 gcgaagactc gcgatctcgc ggcgggcacg atggtcggca ccggcaccat ctccaaccac
     1081 gatctttccg cgggcttcgc gtgcctcatg gaagcgcgcc tggtcgagca ggtcgagacg
     1141 ggcgccgcga agacgccctt cctgcagttc ggcgactcgg tgcgcatcga gatgcgcgac
     1201 gcctcgggcg cgagcgtctt cggcgcgatc gagcagcagg tcatttccgc gagcgcggga
     1261 atccagccag aaccccgggg atgaccggcg agacccagca caacctgcgc ggcgactaca
     1321 gccgcatgcg gccggactac acggtcgagc aggagtggct gggctattcg cccgagtagc
     1381 acgaattgtg gcggcgtctc tatgcccgcc aggccgcgct cattcccgac tatgcgtgcg
     1441 atgaattccg cgacgcgctc cgggatctcg acttcggagc cgccatcccg cgcttcgatg
     1501 cgatcaacgc gaagctcgag cccgcgaccg actggcagct cgtcgcggtt ccggggctcc
     1561 tgcccgatct cacgttcttc gaccatctcg cccatcgccg cttcccggtg acggtgtggc
     1621 tgcgcgagcc ggcggaattc gactacatcg tcgagcccga catcttccac gacttcttcg
     1681 gccacg
//
LOCUS       DASVII010000755         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_178892,
            whole genome shotgun sequence.
ACCESSION   DASVII010000755 DASVII010000000
VERSION     DASVII010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178892"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..627
                     /locus_tag="VF309_09835"
     CDS             331..627
                     /locus_tag="VF309_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7466931.1"
                     /translation="MARGHSYLWTPWLVIGEIASNALIAIALLVIGLRLVRRVRAGDG
                     EGLARAAAWTGVLAFALASTHLLDVWVTWTPIYAIDLAVRGVVALVAVAAMFLI"
     gene            630..>1796
                     /locus_tag="VF309_09840"
     CDS             630..>1796
                     /locus_tag="VF309_09840"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX7466932.1"
                     /translation="MPFSIIPGRNVPIHAWAPVQEIEPQALAQLEKIAALPWVVHHVA
                     VMPDVHLGKGATVGSVVAMRDAVAPAAVGVDIGCGMAAVETTLAAADLPDNLHGLRAE
                     IERAIPVGMSSHDAPAWAAAPAETRATAKTLLGGFDALEGGVQQLGERASCQLGTLGG
                     GNHFIEICLDAAGTVWLMLHSGSRHIGNALAEHHMQIARKLQHNQALEDRDLAVFLAR
                     TPEFAAYRRDLFWAQEYARCNREVMLHLLKEIVRKLWPKVGFREAISCHHNYVAEERH
                     FGEELLVTRKGAIRAGAGDLGIIPGSMGTKSYIVRGKGSAESFDSASHGAGRRMSRTQ
                     ARKRFSVGDLREQTEGVECRKDPGVIDEAPKAYKNIDRVMAEQRDLVEIVAELKQ"
BASE COUNT          288 a          623 c          606 g          279 t
ORIGIN      
        1 cggacgagga ggcgctgccg ccctcgatat gcgtccgcct gacgatcccc tgcgcccctc
       61 tgtcttcccg tgcgcgcacc atggcccgag tcggacaacg gcaaggcggc gaaaaactta
      121 actcttccgt cattaaatgc acgttggctt gcacttttga accatggact taacgctcac
      181 ttcggagaat cgccgcgagc ggccggccca tcctccggag acatccagcg aacatcacat
      241 cgaaatccgc tcaatctttg ccacggacca ccggcctcgg catgctttgc ggcggtgccc
      301 gtgcgcgcaa tgttctcgac ggagatcttc atggcccgcg ggcactcgta cctgtggacg
      361 ccgtggctgg tcatcggtga gatcgcgtcg aacgccctga tcgcgatcgc cctcctcgtg
      421 atcggcctcc gcctggtccg ccgcgtccgc gcgggcgacg gcgaggggct tgcgcgtgca
      481 gcggcatgga ccggcgtcct ggccttcgcc ctggcctcga cgcacctcct cgacgtgtgg
      541 gtcacctgga cgccgatcta cgccatcgac ctggcggtcc ggggcgtggt cgcgctcgtc
      601 gccgtggccg caatgttctt gatctgacca tgccgttctc catcatcccg gggcgcaacg
      661 ttcccatcca cgcctgggcg cccgtgcagg agatcgagcc ccaggcgctc gcccagctcg
      721 agaagatcgc agccctaccc tgggtcgttc accacgtcgc ggtcatgccc gacgttcacc
      781 tcggcaaggg cgcgacggtc ggatcggtgg tcgcgatgcg ggacgcggtc gcgccggccg
      841 ccgtgggcgt cgacatcggc tgcggaatgg cggcggtcga aaccacactc gcggcggccg
      901 acctgcccga caacctgcac gggctgcgag cggagatcga gcgcgcgatc ccggtcggga
      961 tgtcgtcgca cgacgcgccc gcctgggccg cggcgcccgc ggagacgagg gcgaccgcga
     1021 agacgctgct cggcggcttc gacgcgctcg aggggggcgt gcaacagctc ggcgagcgag
     1081 cctcctgcca gctcggaacg ctgggcggcg gcaaccattt catcgagatc tgcctggatg
     1141 cggccggcac cgtctggctg atgctccact cggggagccg ccacatcggc aacgcgctcg
     1201 ccgagcacca catgcaaatc gcgcgcaagc tccagcacaa ccaggccctg gaggaccggg
     1261 atctcgccgt cttcctggcg cggacgcccg agttcgccgc ctaccggcgg gatctgttct
     1321 gggcgcagga gtacgcgcgc tgcaaccgcg aggtgatgct gcatctgctg aaggaaatcg
     1381 tgcgaaagct ctggccgaag gtcgggttcc gcgaggccat ctcctgccac cacaactacg
     1441 tcgccgagga gcgccacttc ggcgaggagc tgctggtcac gcgcaagggg gcgatccgcg
     1501 ccggcgccgg agacctcggc atcatccccg gttcgatggg gaccaagtcg tacatcgtgc
     1561 gcggcaaggg gagcgccgag tccttcgatt cagcgtcgca cggcgcgggg cgacgcatga
     1621 gccggaccca ggcgcgcaag cggttctccg tgggcgatct gcgcgagcag accgaggggg
     1681 tcgagtgccg caaggacccc ggcgtcatcg atgaggcgcc gaaggcctac aagaacatcg
     1741 atcgcgtgat ggccgagcag cgggacctgg tggagatcgt cgccgagctc aaacag
//
LOCUS       DASVII010000756         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_503128,
            whole genome shotgun sequence.
ACCESSION   DASVII010000756 DASVII010000000
VERSION     DASVII010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503128"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF309_09845"
     CDS             <1..560
                     /locus_tag="VF309_09845"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7466933.1"
                     /translation="SRPASRKKSTAIEDLRAIPWVFSWAQCRVMLPGWYGFGAAVTAF
                     LARRGAPGEALLERMWREWPFFRAMLSNLDMLLAKADMSVAARYKELLADRELAEKIF
                     GRIHGEFQATVKALFGITGSTGFLAANPALARSIRNRFPYLDPLNHLQLELLKRHRAG
                     DDEERVKRGIHLSINGLAAGLRNSG"
     gene            complement(557..1189)
                     /locus_tag="VF309_09850"
     CDS             complement(557..1189)
                     /locus_tag="VF309_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX7466934.1"
                     /translation="MITVYHLSTSRSERIVWLMEELGLEYRLETFQREPSGAAPPPMK
                     ALHALGKAPVIRDGDVVLAESAAIVDYIVHRHAGGRLAVPPEAPEFAGYLYWLHFAEG
                     SLMSLLLIALVLSRVPEANASPVKARIHERVKQMLAFVDASLAGHPYFAGERFTAADV
                     MMAFPFTTLRHFLELDLSPYANIAAYLERIESRPAYRKAMELAGPRDKYR"
     gene            complement(1302..1439)
                     /locus_tag="VF309_09855"
     CDS             complement(1302..1439)
                     /locus_tag="VF309_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX7466935.1"
                     /translation="MKALLAVIIGASFLLAACNTMEGAGKDVSAVGKKTTEEAQEHKK
                     Y"
BASE COUNT          270 a          538 c          557 g          281 t
ORIGIN      
        1 gttcgcgtcc cgcctcgcgc aagaaatcca cggcgatcga ggacctgcgg gcgatcccgt
       61 gggtgttctc atgggcgcaa tgccgcgtga tgttgcccgg atggtatgga ttcggcgccg
      121 cggtgacggc gttcctcgcg cggcgcggcg cgccgggcga ggcgctcctc gagcgcatgt
      181 ggcgcgaatg gccgttcttc cgcgccatgc tctcgaatct cgacatgctg ctcgcgaagg
      241 ccgacatgtc cgtcgccgcg cgatacaagg agctgcttgc cgaccgcgaa ctggcggaga
      301 agatcttcgg gcgcatccac ggcgaattcc aggccacggt gaaagctctc ttcggcatca
      361 cggggagcac cggcttcctc gcggcgaatc cggcgctggc gcgctcgatc cgcaaccggt
      421 ttccttacct cgaccccctg aaccacctgc agctcgagct cctcaagcgc caccgcgctg
      481 gcgacgacga ggagcgcgtg aagcgcggga ttcacctgtc gatcaacggg ctcgccgcgg
      541 ggcttcgcaa ctcgggctag cggtacttgt cgcgcgggcc ggcgagctcc atcgccttcc
      601 ggtacgcggg gcgcgactcg atccgctcga ggtaggccgc gatgttcgcg taaggcgaca
      661 ggtccaattc gaggaagtgg cgcagggtcg tgaacggaaa cgccatcatg acgtcggccg
      721 cggtgaagcg ctcgcccgcg aagtaggggt gcccggcgag cgacgcatcg acgaaggcca
      781 gcatctgctt cacgcgctcg tgtatccgcg ccttgacggg gcttgcgttc gcttcgggca
      841 cacgcgacag caccagcgcg atcagcagga gcgacatgag gctcccctcg gcgaagtgca
      901 gccagtagag gtagcccgcg aattcgggcg cttcgggggg caccgcaagg cgcccgccgg
      961 catgacgatg gacgatgtag tcgacgatcg cggcggattc ggcaagcacc acgtcgccgt
     1021 cccggatgac gggtgccttg ccgagcgcgt ggagcgcctt catcggcggc ggcgcggcgc
     1081 cgctgggctc gcgctggaac gtttcgagcc tgtattcgag accgagctct tccatgagcc
     1141 acacgatccg ctcggaacgc gaggtcgaga gatggtagac ggtgatcacg gcgctccctt
     1201 cgtcgaaggc gcgcctattc tgcccatttg gccggcggga tgctcaggcc tcctcgaggc
     1261 ccaggcacgt cgcccggacc atcgggcgat acggacggcg ctcagtactt cttgtgctcc
     1321 tgggcttcct ccgtcgtctt cttgccgacg gccgaaacgt ccttgcccgc gccttccatg
     1381 gtgttgcagg ccgcgagaag gaacgaggcg ccgatgatca ctgcgagcaa tgctttcatg
     1441 tccgtgttct ccgtgaatgg cccgcgctgc gaaccgtccc gaaggctagc gggagccggg
     1501 cgtcgcgtct gttcgccagc gcacctaacg gcgcgctgca gcaccaacgg atctaaataa
     1561 ctataagcct aggtaaaggc cttccgaggg tccatgctgg tgtcgtcgcg tcgacaaaca
     1621 cgagggattc cggtgcaagg gcaggc
//
LOCUS       DASVII010000757         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_610999,
            whole genome shotgun sequence.
ACCESSION   DASVII010000757 DASVII010000000
VERSION     DASVII010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610999"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..764)
                     /locus_tag="VF309_09860"
     CDS             complement(30..764)
                     /locus_tag="VF309_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7466936.1"
                     /translation="MHERLLELDNVTAGYAQDIDVLREVSLAVRAGEVSGLIGLNGAG
                     KSTVVKTICGFLAPKSGRIELDGQSIAGIEPHRLIDRGICCIPQESSLFPYLSVEENL
                     LLPLQGRAKRFAGAIDARYEEALEIFPALKERRRAQAGDLSGGQQKQVEFAKAWLQRP
                     RLCLIDEPSIGLAPILAEGVFAWIEKFAQSGMGILLIDHNVRRVVRMSDTINVLSLGR
                     ITASGGREDFQGDLHEQVRQWLGLDF"
     gene            complement(757..1479)
                     /locus_tag="VF309_09865"
     CDS             complement(757..1479)
                     /locus_tag="VF309_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7466937.1"
                     /translation="MSLLATSGLTKHFGGVAAVEGLALSMEEGEVLGLIGPNGSGKTT
                     ALNMLSGFLVPDAGRVWIAGEEVTGMKPVALARRGMLRMFQMTRVFPRISAFDNLITA
                     GLARGLSEKEAQSRAGAMLEELTLTAVQYLDAGQLSGGQRKLLEFGMCFIAPPRVALL
                     DEPFAAVHPVMRETMAGFIRRRNAEGHAFLVVSHDMPIVVDLCARTACMNAGRLIAVG
                     PTREVLKESAVIEAYLGSGSHA"
     gene            complement(1476..>2207)
                     /locus_tag="VF309_09870"
     CDS             complement(1476..>2207)
                     /locus_tag="VF309_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7466938.1"
                     /translation="APAAFAMLGAYVTALLDYHWKAPLAIGIPAAIAGTALLGWLLGR
                     IVLRLKGPYLALTTLSFAEIARVVVGNSHEFTRGDQGLNVPTLMQSRVGYYYLFLAVL
                     LAVLFGLHRLMRGRAGRFWLAIRDDETGAESRGIDVVRYKTLAFALSCAICGLAGSLY
                     GTFSQLVSPELGLLLQTGLVISMVVIGGMGTLTGPIVGALLVYLSSEWLRGVGNIQLI
                     VFSLVVIVFARFLRTGLWGLATRRR"
BASE COUNT          379 a          775 c          744 g          309 t
ORIGIN      
        1 aaacactagc ctcggccggc tgcaaaagtt cagaagtcca ggccgagcca ttggcgaacc
       61 tgctcgtgca ggtcgccctg gaagtcctcg cgcccgccgg aggcggtgat gcgcccgagg
      121 ctcagcacgt tgatcgtgtc ggacatgcgc accacgcggc gcacgttgtg gtcgatcagc
      181 aggatgccca tgccggactg cgcgaacttc tcgatccagg cgaacacgcc ctcggcgagg
      241 atcggcgcga ggccgatgct gggctcgtcg atcaggcaca ggcgcgggcg ctgcagccac
      301 gccttcgcga actccacctg cttctgctgc ccgcccgaga ggtcgccggc ctgcgcgcgc
      361 cgcctttcct tcaacgcggg aaagatctcg agggcctcct cgtagcgcgc gtcgatcgcg
      421 ccggcgaagc gcttcgcccg tccctgcagc ggcaggagga ggttttcctc cacgctcagg
      481 tagggaaaga ggctcgactc ctgcgggatg cagcagatgc cgcggtcgat gaggcgatgg
      541 ggctcgatgc cggcgatcga ttgcccgtcg agctcgatgc gccccgattt cggcgcgagg
      601 aatccgcaga tcgtcttcac cacggtcgac ttgcccgcgc cgttcaatcc gatgagcccg
      661 ctcacctcgc ccgcgcgaac cgcgagcgac acctcgcgaa ggacgtcgat gtcctgcgcg
      721 tagcccgcgg tcacgttgtc gagctcgaga aggcgctcat gcatggctgc cgctcccgag
      781 ataggcctcg atcaccgcgc tctccttgag cacctcgcgg gtcgggccca cggcgatgag
      841 ccgccccgcg ttcatgcacg ccgtgcgcgc gcacaggtcg accacgatcg gcatgtcgtg
      901 gctcaccacg aggaacgcgt ggccctcggc attgcgccgc cggatgaagc ccgccatggt
      961 ctcgcgcatc accgggtgca ccgcggcgaa cggctcgtcg aggagcgcca cgcgcggcgg
     1021 cgcgatgaag cacatgccga actcgagaag cttcctctgc ccgcccgaca gctgccccgc
     1081 gtcgagatac tggaccgccg tgagcgtgag ctcctcgagc atcgcgcccg cgcgcgactg
     1141 cgcttccttt tcggagaggc cgcgcgcgag gcccgccgtg atcaaattat cgaacgccga
     1201 gatgcgcggg aagacgcgcg tcatctggaa catgcgcagc atcccacggc gcgcgagggc
     1261 gacgggcttc atgccggtga cttcctcccc ggcgatccat acgcggccgg catcgggcac
     1321 gaggaatccc gacagcatgt tgagcgccgt ggtcttgccg gagccgttcg ggccgatcag
     1381 gcccaagacc tcgccctcct ccatcgacag cgcgagcccc tcgaccgcgg cgactccgcc
     1441 gaagtgcttg gtgaggccgg aggtagcgag gaggctcatc ggcggcgcgt ggcaaggccc
     1501 cagagcccgg tgcgcaagaa gcgcgcgaag acgatcacca ccagcgagaa cacgatcagc
     1561 tggatgttgc ccacgccccg cagccactcc gaggagagat acaccagcag cgcgccgacg
     1621 atcggcccgg tgagcgtgcc catgccgccg atcaccacca tcgagatcac cagtccggtc
     1681 tgaaggagca atccgagctc ggggctcacc agctggctga aggtgccgta gagggagccg
     1741 gcgagcccgc agatcgcgca cgacagcgcg aaggcgaggg tcttgtagcg caccacgtcg
     1801 atgccgcggc tctcggcgcc ggtttcgtcg tcgcggatcg cgagccagaa gcgccccgcg
     1861 cgcccgcgca tgagccggtg cagcccgaac agcaccgcca gcagcacggc gagaaagaga
     1921 tagtagtagc ccacccggct ttgcatcagc gtcggcacgt tgaggccctg gtcgccgcgc
     1981 gtgaactcgt gcgagttgcc gacgacgacg cgcgcgatct ccgcgaacga gagcgtggtg
     2041 agcgcgaggt acgggccctt cagccgcagc acgatcctgc cgagcagcca gccgaggagc
     2101 gcggtcccgg cgatcgccgc gggaatgccg atcgcgaggg gtgccttcca gtggtagtcg
     2161 aggagcgcgg tcacgtacgc cccgagcatc gcgaaagccg ccggcgc
//
LOCUS       DASVII010000758         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_503160,
            whole genome shotgun sequence.
ACCESSION   DASVII010000758 DASVII010000000
VERSION     DASVII010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503160"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..>2499)
                     /locus_tag="VF309_09875"
     CDS             complement(106..>2499)
                     /locus_tag="VF309_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7466939.1"
                     /translation="AAAEYRPIVVAYRNGAAVRLGDLGEVLDSVQDVRNYGMANGKPA
                     VLMIINRQPNANIIEVVDSITELLPELRASIPASIDLQVMLERTSTIRASLHEVERTL
                     MISVALVILVVFVFLRNGRATLIPAVAVPISLIGTFAAMYILGFSLNNLSLMALTIAT
                     GFVVDDAIVVLENVSRHIEKGMEPFEAALLGAREVAFTVVSMSVSLVAVFIPILFMGG
                     IVGRLFREFAVTLSVSIGISLVVSLTTTPMMCSLLLRRADERKPGRIARFSEAAFDKL
                     HRGYERSLGWALRFPPLMLFVFFATLVLTGLLYYYIPKGFFPQQDTGRLIGSVRADQS
                     ISFQAMKEKLNRLAAIVIADPAVESVTAFTGGGQRNSAQMFIALKPLKQRKDSLDQIL
                     ARLRPALSKEPGANLFLNPIQDLRGGGRQSDASQQFTLRADDLSELRTWAPKLEAALR
                     DVPELTDVSSDQQDRGLQMTLVIDRPTAARMGITPRAIDTALNLAFGQAVVSTIYQDR
                     NQYRVVMEAAPEYTQGPQTLDQIFVLSPARGQVPLSAFSHYEPTLAPLSVNHQGQFAA
                     ATISFNLAEGVAMSEASAAIRDTMARIGVPSTIYASFEGTAKLFQQGLDNQPYLIIAA
                     LLTVYIVLGILYESVVHPITILSTLPSAGVGALLALRLFNVEFSLIALIGVILLIGIV
                     KKNAILMIDFALETERKGNRDPKDAIYEAALLRFRPIMMTTMAAMLGAIPLALGIGDG
                     AELRRPLGISIVGGLLVSQLLTLYTTPVIYLYLDRFRLWARGKRRSPSVDLAAQAGD"
BASE COUNT          442 a          769 c          898 g          390 t
ORIGIN      
        1 caagcgcgaa acgtcccttg gagacctcga acagtgcttg ttcaagatcg aagtcccgaa
       61 tgtggttttg cggcgttcat cggcgtttat cggcggattc aaggctcagt ctccagcctg
      121 cgcagcgaga tcgacggaag gcgagcggcg tttgccgcgc gcccagaggc ggaagcgatc
      181 gaggtagaga tagatcacgg gagtcgtgta gagcgtgagg agctggctca cgagcaggcc
      241 ccccacgatc gagatgccga ggggccggcg cagctccgcg ccgtcgccga tgcccagcgc
      301 gagcggaatc gcgccgagca tcgcggccat ggtcgtcatc atgatcggcc ggaagcgcag
      361 gagcgccgcc tcgtagatcg cgtccttcgg atcgcgattg cccttgcgct cggtctcgag
      421 cgcgaagtcg atcatgagga tcgcgttctt cttcacgatg ccgatgagga ggatcacgcc
      481 gatgagcgcg atgagggaga actccacatt gaagagcctc agcgcgagga gcgcccccac
      541 gccggcggag ggcagcgtcg agagaattgt tatcgggtgc accacgctct cgtagaggat
      601 gccgagcacg atgtagacgg tgaggagcgc cgcgatgatg aggtacggct ggttgtcgag
      661 cccctgctgg aagagcttgg ccgtcccctc gaacgaggcg tagatggtgc tgggaacgcc
      721 gatgcgcgcc atggtgtcgc ggatcgccgc cgaggcttcg gacatggcga cgccctcggc
      781 gaggttgaac gagatcgtcg ccgcggcgaa ctgcccctgg tggttcaccg agagcggcgc
      841 cagcgtcggc tcgtagtgcg agaaggccga cagcggaact tgtccccgcg cgggcgagag
      901 cacgaagatc tggtcgagcg tctgcgggcc ctgcgtgtac tcgggggcgg cctccatcac
      961 cacgcggtac tggttgcggt cctggtagat ggtcgagacc acggcctggc cgaacgcgag
     1021 attcaacgcc gtgtcgatcg cgcgcggggt gatgcccatg cgcgcggccg tggggcgatc
     1081 gatcacgagc gtcatctgca ggccgcggtc ctgctggtcg gagctcacgt cggtgagctc
     1141 cggcacgtcg cgcagcgccg cctcgagctt cggggcccag gtgcgcagct cgctcaggtc
     1201 gtcggcgcgc agcgtgaact gctggctcgc gtcggactgg cggccgccgc cgcgcaggtc
     1261 ctggatcggg ttgaggaaga ggttcgcgcc cggctccttc gacagcgcgg ggcgaaggcg
     1321 cgcgaggatc tggtcgaggg agtccttgcg ctgcttgagg ggcttcagcg cgatgaacat
     1381 ctgcgcggaa ttgcgctgtc cgccgccggt gaacgcggtg acgctctcca cggccggatc
     1441 cgcgatcacg atggcggcga ggcgattcaa cttctccttc atcgcctgga aggagatcga
     1501 ctggtcggcg cgcaccgagc cgatgaggcg cccggtgtcc tgctgcggga agaagccctt
     1561 cggaatgtag tagtagagca accccgtcag caccagggtc gcgaagaaga cgaagagcat
     1621 cagcggggga aaccgcagcg cccagccgag cgagcgctcg tatcccctgt gcagcttgtc
     1681 gaacgccgcc tcgctgaagc gcgcgatgcg cccgggcttg cgctcgtcgg cgcgccgcag
     1741 cagcagcgag cacatcatcg gcgtggtggt gagcgacacg acgagcgaga tgccgatcga
     1801 caccgaaagc gtcaccgcga actcgcggaa gaggcgcccg acgatcccgc ccatgaagag
     1861 gatcgggatg aacacggcga cgagcgacac gctcatggaa acgacggtga acgcgacctc
     1921 gcgggcgccc aggagcgccg cctcgaaggg ctccatcccc ttctcgatat ggcgcgagac
     1981 gttctcgagc accacgatcg cgtcgtcgac gacgaacccc gtggcgatgg tgagcgccat
     2041 gagcgagagg ttgttcaggc tgaacccgag gatgtacatc gccgcgaacg tgccgatgag
     2101 cgagatcggc acggcgaccg cgggaatcag cgtcgcgcgg ccgttgcgaa ggaagacgaa
     2161 caccacgagg atcacgagcg cgaccgagat catcagcgtg cgctcgactt cgtgcagcga
     2221 ggcgcggatg gtgctggtgc gctcgagcat cacctgcagg tcgatgctcg cgggaatgga
     2281 cgcgcgcagc tcgggcagga gctccgtgat gctgtcgacc acttcgatga tgttggcgtt
     2341 cggctggcgg ttgatgatca tcaggaccgc aggcttgccg ttcgccatgc cgtagttgcg
     2401 cacgtcctgg acgctgtcga gcacttcgcc caggtcgccc aggcgcaccg cggcgccgtt
     2461 gcggtaggcc accacgatcg gccggtactc ggcggcggc
//
LOCUS       DASVII010000759         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_143803,
            whole genome shotgun sequence.
ACCESSION   DASVII010000759 DASVII010000000
VERSION     DASVII010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143803"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VF309_09880"
     CDS             <1..554
                     /locus_tag="VF309_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7466940.1"
                     /translation="KPQVTWGTSPEMVVSIEDRVPDPSKEADPARRGAIERALEYMGL
                     EPNVAMSDISIDKVFIGSCTNSRIEDLRAAAAVVKGRRVAKNVKLALVVPGSGLVKTQ
                     AEKEGLDRVFLDSGFEWRDPGCSMCLGMNDDKLAAGERCASTSNRNFEGRQGAGGRTH
                     LVSPEMAAAAAIAGHFVDVRRGR"
     gene            554..1192
                     /gene="leuD"
                     /locus_tag="VF309_09885"
     CDS             554..1192
                     /gene="leuD"
                     /locus_tag="VF309_09885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7466941.1"
                     /translation="MEKFTVHTGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPHLFD
                     AWRYIDVGQPGMDLTKRTLNPDFVLNRPQYKGASILLARKNFGCGSSREHAPWALQQG
                     GFKAVVAPSFADIFFNNSFKNGFLPIALQEPEVARLFDEAVGAPGYRLTIDLAAQTVT
                     TPSGEAMHFDIDDFRKKSLLNGWDDIGLTLRNAAEIKAYEERRKRAEPWIFG"
     gene            1200..2261
                     /gene="leuB"
                     /locus_tag="VF309_09890"
     CDS             1200..2261
                     /gene="leuB"
                     /locus_tag="VF309_09890"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX7466942.1"
                     /translation="MKIALLAGDGIGPEILREAVRVLHVLRSEGLDVETEEALVGGAA
                     YDASGDPLPKATLDLAKSSDAVLFGAVGGPQYDTLPRDKRPEKAILGLRHECDFFANL
                     RPANVFPELADASTLKAEVVSGLDIMIVRELTGDIYFGKPRGISGPKGDRVGFNTMHY
                     TEPQIRRILHTGFKTARARGKRLCSVDKMNVLETSQLWRDIAEEIAPEYSDVALSHML
                     VDNCAMQLVRNPRQFDVIVTGNLFGDILSDEASMLTGSIGMLPSASLDARGKGLYEPI
                     HGSAPDIAGRGLANPLAMILSMAMMFRYTFGRIDIAARIEKAVRAALAAGLRTADIAA
                     KGIKPCATAEMGIAVAKAL"
     gene            2343..3479
                     /gene="asd"
                     /locus_tag="VF309_09895"
     CDS             2343..3479
                     /gene="asd"
                     /locus_tag="VF309_09895"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7466943.1"
                     /translation="MRTVGFIGWRGMVGSVLMERMREERDFDAIDPVFFTTSNVGGAG
                     PAIGKPVAPLKDANDVAELAKLDVLVSSQGGDYTKSVYPNLRSAGWGGYWIDAASALR
                     MEKDAVIILDPVNRPVIDKALQSGVKNFIGGNCTVSLMLMAIDGLLKADLVEWVSAMT
                     YQAASGAGAQNMRELLQQMGEVHFAAKSLLADPASAILDIDREVAGILRDESFPTEHF
                     GVPLAGSLLPWIDADLGNGQSREEWKGHAETNKILGREARPVAVDGLCVRVGAMRCHS
                     QALTIKMKRELPVEEIESILAKGNAWVKVVPNRREETLRRLTPVAVTGTLDVPIGRVR
                     KLRMGGEYLSAFTVGDQLLWGAAEPLRRMLGILLKARVAVGAAA"
BASE COUNT          658 a         1138 c         1200 g          596 t
ORIGIN      
        1 tcaagcccca ggtcacgtgg ggaacgtcgc ccgaaatggt cgtctcgatc gaggaccgcg
       61 tgcccgatcc gagcaaggaa gccgaccccg ccaggcgcgg cgccatcgaa cgcgcgctgg
      121 agtacatggg gctcgagccc aacgtcgcca tgagcgatat ctcgatcgac aaggtcttca
      181 tcggctcgtg caccaactcg cgcatcgagg acctgcgcgc ggccgcggcc gtggtgaaag
      241 ggcggcgcgt ggcgaagaac gtgaagctcg ccctcgtcgt gccgggatcc ggcctggtga
      301 agacgcaggc cgagaaggag ggcctcgatc gcgtgttcct cgactcggga ttcgaatggc
      361 gcgatccggg ctgctcgatg tgcctgggca tgaacgacga caagctcgcg gcgggggagc
      421 gctgcgcgtc gacctccaac cgcaacttcg agggccgcca gggtgccggc ggccgcacgc
      481 acctcgtgag ccccgagatg gcggcggccg cggcgatcgc gggccatttc gtcgacgttc
      541 gccggggacg ctgatggaga agttcaccgt ccacacggga ctcgtcgctc ctctcgaccg
      601 ggagaacgtc gataccgacg cgatcatccc caagcagttc ctgaagtcga tcaagcgcac
      661 cggcttcggc ccgcatctgt tcgatgcgtg gcgctacatc gacgtcggcc agccgggaat
      721 ggatctcacg aagagaacgc tcaacccgga tttcgtcctg aaccggcccc agtacaaggg
      781 cgcctcgatc ctcctcgcgc gcaagaactt cggctgcggc tcctcgcgcg agcatgcgcc
      841 gtgggcgttg cagcagggcg gcttcaaggc ggtcgtcgcg ccttcgttcg cggacatctt
      901 cttcaacaac tccttcaaga acgggttcct gccgatcgca ttgcaggagc ccgaggtggc
      961 gcggctgttc gacgaggccg tcggggcgcc cggctatcgc ctcacgatcg acctcgccgc
     1021 gcagacggtc accacgccct cgggcgaggc gatgcatttc gacatcgacg atttccgcaa
     1081 gaagtccctt ctcaacggct gggacgacat cggcctcacg ctgcgcaacg ccgcggagat
     1141 caaggcttac gaggagcgcc gcaagcgcgc cgaaccctgg atcttcggct gaggccggga
     1201 tgaagatcgc cttgctggcc ggagacggca tcggtcccga gatcctgcgc gaagcggtgc
     1261 gggtgctcca cgtgttgcgc tccgaaggcc tcgacgtcga gaccgaggaa gcgctggtcg
     1321 gtggcgccgc ctacgacgcg tcgggggatc cgctgccgaa ggcgacgctc gatctcgcga
     1381 agtcttcgga cgcggtgctc ttcggcgccg tcggcggccc gcaatacgac acgctcccac
     1441 gggacaagcg tccggagaag gcgatcctcg ggctgcgcca cgagtgcgat ttcttcgcca
     1501 acctgcggcc cgcgaatgtc ttccccgagc tcgcggacgc ctcgaccctg aaggcggaag
     1561 tcgtctcggg cctcgacatc atgatcgtgc gcgagctcac gggagacatc tacttcggca
     1621 agccgcgcgg gatctccggc ccgaagggcg accgtgtcgg cttcaacacg atgcactaca
     1681 ccgagccgca gatccgccgc atcctccata cgggcttcaa gacggcgcgc gcgcggggca
     1741 agcgcctctg ctcggtcgac aagatgaacg tgctcgagac ctcgcagctc tggcgcgaca
     1801 tcgccgagga gatcgccccg gagtattcgg acgtggcgct ttcgcatatg ctcgtggaca
     1861 attgcgccat gcagctggtg cgaaacccga ggcaattcga cgtgatcgtg acgggcaacc
     1921 tgttcggcga cattctttct gacgaggcat cgatgctcac gggttcgatc ggcatgctgc
     1981 cgtcggcttc gctcgatgcc cgcggcaagg gcctgtacga gcctatccac ggctcggcac
     2041 ccgacatcgc gggccggggg ctcgccaatc cgctcgcgat gatcctttcg atggcgatga
     2101 tgttccgcta tacgttcggc aggatcgaca tcgccgcgcg catcgagaag gcggttcgcg
     2161 cggcgctggc cgcggggctt cgcacggcgg acatcgcggc aaagggcatc aaaccctgcg
     2221 ccaccgccga aatgggaatc gcggtggcga aagcacttta gagccgacga gagcgcggcg
     2281 cggaagaggc ggcccgcctc tctcctaaca gcgacgcctc taccggaaag aagatcgacc
     2341 caatgagaac cgtaggattt atcggctggc gcggcatggt gggctccgtc ctgatggagc
     2401 gcatgcggga ggagcgcgat ttcgacgcga tcgatcccgt atttttcacg acctcgaacg
     2461 tcgggggcgc gggccccgcg atcggcaagc cggtcgcgcc cctcaaggac gcgaacgacg
     2521 tggccgagct cgcgaagctc gacgttctcg tgagcagcca gggcggcgac tatacgaagt
     2581 cggtctatcc gaacctgcgc tccgccggct gggggggata ttggatcgac gcggcctcgg
     2641 cgcttcgcat ggagaaggac gcggtcatca tcctcgatcc ggtgaaccgc ccggtcatcg
     2701 acaaggcgct ccaatccggc gtgaagaact tcatcggcgg gaactgcacc gtgagcctca
     2761 tgctgatggc gatcgatggc ctcctgaagg ccgatctcgt cgagtgggtg agcgccatga
     2821 cgtaccaggc ggcctcgggg gccggcgccc aaaacatgcg agagctcctg caacagatgg
     2881 gcgaggtgca tttcgcggcg aagagccttc ttgccgatcc ggcctcggcg attctcgaca
     2941 tcgatcggga agtcgccggg attctccgcg acgagagctt ccccacggag cattttggcg
     3001 tgccgctcgc ggggagcctc ctgccgtgga tagacgccga cctcggcaat gggcagagcc
     3061 gcgaggagtg gaagggacac gcggagacca acaagatcct gggacgcgaa gctcgtcctg
     3121 tcgcggtcga cgggctgtgc gtgcgcgtcg gcgcaatgcg atgccacagc caggccctca
     3181 ccatcaagat gaagcgcgaa ctgccggtgg aagagatcga gtcgatcctt gcaaagggca
     3241 acgcgtgggt gaaggtggtg ccgaatcgcc gcgaagagac tttgcgccgg ctcacgccgg
     3301 tcgccgtgac gggtacgctc gatgtgccga tcggtcgagt tcgcaaattg cggatgggag
     3361 gcgagtatct ttcagctttt acagtgggcg atcaattgct ttggggggcc gcagagccgc
     3421 tgcgccggat gctgggtatc ctgctgaagg cccgggttgc tgtaggcgct gccgcgtaaa
     3481 cccccgttcc attaggaaaa agtggttgaa aaatgtccca cttaagagat aatgtgaaaa
     3541 tccgggtgag atagaaacga taagcccttg atgccacgac cttttcaccg at
//
LOCUS       DASVII010000760         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_215271,
            whole genome shotgun sequence.
ACCESSION   DASVII010000760 DASVII010000000
VERSION     DASVII010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215271"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VF309_09900"
     CDS             complement(<1..381)
                     /locus_tag="VF309_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7466944.1"
                     /translation="MSRQSLPDFSRARVLVAGDVMLDRYWFGDVSRISPEAPVPVVHV
                     QRTEERPGGAANVARNITALGGGATLLAVVGADEAGDSLERLLGVEKVAASLHRDPSL
                     STTVKLRVIGQQQQLLRIDFERAPS"
     gene            complement(398..1732)
                     /locus_tag="VF309_09905"
     CDS             complement(398..1732)
                     /locus_tag="VF309_09905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX7466945.1"
                     /translation="MKASIIGTGYVGLVTGACLADVGNHVLCVDVDERKIGMLRAGEI
                     PIFEPGLKEIVRSNVASGRLAFTTDAKESATYAKLQMIAVGTPPGEDGSADLQYVIAA
                     ARSIATHMDGPRVIVDKSTVPVGTADKVRDAVAAALKERGAAHPFAMVSNPEFLKEGA
                     AVEDFMRPDRIVIGAEDPDAIATMRELYGPFQRNHERLQVMDIRSAELTKYAANAMLA
                     TRISFMNELALLAERLGADIEQVRIGMGSDPRIGYHFLYPGAGYGGSCFPKDVTALIR
                     TGEENGIELKVVRAVEEANQRQKSVIAEKIARRFGENLKGKRFALWGLAFKPNTDDMR
                     EAPSLVIIAALLERGAQVVAYDPVAMPEARHIYERNGAVAFAEDAMGAVSGADALVII
                     TEWKAFRSPDFEALKRALKTPVIFDGRNLYEPAVVRGHGLEYYPIGRGRYET"
     gene            complement(1729..>1911)
                     /locus_tag="VF309_09910"
     CDS             complement(1729..>1911)
                     /locus_tag="VF309_09910"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX7466946.1"
                     /translation="TPGIRLNTDPKDDQARTVTPVDAVRLGAHYLVIGRSITKAADPA
                     ATLAAIRASIEEGAAA"
BASE COUNT          272 a          661 c          656 g          322 t
ORIGIN      
        1 gctgggcgcg cgctcgaaat cgatcctgag gagctgctgc tgctggccga tcacgcgaag
       61 cttcaccgtg gtcgagagcg aagggtcgcg gtgcagggac gcggcgacct tctcgacgcc
      121 gagaaggcgc tcgagggaat cgccggcctc gtccgctccc accacggcga gcagcgtcgc
      181 gccgcccccg agcgcggtga tgttgcgcgc gacgttggcc gcgccaccag gtcgctcctc
      241 ggtgcgctgc acgtgcacga cgggcaccgg agcctcgggc gagatgcggc tcacgtcgcc
      301 gaaccagtag cggtcgagca tgacgtcgcc ggccacgagg actcgcgcgc gcgaaaaatc
      361 cggaagcgat tggcgactca tgcgcgcgcc ccgtctctca cgtctcgtac cgcccgcgcc
      421 cgatggggta gtactccagg ccgtggccgc gaacgaccgc cggctcgtag aggttgcgcc
      481 cgtcgaagat caccggcgtc ttcagggcgc gcttcagcgc ctcgaaatcg gggctgcgga
      541 atgccttcca ttcggtgatg atcacgagag cgtcggcgcc actcacggcc cccatcgcgt
      601 cctcggcgaa cgccaccgcg ccgttccttt cgtagatgtg ccgcgcctcg ggcatcgcca
      661 ccggatcgta ggcgacgacc tgcgctccgc gctccaggag cgccgcgatg atgacgaggc
      721 tcggcgcctc gcgcatgtcg tcggtgttgg gcttgaaggc gaggccccag agcgcgaagc
      781 gcttcccctt caggttctcg ccgaagcggc gcgcgatctt ctcggcgatc acgctcttct
      841 ggcgctggtt ggcctcctcg acggcccgca cgaccttcag ctcgatgccg ttctcctcgc
      901 cggtgcggat gagcgcggtc acgtccttgg gaaagcacga gccgccgtag cccgcgcccg
      961 ggtagaggaa gtgatagccg atgcgcggat cggagcccat gccgatgcgc acctgctcga
     1021 tgtccgcgcc gagccgctcg gcgaggagcg cgagctcgtt catgaaggaa atgcgcgtgg
     1081 cgagcatcgc gttggccgcg tacttggtga gctccgccga gcggatgtcc atcacctgca
     1141 ggcgctcgtg gttgcgctgg aacggcccgt agagctcgcg catcgtggcg atcgcgtcgg
     1201 gatcttccgc gccgatcacg atgcgatcgg gacgcatgaa atcctccacc gcggcgccct
     1261 ccttgaggaa ctcgggattg gagaccatgg cgaaagggtg cgcggcgccg cgctccttga
     1321 gggccgccgc caccgcgtcg cgcaccttgt ccgcggtgcc gacgggaacc gtggacttgt
     1381 cgacgatgac gcgcggaccg tccatgtgcg tcgcgatgct gcgcgccgcc gcgatcacgt
     1441 attgcaggtc cgccgagccg tcttccccgg gaggcgtgcc caccgcgatc atctgcagct
     1501 tcgcgtaggt cgcgctctcc ttcgcgtcgg tggtgaacgc gagccggccg ctcgccacgt
     1561 tgctgcgcac gatctccttc agtcccggct cgaagatcgg gatctctccg gcgcgcagca
     1621 tgccgatctt gcgttcgtcg acgtcgacgc acagcacgtg gttgccgacg tccgcgaggc
     1681 aggcccccgt gacgaggccg acatagcctg ttccgatgat gctcgccttc atgccgcggc
     1741 cccttcctcg atcgacgcgc ggattgccgc gagcgtggcg gccggatcgg cggccttggt
     1801 gatggagcgc ccgatcacga gatagtgggc gccgaggcgc acggcgtcca cgggcgtcac
     1861 ggtgcgcgcc tggtcgtcct tgggatctgt gttcaatcga attcccggtg t
//
LOCUS       DASVII010000761         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_431419,
            whole genome shotgun sequence.
ACCESSION   DASVII010000761 DASVII010000000
VERSION     DASVII010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431419"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF309_09915"
     CDS             complement(<1..547)
                     /locus_tag="VF309_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7466947.1"
                     /translation="MTQTISLDAVPRPAGGYFFEDYAVGRRFHHATPRTLTEADASLY
                     IGLTGARQPVHCAEPVARALGHPSCPVDELLVFHIAFGKTVPDVSYNAIANLGYADVR
                     FLAPVYGADTLSCESAVLGVKENSDGKSGVVYVRSRAFNQDRAEVLAWTRWVMVARRN
                     ARGDDRALAPVIPALADHVEPS"
     gene            complement(544..1422)
                     /locus_tag="VF309_09920"
     CDS             complement(544..1422)
                     /locus_tag="VF309_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7466948.1"
                     /translation="MNPYYRPRRSMLYVPGCNVRYLNKARTLRVDSVILDLGDPILVE
                     KKVQSRKLVADAVLAGGYGRREVVVRVNDLDSLWGRDDVKTVATIGADAVLFPNIESK
                     KDVLEAQALLDEAGGSHMPIMLMIESPLGVLRAEEIASASDRIACIVLATSDLLNQLH
                     GRRTPDRIALVHSLSHVLLAGRAYDRAVVDGISTDLKDMQGFELACRLGRDLGFDGKS
                     LVHPFQLPYCNDAFTPKPHDLAAALEVIDALEQAHRDGRGTVVVNGRLIEGHHVKAAK
                     RLLALSDMIQKLEAGQ"
     gene            1528..>1720
                     /locus_tag="VF309_09925"
     CDS             1528..>1720
                     /locus_tag="VF309_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7466949.1"
                     /translation="MKKSATTPSFRPLYEQIKILLTQSLVAGEWKPGEAIPSEMDLAE
                     RFRVSQGTVRKAIDELASEH"
BASE COUNT          313 a          551 c          584 g          272 t
ORIGIN      
        1 ccgacggctc cacgtggtcg gcgagcgcgg gaatcaccgg cgccagcgcg cgatcgtcgc
       61 cgcgcgcgtt gcggcgcgcg accatgaccc agcgggtcca cgcgagcact tccgcccggt
      121 cctggttgaa ggcacgcgag cgcacgtaga ccacgccgct cttgccgtcg gaattctcct
      181 tgaccccgag cacggccgac tcgcacgaaa gcgtgtcggc cccataaacc ggcgcgagga
      241 atctcacatc cgcgtagccg aggttcgcga tcgcgttgta cgacacgtcc gggacggtct
      301 tgccgaacgc aatgtggaac acgagcagtt cgtccaccgg acacgagggg tggcccagag
      361 cgcgggccac cggctccgcg cagtgcaccg gctggcgcgc gccggtaagg ccgatgtaga
      421 gggacgcgtc cgcctcggtg agcgtgcgcg gcgtcgcgtg atggaagcgc cgtccgaccg
      481 cgtagtcctc gaagaaatag ccgcccgcgg ggcgtggcac cgcgtcgagg gagatcgtct
      541 gggtcactgc cccgcctcga gcttctggat catgtcggag agcgcgagga gccgcttcgc
      601 cgccttcaca tggtggccct cgatgagccg gccgttcacc accaccgttc cacggccgtc
      661 gcgatgcgcc tgctcgagcg cgtcgatcac ctcgagcgcc gcggcgaggt cgtggggctt
      721 gggcgtgaac gcgtcgttgc aatagggaag ctggaacgga tgcacgaggc tcttgccgtc
      781 gaagccgaga tcgcggccga ggcggcacgc gagctcgaag ccctgcatgt ccttgaggtc
      841 cgtcgagatg ccgtcgacca ccgcgcggtc atacgcgcgc cccgcgagca gcacgtgcga
      901 caggctgtgg acgagggcga tgcgatcggg cgtgcgccgg ccgtgcagct ggttgagcag
      961 atcggaggtg gcgagcacga tgcacgcgat ccgatcggag gccgatgcga tttcctcggc
     1021 gcgcagcacg ccgagcgggc tttcgatcat gagcatgatc ggcatgtggc tgccgcccgc
     1081 ttcgtcgagc agcgcctgcg cttcgagcac gtccttcttc gactcgatat tggggaagag
     1141 cacggcgtcg gcgccgatgg tcgcgacggt cttcacgtcg tcgcgtcccc acagggaatc
     1201 gagatcgttc acgcgcacca cgacttcgcg gcgtccgtat ccgcccgcga gcaccgcgtc
     1261 ggcgaccagc ttccgcgact ggaccttctt ttccacgagg atggggtcgc ccaggtcgag
     1321 gatcaccgaa tcgacccgca aggtgcgcgc cttgttcagg tagcgcacgt tgcaccccgg
     1381 aacgtagagc atggagcgac gcgggcggta gtacgggttc atgcgctaag tccctgaaag
     1441 gcagatgtaa aaattctcgc ggatgaagcg aagaagatgt atcttatata agacatccac
     1501 aaaaagctat gctaggatgc ccaaagcatg aaaaaatccg cgacgacgcc ctcgttccgg
     1561 ccactatacg agcagatcaa gatcctgctc acccagtcgc tggtggcggg agagtggaaa
     1621 ccgggcgagg cgattccctc ggagatggac cttgcagagc gcttccgggt gagccagggc
     1681 acggtgcgca aggcgattga cgagctcgca tcggaacaca
//
LOCUS       DASVII010000762         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_215549,
            whole genome shotgun sequence.
ACCESSION   DASVII010000762 DASVII010000000
VERSION     DASVII010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215549"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VF309_09930"
     CDS             complement(<1..494)
                     /locus_tag="VF309_09930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7466950.1"
                     /translation="MSGRGPVAVDPLDRHPRLAPRLCEEFTREWPAWCGSVTRARLEA
                     CFASAPDGGLPIVFVAHDHGVPLGTVSLRPWFAEEPMTESPWVRGLLVFPEFRGGAVL
                     RALESAVEAYARDSGFAHLHAGVTSMEPMLLRRGWKAFRRVSHEGKDMAWMRNLVSET
                     NFPGA"
     gene            complement(491..>1684)
                     /locus_tag="VF309_09935"
     CDS             complement(491..>1684)
                     /locus_tag="VF309_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7466951.1"
                     /translation="RKVAIKVSFPEALKHSEDGAFYKSMFLNEAALAGKLNHPHITQI
                     YDAVVEDKYSYIVMEYVEGGTLEKFCKPEALLETRDVAEISFKCVRALAFAQTLGLTH
                     RDIKPGNILHSDGTEIKIADFGAAINKVSDRTVITNVGSPAYMAPELVTGQAQASHLT
                     DIYALGVVMYYLLAGKLPFSGANTLSVIYQIVNTDPEPPSTHRKGISAELDAVVMKAM
                     AKDPAKRFATWDEFGAALAEIWKKEHKAGEKRDQSDTERFSLMRTLPFFREFPENELW
                     EVLKLTKWAKFKPDTALIKEGDQGDSFYVLAGGYVRVTRGKKTLSVLTAGDCFGEMSY
                     LAKTGDPHRSATVTTTSDAIIMKIRAEDLRGASLSCRRLFDERFLNTLVERLEKANEQ
                     ISVMS"
BASE COUNT          269 a          557 c          565 g          293 t
ORIGIN      
        1 gcaccgggaa aattggtctc tgacaccaga tttctcatcc atgccatgtc cttgccttcg
       61 tgcgagacgc ggcgaaaggc cttccagccg cggcgcaaga gcatgggctc catcgaagtg
      121 actccggcgt ggaggtgcgc gaagccgctg tcgcgcgcat aggcttccac cgcgctctcg
      181 agcgcgcgca acacggcgcc gccgcgaaac tcgggaaaca cgagaagccc gcgcacccaa
      241 ggcgactcgg tcatgggctc ttccgcgaac cacggccgca gcgaaacggt cccgagggga
      301 acgccgtggt cgtgcgcgac gaagacgatg ggcagcccgc cgtcgggcgc gctcgcgaag
      361 cacgcctcga gccgcgcgcg cgtcaccgag ccgcaccagg ccggccactc gcgcgtgaat
      421 tcctcgcaga ggcgcggcgc gaggcgcgga tgccggtcga gcggatcgac cgcgaccggc
      481 ccgcgcccgc tcacgacatc acggagatct gctcgttggc cttctcgagc cgctcgacga
      541 gcgtattgag gaagcgctcg tcgaacagcc ggcggcacga gagcgacgcg ccgcgcaggt
      601 cctcggcgcg gatcttcatg atgatcgcgt cggaggtcgt ggtgacggtc gcggaacgat
      661 gggggtcgcc cgtcttggcg aggtacgaca tctcgccgaa gcagtcgccc gccgtcagca
      721 cggagagcgt cttcttcccg cgcgtgacgc gcacgtaccc gccggccagc acgtagaagg
      781 aatcgccctg gtcgccttcc ttgatgagcg cggtgtcggg cttgaacttc gcccacttgg
      841 tgagcttcag gacttcccag agctcgtttt cgggaaactc gcggaagaac ggcagcgtgc
      901 gcatgaggct gaaacgctcc gtgtccgact ggtcgcgctt ctccccggcc ttgtgctcct
      961 tcttccagat ctcggccagc gccgcgccga actcgtccca cgtcgcgaag cgcttcgcgg
     1021 ggtccttcgc catcgccttc atcaccacgg cgtcgagctc ggcggagatg cccttgcgat
     1081 gcgtggacgg cggctccggg tcggtgttca cgatctggta gatcaccgag agcgtgttcg
     1141 cgccggagaa cgggagcttc ccggcgagga ggtagtacat cacgacgccc agcgcgtaga
     1201 tgtcggtaag gtgcgatgcc tgcgcctgcc cggtgaccag ctccggcgcc atgtacgcgg
     1261 gtgagccgac gttggtgatc accgtgcggt cgctcacctt gttgatcgcg gcgccgaagt
     1321 cggcgatctt gatctcggtg ccgtcgctgt gcaggatgtt gccgggcttg atgtcgcggt
     1381 gcgtgaggcc gagcgtctgc gcgaaggcga gcgcccgcac gcacttgaag ctgatctccg
     1441 ccacgtcgcg cgtctcgagg agcgcctcgg gcttgcagaa cttctcgagg gtgccgccct
     1501 cgacgtactc catcacgatg tagctgtact tgtcctcgac caccgcatcg tagatctgcg
     1561 tgatgtgcgg gtggttcaac ttgccggcga gcgcggcctc gttcagaaac atgctcttgt
     1621 agaacgcgcc gtcctcggag tgcttcaacg cctcggggaa ggagaccttg atcgcgacct
     1681 tgcg
//
LOCUS       DASVII010000763         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Usitatibacter sp. isolate SMAG_U2989 k141_107233,
            whole genome shotgun sequence.
ACCESSION   DASVII010000763 DASVII010000000
VERSION     DASVII010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959621
            Sequence Read Archive: SRR10489816
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Usitatibacter sp. (soil metagenome)
  ORGANISM  Usitatibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Usitatibacteraceae; Usitatibacter.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 22:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,989
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 6
            tRNAs                             :: 4
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 12 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            Pseudo Genes (multiple problems)  :: 2 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Usitatibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107233"
                     /isolate="SMAG_U2989"
                     /isolation_source="soil"
                     /db_xref="taxon:2803847"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /locus_tag="VF309_09940"
     CDS             complement(<1..2315)
                     /locus_tag="VF309_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7466952.1"
                     /translation="MWITRTSINQPVFATMVMVALLVLGVFSYNRLRVERMPEINFPV
                     VFVSVQYPGASPEAVENDITRPIEEVVNTVNGVRLIRSNSFEGRSETGIEFRLDANIE
                     RGVQDVRDKIALIRPGFPREVRDPLVLRGDFDNAQPVVSLAITSPTRSLRELTTMTDQ
                     VIVKRFQNVPGVGQVQESGGVARQILVNLKPAQMLSQGVGVDEVVRAIQSANMDTPAG
                     RVVQGPSEQLVRVEGKIKEPAAFAKIIVARRAKGPVYLGEVADIVDGEREPESLSRVN
                     GNPGLSLNVLKVQDANIVEVGAGVRAAVEDLRKTLPADVKIEIAYADVDFIEKALNGV
                     KTTIVEGAILTVLIVFLFLHSWRSTIITGLTLPISVIATFIAVYVFGFTLNFLTLMAL
                     SLCIGLLIDDAIVVRENIVRHLGMGKDHFRAAREGTDEIGLAVMATTFAIVAVFVPVA
                     FMKGIIGKFFFQFGITVAVAVLVSLFVSFTLDPMLSSIWRDPPKGRFARLPLLARMMD
                     RIEAAIEWAHERYGALLEWSLENRLKVMAFAVVVFIGSFFIVPLVGTEFVPETDEGRS
                     SFRLTTPVGSSLEYTDAKARQVEELLRRYPEVEMMIANVGTGDGRNVARVDVRLVDRH
                     VTPRISQKAFEAAVRDKIRAIPGIELSVGYQKPIWINLLGPSPEGLADVTQEIVDKLG
                     KIKGITDLETSNKAQNPAITIKVNNELASDLGLTVAQIGAAVRPFVAGDVISHWLAPD
                     GQNYDVNVQLPRTSRRIAADLGELHVMSSRV"
     gene            complement(2324..>3061)
                     /locus_tag="VF309_09945"
     CDS             complement(2324..>3061)
                     /locus_tag="VF309_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7466953.1"
                     /translation="NRENGEALLRQKFISQNAFDTTHSTYEASAASVRSAEAQLRIAR
                     KAMDDASVRAPFNGIVARKLVNAGEKVGIDSPLFGLVDLANMEIEAPAPAAEIPAVKP
                     GQAASFRVDGFGERVFTGKVERINPSADAGSRSITLYISVANQDGALKGGMFAKGDIV
                     LESTAISTVIPASAVRVEAGQEYVFTIEGGKIGKRAVKLGATEPQRGLVEVRSGLEEG
                     ISVVSARVSGLKPGAPAVIKTAAAKAA"
BASE COUNT          479 a         1017 c         1036 g          529 t
ORIGIN      
        1 ttcacgcggc tgctcatcac gtggagctcg cccaggtcgg cggcgatgcg ccgcgaggtc
       61 ctgggcagct ggacgttcac gtcgtaattc tggccatcgg gcgcgagcca atggctgatc
      121 acgtcgcccg cgacgaaggg gcgcaccgcc gcgccgatct gcgccaccgt gaggccgagg
      181 tcgctcgcca gctcgttgtt caccttgatg gtgatcgcag ggttctgcgc cttgttgctc
      241 gtctcgaggt cggtgatgcc cttgatcttc ccgagcttgt cgacgatctc ctgggtgacg
      301 tcggcgaggc cctcgggcga cgggccgagg aggttgatcc agatcggctt ctggtagccc
      361 accgagagct cgatgccggg aatggcgcgg atcttgtcgc gcaccgccgc ctcgaacgcc
      421 ttctgcgaga tgcgcggcgt cacgtggcga tccaccagcc gcacgtcgac gcgcgcgacg
      481 ttgcggccgt cgccggtgcc gacgttggcg atcatcatct cgacttcggg atagcggcgc
      541 aggagctcct cgacctggcg cgccttggcg tcggtgtatt cgaggctgga ccccacgggc
      601 gtggtgaggc ggaatgagct gcggccctcg tcggtctcgg ggacgaactc ggtgccgacg
      661 agcggaacga tgaagaagct cccgatgaac acgaccaccg cgaacgccat caccttgagg
      721 cgattctcga gcgaccattc gaggagcgcg ccgtagcgct cgtgggccca ttcgatggcc
      781 gcctcgatgc ggtccatcat ccgcgcgagg agcggcaggc gcgcgaaccg gcccttcggg
      841 ggatcccgcc agatcgacga cagcatggga tcgagggtga agctcacgaa gagcgacacc
      901 agcacggcga ccgcgacggt gatgccgaac tggaagaaga actttccgat gatgcccttc
      961 atgaacgcca ccgggacgaa cacggcgacg atcgcgaacg tggtcgccat cacggcgagc
     1021 ccgatctcgt cggtgccctc gcgtgcggcg cggaagtggt ccttccccat gccgaggtgc
     1081 cgcacgatgt tctcgcgcac cacgatcgcg tcgtcgatga gcagcccgat gcacagcgac
     1141 agcgccatca gcgtgaggaa gttcagcgtg aagccgaaca cgtagaccgc gatgaaggtc
     1201 gcgatcaccg agatcggcag cgtgagcccg gtgatgatgg tgctgcgcca cgaatgcagg
     1261 aagaggaaga cgatgaggac cgtgaggatc gcgccctcga cgatcgtcgt cttcacgccg
     1321 ttcagcgcct tctcgatgaa gtcgacatcg gcgtaggcga tctcgatctt cacgtcggcg
     1381 ggcagtgtct tcctcaggtc ctcgaccgcg gccctcacgc cggcgccgac ttccacgatg
     1441 ttcgcgtcct gcaccttgag cacgttgagg ctcagtccgg ggttgccgtt gactcgcgac
     1501 agcgactcgg gctcgcgttc cccgtcgacg atgtctgcga cctcgccgag atagacgggg
     1561 cccttcgcgc ggcgcgccac gatgatcttc gcgaacgccg ccggctcctt gatctttccc
     1621 tcgacgcgca ccagctgctc gctcggcccc tgcaccacgc ggcccgcggg cgtgtccatg
     1681 ttggcggact ggatcgcgcg gacgacctcg tcgacgccga caccctgcga gagcatctgc
     1741 gcgggcttca ggttcacgag gatctggcgc gcgacgccgc ccgattcctg cacctggccc
     1801 acgccgggaa cgttctggaa acgcttgacg atgacctggt cggtcatcgt ggtgagctcg
     1861 cgcaggctgc gggtgggcga ggtgatcgcg agcgagacca ccggctgcgc gttgtcgaaa
     1921 tcgccgcgca gcacgagcgg atccctcacc tcgcgcggaa agcccgggcg gatgagcgcg
     1981 atcttgtcgc gcacgtcctg cacgccgcgc tcgatgttcg cgtcgaggcg gaactcgatt
     2041 cccgtctcgc tgcggccctc gaaggagttc gagcggatga ggcgaacgcc gttcacggtg
     2101 ttcacgactt cctcgatggg cctcgtgatg tcgttctcga ccgcttcggg cgaggcgcct
     2161 ggatactgca cgctcacgaa gaccacggga aagttgatct ccggcatgcg ctcgacgcgc
     2221 agccggttgt acgagaagac cccgaggacg aggagcgcca ccatcaccat ggtggcgaac
     2281 acgggctggt tgatgctggt gcgggtgatc cacatggcgg ggcctatgcc gctttcgccg
     2341 cggccgtctt gatcaccgcg ggggcgccgg gcttcaggcc ggagacgcgc gcgctcacga
     2401 cgctgattcc ttcctcgagg ccgctgcgaa cctcgacgag gccgcgctgc ggctcggtgg
     2461 cgccgagctt caccgcgcgc ttgccgatct tgccgccctc gatcgtgaag acgtattcct
     2521 gccccgcctc gacgcgcacc gcgctcgccg ggataaccgt ggagatcgcg gtggattcga
     2581 gcacgatgtc gcctttcgcg aacatcccgc ctttcaacgc gccgtcctgg ttcgcgacgg
     2641 atatgtagag cgtgatggag cgcgagcccg cgtccgcgga gggattgatg cgctccacct
     2701 tcccggtgaa gacgcgctcg ccgaagccgt cgacgcggaa gctcgcggcc tggcccggct
     2761 tcacggcggg tatctcggcg gcgggcgcgg gagcctcgat ctccatgttg gcgaggtcga
     2821 cgagcccgaa gagggggctg tcgatgccga ccttttcgcc cgcattcacc agcttgcgcg
     2881 cgacgatgcc gttgaacggc gcgcgcaccg acgcgtcgtc catagccttg cgcgcgatgc
     2941 gaagctgcgc ctccgccgag cgcaccgatg ccgcgctcgc ctcataggtg ctgtgcgtcg
     3001 tgtcgaaggc gttctgggag atgaacttct ggcgcagcag cgcctcgccg ttctcgcggt
     3061 t
//
